LOCUS       DAOSFN010000001        69789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_308_length_69789_cov_9.699444, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000001 DAOSFN010000000
VERSION     DAOSFN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 69789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 69789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69789
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_308_length_69789_cov_9.699444"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..75)
                     /locus_tag="PLO35_00005"
     tRNA            complement(1..75)
                     /locus_tag="PLO35_00005"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Gln,seq:ttg)
     assembly_gap    139..151
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(426..1763)
                     /locus_tag="PLO35_00010"
     CDS             complement(426..1763)
                     /locus_tag="PLO35_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOH78072.1"
                     /translation="MIRFEYRNLLHSKHIPTAIAPERIADMSSAILDAHDEIGKDRQA
                     DILGFYALPEQNVSHITDFVAGLDPRFDTMVVLGIGGSALGNKALYSALKTERELPRR
                     VLVYDNVDPVFLKEILDQIDLDRTLFNVITKSGTTAETMAGYMILADIVQKRFPQDYK
                     KRIVVTTDREKGFLRQVIRKEGYPSFVVPDNVGGRFSVLTDVGLLSSAFAGMDIAKLL
                     EGAKAMRSRCEDTDIYSNPAYLNGLLHYLYMREGKNISVMMPYSNALYDMADWYRQLW
                     AESLGKRRDTRGREVFVGQTPVKALGTTDQHSQVQLYAEGPMDKVFTFLTVDEFAHDY
                     VIPNLHPEREEVSYLGGRKLSELLNAEALATAVALTSAGRPNATIGFPRLDEYHLGEF
                     IMMYEIQTVFTGKLLHIDPLDQPGVEAGKIATYALMGKKGFEAELNRIKAMTT"
     gene            complement(1773..3494)
                     /gene="ptsP"
                     /locus_tag="PLO35_00015"
     CDS             complement(1773..3494)
                     /gene="ptsP"
                     /locus_tag="PLO35_00015"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOH78073.1"
                     /translation="MRILTGNSINPGIFIGRARFLSSRSPVVLHSHINPENAEQEFQG
                     LLQAIETAVLQLDELLDALPKTGSEREIFETHQIILRDPDLLAKLEGLVLIDYNSAAQ
                     AVKTAFDEVASMFTAMQNDFFAQRAADYRDLQERLLAILLGHSADPLAVFSSDEVLFC
                     PEPSPSLVTAMASKGIGAWVSQKGSYTSHAAILTRGLDIVALSAIADLSEQLDNGQTV
                     IVDALGAQLIIDPDEDTLLRFSELQAKFRTARQKDLADALLPAVTQTGKMIRVKGNIE
                     LPAEASQLRALGAEGIGLFRTEFLYLDRSALPSEDEQYQIYTQVLEAMAPYPVTIRSF
                     DLGGDKLSHLIPSEREDNPYLGCRGLRFSLFRPDVFKTQLKAALRAAVHGELSLMFPM
                     VNDASDLISAKEIVKKCAAELRERGIPCRADIPMGVMIEVPSAALCADELARYADFFS
                     IGTNDLAQYTLAVDRNSDALSGRYTQHHPAVLKLIALSISAAHKHGIAVSVCGEMGSI
                     PHYIPLLVGMGIDELSVHPKRVAFCKNIIRRCDSELTSILGTSERQDLAAVEQLINHD
                     LKRYYQD"
     gene            complement(3744..4010)
                     /locus_tag="PLO35_00020"
     CDS             complement(3744..4010)
                     /locus_tag="PLO35_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOH78074.1"
                     /translation="MIQKKIKVSNKLGLHARPSALMVRAATKFRSEFFIEKDGMRVNG
                     KSIMGVMMLAAESGSTLNLIADGVDESYLIEEVSALITSGFGED"
     gene            complement(3982..4968)
                     /gene="hprK"
                     /locus_tag="PLO35_00025"
     CDS             complement(3982..4968)
                     /gene="hprK"
                     /locus_tag="PLO35_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOH78075.1"
                     /translation="MREITVKELFDNKKKDLALSLVTEPETLNKKISSPHVNRPGLAL
                     TGWLEVFASDRIQVIGEAEVRYLQSLKEELLIQRARDLFKTDIPCVIVTKGLTLPPVV
                     EYLANDLNIALLSSRFSTIQLIHQLTRYLRDVFALEKTIHATLVDVFGLGIMLTGKSG
                     IGKSECALDLIHRGHSLVGDDLITIRYIDDQLVGKSGREFGHFMEIRGVGFVNVERMF
                     GIERVRRQKTIDMQIELMPWQENMDYERIGLTSNYADHLGVKLPIIYLPVSPGKNVSV
                     IVEVAAMNMILKSVGYDAAEDFNRRLHEEIRKQTLLKQTQAQGNDTKEDQGQ"
     gene            complement(5043..5579)
                     /gene="raiA"
                     /locus_tag="PLO35_00030"
     CDS             complement(5043..5579)
                     /gene="raiA"
                     /locus_tag="PLO35_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOH78076.1"
                     /translation="MQITITARHFELTKAIRDYVETSCLKVKKYFDHIIHVHVTLALE
                     NNRNICELTLHASRFNLQSQAEEMDMYLSIDNALDKMEAQIKKLKDRVTDHQKKALKE
                     QFNDFSRATEIQVNTESRARKTLKTKRVVPEIMDVQEALEKIDELKEEFLIFKNVETD
                     RLNVLVKRDAEHYKLFEP"
     gene            complement(5598..6998)
                     /gene="lpdA"
                     /locus_tag="PLO35_00035"
     CDS             complement(5598..6998)
                     /gene="lpdA"
                     /locus_tag="PLO35_00035"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOH78077.1"
                     /translation="MQQYQVAIIGGGPGGYETAIRLSQYEISCVVIEAERVGGVCLNW
                     GCIPTKALVKSAELFRELKEAESYGLPVSDGLLDYSRVFERKNQIVEQLVSGIEFLFR
                     KRRIPIVKDKATAISKVGELWKVDLAEGESISAQWIILATGSEPKSLPGIAIDEVNVL
                     SSTGILKLDKLPSSLAVIGGGVIGCEFASIMNSFGVKVHIIEFLPRIVALEDEELSKR
                     LALALKKAGIKITTGAGLTSALWSGDGMLLTLSDGSELMVDKVLMSVGRSPVMNIAWP
                     QTAPQMERGAVVIDAWMRTSLPGIYAIGDVTAKLPLAHTASKQGLIVASHIHSQVSQQ
                     DFHLLALDYVNVPRCTFTDPELASVGYTEAEAKERFSDILVGKFPFSASGKALALGNS
                     FGFVKTIARADTKALVGMHILGPNAAELIAQGAILISQGATAEAVESIVFAHPTLSEA
                     VKESLEDIRNLSIHKI"
     gene            complement(7003..8460)
                     /gene="rpoN"
                     /locus_tag="PLO35_00040"
     CDS             complement(7003..8460)
                     /gene="rpoN"
                     /locus_tag="PLO35_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOH78078.1"
                     /translation="MSGLDQHLSLRQKQELALKPKMLQSLKMLSLPILELESYIKLEL
                     VNNPLLELREEKDEEDIDESGFEASQPESSVSIEEKEEGEESFKTISEARELTEILDQ
                     WNEYHLSYESRHGDSEADHTESLIRFEEDGREKFLEQLFPLDLTDAELDFCSELVDSC
                     DIYGFLPLRFDLEKTARRYRIPASRANELHQIILHLSPRGISSRDIGECLLSQLTDDQ
                     LQNRIIVGLCTDQFENLIHRRYQKLASHFSVSEETIFNAREQISHLDPKPGLRILAPN
                     TSFVFPDVTWKLIDGEYVVIINDQITPNIIISPRYRKMISRGAFDRETLQFVRDRINS
                     AKFLIKSIFMRMRTLERVSLSIIKHQADFFYSGLGVLQPLTYAVIAQDIGVSESTISR
                     VVKHKYAETPYGIFALKDFFSSTAGMDDNYETISRQHVKSHIIRMVELEDKRHPLSDQ
                     ELVENLKALGMNISRRIIAKYRFELGILNSRLRRS"
     gene            complement(8463..9206)
                     /gene="lptB"
                     /locus_tag="PLO35_00045"
     CDS             complement(8463..9206)
                     /gene="lptB"
                     /locus_tag="PLO35_00045"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOH78079.1"
                     /translation="METHKIRAQHLVKIYGRRTVVNDLCMDMSQGEVVGILGPNGAGK
                     TTTFYMIIGLAKPNKGKVMLDDRDITHLPMYRRARLGLGYLAQAPSIFAKLSVQDNIL
                     AILQTLKLPKAEQKKRLAEALEELGLTKLAKQKAYTLSGGERRKLEITRALVTNPAFI
                     FMDEPFAGVDPIAVADIQDIIGKLRDKNIGIMITDHNVIETLKIVNRAYIIYEGKIIV
                     SGTSMELINDEKAKQVYLGDRFLSNPFDK"
     gene            complement(9238..10299)
                     /locus_tag="PLO35_00050"
     CDS             complement(9238..10299)
                     /locus_tag="PLO35_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOH78080.1"
                     /translation="MLLVVLLPLGAQTTSKTEAFKLIHSDKLFLNRLNNEQVLELSGK
                     VHFWYGKTEFKSDRAMIFDTQKIARLSGRVSVVNDSLGLKADSVAYYRSTEDLNLGGK
                     VQITESRKTGSLRWFKSEYAIYNRKADKLTVWKDVSAWDKDENAWAECGYAYWDRKAG
                     YAYMIENPRLRSGVADTLYIKADKIEFFDADRKIVATFNAEVKSQDYLVNSDFLIYMI
                     NEEKAVFTGRPSFLSDYAKASANEFYLYLKERKLQRAELQDSCRVSFSEERGGIQDNW
                     VTAGFISIGFREEAIKEFTAEDEVVYYYAQAARDKRDPFVNTARGYFLEAKFNDDNKL
                     ELMRMARGVKGSYKFQNNP"
     gene            complement(10406..10924)
                     /gene="lptC"
                     /locus_tag="PLO35_00055"
     CDS             complement(10406..10924)
                     /gene="lptC"
                     /locus_tag="PLO35_00055"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOH78081.1"
                     /translation="MLLALLLCSCKQQDLTVAAAPLDRNLPDESSNKVNIIEYNGDRV
                     DYEIFADRVDRFYDRRLMNAVGVEIRALDPKTGGTTHLKADSTIVDDARNMIFAYGNV
                     KLSSPGGMVSSNSMIWDRNLDEITAPGDVTVIRDGNTLRGQRLRTNPQIDFAELETVS
                     ADGNFDSTDFDW"
     gene            complement(10935..11555)
                     /locus_tag="PLO35_00060"
     CDS             complement(10935..11555)
                     /locus_tag="PLO35_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOH78082.1"
                     /translation="MAELNNKVILPHEYDQFAHQVTHFKKIGKPYKPRVNWDRINLII
                     FDCDGVLTDGKIIYGCNGEELKNFDAHDGMGFQLLRFSHVRTAVITGRSSAALERRCQ
                     DLKIDYLYQGVANKLEKTRELLKELKLDFCNLLYMGDDWNDIPVMNVAAVSVCPADAA
                     RDVAAMADYVMLASGGRGAVRECIELVLNNKTIYEQAVNDYLAKIS"
     gene            complement(11556..12365)
                     /gene="kdsA"
                     /locus_tag="PLO35_00065"
     CDS             complement(11556..12365)
                     /gene="kdsA"
                     /locus_tag="PLO35_00065"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOH78083.1"
                     /translation="MELYHRLKEAWPFFLIAGPCVVEGMDTMRQVAERLSFLSAEFKL
                     PLVFKSSYRKANRSSGSSPSGPGMEAGLRCLDELKREFGFPILTDVHECEEVKAVAQI
                     CDIIQIPAFLSRQTSLIEAVAKSGRIINIKKGQFMAPEDIIPAAIKARNAGSDQILLT
                     ERGTSFGYHDLVVDFRGFAIMAESGYPIVYDVTHSLQKPSLGQVSGGGPQYAAMMARA
                     AMATGKVRGLFIETHPQPHTALSDAASMLPLDDLKALLADAIAVRKSLKED"
     gene            complement(12375..13547)
                     /gene="metK"
                     /locus_tag="PLO35_00070"
     CDS             complement(12375..13547)
                     /gene="metK"
                     /locus_tag="PLO35_00070"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOH78084.1"
                     /translation="MTALHANLPCDKNEYVFTSESVSEGHPDKVCDQISDAVLDAFVG
                     KDPKARVACETLCTKNRVVLAGEISSPSQVKVEIEPLVRKVIADIGYTVDGKGFDDNC
                     YIENYLHKQEKALEQSNGAGDQGLMFGYACNQTRHLMPVPIAMAHKLLQKLSEARRNG
                     AIPQLMPDSKSQVSMRYCGRKPIALETVVISTHHHPLDDKGFQDLRQLIEELVIKPTI
                     AEMEADSFSTFSADKYAVKINPLGRWEDGGPAADTGLTGRKIIVDTYGGWAQHGGGAF
                     SGKDATKVDRSAAYMARHIAKSVVACGLADECLIQFSFVIGEQEPVSLMVDTYGSGVL
                     ADHEIEKIIRGNFDLTVNGIIDYLDLRRAIYLPTASYGHFGRDDASFSWEQIKKLS"
     gene            complement(13747..13822)
                     /locus_tag="PLO35_00075"
     tRNA            complement(13747..13822)
                     /locus_tag="PLO35_00075"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13787..13789),aa:Lys,seq:ctt)
     gene            complement(14058..15356)
                     /locus_tag="PLO35_00080"
     CDS             complement(14058..15356)
                     /locus_tag="PLO35_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOH78085.1"
                     /translation="MSLLESVKNKSVKIGVVGLGYVGLPMAVTVARKGYEVLGFEVSQ
                     YAIEHVNAGKNYIGDVEDAHLVEVVSSQKLSATDDYTRMKEVNIVLIAVPTPLDLYHQ
                     PDTTYIEACTKAMAPHLNKHTLIVLESTTYPGTTREIIVPELEKVGLKVGEDVYVAFS
                     PERVDPGNETYKTHNTPKVVGGMTPACNEIAKLFYESVLDAPVHLVSSPEVAEMEKIY
                     ENTFRNINIALANEMTVLCDRMGISIWEVVDAAKTKPYGFMAFYPGPGVGGHCIPIDP
                     FYLTWKAREFDFHTRLIELAGEINIAMPEFVVQRAIRILNKQGVAISRAKILLLGAAY
                     KKDIRDMRESPIEGVITQLEKVSADFEIHDPYVPEFHHEKNGRMYQTVSLGNLKKYDL
                     IILITDHSNLDYAKIAASGVAILDTRNAFKGITAENIELL"
     gene            15636..16082
                     /locus_tag="PLO35_00085"
     CDS             15636..16082
                     /locus_tag="PLO35_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOH78086.1"
                     /translation="MKLDELLLQADQDHNSGLNCAQSVLKQFCQSGGLPAEKYFLRLS
                     SGLGSGMKVGSVCGALSGGIMALGLHLGSEDPARKAEMDAPVRELVTRFRTTMAHKEC
                     NKIIGYDVSDPTQRAIAREKGIFASQCPLAIRTAVTIVYDILNRNQ"
     gene            complement(16455..17897)
                     /locus_tag="PLO35_00090"
     CDS             complement(16455..17897)
                     /locus_tag="PLO35_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78087.1"
                     /translation="MKYLIALVFCLYCAAVMANVSFQVSYTGFDALQMAAFEHAVSLW
                     EPLLDSDVPIKINARFQQIPGFVMVFVPNMVRNFTSAPQSNIWYCTAMANMIAGTELN
                     PGEADMDMFIVGTADHPWYYGLDDNCPAGSYDFVTEVFKAVAYGLGYMPSFYVQQGYG
                     SYGMLDPSVLGLTTSFPWEAMQGQPALYDTHIVNTQGQHLTDSAIFTNPSTTLNAQLT
                     GGNLRYEGQWAEAWGGGTEPVLYAGAFNLARTARLSGTTYNSTENESGVPTGIVGGGA
                     RYPSPIVLGILKDLGWTVHTQTLCEPLVDFYGYDAIGAVTLDWSLPETPYDIHTVTLY
                     LEGTEIVQLPASVLGYSEPALEGIYNFSATATYSMGVSEPVFTEVFVGPVDTDDPVAP
                     ELCLSRINVAPNPFRTSSLVSLELGRAADTRVELYNLKGQKILELQRAYLSEGVHSFT
                     LDAANLSSGLYLIRAQTGDQVINRRVLLLR"
     gene            complement(18050..18742)
                     /locus_tag="PLO35_00095"
     CDS             complement(18050..18742)
                     /locus_tag="PLO35_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78088.1"
                     /translation="MPKSRITLPVIETTVRWIITLLSLWAAYRYMLANDNVSLASAIL
                     CASLAQLAKPLQWLLRTRLPVSWRLVYLGFIIAAMYLGEIHSFFYRFLWWDDLLHTCS
                     AMMITYVAYLGFRLLYGKDMKSSGVRPILPAVCVFFFTMGFGALWELLEFGADQLLGV
                     NMLKGRDSTLPGSVYDYGRALINTMQDLGLDAVGALIVAIGAWFSLAREGRFSKAFGY
                     LIEQFIIANRSQ"
     gene            complement(19019..27331)
                     /locus_tag="PLO35_00100"
     CDS             complement(19019..27331)
                     /locus_tag="PLO35_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOH78089.1"
                     /translation="MKRIFVLSLLLLMISFAMADVYTIGTGTSASSTSPYYGLYDYSW
                     SKIIYTQAEISGAGLTAAANITGVGFYVGNTPSQYQMLDQRVYARHTTASLYETTDNT
                     YPGVTGYQQVFQGNLTYNGGGWHYIMFTTPFSWDGIQNIEFLFENWDTDYVTGYPTFR
                     YTSTTPNYLTVYKGQDNSFPNAVTGTRTYSRPNIQIVTPQTTPPDPAQAVYPTDGATL
                     VSPAVVLNWVPVNGFPNGYRLSLGTNNPPTNIVNNVDIQNVTSYDPDPDLQIGTTYYW
                     KIVPYNQFGDAANCPVWSFTTHGDSNVTTLPYLQHWDAVTAPALPFDWSAIIQSTSTS
                     AVVASYASTTYAHSQPNCLRLYNPSDADAILIAVGPNIAAPLTVNSIRVRFWARASGA
                     GYPLSIGVINDPQDPTSYTEVASIALTTTITEYAVNLTGYAGTGTKIAFKHGLGATGR
                     SLYVDDVTYEQIAQNDLACLAVTGNATPSVGNATTYSLAVKNWGTLPQDNYTVKLYNA
                     DNVELASAAGVSADPDATVMVPVTWTPTEQGPMALYGKVILPGDANPANDQSPNYNIS
                     VQPAGAVLVTVGEGNLAEGVPLEFYYRNSLHQALYFQNELNVYGNVTALTFYNNFVTD
                     LQNMPCKFWLKQTDLTDLSGGWVLDGLTLVYDGTINFPTGQNTITIPLQTPFTYTAGN
                     LLLYANRPWDTVYYSSSDNFQAQTLGTNRARKLTSDSTTYDPMAPSAAGTLSGTFAKT
                     SFTFVTAGFAELEGTVTSGGNPVADVDIVVNDTTYETATNASGAYSFPFLQPGNYTVT
                     ASKLGYETQTLPVTLIADQTAILNFNLVSSSTVTVSGTVYGSDQPTVGLADVEVSLSG
                     PLNYSGTTNAAGQFSIAGVLSGNSYNYLLVKESYQDLSGTITVGATSYDMGSLTMNEI
                     ALPPRQVVATENTAQTQVALTWRVPGGTGGGGVEDFEVDNGGWVPTSNWTNPLGDWQW
                     GNEYNAANYTDIDTYVDAPPAAAHSGTGMWGTVLQGGYTNATGWSYLKKTFNLSGYAN
                     PVLDFWHYMDGYNTWDYGLIKVNGTTVWGSSAAAEFMPWQRLTIDLSAYANQANAEIS
                     FEWYATGTVSYAGWYIDDLYVGAPMGRTVNYTYTPSQGFKSGGLESDGVASKQARSQR
                     TASQYQSDSRTREPQRVLTGYKVWRLTQGNETNETSWTLLTTNAIQDTFYVDSAWASL
                     PDGNYKWAIKGVYTNNVLSVPAFSNLIRILRLDLAALSISGTSTPSVGMVSNYTVEVE
                     NTGTTTQLGTAYTVKLMAGATELASVAGVTLEPGQTHSFVIPWNPSAPGPLAITGKVM
                     LPNDAVPANDETTPLNISVMPAGVYTVTVGEGTATEGRPVDFYYKNSLYQCMYYPAEL
                     GSYGSITALSFYNNFVTNLPDKPTKIWMGQTDLADLSGGWILPPNLTLVYDGNITYPS
                     GENTVTIPLQTPFNYMSGNLVLYANRPWEDVYYNTNDNFRVQTIGSNRARKLTSDTTT
                     YDPAAPSAAGTLSGQFPMTTFHMVASGTTPVFAVNPSSVNFGTVLINSNQSRQISVSN
                     AGGAPLVISSISVGGADAYTLQNLPAFPATVNFGQPLDFTLQYSPTAVGVHNGTITIV
                     DNMARQTHTIPLSANCINTTITALPYSQNFDGVTPPDLPVDWTKLVQATVTGADVNTY
                     ASTTYAHSQPNCVRLLNSTDANATVILEAPPLANTINTNTTRVKFWGRSGSATGYPLI
                     VGVMTDYQDASTFQEVQTLTLTSTYQEFVVTFAAYTGTGRVIAFKHGLGGTSRTFYLD
                     DVMIEVIPDNDLAATTIMGNSTPSVGMPATFTVGIFNWGTNPQNDYQVKLFGPGDVEL
                     ASAPGVQVNPGMAVDVPVTWTPTVEGASSVYGKVVLTGDQNPLNDQTGTFNILIQPAG
                     IMVFTVGDGSQNARTPIDMYYRNSVHQYLIFPAEIGNVMGQITGLGLNNQFVSNLQNL
                     PISVWIGTTPLTDLSAGWIPINGHTQVFNGTLNFPSGANVITIPFATPYMYLDGQNLV
                     LTFHRPMDTQYYNTADNFKAQTIGSNRCRKISSDSTTYDPMNMTAAGTLTGQFAQTAF
                     YIIPGGVGHINGTVLGAGNLPIEGAIVQFQTGGYGTTTNAQGQYSIQNVLPETYTINF
                     SAYGHINQSQTVTIEEDETEILNVTMQPMATVNVSGTVIASDTGAGLAGASIHLAGYA
                     NYDANTNAAGAFTIPSVYANQPYAYVIMCPGYTSVNGNIIVTGTNYSMGNVTLNEVAY
                     APHTVEAEEAVNGTSVDLSWQSPDPNAIEVTESFESETFPPQGWTQTITNTGPINTSG
                     VYPTWCRAGALTISGSPVNPTEGSYQAGLWWDYAHQDEWLITPAFNCPPSGYLYFDSY
                     VFLGSTNNDHYYVKVSPDNGNTWTVLWDASTQTGGWNYYASPITVDLAAYSGMQIKLA
                     FNATDGPNDDGLWYVWFIDDLYIGNALLPLGHPDGIVRFDMDQFETRSAGGQGFNFNP
                     VATTHPSRRMQEGSQRTEPSLPMPYQLKTHAASDRSLTGYKIWRLSAGQEVNEASWTL
                     LTPEQVTLLHHSDPGWQLLPNGNYRWAVKALYTADVASVPSFSNMLEKIVQTGLISGL
                     VRRQNTTPILGATVTAGGVSATTNSAGAYTLIVPVGTYDVTASATGFVTQTIEDVVVN
                     LNQTTTVNFIMVEGSPNADDLTPVTVTSLNGNYPNPFNPETVISYSIKDAGAVKLEIY
                     NLKGQLVKQLVNVDQAAGHYQVNWNGKDSSGRPCSSGVYYYRLSAPKYQQTRKMVLSQ
                     "
     gene            27777..28730
                     /locus_tag="PLO35_00105"
     CDS             27777..28730
                     /locus_tag="PLO35_00105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOH78090.1"
                     /translation="MPANIYVTRRIPQAAIDLLSSYFDVSVNPFERNLRRAELLEHIK
                     DADAILCLLSDSIDRSVMDAAPRLKCISNYAVGYNNIDVEYASKQGIVVCNTPKVLTE
                     TTADLTWALIMSCARRIVESDSFLRSGEFKGWEPMLLMGADIHHKTLGIIGMGRIGQA
                     VAKRASGFSMKVLAYDPSLPEDHNTALWKSTNLQTLLREADFVSLHVPLTEKTQHMIG
                     PGELKLMKRGAILINTSRGAVINESALIEALRQNRIAAAGLDVYEKEPYIPQELLELK
                     NVVLLPHIGSATTETRTAMGIMAAQNAIAVLKGEEPPSRIN"
     gene            28760..29599
                     /locus_tag="PLO35_00110"
     CDS             28760..29599
                     /locus_tag="PLO35_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH78091.1"
                     /translation="MSGIIFLRTRVLDQLTTFYTETLQMELWLDQGACQIFQKGNLLL
                     GFCQREEADLGGTITIFFPDRESVDQAYQRLGKIALAAPGLNPNYHIYHFYARDPEGR
                     SLEFQHFEHALNPYHTTVESLMTRRSIRSYSSEPVPEALLNRVFELCRYSPTARNLQA
                     YYYIVIKDPAIVDRIVELRGPAGEPLRAAPLTIAVAAKGEISRRVVQDACIAAYHLLL
                     AAHTYGLGTCWVTDMDNGPVKDLLGIPHEDYVACLTPLGWPAEHKPVPQRHEVSEFVR
                     YLS"
     gene            complement(29860..31380)
                     /locus_tag="PLO35_00115"
     CDS             complement(29860..31380)
                     /locus_tag="PLO35_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78092.1"
                     /translation="MKIFRALFIAVLVLWIGLISAQPQGELSQYGDKLVLKVWGSHYQ
                     RGYAQGYLLNQRIMNVFHGYYYTMFAFGSPTAYDYFWNWYQQHFSFHPRLYLEAEGLI
                     DGIAASGSSLWHDGLQRNLTPEDVMQVNAVVDMIQIRNQLRPESPLELGCASLSSWGI
                     STQQDSLLAGSAVITRFLDWSSNDALIANPLLVVHHPSENDEQKWMSFTFPGLLGALS
                     AINESGTAAFLNMGNDYNNPSPNNLTHVLFDIRDGLELIDADDDGVNKAEDVYHCLAQ
                     GHHYNGTIIHALDELEGNPNAMVIETSNTNTVLRRFNQYGNLPGNHLAATNHFRYGGS
                     TICCERYASIQDSLYANHQISAKRQWRVLSGAAGQTHNLSALQYTPSTGAILWTAATL
                     TDPAWSNSALSLDREELFNFNVSSSDELQGPVSLSLKLWPNPVSRGQKLNIEAANGLH
                     KVEIFNLRGQKVVSYNASGREASLLLDVDVPASGIYFLRVHDQRGASNTRRFLVIR"
     gene            complement(31445..31801)
                     /locus_tag="PLO35_00120"
     CDS             complement(31445..31801)
                     /locus_tag="PLO35_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOH78093.1"
                     /translation="MSTLYTLLNSTLLMLVLAIIFLLLNVLDGHSTWLVIRPRHFYRE
                     KNPIARWVFRKLGVPHGIVFFKAGLLAILILAGSFYGAWETFTLNIVLIVADLLFTYV
                     VIHNYRLYRTLRRWQL"
     gene            complement(31798..32772)
                     /locus_tag="PLO35_00125"
     CDS             complement(31798..32772)
                     /locus_tag="PLO35_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOH78094.1"
                     /translation="MLEDIDRWCAFLRLEGKSALTVKAYRSDLEQLREHLLELDPSSE
                     PRADNIGVIHIRSWLRWLHERPDGNRSLSRKTSALNTFFLWLKRDERIAVNPMDKIKR
                     PKYGRKLPNFFTEEEMEQLLRIPDMDNIFGIRNRAMMELLYSSGLRAAELAGIRMQDL
                     DLKRGVVRVTGKGDKQRIVPVGKSALEAIRAYLPVRQQLLSAHSGDILFLTRTGKALD
                     SRQIYVLLQGYIKLIANQKGFSPHTLRHSFATHLLERGADLRAIQEMLGHSNLSTTEI
                     YTHVTLEDVKKAYQTAHPRGQEPHPAERQTSDSARQNPIPDKEEHGKK"
     gene            32865..33620
                     /locus_tag="PLO35_00130"
     CDS             32865..33620
                     /locus_tag="PLO35_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOH78095.1"
                     /translation="MNRYVSDSAKLGQNVSLGINAVIMDDVVIGDNCLIGNNVVIHAG
                     SRIGEAVRIDDNTVIGKKPLSSPRSIFKVPSDLKPAVIGSFCQIGANVVIYCQCEIGQ
                     RNLIADLATVRENVSIGELNIIGRNVAIENYVSIGNRNKFETNAYITAYSKIGDYCFV
                     APGVITSNDNYMARDPERFNHFKGITMEDGARIGANATILPGKVLHTDACVAAGAVVT
                     RDAQAALIVVGNPAKAFRDVPEAQLIKNNLDKK"
     gene            33620..34336
                     /locus_tag="PLO35_00135"
     CDS             33620..34336
                     /locus_tag="PLO35_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOH78096.1"
                     /translation="MKAIVIIPTYNEIENIGRMLNQVLTEHPDLEILVIDDNSPDGTA
                     KDVKQIMATQTRVHLIERSGKLGLGSAYVTGFRYALANGFDYIMEMDADFSHNPDDLP
                     RLLEEAQKRDLVIGSRYCNGVNIINWPFRRLLISYFASKYVRVITGMPVKDPTGGFKC
                     FHRRVLESIDLDRILSDGYAFQIEMNFRAWVKGFRIKEIPIIFTERINGVSKMSRRIV
                     WEAAWMVWQLHFLKLLKMIK"
     gene            34347..34805
                     /locus_tag="PLO35_00140"
     CDS             34347..34805
                     /locus_tag="PLO35_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78097.1"
                     /translation="MKRLIPFILLLIFTLPLMAQPNLPRIYVQKLTLDNGKLPFVTWL
                     DKVSAPEYILEAWIKEKPGDLMSTETHSYHHLAVAQIGDGIKMPFTLVARVQLGNFKY
                     QWESGETLHLKLTHKATNTIKEWDVLVPEGSALIRYMDNPIVIPPYTESR"
     gene            34808..35824
                     /gene="ruvB"
                     /locus_tag="PLO35_00145"
     CDS             34808..35824
                     /gene="ruvB"
                     /locus_tag="PLO35_00145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOH78098.1"
                     /translation="MLERINDPIEHGPDQEFDRALRPKTLNEFIGQNHLKQLLDISIK
                     AAKLRKEPLDHVLFYGPPGLGKTTLASIIARELGVSITVSSGPVMEKPSDLAGILTNL
                     QRHEVLFIDEIHRLSHVIEEYIYPAIEDFEMEIILDSGPSARTLKIDIEPFTLIGATT
                     RAGLLTPPLRDRFGIVLRLDYYDFDSICQIIKRSARILDVRADDEGIHEMARRSRGTP
                     RIANRLLRRVRDYAQIMGDGIITLEIAQQALEMLQVDHAGLDEMDKRLLLTIMENYRG
                     GPVGIKTLATAIGEDAGTIEEIFEPYLVQQGFLDRTPQGRRATHKAYAHLGVKPQDSQ
                     TEIF"
     gene            35913..37181
                     /locus_tag="PLO35_00150"
     CDS             35913..37181
                     /locus_tag="PLO35_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOH78099.1"
                     /translation="MSYKANIASNLINQLLRLVLGLATSILVARALGPEGQGYVAYIV
                     LIFTLIGSYGHFGLNNAVMYFHKRKGIDASHLYHVNSTCLFIIFIALSGLTLGAWHNG
                     LWLRDYALVWVLGGLLFVLAEFYYNNHYAWFIASERIRESNRVNLLVLVLKSLFVMAF
                     WLLGTLGTGHWFWITVLALCLNAILLQIRLKRGFGFKWDTSLLKDEFAYGGIVFLAAA
                     FDHLHLRVDQLFIKNMLGIPQLGVYSMAVNLSELMFLIPLSINTALTGRLYNSEEGDS
                     GRMVTARTLKLTLYVCIILSIIGIPLSFAIPWIYGEGFRGAILPTIILLVGVIFASIA
                     RVAAPYFFTSGRPKVHLRVTLLSLLLNCLLNWLLIPLWGIRGAAIASAISYFFYGIYY
                     LSVLVVKEKFQLRDFLRLRMSDINAVLRKP"
     gene            37178..38797
                     /locus_tag="PLO35_00155"
     CDS             37178..38797
                     /locus_tag="PLO35_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HOH78100.1"
                     /translation="MNIIFATKRHPAWHYRGQVHTRGHAFSPDGSLHEGEDLCRKFSQ
                     VKSLSSFQKVLEGLDGSFCVIIAAANQIFAAVDRMGSMPLFWLENETHKLLTDDPEAA
                     LNAVPCKEVDHLSLTEYLLTSYATGPNTLQKGLYILPAGQILHYARETDQISRSEYYR
                     YEHVSAELGTDAELFKQLDLIHQNCVKRLIQSADGRPIAIPLSGGYDSRMVALMLKRL
                     GYKNVSAFCYGDPRSNETSISKAVAQYLEIPWTFVEHRPRDWYLAYQSPLRQDFYRFA
                     GHFSSRPHIQDWLAVRDLQHRNHWHPETIFVPGHSGDFIEGSYIPPAFVERNTVSKDL
                     FLDHAIQRHYRQWPYDPRAGEQANAIRTKIAEQLKLPEKMSSEEAASHFETFYWLEQQ
                     PKFILNSMRVYEFFGLEWRLPWWDKELLEYWKRIPMAKRAGREFYKRYVSALRDTGFP
                     VYKKESLIRRMEDRVIRQRWGYLYESRWSRFADLTKDKESRSTTVQGLLPQGLDLPAF
                     VNPKQRIVDTDINGLQSLVAIKEWWEEGHRV"
     gene            complement(38937..39857)
                     /locus_tag="PLO35_00160"
     CDS             complement(38937..39857)
                     /locus_tag="PLO35_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78101.1"
                     /translation="MLRTALLTALLSVIGCMHAETMVRSYPVPNPYLPVTWQSHDLKG
                     RVKSYTIRTDGNPDEYQTREYTEDGKLLRSVLYSAGHIYETSFWYDAQGWMIKSLANT
                     SIDGVASEPEVILYQYEFDRQGYPLIVKELSASGEALVTHSYEYADSVYSLISQNLQD
                     SEEDSYITVYLFNSRGEVTARTVFDISKGHSERYTYAYIPDQSITIESSGYQEGVAQE
                     KVVTRKNNLGKVLERSVYDAEEHLISKLECEYDPDGKLITESWCFPAYQSTDVKSYAY
                     ELDSTGNILVQRSHSDGEDDEHKMFSYEYY"
     gene            40249..40665
                     /locus_tag="PLO35_00165"
     CDS             40249..40665
                     /locus_tag="PLO35_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78102.1"
                     /translation="MKKAMMTLVFIMLLVGLGAQYVPVEIVNQAEDRNGKALVSKFRE
                     IIRNSSAYQISYDSESPHFVVQINTMDRYKGDSEWEGVSTIYHYMILIDPGDQMRVLC
                     TNQLGYVGANLLDQVAYGIYSDLDDFVEMVRYYIEN"
     gene            40717..41667
                     /locus_tag="PLO35_00170"
     CDS             40717..41667
                     /locus_tag="PLO35_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78103.1"
                     /translation="MKQLLFLLAALLLVSGCAQTRLVSWETRKVDVPGQKNNILDLAT
                     GTVNTAIQSREYLLLYNAVYNHGIPANILAKINKNDRVAVLAAERDTLEYADFMHLFV
                     NAMNRNLINAGYQVVDLRPVSGKFGLEPERLFASIDKLLIFTIWEAGSRSVIFDEKAS
                     VFSGFQMSLDVVEAKNNQFLASSPVFGSARNDFDKNEFDRFQLFTLSEVNASLPLIYA
                     DTSKKGVIALADSPQGRVFMLTVYNPEQMKLMMKITDISGNNIIEKEISFGDSSSGGY
                     YSYVWDGKLIDGTDIQAGDYLLYIKSADGVTIGYRSFRVD"
     gene            complement(41669..>42690)
                     /locus_tag="PLO35_00175"
     CDS             complement(41669..>42690)
                     /locus_tag="PLO35_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOH78104.1"
                     /translation="DEFLKRCGSYARFLKPALYKLSPAKLIGYLNSLGCQSIVRPDGK
                     VFPASLDAKDVLAALLRGALKPGNQIISSARISHVSIDKGFVLTDNHQRLYRCRKLLI
                     ATGGASWPQTGSDGSGYELARQLGHKLIEPRPALASILIDAYPFRTCAGIALNGIKAE
                     LLTQAGKHRVSGDLLFTHRGLSGPLVLDHSHLLAQGDTIRLCYLEDAAVKLRQIMASR
                     PKSSLKNALKTTGLPEALLLVLITMAKVNPDKRCADVSAPELKSLGFSLSSSDLKLKS
                     VECLSTAMLSAGGIDLGEVRARDMSSCLVPGLYFAGEVLDYNLPTGGFNIQAACSTGF
                     LAGSQ"
     assembly_gap    42691..42790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<42791..42987)
                     /locus_tag="PLO35_00180"
     CDS             complement(<42791..42987)
                     /locus_tag="PLO35_00180"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOH78105.1"
                     /translation="MIGAGPAGLMAASASTGTCLILEAGPRPGRKLLLSGSGQCNFTH
                     DLELDEFLKRCGSYARFLKPAL"
     gene            complement(43008..43751)
                     /locus_tag="PLO35_00185"
     CDS             complement(43008..43751)
                     /locus_tag="PLO35_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOH78106.1"
                     /translation="MKKALYIFCLLVFSALLCAQKAPSAGSILKAVDKNMLSSTSKST
                     TRMVIHSKRSSRTVESINYSSGSDKYYSEYTAPPREKGTKMLKVGDKLWIYEPSSDRT
                     IQISGNMLKQSVMGSDLSYEDFMEETKLEESYTATVTGDSKYDGREVWVLELVAKQKN
                     LAYHKLKLYVDKERYVVLYQYMYAKSGKLLKTIKAGNVGRVGSRWYPRYILFKDELKD
                     GKGTEYYVDAIAFDLKIPAATFTKAMLKK"
     gene            complement(43748..45016)
                     /locus_tag="PLO35_00190"
     CDS             complement(43748..45016)
                     /locus_tag="PLO35_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78107.1"
                     /translation="MLKFILKGIFRDRHRYLFPLIIVASGIAILVFGLAFMEGFTVSF
                     VRHNARFETGHLKVVTKAYAKQLSLKPFDLAMLDVEPELARWKQAYPQLDWVQRISFG
                     ALLDVPDSLGETREQGEVAGFAADIINNPAERDRLNLNKALVDGRLPRQKGEVLISRT
                     AFHKLRLRLGDPITLISSTVHGAMSMHNLKVVGSVDFGVHTLDRGAVVADISDVRTML
                     DLEGGASEVLAFFKHGNYKASEVNKLKADFNSRFSGPEEFDVEMLALTDQGNLAYFMA
                     IMKSSLGIMSLVFIIILGIVLWNSGLMNGIRRWGEFGLRLAVGERKIHAYGSLVGEAL
                     VLGIAGSLLGILAGGAISLLFARNGIDVSAYARSSSIVSESMIYTALNLQTLLIGLIT
                     GVMSTLVGAALAGIPIFRRQTSQLFKELES"
     gene            complement(45018..46193)
                     /locus_tag="PLO35_00195"
     CDS             complement(45018..46193)
                     /locus_tag="PLO35_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH78108.1"
                     /translation="MIFRLALRNIIGNGWRSLINVFIIAIVMIGMIFMESMYHSWTLL
                     AKTQQKEWEFGKGMFRVNSYDPFDAFSWDKSFEPLPDELRPVIERQEAVPILFSPGVI
                     YPGGRMIPCMVKGIPADQTLLNIPSSRLVERTPGYTPAIIGQTMAKSSQLEEGDILTL
                     RIKDVGNAFNTLDLEIVHIMNTPVPTVDGGIIWMDLSTLQELKQAPSMVTAIALQDPS
                     LGVLSLPGFHYMDEKALFFDLDEMMKTETVQKYITYSLLMFLAMIAIFDTQALAVFKR
                     RKEIGTLTALGMSQRQITTMFTLEGGLYSLFAIGMTAILGTPMFWYFATTGYIMPEGY
                     DSFGILGFMEPIKMSYPPLMLLQVFSFVVLVSVVVSWIPARRIARMKATDALRGKVV"
     gene            complement(46171..46869)
                     /locus_tag="PLO35_00200"
     CDS             complement(46171..46869)
                     /locus_tag="PLO35_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH78109.1"
                     /translation="MQELIVTVKGMSKAFPTGEGEFLALKNIDLEFGKGEFTGIVGPS
                     GSGKTTLLNVIGSLDVPSSGEVEVLGHKVSKLSARQSAELRSQHIGFIFQTYNLLPVY
                     NVFENVEFPLLLLKLNSEERRKLVNEALEWVGLTDKIKSRPAQLSGGQCQRVAIARAM
                     VKKPSLVLADEPTANLDTENSLHILKTMREINLKTGTSFIFSTHDQKVIDFLTRVIKL
                     VDGRVAADDLQTGA"
     gene            complement(46945..48018)
                     /locus_tag="PLO35_00205"
     CDS             complement(46945..48018)
                     /locus_tag="PLO35_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78110.1"
                     /translation="MRLRATCVCLLVLALSRIGAEDRFELKNHFSLSMERDQKHQDSW
                     LELNWQPQLGYSYPLLDKLELSGEYSMQNRLLSNWQEGSYTAEIKLDSYRWWLRLATD
                     QTQVLAGLQRLNFGSAMILRPLRWFDTLDPLDANQETQGVKALLIRHNWLNNANLWLW
                     AMPGSDDLKGMELLSGRDDRIELGGRLQLPSRWGETAISYHQRELIQGREYRLGLDHR
                     LDSFMGLWLESSGSLYENVSHGFERYQLSATLGGDYTVGIANGLAFTLEHMILAGSND
                     PESLHNQASVSALMANYPLGLLDGVTILGSWHWTDESGNLSALYRRAYDYLSFDLGLA
                     LDTGMPDQLSRSPYLYLKVSYDI"
     gene            complement(48142..48642)
                     /locus_tag="PLO35_00210"
     CDS             complement(48142..48642)
                     /locus_tag="PLO35_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78111.1"
                     /translation="MRKSRIFACELKSYREVTPPLCAVCPRIERCRSFRAWYRSHKKE
                     YVDFVVDIVRKFPDKYQMEVSFMAEKQQFVQIVDMATGAIERVTTLAEIESLSAEDKL
                     ALSRNKNLFVVTHRLEPIVKVELRRHPITEPMQFSSPETKEESSPIEEITPDPLKPSK
                     ARGRKK"
     gene            48836..49741
                     /locus_tag="PLO35_00215"
     CDS             48836..49741
                     /locus_tag="PLO35_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH78112.1"
                     /translation="MLLSDFLDPRTILMESRILTREQVYENMMRKLCETHKHDLPICG
                     MPLLEKILERDREASTAYPSGIAIPHIRLDGFQDTLISLCFMQNPLDFDGTMVSWVAL
                     IITDKSSSRLYLNIVAELLGLSKNQALMERLLRERDGVGIKHVIAKEGIRVSKDIFLS
                     DIMISDPVVVHPDTTLKELNALMNTHNIAGFPVVDEKGRFKGEVNILNLLKVGVPDYL
                     LMLENLAFLQSYEPLEKIFEQEESILVKDLMDKDVITLNSNASIIEAVYEMILHKKRY
                     FSVVDQGQLKGVITAMDIFRKVIKA"
     gene            49756..51027
                     /locus_tag="PLO35_00220"
     CDS             49756..51027
                     /locus_tag="PLO35_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOH78113.1"
                     /translation="MLLALVIFVLTYVLIITEWMNKMLAAMIGGFMIVVTGIMHQKEA
                     LMAVDWNVIFFLIGMMFVISVLKKTGVFMYMAIRTAKIAKGKPLWIMILMFLITAFLS
                     AILGSVTTIMILVPIVLLICGELKIAPIPFIITMVIASNMGGAATMVGDPPNILIGSS
                     TSYQFLDFLFNLTPPVMLITASSIGIIWLMYRHKMRVSNENRARLMSYREDSLIKDRP
                     LLIRSGIVLLLMLTALAMQGTLGVETATISMSAGLLLLILSDRKKVEHVLMNDVDWVT
                     IFFFIGLFMIVESLVKTGFIGMAAKQIIIATHGEPKAASMMILWLSGVFSAFIDNVPF
                     VAAMIPMVKTMGTMINNPSAMHPIWWSLALGTCLGGNGTLIGASANIVAVGIANRNGF
                     HISFKQFTIIGMIFTLNSLLISAIYILLRYF"
     gene            complement(51315..52295)
                     /locus_tag="PLO35_00225"
     CDS             complement(51315..52295)
                     /locus_tag="PLO35_00225"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOH78114.1"
                     /translation="MLFIVSDSHHAPFNIATEEYILHHIPEDVFLLYINAPSIIVGKH
                     QNTLAEVNLDYVREHHIPVVRRLTGGGTVFHDLGNLNFSFIVTDDSDKLHTFEKYTRP
                     ILEVLNRLGVEARLEGRNDLTIKGMKFSGNAKAIIAGRILQHGTILFASRINDLSKAL
                     KANPLKFRDKAVKSIRSRVTNVKEHLSQALSLEEFITLVHARIREIYPDSSDYRFRPE
                     QLAAIQALSKAKYESWDWNFGHSPAYSKHRGIRTAAGTIEVYLEVKHGRITGIRFYGD
                     YFGNKDSTELERAMLNQPHQEQILREVLSSHQLSDYFGLVEVDDILGMLF"
     gene            complement(52306..54021)
                     /locus_tag="PLO35_00230"
     CDS             complement(52306..54021)
                     /locus_tag="PLO35_00230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOH78115.1"
                     /translation="MNSTHILTPKTPKYLPAGFEVRDPSTVLGSLEALAASVPSSAQD
                     LITVLEHYSEISMAVEDELSRLYVEMTVHADDESKEQAYNDYYAQVAMPSEPFSFRIK
                     KLFYDSPWRAELSQERFGHLNRMLAKQIELFREENIPLKIQESELANKYGGIVSKMTA
                     EFEGKELTMAQLGVYFKDADRQKRETAWRLRMQAFQDKEAEFDTLYDQLRKLREQIAA
                     NTGFANYRDYMHEWMGRFSYTPDDLYRFHDAVAKVVIPFTNQLSEERRQRLGLESVKP
                     WDTIVDLDGKVLKPFETTDEFIDKALQVLEKVKPDYATQLARMRATGLLDLENRKGKA
                     PGGYNTTLNDLGASFIFMNHVKQHNDVVTLLHESGHAMHSAATKDIPICFYKDTPSEV
                     AELASMSMELLSMDHWDIYYQDRDDLIKAKRDQLEGTLSFLPWCMSVDAFQHWVYTHP
                     EHDVDQRRAAFLDIYNRFFPDVDWSGVEGYKQLRWMQQLHIFEVPFYYIEYGMAQLGA
                     LSVYMNYREDKDKALKAYQSFLELGYSKPVSELYQAAGIEFDFSEQRLSKLVDFVKRE
                     LDSLK"
     gene            complement(54022..54444)
                     /locus_tag="PLO35_00235"
     CDS             complement(54022..54444)
                     /locus_tag="PLO35_00235"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOH78116.1"
                     /translation="MKFLEILSQVRSALSKGAGADLSFGKATQLNDVTVIPVARISMA
                     FGGGGGSSPVTAKKASKKLETQTSESLEESPEAKSQVNEGGGGGGQISTTPVGIYTIK
                     GDKVKFYPLVGVREILLTSGLVSILWIKIIKLRRYKRK"
     gene            complement(54513..55460)
                     /locus_tag="PLO35_00240"
     CDS             complement(54513..55460)
                     /locus_tag="PLO35_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOH78117.1"
                     /translation="MVKYLVKLPYLALLIILLFSLSACEFTSKPRFEGEVYTVAGLLV
                     AGKSISTENPIFVTRSISLEDFDPFELFVEDAEVTVIDLQTNERWELEEALDPSRGFI
                     YVDPQNRIIQPLQSYRLEVRIDGKPELIWAETTVPQAVTPVMDFYQNNPPGTGWSLDP
                     ETANTVQYSILDQQYPIVLGTGAQAGDFNFMAEIYCREPFSTDLEWTTPIFGITNLDE
                     SLEPIYNSNGQSFRRINVMGIYTSKSQPGMDGNYLVLPDYAASVAFYGRYRVSAYIID
                     DNYYEYSYRPEGYFYGGINNALGYFGSASGADFYARVVK"
     gene            complement(55454..57778)
                     /locus_tag="PLO35_00245"
     CDS             complement(55454..57778)
                     /locus_tag="PLO35_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH78118.1"
                     /translation="MVRLSMITAILLLVSGLFSATVSGFVTREGSDEPLQYVNVSVGG
                     TRIGGQTNKKGYYVLTLDKPGSYTLEISLVSYKSQSMPFTVKSVGENVTLNVRLERGS
                     IELQKTVVYGTAEAKYEGPEIATSLIRQTTEDIKSIVSPVESDIFRAVLALPGVAPIS
                     DFSAGLYVRGGSPDQNLILLDDIDVYNPNHFGGIFSTFNTDAVESVDLIKGGYPAKFG
                     GRLSSVLDVNNRQGNRLRHQGVARLSLISSSATLEGPWKVAGQNGSYMASFRRTYVDL
                     VQKFYEELPEYYFYDGHVKLNWDMGQRDKFSASAYFGKDDLSFDFGSKLALDWGNNTF
                     TTQWIHLFNPTLFSQFILAGSEFTSHFDQVDLQGEKTFTRENGIQDLSAKAMLSWKPN
                     NQHEVDAGAEIKWNDTWLKLNTSYQLSQNSLPDVEVASLTSSAYIQDIWDINTFWTLQ
                     PGLRLAWYRSLRTKPSHIPAASYFNVEPRVSVRRRLDVGEEVYAHFGAYHQYLTLMSM
                     DVSTPFDVWFPLDGSLPPGLSLHYILGYKKEISSNFALDAEVYYKSYDNILQYNTATD
                     FTWDNETGNLADTFHVGKGYTYGLDLLVRNDWKGLQGFTGYTYGQTRRRFPEVNIDPA
                     TGEPRYFYPKYDRTHSLTIAQTFNVSANTGWQLLGADLKLGMNFSYNSGQPEYVPERI
                     YYDGSNFQIIYSYQDRVRLPSYLRLDLSTKYEFVTRWGSIEPYLEVINVFNRKNVAFR
                     NYRLVTEDDGGISLLESDSAQFPLLPFIGVNIKW"
     gene            complement(57997..59586)
                     /locus_tag="PLO35_00250"
     CDS             complement(57997..59586)
                     /locus_tag="PLO35_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH78119.1"
                     /translation="MKKTQLSLFRPFLMLAFVLLMLAACKDNTTNPGDQSVAACEFSP
                     AGGVYDSSVDVLISCPTSGVSIRYTTDGSEPNSASPLYAGPVHLAVSSTLKAKAFKQG
                     WISSSTASADYVLTSIAPPQIDPPGGSYTSAQTVTVTCATAGAEIRYTLDGSDPNENS
                     SLYTAPLTVAASMVMKARAFLAGKNPSEVTQAVFTMRLPDPIFSPAGGYYSTAQMVKI
                     SSGRRNVRIHYTTDGSAVTEASPLYTYPINVPVNTVIKAKAYQDGWEPSLEVQEFYII
                     NLADQMQLVPGGSFHNGSSNVTLSPYYIGKREVTELEWVMVMVDMPEIDQELPMTTHD
                     VEGVTYTMNWGKAVIYCNLRSILEGFTPCYNYNNLGVNPASWPDGWDDSVNHELLACD
                     WNANGYRLPTEMEWMYAARGGSYSQDLIYSGSNDLDEVGWYVENGGDGVKPAGTKMPN
                     QLGLYDMSGNLWEYCWDIYHNQYPAADVTDPTGPASGPHRVMRGGSWNSDASNCTVAR
                     RFYSLAALSTNFTGFRVARKA"
     gene            complement(59583..60440)
                     /locus_tag="PLO35_00255"
     CDS             complement(59583..60440)
                     /locus_tag="PLO35_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH78120.1"
                     /translation="MRKLAVICIIASLTVGGLWSLDLDWGIKYGFGSSKLTGDDRGYE
                     LGYDLETVGVVNAPLGYLTLNSRKTESGAAQNLGLWGCVNLVKKTDSVGLQAELIWER
                     FRYEHLFEGRSLSTNNLLLATEFADTLEGSIHRTVDYITLPILFRLQQELTQEQKGGQ
                     YQGAFIYLGPSFGIVLSNTSSSEGGIKALETDIEDFVLASQTDADVANSYLSQRRESG
                     SEQLLTYKTDLVVGLGFGLRDIFQLGLGKDGFVFDLRYTAGLHDLGKANLRKAFRMRS
                     IVVSVGVLL"
     gene            60700..60957
                     /gene="purS"
                     /locus_tag="PLO35_00260"
     CDS             60700..60957
                     /gene="purS"
                     /locus_tag="PLO35_00260"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOH78121.1"
                     /translation="MLKAKIYVRLKPSVLDPQGKAVTGSLTQLGFDTVIDTRVSKYIE
                     ITFSDDDPSIVKPKVEKICDTLLANPNTEHYSFELEKVDPA"
     gene            60954..61649
                     /gene="purQ"
                     /locus_tag="PLO35_00265"
     CDS             60954..61649
                     /gene="purQ"
                     /locus_tag="PLO35_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOH78122.1"
                     /translation="MRVSVVTFPGSNCDHDARDVFRLRGHDSRLIWHKDRDLQDPDLV
                     VLPGGFSYGDYLRCGALARFSPIVEEVISFAKRGGLVIGICNGFQVLTESGLLPGALM
                     MNSGLDFICQHQLLRVESSSSPWTHGIPTGTVLDIPIAHKEGNYFIDADGLKALEDAD
                     RVLFRYCGPDGSITAAANPNGSLNNIAGICSPNRRIMGMMPHPERAASPEVTSQDGGK
                     IFEALERYLDSLG"
     gene            61621..62322
                     /locus_tag="PLO35_00270"
     CDS             61621..62322
                     /locus_tag="PLO35_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOH78123.1"
                     /translation="MNAILTPLADLLLTPLCFGCGSRLREASDLICEDCLAQLALPGE
                     DTCKLCGTQLEQGACPHCAESIPLFRVARSAFLFKSPVRELVHAFKYDSYTRVSPWLI
                     KGMLQTYQSEPAFSCCDLVTAVPLHWVRQRDRGFNQSELLARQLAKHLGLAYKQLARR
                     SHHTPSQTRLSKEERQHNLGNAFKLKSKQDLSGSHILLIDDVFTTGTTVNQLSRLFLD
                     HQAASVAVLTAARAI"
     gene            62319..62729
                     /locus_tag="PLO35_00275"
     CDS             62319..62729
                     /locus_tag="PLO35_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78124.1"
                     /translation="MNYKKHPYDDVHTQIDPAEAAEMIEKVARFIAERHFAAAGIMLV
                     ESLRPLHSIGSQALFFILPFAEILFDSRKYQQFALMIQNEDNLKKLVARMDELDEEIN
                     RERRIAAKLKRKRRRAKTRQFIHKIFKIKDNKAE"
     gene            62741..64603
                     /locus_tag="PLO35_00280"
     CDS             62741..64603
                     /locus_tag="PLO35_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOH78125.1"
                     /translation="MQYDEKRLTRIVATDCGSTTTKAILIEWVDGEYRQTIRGEAPTT
                     VEAPLNDVTKGVINATQELEELARLKYKNDNIKFMENGEFVIPRKGDVGVDAYVSTSS
                     AGGGLQMMVTGVVASMTGESAERAALGAGAIVMDLIASNDKRMNHEKIERIRQLRPDM
                     ILMAGGEDGGTIKHVVEMAELVSGADPKPRLGSGYKLPVIYAGNKEAQKDIQDALKDK
                     VDLIMTDNIRPKLETENLGPARDKIHDLFMEHVMKQAPGYNKLMSWTKGPDHEEVPIM
                     PTPAAVGNIMQTIAKDEQIEVVGVDIGGATTDVFSVFTEEYVFNRTVSANLGMSYSIS
                     NVLASAGLDNIMRWVPFDINESELRNMIKNKMIRPTTIPSLLEELVLEQAIAKEALRL
                     AFEQHKQFASSLKGMQRQRDISEAFSQSTSGATIVNLMTLNLLVGSGGVLSHAPRRNQ
                     TVMMLIDAFLPEGITRLAVDSIFMMPHLGVLSEICSKAATEVFRKDCMVYLGTCVAPV
                     GKGKLGKPSLYAKLELPGGKVFEEDIPYGEVRLIPLGVGEVAKAILKTSGGLILDKEK
                     NKELQTEFHGGVVGIVLDTRGRQPFGLPTEKTQRIEYLKKWMRELDAYPQEILV"
     gene            64613..65743
                     /locus_tag="PLO35_00285"
     CDS             64613..65743
                     /locus_tag="PLO35_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78126.1"
                     /translation="MGQAYSAGLTVTDSIILRKERLLPLKGTVLVKKGDKVKAEDVVA
                     ETLLPGKVVPFNLANKLGVTPSQLNNFIKIQPGQQITKNTILAENKGLFGLGIFKSEV
                     RSPVEGEVESISSVTGQILLREPRIPVQVKAFLDGIVTEVIPNEGIIIENKSAYIQGI
                     FGVGDETVGLIKMLAETPDDELEIGKIDESCRDKVIVAGAFVSYHVIDAARKHGVKAI
                     ITGGIDDQDIKKLLGYDIGVAITGHEKIGLTIVCTEGFGKITMAQKTFDLLKQFDGHK
                     TSIHGRTQIRAGVIRPEIIIPQQFDENELIVQEASLPILEPGTLIRVIRQPHFGRIAK
                     VTRLPEELTKVESETLVRILEAEFEDGTKFSAPRANVEVIDS"
     gene            complement(66488..68599)
                     /locus_tag="PLO35_00290"
     CDS             complement(66488..68599)
                     /locus_tag="PLO35_00290"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOH78127.1"
                     /translation="MVLRFTLAVNSSLSGTLTAVTGSYIGMLFTQAIPNPTTPAPLLG
                     SATNGGTVATLTNLELAAGTYYLILSTWPAPQSFTFSLDLTATPLATDPVFQINPTAW
                     DYGQVMPGNSVAKVFTITNTGGGTLFIDMDDVTITGTHADQFSLEALAADISLVRNAT
                     AQITVNFGPESEGIMTANLQIIDNIAETKASQRSGAKSTHIIQLDGEGYDPSVSSFPY
                     AESFDGSTFAPAGWINQQISGPGEGLWERTLVGTNPDCLPHSGEAMAEFNCYNYNDGT
                     SGMLVTPPLSFGDADYQVSFWMYRDSGYSSESDQLNVYAGAITNINAATWLGTVNRHL
                     DLEPVVDEAGWYKYSFPIPEILMKGVGYIFFEGVSDWGNNIFIDDVSIEEASQGVIPI
                     DPGNPGMPPNGVEFTPGGPVPPALLGEDSGLPALIYTISATGVWDVLVLKPMDWNTDW
                     YCWIMAGTQLLAGPNPIPSTTPDYTFAGVNFDAKGDVTVVVNDNQTLPVELSSFTAVL
                     TADMFVRIAWMTESETNHLGYNILRGSNDSVSLAQRLNASIIDEGTGLGSQVSYDFTD
                     AEVDAGSTYYYWLESVDLGGLSTLFGPLTITVSGQPNDPGAPEIPSVTQLQTAFPNPF
                     NPSTSLRYSLKEAGKVRLEIYNLKGQLIRRFEHDHQHPGYYQQVWDGKDAQGNNVTSG
                     VYIYRMVTGKYSSSRKMVLSK"
     gene            68678..>69789
                     /locus_tag="PLO35_00295"
     CDS             join(68678..69104,69104..>69789)
                     /locus_tag="PLO35_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611411.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOH78128.1"
                     /translation="MIKYKITLSKDERAELEALVDKGIHHVSRFKDALILLNCDEGIN
                     ARKYPNRVISDVAKVSMKTIDRVKKKFVEEGFDAVMYPGKSKRVYVKKFDGELEAHLV
                     ALCCSEAPSGYAKWSLRLLADKMVELQYVDEISHESVRQIPKKNELKPWKSIGWVIPP
                     KQSSEFVARMEVVLDVYKRPYNKDNPVICMDETPKQLIGETRIPIPHSPGTPAKHDYE
                     YKRNGVCNIFMASEPLNGKRYVQVTEYKKKDDWAIFMKSIADKYKDARKITLVMDNYA
                     THTMGSFYEVFPPEVAKALIDRFEFIYTPKHGSWLNMAEIELNVMQKQCLNRRIPDIA
                     ILQREVNAWTRERNSKKCQINWQFTTQDSRTKLKRLY"
BASE COUNT        16024 a        18283 c        18881 g        16488 t
ORIGIN      
        1 tggctgggca gggaggattc gaacccccgg atggcgggac caaaacccgc tgccttaccg
       61 cttggctact gcccatcaaa gctttctggc ggagagggag ggattcgaac cctcggtagg
      121 cttttgacct acacacgcnn nnnnnnnnnn ngaacccggc ggttttcaag accgctgcct
      181 taccaattag gactaatcct ccacagcaag tttaatcctt caattctaag gccgtttttt
      241 tgtcaatcga tttcttgggt aagcgctccg attagcctcc catgcctatc gcacgcttat
      301 cgcacgccta acccgtgctt tggcctgggt ggagggtgag tagtccatta gatacagaca
      361 ggagaccaaa caccaaggcc agggtaatag gccctgaatt gactataggg atggaactta
      421 agtaatcagg ttgtcatcgc tttgatgcgg ttcagctcag cttcaaagcc ctttttgccc
      481 atcagggcat aggtggcgat tttgccggct tccacaccag gctgatcgag aggatcaatg
      541 tgcagcaact tgccggtaaa gacggtctgg atctcataca tcatgataaa ctcacccagg
      601 tgatactcat ccagacgagg gaagccgatc gtggcattgg gccgtcctgc cgaagtgagg
      661 gccaccgcgg tggcgagggc ttcggcattg agcagttcgg agagcttgcg gccgcccaga
      721 tagctcactt cttcccgttc gggatgcaga ttggggatca cgtagtcgtg ggcaaactca
      781 tccacagtga ggaaggtaaa gactttatcc atcggacctt cggcataaag ctgcacctga
      841 gagtgctgat cggtggtccc cagcgctttc acaggtgtct ggcccacaaa gacttcccgg
      901 ccgcgggtgt cgcgccgttt gccgagactc tcagcccaga gctgacgata ccagtccgcc
      961 atatcgtaga gcgcgttgga atatggcatc atgacgctga tgtttttgcc ttcccgcata
     1021 tagagatagt gcagcaggcc attgagatag gcgggattgc tgtagatatc agtgtcttca
     1081 cagcgtgacc gcatggcctt ggccccttcc agcagcttgg cgatgtccat ccccgcaaag
     1141 gctgaggata gcagaccgac atccgtgagc acggaaaagc ggcctcccac attgtctggc
     1201 accacgaaag agggatagcc ctccttcctg atcacctgac gcaagaaacc cttttccctg
     1261 tccgtggtga ccacgatccg cttcttatag tcctggggga atcgcttttg gacgatatcg
     1321 gccaggatca tgtagccagc catggtctca gcggtggtgc cgcttttggt gatcacgttg
     1381 aagagggtgc gatccagatc gatctggtcc agaatctctt tgaggaagac gggatccacg
     1441 ttgtcataga ccaatacgcg tctgggaagc tcgcgctccg ttttcagggc agaatacaaa
     1501 gctttgttgc ccagagcaga acctccgatc cccagcacca ccatggtgtc gaaccggggg
     1561 tccaagccag ccacaaaatc ggtgatgtgg ctaacattct gttccggtag ggcatagaag
     1621 cccaggatgt cggcctggcg gtctttgccg atctcgtcgt gggcatccag gatggcggag
     1681 ctcatatcgg ctatccgctc cggggcgata gctgtaggga tgtgttttga gtgcagtaaa
     1741 ttacgatatt caaacctgat catggggcct cctcagtcct gatagtatct tttcaggtca
     1801 tggttgatta gttgttccac cgcggccaga tcttgtcttt cgcttgtgcc gaggattgat
     1861 gtcagttcag aatcacagcg gcggatgatg ttcttgcaaa aagctacccg cttgggatgg
     1921 acgctcaatt catcgattcc catgcctacc agcaggggaa tgtagtgcgg gatggaaccc
     1981 atctctccgc agacgctaac agctatgcca tgcttgtggg ccgcggaaat gcttaaagca
     2041 atgagtttta gcactgccgg atgatgttgg gtatacctgc cgctgagggc atctgagttg
     2101 cggtccaccg ccaaagtgta ttgggccaga tcgttggtgc cgatactgaa gaaatcggca
     2161 tatcgggcca gttcgtcagc acaaagggcc gctgagggga cctcaatcat aactcccatg
     2221 gggatatcgg ccctgcaggg gataccgcgt tcacgcagct ccgcggcgca ctttttcacg
     2281 atctctttgg cagaaatcag gtcactggca tcgtttacca taggaaacat caggctcaat
     2341 tcgccatgca cggcagccct cagcgcggct ttgagctgag tcttgaacac atctggccgg
     2401 aacagcgaaa acctcagacc ccggcatccc agataagggt tatcctcccg ctccgatgga
     2461 atcagatgag acagcttgtc accccccaga tcaaagctgc ggatggtgac gggataaggg
     2521 gccatcgctt ccagcacctg agtgtaaatc tgatactgct catcctcgct gggcagagcg
     2581 ctacggtcca gatagaggaa ttccgtcctg aacaatccta tcccctccgc tcccaaagcc
     2641 cgaagctgag aggcttcagc gggaagctcg atattgcctt tgactctgat cattttacca
     2701 gtctgggtca ccgccggcag gagcgcgtcg gccagatctt tctgtctggc agtgcggaac
     2761 ttggcctgca actcactgaa acgcaagagg gtatcttcat ccggatcaat gatcagctga
     2821 gctcccagag cgtccacgat caccgtttgt ccgttatcaa gctgctctga gagatcggcg
     2881 atggcagaaa gcgccacgat gtccagtccc cgggtgagaa tcgcggcatg ggaggtgtag
     2941 gagccctttt ggctcaccca agcgcctatt cccttgctgg ccatggcggt tacgagactg
     3001 ggactgggct ctgggcaaaa gagaacttca tcggaggaaa acaccgccag cggatcggca
     3061 gaatggccca acaggatggc caggaggcgt tcctgcaggt ctctgtagtc cgcagcgcgc
     3121 tgggcaaaga aatcgttttg catggcggta aacatggacg caacttcatc aaaggcagtt
     3181 ttcacagcct gggccgcgct gttgtaatcg atcagcacca ggccttccag cttggccagc
     3241 aggtccggat cgcgcaggat aatctgatgc gtctcaaaga tttcacgctc gctgcctgtt
     3301 ttggggaggg cgtccagcag ttcatccagt tggaggacgg cggtttcgat agcttgaagc
     3361 aggccctgaa actcctgctc agcattttcc gggttaatat ggctgtgaag cactaccggg
     3421 gaacgggaac tgaggaatcg ggccctgccg ataaagatcc cggggttgat ggaattgccg
     3481 gtgaggatac gcatgtcagc gagactccgt actacttgtt gaaactactg agctgcagag
     3541 gtttcctttt ttcactacag agctacagag gaaacagaga gaggtacaga gaattgtgaa
     3601 tgctctgacc agtggagccg gaaacgtcct cgtttccggg ggcccgtatc gtcgatttcc
     3661 aaatcggcgc catgactgct tgcagagatg actggagcaa agacagagag caccgaacgc
     3721 aaacggagac gtttgcggtc ccatcagtcc tctccaaaac cgctggtaat gagagcggaa
     3781 acctcttcga tcaggtagct ttcatccact ccgtcggcaa tcagattgag ggtggagcca
     3841 gattccgcgg ccagcatcat cactcccatt atgctcttcc catttaccct cataccgtct
     3901 ttttcgataa agaactcgga gcgaaacttg gtggccgctc tcaccatgag agctgagggg
     3961 cgggcatgca ggcccagctt gttactgacc ttgatcttct tttgtatcat tgccctgggc
     4021 ctgagtctgt tttagcagag tctgcttgcg gatctcttcg tggagccggc ggttgaaatc
     4081 ttccgccgcg tcgtaaccca cgcttttgag gatcatgttc atcgccgcca cttccacgat
     4141 cacggagacg tttttgcccg gcgagacggg aagatagatg atggggagct tgacgcccaa
     4201 atgatcggcg taattgctgg tgaggccaat gcgttcgtaa tccatgtttt cctgccaggg
     4261 catcagctcg atctgcatat cgatggtttt ctgacggcgc actctttcga tgccaaacat
     4321 gcgttccaca ttcacaaagc ccaccccgcg gatttccatg aagtggccaa attcgcgtcc
     4381 gctcttgccc accagttggt catcaatata gcggatggtg atgaggtcgt cccccaccag
     4441 gctgtgaccc ctgtgaatga ggtccagagc gcattcgctc ttgccgatcc cgctttttcc
     4501 ggtgagcatg atcccgaggc caaagacgtc caccagagtg gcgtggatgg tcttttcgag
     4561 ggcaaagacg tctctcagat aacgggtgag ctgatgaatg agctggatgg tggaaaagcg
     4621 gctggagagc agcgcgatat tgagatcgtt ggccagatat tccaccaccg gtggcagagt
     4681 cagacctttg gtgacgatca cgcagggaat atccgtctta aaaaggtcgc gcgcccgctg
     4741 gatcagcagc tcttccttga ggctttgcag atagcgcact tccgcctctc cgatcacctg
     4801 aatgcgatcc gaggcaaaca cttccagcca gccggtcaag gccagtccgg gacggtttac
     4861 atggggtgat gagatctttt tgttgagggt ttcgggctcg gtaaccagag aaagggcaag
     4921 atcctttttc ttgttatcga acaactcttt tacggtgatt tcacgcatat tcgcaagcct
     4981 tggggcgagg ccctccctcc ggcatcagat cctggtcccg gagggagata atcgccggtt
     5041 gatcagggtt caaacaattt ataatgctcg gcgtcacgtt tcacgaggac gttcaagcga
     5101 tcggtctcta cattcttgaa gatgaggaat tcttccttca gttcgtctat tttttccagg
     5161 gcttcctgga cgtccataat ctcaggaaca acccgcttgg ttttgagggt tttacgcgcc
     5221 cggctttcgg tatttacctg aatctccgtg gcgcgggaga agtcgttgaa ctgctctttg
     5281 agagctttct tctggtggtc tgtcacacgg tctttgagct tcttgatctg cgcttccatc
     5341 ttatccaggg cgttgtctat ggataaatac atatccatct cttcagcctg actttgcagg
     5401 ttgaagcggg acgcgtgcaa ggtgagctcg cagatgttgc ggttgttctc caacgccaga
     5461 gtgacgtgca cgtgaatgat gtggtcgaag tatttcttga ccttgaggca agacgtttcg
     5521 acgtagtccc tgatcgcctt ggtaagttcg aagtggcggg cagtaatcgt gatctgcatg
     5581 tgtgatacct cctttgttca gatcttgtga atagacaggt tgcggatatc ctccagggat
     5641 tccttgacgg cttcagaaag ggtgggatgg gcaaacacga tggattcgac ggcctcagcc
     5701 gtggcacctt gcgatatcag gatcgcgccc tgagcgatga gctccgcggc attgggaccc
     5761 aggatatgca tccccaccaa tgctttggta tctgcccggg caatggtttt cacgaatccg
     5821 aagctgtttc ccagggccag ggctttgccg cttgccgaaa aaggaaactt gccaaccagg
     5881 atatcggaga atctttcttt ggcctccgct tcggtataac ccacggaagc cagttccgga
     5941 tcggtgaaag tgcagcgggg cacgttcacg tagtccagcg ctagtaaatg aaaatcttgc
     6001 tggctgacct ggctgtggat atgcgaggcc acgatcagtc cttgcttgct ggccgtgtgg
     6061 gccaaaggca gtttggcggt cacatcgcca atggcataga tgccgggcag ggaagtgcgc
     6121 atccatgcgt cgatgactac cgctcccctt tccatctgag gagctgtctg aggccaggca
     6181 atgttcatca caggggaccg tcccacactc atcaggacct tgtccaccat cagctctgag
     6241 ccgtctgaca gagtcaacag cataccatcc ccggaccaca gcgctgaagt gaggcctgcc
     6301 ccggtggtga tcttgatacc agccttctta agtgccaagg ccagacgctt ggagagttct
     6361 tcgtcttcca gggcgacgat acggggtaaa aactcgatga tgtgcacctt gacaccgaag
     6421 ctattcatga tcgaggcaaa ttcgcagccg atcactccgc ctccgatcac ggcaaggctg
     6481 gatggcagct tgtctagctt gaggatgccc gtggaggaaa gcacgttcac ctcgtcgatc
     6541 gcgatgccgg gcaaagactt gggttctgaa cctgtggcca ggatgatcca ctgggcgctg
     6601 atggattctc cctccgcgag gtccactttc cacagctcgc ctactttgga aatggcagtg
     6661 gccttgtcct ttacaatcgg aatcctgcgt ttgcggaaga ggaactcgat cccgctcacc
     6721 agttgttcca cgatctggtt tttgcgctca aacaccctgg agtagtccag gagtccgtcg
     6781 ctgaccggca gtccgtagct ctcggcttcc ttgagctctc tgaagagttc agcgcttttt
     6841 accagtgcct tggtggggat gcaaccccag ttcagacata cgccgcccac gcgttcggct
     6901 tcgatcacca cacaggatat ctcatactgg ctgagccgga tggctgtctc ataaccgcca
     6961 gggcctccgc caatgattgc tacttggtac tgctgcatat tcttagctcc ttctgagacg
     7021 gctgttcagg attcccagtt caaaacggta tttggcgatg atgcgccttg agatgttcat
     7081 gccgagggct ttgaggtttt ccaccagttc ctgatcgctg aggggatggc gcttgtcttc
     7141 cagctccacc atacggatga tgtggctttt cacgtgttgg cgtgagatgg tctcatagtt
     7201 gtcatccatg ccggccgtgc tgctgaaaaa gtccttgagg gcaaagatgc catagggagt
     7261 ctcggcatat ttgtgtttga ccaccctgga gatagtggat tcgctcactc cgatatcctg
     7321 agcgatcacg gcataggtga gaggctgcag aactcccaga ccgctgtaaa aaaaatccgc
     7381 ctggtgcttg atgatggaca gcgaaacgcg ttccagggtg cgcatcctca taaagatgga
     7441 cttgatcaga aacttggcgg agttgatcct gtctctcaca aactggaggg tctcgcggtc
     7501 aaaagcccca cggctgatca tcttgcggta acggggactg ataatgatat tgggagtgat
     7561 ctgatcgttg atgatcacca catattcacc gtcgatgagc ttccaggtga catccggaaa
     7621 cacaaaactg gtgttggggg ccaggattct gagccccggc ttggggtcca gatgggatat
     7681 ttgctcccgg gcgttgaaga tggtctcttc agagacgcta aaatgcgagg ccagcttttg
     7741 atagcggcgg tggatgaggt tttcaaactg gtcggtgcag aggcccacga tgatgcggtt
     7801 ttgcaactgg tcgtccgtaa gctgggagag gaggcattct ccaatatccc tggagctgat
     7861 ccccctgggg ctgagatgca ggatgatctg atgcagttcg ttggcccggg aagcgggaat
     7921 gcggtagcgt ctggccgtct tttccaggtc aaacctcaag ggcagaaagc catagatgtc
     7981 acagctgtcc accagctcgg agcagaaatc cagctcggca tccgtgaggt ccaggggaaa
     8041 gagctgttcc agaaactttt cccgcccgtc ttcctcaaag cggatcaggg attcggtgtg
     8101 atcggcctca ctatcgccat ggcgggattc ataggagagg tgatattcgt tccactggtc
     8161 caggatttcg gtgagctcgc gggcttcgga gatggttttg aaggattctt cgccctcctc
     8221 tttttcttcg atggataccg agctttcagg ctgggaggct tcgaaaccgc tctcgtctat
     8281 atcttcttcg tctttctctt ctctgagctc caggaggggg ttgttgacca gctccagctt
     8341 gatgtagctt tccagctcaa gaatcggcag ggacagcatc ttgagggatt gcagcatctt
     8401 gggcttgagg gcaagctcct gtttttgacg gagggagaga tgttgatcaa gaccactcat
     8461 gattatttat caaagggatt gctcaggaaa cggtctccca gatagacctg cttggctttt
     8521 tcatcattta tcagctccat tgaagttcca gacacgatga tcttaccttc gtagataata
     8581 taagcccgat tcacgatttt caaagtctcg atcacgttgt ggtccgtgat catgatcccg
     8641 atgtttttat ctctcaattt gccaataata tcctggatgt cggccacggc gatgggatcg
     8701 acccccgcga agggctcgtc cataaaaatg aaggcaggat tggtgaccag cgcgcgggtg
     8761 atttccagct tgcgacgttc accacccgag agagtgtagg ctttttgttt cgcgagcttg
     8821 gtgaggccca gttcctcgag cgcttccgcc agccgcttct tctgctcagc tttgggaagt
     8881 ttgagggtct gcaggatcgc caggatgttg tcctgaacgc tcagtttggc aaaaatggag
     8941 ggagcctggg ccagatagcc aaggcccaat ctggcgcgcc ggtacatggg cagatgggtg
     9001 atgtctctgt catccagcat cactttgccc ttgttgggtt tggcgaggcc aataatcata
     9061 tagaaagtag tggtcttgcc ggcaccgttg ggacccaaaa tgcccaccac ctctccctgg
     9121 gacatatcca tgcagagatc gttgaccacc gtcctccggc catatatctt taccaaatgc
     9181 tgagctctga tcttgtgagt ttccatattg ctaatctatt acaagggggt ccatttgtca
     9241 aggattgttc tggaatttat agctgccctt gacgccgcgg gccatgcgca tcaattccag
     9301 cttgttatca tcgttgaact tagcctccag aaaatatccc ctggccgtgt tgacaaaggg
     9361 gtctcgtttg tccctcgccg cctgagcgta ataatagacc acctcatctt ctgccgtgaa
     9421 ttctttgatc gcctcttccc gaaacccgat cgagataaat cccgccgtga cccaattgtc
     9481 ctgaatccca ccccgctctt cggaaaagct cactctacag ctatcctgca gctccgctct
     9541 ctgcagcttg cgctctttga gatagagata gaattcattg gcgctggctt tcgcgtaatc
     9601 tgagaggaag ctcggacggc ctgtaaaaac cgccttttcc tcgttgatca tatagatcag
     9661 aaaatcgctg tttacaaggt aatcctgaga tttgacctcc gcgttgaagg tggccacgat
     9721 cttgcggtcg gcatcgaaga attctatctt gtctgccttg atgtagaggg tatcggctac
     9781 tccggaacgc agacgggggt tttcgatcat gtaggcatag cctgcttttc tgtcccaata
     9841 ggcgtaaccg cactccgccc aggcgttttc atccttgtcc caggctgaga cgtctttcca
     9901 gacggtcagt ttgtccgcct tgcggttgta gatggcgtat tcgcttttga accagcgcag
     9961 actgccagtc ttgcgggatt cggtgatctg caccttgccg cccagattga ggtcctcggt
    10021 ggaacggtaa taggccacgc tatcggcttt gaggccaagg gaatcgttga ccacactcac
    10081 tctgccggag agcctggcga tcttttgcgt gtcaaagatc atcgcccgat cgctcttgaa
    10141 ctctgtcttc ccataccaga aatgcacctt gccgctgagt tccaacacct gttcgttgtt
    10201 gaggcggttg aggaaaagct tgtccgaatg gatcagcttg aaagcttccg tctttgaagt
    10261 ggtttgagct cccaggggaa gcaggacgac cagcagcaag attgccagaa gagcaggtgg
    10321 actaagctgc ctttgcagac aggcaaacga ggacggtggc gtcccaaccg gcaaatggag
    10381 acctttgcca ctcctctgga tcagcttacc agtcaaaatc agtcgaatca aaatttccat
    10441 ccgctgacac tgtttccaac tcggcaaaat caatctgggg attggtgcgc aaacgctgac
    10501 cgcgcagggt gttgccgtcc ctgatcaccg taacgtcccc tggggcggtg atctcatcga
    10561 ggttgcggtc ccagatcatg ctgtttgagc tcaccatgcc tccaggtgag ctaagtttca
    10621 cgttcccata ggcaaagatc atattacggg cgtcatccac aatggtggaa tcggctttca
    10681 gatgagtggt ccctcccgtt ttgggatcga gggccctgat ctccacaccc accgcgttca
    10741 tcagtctgcg gtcataaaag cggtccactc tgtctgcgaa gatttcataa tccacccggt
    10801 cgccgttgta ttctatgatg ttgaccttat tgctgctttc atcgggcagg ttgcggtcca
    10861 aaggggccgc ggccacagtc aggtcctgct gtttacaact acaaagcagc agggccagca
    10921 gcatcgccgc cagcttaaga aatcttggcc aggtaatcgt tcaccgcctg ttcgtagatg
    10981 gtcttgttgt tgagcaccag ttcgatgcat tctctcacgg cacctcttcc acccgaggcc
    11041 agcatcacgt aatcagccat ggcagcgacg tcgcgggcgg cgtctgcagg gcagacagat
    11101 acagccgcca cgttcatcac cgggatgtca ttccagtcgt cgcccatata cagcaagttg
    11161 cagaaatcca gcttgagttc cttgagcagt tctctggttt tttccagctt gttggccact
    11221 ccctggtaga ggtagtcgat cttgaggtcc tgacagcgtc tttccagagc tgcggaactg
    11281 cggccagtga tgacggccgt cctgacatgc gaaaatcgca acagctgaaa gcccatgcca
    11341 tcgtgggcat caaagttctt aagctcttca ccgttacagc catagatgat cttgccgtcg
    11401 gtgagcacgc catcgcaatc gaagattatc aggttgatcc ggtcccaatt gactctgggt
    11461 ttatagggtt tgccgatttt cttgaagtgg gtaacctgat gagcgaattg atcatactca
    11521 tgcgggagta tcaccttgtt gtttaactct gccatttagt cctccttcaa cgactttctc
    11581 acggcaatcg cgtctgccag caaagccttc agatcatcca gaggtaacat gctggccgcg
    11641 tcgctgagtg ctgtgtgggg ctggggatga gtctcgataa acagtcccct cactttacca
    11701 gtggccatgg cggccctggc catcatggcg gcatactgag gcccccctcc ggaaacctgc
    11761 cccaatgaag gtttctgcaa ggaatgggtg acgtcataga cgatcggata gccggattcg
    11821 gccatgatgg caaagcccct aaaatctacc accagatcat gatagccaaa gctcgtgccc
    11881 cgctcggtga gcaggatttg gtctgatccg gcatttctgg ccttaatggc ggccgggatg
    11941 atatcctcgg gggccatgaa ctggcctttt ttgatgttga taatcctgcc gctctttgcc
    12001 acggcctcga tcaaagaggt ctgacggctc agaaacgcgg ggatttgaat gatatcacag
    12061 atctgggcca ccgctttcac ttcctcacat tcatgcacat cggtgagaat gggaaagccg
    12121 aattcccgct tcagttcatc cagacagcgc agccccgctt ccattccagg cccgctggga
    12181 gagctgcctg agctgcgatt ggccttgcgg taggagctct tgaacacgag cgggagctta
    12241 aactccgcgc tcaggaagct gagccgttcc gctacctggc gcattgtgtc catcccctca
    12301 accacgcagg gacccgcgat cagaaagaag ggccaggcct cctttaatct atgatagagt
    12361 tccatttacc tcgcctaaga gagctttttt atctgttccc agctaaaact ggcatcgtca
    12421 cgaccaaaat ggccatagga agcagtggga agatagatcg ctctgcgcag atcaaggtaa
    12481 tcgatgattc catttactgt caagtcaaaa ttgccacgga tgattttctc gatctcatga
    12541 tctgccaaaa ctccgctgcc ataggtatcc accatcaatg aaaccggctc ctgttcccct
    12601 atcacaaagg aaaactggat cagacattca tccgccaatc cgcaagccac cacgctcttg
    12661 gcaatgtgcc tggccatata ggcagcgctg cggtctacct tggtggcgtc cttgccggaa
    12721 aatgccccgc caccgtgttg agcccagccg ccataggtgt ccacgatgat cttgcgtccc
    12781 gtgagtcccg tgtcggcagc cggaccgcca tcttcccatc tgcccagggg attgatcttc
    12841 acggcgtatt tatcggcgct gaaagtgctg aagctgtcag cttccatttc agcgatggtg
    12901 ggcttgatca ccagttcttc tatcaactgc cgcaaatcct ggaagccctt gtcatccaaa
    12961 ggatggtggt gggtggaaat caccaccgtc tccaaagcta taggtttgcg gccacagtaa
    13021 cgcatgctca cctgagattt ggaatcaggc attaattgtg ggatagcgcc gtttcttcgc
    13081 gcttcgctga gcttttgtaa gagcttgtgg gccatggcga tgggaacagg catcaggtgc
    13141 cgggtctgat tgcaggcata gccaaacatc agtccctgat cgccggctcc atttgattgc
    13201 tccagtgctt tctcctgttt gtggaggtaa ttctcgatat agcagttgtc gtcaaaacct
    13261 ttcccgtcaa ccgtataacc gatgtcggca atcactttgc ggaccagagg ctcgatctcg
    13321 accttaacct gggaaggcga ggatatttct ccggccagca ctacccggtt tttggtgcat
    13381 agagtctcgc aggcaactct ggccttggga tctttgccaa caaaagcgtc cagaaccgcg
    13441 tccgagatct ggtcgcaaac cttgtccgga tgaccttcac tcaccgattc ggaggtgaac
    13501 acatactcgt ttttgtcgca ggggagattg gcatgtaaag ctgtcatctc tattctcctt
    13561 tttttcaatt acttagtatt aacccaaagc aaaatgaccg gccactaaag ccggtcattc
    13621 atagagaatc agccgcactt tagcacattt gtcacgcgga gcaaagggca aattaccgcg
    13681 atatatacac tgccttgggc actggcaaag atcctcgcgg ataccaggct tgaagacaga
    13741 aaagggtggt acatctccgg ggattcgaac cccggaccca ctgattaaga gtcagttgct
    13801 ctaccaactg agctagagat gcacaacttg agggacatct ttttttaggt gcctgggatg
    13861 tcaagataaa agacggggaa gcgttacgag taggagctaa gcgagtggcg ggtgaatctc
    13921 ttttttcaat ctgtgtactc accgctttct gtgtcctctg tgaagtcagt tcagatgatt
    13981 agaaaaagcc ggtggaagca tccaccggct ttttcagtct atctcttggc tctttatctc
    14041 tttgttagga attcatgtta caggagctca atgttttcgg ctgtgatgcc cttgaaagca
    14101 ttgcgggtat ccagaatggc cactccgctg gccgctattt tggcataatc gagattggag
    14161 tgatcggtga tcaggatgat caggtcatat ttcttgaggt ttccgaggga cacggtctga
    14221 tacatccggc cgtttttctc atggtgaaat tccggcacat agggatcgtg gatctcaaaa
    14281 tcggccgaga ccttttcgag ctgagtgatc acaccttcga tgggggattc acgcatgtcg
    14341 cggatgtctt ttttgtaggc agcgcccaag agcaggatct tggccctgga gatagccacg
    14401 ccctgtttat tgaggatgcg gatggcccgc tggaccacaa actccggcat ggcgatgttg
    14461 atctctccag ccaattcgat gaggcgggta tgaaaatcaa actcgcgggc cttccaggtg
    14521 aggtagaaag gatcgatggg aatgcagtgt ccacctactc cgggaccggg atagaaggcc
    14581 ataaagccat agggcttggt cttggcggca tccaccactt cccagatact gatgcccatg
    14641 cggtcgcaga gcacggtcat ctcgttggcc agggcgatgt tgatgttgcg gaaggtgttt
    14701 tcatagatct tttccatctc agccacttcg ggagatgaga ccagatggac gggtgcgtcc
    14761 aacacactct cgtaaaagag cttggcgatt tcgttgcagg cgggggtcat gccgcccacg
    14821 accttggggg tgttgtgggt tttgtaggtc tcgttgcctg gatccacgcg ttccggtgag
    14881 aaggccacat atacatcctc ccccactttc agccccactt tttccagctc cggcacgatg
    14941 atctcacgcg tggtgccagg ataagtggtg ctttccagca cgatgagggt atgtttgttc
    15001 agatgcggtg ccatcgcttt ggtgcaggct tcaatgtagg tggtatcagg ctggtggtaa
    15061 aggtccaggg gagtgggaac cgcgatgagc acgatgttga cctctttcat acgggtgtaa
    15121 tcatctgtgg cggagagctt ttgggagctc acaacctcca ccagatgagc gtcttccacg
    15181 tctccgatat agtttttccc ggcattgaca tgctcgatgg catactggct cacttcaaat
    15241 cccaacactt catagccttt tctggcaacg gtcacggcca tgggcaggcc cacatagccc
    15301 aaaccgacca ctccgatctt gacactcttg ttcttaacgc tttctaataa tgacattata
    15361 cctccacggg atgaatagaa taagcgactg gctgctcctt gaaagacaac ccttcaggct
    15421 tgagacacaa attttctgag gccggtttct tgtaaagatt attccatcag ttgctcctgg
    15481 aagcgggacc ggtttcccat agtctgccca gtgggctggt cccgaatagc tgactgctta
    15541 gccactattg catgatataa tcatggtttt ggcgtaaaga ttgcacgtgg aatttatatg
    15601 tagtgattac agtttttttg taatgaggat aggatatgaa acttgatgaa ttgttgctgc
    15661 aggccgatca agatcacaat tccggcctga actgcgccca atctgtactg aaacagtttt
    15721 gtcagtccgg agggttgcca gcggagaagt attttttacg tttgagctct ggtctgggca
    15781 gcggcatgaa agtgggtagc gtatgcggtg ctctgagcgg agggatcatg gccctgggac
    15841 tgcatctggg cagcgaggat cccgcccgca aagccgaaat ggatgcccct gtccgtgaac
    15901 tggttacgag gttcaggacc acaatggccc ataaggagtg caacaaaatc atcggctatg
    15961 acgtgtccga tcccactcaa cgcgccattg caagggagaa gggcattttc gcaagccagt
    16021 gtcccctggc gatccgcact gctgtaacaa tcgtttacga tatcctgaac cgtaatcaat
    16081 agcagccatc cagacaagcg ttttccccat ctgaccagcc aggaggcggg cttctgagtc
    16141 tcagattttt gccagtttca gcttaccgca tcagcattga tactcttaaa gcaatgagcc
    16201 acaatttatt aagccgttct taagccattt tatccatacc ctctttctcc ttccttcccg
    16261 catcctaata cggactcatt acggacttag tccgtaatga gtccgtaatg ataccgtaat
    16321 gctactcggc aagggagcag ggccggatat ggggctatgc tgactaaagc gtatatttaa
    16381 caggcataca catctaaatc gccctgactt tcaaggtcat aacagcgatt ggaataagga
    16441 gtgatatagg atagctatct gagcaaaagc accctgcggt tgatcacctg atccccggtt
    16501 tgggccctga tcagataaag gccggagctt aggttcgcag cgtccagggt gaagctatgg
    16561 accccctcgg acaggtaagc gcgctggagt tccaggattt tctggccttt caggttatat
    16621 agttccaccc gtgtatccgc ggccctgcca agctccagac tgacaaggga actggtcctg
    16681 aagggattgg gtgcaacgtt aatcctggag aggcacagct caggtgccac gggatcgtcg
    16741 gtatcgactg gtcccacaaa cacttcagtg aaaaccggct cggatacgcc catggaataa
    16801 gtagccgtgg cagagaaatt gtagatccct tccaaagctg gttcggagta tcccagcaca
    16861 ctcgcaggca attgaacgat ctcagttccc tccaggtaga gcgtgacggt gtggatgtca
    16921 taaggcgttt ccgggagaga ccaatctaag gttaccgccc caatggcatc atagccatag
    16981 aaatccacca ggggttcaca gagcgtctgg gtatgcaccg tccagccgag gtctttgaga
    17041 atgcccagaa cgattggaga tggatatctg gcccctccac ccacgattcc ggttggcact
    17101 ccggattcgt tttcggtgct gttatacgtg gtgccggata gcctggcagt tctggccaga
    17161 ttgaaagctc cggcatagag aacaggttca gtgcctcctc cccaggcttc agcccattga
    17221 ccttcataac gcagatttcc acccgtcaat tgggcattta gagttgtgga gggattggtg
    17281 aagatggcgg agtcggtaag gtgctgaccc tgggtgttga cgatatgggt gtcgtagagg
    17341 gcaggctgtc cctgcatggc ttcccaggga aaagaggtcg tgagccccag caccgatggg
    17401 tccagcattc cataggagcc atagccctgc tgcacataaa aggagggcat gtatcccagg
    17461 ccataggcca cggctttgaa gacctcagtc acaaaatcat agctgccggc ggggcagttg
    17521 tcgtccagtc cataatacca gggatgatcg gcagtgccca caatgaacat gtccatatcc
    17581 gcctctccgg gattgagttc ggtgccggca atcatgttgg ccatggcagt gcaataccag
    17641 atattgctct gcggggcgga agtgaagttg cggaccatgt ttggaacgaa caccattaca
    17701 aagccgggga tttgctgaaa tctggcattg atcttgatgg ggacatccga atccagaagc
    17761 ggttcccaaa gcgagacggc atgttcaaaa gcggccatct gcagagcgtc gaaacccgtg
    17821 tagctcacct ggaagctcac gttggccatc acggcagcgc agtaaagaca aaaaaccaaa
    17881 gcaatcaggt acttcatgac attcctccag aatgttttga gccagaaaac ccgtaacagc
    17941 ttagcgtgtc aacaaaaatt gccgcctcag cagctaaaaa cacatatttg ggtggaaagc
    18001 tgtcaacaag ggataaattg tgcttagcag cccggccgga gaaatagcgc tattgagacc
    18061 gattggctat gataaactgc tcgatcagat agccaaaggc cttggaaaac ctgccttccc
    18121 tggccagaga aaaccaggcc ccgatcgcca cgatcaacgc tcccacagca tccaaaccca
    18181 gatcctgcat cgtgttgatc agggcccggc cataatcgta aacgcttccc gggagggtgg
    18241 aatccctgcc ttttagcatg ttgaccccga gaagctgatc agctccgaat tccagcagtt
    18301 cccataaagc tccgaaaccc atggtgaaaa agaagacgca tacggctggc agaatgggtc
    18361 tcacgccact tgacttcata tctttgccat aaaggagcct gaaacccaga taagccacat
    18421 aggtgatcat cattgccgaa caggtatgca aaagatcgtc ccaccacagg aaccgataga
    18481 aaaaggaatg gatctctccc agatacatgg cggcgatgat gaagcccaga tagaccaatc
    18541 gccaggatac cggcagcctg gtcctgagca gccattgcag aggtttggcc aactgagcaa
    18601 ggctagcgca caatatggct gatgccaggc taacattatc attggcaagc atatacctat
    18661 aggcggccca gaggctgagc agcgtgatta tccagcggac ggtggtctct atgacgggca
    18721 gcgtgattct ggatttgggc atgataactc cagtttagat tcggcgcgga aacgagcaag
    18781 ggtacaggtt cccgcagatt tcgcagtttt tcctgcggat ttcgcaagat tatccagaga
    18841 gtggtgggga agtgagcacc gttgtttctc aaccttctca accttctcat ctgtatcaat
    18901 atccgcgtga tctgcgccaa aacctgcgaa atctgcggga aacatgcccg ccttctcaac
    18961 tttgtcaata aacgacggcc tccaatcctg gaggccgtct gtatcgattg atgatggttt
    19021 attggctgag caccatcttg cgcgtctgct gatacttagg cgcgctgaga cggtagtaat
    19081 agactccgct ggaacaaggt ctgccgctgg agtccttgcc gttccagttc acctgatagt
    19141 gaccggcggc ctggtccacg tttaccagtt gttttaccag ctgtcctttc aggttgtaga
    19201 tttccagttt gaccgctccg gcatctttga tgctgtagct gatcacggtt tccggattga
    19261 aaggattggg gtagttgcca ttgagggatg tgacagtcac tggcgtgagg tcatcggcat
    19321 tgggagaacc ttccaccata atgaagttta cagtggtagt ctggttgaga ttgactacca
    19381 catcttcgat ggtttgggtc acaaagccgg tcgcggaggc ggtgacgtca taggtaccca
    19441 cgggaacgat gagggtgtaa gctcccgctg agttggttgt ggcactcacg cctcccgcag
    19501 tgacggtggc ccccaggatg ggagtggtat tctgacgtct caccagacca gagatcaggc
    19561 cggtctgcac gatcttttcc agcatgttcg agaaggaagg caccgaggcc acgtcagcgg
    19621 tatacagagc tttgacggcc cagcgatagt ttccgttggg cagcagctgc catcccggat
    19681 cggaatggtg aagcagggtg acctgttcag gtgtaagcag ggtccagctg gcttcgttta
    19741 cttcctggcc cgcgctgagc ctccatattt tgtatccggt cagagatctg tccgatgcgg
    19801 cgtgagtctt gagctggtat ggcatgggca ggctgggctc ggtccgctgg cttccttcct
    19861 gcatgcgacg ggaaggatga gtggtggcca cggggttgaa gttaaagccc tgtccacccg
    19921 cggaacgggt ctcaaactga tccatatcga agcggacgat tccatcagga tgtcccaggg
    19981 gcagaagggc atttccgata tagaggtcat caatgaacca cacataccac aatccgtcgt
    20041 cgttgggacc atcagtggca ttgaaggcca gcttgatctg cattcctgaa taggccgcca
    20101 ggtccacggt gatgggagag gcataatagt tccagccacc cgtctgagtc gaggcatccc
    20161 acaggactgt ccaggtgttt ccgttgtccg gtgaaacttt cacataatag tgatcgttgt
    20221 tggtggatcc caggaacaca tagctgtcga agtacagata tccagagggc gggcagttga
    20281 aggcaggagt gatcaaccat tcatcctggt gagcataatc ccaccagaga ccggcctgat
    20341 agctgccttc ggttgggttg acgggagatc cgctaatggt gagcgcgccg gccctgcacc
    20401 aggtgggata gactccgctg gtattgatgg ggccggtgtt ggtgatcgtc tgagtccagc
    20461 cctggggcgg gaaggtttcg gattcgaagc tttccgtgac ttcaatggca ttgggatcag
    20521 gactctgcca gctgagatcg acggatgtgc cgttcacagc ctcttcagcc tccacagtgt
    20581 gaggcgcgta ggccacttcg ttaagagtga cattgcccat gctgtagttg gtgccggtga
    20641 cgatgatatt gccattgaca ctggtgtagc cgggacacat gatcacatag gcataaggtt
    20701 gattggcata gacgctgggg atagtgaaag ctcccgcggc gttggtgttg gcatcataat
    20761 tggcataacc ggccagatgg atggacgcgc cagccaaacc agctcccgtg tcactggcaa
    20821 tcacagtccc gctcacgttc acggtagcca tgggctgcat ggtgacattc aggatctcgg
    20881 tctcatcttc ttcgatggta accgtttggc tctgattgat atggccatag gcgctgaagt
    20941 tgatggtata ggtttctggc aggacgtttt ggatgctgta ctggccctgg gcattggtag
    21001 tagtgccata acctcctgtc tggaattgaa ctatggcacc ttcgatgggc aggttaccgg
    21061 ctcccagcac agtaccgttg atgtgtccca ccccacctgg aataatgtag aaggcggttt
    21121 gggcaaactg gccagtgagg gtacctgcgg ccgtcatatt catgggatcg taggtggtgg
    21181 agtcgctgga gatcttacgg caacggttgc tgccaatggt ctgggctttg aagttatcag
    21241 cggtgttgta atactgagtg tccatgggac ggtggaaggt gagcaccagg ttttggccat
    21301 ccagatacat atagggagtc gcgaagggga tcgtgatcac attcgcgccg gatgggaagt
    21361 tcagcgtgcc gttgaacacc tgggtgtgac cgttgatggg aatccatccc gcggacaggt
    21421 ctgtgagcgg agtggtgcca atccatacac tgatcggcag attctgcaga ttgctcacga
    21481 actggttgtt caaacccagc ccggtgatct gacccatgac gtttccgatt tcagcgggga
    21541 agatcaggta ttgatgcacc gagtttctgt agtacatgtc tatgggggtg cgggcattct
    21601 ggcttccgtc cccaaccgtg aataccatga ttccggcagg ctggataagg atgttgaaag
    21661 taccagtttg gtcgttgagg gggttctggt ctccggtaag cactactttg ccatatacag
    21721 atgaagctcc ttccacggtg ggggtccagg tgaccggtac atccactgcc atgccaggat
    21781 tgacctgaac gccaggcgcg gacgcgagtt ccacatcccc aggcccgaag agcttgacct
    21841 gatagtcatt ctgagggttg gtgccccagt tgaagatccc caccgtaaag gtggcaggca
    21901 tacctaccga gggagtgctg ttgcccatga tggttgtggc agccagatcg ttgtccggga
    21961 tcacttctat catgacatca tccagataga aggtgcgtga agtgcctccc aagccgtgtt
    22021 tgaaggcgat cactctgccc gtgccggtgt aggcggcaaa ggtgaccaca aactcctggt
    22081 aggtgctggt cagtgtgaga gtctgcactt cctggaaggt gctggcatcc tgatagtctg
    22141 tcatgacgcc aacgatcaag ggatatccgg tggcagaacc tgaacggccc cagaacttta
    22201 ctctggtagt attggtattg atcgtgttgg ccaaaggcgg cgcttccaaa atcaccgtcg
    22261 cgttggcatc ggtggagttg agcaacctga cgcaattggg ctgagagtga gcgtaggtgg
    22321 tggaggcgta ggtgtttaca tccgctcccg tcaccgtggc ctgaaccagt ttggtccagt
    22381 ctacaggcag gtccggaggc gttacgccat caaagttctg gctgtatggc agggcagtga
    22441 tcgtggtatt gatgcagttg gcggaaagag gaatggtatg agtctgacgc gccatgttgt
    22501 ccacgatggt gatggtgccg ttgtgaactc ctaccgcggt ggggctgtat tgcagagtga
    22561 agtctagcgg ctgtccgaag ttcaccgtcg cggggaaagc aggaagattc tgcaaggtgt
    22621 aggcatccgc tccgcccacg ctgatactgc tgatcaccaa aggtgctccc ccagcgttac
    22681 ttacagatat ctggcggctt tggttggaat taatcagcac cgtgccaaag tttaccgatg
    22741 agggattgac ggcaaagact ggcgtggtgc ctgaagccac catgtggaag gtggtcatgg
    22801 ggaactgacc tgagagcgtt ccagccgctg agggagcggc aggatcatag gtagtggtat
    22861 cggaggtcag ttttctggca cgattgctgc cgatggtctg cacccggaag ttgtcgttgg
    22921 tgttgtaata cacatcttcc cagggacgat tggcatagag caccagattc ccgctcatgt
    22981 agttgaaggg ggtctgaagc gggatggtta cggtgttttc gccgctggga taagtgatgt
    23041 tgccgtcgta taccaaagtc aaattgggag gcaggatcca gccgcctgag aggtcggcca
    23101 gatcggtctg tcccatccag atcttggtgg gcttgtctgg caggttggtt acgaagttgt
    23161 tatagaagct gagggcggtt atactgccgt atgatccaag ctcagcgggg tagtacatac
    23221 actgataaag actgtttttg taatagaaat ccaccggacg gccttcagta gctgtgccct
    23281 caccaaccgt gacggtatag actcccgccg gcatgacgga aatattgagc ggggtggtct
    23341 catcgttggc cgggacagcg tcgtttggca gcattacctt accggtaatt gccaaaggac
    23401 ctggagcgga tggattccag gggattacaa agctgtgagt ttgacccggt tcaagcgtca
    23461 cacctgccac ggaagcaagt tcggtggcgc cggccatcag ttttacggtg taggccgtac
    23521 ccaactgagt ggtcgtgccg gtattctcca cttccacagt atagttggag accattccca
    23581 ccgatggagt cgatgtgccg ctgatgctga gggccgcgag gtccagcctc agaatgcgga
    23641 tcaggtttga gaaggctgga acgctgagca cgttgttggt gtaaacaccc ttgatggccc
    23701 acttgtaatt gccgtcaggc aagctggccc aggcactgtc cacatagaac gtgtcctgga
    23761 tggcgttggt ggtcaacaag gtccagctgg tctcgttggt ttcgttaccc tgggtgagac
    23821 gccacacttt gtaaccggtg agcacccgtt gcggctctct ggtcctggaa tctgactggt
    23881 attgggaagc ggttctctga gagcgcgctt gtttgctggc cactccatcg ctttccagac
    23941 caccagattt gaatccctga gagggagtat aggtataatt gaccgtccgg cccatgggag
    24001 cacccacata caggtcgtct atataccagc ccgcatagga aacggtaccg gtggcatacc
    24061 actcgaagct gatctcagcg ttggcctggt tggcataagc tgacagatca atggtaagac
    24121 gctgccaggg cataaattcc gcggccgcgc tggaacccca aacggtggtt ccgttcactt
    24181 tgatcaaacc atagtcccag gtgttgtagc catccatgta gtgccagaaa tccagcacgg
    24241 gattggcata gccgctcagg ttgaacgtct ttttgaggta agaccaaccg gtggcgttgg
    24301 tgtaaccgcc ctgcaggacg gttccccaca tgccagtacc ggaatgagca gcggcgggtg
    24361 gagcatccac ataggtatct atgtctgtgt aattggctgc gttgtattca tttccccact
    24421 gccagtctcc caggggattg gtccagtttg aggtgggtac ccagcctccg ttgtctactt
    24481 caaaatcctc taccccaccg ccaccggttc cgccaggtac acgccaggta agcgcaacct
    24541 gagtttgagc ggtattttcc gtggctacta cttgccgggg tggaagagcg atctcgttca
    24601 tggtcaggga gcccatgtcg tagctggtag cccccaccgt gatggtgcca ctcaggtctt
    24661 gatagctctc tttgaccagc agatagttat agctgttgcc ggaaaggacg ccggcgatgc
    24721 tgaactgacc agcggcattg gtggtcccgg aataattcaa tggaccactg aggcttactt
    24781 ccacgtcagc cagtcccaca gtgggctggt cactgccata caccgttccc gagacggtca
    24841 ctgtggatga agagaccaga ttgaagttca ggatggcagt ctggtccgcg attagggtca
    24901 caggcagagt ctgggtctca tagcccagct tgctggccgt cacagtatag ttgccaggct
    24961 gcaggaaggg gaagctgtaa gcaccgctgg cattggtagc agtctcgtaa gtagtgtcgt
    25021 tgaccactat atccacatcc gctacggggt tgccacctga agtcacagta ccctccagct
    25081 ccgcaaaacc tgccgtgaca aaggtgaaag aggttttcgc gaaggtgccg gaaagagttc
    25141 ccgcggcgct gggggccatg ggatcgtagg tcgtggaatc cgaggtgagc ttgcgggccc
    25201 ggttggtacc aagcgtctgg gcctggaagt tatcactgct gctgtagtag actgtgtccc
    25261 aaggccggtt ggcatagagc agcagattgc ccgctgtgta tgtgaaggga gtttgcagcg
    25321 gaatggtgat cgtgttttgc ccggtgggga aattgatcgt gccgtcatac accagggtca
    25381 gaccgtctaa aacccagcca ccgctcagat ctgtaagatc cgtctgtttc agccagaact
    25441 tacagggcat gttctgcaga tcggtaacga agttgttgta gaaggtgagg gcggtcacgt
    25501 taccatacac gttgagctcg ttttggaaat acagggcctg gtgcaggctg ttcctataat
    25561 agaactccaa cggcacgcct tctgccagat ttccttctcc cacagttacc aggacggccc
    25621 cagcgggctg tacgctgatg ttgtagttgg ggctttggtc attggcggga ttggcatccc
    25681 cgggcaggat tactttgccg tagagggcca tggggccctg ctcggtgggt gtccatgtga
    25741 cagggaccat cacggtggcg tctggatcgg cactcacccc tgcagcgctg gcaagttcta
    25801 cgttgtcagc gttgtagagc ttgacggtgt agttatcctg aggcaaagtc ccccagttct
    25861 tcaccgccag cgagtaggtc gtggcgtttc caaccgaagg agtcgcgttt ccagtcacgg
    25921 ccaggcaggc caggtcgttc tgagcgatct gttcgtaggt gacatcatcc acatagaggc
    25981 tgcgtcccgt ggctcccaga ccatgcttga aagcgatctt ggtgcccgtg ccggcatagc
    26041 ctgtgagatt caccgcgtat tcagtaatag tggttgtaag agcgatgctg gctacttccg
    26101 tataggaggt gggatcctga ggatcgttga tcacgccaat cgagagagga taacctgccc
    26161 cgctggcacg agcccagaat ctcactctga tgctgttgac cgtgagggga gccgcgatgt
    26221 ttggccccac cgcgatcagg atggcatccg cgtcgctggg attatacagc ctgaggcagt
    26281 ttggctggct gtgagcgtaa gtggtggaag cgtatgaggc tacaactgca gaagtggaag
    26341 tggattggat gatcgcgctc cagtcaaatg ggagggccgg ggccgtaacc gcatcccaat
    26401 gctgcagata tggcagggtg gtcacattgc tgtcgccgtg ggtggtgaaa ctccatacgg
    26461 ggcagttggc tgcgtcgcca aactggttgt agggtactat cttccagtag taggtggtgc
    26521 cgatctgaag gtctggatcg ggatcatagc tggtcacgtt ttgaatgtca acgttgttga
    26581 cgatgttggt gggaggattg ttggtcccca aagaaagcct gtaaccgttt gggaaaccgt
    26641 tgacaggaac ccagttaagg acgacagcgg gagatacaag ggtagctccg tccgtaggat
    26701 aaaccgcctg agcgggatca ggaggagtgg tctgcggagt aacgatttga atattgggac
    26761 ggctgtaggt gcgagtcccc gtgacggcgt tggggaaaga attgtcctgg cccttataga
    26821 ccgtcaggta gtttggcgta gtgcttgtat agcggaaagt cgggtagccg gtcacgtaat
    26881 ccgtgtccca gttttcaaac agaaattcga tgttctgaat gccgtcccag ctgaatggag
    26941 tggtgaacat gatataatgc cagccccctc cattatatgt aagattacct tgaaaaacct
    27001 gttgatatcc agtgacgccg ggataggtgt tatcggttgt ctcatagaga cttgcggtag
    27061 tatgacgggc ataaacgcgc tgatccagca tttgatattg gctgggggta ttgcccacgt
    27121 aaaaaccaac cccagtgata tttgccgccg cggttaagcc cgcgccactg atctcggcct
    27181 gggtgtagat gatcttgctc cagctgtaat cgtacaaccc gtagtaaggg cttgtactgg
    27241 aagcgctagt gcctgtaccg attgtataaa catcggccat ggcgaagctt atcatcagca
    27301 agagcagact gagcacaaag attcttttca tctcgtctcc tttattgtgt tttgtagctt
    27361 taaaattctg gactgacaag cgcagtccgg agggggggca accaagctgg tggcgatcca
    27421 taaaggattg ccggccagca ggataaaaca aatcacgcta ttcacctgaa tgggtaaaaa
    27481 cccaatagtc ccaatcgtat ctcggcccct ctatttgtaa agcaaaaaaa caaaaatcat
    27541 gtagcttggc tgttgccaga tttacactcc ctcacagtca attagattta tggtaaaata
    27601 tcgctgtaga aaatgcttgt ttgcagaatc tgcggctgtt aagcaggatt ggatgagcag
    27661 caatcttttt ctgcttgaca caatctcagc attcccgctt catgcttcaa gtgatttacg
    27721 cccttggttc gaattgataa gcagacctcg gggctacaaa gagattggga gtgtttatgc
    27781 cggctaatat ttacgtgacc cgccgtattc ctcaggcggc gatcgatctg ctgagctcat
    27841 attttgatgt gagtgtcaac ccctttgagc gaaatctgcg cagggcggag ttgctggagc
    27901 atatcaaaga tgcagacgcc atcctctgtc tcctgtcaga cagcatcgac cgcagcgtga
    27961 tggatgccgc gccccgcttg aaatgcatct ccaactacgc cgtcggctac aacaacatcg
    28021 atgtggaata tgcctccaaa caaggcatcg tggtctgcaa cactcccaaa gttctcaccg
    28081 agacaaccgc cgatctcacc tgggcgctga tcatgagctg cgccaggagg attgtggaaa
    28141 gcgacagctt ccttaggtcc ggtgagttca aaggatggga acccatgctg ctcatgggag
    28201 cggatatcca tcacaaaacc ctcggaatca tcggaatggg aagaatcgga caggctgtag
    28261 ccaaacgcgc ctcgggtttt agcatgaaag tgctggctta tgatccatcc ctgccagagg
    28321 accacaacac cgctttgtgg aagtccacga accttcagac gctgttgcgg gaagccgatt
    28381 tcgtgagcct gcacgtgccc ctcacagaga aaacccagca tatgatcggc cccggtgaac
    28441 tgaaactgat gaagcgcggc gccatcctga tcaacacctc gagaggggcc gtgatcaacg
    28501 aaagcgctct gatcgaagct ctcagacaaa accggatcgc ggccgcgggc ctggatgtgt
    28561 atgaaaaaga gccttatatc ccgcaggaac tgctggaact gaaaaacgtg gtgctattgc
    28621 cccacatcgg ctccgccacc acggagacca ggaccgccat gggcatcatg gctgctcaaa
    28681 acgccattgc cgtcctcaaa ggcgaagaac cgccctccag gataaactaa gctttcactc
    28741 acgctatcaa gaggttatta tgtccggcat catctttttg cgcactcgcg ttctcgatca
    28801 gcttaccacc ttttacacag agacactcca gatggagctc tggctggacc agggtgcctg
    28861 ccaaatcttc cagaaaggca atctgctgct gggattctgc cagagagagg aagccgattt
    28921 ggggggcacc atcaccattt tctttcccga ccgggaaagc gtggaccagg cttatcagcg
    28981 tctgggcaaa atcgcgctgg ccgctccagg gctgaatcca aactatcata tctaccattt
    29041 ctacgcccgt gatccggaag gccgcagcct ggaattccag cattttgagc atgccctgaa
    29101 tccctatcat actacggtgg aaagccttat gacccgtagg agcatccggt cctactcatc
    29161 agaaccggtt cccgaagcgc tgctgaaccg agtctttgag ctctgccgct actcccccac
    29221 cgccagaaat ctgcaggcct actactatat tgtgatcaaa gatcccgcca tcgtggatag
    29281 gattgtggag ttgcgtggcc cggccggaga acctttgaga gcggcacccc tcacgatcgc
    29341 ggtggccgcg aagggagaaa tctcacgccg ggtggtgcag gatgcctgta tagctgccta
    29401 tcatctgctt ctggccgccc acacttatgg actgggcacc tgctgggtca ccgatatgga
    29461 caacggcccc gtcaaagacc tgctgggcat tccccacgag gattatgtgg cctgcctcac
    29521 acctttgggc tggcctgcgg agcacaaacc cgtgccccaa cgccatgagg tttcagagtt
    29581 tgtcaggtat ctgagctaag ctttcaaacc tcactttcat tcacttttca ctattcacct
    29641 ttcactcctt aagcctcctt tgcctctcct ttacatcact catgcctatc ccgtgcttca
    29701 gtaaaggtga ggcacaggac aggcactgga tagctgaaac cgggtatcgt cagagcaggc
    29761 ccggcccaaa agccatttta gcggcttggg tccgggatgc tggaggtcac gaggaggaca
    29821 gtgagcaggc aagcgagcgg aaagctgagc tcagccgctt tacctgatca ccagaaaccg
    29881 cctggtgttt gaggctcccc gctggtcgtg aactctgaga aagtagattc cgctggctgg
    29941 cacatccacg tccagcaaca gggaggcttc ccttccggaa gcgttgtagc tgaccacttt
    30001 ctggcccctc aggttgaaaa tctccacttt gtgcaatcca tttgccgcct cgatattcag
    30061 cttttggccc ctggaaaccg gatttggcca cagcttcagg ctcagggaaa ctggtccctg
    30121 aagctcatcg gagctggaaa cgttgaaatt gaaaagctct tccctgtcca gtgacaaagc
    30181 ggaattggac caggcgggat ctgtcagagt ggcagcagtc cacagaatcg ctcccgtcga
    30241 aggagtgtat tggagggcgg agaggttgtg agtctggccc gccgcgccgc tcaggacacg
    30301 ccattgacgc ttggcgctga tctgatgatt ggcgtagagc gaatcctgga tagacgcgta
    30361 acgctcgcaa cagatggtcg agccaccata gcgaaagtga ttggtggcgg ccagatggtt
    30421 accgggcagg ttgccgtatt ggttgaatct cctcagcacc gtgttggtgt tgctggtctc
    30481 gatcaccatg gcgttggggt ttccctccag ctcgtccaaa gcgtggatga tggtgccgtt
    30541 gtaatgatgg ccctgcgcca ggcagtgata cacatcctcg gctttattga cgccatcatc
    30601 atccgcgtcg atgagttcca gtccgtcccg gatgtcgaac agcacgtggg tgaggttgtt
    30661 gggagagggg ttgttgtagt cgttgcccat gttcaagaaa gccgcggtcc cgctctcatt
    30721 gatggcagag agggctccca gcaagccagg aaaggtgaag ctcatccact tctgttcgtc
    30781 gttttcgctg ggatggtgga ccaccaggag cggattggcg atcagagcgt cgttggagct
    30841 ccagtccagg aatctggtga tcacagccga gccggccaaa agactatcct gctgggtgga
    30901 tatcccccag ctggaaaggg aggcgcagcc aagctcaagc ggagattccg gtctgagctg
    30961 gttcctgatc tggatcatat ccaccaccgc gtttacctgc ataacgtcct cgggagtgag
    31021 gtttcgctgc aggccatcgt gccagagact ggaaccgctg gcagcgatgc cgtcgatcag
    31081 gccctccgcc tccagataga gacggggatg aaagctgaaa tgctgctgat accagttcca
    31141 gaaatagtca tacgcggtgg gtgatccaaa ggcaaacatc gtgtagtagt aaccgtggaa
    31201 cacattcata atccgttggt tgagcaggta gccctgcgcg tagccacgct ggtagtggct
    31261 gccccacact ttgagcacca gtttgtcgcc atactggctc aattcgcctt gcggctgagc
    31321 ggaaatcaga ccgatccaca aaaccaggac cgcgatgaac agagctcgga atatcttcat
    31381 attattatac ctattggatg attgatgtgc agacagagta tgcaaaaacg aggccatggc
    31441 aaggctaaag ttgccagcgt ctgagagtgc ggtagagacg atagttgtgg attaccacat
    31501 atgtaaacag cagatccgcc acgatcagaa cgatgttgag cgtgaaggtc tcccaggccc
    31561 catagaacga gcctgccagg atcaggatgg ccagcagccc ggctttaaaa aacacgatcc
    31621 cgtgcggcac gcccagtttg cgaaagaccc agcgggcgat gggattcttt tcccggtaaa
    31681 agtgccgcgg gcggatcacc agccaagtgg aatgaccgtc caatacattg agcagcagga
    31741 agataattgc cagcaccagc atcagtaggg tggagttgag cagggtgtac agagtgctca
    31801 cttttttccg tgctcctctt tgtctggaat gggattttgc ctggccgaat cggaagtctg
    31861 ccgttcggct ggatggggtt cctggcctct gggatgggcg gtctgatagg ctttctttac
    31921 gtcctccagg gtcacatggg tatagatctc ggtggtggag aggttgctgt ggcccagcat
    31981 ttcctgaatg gctcgcagat cggccccccg ttccaaaaga tgggtggcaa agctgtgtct
    32041 gagggtgtgc ggggaaaagc ctttttgatt ggcaatcagc ttgatgtagc cctgaagcag
    32101 aacgtatatc tgcctgctgt ccagagcctt gccggttctg gtgagaaaca ggatatcccc
    32161 gctgtgagcg ctcagcagct gctgccggac cggcagatat gcccggatcg cttccagagc
    32221 gcttttccct accgggacga tgcgttgctt gtctcccttg ccggtaactc tcaccactcc
    32281 gcgtttcaga tcgaggtcct gcatcctgat cccagccagt tccgcagccc taagtcccga
    32341 tgaatagagc agttccatca tggcgcggtt gcggatgcca aagatgttgt ccatatccgg
    32401 gattctgagc agctgttcca tctcctcttc agtgaagaag ttgggaagtt tgcgcccgta
    32461 tttggggcgt ttgatcttgt ccatgggatt gaccgcgatc cgctcatccc gtttgagcca
    32521 cagaaagaag gtgttgagcg ctgaggtttt gcgggaaagt gacctgttgc catcagggcg
    32581 ctcatgcagc caacgcagcc agctcctgat gtggataacg ccgatgttat cagccctggg
    32641 ctcggaggat ggatcaagtt ccagaaggtg ctcacgcagc tgttccagat cgctcctgta
    32701 ggctttgacg gtgagcgctg atttcccttc cagcctcagg aaagcgcacc agcggtcgat
    32761 gtcttccagc atggcattac tccctgtttt tgtgatgggc gcattttttg cttgcctgaa
    32821 tacttgtcga gaaaaaagtt ggtattagaa taaggggagg ttttatgaac cgctacgtat
    32881 cggacagcgc caagctcggc cagaatgtga gcctgggcat caatgccgtg atcatggatg
    32941 acgtggtcat cggagataac tgcttgatcg gcaacaatgt agtgatccat gccggcagcc
    33001 ggatcgggga agcggtgagg atagacgaca acaccgtgat cggcaaaaaa ccgctgtcct
    33061 caccccgcag catttttaag gttcccagtg atctgaagcc tgccgtgatc ggttcctttt
    33121 gccagatcgg cgccaatgtg gtgatctatt gccagtgcga gatcggccag cgcaatctga
    33181 tcgcggacct ggccaccgtg agagaaaacg tcagcatcgg cgagctcaac atcattggcc
    33241 gcaatgtggc gatcgaaaac tatgtgagca tcggcaaccg caacaagttt gagaccaatg
    33301 cctatataac cgcctattcc aagattggcg attactgctt tgtggccccg ggcgtgatca
    33361 ccagcaacga caattacatg gcccgcgatc ctgagcgctt caatcacttc aaagggatca
    33421 cgatggaaga cggtgcccgc attggtgcca acgctaccat tctgccaggc aaggtcttac
    33481 acacagacgc ctgcgtggcg gccggtgctg ttgtaacgcg agacgcccaa gccgcgctga
    33541 tcgtggtggg caaccccgcc aaagcttttc gggatgtccc ggaggctcaa ctgataaaga
    33601 acaacctgga taaaaagtga tgaaagcgat agtcatcatc cccacctaca acgagatcga
    33661 aaacatcggc aggatgctga atcaggtgct gacagagcat cccgatctgg aaatcctggt
    33721 gatcgacgac aactccccag acggcaccgc caaagacgtc aaacagatca tggccaccca
    33781 gaccagggtg cacctcatcg agcgctccgg aaagctgggg ctcggctctg cctatgtgac
    33841 cgggttccgc tacgccctgg ccaacggttt tgactatatc atggaaatgg atgccgattt
    33901 ctctcataat cccgatgatc tgccccgctt gctggaagaa gcccagaaac gcgatctggt
    33961 gatcggctcc cgttactgca acggggtcaa catcatcaac tggcccttcc ggcgtctgct
    34021 gatcagctat ttcgcctcca aatacgtgcg tgtgatcaca gggatgccgg tcaaagatcc
    34081 cactggcggt tttaaatgct tccaccgcag agtgttggag agcatagacc tggatcgcat
    34141 cctctccgat ggctatgctt tccagattga gatgaacttc cgggcctggg tgaaaggctt
    34201 ccgcatcaaa gagataccca tcatcttcac agagcgcatc aacggggtct ccaagatgtc
    34261 ccgccgtatc gtgtgggaag cggcctggat ggtgtggcag ctccactttc tcaaactgct
    34321 gaaaatgatc aaatgaggag accactatga aacgcctgat cccctttatc ctgctcttga
    34381 tcttcacctt accactgatg gcacaaccca atttgcccag gatctatgtg cagaaactca
    34441 ccctggacaa tggtaagctt ccctttgtga cctggctgga caaagtgagc gctccggagt
    34501 atatcctcga ggcctggatc aaagaaaaac ccggtgatct gatgagcact gaaactcatt
    34561 cctaccacca tctggctgtc gctcagatcg gagacgggat caaaatgccc tttaccctcg
    34621 tcgccagagt gcagcttggc aacttcaaat atcagtggga atcgggagaa actctccacc
    34681 tcaagctgac ccataaggcc acgaacacca tcaaggaatg ggatgtcctg gtcccagagg
    34741 gctcggcact gatcagatat atggataatc ccatagtcat acctccctac acagaatccc
    34801 gatagctatg ctggaacgca tcaacgatcc catcgaacac ggccccgatc aggaatttga
    34861 ccgtgctctc aggcccaaaa ctctcaacga gttcatcggc caaaaccacc tcaagcagct
    34921 attggacatt tccatcaagg ccgccaaact gagaaaagaa cccctcgatc atgtgctctt
    34981 ttatggtcct cccggtttgg gtaaaaccac gctggccagc attatagcac gcgaactggg
    35041 ggtcagcatc accgtctcca gcgggcccgt gatggaaaag cccagcgatc tggcaggcat
    35101 cctcaccaac ctacagcgcc atgaagtgct cttcatagac gaaatccatc gcctctcaca
    35161 cgtgatcgaa gagtatatct accccgccat tgaagacttt gagatggaaa tcattctgga
    35221 cagcggtccc agcgcccgca ccctcaaaat cgacatcgag cccttcactc tgatcggtgc
    35281 taccaccaga gcgggactct tgacccctcc cctcagagac cgcttcggca tcgttctgag
    35341 gctggattat tacgattttg actccatctg ccagatcatc aaacgcagcg ccagaatcct
    35401 ggacgtgcgt gccgatgacg aaggcatcca tgaaatggcg cgccgcagtc gtggcactcc
    35461 ccgcatcgcc aaccgcctct tgagaagggt gagagactac gcccagatca tgggtgacgg
    35521 catcatcact ctggaaatag ctcaacaggc tttggaaatg ctgcaggtgg accatgccgg
    35581 tctggatgag atggataaac gcctgctgct cactatcatg gaaaactacc ggggagggcc
    35641 tgtcgggatt aaaactctgg ctaccgccat cggagaagac gctggaacga tcgaagaaat
    35701 ctttgagccc tatctggtcc agcaaggctt tctggaccgc actccgcagg gcagaagagc
    35761 cactcacaaa gcttatgccc acctgggtgt caagcctcag gactcccaaa ccgagatctt
    35821 ctgatctcca tatcatggag ccgcaagcgt cctcgttacc ttgtggattc gcaaacgtcc
    35881 tcgtttgcgt tctgtgacct tcagacccct caatgagcta taaggccaac atcgccagca
    35941 acctcatcaa ccagctactg aggctggtgc tgggactcgc gacttccatt ctggtagccc
    36001 gcgctctggg tcctgaaggc cagggctatg tggcctatat cgttttgatc ttcaccttga
    36061 tcggttccta cggccatttt ggtctcaaca acgcggtgat gtatttccac aagcgtaaag
    36121 gcatcgatgc cagccacctc tatcacgtga acagcacctg cctctttatc atctttatag
    36181 cattgagcgg cttgactttg ggcgcctggc acaatggcct ctggctgagg gattatgctc
    36241 tggtctgggt gctgggaggc ctgctctttg tgctggcgga attctattac aacaaccact
    36301 atgcctggtt tatcgcctcg gaacgaatta gagaatccaa ccgggtgaat ctcctcgtcc
    36361 tggttcttaa gtctctgttt gttatggctt tctggctcct cggaacgctt gggacgggcc
    36421 attggttttg gatcacggtg ctggccctct gcctcaacgc catcctcttg cagatcaggc
    36481 tgaaacgagg ttttggcttc aagtgggata cctcgctgct gaaagatgaa tttgcctatg
    36541 gagggattgt gtttctggcc gcggccttcg atcatctgca tctgagggtc gatcagctct
    36601 ttattaaaaa catgctcggc atcccgcaac tgggcgtcta tagtatggcg gtgaatctca
    36661 gcgaattgat gttcttgatc cccttgagca tcaataccgc cctcactggc aggttgtata
    36721 actcagagga aggcgacagc ggcagaatgg tgacggcccg caccctcaag ctcacccttt
    36781 atgtgtgcat catactgtcc atcataggga tacctttgtc gttcgccatc ccctggatct
    36841 atggagaagg cttccgggga gcaattctcc ccacgataat cttactggtg ggagtcatct
    36901 ttgcctcgat tgccagggta gcggccccct atttcttcac ctcgggaagg ccaaaggtgc
    36961 atctcagggt cactttgctg agcctcttgc tcaattgcct gctcaattgg ctgctgatac
    37021 ccctctgggg catcagggga gcggcgatcg cctcagccat atcttatttc ttttatggta
    37081 tttactatct gtctgtcctg gtcgtgaaag agaagtttca gctccgggac tttctgagat
    37141 tgaggatgtc agacataaac gctgtgttga ggaaaccatg aatatcatct ttgccaccaa
    37201 acgtcatcct gcctggcact accgtggcca ggtccacacc cgcggccacg cattttctcc
    37261 ggatggcagt ctgcacgagg gagaagatct ctgccgcaag ttttcgcagg tgaaaagcct
    37321 gagctcgttt cagaaagtac tggaaggcct ggacggcagc ttttgcgtga tcatcgctgc
    37381 cgccaatcag atcttcgcag cagtggaccg catgggctct atgccgctgt tctggctgga
    37441 gaatgaaacc cataagctgc ttaccgacga tcccgaagcc gccctgaacg cggtcccctg
    37501 caaagaagtg gaccatctca gcctgacgga gtatttgctc accagttacg ccaccgggcc
    37561 aaacactctc cagaaggggc tttacatcct cccggccgga cagattttgc attatgccag
    37621 agaaacagac cagatcagcc gttctgagta ttaccgctat gagcatgtgt cagcagagct
    37681 tggcaccgac gcggagctgt ttaaacagct ggacctcatc catcagaatt gtgtgaagcg
    37741 cctcatccag agcgctgatg gacgtcccat cgccatccct ttgagtggag gctacgattc
    37801 cagaatggtg gctttgatgc tcaaacgctt gggttataag aatgttagcg ctttctgcta
    37861 tggtgatccc agatcaaatg aaacctccat ttccaaagcg gtggcccaat atctggaaat
    37921 cccctggacc tttgtggaac accgtcccag ggactggtat ctggcctatc aatccccttt
    37981 gcgccaggat ttttaccgct tcgcggggca cttcagcagc cgtcctcata ttcaggattg
    38041 gctggcagtc agagatttac agcatcggaa ccactggcat ccagagacta tctttgtgcc
    38101 gggacactca ggtgacttca tagaggggtc ctacatccca cctgcctttg tggagaggaa
    38161 caccgtctcc aaagacctct tcctggatca cgccatccag cgtcattaca ggcagtggcc
    38221 ttatgatccc agagcgggtg agcaggccaa cgcgatccgg acaaaaatcg cggagcaact
    38281 caagctgccg gaaaagatga gctccgaaga ggctgccagc cattttgaaa ccttctactg
    38341 gctggaacag cagcccaagt ttattctcaa ctccatgcgc gtctatgagt tctttggctt
    38401 ggagtggcga cttccctggt gggataagga actgctggag tattggaaga gaatccccat
    38461 ggccaaacgg gccggacggg agttttacaa acgctatgta agcgccctcc gcgataccgg
    38521 tttcccggtc tataaaaagg aaagcctcat ccgcagaatg gaagatcgcg tcatccgtca
    38581 gcgctgggga tatctctacg aatcccgctg gagccgcttc gccgatttga ccaaagacaa
    38641 agaatccaga tccacaacgg tccagggttt attgccccag ggtctggatt tgcccgcctt
    38701 cgtgaacccc aaacagagga ttgtggatac cgatatcaat ggcctgcagt cactggtggc
    38761 aatcaaggag tggtgggagg aaggccatag ggtttaaagg ttttaaggtt ttagggatga
    38821 gaggcgcaga gagaactacg ccttgcctcc ataaaactca aaaactataa aactccaact
    38881 caaccttctc aacttcctca accgaatcca ggccacctgt tgttagatcc cgctgatcaa
    38941 tagtattcat agctaaacat cttgtgctca tcatcttccc catccgagtg ggacctctga
    39001 acaaggatat tgccggtgga atcaagttca tatgcatagc tcttgacgtc tgtgctttga
    39061 taggctggga aacaccagct ttccgtgata agcttgccgt ccggatcgta ttcgcattcc
    39121 agctttgaga tcaaatgctc ttctgcgtca tagacggagc gctccaacac cttacccaga
    39181 ttgtttttac gggttacgac cttctcctgg gccacacctt cctgatagcc tgaggattcg
    39241 atggtgattg attggtccgg gatataggca taggtatagc gttccgagtg gcctttggaa
    39301 atatcaaata ccgtcctggc tgtgacctca cccctgctat tgaacagata cacagtaata
    39361 tagctgtctt cttccgaatc ctgcaagttc tgagagatga ggctgtaaac agagtcggcg
    39421 tattcataac tgtgcgtcac cagggcctcg ccgctggcac taagctcttt tactatcagg
    39481 ggatagcctt gccgatcgaa ttcgtattgg taaaggatga cttcaggctc acttgccacg
    39541 ccatcgatgc tcgtattggc cagacttttt atcatccagc cctgggcgtc ataccagaag
    39601 ctggtctcgt agatatgtcc agcgctgtaa agaacactcc tcagcagctt tccgtcttcc
    39661 gtatattcac gggtttgata ctcgtcaggg ttgccgtctg tcctgatggt gtagctcttc
    39721 acccgcccct tcagatcgtg gctttgccat gtgaccggaa ggtatggatt gggaaccgga
    39781 tagctcctga ccattgtctc ggcatgcatg cagcctatga ctgacagcaa cgcggtaagc
    39841 agtgcagttc tgagcatggg ttctccttta ctttttagtt atgatcttta cctaagattc
    39901 aatctttttg tcaaggcaga tctcacattg acatgaagaa tggggctctt tctcttctca
    39961 gtaattgtca atcaatgcag tagggtattg gtatcgaccg atataattca aaccaacact
    40021 catatctatc cctgcctgtt gcttgccgtg gagataccgt gcgtcctccg gcgaaaaccg
    40081 cgggagagcg cacggcatct ctacggcgga tgattgagca ggaatcagcc cccgaaagct
    40141 ggggcagcat atctatccgc tccgatggga aaaatagctt gaccttttcc tgaaggctgg
    40201 cgatgctgct ctcagtctgt aactatatct ttttacaagg agatggacat gaagaaagcc
    40261 atgatgacct tggtctttat catgctgctg gtgggtctgg gcgctcaata tgtgcctgtg
    40321 gagattgtca atcaagccga agatcgcaac ggcaaagccc tggtaagcaa attccgcgag
    40381 atcatcagaa attccagcgc ttaccagatc agctacgact ctgaatctcc ccattttgtg
    40441 gtccagatca acactatgga ccgctacaag ggagacagcg aatgggaagg agtatccaca
    40501 atatatcact atatgatcct catcgacccc ggcgatcaga tgcgggtctt atgcaccaat
    40561 caacttggat acgtaggcgc gaatttgctg gatcaggttg cctatggaat ctacagtgat
    40621 ctggatgatt ttgtggagat ggtgagatat tacatagaaa actgaatgta acgatctaac
    40681 atcctgtcag acgaataaat acaaaggaga taccctatga aacaactgct ctttctgttg
    40741 gcagcgctgc tgctggtcag tggctgcgct cagacccggc tggtaagctg ggaaacgcgc
    40801 aaagtggatg tcccgggcca gaaaaacaac atcctggacc tggctaccgg aaccgtgaac
    40861 accgcgattc aatccagaga atatctgttg ctttacaacg ccgtttacaa ccacgggatt
    40921 cccgccaaca tcctggcaaa aatcaacaag aacgaccgcg tcgcggtgct ggctgctgaa
    40981 cgtgataccc ttgagtatgc agactttatg catctgtttg tgaacgccat gaaccgaaac
    41041 ctgatcaatg ccggttatca ggtggtggac ttacgccctg tgagcggaaa atttggtttg
    41101 gaaccagaac ggctcttcgc ttcgatcgac aaactcctga tcttcactat ctgggaagcg
    41161 ggctcccgca gtgtgatttt tgacgagaaa gccagcgttt tctccggatt tcagatgagc
    41221 ctggacgtgg tggaagccaa aaacaaccag tttctggcca gcagtcctgt ctttggaagc
    41281 gcgcgcaacg attttgacaa gaatgagttc gatcgtttcc agctcttcac gctcagcgaa
    41341 gtgaacgctt ctctaccact gatctatgca gataccagca aaaaaggtgt gatcgcgctg
    41401 gcagactctc ctcagggccg cgtattcatg ctcacggtct ataatcccga gcagatgaag
    41461 ctgatgatga agatcacaga catcagcggt aacaatatca tagaaaaaga gataagcttt
    41521 ggagacagca gttcaggtgg ctattattcc tatgtctggg atggcaaact gatcgacgga
    41581 acagacatcc aggccggtga ctatttgctg tatatcaaat ctgccgatgg agttacgatc
    41641 ggctatagga gtttcagggt agattgagct attgagaacc tgccaaaaat ccggttgagc
    41701 aggcggcctg gatgttgaag cctccggtag ggaggttata gtccagtact tcgccagcga
    41761 agtacaggcc tggcaccagg caggaactca tatccctggc ccgcacctct cccaaatcga
    41821 tgccccccgc gctcagcatg gcagtactga ggcactccac acttttgagc tttaggtccg
    41881 aagagctcag gctgaagccc agagacttca attcgggagc ggaaacatcg gcgcagcgct
    41941 tgtctggatt gactttggcc atcgtgatca aaacaagcag caaggcttcc ggaagccccg
    42001 tggtcttgag cgcgtttttg agactggact tgggacgcga ggccatgatc tgtctgagtt
    42061 tcacggcagc atcctccagg tagcaaagcc tgattgtatc cccctgggcc agcagatgcg
    42121 aatggtccag gaccagaggt ccggataagc cgcgatgagt gaagagcaga tcgccagaga
    42181 ccctgtgttt accagcttga gtgagcaatt ccgctttgat cccgttgagg gcgattccag
    42241 cgcaagtcct gaagggataa gcatctatca ggatggaggc gagggctggt ctgggctcga
    42301 tgagcttgtg gccgagttgt ctggcaagct cataaccgct tccatctgag ccggtttgag
    42361 gccaagacgc tccccccgtg gcgattagca gtttacggca tcggtagagg cgctgatgat
    42421 tgtccgtcag gacaaagccc ttgtcgatgc ttacatgaga gattctggcc gaggagatga
    42481 tctggttgcc gggtttgaga gctccccgca gcaatgccgc aagcacgtct ttggcatcca
    42541 gggaagccgg aaagaccttg ccgtccggcc tcacgatcga ctggcatccc aggctgttca
    42601 gatacccgat cagctttgcc gggctgagtt tatagagggc gggtttcaaa aatctggcat
    42661 agcttccgca acgcttgaga aactcgtcca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42781 nnnnnnnnnn agggcgggtt tcaaaaatct ggcatagctt ccgcaacgct tgagaaactc
    42841 gtccagctcg agatcatggg tgaaattgca ctgtccggag ccgcttagca acagctttct
    42901 gccaggccgc ggacccgctt ccaggatcag acaggtccct gttgaagcgc tggcggccat
    42961 caatcctgcc ggaccggcgc caatcacgat atttggatac tctggattca ctttttgagc
    43021 atggccttgg tgaaggtggc agccggaatc ttgaggtcga aagcaatcgc gtccacatag
    43081 tattccgttc ccttgccgtc ttttagctcg tccttaaaga ggatatagcg gggataccag
    43141 cgggacccga cgcggccgac atttccggct ttgatggtct tgagcagctt accgctcttg
    43201 gcatacatgt attggtagag caccacatag cgttctttgt ccacatagag cttgagtttg
    43261 tggtaggcga ggtttttctg tttggccacc agctcaagca cccacacctc gcgaccgtcg
    43321 tacttgctgt ctccggtcac ggtggcagtg taggattctt ccagcttggt ctcttccata
    43381 aagtcttcat aggagaggtc cgaacccatc acagactgct tgagcatgtt tcccgagatc
    43441 tggatggtgc ggtcgctgga gggctcatag atccagagtt tatcgcccac cttgagcatc
    43501 ttggtgccct tttctctggg aggtgcggtg tattctgaat agtatttgtc gctgccgctg
    43561 gagtagttga tcgattccac cgtgcgtgaa ctgcgtttgg agtgaatcac catgcgggtg
    43621 gtggatttgg aagtggaaga cagcatattt ttatccaccg ctttgaggat ggaaccagca
    43681 ctgggagctt tctgagcgca cagcagggcg ctgaagacaa gcaggcagaa tatgtacagg
    43741 gcttttttca tgactcgagt tccttaaaga gttgtgaggt ctgccgtctg aaaatgggga
    43801 tgcccgctaa agcggccccc accagagtcg acatcactcc tgtgatcaat ccaatgagga
    43861 gggtctggag atttaacgcg gtgtagatca tgctttcaga tacgatggaa gagctgcggg
    43921 cataggcgga tacgtctatt ccgtttctgg caaagagcag cgagatcgct ccacctgcca
    43981 ggatccccaa cagcgatccc gcgatgccaa gcaccagagc ttcacccacc agactgccat
    44041 aggcgtggat tttgcgctct cccacagcca atctgaggcc aaattccccc caccttctga
    44101 tgccgttcat caatccggaa ttccataaca cgatacccag tatgatgata aacaccaggc
    44161 tcatgatccc cagcgagctc ttcatgatcg ccatgaaata ggccagattg ccctggtctg
    44221 tgagggccag catttccacg tcaaattcct ccggaccgga gaaccgggaa ttgaaatccg
    44281 ctttcagctt gttgacctca gatgctttgt aattgccgtg tttgaagaaa gccagcactt
    44341 cactggcccc accttccaga tcaagcattg tcctcacatc tgagatatcc gccaccactg
    44401 caccccggtc cagcgtgtgc actccaaaat ccaccgatcc cactaccttg agattatgca
    44461 tactcatggc gccatggacg gtggaactga tcagagtgat ggggtcaccc agccgcagcc
    44521 tgagtttgtg gaacgcggtc ctgctgatca gcacttcgcc tttctggcgt ggaagcctgc
    44581 catccaccag agctttattg aggttgaggc ggtcgcgttc cgcaggattg ttgatgatgt
    44641 cagccgcgaa gcctgccact tcaccctgct ctctggtttc acccagtgag tccgggacgt
    44701 ccaggagcgc tccaaagctg attctctgca cccagtccag ctgcggatag gcttgtttcc
    44761 atctggccag ttcaggttca acatccagca tggcgaggtc gaaaggcttg aggctgagct
    44821 gtttggcata agctttggta accactttga gatgcccggt ctcaaatctg gcattatgac
    44881 gcacaaagct gaccgtgaag ccctccataa aggccagccc aaagaccagg atagcgatcc
    44941 cggaagctac aataatgagc ggaaacaggt aacgatgcct gtcccggaag attcctttga
    45001 ggataaactt cagcatctca gaccaccttt ccgcgcagag cgtctgtggc cttcattctg
    45061 gcaatccgcc tggccgggat ccaacttacc actacagaca ccagcaccac aaagctgaag
    45121 acctgcagca gcatcagagg cggatagctc attttgatag gttccatgaa acccagaatc
    45181 ccaaagctgt catagccttc aggcatgatg tatcccgtgg tggcaaaata ccagaacatg
    45241 ggggtgccca aaatcgccgt catcccaatg gcaaacaggg aatacagacc gccttccagc
    45301 gtgaacatag tggttatctg ccgctgtgac atccccaatg cggtgagcgt gccgatttct
    45361 ttgcggcgct tgaacacagc cagggcctga gtatcaaaga tggcaatcat cgccagaaac
    45421 atcaggaggc tgtaagtgat atatttctgc accgtctcag tcttcatcat ctcatccaga
    45481 tcgaagaaca gcgctttctc gtccatgtaa tggaatcccg gcagggacag cacccccagg
    45541 ctgggatcct gcagcgcgat cgccgtcacc atagagggag cttgtttgag ctcttgtaag
    45601 gtgctcaggt ccatccagat aatgccaccg tccacagtcg gcacaggagt gttcatgatg
    45661 tgcacaattt ccaggtcgag ggtgttgaag gcattcccga catccttgat cctgagcgta
    45721 aggatatccc cctcttccag ttgagagctt ttggccatag tctggccaat gatggcagga
    45781 gtgtaacctg gagtcctttc caccagcctg gagctgggga tattcaacag tgtctggtct
    45841 gccgggatgc ccttgaccat gcagggaatc attctgccgc cgggatagat gaccccggga
    45901 gaaaacaaga tgggcacggc ttcctgcctt tctattacag gccgcaactc atccggaaga
    45961 ggctcaaagc tcttgtccca cgagaacgcg tcaaagggat catagctgtt gacacggaac
    46021 atccctttgc caaattccca ctctttttgc tgggttttgg ccagcagcgt ccagctgtgg
    46081 tacatgctct ccataaagat catgccgatc atcacgatgg cgatgataaa cacgttgatc
    46141 aggctgcgcc agccgtttcc gatgatgttt ctaagcgcca gtctgaagat catcggccgc
    46201 caccctgcca tcaacgagtt tgatcactct ggtcaaaaaa tcaatcacct tctggtcatg
    46261 tgtgctgaag ataaaggaag ttcccgtctt gagattgatt tcccgcatgg tcttcaggat
    46321 gtgcagggag ttttcggtat ccagattggc cgtgggctca tccgccagca ccagtgaggg
    46381 ctttttgacc atggccctgg cgatcgccac cctctgacac tgacctcccg atagctgagc
    46441 gggacggctc ttgatcttgt ccgtaagccc cacccattcc agagcttcat tcaccagctt
    46501 gcgccgttcc tctgaattga gcttgagcag cagcaatggg aactccacat tttcaaacac
    46561 gttatagact ggcagcaggt tgtaggtctg aaatataaag ccgatgtgct ggctgcgaag
    46621 ctcagcagat tggcgggcgc tgagtttgct caccttgtgg cccaggactt ccacttctcc
    46681 ggagctggga acgtccaggg agcctatcac attgagcagg gtggttttac cactgccact
    46741 gggacccacg atcccggtaa actcaccttt tccgaactca aggtcgatat ttttgagggc
    46801 caggaattct ccctcaccag tgggaaaggc tttgctcatg cccttcacgg tcactatcag
    46861 ttcttgcata catcctcgct tatctgggac cgccaaggtc cccgcttaca tattatggtt
    46921 tggcctgaag ttctatgagt gtgtttaaat atcataactg actttcagat agagatatgg
    46981 agagcgggag agctgatctg gcatgcccgt atcgagggcc aggcccagat cgaaggagag
    47041 ataatcgtaa gcccttctgt aaagcgcgct cagattgcca ctttcgtctg tccaatgcca
    47101 agatcccagg atagtcacgc cgtccagcag tcccagtgga tagtttgcca tcaaagcgct
    47161 gacactggct tgattatgca ggctttccgg atcgtttgag ccagccagga tcatgtgttc
    47221 cagcgtaaag gcaaggccat tagctatccc gacggtgtaa tcgcctccca gagtagcgga
    47281 tagctggtat ctttcgaacc cgtgagagac attctcatac aatgaaccag agctttccag
    47341 ccacaagccc ataaagctgt cgaggcgatg atcaaggccc aggcgatatt ctctgccctg
    47401 aatgagctct cgctggtgat aactgatggc agtttctccc cagcgactgg gcagttgtaa
    47461 tctgcctccc agctcaatcc tatcatccct ccctgagagc agttccatac cttttagatc
    47521 atcggaaccc ggcatggccc atagccataa gttggcatta tttagccagt tatgcctgat
    47581 caacagagct ttgacgccct gggtttcctg attggcatcg agcggatcga gagtgtcaaa
    47641 ccacctcagg gggcgcaaga tcatggcaga tccaaagtta agcctctgca ggcctgccag
    47701 cacctgggtt tgatctgtgg ccagtctcaa ccaccagcga tagctgtcca gcttgatttc
    47761 agcagtgtaa gagccttcct gccagttgga tagcagacga ttttgcatgc tgtattcacc
    47821 gctgagttcc agtttgtcca gcagcggata gctgtagcct agctgaggtt gccaattgag
    47881 ctccagccag ctgtcctgat gcttttggtc gcgctccatg ctgaggctga agtggttctt
    47941 cagctcaaag cggtcttccg ctccgatcct gcttagcgcg aggaccagca ggcagacaca
    48001 agtggctcga agtctcataa tctcattcaa ctatctttgg ttttccgatc ctggtgatca
    48061 cgttatctga cagagcaaaa tccgtcaagc tttgatcatc agcttcgctg tttctgctgt
    48121 gacgcttgcc agcagccagg tttacttctt gcggccgcgt gctttggatg gcttgagggg
    48181 atccggcgtg atttcttcga taggagagct ttcttctttg gtttcaggag agctgaactg
    48241 catcggctct gtgatcggat ggcgccgtag ctctaccttc acgataggct cgaggcgatg
    48301 ggtgactaca aagagattct tgtttctgga gagggccagc ttgtcttcag cgctcaaaga
    48361 ttcgatctca gctaaggtag taacgcgctc tatggcgccg gtggccatgt ccacgatctg
    48421 gacaaattgc tgtttttcgg ccatgaagga tacctccatt tggtatttat cagggaattt
    48481 tcttacaata tcaaccacaa aatccacgta ttctttttta tgggaacgat accaggcccg
    48541 gaaagaccgg caacgttcga tgcgtggaca aacagcgcag aggggtgggg tcacctctcg
    48601 gtagcttttg agctcgcagg caaagatacg ggattttctc atgtcagttc aatcacctgt
    48661 atgaggattc tttgtcaagc gatatcgtga aaatgctccc cgggaactca agaaggaaca
    48721 agggctggtg tggtaatgaa acattgagtt caatcgctct gcccggaaat tggggttgac
    48781 cgttttagcg gttcccggaa taatgccctg aaatgaacaa agcatagagg taaaggtgct
    48841 actatcagac tttttggacc cccgcaccat cttgatggaa agcaggattc tgacccgtga
    48901 acaggtctat gaaaacatga tgcgcaagct ctgcgaaacg cataaacacg atctgcccat
    48961 ctgcggcatg ccgttattgg aaaaaatcct ggaacgagac cgggaggctt ccacggctta
    49021 tccatccgga atcgccatcc cccacatccg tttggacgga tttcaggaca cccttatctc
    49081 gctgtgtttt atgcaaaacc ccctggattt cgacggcacc atggtaagct gggtagccct
    49141 cataatcacc gataaatctt cttccaggct ctatctaaac atcgtggccg aacttttggg
    49201 actttccaaa aaccaggcat tgatggagcg tcttcttagg gaaagagatg gagtggggat
    49261 caagcatgtg atcgccaaag aggggatcag agtgagcaag gatatcttcc tgagcgatat
    49321 tatgatctcc gatcccgttg tcgtacatcc ggataccacc ctcaaagagc tcaacgccct
    49381 gatgaacacc cacaacatcg ccggattccc tgttgtggac gaaaagggaa gattcaaggg
    49441 tgaagtcaac atcctcaatc tgctgaaagt aggagttccg gactatttgc tgatgctgga
    49501 gaatctggct tttctgcagt cctatgagcc tctggagaag atatttgaac aggaagaatc
    49561 catcctggtg aaagacctta tggataaaga cgttatcacc ttaaattcaa acgcttccat
    49621 catcgaagca gtctatgaaa tgatcctgca caagaaacgc tatttcagcg tggtggacca
    49681 gggtcagttg aaaggggtga taacagccat ggatattttc cgcaaagtga taaaggcctg
    49741 atagatatgt ttttaatgct acttgccctg gtgatctttg tgctcaccta cgtcttgatc
    49801 atcacagagt ggatgaataa aatgctggcc gccatgatcg ggggattcat gatcgtcgtg
    49861 accgggatta tgcaccagaa agaagctctc atggctgtcg actggaacgt gattttcttt
    49921 ttaatcggca tgatgtttgt gatttcggtg ttgaaaaaaa ccggcgtctt tatgtatatg
    49981 gctatccgca cagccaagat cgccaagggt aaaccgctgt ggatcatgat attaatgttt
    50041 ctgatcacgg cctttctgtc ggccattttg ggaagtgtga ccacgatcat gatcctggtg
    50101 ccgattgtcc tactgatctg cggagagctc aagatcgctc ctatcccctt tattatcacc
    50161 atggtgattg cctccaacat gggcggcgcc gcaaccatgg tgggtgaccc tcccaacatc
    50221 ctgatcggta gctccacttc ctaccaattc ctggattttc tcttcaatct cacccctccg
    50281 gtgatgctca tcaccgccag cagcattggc atcatctggc tgatgtatcg ccataaaatg
    50341 agggtgagca atgaaaaccg ggcccgcctc atgagctatc gggaagattc cctgatcaaa
    50401 gaccgcccgc tgctaatccg cagcggaatc gtgctgctgc tcatgctgac cgccctggcc
    50461 atgcaaggca ctctgggagt ggaaacggcc actatctcga tgagcgcggg actgctgctg
    50521 ctcatcctca gtgaccggaa aaaagtcgaa catgtgctga tgaacgacgt cgattgggtg
    50581 accatcttct tttttatcgg actcttcatg atcgtggaaa gtctggtcaa gaccggtttt
    50641 atcgggatgg cggccaagca gatcataatc gccactcatg gtgagcctaa agcagcttcc
    50701 atgatgattt tatggctttc cggagtcttt tccgccttta tagacaatgt accttttgtg
    50761 gccgcgatga ttccaatggt aaagaccatg ggcaccatga tcaacaatcc cagcgccatg
    50821 caccccatct ggtggtcact ggcattggga acctgcctgg gaggcaacgg cacactgatc
    50881 ggagcttcgg caaacatcgt ggccgtcggc atcgccaacc gcaacggctt ccatatcagt
    50941 ttcaagcaat tcaccatcat cgggatgatc ttcacgctca attcgctgct catctcggct
    51001 atctatatcc tgctcagata tttttgatct ggccttaccg ccagacaagc ccctccccta
    51061 cagagatatc cgcagattca gagatgcctt atgagctgac caaacttgtc aactccgccc
    51121 caaggtccta tgtcgctact ttcctatcac ccactcctct cgtttactcc tcctatgccg
    51181 aggcaaagga gaggcaaagg aggggcatag gtggtgtaaa ggaggctgat tggaaggcaa
    51241 aggaagacaa agtgcttgag atcttctgca gcagggttgt ggcactgatg tgattgccag
    51301 tttgaagctt tagctcagaa cagcatcccc aggatgtcgt ccacttccac cagcccaaaa
    51361 tagtcgctaa gctgatggct ggaaagcact tccctcagga tttgctcctg atggggctgg
    51421 ttcagcatgg ccctttcaag ttccgtgctg tccttgttgc cgaagtagtc tccgtaaaat
    51481 ctgatgccgg taatgcggcc atgcttcact tccagataga cttctatggt acccgcggcc
    51541 gtcctgatgc cacgatgttt cgagtaggcg ggcgagtggc caaagttcca atcccagctt
    51601 tcatactttg ctttacttag cgcctggatg gcggctaatt gttcaggtct gaaacggtaa
    51661 tcgcttgagt ccggatagat ttctctgatc cgggcgtgga ccagggtgat aaactcctcc
    51721 agactaagag cttgggaaag atgctcttta acgttggtca cccgggaacg gatagatttg
    51781 acggccttgt ccctgaattt gaggggattg gccttgaggg ctttggagag gtcgttgatg
    51841 cgggaggcaa acaggatggt gccatgctgc agaatgcggc cggcaatgat ggctttggca
    51901 ttgccggaga acttcatccc cttgatggtg aggtcgttgc ggccttccag acgggcttcc
    51961 acccccagcc ggttcagcac ttccaggatg gggcgggtgt atttttcgaa ggtatgaagc
    52021 ttgtcgctgt cgtccgtcac gatgaagctg aaattgaggt ttccgaggtc gtgaaacacc
    52081 gtcccgccac ccgtgagcct tctcaccacc gggatgtggt gctcgcgcac atagtccaga
    52141 ttgacttcag caagggtgtt ttgatgcttg ccaactatga tggacggggc gttgatgtag
    52201 aggaggaata catcttccgg aatgtggtga aggatgtatt cctcagtggc gatattgaag
    52261 ggcgcgtggt gggaatcgct gacgataaag agcatgggcg cgggcctatt tgaggctgtc
    52321 gagttcccgt ttcacaaagt ccaccagctt gctcaggcgc tgttcgctaa aatcaaattc
    52381 gatcccggcg gcctgataaa gttcgctgac aggtttgctg tagcccagct ccagaaagct
    52441 ttgataagct ttgagggcct tgtctttatc ttcacggtag ttcatataga cgctcagcgc
    52501 gcccagctgg gccatgccgt attcgatata ataaaagggc acttcaaaga tgtgcagttg
    52561 ctgcatccac ctgagctgtt tgtagccttc cacaccactc caatcgacat ccgggaagaa
    52621 gcggttgtag atatccagga aagcggcgcg tctctggtcc acgtcatgct ccgggtgggt
    52681 gtaaacccag tgctgaaaag cgtccacgct catgcaccag ggcaaaaagc tgagcgttcc
    52741 ttccagttga tcgcgcttgg ccttgatcag gtcgtcacgg tcctgataat agatatccca
    52801 gtggtccatg ctgaggagtt ccatgctcat ggaggccagt tctgccacct cgctgggagt
    52861 gtccttgtaa aaacagatcg ggatgtcctt ggtggcggcg ctgtgcatgg cgtggccaga
    52921 ttcgtggagc agggtgacga cgtcgttgtg ttgtttgacg tgattcataa agatgaagga
    52981 agcgcccaga tcgttgagag tggtgttgta gccaccggga gctttcccct tgcggttttc
    53041 caggtccagc agaccagtgg cgcgcatacg ggctagctga gtagcgtaat ccggcttcac
    53101 tttctccagc acctgaagag ccttgtcgat aaactcatcc gtggtttcaa agggcttcaa
    53161 gaccttgccg tcgaggtcca cgatcgtgtc ccagggtttc acactttcga ggcccaagcg
    53221 ctgtctgcgt tcttcactca gttgattggt gaaggggatc accactttgg cgacggcgtc
    53281 gtggaagcgg taaaggtcat cgggggtata ggaaaagcgt cccatccact cgtgcatgta
    53341 atcccggtag ttggcgaagc cggtgttggc ggcaatttgt tcgcggagtt tccgcagctg
    53401 gtcgtagagc gtgtcaaatt cagcttcttt atcttgaaat gcctgcattc tgaggcgcca
    53461 ggccgtttcg cgtttctggc gatcggcgtc tttgaaatag actcccaact gggccatggt
    53521 cagttcttta ccctcaaact cggcggtcat cttactcacg attccgccat atttgttggc
    53581 gagctcggat tcctgaatct tgagagggat gttctcttcg cggaatagct cgatctgctt
    53641 ggccagcatg cggttgaggt gtccaaagcg ctcctgggat agctccgcgc gccaagggct
    53701 gtcgtagaaa agcttcttga tccggaagga gaaaggctcc gatggcatcg ccacctgggc
    53761 atagtaatcg ttgtaggcct gttccttgct ctcgtcgtcg gcatggacgg tcatttccac
    53821 atagaggcgg gatagttcat cttccacagc cattgagatc tcggaataat gctccaggac
    53881 ggtgatgagg tcctgagcgg aggagggaac tgatgccgcc agggcttcca ggctgcccag
    53941 cacggtactg gggtcgcgga cttcaaagcc ggctgggagg tatttgggag tttttggagt
    54001 aagaatgtga gtagaattca tctatttcct cttgtatcgt ctcaatttga tgattttgat
    54061 ccacaggatg gataccagcc ccgatgtcag tagaatttcc ctcacgccta cgagggggta
    54121 aaacttcact ttatcgcctt tgatggtata gatgcccacc ggagtggtgg aaatctggcc
    54181 gccgcctcca ccgccttcgt tcacctggga tttggcttcc ggggattctt ccagactttc
    54241 actggtctgc gtttcaagct tcttagaggc ctttttggcg gtaacgggac tgctgcctcc
    54301 cccacctcca aaagccatgc tgatcctggc cacggggatc acagtaacgt cgttgagctg
    54361 ggtggccttc ccaaaagaca ggtctgctcc ggctccttta ctgagagctg aacgaacctg
    54421 agacaaaatc tccagaaact tcatcttcca cctcctgagt ggttcggact aaattgtgag
    54481 atacgctatt cctgtcaatt cacaaaatgg gattacttca ctacccgcgc atagaaatcc
    54541 gctccgctgg cagaaccaaa gtatcccaga gcgttgttga tcccgccata gaagtatcct
    54601 tccggccggt agctgtattc atagtagttg tcgtcgatga tataagcgga gacgcgatag
    54661 cggccataaa aagccactga ggccgcgtag tctggcaaaa ccagatagtt gccatccatg
    54721 ccgggttgtg actttgaggt gtaaatgccc atcacattga tgcgcctgaa gctctggccg
    54781 ttggagttgt agatcggttc cagagattca tccagattgg tgatgccaaa gatgggagtc
    54841 gtccactcca gatccgtgct gaagggttcc cggcaataga tttccgccat gaaattgaaa
    54901 tcgcctgcct gagctccggt gcccaacacg atgggatact gctggtccag aatgctgtat
    54961 tggacagtgt ttgcggtttc ggggtccaaa gaccagccgg tgcccggagg gttgttctga
    55021 tagaaatcca tgacaggggt cacagcctgg ggcacggtgg tctcagccca gatcaattca
    55081 ggcttgccat cgatcctcac ctccagacga tagctttgca ggggctggat gatgcggttt
    55141 tgcgggtcaa catagataaa accccggctg ggatcaaggg cttcttccag ttcccagcgc
    55201 tcattggtct gcagatcgat cactgtgact tccgcgtctt ccacaaagag ctcgaaggga
    55261 tcaaagtctt ccagggagat gctgcgggtc acaaaaatgg gattctcggt gctgatactt
    55321 tttcccgcca ccagcagtcc ggcaacggtg taaacttctc cctcaaagcg gggcttggag
    55381 gtgaattcac aagcgctgag gctgaacagc aggatgatga gcaaggccag atatggcagt
    55441 ttgaccagat attttaccat tttatattaa ctcctatgaa gggtagaagc ggaaactgcg
    55501 cgctgtcact ttccaaaaga ctgatgccgc catcatcttc tgtgaccaga cggtagtttc
    55561 tgaaggccac attcttgcgg ttgaagacgt tgatcacttc cagatagggc tcgatgctgc
    55621 cccagcgggt cacaaactca tatttggtgc tgaggtcgag gcgcaaatag gacggcaggc
    55681 gcacccggtc ctgatagctg tagatgatct ggaagttgct gccgtcgtag tagatccgct
    55741 ctggaacata ctccggctgt ccggaattgt aactgaaatt cattcccagt ttcaggtccg
    55801 cgcccaaaag ctgccagccg gtattggcgc tcacgttgaa ggtctgagcg atggtgagac
    55861 tgtgggtgcg gtcgtatttg ggataaaagt aacggggctc accagtagca ggatcgatgt
    55921 taacctccgg aaatctgcgt ctggtttggc cataagtata gccggtaaag ccctgcaggc
    55981 ctttccagtc gttgcgcacc aagaggtcca ggccgtaggt gtagccctta cccacatgga
    56041 aagtgtcggc caggttgcca gtctcgttgt cccaggtgaa gtccgtcgcg gtattatatt
    56101 gcaggatgtt gtcatagctc ttgtaataga cctcggcgtc cagagcgaag tttgagctga
    56161 tctccttctt atagcccagg atgtaatgta aagacaaacc tggcggcagg cttccatcca
    56221 gcgggaacca gacgtcgaaa ggagtgctga cgtccatgga catgagggtc aggtattgat
    56281 ggtaagcccc aaaatgggca tagacctctt ctcccacatc cagtctgcgg cgcaccgaaa
    56341 cgcgtggctc cacattgaag tatgaggcag cgggaatgtg ggagggtttg gtcctgaggc
    56401 tgcgatacca ggcaagcctc aagccgggtt gcagggtcca aaaagtgttg atgtcccaga
    56461 tatcctgaat gtaggcagac gaggtaagcg aggccacttc cacgtccgga agcgagtttt
    56521 gggagagctg gtaactggtg ttcagtttca gccaggtgtc gttccatttg atctcagcac
    56581 cggcatccac ttcatgctgg ttgttgggtt tccagctcag catggccttg gccgagaggt
    56641 cctggatacc gttttcgcgg gtgaaggtct tctcgccctg caggtccacc tggtcaaaat
    56701 ggctggtgaa ctcactgccc gccagaataa actgggaaaa gagggtggga ttgaagaggt
    56761 ggatccactg agtggtgaag gtgttgttgc cccagtccag agcgagcttg ctgccaaaat
    56821 caaagctgag gtcgtctttc ccaaagtagg cactggcgga aaacttgtcc cgctgcccca
    56881 tatcccagtt gagcttcaca tgcccgtcat agaaatagta ttctggcagc tcttcataga
    56941 acttttggac cagatctaca taggtcctgc ggaaggaagc catgtaagag ccattctgcc
    57001 ccgccacttt ccatggccct tccaaagtag cgctggaaga tatcagactg agccgggcca
    57061 cgccttgatg ccgcaaacgg ttgccctgac ggttgttgac gtccaggaca gaggaaagcc
    57121 ggccaccaaa cttggctggg tagccaccct tgatcaggtc cacgctttcc acggcgtcgg
    57181 tgttgaaggt gctgaaaatc ccgccgaagt gattgggatt atatacatct atatcatcca
    57241 ggaggatgag attctgatct ggagagccac ccctcacata gaggccagcc gagaaatccg
    57301 agataggcgc tactcctggc aaagccagca ccgctctgaa aatatcagat tccacgggcg
    57361 acacgatgct cttgatatcc tccgtggttt gcctgatcag gctggtggcg atctcaggac
    57421 cttcgtattt ggcttccgcc gttccataaa ccaccgtctt ctgcagttcg atggagccgc
    57481 gctccagcct cacattgaga gtgacgtttt cgcctacgga cttcaccgtg aacggcatag
    57541 actggctttt ataggacacc aggctgatct ccagagtgta gctgccgggt ttgtcaagcg
    57601 tgagcacgta atagcctttc ttgttggttt ggcctccgat gcgggtaccg cccacactca
    57661 cgttgacgta ttggagcggc tcatcgctgc cctcccgggt cacgaaaccg ctcacggtgg
    57721 cagagaatag accggagacc aacagcagaa ttgccgttat catagacaat ctgaccatta
    57781 ttacctcaat aagaagagat tagacgagga taaactcaat gcccatctta gagagctata
    57841 cagtaactat cctcaactac ctgacaatct actctaattt tgccctggag caagaatgcc
    57901 ctgcgctatc ttgatctgat ctgctccagc aagcctttat tcatgcccgg caatggacta
    57961 tgtgcttgcg ggcatacctc ttggctgatt tggctatcaa gctttacgcg ccacccggaa
    58021 tcccgtgaaa ttggtgctga gagcagcaag gctgtagaaa cgtctggcca cagtgcagtt
    58081 gctggcatcg ctgttccagc taccacctct catcactctg tgaggtccgc tggcgggacc
    58141 ggtgggatca gtgacatcag cagccggata ctggttgtga tatatgtccc agcagtattc
    58201 ccagaggttt ccgctcatat catacagccc cagttgattg ggcattttgg tgccggcggg
    58261 cttaacaccg tccccgccat tctctacata ccagcccact tcatcgaggt cgttcgagcc
    58321 gctgtaaatc aaatcctggc tgtaagatcc gccccgggcg gcgtacatcc attccatctc
    58381 agtgggcagt ctgtaaccgt tggcattcca atcgcaggcc aacagctcat gattgactga
    58441 atcatcccat ccatccggcc aggaagcagg attcacccct aagttgttgt agttataaca
    58501 gggtgtgaag ccttccagga tgctgcgcag gttgcagtag atcacggcct tgccccagtt
    58561 catcgtgtag gtcacacctt ccacatcgtg agtggtcata gggagctcct gatcgatctc
    58621 aggcatgtcc accatcacca tcacccattc cagttcagtg acttcccgct tgccgatata
    58681 atatggagag agggtcacgt tggagctgcc gttgtgaaag ctaccacctg gcaccagttg
    58741 catttgatct gccaggttga tgatgtaaaa ctcctgcact tccaggctgg gctcccagcc
    58801 gtcctgatag gccttggcct tgatcacagt gtttacaggt acgttgatag gataagtgta
    58861 gagcggagaa gcttccgtga cggctgagcc atccgtggtg tagtggattc tgacgttgcg
    58921 acggcctgag ctgatcttga ccatctgagc ggtggaatag tatccaccgg ccggactgaa
    58981 gatgggatcg ggcaaacgca tagtgaatac cgcctgggtg acttcactgg ggttcttgcc
    59041 agccagaaat gctctggctt tcatcaccat cgaagcggct accgtcagag gagcggtata
    59101 gaggctggaa ttctcgttgg gatcgctgcc gtccagggtg tagcggattt ctgctccagc
    59161 tgtcgcgcat gtgaccgtca ctgtctgagc gctggtataa cttcctccgg gaggatcgat
    59221 ctgaggtgga gcaatcgagg tgagcacgta atccgcgctt gcagtgctgc tggatatcca
    59281 tccttgtttg aatgctttgg ctttgagggt gctggaaacg gccagatgaa caggtccagc
    59341 atacaaagga gaagcgctgt tgggctcaga accgtctgtg gtataacgga ttgacacccc
    59401 tgatgtggga caggagatca gcacatcgac tgatgaatca tatactcctc cggccggaga
    59461 aaactcgcag gctgccacgc tctggtcccc tggattggta gtgttatcct tgcaggcagc
    59521 aagcatcagg agtacgaaag ccagcatcag gaacggtctg aagagagata attgcgtttt
    59581 cttcatagca gcacccccac gctcacgact atcgatctca tcctgaaagc cttacggaga
    59641 ttggcttttc ccaggtcatg caggccggcg gtgtatctga ggtcaaacac aaagccatcc
    59701 ttgccgagac ccaattgaaa gatatccctg aggccaaatc ccagtcccac caccagatct
    59761 gtcttgtagg tcagcagctg ttccgatccg ctttcacgcc tctggcttaa ataagaattg
    59821 gccacatccg cgtcagtctg gctggccagc acaaagtctt cgatgtcggt ctcgagggcc
    59881 ttgatgccac cctcactgct ggatgtattg gagagcacga tcccaaacga cggacccaga
    59941 tagataaacg ctccctgata ctgaccaccc ttctgctcct gagtaagctc ctgttgcagc
    60001 ctgaacagaa tcggcagcgt aatatagtcc accgttctgt ggatggagcc ttccagggtg
    60061 tcggcaaact cggtggccag cagcaaattg ttggtactca gagaccggcc ttcaaagaga
    60121 tgctcatatc tgaagcgttc ccagatcagt tctgcctgca aacctaccga gtctgtcttt
    60181 ttcaccagat tgacacagcc ccacaagccc aggttttggg ctgcgccgga ttcggtttta
    60241 cgggagttga gggtcaaata gcccagggga gcattcacca cccccaccgt ttcgaggtcg
    60301 tagcccagtt cgtaaccccg gtcgtctcca gtcagtttgg atgaaccgaa gccatatttg
    60361 atgccccaat ccaggtcgag agaccataat ccgcccaccg tcagcgaggc tatgatacag
    60421 ataacagcca gcttgcgcat attgactcct atgcttttct aagggtgctg tcaggacgcg
    60481 ggccgggcac aaacagaaac caggctcacg ctaaaacgac atctgaattt cttatcctgt
    60541 actcttcggg attctgtcaa gcggaatctg gaaaatattc cacaaagcgg tagaatgaaa
    60601 tctacacaag cctctcaaga gctcccccca aaaaaaggat tgactaattg gcaagctcgc
    60661 aggattttgg aatactaaca aaataaatgg ggttagccaa tgctgaaagc caaaatttac
    60721 gtgcgactca agcccagcgt ccttgatccc caaggcaaag ccgtgaccgg gtccctcacc
    60781 caattgggat tcgatacggt aatagacacc cgggtgagca aatatatcga gatcactttc
    60841 tctgatgatg atccctctat tgtaaaaccc aaggtggaaa agatctgtga tacccttttg
    60901 gccaatccca ataccgaaca ttacagcttt gagctggaaa aggtggaccc ggcttgagag
    60961 taagcgtagt caccttccct ggttccaatt gcgatcacga tgcccgcgac gtcttcaggc
    61021 tcagaggcca tgacagccgt ctgatctggc acaaggaccg cgatctgcaa gaccctgacc
    61081 tggtggtact tccgggtgga ttttcctatg gcgactatct gcgctgcgga gcgctggcac
    61141 gcttctcgcc catcgtggag gaagtgatca gcttcgccaa acggggcggc ctggtgatag
    61201 gtatctgcaa cggctttcag gtgctcaccg aaagcggcct cttacccggt gccctgatga
    61261 tgaactccgg tttggatttc atctgccagc atcagctttt gagggtggaa agctcttcca
    61321 gcccctggac gcacggcatt ccgacaggaa cagttttgga catccccatt gcccacaaag
    61381 agggcaatta ctttatcgac gcggacggcc taaaagccct ggaagatgct gacagagtgc
    61441 tgtttcgcta ttgcggaccc gatggcagca tcacggctgc tgccaatccc aacggttctt
    61501 tgaacaacat tgccggcatc tgcagcccca accgcagaat tatgggcatg atgccccatc
    61561 ccgaaagggc tgccagccca gaagtcacat ctcaggatgg agggaagatc tttgaggcgc
    61621 ttgaacgcta tcttgactcc cttggctgat ctgctcttaa cgcccttatg ctttggctgc
    61681 ggatcaaggc tgcgtgaggc ctcagacctg atctgcgagg attgcctggc ccagcttgcc
    61741 ctcccaggag aggacacctg taagctctgc ggcacccaat tggagcaggg ggcctgtcct
    61801 cactgcgcgg agtcgattcc gctattccgg gtggccagat cggcttttct gtttaaaagc
    61861 cctgtcagag agctggtcca cgccttcaaa tatgactcct acacccgcgt gagcccctgg
    61921 ctgatcaaag gaatgttgca aacctatcaa tccgaacctg ccttttcctg ctgcgatctg
    61981 gtgacggccg ttcccctgca ctgggtgcgc cagagagacc gcggcttcaa tcaatccgag
    62041 ctgctggccc ggcaactggc caaacacctt ggcctcgcat acaagcaact ggccaggcgc
    62101 tcacatcaca ctcccagcca gaccagattg agcaaagaag agcgccagca caatctgggc
    62161 aacgctttca aactcaaatc caaacaagac ctgagcggaa gccacatcct gctgatcgat
    62221 gacgtcttca ccaccggcac cacggtcaac cagctttcca ggctgtttct ggatcatcag
    62281 gccgcttcgg tggctgtatt gaccgccgcg agagcgatat gaactacaaa aagcacccct
    62341 acgacgatgt ccacactcag atcgacccgg ctgaagcagc cgaaatgatc gaaaaggtgg
    62401 cccgcttcat cgctgaacgt cacttcgccg cggcggggat catgctggtg gaatcgctgc
    62461 gacccctgca cagcatcgga agccaggccc tcttttttat cctgcccttt gccgaaatcc
    62521 tctttgattc acgcaagtat cagcagttcg ccctcatgat ccagaacgag gacaacctca
    62581 aaaaactggt agcccgcatg gacgaactgg atgaagagat caaccgcgag cgcaggatcg
    62641 ccgcaaagct gaaacgcaaa cgccgccggg ccaaaacccg ccaatttata cataagatat
    62701 tcaaaatcaa ggataataaa gctgaataag cggaggaata atgcaatacg atgagaaacg
    62761 ccttacccgc atcgtggcta ccgactgtgg aagcacgacc accaaggcaa ttttgatcga
    62821 atgggtggat ggcgaatatc gccagaccat ccgtggcgaa gctcccacca ctgtggaagc
    62881 cccgctcaac gacgtgacca aaggcgtgat caacgccact caggaactcg aggaactggc
    62941 ccgtctgaag tataagaacg acaacatcaa attcatggaa aacggcgaat ttgtcatccc
    63001 ccgcaaaggc gacgtcggtg tggacgctta tgtctccact tcttcggctg gcggcggatt
    63061 gcagatgatg gtcaccggag tggtggcatc tatgaccggt gagagcgcag aacgtgccgc
    63121 tctgggagcc ggagctatcg tgatggacct cattgccagc aacgacaaac gtatgaacca
    63181 cgaaaagatc gagcgcatcc gtcagctgcg tccggacatg atcctcatgg ccggcggcga
    63241 ggatggcggc accatcaaac acgtggtgga aatggcagaa ttggtgagcg gtgccgatcc
    63301 caaaccccgt ctgggctcgg gatacaagct ccccgtcatc tatgccggca acaaagaagc
    63361 ccaaaaagac atccaggacg ccctcaaaga caaggtcgat ctgatcatga cagacaacat
    63421 ccgccccaaa cttgagacgg aaaacctcgg ccccgctcgt gacaaaatcc atgacctctt
    63481 tatggaacac gtgatgaaac aggctcccgg atacaacaaa ctgatgtcct ggaccaaggg
    63541 tcccgatcat gaagaagtgc cgatcatgcc tactccggca gcggtgggaa acatcatgca
    63601 gaccatcgcc aaagacgagc agatcgaagt cgtgggcgtg gatatcggtg gggccacgac
    63661 agacgtattc tccgtgttta cagaggaata tgtcttcaac cgcactgtga gcgccaacct
    63721 cggcatgagc tacagcatct ccaacgtgct ggcttcagcc ggtctggaca acatcatgcg
    63781 ctgggtgccc ttcgacatca acgagagcga actgcgtaac atgatcaaaa acaagatgat
    63841 ccgccccact accatccctt ccctgctgga agaactggtg ctggaacaag ccatcgccaa
    63901 agaagccctg cgcctggcct ttgaacagca caagcagttt gccagcagcc tcaaaggcat
    63961 gcagagacag cgtgacatct ctgaggcttt cagtcagtcc acttccgggg ccacgatcgt
    64021 caacctcatg accctcaacc ttttagtggg cagcggtgga gtgctttcac acgctccccg
    64081 gcgcaatcaa accgtgatga tgctcatcga cgctttcctg cctgagggga tcacccgtct
    64141 ggccgtggac agcatcttta tgatgcccca cctgggagtg ctttccgaga tctgcagcaa
    64201 agcggctacc gaagtcttcc gcaaagactg catggtctat ctgggcacct gcgtagcccc
    64261 cgtgggcaaa ggcaaactgg gcaaaccctc gctctatgcc aagcttgagc ttcccggcgg
    64321 caaggtcttt gaagaagaca tcccctacgg cgaagtgcgt ctcatccctc tgggagtggg
    64381 tgaagtggcc aaagccattc tcaagacctc aggcggcctc atcctcgata aagaaaagaa
    64441 caaagaactg cagaccgagt tccacggagg agtggtcggc atcgtgctgg acacccgtgg
    64501 ccgtcaaccc tttggactgc ccaccgaaaa aacgcaacgc attgaatacc tgaaaaaatg
    64561 gatgcgcgaa ctcgacgcct atcctcagga aatactggtt taggaggata aaatgggaca
    64621 agcatattct gccggactca ccgtaaccga tagcatcatc ctcagaaagg aacgcctcct
    64681 gcccctcaaa ggcacggtcc tggtcaaaaa gggcgacaag gtcaaggccg aggacgtcgt
    64741 ggccgaaact ctcctgcctg gcaaggtagt acccttcaac ctggccaata aactgggagt
    64801 gaccccatcc caactcaaca acttcatcaa aatccagccc ggacagcaga tcaccaaaaa
    64861 caccatcctg gctgaaaata aaggcctctt tggcctcggc atcttcaaga gcgaagtgcg
    64921 ctcacccgtc gaaggtgagg tggaatccat ctcttccgta acgggccaga ttctcctgcg
    64981 cgaaccacgc atccccgtgc aggtcaaagc cttcctggat ggcatcgtca ctgaagtaat
    65041 ccccaatgaa gggatcatca tcgaaaataa aagcgcctat attcagggta tttttggcgt
    65101 gggtgacgag acggtcggcc tgatcaaaat gctggctgaa actccggatg acgagctgga
    65161 gataggcaag atcgatgaat cctgccgcga caaagtgatc gtagccggag ctttcgtgag
    65221 ctatcatgtg atcgacgcgg cccgcaaaca tggcgtcaaa gccatcatca ccggcggcat
    65281 cgacgaccag gacatcaaga agctgctggg ctacgacatc ggcgtggcca tcaccggcca
    65341 tgaaaagatc ggcctcacca tcgtctgcac cgagggcttt ggcaagatca ccatggccca
    65401 gaaaaccttc gatctgctca agcagttcga cggccacaag acctccattc atggccgcac
    65461 ccaaatccgc gccggcgtca tccgtcccga gatcattatc cctcagcagt ttgacgagaa
    65521 tgaactgata gttcaggaag ccagcctgcc catcctggaa ccgggcaccc tgatccgcgt
    65581 gatccgacag ccccactttg gccgcattgc caaagtcacc aggctgccgg aagaactcac
    65641 caaagtggaa tccgagaccc tggtgcgcat cctggaagcc gaatttgaag acggcaccaa
    65701 gttcagcgct ccccgtgcca acgtggaagt catcgattca taaagagata caatcgcata
    65761 ccagcccccg gatgtatttc gttcgggggc tttttttccc cttttggggt ggcatctgtg
    65821 gggagccgga aacgtcctcg ttaccgcatc ccgtagtgtc ggatgccaat ccggcgagtg
    65881 catcagatat ttgatagaac gcagaagaag ggaaaaaagg gatgatcggg actctatcat
    65941 ctgttgaatc tgtgtgaacc ctctcaatat ctgcgaaatc tgcgcataaa tctgcgaaat
    66001 ctgcgggaaa cactctcgtt tctgggggat ggatagtttt ccgcagaggt gagcaatgat
    66061 tgtatagatt tcagaccatg ccacaagcgc tgaaagcgcc ttagcaaacg aggacgtttg
    66121 ctgttccatg agatcgtcac atagcataca ttgggagact ttcgtctttc acaagctttc
    66181 cttgcctggt cacccgccgt tgagatgccg tgcggccggg ggcgattttc ggcggagagc
    66241 gcccggcatc tccacggcgg gtcattgggc aaggactcat ggagctcttc acctggtttg
    66301 atatccctcc ctggatggcc gcggcaggtc tgccccagac tacctcccac ccatctccca
    66361 tttatggggc ttgggtagga gatgctggac gcgatcccgc gagcaacaat caagggcgga
    66421 aaaagacggc caaataaaag ccctgaccga agccagggcg ttgttatcag attgttgcta
    66481 agaggagtta cttggacagc accatcttgc gggaggagct gtattttcca gttaccatgc
    66541 ggtagatata gaccccgctg gtgacgttgt tgccctgggc atccttgcca tcccagacct
    66601 gctgataata accgggatgc tggtgatcgt gctcgaacct gcgaatcagc tggcccttga
    66661 ggttgtagat ctccaggcgc actttgcccg cttccttgag actgtagcgc aggctggtgc
    66721 tgggattgaa gggattgggg aaggccgtct ggagctgagt gacggatggg atttccggag
    66781 ctccgggatc gttgggctga ccgctgacgg tgatggtgag cgggccaaag agggttgaga
    66841 gaccacccag gtccacgctt tccagccaat agtaataggt gctcccggca tccacttccg
    66901 cgtccgtgaa gtcataactg acctgggatc ccagcccggt accctcatcg atgatgctgg
    66961 cattgagcct ctgggccagg gacacggagt cgttgctacc acgcaggatg ttatatccca
    67021 gatggttggt ttcagattcc gtcatccagg caatccgcac aaacatatcc gcggtgagga
    67081 cagccgtgaa gctgctgagc tcgacgggga gggtctggtt gtcgttgaca actacggtta
    67141 catcaccctt ggcatcgaag tttacaccgg caaaggtgta atcaggagtg gtggagggaa
    67201 tgggattggg gccagccagc agctgggtac cagccataat ccagcaatac cagtcggtgt
    67261 tccagtccat gggcttgagc accaggacgt cccacactcc ggtggcactg atggtgtaga
    67321 tgagggctgg gaggccgctg tcttctccca aaagagctgg aggaactggt ccgccggggg
    67381 tgaattccac tccattggga ggcatgcctg ggttgcctgg atcgatggga atgactccct
    67441 gagaggcctc ttcgatggag acgtcgtcga taaagatgtt gttgccccag tcgcttacgc
    67501 cttcaaagaa gatgtatccc acgcctttca tcaggatctc gggaattggg aagctatact
    67561 tataccaacc ggcttcatcc accactggtt caaggtccag gtgacggttt acagtcccca
    67621 accaggtagc ggcattgata ttggtgatag caccggcata gacgttaagc tggtctgatt
    67681 cagaggaata tcccgagtcg cggtacatcc agaagctaac ctggtaatcg gcatccccaa
    67741 aactgagagg aggcgtcacc aacatgcctg aagttccgtc gttgtagttg tagcaattga
    67801 attccgccat cgcttcaccg ctgtggggca ggcaatctgg attggttccc accagagtcc
    67861 tttcccaaag cccttcgcct ggtccggaaa tctgctggtt gatccatccg gccggcgcga
    67921 aagtgctgcc gtcaaagctc tctgcatagg ggaagctgct gacgctggga tcataaccct
    67981 cgccatccag ttggataata tgggtggatt ttgcaccaga acgctgggag gctttggttt
    68041 cagcgatgtt gtcgataatt tggaggttgg ctgtcattat gccttcagat tcaggaccaa
    68101 agttgaccgt aatctgagct gtggcgttgc ggaccagcga gatatccgcg gccagggctt
    68161 ccaggctgaa ttgatcagcg tgggtgcctg tgatcgtgac atcgtccatg tctataaaga
    68221 gagtcccgcc gccggtgttg gtgatggtaa acacctttgc aaccgagttt ccaggcatca
    68281 cttgtccata atcccaggct gtgggattga tctggaaaac cggatctgtt gccagcggtg
    68341 tggcagtcag atcgaggctg aaagtgaagg attgaggggc aggccaggtg gaaagaatca
    68401 ggtagtatgt tcctgctgca agctcaagat tggtgagggt agcaacggtt ccgccatttg
    68461 ttgctgagcc aagcaaagga gccggcgtag tgggattggg aatggcctgt gtgaaaagca
    68521 tcccaatgta gcttccagtt acagctgtca aagtgccact caaagagctg ttaacagcca
    68581 gggtaaacct cagcaccatg tcgtctccac ctagtgtcat gtcaagcttg acaagacgta
    68641 aagggtagaa atagttgtct cacaaagtga ggtgattatg attaagtaca agattacgtt
    68701 atcaaaagat gaaagagcag agcttgaagc ccttgttgac aaagggattc atcatgtatc
    68761 cagatttaag gatgctttga ttttgttgaa ctgtgatgag ggtataaatg cgagaaagta
    68821 tcctaatcgc gtgataagtg atgtggctaa agtaagcatg aagacgatag acagagttaa
    68881 aaagaagttt gtggaagagg gctttgatgc cgtcatgtac cctggtaagt caaagcgtgt
    68941 atatgtgaag aagtttgatg gggaactgga ggcacattta gtggctttat gttgcagtga
    69001 ggctccctct ggatatgcca agtggtcatt aaggttattg gcagataaga tggttgaatt
    69061 acaatatgtt gatgagatct ctcatgaaag cgtccgtcag attctaaaaa aaacgaactt
    69121 aagccttgga aaagcatagg atgggtgatt ccacccaagc aaagcagcga gtttgtagca
    69181 agaatggaag ttgttctgga tgtctataaa agaccttaca ataaggataa ccctgtgatt
    69241 tgcatggatg aaactccgaa gcaactaatt ggtgagacca gaattcccat tccacacagt
    69301 ccaggtacac cggcaaagca tgattatgaa tacaagcgta atggagtctg caatatattc
    69361 atggcctcgg agccattgaa tggcaagagg tatgttcaag taactgaata caagaaaaag
    69421 gatgactggg ctatattcat gaaatcaatt gcagataagt acaaagatgc cagaaaaata
    69481 accttggtca tggataatta tgcaacccac acaatgggct cattctatga agtttttcca
    69541 cctgaggtag cgaaggctct gattgataga ttcgagttta tatatacccc caaacatgga
    69601 agttggctga acatggcaga gattgagctc aatgtgatgc aaaaacaatg cttaaataga
    69661 agaattccag atattgcgat actgcagaga gaagtaaatg cttggacacg tgaaagaaac
    69721 agtaagaaat gtcagataaa ttggcagttt accactcagg attcaagaac aaaactgaag
    69781 cgtctctat
//
LOCUS       DAOSFN010000002        54676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_445_length_54676_cov_8.810384, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000002 DAOSFN010000000
VERSION     DAOSFN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 54676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54676
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_445_length_54676_cov_8.810384"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..1986
                     /locus_tag="PLO35_00300"
     CDS             382..1986
                     /locus_tag="PLO35_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78129.1"
                     /translation="MNKIYVTMALVTILSLLTIALPAQNHMTLAMSIFGDQDYDHFGD
                     KLASLDFNGDGYDDLAVLQSWWVPDSIYVTIPYSVSAGRNWGRILFYYGGPGFDGVAD
                     FTIEASRQYQFHFSKSYMFSLGDVNGDGYDDLGILEHFPKRLDIFFGGTNPSSSPGYM
                     IDLQDTSIYQAQFYKLGDINGDGYDDFWLSTYPYSTYTGNVSIILGGTFQQIIFDSTS
                     EADYSGFHAIGDINHDGYDDICYTIFRQRPTRFHSKTVYYGSPDLNLQNPVVIVPDYT
                     EAAAVVAKAVGDVNGDGYDDFIGLMTGYGQRLWLGGGTIGQDWDVLLNPPYGGSNSDH
                     CTVSGDFNGDGFSDIIGASFNLECAALWLGGSNMNGTPDLFLYGISGGMQYGVSMASG
                     DFNADGFDDVFIGEPSLSNDIFHGRIRYYTGNGQLEDTTVAVDDAINPPSVSNWGFRV
                     IPNPSKQGTDLRIRFYGEGFDRLLHAELRVYNLRGQLEYTQQISNAALKSGDVAIDKL
                     PLPRGVYLVAIYEEGQRLKTQKVTIK"
     gene            1996..3273
                     /locus_tag="PLO35_00305"
     CDS             1996..3273
                     /locus_tag="PLO35_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78130.1"
                     /translation="MRTYIHILLFICILAVPCSLPALYSDDFLIGAYSHIRSYSTSAA
                     CTDAIATRAREAGFNALTSYTAWETNDTAPSDMFGILDSDSLDVIYEDLIFNPDPDPS
                     LEKYGYAALSSGIDMKFESEYADASEVNSEDSFLDTFFYMSSSAARVGSFGYDEDYSN
                     DNYWSVPDSSSGYAYKDITYRWPHGAHYDRVGNEFRFLGYTTNGIDTPENNKLTVRYV
                     FRVDDLGSYSDSDTLAVFSFVKYDGMTTEFTVHDSLYFYQGSAFTKKYVLTKAIYESL
                     PLSDTSSLTGCKALEFQFPLKGSFAHTLDSQGMLSDNAWHLQMNKSLNPWMYWCGRGS
                     LRLDYIEYYDDVFANYTNNPAKRAKLRNFESVANLRHFYGMDEPKAPNFESFKKLREH
                     LQATFPMDARYIVTANDLDRWHLEKPDTTRYRR"
     gene            3218..4738
                     /locus_tag="PLO35_00310"
     CDS             3218..4738
                     /locus_tag="PLO35_00310"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78131.1"
                     /translation="MTWIAGIWKNLTQPATGVSLLQNMNGTDIQQCSFANTINGKQGT
                     GIHLTAASPTISTCNFTNLYRGILTEFSLTSGVALESSVTESNFNNCEMGLESRSSYH
                     RLKANYFNRNNSSIVNHTGSNLNLSYDANNVLMNHNANIVFYDTMPCESTIQLFTGHN
                     DFYHLTDDSTSLDAIDFSFDANYFDFPLTLDFSINASKNWFQDDQVTVNNPAYVDFVY
                     VDLYDPSSSMPAPSPENDRLFIALEYESQELYELACATYQAIIDDQLEEEQTYVTSAI
                     DGLYRSTYMIPDPAWELTDYFDTKALQYAIDDPALSAILNDYLAKVFVLDKDFQAAVD
                     LIQLRIDTPISEIDSLRAVLDLEIVLQLAAMEEDKRPLTTKYAQYQYPDIQVFDVMHS
                     HNWNKYNRALHQNDQETNSVIAPTPQIQSNYPNPFNPSTTIAFSIPETGRVRVSVYNI
                     KGQKVKDLLNTELTRGNHRLVWDGKDANSRNVASGIYFIKLESGGKTSIRKAMLMK"
     gene            5282..7297
                     /locus_tag="PLO35_00315"
     CDS             5282..7297
                     /locus_tag="PLO35_00315"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOH78132.1"
                     /translation="MNRIQPLVWLLLFAMVLPFVTLGAHAVLPADTMDHDLRFTETNP
                     PAAPVRPIAEFEPASHVLIRYPLGIPVALVAHLSNTAQVICLVSSTSQQNTATNTFNN
                     GGVNMSNVSFMIAPTDSYWTRDFGPWFIMDGNDQVAIVDFVYNRPRPNDNMIPQTFAT
                     QQNFTYYGMNVQQTGGNYMSDGINTAAQTTLVYEENTSLGQTGVSNKMLQYMGASNYY
                     VINDPNNTYIDHIDCWAKFLAPDKVLVRSVPTSHAQYAAIEQAANYFATRNCAWGYPW
                     KVYRVNTPQNQPYTNSLILNHKVFVPIMNSTYDAAALQVYRNALPGYEVIGISSPSST
                     PWESTDALHCRTHEIADKQMLHITHMPVWGQHNESETINFNARIKAYSGQALYSDSLK
                     VMWKVNQAPWESTLLQDLGSNNFTAMLAGFAAGDTVRYYIHAADASGRSIDHPLTGAH
                     DPHIFSFYPDQVPPVIQHTPPVSVINQDSGPITFSAQVSDNVWVQQVLFSYYSETQPL
                     LSIPMTDADGIYTFEYYPEFTEDDHFLWYQITATDTANPPNQAVYPAPGEWQSIPLMI
                     VDNDDPVIPEVDLVIQSVYPNPFRPTRESASVAFKGKAGEKVSWSIFNIRGQKVSAGL
                     STVNSDGDGRISWNGKGPDGSFLPAGIYLLRLSQSGSHQSRKLIIGL"
     gene            7485..9989
                     /locus_tag="PLO35_00320"
     CDS             7485..9989
                     /locus_tag="PLO35_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOH78133.1"
                     /translation="MRTLSFPVVLMIALIILPFNLSALRSVARLDNPPPGMAEDCDAL
                     GYDVASYHPGVYLDLVVDEAELAALKLQNGSWYVTQTEAQLREALTARRDIPGYRDYD
                     QMVSELMQLQAIYPNLVQVSTIGSGWGSIYSQTVPYYTGFDHDLWAVKVSANVSQEED
                     EPAFYFVGEHHAREPISTEVCMEILIHLVENYGSNPEVTSFLDNSQVWIVPLLNPDGH
                     KIVIDQTDVWWRKNIRDNNNNQEFNTDDSSGMGPDGVDINRNYGYRWGYLSATDNMQM
                     PTYHGPYAFSEPEASAFADFISSRRFLAGVSYHTYGQYVLYPYGFVGDINAPDKVELQ
                     ALATDMANVILKQGSTTQHYDAMPSWQLYPVSGSSDDWIYGTTGSFAYTIEMATQFIP
                     NQNQMQQIITQNLNGAMMLLRRSQKKMLTGHVTNAETGAPIPARVWIDGYDNHPLGFA
                     PICADSTFGRYYRLLPPGQYEVRYFLPGFYPQTRTVVVTADAVSVEDVQLTPSQPMEL
                     FIQVRGDFFGGVSEAQLVFDQDPEHVYTTDSAGWIIIPEFYPGEYNVTVSKPGYSTLR
                     VTRMIETTSIGFKLSSMAGFDDGFENGMGNWNTNGSWGVSSAQVYSGASALADSPAGN
                     YGSDISKWCRLSQPVNLTAVENADLQFHISHNLSNDGDYCLLQYSLDGQNWLVFDSFT
                     GVAAWTLKSYSLNSFIGQSVHLRFLLVTNSSANADGVFIDNFRVYTGSLLTASDDLLA
                     SAVKLQSWPNPFQDKLSLMVSGLRGGEADLSLSVYNLRGQKVWQRTGLSAKDGYLDLE
                     WNGLDSQGRAVASGIYAVKLTAGGKTLASRKIMRVK"
     gene            10037..11230
                     /locus_tag="PLO35_00325"
     CDS             10037..11230
                     /locus_tag="PLO35_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH78134.1"
                     /translation="MEEIRIQNISKEFDGFLAVDSVDLTIKSGELFSFLGPSGCGKTT
                     LLRMIAGFEIPLRGKIYIGDQDIGDLPPYKRPVNTIFQNYSLFPHMTVWDNVAFGLKV
                     KKVALPEIKTRVSEMLELVKMSDQSSKYPSQISGGQKQRVAIARALINKPKVLLLDEP
                     LAALDLKLRQHMLIELMNIHDAVGITFIYVTHDQSEAMSISDRVAVMDQGRVIQSGPP
                     DDIYERPASIFSAYFIGETNLITGEVTGIEDEYVQLKCPMGDDQHFEIDSTFHFRPEL
                     GQSLTVSLRPEKIAISRSKPRYQDDINMLRGTIEDILYLGSHTQYIVKVGKLKFRVFS
                     QHKRVYFDDKALDWDEQVWLFWHDTDTWLINEMPSDESRSPEGTSHLQRWAKRDLNRG
                     AQGDQ"
     gene            11279..12115
                     /locus_tag="PLO35_00330"
     CDS             11279..12115
                     /locus_tag="PLO35_00330"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH78135.1"
                     /translation="MDYLLAAPTLLWLTIFFLVPYFIVFIYGFLTPDIFDVKFEFSLD
                     AYRQIFNGTYFKPFFLSFRLALLTTMLCVLLGYPVAYFIARAGDKLKNTLLVLIIIPF
                     WTNLIIRIFSWRIFLSYNGVLNDFLISTGLISQPLEIMRTDLAVILVMVYVYLPYMVL
                     PLFSVLEKLDFTLLHAAMDLGANEVKAFFRVTLPLSVGGIFAGVLLVFIPALGAYLIP
                     QLVGDQRSLYVGQVITYKIKNIPRNWPTASALSFTLLLLVAVCLLIGYSMYRNRQKAR
                     RF"
     gene            12115..12915
                     /locus_tag="PLO35_00335"
     CDS             12115..12915
                     /locus_tag="PLO35_00335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH78136.1"
                     /translation="MKAFRLSSLFKGLNLTVFTLVMVFLYIPIVILVIFSFNDARIVT
                     SWKGFTLKYYAQLWENVAIRQAFWNTMLIAGFSTVISTLLGLLTALGLENKKLRGRRG
                     FMALLYAPLVIPDILMGVSLALLFNFLRVNTGMMTVLIAHITFCVSYTVIVIQSRLED
                     FDYSLVEAAMDLGAKPNTTFFKVKLPLIMPGIIAAALLAFTLSIDDFIITFFTSGRGF
                     DTLPIYVEGTIRRGTITTINSLSTLMIGFTLFLVVFTKRIRKSIISFF"
     gene            13048..14253
                     /gene="pgsB"
                     /locus_tag="PLO35_00340"
     CDS             13048..14253
                     /gene="pgsB"
                     /locus_tag="PLO35_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HOH78137.1"
                     /translation="MSVLILATVLLVLNWIWEYHRHLRNVKSIPIRIHVNGTRGKSSV
                     TRLIAAGLRAGGIKTLAKITGTLPRVVLPDGREAAIIRLMGANIIEQKYIFREAIAHK
                     PQAVVIECMAVNPVFQWITERKFVHSTISVITNCRPDHLDLMGSTEQSVTMSLSNTIP
                     VNGTCYTAENEHYKLLEHVAKRRHCKIHQIRPRDVTHEELAGFRYIEHAENVQLALAV
                     CAEAGVPREVALEGMRHAHPDPGALKKYIVEDRDKTIHFYNVFAANDPKSTARVIDMV
                     TGHLKGVEKIIILNSRSDRLFRSEQLVDAVRELDFSYLLLTGEIPDKVESYALRTGIP
                     KNKVFPLGEPLPEVVYQKAWELTGKESHVLGIGNIAGRIKYGAQIVAHFKHKIQAGKK
                     ARKQGAVSG"
     gene            14246..14701
                     /gene="pgsC"
                     /locus_tag="PLO35_00345"
     CDS             14246..14701
                     /gene="pgsC"
                     /locus_tag="PLO35_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOH78138.1"
                     /translation="MVDAVVQAAVGIGVIISLIFSELLGASAGGIVVPGYVALYLDKP
                     MQILGTLAVSLLTWGIIRIIGKFTLLFGKRRMVLSILIGFILGWATRMLVFENQTVYT
                     LQMQSIGYIVPGLIANWFERQGFWKTISTMGVAAVLVRLTLMVVFGGEV"
     gene            14698..15825
                     /gene="pgsW"
                     /locus_tag="PLO35_00350"
     CDS             14698..15825
                     /gene="pgsW"
                     /locus_tag="PLO35_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOH78139.1"
                     /translation="MSMYRPSLKSGWSLILLFVFAVGLYVLAQSSYVHIKTDNYEEKV
                     QAARLMQTYMDSLKAELKATGYEFDPIDDPFHTGLIGTRLSSITTDRGLLSDKQAALN
                     PNLAAIFVEEFKKERLSEGDHIAVGISGSNPAVNLALYAAISVLKLNPAVITAVSSAS
                     YGANREDYTWLDIEAVLKKKQLIDFSSSYASLGGKDDLAIGLSDNGLASLRDAMTRNG
                     VPLLLGSTLEENVSLREAAYKELLPEGQRYRMFVNIGGGLANVGSEPNSRLIPEGINH
                     KLAEKEFEKQGIMMVMARQNVPVLHVRRIQRWARKYEVASTQEKIPIPGEGPAFSSRK
                     HNVSIASICLALLVAAIVVVIVFDRHDRRFMANIVDPDEEL"
     gene            15900..16724
                     /locus_tag="PLO35_00355"
     CDS             15900..16724
                     /locus_tag="PLO35_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78140.1"
                     /translation="MHFKQILPIVAIAAMSFSALQAKPKALNFDNPGNKRLLKTEAGN
                     YYYYRSLPEKSMKLNVAGIDKLELRSFAIEPLKKPQVISIVAKKQTVHDLSLKSRLDG
                     YYLYESVTIPIPEGIKELEILCYERSIYFRAFWTVPPKPKPAPKQKLPNLSIDAHSGI
                     LAMTHNGSSSDYHSFTPDQPLKFTLNNGRDAVLYVRARLLDRTLPIFEVYVNGTLVQT
                     HEFTLKRSTKYSVTGIRHLSIGMKVELPANQGKSVIELKAKSDHLFLGRPVLLKAN"
     gene            16733..17881
                     /locus_tag="PLO35_00360"
     CDS             16733..17881
                     /locus_tag="PLO35_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78141.1"
                     /translation="MLQKHHHSPERDKHPDAALEEKDVLIQDGEQEETKQDVKPGSVR
                     TSRNFLPIIMSWCLVFLCAWLPLSAQIEGDISLSTQYSDNVFQLSEYDLDRFDQEHPN
                     LGYVDTTDDLSLKAQVDLSYPFRHKWYRLVPSITASFDQNVSNTDKWRRDIIARFAVI
                     RYYWDFKVMYGFYPNILVRAYVDEDGTGELEHYGYQRNLYRADLSVKPLEKTTLRAHF
                     RYEELLYNQFFTEFDGDAKTGGIGIRQNFPVFVLEAGYQYRVFDNWENGPLQDDSSYE
                     SNIYTASLRLKDMPLNDGKSSSPRWHPSLSLSFEERFFQGLDDWYGGRVDKIYTTSAG
                     LSFRLNKKWNISLDYSHYLRNVDSPNASVRRLKPYSENRLGAEVKYSL"
     gene            17894..18223
                     /locus_tag="PLO35_00365"
     CDS             17894..18223
                     /locus_tag="PLO35_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78142.1"
                     /translation="MVFKQESSTKKIKEFLDLVENHKIELRHFKSPVLVWAVQDGTVH
                     YSFWEASLLDQYGLDNVDGWDYEEDALFCPDWVMDEEDEDLDDDDDDDDLGLICDIKI
                     PRIKKED"
     gene            18230..19246
                     /locus_tag="PLO35_00370"
     CDS             18230..19246
                     /locus_tag="PLO35_00370"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOH78143.1"
                     /translation="MRVAVIGGGGWGLALSRLLAINGHEVLVWEHDSAWLQRLQSTHS
                     NPVLLPNIILPQSVNFTGSFAAIANFGSDVIILATPTQFIRATLRSINPEERGLWTSS
                     GLKAIVNVAKGIEESSLCRVDEIIAQELPASARHLICALSGPSHAEEVSREVPTTVVI
                     AGGDEDTLIMLQNLFSNSWFRVYRSDDLIGVEIGGAVKNVIAIAAGIVAGLGFGDNTM
                     GALLTRGIVEIGRLGKVLGAKPETFLGLSGIGDLITTATSPHSRNRFVGYEIGKGRRL
                     RDILADMKMVAEGVASTRSVRLLAQKHQVEMPITEQVYLTLFEDKSPALAIRDLMTRE
                     LKEE"
     assembly_gap    19599..19698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20186..20788
                     /locus_tag="PLO35_00375"
     CDS             20186..20788
                     /locus_tag="PLO35_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH78144.1"
                     /translation="MDASALKRERILSAAMSVFASRGYDKSSMEEIASRARIGKTTLY
                     YYFESKEAMFLDDVQAAYNSFFAVIEEKLAIHTRFEDRFRAILKLPIRFIFESMPILM
                     EAQNSIPPAYLEVLESLKAKGRDRMLDLLRDIMEAGRQEGLLDAKLSVEDTIFVVHDW
                     MLMTEMNLSPGDKDRILKRLERDQDNLIDMILYGIIKRGQ"
     gene            20788..22164
                     /locus_tag="PLO35_00380"
     CDS             20788..22164
                     /locus_tag="PLO35_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH78145.1"
                     /translation="MKHAMLAAGFCLLISLAHAIGLEESIEMAKRNNRQLLIAKEEVE
                     KARQTYNDVRGNLLPQLTMQGGYELSRTNLPPSAIPAPVSFSGGLSATASDDDQYLGA
                     ILDNVVNSMIPADPMDEASVALQLKMTQVLFLGGKLINGIKAVDRYRSIQRLNYTLEE
                     QKLVLNTTELFYQCLLAGKLASVQEEGLELANRHLARVESFFSEGMVSEFDLLRARLE
                     VAKLQPQVLEAQNNYQLVLAAFRRHLGSEDPELKPEGEFSLPPEWEISLEEALSAGQK
                     DRVELELADIATEVAKIKYNAEKGNYLPNVALQADYSLYTAADDFGIERDDFGSRYSV
                     GIGIQIPLFTGFSNTSKRKLAKSSHYQAVLQQQDYEDLIALEIKQDWQNWHHAKENYR
                     VQSENIKMAERSLELAQVRYDNQVGIQLEVFDARITLSGIRLQYLQAIYEVIVKQLKL
                     QKSIGTRI"
     gene            22175..23212
                     /locus_tag="PLO35_00385"
     CDS             22175..23212
                     /locus_tag="PLO35_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH78146.1"
                     /translation="MRRYIVFTLAVLIALSLVGCGGKKDKAKNMQQIQADQGIPVRVR
                     IMGTETYVQQLRYNASLAGSEESTAQAMVGDVVTQIHAKVGDRVSKGQVVISFPINTP
                     AAQYEQASTAFAAQRQAYERMQRLFEKGAVSRQDLDNLETGYKVSKANLEASEQMIKV
                     RAPISGIITAIHVNLAEKVYPGKDLFTVTATGGYKAVMMVPDTEVARIKKGAQVSATV
                     NGETINGRISQVAMALDPQSRAVRVEAVFPGVNKNISYGSNAQINVTVLSKPNCMVVN
                     REHIGFENGAAWVWVNENNRAVRREIQTGLSDQLHFEVITGLNDGDQLITEGNSLLSE
                     NALIRLIDQGE"
     gene            23216..26293
                     /locus_tag="PLO35_00390"
     CDS             23216..26293
                     /locus_tag="PLO35_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOH78147.1"
                     /translation="MFLSKLSIERPVLVTMAILVFVVFGMLAYIDMPLNLMPDVQLPF
                     VSIQVVYPGAGPEEVETQVTKKIEDAISTVSRIDYVQSYSLESVSIAIIAFELGKDVD
                     IANQEVKDKVDGILRDLPSTAEKPVVQKFDFSSFPFMDIVLSGSLDSRELYELADTKI
                     KDRFAQIEGVAQVSMTGGAKRQIDVKLSDSSIYENKISLAQLMQILAAQNMDMPAGNF
                     NRGSQEYSVRLKGEFGSLDEIGEARIPTGAGMKKLSQIAKIEDSSEEVRTRAIYFDVS
                     RKSLKDNIVRMSLIKSADGNVVDIAKQIAAELPKIQKELPQGTDLQIIRDDSEFTKST
                     VSDTLGNIWMGILLTGLILFLFLHDLRSTLIVALSMPISIISTFVFLQLMGFTLNVLT
                     LMGLSTAVGILVTNSVVVIENIFRHKDMGNSRRVAADIGTAEIAVAVMASTLTNIVVF
                     LPIASMSSMVGQFFKEFALTVTFATLVSLATSFTITPMLASMIIPEGKHDSRFGLRFD
                     AAFARFAGLYQKFLSAVLRSKKRSLGIILATLLMLIASFALMPVVGLELFPAVDKGSV
                     SIMVELPQGYNLQQTAKVFDEIHQRIGKYKEIEHIVTNLGTQGMIDTGVNLAAADLKL
                     VDAKERKRSSNQMVAVLTKELADIPNARIKVSMPESFGGRVAPIEFYLQGQDNNKLEE
                     LKLKYIKTLSDVPGLINLDTSTREGRPEITIVPNRDQMALAGATVYDLAMALRASVEG
                     FVSTQYSESGNQYDIKLSLEDEYIDAPDKLMNLTVPIYGQNYLLSQLARVDFDSGTNR
                     ITHRDRYKSIVFTGGLTEGANLGDVVSEVQERVDKIDLPSGYQVVWGGNAEMLNDTVV
                     DMARTFILAVLLTYMLLAAILESFAQPLLIMATVPLALIGVILSLFLTGQALNIFSMM
                     ASIMLVGIVVNNAILILDYVNQRRKDGLNTHDALLEAGEHKLKPIVMSTLSIVIGMLP
                     MALGIGSNMKEFRQSMGVVSIGGLIVSSFLTLIIIPAFYYLTTKQSQNKLSEE"
     gene            26395..26826
                     /locus_tag="PLO35_00395"
     CDS             26395..26826
                     /locus_tag="PLO35_00395"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HOH78148.1"
                     /translation="MLTQKLYNWNGTLGNKYINNFAFWILSWAQVYSAAVTIDYVLLN
                     TVEFWTGSNPLAMKNGEEKVQYTEVDGSKFKLRVTNDNISIEEIAGANVGKKVDVKYD
                     AATQSWYLAQDSGAVKIATMNGDKMNLLYPSGNHLTVDVSY"
     gene            26881..27111
                     /locus_tag="PLO35_00400"
     CDS             26881..27111
                     /locus_tag="PLO35_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOH78149.1"
                     /translation="MIDKEKLQAVLDKIRPAIQSDGGDVELVNVRDDDVVEVRLTGAC
                     KGCPMATLTLKAGIERIVKEEIPEVKEVVSVK"
     gene            27125..28888
                     /locus_tag="PLO35_00405"
     CDS             27125..28888
                     /locus_tag="PLO35_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78150.1"
                     /translation="MIYTVKGSVTLATSGIVMVLFTVFFLPLRAWDADLGVGIGLAHA
                     SYRTEGEFPDFASKVSYFRDFPTVFLEAGVPVSEILKLNLRIYPHSRNYSLVDYDDDG
                     PQLMEYYLDMPLSLRARIGSLEVGAGAGVGYKFTHIGISEIHSIDDIEECEDHLPTFM
                     PSFNVGARVPLGKGEPTSLNLDYYQDLGPFSEAWGTKVYHKRFLATLSYRFKSMSPRQ
                     DLLPTQKIAASLGDTNLAIGAGFHLASMKVERVPEETFESINHKRGIWLPALEIGRKV
                     HPWLELQTGISTNKRQFSIKEYDDYEDRFSLEANYVDIPLILRAKLYGVEMGAGPTLS
                     VLTAAKYKRRGDLEPEHAPYEAARLIPGASLGFRYAKGRMGLNLYYCKDLTPFSENYH
                     IDKYQDRYLCYLSYRLGQAEPLNDLLPRELARMQPSESHLFGGLKKSIWGGSGKYSVV
                     MGLDKVSRFPSGFGFGYRLSAAALRSKFDEGEALSMLDLAAQARFSYKKGIGELWLGP
                     GFGMGSGLAVTDAEYFPIFPFLVPKACVEAGVRVHVLRQVAVDLSWEQSSILFLMSGF
                     GLGSTTTSSFGVPYLGLALGF"
     assembly_gap    28977..29076
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29418..30272)
                     /locus_tag="PLO35_00410"
     CDS             complement(29418..30272)
                     /locus_tag="PLO35_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH78151.1"
                     /translation="MLVIAKVADYEITELEITSELVDPRAETEQMRRERIITLTRLID
                     SCLLYREAQSQGIEADEDEFDLALLQSLEALDDSGNGSPLSSGEAKALEDKITRKIVV
                     RKYIRQICGNALEIDETQLMAFYQDQIELFSDPEKVRASHILIGKGSANAETRARLIR
                     QKIGNEDDFNQACSECSDCPSNMRCGDLGYFRRGQMIPAIEEVAFSMRVGEISPVFSS
                     SFGYHILMLTDHKQAAPIPYDDIKDGLRARLVTLEKEFFLLKHLKDLREKYRDLIQIL
                     DANYNEAL"
     gene            complement(30295..31239)
                     /gene="miaA"
                     /locus_tag="PLO35_00415"
     CDS             complement(30295..31239)
                     /gene="miaA"
                     /locus_tag="PLO35_00415"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOH78152.1"
                     /translation="MIPLVTIQGPTASGKSALAQALAMAFGSGIISADSRQVYQGLDI
                     GTAKPTLEEQRRVPHHLIDIIDIKEVYDAGSFARDAAGPIREYASRGLIPIVCGGTGL
                     YVKALLEGICELPPIPGSIKLSLSARIAHAATPEARIKLLNEMHAELRAIDPEFAARV
                     SVHDPQRIIRGLEVWMATGIPLSEHWRRQQPSETYKTFNILIMPPREQLYKRINLRMA
                     QMLESGLIDEIKSLLEQGYTWSDPGLNSLGYKEFKPYLEGTCSLEDAINLAAQHSRNY
                     AKRQVTWYRKLDFDLTIASNELNMSVLESRIREVLSLI"
     gene            complement(31236..33203)
                     /gene="mutL"
                     /locus_tag="PLO35_00420"
     CDS             complement(31236..33203)
                     /gene="mutL"
                     /locus_tag="PLO35_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOH78153.1"
                     /translation="MNRIKVLSEDVRNKIAAGEVIERPSSVVKELVENALDAGATMIT
                     VAVEAGGRDLIQVSDDGHGMSRDDAMLAFERHATSKIRTVEDIIHIGSLGFRGEALPS
                     IASVSELSLITRQTADQTATRVDFSNGRLRNVDQTASNPGTNITVKGLFKALPARRKF
                     LKSAQTELRHILKYFHYQSILWPSISFRLISEGKTLLNYIGTRDRRQRMAEVFGSGFF
                     EDDVIPVQTEREGYGVEGYIFGLEERRDKLIDIQYTFVNGRYISDKTLRHAIRSAYEP
                     FILKTRVWAKGYTPPYMLFISVPPEQIDVNVHPAKLEVRFREQQKLHALVHGTLTDAL
                     TDYENAIFASARDKFHQAAAVSEASTLEQSIYRSRVEVPRYSSYKKEFGELWQDDLFA
                     PPPRKDVALDSDAANGKVSGSARDGDEVSEASGDAYGSPREVTEGSGDVSGSIKESTP
                     PPQYRILLKNEEDYINPWQLHNSYIFVQVEDGLVIIDQHAAHERVIYEKLIQRAHGAP
                     PIRQKLIVPLVVDIPPYIATDIRELVDQNMELLEKTGFVLKKFSGSSLVVEEIPAELS
                     DWQGGKIFIDILKQLESELEENQDFRDSLAKSIACKAAIKAGKPLTRREMLALINDLF
                     ACQTPFFCPHGRPLIIKMTMQEFDKKFKRLL"
     gene            complement(33237..34898)
                     /locus_tag="PLO35_00425"
     CDS             complement(33237..34898)
                     /locus_tag="PLO35_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOH78154.1"
                     /translation="MNIDIRLNGIQHSMIELENPKPIAAVIRETDINPHSVLSYKINH
                     TQYVNDEYVLQQNTMVNCITIRHPEGFRIYQDSAIFILGKALYNLLGDKHSLVIEHSV
                     GDGVFCEVFGTDKFTEQDCARIKQEMRVIVEADLPIDREEVKLSEALDIFMNMGRKDL
                     QKNLRYTYRDSVSIYRCGRYYDFYIRPLADRTGMIDEYDIVYQAPGFILRFPSGKDLT
                     IAKPFKLPQKIFALHQEHDKWLDILNVHNITDLNKLVDNYDISQFILVEEALHEKRIA
                     EIASSIVKKPKVKLILIAGPSSSGKTTFAKRLSVQLQASKKKPIVLGMDDYFIDRDRT
                     ARKENGDFDFESIYAMDLEFLNQQLTDILAGKEVELPMYDFTRGLRKRSNNFVKLGKD
                     DVLVMEGIHGLNDLLTASVPASSKAKIYVSALNQLNIDNHNRIPTTDCRLLRRIVRDH
                     QYRGYSAIETLQRWVDVREGEEKNIFPYQENADYMFNSSLTYELGILRKHAWKLLQGV
                     SKNSSAYMESRRLLHLLSHIRDIPDALIPYNSIIREFTNGSIFRY"
     gene            35007..35963
                     /locus_tag="PLO35_00430"
     CDS             35007..35963
                     /locus_tag="PLO35_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOH78155.1"
                     /translation="MTKLTKAQRLRREITHLAGIIFGAVFFAIGYSWFLLPYNIAPGG
                     VGGISQILSFYTGIPNGVGMILINLPLFVISFIFIGRSFGAKSLYGMLVTAAMTDLMS
                     YPMLHKLGLIADLAKYTHIVNGRTVYAMLGPEDMMLTAIAGSVMLGLGLGIIFRFRGS
                     TGGTDIPVALIKQKAGISIGSGYYIVETGIILAVAIFLRDPKILIWGYINLFITTKIT
                     DLASEGLPYIKGAYIISQEVDEIKEEIYKKLDRGVTFFKGLGGYDKKEIDVLFCVLNR
                     RQVPLLTDIVKDCDPDAFMILTDVYDVLGYGFKSRKIDLSDG"
     gene            complement(36132..37559)
                     /gene="gltX"
                     /locus_tag="PLO35_00435"
     CDS             complement(36132..37559)
                     /gene="gltX"
                     /locus_tag="PLO35_00435"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOH78156.1"
                     /translation="MEEIRVRFAPSPTGWLHIGGLRTALYDYLFARSLKGTCVLRIED
                     TDRSRFVEGAVENLIGSLRKLGLDYDEGPGLGGDYGPYTQSERLELYHREAQRLLESG
                     HAYYCFCSQETLEEMRANQQASKEFVKYDRRCLKLSRQEVETKLAAGEKHVLRLRMPD
                     ERKFGFDDLIRGHVEMDASQSDDQVLLKSDGFPTYHLAAVVDDHYMRISHVIRGEEWL
                     SSTPKHIWLYECLDWQAPQWVHLPLILNPDRSKLSKRMNDVSVESYLQKGYLKEALLN
                     FIALLGWHPADDRELYSLAELCEIFSLERVNKAGAVFDLTKLDWMNGQYLRDLPLETV
                     VERSLPFFVEQNLPILEKETLKRMVGAARERCSLLSDIPSYCRMFISPNALEGEDLQS
                     VRSDSAQLVLSWFATQIPAAMPLNPEKVEELVKRGIAETGLKGKAFYTPLRLALIGQA
                     HGPDLPSTFAILGDEEALKRIRSWL"
     gene            complement(37576..38193)
                     /locus_tag="PLO35_00440"
     CDS             complement(37576..38193)
                     /locus_tag="PLO35_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78157.1"
                     /translation="MLTRRIALNASDLNAIMDLEARLHPQGNLLDAYKLLCQSYYGPS
                     HMGKDLEVIQISLEQELAKNRKLYQPLLQDIGNRKGFYRISLDLIPFFPAWGEEPIPV
                     SSLPKKLIKQVPDDAQVARVAQMILDSCLEMEIDVRSWKATWRRLWPRIEARYKPSPE
                     LVKEVQAHVLEASIPHHSSPFKSYCQPHYRILHHKVLKQAFDIDR"
     gene            complement(38168..39652)
                     /locus_tag="PLO35_00445"
     CDS             complement(38168..39652)
                     /locus_tag="PLO35_00445"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH78158.1"
                     /translation="MIIHNARFYLDDSFANDVKALRVENGIIVELLSTLPAGLGPEDL
                     DLGGDYAYPGFIDTHTHSFSGGLYSGGIDLQDCQDLSSLINRIGGAARDLEPGALLFA
                     WNFDENSLAEKRFPTQEELDQAAGDSHLLLRRIDGHSCMMSSHSRALIGAIKSSDVIL
                     RGEDNDRATHWFHSNCDETGILKAYHSAAKVAMKGGFCTVHTMIGDADQSIDHFQFLQ
                     ARSSELPIEYILYPQSFNIKAAMAVGAKRIGGCILADGSIGSHTAALSTSYEDSDTRG
                     SLYHDDRFWREFITSAHQHGMQVCVHCIGDAAIRQINTIYLELQQKHPADLRHQLIHC
                     ELTPDDLVAQIKASGAVPVMQPVFDLLWGGENGFYALRLGTARAAQMNRFASFTAQGV
                     KVTGSSDWYVTPMNVAMSVYACIHHRNPSERLTPVEAIRIYTANAAWLSHDEHRLGKL
                     SPGFQADLSVLDTDLTAAFHYRDVQVKYIVKKGKSVHAYSPHSS"
     gene            complement(39649..40230)
                     /locus_tag="PLO35_00450"
     CDS             complement(39649..40230)
                     /locus_tag="PLO35_00450"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOH78159.1"
                     /translation="MIHKLLENTKVVLASASPRRRDLMKMMGVKALVVPSHADEPDTG
                     EAPRVQALRHARNKARAVAARVDSHCLVVGADTLVVLDRHLLGKPDSIDRASQYLRLL
                     SGRSHQVYTGICLVYQGRCLQAWEKSSVTFKALSEEEIRLYVETQEPMDKAGAYGIQG
                     YGAQFITGIKGCYFNVMGFPIHRFYQLCQELLA"
     gene            40372..41457
                     /gene="serC"
                     /locus_tag="PLO35_00455"
     CDS             40372..41457
                     /gene="serC"
                     /locus_tag="PLO35_00455"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOH78160.1"
                     /translation="MERVHNFNAGPAVLPEEVMLEAQADFFNYKGMGLSVMEMSHRSK
                     EYQAIIDETYAAVKRIYGLGDDYEVLFLQGGASMQFLMVPLNFAFDGKEANFINTGVW
                     SKKAIGEAKKIGKTVHVAATSEDRNFSYIPKSWQLSDNPSYLHITTNNTIFGTEWKTD
                     PQVPAGIPLIADMSSNFMSKPIDAQRYSMIYAGAQKNVGPSGTAVVIIKKELLEKAIP
                     GQPSMLDYQLHAKNGSMYNTPATFTIYMIGLVLKWIENFGGLAMIEQNNIAKAKYIYD
                     AIDASNGFYKGTVDVADRSLMNITFRMPTEELEAQFISEAKKNGMIGLKGHRDVGGCR
                     ASTYNSLPLPAAHALGEFMRAFQQQHG"
     gene            41577..42194
                     /locus_tag="PLO35_00460"
     CDS             41577..42194
                     /locus_tag="PLO35_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOH78161.1"
                     /translation="MTVFSLFLRGGILMYVLLLLSVAVIAIVIEKYRLLRSVRNSNRK
                     IQSSLHQQDKLDNVRAILRVYGSNSPLGAMLNKLYNSNSKDIALIKESMESTANHELH
                     KLERGMGWLSTFAAIAPLIGFLGTVIGMVRVFMNIQGQSQNGIDINILAGGIWEALLT
                     TIGGLVVGIVAIILYNDLVVNLENIAKEMQEQAIEYVIRYQKTEE"
     gene            42198..42611
                     /locus_tag="PLO35_00465"
     CDS             42198..42611
                     /locus_tag="PLO35_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOH78162.1"
                     /translation="MKITTSSKKISTTVLISMTDVIFLLIIFLLLSSNFASQTGLPIK
                     LPSSTSSQRQAQQVLHVIYEHDASILLLDKRYTLMNLEPALKEKFVSKDQVVRLSAEK
                     QTELQKIITLMDVIRNAGFERIFVATERPEPGNAR"
     gene            42601..43323
                     /locus_tag="PLO35_00470"
     CDS             42601..43323
                     /locus_tag="PLO35_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOH78163.1"
                     /translation="MPDRYRQYLPLAISAGLHLVLLIITMLYYLPLMKREPWYEVSWG
                     EPVVEEQIVEEVSEPAAEQASLPMQSTPVKPVVRQAPPSVRPSAPLNPPKAEDIGPPR
                     LSESEVVEAPIYTPTEPAIKAPPSLRSNPQVTDALRQTVSGIPANNSPGSVESDLEGG
                     KLIRTDTKHRTHSFGDYGEVKLSFSVDMSARLIASSIRVIQSQNSKFDAEAISVLKDM
                     TFSFRGRPDPEKPYQITIKFSP"
     gene            43326..44159
                     /gene="folP"
                     /locus_tag="PLO35_00475"
     CDS             43326..44159
                     /gene="folP"
                     /locus_tag="PLO35_00475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOH78164.1"
                     /translation="MSANPLSQFKMPALMGILNLTPDSFSDGAMFNDPERALEHARSL
                     LEAGAGIIDLGGESTRPGALEVSVEEELQRVLPVLRKLGGLHPRPRISIDTRHARTAE
                     ACIAEGANLINDISALRHDPDMAPLLAGFPQVDVILMHMRGQPETMQVAPRYDDLYND
                     IKGFFIERIEFCEAHGIARERLFLDPGIGFGKTAEHNLALLGALHRFAELGLPIVLGA
                     SRKRFIDAISASAPDQRLAGSLAAALVGAMQGVAILRVHDVFAHNQFFKVLRAIVGEK
                     A"
     gene            44156..44899
                     /gene="cdaA"
                     /locus_tag="PLO35_00480"
     CDS             44156..44899
                     /gene="cdaA"
                     /locus_tag="PLO35_00480"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOH78165.1"
                     /translation="MNWLIPQFRDMVDIVIIAFLIYQALSIVRKSGGYQLLTGILLLF
                     LLYLASVLVNLKMLSAVLGAVRNYWILALIILFQPEIRSMLARFNLSQDMGIFKKREN
                     TNVFTTLVDAVSSMSFRKTGALIVVENKRKLNEYIQNGEPIDASISLRLILTIFNPRS
                     SLHDGAIIIRGDRILAAKVVLPLTKKSEYVKRFGTRHLAGIGISELTDAIAIIVSEQN
                     GHVSIAQGGVIQNNVAFEELMQIVSDAAG"
     gene            44903..45544
                     /gene="coaE"
                     /locus_tag="PLO35_00485"
     CDS             44903..45544
                     /gene="coaE"
                     /locus_tag="PLO35_00485"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOH78166.1"
                     /translation="MAAQSLPRPIIIGITGNIGSGKSSFCAFLEQAGLAVYYADLLAK
                     AVLRDPEVLSKLAARWGNEIAEDGKPRSEVIAAKVFGKPEELAFLNSLVHPGTLKQMQ
                     QLVDQSTTPVVLFEVPLLFEARLEDCFDYLVLITAPLELRTRRIIDRDKATRQQVGQR
                     AAAQLPDSEKASRCDLVILNDKGLKDLEEQALIFVRQLPELRTRPKRPFYSGN"
     gene            complement(45683..46669)
                     /locus_tag="PLO35_00490"
     CDS             complement(45683..46669)
                     /locus_tag="PLO35_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH78167.1"
                     /translation="MRKINLILLFLLALVPMLAAGPDMVPVVGGWIAVGLGADDQPIQ
                     VRVSDFQIARYELTLKEFREFVTATGYLSTAEIKGAGFGLVEAAWTEKAGLSWRDPGY
                     EQADDHPVASLTWYDAVAYCNWLSAESGLTPAYTIDKLNKDPNNQNEVDIKRWTVTWN
                     PEADGYRLPTATEWEFAASDRGMGYLYSWGDDDLLENPLLEPPANLADESHLEYYEQF
                     DDFPWGYMEGYEDGYAFSSPVGSFSPNFLGIHDMAGNVSELCWDWHSLDYQSENGSID
                     PKGPAEGSVHAQRGSAWCDALEESRIGYRRDLSTDYLSYNYIGMRLARNAVR"
     gene            complement(46742..47041)
                     /locus_tag="PLO35_00495"
     CDS             complement(46742..47041)
                     /locus_tag="PLO35_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78168.1"
                     /translation="MHKNYYDPFEPTPDLSLQPHRGRLVLLLGILGFFTVGIFGGLAW
                     HYGNLDLRKMNMGVMDSSGVDLTEYGRILGIISVCMWIIGLLVLAFWLATRIPAV"
     gene            complement(47100..47561)
                     /locus_tag="PLO35_00500"
     CDS             complement(47100..47561)
                     /locus_tag="PLO35_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOH78169.1"
                     /translation="MPYILAWIGIFLMVALAGTGSAMGTVMGGSASVGAMKKRDDIFP
                     YCLILSAMPGTQGLYGFGAFFILQTHISYSMNLLQASAVFAAGLIMGLVGLISAIQQA
                     KIVSTGIESLGNGNNVFTNTLILGVLPELYAIIAFATCFLIGGLIPGLVLK"
     gene            complement(47585..49366)
                     /locus_tag="PLO35_00505"
     CDS             complement(47585..49366)
                     /locus_tag="PLO35_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOH78170.1"
                     /translation="MIEKMRKYSFVLFHSDYESFLADLQRLGVVHIIRSTDARTDSQV
                     RQLELMGRYNDALKLLKKIGSDKEKGNTSLPTKALLNRIEQAVSEKDDLRRQEDILKK
                     QIRELSPWGHFDYELETQLENAGVKVDFHTCLKNHFKPEWLEQYCIRQINELGGIVYF
                     VVLYTGEKPALDCDTFSFHKHTLREFETQLEELQQRIADIDLFLNETAPEAIQRFEEE
                     IQRISGEYDYEDATLQASSEAEDHLRVLGGWIPVSKERELSTYLNDKGIIHFAQDGKA
                     DDNPPIQLRNNWFSRLFEPIGNMYMLPYYNEFDLTPFFAPFFMLFFGFCNADIAYGLV
                     FIALGLFLRHKAKNPAAKSVMMLIVIFGISSMLMGWLMGSALAFDLKKTVLDPAIIIR
                     NNDQIFNFALLLGAIQILFGVAINAVKKGRQSGFMHSVAPIGIFLFLGSLSVLGAEML
                     GTDISAVKAYVRFPMFAGLALLLLFNQPGKNPIINVLGGLWLLYNVITGFFGDILSYI
                     RLFALGVSSAILGFVINSIGSQMLGIPIIGPVIFFVFMLLGHALNIALGALSGFVHPM
                     RLTFVEFFKNADFQGPGIQYKPFGNKQ"
     gene            complement(49363..49962)
                     /locus_tag="PLO35_00510"
     CDS             complement(49363..49962)
                     /locus_tag="PLO35_00510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOH78171.1"
                     /translation="MTLKFQYNKISQLNMRKQLKIRENALPTLKNKESALRFEVKKAR
                     DAAHAMDLEIEARGARLEEFLKLWVEFDPSLIKISEVKIRSRKIAGVKTPELEGIEYQ
                     LSEYDLFTAPSWWLDGVSTLKELSRMQIEREFMVRKMQLLEVVRKKTTQKVNLYEKVQ
                     IPAYEEAILKITRFMEDEENLSKAAQKILKGRMAAEANA"
     gene            complement(49959..51281)
                     /locus_tag="PLO35_00515"
     CDS             complement(49959..51281)
                     /locus_tag="PLO35_00515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOH78172.1"
                     /translation="MATTAFQKVYTKLTQITKATCTVKATGVGYEELATVSGRLAQVV
                     KIIDDNVTLQIFSGTEGIGTDAEVLFFGKPPTLKVSDELSGRFFNAYGDPIDGGPQIE
                     GREVEIGGPSVNPVRREEPSELIATGIAGIDLNNTLVTGQKIPFFADPDQPYNQVMAT
                     VALRTPADKIILGGMGLSNDDYLYYKNAFENAGMIDKIISFVNTTDDPPVERLLVPNM
                     ALAAAEYFAIEKNEKVMVLLTDLTLYCDALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPTGGSITIIAVTTISGGDITHAIPDNTGYITEGNLYLRRDTDIGKVVIDPAR
                     SLSRLKTKVMGKKTRADHPKVMEASVRLNADATKAKTKMENGFELSDYDDRTLKFAYD
                     YAMQILAIDVNIDTDQMLDTTWELFQKYFTREEVAIKDEFMNLYWKKA"
     gene            complement(51281..53038)
                     /locus_tag="PLO35_00520"
     CDS             complement(51281..53038)
                     /locus_tag="PLO35_00520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOH78173.1"
                     /translation="MMTKGIVKGIIANLVHVEVDGPVSQNEICYIDMDGVKLKAEVIK
                     IQGNIAYTQVFESTRGLRPGYPVEFTQRMLEVKLGPGMLSKIYDGLQDDLEKMEGMFL
                     NRGEYTDPLDEDSSWEFTPIAKPGDSLSAGDWLGEVPENWISHKIMVPFTMEDKYTLQ
                     SIAEKGTYKLTDTIATLADTEGKTFEINMVQYWPVKLPIRAYKEKPRPFKMMETGVRT
                     IDTLNPIAEGGTGFTPGPFGTGKTVLQHSISQNAEADLIIVAACGERANEVVELFVDF
                     PKLDDPRTGRKLMERTIIICNTSNMPVAAREASVYTAMSIAEYYRSMGLKILLLADST
                     SRWAQALREMSNRMEELPGPDAYPMDLPAIISNFYSRNGYVYLNNGVPGSITFIGAVS
                     PAGGNLKEPVTEATKKAARCFYALSQDRADSKRYPAVDPIESYSKYLEYDEVQNYLNK
                     NITSHWVADVYEAKDILLRGKEAGDQINILGDDGVPMAYHERLWKSELLDRIILQQDG
                     FDVVDKSTPMKRQSYMLEMVLGVCRTDFVFDSYEEVQPYYTKLINILKQMNYSEFHSS
                     AFKGYEAQLQETMDERRAK"
     gene            complement(53048..53935)
                     /locus_tag="PLO35_00525"
     CDS             complement(53048..53935)
                     /locus_tag="PLO35_00525"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOH78174.1"
                     /translation="MSSGQYYYFVAGLPRIGIDDAKLQIEPEAFLEQAATQLTDDDLK
                     LLKLLTLEDGIKTLSALLFKQEAPPDRPGYYEAAFWSEFGEFARLRVENREAPVPKQF
                     KDLPPFLLKRCVEFLSLEEPPARIMVEHGFYQDLFSFAAAHSNRFVSAWFEYRRQSRN
                     VVLAINGRAHELDYARWLIGDDELNDKLAHGRGVDFGIGKSSPVFDAHLRAYEQNSVL
                     YRERAYDILSWKWIDSHNFFQYFSIDRVLGYFAQLLILSRWMRMDVNLGKEVFYDTLN
                     TLQNSFSFPPEFAIKQKNK"
     gene            complement(53940..54539)
                     /locus_tag="PLO35_00530"
     CDS             complement(53940..54539)
                     /locus_tag="PLO35_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOH78175.1"
                     /translation="MTDQLQDLLQRVHEEGVAKAKAEADKILQAAQQTADDTISKAQA
                     QADKIISEARGQSTELAKNSETDLKMAAQHTISSVKQRLTEVVLQKVLDDPIKAHFSD
                     PAFLKQIILSTLEAWKSSGGSITIAETMRGKLDDALQGSLAEALKNGLKVDYSPAVKN
                     GFTLAPADGGYKLSFTDEDFANLFKSYLRPRTAQLLYSE"
BASE COUNT        12772 a        14346 c        14510 g        12848 t
ORIGIN      
        1 gcctcagcac cgcgtcccac accgaaaccc gcaagatgct gctgctgaaa tagtaaggtg
       61 catgtatgtc ttgtctcaat caaagccatt cctgttattc cctttttctt gcggcctaca
      121 ctttcaggat tgttgcataa acattgatta cagctatagg aagcctgcgt ctatcatgac
      181 ctctcacaga agtgatatac tacacggagc aggattacac tactatcatg tcgatagttc
      241 gggataggat gaggttgggg aaagagatat tgacaataaa atccgatcag agaatcatgg
      301 ctttgcggta tttgaattga ttatattgat ttggaaaaga cagtctatct gctcataacc
      361 tataccaata gaggaattta tatgaataag atttacgtaa caatggctct agtgacaatt
      421 ctatcgctcc ttacgatagc cttgccagcc caaaaccaca tgactctggc aatgagtatc
      481 ttcggggatc aggattacga ccactttgga gacaaactgg catccctgga ttttaacggc
      541 gatggttatg atgacctggc ggtgctacaa tcctggtggg tgccggacag catctatgtg
      601 accatcccct attctgtaag cgcgggaaga aactggggcc ggatcctctt ctattatggt
      661 ggcccaggat tcgatggagt ggccgacttc acgatcgaag ccagccgaca ataccagttc
      721 catttttcaa aaagttacat gttcagcctg ggtgacgtca atggtgatgg ctatgacgac
      781 ctggggatcc ttgaacactt tcccaagcgc ctggacatct tctttggggg aactaatccc
      841 tcctcatcac ctggatatat gattgatctg caagatacct ccatttacca ggcgcagttt
      901 tacaaactgg gagatatcaa tggtgacgga tatgatgatt tctggctttc gacatatcct
      961 tactccacat acacaggcaa tgtatcgata attttaggag gaacctttca gcagatcatc
     1021 tttgacagta cctcagaagc tgattatagt ggatttcatg caatcggtga tatcaaccac
     1081 gacggttatg acgatatctg ctacacgatt tttcgtcagc gtccaaccag atttcacagt
     1141 aagactgttt attatggttc gccggatctg aacctgcaaa atcccgtggt cattgttccg
     1201 gattatacgg aggcagccgc ggtcgtagcc aaagcggtgg gggacgtcaa cggcgatggt
     1261 tatgatgatt ttattggact gatgaccggc tacggacaga ggctctggtt gggggggggc
     1321 acgatcggcc aggattggga tgttctgctc aatccaccct atggaggtag caactccgat
     1381 cactgcactg tgagcggaga cttcaacggc gacggcttca gtgacattat cggagccagc
     1441 tttaaccttg agtgtgcggc cctctggctg gggggcagca atatgaacgg aactccggac
     1501 ctgtttctct atggcatctc cggcggaatg caatatggcg tgagcatggc cagcggagat
     1561 ttcaatgccg atggctttga tgacgtgttt atcggtgaac cgagcctgag taatgatata
     1621 tttcacggca ggatcaggta ttataccggc aatggccagc ttgaggacac cacagtagct
     1681 gtggatgatg caataaatcc accctctgtg agcaattggg ggttcagggt aattcccaat
     1741 ccttccaaac aaggcacgga tctcagaatc aggttttacg gagaaggatt tgacaggctg
     1801 ctccatgccg aactgcgcgt ctataacctc agaggacagc ttgagtacac ccagcagata
     1861 tcaaacgctg cactcaaaag cggcgatgtc gcaatagata aactccctct tccaagggga
     1921 gtgtatctgg tagccatcta tgaagagggc caacgactca aaacccagaa agtcaccatc
     1981 aaatagggag tgatcatgag aacctatatc catatcttgt tgtttatatg catactggca
     2041 gtgccatgca gcttgcctgc cctgtattct gatgattttc tgattggggc atattcccat
     2101 atcagaagct actcaacctc tgccgcctgc acagatgcca tagccaccag ggctagggaa
     2161 gcgggattca atgccctgac ctcttacacc gcctgggaga ccaatgacac cgcaccaagc
     2221 gacatgttcg gcatcctcga cagcgactct ctcgacgtca tatacgaaga cttgatcttc
     2281 aatcccgatc ccgatcccag cctggaaaag tatggctacg ccgccctctc gtccggaatc
     2341 gacatgaagt ttgagtctga atatgccgac gcttcagaag ttaactctga agacagcttc
     2401 ttggacacct tcttttacat gtcctcatcc gcagcgaggg ttgggagctt tggatacgat
     2461 gaagattaca gtaatgacaa ctactggtca gttccagaca gcagctccgg ttatgcctac
     2521 aaggacataa cctacaggtg gcctcatggc gctcattatg acagggtggg caatgaattc
     2581 cggtttctgg gatataccac aaatggtatt gatactcctg aaaacaacaa actcacagtc
     2641 agatatgtat ttcgcgtcga tgatctgggc agctacagcg attcagacac tctggcggtg
     2701 ttcagttttg taaaatatga tggaatgacc acggaattca ctgtccacga cagcctctac
     2761 ttctatcagg ggtccgcttt caccaagaag tatgtgctca caaaagccat ctacgagtcc
     2821 ttaccacttt ctgatacctc ttcactcacc gggtgtaaag cgctggaatt tcagtttccg
     2881 ttgaaaggat cattcgcgca cacactcgat agccagggca tgttatcaga caatgcctgg
     2941 cacttgcaga tgaataaaag tcttaacccc tggatgtatt ggtgtgggcg cggttcgcta
     3001 aggctggact acattgaata ctatgacgac gttttcgcaa actataccaa caatcctgcc
     3061 aaacgtgcca aactgcgtaa cttcgagagc gtagccaatc ttcgtcattt ttatggtatg
     3121 gacgaaccca aagctcccaa cttcgagtca ttcaaaaaac tgagagagca tctgcaggca
     3181 acattcccaa tggatgcaag atacattgtt acggcaaatg acctggatcg ctggcatctg
     3241 gaaaaacctg acacaacccg ctaccggcgt tagcttgcta caaaacatga acggcacgga
     3301 tatccaacag tgtagcttcg ccaacaccat aaatggcaaa caaggcactg gtattcatct
     3361 tactgcagca tcaccaacga tttctacgtg taactttact aatctatatc gtgggatact
     3421 cactgagttt tccctaacaa gtggggtagc gctggaatca agtgttaccg aatcaaactt
     3481 caacaactgt gaaatggggc tagagagtcg aagctcttat catcggctga aggcaaacta
     3541 cttcaatcgc aataactcaa gcattgtcaa tcacacaggt tctaatctga acctcagtta
     3601 cgatgcgaat aacgtcttga tgaaccataa tgccaatatc gtattttacg ataccatgcc
     3661 ctgtgagtct acgattcagc tcttcacggg acacaatgac ttctatcatc taacagatga
     3721 ttcaacgagt ttagatgcca tagatttcag ctttgacgcc aactattttg atttcccatt
     3781 gacactggat ttctcaatca atgccagcaa aaactggttc caggacgatc aggtgactgt
     3841 taacaatcca gcctatgttg actttgtgta tgttgattta tatgatccat catcatcgat
     3901 gcctgctcca tctccagaaa atgatagact atttatagct cttgagtatg agtctcagga
     3961 attgtacgaa ttggcgtgcg ctacctatca ggctatcata gatgatcaat tggaagaaga
     4021 gcaaacatac gttaccagtg ccattgatgg cttgtatcgc agtacatata tgatccctga
     4081 tccagcttgg gaactgactg actatttcga tacgaaggct cttcaatatg ctattgatga
     4141 tccagccctg agtgctatac tcaatgatta cctggcaaaa gtgtttgtgc tggataagga
     4201 cttccaggct gcagttgatc ttattcaact acgcatcgac accccgatta gcgaaatcga
     4261 ttcactaaga gctgtcctgg atctggagat agtgcttcaa ctggcggcaa tggaggaaga
     4321 taagcgtccg cttacgacaa aatatgccca ataccagtat cctgatatcc aagtgtttga
     4381 tgtgatgcat agccacaact ggaataagta caatcgcgcc ttacaccaga atgatcaaga
     4441 aaccaattcc gtaatagcgc caacgcctca aattcaaagt aattacccta acccgttcaa
     4501 cccgtcaaca acgatagcgt tcagtatccc ggaaaccgga cgggtcagag tatctgtcta
     4561 taacatcaaa ggtcagaagg taaaggactt gctcaatact gaattgacca ggggaaatca
     4621 taggctggtt tgggatggca aagacgctaa tagccgtaac gtagcatcag gcatctactt
     4681 catcaagcta gaatcgggag gaaaaacctc gattcgcaag gcaatgctta tgaagtgata
     4741 aaccgagcag gagctagatc tgctcctgcg ccctttacta aattcgatgt gttcaggcct
     4801 cacttagcca cataaaatct acttcctcac ctattgcttg ccgtagagat gccgtggcgc
     4861 cggcggcgct tttccccaga gagagcacgg catctcaaga gcggctaaag agcaaggtaa
     4921 atgagcgggt gaatcccgga gatattgatg agcttgaggg agttgaggaa cgcgccggat
     4981 tggaagccga cgcttcaacc cacgcaaaag aggacgtttt cgcctcctcc cttattgatg
     5041 ggacgggtgg ctgtccagag ctcgtcctgg gcatccccaa taaagttcgg gatgggtagg
     5101 gcgagctatg ggcagccagg agagatacga ataagggcgg actgaatgtg gaattccggg
     5161 tagcttgctc gagaggatgt ggcgcaagct ctggcttgaa ggggcgaata ttgtatctgt
     5221 ttcggctcga aaattgtatg cccatcagga aatgtaatgc aataaattgg agctaaacac
     5281 gatgaaccga atccagccgc ttgtctggct actgcttttc gcgatggtcc tgccgtttgt
     5341 gacgcttggc gctcatgccg ttctgcctgc ggacacgatg gaccacgatc tgcgtttcac
     5401 ggagaccaat ccacccgccg cgccggtgag gcctatagcg gaatttgagc ccgcttccca
     5461 tgtcctgatc agatacccgc ttggtattcc ggtcgctttg gtggcgcacc tttcaaacac
     5521 cgctcaggtg atctgtctcg tatcatccac atctcagcaa aacactgcca ccaacacctt
     5581 taacaacggc ggggtcaata tgagcaacgt cagcttcatg atcgcgccca cggattccta
     5641 ctggaccaga gactttggcc cctggttcat tatggatggc aacgatcagg tcgcgatcgt
     5701 ggattttgtc tataatcgtc cccgccccaa tgataacatg atccctcaga cctttgccac
     5761 tcagcagaac tttacctatt atggtatgaa tgttcagcag actggtggga attatatgtc
     5821 cgacggcatc aacaccgcgg cccaaaccac tctcgtatat gaagaaaaca cgtctctggg
     5881 ccagaccggc gtgagcaaca aaatgctgca atacatgggt gccagcaact attacgtgat
     5941 caacgatccc aacaacacct acatcgacca tatcgactgc tgggccaagt ttttagctcc
     6001 cgataaagtg ctggtgcgca gcgtgcccac ctctcatgct caatatgccg ctatcgaaca
     6061 ggcagccaac tatttcgcca cccgcaactg cgcctggggc tacccctgga aggtttatcg
     6121 ggtcaataca cctcaaaatc agccctacac aaactcgctg atcctcaatc acaaggtctt
     6181 tgttcccatc atgaacagca catatgacgc ggcggctttg caggtttacc gtaacgcctt
     6241 gccaggttat gaggttatag ggatatccag cccctcttcc accccctggg aatccacgga
     6301 cgcgctgcat tgccgcaccc atgagatcgc cgacaaacag atgctgcata taactcacat
     6361 gccggtctgg gggcagcaca atgaaagcga gaccatcaac ttcaacgcca gaatcaaggc
     6421 ctacagcggc caggcgctct acagcgactc tctgaaggtg atgtggaagg tcaatcaggc
     6481 cccctgggaa tccaccctgc tgcaagacct gggctccaac aacttcacgg ccatgctggc
     6541 aggcttcgcg gctggggaca cggtgagata ttacatccac gccgccgacg cttcaggacg
     6601 ttccatcgat cacccgctca ccggagctca tgatccccat atcttcagtt tctatcccga
     6661 tcaggtgcct cccgtgattc aacacacgcc gcccgtgagt gtgatcaatc aagacagcgg
     6721 tcccatcacg ttttccgccc aggtgagcga caacgtctgg gtgcagcagg tgctgttcag
     6781 ttattacagc gagacgcagc ccctgctcag tattcccatg acagacgcgg acgggattta
     6841 cacgtttgaa tactatccgg agtttaccga ggacgatcat tttctctggt atcagatcac
     6901 tgccacggac actgccaacc ctcccaatca ggctgtctat cccgccccag gagaatggca
     6961 aagcattcca ttgatgatag ttgacaatga tgatccagtc atcccagagg tggatttggt
     7021 catccagagc gtctatccca atcccttccg tccaactcgt gaaagcgctt ccgtagcgtt
     7081 taagggcaaa gcgggggaaa aagtttcgtg gagtattttc aacatcagag gccagaaagt
     7141 gagtgcggga ctaagcaccg tgaacagcga tggcgatggc cggatctcat ggaacggaaa
     7201 aggcccggat ggcagtttcc tgccagcggg gatctatttg ttgagactca gtcagtcggg
     7261 ttctcaccag agccgtaagt tgataatagg tctttagggg ctgtggaaaa tattctacag
     7321 gcagattgtg aaaaatacca caaattccgc ttgacagaaa aacccgtgct tataaacatg
     7381 gcctcagcct acagacccta catagaatgc cctccgagca gtggggatgc cgcccggcac
     7441 tccccactgt tattttttta cctgtaaaac aaggagttat gtgcatgaga acgctctcat
     7501 ttcccgtagt tttgatgatt gccctgatca tccttccctt taatctgtct gccctgagat
     7561 cagtggcgcg gcttgataat ccccctccag ggatggcaga agattgcgac gccctgggtt
     7621 atgacgtggc gtcatatcat ccgggagttt atctcgatct ggtggttgac gaggccgaac
     7681 tggccgcttt aaaactgcaa aacggctcct ggtatgtgac ccagacggaa gctcagctgc
     7741 gggaggccct tactgcgcgt agagatattc ccggctaccg ggactatgat cagatggtga
     7801 gcgaactgat gcaactgcag gccatttatc ccaatctggt gcaggtttcc accatcggga
     7861 gcggatgggg cagcatctat agccagaccg ttccatatta cactgggttc gatcatgacc
     7921 tctgggccgt caaggtttcg gccaacgtgt ctcaggaaga ggacgaaccg gctttttact
     7981 ttgtgggcga acaccatgcc agagaaccaa tctccacgga agtctgtatg gagattctga
     8041 tccacttggt ggaaaactat ggcagcaatc ctgaagtgac ctcgtttctg gataacagcc
     8101 aggtgtggat cgttccgctc ctcaatcccg acgggcataa gatcgtgatc gatcagaccg
     8161 acgtatggtg gcgaaagaac atccgcgaca acaacaacaa ccaggagttc aacaccgacg
     8221 actcttcagg aatggggccg gatggagtgg atatcaaccg caactatggt tatcgctggg
     8281 gatatttgag cgctacggac aatatgcaga tgcccaccta tcatggacct tatgcttttt
     8341 cagagcctga ggcatccgct ttcgcggatt ttatctcgtc ccgacgtttt ctggcgggcg
     8401 tcagctatca cacctatggt cagtatgtgc tctatcccta tggtttcgta ggcgatatca
     8461 acgctccgga caaggtggag ctgcaagctt tggcgactga tatggcaaac gtgatcctca
     8521 aacagggaag cactacccag cattacgacg ccatgccttc ctggcagctc tatccggtga
     8581 gtgggagttc ggacgactgg atctacggta cgaccggcag ctttgcctat acgattgaga
     8641 tggccacgca gtttattccc aatcaaaatc agatgcagca gatcatcacc caaaacctca
     8701 acggggcgat gatgctgctc agaaggtccc agaaaaagat gctgacaggc catgtgacaa
     8761 atgccgagac cggggcaccc atccccgcca gagtgtggat cgacggttat gacaatcatc
     8821 ccctgggttt cgcacccatt tgcgccgatt ccacctttgg cagatattat cgtctgttac
     8881 cgcctggcca atacgaagtt cgctattttc tgccaggctt ttaccctcag accagaaccg
     8941 tagtggtgac tgccgatgcc gtgagtgtgg aagatgtgca gctgacaccc tcccagccca
     9001 tggaattgtt tatccaggtg aggggtgact tctttggcgg agtatccgag gctcagttgg
     9061 tgttcgatca agatccagaa cacgtttata ctaccgattc ggctggctgg atcatcattc
     9121 cggagttcta tcccggcgag tacaacgtca ctgtcagcaa accgggttac agcactctcc
     9181 gggtcaccag aatgatcgag accacctcga tcggctttaa actcagcagt atggccggct
     9241 ttgacgatgg ttttgaaaac ggcatgggaa actggaacac caatggcagc tggggagtga
     9301 gctctgccca ggtttacagt ggggcatccg ctttggcaga cagtcccgct ggcaactacg
     9361 gttcggacat cagcaaatgg tgcaggctca gccagccggt caatctgacc gcggtggaaa
     9421 acgccgacct gcagtttcat atcagccaca acctctccaa cgatggtgac tattgcctgc
     9481 tgcagtacag cctcgacgga cagaactggc tggtattcga tagttttacc ggggtggcgg
     9541 cctggactct caagagctac agcctcaatt ctttcatcgg ccaaagtgta cacttgaggt
     9601 ttctgcttgt gacgaattcc agcgcgaacg cggatggcgt gtttatcgac aacttccgtg
     9661 tctatacagg ttcgctgctt accgccagcg acgatctcct ggcatctgct gtgaaactgc
     9721 aaagctggcc aaatcctttc caggacaagc tcagcttgat ggtctcaggc ctcaggggcg
     9781 gtgaagccga tttgagcttg agcgtgtata accttagggg acagaaggtt tggcaacgca
     9841 cggggctgtc cgctaaagac ggatatctgg acctggagtg gaatggactt gacagccagg
     9901 gccgtgctgt ggcttctggc atttacgctg tgaaattgac tgctggcggt aaaacgctgg
     9961 ccagccgcaa gatcatgaga gtaaagtaag gatagaacgc gccgaagtgg aatccgacgc
    10021 tacaggagct cgtggattgg aagagatcag aatccagaac atcagcaagg aattcgatgg
    10081 attcctggca gtggacagtg tggacctcac gatcaaatcg ggagagctgt tttcattttt
    10141 ggggcccagc ggttgcggca aaacgactct tttgaggatg attgccggct ttgagattcc
    10201 cctgcggggc aagatttata tcggcgatca ggacatcgga gacctgcctc cctacaaacg
    10261 gcccgtcaac accatctttc aaaactactc cctctttcct catatgacgg tgtgggacaa
    10321 tgtggccttt gggctcaagg taaaaaaagt ggcgcttccc gagataaaga cccgggtgag
    10381 cgagatgctg gaactggtga agatgagcga tcagtcttcc aagtatcctt cccagatcag
    10441 tggaggtcaa aaacagcggg tagccattgc cagagctctg atcaacaaac ccaaggtgtt
    10501 gctgctggat gaaccgctgg ccgctctgga cctcaaactg agacagcaca tgctgatcga
    10561 actgatgaac attcacgacg ccgtcggcat cacttttatc tatgtgactc acgaccagag
    10621 cgaagcgatg agtatatcag accgggtggc ggtgatggac cagggcagag tgattcagtc
    10681 cggccctcca gacgatattt atgagcgtcc cgccagtatt ttttccgctt atttcattgg
    10741 tgagaccaat ctgatcaccg gagaagtgac gggtatcgaa gatgaatacg tgcagctcaa
    10801 atgcccgatg ggggatgacc agcacttcga gatcgactcc accttccact tcagaccgga
    10861 gctggggcag tctctcactg tgtccctgag accggaaaag atcgccatat cacgttccaa
    10921 accacgctat caggatgaca tcaacatgct gcgtggcacg atcgaagata tcctctatct
    10981 gggcagtcac actcagtata ttgtcaaggt gggcaagctc aaattcaggg tcttcagtca
    11041 gcataaacgg gtgtactttg acgacaaagc gctggactgg gatgagcagg tctggctgtt
    11101 ctggcatgac acggacacct ggctgatcaa cgagatgcct tccgatgagt ctcgctctcc
    11161 cgagggaaca tctcatttgc agcgctgggc aaaaagagac ctgaaccgtg gcgcacaggg
    11221 agatcagtaa ctgatgccca gacaggcaga tccggcaagc ggatcacccc gcaggaggat
    11281 ggattatctg ctggcggccc ccacgctgct gtggctgacg atcttctttc tggtgcctta
    11341 cttcatcgtc tttatctatg gatttctgac acccgatatc ttcgacgtca aatttgagtt
    11401 ttctctcgac gcttaccggc aaatcttcaa cggtacgtat ttcaagccct ttttcctgtc
    11461 tttccggctg gcattgctca caaccatgct ctgtgtgctg ctgggctatc cggtggccta
    11521 cttcatcgcc agagctggag acaagctcaa aaacacgctt ctggtgctga ttatcattcc
    11581 cttctggacc aatctgatca tccgtatctt cagctggcgc atctttttat catacaacgg
    11641 cgtattgaac gactttctca tcagcactgg gctgatatcc caacccctgg agatcatgcg
    11701 gaccgatctg gcggtgattc tggtcatggt gtatgtctat ctgccctaca tggttctgcc
    11761 gctcttcagc gtgctggaaa agctggattt cactcttctg cacgcggcca tggacctcgg
    11821 cgcgaacgaa gtgaaggcct tttttagggt aactctgcca ctcagtgtgg gaggcatttt
    11881 cgcgggagtg ctgctggtct tcattcctgc cctgggagcc tatctgatcc cgcaactggt
    11941 gggagatcag cgctctctct atgtgggcca ggtaatcacc tataaaatca agaacatacc
    12001 ccgcaactgg cctacggctt cggcgctgag tttcacgctg ttgttgctgg tggcggtatg
    12061 tctgctgatc ggctattcca tgtatcggaa ccgccaaaag gccaggcgct tctgatgaag
    12121 gctttcaggc tctcttcgct gtttaaagga ttgaacctga cggtgttcac cctcgtcatg
    12181 gtgtttctct atatccccat agtcatactg gtgatcttca gcttcaacga tgcccgcatc
    12241 gtgaccagct ggaaaggttt tacgctcaag tattacgccc agctctggga aaacgtggcc
    12301 atccggcagg ctttctggaa cacgatgctg atcgccgggt tcagcacggt gatttccact
    12361 ctgctgggtt tgctcacggc cctgggcctg gaaaacaaaa agctccgagg ccggcgggga
    12421 ttcatggccc tgctttacgc tcctctggta attcccgata tcctgatggg ggtatcactg
    12481 gctctgctgt ttaactttct gagagtcaac accggcatga tgactgtcct cattgcccac
    12541 atcactttct gtgtatccta tacggtgatc gtgatccaga gcaggctgga ggattttgac
    12601 tattcgctgg ttgaggccgc catggacctt ggcgccaagc ccaatactac cttcttcaag
    12661 gtaaaactcc ccttgatcat gccagggatc attgccgcgg ccttactggc ttttacgctt
    12721 tccatagatg acttcattat cactttcttc acttccggcc ggggctttga cacgctaccc
    12781 atttatgtgg aaggcactat ccgcaggggc accattacta ccatcaattc gctctcgacc
    12841 ctgatgatcg ggttcacact gtttctggtg gtctttacca agcggattcg taaatccatc
    12901 atttccttct tctaaaaaag ccagtctatg gctggttttg agagcggatt atcccaagct
    12961 tggaatggat attgcaaaaa acacctttca gtccgtatag gccgcttagg agcggtctgt
    13021 ctgatgtaat aagtatttga ggaatagatg agtgtattga ttctggccac ggtgctcctg
    13081 gtcctgaact ggatctggga ataccatcgc catctgcgca atgtcaagag catcccgatc
    13141 cgcatccacg taaatggcac ccgcggcaaa tccagcgtga cccgactgat cgccgccggt
    13201 ctgcgagctg gagggatcaa gactctcgcc aagatcaccg gcaccttgcc acgcgtggtt
    13261 ttgccggatg gaagggaagc cgctatcatc aggttgatgg gagccaatat cattgagcag
    13321 aaatacatct ttagagaagc gatagcccac aaaccccagg ccgtggtgat tgagtgcatg
    13381 gccgtaaatc ccgtcttcca gtggatcacg gaacgcaagt ttgtgcattc caccatctcg
    13441 gtgatcacca attgccgccc tgaccatctg gacctgatgg gctccaccga acagagtgtc
    13501 acgatgtctc tctccaacac gattcccgtt aatggcacct gctatacggc tgaaaacgaa
    13561 cactataagc tgttggaaca cgtagccaag cgccgccact gcaagatcca tcagatcagg
    13621 ccgagggacg ttacccacga ggagctggct gggttccgct atattgagca tgccgaaaac
    13681 gtccaactgg ccctggcggt ttgcgccgaa gcgggagtac ccagagaagt agccctggaa
    13741 ggcatgcggc acgctcatcc tgatcccggg gccctcaaga aatacatcgt cgaagacagg
    13801 gataaaacca tccatttcta caacgtattt gccgccaatg atcccaagtc cactgcccgc
    13861 gtgatcgata tggtcacggg ccatctgaaa ggggtggaaa agatcatcat cctcaacagc
    13921 agatcggacc ggctgttccg cagcgagcaa ctcgttgatg ctgtcagaga gcttgatttc
    13981 agctaccttt tgctcactgg cgagattccc gacaaggtgg aaagctatgc cctgcgcacc
    14041 gggatcccca aaaacaaagt cttcccgctg ggagagcccc tgcccgaagt cgtttatcaa
    14101 aaggcctggg agctcaccgg gaaggaatct cacgtgttgg gaattggaaa catcgcaggg
    14161 cggatcaaat atggagctca gatcgtagct cattttaaac acaagattca ggctggcaag
    14221 aaagccagaa aacaaggagc cgttagtggt tgacgcagta gtacaggccg cggttggcat
    14281 aggcgttatc atcagtctca tcttcagtga gctgttgggg gcctcagctg gcggtatcgt
    14341 agtgccgggt tatgtggcac tctatttgga taaaccgatg cagatcctgg ggactctggc
    14401 tgtgagtctg ctcacctggg gaatcatcag gattataggc aagttcactc tgctgtttgg
    14461 taaacggcgc atggtgctca gcatcctgat cggtttcatt ttgggctggg ctacccgcat
    14521 gctggtgttt gaaaaccaaa cggtctatac ccttcagatg caatcgattg gctatatcgt
    14581 gcccggtttg atcgccaact ggtttgagcg tcagggtttt tggaagacca tctccacaat
    14641 gggcgtggcc gctgtgttgg taagactgac cctgatggtg gtctttggag gcgaagtatg
    14701 agcatgtaca ggccctctct taaatccggc tggtcgctga tcctgttgtt cgtatttgcc
    14761 gtgggcctct acgttttggc ccaatccagc tatgtgcaca tcaagaccga taactatgaa
    14821 gaaaaagtgc aggccgctcg tttgatgcag acctatatgg acagcctcaa ggcggaactg
    14881 aaggccacgg gctatgaatt tgatcccata gacgatccct ttcacacagg tctgatcggc
    14941 acccgtcttt catccattac cacagacaga ggtttgcttt cagataaaca agctgccctc
    15001 aatcccaatc tggcagccat tttcgtggaa gagttcaaga aggaaaggct ttcagagggc
    15061 gaccatatcg cggtgggaat ctcaggttcc aatccagccg tcaacttagc cctttacgcg
    15121 gccatcagtg tgctgaaact caatcccgcc gtcatcacgg cagtttcttc ggcttcttat
    15181 ggtgccaacc gggaagatta tacctggctg gatatcgagg ccgtcctgaa aaagaagcag
    15241 ctgatcgact tcagcagttc ctatgcctct ctgggaggta aggatgatct cgcgatcggg
    15301 ctgtctgaca acggtctcgc cagcttgaga gatgccatga cccgcaacgg ggtcccactg
    15361 ctgctgggct ccactctgga agagaatgtc tcgctccggg aggccgccta taaagaattg
    15421 ctgccggaag gacagcgata ccggatgttt gtaaacatag gcggcggctt ggccaacgtg
    15481 ggctcggaac ccaattcacg cctgatcccg gaaggcatca accacaaact ggccgagaaa
    15541 gaatttgaga aacagggcat catgatggtg atggccagac aaaacgtgcc agtccttcac
    15601 gtgcgccgca ttcaacgctg ggccagaaag tatgaggtgg cctccaccca ggaaaagatt
    15661 cccatcccag gagaaggacc tgctttcagc agccgcaaac acaacgtctc catcgcatcc
    15721 atctgtctgg ccctcctggt ggcagccatt gtggtcgtaa tagtcttcga cagacatgac
    15781 cgccgtttta tggccaacat cgtcgatccc gatgaggagt tgtaacagat gttcagaaag
    15841 ccgcactcac ccacaaatct tctctatcca aacccccatc ttctcaatgg agcaaaataa
    15901 tgcattttaa acagatacta ccgattgtcg ccatcgccgc catgagtttt tccgctctgc
    15961 aggccaagcc gaaagcgctc aactttgaca accccggaaa caaacgtctc ctcaagaccg
    16021 aagccggtaa ttattactac tatcgctccc tccccgagaa aagcatgaag ctcaacgtgg
    16081 cgggcatcga caagctggag ctacgctctt ttgccattga gccactcaaa aagcctcagg
    16141 tgatcagcat tgtcgccaaa aagcagacag ttcatgatct cagccttaaa agccgcctgg
    16201 atggatacta tctctatgaa agcgtgacta ttccaattcc cgaagggata aaagagctgg
    16261 aaatcctttg ctatgaacgg agcatctact ttagagcttt ctggaccgtg cctcccaaac
    16321 cgaagccggc acccaaacag aaactcccca acctcagcat cgatgcccac agcggcatac
    16381 tggccatgac ccacaacggc agcagcagcg attaccacag ctttacgcct gaccagcctt
    16441 tgaaattcac cctcaataac ggccgcgacg cagtcttata cgtcagagcg agactcctgg
    16501 accggaccct gccaatcttt gaagtgtatg tcaatggcac ccttgtgcaa acccatgaat
    16561 tcacgctcaa gcgcagcacc aaatactccg tgaccgggat ccgccatctc tcgatcggca
    16621 tgaaagtgga actgcctgcc aatcaaggca aatccgtgat cgaactgaaa gccaagagcg
    16681 atcatctctt tttgggacgt ccagtcctgt taaaagctaa ttgaggctgg caatgctgca
    16741 aaaacatcac cactctcccg agcgtgacaa acacccggac gccgctctgg aggagaagga
    16801 tgtcctgatc caggatggcg agcaggaaga aaccaagcag gatgtcaagc ctggcagcgt
    16861 gaggacatcg cgtaactttt tgccgatcat catgtcctgg tgcctggtct tcctctgtgc
    16921 ctggctgcct ctcagcgctc aaatcgaggg ggacatatca ctatcgaccc agtattccga
    16981 caacgtcttt cagctctcgg aatatgatct ggaccgcttt gaccaggaac atcccaacct
    17041 cggatatgtg gatacgacgg atgacctcag cctcaaggcc caggtggacc ttagctaccc
    17101 tttccgccac aagtggtacc gcttggtgcc ttccatcacc gccagtttcg atcagaatgt
    17161 gtccaatacc gataagtggc gtcgggatat tattgcccgc tttgccgtga tccgttacta
    17221 ctgggatttc aaggtgatgt atggctttta tcccaacatc cttgtgcgcg cttatgtgga
    17281 tgaagacggc accggcgaac tggagcacta tggctatcag cgcaatctct acagggctga
    17341 tctgagcgtc aaacccttgg aaaagacaac cctgcgcgcc cacttccgat acgaggaact
    17401 cctttacaac caattcttta ccgagtttga tggagacgcc aaaaccggcg gcatcggcat
    17461 ccgccaaaat ttcccggtct ttgtgctgga ggcgggctat cagtatcgtg tcttcgacaa
    17521 ctgggaaaac ggccccctgc aggatgactc ctcctacgag agcaatatct acaccgctag
    17581 tctgaggctc aaagacatgc cgctcaacga cggcaaatca tcctcccccc gctggcatcc
    17641 gtctctctcc cttagctttg aagagcgctt ctttcaggga ttggatgact ggtatggcgg
    17701 ccgggtggac aaaatttaca ccaccagcgc gggacttagc ttccgcctga acaaaaaatg
    17761 gaatatatca cttgactatt ctcactatct caggaatgta gattcaccca acgcttccgt
    17821 ccgtcggctc aagccataca gcgaaaaccg gctgggagcc gaggtcaaat acagcctttg
    17881 aggtacagtt cagatggttt tcaagcagga aagctcgacc aagaagatca aagaattcct
    17941 cgacctcgtg gaaaaccaca agatcgagct gagacacttt aaaagcccgg tcctcgtgtg
    18001 ggccgtgcag gatggcaccg ttcactattc cttttgggag gcttccctcc tggatcaata
    18061 cggtctggac aatgtggacg gctgggacta tgaagaagac gccctctttt gccccgactg
    18121 ggtgatggac gaggaagacg aagacctgga tgacgacgat gacgacgacg atctgggcct
    18181 gatctgcgat ataaagattc cccgcatcaa gaaagaggac tgagctccga tgcgcgttgc
    18241 cgtaatcggt ggcggaggtt ggggtttggc cctttcccgt ctgctggcga tcaatggcca
    18301 cgaagtcctg gtttgggaac acgactccgc ctggctgcag aggctgcaat ccacccattc
    18361 caatcccgtc ctgctgccca atatcattct gccccagtcg gttaacttca cgggatcatt
    18421 cgccgctata gcgaattttg gctccgacgt catcattctg gccaccccca cccagtttat
    18481 ccgtgccacc ctgagaagta ttaatcctga ggagcggggt ctctggacct cttctgggct
    18541 caaggctatc gtcaacgtgg ccaaggggat agaagagagc agtctatgcc gggtggatga
    18601 gatcattgct caggaattgc cagcctcagc ccggcacctg atttgcgctt tatcgggacc
    18661 cagccatgcc gaagaggtca gcagggaagt acccaccacg gttgtgattg ccggcggaga
    18721 cgaagatacc ctcattatgc tccagaatct cttcagcaac tcttggttca gggtttaccg
    18781 cagcgacgat ctgatcggag tggagatcgg aggggcagtc aaaaacgtga ttgccatcgc
    18841 ggcaggcatc gtggccggtt tgggttttgg ggacaacacg atgggcgctc tgctcacccg
    18901 cgggatagtc gagatcggca ggctgggtaa ggtgttaggc gctaaacccg agacattttt
    18961 ggggctttcc gggatcggtg acctgatcac caccgcgacc agtccccaca gccgcaaccg
    19021 ctttgtaggc tatgagatag gcaaaggccg ccgcctgagg gacattctgg ccgacatgaa
    19081 gatggtggcc gaaggcgtgg cgagcacccg ctctgtgcgt ctcctggccc agaagcacca
    19141 ggtggaaatg ccgatcaccg aacaggtcta tctcactctc tttgaagata aatctcccgc
    19201 tctggctatc cgggacctca tgacccggga gctgaaagag gagtaaacaa ggcgggtaag
    19261 ctcaatccgg tgtctgtaga taatcttgat aaaaccgtca gggccagttt gccgtcccca
    19321 cgaagatgga tgggtgtgcc tcaacccatt gtttatcaaa gtgttatcta agggatggat
    19381 tcccgcctcc gcggaaatga caaaggatag cgggaatcag gcacttaacc cactccctat
    19441 aaaataatgt cttcattgtc atctgccttc cccttgccta ttagcagagg caactatcag
    19501 ctataccatc cccgacccat agctcagaaa tgggctgtgg gtaagctatg ccctggctca
    19561 ttacctgata tactatcaag ggaagatgcc atatccacnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnnnnnnag ctatgccctg gctcattacc tgatatacta tcaagggaag
    19741 atgccatatc cacactgcat tcttcctcat ttgccgccgt ggaaatgtgg cgcgctctcc
    19801 ggcgaaaaac ccgggaggcc gcacggtatc gccacggcag ctaaaaggca aggaagctga
    19861 atcatccaac tcgagcttta tcgatgcacc agggcggaat catgggctaa tgcataggag
    19921 ggtagataat tggagtattc gttactatgt ctggattggc actgaggaga ggcaatggga
    19981 tcttgaccgg gaaaaatatt accgtttcat agattccgct tgacgctttg gaacgtgatg
    20041 gctatttggt tcgaaaacag cgcctgaagt agctaaagat taggatttgg atgcgttacg
    20101 cgcagcagct tcagcaaagt cttcaatctt caccgcccca aatccgccaa ttgattaaca
    20161 ctaaaacgga gtcacagacg agaaaatgga tgccagcgct ctgaaaagag aacgaatcct
    20221 cagcgcggcc atgagtgtgt ttgccagccg gggatatgac aagagctcga tggaagagat
    20281 cgcttcccgg gccagaatcg gcaagacgac cctctattac tattttgaga gcaaagaggc
    20341 gatgtttttg gatgacgttc aggcagctta caacagcttc tttgccgtca tagaggagaa
    20401 acttgccatc cacacccgct ttgaggatcg ctttcgggca atcctgaagc tgccgattcg
    20461 ctttatcttt gaaagcatgc cgatcctcat ggaggctcaa aacagcattc cgcccgcata
    20521 tctggaagtg ctggaaagcc tcaaagccaa aggccgcgac cggatgctgg acctgttgcg
    20581 cgatatcatg gaagcgggga ggcaggaagg tctcctcgat gcaaaactga gcgtggaaga
    20641 cacgatcttt gtggtccatg actggatgct gatgacggaa atgaacctgt ctccgggaga
    20701 caaggacaga atactcaagc gtttggaacg agatcaggac aacctgatcg atatgatctt
    20761 atacggaata atcaaaagag gacaatgatg aaacatgcaa tgctggcagc cggtttctgc
    20821 ctgcttatca gtctggccca cgcgatcggg cttgaagaga gcatcgagat ggcgaagcgc
    20881 aacaaccgcc agcttctgat cgccaaagag gaagtggaaa aagccagaca gacctacaac
    20941 gatgttcgcg gtaatctctt gcctcagttg acgatgcagg gtggatatga gctttcgcgc
    21001 accaatttac cgccttctgc cattcccgct ccggtgagtt tcagtggtgg cttatcggcc
    21061 acggccagtg acgacgatca atatctgggc gcgattctgg ataatgtagt caattccatg
    21121 attccagccg atcccatgga cgaggcttcc gtggccctgc agctcaaaat gacccaggtg
    21181 ctctttctgg gcggaaagct catcaacggc atcaaagccg tggaccgtta tcgcagcatc
    21241 cagcggctaa actacacgct ggaagagcag aagctggtgc tcaataccac tgagctgttt
    21301 tatcaatgct tgctggccgg taagctggca agcgtgcagg aggaaggact cgagctcgcc
    21361 aaccgtcatc tggccagagt ggaatccttt ttttcagaag gcatggtgag cgaatttgat
    21421 ctgcttagag cgcgccttga agtggcgaag ctgcagcctc aggtgttgga agcacaaaac
    21481 aactatcagc ttgtcctggc ggcgtttcgc aggcacttgg gcagtgagga cccggaattg
    21541 aaacccgaag gtgagtttag ccttcctcct gagtgggaaa tcagtctcga ggaggctctc
    21601 agcgcggggc aaaaagaccg ggtcgaactg gagctggcag acatagcgac tgaagtggcc
    21661 aagatcaaat acaatgccga aaagggcaac tacctcccca acgtggcatt gcaggcggac
    21721 tattcactct ataccgcggc ggatgatttt ggcatcgaac gcgatgactt tggctcgcgc
    21781 tacagcgtcg gcatcgggat tcaaattccg ctctttacgg gtttttcaaa tacatccaag
    21841 cgcaaactgg ccaagagctc tcattatcag gccgttctgc aacagcaaga ctacgaggat
    21901 cttatcgctc ttgaaatcaa gcaggactgg caaaactggc atcacgccaa agagaactac
    21961 agagtgcaat cagaaaatat taaaatggct gagcgcagtc tggaacttgc ccaggtacgt
    22021 tacgacaatc aggtgggcat ccagcttgaa gtttttgatg cccggatcac cctttccgga
    22081 atccgtctgc agtatctgca ggcgatctat gaggtgatcg ttaaacagct aaaactgcaa
    22141 aaatctattg gaaccagaat ctaagggagt taccatgagg agatacatag ttttcacgtt
    22201 ggcggtgctt atcgccctga gcttggttgg ctgtggaggt aaaaaggaca aagccaaaaa
    22261 catgcagcag atccaggccg accagggcat ccctgtgcgt gtaagaataa tgggaacaga
    22321 gacatacgtt cagcaattgc gttacaacgc gtccctggct ggcagcgagg aatccaccgc
    22381 gcaggccatg gttggcgacg tcgttaccca aattcatgcc aaggtgggag accgggtttc
    22441 caaagggcag gtggttatca gtttccccat caatactccc gcggcgcaat atgaacaggc
    22501 ctccacagct ttcgcggctc agagacaggc ttatgagcgc atgcagcgtt tgtttgaaaa
    22561 gggcgcggtt tcacgtcagg acctcgacaa tctggaaacc ggttacaagg tcagcaaagc
    22621 caatctggag gccagcgaac agatgatcaa ggtgagagct cccatcagcg gcatcattac
    22681 tgctatccac gtcaatctgg ctgagaaagt ctatcccggc aaggatctgt tcacagtgac
    22741 cgccaccggt ggctataaag ccgtgatgat ggtcccggat actgaagtag caaggattaa
    22801 gaagggcgct caggtttccg ccaccgtcaa cggtgagacc atcaacgggc gtatctctca
    22861 ggtggcgatg gctttggacc ctcaatccag agctgtgcgc gtggaagcgg ttttcccggg
    22921 cgtaaacaaa aacatcagct acggcagcaa cgctcagatc aacgtgaccg tcctttccaa
    22981 accaaactgc atggtggtga accgcgagca catagggttt gaaaatggcg ccgcctgggt
    23041 ttgggtgaat gaaaacaacc gcgcggtccg cagagaaatt cagacggggt tgagcgatca
    23101 gctccatttt gaagtgatta ccggcctcaa cgatggagat caactgatca ccgaaggaaa
    23161 cagcctgctg agcgaaaatg ccctgatccg tctgatcgac cagggggaat agaacatgtt
    23221 tttatcaaag ctctccatcg aacgtcccgt gctggtcacc atggccatcc tggtgttcgt
    23281 ggtgtttggt atgctggcct atatcgacat gccgctcaat ctgatgccgg acgtgcaact
    23341 gccatttgta tcgatccagg tggtatatcc cggagcggga cctgaagagg tggaaaccca
    23401 ggtcaccaaa aagatcgagg acgccatctc gactgtgagc cgcatcgact atgtgcaatc
    23461 ctattctctg gaaagcgtca gcatcgccat catcgccttc gaacttggca aggacgtcga
    23521 tatcgccaat caggaagtca aagacaaggt agacggcatt ctcagagacc ttcccagcac
    23581 ggccgaaaag cctgtggtgc aaaagtttga cttctcttca tttcccttta tggatatagt
    23641 cctcagcgga tctctcgaca gccgggagct ttatgaactg gcggacacca agatcaagga
    23701 ccggtttgcc caaatcgaag gggtagctca ggtcagcatg accggcggcg ccaaacgtca
    23761 gattgacgta aagctatcgg acagctccat ctatgagaac aaaatctcgc tggcccagtt
    23821 gatgcagatt ctggccgccc agaatatgga catgccggcc ggaaacttca accggggatc
    23881 gcaggagtat tccgtgaggc tcaaaggcga gtttggcagc ctggacgaga ttggtgaagc
    23941 tcgtattccc accggtgccg gcatgaaaaa gctctcccag atcgccaaaa tcgaggacag
    24001 cagcgaagag gtccgcaccc gtgccatcta ttttgacgtc tccaggaagt ctctaaagga
    24061 caacatagta cgcatgtctc tgatcaagag tgctgatggc aatgtggtcg atatcgccaa
    24121 acagattgct gcagagctgc ccaaaatcca gaaagagctg cctcagggca ccgatctgca
    24181 gatcatcagg gatgattcgg agtttacaaa atccaccgtc agcgacacgc ttggaaatat
    24241 ctggatgggc atcttgctca ccggtttgat cctgtttctc tttttacacg atctcaggtc
    24301 caccctgatt gtggcgcttt caatgccgat ctcgattatc tccacctttg tgtttttaca
    24361 gctcatgggt ttcacactca acgtgctgac tctgatggga ttatcgacgg ccgtaggtat
    24421 cctggtgacc aactctgtgg tggtgatcga aaacatcttc cgccacaagg atatgggcaa
    24481 cagcagaaga gtggctgccg atatcggaac ggccgagatc gcagtggctg tgatggcatc
    24541 cacgcttacc aatatcgtgg tgttcttacc aatcgcgtcc atgagctcga tggtaggcca
    24601 gtttttcaaa gaattcgctc tcacggttac ttttgccacg ctcgtatctc tggcaacctc
    24661 atttacaatc acgcccatgc tggcctccat gatcattccg gagggcaagc atgacagccg
    24721 ttttggcctg cgtttcgacg ccgcttttgc cagatttgcc ggcctctatc agaagtttct
    24781 cagcgccgta ttgaggtcta aaaaacgcag cctgggcatt attctggcca cgctgctgat
    24841 gctaatcgcc agtttcgcgc tcatgccagt cgtgggcttg gagctgtttc cagccgtcga
    24901 taagggcagt gtaagcatca tggtggaatt gccccagggc tacaatctgc agcagacggc
    24961 caaggtattt gacgaaatcc atcaacggat tggcaagtat aaagaaatcg agcacatagt
    25021 caccaacctc ggaactcagg gcatgatcga caccggtgtc aatctggccg cggcggacct
    25081 caaactggtg gatgcaaaag agcgtaaacg cagctccaac cagatggtgg cagtcctcac
    25141 gaaagagctt gccgacatcc ccaatgccag gatcaaagtg agcatgcctg aaagctttgg
    25201 aggtagagtc gcccccatcg agttttacct ccagggccag gacaacaaca agctggaaga
    25261 gctcaagttg aagtatatca aaacattgtc cgatgttccc ggtctcataa acctggatac
    25321 cagcacccgg gaagggcgtc ccgagatcac catcgttccc aatcgggacc agatggccct
    25381 cgccggtgca actgtctatg atctggccat ggcgttgcgg gccagcgtgg aaggctttgt
    25441 ctccactcaa tacagtgaat ccggcaacca gtatgacatc aagctctccc tcgaagatga
    25501 atacatagac gctccagaca agcttatgaa cctcactgtg cccatctatg ggcaaaacta
    25561 tctgctctct cagcttgcca gagtcgattt tgacagcggg accaaccgga tcacccatcg
    25621 tgaccgctat aagagcatcg tcttcacagg tggcctgact gaaggggcca atctgggaga
    25681 tgtggtctca gaggtgcagg agagagtgga caagatcgat ctgccctccg gctaccaggt
    25741 cgtgtggggt ggaaacgccg aaatgctcaa cgacacggtc gtggatatgg cgcgcacttt
    25801 catcctggcc gtgctgctca cctacatgct gttggccgcc atcctggaaa gcttcgcgca
    25861 gccgctgctg atcatggcaa ccgttccgct cgcgctcatc ggtgtgatcc tttccctctt
    25921 cctcacaggc caggccctca acatcttttc gatgatggca tccatcatgc tggtggggat
    25981 tgtggtcaac aacgccatcc tgatcctgga ctatgtgaat cagcgccgca aagatggatt
    26041 gaatacccac gacgccttgc tggaagcggg agagcacaaa ctcaagccca ttgtgatgtc
    26101 caccctctct atcgtgatcg gcatgctgcc catggccctg gggatcggaa gcaacatgaa
    26161 agagtttcgt cagtccatgg gcgttgtctc catcgggggc ctgatagtct cgtcatttct
    26221 cactttaatc atcattccgg cgttttatta cttgacgaca aagcagagcc agaataaatt
    26281 gtcagaagaa taaatcaagt ggaggaactg atgaaaaagt tcatgaaacc gattcttatg
    26341 gccacactcg ccatcttcct gatggcgagc atgttcggct gatatggcaa ttttgtgctg
    26401 acccagaaac tctacaattg gaacggcacc ctgggcaaca agtatatcaa caactttgcc
    26461 ttctggatcc tttcctgggc tcaggtttac agtgccgcgg tcacgattga ttatgtcttg
    26521 ctgaacaccg tggaattctg gactggcagc aatcccctcg ctatgaagaa cggcgaagag
    26581 aaggtccaat acaccgaagt ggacggttcc aaattcaagc tccgcgtcac caacgacaac
    26641 atcagcatcg aagagatcgc gggtgccaat gtcggcaaga aagttgatgt caaatatgac
    26701 gccgccaccc agagctggta tctggcccag gattccggcg ccgtcaagat cgccacgatg
    26761 aacggcgaca aaatgaacct tctgtatccc agcggcaatc acctcacggt ggacgtcagc
    26821 tactaagctt atctggcggc ggggcttgat gctccgctgc tgtttttaat agaggaacaa
    26881 atgatcgaca aagaaaaact ccaggccgtg ctcgacaaga ttcgccccgc catccaatct
    26941 gacggtggtg atgtggaact ggtaaacgtg cgtgacgacg atgtggtgga agtccgcctc
    27001 acgggagcct gcaaaggctg tcccatggcc actctcaccc tcaaggcggg catcgaaagg
    27061 atcgtcaagg aagagattcc ggaagtcaaa gaagtagtgt ctgtaaagta agatccatta
    27121 ataaatgata tataccgtaa aaggcagcgt cacgctcgcg accagcggga tagtgatggt
    27181 gctgtttacg gtcttttttt tgccgttgag ggcttgggac gcggacctgg gtgtgggaat
    27241 cggcctggcg catgccagct acaggaccga aggcgaattt cccgattttg ccagcaaagt
    27301 cagctacttc cgggatttcc ccaccgtttt tctggaggcg ggggtacccg tctcggaaat
    27361 actcaaactc aacctcagga tctatccaca cagccgcaac tatagcctgg ttgattacga
    27421 tgatgacggc ccccagctca tggagtatta tctcgatatg ccgctttcat tgcgtgccag
    27481 gatcggcagt ctggaagtcg gagcgggagc gggagtaggc tacaaattca ctcatatagg
    27541 catttcagaa attcacagta tagacgatat agaagaatgt gaagatcatc tgcccacctt
    27601 catgccttcc ttcaatgtgg gtgccagagt ccctttgggt aaaggcgagc ccacgagcct
    27661 gaatctcgat tactatcagg atttggggcc gttctcagag gcatggggca ccaaggtgta
    27721 tcacaagcgt tttctggcca ccctgtccta tcgcttcaaa agcatgtctc ccaggcagga
    27781 cttactaccc acccagaaga tcgccgccag cctgggtgat accaatctgg ccatcggagc
    27841 gggatttcat ctggcctcaa tgaaggtgga gcgggttccg gaagaaactt tcgaatcgat
    27901 caatcacaaa cggggcatct ggctgcccgc cctggagatc ggcagaaagg tccatccctg
    27961 gctggaactg cagaccggca tcagcaccaa taaacggcag ttctcaatca aggaatatga
    28021 tgattatgag gacagattta gcctggaagc gaactatgtg gacatccctc tgatcttgcg
    28081 cgccaagctc tatggagtgg agatgggcgc ggggcctact ctctcggtgc tcacagctgc
    28141 caaatacaag cgccgcggag atttggaacc ggaacacgct ccctatgagg ccgccagatt
    28201 gattccagga gccagcctgg ggttcaggta cgcgaagggc agaatggggc tgaatctcta
    28261 ctactgcaaa gatttgacgc ccttctccga gaattatcac attgataagt atcaagaccg
    28321 ctatctatgc tatttatcct accgcctggg ccaggcagaa ccccttaacg atttgctgcc
    28381 cagggaattg gctaggatgc agccctccga atcacatctg tttggaggcc tcaagaaatc
    28441 catctggggt ggcagtggta aatacagtgt ggtgatggga cttgacaaag taagcagatt
    28501 cccttccgga tttggatttg gctacaggct atcagccgcg gctctgagaa gcaagtttga
    28561 tgaaggagaa gcgcttagca tgctcgacct ggctgcccag gcccgtttca gctataagaa
    28621 agggatcggg gaactgtggc tggggccggg atttggaatg ggaagcggtc tagcggtaac
    28681 ggatgctgaa tacttcccca tatttccatt tctggttccc aaggcctgcg tggaagcggg
    28741 tgtcagggtt cacgtgctga gacaggtggc ggttgacctc tcctgggagc agtcctcaat
    28801 cctgtttctg atgtctgggt ttgggctggg atccactaca accagctcat ttggtgtgcc
    28861 gtatctggga ctggcgttag gattttgagg atagattagt ttcccgctga tatgtatttc
    28921 cagcggatta catatttccc gcagatagcg cagattggtg cgcagatatc gcagatnnnn
    28981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagcg gattacatat ttcccgcaga
    29101 tagcgcagat tggtgcgcag atatcgcaga ttttatagag ttataagggt caaacaggtt
    29161 ttatagatta cacagatgct tgatgtaagt ggttgagcag gtcgagagag gttgagatgg
    29221 ttgagaaaga cgtgaagtag gtgtaactaa acccgctaaa ccatccctac ttgtttaaac
    29281 catccccatt acatctgtaa aatctgtgtg ccctcttaca agtctatctg cgatatctga
    29341 gattaaatct gcgcaatcag cgggaaactt ttctgcgaaa tcagcgtgaa aatctgcgta
    29401 atctgcggga aattcgatta cagtgcttcg ttatagtttg catccaggat ctggatgagg
    29461 tcgcgatact tttcccgcag gtctttgaga tgcttgagca gaaagaactc cttttccagt
    29521 gtcaccaatc tggccctgag accgtccttg atatcgtcat aggggatagg agcagcttgt
    29581 ttgtggtccg tgagcatcag aatgtgatat ccaaagctgg agctgaatac cgggctgatt
    29641 tctcccaccc gcatagaaaa tgccacttct tcgattgcag gaatcatctg ccctctgcga
    29701 aagtagccca ggtcaccaca gcgcatgttg ctggggcagt cggagcactc tgaacaggcc
    29761 tggttaaaat catcttcgtt gccgatcttc tgcctgatca gtctggctct ggtttcggca
    29821 ttggcactgc ccttgccgat caggatgtga gaggctctca ccttttcggg atcgctgaac
    29881 aactcaatct ggtcctgata aaaagccatc agctgcgtct catcgatttc cagcgcgttt
    29941 ccgcagatct ggcggatgta tttgcggacc acaatcttgc gggtgatctt gtcttccaga
    30001 gctttagctt cgccgctgct gaggggagag ccgtttccgg agtcatccag cgcttcgagg
    30061 ctctggagca gggccaggtc aaattcatcc tcatcggcct caatgccttg agactgggct
    30121 tcccgataca agagacagct atcgatcagg cgggtgagag tgataatcct ctcccgtctc
    30181 atctgctctg tttctgctcg cggatcaacg agttccgaag ttatctccag ctcggtaatc
    30241 tcataatccg ccactttggc gatcaccaac atctgtgttt cctttttggg aggatcagat
    30301 aaggctcagt acctctctaa tccttgattc taacacagac atattaagct cgtttgatgc
    30361 aattgtcaaa tcgaaatcca gcttgcgata ccatgtgacc tgccttttgg catagttgcg
    30421 gctgtgctgg gctgccagat tgatggcatc ttccagagag catgttcctt caagatatgg
    30481 cttaaactct ttgtatccca agctgttaag ccctggatcg ctccatgtgt agccctgctc
    30541 caacaagctc ttgatctcgt ctatcagccc ggattcaagc atctgggcca tcctgagatt
    30601 tatgcgctta taaagctgtt cgcggggggg catgatcaga atattgaagg ttttataggt
    30661 ttcggagggc tgctgccgtc gccaatgctc gctgagcggg atgccagtcg ccatccagac
    30721 ttccagaccg cggatgattc tctgggggtc atggacgctc accctggcgg caaactcggg
    30781 atcgatcgct ctcagctcgg cgtgcatctc gttgagcagt ttgattctag cttccggagt
    30841 ggcagcgtgg gcgattctgg cgctgaggct cagtttgatg ctcccgggga tgggtggtag
    30901 ctcgcaaatc ccctccagca aagcttttac atagaggcca gttcctccgc aaacgatagg
    30961 tatcaaacct ctggaggcat actcacggat cggaccggcc gcgtccctgg caaagcttcc
    31021 agcgtcgtaa acctctttga tatcaataat atcgataaga tgatggggta cccggcgctg
    31081 ctcttccaga gtgggcttgg cggtgccaat atccagtccc tgatacactt ggcgggagtc
    31141 ggcagatatg ataccggacc caaaagccat tgcaagggct tgggccaggg ctgacttgcc
    31201 tgaggcagtg ggaccctgga tagtgacgag cgggatcata gcaggcgctt gaacttcttg
    31261 tcgaactcct gcatggtcat cttgatgatc agcggccggc catgcggaca gaagaaagga
    31321 gtctgacagg caaagaggtc gttgatgagc gccagcattt ctctgcgggt gagaggctta
    31381 cctgctttga tagcggcctt gcaggcgatc gatttggcca aagaatctct aaaatcctga
    31441 ttctcttcca gttcggattc cagttgtttg aggatgtcga taaagatctt gccaccctgc
    31501 cagtcggaaa gctccgcggg gatttcttcc accaccagcg acgaaccgga gaatttcttg
    31561 agcacaaagc ctgtcttctc aagcagttcc atattctgat ccaccaactc gcggatatcg
    31621 gtagcgatat atggagggat gtccaccacc agcggaacga ttagtttttg acggatggga
    31681 ggagcgccgt gggccctctg gatcagcttt tcatagatga cgcgttcgtg ggccgcgtgc
    31741 tggtcgatga tcacaaggcc atcttccacc tgaacaaaga tatagctgtt gtggagctgc
    31801 caggggttga tgtagtcttc ctcgttcttg agcaggatac gatactgcgg tgggggagtg
    31861 gactctttta tggatccgga aacgtccccg cttccctctg tgacctctcg tggggagccg
    31921 taagcgtccc cgcttgcctc tgagacctca tcaccatctc tcgcggaacc ggaaaccttc
    31981 ccgtttgccg catcggaatc caaggccaca tccttgcgcg gaggtggagc aaagaggtca
    32041 tcctgccaga gctcgccaaa ctccttcttg tagctggaat aacggggcac ttccacccgg
    32101 ctgcgataga tgctttgttc cagagtcgag gcttccgaaa ccgcggcggc ctgatgaaac
    32161 ttgtctctgg cgctggcgaa aatggcgttc tcataatccg tgagggcatc cgtgagtgtg
    32221 ccgtgcacca gagcgtggag tttctgctgc tccctgaagc gcacctccag cttggcggga
    32281 tggacgttta cgtcgatctg ttctggtggg accgagataa acagcatata gggcggcgtg
    32341 tatcccttgg cccagacacg ggttttgagg atgaagggtt cgtaagcgct ccggatggca
    32401 tgacggaggg tcttatcgct gatgtatctg ccgtttacga aagtatattg gatgtcgatg
    32461 agcttgtcgc gccgctcttc cagaccgaag atgtagcctt ccacaccata gccttcccgc
    32521 tctgtctgca cagggatcac atcgtcctca aagaagccac tgccaaacac ttccgccatc
    32581 cgctgacggc ggtcccgggt gccgatatag ttcaggaggg tcttgccctc cgagatgagc
    32641 cgaaaactga tggatggcca cagaatggat tgataatgaa agtatttgag gatgtgccgc
    32701 agctccgtct gagcgctttt gagaaacttt ctacgggcgg gcagggcttt aaaaagcccc
    32761 tttacggtga tgttggtgcc gggattggaa gccgtctgat caacattacg gagtctgccg
    32821 ttggaaaaat cgacgcgggt ggcggtttga tctgctgttt ggcgggtgat gaggctgagc
    32881 tcgctcaccg aggcgatgct gggtaaagcc tctcctctaa agcccaggga gccgatatgg
    32941 atgatgtctt ccactgttct gatcttgctg gtggcatgac gttcaaaggc cagcatggcg
    33001 tcgtcacggc tcatgccatg tccgtcgtcg gatacctgga tcagatctct gccgcccgct
    33061 tccacggcaa cggtgatcat cgtggctcct gcatccaaag cattctccac cagttcttta
    33121 actacagaag agggacgctc gatcacctcc ccggcggcga tcttgttgcg gacgtcttca
    33181 gaaaggactt ttatccggtt cacttttacc ccttcactct tcactcttca cgaaactcag
    33241 taccggaaga tcgagccatt tgtaaactca cggatgatgg agttataggg gatcagcgcg
    33301 tccgggatat ccctgatatg agaaagcaga tgcagcaagc ggcgtgattc catatacgcg
    33361 gagctgtttt tggagacgcc ctgcagcagt ttccaggcgt gtttgcgcag gatacccagc
    33421 tcgtaggtga ggctgctgtt aaacatatag tccgcatttt cctgataggg gaagatgttt
    33481 ttctcttcgc cttcgcgcac gtccacccat ctctgcaagg tttcgatggc ggaatagcct
    33541 ctgtattgat ggtctctcac gatccttctc agcagccggc agtcggtggt ggggatgcgg
    33601 ttgtggttgt ctatgttcaa ctgattcaaa gcgctcacgt atatttttgc cttgctggag
    33661 gctggcaccg aggccgtgag cagatcgttg agcccgtgga tgccttccat caccagcacg
    33721 tcgtctttgc ccagcttcac aaagttgttg ctgcgtttgc gcaggcctct ggtgaagtca
    33781 tacataggca gctcaacttc tttgccggca aggatatcgg tgagctgctg gttgagaaat
    33841 tcgaggtcca tggcatagat ggactcaaag tcaaaatcgc cgttttcctt gcgggcggtg
    33901 cggtcgcgat cgatgaaata atcgtccatc cccagcacga tgggtttctt tttagaggcc
    33961 tgcagctgca cagacagccg tttggcgaaa gtggtcttgc cggaagagga gggtcccgcg
    34021 atcaggatca gtttcacttt cggcttcttg acgatgctgg aggcaatttc ggcgatcctc
    34081 ttctcgtgca gcgcttcctc caccaggatg aactgcgaga tgtcatagtt gtctaccagc
    34141 ttgttcaagt ccgtgatatt gtgcacgttg aggatatcca gccacttatc gtgttcctga
    34201 tgcagggcaa agatcttctg cggcagcttg aagggtttgg caatcgtgag gtctttaccg
    34261 gaggggaatc tgaggataaa tcccggggcc tgatagacga tgtcatactc gtcgatcatg
    34321 ccggtgcggt ctgccagcgg acggatgtag aaatcgtagt atctgccgca gcgataaatg
    34381 gatacgctgt cccggtaggt ataacggaga ttcttctgga ggtccttgcg gcccatattc
    34441 ataaagatat ccaaggcctc cgagagcttg acttcctcac ggtcgatcgg cagatcggct
    34501 tccacaatca cccgcatttc ctgtttgatc cgggcgcagt cctgctccgt aaacttgtcg
    34561 gtgccaaaca cttcacaaaa cacgccgtct cccacgctgt gttcgatcac cagcgagtgc
    34621 ttgtctccca ataggttata caaagccttt ccgaggatga agattgcgct gtcctgatag
    34681 atgcgaaagc cttccggatg ccggatggtg atgcagttga ccatcgtgtt ctgctgcagc
    34741 acatattcgt cattgacata ttgggtatgg tttattttgt aggacaacac gctatgcgga
    34801 tttatgtccg tttcgcggat cacagccgcg atcggcttgg ggttttccag ctcgatcatg
    34861 ctgtgctgga tgccgttaag tctgatgtct atgttcatta gtcttttcct gtagattttt
    34921 tcagcgtcgg gatccgtttc gacggatgtc tgacgaacgc gccataattc tcagcaagcg
    34981 ataaccgtca agtgaaagga gatcaagtga ccaagctcac caaagcgcaa cgcctcagaa
    35041 gggaaatcac ccatctggcg gggattatct tcggagcagt gttttttgcc ataggctatt
    35101 cctggtttct cttgccctac aacatcgctc ccggcggcgt gggaggtatt tcccaaatcc
    35161 tgtctttcta caccgggatc cccaacggcg taggcatgat cctcatcaac ctccccctct
    35221 ttgtgatcag ctttatcttc atcggtcggt cctttggggc caagtctctg tatggcatgc
    35281 tggtaactgc tgccatgacc gatctcatga gctatccgat gctccacaaa ctgggtttga
    35341 tagcggacct ggcaaaatat acccacatcg taaacggacg caccgtctat gccatgctgg
    35401 gaccagaaga tatgatgctg accgcgatcg cgggatcggt gatgctgggg ctgggactgg
    35461 ggatcatttt ccgcttcaga ggctccaccg gcggcaccga tatccccgtg gcccttatca
    35521 agcaaaaagc cggaatctct attggcagtg gctattacat cgtggaaact ggcatcattc
    35581 tggccgtggc gatcttcctg cgtgatccca aaatcctcat ctggggctat atcaacctct
    35641 ttatcaccac caaaatcacc gacctcgcct cggaaggcct gccatacatc aagggggcct
    35701 acattatctc ccaggaagtg gacgaaatca aggaagaaat ctataaaaaa ctggaccgcg
    35761 gcgtcacctt cttcaaaggc ctcggcggct atgacaaaaa agagatcgac gtgctcttct
    35821 gcgtgctcaa ccgaaggcag gtaccgctgc tcaccgatat cgtcaaagac tgcgatccgg
    35881 acgcgtttat gatcctcaca gacgtctatg atgttttggg atatggattt aagtctcgca
    35941 agatagatct cagcgacggg taggtggact taggattggc gggtttgatg gcatgctggt
    36001 tggcatgtct tggagtgaaa tcacccctcc aggagttcac agccttggcg ggatagcctc
    36061 cagaggcaga agcagagtga taaaagtccg gagctcatga ggcgcgaaag cttccggggc
    36121 caggggtcaa tctaaagcca ggatctgatg cgcttcagcg cttcctcatc gcccaggatg
    36181 gcaaaggtgc tggggagatc gggaccgtgg gcctgaccga ttaaagcgag gcgcagaggg
    36241 gtgtagaatg cttttccttt cagtccggtt tcggcgatcc cacgtttgac cagctcctcc
    36301 actttttcag ggtttagagg catggccgcg gggatttggg tggcaaacca ggacagcacc
    36361 agttgagcac tatcagagcg aacgctttgc aggtcttctc cttccaaagc attgggggag
    36421 ataaacatcc ggcaataaga ggggatatct gaaagcaggc tgcagcgttc cctggccgct
    36481 ccaaccatgc gcttcagcgt ctctttttcc aggatgggca gattttgttc cacaaagaat
    36541 ggcagcgagc gttccacgac agtctcgagg ggcaagtcgc gcagatattg tccgttcatc
    36601 cagtccagtt tggtgaggtc aaacaccgcc ccggctttgt ttacgcgctc caggctgaag
    36661 atttcgcaca gctccgcgag gctgtatagc tctctgtcgt cggcaggatg ccatcccagc
    36721 agcgcgataa aattgagcag ggcctctttg agatagccct tttgcagata gctttccacg
    36781 gagacgtcgt tcatgcgctt gctgagcttg ctgcggtctg gattgagaat gaggggcaag
    36841 tgcacccact gcggggcttg ccagtccagg cattcataga gccagatgtg tttgggggta
    36901 ctggaaagcc attcctcgcc tctgatcacg tgggagatcc gcatgtagtg atcatccaca
    36961 acggcggcaa gatgataggt gggaaagccg tccgatttca ggagcacttg atcgtcggat
    37021 tgggaggcat ccatctccac gtgtccgcgg atcaggtcgt caaagccaaa cttgcgctca
    37081 tccggcatgc ggagcctgag cacgtgtttt tctcccgcgg ccagtttcgt ttccacttcc
    37141 tgccgggaga gcttcagaca gcggcggtcg tatttaacga actccttgct ggcttgttga
    37201 ttagcccgca tttcttcaag tgtttcctga gagcagaaac agtaataggc atggccgctt
    37261 tccagtagcc tctgtgcttc acggtgatac agctccagcc gctcggattg ggtatagggg
    37321 ccataatcac ctcccaagcc aggaccttcg tcgtaatcga ggcccagttt gcgcagcgag
    37381 ccgatgaggt tttccaccgc tccttccacg aagcgggatc tgtcggtatc ctcgatcctg
    37441 agcacgcagg tccccttgag tgagcgggca aaaagatagt catagagggc ggtgcggagt
    37501 ccgccgatgt gcagccatcc ggtgggggag ggggcgaaac gtactctgat ctcttccaaa
    37561 acgtagttcc agctgctagc gatcgatgtc gaaggcttgt ttgagcacct tgtgatgcaa
    37621 gatgcggtaa tggggctggc agtagctttt gaaggggctg gagtgatgtg gaatagaggc
    37681 ttccaggacg tgcgcctgca cctcttttac caattctgga gagggcttat agcgcgcttc
    37741 aatgcgaggc cacagcctgc gccaggtggc tttccaggag cggacgtcga tctccatttc
    37801 cagacaggag tcaaggatca tctgagctac tctggcaacc tgagcgtcgt ctggaacctg
    37861 cttgatcagt tttttgggca ggctggaaac gggaatgggt tcttcgcccc aagccggaaa
    37921 gaatggtatc aggtcaagtg aaatgcgata aaagccttta cggtttccaa tatcttgcag
    37981 cagaggctga tagagcttgc ggttctttgc aagctcctgt tccaggctta tttgaatcac
    38041 ttcgagatcc ttgcccatat ggctggggcc gtaataagac tggcagagca gtttataggc
    38101 gtctagcagg tttccctgag gatggagcct ggcttccagg tccatgatcg cgttgagatc
    38161 gctggcgtta agagctatgc ggcgagtaag catgtactga ctttcctttt ttgactatgt
    38221 atttaacctg gacgtcacga tagtggaagg cagccgtgag atcggtgtca agcacgctga
    38281 ggtcggcctg aaaaccaggt gaaagcttgc ccagacggtg ttcatcgtga ctcagccagg
    38341 cggcgttggc ggtatagatg cggatcgcct ccacgggagt gaggcgctcg gaaggattgc
    38401 ggtgatggat acaggcatag acggacatgg cgacgttcat tggcgtcaca taccaatccg
    38461 aggagcccgt cactttcacg ccctgtgcgg taaaactggc aaagcggttc atctgcgctg
    38521 cccttgcggt tccaagacgg agggcataaa agccgttttc gcctccccag aggagatcaa
    38581 agacgggctg catgacaggc accgctccgg aggctttgat ctgggccaca aggtcatccg
    38641 gggttagctc gcaatggatg agctggtgcc tgagatcggc aggatgtttc tgctgaagct
    38701 caaggtagat ggtgttgatt tgtcggatcg ccgcgtcccc gatacagtgg acgcagacct
    38761 gcatgccgtg ctggtgggcg gatgtgataa actctctcca aaagcgatcg tcgtgataga
    38821 gcgaaccgcg ggtgtcggag tcctcgtagc tggtgctcag ggcagcggta tggcttccga
    38881 tggaaccgtc cgccaggatg cagcctccga tgcgttttgc accgactgcc atggccgcct
    38941 tgatgttaaa gctttgggga tagaggatat actcgatggg aagctcagag cttctggcct
    39001 gcagaaactg aaagtgatca atgctctgat cggcgtctcc aatcatggtg tgaacggtgc
    39061 agaagccgcc tttcatcgcc actttggcag cgctgtgata agccttcagg atgccggtct
    39121 catcgcaatt ggagtgaaac caatgggtgg cgcggtcgtt atcttctcct cttagaatga
    39181 catcgctgga tttgatcgct ccgatcagcg ctctggagtg ggagctcatc atacagctgt
    39241 gaccgtctat cctccgcaaa agtaaatggc tgtctccggc tgcctggtcc agttcctcct
    39301 gagtggggaa gcgtttttcc gccagcgaat tctcatcaaa gttccaggcg aagagcaatg
    39361 caccgggctc caaatcacgc gcggcccccc cgatcctgtt gatgagggag ctaagatcct
    39421 ggcagtcttg cagatcgatg cctccgctgt agagccctcc ggaaaaactg tgggtgtggg
    39481 tgtcgataaa accgggatag gcataatcgc cgcccagatc gagatcctca ggacccaaac
    39541 cagccggcag agtgctcagc agttctacaa tgatgccgtt ttccaccctg agggctttga
    39601 cgtcgttggc aaaactatcg tcgagataga acctggcgtt gtggatgatc atgccagcag
    39661 ctcctgacaa agctgataaa agcggtggat gggaaagccc attacattga aatagcagcc
    39721 tttaatgccg gtgataaact gagctccata gccctggata ccataggcgc cggctttgtc
    39781 catcggctcc tgggtttcca cataaagcct gatctcttct tcgctcaagg ctttaaaggt
    39841 gacgctgctc ttttcccagg cttgcaggca acgcccctga tacacaaggc agattccggt
    39901 ataaacctga tgcgaccgcc ccgacagcag cctcagatac tggctggcgc gatctatcga
    39961 atccggcttg cccaaaagat gccgatcgag aacgacaagc gtgtccgccc ccaccaccaa
    40021 acaatgggaa tcgacccgcg ccgctacagc tcttgctttg ttgcgggcat gacgcagtgc
    40081 ctggacccgg ggcgcttctc ccgtatccgg ttcatcagcg tgggaaggca ccacaagggc
    40141 tttgacaccc atcattttca tgaggtcccg gcgcctcggg gaggccgatg ccagcacgac
    40201 tttcgtgttc tctaataact tgtgtatcat ataaataacc tgcgaacaca caaatcctga
    40261 ggggggattt acgtcaacat atttttagac tctcaaaaaa tctcttgcct gaaaacaagg
    40321 cttcggaaaa gcttgtctca aaacacaaaa atacctatcc aggaggataa tatggaacgt
    40381 gtacacaact tcaacgccgg tccggcagtc ttgccggaag aggtgatgct ggaagcgcag
    40441 gccgatttct tcaactacaa aggcatgggc ctttcggtga tggaaatgag ccaccgtagc
    40501 aaggagtatc aagccattat cgacgaaacc tacgctgccg tcaaacgcat ctacggcctc
    40561 ggtgacgact atgaagtgct gttcctgcaa ggcggtgcca gcatgcagtt tctgatggtg
    40621 ccactcaact ttgccttcga cggcaaggaa gccaacttca tcaataccgg cgtgtggtcc
    40681 aaaaaagcca taggtgaagc caagaagatc ggcaaaaccg tccacgtcgc cgccaccagc
    40741 gaagaccgta acttcagcta tatcccgaag agctggcagc tttcggacaa tcccagctac
    40801 ctgcacatca ccaccaacaa caccatcttc ggcaccgagt ggaagaccga cccgcaggtc
    40861 ccagccggga ttccgttgat cgccgacatg tcctccaact tcatgagcaa gccgatcgac
    40921 gcgcagcgct attccatgat ttatgccggc gcgcagaaaa acgtcggacc ttccggcact
    40981 gccgtcgtga tcatcaaaaa ggagctcctc gaaaaggcca ttccaggcca gccttccatg
    41041 ctggattatc agttgcatgc caagaacggc tccatgtaca acactcccgc caccttcact
    41101 atctacatga tcggcctggt gctgaaatgg attgagaact tcggcggcct tgccatgatc
    41161 gagcaaaaca acattgccaa agccaaatac atctatgatg ccatcgatgc ctccaacggc
    41221 ttctataaag gcaccgtgga tgtcgcggac cgctctctga tgaacatcac cttccgcatg
    41281 cccactgagg agttggaagc tcagttcatc tctgaagcca agaaaaacgg catgatcggt
    41341 ctgaagggcc atcgcgacgt aggtggctgc cgggccagca cttataactc attacccctg
    41401 cccgccgcgc acgcgctggg tgaattcatg cgcgctttcc agcagcagca tggttgatca
    41461 cacaatctga gggccggtgg agtatctctc tgccagccta gtttatcgtc acggcgggcg
    41521 gtagctgtcc ttaccgctcg ccttctcaaa ttcacaagtg taaaccagga aactatatga
    41581 cagtcttcag tctgtttctg cggggcggca ttctcatgta tgtgctgttg ctgctttccg
    41641 tggccgtgat cgccatcgtg atagaaaagt atcgcctcct gagatcggtg cgcaattcca
    41701 accgcaagat ccagtcttcg ctgcatcaac aggataagct ggacaacgtg cgtgccatcc
    41761 tgagagtcta cggcagcaat tctccgctgg gggccatgct caacaagctt tacaactcca
    41821 actccaaaga catcgccctc atcaaagaaa gcatggaatc caccgccaat catgagcttc
    41881 acaagctgga acgcggtatg ggatggctgt ctacgtttgc cgctatcgct cccctcatcg
    41941 gctttttggg caccgtgatc ggaatggtga gagtgtttat gaacattcag ggccagagcc
    42001 agaacggtat cgacatcaac atcctggcag gcgggatctg ggaagctctg ttgaccacca
    42061 tcggcggtct ggtggtgggc atcgtggcta tcatccttta caacgacctg gtggtaaatc
    42121 tggagaacat tgccaaggaa atgcaggaac aagccattga atacgtgatc cgctatcaga
    42181 aaacggaaga gtagatcatg aaaatcacaa cgtccagcaa aaaaatcagc accaccgtgc
    42241 tgatctccat gacggatgtt atcttcctgc tcatcatctt tttgctgcta tcctccaatt
    42301 tcgcgtcgca gaccggattg ccgatcaagc tgccgtcctc cacctcctca cagcgccaag
    42361 cccagcaggt gctgcacgtg atctatgagc acgacgcctc catcctgctg ctggacaagc
    42421 gttacactct gatgaatctg gagccggcgc tgaaggagaa gtttgtgagc aaggaccagg
    42481 tggtgaggct ttcggcggaa aagcagaccg aactgcagaa gatcatcacg ctcatggacg
    42541 tgatccgcaa cgccggattt gaaaggattt tcgtggctac cgagcgcccg gaaccaggta
    42601 atgccagata gataccgtca atacctgcct ctggccattt cagccgggct gcatcttgtc
    42661 ctgctgatca tcacgatgct ctactacctg cctctgatga agcgggaacc ctggtatgaa
    42721 gtcagttggg gcgaacccgt ggttgaagag cagatagtgg aagaggtttc agaaccggcg
    42781 gcagaacaag ccagcctgcc gatgcagtct acccctgtga aaccggttgt gaggcaagct
    42841 ccgccatccg tgaggcccag cgcgccactc aatcccccca aagcagaaga tatcggccct
    42901 ccccgtctgt cagaaagcga agtggtggaa gctccaatat acacccccac agagccagcg
    42961 atcaaggccc cgccttccct gagaagcaat ccccaggtca cggacgcgct gaggcaaacg
    43021 gtgtccggta tccccgccaa caacagcccc ggctccgtgg aatcagacct cgaggggggc
    43081 aaactgatcc gcacggacac caaacaccgc actcactctt ttggcgacta cggggaagta
    43141 aaactgagtt tcagcgtcga tatgtccgca cggttgattg caagctccat ccgcgtaatt
    43201 caatcccaaa acagcaagtt tgacgccgag gccatctccg tcctcaaaga tatgaccttc
    43261 agtttccggg gtcgtcccga tcccgaaaag ccctatcaga tcacgatcaa attcagcccc
    43321 tgattatgag cgccaatcct ctgtcccagt tcaagatgcc cgctttaatg gggattctga
    43381 acctcactcc cgattctttt tcggacggag caatgttcaa cgatcctgag cgggcgttgg
    43441 aacacgctcg cagcctgctg gaagcgggag ccgggatcat tgatctggga ggtgaatcca
    43501 cccggccggg agcgctggaa gtatctgtgg aagaggagct tcaacgggtg ctgcccgttc
    43561 tcagaaagtt gggcggactt catccccgcc ccagaatttc catcgatacc cgtcacgcca
    43621 ggaccgctga agcctgtatc gcggagggtg ccaatctcat caacgacatc tccgccttgc
    43681 gtcacgatcc cgatatggcg cctctgctgg ccggctttcc ccaggtggat gtcatcctga
    43741 tgcacatgcg gggccagcct gaaacgatgc aagtggctcc ccggtatgac gatctgtata
    43801 acgatataaa gggttttttt atagagcgga tcgagttttg cgaggcccat gggattgcca
    43861 gagaacgcct gtttctggat cctggaatcg gcttcggaaa aacggcggaa cacaatctgg
    43921 ccctgctggg ggctttgcac agattcgcgg agctgggctt gccgatcgtg ttgggagcat
    43981 cccgcaaacg ttttattgac gcgatttctg cctccgcgcc ggatcaacgt ctggccggca
    44041 gtctggcagc cgctctggtg ggtgccatgc agggagtggc tatcctcagg gtgcacgacg
    44101 tgtttgctca caaccaattt ttcaaagttc tcagagcaat cgtgggggaa aaagcttgaa
    44161 ttggctgatt ccgcagtttc gcgatatggt ggacatcgtc ataatcgctt tcctgatcta
    44221 ccaggcgctc agcatcgtgc gcaagagcgg tggatatcag ctgctgaccg gcattctgct
    44281 gctttttcta ttgtatctgg cctcggtgct ggtaaatctc aagatgctct ccgcggtgct
    44341 cggcgcggtg cgcaattact ggatcctggc gctgatcatc ctctttcagc cggaaatccg
    44401 gtctatgctg gcccgcttca acctctccca ggatatggga atcttcaaaa aacgcgaaaa
    44461 caccaatgtc tttaccacgc tggtggatgc cgtgtcttcc atgtctttcc gcaagacggg
    44521 cgctctgatc gtggtggaga ataaacgcaa gcttaacgag tatatccaaa acggcgaacc
    44581 gatagacgcc agcatctctc tgcgcctcat cctcactatc ttcaatcccc gctccagcct
    44641 gcatgacggg gctataatca tcagagggga ccgcatcctt gccgccaagg tcgtcctgcc
    44701 gcttaccaaa aagagcgaat atgtaaaacg ctttggcacc aggcacttag cagggatagg
    44761 tatttcagaa ttgaccgacg cgattgccat catcgtttcc gagcaaaatg gccatgtttc
    44821 catcgcccag ggtggcgtga tccaaaacaa cgtcgccttt gaggaattga tgcagatcgt
    44881 gtcggacgcg gctggataag ccatggccgc tcaatcactt ccacgtccta taatcatcgg
    44941 catcacgggc aatatcggca gcggcaagag cagtttttgc gcctttttgg aacaagccgg
    45001 cctcgcagtt tattatgccg acctgctcgc gaaagccgta ttgcgcgacc ccgaagtctt
    45061 atcaaagctg gcagccagat ggggaaacga gatcgcggag gatggcaaac cgcgcagcga
    45121 agtgattgcc gctaaagtct ttggcaagcc ggaagagctg gcctttctga attcccttgt
    45181 ccatcccggc actctgaaac agatgcaaca gcttgtggat caatcaacta ctcctgtcgt
    45241 gctgtttgaa gtccctctct tgtttgaagc caggctggaa gactgttttg actatctcgt
    45301 cctgattacg gccccgcttg aactgaggac gaggcggatc atcgacaggg acaaggctac
    45361 caggcaacag gtcggtcagc gcgccgccgc tcagcttccg gacagcgaga aggcatcccg
    45421 ctgtgatctg gtgatcctta acgacaaggg tttgaaggac ttagaagagc aggccctgat
    45481 ctttgtcagg cagcttcctg agttgcgtac ccgccccaag cgtccctttt attccggcaa
    45541 ctgactcacc cacaatcgcg tcctgaacca tggtacctgt gcccctctct atctccccca
    45601 acccatcccg aaccagattg gggatgggta cggggagcct caagcgggga agagaggaag
    45661 ggctaagggc ggaagcgctg acttagcgga ccgcgtttct ggccagtcgc atcccgatat
    45721 agttgtagct caagtaatcc gtgctgaggt cgcggcgata gccgatgcgg ctttcttcca
    45781 gcgcgtcaca ccaggcactg cctctttgag cgtgcacact gccctcagcg ggacccttgg
    45841 gatcgatgct gccgttttcg gactggtaat ccaggctgtg ccaatcccag caaagctccg
    45901 aaacgttgcc agccatgtca tggattccca ggaagttggg cgagaaactg ccgacgggtg
    45961 aggagaaagc atagccgtcc tcgtaaccct ccatgtagcc ccaagggaag tcgtcaaatt
    46021 gttcatagta ttccagatgg ctttcatcgg caaggttggc aggtggttct aacagagggt
    46081 tttccagcag gtcatcgtcc ccccaggaat ataaatagcc catgcctctg tcgctggcag
    46141 cgaactccca ttccgtggca gtgggcagcc tgtatccatc cgcctcaggg ttccaggtga
    46201 cggtccagcg cttgatatcc acctcgtttt ggttgttggg gtctttgttt agtttgtcga
    46261 tggtgtaggc aggggtcagg ccggattccg cggaaagcca gttgcagtaa gccaccgcgt
    46321 cataccaggt gaggctggcg acggggtgat cgtctgcctg ttcgtatcca ggatcacgcc
    46381 agctgaggcc tgctttctct gtccaggcgg cttccaccag tccaaaaccc gctcctttga
    46441 tttccgcggt gctgagatag ccggtggctg tgacgaattc tctgaattct ttgagggtga
    46501 gctcatacct ggcgatctgg aagtcgctga cccgcacttg gataggttga tcgtcagcac
    46561 ccaggccgac tgctatccat cctcccacga cggggaccat atcaggtcct gccgccagca
    46621 taggcaccag cgcgagcagg aacaacaaaa ttaagtttat ctttctcata atagactcca
    46681 tgtttagtat cattgctgat aatatacatg gcccgcggct ttgggaaagg agaatttcag
    46741 cctaaacagc aggaattcgg gtggccagcc aaaaggccag caccagaaga ccaattatcc
    46801 acatacagac agagattatg cccaggattc tgccatactc ggtgagatcg actccacttg
    46861 agtccatgac gcccatgttc attttacgca ggtcaaggtt gccatagtgc caggccagac
    46921 cgccgaatat tcccacggtg aaaaagccca ggatacccag cagcaacacc agccggcctc
    46981 tgtgaggctg cagtgaaaga tccggggtgg gctcaaaggg gtcgtagtag ttcttgtgca
    47041 actgagggat cagtcaatac ccgggaagcc ctgttacagg ctccccggca gtgaatggtt
    47101 tacttgagca ccagaccggg gatcaatccg ccgatcagga agcaagtggc aaacgcgata
    47161 atcgcgtaaa gctctggcag cacacccagg atcagggtgt tggtaaaaac gttgttaccg
    47221 tttccgaggc tttcaatacc ggtggagacg atctttgcct gttggatggc agagatcagg
    47281 cccaccaggc ccataatcag acccgccgcg aagacggcag aggcctgcaa caggttcata
    47341 ctgtagctga tgtgggtctg caggataaag aaagctccaa atccatagag cccctgagtg
    47401 cctggcatag cgctcaggat gaggcaatat gggaagatat cgtcacgttt tttcatggca
    47461 ccgacgctgg cgctgcctcc cataactgtt cccatggcgc tgccggttcc ggccagggcg
    47521 accatcagga aaattccgat ccaagcaagg atgtagggca tgtaacctcc caggttatat
    47581 taatttattg tttattccca aagggtttgt attggatgcc ggggccttgg aaatccgcgt
    47641 ttttaaagaa ctccacaaag gtgagacgca tgggatgcac aaagcctgaa agagccccca
    47701 gagcaatgtt cagagcatgg cccaggagca tgaaaacgaa aaagatgacg ggcccgatga
    47761 tgggaatccc cagcatctga cttccgatcg aattgatcac gaagcccagg atggcgctcg
    47821 atactcccag cgcgaacagc cggatgtagg agaggatatc gccaaagaag cctgtgatga
    47881 cgttgtagag cagccacagg ccgcccagca cgttgataat ggggtttttg cccggctggt
    47941 tgaagagcag cagcagagcc aaacccgcaa acatgggaaa cctgacatag gccttgactg
    48001 ccgaaatatc cgtacccagc atttcggctc ccaacacgga gagcgaaccc aggaagagga
    48061 agatgccgat gggagcgacg ctgtgcataa agccactctg acggcctttc ttgacggcgt
    48121 tgatggccac tccaaagagg atctggatcg ctccgaggag cagggcaaag ttgaagatct
    48181 ggtcgttgtt gcgaatgatg atggcggggt ccagcaccgt cttcttgaga tcaaaggcca
    48241 gggcggagcc catcagccag cccatcagca tggaggagat gccaaagatc acgatcagca
    48301 tcatcacgct tttggcagcg gggtttttgg ctttgtgacg caggaacagg cccaaggcga
    48361 tgaaaaccag gccgtaggct atgtccgcgt tgcaaaaccc gaagaagagc ataaagaagg
    48421 gagcgaagaa gggcgtgagg tcaaactcgt tgtaatatgg caacatatac atattgccga
    48481 taggctcgaa cagccgtgaa aaccagttgt tacggagctg gataggcggg ttgtcgtccg
    48541 ctttcccgtc ctgagcgaag tggatgatgc ccttgtcgtt aagataggtg gagagctcgc
    48601 gttccttgct cacggggatc cagccgccca gcactctgag gtggtcctct gcttccgagc
    48661 tggcttgcag ggtggcatcc tcataatcat attcgccgct gatccgctgg atctcttcct
    48721 caaagcgctg aatcgcttcg ggagcggtct cgttgaggaa caggtcgata tccgcgattc
    48781 tctgttgcag ctcctccaat tgggtttcaa actcccggag cgtgtgtttg tgaaagctga
    48841 aggtatcgca atccagggct ggtttctcac cggtgtacaa gaccacaaag tagacgatcc
    48901 cgccgagctc gttgatctgc cggatgcaat actgctccag ccattcgggc ttaaagtggt
    48961 ttttgagaca ggtgtgaaaa tccactttaa cacccgcgtt ttcgagctgg gtctctaatt
    49021 cgtaatcaaa atggccccag ggacttagct ctctgatctg ctttttgagg atgtcttcct
    49081 gacggcgcag gtcatccttt tcggagacgg cctgctcgat cctgttgagg agtgctttgg
    49141 tgggcagact ggtgttgcct ttttccttgt ccgagccgat cttcttgagc agtttgaggg
    49201 cgtcgttgta gcgtcccatc agctccaact gccgtacctg ggaatcggtg cgggcatcgg
    49261 tagagcggat aatatgcacc acgcccagcc gctgcagatc ggccaggaag ctctcataat
    49321 cgctgtggaa gagcacaaag ctgtattttc tcatcttttc gatcatgcgt tcgcctccgc
    49381 tgccatgcgg cctttgagaa tcttctgcgc ggctttggag agattctctt cgtcttccat
    49441 aaaacgggtg atcttgagga tggcctcttc gtaagccggg atctgcacct tttcgtagag
    49501 gttgaccttc tgcgtggtct tcttgcgcac tacctccagc agctgcatct tgcgcaccat
    49561 gaactcccgc tctatctgca tgcgggagag ctctttgagg gtgctgacgc catccagcca
    49621 ccagctgggg gccgtgaaca ggtcatactc gctcaactga tattcaatgc cttccagctc
    49681 aggggttttg acgcccgcga tcttgcggga gcggatttta acctccgaga tcttgatcag
    49741 cgagggatca aactccaccc agagcttgag gaattcttcc agcctggcgc cgcgggcttc
    49801 gatctccagg tccatggcgt gagcggcatc gcgggctttt ttgacctcga aacgcaaggc
    49861 cgattccttg tttttgagcg tcggcagagc gttttccctg attttcagct gcttgcgcat
    49921 attgagctga gagatcttgt tatactgaaa cttgagggtc atgccttctt ccagtagaga
    49981 ttcataaatt catcttttat cgccacttcc tcgcgggtaa agtacttctg aaacagctcc
    50041 caggtggtgt ccagcatctg gtccgtgtcg atattgacgt cgatggcgag gatttgcatg
    50101 gcataatcat aggcgaattt gagggtgcgg tcatcatagt ccgagagctc gaagccgttt
    50161 tccatcttgg ttttggcttt ggtggcatcg gcgttgaggc gcaccgaggc ttccatcact
    50221 ttgggatggt cggcacgggt ctttttgccc atcactttgg tcttgagacg cgaaagcgag
    50281 cgagccggat cgatcaccac cttgccgata tcggtgtcgc gccgcaggta gaggttgccc
    50341 tctgtgatgt aaccggtgtt gtctgggatg gcgtgagtga tatcgccgcc ggagatggtg
    50401 gtcacggcaa tgatggtgat ggaaccgccg gtggggaact gcacggcttt ctcatagatt
    50461 ttggcaaggt cgctgtacag tgatcccggc atggagtcct tggaggggat ctggtccatg
    50521 cggttgctca cgatcgaaag cgcgtcgcag taaagcgtga ggtcggtgag cagcaccatc
    50581 actttttcgt tcttctcgat ggcaaaatac tccgccgcgg ccaaagccat gttgggcacc
    50641 agcagtcgtt ccacaggcgg gtcgtccgtg gtgttgacga aggagatgat cttatcgatc
    50701 atcccggcgt tttcaaaggc gtttttataa tagagatagt cgtcgttgga gaggcccatt
    50761 ccacccagga tgatcttatc cgcgggggtt ctaagcgcca cggtggccat cacctggttg
    50821 taaggctggt ccggatcggc aaagaagggg atcttctgtc cggtgacgag ggtgttgttg
    50881 aggtcgatac cggcaatccc tgtagcaatc agctccgagg gctcttcacg ccgcaccgga
    50941 ttgacgctcg gaccgccgat ttccacttcc ctgccttcga tctgagggcc gccgtcgatg
    51001 ggatcgccat aggcattgaa aaagcggccg gagagctcgt cgctcacttt gagggtgggc
    51061 ggtttgccaa agaacagcac ttccgcgtcc gttccgatgc cttccgtgcc ggagaatatc
    51121 tgcagggtca cgttgtcgtc gatgatcttc accacttgcg caaggcggcc gctgacggtg
    51181 gccagctctt catagccgac tccggtggct ttcacggtgc aggtggcctt ggtgatctgg
    51241 gtgagcttgg tgtaaacctt ctggaaagcg gtggtggcca ttatttcgcc ctcctttcgt
    51301 ccatagtttc ttgcagctgg gcctcatagc ctttgaaagc ggagctgtga aactcgctgt
    51361 agttcatctg tttgaggatg ttgatcagct tggtgtaata aggctgcacc tcttcatagc
    51421 tgtcaaacac aaaatcggtg cggcataccc ccaaaaccat ctccagcata tagctctgac
    51481 gcttcatggg agtggatttg tccaccacgt caaagccgtc ctgctgcagg atgatgcggt
    51541 ccagcagctc gctcttccac aacctttcgt gataggccat ggggacaccg tcgtcaccca
    51601 gaatattgat ctggtcgccg gcttccttgc ctctcaggag gatgtctttt gcttcataaa
    51661 cgtctgccac ccaatgagaa gtaatgtttt tgttcaagta attctgcacc tcgtcatact
    51721 caagatattt ggaataggac tctatagggt ccacagccgg gtatcttttg gagtctgccc
    51781 tatcctggga gagcgcatag aagcagcggg ccgctttctt ggtggcttca gtgaccggtt
    51841 ccttgaggtt tccacccgcg ggagacacag ccccgataaa ggtgatggag ccgggaacgc
    51901 cgttgttgag gtacacataa ccatttctgg aatagaagtt tgagatgatg gcgggaaggt
    51961 ccatgggata ggcatccggg ccgggaagct cttccatgcg gttgctcatt tcacgcaaag
    52021 cctgggccca gcgggaggtc gaatccgcca gcaggaggat tttcaggccc atgctgcgat
    52081 agtattccgc gatggacata gccgtataga cagaggcttc gcgggccgcc accggcatgt
    52141 tggaggtgtt gcagatgatg atggtgcgtt ccatcagctt gcgtcccgtg cgggggtcgt
    52201 ccagcttggg aaagtccaca aagagctcca ccacttcgtt ggcgcgttca ccacaggcgg
    52261 ccacgatgat gaggtccgct tcggcgtttt gggagatact gtgctgcagc acggtcttac
    52321 cggtaccgaa aggaccggga gtgaagccgg tgccgccttc cgcgatgggg ttgagggtat
    52381 cgatggtgcg cacgccagtc tccatcatct tgaaggggcg cggcttctct ttataagccc
    52441 ggatcggcag cttcaccggc cagtattgca ccatgttgat ctcaaaggtt ttaccttccg
    52501 tatccgcaag ggtggcgatg gtgtccgtga gcttgtaagt gcctttctcc gctatgcttt
    52561 ggagcgtgta tttatcttcc atcgtgaagg ggaccatgat cttgtgggat atccagtttt
    52621 caggcacttc acccagccag tcaccagcgg agaggctgtc tcccggtttc gcgatcggcg
    52681 tgaattccca actgctgtcc tcatcgaggg gatcggtata ctcaccgcgg ttgaggaaca
    52741 tgccttccat cttttcgagg tcgtcctgaa gtccgtcata gatcttggag agcatgccag
    52801 gtcccagctt cacttccagc atcctttggg tgaattccac cgggtagccg ggcctgagac
    52861 cgcgggtgct ttcaaacacc tgcgtgtagg cgatatttcc ttggatctta atgacttccg
    52921 ccttcagttt cacgccatcc atgtctatat agcagatctc gttctgggag acgggtccat
    52981 ccacctcaac gtgaaccaga ttggcgataa ttcctttgac gatacctttg gtcattatcg
    53041 ttaccttcta tttgtttttt tgtttgatcg cgaattcggg agggaaggag aagctgtttt
    53101 gcagagtgtt cagcgtatcg taaaagactt ccttgccgag gtttacatcc atcctcatcc
    53161 agcgggagag gatcagtagc tgagcaaaat agcccagcac tctgtctatc gaaaagtatt
    53221 ggaaaaagtt atggctgtcg atccatttcc agctcaggat atcataggcc cgttcccggt
    53281 agagcactga gttttgctcg taagctctga ggtgggcatc gaacacaggg ctgcttttgc
    53341 cgatgccaaa atccactccg cggccgtggg caagcttatc gttgagctcg tcgtcgccga
    53401 tcagccaacg ggcgtaatcc agctcgtgag cgcggccgtt gatggccagg actacgttgc
    53461 gggactgtct gcgatactcg aaccaggctg aaacaaagcg gttggaatga gccgcggcaa
    53521 agctgaacag gtcctgataa aagccgtgtt ccaccatgat tctggccggc ggttcttcca
    53581 ggctgagaaa ttccacgcag cgcttcagca aaaagggagg caggtctttg aactgctttg
    53641 gcaccggcgc ttcgcggttt tccaccctca ggcgggcaaa ctccccaaac tcgctccaga
    53701 aggccgcttc atagtagccg ggacggtcag ggggcgcttc ctgtttgaag agcaaggcgc
    53761 tgagggtttt gatcccatct tccagagtga gcagcttcag gagcttgaga tcgtcgtccg
    53821 tgagctgagt ggcggcttgt tccagaaagg cttcaggctc gatctggagc ttggcgtcgt
    53881 cgatgccgat cctgggaagg ccggcaacaa aatagtaata ctgaccggag ctcatcgcgt
    53941 tattcggaat agagcaattg ggcggtgcgg ggcctcaggt agctcttgaa caggttggca
    54001 aagtcttcat ccgtgaagct gagcttataa cctccgtccg cgggggccag ggtaaacccg
    54061 tttttgaccg ccgggctgta gtccaccttc agaccgttct tcaaggcttc agccagagat
    54121 ccctgcaggg cgtcatccag cttgccgcgc atcgtttccg cgatcgtgat gctgccaccg
    54181 ctgcttttcc acgcttccag agtgctgagg atgatctgtt tgagaaaggc gggatccgag
    54241 aaatgggcct tgatgggatc gtccagcacc ttctgaagga ccacctcggt gaggcgttgt
    54301 tttacgctgc tgatcgtgtg ttgagcggcc atcttgagat cggtttcgga gtttttggca
    54361 agctcggtgg attgtccccg ggcttctgag atgatcttgt cggcctgcgc ctgggctttg
    54421 gaaatcgtat cgtcggcagt ctgctgagcg gcctgcaata tcttgtccgc ttcggcttta
    54481 gctttggcga cgccctcctc atgcacacgc tgtaaaaggt cttgcaactg gtctgtcata
    54541 gctttactcc agcttgattt tcactttgat ccatctttac aaggctgccg ttttatgtca
    54601 acaacagagt ttggtttttt tgctataaga gggagggagg gggtttttag gttttagggt
    54661 ttcagggttt tactgc
//
LOCUS       DAOSFN010000003        41343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_731_length_41343_cov_8.766518, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000003 DAOSFN010000000
VERSION     DAOSFN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 41343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41343
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_41343_cov_8.766518"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1191)
                     /locus_tag="PLO35_00535"
     CDS             complement(223..1191)
                     /locus_tag="PLO35_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOH78176.1"
                     /translation="MNIKAGIVQELKTEADAILKMAARLDEQAVETAFELLSGCEGKV
                     VLTGVGKTGIIARKISATLASTGTASIFLHAAEGIHGDLGMIQSSDVVIAISNSGSGT
                     ELISIIPFLKFIGTPLIAFTGNMNSTLAEAADVALDCSVPQEYEPLGMVPTSSTTVAL
                     ALGDAFAIALLKSRNFSLNDFARFHPGGSIGKKLILKVSDLMHSGPELPVVEGSASMK
                     DAILEMTSGKLGCTAVTDEKEILLGILTDGDLRRQLQIHPDGLLNLPVRECMTPNPKR
                     LRPTDLAVSALRLMEDHSITMLPVVDDQDKVLGMLHMHDLVRAGVV"
     gene            complement(1208..3112)
                     /gene="thrS"
                     /locus_tag="PLO35_00540"
     CDS             complement(1208..3112)
                     /gene="thrS"
                     /locus_tag="PLO35_00540"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOH78177.1"
                     /translation="MNIKITLPDGSQKEYQQPVSALQVANDISPRLADAAICAEVDGK
                     LRDLDHLIEQDADLILHTFKTSAGKEIYWHSTAHLMAQAVKQLFPGVKVTIGPAIEQG
                     FYYDFDRDEPFTDEDLVKIEERMKELSKSDLHYQRRELSKAEAMEKFAEMGEDYKLEI
                     LSEIPDTDTISTYQQGDFIDLCRGPHISSTGKIKAVKLLKTSGAYWRGDEKNKMLKRI
                     YGISFPSNKELAEYLDFLEQAALRDHRKLGKDLDLFSINDEVGAGLVLWHPNGAMIRH
                     LIEAYWKDEHLRRGYDLVYTPHMGRSNLWQTSGHLGFYKENMYSAMEVEDQQYYIKPM
                     NCPFHISIYNAGHHSYRELPIRLAELGTVYRYERSGVLHGLMRVRGFTQDDAHIICRP
                     DQLDEEVEKLIIFSLDMLRHFGFQEFLIYLSTKPESSVGEQGDWDKATQSLANSLAKL
                     DLKYDVDEGGGAFYGPKIDIKIKDAIGRAWQCTTIQFDFNLPQRFDMQYIGSDNTAHR
                     PYMIHRAVLGSVERFFATLLEYHGGNLPLWLCPKQIKIIPITDAQFDYAHKLSEKFAL
                     LGFRCEVDNRNEKIGYKIREAEMKKIPYMCVIGKKEEESGALTLRRHTEGDLGVMSFD
                     EAVKLIREKG"
     assembly_gap    3390..3489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3595..5676)
                     /locus_tag="PLO35_00545"
     CDS             complement(3595..5676)
                     /locus_tag="PLO35_00545"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOH78178.1"
                     /translation="MRTREYDIHNLDCAHCGNKIESVVNELPGVTNAQLDFVNKRLIV
                     SYNEDIENVIEKLNYLANSIEPGVSITVKGDAVEEKSRDLRFWLPLLLGVMLLIISYG
                     AGLSGSMSVALGLISWLLVGHRVLISAVRSIGKGQVFSEQFLMSVATAGAMFLAEYTE
                     AAAVMVLYEIGQWLESLAVAKSRSAIKGMLAIKPDKAHKLTEEGTVDCSLDKIEIADV
                     LLIYPGERVPLDGEVRKGNSALDTSTLTGEAAPLAVGAGDRVLAGTLNLDGLIEMEVS
                     SLDAESTVSRVLKLIEEASTRKSPSERFITQFARYYTPAVVAAALLVFLVPTLLGWPA
                     SVWLKRALVFLIVSCPCALVISVPLSYYVGIGKAARKGIVFKGGVYLDVLRKVKTMVF
                     DKTGTLTTGELKIDKLVTPEDGSPELLIDTIYRCEYTSNHPFAKAVRQAYTADYDSAM
                     VNAYSEYPGKGVLLLYGEERLIAGSPVFMKEQGFIDLIDSDGMSAVHAVKNDIYLGCM
                     SFTDELRPGMKAALSDLRGRGVNKMIMLSGDREAKAQALSEELGLDAWAAELLPHQKI
                     EQLEATMAGSTGLTAYVGEGMNDAPALARADLGIAMGQIGNPASIETADVVLLQDRPA
                     QLVEAFKQAHETHRTVSQNIILALGIKTLVMVLGVSGISGLWEAIIADVGVTLLAVLN
                     ATRHGINISRR"
     gene            complement(5673..6032)
                     /locus_tag="PLO35_00550"
     CDS             complement(5673..6032)
                     /locus_tag="PLO35_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH78179.1"
                     /translation="MITPCLCKMIDDKEIEQARRELPDAGTIERLRDFYQVFADGTRL
                     KILYFLTRHELCVADLATLVGMGQSAVSHQLKVLRMNRLVKVRKDGTTYYYSLDDEHV
                     RSIFEVALEHVHEGSSR"
     gene            6187..7143
                     /gene="trxB"
                     /locus_tag="PLO35_00555"
     CDS             6187..7143
                     /gene="trxB"
                     /locus_tag="PLO35_00555"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOH78180.1"
                     /translation="MRYDVIIIGAGPAGLSAAIYSARGGLKTAVFERAFIGGQITVTD
                     EVENYPGFEDALSGFDLTERMRAQAARFGAEFHDEEITAMGMEGLCKIIETGKDKYRA
                     KAVIICTGAHPRRLNVPGEERFTGRGVSYCATCDGALYRDKVVAVVGGGDSAIEEGIF
                     LTRFAKKVVVIHRRDQLRAQQIIQQRAFKNPKMEFIWDSVVQEIQGEAMVEKLEIYNR
                     KTNEISYIPADGVFIYVGILPNNELLESRIELDSAGFVLTDEFMHTNVPGIYAAGDIR
                     HTVLRQVVTATSDGAIAAWSAEKWIIENYDEIEGMESNHSAE"
     gene            7133..>9654
                     /gene="alaS"
                     /locus_tag="PLO35_00560"
     CDS             7133..>9654
                     /gene="alaS"
                     /locus_tag="PLO35_00560"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOH78181.1"
                     /translation="MPSSRDIRQAFIDFFRERGHQFVPSSPVVPQDDPTLLFANAGMN
                     QFKDIFLGLRDAEHPRAANSQKCIRAGGKHNDLEEVGTDGYHHTFFEMLGNWSFGDYY
                     KQEAISWAWELLTGVWGLPKDRLYATVHHTDQEAYELWKTQTDIEPSHISYHGDKDNF
                     WEMGETGPCGPCSEIHIDRGEGHCDKSHIPGHVCDVNAGCARYIELWNLVFIQYKREA
                     DRSLSPLKNRFVDTGAGLERITQVLQDKRSNYETDLFMPIIERVAELSGQTYDPQKGV
                     SHRVVADHIRCLCFALADGGFPSNEGRGYVLRRILRRAARHGRLLGFAEPFLHQLVDS
                     VIAVMGHHYTELNGKQEYIKTIIKAEEERFNHTLDTGLERFAEIKSRLEGDTIDGKDA
                     FMLYDTYGFPLDLTVILATEQGLKVDLEGFEAEMTHQRQRARSASRFSLQTEDIEWIV
                     LNELSPTQFVGYEQHQCDCHIQRYHISEDGKIHLQLDRTPFYAESGGQIADTGELLSE
                     QFQVRIQDVRKQGDEFIHSGRIISGAPHPQIMHARIDEARRQEIARNHTATHLIHRAL
                     RDILGEHVQQKGSLVAPEYARFDFTHLKALSRDEIDKLEQQVNDVILQNRQVRTDLLP
                     IEAARQQGAIALFGEKYGDEVRVVSVQDFSKELCGGTHVSATGEIGLFKVLSESSSAA
                     GIRRLEIVTGAHALHYVNSWQERLHSMAGKLKTSDKLLEDKLEALIAKNRELEEELKK
                     QSAKQGGEQVNELLTQAENLNGFRFLAVEIQAETAEQLRGLGDALRDKAPDLLAFLFA
                     PQSDKVSILCVAGPKLGNRFHAGKLVGSVAAFLDGKGGGRPDS"
     assembly_gap    9655..9754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9755..10449
                     /locus_tag="PLO35_00565"
     CDS             <9755..10449
                     /locus_tag="PLO35_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOH78182.1"
                     /translation="RYDKQRSLRQAIVAHRSDQSINSTVQLYYSALDRLTGIAPDSFK
                     PPQLRLPDISLTPERMPKGIPGKRLIGIFPGAAHLTKMYPEQQWREFFQIADADWSFV
                     LLGSLKESRIADRLMTALPDRIENRCGSYSFEELALVMSQCDLIISADSGPMHLAAAL
                     NLPQIAIFGSTHPRLGFAPQNPKAIVLCADLPCQPCTLHGQDYCPLGHFECMKKISPG
                     MLKDKVVKVLGV"
     gene            complement(10564..12570)
                     /gene="uvrB"
                     /locus_tag="PLO35_00570"
     CDS             complement(10564..12570)
                     /gene="uvrB"
                     /locus_tag="PLO35_00570"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOH78183.1"
                     /translation="MSIFKLASEYQPSGDQPQAIQELVSGIREARPHQVLLGVTGSGK
                     TYTIANVIASFDRPVLVLSHNKTLAAQLYGEFKQLFPENAVEYFISYYDYYQPEAYIP
                     GKDIYIEKDSDINEQIEKLRLRATMSLMERRDVIIVASVSCIYGLGVPEEYREALIRL
                     DVGARMERDELIQKLVLAHYSRNDIAFERGAFRVRGDIVDIYPAYLEHCLRVEFYGDE
                     IVALQKLHPISYQVLESVESYPVYPAIHFIMNEDKMRGALQSIEREMEERVKYYLDNQ
                     RYVEADRLKQRTMFDLEMLRELGYCSGIENYSRHLTGTPAGSPPSCLLDYFPEDFIFI
                     IDESHVTIPQVHGMYGGDFTRKKNLVEYGFRLPSAFDNRPLRFEEFEHYLRHVIFVSA
                     TPANYELELTKGVVVEQVIRPTGLVDPEVEIKPIKHQVDDLIAQVKERVGKGHKALVM
                     TLTKRMSEDLSTYLNNAGIRSKWLHSEIDSIERSKIIRGLRLGEFDVIVGVNLLREGL
                     DLPEVSLVAILDADKTGFLRSARSLIQISGRAARNVEGKVVLYADEITDAIAQTLEET
                     NRRRVKQLAYNAEHGITPQTIQKSIEQIMQSTSIAEGYASIDADKDDETKPSKADFSE
                     YLEIDNRDKLIALLKKEMQRAAAKLDFERAAELRDRIAEMETGI"
     gene            12744..13064
                     /locus_tag="PLO35_00575"
     CDS             12744..13064
                     /locus_tag="PLO35_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78184.1"
                     /translation="MVNLNDLQINFDNLLKLGYAVLDIRFKEYDLCPDSHKLVIARVD
                     SDRDEFYQDMLKQYTAQEFKANEVSEIWTDILKHKVKMSSVLNRDIAIKVAALDYIET
                     VYNA"
     gene            13065..13475
                     /locus_tag="PLO35_00580"
     CDS             13065..13475
                     /locus_tag="PLO35_00580"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOH78185.1"
                     /translation="MTDRSLLLIKPNAVQHHHVGHIISIVEDHGFKLRDIKIFQFTSA
                     SAEEFYEAHRGKEFFERLVRFMCSGETIALLLERNDAVELLRELVGDADPAKRAPHTI
                     RASYAEGVTENAVHASDSIAAAKREIKLIFGANY"
     gene            complement(13692..14183)
                     /locus_tag="PLO35_00585"
     CDS             complement(13692..14183)
                     /locus_tag="PLO35_00585"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOH78186.1"
                     /translation="MSPARPDIEAPNRVELDILLQSTDEYPSSAFVVCDFDVYAVFGT
                     RARVPVIVKVGEHQFRSSLAPMSGSHMMVFNREMRDATGFKAGDRVHMILERDVQDRQ
                     VDVPEDVKAAIEAEGKWEAFITQSYTHKKEQMLWINEAKRPETRQRRIEKLLRDHLSK
                     RLK"
     gene            complement(14180..16558)
                     /locus_tag="PLO35_00590"
     CDS             complement(14180..16558)
                     /locus_tag="PLO35_00590"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOH78187.1"
                     /translation="MELIPILWYLAPLGATLALAFALVFFYQVKAQNPGTPRMIEIAG
                     YVREGAFAYLKQQYKGVIIFFIGAFIVFQVMALGLGVLHWLVPWAFLTGGFFSGLAGF
                     IGMNMATLASNRTAQACSESLNQGLKVAFRAGSVMGLTVVGLALIDISAWFFILNHFQ
                     VPLQKITVIMLSFGMGASTQALFARLGGGIFTKAADVGADLVGKVEAGIPEDDARNPA
                     TIADNVGDNVGDVAGMGADLYESYAGSILATAALGMGAVAMLGESFKVWQLNFVIAPM
                     ILAGIGAILSIVGIFMVRTKEGATTHDLMFALNKSVYSSSALIAVASFFVTRYLLPPD
                     YYFGVFLSSVIGLAIGILIGWFTERDTSHSYRPTQSIAEQGKFGPATVILEGISVGMR
                     STAAPVITVAVGIMLAFICSHGFQSIEMGLYGIGFGAVGMLATLGVTLAMDAFGPIAD
                     NAGGNAQMSHMHDNVRELTDNLDSVGNTTAATGKGFAIGSAALTAMALLAAYLEEVRA
                     GLLLLGDKVGKTLFMNIHYSRLSSDVVSVEHASIQDFINYYQISALNPKFLMGIFIGA
                     MVTYLFSALTIKAVGRAAGKMVDEVRRQFTTIPGILEGTSKPDYKSCVKISTVAAQHE
                     MVLPAMVGIGTPVVVGMLLGVAGVLGVLVGALAAGFVLAIMMNNAGGAWDNAKKYVET
                     GKHGGKNSGAHKATIVGDTVGDPFKDTAGPCINILIKLMSMVSIVFAGLIVSYSLQVE
                     TIRPPLSKVKPDKAMRNEIVGISQNSPCSGCPALSSGCVSDSSQVLKGEKTP"
     gene            16842..17804
                     /locus_tag="PLO35_00595"
     CDS             16842..17804
                     /locus_tag="PLO35_00595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH78188.1"
                     /translation="MGSFTAELYDHLVPITANNFISLANSGFYNELIFHRVVHGFVIQ
                     DGCPYGTGYGGPGYTIPDEFHPDLNHNEAGILAMARTSAPNSAGSQYYFTLAPAPHLN
                     GSYAVFGKCIQGLDVIMAIGNVPVDANNRPLTPVTIDTLRILDMAITNLTPANDETIE
                     CSANEEQFFIVEASSQILPITYRWFVDGAEQPGFSDFIFGYSFGIGSHTVSCGMTNAD
                     WAHTVTWDLNVQATYNSDPEVPSPTYLTLSPNPFKEGTRVDWKSPSNDDLSIYDLKGR
                     LIRKISGASGSFVWDGSDSRGNRCPAGIYLFKAGERTEKGLLLK"
     gene            complement(17809..18963)
                     /locus_tag="PLO35_00600"
     CDS             complement(17809..18963)
                     /locus_tag="PLO35_00600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH78189.1"
                     /translation="MIKILNIITRLNIGGASIHVVEISQMFQGGVYSSTVLYGDVESN
                     ESDMLYLAKEYDFPMINLPTMGRSINLLQDLLLIPKVYKIIRRLSPDIVHTHTAKAGL
                     VGRVAARLAGVPVILHTFHGNNFRGYFGKLMSLVSINLERALARISTRIIAISDQQRD
                     ELLSFNICQAEKLRVIHLGFEFGKIIHDDSHQGMFKAALGIPVETKLVGFVGRLTAIK
                     NPFSFIDIARRVLKERDDVIFAFVGDGELSESLKAEVRLLGLEAKIIFTGFIKDLRPL
                     YADMDALLLCSLNEGTPVAIIEAMANRVPVLASKVGGVPNLIKDGQSGWLFAHQDKDG
                     FASCLMKVLEDPELCDRITAQAWQDISDRYSAQRLKAELEQLFQELGVKP"
     gene            complement(18960..20045)
                     /locus_tag="PLO35_00605"
     CDS             complement(18960..20045)
                     /locus_tag="PLO35_00605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOH78190.1"
                     /translation="MSEARNYLKIMVMPLVWLLWKIRQAFTRFSNLEPKSICVLYLSG
                     MGDILCDSFFFRELRARYPQAVLTACFPAPFCDLQRNYFQFDKYIAHQDYWTTLKELW
                     KLNCDLIIIPGWLTRNSILAILSNARAIIGYVNDLSFTNRYLNSFTLEAAGIRVNRFR
                     QDMRLCHLAQRPAAISAALGIPHLEVTSLSIPRLKEARNYAVFHAGARFPGRRWKEDN
                     FCKVAEWLLQSAYVDTVYLIGDEADKAINLRIRAFSKSKAIIDKAGGMSLVESFELIS
                     AARFFLGNDSGPMHISALAGVPTLGLLGPNFPHISGPLGTQSRWIFHEFPCSGCNQRT
                     CDYGYRCINAITVEETLQAIKQMTGIS"
     gene            complement(20038..21300)
                     /locus_tag="PLO35_00610"
     CDS             complement(20038..21300)
                     /locus_tag="PLO35_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78191.1"
                     /translation="MKDKRILMLINEFPPVGESGVQRPLKFLKYLARSGWDCLVVTPS
                     QPTKTVLDKSLCREIPQSARIIRTPSWGLRGKSVDLVASIRQKSVSHSIKGLSSRLLM
                     RLNHMLFPIDKQIGWVPFAFIYALFFIKRYKLRNLYITAFPFSAMLVGILLKKCLGDK
                     LFWVADYRDGWQFEPLIESVLPKRRMRFIRWMDEQVLRHCDKAVFVTESIRCQYIKAY
                     PWMSKRAELITNGYDEDDFTDLVRQSFETPTLVYMGKIYNLNRPNLLPLLDALKGSDL
                     STPLIHIGSLALDARAQIERNGYDFYNFKGYKTHREALSIAAGAAVNLLLLNDDKVSE
                     GVYTGKVFELLRLGPPILALGPKQGLVKDLIETSGAGVYVWVQDSEAIIPALKRLLEH
                     PESYRCRPDILQNFRRSYLTERLAALYE"
     gene            complement(21290..22459)
                     /locus_tag="PLO35_00615"
     CDS             complement(21290..22459)
                     /locus_tag="PLO35_00615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOH78192.1"
                     /translation="MFMKPKKLLLITDMFPHEQSPVAGIFVRHQAEALARHYQVKVVA
                     TWFPSQPELRITQTPEFELVYIRYPQSRLLFPLTMLQYRKWVIPRLKALISSWGPDII
                     HVHDCRHVPELTCLADVLKSCSAAKYLSVHNIKTLPERAEYAYLRYIYRKTLAQAYDH
                     WDKVFFVNEGLRQRMKEIVPLEKSMFIGNAIIPAQPCTDLFIKELAASLDKEDFNILA
                     VANLKREKGLDILIRSLAEIRYQGKRVSLKIIGDGEERERLLDLALRLGVERQVTIYP
                     AKANQVIRQTYKYFDLFALPSYSETFGIVYLEALDAGIPIIGVKGQGIDGVIASGEHG
                     LLCEPRNQASLSDAIRYMMEHQEEAKAMAEKGQELVRGSFMMDSLIAKLREVYEG"
     gene            22680..23708
                     /locus_tag="PLO35_00620"
     CDS             22680..23708
                     /locus_tag="PLO35_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOH78193.1"
                     /translation="MDKIRLGLIGCGRISKNHFDAVSQIPEADFVAVADVIEEKAKTM
                     AASYGITSVYKDYREMLEKEQLDAVSICTPSGMHPQIGIDVANSGRAVITEKPMGISI
                     EQADKLIQACDANNVHLFVVKQNRLNSTLQLLKRAVEKERFGRIYIAQSNVFWQRPQA
                     YYDAEKWRGTWEFDGGAFMNQASHYVDAIYWLLGNVDSVMAYTATMARRIEAEDTGCA
                     ILQFRSGIIATLNVTMLTYPKNFEGSITIIGEKGTVKVGGMAVNKIEKWEFEDYDDDD
                     RIAQDANYQPPNVYGFGHNPYYRNVIDVLLGRDTPSTDGRDGRKSVEIIQAIYRSAKT
                     GKRVSLPL"
     gene            23924..25198
                     /locus_tag="PLO35_00625"
     CDS             23924..25198
                     /locus_tag="PLO35_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH78194.1"
                     /translation="MRRFIIILIALAVLFGGCGKAIQSNKVKVTFWHGLSGPLGDTLA
                     EMIREFNRTHKDIEVLANPISSYAALSQKLMASIQAKKQPDIAQVFESWTAKYVAADV
                     LVNISDLIKEDPGFTEADFEDMYPVFRNAVTYQGNIWSFPFNKSVRAYFYNKDAFYRA
                     GLDPNHFPQTWQEFRDYCKTFTKDAKPRKIYGTNYGPNEWQFINLLHQAGGRIIDDNG
                     QPVLNSPAGVEALTYVTDLINKDKTVYTVKEYDGQNDFLAGIVAMYEGSSVSITHMRQ
                     QPINFNMGYAPLPTYRTAQSAISGANIVIFKSGDSKREKAAWEFIKWFTDTAQTAKWS
                     VKTCYMPVRRSAMETKVVKDFLAETPQFQGIFDQLEQAVYEPQVPAWFNARTELKGFL
                     EKAFKQVLTPKEALDQAAAKFTELIAAEEVQK"
     gene            25247..28471
                     /locus_tag="PLO35_00630"
     CDS             25247..28471
                     /locus_tag="PLO35_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78195.1"
                     /translation="MGLFDRALSSLRLRLNAAIIGKALIATLLIFLLGLHLYFIAWIG
                     FSPQSPLLPYINITLRIGMALIALFILITAYNRLLSHYQVARWMDLSADHGDDLYQNL
                     LQLRQQGESQEVLEVLALGAQSRLGEHKYPPPPWYKPTHFFLILFFLVGMISVWGLSY
                     DSFRLALKQFYSNKAESIDYKRTVEVSPGNLTIGKGQELIISVLNPDPRLEHRLFFRY
                     DKAWRQLAMTDFRYRFEALEYDIEYYVANEVAASDTFRIKCLDEPFVKRWELRYQFPP
                     HTSLAARTDTLSLGNIEAYRHTLVSLNLVTNIPVKTAVMSFADGNKIDLQGAGNSFST
                     QIRINQPGTWYLELVDELGRRNRPEEKTISIIPDAPPEVRIVFPGEDTLLDQRMNLPL
                     IISANDDFGLRDLSLRFNINDTPTRELMIRSVMGGKIHNQDYLFDLKPFELFPGDRVT
                     YWVQVWDNSPDRQKAESARYVARFPSIAEIYQEIERQESLKTSELERTLEDTRDLQKE
                     FEQKRRELLKEPDPSWEDKKQLEDILQKQEQLADQVDNVAEDFQELIDQMRNNQALSP
                     ETLQKMQKIQELMEEISNDELRAAMEKFNDALGKMDQEALRKVMEDFKFSMEDFAQRI
                     DQTLQLLESIKKEQAVEKALQMSQEIEKSQAALKERSGDTKQNPESLARDQENIASRY
                     EELKKELEKLSDMMDPARDQEVTQQLEQLKKDMQQSKPEQDMKSSAQQMRQNQRQDAQ
                     QNQSSALEKMRRFTMKLAEMKKSMGGGSQKEVMEAMQRAIRELLIFSKNHEALKGRLG
                     TDPYPIMQELIAQYDGLQILLNKFFSTPQVTMFIPPKFYLDLSDTNRAYREVFVNVSD
                     MQYYRLPELMSGIQKGLNLMVYDLMQALNNPSQGGGGGGMQSLMQMLEQMGQEQMAMM
                     MLTEQMMMQLQQQGGRMDAAMQQQMQKLASDQQRLADNLKRALQNDPEAQKQGNAIKQ
                     IIEEAEAVARQLRSNQLSNDLMRRQENIISRLLDAQRSINKRDTTQKRKGETSTQQFP
                     QKAGEIDLKELRRAAMLDDSYKSLPAAYQQVIIKYLKYLNDKAQ"
     gene            28468..30201
                     /locus_tag="PLO35_00635"
     CDS             28468..30201
                     /locus_tag="PLO35_00635"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78196.1"
                     /translation="MTRYMRICLINFILIIIVSGLWGQYDEKQILTQQAQNFYNSRQY
                     AQAETIYKQVLERWPGDQLAILQLLNIAYQTGNIDKAEAILNEHGRYLQPATVLDHEL
                     QILVQRGSVDAAWEKALRSLELNPKDQNRYRIIASYFERRGFYDQVLRLYELGRHQFN
                     NPELFSLEYANSALNYQRYELALREYLRWLDRNPGNLYFINNQCKLVLEADSSLVYVI
                     EEAAGTGSNPVMKELLAGTLVSRKRYNDALNVYKEMGQEKLLLFATEQFNAMNDEVAQ
                     PAFAWLAGREADIWRKSQYLYNLAVIGTRNQRYTDADSLVTAVINDSLLSLPQNRWRN
                     PVSYQARKLKAELSLILSRDPQEALNWIAQAKSFARNAPEIQECELEAARLLILDQDY
                     EAASQKLALVTEARLIEKRDHLSFLRALLSGDVALADSLMNEYVITWPEGQFTNDAIY
                     LMMHALALSDEARAHFMAAYRANQLRDPFAPTMLITLFAGTQDEELLILAVEWALAMG
                     DKAQALSILSHDWQDEVSKDYAALLSLFLQESGEEQQRQAREFLRQNPNSVFSPSFRT
                     RLIRESRGKPE"
     gene            complement(30409..32082)
                     /locus_tag="PLO35_00640"
     CDS             complement(30409..32082)
                     /locus_tag="PLO35_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOH78197.1"
                     /translation="MAFLPINRQDMASRHWGCLDIILVSGDAYVDHPSFGIPLIGRVL
                     EAAGYRVGIIAQPDLDSDADLLRLGAPRLFFGVSAGNMDSLINHYTAQRKRRNDDAFS
                     PSGLPEKRPDRASIVYTNQLKRLFKNVPVVLGGLEASMRRLAHYDYWQDKVRSSILAD
                     SKADILVYGMGERAILEIASALGQGSSVDSLKDIPGTLVFASKTEIPEPGTLLPSDRE
                     CVDKRTFWKASRLFWSEHDRQTLYQVNAGRLVRHNPPATALNSAELDRIYAMPFERAP
                     HPIYQNKLIPAWEQIKTSITSHRGCYGGCNFCAIAAHQGREVSSRSKASIRAEAVIMA
                     KRYKGGKLSISDVGGPTANMYGSRCSKGWPVNCRRRSCLFPSICPNLVYDHNPQLEML
                     QDVEKVPGVGHVFIASGVRHDMAISSPAYIKALASKYAGGRLKLAPEHSEPQVLRQMG
                     KPGREVFEAFSKEFYSYCQKAGLKRQIVPYLIVGHPGSDLESAKALRAWLMRHKIKVE
                     QVQEFTPTPMTISTCMYYTGMDFETGEPIHIPKPGEVRKQKELILWHQK"
     gene            complement(32141..32353)
                     /locus_tag="PLO35_00645"
     CDS             complement(32141..32353)
                     /locus_tag="PLO35_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOH78198.1"
                     /translation="MKGRVKWFNKNKGYGFIITEDNKEYFAHWKSIVTNSPMELKVLE
                     QDEMVTFDLMDTDKGIQAINIMRLNA"
     gene            32539..32895
                     /locus_tag="PLO35_00650"
     CDS             32539..32895
                     /locus_tag="PLO35_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH78199.1"
                     /translation="MDANILLVDDDRLLREVIGEYLSSSGYKVDLAEDASIALAKFNP
                     GKYHLALIDMVMPGMNGLDLMKEIMQRDPNIFCLIMTGYPTIDSTYKAMVEGASDYII
                     KPFQLIELLHIIQKYA"
     gene            32916..33818
                     /locus_tag="PLO35_00655"
     CDS             32916..33818
                     /locus_tag="PLO35_00655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH78200.1"
                     /translation="MYDIELEIPDPTRVASPGALWSHPVVMTEAARLQDLEPRLLVAR
                     KGQFRAAELPLYERRRLGSVTLQTASGSYYQGLRCWHQQESPAPRRLLDELQISSAIA
                     VWLKDRYRRLLFNLSPENTDVRGFTWNLLKATPLYTFIQDLSLDIHPLRDEREKLRRA
                     DERGYSFECRLDVDAFLELSDAMFERKGHHAYTRADKMKDYITSLHQAGLLEQYNVCL
                     DKRVVSANILLSGSDDLAYTVLRASHRDEMRNGVSLWHTSQLLQSLKGRYQKLDLCGA
                     NVPEVARFKAAMGLSLVAFYRISS"
     gene            33903..34832
                     /locus_tag="PLO35_00660"
     CDS             33903..34832
                     /locus_tag="PLO35_00660"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOH78201.1"
                     /translation="MKTICVLKGGISSEREVSLVSGTAIANELRTMDYDVFELDPADH
                     PRLDELLTAIKTRHADLVFIGLHGGLGENGKLQAALELAGIPHTGSGFEACCVTMDKY
                     LSKLTASAEGIPTPAYILMREDLVNDYNTAKDLQGFLSGTGMPVVVKPNDGGSSVGIS
                     LVREIGDLKQAVKLAFREAKHVLVEQFIPGRELTVTVLDGQALPVVEIKPLQGWYDYK
                     NKYTKGNTEYIAPAEIEPATAQLVQLYATRLWHAFGLKGYARVDFRYDGSQAWFLEVN
                     TLPGMTSLSLTPMAAKAAGISFSGLLERIINLS"
     gene            34850..35995
                     /locus_tag="PLO35_00665"
     CDS             34850..35995
                     /locus_tag="PLO35_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78202.1"
                     /translation="MKRLFCLLSVLLILMMPLSARSSFSAQFIPSQVFLNELRPAIFN
                     PDQPESQPLLTTLQITNNSPDAVYLAMKLEIMWNDTSIISKEFVSKEPVSSGLWYPLT
                     NADLINSTGSGHFNDISDDEIDFLEVIEGNQVLKDAALSGYFPDGDLKFQISLKDSIL
                     ARNYDDPVTFVARVRNTGVIHLITPGNRIGNIPSQVSMKPVSFVWNAVDTGYNKAWIT
                     IREYPPNIQPNSGSVASTGTVIYETPDATAAADLARSFNFAEFLAFNEDHYYAWQITM
                     DKYDEFNLLNLDPPSRRSKSPTGINTISSVWNVFRYVSDASSQQVSNELQAILNQLNN
                     LGIQNLFNSGYVPTGLINTEGRNYTGTEAVRKVEEIMDKELDIRLED"
     gene            36006..36710
                     /locus_tag="PLO35_00670"
     CDS             36006..36710
                     /locus_tag="PLO35_00670"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOH78203.1"
                     /translation="MKKLIIIAVLSILAISLLNASAVGLITASKGKVSLTRSAKKISF
                     KIGDTINDKDEIRTGKESFASYKYVDGVSSIKVFSNSYVVVTASRSGKSMNKNANVKS
                     GSVYTKVTPGKKGSMKVTTPTTVASVKGTEVFTAVNLAEEATYIVVKGTVTVEVPETG
                     ESKDVGAGETAFVDKDLNLEVKETTQEDLAKLDAAEQQAIAESQPKRIRIQLANEEGQ
                     IKYIEIFYSDPADQSE"
     gene            36715..39162
                     /locus_tag="PLO35_00675"
     CDS             36715..39162
                     /locus_tag="PLO35_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78204.1"
                     /translation="MKKILLGILILSLFSLAMAQTTQRIIAQNQSPLTYIFGQTPLMR
                     VEINQGLEEITEIRLRYRIASETAENRVYLQEAMKPQAPDSPVWEAFLPGDYIADYDI
                     EYYFEFKLADLSVEVMPWEYDINSPFLITPGKMKGRIEEGFVLLSQDDEIDQSDGFVF
                     AVSFFEIADEIEPNSLKVWVGGKDVTRKAVISENTIVYRDKRSRAGEVKAFVTARIEG
                     DDVQSHTWKAKVQGGKFKLPVKFNGSVNFASNVYDYQYTNVDSLSTVFESRNDWTSWG
                     DLTATSGRITAYTNLHLSSDEDRRLQRVNRYTVGLKTPFLELAAGDYTPSLSQFTMNN
                     RNLYGLYGKLFAKYIGLEVAAGEMVRSTYSAAVLEDSTNVMITAPSATFRQEAIGARL
                     RLGLEEGLSIGINGIRNRDIKSSLSTDHYMYTTATGDTVISVTPKDNLVLSIDARLNI
                     PDQNVLLGMEVAGSLLNNNTLDSLITSQDIEDMVPDLPDIDPGELSQLFWLNTNLEPL
                     MPSRNNLAWMAFFRTFFWNNLFHINYSVTGSAFNALGTYYQQNDTSVISITDQFYLGR
                     VLTLSGGYNLTSDNQSGTYAEQNKSTAWFVQSLFRFPNLPYLKASFFTNNTHNINNAD
                     IQPDTLFIPYKRNANNFSVGLGYDFKQIPIVPSQLDITYRASADSKNQGDDEVQIYEN
                     FSNSINVTMSNRMGIIPLRTQVVFSLSNQERKLGLSDPTIDSQFKDDNYTLFAKAEYA
                     LFKNRLIPYVTFRRVHLTGDQEEQSFDYLSLGIDASPIKDMSVSTSISQKYHRFEDLD
                     QQDNDTFTWRFQISQRF"
     gene            39384..41243
                     /locus_tag="PLO35_00680"
     CDS             39384..41243
                     /locus_tag="PLO35_00680"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOH78205.1"
                     /translation="MKYLKLVIPPLGILILIKLLFLVGFFGRLEYMAQDSLFRIRGPQ
                     PISEEVVVVGIDDPSFNALNERWPFPRSHYAKLVENLTRAGVRLIVFDIEFTEPSHHE
                     HDEFLAQTAAAHQNVIFAGKIIDSGGVGIHQQLYSPIAPITDQGLSWGVVNLGVDKDG
                     VMRKYALFHDFDKEPHYTIGVVALANSRVYQEEWSKHIKREDRTLKVAGKAIPLYNYN
                     EALINYFGPARYFPYLSFSSVLDDSTMAMPGQQGVETDDFEEILASGFLKDKIVLVGA
                     DTAELHDYFTTPFSSKDWTPGVEIHANFIEMVLQGKYLAHINMWLYFGIEALLLLLLW
                     FIFRKLKPQWGAILVLVFILAHFAASYLLFLKLGLLIPIVQTALIFLLIYLAGLINHY
                     LASLKEKRFIRNAFQQYLAPELVDKLLRDPKSLKYGGSLQEVTVLFSDIRSFTTYSEN
                     HKPEETVQILKEYLTAMVNTIVDNEGILDKFVGDEIMALFGTPVPLPNPALSACKTAL
                     QMRDRLTELQQKWREEGREDFEIGIGINTGPAVVGNLGSEQIFDYTAIGDTINLGARL
                     EGINKEYKTAKHIIISEFTLEKVQDLVETRYIDEVKVKGKNKAVKIYELIGLK"
BASE COUNT        10081 a        11075 c        10581 g         9406 t
ORIGIN      
        1 cagacaatga gataaacgca tattttatcc aggggctttg aactccaaga ggtgctttcg
       61 cgcaagctca gagcccgtgt tacaatacac atcttctcat tcctcattgt attgaagcct
      121 cggttttctc tgctttctcc gctttctctg acgtttgttg gggttttcac tggatcagga
      181 gatttctgaa gtagcaggaa aaatggaagt ctgcgccctg ggttatacta ctccagccct
      241 caccagatcg tgcatgtgca acatccccaa caccttgtcc tgatcgtcga ccaccggcag
      301 catggtgatg gagtggtctt ccatcaggcg caaagctgaa actgccagat cagttggacg
      361 cagtcttttg gggttggggg tcatgcactc ccttaccggc aggttcaaga ggccatcagg
      421 atggatctgc agttgcctgc gcaggtctcc atcggtcaaa atgccgagca gaatctcttt
      481 ttcatcggta acggccgtgc agccaagctt tccggaagtc atttccagaa tggcgtcttt
      541 catggaagcg gagccctcca ctacaggtag ttccgggccg gaatgcatca aatcgcttac
      601 tttcaggatg agcttcttgc cgatgctgcc gcctggatgg aagcgggcaa aatcattgag
      661 gctaaaattg cggctcttca gaagcgcgat ggcaaacgcg tctccgaggg ccaatgccac
      721 tgtcgtgctg gaggttggca ccattccaag aggctcatac tcctgaggaa cgctgcaatc
      781 cagggccacg tcagccgctt cggccaaagt ggaattcatg ttgccggtga aggctatcag
      841 cggagtcccg atgaacttta aaaaggggat gattgagata agttccgtac cggaaccgct
      901 gttggaaatt gcaattacga catctgagct ttggatcatg cccagatcgc catgaatccc
      961 ttccgcggca tgcaaaaaga tggacgcggt acctgtgctg gccagggtgg cggatatctt
     1021 gcgggctatg atgccggtct tccccactcc ggttagcacc accttgcctt cacagccact
     1081 taacagctca aaggccgtct ccacagcctg ttcatcaagg cgggcggcca tcttgaggat
     1141 ggcgtcggct tcagttttga gttcctgaac gattccggct ttgatgttca cgaaatgtct
     1201 gatcctctta tcccttttcc ctgatgagtt ttacggcttc gtcaaagctc atcactccca
     1261 gatcgccttc ggtatggcgt ctcagagtca gcgctccgct ctcctcttcc ttcttgccga
     1321 tcacgcacat gtaggggatc tttttcatct ccgcttcgcg aatcttgtag ccgatcttct
     1381 cattacggtt gtccacctca cagcggaacc ccaagagagc gaatttctcg ctgagcttat
     1441 gagcgtaatc gaactgagcg tccgtgatag gaatgatctt tatctgtttg ggacacagcc
     1501 acaaaggcag attaccgccg tgatattcca gtagcgtggc aaagaagcgc tccacggaac
     1561 ccagcacggc gcgatggatc atatagggac ggtgggcagt gttgtctgag ccaatatatt
     1621 gcatgtcaaa gcgttggggc agattgaagt caaactggat ggtggtgcac tgccaggcgc
     1681 gaccgatggc gtccttgatc ttgatgtcga tcttggggcc ataaaaagct ccgccacctt
     1741 catcgacgtc atacttgagg tccagcttgg cgaggctgtt cgcgaggctc tgagtggctt
     1801 tgtcccagtc gccctgctct cccacggaag attcaggctt ggtgctgaga tagatgagga
     1861 attcctgaaa gccgaaatga cgcagcatgt ccagagaaaa gatgatcagc ttttccactt
     1921 cttcatccag ttgatcgggc cggcaaatga tgtgagcgtc gtcctgagta aagccgcgca
     1981 ccctcatcag accatgtaag acgcctgatc gctcatagcg atagacggtt ccaagctccg
     2041 ccagacggat aggcagttcc cgatagctat ggtggccggc attatagatg gagatgtgga
     2101 aggggcagtt catcggtttt atatagtatt gctggtcttc cacttccatg gcggaataca
     2161 tgttttcctt ataaaagccg aggtgaccgc tggtctgcca cagattgctg cgtcccatgt
     2221 ggggagtgta gaccaggtca taaccgcggc gcaggtgttc gtctttccaa taggcctcga
     2281 tgaggtgacg gatcatggcg ccgttgggat gccagagcac cagcccggcg cccacttcgt
     2341 cgttgatgga aaagagatcg aggtctttgc ccagcttgcg gtgatcccgc agggcggctt
     2401 gttccaaaaa gtccagatat tcggccagct ctttgttgga ggggaaagag atgccataga
     2461 tgcgtttgag catcttgttc ttttcgtcgc cgcgccaata agcgccggat gtctttaaga
     2521 gtttgacggc tttgatcttg ccggtgctgg agatgtgggg tccgcggcat agatcgatga
     2581 aatccccctg ctggtaggtg ctaatggtat ctgtatcagg gatctctgag aggatttcca
     2641 atttgtagtc ctctcccatt tcagcaaatt tctccatggc ttcggctttg ctgagttccc
     2701 tgcgctgata atgcagatcg gatttactga gctctttcat gcgctcttca atcttgacaa
     2761 gatcttcatc cgtaaagggt tcgtcgcgat caaagtcata ataaaaaccc tgttcgatgg
     2821 cgggaccgat ggtgactttg acgcctggga aaagttgttt gacagcctgg gccatgaggt
     2881 gagcggtgct gtgccagtaa atctctttgc cggcgctggt cttgaaggta tgcagtatca
     2941 gatcggcatc ctgttcgata agatgatcaa ggtcgcggag cttgccatct acttcagcgc
     3001 aaattgccgc gtccgccagg cgaggtgata tgtcattggc cacctgtaaa gccgaaacgg
     3061 gctgctggta ttccttttgg gaaccgtctg gtaaagtaat ctttatattc atgatatctc
     3121 tatatctaac aatagcttca gccgttcgta acactggctt cagccggtcg tagcgctgac
     3181 ttcagtcggc gcggaccatc aagcccgcaa tcttctctga tccagcttct gagacccgaa
     3241 actgaagcat gcttttttgt caagcccggc gtgtttgggc tgcttggttt cccgcagata
     3301 atgcagatta aaatgcagat tgcgcggatt atagcaccca gattgatcgg attacactga
     3361 ttttataaag agggtttaga agctagagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnc cttctcaact tcttttatca agaatctgtt gaatctgtgt gcaatgtcaa
     3541 aatgctatat gcgatatctg cgttcaaatc tgcgatatct gcgggaaaat aacgtcatct
     3601 gcgggaaata ttaataccat gccgggtggc attcagcacg gccagcaggg tcacaccgac
     3661 atccgcgata atggcttccc agagtccgct gatgccactg actcctaaga ccatcaccag
     3721 agtcttgatc cccagggcca ggataatgtt ttggctcacg gtacggtggg tttcgtgggc
     3781 ctgtttgaaa gcttccacaa gctgagcggg acggtcttga agcaaaacca catcggcggt
     3841 ttcgatcgaa gcgggattgc caatctgtcc catggcgata cccaggtccg ctcttgccag
     3901 ggcgggagcg tcattcatgc cctcacccac gtaggccgtc aggcctgtcg agcctgccat
     3961 ggtcgcttcc aattgctcaa tcttctgatg gggtagcagt tccgccgccc aggcatcaag
     4021 tccgagttct tcactgagag cttgggcttt tgcttcccgg tctccggaaa gcatgatcat
     4081 tttgtttaca ccccgaccgc gcaaatcact cagagcagcc ttcatgccag ggcgcaactc
     4141 atccgtgaag ctcatgcatc ccagatagat atcgttcttg actgcatgga cggcgctcat
     4201 gccatcgctg tcaatcagat cgatgaatcc ctgctctttc ataaacaccg gagatccggc
     4261 tatcagacgc tcctctccat agagcagcaa tactcctttg ccgggatatt cggaataggc
     4321 gtttaccatt gcgctgtcgt agtcggctgt gtaagcctgg cgaaccgctt tggcgaaggg
     4381 atgattggag gtgtattcac agcggtagat agtgtcgatc aggagctctg ggcttccatc
     4441 ttccggagta acgagcttgt cgatcttcag ctctccggtt gtgagggtac cggtcttgtc
     4501 aaagaccatc gttttcacct tcctcagcac atcaagatag actccgccct tgaagacaat
     4561 ccctttgcgg gcagccttac cgattcccac gtaataagat agcggcactg agatcaccag
     4621 cgcgcaggga caactgacga tcagaaacac cagagcgcgc ttcagccaaa ctgaagccgg
     4681 ccagcccagc agagtaggaa ccagaaacac cagcagcgcc gctgccacca cggcaggagt
     4741 gtagtatctg gcaaactggg tgataaacct ttcgctggga gatttgcgcg tgctggcctc
     4801 ctcgatgagc ttgagaactc tgcttacggt gctttcggca tccagcgagc tcacttccat
     4861 ctcgatcaaa ccgtccagat ttagggtccc agccaggacg cgatcgcccg ctcccacagc
     4921 caggggagct gcttctcctg tcaaagtgga tgtatccaga gcggaattgc ctttgcggac
     4981 ctcaccgtcc agaggaaccc tctcgcccgg ataaattagc aggacgtctg cgatttcaat
     5041 tttatccagg ctacagtcca cagttccttc ctcagtaagc ttgtgggcct tgtcaggttt
     5101 gatggccaac atgcctttga tggcggaacg ggatttggcc acggccagcg actccagcca
     5161 ctgaccaatt tcatatagca ccatgactgc cgcggcctcg gtgtattcag ccagaaacat
     5221 ggctcccgcc gtggcgacac tcatcagaaa ctgctcggaa aagacctgcc ctttgccgat
     5281 tgatctgacc gctgagatta acactctatg ccccaccagg agccatgaga tcaggcccaa
     5341 agccacactc atacttcctg ataggcctgc gccgtaagag attatcagca gcatcactcc
     5401 cagcaggagc ggcagccaaa acctgaggtc tctgctcttt tcttcaacgg cgtctccttt
     5461 tactgtgatc gacacgcccg gctctatgga gtttgccagg tagttcagct tctcgatgac
     5521 gttttcaatg tcttcattgt agcttacgat cagacgtttg ttcacaaaat ccaactgagc
     5581 gtttgtgacg cctggaagtt cgtttacgac tgattcaatc ttattgccgc agtgagcgca
     5641 atcgagattg tggatatcat actcccgcgt cctcatcttg agctaccttc gtggacatgc
     5701 tccagcgcga cttcgaaaat ggaacgcacg tgttcgtcat ccagagagta ataataggtg
     5761 gttccgtctt tgcgaacctt gaccaaacga ttcattctga gcactttaag ctgatgggat
     5821 acagcggatt ggcccattcc aaccagagtg gccagatctg ccacgcagag ctcgtgccgg
     5881 gtgagaaaat acaagatctt caagcgggtc ccgtccgcga atacttgata aaagtcccgc
     5941 agccgttcta tggttcctgc atctggaagt tcacgtctgg cttgttctat ctctttgtca
     6001 tctatcattt tgcataaaca gggcgtaatc atgtcttgct ccttatatga atatgtgttc
     6061 atgtatcgtg aaagccactt ttatggcaag tctttttttg aggatttcca cgtttcgctt
     6121 gacaagctcc cgcgtccaaa aagacaagtc cgatattgaa tatattatcc aacaatgagg
     6181 tacataatgc gttacgatgt catcatcatc ggtgccggtc cagccggatt gagtgccgct
     6241 atctattccg cccgcggtgg actcaagacc gctgtctttg aaagagcttt tatcggtgga
     6301 caaatcactg tgactgatga agtggaaaac tatcccggct ttgaagatgc gctatcagga
     6361 ttcgatctga cagaaagaat gcgggcccag gcagccagat ttggcgcgga gtttcacgac
     6421 gaagagatta ccgcgatggg catggagggc ttatgcaaga tcatcgagac cggcaaggac
     6481 aaataccgcg ccaaagcagt gataatctgc acgggagctc atccccgccg cctcaacgtg
     6541 ccaggcgaag aacgctttac gggaagggga gtatcctact gcgccacctg tgacggcgct
     6601 ttatacagag acaaagtcgt cgcggtggtt ggtggtggag acagcgctat tgaagaaggg
     6661 atctttctca cccgttttgc caaaaaggta gtagtaatcc accgtcgcga tcagctcaga
     6721 gctcaacaga tcatccagca gcgcgctttt aaaaatccca aaatggagtt tatctgggac
     6781 agcgtggtgc aagagattca gggagaggct atggtcgaaa agctggagat ttacaaccgc
     6841 aagaccaatg agatcagcta catccctgcc gatggcgtgt ttatctacgt gggcattctg
     6901 cccaacaacg aactgctgga atcacgcata gagcttgatt cagcgggttt tgtgctcacc
     6961 gacgaattca tgcataccaa cgttcccggc atttatgccg cgggagatat tcgtcacacg
     7021 gtgctgcgtc aggttgtaac tgctacttcg gatggtgcca tagccgcatg gagcgctgag
     7081 aaatggatca tagagaacta cgacgagatc gaaggcatgg agagcaacca cagtgccgag
     7141 tagcagagat atccgccagg cttttataga ctttttccgg gagcgtggac accagtttgt
     7201 gccctcctca ccagtcgtcc cacaggacga tcccaccttg cttttcgcca atgccgggat
     7261 gaaccagttt aaagatattt ttcttggact cagagacgct gagcatcccc gagcggcaaa
     7321 cagccagaaa tgtatccgcg ccggcggcaa acacaatgac ctcgaggaag ttggcacgga
     7381 cggctaccat cacaccttct ttgagatgct gggcaactgg tcgtttggag attattataa
     7441 acaggaagct atctcctggg cctgggaact gctgacagga gtgtggggct tgcccaaaga
     7501 ccggctctac gccacagtcc atcacacaga ccaggaagct tacgagctgt ggaaaacgca
     7561 aaccgacatc gaacccagcc acatcagcta ccatggcgac aaagacaact tttgggaaat
     7621 gggtgaaacc ggtccctgcg gaccctgcag cgaaatccat atcgatcgcg gagaaggcca
     7681 ttgcgataaa agccacatac ctggtcacgt ttgtgacgta aacgccggct gcgcaagata
     7741 catcgagctc tggaacctcg tcttcatcca gtataaaaga gaggcggacc gcagtctctc
     7801 tcctctgaaa aaccgctttg tggataccgg cgcgggcttg gagcgcatca ctcaagtgct
     7861 tcaggataaa cgcagtaact acgagaccga cctctttatg ccaatcatcg agcgagtcgc
     7921 ggaactatcc ggccagacct acgatcccca aaagggagtg tctcaccgag tggtggcaga
     7981 tcatatccgc tgcctctgtt tcgctttagc agacggtggt ttcccttcca atgagggcag
     8041 gggatatgtg ttgcgccgca tcctcagaag agcggcccgg catggcagac tgctgggctt
     8101 cgcggaaccc tttctgcatc agttggtgga cagcgtcata gcagtgatgg ggcatcatta
     8161 caccgagttg aacggcaaac aggaatacat caagaccatc atcaaagcag aggaagaacg
     8221 ttttaatcac actctggata caggtctgga acgcttcgcg gagatcaaat cacgcctcga
     8281 gggcgatacg atcgacggca aagacgcttt catgctctat gatacctacg gtttccctct
     8341 ggacctcaca gtcatcctgg ctaccgaaca gggccttaaa gttgacttgg agggcttcga
     8401 agccgaaatg acacatcagc gtcaaagagc gcgttctgcc tccagattca gccttcagac
     8461 cgaggacatc gaatggatcg ttttgaatga gctaagcccc actcagtttg tgggttacga
     8521 acagcatcag tgtgactgcc acatccagcg ttaccatatc tcggaagacg gcaaaatcca
     8581 tctgcaactg gaccggacac ccttttacgc ggaatccggt ggccagatag cggatacggg
     8641 tgagctgcta agcgagcagt ttcaggtccg cattcaagac gtccgcaagc agggcgatga
     8701 gtttatccac agcggcagga tcatttcagg cgcacctcac ccgcaaatca tgcatgccag
     8761 gatagatgaa gcccgccggc aggaaattgc ccgcaaccac actgccaccc atctcatcca
     8821 tcgcgccctc agagatatcc tgggcgaaca cgtgcagcaa aaaggttctc tggttgctcc
     8881 ggaatatgcc cgcttcgatt ttacccatct gaaagccctc tcgagggacg aaatagacaa
     8941 gctggagcag caggtcaacg atgtcattct gcaaaaccgc caggtccgca cggatctgct
     9001 tcccatcgaa gcagccaggc aacagggcgc gatcgctctc ttcggagaaa agtatggcga
     9061 cgaagtaaga gtggtgagcg ttcaggactt ttccaaagaa ctctgcggtg gtacccatgt
     9121 gagcgccaca ggtgaaatcg gcctcttcaa ggtgctgtct gaaagctctt ctgcggctgg
     9181 aatccgaagg ctggaaatcg tcaccggagc gcacgcgctc cactatgtaa actcatggca
     9241 ggaaaggctg cacagcatgg ccggcaagct taagaccagc gacaaactgc tggaagacaa
     9301 actggaagcc ctgatcgcaa agaaccgcga gctcgaagaa gaactcaaga aacagagcgc
     9361 caaacaaggc ggcgaacagg tgaacgaact gctgacccag gcggaaaacc tgaacgggtt
     9421 ccgttttctg gcggtggaaa tacaggctga aaccgctgag cagcttcgcg gcctgggaga
     9481 tgcgttgcgt gataaggccc ccgatctgct ggccttcctc tttgcaccgc agtctgacaa
     9541 ggtttccatt ctctgcgtgg caggccccaa acttggcaac cgctttcacg ccggcaaact
     9601 ggtgggctcc gtggcggctt ttctggatgg caaaggtggc ggcagaccag attcnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcagat atgacaagca acgcagtctc
     9781 cgtcaggcta tcgtggcgca tcgatctgat caatccatca attccaccgt ccagctctac
     9841 tattccgctc tggacagatt gaccggaata gcacccgatt ccttcaaacc ccctcagctc
     9901 cggttaccgg atatctcatt gaccccggag cggatgccta aggggatacc cggcaagcgc
     9961 ttgatcggca tttttccggg agcggcccac cttaccaaaa tgtatccgga gcagcagtgg
    10021 agggagttct ttcaaatcgc ggatgcggac tggagttttg tgttgctggg gagcctcaaa
    10081 gaatccagga tagcggatag gctgatgacg gctttgcctg atcgaatcga gaaccgttgc
    10141 ggaagctata gctttgagga acttgcgctt gtgatgagcc aatgcgatct gatcatcagt
    10201 gcggacagcg gacctatgca tctggccgct gctctaaact tgccacagat cgccattttt
    10261 ggcagcactc atccccgcct cggtttcgcg cctcaaaatc ccaaagcgat cgtcctatgc
    10321 gcggacctgc cctgtcagcc ttgcacttta cacggccagg attactgccc gttgggccac
    10381 tttgaatgca tgaaaaagat cagcccaggg atgctgaagg ataaggttgt gaaggtttta
    10441 ggggtataga gctttcgggt ttagaccacc tgccatctca accggataat aatctgcgca
    10501 atccgcgtaa agatctgcgt aatctgcggg aaatgcatag tccgtgggaa acttacacat
    10561 acgctaaatc cccgtttcca tctcggctat tctgtctctc agttcggcgg ccctttcaaa
    10621 gtccagtttg gccgccgcgc gctgcatctc ttttttgagc agggcgatca gcttatcgcg
    10681 gttgtcgatc tccaggtact cgctgaagtc agccttggag ggtttggtct catcatcctt
    10741 gtcggcatcg atggaagcgt atccttccgc gattgaggtg gactgcataa tctgttcgat
    10801 gctcttttgg atggtctggg gtgtgatgcc gtgttccgcg ttgtatgcca attgtttaac
    10861 ccgtcggcga ttggtctctt ccagggtttg ggcgatagcg tcggtaatct catcggcata
    10921 gaggaccacc ttgccttcca cgtttctggc ggcccggccg gaaatctgga tcaatgacct
    10981 cgcggagcgc agaaaacctg tcttgtcggc atccaggatt gccaccagtg acacttccgg
    11041 aagatcgaga ccttctctga gcaggttcac cccgacaatc acgtcaaact cacccaagcg
    11101 caacccccgg atgattttgg agcgttctat ggagtcgatt tcactgtgca gccatttgct
    11161 tctgatgccg gcattgttca aataggtgct caggtcttcg ctcatacgtt tggtgagcgt
    11221 catcaccaga gccttgtgtc cctttccgac acgctctttc acctgggcga tgagatcgtc
    11281 cacctgatgt ttgatgggtt ttatctccac ttcggggtcc accagtccgg tggggcggat
    11341 cacctgttcc accacgaccc ccttggtgag ttccagttca tagtttgccg gtgtagccga
    11401 aacgaagatc acatgccgca ggtaatgctc aaactcctca aagcgcaagg gacggttatc
    11461 gaaagcggag ggcagccgga acccgtattc caccaggttt ttcttgcggg tgaaatctcc
    11521 gccatacatg ccatgcacct gggggatagt gacatgcgat tcatcgatga taaagatgaa
    11581 atcctccgga aaataatcca gcagacagga tggaggagag ccagccggag tgccggtcag
    11641 gtgtctggaa tagttttcga tcccggagca gtaacccaat tctctgagca tttcaaggtc
    11701 aaacatggtg cgttgcttca gccggtctgc ctccacatag cgttgattgt ccagataata
    11761 cttcacccgc tcttccattt cgcgttcgat ggattgcagg gccccgcgca tcttgtcctc
    11821 attcatgata aagtggatag cgggatacac gggatagctc tccacgctct caagcacttg
    11881 ataggaaatg ggatgcagct tctggagagc tactatttca tcgccataaa actccactct
    11941 caagcagtgt tccaggtagg cgggatagat gtccacgatg tcgcccctca ccctgaaagc
    12001 cccacgttca aaagcgatgt cgttgcggct gtagtgagcc agcaccagtt tctggatcag
    12061 ctcgtcgcgc tccattctgg cccccacatc cagtcggatc aaagcctccc gatattcctc
    12121 cggaaccccc aatccataga tacaggatac cgaggccacg atgatcacgt cccgccgctc
    12181 catcaggctc atcgttgccc tcaggcgcag cttttcgatc tgctcgttga tatcgctgtc
    12241 cttttctata taaatatctt tgccggggat gtaggcttcc ggctgatagt aatcgtaata
    12301 ggagataaaa tactccacgg cgttttcggg aaagagctgt ttaaactcgc catagagctg
    12361 agcggcaagg gtcttattgt gggagagcac caagaccggt cgatcgaaac tggcgatgac
    12421 gttcgcgatt gtataggtct ttccgctacc agttacaccc agcaaaacct gatgcggcct
    12481 tgcttccctg atcccggata ccagttcctg aatggcctgg ggctgatctc ccgagggctg
    12541 atactctgat gccagcttga agattgacat ccttggtcct ctctttcttt ctttataatt
    12601 gccattgatt taaccgcttt cggaaagcgg ggctggaaaa gtcttaatat tctgcttgac
    12661 gtatagcttt gaattcaaaa gctgggatac acaaatcgcg tatggcggtt tttgtccata
    12721 aaataactga catggagtca tagatggtaa acctcaacga tctgcagatc aatttcgaca
    12781 acctgctcaa gctcggatac gcagtgctcg atatccgttt caaagaatat gacctttgcc
    12841 ctgactctca caaactggtg atcgccaggg tcgattccga ccgcgatgaa ttctatcagg
    12901 acatgctgaa gcagtacact gcccaggagt tcaaagccaa tgaagtgagc gaaatctgga
    12961 ctgacatcct caagcacaag gtcaaaatga gctccgtgct caatcgcgac atcgccatca
    13021 aagtagccgc tctggattac atcgagaccg tctataacgc ctaaatgacc gatcgctccc
    13081 tgctcctcat caagcccaac gcggtgcagc atcatcatgt gggacacatc atctccatcg
    13141 ttgaggacca tggcttcaag ctgagggata tcaagatctt tcagttcact tccgccagcg
    13201 cggaagaatt ctacgaagct caccgcggca aggaattctt tgagcggctg gttcgcttca
    13261 tgtgttccgg tgagactatc gccctcctat tggaacgtaa tgacgcggtg gaactgctgc
    13321 gggagctggt gggagacgcc gatcccgcca aacgagctcc tcacacgatc agagcgtcct
    13381 atgccgaggg agtaaccgag aacgccgtcc acgcttcgga cagcatcgct gccgccaagc
    13441 gtgagatcaa gctgatattc ggagctaact actagactga caaatatatc caggttaaga
    13501 cgcgtggcta acaccacgcg tttgtttttc tctggatgct tcgcccttgc ccgtgcatct
    13561 tatgcccagg cataccaagc ccagaccatc cccaatctca ttggggatgc cttgggggag
    13621 ataagagtgg ccacaagcgc aaacgcaaag agaggggagc cgaagctccc ctcaaatgat
    13681 gccaacgtgc ttcacttcaa gcgtttggag aggtggtccc tgagcagttt ttcaatccgg
    13741 cgctgcctgg tttcaggcct tttggcttca ttgatccaga gcatctgttc ctttttatgc
    13801 gtgtaggatt gggtgataaa cgcttcccat ttcccctctg cttcgatggc ggccttaacg
    13861 tcttccggta catccacttg ccgatcctgt acatcgcgtt cgaggatcat gtggacccgg
    13921 tcgccggcct tgaatcctgt ggcgtcccgc atctcacggt tgaagaccat catgtggctg
    13981 ccactcatgg gggccagact actgcgaaac tgatgctccc ccaccttgac gattactggg
    14041 acgcgggctc tagtgccaaa cacagcgtaa acgtcgaaat cgcaaacaac aaaggcggag
    14101 ctgggatact catccgtaga ttggagcagg atgtccagtt ccacccggtt gggcgcttca
    14161 atatcaggtc ttgctgggct caaggagtct tttccccttt cagcacctga ctggaatcac
    14221 tcacgcagcc cgagctcaga gcagggcaac cactgcaggg gctgttctga gaaatgccca
    14281 cgatctcgtt gcgcatcgct ttgtcaggct ttactttgga caggggagga cggattgtct
    14341 ccacctgtag ggaatagcta actatcaagc cagcaaacac gatcgagacc atgctcatca
    14401 gcttgatcag aatattgatg caggggccgg ctgtatcctt gaaggggtcg cccaccgtgt
    14461 cacctacgat ggtggctttg tgagcgccgg aattcttgcc gccgtgcttg ccggtttcca
    14521 catacttttt ggcgttgtcc caagccccgc cggcattgtt catcatgatc gcaagcacaa
    14581 agcccgcggc caaagcgcct accagaacgc ccaaaactcc cgctacaccc aaaagcatgc
    14641 caaccaccac aggggttccg atccccacca tcgctggcag caccatctca tgttgagcgg
    14701 ctacggtgga gatttttacg caggacttgt agtccggttt gctggtgcct tccagaatac
    14761 ctggaatggt ggtaaactga cgtctgacct catccaccat tttgcctgcc gctctgccca
    14821 ccgctttgat ggtgagagcg gaaaacaggt aggtcaccat cgcgcctata aagatgccca
    14881 tcaggaactt gggattgagc gcgctgatct gatagtagtt gataaagtcc tgtatggaag
    14941 cgtgttccac actcacgaca tcggaagaca ggcggctgta gtggatattc ataaaaaggg
    15001 tcttgcccac cttgtctccc agaagcagga ggccggctct cacttcttcc agatacgcgg
    15061 ccaaaagggc catggctgtg agtgccgcgc tgccgatggc aaaacctttt ccggtggcgg
    15121 cagtggtgtt gcccacgctg tcgaggttgt cggtaagctc acggacgtta tcgtgcatgt
    15181 gagacatctg agcgttaccg cccgcgttgt ctgctatggg gccgaaggcg tccatggcaa
    15241 gcgtcactcc aagcgtggcc agcattccca ccgctccaaa accgattcca tagagtccca
    15301 tctcgatgga ctggaatcca tggctgcaga tgaaggccag catgatgccg accgcgaccg
    15361 taatcacagg tgccgcggtg gacctcatcc ccacggatat gccttccaga atcacggtag
    15421 cgggaccgaa tttgccctgc tcggcaatgc tctgagtggg cctgtagctg tgagacgtgt
    15481 ctctttcggt aaaccaaccg atcaggattc cgatcgccag accgatgacg ctggagagaa
    15541 acacgccaaa gtaataatcg ggaggcagga gatacctggt cacaaagaac gaagcgacgg
    15601 cgatgagagc ggagctggaa tagacgctct tgttgagagc gaacatcaga tcgtgggtag
    15661 tggccccctc cttggtcctc accataaaga tgcctacgat ggacaggatc gctccaattc
    15721 cagccaggat catgggcgcg atcacaaagt tgagctgcca gaccttaaaa ctctctccca
    15781 gcatagccac agcccccata cccagagcgg cagtagccag gatggaacca gcgtagcttt
    15841 catagaggtc ggctcccatg ccggccacgt cacccacgtt gtctcccacg ttgtcagcta
    15901 tggtagcagg gttgcgggca tcatcttcag gaattcccgc ttccactttg cccaccaggt
    15961 cggcccccac gtccgcggcc tttgtaaaga ttcctcctcc cagacgggca aatagcgctt
    16021 gggtggaagc gcccatccca aaggaaagca tgatgacggt gatcttttgg agcgggacct
    16081 ggaagtgatt gagaataaaa aaccaggcgg agatgtcgat caaagccaaa cccaccaccg
    16141 tgagccccat cacgctgcca gcccggaagg ctaccttgag tccctgattg aggctttcag
    16201 agcaggcttg agcggtgcgg ttggaggcca gagtggccat gttcatgcca ataaaccctg
    16261 ccaggccgct gaaaaatccc cccgtcagaa atgcccaggg caccaaccaa tgcagcacgc
    16321 caaggcccag ggccataacc tgaaacacga taaacgcgcc aatgaagaaa ataataacac
    16381 ccttgtattg ctgctttagg taggcaaacg ccccctctct cacgtagcca gcaatctcta
    16441 tcatcctcgg agtaccggga ttctgagctt ttacctgata aaaaaagacg agcgcgaagg
    16501 ccagggccag cgtggctccc aggggggcga gataccacag gatggggatc aattccatat
    16561 tcaaaacctc gataagatcg tttccaattt gtagttacag gcgttacctg aagaaaccgc
    16621 tctctgttaa caaagcaagc ctatttgtca atgtttttaa atggctggaa gcaccaactg
    16681 aagaaaatac ttgccaaaac cctgtctgcc aatttacctg tccggcaata tcattgcgtg
    16741 aggatagtat gtcatacaga cgccttatca tattgctttg tttagtatta gcctgcagcg
    16801 gccagctcgc ggcactgcgt tttgcccgct ggaatacctc aatggggtcg ttcacggctg
    16861 agctctatga ccacctggta cccataactg ccaacaattt tatctccctg gccaacagtg
    16921 gtttttataa tgagctgata tttcaccggg tcgtgcatgg ctttgtgatt caggatggct
    16981 gtccctatgg cactggttac ggaggtcctg gatacactat tccggatgaa tttcatccag
    17041 acctcaatca caacgaagct ggaatcctgg ccatggcgcg caccagcgct cccaattccg
    17101 ccggcagcca gtattatttc accctcgcgc ccgctcctca tttgaatgga agttatgccg
    17161 tgtttggtaa atgcatccag ggtttggacg ttattatggc aatagggaac gtgccagtgg
    17221 atgccaacaa ccgacccctc acacccgtca cgatagacac gctgcggatt ctggatatgg
    17281 caatcacaaa cctgactcct gccaacgacg agaccatcga gtgtagcgcc aatgaggagc
    17341 agttcttcat cgtggaagcc agcagccaga ttcttccgat cacctacaga tggtttgtgg
    17401 acggggcgga acagcctggc ttcagcgatt tcatctttgg ttacagcttc ggaattggaa
    17461 gccacactgt gagttgcggg atgacaaacg cggactgggc ccacaccgtg acctgggatt
    17521 taaatgtcca ggcgacatat aacagcgatc cggaagttcc atcgcccact tatctgacac
    17581 tcagccccaa tccctttaaa gagggcaccc gcgtagactg gaaaagcccg tcaaatgatg
    17641 atctgagcat ctacgacctg aagggacgcc tgatcagaaa gatctctgga gcttctggca
    17701 gctttgtctg ggatggcagt gacagccgag gaaaccgctg tcctgctggg atttatctct
    17761 ttaaggcagg ggagcgaacc gagaaaggac tcttgctgaa ataggggctc agggcttgac
    17821 gcccagttcc tgaaagagct gttccagttc agccttgagc cgctgagcgg aatagcggtc
    17881 gctgatatcc tgccaggcct gagctgtgat ccggtcgcag agctctggat cttccaaaac
    17941 cttcatcagg cagctggcga agccgtcttt gtcctgatga gcaaagagcc agccgctttg
    18001 gccgtctttg atcagattgg gaacccctcc cacttttgag gccagcaccg gtacgcgatt
    18061 ggccattgct tcgatgatgg cgaccggggt accttcgttc agggagcaga gcagcagggc
    18121 atccatgtca gcatacagtg gtctgaggtc tttgatgaag cctgtaaaga tgatcttcgc
    18181 ctccaaaccc agtagtcgga cctcagcttt gagggattcg ctgagctctc catctcccac
    18241 aaaggcaaat atcacgtcat cgcgttcttt cagaacccgg cgggcgatat cgatgaaact
    18301 aaacgggttt ttgatcgcgg tcagccgccc cacaaagccc accaacttcg tctccacggg
    18361 aattccaagc gcggctttga acattccctg gtgagagtca tcatggatga ttttaccaaa
    18421 ttcaaagccc agatggatga ctcttagctt ttctgcctgg cagatattga agctgagcag
    18481 ctcgtccctt tgttgatcgc tgatggcgat aatgcgagtg ctgattctgg ctagagcgcg
    18541 ttccagattg atggaaacga ggctcatgag cttgccaaaa tatccccgga agttgttgcc
    18601 atggaaggta tgcaggatca caggcacacc cgctaaacga gcggccactc ttcccaccaa
    18661 tcctgctttg gcggtgtgag tatgcacgat gtcagggctc agtctgcgga tgattttata
    18721 cactttgggg atcagcagca ggtcctgcaa gaggttgatg ctgcgcccca tggtgggcag
    18781 gttgatcatc ggaaagtcat attccttggc cagatagagc atgtcgctct cattggattc
    18841 cacatcgccg tagaggaccg tggagctgta aactccaccc tgaaacatct gagaaatctc
    18901 caccacatgg atagaggcgc ctccaatgtt cagacgggtg atgatattga ggatcttgat
    18961 catgagatgc ctgtcatctg ttttatagcc tgaagcgttt cttcgacggt gatggcgttt
    19021 atgcagcggt agccataatc gcatgtcctc tggttgcagc cgctgcaggg aaactcgtga
    19081 aagatccaac ggctctgcgt gcccagcgga ccactgatgt gggggaaatt gggacccagg
    19141 aggccaagcg tgggcactcc agccagagcg gaaatgtgca tggggcctga atcgttgcca
    19201 agaaagaacc tggcggctga gatcagctca aagctctcca cgaggctcat accacccgcc
    19261 ttatcgataa tggccttgct tttagagaag gccctaatcc tcagattgat agccttatcg
    19321 gcttcatcgc caatcagata caccgtgtcc acataagcgc tttgcaacag ccattctgcc
    19381 actttgcaga agttatcctc tttccagcga cgtcctggaa accgcgctcc ggcatggaaa
    19441 acagcgtagt tccgcgcttc tttgagcctg ggaatactca gagacgtcac ttccagatgc
    19501 ggaatcccca aagccgcgga aatcgctgcc ggcctttggg caaggtgaca aagccgcata
    19561 tcctggcgga agcggttgac cctgattcca gcggcttcta gtgtgaagct gttcaaatag
    19621 cggtttgtga aactcagatc attcacgtaa ccgataatgg cgcgggcgtt tgacaagatc
    19681 gccaggatgg aattgcgggt cagccatccg gggatgatga tcaggtcgca gttcagcttc
    19741 cagagctctt ttaacgtagt ccagtagtcc tgatgggcaa tatacttatc aaattggaag
    19801 tagttacgct gcagatcaca gaagggagcc ggaaagcagg ctgtgaggac agcctgagga
    19861 taacgagccc gcagctcgcg aaagaagaag ctgtcgcaga ggatgtctcc catcccgctc
    19921 agatagagga cgcagatgct cttgggctcc agattggaga agcgggtgaa agcctgacgg
    19981 attttccaca gcagccacac tagcggcatc accatgattt ttaggtagtt gcgcgcttca
    20041 ctcatacaag gcagccagtc gttccgtgag ataagagcgt cgaaagtttt gcaggatatc
    20101 aggccggcag cgataacttt ccgggtgctc cagaagtctt ttcagagcag ggatgatcgc
    20161 ttctgaatcc tgcacccaga catacactcc ggcaccgctg gtttcgatca agtccttcac
    20221 caggccctgt ttaggtccca gggccagaat gggagggcca agtctaagca gttcaaaaac
    20281 cttgccggta tagactcctt ctgagacctt gtcatcattg agcagcagca ggttaaccgc
    20341 agcacccgct gcgatcgaca gagcttcccg atgggttttg tatcctttaa aattatagaa
    20401 gtcatagcca ttacgttcaa tctgagctcg ggcgtccaga gccagggacc ctatatggat
    20461 gaggggagta ctcagatcag agcccttgag agcatccagc agaggtaaga gattgggcct
    20521 gttaagattg tatatctttc ccatatagac cagcgtcggc gtctcaaagc tctgcctgac
    20581 aaggtcggta aaatcatcct cgtcatagcc gttggtgatc agttctgccc ttttactcat
    20641 ccagggatat gccttaatat attggcagcg aatagattcg gtcacaaata cggctttgtc
    20701 gcagtgtctg aggacttgct catccatcca gcggataaaa cgcatcctcc tcttcggcaa
    20761 cactgattca atcagaggtt caaactgcca gccatcccgg taatccgcca cccagaaaag
    20821 cttgtctccc aggcacttct tcaagaggat acccaccagc atcgcggaaa agggaaaagc
    20881 ggtgatgtag aggtttctga gcttgtaacg cttgataaaa aatagcgcat agataaaggc
    20941 aaaggggacc cagccaatct gtttgtcaat tgggaagagc atgtgattca gcctcatcag
    21001 cagtcttgaa ctaagtccct taatgctatg ggatacagac ttttgcctga tcgatgccac
    21061 cagatcgaca ctttttcctc ttaagcccca actgggagtg cggatgattc tggcgctttg
    21121 gggtatttcc cggcataatg atttgtccag cacagtttta gtaggttggg aaggagtgac
    21181 caccaagcag tcccagcctg atctggccag gtatttgaga aacttgaggg gtctttgcac
    21241 tccgctttcg cctaccggag gaaactcatt gatcagcatc aggatccgct tatccttcat
    21301 acacttctct cagtttggcg atcaggctat ccatcataaa actaccgcgc accaattcct
    21361 gtcccttttc ggccatagct ttggcctctt cctgatgctc catcatatat ctgatcgcgt
    21421 cactcaaaga tgcctgattt ctgggttcgc agagcagtcc atgctccccc gatgcgatca
    21481 cgccgtcgat gccctgtccc ttcacgccga tgattggaat gccggcatcg agggcttcca
    21541 gatagacgat gccaaaggtc tcgctatagc tgggtaaagc aaagaggtcg aagtacttat
    21601 atgtctggcg gataacttga ttggccttgg cggggtagat ggtgacctga cgttccactc
    21661 ccaggcgcaa agccaggtcc agcaagcgtt ctcgctcttc cccatcgccg atgatcttca
    21721 gggaaacccg cttgccctga tatctgattt cagccaggga gcggatcagg atatcgagac
    21781 ctttttcccg tttgagattg gctactgcca ggatgttgaa gtcctcctta tcgaggctgg
    21841 ccgccaactc cttgatgaaa agatcggtac atggctgagc gggaatgatc gcattaccga
    21901 taaacatgct cttctccagc gggacgatct ccttcatcct ttggcgcaag ccctcattga
    21961 cgaaaaagac tttgtcccaa tggtcataag cttgggccag ggtcttgcga taaatgtatc
    22021 tcagataggc atattccgcc ctttcaggga gggtcttgat attgtggacg ctgaggtatt
    22081 tggctgcgct acagcttttt aacacatctg caagacaggt aagctcggga acgtgacggc
    22141 aatcatggac gtgaataata tccggccccc agcttgaaat caaagcttta agccgtggaa
    22201 tcacccactt gcgatactgc aacatagtga gcggaaacag cagccggctt tgaggatagc
    22261 ggatataaac aagctcaaat tccggagtct gggtgattct gagctccggc tgcgagggaa
    22321 accaggtggc cactactttc acctgataat gacgcgccag ggcctcagct tgatggcgca
    22381 caaaaatccc cgccacggga ctttgctcgt ggggaaacat gtcggtgatc agaagcagct
    22441 ttttgggttt cataaacatc ttagttcctt ttctgggaga ggcctatttt gtcaatcgcg
    22501 gagttgagat gcctgtctct tgatcgcgaa gccaaaaaaa ctcctttaca aaaaggcttg
    22561 atcggcaaaa gtgtctctgt tgtcagttgc agagaggctc tactctcatt gccggcaagc
    22621 aaatttgatt atgtaactat tcgcaaatcc atgcgtttgc gatcacatca ggagattaaa
    22681 tggataagat cagacttgga ttgatcggct gcggccgaat ttccaagaat cattttgacg
    22741 ccgtttccca gatcccggag gccgattttg tagcagtagc ggacgtgatt gaagaaaaag
    22801 ccaagaccat ggccgcaagc tatggcatca cctccgttta taaagactat cgcgagatgc
    22861 tggaaaaaga acagctggac gcggtgagca tttgcacccc gagcggtatg catcctcaga
    22921 tcgggatcga cgtggccaat tcaggacgcg ccgttatcac agaaaaaccc atgggaatca
    22981 gcatcgagca ggccgacaaa ctcatccagg cctgcgatgc taacaacgtc cacctctttg
    23041 tggtcaagca aaaccgtctc aattccactc tccagctctt aaagcgcgcg gtggaaaagg
    23101 aacgtttcgg acgcatctac atcgcgcagt caaatgtttt ctggcaacgc ccccaagcct
    23161 attacgacgc agaaaagtgg cgcggcacct gggaatttga cggcggggcc tttatgaacc
    23221 aggccagcca ctatgtggat gccatctact ggctgctggg caatgtggat agcgtgatgg
    23281 cttacactgc cacgatggcg cggcgcattg aagctgaaga caccggctgc gccattctcc
    23341 aattccgcag cggcatcatt gccaccctca acgttactat gctcacctat cccaagaatt
    23401 ttgaaggttc catcaccatc atcggtgaaa agggcaccgt aaaagttggc ggtatggccg
    23461 tgaacaagat cgagaaatgg gaatttgaag actatgacga cgacgaccgc atcgctcagg
    23521 acgccaacta ccagcctcca aacgtctatg gttttggcca caacccctat taccgcaacg
    23581 tgatagacgt cctgctgggc cgcgatactc cctctaccga tggtcgcgac ggacgcaaat
    23641 ccgtggagat cattcaagcc atataccgct ccgccaagac gggaaaaagg gtgtccttac
    23701 ccctctaagc tccctttagc ctcctttact ccacctatgc cccaccttta catctccttt
    23761 gcccaggcac aggagatcca caggtggggc gcatgatatg gctttgtggg tttcagctct
    23821 ccactcgcgg ctggagacgg atggcctgat aaaaagatat tgactgcaat cccgcttcca
    23881 gatcagatgg tctgtagctc tgaattcgat agaggagata cacatgcgca ggtttatcat
    23941 catcctgatc gccctggccg tcctgtttgg cggctgtggt aaagcgatcc agtccaacaa
    24001 ggtaaaagtc acgttctggc acgggctgag cggtccgctg ggagacactc tggctgaaat
    24061 gatcagagaa tttaaccgca cccacaaaga tattgaggtg ctggccaatc ccatcagctc
    24121 ctatgccgct ctttcccaaa agcttatggc ctcgatccag gcaaaaaagc aacccgatat
    24181 cgctcaggtg tttgagtcct ggacagccaa atacgtggcc gccgacgtgc tggtcaatat
    24241 ctccgatctg atcaaagagg accctgggtt tacggaagcc gattttgaag acatgtatcc
    24301 cgtctttcgc aacgccgtca cctaccaggg caatatctgg agctttccct tcaataaaag
    24361 cgtgagggcc tatttctata ataaggacgc tttctaccgc gcgggactgg atcccaacca
    24421 ctttccccag acctggcagg aattcagaga ctactgcaaa accttcacca aagacgccaa
    24481 accccgcaag atctacggga caaactacgg ccccaacgag tggcagttca taaatttgct
    24541 gcatcaggcc ggtggcagga ttatcgacga caacggccaa ccagtcctca acagccctgc
    24601 tggagtggaa gctctcacct atgtgacaga cctgatcaat aaagacaaga ccgtctatac
    24661 cgtgaaggaa tatgatgggc aaaacgactt cctggccggc atcgtggcca tgtatgaagg
    24721 ctccagcgtc tccatcaccc atatgcgtca acaacccatc aatttcaata tgggttacgc
    24781 gcctttgcct acatatcgca ccgctcagag tgccatcagc ggtgccaaca tcgtgatctt
    24841 caaaagtgga gactccaaac gcgaaaaagc cgcctgggag tttatcaagt ggtttacgga
    24901 tacggctcag accgccaagt ggagcgtcaa gacctgctac atgcccgtcc gcaggagcgc
    24961 gatggagact aaggtcgtta aggactttct ggctgagact cctcagttcc aagggatatt
    25021 tgaccagctt gaacaagccg tttacgagcc tcaggtaccc gcctggttca acgcccgcac
    25081 agagctaaaa ggctttttgg agaaggcctt caaacaggtc ttgactccca aagaggccct
    25141 cgaccaggca gctgccaagt tcactgagct gatcgccgcc gaagaggtcc agaaatagcc
    25201 acaacagatc taatggaccc aaacagccag atgaggatta tgagagatgg gcctttttga
    25261 tcgcgctctg agcagtctca ggttgcgtct caatgccgcc atcattggca aggccctgat
    25321 cgccacgctg ctgatatttc tactgggcct gcatctgtat ttcatagcct ggattgggtt
    25381 tagtcctcaa tctccgctct tgccatacat caatatcaca ctgcggatcg gcatggcttt
    25441 gatcgcgctc tttatcctga tcacagctta caataggctt cttagtcatt atcaggtagc
    25501 cagatggatg gaccttagcg ctgaccatgg agacgatctc tatcaaaacc tgttgcagtt
    25561 acgccaacag ggtgaaagcc aggaagtgct ggaagtgctg gccctgggag ctcaaagcag
    25621 actgggagag cataagtatc cccctccacc ctggtacaaa cccacgcatt ttttcctgat
    25681 tctcttcttt ctggtgggca tgatcagcgt gtggggactc tcctatgaca gtttccgcct
    25741 ggctttgaaa cagttttact ccaataaagc tgaaagtata gactacaaac ggacggtgga
    25801 agtcagcccc ggcaatctga cgatcggcaa gggacaggag ttgatcatca gtgtgctgaa
    25861 tcccgatccc agactggagc accggctttt tttcagatat gacaaggctt ggcgtcaact
    25921 ggccatgacc gacttccgct atcgctttga agctctggaa tatgacatcg aatactatgt
    25981 ggccaatgag gttgcggctt ccgacacttt caggatcaaa tgtctggatg agccttttgt
    26041 gaagcgctgg gaactgcgtt atcagtttcc tcctcacacc agtctggctg ccaggaccga
    26101 tactctttct ctgggcaaca tcgaagcata cagacacacg ctggtgagct tgaatctggt
    26161 taccaatatc cccgtgaaga ctgccgtgat gagctttgcc gacggcaaca agatagatct
    26221 gcagggcgct ggcaacagct tcagcactca gatcagaatc aatcaacccg gcacctggta
    26281 tctggagctg gtcgatgagc tgggccgtcg caaccgtcct gaggaaaaga cgatcagcat
    26341 catcccagac gcccctccgg aagtcaggat cgtcttcccc ggggaggata ctctgctgga
    26401 ccagcggatg aacctgcctc tgatcatatc agccaacgat gattttggcc tgcgtgacct
    26461 cagtctgcgc ttcaacatca acgacacccc cacgcgggag ctcatgatca ggtccgtgat
    26521 gggcggtaag atccataatc aggactatct ctttgacctc aagccatttg agctatttcc
    26581 cggggaccgg gttacctatt gggtgcaggt gtgggacaac tccccagacc gccagaaagc
    26641 tgagagtgcg cgctacgtgg cccgctttcc ttccatagcg gaaatctatc aggagatcga
    26701 acgccaggaa agcctcaaga cctcagaact ggaacgcacg ctggaagata cgcgcgatct
    26761 gcaaaaggag tttgagcaga aacggcgtga actgctcaaa gagccagacc cctcctggga
    26821 agacaaaaaa caactggagg atatccttca gaagcaggaa cagcttgccg accaggtaga
    26881 taacgtggcc gaggattttc aggaactgat cgaccagatg cgcaacaatc aggccctttc
    26941 tcccgaaacc ctgcagaaga tgcagaaaat ccaggaattg atggaagaga tcagcaacga
    27001 cgagcttcgc gccgccatgg agaagttcaa cgacgctctc ggaaaaatgg accaggaagc
    27061 actgcgtaag gttatggagg acttcaagtt ttccatggaa gacttcgccc agcgcatcga
    27121 tcagaccctc cagcttttgg agagcataaa gaaagagcag gcggtggaaa aggccctgca
    27181 gatgtctcag gagatcgaaa aatcccaggc cgctctgaag gagcgcagcg gcgataccaa
    27241 acagaacccc gaaagcctgg cccgtgatca ggaaaacatc gcctcccgct acgaagagct
    27301 caaaaaagaa ctggaaaagc tttcagacat gatggacccg gcccgcgatc aagaagtcac
    27361 gcaacagctt gaacagctta aaaaggacat gcaacaaagc aagccggaac aggacatgaa
    27421 gtccagcgct cagcagatgc gccagaatca aagacaagat gcccaacaga atcaaagcag
    27481 cgctcttgaa aagatgcggc gcttcactat gaagctggct gaaatgaaaa agtccatggg
    27541 cggcggctct caaaaagaag tgatggaagc catgcagagg gcgatcagag agctcctgat
    27601 cttctccaag aatcatgaag ctctcaaagg acgtctggga acagacccct accccatcat
    27661 gcaggagctg atcgctcaat acgatggcct ccagatcctc ctgaacaagt tcttttccac
    27721 tccccaggtc acgatgttta tccctcccaa gttttatctg gacctctcag acaccaaccg
    27781 ggcctacagg gaagtgtttg taaacgtgag cgatatgcag tattatcgtc tgcctgaact
    27841 gatgagtggc atccagaagg gcctcaacct gatggtctat gatctcatgc aggccctcaa
    27901 caatccatcc cagggtggag gaggcggcgg aatgcaatct ctgatgcaga tgctggaaca
    27961 gatgggacag gaacagatgg cgatgatgat gctcacggag cagatgatga tgcagctgca
    28021 acaacaaggc ggcagaatgg acgcggccat gcagcagcaa atgcagaaat tggcctcgga
    28081 ccaacagcgg ctggcagaca acctcaaacg cgccctgcaa aacgatccgg aagctcagaa
    28141 acagggcaat gccatcaaac agatcattga agaggccgaa gctgtggccc ggcaactccg
    28201 ttccaatcag ctttctaatg acttgatgcg gcggcaggaa aacatcattt cccggctctt
    28261 ggacgcccaa cgctccatca ataagcgcga caccacccaa aaacgcaaag gcgagacctc
    28321 cactcagcag tttcctcaaa aagccggtga gatcgatctg aaagaactcc gccgtgccgc
    28381 catgctggac gatagctaca agagcctgcc ggcagcctat caacaggtga tcatcaaata
    28441 cctcaagtat ctgaacgaca aagctcaatg acacgctaca tgagaatctg cctgatcaac
    28501 tttatactaa ttatcattgt ctcagggctg tggggccagt atgacgagaa gcagatcctc
    28561 acccagcagg cccagaattt ctacaactcc aggcagtacg ctcaggccga gactatttac
    28621 aaacaagtac tggagcgctg gccgggagac caactggcca tcctgcagct gctcaacatc
    28681 gcctatcaaa ccggcaacat cgacaaagcg gaagctatcc tcaacgaaca cggcaggtat
    28741 ctgcaacccg ccaccgttct ggaccatgag ctgcaaatcc tggtgcagag aggatcggtg
    28801 gacgccgcct gggaaaaagc gctacgcagt ctggagctta atcccaaaga ccagaaccgc
    28861 tatcggatca tcgcgtccta ttttgagcgg cgcggcttct atgatcaggt cctcagactc
    28921 tacgagcttg gcaggcatca gtttaacaac ccggagctct tcagtctcga gtatgccaat
    28981 tccgctctca actatcagcg ttatgagctg gccctccggg aatatttacg ctggctggac
    29041 aggaatcccg gcaatctcta ctttatcaac aaccagtgca aattggtgct ggaagcagac
    29101 agcagcctgg tctatgtgat cgaggaagcg gcagggacgg gtagtaatcc tgtgatgaaa
    29161 gagctgctgg ccggcactct ggtgagcaga aagcgttaca acgacgcgct gaacgtctac
    29221 aaagaaatgg gacaggagaa gctgctgctc tttgccactg aacagtttaa cgccatgaat
    29281 gatgaagtgg cccagcctgc ctttgcatgg ttggccggcc gggaagcaga catttggcgt
    29341 aaatcccagt acctgtacaa tctggctgtg attggcaccc gcaatcaacg ctacacggat
    29401 gccgattcat tggtcacagc ggtgatcaac gattcgctcc tcagtctgcc ccaaaaccgg
    29461 tggcgcaacc cggtcagcta tcaggcccgc aagctcaagg ccgagttgag tctgatcctg
    29521 agccgcgatc ctcaggaggc cttgaactgg attgctcagg ccaaaagttt tgccagaaac
    29581 gcccctgaga ttcaggaatg cgagctggaa gcagcccggc tattgatttt ggaccaggat
    29641 tatgaggctg ccagccagaa actggcactg gtcacagagg ccagattgat cgaaaaacgt
    29701 gaccatctga gctttctgag agccctgctg agcggtgatg tggccctggc ggacagcctg
    29761 atgaacgaat acgtgatcac gtggccggag gggcaattca ccaacgacgc tatctatctg
    29821 atgatgcacg cgctggccct gtcggatgag gccagagcac attttatggc ggcttatcgc
    29881 gccaatcagc tccgcgatcc cttcgccccc accatgctga tcactctctt cgcgggcact
    29941 caggatgagg agcttctgat cctggcagtg gaatgggccc ttgccatggg agataaagcc
    30001 caggcactca gcattttgtc tcatgattgg caggatgagg tgtccaaaga ctacgcggcc
    30061 cttttaagct tgttcttgca ggaaagcggt gaagaacagc aacgccaggc cagggagttt
    30121 ctgagacaga atcccaacag cgtgttctct cccagcttta gaacgcgact gatcagggaa
    30181 tcgcggggca aaccggaata gaggaaccgc agacgtcctc gttcacgtgg cataactcga
    30241 tttaatagca gcagttacag atcctctcac caaacctgct gagccaccct taccagttgc
    30301 tctcatgccc agaaggagca tcccctaccc atcccgaatg tagttgggga tgagtaaggt
    30361 gagatctgga gaggcaaaag atggatgagt aagggcgaag gcaggggact atttctggtg
    30421 ccagaggatg agctcttttt gtttgcgaac ttctccgggc ttggggatgt ggatgggctc
    30481 tcccgtctcg aaatccatcc cggtgtaata catacaggtg ctgattgtca tcggggtggg
    30541 ggtgaattcc tgcacttgtt ccaccttgat cttgtgacgc atcagccagg cccgcaaggc
    30601 tttggcggat tccagatcac tgccgggatg acccacgatc agatatggca cgatctgccg
    30661 cttgaggccc gccttctggc aatagctgta aaactctttt gaaaacgctt caaagacttc
    30721 cctgcctggt ttgcccatct gcctcagtac ctgcggttca gaatgctcag gggccagttt
    30781 caatctgcca ccggcatatt tggaggccag ggctttgatg taggcagggc tggaaatcgc
    30841 catatcatgt ctcactccgg aagcgataaa gacgtgtccc actcctggca ccttttcaac
    30901 gtcttgcagc atttcaagct gaggattgtg atcataaacc agattggggc agatggatgg
    30961 aaaaaggcag ctgcgacgtc tgcagttcac gggccagcct ttggaacagc ggctgccata
    31021 catgttggcg gtggggcctc ccacatcgga aatgctgagc tttccaccct tgtaacgctt
    31081 tgccataata acagcttcgg ctctgatgga ggctttggaa cgggaactca cctctctgcc
    31141 ctgatgagcg gcgatggcac aaaaattgca gccgccataa caaccgcggt gagaagtgat
    31201 ggaggttttg atctgttccc aggctgggat gagcttgttt tgatagatgg gatgcggagc
    31261 tcgctcaaag ggcatagcgt agatcctgtc cagttctgcg gaattgagag cggtggcggg
    31321 agggttgtgg cggaccagtc ttccagcatt gacctgatag agcgtctggc ggtcatgctc
    31381 gctccagaac agcctggaag ccttccagaa cgtacgtttg tccacacatt cacgatcgga
    31441 aggcagcagc gtgcccggct ctggaatctc tgtttttgat gcaaagacaa gcgtcccggg
    31501 aatatctttg agagagtcaa cggagcttcc ctgccccaga gctgaagcga tctccagaat
    31561 agccctctcc cccattccat acaccagtat atcggcctta ctgtccgcca ggatggatga
    31621 tctcactttg tcctgccagt agtcgtaatg cgccagacgg cgcatcgaag cttccaggcc
    31681 gcccagaacg actgggacgt ttttgaatag gcgtttgagc tggttggtgt aaacaatgct
    31741 ggcccggtcg ggacgttttt ccggaagacc ggaggggctg aaggcatcgt cgttgcgtcg
    31801 ctttctctgg gcggtgtaat gattgatgag ggaatccatg ttgcccgcgc tgacaccaaa
    31861 aaagagcctg ggagcaccga gcctgagcaa atcggcatcg ctgtcaagat caggctgagc
    31921 gatgattccc actctgtaac cggccgcttc caacactctg ccaatcagcg ggatgccaaa
    31981 ggaagggtgg tccacatagg cgtctccgct caccaatatg atatccaggc agccccagtg
    32041 gcgggaagcc atatcttgtc tgtttatcgg taaaaaggcc atgtaaaaca aagggcccag
    32101 ctcttaaaag ccggaccctg taaccataat ctaaaatgta tcaggcgttc aggcgcatta
    32161 tgttgatagc ctgaatgccc ttgtcagtat ccataagatc aaaggtgacc atttcatctt
    32221 gttccagcac tttgagctcc atcggggaat tggtgacgat ggacttccag tgcgcgaagt
    32281 actctttgtt gtcttcggta ataatgaatc cgtatccctt gttcttgtta aaccacttaa
    32341 ctcttccttt catgcgtaac ctcattcata ttcttggttg ccactgatat tatagttagg
    32401 tttacgtcaa tacaaaattc ttttttctac actcccggaa aaatttctaa agaaaccgca
    32461 tcatttgggt tgacagttta accctatgga atagattagt acgcacataa agaatccttg
    32521 aggagcgaga ttgtaagtat ggacgcgaat attcttttag ttgatgacga ccgtctgctc
    32581 agagaagtga ttggtgaata cctttcctcc agcggttaca aggtcgatct tgctgaagac
    32641 gcgtctatcg ctttggccaa attcaatccc ggaaaatacc acttggcgtt gatagatatg
    32701 gtgatgccgg gcatgaacgg tctggacctc atgaaagaga tcatgcagcg cgacccgaat
    32761 atcttctgcc tgatcatgac aggttatccc accatcgatt ccacctacaa agccatggtg
    32821 gaaggagcct cagattacat tatcaaaccc tttcagctca tcgagctgct ccacatcatc
    32881 caaaaatacg cctgaactcc gctggtgagc ataatatgta tgacatagag ctggaaatcc
    32941 ctgaccccac tcgagtagcg tcccccggag cgctttggtc ccatccggta gtcatgacag
    33001 aagcagccag gctgcaagac ctcgaacccc gattgctggt ggcgcgcaaa ggtcagttcc
    33061 gggcagctga attgcctctt tatgagcggc gacgcctggg aagcgtgact ctgcagacgg
    33121 cctccggttc atattatcag gggctgagat gctggcacca gcaagagagt cccgcgccca
    33181 ggaggcttct ggacgaactg cagatcagtt cggcgatcgc cgtatggttg aaagaccgct
    33241 atcgcaggtt gctgttcaac ctcagtccgg agaatacgga tgtgcgtggt tttacctgga
    33301 acctactgaa ggccacacct ttatacacat ttatccagga cctttcgcta gacattcacc
    33361 cgctccggga cgagcgtgaa aagctgcgcc gcgcggatga aagaggctac agctttgagt
    33421 gccgcctgga tgtggacgcg tttctggagc taagcgacgc catgtttgaa cgcaagggcc
    33481 accatgccta cacgcgggcc gacaaaatga aagattacat cacgagcctc caccaggccg
    33541 gccttctgga gcagtataac gtctgcctgg acaaaagagt agtgagcgcc aatatcttgc
    33601 tgtccggatc agatgatctg gcctacaccg tcctgagggc cagccataga gacgaaatgc
    33661 gcaacggagt gagcctctgg cataccagcc agctgttgca aagcctgaag gggcggtatc
    33721 aaaagctgga cctttgcgga gcgaacgtcc ctgaagtggc acgcttcaag gctgctatgg
    33781 gcttgagttt agtggcgttc taccgcatca gttcatagta ccttgggtct ccaatcgctt
    33841 ttttttgttg acggaatgtg acaacccatt tatagagtct aaaaagctta ttaagaggta
    33901 agatgaaaac gatctgcgtg ttgaaaggcg gaatatcatc cgagcgggaa gtatctctgg
    33961 tgagtggaac ggcgatcgcc aacgagctcc gcaccatgga ttatgacgtg tttgagctgg
    34021 accccgccga tcatccacgt ctggatgaat tgctaaccgc gatcaaaaca cggcacgccg
    34081 atctggtctt cataggcttg cacggaggcc tgggtgaaaa cggcaaattg caggccgctt
    34141 tggaactggc aggcatcccc cataccggca gcgggtttga agcctgctgc gtgaccatgg
    34201 ataaatatct ttccaagctc accgccagcg cggaagggat tcccactcca gcttatatat
    34261 tgatgcgtga agatctggtc aacgattaca atacagccaa agatctgcag ggctttttga
    34321 gcgggaccgg catgccggtg gtggtgaaac ccaacgacgg tggttcttcg gtgggaatca
    34381 gcctcgtgcg ggagatcgga gacctcaagc aggctgtgaa gctggctttc agggaagcca
    34441 aacatgtgct cgtggaacag tttataccgg gacgcgagct taccgtcact gtactggacg
    34501 gccaggccct gcccgtagtg gagatcaaac ccctccaggg ctggtacgat tacaagaaca
    34561 aatacaccaa aggcaacact gaatacattg ccccggctga aatagaaccc gccacggctc
    34621 aattggtcca gctttacgcc actcgtcttt ggcacgcttt tggcctcaag ggctatgcca
    34681 gggtcgattt tcgttatgat ggttcccaag cctggttttt ggaagtaaac acactgcctg
    34741 gaatgacatc gctgagcctt acacccatgg ccgccaaagc cgctgggatc agcttttccg
    34801 ggttgttgga gagaataata aacttaagtt gataatcaag ggaggtccca tgaaacgcct
    34861 tttttgcctg ttgagcgtat tgctcattct gatgatgccg ctgagtgcca ggagcagttt
    34921 tagcgctcaa ttcattccat cgcaagtatt tctaaacgag ctacggcccg cgatcttcaa
    34981 tcccgatcag ccggaatccc aaccgctgct gacgaccctg caaatcacca acaacagccc
    35041 tgacgcggtc tatctggcca tgaagctgga gattatgtgg aacgacacgt ccatcatctc
    35101 caaagagttc gtttccaagg aaccagtgtc ttccggtctc tggtatccac tcaccaacgc
    35161 tgacctgata aactccaccg gatccggtca cttcaacgat atcagtgacg atgaaattga
    35221 ttttctggaa gtcattgaag gcaatcaggt gttgaaggat gcggctctgt ctggctactt
    35281 tccggatggc gatctcaagt ttcagatcag cctcaaggat tccattttag ccagaaacta
    35341 tgatgatccg gtcacctttg tcgccagggt ccgcaacacc ggagtgatcc atctgatcac
    35401 ccctggcaac agaattggca atatcccttc ccaggtgagc atgaagcccg tcagcttcgt
    35461 ctggaacgct gtagacacag gatataacaa ggcctggatc actattcgtg aatatcctcc
    35521 caacatacag cccaacagcg gttcggtggc cagcacggga acagtcatct acgagacccc
    35581 tgatgccaca gccgcggcgg acctggcccg cagctttaac ttcgcggagt ttctggcttt
    35641 caacgaagac cactattacg cttggcagat cacgatggac aagtatgacg agtttaatct
    35701 gctcaacctc gatcctccca gcagacggtc caagagtccc acggggatca acactatctc
    35761 cagcgtctgg aacgtgttca ggtatgtgtc agacgcttca tcccagcagg tgtccaacga
    35821 attgcaggca atcctgaacc agctcaacaa cctcggcatc caaaatctct tcaactctgg
    35881 ctatgtgccc accggcctga tcaataccga aggacggaat tacaccggta ccgaggcggt
    35941 cagaaaggtg gaagagatca tggacaaaga actcgatatc cggttggaag actgagggag
    36001 gaaccatgaa aaagttaatc atcatcgctg ttctcagcat cctggccatc agcctcctga
    36061 atgccagcgc cgtaggtctg atcaccgctt caaaaggaaa ggtcagcctc acccgctctg
    36121 ccaaaaagat atccttcaag atcggtgaca ccatcaacga caaagatgag atccgcactg
    36181 gcaaagagtc ttttgcctcc tacaaatacg tcgacggcgt ttcttccatc aaggtattct
    36241 ccaattccta cgtggtggta accgcttccc gcagcggcaa aagcatgaac aagaacgcca
    36301 acgtcaaatc cggcagcgtc tataccaaag tgactcccgg caagaaaggc agcatgaaag
    36361 tcactactcc caccactgtg gcgtccgtga aaggaacgga agtgtttaca gccgtcaacc
    36421 ttgcggaaga ggccacctac attgtggtga aagggaccgt gaccgtcgaa gtgccggaaa
    36481 ccggtgaaag caaagacgtt ggggcaggtg agaccgcgtt cgtggacaag gacctcaatc
    36541 tggaagtgaa ggaaactact caggaggacc tggcaaagct tgatgccgct gaacagcaag
    36601 cgatcgcgga aagccagccc aaacggatcc gtattcagtt ggcaaacgag gaaggacaga
    36661 tcaagtatat cgaaatcttc tattccgatc cagctgatca aagcgagtaa tgccatgaaa
    36721 aagatattgt taggaatcct tatactcagt cttttcagcc tcgctatggc ccagactacc
    36781 caacgcatca tcgcgcagaa ccagtccccc ctcacctaca tatttggtca gacgcccctg
    36841 atgcgggtgg agatcaatca gggtctggaa gagatcactg aaatacgtct cagataccgc
    36901 atagccagcg agactgctga aaaccgggtt tacctgcaag aagccatgaa acctcaggcc
    36961 ccggattcac ctgtctggga agcatttctg cccggtgact acatcgcgga ctacgatatt
    37021 gaatactatt ttgaattcaa gcttgccgat ctgagcgtgg aagttatgcc atgggaatat
    37081 gacattaaca gcccttttct gatcactccc ggaaagatga aaggccgcat cgaggaaggc
    37141 tttgtcctcc tcagccagga cgatgaaatt gatcagagcg acgggtttgt cttcgcggtt
    37201 tcctttttcg agatagccga cgagatagag cccaacagcc tcaaagtctg ggtgggtggc
    37261 aaagacgtta cccggaaggc cgttatctca gagaacacca ttgtttaccg tgataagaga
    37321 tcccgggcgg gtgaagtgaa ggcctttgta actgccagaa ttgagggcga cgatgtgcaa
    37381 tcccacacct ggaaggccaa ggtccagggc gggaaattta agctgccggt caagttcaac
    37441 ggcagcgtca acttcgcttc caacgtctat gactatcaat acaccaacgt cgacagcctg
    37501 tccaccgtct ttgaatccag aaacgactgg accagctggg gcgacctgac ggccaccagc
    37561 ggtcggatta ccgcttatac caaccttcac ctcagttccg atgaggatcg ccggctgcag
    37621 cgcgtaaatc gttacacggt aggcctcaag actccttttc tggagctggc cgcgggtgat
    37681 tacactccct ctctcagcca gttcacaatg aacaaccgca acctctatgg cctctatggc
    37741 aagctgtttg ctaaatacat cgggctggaa gttgcagccg gagaaatggt gagaagcacc
    37801 tactccgccg ccgttcttga agacagcacg aatgttatga tcaccgcccc ctcagccacg
    37861 ttcagacagg aagcgatcgg tgccagactg cgtctcggac tggaagaagg cctttctatc
    37921 gggatcaatg gtatcagaaa ccgcgatatc aaatcctcgc taagcactga ccattatatg
    37981 tacaccactg ctaccggcga tactgtgata agtgtgaccc cgaaagacaa tctggtgctc
    38041 agcatcgacg cccggctcaa cattccagac cagaacgtgc tcctgggcat ggaagtggca
    38101 ggcagcctgc tgaacaacaa caccctggat tcactcatca ccagccagga tattgaggac
    38161 atggtacctg atctgccaga tatcgatccc ggtgagttat cacagctgtt ttggctcaac
    38221 accaacctgg aacctctgat gcccagccgc aacaatctgg cgtggatggc ttttttccgt
    38281 accttcttct ggaacaatct cttccacatc aattacagcg tcactggctc cgcgttcaac
    38341 gccctcggga cctattatca gcaaaatgac accagtgtga tctcgatcac ggatcaattc
    38401 tatctgggac gggtcttgac cctctcaggc ggatataacc tgacatcaga caatcaatct
    38461 ggcacctatg ccgagcagaa caagagcaca gcctggtttg tccagtcgct cttccgcttc
    38521 cccaatctgc cctacctcaa ggcctccttc tttaccaaca acacccacaa catcaacaac
    38581 gccgacatcc aacccgacac gctgttcatc ccttacaagc gtaatgccaa caacttctct
    38641 gtggggttgg gttatgattt caagcagatc cccattgtgc cttcccaact ggatatcacc
    38701 tacagggcca gcgcggatag caagaatcag ggtgacgatg aagtccagat ctatgagaac
    38761 ttcagcaaca gcatcaatgt caccatgagc aaccgcatgg gtatcatacc cttaagaact
    38821 caggtagtct tttcgctgtc caatcaggaa cgtaagctgg gactgtcaga ccccaccatc
    38881 gattcccagt tcaaagatga caactacacc ctgtttgcca aggcggagta tgctctcttc
    38941 aagaaccgtc tgatcccata tgtcaccttc cgcagagttc atctcaccgg tgatcaggaa
    39001 gagcaatcct ttgattatct tagccttggc atcgatgctt cgcccatcaa agatatgtcg
    39061 gtgagcacca gcatcagtca gaagtatcat cgctttgaag atctcgacca gcaagacaac
    39121 gacaccttca cgtggagatt ccagatctcg caaagattct aaaccattga gtttcccgcg
    39181 gatttcgcag attgaggcac agatttcgca gattattagt atgtttggaa ggtttcgagg
    39241 atttagccct ccagccttct caattgaatc aaaatcagcg agatctgcct aaagatttgc
    39301 gtaatctgcg gataacacac caaatataat acattgcaga gggacgtcag cgtcggcgtc
    39361 ccttggtttt tttaggagcg ttgatgaagt acctcaagct tgttatcccc cctctgggaa
    39421 tcctcatctt gatcaaactg ctattcctgg tcggattttt tggcagattg gaatatatgg
    39481 cccaggatag ccttttcagg atccgggggc ctcaaccgat atctgaagaa gtggtggtgg
    39541 tgggcattga cgatcccagt ttcaacgccc tgaacgagcg ctggccattt ccccgcagcc
    39601 actatgccaa gctggtggaa aatctcaccc gggctggcgt gcgattgatc gtcttcgata
    39661 tcgaattcac ggaaccctcc catcatgagc atgatgaatt tctggctcag actgccgcgg
    39721 cccatcaaaa cgtgatcttc gccggaaaga tcatcgacag cggcggagtc gggatccacc
    39781 agcagcttta ctctcccata gctcctatca cggatcaggg cctcagttgg ggagtggtca
    39841 atctgggggt ggataaagac ggcgtaatgc gcaaatacgc cctcttccac gattttgaca
    39901 aagagcccca ttacacgatc ggtgtggtgg ccctggccaa ttccagagtc tatcaggaag
    39961 aatggagcaa gcatatcaag cgagaggacc gcaccctgaa ggtggcgggc aaagctatcc
    40021 ccctctacaa ctacaacgaa gctctgatca actactttgg cccggcccga tatttcccat
    40081 atctatcgtt ctccagcgtg ctggacgata gcacaatggc catgcctggc cagcagggcg
    40141 tggaaacaga cgactttgaa gagatcctcg cgtccggttt tctgaaagac aagatcgtac
    40201 tggtgggagc cgacacagcc gaactgcatg actattttac cactcctttt tcatccaagg
    40261 actggactcc aggagtggaa atccacgcca acttcatcga gatggtgctg cagggcaagt
    40321 atctggccca catcaatatg tggctctact tcggcatcga ggcactgctc ctgttgctgc
    40381 tatggttcat cttccgtaag ctcaaacccc agtggggcgc tatcctggtc ctggtattca
    40441 ttctcgccca tttcgccgcg tcctatctgc tattcctgaa gctggggctg ctgatcccca
    40501 tcgtccagac ggccctgatc ttcctgctca tctatctggc gggactgatc aaccactatc
    40561 tggcctcgct gaaagagaaa cgctttatcc gcaacgcttt ccagcaatat ctggcccccg
    40621 aactggtgga caaactgctc agagacccca agagcctcaa gtatggcggc tctctgcagg
    40681 aagtcacggt gctgttttca gatatccgtt ccttcaccac ctactccgaa aaccacaagc
    40741 cggaggaaac cgtccagatc ctcaaagaat acctcaccgc catggtcaac accattgtgg
    40801 acaacgaagg gatcctggac aaatttgtgg gtgatgagat catggcgctg tttggaactc
    40861 cggtaccctt gccaaatcca gctttgtcag cctgcaaaac cgctctgcaa atgcgcgaca
    40921 ggctcaccga gctgcagcaa aaatggcgtg aagagggcag agaagacttc gagatcggga
    40981 taggtatcaa caccggtccc gccgtggtgg gaaacctcgg cagcgagcag atcttcgact
    41041 acaccgctat tggcgacacg atcaatctgg gtgcccggct ggaaggcatc aacaaagaat
    41101 acaagaccgc caagcatatc atcatcagcg agttcactct cgaaaaagtg caggacctgg
    41161 tggaaacccg ctacattgat gaagtgaagg tgaagggcaa aaacaaggcc gtgaagatat
    41221 atgagctgat cgggctcaaa tagcctgcgg ggtcttagag ttttagaggg ttgagaaggt
    41281 tgagaaggtt gagaccttct gccttctcaa ccacatatac aataatctgt tagatctgtc
    41341 aaa
//
LOCUS       DAOSFN010000004        37787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_858_length_37787_cov_8.992367, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000004 DAOSFN010000000
VERSION     DAOSFN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 37787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37787
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_37787_cov_8.992367"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1195
                     /locus_tag="PLO35_00685"
     CDS             170..1195
                     /locus_tag="PLO35_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOH78206.1"
                     /translation="MKRAVLICVLITISICLWAGAKKPRASKRPAWVDDPSQLYQPEK
                     YLSTVGIGTSRSDAEENASANLAAILLTEVTASGEEVDRYLQITSGGQTQTSSVSQVS
                     TSIRAQVDQALVKLKFGKSWKDEQARHYTVAYLERTETADIYLGMLADNNARVMSYLR
                     AVLDVTDPWKYHAWLNAAWTIDQHSAALLSQLAVIAPKERSAYQAPYDSEVLKFQATD
                     AARKLQFRFELKTPEAEELRPTLETLLISMGFVIDPYAANLLSCELKLEPVDQLSEQK
                     FFRYLLSLAIIDKDGTQIIGYSDSGREGHLIESQARAGAVSTAAAKLETEFRKTLLDY
                     IDTLARR"
     gene            1376..2116
                     /locus_tag="PLO35_00690"
     CDS             1376..2116
                     /locus_tag="PLO35_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78207.1"
                     /translation="MLRHISLIIAVLALASGTLNAQFSAYVELSGAITGYNDAQSPNN
                     ATTDRFSFIDDLESPAIVPSGRLHIRWNVAPKHQVGLLMTPLTVNADGQFDRSITYEG
                     ETFLPGEDIEAVYTFNSYRLQYRYMFPKLKVLRSLGLSIKVRDAVISLKSDTKYAEKT
                     DLGVVPLISFDLGHRFNDKWEINFEGEALASKFGRAEDVQLSVDYRVNESNEIRLGYR
                     LLEGGSDIDEVYTFALFHYPTIGWSMEF"
     gene            2227..>2744
                     /locus_tag="PLO35_00695"
     CDS             2227..>2744
                     /locus_tag="PLO35_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOH78208.1"
                     /translation="MTHFLKVLIVDAATGFYRIQRYPLGAFFGPVDLGLHLAGKYNSL
                     CIGAGIFAGSIFPGSNRLVFTGFSPCWGGFYISSMGGAGLVWDDLGINLLAIIGKAPQ
                     PKILVLNRDHGEEIDLSLETIDPEAIWNSGDRGVYAMLHHSFDGFKDRYENPPRVLAV
                     GPAAKYTDMGGIV"
     assembly_gap    2745..2844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2845..3928
                     /locus_tag="PLO35_00700"
     CDS             <2845..3928
                     /locus_tag="PLO35_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOH78209.1"
                     /translation="ATNADSLLAFNYQSIHWTDEERKAFHAKHIRNHYLKQFNEETIV
                     PRQFSTCGEPCPAVCKKNHGIYKKDYEPYQTMGPLCGIFDQRAAELLNREADTLGFDA
                     INAGGMLAWMMECLDAGLLKPEELGVDVKPRWNLTDFDTVTDSLHNAQLGVRLLNACV
                     GPDRSVNISEGVRKYARRLARVKGQAIMDRLVINSFARKGWMVPNQYWTPGALAPMPA
                     MGRYYMYYGPQYFEPRQLGKICADRMKTELMLDNLGFCRFHRAWAEEMLPEIVEALYG
                     LGAKLELSSHITARRINCRNASVFWESSRNIDFTRSFLLRHKRQGSSDPSLETWLARF
                     EANPHEAALDWWYEIRKGIDESLREN"
     gene            complement(4144..4821)
                     /locus_tag="PLO35_00705"
     CDS             complement(4144..4821)
                     /locus_tag="PLO35_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78210.1"
                     /translation="MAENTENPTIETPDVLDKPLPEEPLQDAASGTIETPTITKPEAP
                     EAPKPAAPAPAPVAPAPVAPAPKPAAPKPPIHKTSSEIESNEMTRKVSLIELIMILML
                     VGLVLVFIFGMQQMKIDKEKETIAQQKFESTIPVFDRIIKAMEDYRKQDEFGDYPITL
                     DEIGSFPNPDFKFDYSYDDKIITAVSTKEFGKEGVEVKYSLNNSVYEVVDPSPKEKPT
                     VKDDWLP"
     gene            complement(4889..8980)
                     /locus_tag="PLO35_00710"
     CDS             complement(4889..8980)
                     /locus_tag="PLO35_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78211.1"
                     /translation="MKRSRLFLTLVIILLLINAVFFTAWYGLGLKSRVKEYIEELAGN
                     AMGGKLRIKTYNLGDRQVYAEGVSFAASDSSMSFRVDNLRVRYDLFKFIFSGFKIKHL
                     LENVEINSPRLSLLIRPSKEPKKPAARFEIPDLSRYFKHLELKNGSVDLDLGIALSLG
                     GGELLLIKESLHSIEIVADNIQTTSLSLKAISNTGGKISLDGALDRGRVAKFTGQLTS
                     FVPSYIYHPALGYPTTELNLELSASQAHIKDPIELEAKALLWNTSAELLSAYPVQLPF
                     LSAELDKRSLKVALSTASVGSSRVGGQINLKDPLSRPRLDPSALNLRLDLAMIDKSLS
                     GIVSAAVQAEGSFAKPQAKITASSDQISVAGQQISSVRLTGQYEDELISFEALEAVWM
                     DHQIKAAGSLDPFALSLKAAVDGRPLAGARDIAVNMDADLELALYESLPEVKAEFREL
                     SLRKGNISLDKMSGSLSLFPATFNQTESYYINCDLNSADGQHLTAVGDVLDRSLIVDA
                     EFNTIAVSEIYPHELIYRYAPLVRGSVKGFMSNGTIAARTDLYLALKGEIDYATHINA
                     IGSYNLGTGDAALFLNSRKGSLNGAELEFDLAATMKDKQVNLSSLNLNDQIWLSGTLP
                     LDNIANATVSISVTDLSTSMVARFFPDLDLQLPEINDIDLSGTYNLEAPGIAVGSLNV
                     GSIQVNGIKPIAASIELRGPPAAISLSGEARGPQREIASLSAKLDLSKGLSIDVNGRV
                     HQLPVSEIVPDLPLELALSGDLRFAYGTEGSAHPGMLIGAKVSAPKLVIPDVIELEDL
                     RLEATQDQTKLTVDSLYVSSKGLAKLSGSGALDYNLLSGTFFDGNSRLQVKADGELFD
                     WLNNNVAMVLDAGGRSYLNLEIGTREEQFIITKGNIDIRSGYLQLKDQTEPITAINMA
                     ASVTDNRVSIDRASLIMGNGRMRFHNEFEEDSSNHFFVGFLDLGILKLAIDEPGILAN
                     IPYFTAPRTLSNIVMRGRDNPYLTVLGPFDDMKIKGDLYLSEGQALYPPNTDNLLSLV
                     YSFRGALSRPEAQTTASEPAPLPFSLDLMIHLRDNIRYVTYPASLDIQPGGLLHLVYD
                     GQTWSAREASFSSERGKIDFLGTVFQAEYLNVNILESQNLLIIDGSFFKRSADGTIIT
                     LRVTTNRDSSKPLMERLEFNLSSDNPRDRSITDILARLRYSQPTNELTGTQEQNLLQD
                     EALSLISDNLNASLLSPMFYPFENQVRRFLKLDSFSINVGFIQNLFTQYSNDPNQLAE
                     YVDMQHLMDDITQFSSTILLNNLSVSASKYLGRKLFLDYTLSLQEATDLQQRTRILVS
                     HDTSLRLFLPQQFKLGYTFKYEPQDERLSHELMLERSFRFWGI"
     gene            complement(8984..9655)
                     /locus_tag="PLO35_00715"
     CDS             complement(8984..9655)
                     /locus_tag="PLO35_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008983728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH78212.1"
                     /translation="MNILSAREISKSYTDSDQRIIVLEKASLEVEQGALISITGQSGC
                     GKSTLLHILGLLDEPDSGQVFIKGKQTSSQSPDAPAIRNQDIGFVFQFHYLIEDLCAR
                     ENVALPLLIAGQKLKDALSEADRLLELLSLGQRVSHYPNQLSGGEQQRVALARALITR
                     PAIVLADEPTGNLDPGNSLEVWNLIRKLNRELGQTFVVVTHDREQAKLADQTLELSKG
                     QVRLL"
     gene            complement(9669..10886)
                     /locus_tag="PLO35_00720"
     CDS             complement(9669..10886)
                     /locus_tag="PLO35_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH78213.1"
                     /translation="MNSTEKFFISRYLTAPKRNLLRFSFVFMVLGIVLSVGILSAGLN
                     LFEGYERALKSVLLDSFAHVRIERSDGKFLEAEQIQRIRKSLGPLDEISSITPSIGLN
                     LMAIDQANTRGCIFNAYETPAGQTPHYAKYVIKGKATLRDGQIIVGHYLADEFGVTLG
                     DTLRVLYPQLNRVTPMGIYSGEHLFEVAGIYRSGFYEFDRAMIIGTIADARSILFTDD
                     LYAYMDIKLRGSRMEDAYNLANQYSTLLGRDLSAYPVVGSGLMRTVAMQKWLIFIVFS
                     FLVLIAGINVISAVSTMIFDKKNEIAVLKTLGAPARTIRRVIDYQVLLVCFAAIILGQ
                     AFGALLSWMVVKQSFYRLKGDVYFIDRLEHYISLPNLLAVFAVAAVLVLICVWVPSRR
                     VDRLQIIDLLRNP"
     gene            complement(10900..11871)
                     /locus_tag="PLO35_00725"
     CDS             complement(10900..11871)
                     /locus_tag="PLO35_00725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH78214.1"
                     /translation="MHLSFVIPALNEQDSLQRLAQEIDQHSQPHSYEIIFVDDGSTDA
                     SFQIMKSLAAANPRVRIVKFRRNFGKAAALQNGFEIASGDVVFTMDADLQDDPVEIPA
                     FLKKLDEGFDLVSGWKRKRHDPWHKVLPSRLFNWVTARTFKLKLRDYNCGFKAYRLSL
                     VKELCLYGEMHRYIPALAHSLGYKVAEIPVQHRAREFGRSKYGIERYLRGFFDLLTVK
                     MVTHYSKSPLYLFGRIGLISTVLGGALTLYLSILKIFWGQPLSNRPLLFLGILLILGG
                     LQFISLGLISELIVNRVTSRQRLPISIESTVNVDRITGPAGDSAPGN"
     gene            complement(11880..12470)
                     /locus_tag="PLO35_00730"
     CDS             complement(11880..12470)
                     /locus_tag="PLO35_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOH78215.1"
                     /translation="MIKLSEKLRKSIHLSSLLIPFSYKYILGFSHRRLAFALLLAAFV
                     VSLTIEFYRLWQRSFRHTFYRLFGMILRKHELRDFTGATYLLFSSMLCVAFFEPTIAF
                     CSMAFLAIGDTAAALVGMSWGKRKFLGMSKSLEGSLGCFLSTLIFGAVFLDNPLLAVV
                     GALTATLAELWKIPVDDNVKIPLSSGLALTLASILI"
     gene            complement(12660..14225)
                     /locus_tag="PLO35_00735"
     CDS             complement(12660..14225)
                     /locus_tag="PLO35_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH78216.1"
                     /translation="MVDKWSLPQSPTASFVDQLKGSRPQLDLDSDPGLEALPDESLFA
                     NIDEVADRIRKAMYNNEPLVIFGHDDPDGITSTYILYQFFNSCGYQRHNYYIPNRNTT
                     SHGIQDDFVEYVRREGYKLVITVDNGIASYGGVEKLNAMGCDTLIVDHHLIQPDQLPR
                     AFAVLNPQLPQCAYPFKALAGVGVVLMLIRYLGRLLDHKIPASAYFWTAVGSIADKMP
                     MIGLNRLLVRHVINHWSEVNDPSIEFLLRNFNRVSSDMDVFNFIVYASRVIANGREDG
                     GQHMGMRFLLQMGDEKAVLFQTLEVQKKQWEGELNRVFGFLDTITAGFDGNAFIYYDD
                     EDVIPYNLLGTASTYVVSNFGIPTIMLKHHNGNMVCEGRCGDGFNMVEAFTRCKDHLL
                     QFGGHTRAAGFTMNAASYDAFLECYNDYLSQNQLREVIASLHQIDVLARPEDLSLANW
                     NYIQQLLPFGMQNPEPLLMLQSTTLLSLQQHYQIEYNSVNIPSSKELDIIFHWKSPGS
                     IRVMDYKLRPPGN"
     gene            complement(14219..15439)
                     /locus_tag="PLO35_00740"
     CDS             complement(14219..15439)
                     /locus_tag="PLO35_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78217.1"
                     /translation="MKRYIALTAVLAVVGLLGAGNSIFAYDGIPVRYYGNDVYGLSMG
                     DTGSADVFRYNSGFANPALHNSSNRTLLATGLLFGFTRYQSEDSAGNRRSFVDNSLDL
                     PYFSLSIPVKKHRLGFQFNSYASGLVNNEGTFTSGGFQVTERQKMDRYLYRGDLFYNY
                     KLGNYSLGLSGNYYFGHDVRSLDQEAGYAPYNTHEELSRSYKNPSLTLGAMASYQKFS
                     LGGYYSLGIVLKGESIRSSLHTTEAAIDYEYELPHQAGFGLSYLPLKEHKLNFDAYYE
                     AWNQVNPEMDDAYRFGIGWAYEPQPETRESYWSKIPLRGGLSWRHLPFMVEDSAVEEY
                     ALSLGLSFPLKRDINRLDLGFQVLNRGSLAQNKLSDLSFMMMLGLTGFDIIGKASDRT
                     APRDIPEKEELNEW"
     gene            complement(15556..16392)
                     /locus_tag="PLO35_00745"
     CDS             complement(15556..16392)
                     /locus_tag="PLO35_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOH78218.1"
                     /translation="MRLFETHAHLDLPEFDGDRDALIAKCFNSEIEYIINIGFNKETS
                     LASLELARKHLHVFATVGFHPHDATDFDAELIKRLAREKNVLAIGEIGLDFFRNLSPY
                     TVQRDVFANQAHLAVDYDLPVVVHNREAHQECYKILKQAEVKEAVFHCFSGDIIFAEQ
                     VLEAGWHISFTGTVTYANSKLDDVIRMMPLDRFMIETDCPYLPPHPYRGERNSPLHLH
                     LVAEKIAQIREMTPNQIAEHAYENAHRFFRVPPDPVKPQHHKASHKRNENKEKQQSGK
                     SG"
     gene            complement(17098..18030)
                     /locus_tag="PLO35_00750"
     CDS             complement(17098..18030)
                     /locus_tag="PLO35_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78219.1"
                     /translation="MKRFFAILVLTALLVSLSAIDDKFLYPLVHVKGAQATNIEVFDQ
                     QVLTRGSNQIWIYTIFNPWQPRIEMSYLSGAQIEDFDLVGDNYLYVVTREPANVIVPV
                     DSLNSYTRIYFTEHLPGDKITREGPTLYVADRFKGIDIINIGKGGLREIISTFSTNWG
                     IRDFVAIYPNIFALNDFGLVTVDINDLQFPVSRGQNYQINDARVMAKNGDTIWLGAGK
                     NLLGINIRDLKNPLLINQFRMPNEIVDLEVMDDRLFVALGRGGVKILNIKNPLQIDDL
                     NTINTPFTVHDVAISKDMIFLGLGRDGWVIYEYR"
     gene            complement(18033..18995)
                     /locus_tag="PLO35_00755"
     CDS             complement(18033..18995)
                     /locus_tag="PLO35_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOH78220.1"
                     /translation="MIASIDQLKQARTRLREQIHSVIVGQDRVIDEVLIGLFANGHML
                     IEGVPGLAKTLLISTIAKALNLSFGRIQFTPDLMPSDITGSDILVGSGQDREFRYIKG
                     PIFANIILADEINRTPPKTQAALLQAMQEYAVSSGTQTRPLPLPFIVMATQNPIEQEG
                     TYPLPEAQLDRFMFYVHIDYPDRDTELSIVKNTTSVLQPQITGLFSAEEILQVQAAVR
                     ALPLADHVLQYAVDLARKTRPQSEDASPDIRKWVSWGAGPRASQNLILAAKARAALDG
                     RVTPAEEDVRAVALPVLTHRVLLSFTAEAEGIKPRDIILSLTEK"
     gene            complement(19003..20007)
                     /locus_tag="PLO35_00760"
     CDS             complement(19003..20007)
                     /locus_tag="PLO35_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOH78221.1"
                     /translation="MNRKQLISLGFGLILGILLILLWSRLVETEELLFRFRSVLIRPV
                     LIALACYMLAYFIRSLRWYLLIGKSARVGMFRVWSYSLTGNFVNYLIPIRLGEITKAW
                     LLKERNQLPMPRSLSTIFIDKSFDTVGIFLALVLLPFLRVRFTGGMVILLGVLALIFA
                     VSLVLLLLAVKRKHNVIAVLQALFKWLPFRLRSKLNGYIELFVQGLNIFDHHWSRLLV
                     ALGLTILGVLLDGLYFWLIFSAFGLSYSFAMVLFGYTLINLSYALPQPPAQLGSNEWM
                     MIIIFSAGFGLTKADASAVMAFAHLLTAAVILISGVLSLSLNGSQIMRKIFKGEDLYE
                     "
     gene            complement(20117..20965)
                     /locus_tag="PLO35_00765"
     CDS             complement(20117..20965)
                     /locus_tag="PLO35_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOH78222.1"
                     /translation="MAIPIINNWESYFHHPDEGMGSSYERVILNDILLEIVKRYQIDS
                     VLESPAFGFTGLSGINLMELARQGIRISLEDHDASRLELIRDLWSQAQLSLDSRFNPD
                     YTKLDYPDKSFGMSFSFSALWFCQNLRAYLQELARVSSRCILISVPNRQGLGYQSQLQ
                     DYSPARYPELKPAHIDPPSIVSILSKTGWELKRSALFDCPPWPDIGMSKEDFLERKLG
                     IRLARKQVEAAKSNPPLCIMDHYLDKDPGFPERMRRLAFVEKHAPQAFKRIWAHHYYM
                     LFEPGG"
     gene            complement(20966..21976)
                     /locus_tag="PLO35_00770"
     CDS             complement(20966..21976)
                     /locus_tag="PLO35_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH78223.1"
                     /translation="MTRSVLITGITGLIGRSVLRAVLKLEESYRITALVRPGTSVERF
                     AEFVGRIELVELDLADISGLRKYLDASSFDVVMHIGALRGGRAYSRETFLKANLLCTQ
                     QLTEYCLKQGAILLFCSSVGVYGAIPQELPANDLSPRNPDNYYHLTKIESEKSIQQAV
                     LRGLRSAILRPAITYGPGDNGFPNMLVKMVDRRVFPLVNKRVWIHLCHIDMITKAFIW
                     LLTNDWEPGLALNVADREPVQLSALVHFISRQLSGKNYPSWILVDRNLFLLGEKIARK
                     LKNELWTSRFELISRSWFYEVRSTYELMELPPVFTIPSIQIVIDDYLRSHKSRGSRAT
                     QK"
     gene            complement(21973..22782)
                     /locus_tag="PLO35_00775"
     CDS             complement(21973..22782)
                     /locus_tag="PLO35_00775"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOH78224.1"
                     /translation="MIDTHCHIIPNIDDGSRDEQSSLRQLMEMDSGGISHAYLTSHYF
                     KGHYEYSREEYLAKLESLQRAVTAAGAKIKLLPGFEIFLQPNILQDVQAKNLCLGDSK
                     YILIESELNGLPPDFHINVYPLLRAGYRPILAHAERYVSIMKHPLEAQSLVERNLYLQ
                     VNSGSLLGQYGEKVKETAWLLIKYGWAHVLGSDDHTRGPYQSYFDALDAIEAEIDAHT
                     VKLLTETHPAMILEDKPIPYRYVYVDFPSHLSRHKRPHKSRSRWHKLFGKR"
     gene            complement(22827..23714)
                     /locus_tag="PLO35_00780"
     CDS             complement(22827..23714)
                     /locus_tag="PLO35_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOH78225.1"
                     /translation="MLLQKYFETRLDLIEQGLEQYLSFEGPTASELKEAMRFAVIPGG
                     KRWRPLLLIAIYEMLTGFKKNKGLADAVLAAVAIELCHNAALVHDDHPAVKNRSERRS
                     APSVHQKWDNAIAILTGDALYTLAFEVLGHISKPEKALAATRILASYTKSYGMIGGQM
                     VDVEMKRKVMKINTLRYIDMKKVGSLLQASADIACVLASVEENDRMVMNAYALNLGMA
                     YQMIEDIAADYNRGGDDLDFSDDYVPVSRGSYTGLLGFDKARNAVEKLLSESERMIKP
                     YANRDVLVEFIQMIQERLP"
     gene            complement(24033..24623)
                     /locus_tag="PLO35_00785"
     CDS             complement(24033..24623)
                     /locus_tag="PLO35_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOH78226.1"
                     /translation="MNWENSRMSERKLANPAVVGLAGFSMTTLVLQFHNVGWMGLGPV
                     IVLGLIFGGLAQLVAGFMEHKMGNNFGFCAFTAYGSFWIGLALILLMNHFHIFDSSTT
                     DIGYYLAAWMIFTFIMWIASWWIHTAMSLTFFTLFLGFLLLVLGHFVDPKWNMAAGYE
                     LMVCAGFAMYMMFANILNDLAGKTVLPFGSAWVKRT"
     gene            24916..26619
                     /gene="acsA"
                     /locus_tag="PLO35_00790"
                     /pseudo
     CDS             24916..26619
                     /gene="acsA"
                     /locus_tag="PLO35_00790"
                     /inference="COORDINATES: protein motif:HMM:NF003313.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            26751..26999
                     /locus_tag="PLO35_00795"
     CDS             26751..26999
                     /locus_tag="PLO35_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH78227.1"
                     /translation="MEEIRDVVLEYVRNEYMEDQDEELTYDTALISGGFVDSFSMVSL
                     KRFLETRYKISLPDNRATPEAFDTVNKITDLVMEFLGK"
     gene            27010..28257
                     /gene="kbl"
                     /locus_tag="PLO35_00800"
     CDS             27010..28257
                     /gene="kbl"
                     /locus_tag="PLO35_00800"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOH78228.1"
                     /translation="MAYSDKVRSLYTDQLKGLQDGGIFKIERYIHSVQDAEIEVEFPP
                     GAPLKKVINICANNYLGLSSHPDVVAAAHKGLDARGYGMSSVRFICGTQDIHKQLEQK
                     VTEFLGTEDTILFPSCMDANAGVFEAVLTDQDVMISDRLVHASLIDGIRLCKATTDSF
                     KHSDMAHLEEKLQLHSDKRVKLVITDGVYSMDGDTAKLDEMVALCEKYDAMILVDDSH
                     ASGFIGKTGRGTHEKCGVMGKIDIITTTFGKGLGGASGGCVSGRKELVELCRQKARPY
                     LFSNTIAPPVVAGVISVLDTLSKTTERRDKLEKNANWWRQHLLEAGFILQPGDTPIVP
                     VMLFNAKLSQDFARDLFAEGVYAIGFFFPVVPQGKARIRTQISAAHEMHHLEKALEAF
                     IKVGKKYDILGKTKAEVTAMYGN"
     gene            28548..29738
                     /locus_tag="PLO35_00805"
     CDS             28548..29738
                     /locus_tag="PLO35_00805"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOH78229.1"
                     /translation="MSKKFRSEIRKQLDAIVASGMYKKERVLATPQSPEIALTNGHKV
                     INFCANNYLGLSNHPEVIKASQKAMEKWGFGLSSVRFICGTQEIHIDLEKAVSDFLGM
                     EDTILYSSCFDANGGVFEPLLTEESAIISDALNHASIIDGVRLCKAQRFRYKHSDMNE
                     LEDILRQSSELKFRLIVTDGVFSMDGDIALLPQICDLAEKYDALVMVDDSHATGYIGT
                     TGRGTVEHCGVQGRVDIISTTFGKALGGASGGCISGSKEIIDFLRQKSRPYLFSNTLA
                     PGIVGGVLKVMEMISASTNFRDHVITNANYFRRGMIEAGFDILASDTAIVPVMIYQEP
                     LAVQMADRLLKEGIYVIGFTFPVVPVGQARIRVQLSAAHSLYHLDRAIHSFVKIGKEL
                     QVLK"
     gene            complement(29886..30761)
                     /locus_tag="PLO35_00810"
     CDS             complement(29886..30761)
                     /locus_tag="PLO35_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78230.1"
                     /translation="MNKSQPGNKSYQGSEFERIFIACLAVVTAAILIYLTIAGPLYQK
                     QISYRTHPDVIAQVIAQDAVNMYFLAPILIIAAWGLFNRSRWARYWLISTPLFLIYYA
                     VSYGIGWEWMAPSYEGNSHFWFFYYLEVLIAALLMMIYGLHIFPAKQRFIVKKAQLRI
                     YSILLGLFMLMFGMMWMKEIFQLYAEGSTRGYEAAPTAFWLVRLFDLGFSIPLALLSI
                     YLLWTRPDTSFGLQMLMYGFFLTMSVVVNAMGLMMWLSKDPAYDPSGTVIFAVLMLLS
                     VVGYLFVIRGYLRKH"
     gene            complement(30758..31375)
                     /locus_tag="PLO35_00815"
     CDS             complement(30758..31375)
                     /locus_tag="PLO35_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HOH78231.1"
                     /translation="MSKLLFKLEARLGAILLRCLRASIRWEVRGQNPDSLRCIYFLWH
                     RDLLILATQRIDSGAAVMVSSSKDGELIAGPLKYLGYTAVRGSSSRQGSSALKEMIRL
                     SRTHTLAITPDGPKGPARSIHPGVFEIAILGGVPIIAVATEAKREWVVNSWDRFRIPK
                     PFSRVKVEYSQPFYLRSREDIALTESLIRAFIDETQAKLKGEFGS"
     gene            complement(31368..32504)
                     /gene="lpxB"
                     /locus_tag="PLO35_00820"
     CDS             complement(31368..32504)
                     /gene="lpxB"
                     /locus_tag="PLO35_00820"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOH78232.1"
                     /translation="MAGKTIFWLVGESSGDLHASLVMKSLNEQVPDLIHTGIGGPRMQ
                     ALGLRSLYPFKRFAVMGFVEVLKHVFFFWRVEQGIKRFLKRSRPDLVILVDYPGLNLR
                     IANIADHARIPVLYFICPQFWAWKHERVFKLKAMTRHVACILPFEKEMLDIHTINSTY
                     VGHPIAEEIKFELDRASFAAFYKLDPNKQWLGFMPGSRNSEIKRMLPVFLRTSARFEE
                     DKYEFLFSKARGVDHQLYMDIIGCHKGNKINIIDGYTYEMMKYSEFVISTSGTATLEC
                     AFIGTPVLIGYKANPISYQIGRRLVRVKRIGLPNIVLDQDLLPELVQDDLNPERIHSI
                     VSGILADPERMKAMRTELYRLKGMLSSPKPSVEMPKLIRTMLGV"
     gene            complement(32510..33079)
                     /locus_tag="PLO35_00825"
     CDS             complement(32510..33079)
                     /locus_tag="PLO35_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOH78233.1"
                     /translation="MINKKGLLLILMLTLMLLPELEASGIANRTVDWLRGYGLSPGLI
                     VFLISMLPIIELRGAIPVGILLFGMPWPEVVLISVIGNMVPIPLILMFMEKFFTLISR
                     WKLGKRFTDWLFARTRRKGKVIERFEAIGLTIFVGIPLPGTGGWTGAFAANIFGIRFW
                     NSLLYVFLGVLIAAVIVTSLSLTGQMVVG"
     gene            33201..33650
                     /locus_tag="PLO35_00830"
     CDS             33201..33650
                     /locus_tag="PLO35_00830"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOH78234.1"
                     /translation="MKEQDIHWMSIALEEARLAALEDEIPVGAVLVREGELVLRDHNR
                     TRQLANPLAHAEKLLVDKILLSKIKYLQDYSLYVTLEPCLMCAGALIWSRIGRLVIGA
                     RDPKAGAVGSIYNVLTDKSFNHHPEIVRGVLEAESSAILKGFFARKR"
     gene            33658..33747
                     /locus_tag="PLO35_00835"
     tRNA            33658..33747
                     /locus_tag="PLO35_00835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33692..33694,aa:Ser,seq:cga)
     gene            complement(33846..34718)
                     /locus_tag="PLO35_00840"
     CDS             complement(33846..34718)
                     /locus_tag="PLO35_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78235.1"
                     /translation="MKAVTWIISAILAILAILFGIMWMNSANKISVAEKKARDTQKLY
                     ENATNTLNDVQTSLESMDQDLLGSIAGADEIPGTTPEERRARLINNISNMRSQIETNK
                     KKIADMERQLSQVKGQRDGYLKTLNNLKALNAEKESMLAQLESQLAASEAAKDSAIQA
                     SQQEIAQREAQIREKQGIIENQNQDLNQVYYIADTRKNLIAAGVVDRKGGILGIGRVT
                     TVSSKIVTEKFTPMNLLDSQQIALDPSPKGYAVLSNHVAASYTLMKEGESWILKVTDP
                     ESFRKQKFLVLEKK"
     gene            34844..36241
                     /locus_tag="PLO35_00845"
     CDS             34844..36241
                     /locus_tag="PLO35_00845"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOH78236.1"
                     /translation="MAEYRPQIEAVTRHKQTVGLCFKDGRVLSFVLRPNDSFPVLLPS
                     GESSGEPLWNQLRHAQVQRIWLDPSDRILYLEIRQTDIYQEVRDWQLIAELCPPRPNI
                     ILCEIQNGKPVVVDALHKYSLADNPARQILPRLEYQAPQTSWSPSQTDLRPPFTLKVP
                     ESADELRFDDVNSYFLAYYTHVIVAGEERERIRRQTAFWKKAIDKLEKKLAKQRLELL
                     SAEAMETWKLYAEVIKYNLGQINKGQTSLESVNYYDPNMASVLVPLREDLSPAQNMDL
                     YLKRYKKARTGLEVITRHIADGETELMHLQGVLARVEAGEELDFIVDGPKSSKSGHEK
                     LSILDKLLRIRISEEFEIVIGRKASENDFISTQLGRPHDWWFHTRIYRGAHVLLRCLK
                     KTDPPPELKSLCCRLAAWYSKARSSSNVPVDFTQVRYLRKPRRSAPGFITYTDHQTAF
                     VEPLETRKAREELGL"
     gene            36306..37055
                     /gene="recO"
                     /locus_tag="PLO35_00850"
     CDS             36306..37055
                     /gene="recO"
                     /locus_tag="PLO35_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOH78237.1"
                     /translation="MKGRVKDISIILKLSHYGESSLIAQAICRNLGSISFLAKGLRQK
                     PSSPPLQPLWGYELTLYEPLETGLYLFCEASPVTERSYPDPKRWSTALCGAELISHLV
                     VAADEHALYFDLLSGYLDYLVKTEGEPLPLFWRLFIRVMQLQGLKLELDLCASCGATT
                     SPAAYLKRDAGLICESCSRQDYSQDETNRFSPGARDLLTNLPSIGSCLGQLKLSRPIV
                     RELNEFFSHYYQGHTHQTLKLKSLSVLEQYY"
     gene            complement(37092..37595)
                     /locus_tag="PLO35_00855"
     CDS             complement(37092..37595)
                     /locus_tag="PLO35_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOH78238.1"
                     /translation="MALKENLILSIKKAMQGEMDSVTLYQNAANHSDDPEIKQFFHSR
                     REEERQHYNFLLKYYQEISSDLQPSDMSAELKSAEDLHPIFSENFIQRIGQDQYLFSA
                     ISTALLLEKDAIEHYHQCGLQTDSVTLKSFYDILEKWETRHYEDLLNIQKEAEVHYWQ
                     MNNFEPF"
BASE COUNT         8796 a        10013 c        10297 g         8581 t
ORIGIN      
        1 ctcagcaccg ccgctccatt cacatcgcgc cgcgatctca cctaccgcag gtatctcaca
       61 tgcgcagcat ctcacagcga agctctcaca gcgaagcatc tcaccatttc cgcttgacac
      121 cctgcctgct cccgttcagt ttggctttat aatcaaaacg aggtagccaa tgaagcgcgc
      181 cgtcctgatc tgtgtactga tcaccataag catttgcctc tgggcgggag ccaaaaaacc
      241 cagagcttcc aagcgccccg cctgggtgga tgatcccagc cagctttatc agccagagaa
      301 atatctcagc actgtgggga tagggaccag ccgttccgat gctgaagaaa acgccagtgc
      361 caatctggcc gccatcttgc tcacagaggt cacggcgtcc ggcgaggagg tggaccgtta
      421 tctgcaaatc accagcggcg ggcaaactca aaccagttca gtatctcagg tcagcacgtc
      481 gatccgggcc caggtggacc aggccctcgt caagctcaaa tttggcaaga gctggaaaga
      541 cgagcaagcc agacactaca ccgttgctta tcttgaaagg actgagacgg cggatatcta
      601 tctgggaatg ctggcagaca acaatgcccg cgtgatgagc tatctgagag cggtgctgga
      661 cgtgacggat ccctggaaat accacgcgtg gctaaacgcc gcgtggacca tcgatcagca
      721 cagcgctgcg ctcttgtctc agctggctgt aattgctccc aaggagcgca gtgcctacca
      781 ggccccctac gactcggaag tgctgaaatt ccaggccacc gatgccgccc gcaagctgca
      841 gttcagattt gagctcaaga ctccggaagc cgaagagctg cgccccaccc tggaaaccct
      901 tctcatttca atgggctttg tgatcgatcc ctatgctgcc aatctgctga gctgcgagct
      961 aaagctggaa ccggtagacc agctttcgga gcaaaagttt ttccgctatc tgctcagcct
     1021 ggccatcatc gataaagacg gcacccagat catcggatac agcgatagcg gacgggaagg
     1081 gcatcttatt gaatctcaag ccagggccgg agcggtcagc acagcggccg caaagctcga
     1141 aacggagttc cggaagacgc tgctggatta tatcgatacc ctggcgcgcc ggtagattaa
     1201 acttccgata aaatccatca attcatcgat tcccggcttt cacagtcttc aggaatgacg
     1261 gcctgctgcc aggtcctctg ttattttgtg tttggctaaa acctcctgat aggatatctt
     1321 gttattgacg tttgacagcg atcaagattt ttaaaataaa caaccagagg ataccatgct
     1381 cagacatatc tctctcatca tagccgttct ggcactggcc agcggcactc tcaacgctca
     1441 gttttccgcc tatgtggaac tcagtggagc gatcacaggc tacaacgacg cccaaagccc
     1501 caacaacgcc accacggacc gcttctcatt catcgacgat ctggaatccc ccgccatcgt
     1561 gcccagcggc agacttcaca tccgctggaa cgtcgctccc aagcatcagg tggggctgtt
     1621 gatgactcct ctcaccgtca acgcggatgg ccagtttgac cgctccatca cttatgaggg
     1681 cgagaccttt cttcccggtg aggatatcga ggccgtttac accttcaatt cctaccgcct
     1741 ccagtaccgc tatatgtttc ccaagctcaa ggtattgcgt tctttgggtt tatctatcaa
     1801 agtgcgggat gccgtgatct ctctgaagag cgacaccaaa tatgccgaga agacggacct
     1861 gggcgtggtg cccttgatca gcttcgacct gggccatcgc tttaacgaca agtgggagat
     1921 caactttgaa ggagaagccc tggcctccaa gtttggccgc gccgaggacg ttcagctctc
     1981 ggtggattac cgcgtcaacg aaagcaacga aatccgcttg ggatatcgcc tcctggaagg
     2041 cggctcggat atcgacgaag tatatacctt cgcgctcttc cactatccca ctatcggctg
     2101 gtccatggag ttctaaactc tttccagacc cttaacgcgc ccgcttttca ccaggcgggc
     2161 gctacttttt tgatcttcag ccttgacatc aaagaggata tggccaagat gctccctgag
     2221 gtttttatga cacatttctt gaaagttttg atcgtggacg ccgccacagg attctatcgg
     2281 atccagcgtt atccgctggg cgcattcttt ggtccggtgg acctggggct gcatctggct
     2341 ggcaaatata acagcctctg catcggggcc gggattttcg ccggttccat cttcccaggc
     2401 tccaaccggc tggttttcac aggcttttct ccctgctggg gagggtttta catatcctcc
     2461 atgggaggcg cgggcctggt gtgggacgat ctgggaatca acctgctggc catcatcggc
     2521 aaggctcccc agcccaaaat cttggtgctc aaccgtgatc acggtgagga gatcgacctc
     2581 agcctggaaa ccattgatcc cgaagcaatc tggaacagcg gcgaccgggg agtatatgcc
     2641 atgctccatc acagctttga cggtttcaaa gaccgctatg aaaatccccc cagagtgctg
     2701 gctgtaggcc ctgccgccaa atataccgat atgggaggca tcgtnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnncgccac caacgcggac agcctcctgg ccttcaacta
     2881 tcagagcatc cactggacgg acgaagagcg caaagccttc cacgccaaac atatccggaa
     2941 tcactatctg aagcagttca acgaggaaac catcgtaccc cgccagtttt ccacctgcgg
     3001 agaaccctgc ccagccgtct gtaaaaagaa tcacggcatc tataaaaagg attacgagcc
     3061 ctatcagacc atgggtcctc tatgcgggat atttgatcag cgcgcggccg aactgcttaa
     3121 ccgggaggca gatacattgg gctttgacgc catcaacgcg ggaggtatgc tggcctggat
     3181 gatggagtgt ctggatgccg gcctgctgaa accggaagag ctgggtgtgg atgtcaaacc
     3241 ccgttggaac cttacagatt ttgacacggt gacagactcc ctccacaacg ctcaactggg
     3301 agtgcggctc ctgaatgcct gtgtgggacc ggaccgcagt gtgaacatca gtgagggagt
     3361 ccgcaaatat gcccgtcgcc tcgccagagt gaaaggacag gccatcatgg accgcctcgt
     3421 gatcaactcc tttgcccgca agggctggat ggtgccaaac cagtattgga cgccgggggc
     3481 gctggccccc atgccggcca tgggacgcta ctatatgtac tatggcccgc aatactttga
     3541 gccccgtcaa ctgggaaaaa tctgcgccga tcgcatgaag acggaactta tgctggacaa
     3601 tctgggcttt tgccgtttcc acagggcctg ggcggaagag atgcttcccg aaatcgtcga
     3661 ggccctctat ggcctgggag ccaaactgga gctcagcagc cacatcaccg cccgtcgcat
     3721 caactgccgc aacgcatctg tcttttggga atccagccgc aacatcgact ttacccgcag
     3781 cttcctgctc cgtcataagc ggcagggcag ctcagatccc agcctcgaaa catggctggc
     3841 ccgctttgag gccaatcctc acgaagccgc cctggactgg tggtatgaaa tccgcaaagg
     3901 catagatgaa agtctgagag agaattagcg tcggaataat ctcacccaat cctcacagcc
     3961 ctgattgcct tctttgcagc acccctgccc ctccgatggg ccacctgtgc caatgtaaag
     4021 gagaggcaaa ggagaggtaa aggtggtgca aaggaggctg aagaggaagg gttgaaagat
     4081 ggttgaggat aaaaagaaaa acccgctggg ttcagcgggt ataaggtctg tgcaatcagg
     4141 agcttatggc agccagtcgt ccttaacggt gggtttttcc ttggggctgg ggtccaccac
     4201 ttcatagacg gagttgttga ggctgtattt cacctccacg ccttccttgc caaattcctt
     4261 ggtagagacg gcggtgatga tcttgtcgtc ataggaatag tcgaacttga agtctggatt
     4321 gggaaagctg ccaatctcgt ccagggtgat ggggtagtct ccaaattcgt cttgtttacg
     4381 atagtcttcc attgccttaa tgatacggtc gaacacaggg atagtggatt caaacttttg
     4441 ttgggcaatg gtttcctttt ccttgtcgat cttcatctgc tgcatgccaa agataaagac
     4501 cagcaccaat cccaccagca tcaggatcat gataagctca atcaggctga ctttgcgggt
     4561 catttcgttg ctttcgatct cggagcttgt tttatggatc ggaggcttgg gcgcggctgg
     4621 cttgggcgcg ggtgctacag gagctggtgc tacaggagct ggcgctgggg cagccggttt
     4681 gggggcctcg ggagcttctg gtttggttat ggtgggagtt tcgatggtcc cggacgcggc
     4741 gtcttgtaag ggctcttctg gcaatggttt gtctaggaca tcaggtgtct caatggtcgg
     4801 attctcggta ttctcggcca tgttatcctc cacggaattt ttcatttagc tcaggatttt
     4861 aaagtggctc attttgtcaa aggatttatt agatgcccca gaagcggaag gagcgttcca
     4921 gcatcagttc atgagacaga cgttcgtcct gaggctcata tttgaaggtg tatcccagct
     4981 tgaactgctg aggcagaaag agccgcaagg aggtgtcgtg tgataccagg atacgggtgc
     5041 gctgctggag gtcggtggct tcctgcaggc tgagggtgta atccaaaaac agcttgcggc
     5101 ccaaatactt actggccgaa actgagaggt tgttgagcag aatcgtggag ctgaattgcg
     5161 tgatgtcgtc catcaggtgc tgcatatcca catactccgc caattgattg gggtcgtttg
     5221 agtattgagt gaaaaggttc tggataaaac cgacgttgat gctgaagcta tccagtttta
     5281 aaaaccgtct cacctgattc tcaaaaggat aaaacatggg cgaaagcagc gaggcgttga
     5341 ggttgtcgga gatcaggctg agggcttcat cctgcaggag gttttgctcc tgagtgccgg
     5401 ttaattcgtt ggtgggctgg ctgtaacgca atcttgccag gatgtcggtg atagatctgt
     5461 ctctggggtt atcgctgctg aggttgaatt ccagccgttc catgaggggc ttggaactat
     5521 cccggttggt ggtgacgcgg agcgtgatga tggtgccgtc agcgctgcgc ttgaagaagg
     5581 agccgtcgat gatcaagaga ttttgggatt ccaggatatt gacgttgaga tattcagcct
     5641 ggaacaccgt ccccaaaaag tctatcttgc cgcgttcgga gctgaagctg gcctctctgg
     5701 cagaccaggt ctgcccgtca tagaccaggt gcagcaggcc tcccggctgg atatcgaggc
     5761 tggcgggata agtcacataa cggatgttgt ctctcagatg gatcatcagg tccagactga
     5821 agggcagggg ggctggctca gacgcggtgg tctgggcttc cggtctggaa agagctccgc
     5881 ggaagctgta aacgaggctg agcaggttgt ccgtattggg aggatacaga gcctgtccct
     5941 ccgaaagata gagatcgcct ttgatcttca tgtcgtcaaa gggtcccaaa acggtaagat
     6001 agggattgtc tctgccccgc ataacgatgt tgctgagggt gcgcggagca gtgaagtagg
     6061 ggatgttggc caggatgccg ggttcgtcga tagccagctt caggatcccg aggtccagaa
     6121 atcccacaaa gaaatggttg ctgctgtctt cctcaaactc gttgtgaaag cgcatcctgc
     6181 cgttgcccat gataaggctg gcacgatcga tgctgacacg gttgtctgtc accgaagcgg
     6241 ccatgttgat ggctgtgatg ggttccgtct ggtctttgag ctgcagataa ccgctgcgga
     6301 tatcgatatt gcctttggtg ataatgaatt gctcttcccg ggtgccgatt tccagattga
     6361 ggtaagagcg tccaccggca tccaggacca tggccacgtt attgttcagc cagtcaaaca
     6421 gttcgccgtc ggctttgacc tgcagtcggc tgttgccgtc gaagaaagtc ccgctgagca
     6481 ggttatagtc cagggctcca gatccgctca gcttggccag gcctttggaa ctcacataca
     6541 agctgtcaac cgtgagcttg gtttggtcct gagtggcttc caggcgcagg tcttccagtt
     6601 cgatgacgtc tggaatcacc agtttcggcg cgctcacctt cgctccgatc aacatgcctg
     6661 gatgggcgga accttccgtg ccataagcaa aacgcaaatc tccgctgagg gccagttcca
     6721 aaggcagatc gggcactatt tcggaaacgg ggagctggtg cacccggccg ttgacatcaa
     6781 tgctcaaacc cttgctgagg tccagtttgg cactcaggct ggcgatctct ctctgcgggc
     6841 cgcgagcctc tccactcagg gaaatcgccg cgggcggtcc tctgagttcg atgctggccg
     6901 cgataggctt gatgccattg acctggatgc tccccacgtt cagggagccc actgcaatgc
     6961 ctggcgcctc cagattatag gtcccgctca ggtcaatgtc attgatctca ggcagttgca
     7021 ggtccagatc gggaaagaac cttgcgacca tggaagtgga aaggtcggtg acagagatac
     7081 tgacagtcgc gttggcgata ttgtccaaag gcagggtacc gctcagccag atctggtcgt
     7141 tgaggttcaa agaactcagg ttgacctgtt tatccttcat cgtggcggcc aggtcaaact
     7201 ccagttccgc tccattgagg ctgcctttgc ggctgtttag aaagagggcc gcgtctccgg
     7261 tgccgaggtt gtaggacccg atcgcgttga tatgggtggc gtagtcgatc tcacccttca
     7321 gggccagata gaggtctgtt ctggcagcga tagtgccgtt gctcataaag cctttcacac
     7381 tcccacgcac cagaggagcg tagcggtaga tcagctcatg gggatagatt tcactcaccg
     7441 cgatggtgtt gaattccgcg tccacgatca agctgcggtc cagcacgtct ccgacggcgg
     7501 tcagatgctg tccgtccgcg ctgttcagat cgcagtttat gtagtaactt tctgtctgat
     7561 tgaaggttgc ggggaaaagg ctcaagctgc cgctcatctt atccagagag atatttccct
     7621 tgcggaggct gagctctctg aattccgctt tgacttccgg aaggctctca tacaaggcca
     7681 gttccagatc ggcgtccata ttcacggcaa tgtctctggc ccctgccaga ggacgtccat
     7741 cgacggcggc tttgagggaa agggcaaagg ggtccaggct gcccgcggct ttgatctggt
     7801 ggtccatcca cacggcttcg agcgcttcaa agctgatcaa ttcatcctca tattggcctg
     7861 tcagccgaac agaggaaatc tgctgtcccg ccacgctgat ctggtcggaa ctggcggtaa
     7921 ttttggcctg gggtttggca aagcttcctt ccgcctgaac tgccgcgctc acgattccgg
     7981 agagggattt atcgatcatg gcgaggtcca gcctcaggtt tagcgcgctg ggatccagcc
     8041 ttggtctgga aagagggtct ttcagattga tctggcctcc caccctgctg ctgcctacgc
     8101 tggcagtcga taaggctacc ttgagcgaac gcttgtccag ctcagcactc aaaaaaggga
     8161 gttggaccgg gtaagcggag agcaactccg cgctcgtgtt ccaaaggaga gctttggcct
     8221 caagctcgat ggggtccttg atgtgggcct ggctggcact gagctccaga ttgagctccg
     8281 tcgtgggata ccccaaagct ggatggtaga tatagctggg aacaaaggat gttagctgcc
     8341 cggtgaattt ggcgaccctg cctctgtcca gagcgccgtc caggctgatt ttaccgcccg
     8401 tgttggatat cgccttcagg ctgagagaag tggtttggat gttatctgcc acgatttcaa
     8461 tgctgtgcag cgattccttg atcaggagca attctccccc ccccaagctg agcgcgatgc
     8521 cgagatcgag gtccacgctg ccgtttttga gctccagatg tttaaaatat cggctcagat
     8581 caggaatctc aaaccgggcg gcaggctttt tgggctcttt ggatggacgg atcaggaggc
     8641 tgagccgggg tgaattgatc tcgacattct ccagtaagtg cttgattttg aagccactga
     8701 agataaactt gaagaggtca taacgcaccc gcagattatc cacccggaag ctcatactgc
     8761 tgtcggaagc ggcaaagctc actccctcag catagacctg ccggtctccc agattatagg
     8821 tcttgatccg cagtttcccc cccatcgcgt ttccagccag ttcctcgatg tactccttca
     8881 ccctgctttt gagtcccaga ccataccagg ccgtgaagaa aacggcattg atcaggagca
     8941 ggatgattac cagagtgagg aacagacggg agcgtttcat agcttacagc agacgcacct
     9001 ggcctttgga aagctcaaga gtctggtcgg ccagcttggc ctgctcgcga tcgtgggtta
     9061 ctaccacaaa ggtttgcccc agctctcggt tgagttttct gatcaggttc cacacttcca
     9121 ggctgttgcc gggatcgaga tttccggtgg gctcgtcggc cagaacaatg gcgggccgtg
     9181 tgatcagggc ccgggccaaa gccacccgct gctgttcacc tccgctgagc tgattgggat
     9241 aatgactgac gcgctgaccg agagacagca gttccagcag ccggtccgct tcgctgaggg
     9301 cgtccttcag cttctggcca gcgatcagta agggcaaggc gacgttttca cgggcgcaga
     9361 ggtcctcgat cagatagtga aactgaaaca cgaaaccgat gtcctgattg cggatagccg
     9421 gggcgtcagg actctgggag ctggtctgtt tgcccttgat gaagacctgg ccgctatctg
     9481 gctcatccag caggcccagg atgtgcagca gggtactctt gccgcagccg gattggccgg
     9541 tgatgctgat cagcgcaccc tgctccacct ccaggctggc cttttccaac acaatgatcc
     9601 gctggtcgct gtctgtgtag cttttactga tctcgcgggc tgataatatg ttcaaaccaa
     9661 aaactccttc agggatttct aagcaggtcg ataatctgga gccggtccac ccgtcgggag
     9721 ggtacccaca cacagatcag caccaatacg gctgccacgg caaacacggc cagcaggttt
     9781 ggcagcgaga tatagtgctc caaccggtcg ataaaataca cgtctccttt gaggcgatag
     9841 aagctctgct tgaccaccat ccaggatagc agggctccaa aagcctggcc cagaataatc
     9901 gccgcaaaac agaccagcag cacctgatag tctatgaccc tgcggatcgt ccgggcagga
     9961 gcacccaggg ttttgagcac cgcgatctcg tttttcttgt caaagatcat ggtggaaaca
    10021 gcactgatca cattgattcc ggctatcagc accagaaagc tgaaaactat gaagatcagc
    10081 cacttctgca tggcgacggt gcgcatgaga ccgcttccca ccacgggata agcgctcagg
    10141 tcgcgaccca gcagggtgga atactggttg gccagattgt aggcatcctc catgcgactg
    10201 ccgcgcagtt tgatgtccat ataggcgtag aggtcgtcgg tgaaaaggat ggaccgcgcg
    10261 tccgcgatcg tgccgatgat catggcccgg tcaaattcat agaagcctga tctgtagatt
    10321 cccgccacct caaagaggtg ctctccggaa tagatgccca tcggcgtgac tctgttcaac
    10381 tgcgggtaca gcacccgcag cgtgtcaccc agcgtaacgc caaactcatc agcaagatag
    10441 tggcccacga tgatctgtcc gtcccggaga gtggccttgc ctttgatcac atacttggca
    10501 tagtggggag tctgcccggc gggagtctca taagcgttga agatacagcc gcgggtgtta
    10561 gcctgatcga ttgccatcag attgagcccg atcgagggag tgatggagct gatctcatcc
    10621 aggggaccga ggcttttcct gatccgctgg atctgctccg cttccagaaa cttaccgtcg
    10681 gagcgctcta tcctcacatg ggcaaaggaa tccagcagca cgcttttcag ggctcgctca
    10741 tagccttcaa agaggttgag cccagcgctc aggatgccca ccgaaagcac gattcccaac
    10801 accataaaca caaagctgaa gcgcagcaga ttgcgcttgg gagcggtcaa ataacgggag
    10861 ataaagaact tctcggtgct gttcacgtct gcctggtcct tagttgcctg gggctgaatc
    10921 tccggcgggg cctgtgatcc tatccacatt gacggtgctt tcgatcgaaa tcggcagtct
    10981 ttgccgcgaa gtgacgcggt ttacgatcag ctccgagatc aggcccagag agatgaactg
    11041 caggcctccc aggatcagca gaatgcccag aaagagcaga ggccggttgg acagcggctg
    11101 gccccaaaag atcttgagaa tagacagata cagcgtcaga gcccctccca gcacggtgga
    11161 gataagtcct atcctgccaa acagatagag cgggctcttg ctgtaatgag tgaccatctt
    11221 gacggtgagc aggtcaaaaa aaccgcgcag ataacgctcg atcccgtatt tggagcgtcc
    11281 aaactcccgg gccctgtgct ggaccggaat ttctgccacc ttgtaaccca gcgagtgggc
    11341 cagggcgggg atatagcgat gcatctcgcc atacaggcag agctctttga ccagcgagag
    11401 ccggtaggct ttgaagccgc agttgtagtc cctcagctta agcttgaaag tgcgggcagt
    11461 gacccaattg aacagcctgg agggcagcac cttatgccag ggatcatgac gcttgcgctt
    11521 ccaacccgag accagatcaa aaccttcatc cagcttcttg aggaaagcgg ggatctccac
    11581 cggatcatcc tgcagatcgg catccatggt aaagaccacg tctccggatg cgatctcaaa
    11641 gccgttctgc agagcagcgg ccttgccgaa attgcggcgg aacttcacaa tccgcaccct
    11701 cggattcgct gcggccagag atttcatgat ctgaaagctg gcgtccgtgg agccgtcgtc
    11761 cacaaagatg atctcatagc tgtggggctg gctgtgctga tctatttcct gggccaggcg
    11821 ctgcagggaa tcttgttcgt tcagggcggg aatcacaaaa gaaaggtgca taactcctct
    11881 tatatcaata tactggctaa agtgagcgcc aggccggagc tcaggggaat ctttacattg
    11941 tcgtccacgg ggattttcca aagctccgcc agggtggccg tcagagctcc caccactgcc
    12001 aaaaggggat tatccagaaa caccgctcca aagatcaggg tgctcaaaaa acaacccaga
    12061 ctgccttcca gggatttgga catccccaaa aacttgcgtt tgccccagct catacccacc
    12121 agggccgccg cggtatctcc aatggccaga aaggccatgg aacaaaaggc aatcgtcggt
    12181 tcaaagaagg ccacgcagag catcgaggag aagagcaggt aggtggcccc cgtaaagtcc
    12241 cgtagctcgt gttttctgag gatcatccca aacagccggt aaaaggtgtg gcggaaggac
    12301 ctctgccaca gccggtaaaa ctctatggtg aggctgacca cgaaggctgc cagcagcagc
    12361 gcaaaggcca gacggcgatg actgaagccc aggatatact tgtaggagaa gggaatcagc
    12421 agcgagctga gatggatgct cttgcgcagt ttttcgctca gcttgatcat gatagctctg
    12481 cccggccggc attggcttgc gggcctgttt cagcgcggtg tgaacccata acagggcttt
    12541 gggtggtttc gatcttcatc gtcccgctca tggctgagtc ctttatcttc ttttggccgt
    12601 gcggcttgct taccagggct ttcccgctct gccgcccacg ccgaaaatca atctttatct
    12661 caattacccg gtggcctgag cttgtaatcc atgaccctga tgctgccagg gctcttccag
    12721 tgaaaaatga tatccaactc tttggaagat gggatgttaa cactgttata ctcgatctga
    12781 tagtgctgct gaagggagag gagcgtggtg gactgcagca tcagcagcgg ctccggattc
    12841 tgcatgccga agggaagcaa ctgctggata taattccagt tggccagaga caaatcttcc
    12901 gggcgggcca aaacgtctat ttgatgcagg gaagcgatca cctcccgcag ctgattctgg
    12961 ctcagatagt cgttgtagca ctccagaaag gcgtcgtagg acgcggcgtt catcgtaaac
    13021 ccggccgctc tggtgtgacc gccaaactgg agcagatggt ctttgcagcg ggtgaaggct
    13081 tccaccatgt taaaaccgtc tccgcagcgg ccctcgcaga ccatattgcc gttgtggtgt
    13141 ttgagcatga tggtgggaat gccgaaattg gagaccacat aggtggacgc ggtgcccagc
    13201 aagttgtagg ggatcacgtc ctcgtcgtca taatagataa aggcgttgcc gtcaaaaccg
    13261 gcagtgatgg tgtccagaaa gccaaacacc cggttcagct ctccttccca ctgctttttc
    13321 tgcacttcca gagtctggaa gagcacggct ttctcgtcac ccatctgcag caggaaacgc
    13381 atccccatgt gctggccgcc gtcttcacgt ccattggcga tcacgcggga ggcgtaaacg
    13441 atgaaattga agacgtccat atcgctgctg acgcggttga aattgcgcag caaaaactct
    13501 atggaggggt cgttcacctc gctccagtga ttgatcacat ggcggacgag caggcggttc
    13561 agaccgatca tgggcatttt gtctgcaatg gagcccaccg ccgtccaaaa atatgccgag
    13621 gcggggattt tgtggtccag caggcggccc agataacgga tcagcatcag caccactccc
    13681 actcccgcca gcgctttgaa gggataagcg cactggggaa gctggggatt aagcaccgcg
    13741 aaagcccgcg gaagctggtc tggctggata agatggtggt ccacgatcaa tgtatcgcag
    13801 cccatcgcat tgagcttctc cactccgcca taggacgcga tgccgttatc taccgtgatc
    13861 acaagcttat agccttccct cctgacatac tccacaaagt catcctggat cccgtgggat
    13921 gtggtgttgc ggttgggaat atagtagttg tgtctctggt agccgcagga attgaagaat
    13981 tgatagagaa tataggtgct ggtgatgccg tcaggatcgt cgtggccaaa gatcacgagg
    14041 ggctcgttgt tgtacatggc cttgcggatc cggtccgcaa cctcgtcgat attggcaaac
    14101 aggctttcat ccggcagggc ctcgagaccg ggatcggaat caaggtccag ctggggacgc
    14161 gagcctttca actggtctac aaaagacgcc gtggggcttt gtggcaagct ccatttatct
    14221 accattcatt caactcctct ttttccggga tgtctctggg agcggtgcga tcgctggcct
    14281 tgccgatgat atcgaaaccg gtgaggccca gcatcatcat aaaggacaga tcggagagct
    14341 tgttttgggc cagagagccg cggttcagca cctggaaacc caggtccaga cggttgatgt
    14401 cgcgtttgag cggaaagctc agccccaggc tgagagcgta ttcttccacc gctgaatcct
    14461 caaccatgaa aggtaaatgc cgccaggaaa gtccaccccg cagagggatc ttggaccaat
    14521 agctttcccg ggtttcgggt tggggctcgt aagcccagcc gattccaaag cggtaggcgt
    14581 catccatctc aggattcacc tggttccagg cctcgtaata ggcatcaaaa ttgagcttgt
    14641 gctccttgag gggaagatag ctgaggccaa atccagcctg gtgaggcagt tcatattcat
    14701 aatctatggc cgcttcggtg gtgtgcagcg aggagcggat gctttcgcct ttgaggacga
    14761 tgcccaggct gtaatagccg cccagactga atttctgata gctggccatg gcccccaaag
    14821 tgagggaggg attcttgtaa gacctggaaa gctcttcatg cgtgttgtag ggcgcgtaac
    14881 cggcttcctg gtccaggctg cgcacatcgt gaccaaagta ataattaccg ctcaggccca
    14941 ggctgtaatt tcccagtttg tagttgtaaa acagatctcc gcgatagaga tagcggtcca
    15001 tcttctgccg ttcggtaacc tggaaaccgc cgctggtgaa ggtgccctcg ttgtttacca
    15061 atcccgatgc gtaggaattg aattgaaagc ccagacggtg ttttttgacc gggatactga
    15121 ggctgaagta tggcagatcc agcgaattat ccacaaagct gcgtcggttg ccagcactgt
    15181 cttcagactg gtaccgggtg aatccgaaga gcaagccggt ggccaaaagg gtgcggttgg
    15241 atgagttgtg aagggctgga ttggcaaaac ctgaattgta gcggaagacg tccgcggaac
    15301 cggtgtctcc catactgagg ccataaacgt cgttgccgta gtaacgtacc ggaattccgt
    15361 cataagcaaa gatggagttc ccggccccca gtaagcccac gacagccaaa actgccgtca
    15421 atgcgatgta tcgtttcatt ttatcccttc ggtccgcttg cgggaccgga ttcccatccg
    15481 gcgcgccgcg ttatggagcg gatcagcgtt attcttttgt gtcatggttc tttatgagct
    15541 cctctggcga tggacttaac cgctctttcc agactgctgc ttttctttgt tttcatttct
    15601 tttgtggctg gctttatgat gctgtggctt taccggatct ggcggtaccc tgaaaaagcg
    15661 gtgagcgttt tcataggcgt gttcggcgat ctggttggga gtcatctctc tgatctgggc
    15721 gattttctcc gctaccaaat gcaagtgcag cggtgagttg cgctctcctc gatagggatg
    15781 aggcggcagg tagggacaat ccgtttcgat catgaaacga tccagcggca tcatccggat
    15841 cacatcatcc agtttggaat tggcataggt gaccgtccct gtgaaggaga tatgccagcc
    15901 cgcttccaaa acctgctccg cgaagatgat atcgccggaa aaacagtgaa acaccgcttc
    15961 cttcacctct gcctgcttga ggattttgta gcactcctga tgagcctcgc ggttgtggac
    16021 caccaccgga aggtcataat ccacagccag atgggcctga ttggcaaaga catccctctg
    16081 caccgtgtac ggcgacagat tgcggaaaaa atccagcccg atctcaccta tggccagcac
    16141 attcttctcc cgcgccagac gcttgatcag ctcggcatca aaatccgtgg catcgtgggg
    16201 gtgaaatccc accgtggcaa agacatgcag atgcttcctg gccagctcaa gagaggccag
    16261 tgaagtctct ttgttaaatc caatgtttat tatgtattcg atctcgctgt taaagcactt
    16321 ggcgatcagc gcatcccgat ccccgtcaaa ctccggaaga tcaagatggg catgcgtttc
    16381 aaacagtctc atgtgcgcac ttttccccaa tattagtcat cagacaacac aatcaaaggg
    16441 cttgtatctt gtcaaagaga atttttagca ctcgtaaaaa tcaaggtcag aacaggccaa
    16501 aatccagtta tcagggggtg ctcagtacag actttcccca ggctttccac aacgctttgc
    16561 tgagtccttc ccgattcgca ttacggagtt attacggact cattacggac taaatccgta
    16621 atgagtccgt aatggtgtgc ggcaaggaag gagtaagagc ttggggagga taaggtatgg
    16681 gcatacatat aatgatatat ataaatgata taggtatata tagtcaattc attggatgca
    16741 atcagtaatc tcaagtagct gtcagtaaat gtataaggta gatcgcaacg ctaatctctg
    16801 agcttgagag attgagaagg agttcttgga tgccaaagtc ggcaggagtc agtgtattca
    16861 cgataactgc ttatgtggcg gggattttgc ggtcgaacca ggcccgcgtc agcctcctaa
    16921 aaggattgtc atccagcgcc gcgactgccg tataaagtcc gcgcctcatt aaacatagac
    16981 catgcgagtc tgatgcagga ggtatgggag ttctaaacga tattctgatc ctggctgcca
    17041 ggcatgaaaa ataaccctgt caaccctctc gtacccatca accctccagc ttcaagctta
    17101 gcgatattcg tatatcaccc agccatcgcg tcccaggccc agaaagatca tatctttact
    17161 gatggcgacg tcgtggactg tgaagggggt gttgatggtg ttgaggtcgt cgatctggag
    17221 cgggtttttg atgttgagga ttttaacgcc gccccggccc agggcgacaa agaggcggtc
    17281 atccattact tccaggtcca cgatttcgtt gggcatgcgg aattggttga tcaggagcgg
    17341 gtttttgagg tcgcggatat tgatgcccag caggtttttc ccggctccca gccagatcgt
    17401 gtctccgttt ttggccatca cgcgggcgtc gttgatctga tagttctggc ctctgctgac
    17461 ggggaactgc aggtcgttga tatccactgt caccaggcca aagtcgttga gggcaaagat
    17521 gttgggatag atggccacaa aatcacggat gccccagttg gtggaaaagg tggagatgat
    17581 ctctctgagg ccgcccttgc cgatattgat gatatcgatg cccttaaagc ggtcggccac
    17641 atagagggtg ggaccttcac gggtgatctt gtcgccgggc agatgttcgg tgaaatagat
    17701 ccgggtgtag gagtttagtg aatccaccgg cacgatcacg ttggccggtt cgcgggtgac
    17761 cacatagaga tagttatctc ccaccaggtc aaagtcttcg atctgggccc ctgagagata
    17821 ggacatctcg attctaggct gccagggatt gaaaattgtg tagatccaga tctggttgct
    17881 gccgcgggtg aggacctgct ggtcgaaaac ctcgatgttg gtggcctgag cgcctttgac
    17941 atgaacgagg ggatagagga atttatcgtc tatagccgac agagacacca gcagtgctgt
    18001 gaggaccagg atggcaaaga atcttttcat gattatttct ccgtgaggga caggatgatg
    18061 tccctgggtt tgatgccttc cgcttcggca gtgaaggata gcaggacgcg gtgagtgagc
    18121 accggtaaag ccacagcccg gacgtcttct tccgcgggag tgactcttcc gtccaaagcg
    18181 gccctggctt tggcggccag gatcaagttc tggctggccc tcggtccggc cccccagctt
    18241 acccatttgc ggatgtccgg gctggcgtcc tcgctttggg gacgggtctt gcgggccaga
    18301 tctacggcgt attgcagcac gtgatcggcc aggggcaaag ctctgacggc tgcctgtacc
    18361 tgcaggatct cttcagcgct gaagagtccc gtgatctgag gctgcagaac ggaagtggtg
    18421 tttttcacaa tactgagctc agtgtcccga tcgggataat cgatgtggac ataaaacatg
    18481 aagcggtcga gctgggcttc cggcaaggga taggtgcctt cctgctcgat ggggttttga
    18541 gtggccatca caatgaacgg cagtggcagc ggacgcgtct gagtaccgct gctgacggca
    18601 tattcctgca tggcctgcaa cagcgcggcc tgggttttgg gaggagtgcg gttgatctcg
    18661 tccgccagga tgatgttggc aaagatgggg cctttgatat agcgaaactc gcggtcctgt
    18721 ccgctgccca ccaggatgtc cgagccggtg atgtcggatg gcatcaggtc tggagtgaac
    18781 tggatgcgtc cgaagctgag gttgagagct ttggcgatgg tggagatcag cagcgttttg
    18841 gccaggccgg gcactccttc gatcagcatg tggccattgg caaagaggcc gatcagcact
    18901 tcgtcgatca ctctgtcctg gcccacgatc acgctgtgga tttgttcccg caggcgggtt
    18961 ctggcctgtt tcaattgatc tatactggcg atcatctgct cgctattcat agaggtcttc
    19021 tcctttgaag atcttgcgca tgatctggct gccgttcaag ctgagagaca gtacaccgct
    19081 gatcaggatc acagcggcag tcaaaagatg agcaaatgcc atcaccgctg aggcgtccgc
    19141 ttttgtcaac ccaaatcccg cggaaaagat aatgatcatc atccattcat tggaaccgag
    19201 ctgggcgggg ggctgtggaa gcgcgtagga aaggttgatc aaagtatagc caaacaacac
    19261 catcgcaaag gaatagctga gcccaaaagc ggagaagatg agccagaaat agagcccgtc
    19321 cagcagcact cccagaatgg tgagcccgag ggctaccagc agcctgctcc aatgatgatc
    19381 aaagatattg aggccctgca cgaagagctc gatatagccg ttgagcttgg agcgcagccg
    19441 gaagggcagc catttgaaga gggcctgcaa caccgcaatc acgttgtgtt tacgcttcac
    19501 ggccaacagc aacagcacca gtgacaccgc aaagatcaaa gccaggacgc ccagcaaaat
    19561 caccatcccc ccggtgaatc tgactcttag gaaaggcagc aggaccaacg ccagaaagat
    19621 gcccaccgtg tcgaagctct tgtctatgaa gatggtggaa aggctgcgcg gcatgggcag
    19681 ctggttgcgt tctttcaaca gccaggcctt ggtgatttct cccaggcgaa tgggaatgag
    19741 gtaattgacg aagttaccgg tgagactgta actccaaacc ctgaacatac ctacccttgc
    19801 ggactttccg atcaacaggt accagcgcag gctcctaatg aaataggcca gcatataaca
    19861 ggccagagcg atgagcacag gcctgatcaa gacgctccgg aagcgaaaca gtagttcctc
    19921 ggtttccacc agccgtgacc agagcaggat cagcaggatg cccaggatca gaccaaaacc
    19981 cagggagatg agctgtttcc tattcatagc ggggagctaa aagtgagagt tgagcaggga
    20041 ggattgaaac gggcagggct catggtctca tatctcatag ttgttcagtt ctttttgtct
    20101 cggtggttaa tcggtttcac ccgccgggtt caaagagcat gtaatagtga tgggcccaga
    20161 tccgtttgaa ggcttgagga gcgtgctttt ccacaaaagc gagccggcgc atgcgctcag
    20221 ggaaaccggg gtccttgtcc agatagtggt ccatgatgca gagtggcgga tttgatttgg
    20281 ctgcttccac ttgctttcgc gccaggcgga tacccagctt gcgctccagg aaatcctctt
    20341 tgctcatccc gatatccggc cagggaggac aatcgaacag agcgctgcgc ttcagttccc
    20401 agccggtttt ggagaggatt gaaacaatgg agggggggtc tatatgggca ggcttcagtt
    20461 ccggatacct ggcagggcta tagtcctgaa gctgagattg atagcccagg ccctggcggt
    20521 tggggacgct gatcaaaatg cacctggaac tcactctggc gagctcttgc agataagctc
    20581 tgaggttctg gcaaaaccag agggcagaaa aactgaagct catcccgaag gatttatcgg
    20641 gatagtccag cttggtgtaa tcaggattaa acctgctgtc gagggaaagc tgtgcctggc
    20701 tccaaaggtc tctaatcagt tccaggcggc tggcgtcgtg gtcttcaagg ctgatcctga
    20761 tgccctggcg ggcaagctcc atcaagttta ttccactcag tcccgtaaag ccgaaagcag
    20821 ggctctccag cacggaatcg atttggtagc gcttcacgat ttccagcagg atgtcgttga
    20881 ggattacccg ctcgtaggat gatcccatac cttcgtcggg atggtggaag taactctccc
    20941 agttgttgat aatgggtatg gccatctact tttgggtggc ccgcgagccg cggcttttat
    21001 ggctccgcag gtaatcgtcg atcacgatct ggatagaggg gatggtgaaa acggggggca
    21061 attccatcag ttcataggtg gagcgcacct cataaaacca gctgcgggag atgagctcaa
    21121 aacgactggt ccagagctcg tttttgagtt tgcgggcgat cttttctccc agcaggaaga
    21181 gattgcggtc caccaggatc caggatgggt agtttttccc actgagctgc cgggagataa
    21241 agtggaccaa cgcgcttagc tgcacgggtt cacggtccgc cacgttgagc gcgaggccgg
    21301 gttcccagtc gttggtcagc agccagatga aggccttggt gatcatgtcg atatggcaga
    21361 gatggatcca gacgcgtttg ttgacgagcg gaaacactct gcggtcgacc atcttgacca
    21421 gcatgtttgg aaagccgttg tcacccgggc cgtaagtgat ggcaggcctc aggattgcgg
    21481 acctcagacc tctgaggacg gcctgctgga tgcttttttc actttcgatc ttggtgagat
    21541 gataatagtt gtccggattg cggggagaga ggtcgttggc cggaagttcc tgagggatag
    21601 caccatagac acctaccgaa gagcaaaaca gcaagatggc cccctgcttc agacaatact
    21661 ctgtgagctg ttgcgtgcag agcagatttg ccttcaggaa agtctcgcgg gagtatgctc
    21721 tcccacccct cagagccccg atgtgcatta ctacgtcaaa agaactggca tccagatact
    21781 tacgcaagcc ggagatgtct gccaggtcca gctccacgag ctcgatgcgg ccgacaaatt
    21841 cggcaaagcg ttccacactg gtgccgggcc tcaccagagc agtgatccga tagctttctt
    21901 ccagcttcag gacagccctg aggacggacc tgccaatcag cccggtgata cctgtgataa
    21961 ggactgagcg ggtcatcgtt tgccaaacag cttgtgccag cggctgcggg atttatgggg
    22021 ccgtttgtgt ctgctcagat gagaaggaaa gtctacatag acatagcgat aggggatagg
    22081 cttgtcttcc aggatcatgg ccggatgggt ctcggtgagc agcttgacgg tgtgggcgtc
    22141 gatctcggcc tcgatggcat cgagagcgtc aaaataggat tgataaggtc cgcgggtgtg
    22201 gtcatcggag cccagaacgt gtgcccagcc gtatttgatc agcagccagg cggtctcttt
    22261 caccttttcg ccatactggc ccagcaggga accggagttc acctgaagat acagattgcg
    22321 ctccacaaga ctttgagctt caagcggatg cttcatgatg ctgacataac gctccgcatg
    22381 agccaaaatg ggccggtagc ctgctctcaa cagaggatag acattgatgt gaaaatctgg
    22441 cggcaagccg ttgagttcac tctcgatcag gatgtatttg gaatctccca gacagaggtt
    22501 ttttgcctgt acgtcctgca gaatattggg ctgcaaaaag atctcaaagc caggcagaag
    22561 cttgatcttt gctcccgcgg cagttacagc ccgctgcagt gattccagct tggccagata
    22621 ttcttcgcgg ctgtattcat agtggccctt gaaatagtgg gaagtcagat aggcgtgtga
    22681 aatcccgccg gagtccattt ccatcagctg acggagggag ctctgctcgt ctcttgagcc
    22741 gtcgtcgata ttggggataa tgtgacagtg agtatctatc atgagttata aagttgttgt
    22801 tttttaaggg gataggacaa atggcctcac ggcaggcgct cctggatcat ctggataaat
    22861 tccaccagga cgtcccgatt ggcatagggt ttgatcattc gctcggattc gcttagcagc
    22921 ttctccacgg cgtttcgggc cttgtcaaaa cccagcagcc cggtatagct accacggctc
    22981 acgggcacgt aatcatctga aaaatcaagg tcatcgccgc cgcggttgta gtccgccgcg
    23041 atgtcttcga tcatctgata ggccataccc agattgaggg cataggcgtt catcaccatg
    23101 cggtcgtttt cttcgacact ggccagcacg caggctatat cggcagaagc ttgcaggagg
    23161 gagcccacct tcttcatgtc tatatagcgt agggtgttga tcttcatcac tttgcgtttc
    23221 atctccacat ccaccatctg gcctccgatc atcccataac ttttggtgta ggatgccaga
    23281 atgcgggtag cggccaaagc tttctccggc ttggagatat gccccaacac ttcaaaggcc
    23341 agggtgtaga gcgcgtcacc agtgaggatg gcgatggcgt tgtcccattt ctgatggacg
    23401 gatggagcgc tgcgccgttc ggagcggttt ttgacggccg gatggtcgtc gtgcaccaaa
    23461 gcggcgttgt ggcagagttc gatggccacg gctgccagaa cggcatcggc caggccttta
    23521 ttttttttga agcccgtcag catctcgtag atggcgatca gcaagagcgg acgccagcgt
    23581 tttcctcctg gaataacggc aaaacgcatc gcctctttga gctcggaggc agtggggcct
    23641 tcaaagctca gatattgctc caaaccctgc tcgatcaaat ccagcctggt ctcaaagtac
    23701 ttttgtaata gcaacttata actccctgtg tcaaaattgg acaagataaa tccaacctag
    23761 ctatacataa tatagaggcc taaattgtca agttatttat gcggctgaaa ggattttatc
    23821 aggtcctcca aagcgctgct ccgcatcccc cgcatcccaa agatgatccg cactattacc
    23881 gttcctgtgg gttcccctca cacaaagttt accaaagcaa aggaaaggcg gttgatgatg
    23941 tatggtccag cctgactgca tcccgttctt cccgcaaagc aaaaaccccg cgccggaagg
    24001 gcacggggtt gtgagagctt ggaaaaggct tctcaggtcc ttttgaccca ggcagagcca
    24061 aagggtagca ccgtcttgcc agccaggtcg ttgaggatat tggcaaacat catatacatc
    24121 gcgaaacccg cgcacaccat cagttcgtaa ccggcagcca tgttccattt ggggtccaca
    24181 aagtgaccca gcaccaatag caggaagccc aaaaacaggg tgaagaaggt gagtgacata
    24241 gccgtgtgaa tccaccagga ggctatccac atgatgaatg tgaagatcat ccaggccgcc
    24301 agataatagc caatatcggt ggtggaggaa tcaaagatgt gaaagtggtt catcaacagg
    24361 atcagcgcca ggccgatcca gaagctcccg taggccgtga aggcgcagaa cccaaagtta
    24421 ttccccatct tgtgttccat aaagccagcc accaattggg ccagtccgcc aaagatcaat
    24481 cccaacacga tcacaggccc caaacccatc cagcccacat tgtgaaactg cagcaccaga
    24541 gtggtcattg aaaagcccgc cagccccact acggctggat tggccagttt tctttcgctc
    24601 atgcgagagt tctcccaatt cattttattg ttcaggagct ggcatcagat gtcccgccaa
    24661 gccgaaacgt gattcaccaa atttggcggc tattttcctg tcaagtgttt ataggccgaa
    24721 accgttgaca aagcatcaac ggagcgtgtt tcaggacttt gtggcaccgt tgccggttcc
    24781 gtaatgttca actggataaa gaaggcagaa tttgtcattg acaaaaatcc gcccggcctc
    24841 tttcctgtaa aaagaaacca tttgtgagtc cacggtattt cgcacactgg ataaaaaacg
    24901 aaaaacgagg taagtgtcat gcacaatatc ggatcatacg aagaccggat caacggtttt
    24961 gactggaaga tatcagaaga ggaactgggt tacaaacccg gagacgtgat caacatcggc
    25021 tggtattgct cagaccgcgt ctgtcagatg ggcaagggag ccaaaacggc cctcatctgg
    25081 gaaggtctgg gaggcaaatt caagagctac agctacgacg atatccgtca gctcagcaac
    25141 accatcggag tcttcctgcg caagcttggc atccagcctg aggagcgggt ctgcctgttt
    25201 ctggaccgca tcccggagct ctacatcggc tttatcggca tcctcaaaac gggggccatc
    25261 gctcagccgc tgttttccgc ttttggggag gaatcgctgc acgtgaggct ggaaaacgcc
    25321 gagacctcag ccatcatcac ccaaaagaaa caccttggca aagtccgcaa gatcgtgggc
    25381 agccttcccc acctcagaca catcattgtg gtggacgaca accagaaatc cccccttcag
    25441 gataaggaaa tcgccttcaa tatggacgaa gcggaccggg tggacagctt tgaagtctac
    25501 cccaccctgg cagagtctcc ttccgtgctg cactacactt ccggtaccac aggtatgccc
    25561 aagggcgtca agcatgtgca ttactccctt atctcccaat accttactgc caagtgggtg
    25621 ttggacctta aagacgatga cgtctattgg tgtaccgccg atcccggatg ggtaacaggc
    25681 acatcctacg gcatcatagg cgcctgggcc aatggcgtga ctcaatgtgt gaacgacatc
    25741 ggtttttccg cggaggcctg gtaccgcttc atagagaaac accgcatcag cgtgtggtat
    25801 tcagctccca cagccatccg ttctttgatg aaagccggcg acgaggtgat caaacagttt
    25861 gacctctcct gtctgcgcca tctggccagc gtgggtgagc ccctcaacgc cgaagcggtg
    25921 atctggggcc agaaagtctt tggtctgccc ttccttgata cctactggca gacggagacc
    25981 ggttccatga tgatcaccaa ctatcccggc atgaagatca agcccggctc catgggaaaa
    26041 cccttcccag gcatcaccgc caccgtgctg gacgccaata aatacgagcc tcttccagtt
    26101 ggtcaggccg gtctgatcgg cttcaaacct ggctggccct ccatgatgcg cacctactgg
    26161 aacaacgaag aggtctataa agccaagttt aaaaacggct ggtatatccc cggcgacaga
    26221 tcatatatag acggggacgg ttactactgg ttcatcggcc gcgatgacga tatcatcaac
    26281 accggcggcc atctggtgag ccccttcgag gtggaatcag cactcataga gcatcccgcc
    26341 gtggccgaat ctgccgtggt tgccaagccg gactttgtga atatggaagt ggtgaaagcc
    26401 tttgtctgcc tcaaacccgg ccacgaagca ggcgacgaga tggaactgga tatcatgaac
    26461 tttatccgca aaaaactctc tccgctggcc atgccgcagg agatcgagtt cgtagccagc
    26521 ttgcctaaga cccgcagcgg caagatcatg cgccgcatcc tcaaagccca ggaatggggt
    26581 gaggaaatcg gcgacacttc atcgcttgaa gacgactaaa ggtgagacgc ttcgcgggtg
    26641 agagctgttg cggtgtgagt ttgactgtga gagctgtcgc ggtgaggtag ccctgcccca
    26701 aaaccataaa actatacaac cttacaacat aacagaacaa ggagattaca atggaagaaa
    26761 tccgtgacgt cgtgctcgaa tacgtcagaa acgagtacat ggaagatcag gacgaagaac
    26821 ttacctacga cacagccctc atttcagggg gatttgtgga ttcattttcg atggtttcgc
    26881 tgaaacgctt tctggaaacc agatacaaaa tctcgctgcc ggacaatcgt gccactcccg
    26941 aggcgtttga caccgtgaac aagatcaccg atctggtgat ggaatttctg ggcaagtaag
    27001 aggcgcgaga tggcttacag cgacaaggtc cgctctcttt acaccgatca gcttaagggc
    27061 ttgcaggatg gcggtatctt caagatagag cgttacatcc actccgtcca ggatgccgag
    27121 atcgaagtgg agttccctcc cggagctcct ctcaaaaagg tgattaacat ctgcgccaac
    27181 aactatttgg gcctttccag ccatccggat gtggtggctg ccgctcacaa aggactggac
    27241 gcgcgcggtt atggtatgtc ctcagtgcgt ttcatctgcg gcactcagga catccataag
    27301 caactggaac agaaggttac ggagttcctc ggcaccgaag acaccatcct tttcccctcc
    27361 tgcatggacg ccaacgccgg agtgtttgag gctgtcctca ccgatcagga tgtgatgatc
    27421 tcagaccgtc tggtgcatgc ttccctgatc gacggtatcc gcctctgcaa agccaccacg
    27481 gattctttca agcactcgga tatggcccat ctggaagaaa agctgcagct ccactccgac
    27541 aaacgcgtca agctggtgat cacggacgga gtctattcca tggatggcga cacagccaaa
    27601 ctggatgaga tggtggccct gtgcgaaaag tatgacgcca tgatcctggt ggatgattct
    27661 cacgccagcg gtttcattgg caagaccggt cgcgggaccc atgagaagtg cggagtgatg
    27721 ggcaagatcg atatcatcac caccaccttt ggcaaaggcc tgggtggcgc ttccggaggc
    27781 tgtgtttcag gccgcaagga actggtggag ctctgccgtc aaaaagcccg cccctacctc
    27841 ttttcaaaca ccatcgcccc acctgttgtg gcaggcgtga tcagcgtttt ggacactctt
    27901 tccaagacca cggaaagacg cgacaagctt gaaaagaatg ccaactggtg gcgtcaacac
    27961 cttctggaag caggatttat cctccagcca ggagacactc ccatcgtacc ggtgatgctt
    28021 ttcaacgcca agctctctca ggattttgcc cgcgatctct ttgccgaagg cgtctatgcc
    28081 atcgggttct tcttccctgt ggtcccgcaa ggcaaagcca gaatccgcac ccagatctcg
    28141 gccgcccacg aaatgcacca cctcgagaaa gccctcgaag ccttcatcaa agtgggcaag
    28201 aaatacgaca tcctcggcaa gaccaaagcc gaagtcactg ctatgtacgg caactaaacc
    28261 cctaatatac aatcatctac agcgcctctg ccgcatccgg ccgaggcgct ttttttgatc
    28321 aattccatca atcacatcat tcttccctat acacccgccg tggagatacc gtgcgctccc
    28381 cggcgaaaaa cggcggagac gccacggcat ctctacggcg ggtgaatggg caagaaacag
    28441 gtgccgagcc acttttcgga gctcgtccca gcaattctat tgacaaaagc ccccctcagg
    28501 cggatttgga gcgtaaaatc agcttttacc acataaagag gtttcaaatg tccaagaaat
    28561 tccgcagtga gatcagaaaa cagcttgatg ccatcgtggc atccggcatg tataaaaaag
    28621 agcgggtgct ggccactccc cagagcccgg aaatcgcgct caccaatggc cacaaagtga
    28681 taaacttctg cgccaacaac tatttggggc tttccaacca ccccgaggtg atcaaagcca
    28741 gccagaaagc catggagaaa tggggatttg ggctctccag cgtgcggttt atctgcggca
    28801 cccaggaaat ccacatcgac ttggagaagg ccgtttcaga cttcctcggc atggaagaca
    28861 ccatcctcta ttcctcctgt ttcgacgcca acgggggagt gtttgaaccg ctgctcacgg
    28921 aagaaagcgc gatcatttca gacgccctca accacgcctc catcatcgac ggagtgaggc
    28981 tctgcaaggc ccagcgcttc cgctacaagc actcggacat gaatgagctg gaagatatcc
    29041 tgcgccaaag cagcgagctc aaattccggc tgatcgtgac cgacggcgtg ttttccatgg
    29101 acggagacat tgctctgctg cctcagatct gcgatctggc cgagaaatat gacgcgctgg
    29161 tgatggtgga tgattctcat gccactggct acattgggac cacaggaagg ggaacggtgg
    29221 agcattgcgg tgttcagggc cgcgtggata tcatcagcac caccttcgga aaagccctgg
    29281 gtggcgcatc cggaggctgc atttccggca gtaaggaaat catcgatttc ctgcgccaga
    29341 aatcgcgtcc ctacctgttt tccaatacct tagctcccgg gatcgtgggc ggcgtgctca
    29401 aagtgatgga gatgatcagt gccagcacca atttccgcga ccacgtgatc accaatgcca
    29461 actacttcag gcgcggcatg atcgaagcgg gctttgacat cctggcctcc gatacagcga
    29521 tcgtgccagt gatgatctat caggagcccc tggctgtgca aatggcggac cgtctgctca
    29581 aggaagggat ttatgtgatt ggttttacct tcccggtggt gcctgtgggg caggccagaa
    29641 tcagggtaca gctctcagcc gcccactctc tctatcacct ggaccgggcg atccactcct
    29701 ttgtgaagat aggtaaagag ctgcaggtgt tgaagtaaag acctcgctac tggaatggca
    29761 gacctcccct tttgccaagg cgcgatcagc gcctgcggtt tggctctccg tcatcgcttt
    29821 cgcttggtta gcagatagat atccacctac ggaaacgaga cgtttccggt tccataacaa
    29881 gtgtctcagt gcttgcggag atatcccctt atcacaaaca aataacccac cactgagagc
    29941 agcatcagca cggcaaagat caccgttccg gatgggtcgt aggcgggatc tttgctcagc
    30001 cacatcatca gtcccatggc gttcaccacc acgctcatcg tgaggaaaaa gccatacatc
    30061 agcatttgga gcccaaagct ggtgtcaggc ctggtccaca ggagatagat actcaagagc
    30121 gccaggggaa tactgaagcc cagatcaaac aacctcacca gccagaaagc cgtcggagcg
    30181 gcctcatagc ctctcgtgct gccttcggca taaagctgga agatttcctt catccacatc
    30241 atgccaaaca tcagcataaa gagccccaac aggatgctgt atattctcaa ctgggctttt
    30301 ttgacgatga agcgctgctt ggcgggaaag atatgcaggc catagatcat catcaagaga
    30361 gccgcgatca gcacttccag atagtagaag aaccagaagt gactgttgcc ctcatagctg
    30421 ggggccatcc attcccatcc gatgccgtaa ctgactgcgt agtagatcag aaacagcggt
    30481 gtggaaatca gccagtagcg tgcccaacgg ctccggttga agaggcccca ggcggctatg
    30541 atcagaatcg gggccagaaa atacatgttc accgcgtcct gggcaatcac ctgagcgatc
    30601 acgtccggat gggtgcgata gctgatctgc ttctgataca aaggcccggc tatagtgaga
    30661 taaatcagta tcgccgccgt gacgactgcc aggcaggcga taaagatccg ctcaaactcg
    30721 ctgccctgat agcttttgtt tccgggttgg gacttgttca tgatccaaat tctcctttca
    30781 gttttgcctg agtttcatct ataaaggccc gaataaggct ttctgtgagg gctatatcct
    30841 ctctgctcct gagataaaag ggttgggaat actccacctt gactcgtgag aagggctttg
    30901 gaatacgaaa gcgatcccag gaattgacca cccattcacg cttagcctct gtggctacgg
    30961 caattatggg cacgcctccc aggatggcaa tctcgaacac gccgggatgg atagacctgg
    31021 ccggaccctt cggcccatca ggagtgatgg ccagggtgtg ggtacggctt aatctgatca
    31081 tctctttaag ggcgctggag ccctgacggc tgctggaacc cctgactgca gtatatccca
    31141 ggtatttcag gggtccggct atcaactcac catccttgga ggaggatacc atcacggccg
    31201 ccccgctgtc gatcctttgg gtggccagaa tcagcaggtc ccgatgccag agaaagtaga
    31261 tacagcggag agaatcgggg ttctgacccc tgacctccca gcgaatgctg gccctcaaac
    31321 agcggagcag aatggccccc agcctggcct ccagtttgaa cagcagttta gacacccagc
    31381 atcgtcctga tcagtttcgg catttccacg gagggtttgg ggctgctgag catgcctttt
    31441 agcctgtata gttctgtcct catggctttc atcctctccg gatcagccag aatcccgctc
    31501 acgatggagt ggatcctttc aggattgaga tcatcctgca ccagctccgg caggaggtcc
    31561 tggtccaaca cgatgttggg taaaccgatg cgtttgactc tcacgaggcg gcgaccgatc
    31621 tggtatgaaa tgggattggc tttataacct attaaaaccg gagttccgat gaaggcgcat
    31681 tccagagtgg cggtcccgga ggtgctgatc acaaattcgc tgtacttcat catctcatag
    31741 gtgtagccat ctatgatgtt gattttgttg cccttatggc atccaatgat gtccatatag
    31801 agctggtggt ctactccccg ggctttggag aagagaaact cgtatttgtc ttcttcaaag
    31861 cgagcgctgg tccgcaagaa gacaggcagc atgcgtttga tctcggaatt cctgcttcca
    31921 ggcataaagc ccagccactg cttgttggga tcgagtttat agaacgcggc aaaactggcc
    31981 ctgtccagct cgaatttgat ctcctcagca atggggtggc ccacataggt ggaattgatg
    32041 gtgtggatat ccagcatctc cttctcgaaa ggcaggatac aggccacgtg gcgcgtcatg
    32101 gccttgagct tgaagacccg ttcatgtttc caggcccaga actggggaca gatgaagtag
    32161 agcactggaa tcctggcgtg atccgcaatg ttggcgatcc tgagattcaa gcctggataa
    32221 tccaccagga tgaccagatc tggcctggag cgcttcagaa aacgcttgat gccctgctcc
    32281 actctccaaa agaagaagac gtgcttcagc acctccacaa agcccatcac ggcaaagcgc
    32341 ttgaagggat acagagacct cagccccaaa gcctgcatcc tgggaccgcc gatgcctgtg
    32401 tggatgaggt ctgggacttg ctcgttgagg cttttcatca ccaggctggc atgcaaatct
    32461 ccagagcttt ctcccaccag ccagaatatc gtctttcccg ccatggtctt cagcccacca
    32521 ccatctggcc ggtcaagctg agcgatgtca cgataacagc cgcaatcagc actcccaaaa
    32581 acacatagag cagagagttc caaaacctga tgccaaagat attggcggca aaggctcccg
    32641 tccagccacc tgttccaggt aggggaatcc ccacaaagat ggtgagcccg atggcttcga
    32701 agcgctctat taccttgcct tttcgacggg tcctggcaaa cagccagtcc gtgaagcgct
    32761 ttcccagctt ccatcgggag atcagggtaa aaaacttctc cataaacatc agaatcaagg
    32821 ggatgggcac catgtttccg atcacagaga taagcactac ctcaggccag ggcatcccaa
    32881 acagcaggat gccgacagga atggcgcctc tgagctctat gatcggcagc atcgagatca
    32941 gaaagacgat caggccaggg ctgaggccat agcccctcaa ccagtcaacg gtgcgattgg
    33001 cgatcccgga agcctctaat tcaggcaaca gcatcagcgt cagcatcagg ataagtagaa
    33061 gtcctttttt atttatcaca atttcctcat attcacattg ctaagtcccg tgtggcaaaa
    33121 ggggctgatt ttggcaagcg aaaaagcgcc atccctgctt tctggcttga caagcaagac
    33181 gtcacggtga atttgaccgc atgaaagaac aggatatcca ctggatgagc atagcgctcg
    33241 aagaagcccg tttggccgcg ctggaagatg aaattccggt gggagcggtg ttggtgagag
    33301 agggtgaact ggtacttaga gaccacaacc gcacccgcca gcttgccaac cctttggccc
    33361 acgcggagaa attgctcgtt gacaaaatcc tgctctcaaa aatcaagtac ctgcaggatt
    33421 acagcttgta tgtgaccctc gagccctgtc tgatgtgcgc cggggccctg atctggagcc
    33481 gaatcggccg cctcgtgatc ggggccagag atcccaaagc cggggcggtg ggttccatat
    33541 acaacgtcct gacggacaag agcttcaacc atcaccctga aatcgtcagg ggagtgctgg
    33601 aagccgaaag ttccgccatc ctgaaaggct ttttcgcgcg aaagcgttga tctgagtgga
    33661 gagttgccag agcggttgat tgggacggtc tcgaaaaccg ttgtgccttt acgggcaccc
    33721 agggttcgaa tccctgactc tccgccaccc ctgtagcgcc ggattccatt ccggcgcttt
    33781 tcttttttac aaagaaaaag cgccgtccga agacagcgct tatatctatg atcaagcagg
    33841 ataggctatt tcttctccag taccaggaac ttctgtttgc ggaagctttc gggatcggtc
    33901 accttgagta tccagctctc cccctctttc atgagtgtat aggaagccgc gacgtgattg
    33961 gacagcacgg cgtagccttt gggtgagggg tccaaagcaa tctgttgaga atccagaagg
    34021 ttcatgggag tgaatttttc agtgacaatc ttgctgctga cagtggtcac acggccaata
    34081 cccagaatac cgcccttgcg gtcaaccaca ccagcggcga tcaggttctt gcgggtgtca
    34141 gcgatataat agacctggtt caggtcttgg ttttgattct cgatgatgcc ctgtttttct
    34201 ctgatctgag cttcccgttg ggcgatttct tgctgagaag cctgaatagc gctgtccttt
    34261 gctgcttcgg acgcggcaag ctggctctca agctgagcga gcatgctttc tttttcagca
    34321 ttcaaagctt tgaggttgtt caaagtcttt aagtaaccat cgcgctgacc cttgacctga
    34381 ctgagctggc gttccatgtc ggcaattttc tttttgttgg tttcaatctg gctgcgcata
    34441 ttggagatat tattgatcaa gcgggcgcga cgctcttccg gagtggtgcc tgggatctca
    34501 tccgccccgg ctatgctgcc caacaaatcc tggtccatgc tctcaaggct ggtctgtaca
    34561 tcgttcaatg tatttgtagc attctcatat agtttttgtg tgtctcttgc tttcttctct
    34621 gccacactga tcttgtttgc ggaattcatc cacattatcc caaacaggat agccaatatg
    34681 gccaagatcg ctgaaattat ccaagttaca gctttcatgc ttgactcctc acggtgctta
    34741 ttctttatgg tactaattat tataagccgt attgctgtca agacaaaaaa acaatggagt
    34801 caatgctttg aattactcgc ttctaaaact ctggagccag acgatggctg agtatagacc
    34861 tcagatcgag gctgtaaccc gtcacaaaca gactgtgggc ctgtgtttca aggacggccg
    34921 ggtattgagc tttgtgctga ggcccaatga ttcctttccc gtcctgctgc ccagcggcga
    34981 gagcagcggt gagcccttat ggaaccagct gcgccatgcc caggtccaaa gaatctggct
    35041 ggacccgtcc gaccgtatcc tctatctgga gattaggcaa accgatattt atcaggaagt
    35101 ccgggactgg cagttgatag ctgaactatg tccgccccgt cccaatataa tcctatgcga
    35161 aatacaaaat ggcaaacccg ttgtggtgga cgcccttcat aaatacagcc tggctgacaa
    35221 tcccgcccgg cagatcttgc cgcgtctgga gtatcaagct cctcaaacca gctggagtcc
    35281 ctctcagaca gacctcagac cgccttttac tctgaaagtc ccggaaagcg cggatgagct
    35341 gcgctttgat gatgtcaatt catatttcct ggcctattac acccatgtga tcgtggctgg
    35401 agaagaaaga gagcggatca gacggcagac cgctttctgg aaaaaggcca tcgacaaact
    35461 ggagaaaaag cttgccaaac agcgcctgga actgctttca gccgaggcta tggaaacctg
    35521 gaaactctat gccgaagtga tcaaatacaa tcttggccag atcaacaaag ggcagaccag
    35581 tctggaatcc gtgaactact atgaccccaa tatggcctcc gtgcttgtcc cgttgcggga
    35641 ggacctcagc cccgctcaaa acatggacct gtatctgaaa cgttacaaga aagccaggac
    35701 cggcctggaa gtcataaccc gtcacattgc cgatggcgag actgagctca tgcacctgca
    35761 aggtgttttg gccagagtgg aagcagggga agagcttgat tttatcgttg atggccctaa
    35821 aagctccaaa tccggccacg agaagctgag tatcctggac aaactgctca ggatcaggat
    35881 cagcgaggag tttgagatcg tgatcggacg caaggcaagt gagaacgatt ttatcagcac
    35941 ccagcttggc aggcctcatg actggtggtt tcacacccgc atctaccgtg gcgcgcacgt
    36001 gcttttacgc tgtttgaaaa agactgaccc gccccctgaa ctcaagtcac tttgttgcag
    36061 gctcgcggcc tggtattcca aggccaggtc ctcatccaac gtgccggttg acttcactca
    36121 ggtccgttat ttacgcaaac cccgacgcag cgctcctggc ttcataacct ataccgacca
    36181 tcaaaccgcg tttgtagaac cgcttgaaac gcgcaaagca agagaggaac tgggattatg
    36241 acatacagat ttgcaagcgt cctggaaggc ttgattgccg tccggccgga gtggaaatca
    36301 tcctcatgaa aggccgcgta aaagatatct ccatcattct caagctcagc cactatggtg
    36361 agagcagcct gatcgctcag gccatctgcc gcaatctggg gagtatctca tttcttgcca
    36421 aaggcctgcg tcaaaaaccc agctctcccc ccctccagcc tctttgggga tatgagctca
    36481 cactgtatga gccgcttgaa acaggtctgt acctgttctg tgaagccagc cccgtcaccg
    36541 aacgaagcta tccagacccc aaacgctgga gcacggcact ctgtggagct gaattgatca
    36601 gccacctggt ggttgcagca gatgaacacg cgttgtattt tgatcttctc tccgggtatc
    36661 tggattatct ggtgaaaacg gaaggagagc cgctgccgct gttttggagg ctgtttatca
    36721 gagtgatgca gttgcaggga ctgaagttgg agctggacct gtgcgcctcc tgtggagcca
    36781 cgacaagtcc ggcagcctat ctgaaacggg atgccggatt gatctgcgaa agctgctcca
    36841 ggcaggatta ttcccaggat gaaacgaata gattcagccc cggggccaga gatctgctca
    36901 ccaatctgcc cagcataggc agctgtctgg gtcagctaaa actcagcagg ccaatcgtcc
    36961 gggaactgaa cgaatttttc agccactact atcagggcca tacgcaccaa acgctcaagc
    37021 tgaaaagcct gagcgttttg gagcaatact actgatcccg gccggggatt gccaccccgc
    37081 tccgtgcaag atcagaatgg ttcgaagttg ttcatctgcc aataatggac ctcggcttcc
    37141 ttttggatgt tcagcaggtc ttcataatgg cgggtttccc atttctccag aatgtcatag
    37201 aaggacttta gcgtgaccga atctgtctgc aggccacatt ggtgatagtg ctcgatggca
    37261 tccttttcca gcaggagggc ggtcgagatg gcggaaaaga gatattggtc ctggccgatg
    37321 cgctggatga aattctcaga gaaaatgggg tgcagatctt cagcgctttt gagctcggcg
    37381 ctcatatcgc tgggttgcag gtcgctggaa atctcttgat agtatttcag caggaagttg
    37441 taatgctgtc tttcttcttc acggcgggag tggaagaact gcttgatctc gggatcgtcg
    37501 ctatgattgg cggcgttctg atacaaggtc acactgtcca tctcgccttg cattgctttt
    37561 ttgattgata ggataaggtt ttctttcagg gccatggttt acctcgcggt tgatattcac
    37621 tttttagtga taatctaatc cgtcccggcc aaaaggaaaa ctgtaaatac tcaagacacg
    37681 gtctttggta gattgaagca atagaacaca gatgacacta atgatactga tctacacaga
    37741 ttccctgatg gaacacagat gacaggatga ctggctcgaa aggatag
//
LOCUS       DAOSFN010000005        37491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_868_length_37491_cov_8.594455, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000005 DAOSFN010000000
VERSION     DAOSFN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 37491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37491
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_868_length_37491_cov_8.594455"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..897
                     /locus_tag="PLO35_00860"
     CDS             40..897
                     /locus_tag="PLO35_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH78239.1"
                     /translation="MPNTGYQIRNLNKFYGKFQALNDVSLTLPEGRIIGLLGKNGAGK
                     STLMRCMLGFLSHQGTILLDDDPIVHRDPKVFEHVAFIPDVSGLDDRLTVQQTIDYIR
                     DVNPKWNEHIATKLLQISNLPMKKKVAKLSKGMKTKLYLLITLSLDVRYLLLDEPTLG
                     LDIAFRKEFFNTILGEFFDDSKGILISTHQVEEVEDILQEVIFIDDGRILLHEDVDKL
                     KERFRIVSLPQERSEEILAHKPLNVSRSLGFISAVLDSSLEFEGATYGRANLADIFLA
                     VAGGSNEAV"
     gene            884..1738
                     /locus_tag="PLO35_00865"
     CDS             884..1738
                     /locus_tag="PLO35_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78240.1"
                     /translation="MKQFKALVRKEWQTHRSTILIPLWFTGAVYVIGILGLMINFIRE
                     GRLTIVTGGMKVSAMEASVGLYGVSFAMLMMLGMVSIITAIILADGMINGAFKRKCEI
                     LHLSQPVSMLKVLGAKYALLVLGTFALSAALSLVNSFVLSGLLNIWMPVHTYYAMVAW
                     AQGSVGMLLSLLVVASYYWLSAAFFKRKSFFMGTLVILGIEIAIAVLNYTGKLGIPSL
                     MDYIAKLIGIQVHIEPHGMNALGLNLDDLIALQWKSVISWLTLQKLVLSAVFVAGGYW
                     FYSRRELT"
     gene            1873..3021
                     /locus_tag="PLO35_00870"
     CDS             1873..3021
                     /locus_tag="PLO35_00870"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH78241.1"
                     /translation="MIKRHIEHSIRALMHKKRAIIIYGARRTGKTTLLEQLAGDKQDA
                     LMVNGDFNVSHATFDFHTVEELEMRFGGYKYLFVDEAQRIVDIGIKLKAIIDAFPQLQ
                     VVATGSSSLDLSNFTTEPLTGRKFEFILSPFSTQELYDHDRINAVMDNLHLRLIYGNY
                     PEVYLNPSDAAEILAEVTGSYLFRDVLSYQEVRRPDLMRTLLKAIALQLGNQVSYAEL
                     GSIVGLDAKTVERYIYLMEQSFIIFRLGSFSRNIRTELKRSNKIFFWDNGIRNALINT
                     FDPLNNRQDVGALWENYFISERRKYMLNRRVGFDHYFWRTTQKQEIDLIEVAGQDLRA
                     FELKWSPKKKPSPPLTFSQNYPNAQMLVINRENYLPHLLNEGSFSKMG"
     gene            3086..3295
                     /gene="rpmE"
                     /locus_tag="PLO35_00875"
     CDS             3086..3295
                     /gene="rpmE"
                     /locus_tag="PLO35_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOH78242.1"
                     /translation="MKQGLHPKYEVVTVRCACGNTFETKSTKGNLQVEICNVCHPFYT
                     GKQKILDTAGRVEKFNKKYNLKADK"
     gene            3424..>3636
                     /locus_tag="PLO35_00880"
     CDS             3424..>3636
                     /locus_tag="PLO35_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOH78243.1"
                     /translation="MSKKEISVGGQAVIEGVMMRGPEALATAIRRKDGSIELYKQPFK
                     SVTTRIKWLGLPILRGFVNLIEMMKIG"
     assembly_gap    3637..3736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3737..4276
                     /locus_tag="PLO35_00885"
     CDS             <3737..4276
                     /locus_tag="PLO35_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOH78244.1"
                     /translation="FAGSIRIVFFVIYVWLISLMKDVKRLFRYHGAEHKNVNAYERNM
                     DLSIANIQSNTTIHPRCGTSFMFFVLLVSILVFSITDTLVSIYIIKGPVPVLIRLGYH
                     FLMVPIVSGLSYEVLKFSGKNLSHPLVKLMTVPGMALQRITTQPPDDDMIETALVAMK
                     AALDMDYSDHKVTVLDSLK"
     gene            4273..5340
                     /gene="prfA"
                     /locus_tag="PLO35_00890"
     CDS             4273..5340
                     /gene="prfA"
                     /locus_tag="PLO35_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOH78245.1"
                     /translation="MIPREKLNDIHKEQQELQTKVSDTALMSDARAYRDLMRRYNELS
                     SITACWNKFQELERHLEEARHLLATESDAEMSALAREEAEHLERELDSCESKLRELLI
                     PRDPNIDKNAIIEIRAGTGGEEAALFVADLYRMYSYYADMKGWKHQLIDLNETGLGGY
                     KEIIFQLSGEEAYGLMRFESGVHRVQRIPVTESGGRIHTSAVSVAVLPEAEEIDLEIN
                     EADLRIDVYRSSGHGGQSVNTTDSAVRITHVPTGIVVTCQDEKSQIKNKAKAMKVLRS
                     RLLDAEISRQEQEIASSRRAQVSTGDRSAKIRTYNFPQSRVTDHRINLTSYNLDGFLA
                     GNIEDFVQALRIAWQSEKVNE"
     gene            5343..6602
                     /locus_tag="PLO35_00895"
     CDS             5343..6602
                     /locus_tag="PLO35_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOH78246.1"
                     /translation="MSPAELIVIGTALLMLLAFSFVFSGFETGMISINQINLEHKARR
                     NRSAARLLAFIRQPDRFLGTTLIGNNIVNVLLASLSTYLVHRIDTPWLPAKYSAILIA
                     VIVLSFGEIIPKAIFRDHAETIVPRFFPILLFFYYLLKPLVYLVSLLNSGLRRFLKLD
                     ARGDFNYLTRDDLTFLLSQTQSDPSEQPQMEMIEDALEFREQVARNVMVPRMDIVAIP
                     GSATIEEAIAIAREEGFTRYPVYGQNLDDIIGVLIIYDILKRDCTKEMTAEQIVHEPL
                     FAPENTDLDVLLKEMQQKHRTMAIIVDAYGGTAGIVTMEDILEEIVGDIEDEYDAEEE
                     EDKDVEQISANTWLAQADVEIDHLADEHGIELPEGDYETIAGLILDKLERIPHQGQFI
                     SVPPYRLQVLQATDKKIIKVKIHKINN"
     gene            6611..>8250
                     /gene="ftcD"
                     /locus_tag="PLO35_00900"
     CDS             6611..>8250
                     /gene="ftcD"
                     /locus_tag="PLO35_00900"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOH78247.1"
                     /translation="MKLMECVPNFSEGRDQAVLDAIATEIRAVNNVVLLDVDPGADTN
                     RTVFTMAGDPEAVVEAAFLAIRKAAELIDMSKHHGAHPRMGATDVCPFIPISDMSMDE
                     CAEYARKLGKRVGEELGIPVYLYENAASKEEWRNLAVVRSGEYEALPQKAKDPAWKPD
                     FGPHVFNAKSGATAISAREFLIAYNINLNTRDKKKAHDIALSIRESGKPSRDAAGKLI
                     KDASGNKVMEPGLFTHCKAVGWFIDSYDRAQISINLTNYKVTPPQAVLDKVRELALQM
                     GVQVTGSELVGLIPKAALVEAGKHYLRKMNESTGLPEKMIMETAIQSMGLAELGPFEL
                     DKKVIEYAIARKDGLVKLRLDEFADLLSTDAPAPGGGSVAALCAAMSGALSAMVSNLT
                     VDKKGYESVQDKVRELAPIGQEIKLRALECIDKDTDAFYTMMDAMRLPKKTPEEISIR
                     DAAVEKSTQNAILIPFETLKISLEGLELAKQVAMVGNANALSDAGVAALTALAAAKAA
                     YYNIRINLAGITDDTFKQDILARSQDLIVLCEKIATEV"
     assembly_gap    8251..8350
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8576..9964
                     /locus_tag="PLO35_00905"
     CDS             8576..9964
                     /locus_tag="PLO35_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOH78248.1"
                     /translation="MYLDNLITENLSKLQIIASTREAIEGDWSELKKHLMMIPNRLPG
                     VWFYVRPDGNYYSLDKDLTGLNLRDRGYFASLFEGKPILGYPVFSRSSGKKSAVIAAP
                     VWTDGKVNGALGASIYLDDLHHRVNADLDLGIDYTWFAVNSEGLTMLDMDADFIFMNA
                     ITESPASLKSAIEQALQQESGEISYDLGEFKRQGRFQKLPNLDWWLIMAKKGLDEQSP
                     DPQKEFNLQSLISELQNSLDRCNDIAVKHMAVNGDTWTSEAAIRSVLSSIIADSPLIV
                     DVAWVDALGTMRYIEPPEFRNAEGVEIIKQPHVRETLVEGREVFSQSFISAEGFPAVV
                     IALPVKNAKAEILGAVSMLIRPELVVRRALSGMKLPADMEVCVIDNAGRIIFDDDVKE
                     IGLLLFEDPLYQEYESLLKLGRQIVEQPSGKGEYVFEARGTEEKTVKAAIWDSVGLYN
                     NQWRIIISCQQD"
     gene            complement(9961..11244)
                     /locus_tag="PLO35_00910"
     CDS             complement(9961..11244)
                     /locus_tag="PLO35_00910"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOH78249.1"
                     /translation="MNYQEFLDHIYQKYSGNVKLELDRMVGLLKDMGSPQQKLSGFHI
                     AGTNGKGSVCATLEALAMAHGLSTGLNTSPHLIDYTERFRIDGQELSFQRVLDTFLRY
                     EDIFEKWSASFFEISTAIAFAVFAEEQLDCSIIEVGLGGRLDATNLFTPDVTAITTIG
                     LDHVKTLGGTLELIAAEKAGIIKRGVPLVLGSIEPSPLHVIMKVAEGKGVPIYRWGLD
                     YLSIAGQDNIAGICFDYYYEDIGWKDLRANLMGEHQAVNLATALTCFIQYAAIKGFEV
                     SEPAVRNALMNINWRGRMQVLSIQPPIILDGAHNLHGIQALVKTLDKAFPGKKINFVI
                     SILADKDYRGMIELICSRAARIYVAQNQSDRAASAEDQLAEIGKHAVPATAYPSVAEA
                     YAQAVAELGPDDVLICGGSLYTVGEVLTASSASPA"
     gene            complement(11332..11994)
                     /locus_tag="PLO35_00915"
     CDS             complement(11332..11994)
                     /locus_tag="PLO35_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78250.1"
                     /translation="MLRNQKGISVYWILSAILFIGLIFILALPNFYNLDTEKNVDTCT
                     TNMKSIWVAATDYVRDFSTDFGGDLDVLRNTKKKQDAKNNYLTSITYCPEIQRDETNY
                     IVYGKYVEERLESGELKQNTGVIVICPDLEKHPKHFLPKTFYENMSPTVLQNYMVDDL
                     DYIDQQTKSNGPRKMQAVMKYISFWKNDREAFAKRKADSAYFKRLLYPDNFQSAPEPV
                     AE"
     gene            complement(12134..12661)
                     /locus_tag="PLO35_00920"
     CDS             complement(12134..12661)
                     /locus_tag="PLO35_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOH78251.1"
                     /translation="MAKTIPKTKGRTKRLFIGLAVLLLVFLVLVYNPVSVRLMTVGTA
                     IWHGLDPVLFYKLIRAESAFRSFAVSPKDAIGLGQIRESTAFYIHEKHKRGMLFVPYY
                     NLRLSAKYVKYLLKRYDGNWSLALAAYNWGETRVSKRIRHIKIEPGADYRQRFRDIPE
                     TYNYIVKIMGPPKKA"
     gene            complement(12700..13185)
                     /locus_tag="PLO35_00925"
     CDS             complement(12700..13185)
                     /locus_tag="PLO35_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOH78252.1"
                     /translation="MAVYSVNEIIEMAVQIERNGYAYYHEASKRKDLDARTLKLITTL
                     RDAELSHEKTFLNLRDDLDMSILELTGDWELVANYLKAIVDGRIFNDETSAIKRATEA
                     KDIMGVLDNAITFEKDTLLYFHAISDSITNERAKIILRKIINEEVSHVLMLNDYKKTI
                     A"
     gene            13918..14376
                     /locus_tag="PLO35_00930"
     CDS             13918..14376
                     /locus_tag="PLO35_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOH78253.1"
                     /translation="MNSVEQNERLVLDPNTIRELWSRTYNREGKPDWSHIFPYYHRNI
                     VFQDTIQRIEGIGNFIAMCKRLTRRTRQLKMEILAISQTDNVILFDWVMTMMFKKYPN
                     TPIYGATKLVLSEDGLIKEQRDYYDLWGDIFNNIPHFNKMYRRFLAKKFG"
     gene            14386..15357
                     /locus_tag="PLO35_00935"
     CDS             14386..15357
                     /locus_tag="PLO35_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH78254.1"
                     /translation="MNNKTRQYIRQYKWSNIFAMVRNNLRDSKLCRDDCQGRLVVITG
                     ATSGIGYAAARKYASRGAKLLCVNRNLAKSQRLQKEIQNEFGTECEFLIADLSKLSDI
                     HRVADELARLETPINVLIHNAGVYLTRREITSDGLEKVWVVHYLASFIINYRLMDKLR
                     AQDRARIIMVGSEGHRFAAWGLRLDDLNWEKRRYSGLGSYGSAKLAQLLAMIYFNEQF
                     RNSGVSINTMHPGAVKTETGQENGALYRWFKKHCFDRTLKSPLVSAEALYYLGVSKEV
                     DGVSGKFFNLSTMEDPAPPAMDRDVAGQLWEITLAMSGLLNNPEQEI"
     gene            15364..16971
                     /locus_tag="PLO35_00940"
     CDS             15364..16971
                     /locus_tag="PLO35_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOH78255.1"
                     /translation="MNKHSYDTIVVGGGMAGLTATAYLARAGQKVLLIEKNAELGGLV
                     SSFSSDGFLFDAGVRALESAGIILPMLAELQIKLEVVKSPVSLGIEDQILAIDNIASL
                     TRYRDLLKHLYPASAAEIDALIDTIRKIMKDMDVLYGVENPLFKDLVHDREFIFKKLL
                     PWLPRFLLTIGKINRMTYPVEDFLRSALSDDSLRNIISQHFFKGTPTFFAMSYFSLYL
                     DYFYPKGGVSKLAEALRDKILALGGEIRTATTVTRVAVGNQTLSDHLKEDYSYRKLIW
                     AADLKTLYRIADTRGLTARLQTRIGTVKTRMLQNRGTDSVFTIFLQVDEAPQSFAAVA
                     NGHFFYTSSRQGLRETHRKELQDLLHSFGAKSKPELLAWLDKFISLNTFEISIPVLKD
                     PDAAPPGKTGVIVSFLAEYELFRAVEVAGWLDEFVTLIEDRVIKVLAESVYPMLKDKV
                     LKRWSYSPLSIQKRVGSSEGAIVGWEFQAQMPVVNQIQNAARAVLTPLPSVYQAGQWA
                     YSPAGVPMAILTGKIAANRVIKTARKG"
     gene            complement(17002..17430)
                     /locus_tag="PLO35_00945"
     CDS             complement(17002..17430)
                     /locus_tag="PLO35_00945"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOH78256.1"
                     /translation="MKAKHKVLVLCTGNSCRSIIAEALINHLKGDTWQAWSAGVSPSR
                     VHPRSIKALRELGIDPSSLRSKSVEEFLDRDDLDLVITVCDHAREVCPVFLRPVKQIH
                     IGIADPTAQVWLSEEGSDEAFRKCRETIKRKIVDQLDSLI"
     gene            complement(17434..19899)
                     /locus_tag="PLO35_00950"
     CDS             complement(17434..19899)
                     /locus_tag="PLO35_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH78257.1"
                     /translation="MFLTDAQLKSELQRCEYCAEKPCKTACPADCSPADFIMAARGGK
                     PSDFKRAAGIILASNPLGGICGAVCPDYHCQAACSREKFDCAIQIPAVQATIVQKAKE
                     LGQMPEFIHPQPNGRKVAIIGAGPSGIGAAVTLIQLGYEVEVFEEQDIAGGQANLIPD
                     ARLDKDILSSDLEFMVNTFQVKISYDRKFASLDELKGRYDAIIQSVGLDQPIRLNIPG
                     AEAAFFGFDLLRNPDRYDLKGKRVALVGGAIAADMALTAVQKGAAHVEMITLESWLEM
                     PLTPVEREGLTQAGVQFTNRSRITEVIAENGVMTGIQIRPVTLPKGEKFHPAKIVDEL
                     GSGELFRPFDALFIAIGNRPSIREEGDGIWQAGDMSNGPSTVVEAVAAGKNVALRVDC
                     ALNAKAQPSFPKATKSIGPIFGWVKEPVPLKTDFFGREISAPFLLSAAPPTDGYDQML
                     KAYKAGWSGGIMKTAFDNVSIHIPGEYMVVFNDNTYGNCDNVSDHPLDRVCAEIKQLV
                     TEFPDRLTMASTGGPVSGNDEEDKQGWQANTRKLEASGVMGIEYSLSCPQGGDGTHGD
                     IVSQDAALTAKIVDWVMEISHPDIPKLFKLTGAVTAIYPIVKAIRDVLDRYPGKKAGI
                     TLANTFPTLAFRDKYPSDRPDTALWDEAMIVGMSGEGVLPISYLTIARAAKLGVSISG
                     NGGPMDYLGAANFLALGAETVQFCSIAEKYGVEIIDELRSGLSHYMQLKGIKSVKELI
                     GIAQPDPITDFMDLAPSHTISTLDKDLCVNCGNCTRCPYLAITLDDDRYPVIDRDKCV
                     GCSLCVKKCFTGALSMGPRPQAN"
     gene            20133..21254
                     /locus_tag="PLO35_00955"
     CDS             20133..21254
                     /locus_tag="PLO35_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH78258.1"
                     /translation="MQTDVVKYFMELVAIDSESKNERAMADRIMADLISLGAQVYEDD
                     AHKQTGGNAGSVYAWFDGDPSKTAILFCAHLDTVKPGNGIKALITDGRIHTDGTTILG
                     GDDKSGVVEIMAGIQRVLASGASHAPIEVLFTISEEIGLLGAKLFDKSKLKARMGYAL
                     DTHRVGELVLAAPSQNSIRICVNGVEAHAGVEPEKGINAIRVASEAIASMPMGRIDHE
                     TTCNIGVISGGIATNIVPNQVIIRGEARSHNMAKLEQVSSDIRHAVEAAVKRHHFSQG
                     SATYDWQCKREYDAFHIGEDEPVVKLAKAALAELGIECDAISGGGGSDANIINAAGIP
                     TIICGTGMNKVHTVHEDLLVSELERGTCFIESLINIHSR"
     gene            21263..22009
                     /gene="dapB"
                     /locus_tag="PLO35_00960"
     CDS             21263..22009
                     /gene="dapB"
                     /locus_tag="PLO35_00960"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOH78259.1"
                     /translation="MIKIGLIGYGKMGKMIAQLAESHGCQVVAIIDPKHPDQHAQINP
                     NVLAGADVCLEFTHPDSVMENIRQLAALRKHIVIGTTGWNEHLDEARNLAIRYQIGII
                     HGANFSIGMNLFNRLVAYASQLADKFPDYDVWGMEMHHNQKADSPSGTAIQLAETVIA
                     NHSRKTTAMYETLHRRAKPEELHFSSVRGGYVPGLHRIGFDSEADSIMLEHSVRDRSC
                     LAAGALQAAIWISERPGFFSFNDMMDSRLC"
     gene            22003..22788
                     /gene="dapF"
                     /locus_tag="PLO35_00965"
     CDS             22003..22788
                     /gene="dapF"
                     /locus_tag="PLO35_00965"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOH78260.1"
                     /translation="MLINFIKMQAQGNDFVMLNLLGNKELPLDPPGLAKAICDRRLGV
                     GADGLVLLRDDEIAAARMIIYNSDGSRAAMCGSALRCSAWLLAGQGKKNQLLISTDSG
                     PKSVEVEYTDKMVHANLGAPRLMEAKLELEGLEGSLVDVGNLHFISWWDALDDDPHLQ
                     YGYSLEHSPAYEQGLNAMFARVVSPQEIDLKIWENACGPTLACGTGATATVRAGLQKG
                     LLSGEVKVNMPGGTVSVKEDSAGDFILSGYVEDVFNGAYRWKI"
     gene            22776..23408
                     /locus_tag="PLO35_00970"
     CDS             22776..23408
                     /locus_tag="PLO35_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH78261.1"
                     /translation="MENLGLYLKQFREDQGIEYITVYSDIRLREEQVKLMEENRFFEL
                     GPYGVVKAMVFNYARYLGADLDAVMTELKVLVPEVTKSHHRPARPRKQKKIMLSTNFL
                     WGIGILVFVAILGGILFHAYNQGWLKTPQILKSAATDSLIVVPAEPPEPEQLAPDSTR
                     LRMRQLTQELNAPPKEEPVKPKAAVKAVKPDSTDYINSFMGDSPVNVETR"
     gene            24023..26827
                     /locus_tag="PLO35_00975"
     CDS             24023..26827
                     /locus_tag="PLO35_00975"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78262.1"
                     /translation="MKRSLFVIAAVILLIGTLTAQPNEFELLGQGQFSGKLGSYVSGD
                     TIFCVYNTATLASGTGSVILKASYDGGETWQSHTVAETVTGVGNPTVSWSPGELIVCY
                     VSGFERYIARSSNGGQNWQTASFGRSFESSPYVEKIDGEYHTFSLDLPYPQWDQDRYL
                     LSPESQSLRMPQYLTDMEEYPSSETSTFNGYDVLHGGVRTNNDLWIRQMGGGENNGWP
                     TFWGPVICGGTVRVYPDGGTNYPAVEIFRGGLIEHAPRLDPPLPNRRDAIIIGPAYYD
                     PNQIVLIEVDGSTYHGKLGTITPPIAQSANVYDIYPASPLGEPLYTNQYTEVDTVWAL
                     LGSGTCNNKMFFSHGPLWIKGNFSGHQSWQSGADIYIIGDITLSGTQPGLDPADNLND
                     SVSLLSEKNVIVKYGYRDPEDSLRYHLARSDDDPIYIYADISAAGLSPNLGMFTYEYQ
                     QPHPSVPSVNHLGQFWDQIDLHRRPYPQTAAQPWPANIDYPWYNPLWPEAKPYLERGK
                     LQIWGSVMQRRRGFLHRSLSDINNPTGIWNPAQNLFGGSSSPTFTNHLDPVLGIEMGT
                     QNYPGATGNGIGYKKDHHYNSRGIFGVTDQDIWTANESMCWNLGIHAGQLSAGPDNSF
                     VEDQFYPYQMMQLRKFRSKAYARRGNRFLHAFNERVLYLNGQNPVEIGYPLFEGGDVM
                     GIQWLNDDQALLHRYRYNAPTDQRYLEILDLDTQAHQALNIPGGEAPYPQDSPLSDIL
                     VIEDGTAFFGRYDPEFSLLRLWTLGQDNQFSPAGQWNLPASETGTPGPGSKLVLQSGT
                     GSMIGALLWWEYPADDTHPYPWGKLYGARIDAANTPNSDPVIPARIPTMNVWPNPSRT
                     MINLEIKGLASEALKAEVYNIRGQKVASLSGFDRKEDGSLSSTWNGSDSSSQRLPAGV
                     YLIRVKAGRDVKLVKRVSIY"
     gene            26982..28262
                     /locus_tag="PLO35_00980"
     CDS             26982..28262
                     /locus_tag="PLO35_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOH78263.1"
                     /translation="MSQQAHSFHEFMPADVWRWKMIEHQVDQVLALHDYEEIRLSVLQ
                     DYSVLYQGLTALMHQQQAEEAVRKTLNLCVPNDDISLISLRPEGTISVLHHTARVFQP
                     GEIHRFYYHGPMFRLDKNQRPMEFYQLGVELLGSETILSESEVISLGIKLCEALGLGD
                     VKLRINSYGCKQCRHKFFEEVENYLSSHQDDFCAKCYQSLRHNPFEDTHCDDPRCHHL
                     IKEGPKITDHLCERCKVDFARIKKIQANLGHRYTVDPFLYKNFSYYNETVFDFVLGNG
                     KQDLTIGGGGRYDDLSELITGKRIPAVGFYLNMDTIFAVLKQRGLFNPLAQAFSVYLC
                     AQSPDMEMMLLQIAHELHSLGVRTVLGIDHDPLERQIRKARGKECALLFAIREENIRD
                     GKILLHNLVKDHQEYIPLAQVVDAVLIARKALNR"
     gene            28287..28658
                     /locus_tag="PLO35_00985"
     CDS             28287..28658
                     /locus_tag="PLO35_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HOH78264.1"
                     /translation="MHSIVTSNAPAALGPYSQAVLKNDTLFVSGQLGFDLKDGSMPET
                     FEAQALLVFSHLRAILDAAGMSMANVVKVSVFLQDMNNFAKLNEIYARNFSAPFPARE
                     AIQVARLPKDGLIEISLIAMR"
     gene            28662..29213
                     /locus_tag="PLO35_00990"
     CDS             28662..29213
                     /locus_tag="PLO35_00990"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOH78265.1"
                     /translation="MSITTKTGDGGQTSLYSGQRVWKDELRVDAYGTLDELDAHIGDA
                     CHLIEDDELVQSLRGVQNLLYRAMGQLATLGEGFPYPICATDAEQLTSMIHGLEEQTP
                     ITGFVIPGSLPASARLDICRTIARRAERRVIALNRQEPVNPHLMGLVNRLSDFFYILA
                     RHLEDRAGVLTYKSKPEQPGCLS"
     gene            29326..30072
                     /gene="gpmA"
                     /locus_tag="PLO35_00995"
     CDS             29326..30072
                     /gene="gpmA"
                     /locus_tag="PLO35_00995"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOH78266.1"
                     /translation="MKQIVLMRHGESVWNKENLFTGWTDVDLSDKGLTEAHEAGKRLR
                     EAGFEFDLCYTSVLKRAIRTLWTALDEMDRMYLPILNDWRLNERHYGALQGLNKAETA
                     ARYGEDQVKIWRRSFDVPPPALDTSDERYPGFDPRYKGLSQQQLPTCESLKDTCARAL
                     PFWNEVIVPQLMSGTRMIISAHGNSLRAIVKHLDEISDEDIVGLNIPTGIPLVYEFDD
                     HLNVMNRYYLASDEELASAQTAVANQGKAK"
     gene            30126..30323
                     /locus_tag="PLO35_01000"
     CDS             30126..30323
                     /locus_tag="PLO35_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH78267.1"
                     /translation="MAKKPRENNMAVKIDKETCIGCGACVDVCPVSALSMEDGKAVCD
                     ENACIDCGACIGTCPVSAISE"
     gene            30475..33069
                     /locus_tag="PLO35_01005"
     CDS             30475..33069
                     /locus_tag="PLO35_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78268.1"
                     /translation="MKSTSMIIALLIFSVSLLATDVSGVQSGIWTLANSPYNLIGDVS
                     IPSGASLVINAGVTVYAMGNYRINAEGTLFANGTPADSIRFMNGQADPNALWVGIRLE
                     NENIPSSITYCYIENASYGVNSVNSPIEIAYCRFNKNQKGMQLYGIGSANPAGMDVHH
                     NLIERSIQNGILIPQNSNAHVHHNELRFNGTGTQYMAAIQLSNQSTGGENNPEINDNF
                     IHHNYKQGITAWDIVGANAIQPHIHHNIIEYNLTGIYLLNASGYVEENVIRSNFISGD
                     TNSGAGVMVAGATSAPYFERNQIYGNFTGFYLGTNAQPVLGDMSIYHAWAQGENQIYD
                     NIDESSTLHSVYTYSYTNSAIVIKAENNFWGSNDPAQINLGINDQLDSPSLPLVDYDP
                     WYWEPEPNTLLTGTIVYEGSQALHNYRIEIVGTVSEQVEHSFEVEYATPFSLAFEIDE
                     PYHVVAKADIVGQLKTLYGSPGGYWLTDTFQPGMTSDTAAFFLEDDLPPIAVVATEPI
                     QEDGHTLFPVYHKLWIYHWDHIDWLYEDGDFRYLKRHTRFHPDGNLEFSLPPNSVYDK
                     FQNLQPGDIWTRTSIYNDQGSIRTSQLRYVELDDGAAIDGSRFPVQLITQINMANVSL
                     MSQNLITGEDRGIVFIYDNNGNVTQMMSYLTNLLDTPLAWGSFINYDDFEYVTSPQFL
                     CYDPVRLQNEGIMELYWQAPASDHQHTWSQYRVYDNGILVATDSYGFPTTSFALATNT
                     DHTIRVAAYDGSVESELSPPLYIPAVANDDLVHPPLSLSVYPNPFNASQDLRIKLSSQ
                     IDAPGSLKIYNLRGQLLRTVPFSKETASELSWNGRDDSGRDCGTGIYLLRIQIPGMKA
                     IGRRIVKL"
     gene            33392..>37491
                     /locus_tag="PLO35_01010"
     CDS             33392..>37491
                     /locus_tag="PLO35_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH78269.1"
                     /translation="MQSSFMGKAVLIISVLLSFCLILSAQSRQIDLSTQSNQTLLKNN
                     SDLGFEVSYSISQLSLREVNTRAGSFDELSIAGYTHTNAIGEPKLPMQRQLIAVPLGA
                     EVRYSILSSSTRDLDAKDSQLRHRIIPAQEPVSKSADPSTLPFILKEEAYQKADYRSR
                     EVVRVEDLGFMRGIRLFAIDFYPVSYDPLQNSLTVLESAEIRIDFINPDLIGTAELLA
                     RTGSVEYDQLYASSIFNWNTDTRTSLVRYPTKMLILCPPNYTDEMQSFVDWKKQQGYN
                     VIVTTVGTGGTVANTTTAINTYMASVWSAATAQDPAPTYLLIVGDTSTSGDNIIANTG
                     VTSSHVTDLTYVRLQGTDYVPEMYYGRFSVSSATELTNIINKTLTFEKTQMTDLSYLG
                     KAVMIAGQDGTFGPTHGNGAINYGTTQYFNASNGVTSNTYLHNISGTSDAAIIANANE
                     GRGYINYTAHGSETSWADPTFSVSDVNSMINAGKYGVMVGNCCVTNAFGTGVCFGESI
                     IRKANAGGVAYIGGTNNTYWDEDYWWAVGAKGTANGTAPAYDATKLGAYDAMFHTHSE
                     AQTDWAQTTGDAILMGNLAVAQGASSRTNYYWEIYSIMGDPSLMPYFKVPSVNTATFP
                     ATILIGATSINITAAPYSRVGLSMGGTLYGTGIVPASGSLTLTITPFASTGTATLVIT
                     AQNKVTRIESITIAPNSGPYVNVTASNYNDSNNNQPEYNETGRFSPVFQNVGSVAATN
                     VSATLTCSTPGITITDGAHSIASLAAGASSTATNAYSFTIANNIANGTVAQFTITSTM
                     SGQTPWVYNFSLTINAPALAFGNITIQDPSGNNNGRLDPGETVTLVMPLNNTGLAASP
                     AGSATLSCSTPGITVNTGTASFAAISASGTASLSFSITAASSMTIGTVASLVFNATAG
                     SYTANKTENTAVGLILEDFETGNFSSYPWILGGTLPWTVANTGAHAGTYTAKSGAITH
                     SQSSTMETTRILSTSGNLSFWYKVSSESGYDYLKFYVDGVMQNQWAGTVDWTQASYTL
                     AAGTRVLKWEYMKDGSVDSGSNCAWVDDIIFPASTAPSNFYPPQNLAATPGNGFVNLA
                     WQAPQTGTPTGYKIFRNSALLTTVTGLSYTDNAVVNETTYSYYLKAAYSGGDSDPTAT
                     VTAMPTAIVPTSVILGTGTASTGTSEGAPINIWYKSLHGQSVYTSAELNAAGITGPRS
                     ITQIGFNITALPALAMPNFVVRIGHTTASNAASWTSTGLTQVWSSTSYLPTTTGWNMY
                     TLTTPFLWNGTGNIVIDTAFGLLANYNQSGTVQISTMTSGYIFGRSDTIDQTNLFTGT
                     STSTYRPNVKMVFQPNADGPSIAVSPSSLAFGEVAVGGSSVQQFTIL"
BASE COUNT         9024 a        10232 c         9762 g         8273 t
ORIGIN      
        1 aggtaattga catattagac caggcttatg gaggaacaga tgcctaacac cggttatcag
       61 atccgcaatc tcaacaagtt ctacggcaag tttcaagccc tcaacgatgt atccctcacg
      121 cttccagagg gcaggatcat cggccttttg ggcaaaaacg gggccggaaa atccaccctc
      181 atgcgctgca tgctgggctt cctttcccat caggggacta tcctgctgga cgacgatccc
      241 atcgttcacc gcgatcccaa agtgtttgag cacgtagcct ttatcccgga tgtgagcggc
      301 ctggatgacc ggctgacggt gcagcagacc atcgattaca tccgggacgt caatcccaaa
      361 tggaatgagc atatcgccac caagctgctg cagatcagca atctgcccat gaaaaagaag
      421 gtggccaagc tctccaaggg catgaagacc aagctctacc tactgatcac gctgtccctg
      481 gacgtacgtt atctgctatt ggatgagccc actctgggac tggatatcgc cttccgcaag
      541 gagtttttca acaccatcct gggcgagttt tttgacgaca gcaagggcat cctgatctcc
      601 actcaccagg tggaagaagt ggaagacatc ctgcaggaag tgatctttat cgacgatgga
      661 cgcatccttc tgcatgaaga tgtggacaaa ctcaaggagc gcttccggat agtgagcctg
      721 ccacaggaac gctcggaaga gattttggcc cacaagccgc tcaacgtttc aaggtctttg
      781 ggtttcatca gcgcggtgct ggattccagc ctcgaatttg agggggccac ctacggccgg
      841 gcaaatctgg cagacatctt tctggccgta gctggaggaa gtaatgaagc agtttaaagc
      901 actcgttcgc aaggaatggc agactcacag gagcactatc ctgatccccc tctggtttac
      961 cggtgcggta tatgtgatcg ggattttggg cctgatgatc aacttcatcc gggaaggccg
     1021 gctcacgatc gtgacgggtg gcatgaaggt gagtgccatg gaagcctcag tcggacttta
     1081 tggcgtctcg tttgccatgc tgatgatgct gggcatggtt tccatcatta cagcgattat
     1141 tctggcagat gggatgatca acggcgcttt caaacgcaag tgtgagattc tgcacctgtc
     1201 ccagcccgtt tctatgctca aagtgctggg ggccaaatat gctctgctgg tgttgggcac
     1261 ctttgcctta tccgcggccc tgagcctggt aaacagcttc gtgctgagcg ggttgctgaa
     1321 tatctggatg cctgtccaca cctactacgc catggtggcc tgggctcagg gcagtgtggg
     1381 aatgctactc agcctgttgg tggtggcatc ctactactgg ttgagtgccg ctttcttcaa
     1441 gcgcaagagc ttcttcatgg gcactttggt gatcctgggc atcgaaatcg cgatcgccgt
     1501 actgaactac accggcaaac tgggcatccc ctcattgatg gactatatcg ccaagctgat
     1561 cggcatccag gtccacatcg aaccgcatgg aatgaacgcg ctgggcctga atctggacga
     1621 cctcatcgct ctgcagtgga agagtgtcat atcctggctc actctgcaga aactggtgct
     1681 gagcgctgtc tttgtggcgg gaggatactg gttttacagc cgccgggaac ttacttagaa
     1741 ccccactcca agcataaaca aacaggaacg tattcccgaa atatcatgat aaatcgggat
     1801 tatattcctg ttttcttatg tttcttcttg ccttaaattg catatgtgtt agcttgtatc
     1861 caggagaaag atatgataaa gcgccacata gagcatagta tccgagcttt gatgcacaaa
     1921 aaacgggcaa tcatcattta cggggcccga cgaactggaa aaaccaccct tcttgagcaa
     1981 ttagctgggg ataaacaaga cgccttaatg gtgaacggag acttcaacgt cagccacgcg
     2041 acctttgatt tccacaccgt ggaagagctt gagatgcgct ttggtggcta caaatatctg
     2101 tttgtcgatg aagctcagcg gattgttgac attggcatca agctgaaggc cataattgat
     2161 gcctttccac agctacaggt ggttgccact ggatcttcct ctctggacct gtccaatttc
     2221 accacagaac ctttgactgg ccgtaaattt gaatttatcc tcagcccctt ttccactcaa
     2281 gagctttatg atcatgaccg catcaatgct gtgatggaca atctgcactt acgcttgatc
     2341 tatggcaatt atccagaggt ttacttaaat ccttcagacg ctgcggagat tctggcagag
     2401 gtcaccggtt cctatctttt ccgcgacgta ctcagctatc aggaagtgag gcgtccagat
     2461 ctgatgcgaa cgctgctcaa agctattgcg ctgcaattgg gaaatcaggt atcttacgcg
     2521 gaacttggca gcatagtggg gctggatgcc aaaaccgtgg agcgatatat ctatttgatg
     2581 gagcagagtt tcatcatctt cagactcggt tcgttcagcc gcaatatcag gactgagctg
     2641 aagcgctcca acaagatctt tttctgggac aacggtattc gcaacgcgtt gatcaacacc
     2701 ttcgatcccc tcaataacag gcaggatgtg ggtgctctat gggagaatta tttcatctca
     2761 gagagacgta agtacatgct caatagacgc gtgggctttg atcattactt ttggcgcacc
     2821 actcagaaac aggagatcga tctgatcgag gtggcaggcc aggacttgcg agcctttgag
     2881 ctaaaatgga gccccaaaaa gaagccctct cctcctctga ctttttcaca gaactatccc
     2941 aatgcccaaa tgttggtgat caatcgggaa aactatcttc cacatctgct taatgagggc
     3001 agtttctcaa aaatgggttg acacccagcc ccctttcaaa aaactggagc caaatcactt
     3061 gtaaatcatc caaggagcaa gataaatgaa acagggactt caccccaagt atgaagttgt
     3121 gaccgtgcgc tgtgcctgcg gcaacacctt cgaaaccaag tccaccaagg gcaacctgca
     3181 ggtggaaatc tgcaacgtct gccatccctt ttacaccggc aaacagaaga tattggacac
     3241 cgccggccgc gtggaaaaat tcaacaagaa atacaatctc aaagccgaca agtagaaacc
     3301 tttagataga aaacgccatt ttgcccggcg gatgctggta atggcgtttt ttgtattcaa
     3361 aggtgttaat ctaacagagg caaacgggga cgtttgccgc tccacaatac tcaaggaaac
     3421 acaatgtcaa aaaaagagat cagcgtaggc gggcaagccg tgatcgaagg cgtgatgatg
     3481 cgcggtccgg aagctctggc cacggctatc cgcagaaaag acggctccat cgagctctac
     3541 aaacagcctt tcaagagcgt cactaccagg ataaaatggc tgggcctgcc catcctgcgt
     3601 ggatttgtca acctcataga aatgatgaag ataggcnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnntttg ccggctccat ccgcatcgtg tttttcgtga tctatgtgtg
     3781 gctgatcagc ctgatgaaag acgtcaagcg tctctttcgc tatcacggcg cggaacacaa
     3841 aaacgtgaat gcctatgaac gcaatatgga cctcagcata gccaacattc aatccaacac
     3901 caccatccat ccccgctgcg gcaccagctt tatgtttttt gtgctgctgg tatctatcct
     3961 ggtcttttcg atcacggaca ccctggtatc catctatatc atcaaaggtc ccgtgccagt
     4021 attgatccgt ctgggctatc actttttgat ggtcccgatc gtatccggcc tcagctatga
     4081 agtactcaag tttagcggca agaatctttc ccacccactc gtcaaactga tgaccgtccc
     4141 agggatggcc ctgcagagga tcaccaccca gcccccggat gacgatatga tcgagaccgc
     4201 cctggtagct atgaaagccg ctctggatat ggattacagc gatcacaagg tcactgtgct
     4261 ggactcgctc aaatgatccc ccgggaaaaa ctcaacgaca ttcacaagga acagcaggag
     4321 ttgcagacaa aggtctcgga taccgctctg atgtcagacg ccagagctta tcgggatctg
     4381 atgcgccgtt acaacgagct gagttccatc accgcctgct ggaacaagtt tcaggagttg
     4441 gaacgccacc ttgaagaagc ccgccatctg ctggccacag agagcgatgc ggaaatgagc
     4501 gctctggcca gagaggaagc ggaacatctg gaacgggaac tggatagctg tgaaagcaag
     4561 ctgcgggagc tgctgatccc ccgtgatcca aatatcgata aaaacgccat catcgaaatc
     4621 cgtgccggga ccggtggcga ggaagccgct ctctttgtgg cagacctcta ccggatgtac
     4681 agttactacg cggatatgaa aggctggaag catcagctca tcgacctcaa cgaaaccggt
     4741 ctggggggtt acaaagagat catctttcag ctctctggcg aggaagctta tggcctgatg
     4801 cgttttgaga gcggagttca ccgcgttcag cgcatcccgg tcaccgaatc cggtggccgc
     4861 atccacactt ccgctgtgag cgtggccgtg ctgccagaag ccgaggaaat cgatctggaa
     4921 atcaatgagg ccgatctaag gatagacgtc tatcgcagca gcggtcatgg cggccaaagt
     4981 gtaaacacta cagactccgc ggtgaggatc acccatgtgc ccacaggaat cgtggtcact
     5041 tgccaggacg aaaaatccca gatcaagaac aaagccaagg ccatgaaggt attgcgctcc
     5101 cgtctcttgg acgctgagat cagccgtcag gaacaggaaa tagccagttc tcgcagggcc
     5161 caagtgagca caggagaccg cagtgccaag atccgcacct acaattttcc ccaatcccgg
     5221 gtcaccgatc acagaattaa cttgacaagc tacaacctcg acggctttct ggcaggcaat
     5281 atcgaagact ttgtccaggc cctcagaatt gcctggcaga gtgagaaggt gaacgaataa
     5341 agatgtcccc cgccgagctt attgtcatag gcacagcgct gttgatgctg ctggccttca
     5401 gcttcgtgtt ttcaggtttc gagacgggca tgatttccat caaccagatc aatctggagc
     5461 ataaagccag acgcaaccgt tcagccgccc ggcttctggc tttcatccga cagccggacc
     5521 gctttctggg caccaccctg atcggcaaca acatcgtcaa cgtgctgctg gcctcgctca
     5581 gcacttatct ggtccaccgc atcgatactc cctggctgcc agccaaatac agcgctatcc
     5641 tgatcgccgt tatcgtactt agttttggcg agatcatccc caaagccatc ttccgggacc
     5701 acgctgaaac catcgtaccg cgcttttttc ctatcctgct gttcttctat tacttactca
     5761 aaccgctggt ctacctggtc agcctgctaa actctgggct caggcgcttc ctgaaactgg
     5821 atgccagggg agattttaac tatctcaccc gtgacgacct cactttcctg ctctcacaga
     5881 cgcaatccga cccctccgag cagccccaga tggaaatgat tgaagacgcc ctggaatttc
     5941 gcgaacaggt ggcccgcaac gtgatggtac ctcgcatgga cattgtcgcg atccctggtt
     6001 ccgccaccat cgaagaggcc atcgccatcg ccagggagga aggttttacc cgctatcccg
     6061 tctatggcca gaacctcgac gacataatcg gcgtcctaat catctacgac atcctcaaac
     6121 gcgactgcac caaggagatg actgctgagc agatcgtcca cgaacccttg ttcgcgcctg
     6181 aaaacacgga cctcgacgtc ctcctcaagg agatgcagca aaagcaccgc accatggcta
     6241 tcatcgtcga tgcctatggc ggcacggctg gaatcgtgac catggaagac attctggaag
     6301 agatcgtggg cgacatcgaa gacgagtatg acgctgagga agaagaggat aaggacgtag
     6361 aacaaatctc tgccaacacc tggctggccc aggccgatgt ggagatcgat cacctggcgg
     6421 acgagcatgg cattgagctt ccagagggtg attacgagac gatcgcgggc ctcattttag
     6481 acaaactgga gcgcattccg catcagggcc agtttatttc agtgcctccc taccgtctgc
     6541 aggttctgca ggccaccgac aaaaagatta tcaaggtcaa aatacataaa ataaacaact
     6601 gaggtgactt atgaagttga tggagtgtgt cccaaacttt agcgaaggac gcgaccaggc
     6661 agttctggac gccatcgcga ccgagatccg tgcggtgaac aacgtggtgc tgcttgacgt
     6721 ggaccccgga gccgatacca accgtaccgt cttcaccatg gccggagacc ctgaagccgt
     6781 ggtggaagcg gcttttctgg ctatccgcaa agccgcggaa ctcatcgata tgagcaaaca
     6841 tcacggagct catcctcgca tgggcgccac ggacgtctgt cccttcattc cgatctccga
     6901 tatgagcatg gatgagtgcg cggagtatgc ccgcaagctt ggaaaacgcg ttggcgaaga
     6961 actcggcatc cccgtttatc tttatgagaa tgccgcttcc aaagaggaat ggcgcaattt
     7021 ggccgtcgtg cgcagcggtg aatacgaagc cctgccccag aaggccaaag accctgcctg
     7081 gaaaccggac ttcggcccgc acgtcttcaa cgccaaaagc ggcgccacgg caatctcagc
     7141 ccgcgaattc ctgatcgcct acaacatcaa cctcaacacc cgcgataaga agaaagccca
     7201 cgacatcgct ctctccatcc gcgaaagcgg taaaccctct cgcgacgcgg ctggaaagct
     7261 gatcaaagac gccagcggca acaaagtgat ggagcccggc ctcttcactc actgcaaagc
     7321 cgtgggctgg tttattgaca gctacgaccg cgctcaaatc tccatcaacc ttaccaatta
     7381 taaggtcacc cctcctcagg cggtgctgga caaagtgcgt gagctggccc ttcagatggg
     7441 cgttcaggtc accggcagcg aactggtggg cctgatcccc aaagccgctc tggtggaagc
     7501 tggaaagcac tatctgcgca agatgaacga gagcaccggc ctgccggaaa agatgatcat
     7561 ggaaaccgcc atccagtcca tgggtctggc tgaactggga ccctttgaac tggataagaa
     7621 ggtgatagaa tatgccatcg cccgcaaaga tggcctggta aaactgcgcc tggatgaatt
     7681 tgccgacctc ctgtccaccg atgctcccgc tccaggtgga ggcagcgtgg cggctctctg
     7741 cgcggctatg tctggagcgc taagtgccat ggtatcaaac cttaccgtgg ataaaaaggg
     7801 ttatgaaagc gtgcaggaca aagtgcgtga actggccccc atcggtcagg agatcaagct
     7861 ccgcgcgctg gaatgcatcg ataaagacac cgacgccttt tataccatga tggacgccat
     7921 gcgcctgccc aaaaagactc ccgaagagat cagtattcgc gacgccgcgg tggaaaagag
     7981 cacccaaaac gccattctga tcccctttga gacgctcaaa atctccctgg aaggtctcga
     8041 actggccaaa caggtggcca tggtgggcaa cgccaacgcc ctcagcgatg ccggagtggc
     8101 cgctctcaca gctctcgcgg ctgccaaagc tgcctattac aacatccgca tcaacttagc
     8161 tggcatcaca gatgacacct tcaaacagga catcctggcc cgctctcagg acctgatcgt
     8221 cctctgcgag aagatcgcca cggaagtgga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn atggcaaagt atgagaaata cctcccggac tactactcga tgagagcagg
     8401 ctttaaactg cgctgaatca gcatactact atgatagaca atcagttgtg cagattactg
     8461 gctgttcctg atctcaaagt gtgagtactc tattcatcaa ccagactaca cccaactgcc
     8521 tgacggtgat ctaccccggg gggaggtcga tcggacggcc ctcaccagca cagccttgta
     8581 tctcgacaac cttatcaccg aaaatctctc caaactgcag atcatcgctt ccacccggga
     8641 agccatagag ggagactggt ctgagctcaa aaaacatctg atgatgatcc ccaatcgtct
     8701 gccgggagtg tggttttatg tgaggccgga cggcaattac tacagtttgg ataaggacct
     8761 cactggcctt aacctccggg accgcggtta ttttgctagc ttgtttgaag gcaagccgat
     8821 cctggggtat ccggttttca gccgttccag cggtaaaaag tcggctgtga tcgccgcccc
     8881 tgtttggact gatggcaagg tcaacggagc cctgggcgct tccatttatc tggatgatct
     8941 gcatcaccgc gtcaacgctg atctggattt gggtattgat tacacctggt ttgccgtcaa
     9001 ctcagagggc ctcacgatgc tggatatgga cgccgatttt atctttatga atgccatcac
     9061 agagagtccg gcctctctga aaagtgccat cgagcaagcg ctccagcagg aatccggaga
     9121 aataagttac gatctcgggg agtttaaacg gcagggacgc tttcaaaagc tgccaaacct
     9181 ggattggtgg ctgatcatgg ccaaaaaggg cctggatgag caaagccctg accctcaaaa
     9241 agaattcaac ctgcagagcc tgatctctga gttgcaaaac agcctggacc ggtgtaatga
     9301 tatagcagta aagcacatgg cagtcaacgg ggatacctgg acgagtgagg cagccatccg
     9361 aagtgtcctc agttccatca tcgcggacag ccctctgatc gtagatgtgg cctgggtgga
     9421 cgctttggga acgatgcgct atatcgagcc tcccgaattt cgcaacgcgg agggagtcga
     9481 gatcatcaag cagccccatg tgcgcgaaac tctggtggag ggcagggagg ttttcagcca
     9541 gtccttcatc tccgcggaag gttttccggc ggtggtgata gcgctacccg tcaagaacgc
     9601 caaagctgag atcctgggag cggtttccat gctgattcgt ccagagctgg tggtcaggag
     9661 agcgctcagc gggatgaaac tgccagcaga tatggaggta tgcgtgatag acaacgcggg
     9721 cagaatcata tttgatgacg acgtcaaaga gattggcctg ctgctgtttg aagaccctct
     9781 gtatcaagaa tatgaaagcc tgctgaagct gggacgccag atcgttgagc agcccagcgg
     9841 caaaggggaa tatgtgtttg aggcgcgcgg aactgaggaa aagaccgtca aagcggcaat
     9901 ctgggatagc gtaggcctct acaacaacca gtggaggata atcatctcct gccagcagga
     9961 ttaagctggc gatgctgaac tggccgtgag tacctccccc acggtgtaga gggaacctcc
    10021 gcagatcaac acgtcatccg gtcccagctc cgccactgcc tgagcgtaag cttctgccac
    10081 actgggatag gcagtggctg gaacggcgtg ttttccaatt tcggcaagct ggtcttcagc
    10141 gctggcggcc cggtctgatt ggttttgggc cacatagata cgagcggcgc gggagcagat
    10201 cagctcgatc atcccgcggt agtctttatc cgccaggatg gagatcacaa agtttatctt
    10261 tttgccgggg aaggctttat ccagagtctt gacgagggct tggatgccat gcaggttgtg
    10321 ggccccatcc aggataatgg gcggttgaat gctgaggacc tgcatgcggc cgcgccagtt
    10381 gatattcata agggcatttc ggacagcggg ttccgatact tcaaaaccct tgatggcggc
    10441 gtattggata aaacaggtga gagcagtggc cagattgacg gcctggtgtt ctcccatgag
    10501 gttggcacgc aggtctttcc agccaatgtc ttcataataa tagtcaaagc agatcccggc
    10561 aatattatcc tgcccggcta tggaaaggta gtccaggccc cagcggtaga taggcacacc
    10621 tttgccctcg gccactttca tgatgacgtg gaggggtgaa ggctcgatgc ttcccaacac
    10681 gagtggcact ccccgtttaa tgattccagc tttttcggcg gcgatgagtt ccagagtgcc
    10741 gcccagggtt ttcacatggt ccaggccgat ggtggtgatg gctgtcacat cgggagtaaa
    10801 gaggttggtg gcgtcgaggc ggcctcccag tcccacctct atgattgagc agtccagttg
    10861 ctcttcagca aagacggcaa aagcgatggc ggtgctgatc tcaaaaaaag aggctgacca
    10921 cttttcgaag atatcttcat agcgcagaaa ggtatccagc acccgctgga aactcagttc
    10981 ctggccatcg atgcggaagc gctccgtgta atcgataaga tggggtgaag tgttgagccc
    11041 tgtgctcaga ccatgggcca tagccagggc ttccagggtg gcgcagacgc tgcctttgcc
    11101 attggtgccg gcgatgtgaa agccgctgag cttttgctga ggagagccca tatccttgag
    11161 cagaccgacc atccgatcca gttccagctt gacgttgccg gagtattttt ggtagatgtg
    11221 gtccagaaat tcctgatagt tcaaaggacc tccctgcggc gatgcatcct atatagagat
    11281 caagggtaaa cggcgatgcc gtttaccctt gaagatcaaa ctcttgggag ctcactccgc
    11341 gactggttcg ggagcggact ggaaattatc cgggtagagc aggcgtttga agtaagcact
    11401 gtctgctttg cgcttggcaa aagcttcccg gtcgtttttc cagaagctga tgtatttcat
    11461 cacggcctgc attttgcggg ggccgttgga tttggtctgc tggtcgatat agtccagatc
    11521 gtccaccatg tagttttgca ggacggtggg gctcatgttt tcatagaaag tcttgggcaa
    11581 aaagtgtttg gggtgttttt cgaggtctgg gcagatcacg atcacgccgg tgttttgctt
    11641 cagctcaccg ctttccaagc gctcttccac atatttgcca tagacgatgt aattggtctc
    11701 atctctctgg atttcggggc agtaagtgat ggaggtgaga tagttgtttt tggcgtcctg
    11761 tttctttttg gtgttgcgca aaacatcgag gtccccacca aagtcggtgc tgaaatcccg
    11821 cacatagtca gtggcggcca cccagatgga tttcatgttg gtggtgcagg tgtcgacgtt
    11881 tttttcggta tcgaggttgt agaaattggg cagagccaga atgaagatca ggcctataaa
    11941 gaggatcgcg ctcagtatcc aatataccga gattcctttc tgattacgca acataagtta
    12001 ctcctgaaac gtttctgcta attacgtaac gcgtgacgcg tgacgcgtga cgcgagatgt
    12061 ccaggggagc caatctctcc aaggcggacg tctcacatca gtggtatctc tcttatttta
    12121 ggggcgtttc ccgtcaagcc ttttttggcg ggcccatgat tttgacgata tagttatagg
    12181 tctccgggat gtcccgaaag cgctgtcgat aatctgctcc gggctctatc ttgatgtgtc
    12241 tgatcctttt agagaccctg gtctcgcccc agttgtaggc tgccagggcc aggctccagt
    12301 ttccatcata acgtttgagc aggtatttaa cgtatttggc agagaggcgg agattgtagt
    12361 agggcacaaa cagcatgccc cgcttgtgtt tctcgtggat atagaaggcg gtggattcac
    12421 ggatctggcc cagtccgatc gcgtccttgg gagaaacggc aaagctgcga aaggcactct
    12481 cggcgcgaat cagcttgtag aaaagcaccg ggtccagtcc atgccagatc gcggtcccca
    12541 ccgtcataag tctcacggag acggggttat agaccaatac cagaaagacc aaaagcagca
    12601 ccgccagccc gatgaagagc cgtttggtcc tgcctttggt cttggggata gttttagcca
    12661 tgagggctat gctgcagccg cgaaggtctc caggagcatt taagcgatgg ttttcttata
    12721 gtcgttgagc atcaggacgt gggagacttc ctcgttgatg atcttgcgca ggatgatttt
    12781 ggcgcgttcg ttggtgatgc tgtcgctgat ggcgtggaaa tagagcaagg tatctttttc
    12841 aaaggtgatg gcgttatcca gcacgcccat gatgtccttg gcctcggtgg cgcgcttgat
    12901 ggcggaagtt tcatcgttga aaatgcggcc atcgacgatg gctttgagat agttggccac
    12961 cagttcccaa tctccggtga gttccaggat gctcatatcc aggtcgtcgc gcaggttcag
    13021 gaaagtcttt tcgtggctga gttcggcgtc acgcagggtc gtgatcagtt tcagagtcct
    13081 ggcatccagg tctttgcgtt tgctggcttc gtgatagtag gcatagccgt tgcgctcgat
    13141 ctgcacggcc atttcgatga tctcattgac ggaatatact gccattgttc ctccagggga
    13201 ttatttagtt attcactgct gatagtatct cggaaagggg tgtttttgtc aagtacaaaa
    13261 gcggctgcca tgatatttct gcttgtcaat gaatccgggg caggttatcc tttcacaaag
    13321 aaatacttat ctgatcaagg agagacatcc atgcgcaaag ccttgccaca atcgcataca
    13381 ttcgcagtct tgagaccgat aaatggatcg acagcccttt ccgcttagcc acccatgctt
    13441 atcccatgct tatcgcacgc ctatcccacg cttcagcatg ggataggcat gggtgacgca
    13501 taagcggtga gggtctggat gttttgggcg gtgcagaggg gggatatgga cttttgagag
    13561 ggttgcaggc atcttctcaa ggcctgtccc cgatgaagat agcatggtgg tcagccggca
    13621 gattgtaagt atcaccccat taaacatata gctccctctc ggcaggcatg tcattcctgg
    13681 ctggggtcgc ttgcggtccc gccagtctgg tcagactgca taatgccggc aaaaaaagag
    13741 cttgactgtc agacaaacgc tgaaaacaat gccgaacctg agtctgaagc ccagcttcgg
    13801 gtgtctttca ctgggaatag atctcaggat gacaatggta gtccggaagc gctctccggt
    13861 gatgccaatc cagcaatgag cgagataata gcgcaatctc agccaaaggg gaacaagatg
    13921 aattcagtgg agcagaacga aagattggtt ttagacccaa acacgatcag ggaactgtgg
    13981 tcccggactt ataaccgcga gggaaagccg gactggtctc acatctttcc ctattaccat
    14041 cggaatatcg tgtttcagga caccatccag cgcatcgaag gcattggtaa cttcatcgcc
    14101 atgtgcaaac ggctgacccg tcggacccgc cagctcaaga tggagattct ggccatctcc
    14161 caaactgaca atgtgatcct gtttgactgg gtcatgacca tgatgtttaa aaaatacccc
    14221 aatactccca tttatggggc caccaaactg gtgttgagcg aggacggtct catcaaagag
    14281 cagcgggatt attacgatct gtggggcgat atctttaaca acatccctca cttcaacaag
    14341 atgtaccgca gatttctggc caagaaattc ggatgagcca gcgccgtgaa taataaaacc
    14401 agacaataca tccgccagta taagtggtcc aacatcttcg cgatggtccg caacaacctc
    14461 agggactcca aactctgccg tgacgactgc cagggcaggt tggtagtgat cacaggcgct
    14521 acttcgggga ttggctatgc cgcggcccgg aaatatgctt cccgtggggc taaactctta
    14581 tgcgtcaatc ggaatctggc gaagtcccag cgcctgcaaa aggaaatcca gaacgaattt
    14641 gggacagagt gtgagtttct gatcgccgac ctgagcaagc tgagcgatat ccaccgggta
    14701 gcggatgagc tggcacgcct ggaaacccct atcaatgtac ttatccacaa cgcgggcgtc
    14761 tatctcacca ggcgggaaat cacctccgac ggcctcgaga aggtgtgggt ggtgcattat
    14821 ctggcctcgt tcatcatcaa ttaccgactg atggacaagc tcagagctca agaccgggcc
    14881 cggatcatca tggtgggctc ggaagggcat cgctttgccg cctggggctt gaggctggac
    14941 gatctgaact gggaaaagcg cagatacagt gggctgggca gctatggttc cgcgaaactg
    15001 gcacaactgc tggccatgat atattttaac gagcaatttc ggaacagcgg agtgagcatc
    15061 aacaccatgc accccggggc cgtaaagacc gaaacgggcc aggaaaacgg agcgctctac
    15121 cgctggttta aaaagcactg ctttgaccgg accctgaaat ctcccctcgt gtctgccgaa
    15181 gctctctact atctgggggt ttcaaaagaa gtggatggtg tgagcggtaa gttctttaat
    15241 ctcagcacta tggaggatcc agcccctcct gccatggata gagacgtggc cgggcagctt
    15301 tgggaaatta ccctggccat gagtggcctg ctgaacaacc cggagcaaga aatctaacaa
    15361 catatgaata aacatagtta cgatactata gtcgtgggcg gtggtatggc tggattgacc
    15421 gccaccgctt atctggcccg ggccgggcaa aaggtgctgc tgatcgaaaa gaacgccgaa
    15481 ctgggtggct tggtaagttc cttttcaagc gatggtttcc tgtttgacgc gggagtgagg
    15541 gccttggaat ccgcggggat tattctcccc atgctggctg aactgcaaat caagctggaa
    15601 gtggtgaaaa gccctgtttc attgggcatt gaagatcaaa tcctggctat cgacaacatc
    15661 gcgagcctga ccagatacag agacctgctc aaacacctat atcctgccag tgccgctgag
    15721 atcgatgcgt tgatcgatac tatacgcaag atcatgaaag atatggacgt cctctatggc
    15781 gtagagaatc cgcttttcaa ggacctggtc catgaccggg agttcatctt caagaagctg
    15841 ttgccctggc tgccaagatt cctgctcacc atcggcaaaa tcaaccgtat gacctatcca
    15901 gttgaggact tcctgcgctc cgcactcagc gatgattccc tccgaaacat catctctcag
    15961 cactttttca agggcacccc cacctttttt gctatgagct atttctctct gtacctggat
    16021 tatttctatc caaaaggcgg agtgagcaaa ctcgcagagg ccctccggga caagattctg
    16081 gcgctgggtg gagagatcag aactgcgacc actgtaacgc gtgtggcggt gggcaaccaa
    16141 accctaagcg atcatctaaa agaagactac agctatagaa agctgatctg ggctgcagac
    16201 ctgaaaactc tctaccgcat agcggatacc cgaggactga cagccaggct ccaaaccagg
    16261 attgggaccg tcaagaccag aatgctgcaa aaccggggca cggattcagt cttcactatc
    16321 ttccttcagg tggatgaagc tcctcaaagc tttgccgcgg ttgccaatgg ccacttcttc
    16381 tatacttcct cacgtcaggg gctgcgtgaa actcaccgca aggaactgca ggacctgctg
    16441 catagttttg gagcgaagag caagccggag ctgctggcct ggctggataa gttcatctcc
    16501 ctgaacacct tcgagatatc gattccggtg ctcaaagatc ctgatgcggc cccacccggt
    16561 aaaaccggag tcattgtgag ttttctggca gagtatgagc tcttccgggc tgtggaagta
    16621 gctggctggc tggatgagtt tgtcaccctg atcgaggacc gggtgatcaa agtgctggcg
    16681 gaatcggtgt atcccatgct gaaagacaag gtgctgaagc gctggtcata ttcgccgctc
    16741 agcattcaaa agcgggtggg cagttctgag ggagcgatcg tgggatggga gtttcaggct
    16801 cagatgccgg tggtcaatca gatccagaat gccgcccggg ccgtgttgac acccctgcct
    16861 tcagtgtatc aggcaggaca gtgggcttac agtcctgccg gagtgccgat ggcaatcctg
    16921 acggggaaga ttgcggccaa ccgggtgatc aaaacagcca gaaagggctg atccgctcag
    16981 ggcttggttg atcttttcag atcagatcag gctgtccagc tggtccacga ttttgcgttt
    17041 aatagtttcg cggcacttcc ggaaggcctc gtcactgcct tcctcggaaa gccagacctg
    17101 ggcggtgggg tcagcgatgc ctatatgtat ctgttttaca ggccttaaga atacgggaca
    17161 gacctcgcgg gcatgatcac agacagtgat caccagatcg agatcgtctc tgtcaaggaa
    17221 ttcctccacg cttttggagc gcaggctgga agggtctatt cccaattccc gcaaggcctt
    17281 gatgctgcga ggatggactc tggagggtga aactcccgcg gaccaggcct gccaggtatc
    17341 gcccttcaag tggttgatca aagcttccgc gatgatgctg cggcaggaat ttcccgtgca
    17401 gaggaccagt accttatgct tggctttcat cagctaattg gcctggggac ggggtcccat
    17461 gctcagagcc cctgtgaagc actttttcac acacagcgag cagcccacgc acttgtccct
    17521 gtcgatcacg ggatatctgt catcgtcaag cgtgatagcc agataggggc aacgggtgca
    17581 gtttccgcag tttacgcaga ggtctttgtc cagggttgaa atggtgtggg agggggccag
    17641 gtccataaag tctgtgatgg gatcgggttg cgcgatgccg atcagctcct tgacgctctt
    17701 tatgccttta agttgcatat aatgactgag gccgctgcgc agctcgtcga tgatctcaac
    17761 gccatacttt tcggcaatgc tgcagaactg caccgtctcg gctcccaaag ccaggaaatt
    17821 ggcagcgccc agatagtcca tggggcctcc gtttccactg atggacactc ccagtttggc
    17881 ggccctggcg atggtgagat agctgatggg cagcacgcct tctccgctca tgcccacgat
    17941 catagcttca tcccagagag ccgtgtctgg cctgtcagat gggtacttat cccggaaggc
    18001 cagagtggga aaggtgtttg ccagagtgat gccggccttc ttgccgggat agcggtccag
    18061 gacgtcccga atggccttga cgattggata gatggcagtt acggctccag tgagcttgaa
    18121 gagcttgggg atatcgggat gggaaatctc catcacccaa tccacgattt tagcagtaag
    18181 agcggcatcc tgagacacga tgtcgccatg ggtgccatct cctccctggg gacaggaaag
    18241 actgtattca atacccatca cgccactggc ttccagcttg cgggtgttgg cctgccagcc
    18301 ttgtttatct tcctcgtcgt ttccggacac gggacctccg gtgctggcca tggtgagtct
    18361 atcaggaaat tctgtgacga gctgtttgat ctcagcgcag acgcggtcca gaggatggtc
    18421 ggagacattg tcgcaattgc cgtaggtgtt gtcgttgaag accaccatat actcgccggg
    18481 gatgtggata ctcacgttgt cgaaggcggt tttcatgatg ccaccgctcc agccagcctt
    18541 atatgctttc agcatctgat catagccatc ggtaggaggg gcggccgaca gcagaaatgg
    18601 agctgagatt tcacggccaa agaaatcagt cttgagcggg actggctctt tgacccaacc
    18661 aaagatggga ccgatgcttt tggtggcttt gggaaagctg ggctgggctt tggcatttag
    18721 ggcacagtcg actctcaatg ccacgttttt cccggccgcg acggcttcca ccactgtact
    18781 gggaccgttg gacatatcgc ctgcctgcca gatgccgtct ccctcttcac ggatggaagg
    18841 acggtttccg atggcaatga aaagcgcgtc gaagggcctg aatagctctc cgctgcccag
    18901 ttcgtccact attttggcgg ggtggaattt ctcgcccttg ggaagggtga cgggacggat
    18961 ctgtatgcca gtcataacgc cattctcagc aatcacttcc gtgatccggc tacggtttgt
    19021 gaactgcact ccggcctggg tgagaccctc tctttccaca ggggtgagag gcatttccag
    19081 ccaggactcc agggtgatca tctctacatg ggcggcacct ttctggactg ccgtgagggc
    19141 catatccgct gcaatggcac cacctacgag ggctacgcgt ttgcctttaa ggtcatagcg
    19201 gtcgggattg cgaagcagat caaagccaaa gaaagccgct tcggcacctg ggatgttcag
    19261 acggatgggc tgatcgaggc ccacactctg gatgatggcg tcgtagcgtc ctttgagctc
    19321 atcgaggctg gcgaatttgc ggtcatagct gatcttgacc tggaaagtat ttaccatgaa
    19381 ctccaaatcg ctgctcagga tgtctttatc cagtctcgcg tccgggatca gattagcctg
    19441 acctccggca atatcttgct cttcaaagac ttccacctca tagccaagct ggatgagggt
    19501 tacagccgct ccaatgcctg agggtccggc cccgatgatg gctaccttgc gtccgttggg
    19561 ctgggggtgg atgaattccg gcatctggcc aagctctttg gctttttgca cgatcgtagc
    19621 ctggacggcc ggaatttgga tggcgcagtc aaacttctct ctggagcagg cagcctggca
    19681 gtgataatca ggacacacgg ccccgcagat ccctcccaaa ggattgctgg ccaggatgat
    19741 acccgcggcg cgtttaaaat ctgatggctt accgcctctg gcagccatga taaaatcggc
    19801 aggggagcaa tcagccgggc aggctgtctt gcaaggtttt tccgcgcagt attcacagcg
    19861 ctgcagttca ctcttgagtt gggcatcggt caaaaacatc tctatctcct tggagggatt
    19921 atattaacag tgagcgtcaa ggatcatgga caggcaggtt tttgtcaatt ggaaagaggt
    19981 accggtttag taactttatg ccttgcatgg aaatggccat ctctacgtgc tctggcccaa
    20041 gcgggtttgc ggagatcacc ggaagaaatt acgttgacac aatctcccag ttcagatact
    20101 ttgcccgtta tcataagact aacggagcat cgatgcaaac agacgttgta aaatacttta
    20161 tggagctggt cgcgatcgac agcgaatcca aaaacgaacg cgccatggcg gacaggatca
    20221 tggcagactt gatctccctt ggcgctcagg tatatgaaga tgacgcccac aagcagactg
    20281 gcggcaatgc cggaagcgtt tacgcctggt ttgatggtga tccttccaag accgccatcc
    20341 ttttctgcgc gcatctggat accgtgaaac ccggcaatgg aatcaaagct ttgatcacgg
    20401 acgggcgtat ccacaccgac ggaaccacaa ttctgggtgg agacgataaa tccggagttg
    20461 tggaaatcat ggccggcatc cagagagtgc tggcaagcgg tgcctcgcat gcccccatcg
    20521 aagtgctgtt caccatttcg gaagagatcg gccttttggg cgcaaagctc tttgacaaga
    20581 gcaaactaaa agccaggatg ggttacgctc tggatactca cagagtggga gaactggtgt
    20641 tggccgcccc atcccaaaac tctatccgga tctgcgtcaa cggagtggaa gcccatgccg
    20701 gagtcgagcc agaaaaaggc atcaacgcga tccgcgtagc ctctgaagcc atcgcctcca
    20761 tgcccatggg aaggatcgac cacgagacca catgcaacat aggagtgatc agcggtggca
    20821 ttgccacaaa catagttcca aatcaggtga tcatccgtgg agaagcccgc agccacaata
    20881 tggccaagct ggagcaagtg agttccgata tccgtcatgc cgtggaagct gccgtcaaac
    20941 gtcatcattt ctcccagggt tccgctacct acgactggca gtgtaaacgc gagtatgacg
    21001 ctttccacat cggggaagat gaaccggtgg taaaactggc caaggctgct ctcgcggagc
    21061 tcggtatcga gtgcgacgct atcagcggag gtggtggcag cgatgccaat atcatcaacg
    21121 cagccggcat tcctaccatt atctgcggca cgggcatgaa caaagtgcac accgtgcacg
    21181 aagacttgct ggtttcggag ctggagagag gcacctgctt tatcgaatcg ctgatcaaca
    21241 tccattcacg ctaggaggca ctatgatcaa gattggactt atcggctatg gcaagatggg
    21301 caaaatgatc gcccaactgg ctgaaagcca cggctgccag gtggtggcca tcatcgatcc
    21361 caaacatccg gaccagcatg cccagatcaa cccaaacgtc ctggctgggg ctgacgtctg
    21421 cctggaattc acccatccgg actccgtgat ggaaaacatc cgtcagctgg ccgccctcag
    21481 gaaacacatc gtgatcggga ccactggatg gaacgagcat ctggacgaag cccgcaacct
    21541 ggccatccgc tatcagatcg gcattatcca cggagccaat ttctcaatcg ggatgaatct
    21601 cttcaaccgt ctggtagcct acgcgtctca attggcggac aaatttccgg attacgacgt
    21661 ctggggcatg gaaatgcatc acaaccaaaa ggccgacagc ccttccggga ctgctatcca
    21721 actggccgag acggtgattg ccaaccactc ccgcaagacc actgccatgt atgaaaccct
    21781 gcaccgcaga gcaaaacctg aagagctgca tttctccagc gtgagaggtg gttacgtgcc
    21841 agggctgcat cgcatcggtt ttgattccga agcggattcc atcatgctgg aacacagtgt
    21901 ccgggatcgt tcctgtctgg cagcgggagc tttgcaagcg gcaatctgga tcagtgaacg
    21961 ccctggtttc tttagcttta acgatatgat ggactcccgt ctgtgctgat caacttcatc
    22021 aaaatgcagg cccagggcaa tgactttgta atgctcaact tgctgggtaa caaagaactg
    22081 ccgctggatc cccccggact ggccaaggca atttgcgatc gcagactggg ggtgggagcg
    22141 gatggattgg tactcctgag ggatgacgag attgccgccg cccgcatgat tatctacaat
    22201 tcggacggca gccgggccgc catgtgtgga tcggctttgc gctgttccgc ctggcttctg
    22261 gccgggcagg gcaaaaaaaa ccagctgttg atttccaccg attccgggcc caagagtgtg
    22321 gaggtggagt acacggacaa gatggtgcac gccaatctgg gagctcccag gctgatggaa
    22381 gcgaagcttg aactggaggg gctcgaaggc agcctggtgg atgtgggaaa cctgcatttc
    22441 atcagctggt gggatgctct ggatgatgat cctcatctcc aatatggcta tagcttagag
    22501 cattcaccag cctacgaaca gggcctcaat gccatgtttg ccagggtcgt ctctcctcag
    22561 gaaatcgatc tgaaaatatg ggaaaacgcc tgtgggccta cgttggcctg tggaacgggc
    22621 gctaccgcaa ccgtgagagc tggcctccaa aaaggcctgt tgagtggaga ggtgaaggtc
    22681 aacatgccag gggggacggt ctctgtaaaa gaggactccg cgggtgattt tatcctgagc
    22741 ggttatgtgg aagatgtgtt taacggagct taccgatgga aaatttaggc ctctatctga
    22801 agcaattcag agaagaccag ggaatagaat acatcacggt ttacagcgat atccgtctgc
    22861 gggaagagca agtgaagctg atggaggaaa accgcttttt tgagctgggg ccctacggcg
    22921 tggtcaaagc catggtcttt aactatgccc gctacctggg ggccgatttg gatgccgtga
    22981 tgacggaatt gaaggtcctg gtgccggaag tcaccaaaag tcatcaccgg cctgcccgtc
    23041 cccgcaaaca aaaaaagatc atgctgtcca ccaacttcct ctggggcatc gggatactgg
    23101 tctttgtggc catcctgggc ggcatcctct tccatgctta caaccagggc tggctcaaaa
    23161 ctccccagat tctcaaatcc gccgcaaccg acagcctgat cgtggtgcca gccgaacctc
    23221 ctgaacccga acagctcgcg ccagacagca cccggctcag aatgaggcag ctgacccagg
    23281 agctcaacgc tcctcccaaa gaagaaccgg tcaagcccaa agccgccgta aaagctgtca
    23341 aaccagacag cacagactat atcaacagct ttatgggcga cagtcccgtg aatgttgaga
    23401 cacgctaagg tccagcgcta atttcctttt cctgatggca gcggcgggat ggatccctcg
    23461 gcggagcagg atgcctgtca tgactgccaa acagctttgg tcagagtact gacaaacacg
    23521 catcaaagcg ttcaggacaa gagcgttccg gtagtctata agcgcttgcg cagctctggc
    23581 ttcagccttt acaaccgtct tttcttctgc tcatctggag cttgagcgca aaaaacatcc
    23641 accgggcaat cctgatacac gagttaaatg ctattgatac agagcattgc atcatcaata
    23701 tccatattgg acccccaaaa atgccatctt ctctttgcct cctctccgat tagcattacg
    23761 gagtcattac ggactcatta cggactaagt ccgtaatgag tccgtaatag gatgcaggga
    23821 ggatagggag agaagctgat ccatgaaggc atgtatgcca gaaaatacag aatatttcgc
    23881 tatagtgaca aacagttcaa accatcccgg cctttggaat gtttattgct ccctcaaaac
    23941 aaatcatgca aggcaaacga gcttctggct gatagccgga acttccttta aggcatcaaa
    24001 atacactttc ctgggaggag agatgaagcg aagcctgttc gtcatcgcgg cagttatttt
    24061 gctgatcggc actttgacag cccaacccaa cgagtttgaa ctattgggcc aggggcagtt
    24121 ttctggcaag ctgggcagct atgtgtctgg ggatacaatc ttttgcgtct ataacaccgc
    24181 cactctggct tccggaaccg gcagcgtgat ccttaaagcc tcttatgacg gaggggaaac
    24241 ctggcaaagc cacacggtgg ccgaaaccgt taccggggtc ggaaacccca ccgtttcctg
    24301 gtctccgggg gaacttatcg tgtgctatgt ctccggtttt gagcgctata tagccaggtc
    24361 atccaatgga ggtcagaatt ggcagacagc ctctttcggc cgcagctttg aatccagtcc
    24421 ctacgtggaa aagatcgatg gcgagtatca cactttctct ctggatctgc catatcccca
    24481 atgggatcag gatagatatc tactctcacc cgaatcccaa agcctgagga tgccgcaata
    24541 cctgactgat atggaagagt atcccagttc ggaaacctcc actttcaatg ggtatgacgt
    24601 tttacatggt ggggtccgca ctaacaatga tctctggatc cggcagatgg gtggcggaga
    24661 aaacaacggt tggcccacct tctggggccc ggtgatctgt ggaggaacgg tacgcgttta
    24721 tcccgatggg ggcacaaact atccggccgt ggagattttc cggggtggct tgattgagca
    24781 cgctccccgg ttggatcctc cattgcccaa tcgcagagat gccataataa tcggcccggc
    24841 ctactatgat cccaatcaga tcgtgctgat cgaggtggat ggcagtacct accatggcaa
    24901 gcttggaacg atcacaccgc ccatcgctca aagcgcgaat gtctatgata tctatcccgc
    24961 cagccctctg ggagagccgc tctacactaa tcaatacaca gaagtcgata ctgtctgggc
    25021 cttgctcggt tcaggtactt gcaacaacaa aatgttcttc tcacatgggc ctttgtggat
    25081 caagggcaat ttctccggcc accagtcctg gcaaagtgga gcagatattt atatcatagg
    25141 tgacattacg ctatcgggta cacagcctgg tctggacccg gccgataatc tcaatgattc
    25201 cgtatctctc ctctccgaga agaatgttat tgtcaaatac ggatacagag atcctgagga
    25261 tagcctgcgc tatcatctgg cccggagtga cgatgatccc atttacatct atgccgatat
    25321 ctcggcagcg ggattgtcac ccaacctcgg aatgttcact tatgagtatc aacaacccca
    25381 tccttctgtc ccctcggtca atcacctcgg tcaattctgg gatcaaatcg atctgcatcg
    25441 gcggccctat cctcagacag ctgcacagcc ctggcctgcc aatatcgatt atccctggta
    25501 caatcccctt tggccggaag ccaaaccata tttggaacgg ggtaagctgc aaatctgggg
    25561 ttcggtgatg caacggcgca ggggcttttt gcacagatca ctgagtgata ttaataatcc
    25621 taccggtatc tggaatcctg cccagaatct ctttggaggt tccagttcac ccactttcac
    25681 caatcacctc gatcctgttt tgggcataga aatgggtacc caaaactatc caggagccac
    25741 tgggaacggg attggctata aaaaggacca tcattataat tcccggggaa tcttcggggt
    25801 tacagatcag gatatctgga cagccaatga atccatgtgt tggaatctgg gcatacatgc
    25861 aggacaatta tctgcaggac cagacaatag ctttgtagaa gaccagtttt atccatacca
    25921 aatgatgcag ctcagaaagt ttcgctccaa agcctatgcc cgcagaggca accgtttcct
    25981 gcacgccttc aacgaacgcg tcctctatct aaacggtcaa aaccccgtgg agataggcta
    26041 tccgctcttt gagggtggag acgttatggg catccagtgg ctcaacgacg atcaggccct
    26101 gctgcaccga tacagataca acgcgccaac agatcaacgc tatctcgaga tcctcgatct
    26161 ggatacccag gctcaccaag ctctgaacat cccagggggc gaagctccct accctcagga
    26221 ctccccctta agcgacatcc tcgtaataga agacggcaca gccttctttg gcagatacga
    26281 tccggagttt agcctcctga ggttatggac tttggggcag gataaccagt tcagcccggc
    26341 cgggcaatgg aatctgcctg cgagcgagac tggaacaccc gggccgggaa gcaagctggt
    26401 gctgcaatcg ggaaccggtt ccatgatcgg cgcgctgctg tggtgggaat atccagccga
    26461 cgacacccat ccttatccct ggggcaagtt gtatggcgcg aggatagacg cggccaatac
    26521 tcccaattcc gatcccgtca ttccagccag gattccaacc atgaatgtgt ggcccaatcc
    26581 ctccaggacg atgatcaatc tggagatcaa aggcctggct tccgaggccc tgaaagcgga
    26641 agtctacaat atccgcggcc agaaagttgc cagtctgtca ggctttgaca gaaaggaaga
    26701 tggcagcctc agcagtacct ggaacggcag cgattcaagc tctcagagat tgcccgctgg
    26761 cgtctatctg atcagagtaa aagccggcag ggatgtgaag ctagtgaagc gggtaagtat
    26821 atattaatca gttttaagcc attaaccccc tgtaaggcat cgctcatgtg atatggcgga
    26881 gctgtgattt attctggtcc ggttgtgtat tatgcctttt tagattgaca gaaatcacta
    26941 tcttatccat aatgacggtc aatatcatag acggagcaga gatgtctcag caagcccaca
    27001 gttttcacga attcatgcca gcggacgttt ggcgctggaa aatgatcgag caccaggtgg
    27061 accaggtgct ggccctgcat gactatgaag aaatccggct atcggtgttg caggattaca
    27121 gcgtgctcta tcaagggctc acagccctga tgcatcagca gcaggctgag gaagcggtac
    27181 gcaagaccct caacctctgc gtgcccaacg atgacatcag cctcatatcc ctgcgtccgg
    27241 aaggcactat cagtgtgctg caccataccg ccagggtttt ccagcctggc gagatccatc
    27301 gcttttacta tcacgggccg atgttccgtc tggacaagaa tcagcgtccc atggagtttt
    27361 atcagctggg tgtagagctt ctgggcagcg agaccattct cagtgagagc gaagtaatct
    27421 ctttggggat caagctttgc gaagctttgg gactgggaga cgtgaagctg aggatcaaca
    27481 gctatgggtg caagcagtgc cggcataagt tctttgagga ggtcgaaaat tatctcagct
    27541 cccatcagga tgatttctgc gccaagtgct atcagagcct gcggcacaat ccctttgagg
    27601 acacccactg cgacgacccc cgctgtcatc atctgatcaa ggaagggcct aagatcaccg
    27661 accacctctg tgaacgctgc aaggtggatt ttgcccgcat caaaaagatc caggccaatc
    27721 tgggccatcg ctacacggtg gacccctttc tctacaagaa cttttcctac tacaacgaga
    27781 ccgtgtttga ctttgtgctg ggcaacggca agcaagattt aaccatcgga ggcggaggca
    27841 gatacgacga tctatctgag cttatcaccg gcaagcgcat ccccgccgtg ggtttctatc
    27901 tcaatatgga caccatattt gcagtgctaa agcagcgagg cctgttcaat cctttggccc
    27961 aggcgttttc agtttattta tgcgcccaat ccccagacat ggagatgatg ctcctgcaga
    28021 tcgctcacga gctgcacagc ctgggagtga ggaccgtatt gggcatcgat cacgaccccc
    28081 tggagcgtca gattcgcaag gccaggggca aagagtgcgc tctgctgttt gccatccggg
    28141 aagaaaacat ccgggacggc aagatactgt tgcacaacct cgtcaaggac catcaggagt
    28201 atatcccttt ggcccaggtg gtggatgccg tcctgattgc ccgtaaagcc ttaaaccggt
    28261 aatagattat cctccaagga gatataatgc atagcatcgt tacatccaat gcccccgcgg
    28321 ctttgggacc ctattcccag gccgtattga aaaatgacac cctgtttgtt tccggtcagc
    28381 tgggcttcga cctcaaagac ggcagcatgc ctgaaacctt tgaagcgcaa gccttactgg
    28441 tattctcaca cctcagggct attcttgatg ccgctggcat gagcatggcc aacgtggtga
    28501 aagtgagcgt ctttctgcag gatatgaaca acttcgccaa gctcaacgag atctacgccc
    28561 gcaacttcag tgctcctttt ccagcccgtg aagcgatcca ggtcgcccgc ctccccaaag
    28621 acggactgat cgagatctca ttgatcgcga tgaggtgacc catgagcatc accaccaaga
    28681 ccggagacgg aggccagacc tcgctttaca gcggtcagag ggtttggaag gatgagctca
    28741 gggtagatgc ctacgggacc ctggatgagc tggacgccca cattggggac gcctgtcatc
    28801 tgatcgaaga cgatgagctg gtgcagtcgc tgcggggagt gcaaaacctc ctctacagag
    28861 cgatgggcca gctcgccacc ctgggagagg gcttccctta tcctatttgc gccaccgacg
    28921 cggaacagct cacctccatg atccacggcc ttgaagagca aacccccatc accggttttg
    28981 tgattccagg cagcctgcct gcctctgcca gattggatat ctgccgcacc attgccagaa
    29041 gggccgagcg cagagtgatc gccctcaatc gtcaggaacc cgtaaaccct catctgatgg
    29101 gcctcgtcaa ccggctttcg gacttctttt acatcctggc ccgccatctg gaagaccgcg
    29161 cgggagtcct cacctacaaa agcaaaccag agcagcccgg ctgcttgagt tgagatggct
    29221 gaaagggttt tgacggttga tggcccagat ccacagacgg gacaagcttt tcagccctta
    29281 caaccccctc aaccctttca acaaagcaaa cccaggagct aacaaatgaa acaaatagta
    29341 ctcatgcgtc acggcgaaag cgtgtggaat aaggagaacc tcttcaccgg ctggacagac
    29401 gtggacctct ctgacaaagg cctcaccgaa gcccacgagg ctggaaagcg tctcagagaa
    29461 gccggttttg agtttgacct ctgctacacc agcgtgctca aacgcgccat ccgcaccctc
    29521 tggaccgctc tggacgagat ggaccgcatg tatttgccca tcctcaacga ctggcggctc
    29581 aacgaacgtc actacggcgc cctgcaagga ctcaacaaag ctgaaacggc agcccgttat
    29641 ggagaggacc aggtgaaaat ctggcgacgc agctttgacg tccctcctcc cgctctggac
    29701 accagcgacg aacgctatcc cggctttgac ccacgctaca aaggtctcag ccagcagcag
    29761 ttacccacct gcgaatcgct caaagacacc tgtgccagag ctttgccttt ctggaacgag
    29821 gtgatcgtgc cccagcttat gagcggcacc cgtatgatca tcagcgccca tggcaacagc
    29881 ctcagggcca tcgtcaagca tctcgacgag atctcagacg aagacatcgt gggcctcaac
    29941 atccccaccg gcatcccgct cgtctatgag tttgacgacc atcttaacgt gatgaaccgc
    30001 tattacctgg ccagtgacga agagctggct tcggcccaaa ctgccgtcgc caatcagggc
    30061 aaggccaaat aaggatagaa acgggttggg atttttcgct tgacgaatcc accccatcca
    30121 aaagcatagc aaaaaaacca agggagaaca acatggctgt caagatcgat aaagaaactt
    30181 gcatcggctg cggcgcctgc gtggacgtct gcccggtcag cgctctttcc atggaagatg
    30241 gcaaggccgt ttgcgatgaa aacgcttgca tcgactgcgg agcctgcatc ggcacctgcc
    30301 cggtcagcgc tatcagcgaa taatcgctag cttatgaatt gaggaagaag tccccaccaa
    30361 agggatcttc ttccttttct tttcacctga gacctgagtg tggttcactc tggtgaggct
    30421 gcctggaaca aaacttgctc caggtaacac cataaagcaa aaggagttgt caccatgaaa
    30481 agcacttcta tgatcattgc gttgttgata tttagcgtca gtttgctggc tactgatgtg
    30541 agcggtgtcc agtccggcat ttggaccctt gccaacagcc cctacaacct gattggagac
    30601 gtcagtatcc catccggggc ttccctggtc atcaatgccg gtgtaactgt ctatgccatg
    30661 ggcaactacc gcatcaatgc cgaaggcact ctgttcgcca acggaactcc ggcggacagc
    30721 atcaggttta tgaacggcca ggcggatccc aacgccctct gggtgggtat ccggctggaa
    30781 aatgagaaca ttcccagcag cattacctac tgctacattg agaacgcctc ttatggagtc
    30841 aacagcgtca atagccccat tgagatcgcc tattgccgtt tcaataaaaa ccagaaaggc
    30901 atgcagctct atggaatagg aagtgccaat ccagccggga tggacgtgca tcacaacctc
    30961 atcgagcgca gcatccaaaa cggaatcctg attccgcaaa actccaacgc ccacgtgcat
    31021 cacaacgaac tgcgcttcaa cggcaccggc actcaataca tggccgccat ccagctctcc
    31081 aaccagtcca ccgggggaga aaacaaccct gagatcaacg acaacttcat ccatcacaac
    31141 tacaaacagg gcatcacggc ctgggacatc gttggcgcca acgctatcca gccgcatatc
    31201 catcacaaca tcatagaata taacctcaca ggcatttacc tgctgaacgc ctccggctat
    31261 gtggaggaaa acgtcatccg ctccaacttc atctccggag acaccaattc cggggccgga
    31321 gtgatggtgg cgggagctac cagcgcgcct tatttcgagc gcaaccagat ctacggcaac
    31381 ttcacaggct tttatctggg caccaacgct cagcccgttt tgggggacat gagcatctac
    31441 cacgcctggg cccagggcga aaaccaaatc tacgacaaca tcgacgaaag cagcactctc
    31501 catagcgttt acacctattc ctacaccaat tccgccatcg tcatcaaagc ggaaaacaac
    31561 ttctggggta gcaacgatcc cgcccagatc aacctgggca tcaacgacca gctcgattcc
    31621 ccctctctgc ctttggtgga ttacgatccc tggtattggg agccggaacc caacaccctg
    31681 ctgaccggaa cgatcgtgta tgaaggtagc caggccttgc acaactaccg catcgagatc
    31741 gtgggaaccg tgtcagaaca ggtggagcac agctttgagg tggaatatgc cacccccttc
    31801 agcctggctt tcgagatcga cgagccctac cacgtggtgg ccaaagccga tattgttggt
    31861 cagcttaaga ccctttacgg cagcccggga ggctattggc tgactgacac cttccagcct
    31921 ggtatgacca gcgacacggc cgcgtttttc ctggaggacg atcttcctcc catagcagta
    31981 gtggccaccg aaccaatcca ggaagacggc cacaccctct ttcccgtcta tcacaaactc
    32041 tggatctatc actgggacca catcgactgg ctctatgagg acggtgattt ccgctatctg
    32101 aaacgccaca cccgcttcca tccggatggc aatctggagt ttagcctgcc tcccaacagc
    32161 gtttacgaca agtttcaaaa cctccagccc ggcgacatct ggacccgtac atcaatctat
    32221 aacgaccagg gcagcatccg cacttctcaa ctgcgctatg tggaactgga cgacggtgcc
    32281 gctatagatg gcagccgctt tccagtgcaa ctgatcaccc agatcaacat ggcaaacgtg
    32341 tctctgatga gtcagaacct cattacgggt gaggacagag ggattgtctt tatctacgac
    32401 aacaacggca atgtgacaca gatgatgagc tatctcacca atctgctcga tacccctctt
    32461 gcctggggca gcttcatcaa ttacgatgac ttcgaatatg taacctcgcc tcagttcctc
    32521 tgctacgatc ctgtccggct gcagaatgaa ggtatcatgg aactctattg gcaggccccc
    32581 gcttcagacc atcaacacac ctggtcccag tatcgcgtct atgacaacgg cattttggtg
    32641 gccacggaca gctacgggtt tcccaccacc tctttcgccc tggccacaaa cacagaccat
    32701 accatcagag tagccgccta cgatggaagc gtggaatccg agctcagccc gcctttgtat
    32761 atacccgctg tggccaacga cgatctggtg cacccccccc tcagcctcag cgtctatcca
    32821 aatcccttca acgccagcca ggacttgcgg atcaagcttt caagccagat cgacgctccc
    32881 ggcagcctca aaatctataa cctcagaggt caactgctga ggaccgtccc cttctccaaa
    32941 gaaacggcat cagagcttag ctggaacggc cgcgacgact ccggccggga ttgcgggacg
    33001 ggcatctatc tgctccggat ccaaatcccc ggcatgaagg ccatcggcag gcgcatcgtc
    33061 aagctctgag gctgcttagc cagcttaacg tcacctgggc ctatcgcacg cccctcctat
    33121 gcttatgcat gggataggcg tgcgataagc acgggatagg catgggaggc taaagggaaa
    33181 ctgctgcgcg agcagcatat atatggctta tctttggttt gggagctgtg tgcaatggtc
    33241 tttgggtgtg gtcgtccctt ccttgcggtc agaagtgaac cggtaaaaaa aactcgtgtc
    33301 aacctgatga atgttgaaaa ttgtgcccgc atggaacgct ccttgcaata ttgggggtac
    33361 aagaatatca atcctcatca aggagccacg tatgcaatcc agcttcatgg ggaaagcggt
    33421 cctgatcata tcggtcctgc tttccttctg cctcatcctg tctgcccaaa gccggcagat
    33481 cgatctttcc acccaaagca atcagaccct gctcaagaac aacagcgatc tgggctttga
    33541 agtcagttat agtatcagtc agctcagcct gcgggaagtg aacacccgcg ctggcagttt
    33601 tgatgagctc agcatcgcag gctataccca cacaaacgcg atcggcgaac ccaagctgcc
    33661 catgcaaaga cagctgatcg ccgttcccct gggagccgaa gttcgctata gcatcctgtc
    33721 cagcagcacc cgcgatctgg atgccaaaga cagccagctg cgccatcgga tcattcccgc
    33781 tcaggagccg gtttccaaat cggcggaccc ctccacgctt cccttcatcc tcaaggaaga
    33841 agcctatcag aaggctgatt atcgctccag agaggtcgtc cgcgtggaag acctgggttt
    33901 tatgcgcggg atcaggcttt ttgccatcga cttttatccc gtcagctatg acccgctgca
    33961 aaactccctc acagtgctgg aaagcgctga gatcagaata gactttatca atcccgacct
    34021 catcggcaca gctgaactgc tggccagaac aggctcggtg gaatatgacc agctctatgc
    34081 cagttccatt ttcaactgga acaccgatac ccgcaccagt cttgtgcgct atcccaccaa
    34141 gatgctcatc ctctgccctc ccaactatac ggacgagatg cagagctttg tggattggaa
    34201 aaagcaacag ggctacaacg tgatcgttac cacagtagga accggcggaa cggtagccaa
    34261 taccaccact gccataaaca cctacatggc aagcgtctgg agtgccgcga cggcgcaaga
    34321 tcccgccccc acctatctgt tgatcgtggg agatacttct acctccggtg acaacatcat
    34381 tgccaataca ggcgtcacat ccagtcacgt gaccgacctc acgtatgtgc gtctgcaggg
    34441 caccgattat gtgccagaga tgtattatgg ccgcttctca gtctcctcag ccacagagct
    34501 caccaacatc atcaacaaga ccctcacttt cgaaaagact cagatgaccg acctcagcta
    34561 tctgggaaaa gccgtgatga ttgccggaca ggacggcacc tttggtccca ctcacggcaa
    34621 cggggccatc aattatggca ccacacaata cttcaacgcc agcaatggcg tcacttccaa
    34681 cacctatctc cacaatatct ccggcaccag cgatgccgcc attatcgcca atgccaatga
    34741 aggccgcggc tacatcaatt acactgctca tggcagtgaa accagctggg ctgatcccac
    34801 ttttagcgtt tcggatgtaa actccatgat caatgcggga aagtatggcg tcatggtagg
    34861 caactgctgc gtcaccaatg cttttggcac cggtgtgtgc tttggcgaat ccatcatccg
    34921 caaagccaac gcgggtggag tggcctatat tggcggcacc aacaacacct attgggatga
    34981 agactactgg tgggcagtcg gtgcgaaggg aaccgccaat ggcacagctc cagcctatga
    35041 cgccaccaaa ctgggtgctt atgacgccat gttccacacc cactcagaag cccagacgga
    35101 ctgggcccag accacaggag acgccatctt gatgggcaac ctggcggtcg cacaaggggc
    35161 cagctcccgc acaaactatt actgggaaat atattccatc atgggcgatc catctctgat
    35221 gccatatttc aaggtcccga gtgtcaacac ggctaccttc cccgccacca ttctgatcgg
    35281 cgcgacttcc atcaatatca ccgccgctcc ttattccaga gtgggactca gcatgggcgg
    35341 aactctctac gggactggga ttgttcccgc atcgggaagc ctgacgctta cgatcactcc
    35401 ctttgcgtcc accggcaccg ccactctggt gatcaccgcc caaaacaagg ttacccggat
    35461 agagagcatc accatcgctc ccaacagcgg tccctatgtg aacgtgaccg ccagcaacta
    35521 caacgacagc aacaacaacc agccggaata caacgaaacc ggacgtttca gtcccgtttt
    35581 ccagaatgtg ggttccgtgg ccgccaccaa cgtgagtgct actctcactt gttccacccc
    35641 tggaatcacg atcacggatg gagctcacag cattgcctcc cttgccgctg gagcttccag
    35701 caccgccacc aacgcctaca gctttacgat tgccaacaac atcgccaacg gcactgtggc
    35761 gcagtttacc atcacttcca ccatgagtgg ccagaccccc tgggtgtata acttcagcct
    35821 caccatcaac gctcccgcgc tggcttttgg caatatcacc atccaggacc cctccggcaa
    35881 caacaacgga cgcctggacc caggtgaaac cgtgacttta gtcatgccac tcaacaacac
    35941 aggcctggct gcctctccag cgggaagcgc gacgcttagc tgctccactc ctggaatcac
    36001 tgtcaacaca ggcacagcca gctttgcagc gatttccgca tctggaactg ccagcctcag
    36061 cttcagcatc acagccgctt cctctatgac cataggcaca gtggccagcc tggtattcaa
    36121 cgccacggcc ggttcctata ccgccaataa aaccgaaaac actgccgtgg gcctcatcct
    36181 ggaagacttt gagactggta actttagctc ctatccctgg attctgggcg gcactttgcc
    36241 atggacggtg gccaacacag gagctcacgc gggcacctat accgccaaaa gcggtgccat
    36301 cactcacagc cagtccagca ccatggaaac cacccgcatt ctcagcacct ctggtaacct
    36361 aagtttctgg tataaagtct cctccgagag tggatatgac tacctgaagt tctatgtgga
    36421 cggcgtgatg caaaaccagt gggcaggtac agtggattgg actcaggctt cttatactct
    36481 ggctgccggg acccgggtgc tcaaatggga atacatgaaa gacggcagcg tggacagcgg
    36541 atcaaactgc gcctgggtgg atgacatcat cttccccgcc tccacagctc ccagcaactt
    36601 ctatccccct caaaaccttg cagcgactcc gggaaatggt tttgtcaacc tggcctggca
    36661 agcccctcag accggaactc ccaccggcta caagatattc cgcaacagcg ctttgctcac
    36721 caccgtgacc ggactcagct atacggataa cgccgtagtc aatgagacta cctacagcta
    36781 ttatctcaag gctgcttaca gtggcgggga ttccgatccc accgcaaccg tgacggccat
    36841 gcctaccgct attgtgccta cgtcagttat cctgggtacc ggaacagcct caacgggtac
    36901 atcagaaggc gcgcctatca acatttggta caagagcctg cacggtcaat cggtttacac
    36961 ctcggcagag ctaaacgcag ccggcataac tggcccgaga agcattactc aaattggctt
    37021 caatattact gcgttaccgg ctctcgcgat gcccaacttt gttgtgagaa taggtcatac
    37081 tactgccagc aacgctgcaa gctggacctc gacaggcctg actcaggtgt ggagctcgac
    37141 ttcttatctc ccaactacaa ccggctggaa catgtacact cttactactc cgttcctatg
    37201 gaatggtacg ggcaacatcg tgatagacac agcctttggc ctgctggcaa attacaacca
    37261 atccggcact gtccagatat ctaccatgac cagtggctac atatttggcc gcagcgatac
    37321 aattgatcaa accaacctct tcaccggtac aagcacatcg acatacagac cgaatgtgaa
    37381 aatggtgttc cagcccaatg ctgatggccc ctcaatcgcc gtcagtccaa gttctctggc
    37441 ctttggagag gtagcagtgg gaggcagcag cgttcagcag ttcaccatcc t
//
LOCUS       DAOSFN010000006        34970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_968_length_34970_cov_9.465101, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000006 DAOSFN010000000
VERSION     DAOSFN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 34970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34970
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_968_length_34970_cov_9.465101"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1363)
                     /locus_tag="PLO35_01015"
     CDS             complement(23..1363)
                     /locus_tag="PLO35_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH78270.1"
                     /translation="MFSQGPSTINTLTGTRDNIARGYLPVYENHFLNWDSYRYTPVSD
                     SFGKMLVICHTNYMSTILPYVNWKKQKGIDTELIQWSSIGTTAAQLQTYIQNRYNADN
                     TITYIQLVGDAPQIPSLSSGGGGSDPTFALVAGSDNYPDIFIGRFSAETTDQVTAQIN
                     KAIAYERDVTTADTWLSRALGIASAEGGGSQGDNGESDIVHMNGIRTDLLGYGYTSVD
                     QVYDPGASASTVTTNVNAGRGFINYVGHGADTYWVTTGFSNTNASALTNGSKTPVIMD
                     VACVNGNFVSMTCFAEAWQRNANGGSVAIYASTINQSWNSPMRAQDHFTDLMAAGSKL
                     TVGGLYYNASCDMMDAYGSDGVNMFKTWHIFGDASLLMRSKTPMAMTVTHPSNLTSGT
                     TSINVSSGVANTLIAITYNNTIYGRAYTNSSGAATITLNSPPSGALKYTSRSGS"
     gene            complement(1438..1920)
                     /locus_tag="PLO35_01020"
     CDS             complement(1438..1920)
                     /locus_tag="PLO35_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HOH78271.1"
                     /translation="MRKITLAILFILLTLSLFSEQVILGSGENSITVLTQSEAETILQ
                     YSIENFVKEPVVIDNATWYHVTLPKEGTTQEQGFPQLPVFNRSIVIPNLSAMDYQVYD
                     IKYQDLILPVAPSKGVITRDTNPATVPYTFDPVYRGKGFYPEKIATLSDPYILRDFEE
                     "
     gene            complement(1947..3269)
                     /gene="ffh"
                     /locus_tag="PLO35_01025"
     CDS             complement(1947..3269)
                     /gene="ffh"
                     /locus_tag="PLO35_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOH78272.1"
                     /translation="MFSNLSEKLDKIFRNLKGRGYLTEQNIKDSMREIRLALLEADVN
                     FKIVKNFVLEVEKRALGAEVMKSLTPGQQVVKIVYEQLCSLMDTEGFEFKVYDNKLIK
                     VMMVGLQGSGKTTACAKLALMFRKKNIKPMLAACDVYRPAAIEQLKVLGKQIDIPVVS
                     VDSKNVIEIADAALREAQKDFSNLLIFDTAGRLHIDEVMMDEVARLKAHVKPDYIFFV
                     ADAMTGQDAVTVAKEFYNKLSFDAVILTKLDGDARGGAALSIKAVTERPVAFVGTGEK
                     VSDLEVFHAERMASRILGMGDVLSLIERAEETLDAEAEEKLARKMLKNQFTLNDFLKQ
                     LQSIKKMGSLESIIRMIPGLGHKLPADMKIDENALRHVEAIIQSMTEKEREKPDILNG
                     SRRLRISKGCGRSVTEVNRLLRQFEDMKKMMKRFSTPQGMKDLEKMMK"
     gene            complement(3309..3959)
                     /gene="rpe"
                     /locus_tag="PLO35_01030"
     CDS             complement(3309..3959)
                     /gene="rpe"
                     /locus_tag="PLO35_01030"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOH78273.1"
                     /translation="MKKIYIGPSLLSADFSRLGEELTALEAAGADFIHLDLMDGHYVP
                     NLSFGFPVISILRKHTKLPLDAHLMVSNPADYVDRLADLGVTGITFHQDSETHSHRLL
                     RRVRDLGLKAGLALNPSVGLETLRWVLPELDYVLLMSVNPGYSGQSFIPAILSKTREL
                     KTMIDTSGCPVHIEVDGGISAANSSTLVSAGADMLVSASYIFNSPDYATAIASLRG"
     gene            complement(3969..4274)
                     /locus_tag="PLO35_01035"
     CDS             complement(3969..4274)
                     /locus_tag="PLO35_01035"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOH78274.1"
                     /translation="MNHPGRMALNSISTIGHRLALNLAGEKYRPFIKLYLGWRAVVGD
                     LLAQRSHPYRYKDGILYVAVHNNSWLQELVLRKQEIIAKCAQECGETPKDMMFFIRA"
     gene            complement(4343..4915)
                     /locus_tag="PLO35_01040"
     CDS             complement(4343..4915)
                     /locus_tag="PLO35_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78275.1"
                     /translation="MRDTWFNIKQIAALVVFIIAFSLMGMMTGRPIMVLLYAVVLALA
                     TGITYLIIRKRQRHSEISLQENRLPKLIIGIILCVLAIATPLYLISKTSLINIPGNIT
                     IMVILAILGVTLLFIALFALAVYLINHKGDELPMRVIGYVVIVVTALIPGLLMSMYDK
                     TASTIGAVYYVALAVLILAYNGINMVLRRP"
     gene            complement(4931..5494)
                     /gene="rplI"
                     /locus_tag="PLO35_01045"
     CDS             complement(4931..5494)
                     /gene="rplI"
                     /locus_tag="PLO35_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOH78276.1"
                     /translation="MKVILLNNIEKLGSQAEIVNVKRGYARNYLIPRDMAIYATPQNM
                     KRLGDLKAKAAVEEEKRLTELKKLAGKINSVNLVFIRKVDENEHMFGSVSEADIVTAL
                     AENGIEIHKSAVQLEKHIKELGHSQVQIKLHKDLSAVVKIDVHKEAQEEIVTMAEVQE
                     PELPAEQADEPEADSPADETPDPTDSI"
     gene            complement(5491..6327)
                     /locus_tag="PLO35_01050"
     CDS             complement(5491..6327)
                     /locus_tag="PLO35_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78277.1"
                     /translation="MFYPLLLGALAISFPFLSLVGMMIFSGRAALQGIDVPIRVIAAF
                     FLAPAALMLWDHSGMLIPALDAILGVGLVCLIFLLALKARLPFGTAFTLGVLAIIAYG
                     LVRVLAWGEQLSAMHIEALEVAMDQMRQMLDKTMLQTTVDLMGSFWPASWMLSQIVAL
                     FAGYLIFQRSMGIGFRWGDQRFPSAYNFLLMAILPVYFIPTARMLFINGFIALCVIPF
                     IQGLGLLIDRLGRIVQNRLIRIFVLVFLLINIVSYALITLFGFADLWWNFRNIKTGGN
                     PA"
     gene            complement(6330..6566)
                     /gene="rpsR"
                     /locus_tag="PLO35_01055"
     CDS             complement(6330..6566)
                     /gene="rpsR"
                     /locus_tag="PLO35_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOH78278.1"
                     /translation="MNITDRKKKFARRKYCRFCANKELVIDYKNIDMIRQFISDVGKI
                     DPARITGTCAKHQRKLTAEIKRARQMALVPYVIE"
     gene            complement(6579..7016)
                     /locus_tag="PLO35_01060"
     CDS             complement(6579..7016)
                     /locus_tag="PLO35_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH78279.1"
                     /translation="MADLKLPRLNKIFVSGRITQELELKYTPKGTPVLRFTLANDKAF
                     KDESGQWQNQAIYLEVVAWNNQAENLIKMAQKGTAVLVEGRLDARTYVDQNNQNRKIY
                     EIVADHIQTLEWKPREDGVTEQDIPLPEEPQHAPTATKDDVPF"
     gene            complement(7027..7317)
                     /gene="rpsF"
                     /locus_tag="PLO35_01065"
     CDS             complement(7027..7317)
                     /gene="rpsF"
                     /locus_tag="PLO35_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOH78280.1"
                     /translation="MIKNYELMVIVSPKLNGDEADAANEAILNLVRENGGEVVKTDPW
                     GRKMLAYPINKMQEGYYYVNYFSLESTTVKVLKRLMNINENILRHMFVAKGE"
     gene            complement(7328..7939)
                     /gene="pth"
                     /locus_tag="PLO35_01070"
     CDS             complement(7328..7939)
                     /gene="pth"
                     /locus_tag="PLO35_01070"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOH78281.1"
                     /translation="MKLILGLGNPGPRYQNTRHNLGYLCLEEWSRAHKRSFQSDKLYD
                     YISFKNAVLIKPTTWMNLSGEAARAATRRWDISESLVVYDDLELPLASLRVRGGGGDG
                     GHNGMKSLFEVLPADDLKRLRLGIGKDQSVDAADYVLDELTAEELLAIKPVISKATEL
                     IDIYINRDFTAVLNEYSKWMKSYSASGVRDAETPPDRNGIISP"
     gene            complement(7949..8554)
                     /locus_tag="PLO35_01075"
     CDS             complement(7949..8554)
                     /locus_tag="PLO35_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOH78282.1"
                     /translation="MIFTLEAQTRKTVRKSDLTKLRAQGMIPAVLYGPNMDSLSISID
                     QAEFTRCYKKSFNELAFYELQLAGVKYHTILKDKLVHPVGRHILHIDFMVVPATAQME
                     FEVPIHFNGEAVGTHEGGFVDVVHRTVKLVCQADVVPDGLDLDISNLRVGESLHVSDL
                     PKGPWTIKDHEEVTLVVIHAKKAEAVAEPKAEPAAKPAEEK"
     gene            complement(8728..9672)
                     /locus_tag="PLO35_01080"
     CDS             complement(8728..9672)
                     /locus_tag="PLO35_01080"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOH78283.1"
                     /translation="MYAKLKLITGNANRPLAEEVARYAGIPLADIDLFKFSNDESFVK
                     INDNVRGADVFVIQPTCRPVNDNLMDLLIIIDALKRASAQRINCVIPYYAYARSDKKD
                     QPRVPITAKLVADLITVAGADRVITVDLHADQIQGFFNIPVDHLFSIPSFARYFQSMK
                     MDNVVVVSPDSGGAIRARALAKRLDCGLAIGDKRRSGNNDQTELLNIIGDVAGKTAIL
                     FDDIIDTGGSLIKVAHALHKQGTQRIFAACTHGVLSGSAVENLEASPIEKLFVTNTIP
                     LVEEKANCQKIVQLSIAEMLAVAIKKIHIEESLSVLFR"
     gene            complement(9651..10502)
                     /gene="ispE"
                     /locus_tag="PLO35_01085"
     CDS             complement(9651..10502)
                     /gene="ispE"
                     /locus_tag="PLO35_01085"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOH78284.1"
                     /translation="MPAASYAKINLSLEVLGKLPDGYHRVETLFASVDLYDTLKYTLT
                     KNPGIKLWSDVSELSTPDNLIYQVAEHLRQAFRPGAGARIELFKRIPIAAGLGGGSSN
                     AAITILALNRLWELRLSEEKLDEVAARFGSDINFFLQGGCCLGEERGQRITPMDDVLI
                     DNILLVNPGLRISSAEAYKLVDLSSVSQRGSYQDFFRSGVCFNRLEPGIRSRYPLIDE
                     IIRKMTEAGARTAVMSGSGSTCFGIFDSNSALQTCRQAFEAKGFWTHKTRTIGKEEYQ
                     KCTQSLN"
     gene            complement(10462..12750)
                     /locus_tag="PLO35_01090"
     CDS             complement(10462..12750)
                     /locus_tag="PLO35_01090"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOH78285.1"
                     /translation="MEQQNLPPQPQDEIKLSDYLRIILQFRYLIVLVFVIVMALTIVY
                     TARQPRVYSASNRVLLENQSNSADLLFLTTPGAGKNTINNQIELIRSKPILTAAWEIM
                     KKYPEWESFPAAASANPAGRLAGVKVESKRDTDILTLSFESTNQGEAMAAVNSIAEAV
                     QQQNTQYARLEFTTIREFLETQLDAISRRLQSSENDLREFKNMNKLTELSTETQKLIE
                     SSAEIEAEYEAALTDQAVKARTLDLYRRQLQEQDSLVVNVNSVLKTPYINELRKQVIE
                     TQSLITKLVTKNNYPLDHPQILALETELERTKTKLDLEIKKLVAAATTDDPLASRNET
                     LVKIIQTNVELEMARTRVDGLAQTKEMYDERIISLPNTELELARLMRNMQLDGKIHGI
                     MMEKYEDAKIAEQAKVGNIRIVDYAERPSAPIKPRVSMNILVGIILGLGLGVGAAFVV
                     HSLDTKLRTLEDMENFVRLPIVGTIPLIQESEARMEEFNAMIEKAEGENKEQLSKSMH
                     FVMMQLVSHYAPKSPIAESYRTLRTNILSRKPAGSTTLLVTSSGPKEGKSTTIVNLSI
                     TLAQMNARVVLVDMDMRRPMIHNKFGIEKENGLSDYLIDPEVSIEQVTKATGIPNLDV
                     ITSGFVPPNPSELISSTRTDTLIDELKDRYDFILFDTPPVIAVTDALILTKKVDLTFL
                     VVRCGFTEKGIIKRTKELMDNISSTIDGIIVNSIYVQKYYNKQNYYYYYYYYYYYYGE
                     ESPKKVKKKNAGGFLRKNKPVA"
     gene            complement(12764..13255)
                     /locus_tag="PLO35_01095"
     CDS             complement(12764..13255)
                     /locus_tag="PLO35_01095"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOH78286.1"
                     /translation="MNKSIFIILLLAMGCALFAQTASPTTNTGGISAYTYDGARTGVE
                     KLKMNVYVLGQIYKPGLYVVPDDTNFITLLALAGGPKEDAKLTKIRIIRPKGVDEKPE
                     IIWVNLKKYMETADENLLPMMMPGDTVVLSGTIFYAFTKVADFLSKVAIALSVYNLVT
                     NLK"
     gene            complement(13397..13744)
                     /locus_tag="PLO35_01100"
     CDS             complement(13397..13744)
                     /locus_tag="PLO35_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HOH78287.1"
                     /translation="MIQYIIKTFISLAVIVGVSEISKRSTLIGGILVSLPIVSILAMI
                     WLWIDTRDQARVAQLSTSVFWLVLPSLTLFAILPLLMKRMAFGYALLISCAVTVLAYY
                     LMVLILGRFNIRL"
     gene            14429..14914
                     /locus_tag="PLO35_01105"
     CDS             14429..14914
                     /locus_tag="PLO35_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78288.1"
                     /translation="MLISIISIISGSCAFNRIDIVPRYRLVSNIQFAIQDHLVLVFES
                     ADKQPVIVLYPVDKKLSAEFTVRQIPKGGKIKLNLEEIPDSLRFERFMFPKTSRVDPN
                     NYEDMVFEIGHRGKVYAYFDTKTGQLLGKCYYASNLFVLDYLSPLLFEIAPAGKYPIQ
                     K"
     gene            15330..16853
                     /gene="proS"
                     /locus_tag="PLO35_01110"
     CDS             15330..16853
                     /gene="proS"
                     /locus_tag="PLO35_01110"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOH78289.1"
                     /translation="MSKNKRTAIEPTREENYAEWYQQVIRAADLAENSDVRGCMVIKP
                     WGYAIWENIQRVLDQMFRETGHRNAYFPIFIPKSYFEKEAEHVEGFAKECAIVTHSRL
                     EPDGNGGLKVAGELTEPYIVRPTSETIIGASFSKWVNSYRDLPLLINQWANIVRWEMR
                     TRLFLRTAEFLWQEGHTVHETETEAMDETMKMLDVYARFASDYLAIPVIKGEKTEGEK
                     FPGAVSTYCIEAMMQDKKALQSGTSHFLGQNFAKASNIRYQNRAGEFDYAWTTSWGVS
                     TRLIGALIMAHSDDNGLVLPPKIAPSHIAIVPIYRDDQEKAAVLDMANQIVEMFRGAM
                     LDDIRIYTELDDRDITSGERNWYWIKKGIPLRIEIGPRDVASQAVMLARRDQEPRDKK
                     SVPLVELREHAIQAMLDMQASIYQNAVAFRKANTVRIDSRDEFYSYFTPKNAEQPEIH
                     GGFALSHWCGDVACEEQIKDDLKVTVRCIPFDGEAEDGNCVCCGKASHKRVIFAKAY"
     gene            16837..18090
                     /locus_tag="PLO35_01115"
     CDS             16837..18090
                     /locus_tag="PLO35_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOH78290.1"
                     /translation="MPKPIDLKPVSSAVISIGNEILLGKTVNTNLAYLAAGLAALGAP
                     VLYSVTIRDDADEITRALEYCTGRYDIVISTGGLGPTADDITKATIASFFGKELVFHE
                     DIWEFVQSLFARRGLKAPEINRCQAMVPQDFVALENQMGTAPGLQYEWDGKCFFALAG
                     VPVEMRHVFDNHISSIIHARYPHLSKVIQRSIHTFGISESALAERLDTSLIPKDLGFA
                     WLPQTGRVDLRVYGHDENMINDTADKLIAQIPELVWGIDEDTPAGVLGRLLRDKNLTI
                     SLAESCSGGLVSKILSDEAGASDYLLGGVVSYHNQLKHNILGVSETSLAEHGAVSEET
                     ALEMVTGIKRLTDSACAISVTGIAGPSGGSDSKPVGTVYFGFSYLAEVWTQRHVFTGS
                     REIIRHKAAEMAILTLAKKIMGRDQ"
     gene            18087..19361
                     /gene="purD"
                     /locus_tag="PLO35_01120"
     CDS             18087..19361
                     /gene="purD"
                     /locus_tag="PLO35_01120"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOH78291.1"
                     /translation="MRVLVIGSGGREHAIASAFARSSKVSNVLVAPGNAGIAKDFDTL
                     PLSTLQEQIDYCATYRPDLVFVGPEQPLERGLADRLRELDIPCVGPSQAAARIETSKI
                     FAKQLMQKYSIPTAAFFHTFSLDEALSYCAVDCKYPAVIKADGLAAGKGVIIVRDAIE
                     ARSALETLMGVQTDGRAYNSPSKPQKGVVIEEYLSGWEVSLFAITDSQDYVTTLFSQD
                     HKQLLDHDAGPNTGGMGAYAPVPEAEPYRSEIESRIISPVLAALRDEGCPFSGILYCG
                     LMITAEGAKVIEFNCRFGDPETQALLPLLDTDLVEVCQAIVTGQVRDLKLHWRDQSCV
                     AVVMASGGYPGNFAKGYVLKIPASLESRLFYSGVESKDDMLVSSGGRVLCVSATGPDK
                     DSARAQAYRDIDQIHFQNMHFRKDIGLRKNDL"
     gene            19460..24484
                     /locus_tag="PLO35_01125"
     CDS             19460..24484
                     /locus_tag="PLO35_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH78292.1"
                     /translation="MKLHIWLPLLLVLPLALSATGISVSSETPDELILEFQLPEYSIE
                     HSRDGSTTWQRIVSDYGNVDAQEGYPELRAYSEAIAVPVDGDISIQVLSSKSSVIKNI
                     NLYPAKKMVVGDWEVDYEFFQDPRIYNSKQLYPESIASKSEAAFIGDRRFIPIRINPF
                     QYRPALKELTVHSSIRIRVSIIGDKSQAKNWQFSQNPIDNVADRFFLNNASSKAWRLE
                     KTRDGSYQSPKNGSSQVSRIQLIVDKDGIYKVDKAFLQNLISTETDSLGLTMAWDLSA
                     LDPRRLELSDENGTVPIHFVGETDGAFNSGDYFEFYGRRHAGDTGYMDDYTAENVYTL
                     YLKDTLGARMAVENGGLIEYSSGNYISPDAYEYTAIFQEQSVSDKLGRGWSAANPTYY
                     KEDVWFWKKISAPNLEIVPIELQFPIDSTIRTASAKVALHGLTYSETLIPGQWDHEAS
                     VRLNQAMINTHTWVGQTEQVFQNANPIPNSFIRNGTNNLFISLSGNTVMGDREQVLLD
                     YVEVKYWRQYKTTEDFIKFTKPSNRPGGLYQFALEGFSSGDISVYKIGSSVFNSVQIE
                     SFDLNGMAPWTVTMQDSVASTAIEYYACTESQKMIPKLARLDIPSDLRDPSNQAEIIV
                     ISRRDLLNSEGGTRIKDLYESKGYTVMMVDIQDVFDEFNSGIRDAQSIKSLLSYAYNN
                     WAEPQLRHVLLLGEGVDDERDNSPSRQYAVIPVKKTWTYKHGATASDNWYACIVGNDT
                     VPDISIARVGVWRTDQIPAVVTKMENYHNNPLPNRLWNSHLTFTSGGKITDSNDIFAQ
                     QSERIKRRNVSDDYRVTRVYTSTQTVSPDYFGGTFALKDAINSGTQYVQFMGHGGGRI
                     WADYNLFNFNDVATLNNQTYPIVVSLACYASAFDTNGSASISEALIMQADKGSIATLG
                     FSGLGYLDQDEDWGLALTEALFKEDFPSAGEAVQFALARFFTTTTSTAARYALTNGAS
                     YMGDPTIKPRKPVRDAQVGAVSHVLAHGDTLRVNAVFPNTVTAARLYIMKENEKVINV
                     PFDLPVINGNYNASYVVPATGEDPYTRKIYVAGYSSQNEYVGVSAFGVGRPAVFHYAI
                     EPATPAWRDSVAFMARAFSSTPILSMTCKVRTDSVGTTINWVDLPMQLTDPDNNTWTT
                     TGRLDPQITGKEVFYKYSISTQDSTGTVVYESFLSNLVYAGPDLFLRDIKLDNEDGEF
                     ALKVLVSNVGNAESADTDLRLYVSINGANQTQFSTQVLGPLAVEEMRWESIPLAGLPS
                     GNVNFEVRANWGNSFPEWHLFYNTNNIITLTLPYNYHSVDSNGLVIPSVDANVLCEIP
                     ADLVPSGSSAVFYVNSVGTLTASNQPDIHPIKLLSSDGAANPVSSLAYEIKTLNEALV
                     DSTGTFVGGKRMKLTFFYSALDQTTQSQEAENSYKIYRWDASGRKWILQGGNISASED
                     RVVFEVRRHGIYTIFRNRDLIRPTIDVNVQDQEFTIGGYVSGKGTISLLLSDANGIDV
                     FDNSIKLYLNGTQVQDNEYVASINLDNINRIPIKYQLNLQRGNYTLVVDCKDVNGNFN
                     TREIQFIVNETFNITKIGNYPNPILGRAEDPKNDGRTRFTYVLTDDADQVTIKVYTVA
                     GRLVKTFTNLPTGVGYHEYPRTLYAWDCKDEAGFPLANGVYFYKVIARKGNKTIEKTM
                     KMAILK"
     gene            24493..25587
                     /locus_tag="PLO35_01130"
     CDS             24493..25587
                     /locus_tag="PLO35_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78293.1"
                     /translation="MKQQFIITEQDERAEMTGCHSPRTLPPFHAPRLLLIFLACVSIL
                     SLSAGRYAGDFMMIGAGVRPLGMGGAFSALADDGSAIFWNASGIAGITEREVAAMHAF
                     LYGGLASYDNLSYIQPLPNGVSIGLNLTRLTVSDIPYFSESYLIGTNVDQRINNSAYH
                     LPGVPDSEFDSTDDLYQFAFAKHFRYDANMGWLFFEIPFDFYLGGNVKFIKRKLRTNY
                     GTGTGVDLSFKITTDMGVIFDVEELGKLDFAMNFQDVAGTDIAWDTVNKIKDEVLFNT
                     KLGLAIRQPIPPLKSELKLAYDYDYVYEGTSHYGVEWDYNNKGSLRAGYYDKNISLGA
                     SVKIYDVNLDYALVTNPVGVTNRLGLRLRF"
     gene            25602..26369
                     /locus_tag="PLO35_01135"
     CDS             25602..26369
                     /locus_tag="PLO35_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78294.1"
                     /translation="MSKTLQLIALLMLTVILISCSGTDKPEEDTTPPFPPTMVPHLGD
                     VGDPPTTYYGQSAILTEDNNGIDTVADGNWIRIAWKPFIDEDLNLVRIWRFDEFNPTP
                     VQIDSIAPNNDYYLDSRDQLPERIWYSYFIDIVDFAGNTARSDTVSYALLAKPVLLDP
                     PNGATISPVGARVYWNSSGFASIYRLVLFDSNHNYLWHQDLPTAMEEQLFMDIPVNLL
                     TQHSGEGIYWRIDSFDWDEERQQYLGAESLERIVYIQ"
     gene            26634..30428
                     /locus_tag="PLO35_01140"
     CDS             26634..30428
                     /locus_tag="PLO35_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH78295.1"
                     /translation="MKKHFILLMLLSLAALLMAAQGSIVFSQNPSSVQLLREAQNGLS
                     VRYTLDKLDYQDMDTPEGRFTDLSVQHYTSTNVAGLPKLPLQRQIIAVPEGATVVPSI
                     VTAQRQIIALASRGINYPIMPRQESVSKSANPADISFEVNRDFYNRSAWTQEPFVRVE
                     EIGYMRGVRLFALDFMPVRYNPAQGEIEVIVEAEIRVDFAGADLAATEALRERYYSPA
                     FNGIYQKSLLNPLDNRATLNRYPMSYLIITPPNFVTALQPFVDWKIKEGFNVIVATTT
                     QTGTTANAIKTYLQNIWNNATTANPAPSYLLIVGDVAQVPSNTGTTGSHVTDLTYVRL
                     QGTDFVPEMYFGRFSATTEAQVTNQVNKTLMHEMYTMPSDAYLGDVVMIAGADPSWAP
                     THANGQINYGVNNYFNPAHGINSFTYMYPASGSSDSQIIQNVSNGVGYVNYTAHGSET
                     SWADPTFTVANVNGLQNVNECPVVVGNCCLTSAFNTNVCFAEAWLRAENKGGVIYIGG
                     TNSTYWDEDYWWGVGYKPPVVGSGSPFIAGRTGAYDSMFHEHNEPLADWATSVGAATF
                     MGNMAVVQSNSSRINYYWEIYSVMGDASLIPYLGIPALNSAVVPETIFLGMGSADIIA
                     DPYSYVAISMNGILHGTGLVDATGNMTLSFTPFTEPGTAQLVITRSMRKPLIANIQVI
                     PNSGPYVTVSPITVNDPNQNGLAEAGEIISMDISFNNVGVVEATGLTASLTTTNPWVS
                     LLTASSPIPNVPAGSSIDVTDLFSVQISPMIPDQQVINFDISITDGSNTWSSSRSITA
                     YAADLQITGVTMNDGNTNGFLEPGEVVTIQMALANNGHMPISGGSMVIVPNHPGVTLD
                     NESFVLPPMSIGASAQISVIAFLAGDLQTGTVISLGVAINAGAQMINHTISLPIGMTG
                     EDFEAGNFTNYPWTNNSPVPWTIQSGANNTHGGTYGAKSGTISHNGTTELSLTLNVGA
                     AGNISFWRKVSSESGYDFLRFLMNGTEMASWSGTQDWTQMSYPVQPGPNTFSWKYTKD
                     GSVSSGSDCGWVDDIIFPMAGSSNAAIFYSPNETISFTEVAAGSTVSADLVIRNLGNI
                     TMSGMITMPTGFVLSMNGTVLPPDYSYNLEAGLNQVFMVSYTAPTPAINLDDVIIVSS
                     NDANNPAVMIPITVSAVVANDDPVIPAVTKLEGNYPNPFNPETAIRFSTREAGPVRLS
                     IYNLKGQLVKSLLNSNLPAGNHRVIWNGKDESGRSVSSGIYMYRMEAAGYSKTLKMML
                     MK"
     gene            30655..34350
                     /locus_tag="PLO35_01145"
     CDS             30655..34350
                     /locus_tag="PLO35_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH78296.1"
                     /translation="MKHLILTLTLLLTLLGLTAEQVTVGPNQNQVRLVQSQSDNMVLE
                     LTLGHFNREAVQINGSTWYHIDLKKAGLTLESGLPQLPVMAGSVIIPNTARMELSTVL
                     SEYVDIPMQVAPSKGNLTRDIDPDSVPYSFSPFYSGTEAWPQSNSELTEPFILRDYRG
                     ITVRFVPFSYFPSTGTLRVYTKIQVALNNTGTDLTNSLSVPKNSYSEEFEGIYENMFL
                     NFQQAKYPSLAEQGRILVIKHNMFDTDIMPWVNWKRQNGYQVDVVDVSVAGPTANNIK
                     TYIQGQYDLNNGLMFVQIVGDAPQVPTLTSGGGGSDPSFALLAGGDSYPDIYVGRFSA
                     QTVTELQTQVTKAVEYERDRVAGNVWLQKAMGIASNEGGGSQGDMGESDQAHMELIRT
                     DLLGYGYTSVDQMYQTQGATAAQVSANLNQGRGFINYVGHGSDTSWVTTGFSNTHVNA
                     LTNDNMLPVIVSVACVNGNFTSQTCFAEAWLRAKNNSTGEPTGAVAMYASTVNQGWNP
                     PMRGQDEVTDLLVAEAKKTIGGLYFNGSSKMIEVYGTSGISEYKNWTIFGDASLMVRT
                     KDPMPLTANYNPILFMGMNSFTVDTIAGARVTLSANGTVYGHAIADLTGNAVVSIPTP
                     PVEPMDLTLTITAFNREAHIATVQVLPSTGPYVVVTGMTVTDNNNNQADFGETVNIGF
                     GLNNVGSGAAQGVVMNLSTTSPYLTVLSGQDSIGDIPAETTGGTVTGFTIQIAANAPD
                     QHVAPLDLLITLSDNTTYAYTRNLTVNAPNMQWGALLVDDSVGNNNGRVEAGETVTLS
                     FPINNLGGAQANDLISALAINNGTNVSDPVQTTFTALPAGGSVNMIYTVTFSSQIPVG
                     TTINMTAMVFSGMYSAMQTYTMNVGQVMEGFEGGFGGYDWVFANGNWTTVPGSYNGTT
                     AARSATISHNQNTSMSVTMVSQAAGNISFWKKVSSEQNYDYLKFYINNTLKNQWSGTN
                     DVWSQLTYPVNAGTNTYKWEYVKDSMVSSGDDCAWIDDIIFPSTGGTTGAPMISMDIT
                     SLDYGDILLGETPVLPVTINNVGDAVLIGTVETSAPFSIVDGGGNLLEQMNVVIQPSS
                     FLQFDLRFAPSAAGSFDGSLIINTDDSANPSIVITLSGTANPVANDDPILPAVTALKG
                     NYPNPFNPRTTISYSVKEQGPVSIGIFNLKGQLVKTLVSEAKAAGDHTVVWEGDDDNG
                     RQVSSGIYLYKMYSGKYTSTKKMIMMK"
BASE COUNT         8480 a         9041 c         9004 g         8445 t
ORIGIN      
        1 accgactgat gacggagcag attcaggagc ccgacctgct ggtgtatttc aaagcaccgg
       61 agggtgggct gttcagggtt attgtagcgg ccccgctgct gtttgtgtag gcgcgaccat
      121 agattgtatt gttatatgta atggcaatca gagtgttagc gacgcccgat gaaacgttga
      181 tagatgtagt accggaagtg aggttggatg gatgagtcac agtcatagcc atgggtgttt
      241 tagagcgcat gaggagagag gcgtcaccaa agatatgcca ggtcttgaac atattcacac
      301 catcagatcc ataagcatcc atcatatcgc aggaggcgtt gtaatagaga ccgcctaccg
      361 tgagcttaga acctgccgcc atgaggtcgg taaaatgatc ctgggctcgc atgggagaat
      421 tccatgattg attgatagta gaggcatata tggccacgga gccaccgttg gcattacgct
      481 gccaggcttc ggcaaagcag gtcatggaaa cgaaattccc gttcacgcag gccacgtcca
      541 tgatcacggg agtcttggaa ccgttggtaa gagcgctcgc gttggtgttg ctgaatccag
      601 tggtgaccca atatgtatca gcgccatggc ccacatagtt gatgaagccg cggccggcat
      661 tcacgttggt ggtcacagtg gatgcggagg cgcctggatc ataaacctgg tctacagaag
      721 tatatccata gcccaggaga tcggtacgga ttccattcat atgcacaatg tccgattctc
      781 cattgtctcc ctgactacca ccaccttccg cggaggcgat tcccagagct ctggatagcc
      841 aggtgtctgc cgtggtgaca tctctttcat aggcaatggc tttattgatc tgggccgtta
      901 cctgatctgt agtttccgca gaaaagcgtc cgataaagat atcgggatag ttgtccgatc
      961 cagccaccaa agcgaaggtg ggatctgatc cacctccacc ggaagataag gaaggaatct
     1021 ggggtgcgtc tcccaccaat tggatgtaag tgatagtgtt atcagcatta tagcggtttt
     1081 ggatataggt ttgcagctgg gccgcggtgg tcccaatgga cgaccactgg atcagttcag
     1141 tatcgatccc cttctgtttt ttccagttca cgtagggcag gatggtgctc atgtaattgg
     1201 tatgacaaat caccaacatc ttgccaaagc tgtcgctgac aggggtgtaa cggtagctgt
     1261 cccagtttag aaaatggttt tcatagacag gtaaatagcc tctggcgatg ttatctctgg
     1321 tcccggtcaa agtgttgata gtactgggcc cctggctgaa cactctgatc ttgaaagcag
     1381 tgtatatcct cagggtctgg gtgaagggat cataggcaaa ggggctagtc tggatggtta
     1441 ttcctcaaaa tccctcagga tgtagggatc agacagcgtg gcgatcttct caggatagaa
     1501 gcccttgcct cgatagacgg gatcgaaggt atagggtacg gtggccggat tggtatcacg
     1561 ggtgattact cctttggatg gagctaccgg caggatgagg tcctgatact tgatatcata
     1621 tacctgataa tccatggcgg acaggttggg aatcacgata ctgcggttaa aaaccggcaa
     1681 ttgaggaaag ccttgttcct gagtggtgcc ttctttgggt agcgtgacat ggtaccaggt
     1741 ggcattatca atcacaaccg gttctttgac aaagttttca atgctgtatt gcagaatagt
     1801 ctcagcttca gattgggtaa gcacggtgat agaattttca ccgcttccca aaatcacttg
     1861 ctcagaaaac aaagagagag tcaacaggat aaacaggatt gccagagtaa tctttctcat
     1921 ggagtttcac atacatctat gtattatcat ttcatcattt tttccaggtc tttcatcccc
     1981 tgcggggtgg aaaaccgctt catcatcttt ttcatgtctt caaactggcg cagcaggcga
     2041 ttgacttctg tgacgctgcg tccgcagcct ttggaaatac gcaggcggcg gcttccgttc
     2101 aggatatcag gcttctcacg ctctttctcg gtcatggact ggatgatcgc ttccacgtgt
     2161 ctgagagcat tctcatcgat cttcatatcg gcgggcagct tatggcccag tccggggatc
     2221 atgcgaatga tcgattccag tgagcccatt ttcttgatgc tttgcagctg tttgagaaag
     2281 tcattgaggg taaactggtt cttgagcatc tttcgggcca gtttctcttc ggcttccgcg
     2341 tccagagttt cttcggcgcg ctcgatgagg gaaagtacat cgcccattcc caggatgcgg
     2401 gaagccatgc gttcggcatg aaagacttcc agatcgctta ccttttcacc ggtacctaca
     2461 aaggccaccg gcctttccgt gacggctttg atggataggg cggcacctcc tctggcatcg
     2521 ccatccagct tagtgagaat gacggcatcg aaggatagtt tattgtaaaa ctctttggcc
     2581 accgtaactg cgtcctgacc ggtcatggca tccgcgacaa agaaaatgta atctggcttc
     2641 acatgagctt tcagcctcgc cacttcgtcc atcatcactt catctatgtg caggcgtccg
     2701 gctgtgtcga aaatcaaaag gttcgagaaa tctttctgag cttccctgag ggccgcgtcc
     2761 gcgatctcga tgacgttttt tgaatccacg ctcaccacgg ggatgtctat ctgcttgccc
     2821 agcactttta gttgctcgat agccgcggga cggtagacat cgcaggctgc cagcatgggt
     2881 ttgatgtttt tcttgcgaaa catgagtgcc agcttggcgc aagcggtggt tttaccagag
     2941 ccctgcaggc ctaccatcat cactttgatc agcttgttgt catagacttt gaactcaaaa
     3001 ccctcggtat ccatcaggga gcaaagctgt tcatacacga tcttgaccac ttgctgtccg
     3061 ggcgtgaggg atttcatcac ttcggcgccc aaagcccgct tctctacttc cagcacgaag
     3121 ttcttcacga tcttaaagtt gacgtccgct tccagcaagg ccagccggat ctctcgcatg
     3181 ctgtctttga tgttctgctc tgtgaggtag ccgcgacctt taaggttgcg gaatatttta
     3241 tccagtttct cgctaagatt gctaaacata gttttatcct gtgggggaga ttggaatggg
     3301 cttggccctt agcctctcaa tgaggctatg gcagtggcat aatccgggct gttgaagatg
     3361 taggacgcgg agaccagcat atccgctccg gcgctgacca gggtggatga gtttgcggca
     3421 gagattcctc catccacttc aatatgaacc gggcagccgc tggtgtcgat catggttttt
     3481 aactccctgg tcttggagag gatggctggg ataaagcttt gtcccgaata gccgggattg
     3541 acactcatca acaatacgta atccaattca ggcagcaccc agcgcaaagt ttccagaccc
     3601 acggaaggat tcaaggccag ccctgccttc agacccagat cgcggacccg gcgcagcaac
     3661 cgatggctgt gagtttcgct gtcctgatga aaagtgatgc cggtcacacc caagtctgcc
     3721 agcctgtcta cgtagtcagc gggattgctg accatcaaat gggcgtccag aggcagcttt
     3781 gtatgctttc tgaggatgct gatcacggga aaaccgaaac tgaggttggg cacataatga
     3841 ccgtccatca ggtccagatg gataaaatcg gccccggccg cttcgagggc tgtcagttct
     3901 tctcccagac gggaaaaatc agccgataat agagatggac caatatagat ctttttcata
     3961 gtcatagctt aagccctgat aaaaaacatc atgtctttgg gagtctcgcc gcactcctga
     4021 gcgcatttgg ctatgatctc ctgtttgcgc aaaaccagct cctgcagcca ggagttgtta
     4081 tggacggcca catacaagat tccgtcctta taacgatagg gatgggaacg ctgggccagg
     4141 aggtctccga ccactgcccg ccagccaaga tagagcttga taaaggggcg gtacttctcg
     4201 ccggccagat tcaatgccag gcggtggcca attgtggata tgctgttcag agccatgcgg
     4261 cctgggtgat tcatccgccc tcacgaacgc cgcaaagcga tatatgaagg cggatgccaa
     4321 acacctgtat tattgtaggg cttcagggac gccgcaatac catattgatc ccgttgtagg
     4381 caaggatcaa cacggccagg gccacgtaat aaactgctcc aatggtactg gcagtcttat
     4441 catacatgga catcaacagc ccggggatca aagctgtcac aacaatcacc acatagccga
     4501 tcacccgcat ggggagttca tcacccttgt ggttgatcag atatacagcc agggcaaaca
     4561 gagcaatgaa cagcagcgtc acacccagga ttgccagaat caccatgata gtgatattgc
     4621 cagggatgtt gatcaagctg gttttgctga tcaggtacaa gggagtggca atcgcaagga
     4681 cacagaggat aatgccgatg atcagtttgg gcaggcgatt ttcctgcagg gatatttcac
     4741 tgtggcgctg gcgttttctg atgatcagat aagtgatgcc ggtggcaagt gccaatacca
     4801 ctgcgtagag cagcaccatt attggccgcc cggtcatcat acccatcagc gaaaacgcga
     4861 tgataaacac gaccagagct gctatctgct tgatattgaa ccatgtatcg cgcatggtac
     4921 ctcctggttg ttagatgcta tctgttggat caggtgtttc atcagccggg gagtcggctt
     4981 ccggttcatc cgcttgctca gcaggtaatt ccggttcttg gacttcagcc attgtcacga
     5041 tctcttcctg agcttcttta tggacatcga tcttcacaac ggcgctcaga tctttatgca
     5101 gtttgatctg cacctggctg tgacccagct ctttgatatg tttctcaagc tggaccgcgg
     5161 acttgtgaat ctcaatcccg ttttcggcca gagcggtgac aatgtcggcc tcagaaactg
     5221 aaccaaacat gtgttcattc tcatccactt tacggatgaa aaccaagttt acagagttga
     5281 ttttcccggc aagctttttc agctcggtaa ggcgtttttc ttcttccacg gcagccttgg
     5341 ctttgaggtc gccaagtctt ttcatgtttt gcggtgtggc gtaaatggcc atatctcttg
     5401 ggattaggta gtttctggca tagccgcgct tcacattcac gatctcggcc tggctaccca
     5461 gtttttctat attgtttagc aggataactt tcatgcggga tttcctccag ttttgatatt
     5521 tcgaaagttc caccacagat cggcaaagcc gaagagggtg attaaggcat atgatactat
     5581 attgattaac aggaagacaa gtacgaatat ccggattaag cggttttgta cgatccttcc
     5641 cagacgatcg atgagcaagc ccaggccctg gatgaacgga atcacgcaga gggcgatgaa
     5701 cccattgata aacagcatcc gggcagtggg gatgaaatat acgggcagga tagccatgag
     5761 caggaaattg taggctgacg ggaagcgttg gtcgccccat ctgaatccaa ttcccataga
     5821 gcgctgaaag atcagatagc ccgcaaaaag ggctacgatc tgagacagca tccatgaggc
     5881 cggccaaaat gaacccatca aatcgaccgt agtctgcaac attgttttat ccagcatctg
     5941 ccgcatttgg tccatcgcga cctcgagggc ctcgatgtgc atggcactga gctgttcacc
     6001 ccaagccagg actctaacca gaccgtaggc tatgatcgcc agcacgccga gggtgaaagc
     6061 agtgccaaag ggcaagcgcg ctttcaaggc cagcaggaag atcaggcaaa caagtccgac
     6121 acccaggatc gcgtccaggg cgggaatcag catcccggaa tggtcccaga gcataagcgc
     6181 ggcgggagcc agaaaaaaag ccgcgattac cctgatgggc acatcaattc cttgcaaagc
     6241 cgcgcggccg ctgaagatca tcattcccac caaactcagg aagggaaagc tgatcgccag
     6301 tgcacccagc agcagaggat aaaacaacac tattcgatta cgtaaggaac gagggccatc
     6361 tgacgggcgc gtttgatctc ggcagtaagc ttgcgctgat gttttgcaca ggtaccagtg
     6421 atgcgggccg ggtcgatctt gccgacgtct gagatgaact gacggatcat gtcgatgttc
     6481 ttataatcaa ttaccagctc tttattggcg cagaagcggc aatatttgcg gcgggcgaat
     6541 ttcttttttc tatctgtgat gttcatttat actcctgatt aaaacggtac gtcgtcttta
     6601 gtagcggtgg gggcgtgttg cggctcctcc ggaaggggaa tatcctgttc ggtgacgcca
     6661 tcttcgcggg gcttccattc cagagtttgg atgtggtccg cgacgatctc atagatcttg
     6721 cggttctgat tgttctggtc cacataggtc cgggcatcca aacgtccttc aaccaggacg
     6781 gcagtgcctt tttgggccat tttgatcaga ttttcagcct gattgttcca tgcaactact
     6841 tccaggtaaa tcgcctggtt ttgccactgg ccggattcgt ctttgaaagc cttgtcgttc
     6901 gccagggtaa accgcagtac cggagtgcct ttgggggtgt acttgagctc aagttcctgc
     6961 gtgattcttc cgctcacgaa gatcttgttc aggcggggta atttgagatc tgccattgta
     7021 tcctcctcat tcacccttgg ccacaaacat atggcgcagg atgttttcgt tgatgttcat
     7081 aaggcgcttc agcactttga ccgtggtgga ttcgaggctg aagtaattca catagtaata
     7141 gccttcctgc atcttgttga tgggataggc cagcattttc cttccccagg gatcggtctt
     7201 gacgacctcg ccaccattct cgcggacgag gttcaggatg gcttcattgg ccgcgtcggc
     7261 ttcgtcgccg ttgagcttag gtgatacgat caccatcagt tcgtaatttt tgatcattaa
     7321 gttcctccta tggacttatg attccgtttc tgtccggggg agtttcagca tcccggaccc
     7381 cagaagcgga ataggatttc atccacttgc tgtactcgtt cagcacagcc gtgaaatcac
     7441 ggttaatata gatatcgatc aactcggttg ctttggaaat tactggcttg atggcaagca
     7501 actcctccgc ggtgagttca tccaggacat aatcggcagc atcgacggat tggtctttgc
     7561 caattcccag acgcagcctt tttagatcat cagcgggtaa aacttcaaac agcgatttca
     7621 tcccgttatg accaccatcg ccacctcctc cgcgaactct taagctcgca aggggcagct
     7681 ccagatcgtc atagacgacc agactttcag agatgtccca acgtctggtt gccgctctgg
     7741 ctgcttcgcc ggaaaggttc atccaggtcg tgggcttgat tagcacggcg tttttgaaac
     7801 tgatgtagtc atagagctta tcgctttgga atgagcgttt atgagctctg ctccattcct
     7861 ccagacacag gtaacccaga ttgtgccgcg tgttttgata gcgcggtccg ggatttccca
     7921 agcccaggat gagtttcatc gctgcttttt acttctcttc agcaggcttg gcggcaggtt
     7981 cggctttggg ttccgccacg gcttcggcct tcttggcgtg gatcactaca agagtcactt
     8041 cctcgtggtc cttgatggtc caggggcctt tgggaaggtc actgacgtgc aaagattcgc
     8101 ccacgcgcag gttgctgatg tccagatcga gtccatcggg tactacatcg gcctgacaca
     8161 caagcttcac agttctatgg acgacatcca caaatccgcc ctcgtgtgtg ccgacggctt
     8221 cgccgttgaa gtggatcggg acttcaaact ccatctgggc ggtagcgggt accaccatga
     8281 agtcgatgtg taggatatgg cgccccacgg gatggaccag cttgtctttc aggatggtgt
     8341 ggtatttgac accagccaac tgcagctcgt aaaacgccag ctcgttgaag ctcttcttgt
     8401 agcaacgggt aaactctgcc tgatcgatcg atatggacaa cgagtccata ttggggccgt
     8461 acaacacggc agggatcata ccctgagcac gcagcttggt gagatcagat tttctcaccg
     8521 ttttacgggt ctgtgcttcg agagtaaata tcatttattc ctccaggttc ttattttatc
     8581 tcttggttga gaagtttgaa tttggggttg agtggtggat gagcagattg gggttatgct
     8641 tgagagggta ttgccagacc aatgagctgc tctaatccgg atcgtctcat catgaaccct
     8701 gctcaagctc aaaactctct tattcattta tctaaacaac acgctcagtg attcttcgat
     8761 gtggatcttc ttgattgcca cagccagcat ttcggcgata gaaagctgca cgattttttg
     8821 gcaattggct ttttcttcca cgagcgggat ggtgttggtc acaaacagct tttcgattgg
     8881 ggaggcctcc aggttttcaa ctgccgatcc agagagcact ccatgggtgc aggcggcaaa
     8941 gatcctctga gtaccttgtt tatgcagagc gtgggccact ttgatcaggc tgccgccggt
     9001 gtcgataata tcgtcaaaga ggatggcggt cttgcccgcc acgtctccaa tgatgttcaa
     9061 gagctcggtc tggtcgttgt tgccgctgcg gcgtttgtct ccaatcgcca ggccgcaatc
     9121 cagtcttttg gccagagctc gtgctctgat agcgccaccg gaatcgggtg aaaccaccac
     9181 cacgttgtcc atcttcatgc tttgaaagta tctggcaaag gacggtatcg agaagaggtg
     9241 atcgaccggg atattaaaaa aaccctggat ctgatcggcg tgcagatcga cggtaatgac
     9301 tctgtcagcc cccgcgacgg taatcagatc agccaccagt ttggcggtaa ttggcaccct
     9361 cggttggtct tttttgtcgg acctggcgta tgcgtagtaa gggatcacgc aattgatgcg
     9421 ctgggccgaa gcgcgtttga gcgcgtcgat aatgatcagc aagtccatca gattatcgtt
     9481 taccggacga caggtaggct ggataacgaa gacatccgcg ccgcggacgt tgtcgttgat
     9541 cttcacaaag gattcatcgt tggaaaactt gaatagatcg atgtccgcca gtggaatacc
     9601 cgcatagcgg gccacttctt ctgccagggg ccggttagcg tttccggtga tcagtttaag
     9661 ctttgcgtac acttttgata ctcttctttg ccaatagtcc tggttttatg cgtccaaaat
     9721 cctttcgctt caaaggcttg gcggcaggtt tggagcgcgc tgttactatc aaagatgcca
     9781 aagcaagtac tgccactgcc gctcatgaca gcggtcctgg ccccggcttc ggtcatcttt
     9841 ctaattatct catcgattaa gggatacctg gatcgaatcc caggttccag ccggttgaaa
     9901 catacaccag atctgaaaaa atcctgataa gacccgcgct gggatactga gctgaggtca
     9961 accagtttat aggcttcggc gctcgatatc ctcaggccgg gattcaccag caggatgttg
    10021 tcaatcagga cgtcatccat gggggtgatc ctttgcccgc gttcttctcc cagacagcat
    10081 ccaccctgca aaaagaagtt gatgtcactg ccaaacctgg ccgcgacctc atcaagcttc
    10141 tcttcgctga gtctcagctc ccagagccgg ttcaaagcta atatggtaat ggcagcgtta
    10201 ctgctgccgc ctcccaaacc agcggcgatc gggatccttt tgaataactc gattcttgca
    10261 cccgcaccgg gacggaaggc ttgccgtaaa tgctccgcca cttgatatat aagattgtct
    10321 ggcgtagaca attccgacac gtcagaccac aattttatgc ccgggttttt tgtcaaagta
    10381 tatttaagcg tatcatacag atcgacactc gcaaacagag tctcgaccct gtggtaacca
    10441 tcgggaagct ttcccaggac ctcaagcgac aggtttattt ttgcgtagga agccgccggc
    10501 atttttcttc tttactttct tgggcgactc ttcaccgtag taataataat agtagtagta
    10561 gtaatagtag taattctgtt tgttgtaata cttttgcacg tagatgctgt tgacgatgat
    10621 gccgtcgatg gtgctgctga tattatccat caattctttg gtgcgcttga tgataccctt
    10681 ttcggtaaat ccgcagcgta ccaccaggaa ggtgaggtcc actttcttgg tgaggatcag
    10741 ggcatcggtt accgcgatca cgggaggggt atcaaagagg ataaagtcat acctgtcttt
    10801 gagttcgtcg atcagcgtat cggtgcgggt ggaggagatc agctcggaag ggttgggagg
    10861 cacaaaaccg ctggtgatca catccagatt ggggatcccg gtggccttgg tgacctgctc
    10921 gatactcact tccggatcga taagataatc ggacaagcca ttctcttttt cgatcccaaa
    10981 cttgttgtgg atcatgggac gcctcatatc catatccacg agcaccactc tggcgttcat
    11041 ttgggccagg gtgatggaga gattgacgat cgtggtggat tttccttctt tgggaccgga
    11101 tgaggtgacc agcagggtag tgctgccagc aggtttgcgg ctcaggatat tggtgcgcag
    11161 ggtgcgatag gattccgcga tgggagactt gggagcgtaa tgtgaaacca gctgcatcat
    11221 cacgaagtgc atggatttgc tgagttgctc cttgttttca ccctcggctt tctcgatcat
    11281 ggcgttgaac tcttccatgc gggcttcgga ttcctgaatg agcggaatcg tgccaacgat
    11341 gggaaggcgc acgaagtttt ccatatcttc cagcgtgcgc agtttcgtgt cgagagagtg
    11401 aactacaaat gccgcgccaa cgcccaaacc gaggcccaga atgatgccca ccaggatatt
    11461 catcgagacc ctgggtttga taggtgccga tggccgctcg gcataatcca cgatgcgaat
    11521 gttgcccact ttggcctgtt cggcgatctt ggcatcctca tatttttcca tcatgatgcc
    11581 atggattttg ccatccagct gcatgttgcg catcaggcgg gcaagctcga gttccgtgtt
    11641 gggcaatgag ataattcgct cgtcatacat ctctttggtc tgggccagac catccacccg
    11701 ggtgcgcgcc atttcaagct ccacattggt ctggatgatc tttacgaggg tctcgttgcg
    11761 gctggccaaa ggatcatcgg tggtggcggc tgccaccagt tttttgatct cgaggtccag
    11821 tttcgttttg gtgcgttcca gttctgtttc cagggccaga atctgtggat ggtccaaagg
    11881 atagttgttt ttggtgacca gcttggtgat cagcgactgg gtctcgatca cctgcttgcg
    11941 aagctcgttg atataggggg tcttaaggac ggaatttaca tttacgacca gggaatcctg
    12001 ttcctggagc tggcgtctgt agaggtccag cgtacgggct ttgacggcct ggtctgtgag
    12061 cgcggcttca tactcggctt cgatctcggc ggaggattcg atcaatttct gcgtctcggt
    12121 tgagagctca gtgagcttgt tcatgttttt gaattcgcgc agatcgtttt cggagctttg
    12181 cagacgacgt gaaatggcat ccagctgggt ctcgaggaat tcacgaatgg tggtaaactc
    12241 cagacgggcg tattgagtgt tttgttgctg aacggcttcc gcgatgctgt ttaccgctgc
    12301 catcgcttca ccctggttgg tggattcaaa actgagggtg aggatatccg tatcacgctt
    12361 ggattctacc ttcacgccag ccagacgtcc ggccggattg gcgctcgcgg cggcaggaaa
    12421 tgattcccac tcgggatatt tcttcatgat ttcccaggcc gcggtgagga tgggtttgct
    12481 cctgatcagt tctatctggt tgttgatagt attcttgccc gctcccggag tggtgagaaa
    12541 gagcaggtca gcactgttgc tttgattttc cagcaaaacc cggttggaag ctgaatagac
    12601 ccttggctgc ctggcagtat atacgattgt aagcgccatc acgatcacga acaccaatac
    12661 gatcagatag cggaattgca ggattatgcg taagtagtcg gaaagcttga tctcgtcttg
    12721 cggttgtggg ggtaagttct gctgttccat atatagagcg ctcctatttc aggttggtca
    12781 cgagattgta cacgctgagg gcgatggcaa ccttggaaag gaagtccgcc accttggtga
    12841 aggcataaaa tatagttccg gaaagcacga ctgtgtctcc cggcatcatc atcggcaaca
    12901 ggttttcgtc ggcggtttcc atgtattttt tgaggttgac ccagataatc tcaggctttt
    12961 catccacgcc cttgggacgg atgatgcgga ttttggtgag ctttgcgtct tctttgggac
    13021 cacctgccaa ggccagtaaa gtgataaaat tggtgtcatc cggcaccaca tagagccccg
    13081 gtttgtagat ttggcccagc acatagacgt tcatcttgag cttttccact ccggtgcggg
    13141 ctccgtcgta tgtgtaagcc gatatccctc cggtgtttgt ggtaggggag gcagtttgcg
    13201 cgaagagagc gcatcccatc gccagtaaca ggattatgaa aatgctttta ttcaccgtga
    13261 tcctcaaaat tcagatttac gttaaaattc cagcatagtc aaatcagtca agtgaaatct
    13321 gtgcctggct gtgctcaaag cggtaagcgg caagtgttat cacatcaatt taccatattc
    13381 aaaaagcttt gcgcattcac aatctgatat tgaaacgccc caatatcaga accatcagat
    13441 agtaggccag cacggtcacc gcgcaagata tcagcagcgc gtagccaaag gccatccttt
    13501 tcatcaatag tggaagtatg gcaaacaggg tgagagacgg caagaccagc cagaagaccg
    13561 aagtggataa ctgtgccact ctggcttgat cacgagtgtc tatccacagc cagatcatgg
    13621 ccagaatcga tacaatggga agggaaacaa ggattccgcc gatcagagtg gagcgtttgg
    13681 aaatctcgct cactcccaca attacagcca agctgataaa ggtcttgatg atgtattgga
    13741 tcatttaggt gcgtcctttt aactgatgca gattttgtgc aaaacagccc aggcgcttga
    13801 attggtcaag aacaatatga attatgagct acctggaaac actgattgta acgcttgtgc
    13861 aaaaaaggac acatggcaga acttggagtt caaaacccct tggcagagtg tgcgtttatc
    13921 aatctgcttg acggggtttt ggacttcaag aagtacatgg gagagatgtg tccacaggtg
    13981 tactggttga agtccaatcg gcccgcaaac tcgttgtcat gcatatcttt atcgtggccg
    14041 attgaactcc aacggggtca tttctatctc tgccaatacc ttgactgcta ctcccacacc
    14101 ggctccaagc accagagcct ataacccggt aaatcaaccc ctacccaccc ccaaccaacc
    14161 tggtattggg taggactagg tctgagtcag caagagggca cttaataggg aagggggagg
    14221 caagttacga aagcacttga cggcattgtg gaatagagaa agattgcttt attggtgacc
    14281 gggatacaag gatttcaatg caaatgccaa tataataaga agataagaag gctgaatgaa
    14341 cataggactt actataaaga acaccggatg tttgagcttt gaatggaagt gttggaaccg
    14401 ctggcctcat cccagaggat tggtcctgtt gcttatatcc atcatcagca taatatccgg
    14461 ttcatgcgct ttcaaccgca tcgatattgt tccaaggtac agactggtga gcaacatcca
    14521 gttcgccatc caggatcatt tggtgctggt gtttgaaagc gcggacaagc aacctgtcat
    14581 tgtgctttat cctgttgata agaagctcag cgcggaattc acggtgcgtc agattcccaa
    14641 aggaggcaag attaagctga atcttgagga gattccggat tctttgcggt ttgagcgctt
    14701 catgtttccc aaaaccagtc gtgtggatcc caacaattat gaagacatgg ttttcgagat
    14761 tggtcaccgg ggcaaagtct atgcgtattt tgacacaaaa accggccagc ttttgggcaa
    14821 atgctattac gccagcaatc tgtttgtgct ggattacctt tcccctctgt tgtttgaaat
    14881 agcgccagca gggaagtatc ccatccagaa atgaccgttt tacggatatc ccggcatccc
    14941 tgcctggtca cccgcggtgg agatgccgtg ccgcccccgg cgaaaaaccc gggagagcgc
    15001 acgggatcac tacggcgggt gaatagggaa gagctggcat taatcaaata gaacgcatat
    15061 gacaggtgta attgagaatt gagaattgaa aattgaggat aagttgtgag ttccgatttg
    15121 attaacctcg ccagtattct gttctacagc catcaaaatg ccctcagata gatcctgtaa
    15181 atcctttttt ctctttttct gcgttccata aattctcaat tctcaattct caattctcaa
    15241 tttctgcgtc cctcagctcc gccttttggg ttgacaagca tcccgccccc ggataattga
    15301 cactcgcata aatttagcat ggagtttcaa tgagcaagaa taaacgcacg gcgatagaac
    15361 cgacccgcga ggaaaactac gcggagtggt atcagcaggt aatccgggcc gccgacctgg
    15421 cagaaaacag cgatgtgaga ggctgtatgg tgatcaagcc ctggggctat gccatctggg
    15481 aaaacatcca gagggtgctg gaccagatgt ttcgcgagac cggccatcgc aacgcctact
    15541 ttccaatctt tatccccaag agctatttcg aaaaagaagc cgaacacgtg gaaggctttg
    15601 ccaaggaatg cgcgatcgtg actcacagcc gtctggaacc ggatggaaac ggcggtttga
    15661 aagtggcggg agaactcacg gagccctaca ttgtgcgtcc caccagtgaa acgatcatcg
    15721 gcgcttcttt ttccaagtgg gtcaattcct atcgcgatct gccgctgttg atcaaccagt
    15781 gggccaacat cgtgcgctgg gaaatgcgca cccggctgtt tttacggacc gcggaatttc
    15841 tgtggcagga aggccacacc gtgcatgaaa ctgaaactga ggccatggat gagacgatga
    15901 agatgctgga tgtatatgcc cgcttcgctt cagattacct ggccattcca gtgatcaaag
    15961 gagaaaagac ggaaggcgag aagtttcccg gagccgtgag cacctattgc atcgaagcca
    16021 tgatgcagga taaaaaagcc ttgcagagcg gtacttccca cttcttgggc cagaattttg
    16081 ccaaagcctc aaacatccgc tatcagaacc gtgccggcga atttgactat gcctggacga
    16141 cttcctgggg tgtttccacc cgtctgatcg gagctctgat catggcccac agcgatgaca
    16201 acggcctggt acttcctccc aagatcgcgc cttcccacat tgccattgtt ccgatttatc
    16261 gcgacgatca ggaaaaagca gcggtgctgg atatggccaa tcagatcgtg gaaatgttcc
    16321 gtggcgccat gctggatgat atcagaatct atacagaact ggacgataga gacatcacct
    16381 ccggtgaacg taactggtat tggatcaaaa aaggcatccc gctccgcatc gagatcggac
    16441 cccgcgacgt tgcttcccag gctgtgatgc tggcccgtcg cgatcaggag cccagagaca
    16501 aaaagtccgt tccgctggta gagctcagag agcatgccat ccaggcgatg ctggatatgc
    16561 aggcctccat ctatcagaac gcggtggctt tccgaaaggc caataccgtc cgcatcgaca
    16621 gccgcgacga gttctacagc tattttaccc ccaaaaatgc cgaacagcct gagattcatg
    16681 gcggtttcgc gctttctcac tggtgtggcg acgtagcctg tgaagagcag atcaaggacg
    16741 atctcaaggt cacggtgcgc tgcataccct ttgacggtga agcggaagac gggaactgcg
    16801 tgtgttgcgg gaaagcctct cataaaaggg tgatctttgc caaagcctat tgatttgaag
    16861 ccggtcagca gtgccgtcat cagcatcgga aacgagattc tgctgggtaa gacggtcaat
    16921 accaacctgg cttatctggc tgcgggactg gccgctttgg gagctccggt gctgtatagc
    16981 gtcacgatca gagacgatgc cgatgagatc accagagcgc tggaatactg cactggcaga
    17041 tatgacatcg tgatcagcac ggggggattg ggtcccaccg cggacgacat caccaaagcc
    17101 acgatcgcgt ctttctttgg caaagagctt gtctttcatg aagatatctg ggagtttgtg
    17161 cagtctctgt ttgccagacg gggtctgaaa gctcctgaga tcaatcgctg tcaggctatg
    17221 gtcccgcagg actttgtcgc gcttgaaaac cagatgggaa cggctcccgg gctgcaatac
    17281 gagtgggacg gcaagtgctt ttttgcactc gccggagttc cagtggaaat gcgacatgtg
    17341 tttgataacc atatcagttc catcattcac gcgcgctatc ctcatctcag caaagtgatt
    17401 cagcgcagca tccacacctt tgggatttcg gaatccgctt tggcagaaag gttggataca
    17461 tcgttaattc ccaaggactt gggatttgcc tggctgcctc agacgggcag ggtggacctg
    17521 agagtctatg ggcacgacga gaatatgata aatgataccg cggacaaact gatagcccaa
    17581 attcctgaac tggtgtgggg cattgatgaa gacactcccg ccggggtgtt gggacgcttg
    17641 ctcagagata aaaacttgac aatatcactg gcggaatcct gttcgggagg cctggtcagt
    17701 aagattttga gcgatgaagc cggagcttcg gattacctgc tgggaggcgt ggttagctat
    17761 cataatcagc taaaacacaa tatattgggc gttagtgaaa cgtcacttgc tgagcatggt
    17821 gccgtgagcg aagaaacagc attagaaatg gtgaccggaa taaaaaggtt gacagattca
    17881 gcttgtgcta tttctgtgac aggtatagcc ggaccttccg gtggaagcga ttccaaacct
    17941 gttggtaccg tgtattttgg gttttcttat cttgctgaag tctggactca gcgccatgta
    18001 tttacgggtt ccagagagat tatcagacat aaggcggctg aaatggcaat cctgactttg
    18061 gcaaaaaaga tcatgggacg cgaccaatga gagtattggt gataggcagc ggtggcagag
    18121 aacacgctat cgcgtctgcc ttcgcgcgct catccaaagt ctcaaatgtg ttagtggccc
    18181 ccggtaatgc tggcatcgct aaagattttg atacccttcc actaagcacg cttcaggagc
    18241 agattgatta ttgcgccaca tatcgtcccg atcttgtctt cgtgggtcct gaacagcctt
    18301 tagaacgagg cctggcggat agacttcgag aattggacat tccgtgtgta ggcccttcac
    18361 aggcagccgc aagaatcgaa accagcaaga ttttcgccaa gcaactcatg caaaaatact
    18421 ctattcccac cgcggctttt tttcacacct tttcactgga cgaagctttg agctactgcg
    18481 ccgttgactg caagtatccg gctgtgatca aagcagatgg actggccgcg gggaagggag
    18541 ttatcatagt gcgggatgcc atcgaagcga ggtccgcgtt agagaccctg atgggcgtcc
    18601 aaaccgatgg gagggcatac aattctcctt ccaaacctca aaaaggagta gtgatcgagg
    18661 aatacttgag cggttgggaa gtatctttat ttgccataac cgattcacag gattatgtaa
    18721 cgaccctgtt ttcccaagat cataaacaac tgctggacca tgacgcgggc cccaataccg
    18781 gtggtatggg tgcctacgct ccggtcccgg aagccgagcc ctacaggtcg gaaatcgaat
    18841 ctcgcattat aagtcccgtt ctggcagccc ttcgcgacga aggctgtcca ttttccggga
    18901 ttttgtattg cggattgatg atcaccgctg agggggccaa agtgatagaa ttcaactgcc
    18961 ggtttggcga tcccgagaca caagcgctgc tccctctgtt ggatacggac ctggtggagg
    19021 tttgccaggc catcgtgact gggcaggtca gagacctgaa actacactgg cgcgatcaat
    19081 cctgcgtggc ggtagtgatg gcttccggag gttatccagg caactttgcc aaaggatatg
    19141 tactgaagat accggccagt ttggagtcca ggctattcta ctccggggtt gagagtaaag
    19201 acgacatgct ggtaagcagt ggaggcagag tgctttgcgt ctcggccacg gggccagata
    19261 aagattctgc ccgcgctcag gcataccggg atattgatca gatccatttc cagaacatgc
    19321 acttccgcaa ggacataggc ttgcgcaaga atgatctata gtttagggtt acgcgtttac
    19381 cttacgcgag acccgcagcc tgcacattgc gtaacgcata acgcgtaacg cgtaaccaag
    19441 aatgaacaag gcggtctaaa tgaagctaca tatatggttg ccgctcctgc tggtcctgcc
    19501 attggcactt tctgccactg gcatcagtgt gagcagcgag acacctgatg agttgattct
    19561 ggagttccag cttccggaat acagcatcga gcacagcaga gacggcagta ccacctggca
    19621 gagaatcgtg tcggattacg gcaacgtgga tgctcaggaa ggttatcctg agctgagagc
    19681 ttactcagag gcgatagccg ttccggtgga tggagacatc agcattcagg tgctgagctc
    19741 aaaaagcagc gtcatcaaaa acatcaatct ctaccccgcc aaaaagatgg tggtgggcga
    19801 ttgggaagtg gactacgagt ttttccagga cccccggata tacaacagca aacagctata
    19861 tcctgaaagc attgcctcca aaagcgaagc ggcttttatc ggtgaccggc gctttattcc
    19921 catccggatc aatccttttc aataccgtcc ggccctcaaa gagttgacgg tgcatagctc
    19981 gatcaggatt cgcgtgagta tcatcggtga taaatcccag gccaaaaact ggcagttctc
    20041 gcagaatccc atcgataatg tggcggatag gttttttctg aacaatgcca gttccaaagc
    20101 ctggagattg gaaaaaacca gagacggcag ttatcagtcc cccaaaaacg gcagcagtca
    20161 ggtttcacgg atccagctga tcgtcgataa agatgggatc tataaagtag acaaggcctt
    20221 tctgcaaaac ctgatcagca ccgagacgga ctctctgggc ctgaccatgg cctgggacct
    20281 cagtgctctt gatccccgca gactggaact cagtgatgag aacgggacgg tccctatcca
    20341 ctttgtgggc gaaaccgatg gggcgttcaa cagtggcgac tactttgagt tttatggccg
    20401 tcgtcatgct ggagatactg gctacatgga cgattacacc gccgaaaacg tttacacgct
    20461 ttatcttaag gacactctgg gtgccaggat ggcggttgaa aacggtggct tgatcgagta
    20521 tagcagcggt aattacatat ctcccgacgc ttatgaatac accgcgatat tccaggagca
    20581 gtctgtaagt gacaaacttg gcagagggtg gagtgccgcc aaccccactt attataaaga
    20641 agacgtgtgg ttttggaaaa agatcagcgc tcccaatctg gaaatcgtgc ccatcgaact
    20701 gcagtttccg atcgacagca ctatccgtac tgccagtgcc aaggttgccc tgcatggcct
    20761 cacctattca gaaaccctga tccctggcca gtgggaccac gaagcatctg tgcgcttgaa
    20821 ccaggcaatg atcaacactc atacctgggt aggccaaaca gagcaagtgt tccaaaacgc
    20881 caaccccata cccaattcct ttatccggaa cggcaccaac aacctcttta tcagcctgtc
    20941 tggtaacacc gtgatggggg atagggaaca ggtcttgctg gactacgtgg aggtcaagta
    21001 ttggcggcaa tacaagacca ccgaagactt tatcaagttt accaaaccct ccaaccgccc
    21061 tggaggcctc tatcagtttg ccctggaagg cttttcgagc ggggacatat cggtttacaa
    21121 aatcggttcc agcgtcttca acagcgtcca gatcgaatct ttcgacctca acggcatggc
    21181 tccctggacc gttactatgc aggacagcgt agcctccacg gctattgaat attacgcatg
    21241 caccgagagc cagaagatga ttcccaagct ggccaggctg gatatcccat ccgatcttcg
    21301 ggatccttcc aatcaagctg agatcatcgt gatcagcagg cgggacctct tgaattccga
    21361 gggcggtacc aggattaaag acctctacga gagcaaggga tacaccgtca tgatggtgga
    21421 tatccaggac gtgtttgacg agttcaacag cggtatccgg gacgcacaat ccattaaaag
    21481 cctgttatcc tacgcttaca acaactgggc ggaaccgcaa ctgcggcatg tgctgttact
    21541 gggtgaagga gtagatgatg agcgtgacaa cagcccttcc agacagtatg cagtaatccc
    21601 ggtcaaaaag acctggacct acaagcatgg cgcaacggct tcagacaact ggtatgcatg
    21661 cattgtcgga aatgatacgg tgccagacat atccatagcc cgagtgggag tgtggaggac
    21721 agaccagatc cctgcggtgg ttacaaaaat ggaaaactac cacaacaatc ctctgcccaa
    21781 tcggctctgg aacagccatc tcaccttcac ttccggaggg aagatcaccg attccaatga
    21841 tatctttgcc cagcagtccg agcggatcaa acgcaggaac gtatcagacg attatcgcgt
    21901 cacccgggtt tatacttcca cgcaaaccgt gagcccggat tactttggcg gcacctttgc
    21961 cctcaaggat gccatcaaca gcggcaccca gtacgtgcag tttatgggcc atggcggagg
    22021 tcgtatctgg gcggattata accttttcaa tttcaacgac gtggccactt tgaacaatca
    22081 gacttatccg atcgtggtga gcctggcttg ttatgcctcg gcttttgata ccaacggttc
    22141 cgcgtctatc agtgaagccc ttatcatgca ggccgacaaa ggctcgattg ccactctggg
    22201 attcagcggc ctgggttatc tggaccagga tgaagattgg ggcctggcct tgacggaagc
    22261 tcttttcaaa gaagactttc cttcggcggg cgaagcggtc caatttgccc tggcaagatt
    22321 cttcaccaca acgacttcca ccgcggcccg ttatgccctc acaaacggag cttcatatat
    22381 gggagatccg acgatcaaac ccagaaagcc ggtaagagac gctcaggttg gcgcggtatc
    22441 ccacgtgctc gctcatggcg atacgctcag agtgaacgcg gtttttccaa acacggtcac
    22501 cgcggcgcgt ctgtatatca tgaaagagaa cgagaaggtg atcaacgtcc ccttcgacct
    22561 tccggtgatc aatggcaact acaatgccag ctatgtggtc cccgccacag gcgaagatcc
    22621 ctatacccgc aaaatctatg tggctggata ttcctctcag aacgagtatg tgggtgtatc
    22681 ggcatttgga gtgggacggc cagccgtttt tcattatgcc atagaacctg cgaccccggc
    22741 ctggagagat tccgtggcct ttatggccag ggctttttcc tcgacgccaa ttctttccat
    22801 gacctgcaaa gtgcgcaccg atagtgtggg aactacgatc aactgggtcg atctgcctat
    22861 gcagcttact gaccctgaca acaatacctg gactaccaca ggtcgcctgg atccccaaat
    22921 aactggaaaa gaagtgtttt ataaatactc gatcagcacc caggacagca ccgggaccgt
    22981 ggtttatgaa tcctttctga gtaatctggt gtacgcgggg cccgaccttt ttctccgaga
    23041 catcaaactg gacaatgagg atggcgagtt cgcgctcaag gtgttggtat ccaatgtggg
    23101 taatgcggaa tcggcagaca ccgatctgcg cttgtatgtg agcatcaatg gcgcaaacca
    23161 gacacagttc agcacccagg tgcttggtcc attggcggta gaagaaatgc gctgggaatc
    23221 cattcccctg gcaggtctgc ccagcggaaa cgtcaatttt gaagtccggg ccaattgggg
    23281 caacagcttc ccggaatggc atctattcta taataccaac aacatcatca ctttgacgct
    23341 gccttataac taccatagtg tcgattccaa tggtctggtc attccctccg tggatgccaa
    23401 tgtcctttgt gaaattcccg cggacctggt tccatccgga agctcagcag tcttttatgt
    23461 gaacagcgtg ggcaccctga ctgccagcaa ccagccagat atccacccca taaaacttct
    23521 ttcttcagac ggggctgcca atccggtatc ctctctggca tatgagatca agaccctgaa
    23581 cgaagcgctc gtggactcca ccggcacctt tgtgggtgga aaaaggatga aactcacctt
    23641 cttctacagc gctctggacc agaccactca gagccaggaa gcagaaaact cctacaagat
    23701 ctatcgttgg gacgcttctg gcagaaaatg gatcctccag ggtggtaata tctccgcgtc
    23761 agaggataga gtcgtctttg aagtcagaag acacggcatc tacactatct tccgcaaccg
    23821 ggacctgatc aggcctacta tcgacgtaaa tgtgcaggac caggagttta ccatcggcgg
    23881 ctatgtttcc gggaaaggca cgatctctct gctgctcagc gatgccaacg ggatcgacgt
    23941 gtttgacaac tcgatcaaac tctacctcaa cggcacccaa gtgcaggata acgagtatgt
    24001 ggcttcgata aatctggata acatcaaccg catcccgatc aagtatcagc tcaatctaca
    24061 aaggggcaac tacaccctgg tggtggattg taaggacgtg aacggaaact tcaacacccg
    24121 cgaaattcag tttattgtca acgaaacctt caacatcacc aagatcggca attatcccaa
    24181 tcccatactg ggaagagcag aagaccccaa aaatgacggt cgcaccagat tcacctatgt
    24241 gctgaccgac gatgccgatc aggttaccat caaagtttac acggtagccg gacgtctggt
    24301 gaaaaccttt accaatctgc ccacgggagt gggttatcat gaatatcccc gcacgctcta
    24361 tgcctgggat tgcaaggatg aagcaggctt cccgctggct aatggcgtgt atttttacaa
    24421 agtgattgcc cgtaaaggca acaagactat tgagaagacc atgaaaatgg ccatcctcaa
    24481 ataggagagc agatgaagca gcaatttatc ataactgagc aggatgagag agctgagatg
    24541 actggttgtc acagccctcg aacccttcca cccttccatg cccccagact ccttctgata
    24601 ttcctggcct gtgtgtccat tctctcttta agcgctggac gctatgcggg agactttatg
    24661 atgatcgggg ccggagtccg gccgcttgga atgggaggag cgttttcagc cttggctgat
    24721 gacggttccg cgatcttttg gaatgcttcc ggtattgcag gcatcacaga aagggaagtg
    24781 gcggccatgc acgcttttct gtatggaggg ctggcatcct atgacaatct cagttatatc
    24841 cagccactgc caaacggggt gagcattggt ctcaatctca ccagactcac ggtaagcgat
    24901 atcccgtatt tttcggaaag ctatctgatc gggacgaatg tggaccaacg tatcaacaac
    24961 agcgcctacc acttgcctgg agtcccggac agcgagtttg acagtaccga tgatctttat
    25021 cagtttgcct ttgccaagca ttttcgctat gacgccaata tgggctggtt gttctttgaa
    25081 attccctttg atttctacct gggcggtaac gtcaagttta tcaaacggaa actgaggaca
    25141 aactacggaa ccgggactgg tgtggacctg tcgtttaaga tcactacaga tatgggcgtg
    25201 atcttcgatg tggaggaatt ggggaagctg gattttgcca tgaatttcca ggatgtagcc
    25261 ggaaccgata tcgcgtggga cacggtgaac aagatcaagg acgaagtgct tttcaacacc
    25321 aaactgggcc tcgccatccg tcaacccata ccccctctga aatcagaact caaactggcc
    25381 tacgattatg actatgtata tgaaggcacc agccactatg gcgtggagtg ggactataac
    25441 aacaaaggaa gcctacgggc gggatattat gacaagaata tcagcctggg tgcttcagta
    25501 aagatctacg acgtaaatct cgattacgcg ctggttacca atccggtcgg tgtcacaaac
    25561 cgtctgggtt tgcgcttgag attctgaccc gggagaatac catgtccaaa accctgcaac
    25621 ttattgccct gctcatgtta accgtgattt taatatcatg ctccggcact gataaaccgg
    25681 aagaagacac cactccacct tttccaccca ccatggttcc ccacctggga gacgtgggag
    25741 atccacccac cacctattat ggacaatcgg cgattctgac ggaagacaat aacggcatcg
    25801 acacggttgc cgacggaaac tggatcagga ttgcctggaa accctttata gatgaagatt
    25861 tgaacctggt acggatctgg cgctttgacg aattcaatcc cactccggtg cagattgatt
    25921 ccatcgctcc caacaatgac tactatctgg acagccggga tcaactaccc gagagaatct
    25981 ggtattcata tttcattgac atagtagact ttgccggcaa tacagcccgg tctgacactg
    26041 tatcctatgc cctcctggct aaaccggtgc ttctggaccc tcccaatggg gccaccattt
    26101 ctccggtagg ggccagagtg tattggaaca gcagcggttt tgcctctatt tacaggctgg
    26161 ttttgtttga ttccaatcat aactatttgt ggcatcagga cctccccact gcgatggaag
    26221 aacagctctt tatggatatt ccggtaaatc tactcactca gcactccggc gagggtattt
    26281 actggcgcat tgattccttt gactgggatg aagaacgtca gcagtatctg ggtgccgaat
    26341 ctttggaacg gatcgtctat atccagtgat ttagacttct aagtgaagcc ctgccatctt
    26401 ggcagggctt cacttttata ttaagtaagg gatttcggca gaaatagact attcttgcga
    26461 ctttctaagc gtctgtgggc tagatgatta ggacattaat tcaggatttg gaagcagaac
    26521 ggcatcagct tttttgggct taggtatcaa tcttgcttat cttataagga caatgaattg
    26581 agcttggaat ttttgttaac caaatataac taagaagaat gaaaggagag gagatgaaga
    26641 agcacttcat actactcatg ctactgagcc ttgcggcctt attaatggca gcgcagggaa
    26701 gcatcgtgtt ttctcagaat cccagttctg tgcagcttct gcgtgaagcg cagaatgggc
    26761 tgagcgtacg ctataccctg gacaaattgg attaccagga tatggacacc cctgaaggaa
    26821 gatttacaga cctgtctgtt cagcattata ccagcaccaa tgtggccggt ctgccaaagc
    26881 tgccattgca gaggcagatt attgccgtcc cggaaggtgc caccgtggtg cccagcatcg
    26941 taaccgcaca aagacagatt attgcgttgg cttcccgcgg gatcaattat ccgatcatgc
    27001 cacgccagga atccgtctcc aagagcgcta atcctgccga catttctttt gaggtcaacc
    27061 gcgatttcta caaccgctca gcctggactc aggaaccctt cgtccgggtg gaagagatcg
    27121 gttatatgcg cggtgtcaga ctttttgccc tggattttat gcctgtgcgt tacaatccag
    27181 ctcagggcga gatcgaagtg atcgtcgaag ctgagatcag agtggacttc gccggggccg
    27241 atctggctgc caccgaagct ctcagagaac gttattactc gcccgctttt aatggcattt
    27301 accagaaaag cctgcttaac ccattagaca acagggctac tctgaaccgc tatcccatga
    27361 gctatctgat catcacacct cccaactttg tgactgccct gcagcccttt gtggattgga
    27421 agatcaagga aggcttcaac gtgatcgtgg ctaccacaac tcagacgggt accaccgcaa
    27481 acgcgatcaa aacttatctt cagaatatct ggaacaacgc cacaaccgcc aaccccgcac
    27541 ccagttatct gctcatcgtt ggtgatgtcg ctcaggtgcc atcaaataca ggcacaaccg
    27601 gttcccacgt gacggacctc acgtatgtcc gccttcaggg caccgacttt gtgccagaaa
    27661 tgtactttgg tcgtttttcc gccaccaccg aagcccaggt gaccaaccag gtcaataaga
    27721 ctctcatgca tgaaatgtac accatgccca gcgatgccta tcttggcgac gtggtcatga
    27781 tcgctggtgc cgatcccagt tgggccccca cgcatgccaa tggccagatc aactacggtg
    27841 tcaacaacta tttcaatccc gctcatggga tcaatagctt cacctacatg tatccggctt
    27901 caggatcctc cgacagccag atcatccaga acgtctccaa cggagtgggc tatgtaaact
    27961 acactgccca cggcagtgag accagctggg ccgatcccac tttcacagta gctaacgtca
    28021 acgggcttca aaacgtcaac gaatgcccgg ttgtggttgg caactgctgt ctcaccagcg
    28081 ctttcaacac caacgtctgc ttcgcggaag cctggctgag agccgaaaac aagggcggag
    28141 tgatctatat cggcggcacc aacagcacct attgggacga agattactgg tggggagtag
    28201 gttacaagcc ccccgtggta ggttccggct ctcctttcat cgctggccgg accggtgcct
    28261 acgactcgat gttccacgaa cataacgagc ccctggccga ttgggctacg agcgtcggcg
    28321 ctgccacttt catgggcaat atggctgtgg tgcagtcaaa cagctcccgc atcaactatt
    28381 attgggagat ctattccgtg atgggcgatg ccagcctgat cccatatctt gggattcccg
    28441 ctttgaattc tgctgtagtt cccgagacga tcttcctcgg tatgggcagt gctgacatta
    28501 tcgctgaccc ctacagttat gtggccattt cgatgaatgg cattttgcat ggtaccggtt
    28561 tggtggatgc cactggcaat atgaccctga gcttcactcc tttcacggag ccgggaacag
    28621 cccagctggt gatcacgcgc tccatgcgta aacccctgat cgccaacatc caggtgattc
    28681 ccaattccgg gccctatgtg accgtcagtc ccatcaccgt caacgatcct aaccagaatg
    28741 gcctggccga agccggtgag atcatatcca tggacatcag ctttaacaat gtaggagtag
    28801 tggaagccac tggcctcact gccagcctca ctaccaccaa tccctgggta tcactattaa
    28861 cggccagttc cccgattcca aacgtgccag caggcagcag catcgatgtc acagacctgt
    28921 tcagtgttca aatcagtccc atgattcctg atcagcaggt catcaatttc gacataagca
    28981 tcacagacgg cagcaatacc tggtcctcaa gccgcagtat cactgcctac gctgccgatc
    29041 tgcaaatcac cggcgtgacc atgaacgacg gcaacaccaa tggcttcctc gagcctggtg
    29101 aagtagtcac catccagatg gccttagcca acaacggcca catgcccatc agcggtggaa
    29161 gcatggtgat cgtgcccaac catcctggcg taactctgga caacgaaagc tttgtcctgc
    29221 ctcctatgtc gattggggcc agcgctcaaa tcagcgtgat cgcgtttttg gctggcgact
    29281 tgcagacagg aacggtgatt tctctgggag ttgctatcaa tgccggcgcg cagatgatca
    29341 accacaccat ttctctgccg attggtatga ctggcgagga ttttgaagcc ggaaacttca
    29401 ccaactatcc ctggaccaac aacagccccg ttccctggac catccagagc ggtgcaaaca
    29461 acacccatgg tggcacttac ggcgccaaat ccggcaccat atcgcacaat ggtaccacag
    29521 agctgtcttt gaccctcaac gttggcgcgg ccgggaacat cagcttctgg agaaaagtct
    29581 ccagcgagag cggatacgat ttcctgcgct ttctgatgaa tggcactgaa atggcctcat
    29641 ggagcggaac tcaggattgg acccagatga gctatccggt ccagcctggt ccgaatacct
    29701 tcagctggaa atacaccaag gacggatctg tgagttccgg aagcgattgc ggctgggtgg
    29761 acgatatcat cttcccgatg gctggcagtt ccaacgcggc cattttctat agtcccaatg
    29821 agaccatctc tttcacggaa gttgccgctg gcagcactgt gagtgccgac ctggtgattc
    29881 gcaatctggg caatattact atgagtggca tgatcactat gcccactggt ttcgtgctct
    29941 cgatgaacgg caccgtcctg cctcccgatt attcctacaa ccttgaggcc gggctcaatc
    30001 aggtgtttat ggtcagctac actgctccca ctccagccat caacctcgac gatgtgatca
    30061 tcgtttcatc caatgatgcc aacaatcccg ctgtcatgat cccaatcaca gtaagcgcgg
    30121 tggtagcaaa cgatgatcct gtgattccag ccgtcaccaa gctggaaggc aactatccca
    30181 accccttcaa tccagagact gccatccgct tctcgacccg tgaagctggt cccgtgaggc
    30241 tcagtatcta caacctgaaa ggtcagctcg tgaaatccct gctcaattca aacctgccag
    30301 ccggcaatca cagagtgatc tggaacggca aagacgagag cgggcgcagc gtctccagcg
    30361 gtatttatat gtatcgcatg gaagcggccg gttactcaaa aaccctaaaa atgatgttga
    30421 tgaaataaat caatctgtcc aggcggcatc aattggtgcc gcctggatgt tttggaaaat
    30481 gagttgatca tgaggcccgt tttttttggt tgacagattt tgcggtccac ataatctact
    30541 cgttttagca gagattaaca ctgaagcgga gaatgccggt ctcagacttt cgtcggatcg
    30601 gaatcctacg ctcctcatac attaaataaa agtcgtgaat ccatggagga tccaatgaaa
    30661 catttgatac tgaccctgac cctgctgctc acccttttgg ggctgacagc ggagcaggtg
    30721 accgtcgggc ccaatcaaaa ccaggttcgg cttgtacaaa gccagtccga caacatggtc
    30781 ctggagctca ctctgggcca tttcaaccgg gaagccgttc aaatcaacgg cagcacttgg
    30841 tatcatatcg atcttaaaaa agctggcctg actctggaga gcggtttgcc tcagcttcct
    30901 gtgatggctg gcagtgtgat catacccaac acggccagga tggagctcag caccgtcctg
    30961 agtgagtatg tggatattcc catgcaggtg gcaccctcca agggcaattt gacccgtgat
    31021 atcgatccgg acagcgtgcc ctacagcttt tctcccttct actccgggac cgaagcatgg
    31081 ccgcagagca actccgaatt gactgagcca tttatcctca gggactatcg tggcatcacg
    31141 gtccgcttcg tgcccttcag ctatttcccc tccaccggaa ctttgagagt atacaccaaa
    31201 atccaggtag cgctcaacaa caccggcacg gacctcacca attccctttc cgttcccaag
    31261 aacagctata gcgaggagtt tgagggcatc tacgaaaaca tgttcctcaa cttccagcag
    31321 gccaagtatc cctctctggc agaacaggga cgcattctgg tgatcaagca caacatgttt
    31381 gacactgata tcatgccctg ggtgaactgg aagcgtcaaa acggctatca ggtggacgtg
    31441 gtggatgtca gcgtggctgg ccctaccgcc aacaatatca aaacctatat tcaaggccag
    31501 tatgacctca acaatggcct gatgtttgtc cagatcgtgg gcgacgctcc tcaggtgcct
    31561 accctcactt cgggtggagg aggctctgat ccttccttcg cgcttctggc aggaggcgac
    31621 agctatcccg atatctatgt gggccgcttc tcagcccaga ccgtcacgga gcttcagacc
    31681 caggtcacca aagccgtgga atacgaaaga gatcgcgtag ccggcaatgt ctggctccaa
    31741 aaggctatgg gcatcgcttc caatgaaggc ggcggctcac aaggcgatat gggagaaagc
    31801 gatcaggccc acatggaact gattcgcacg gacttgcttg gttatggcta tacttccgtg
    31861 gaccaaatgt atcagaccca gggagccaca gccgcacagg tgagcgccaa tctcaaccag
    31921 ggacggggct ttatcaacta tgtggggcac ggatctgaca cctcctgggt gaccacaggt
    31981 ttcagcaaca cccacgtcaa cgctctcacc aacgacaaca tgctgcccgt catcgtctcg
    32041 gtagcctgcg taaatggcaa cttcaccagc cagacctgtt ttgccgaagc gtggctgaga
    32101 gcaaaaaaca acagcaccgg cgagcccact ggcgctgtag caatgtatgc cagcaccgtc
    32161 aaccagggtt ggaatcctcc catgcgcgga caggatgaag tcaccgacct cctggtcgcg
    32221 gaagccaaaa agaccatcgg cggcctctat ttcaacggct cctccaaaat gatagaagta
    32281 tatggcacca gtggcatcag tgagtataaa aactggacca tctttggaga cgcgtctcta
    32341 atggtgcgca ccaaagaccc catgcccttg actgccaact acaatcccat tctctttatg
    32401 ggaatgaaca gcttcaccgt ggatactatc gccggggcca gagtgaccct ttcagccaat
    32461 ggtaccgtct atggccacgc catagccgat ctgactggaa acgcggtggt ttcgattccc
    32521 acccctccag tggagccgat ggacctcacc ctcaccatta cagctttcaa ccgtgaagcc
    32581 catatcgcca ccgttcaggt gcttccctcc actggacctt atgtggtggt taccggaatg
    32641 accgtgacgg acaacaacaa caaccaggct gactttggcg aaaccgtcaa catcggcttt
    32701 ggcctcaaca acgtgggctc cggtgccgct cagggagtgg tgatgaatct cagcaccacc
    32761 agcccttatt tgaccgtcct gagtggacag gacagcatcg gtgacatccc ggctgaaact
    32821 accggcggta ctgtcaccgg cttcacaatt caaatcgctg ccaacgcacc cgatcagcat
    32881 gtggcccctc tggacctgtt gatcaccctg agcgacaaca ccacctacgc ctacacccgc
    32941 aacctcactg tcaacgctcc caacatgcaa tggggtgccc ttttggtgga tgacagcgtg
    33001 ggcaacaaca atggccgggt cgaagctggc gaaaccgtca ctctgagctt tccgatcaac
    33061 aacctcggtg gtgctcaggc caacgatctg atcagcgctt tggcgatcaa caacgggacc
    33121 aacgtctcag accctgtgca gaccaccttc acagcccttc cggccggtgg cagtgtcaat
    33181 atgatttaca ccgtcacctt cagttcacag ataccggttg gtaccaccat caatatgact
    33241 gccatggtat tcagcggaat gtacagcgcc atgcagacct acacgatgaa cgtgggccag
    33301 gtgatggaag gcttcgaagg aggttttggt ggctatgact gggtgtttgc caacggcaac
    33361 tggactaccg ttccgggaag ctacaacggc accacggctg ccaggtcggc cacgatctca
    33421 cacaaccaga atacttcgat gtctgtgacc atggtcagtc aggctgccgg aaacatctca
    33481 ttctggaaga aggtttcttc cgagcagaac tatgactacc tgaaattcta tatcaacaac
    33541 accctcaaaa accagtggag cggcaccaat gacgtctgga gccagctcac ctatcccgtc
    33601 aacgctggca ccaacaccta caagtgggag tatgtcaaag acagcatggt ctccagtggt
    33661 gacgattgcg cctggatcga cgacatcatc ttcccctcca caggtgggac caccggagct
    33721 cccatgatat ccatggatat caccagcctg gattacggtg acatcctcct gggtgagact
    33781 cctgtcctgc ccgtcacgat caacaacgtg ggtgacgcgg tgttaatcgg cacggtggaa
    33841 acatccgctc ccttcagcat tgtcgatggt ggaggcaacc tactcgaaca gatgaatgtg
    33901 gtgatccagc cttccagctt cctccagttc gatctgcggt tcgctcccag cgcggctggc
    33961 agcttcgatg gcagtctgat tatcaataca gatgattctg ccaatcctag catcgtgatc
    34021 accctcagcg gcaccgccaa tccagtcgcg aacgacgatc ccatcctgcc agctgtaacc
    34081 gctctcaagg gcaactatcc caatcccttc aatccccgca ccacgatcag ctacagcgtc
    34141 aaagagcaag gtcccgtttc catcggcatc ttcaacctca aaggccagct cgtcaagacc
    34201 ctggtcagcg aagccaaagc tgccggagac cacactgtgg tctgggaagg cgatgatgat
    34261 aatggacgtc aggtctccag cggaatctat ctctataaga tgtattccgg caaatacacc
    34321 agcaccaaga aaatgatcat gatgaagtaa ttcattaggg atcaaaagaa tacaatgaag
    34381 cggagcggga gactgctccg cttctctatt atgccgacga acagaacttc atggaaaaac
    34441 ctgtgcatac cgccgttgca atggctaata aacttatgcg cgactattgg ttacttattc
    34501 ataagagccg tcatgcgaac aggcatctcc cacccaagcc caacaaattg gggatgggtc
    34561 cgatgtgatc agggcaagga tgtgctcaac ggttatggtt gagttaaaga ccataaccgt
    34621 gccgggaagc tgagtttcag aaacacacct cccattcgtt cccagttacc cgccgtagag
    34681 atgccgtgcg ctctccggcg tttttcgggg gaggaggcac ggtatctcta cggcgggtaa
    34741 ccagcaagga acctgaggaa ctcgtcgaga ccattgaata atcggtatca ctggcttcag
    34801 agctagtgaa aaacatcacc ttgaccgtct tggagttcga agccccaaac aaattgtacg
    34861 tttatctcta tacctgatgg ggcgttggac ttacgtgcag tacttcgaag acatatatct
    34921 cgcgggtgta ctggttgaag tccaatcggc ccgcaaactc gctgccacgc
//
LOCUS       DAOSFN010000007        34821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_975_length_34821_cov_8.998130, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000007 DAOSFN010000000
VERSION     DAOSFN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 34821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34821
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_34821_cov_8.998130"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(385..1152)
                     /locus_tag="PLO35_01150"
     CDS             complement(385..1152)
                     /locus_tag="PLO35_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH78297.1"
                     /translation="MTPSVAVAPSGGSGTIFFSHCNLRCVFCQNYEISHLGWGQAYQA
                     DEVLKMMLDLQSQGALNINLVSPTHYTPQLIPVLRQAKVSGLRIPVVWNSNAYEKVEL
                     IQALEGLVDIWLPDFKYAYGIYAAKYSGVRNYPEIAMNAIAEMYRQGGDLATDRHGIA
                     KRGVIIRHLVLPNQLSGSEAALRKIASRIGTSVTLSIMAQYYPKGKAAEYPELMRPIK
                     AEDYARVLETASELGFEHVFAQELIPTPDWTPEFREL"
     gene            complement(1329..1715)
                     /locus_tag="PLO35_01155"
     CDS             complement(1329..1715)
                     /locus_tag="PLO35_01155"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOH78298.1"
                     /translation="MEKDVLLSLLDDAKKAAQAAYVPYSGYMVGAALLGVSGKVYLGC
                     NVENSSYSLTICAERTAVFKAVSEGERDYLAIAVWVESDKPFPPCGACRQVLHEFNPG
                     LSVIYGNKHGYVITDMAALLPGAFKL"
     gene            complement(1727..2827)
                     /locus_tag="PLO35_01160"
     CDS             complement(1727..2827)
                     /locus_tag="PLO35_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOH78299.1"
                     /translation="MEAITRGLTHLYWDQAVMMIVGAVLIYLAIAKKYEPALLLPIGF
                     GTILANIPFSAAVVDHGALTILFNAGIRTELFPLLIFIAIGAMIDFTPLLKNPSMLLF
                     GAAAQLGIFVTLAIAALMGFSLKEAGAIGIIGAADGPTSIYVANRFAPSLLGPISVAA
                     YSYMALVPIIQPPIIRLLTTKQERRIHMDYHSGDVPRIVKIVFPISITLIAGLVVPDS
                     VSLIGFLMFGNLIRESGVLESLSKSAQNELGNIITILLGITIASTMKGEDFLKPETLF
                     IMMLGLVAFVFDTAGGVLFAKLFNLFRKTKINPMIGACGISAFPMSARVIHQMGQKED
                     PFNFLLMPAVSVNVGGQIGSVIAGGLILALLA"
     gene            complement(3038..5011)
                     /locus_tag="PLO35_01165"
     CDS             complement(3038..5011)
                     /locus_tag="PLO35_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH78300.1"
                     /translation="MNRFYLFIVFLAVSALAALSIQPVDPDRAMSTSTHNSGSIGLTV
                     SNYGFSTNLLYPSTRQHKLMYASAPWIGAKRYRRDPSGHLYYWLSPNPDQFNAGMVTQ
                     FDPGWNSSLKPVVDTLCTVGFDGDQDLYELLPAYNPLAWNSPQYSLFNSADVALKSIL
                     GSPAPRPFVIPDPQQTYCFSLPQGGTFVTPGFETVSAWFYDYSPLYAYANGDLGASHN
                     MNTHYPLGLAIHRESYAWNLQNYDRAVIYKYTMINSSPIDTLFDLAIGEYVDADVHPA
                     GQGVEGAADDASGYVRGAGLDYAYSRDFDHDGGLSPYILANKLIIPGFNGNRNAWFWK
                     VGDGPDDRDPRGFNYIPNLTSNEKYWLLTGRNANYTKYVSLFPQSPDGQYEQPAANDT
                     RFLHCLYGSQPEAAQPNPPGRVHLAPGQSFSWYSVHFVGSQISDLNLLAASLETFITQ
                     QNLQINPSAGLTCIPYLMPVQIVNEGGVELNWHSYTDPDHFEVMYKLLDAPSSDWVRT
                     NKAGSERWHAISGLDSDGWYQFRIASIYFVGADEVYLESETQIIRPGAVAIDDPQIPV
                     PQLALKTWPNPLRDRCDIGFELPKAASTKLRVYNMKGQLIRTLISANLASGAHQSLWN
                     GLDESGHPCAAGVYLLKLESGNFQGAKKLLLNP"
     gene            complement(5008..7167)
                     /locus_tag="PLO35_01170"
     CDS             complement(5008..7167)
                     /locus_tag="PLO35_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78301.1"
                     /translation="MKTYPVILWLPLLLALMTSCQEPLPLTPTPAQPADIICSEDKLP
                     LGGDEYIYRQSLCAGTDSHQGSLYAWKLHTLSGEPPSGSQLDPDGWLLREDGQSIWTS
                     EKELNLDYRSQNGKLTDLITSATLRIKAPNGQITEFHSPFKTQRIVSSHIFVTFPNGA
                     TTSTGLCFNLAESVGDIFVEGLIADHFMYRLNIINSSLDVISHGEWYSSITSPDIRQV
                     ILNESTLPALEPTPNTRYTQFEAYVVSRQGVEEATPKSVYFHADGDFYPDAVIHSNLI
                     AGLGDHHYSINRNEQIYDMELIPSQTYRRNGILWKTGNVYTAVHSPNFKLHLHWGYNG
                     QYGYQFNGNAQVTNNPLDSHLDNCVSESGVDYHGHIVAYDLSLDGERFPVLPQFIAPQ
                     FVTHADGSMWLRVRNIGDSASACVLSGLSAAPHIFKVCAVDAQGRYDQTPAVITINLV
                     PYKAPPLRNGILIVDGTYNNSTYTPEALVDAFYASVVPTTWGPVETHEISYNDPWNAA
                     LNPALLQNYKAVIWHQENPSSSGDINLYGDALEVFLDGGGKLILSGNHWLNSKLNYFM
                     DHYPELVTQRFGISNLNCFSLLSTSVISNPWFIGAEGLDGIADIDLELEDPMVGVVET
                     RQGIGGVLCYDGSQQAGELHRFVCKPVTSTVSPPNQDQYDTFSSKYVSFAHQQDMGIT
                     AVFGFPLSYMEVADVSQALSDLLSAWLGTSYASGGHK"
     gene            7464..7733
                     /locus_tag="PLO35_01175"
     CDS             7464..7733
                     /locus_tag="PLO35_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOH78302.1"
                     /translation="MAVWELTVTGRVQGVGFRWYAKHLAVELGVKGYVRNLPDGSVRI
                     LAQADQLTLERFGTEVRMGNRHALVAEVHIMTLDHASEYYDFEIR"
     gene            7813..9054
                     /locus_tag="PLO35_01180"
     CDS             7813..9054
                     /locus_tag="PLO35_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH78303.1"
                     /translation="MLLFLLPVLLFADFEYHSVKQGDTLYGLGKRYGVSVAQIKQLNN
                     LSSDNLSIGQKLKIKEKPKPQAKPKPKPVATAEPKPAVPAPVTITAPPPPKPAAPVAV
                     ATPQPENPGPSPASGQLAVPAQELPKEFYHIVQPGENPYRISQNYGISTQNYFLWNGK
                     QSWDEFDIHPGDKIIIKDPALAKPGSRSEVPPVQQPEAVASPSPATPTVSAARDTVVI
                     TKTYTVKPKDTLFSISRANNMTVDEIKRINNLSSNAINVGQVLYLAGTPPSGAPRQST
                     PPRTEAELEAKDVLRTDLFTPVQGKVVSEFGIRNGKPHKGIDISAKTGTPIYSALDGV
                     VVFSGVQGNYGNVIVVEHPDFVMTVYAHNDQNLVSVNDTVKRGQMIAYIGSTGNATGP
                     HLHFEYRIKGKAINPRKVLSL"
     gene            9057..9950
                     /locus_tag="PLO35_01185"
     CDS             9057..9950
                     /locus_tag="PLO35_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOH78304.1"
                     /translation="MPTGMSSSKRESVFADKALQNYLREISKYKTLSREEEHQLGIRA
                     KNGDTEALNRLIQSNLKFVVKIAARYQNRGLSLSELISEGNIGLIKAIEKFDPDKDIK
                     LISYAIWWIKQRIMLAVSEKSSLIRVPLGKTSTAHRIKAVQDRIFSETGESASSQVLG
                     DATNMSAKSVDQLKDQIVETTSFDEIIQSSDYSEYSTRDLLEDKDTIDPQQLYHRERM
                     QERLHNAIDKLDNREADIIRDYFGLNENNEAQNFAQIADRIGLSRERVRQIQKEALRK
                     MLAELKPDEDAFVDEFIDRYG"
     gene            9980..10882
                     /locus_tag="PLO35_01190"
     CDS             9980..10882
                     /locus_tag="PLO35_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOH78305.1"
                     /translation="MFRNFDEMKDYVKQGRRGRVVIAAAQTDSVLDAAILAAQEDLAD
                     CILVGDAGAIRKLLETMAPDKVSAFEIIDTGADLVKAARTSVALVKEGRGDIILKGKT
                     DTSVLLKAVLDKENGLRVSEVISDVLAYEHPDGIRMMSDGGINIAPELNEKIAIVRNS
                     VQVAHAMGIPNPKVALLSAVETVNPKMPSTLDAAIISRMNKRGQIAGCSIEGPLAFDN
                     AVDIEAARIKGIVSDVAGQADILIVPNIEAGNIFGKTLTYYCHYRVAHVVMGTRAPIL
                     IPSRADDGETKMLCMVMGLASMTR"
     gene            10942..11406
                     /locus_tag="PLO35_01195"
     CDS             10942..11406
                     /locus_tag="PLO35_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOH78306.1"
                     /translation="MNLALVQIGKTKDRWLQEGIDEYLKRLGPYHNLNIIELPDHSRL
                     NSQNSELVQSREADAALKQIGEDDYLVLLDEAGQLKSSLEFSRFLTSLSDKRRVVFLI
                     GGAFGTHKRLKDRANQILSLSPLTFTHRMARLILIEQIYRATMIAQGRSYHI"
     gene            11419..11574
                     /locus_tag="PLO35_01200"
     CDS             11419..11574
                     /locus_tag="PLO35_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HOH78307.1"
                     /translation="MITLITNPTMSNFGQALLILLQGMTGIFFFMALFYLLILGLEKL
                     FKPRTSQ"
     gene            11571..15416
                     /gene="porU"
                     /locus_tag="PLO35_01205"
     CDS             11571..15416
                     /gene="porU"
                     /locus_tag="PLO35_01205"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOH78308.1"
                     /translation="MKRALTTLLALLMVLSLPAGVTLLQTTGRDLTLEYRLDPYKLIE
                     ENGFITISMPGMDYPSLSGSPLLPYLESKIGLPPGGQMAWDLQSVEEEHITLKQRLTP
                     APTMKFVNDMSRSDYLVDEALYAANPDYTTALEAQNWRGFRFAALRITPFRYDGQTRL
                     TILKRAVFSIRIQGDLEYREQAGIDELMELMLGQFVNGEQAKAWQSFSRNPINYAPFA
                     SADQWLKIETDKEGMYKLSYQQLSALPLADIDPRTFRLFSTGGELRPFQVVTPGPEFQ
                     ELAIRVVGEEDGHFDSGDYILFYGSSRDGTLKNQSLDVVPTSFNPYSQNSVYWLSFGG
                     SFSGPPRRMDTLPQPGSYTKAVHTQLAQSRYETETHRRTQIGFIWYSSRLFGNSTAEY
                     QYTLPLSNLNARGDSLLTIRFRQEEIDSDIWHYINVFVNDIEVAPPSGQTSFSWQGTG
                     EYIFQKYVTSFVNGDNVIRVRVNRNGTDNLFLDFIAVDYPQTLIKGVGQYLITPDPTL
                     ISQTVRYTLPGGIGEAEVFRALDYADVSKVPVVSEPDSLWFAASGTNASRFIISTPSE
                     YYSPVSITEVNPVDLTVSPSQVDHVIIAPAEYLTQAQTLADMYADFHGLSVKVVDQQD
                     IITQFNGGHPDPAAIRQYLRYVYHHHPAPRLRGVTLIGLGTFDWRNQSRQSSAKNKLM
                     IYQRGESSSDDFYAMMSSSFYPELIIGRYPVRNTNELNNMLSNYREYNQNPQGGWWRN
                     SMVMLGDDLYNGSLDHYENIHTRQVELAGNVVHPSIQVDKIFAWEYDYDEYQNKPQAR
                     DDMLAAVNEGRLVWYYIGHGSFDSLGSEDFFNGATDMGRFNNANHLPLFMAASCKVSQ
                     FDHWGFESLGQKVVMLNNLGAIASFSATRLSSPYSNAPMGEKILDNLANKRNYLGYSI
                     MAAKLASQTDDNDHVYVLLGDPTLRIIPPQRDSLMSVSTGIARDGKTTLQSRQKVDVQ
                     GSLNPYSGAGTAEIKVFDSAVFYNLDAQSRVSHRGTTLFKGSATVSSGQYEAAFIVPD
                     DITNGDTGSIVSYLWDAQAQRDYVNYHYPVTTSDQAVQAENLSAPAIELFLGSYDFRP
                     GDTVSTSPTLLARISDDNGINLTGSAGHNILLVIDNSIQPIPVTSYFSYDQDSYTSGI
                     LRYPISGLSEGPHSVQVVAFDNFNLPSVTSTQFIAKKTGALSIERLLIYPNPIENEGH
                     ITFILSEDSDLDIGIYTITGKRLRRIQTPGRQGFNQIPWDGRDHNGNRLANNSYFVKV
                     TAKSGSRTAEARERLVIYK"
     gene            15425..16813
                     /gene="cysS"
                     /locus_tag="PLO35_01210"
     CDS             15425..16813
                     /gene="cysS"
                     /locus_tag="PLO35_01210"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOH78309.1"
                     /translation="MQIYNTATHRKQEFIPVKPGKVSLYACGPTVYDYFHIGNARAFL
                     FFDVARRYLEYRGYDVTYVQNITDIDDKIIARSIEAGIPFSEFAAKYASAFLEDSAAL
                     GIKPPHHQPRATQMMPQIIALIEQLVQHGYAYEVEGDVYFDTARLPEYGQLSGKKIED
                     QLAGARVAENTLKRNPTDFTLWKHSKPGEPIWQSPWGEGRPGWHSECVVMGQHYLGQT
                     FDIHGGGVDLVFPHHENELAQAYALSGQPLANYWVHNGFLNIEGEKMSKSLDNFFTAR
                     DILAQHSAEAIRFFFLSKHYRSPIDFNREIIAESERAINNFRESLKAIDYLKLPEDKT
                     FQTEMEASEQEFVRAMDDDFNSAKAIAVLFDLNHKVRNQALELSQRQNAARMLVRLGS
                     VLGFFQHLATELGSSLPDLSKQLIELIIRQRAKARADKNWALSDQIRDELAVLGITLK
                     DTPQGCEWSYDK"
     assembly_gap    16917..17016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17050..17874
                     /locus_tag="PLO35_01215"
     CDS             17050..17874
                     /locus_tag="PLO35_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78310.1"
                     /translation="MNPKRKRYLIILIIVLAVAAGVWLWAQVSGPAFNRPESVAFDPV
                     SGDFLISNVGNGTIVMMDSTGSLGMLIDKGLSKPRGLKMLKDVLWVTDDTQVHAIDVA
                     AKKIVASIPIPGSVMLNDIEADELGALYISDTRANCLWLLDPLSKKLEKVTDKLMSQP
                     NGIYFDAPRRQMLIVSLGEKQPILAFNTKTRGFSVFMDTIYSRLDGIQSDDPGRIFFS
                     SWAEKAIYMIPQTQNRFEIYQKDLTSPADFYWHQPTNELIVPLMEKNKVIRVPLDE"
     gene            17867..18508
                     /locus_tag="PLO35_01220"
     CDS             17867..18508
                     /locus_tag="PLO35_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOH78311.1"
                     /translation="MNEPKSTTIELLTDRVIKHFRRPEEFYRELEVYKLGLEITPRLL
                     DNHEAVWIAISRVDGIPYLDSPDGFDPALLASTISALHSSIIKDGMCLCHIDNQPGNL
                     LWNGQRYYLIDFADSRLDLPETDITHLLLFWAEEFEPQRFRPLARNFLKAYRETTGMR
                     THLWDQEVWNQCLEQSRRRFYERRLRYGKFKPKLPESQRLANQELLDKLLTDI"
     gene            complement(18693..19376)
                     /locus_tag="PLO35_01225"
     CDS             complement(18693..19376)
                     /locus_tag="PLO35_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78312.1"
                     /translation="MIKELIILAASRKYGKFCVAGIDTRTGQWLRLVTTDSDAHYAID
                     ATDLIMDNGAQAAKLDIVSLEITDRSPSYFQSENYVIRKRSPWHYCGRASISDVTAIH
                     PPNRSEFIFYDTSRRLSRDFYRGLKPSEVQSLLLISPDSAQVEILEKEAKRRVLLHLH
                     YRQRDYEPLPVTDLEFCARCETLAPGLYPMRKHGIMLCSVGECYEKDQYHYKLVAGIM
                     LENGVNSKA"
     gene            complement(19373..19816)
                     /locus_tag="PLO35_01230"
     CDS             complement(19373..19816)
                     /locus_tag="PLO35_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOH78313.1"
                     /translation="MKLFTIGFAGKTAETFFTLLKAAKVKTLLDIRLNNRSQLAGFSK
                     AGDLEFFLKELGGIGYFHTSELAPDQELLEAIRSKEIGWDEYIRRFEALMESRKADKL
                     LRELLRELPGPLCLLCSEPKAADCHRSLVALRIRKLKPKTEIIHL"
     gene            complement(19816..20436)
                     /locus_tag="PLO35_01235"
     CDS             complement(19816..20436)
                     /locus_tag="PLO35_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOH78314.1"
                     /translation="MDIIFTIGHSNHETHQFINLLRGQGADLVLDVRSSPWSKRYPQF
                     NREELGRDLLRQGIGYEWFGDWFGARQTDERFYMPAGWLNYAWYTASDHFKEGIRQLD
                     QWLTAGRIPALMCAEKDPFDCHRAIMVSRALSLQGYEIRHILADGTVQTQAQLDERLL
                     DKYFPHREELDIFGLIDGETDPAQTLAEAYLKRNEAIAWRLENPED"
     gene            20847..23039
                     /gene="recQ"
                     /locus_tag="PLO35_01240"
     CDS             20847..23039
                     /gene="recQ"
                     /locus_tag="PLO35_01240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOH78315.1"
                     /translation="MQYSRQQVYKALARHFGFSEFLNAQESIVTGLLEGKSLLAIMPT
                     GSGKSLCFQLPAMIMEGYGLVVSPMISLMKDQVMQMRQLGIPAAMHNSLQSQEEQARV
                     RRELTDGKLKLLYMAPETLVRPQILELLEARPPSLIAIDEAHCISMWGHDFRPEYRQL
                     SILRERFPKLPCFALTATAIPKVRDDICAQLSIPPEHQVIESFNRPNLLLAVEPKKNS
                     FKRLIGFLSRHNGESGIIYCMTRKKVEDIANRLVLEGFRALPYHAGLSDTERHRNQEA
                     FINDEIPIMVATIAFGMGINKSNIRFVVHLDLPKSLETYYQEIGRAGRDGLPSSCLLL
                     YSYADVRTLNEIIYTDDETMNEITRRHLDAMLRYCKHEGCRRIPLLQWFGEHYKESNC
                     QMCDNCLADISEKMDASEQAKMFLSAIYRAQEKYPARRLIKILRGSKSKELLEAGDTK
                     LSVWGIGKQWSEVMWFSLFHSLKKDGSLKQEYPDFNLKLTPRGWEILKSQRSFTMPVS
                     LMEVLLKDKEEKIEDELFDQLSALRAEIASRQQVPPYVVFSDKSLREMSSFFPQSAES
                     FTKIGGVGTFKAQRYGDVFIRLISSYCLPRGIADNTSTKTSVPSKKATIGRTVEVVDY
                     LKGGHNLQETMVQFGIKLETLLDHLQRFLEADGWIDPDLLRSCCTLGEADAARVRNCF
                     RQLGTEALGQVYRALNEEIGYTDLKLQRMIMVAELNPGGQNPPPADNP"
     gene            23203..25611
                     /locus_tag="PLO35_01245"
     CDS             23203..25611
                     /locus_tag="PLO35_01245"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOH78316.1"
                     /translation="MNRILLLITLLMLGCQILLLAQVSDAGEPVSVTGADSLGQSASV
                     MDSLFYAADSVAYYYSQDKIELSGNARVSYQDSEISADSMYIDLEREQAFTHGSTIMK
                     DGEQILLGRDVSYDVDTQTGLLKEGISRIEKSYYGGVEVRKVGPKIYDVEEGTFTTCE
                     HAEPDFWFSAKQLRIYQGDKIVGKPVVAWVNHFPVFYFPFLTMSIRRGRHPGFLVPEP
                     GYNSVDGKFLRDIAWYYPIKDYADLILGLDLMERSGWKAKLSTLYTQRYEFDGNLSAA
                     YQKQVLGGNTTFDWSLRARHHHELGEKAAFDMDLDFISNKRVWESSNSLDESLAERLS
                     SSMSYRQPLLGSYLNVGALYTQDLVNDRATVSLPSASFSMPGQPVYELFLKPERSPNT
                     WWSNLSYNYSVRLDHVGEINDPKPGIMDILWNNSIDPIDSTLIRTRHNAGIRHSLGLS
                     YNFKLFGWLNTQQGLSYNEAWFDRDKNDDKWVRGNDYNASASANFNIYGIRNFRSGWL
                     SSIRHIVTPSASVSFKPDFSSNERFYSFGGIGLAGGEASSNLSLNLEQKWQLKYRSGK
                     EEKRLGDVLTMSSRASADLKSKERKFTNISHSLAFRPGSFNLGAFRIPGTSTFLKGIS
                     VNYGAQFSASHDPRDINFSSLGLRNQYFSHSFGLSGTAAYKTWFSKPKNQIFSPYEAA
                     DSLQTEAEDLASGGSDDGWKLSLSQDINAEKDIFDPRGSNLRLDASLKLSQNWLLSYS
                     NYYDVKNSDLISQSISISRNLHCWKLEMSYSRRNEFWEYRIALFNVVLPDALRFQTRD
                     SKRN"
     gene            25592..26182
                     /locus_tag="PLO35_01250"
     CDS             25592..26182
                     /locus_tag="PLO35_01250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH78317.1"
                     /translation="MTANAIRRAVFLDRDGCISPDEFGYISDPSSYHLYSYTGEALRM
                     LKELGFLLFVVTNQSGIARGYFGVEELERVLDRMRGLVSQEGVELDGVYYSPYFKDGI
                     VTPWNVEHEDRKPGLGMFRTALREHRFQASASWMIGDRYTDVEFGKKAGLRTILLLSG
                     NGWDELRTNILSWEYQPDLICEDLLIAARVIQKLAK"
     gene            26345..27154
                     /locus_tag="PLO35_01255"
     CDS             26345..27154
                     /locus_tag="PLO35_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH78318.1"
                     /translation="MKPLFHYQEKKFRALSKPAQLKAFTRLIRELERQSCQTDPEPKL
                     LEMIAKTAGWIETEDPLLKSFMDLASWGTDKRETALKAAAWLISQEAVPRDDAIPVRQ
                     NDGLRVSDPHFVERAQNMAVILSDLRSAFNVGSIFRTAECLGIGELWLCGITARPGEA
                     ALEKTAMGTTDKVAWRGFATAIEAVQTARKQGRIVYALETSGQAVSVFDASISFPCAL
                     VVGNEALGISPEVLKASDELLELPVMGWKNSLNVGVAFAVCAYQMIFKGAK"
     gene            27151..27951
                     /locus_tag="PLO35_01260"
     CDS             27151..27951
                     /locus_tag="PLO35_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOH78319.1"
                     /translation="MMEHLDFYRKICDCLAENCRLWQLSVVSCEGSSPAKPGMKLCVP
                     LQGREFGNLGGGEVEHSAIAWVRENRPSEPVLRTYMLSEQGDQALADEEIPTRMICGG
                     KVSILIEPLAKSRTLYIVGAGHCGRALGQLAKLAGWWVHLIDNRNELLESDLSLYGDV
                     ASHSDYSDVSTAISFDREAHIVIMTHGHAHDKQVLEQCLRREFAYLGMIGSASKVAQT
                     FSRLKEQGFSDAELARVHAPIGLPIGSHTPFEIAVSIMAELISIHKRK"
     gene            complement(28423..28911)
                     /locus_tag="PLO35_01265"
     CDS             complement(28423..28911)
                     /locus_tag="PLO35_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOH78320.1"
                     /translation="MKIHILVHSKTGNTTRFADAIASRLMEAGHSVNITPLATVSPVK
                     EGSVRAPQDIRFTNLPDISDCDLLMLGGPVWAFGPSPVIIAAVKQLGKLSGKTVVPFA
                     TMGFPFRCLGGNGALRAIAREAATRGAKIIPGGIARQMGGKLEGEIAALSAEIAERIP
                     RA"
     gene            complement(28908..30587)
                     /locus_tag="PLO35_01270"
     CDS             complement(28908..30587)
                     /locus_tag="PLO35_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOH78321.1"
                     /translation="MRHNHSLALLAGMLAVLLLGAWHPARAEDLRLDIMFSNDVHGGI
                     DRAQATFMNPEFPPQLGGGGSAATLIKHVRSLQSKQRESILLDAGDFFQGRPVGTVTK
                     GQAVMEYMNAIGYDAMTVGNHEYDIMDTELMETLRLADFPILSCNIVDRRTGEFPWYV
                     KPYTVISRMGVRIGIVGFTTTDTEKMSFPENIKNIDFISEKEAVQEWVNVVRAKEKVD
                     IVIVLGHAGLPYDIEDGYLSRYDAKGNPLSDKRYAVWGYDAQEIAREVKGIDLFIGGH
                     MHRGVPKPWIDPYTHTMVIQGYAYGSNLGWITLTIDPETRTLTGYESPAIREGSMITL
                     FEDQFIPDPEIGHTIAEQVAIAEQGMDEIVGSSAVYLSRTNVDAQSLMGNTITDAMRH
                     EVNADFAFLNLGGVRADIKSGPVTYRSIFDVMPFDNMVVSFKCTGEFLRKIIETRVEG
                     GRHGLVVSGVNVIYSKKRPNFDRVTSLKIAGKPWDKNAIYTCVTTDFLMQGNAGLNLL
                     VQVPQEDITNHQINLRDAIVNYFRKNSPVKTKIDDRWERNDNAQPTAEMLK"
     gene            complement(30594..32051)
                     /locus_tag="PLO35_01275"
     CDS             complement(30594..32051)
                     /locus_tag="PLO35_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78322.1"
                     /translation="MNQDRMNKWISAPAGMTILMLAVMILAAPSLAAWSITDSYFGNK
                     YDGFEARAFAMGGAGTFNDLRPFGIAANPANLTLIKSRVGFQGALLVNRAEDNRYIPL
                     YNSFDNYIDDAVYASNINFYDDYAGSGFASMRFGLLKLGLGGYYKPLLNFDGNYIEEI
                     RNNRNTDNDVYPEKIAINAIENEGSLNQAAGVFSFGYELGENLELNLGAEVSLLSGEQ
                     TQMKTIRWSQWAIDTFTDAVDNPAKVLPDYTLSTETELEGTQMKGGLSMRLNNRFGLA
                     ATYTMKSTLDRTGSTHELQDAHYQNAGIDISTPIAEDYILPSEARLGFLYQPRNIMRT
                     WFNLDVEYVKWSDVSEHFEDQVNLYAGVEHWVENRLPLRLGFQAVNGYLRELESDGSI
                     IAKRIISPKISAGTSFALTSFLQMDLGFGYTWREFEALDLFADSYYNDKTYSGNSTYA
                     LWPNSHITLADRGWENPDKVRENNISLNAGLTFNW"
     gene            complement(32065..34689)
                     /locus_tag="PLO35_01280"
     CDS             complement(32065..34689)
                     /locus_tag="PLO35_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOH78323.1"
                     /translation="MRKLLILLLALMSLGLLAAKVDLNTASYSELRQLDLTEKQARDI
                     LEYREYVSIFSSLFDLRQIPSIDQRTLLRIKDQVVVSLYQETDDVEARREEIRDLLER
                     LDSNEGASEGMADVWEDYLMTPQNVNRMHFDDLVSLPNVSAIDAVAILNRVARGDTIA
                     DMRDLRNSVGLSHYGYTNLRSYVYYREPPVKNRMLYDAQMQFFTRYFEEGSYEMYHES
                     FLRSDYGNSAVTIEHKKDLAYWGYFNLDELDPDIVLKLRARYGNNYKIGLMNYTGKGE
                     SSLLDRNATDIANDSKFYAGYENSQLPWLGNTRLKLYLGHYRATFGEGLVMENTDFYS
                     ARKTGFGFSKRIMGISPDLSRTQEFALRGAAVEINNPMVNVAVWASQDDKDAIMYVDE
                     DGNTVKENGKDKVFSYVVPSARFDNDELREAEAYWNAELQSGTAYAVPYVNLAFRKDA
                     LREKLWGTHVQVSPLIGTRLGFTTYTALYEKAHFMVPDYDDLRTLLVRDSYYYPKFKQ
                     MDAEIAGLYSTYKPGVYDRNFRRVIGFDGGTVIGNTSIQGEYAELTVTGKDHKLGDDP
                     SALLLSAYTQFENLYFITLYRDYDVGFDNPYSNAFSEHQRFDDTIIEKNVYTLTNPIL
                     ADVYQGSTQAQPERGVYFETRYKFNNYFTVGRSYLDIFERKTDGRRTARFQSELEFRP
                     LYQLGLRLRYKNQVNRIEDQAERGVSKTNEYTASIRTFLSNRDFLEFEYRYNTVLSPP
                     YTSLTNPANPGENSMAAAMTLMTGDYIGVNYTHNFNKNLKLQGSFLYWFSNGISHWDW
                     EDMEIDFMGEKGAKAWVALHSRVSENMYLSLKFRNKTYQTKEYSIREYNVPVEGENYY
                     QRVEHKENTIRLSLDYRF"
BASE COUNT         7944 a         9478 c         9723 g         7576 t
ORIGIN      
        1 tgaaagagac attatgaaca gccaggctgt gagtagtgtt ttagtgtttt tcatacttcg
       61 ttccttatct ggtgaggggg gatggatgtc aaggggtttt ttagtgagat acctgcggta
      121 ggtgaggaga tacttcgtag gtgagatact tcgtaggtga gatcgctgcg cgatgtgact
      181 ggagcggtgg ctttgacatc ttggaggcaa tcttcagtgc caccaatcct tcagtactac
      241 acggctcgaa aactccaaag ccagaaaact tgaaaacccc tatcccctaa aaccataaaa
      301 ctccctctgt aactctcctc tgtttcctcc gttactctgt agtgaaaaaa gaaaactctg
      361 tatctcagta gttttgcccg ctgatcacag ctcgcgaaac tccggagtcc aatccggggt
      421 gggtatcaac tcctgcgcga agacatgctc aaagcccagc tctgaagcgg tttccagcac
      481 tctggcgtag tcctccgctt tgatcggtct catcagttcc ggatactccg cggctttacc
      541 ttttggatag tactgggcca taatgctgag ggtgacggag gttccaatcc gggaggcaat
      601 cttccgaagc gctgcctcac tgccggagag ctggttggga agcaccagat ggcggattat
      661 gacacccctt tttgcgatgc catgcctgtc agttgccagg tccccgcctt ggcgatacat
      721 ctcagctatc gcgttcatgg cgatctcagg ataattcctt actccggaat actttgccgc
      781 gtagatgcca taagcatact tgaaatccgg cagccagata tccaccaaac cctccagcgc
      841 ctggataagt tccacttttt cataggcgtt gctgttccag accaccggaa tcctcaggcc
      901 tgacactttg gcttgacgca ggacgggaat cagctgagga gtatagtgcg tgggcgaaac
      961 gaggttgatg ttgagggcac cctggctttg caggtcaagc atcatcttca gcacttcatc
     1021 cgcttggtaa gcttggcccc agcccagatg agagatctca taattctggc aaaacacgca
     1081 gcgcaggttg cagtggctga agaagatagt gccgctgcct ccggatgggg ccacggccac
     1141 ggatggggtc acagaactgg catctaatgc ttccggaccg ctgaggactg gctcttcacc
     1201 aaagtgacgg gtggccagat cgatttccag ggatgaaccc gctccacacg ctccccgctg
     1261 agcaaacctg tcgattccgc aatcgcgagg acaggcctgg caggctttca ggtccagaaa
     1321 ctcaggcttc agagcttgaa agctcccggc agcagggcag ccatatcggt gatcacatag
     1381 ccatgcttgt ttccatagat cacgctcagc ccggggttga attcatgcag cacctgacga
     1441 caagctccgc agggcgggaa aggtttgtca gactccaccc agacggcgat ggccagatag
     1501 tctcgttcgc cttcactcac cgctttgaag acagcggtcc tttccgcgca gatagtgagc
     1561 gaataggatg agttttccac gttgcagccc agatagactt tgccgctgac acccaagagc
     1621 gcggccccca ccatatatcc ggaatagggt acgtaggctg cttgggcggc ttttttggca
     1681 tcgtccagca gagatagtaa aacgtctttt tccatattca gactcctcag gccaaaaggg
     1741 ccagaatcaa gcccccggcg atcacggaac caatctgtcc gcccacgttc acactcacgg
     1801 ccggcatcag gagaaagttg aaaggatctt ccttttgccc catctgatgg atcacgcggg
     1861 cactcattgg aaaggcagaa attccgcagg ccccgatcat gggattgatc ttggttttac
     1921 gaaacagatt gaaaagcttg gcgaacagca ctcctcccgc cgtatcaaat acaaaggcca
     1981 ccaggcccag catcatgata aacagggtct caggcttgag gaaatcctcg cctttcatcg
     2041 tggaggcgat cgtgataccc agcaggatgg taatgatgtt gcccagctcg ttctgcgcgc
     2101 ttttggagag gctttccagc actccgcttt cacggatgag gttgccaaac atcagaaatc
     2161 cgatcagcga gacggaatcc ggcaccacca atcccgcgat cagcgtgatg gagatgggaa
     2221 acacgatctt gacaatcctg ggaacgtcgc cgctgtgata gtccatgtga atgcggcgtt
     2281 cctgtttggt ggtgagcaat ctgatgatag gcggttggat gatcggcacc agggccatat
     2341 aggaataggc cgccacggaa atggggccca agagactggg agcgaagcgg ttggccacat
     2401 agatggaagt ggggccgtcc gccgcgccaa tgattccgat agctcccgct tctttcaacg
     2461 aaaaacccat taaagcggca atggccaggg tcacaaagat ccccagttgc gccgccgctc
     2521 caaacagcag catggaaggg tttttcaaga ggggggtaaa gtctatcatc gcgccgatgg
     2581 cgataaagat cagcagcgga aagagctccg tgcggatacc ggcattgaat aagatcgtga
     2641 gggccccgtg atctactacg gcggctgaaa agggaatatt ggccagaatc gtgccaaagc
     2701 cgatcggcag caagagcgcc ggctcatact ttttggcgat ggccagatag atcagcaccg
     2761 ctcccacgat catcatcacc gcctggtccc agtaaagatg cgtgaggcct cgtgttatag
     2821 cttccatcag atgactccat tggtggatcg gcaaacgtcc ccgtctgccg tttcgttaat
     2881 aatccccacg ccatttagcg caggtcggac atctgatttc aagtgtgccg ctcttgtcaa
     2941 ctactttctg ctcacctgca ctcctcccct actccaccta tgccaaagca aaggagaggc
     3001 aaaggagatg taaaggtgga gtaaaggagg cgaagcgcta aggattgagc aacagctttt
     3061 tggcgccttg gaagttgccg ctctccagct tcaacagata aactcccgcg gcacaaggat
     3121 gtccgctttc atccagtccg ttccagaggc tttgatgagc accggaagcc agattggcag
     3181 agatcagagt cctgatcagc tggcctttca tattatagac gcgcagtttt gtggatgccg
     3241 ctttgggaag ctcgaagccg atgtcacagc gatcccgcag gggatttggc caagtcttca
     3301 gagccagttg gggcacaggg atttgaggat cgtcaatggc aaccgcgcca ggcctgatga
     3361 tctgggtctc gctttccaga tagacctcat ccgcgcccac aaaatagatg gaagcgatcc
     3421 tgaactgata ccagccgtct gaatcgaggc cactgatagc gtgccagcgt tcggaccccg
     3481 ctttattggt ccttacccag tctgaagagg gagcgtccag gagtttgtac atcacctcaa
     3541 agtgatccgg atcggtgtag gaatgccagt tcagttccac accgccctcg ttgacgatct
     3601 ggaccggcat cagataagga atgcaggtga gtcccgcgct gggattgatc tgcagattct
     3661 gctgagtgat aaaggtttcc agactagccg ccaggaggtt gagatcactg atctgactgc
     3721 ccacgaagtg cacagaatac cagctaaagc tctgtccggg agccagatgc acacggccag
     3781 gcggattggg ctgagcggcc tcaggttgag atccatagag gcagtgcaaa aagcgggtat
     3841 cgttcgccgc gggctgttca tactggccat ccggtgactg gggaaacagg ctcacatatt
     3901 tggtgtaatt ggcattacgg ccggtgagca gccagtattt ttcgttgctg gtgagattgg
     3961 gtatgtagtt gaagccgcgc ggatcacgat cgtccgggcc gtctccaact ttccagaacc
     4021 aggcgttccg attgccgtta aagcctggaa tgatcagctt gttggccagg atgtagggac
     4081 tgaggcctcc gtcatgatca aaatcccggc tgtaggcata atccaggccc gcgcccctca
     4141 cataaccgct tgcgtcatcc gcggcaccct ccactccctg gccggcggga tggacatcgg
     4201 catccacata ttctccaatc gccagatcaa atagagtatc tatggggctg gaattgatca
     4261 tagtgtattt atagatgacg gccctgtcgt agttttgcag gttccaggca tagctctcgc
     4321 gatggatggc caggcccagc ggataatggg tgttcatgtt gtgggaagct cccagatcgc
     4381 cattcgcgta ggcatagagt ggcgagtaat cgtaaaacca ggcactcacc gtctcgaagc
     4441 cgggagtgac gaatgtgccg ccctgcggca aactgaagca gtaagtttgc tggggatcgg
     4501 gaattacaaa gggacgggga gcgggagaac ccagaatgct tttcagggct acatccgctg
     4561 aattgaagag gctgtattgc gggctgttcc atgccagagg attgtaggcc ggcagcagtt
     4621 catagaggtc ctgatcgcca tcgaagccca ccgtgcagag ggtatccacc acgggtttga
     4681 ggctggaatt ccagccagga tcgaactgag tgaccattcc agcgttgaat tggtccggat
     4741 tgggagacag ccagtaatac agatggccgg atgggtctct gcgataacgt tttgcgccta
     4801 tccagggggc tgaagcatac atgagcttgt gctggcgcgt ggagggatac agcagattgg
     4861 tgctgaaacc gtagttggag accgtcaatc cgatactgcc ggaattgtgt gtgctggtgc
     4921 tcatggcacg atccggatca acaggctgga tagataaagc ggccagggct gagacggcca
     4981 ggaaaacgat gaaaagatag aagcgattca tttgtggcct cctgaagcgt aactggttcc
     5041 cagccatgcg gacagcaggt cacttaaggc ctgggacaca tccgcgacct ccatatagct
     5101 gagcggaaag ccaaacacgg ccgtgattcc catatcctgc tgatgggcga aactgacgta
     5161 tttggatgaa aatgtatcat actgatcttg attgggagga gagacggtag atgtaaccgg
     5221 tttacagaca aagcgatgca gctctccagc ctgctgactg ccatcgtaac ataatactcc
     5281 tccaatccct tgccgcgttt ccaccacgcc caccatcggg tcctccagtt ccagatcgat
     5341 atcggcgatg ccatccaagc cctccgctcc tataaaccaa ggatttgaga taacggaggt
     5401 gctgaggagg ctgaagcagt tcaggttgct gataccaaaa cgctgggtga ccaactcggg
     5461 atagtggtcc ataaaatagt tgagcttgct gttcaaccag tgattaccgc tcaggatgag
     5521 cttaccgcct ccgtccagaa acacttccag ggcgtcgccg tagagattga tatctccaga
     5581 ggaggaggga ttttcctggt gccagatcac ggccttgtaa ttctggagca gagcgggatt
     5641 cagagcggcg ttccagggat cattataaga aatctcatgg gtctcgacgg gaccccaggt
     5701 tgtaggtacc accgaggcgt aaaacgcgtc cacaagcgct tcgggagtgt aggtgctgtt
     5761 gttgtaggtg ccgtccacga tcaggatacc gttgcgcagt ggaggtgctt tatagggcac
     5821 gagattgatg gtaatcaccg cgggagtttg atcatagcgt ccctgtgcgt ccacggcgca
     5881 gactttgaag atatgaggag cggcactgag gccggaaagc acacaagcgc tggcactgtc
     5941 acctatattg cgtaccctga gccacataga gccgtccgcg tgggtcacaa actgaggcgc
     6001 gataaactga ggcaggacag gaaagcgctc tccatccaga ctgaggtcat aggccacgat
     6061 atgaccgtga tagtcgacgc cgctttcgct gacgcaattg tctaagtggc tgtccagggg
     6121 attgttggtc acctgggcat tgccgttgaa ctgatagcca tactggccgt tgtaacccca
     6181 gtgcagatga agcttgaaat tcggggaatg aacggcggta tagacgtttc ccgtcttcca
     6241 gaggatgccg ttgcggcgat acgtctgaga tggaatcagt tccatatcat agatttgctc
     6301 attgcggttg atgctgtaat gatgatctcc cagtccggcg atcagattgc tgtggatcac
     6361 ggcatcggga tagaaatccc catcggcgtg gaaatagacg cttttgggag tggcctcttc
     6421 cacgccctga cggctcacca cataagcctc aaactgggtg tagcgggtat tgggagtggg
     6481 ctccagagcg ggaagcgtgc tttcattgag aatcacctgc cggatatcgg gtgaggtgat
     6541 ggaactatac cactcgccgt gggatatgac gtcgaggctg gaattgatga tattgaggcg
     6601 atacataaag tgatcggcaa tcaggccttc cacaaagata tcccccacgc tctcggccag
     6661 gttgaaacag agcccggtgg aggtggtggc gccgtttgga aaggtgacaa agatgtggct
     6721 ggatacgatg cgctgggtct tgaagggaga gtggaactcc gtgatctggc cattcggggc
     6781 tttgattctg agcgtggcgc tggtgatcag gtcggtcagc ttgccgtttt gcgatctgta
     6841 gtccagattc agttccttct cagaagtcca gatgctctgt ccatcttccc tgagcagcca
     6901 tccatccgga tcaagctgag atcccgacgg cggttccccg ctcagagtat gcagtttcca
     6961 ggcatacagg ctaccctggt gactgtcggt gccagcacaa aggctttggc gatagatata
     7021 ctcatctccc cccagaggca gcttgtcttc cgagcagata atgtccgctg gctgggccgg
     7081 tgtgggtgtc aggggcaagg gctcctgaca ggaagtcatc aaggccagca gaagcggcaa
     7141 ccacaggatc actgggtaag ttttcatcct atcacctcat atcattgatc tgctacaagc
     7201 ttttctgtct tgtcaattga gtcaagtgtt ttttttatga tacttgcaag caaactttcg
     7261 cctcgcggcg gggatttgtg gattctcttc cacacggata aataactctt atactctctg
     7321 ccctggagaa cgtggacggg ccccccgagc gataatccta agttccccct gccgtgataa
     7381 ataaatagat acgccaaagg aaagcttgac aaatatcagc tatccacaat catgagtttt
     7441 atcctaccga ttgagaggat tatatggcag tatgggagct taccgtcact ggccgggtcc
     7501 agggcgtcgg gtttcgctgg tacgcgaagc acctggcggt ggaactggga gtgaagggtt
     7561 acgttcgcaa cctgccggat gggtccgttc gaatcctggc ccaggcggat cagctcaccc
     7621 tggaacgctt tggaactgag gtcagaatgg gcaaccggca tgccctcgtg gcagaggtcc
     7681 atatcatgac tctcgatcac gcaagtgaat attatgattt tgagatacga taaaacccat
     7741 agactcaagg ttcgggctgt ttcgtggcat ccgaccttaa cggcctccgg caggaatcgt
     7801 ccgcttttgc tgatgctgct gttcttgctg ccggtgctgc tttttgccga ttttgaatac
     7861 cacagcgtca agcaaggcga cacactctat ggtcttggca agcgatacgg tgtgagtgtg
     7921 gcccagatca agcaactgaa caatctctcc tcagacaatc tcagcattgg acagaagctc
     7981 aagatcaaag agaagcccaa accccaagcg aaaccgaaac ccaaaccggt tgccaccgct
     8041 gagcccaaac ctgctgttcc cgctccggtc acgatcaccg ctccgccacc tcccaagcct
     8101 gctgctccag tcgcggtggc cactccccaa ccggaaaacc cgggtccgag tcccgcttcg
     8161 ggacagcttg ccgtgcccgc gcaagagctt cccaaagaat tttatcacat cgtgcagccc
     8221 ggtgaaaacc cctaccggat atcgcagaat tatgggatca gcactcagaa ctatttcctc
     8281 tggaacggta aacagagctg ggacgaattt gacatccacc ccggcgacaa gatcatcatc
     8341 aaagatcccg ctctggccaa acctggatcc cggtctgaag tgccaccggt gcagcaaccg
     8401 gaagcggttg cctctccttc tccagctacc cccacggttt ccgcggccag ggatacagtg
     8461 gtgatcacca agacctatac cgtgaaaccc aaggacactc ttttcagcat ctccagggcc
     8521 aacaacatga ccgtggatga gataaaacgg atcaacaacc tcagttccaa cgctatcaat
     8581 gtgggccagg tgctctatct ggccggaact cctccatccg gtgctcccag acaatccact
     8641 cctcccagga ccgaggccga gctggaagcc aaagacgtgc tccgcaccga cctctttacg
     8701 cccgtgcagg gcaaggtggt atctgaattt ggcattcgca acggaaaacc acacaagggg
     8761 atagacatct ccgccaagac cgggacgcct atctattccg cattggatgg agtggtggtg
     8821 ttttccgggg tgcagggcaa ctatggcaat gtgatcgtgg tggagcatcc cgattttgtg
     8881 atgacggtgt acgcccacaa cgatcaaaac ctggtgagcg tgaacgatac ggtcaaacgt
     8941 ggtcagatga tcgcctatat tggcagcacc ggaaacgcca ctggtccgca tctgcacttt
     9001 gaataccgca tcaaaggcaa agccatcaat ccgcgcaagg tgctgagttt atagccatgc
     9061 ccacaggtat gtcctcctcc aaacgggaaa gcgtattcgc ggacaaagcg ctgcaaaact
     9121 atctgcgcga gatttccaaa tataagaccc tcagccggga agaagaacac cagttgggca
     9181 tccgggccaa aaacggagac accgaggccc tgaaccgcct catccagtcc aacctgaaat
     9241 ttgtggtcaa gatcgccgct cgttatcaga atcgggggct cagtctttcg gagctgatca
     9301 gcgagggcaa catagggctc atcaaagcga tcgaaaagtt tgaccccgat aaagatatca
     9361 agctgatttc ctacgccatc tggtggatca aacagcggat catgctggcc gtctcggaaa
     9421 agagctccct catccgcgtc ccacttggca agaccagcac cgcccatcgc atcaaagccg
     9481 tgcaggaccg cattttctcg gaaacgggag aatcggccag cagccaggta ttgggtgacg
     9541 ccaccaatat gtccgccaaa tccgtggacc agctcaaaga ccagatcgtg gagactacct
     9601 ccttcgacga gatcatccaa agcagcgatt acagcgaata tagcacccgc gacctgctgg
     9661 aagacaaaga caccatcgat ccccagcagc tctatcaccg cgagcgcatg caggagcgcc
     9721 tccacaacgc catcgacaag ctcgacaacc gtgaagcgga tattatcaga gactattttg
     9781 gcctcaatga aaacaacgaa gcccaaaact ttgcccagat cgccgatcgg atcggccttt
     9841 ccagagaacg cgtccgtcag atccaaaagg aagccctgcg taagatgctg gctgagctca
     9901 aaccagatga ggacgccttt gtggacgagt ttatcgaccg ctacggttga gccggatttt
     9961 aacagcacag gagagataga tgtttcgcaa ctttgatgag atgaaagact atgtaaaaca
    10021 aggccgcaga ggtagagtgg tgattgccgc cgctcagacc gacagcgtcc tcgacgcagc
    10081 cattctggcc gctcaggaag acctggcgga ttgcattctg gtaggcgacg cgggggccat
    10141 cagaaagctg ttggagacga tggcacccga caaggtgtct gcctttgaga tcatcgacac
    10201 cggggccgac ctcgtcaaag ccgcccgcac cagcgttgcc cttgtgaaag aaggcagagg
    10261 agacatcatc ctgaaaggca agaccgatac gtccgtgctg cttaaagccg tgctggacaa
    10321 ggaaaacggc ctgcgggtga gtgaagtgat ctcggacgtg ctggcctatg agcatccaga
    10381 cggcatccgg atgatgagcg acggcgggat caatatcgct cccgagctca acgaaaagat
    10441 cgccatcgtg cgcaacagcg ttcaggtggc ccacgccatg ggaattccca atcccaaagt
    10501 agcgctgctc tcagccgtgg aaaccgtcaa tcccaaaatg ccctccactc tcgacgccgc
    10561 catcatcagc aggatgaaca aacggggaca gatcgccggc tgcagcatcg agggtccgct
    10621 cgcttttgac aacgcggtgg atatcgaagc ggccagaatc aaaggcatcg tttccgacgt
    10681 ggcgggccag gccgacatcc tgatcgtgcc caacatcgag gccggcaaca tcttcggcaa
    10741 gaccctcacc tattattgcc attaccgcgt ggcccatgtg gtgatgggca cccgggcccc
    10801 catcctgatc ccctccagag cagacgacgg cgagaccaag atgctctgca tggtgatggg
    10861 actggcctcc atgacccgct gaccggtgtc cgcgagcatc ttgtctgctc ccctggcaag
    10921 ctcggttacc gctcctccgg tatgaatctc gcgctcgttc agatcggcaa gaccaaagat
    10981 cgctggctgc aagagggcat cgatgaatac ctcaagcgtt tgggacccta ccataacctt
    11041 aacatcatag aactgccaga ccactcccgg ctcaacagcc aaaatagcga gttggtccag
    11101 tccagggaag ccgatgccgc gctcaagcaa ataggagagg atgactatct ggtgctgctg
    11161 gatgaggctg gccagctcaa atcttcgctt gaattttccc gctttttgac tagtttatct
    11221 gataaacggc gggtggtgtt tttgatcggc ggagcttttg gcacccacaa gcggctcaaa
    11281 gatcgcgcca atcagatact tagcctttcc ccgctcactt tcacacaccg catggcccgg
    11341 ctgatcctga tcgagcagat ctatcgggcg acgatgatcg cccaaggccg cagctatcat
    11401 atataaagga ctttttcaat gattactctg ataaccaacc ccacgatgag caacttcggc
    11461 caggccctgc tgatcctgtt gcaggggatg acgggcatct tctttttcat ggccctcttc
    11521 tatctcctga tcctgggcct ggaaaagctg ttcaaaccca ggaccagcca atgaaacgcg
    11581 ctcttaccac attgctggcg ctgttgatgg tcctcagtct gccagctggc gtcaccctgc
    11641 tgcaaaccac gggcagggac ctcacgctgg aataccgtct ggacccctac aagctcatcg
    11701 aagagaacgg attcatcacg atctcgatgc ccggcatgga ttatcccagc ctgagcggga
    11761 gcccgctgct gccatatctg gaaagcaaga ttggcctgcc accgggaggg cagatggctt
    11821 gggacctgca aagcgtggaa gaagagcata tcactctgaa acagcgcctt actcccgctc
    11881 ccactatgaa gtttgtaaac gatatgtccc gctcggacta cctcgtcgat gaagcgctct
    11941 atgccgccaa tccagactat accaccgctc tggaagctca gaactggcgg ggtttccgct
    12001 tcgccgctct caggatcact cccttccgct atgatggaca gaccaggctc actatcctga
    12061 aacgcgccgt cttcagcatc cggattcagg gggacttgga gtatagagaa caggccggga
    12121 ttgatgaatt gatggagttg atgctggggc aattcgtcaa cggcgagcaa gccaaagcct
    12181 ggcagagttt tagccgcaat cccatcaact acgccccctt tgcatcagcc gaccaatggc
    12241 tgaagatcga aacagacaag gaggggatgt acaagctcag ctatcagcag cttagcgctc
    12301 ttccgctggc agatatcgac cccaggactt tccggctgtt cagcaccggc ggcgagttga
    12361 gaccattcca ggtggtgact cccggtccgg aatttcaaga gctggccatc agagtcgtgg
    12421 gcgaggaaga cggccatttt gacagcggcg actatattct gttctatggc agcagccggg
    12481 acggaaccct caaaaaccag agcctcgatg tggtgcccac ttccttcaat ccctattccc
    12541 aaaacagcgt ttactggctc agctttgggg gcagtttttc aggtccgccc cgtcgtatgg
    12601 acactctgcc tcagcccggc agctacacca aggccgtcca cactcaactg gcccagagca
    12661 ggtatgagac ggaaactcac cgccgcaccc agatcggctt tatatggtac agcagcaggc
    12721 tgtttggcaa cagcaccgcg gagtatcaat acactctgcc gctttcgaat ctgaacgctc
    12781 gcggggattc gctgctcacc atcaggttca ggcaggaaga gatcgattcc gatatctggc
    12841 actatatcaa cgtgttcgtc aacgatatcg aagtagctcc cccctccgga caaaccagct
    12901 tttcctggca aggaacggga gaatatatct tccagaagta tgtgaccagc tttgtaaacg
    12961 gcgataacgt catccgggtc cgcgtcaacc gcaacggcac ggacaatctg tttctggatt
    13021 tcatcgcggt ggattaccct cagaccctga tcaagggggt cggccagtat ctcattaccc
    13081 cggatcccac cctgatttcg cagacggtgc gctacaccct gccgggcggt atcggtgagg
    13141 cggaagtctt tcgcgcgctg gactatgcag acgtcagcaa ggtaccggtg gtctctgaac
    13201 ccgattccct ttggtttgcc gcttccggga ccaacgccag ccgcttcata atcagcactc
    13261 cctcagagta ttacagcccc gtaagcatca ctgaggtcaa tccggtggac ctgaccgtca
    13321 gtcccagcca ggtagaccac gtgatcatcg ctcccgctga gtatctgact caggcgcaga
    13381 cgcttgccga tatgtatgcc gatttccacg ggcttagcgt caaggtggtc gatcagcaag
    13441 acatcatcac ccaattcaat ggcggacatc ccgatccggc cgccatccgt cagtatttgc
    13501 gctacgtcta tcaccatcac cccgctccca gattgagagg cgttaccctg atcgggctgg
    13561 ggaccttcga ctggagaaat caatcccgcc aatccagcgc caaaaacaag ctcatgatct
    13621 atcaacgggg agaaagcagt tcggacgatt tctacgccat gatgagctcc tccttttatc
    13681 cggagctgat cataggccgt tatcctgtgc gtaacaccaa cgagctcaac aacatgcttt
    13741 ccaactaccg cgagtataac cagaatcccc agggcggctg gtggagaaac tcgatggtga
    13801 tgctgggaga cgacctctac aacggcagcc ttgaccacta cgaaaacatc catacccgtc
    13861 aggtggaact agctgggaat gtagtccatc ccagcatcca ggtggacaag atcttcgcct
    13921 gggaatacga ttacgatgaa taccaaaaca aaccccaggc ccgggacgac atgcttgccg
    13981 ccgtcaatga aggccgtctg gtgtggtatt acatcggtca cggctcattc gactcactgg
    14041 gctcggagga ttttttcaac ggcgctaccg atatgggcag attcaacaat gccaaccatc
    14101 tgcctctgtt tatggccgct tcctgcaagg tttcgcagtt tgaccactgg ggcttcgaga
    14161 gcctgggcca gaaagtggtg atgctcaaca atctgggtgc catagcttcc ttttcggcca
    14221 cccgcctcag ttccccctat tccaacgcgc ctatgggaga aaagattcta gacaacctgg
    14281 ccaataagcg caactatctg ggctacagca tcatggccgc caagctggct tctcagacgg
    14341 acgataacga ccacgtctat gtactactgg gagatcccac cctccggatc atcccgcccc
    14401 agcgggacag cctgatgagt gtttccaccg gcatcgccag agacggcaag accaccctgc
    14461 agtcccgcca gaaagtggat gttcagggct cgctgaatcc ctacagcggt gcaggtacag
    14521 ccgagatcaa ggtctttgac agtgccgtgt tttacaacct ggacgcccaa tccagagttt
    14581 cacaccgcgg caccactctc tttaaaggct ctgccactgt cagttccggg cagtatgagg
    14641 ccgctttcat cgtgccggac gatatcacca acggagacac cggctcgatc gtcagctacc
    14701 tctgggatgc acaggcccag cgggactatg tgaattacca ctatcccgtc acgaccagtg
    14761 accaggcggt tcaggcggaa aacctttccg ctcctgccat tgagctgttt ttgggctctt
    14821 atgacttccg gcccggggat accgtctcca ccagccccac cctgctggcc aggatcagcg
    14881 acgacaacgg catcaacctc acaggcagcg cggggcacaa tatcctgctg gtgatcgaca
    14941 actccatcca gccgatcccc gtcacctcat acttcagcta cgatcaggat tcctacacca
    15001 gcgggattct gcgttatccg atatccggcc tcagcgaagg tcctcacagc gtgcaggtgg
    15061 tagcctttga caactttaac ctgccctccg tgaccagcac ccagttcatc gccaaaaaga
    15121 ccggagcgct gtcgatcgag cgccttctga tctaccccaa tcccattgag aatgagggac
    15181 atatcacctt catcctctct gaggacagcg atctggatat cggcatttac accatcaccg
    15241 gcaaacgtct gcgtcgcatc caaactcccg gacgccaagg ctttaaccag attccatggg
    15301 atgggcgcga ccacaacggc aaccggcttg ccaacaacag ctactttgta aaagtcacgg
    15361 ccaaaagcgg ctcccgcacc gcggaagcaa gggaacgcct ggtcatctat aaataggaat
    15421 atcgatgcaa atctacaaca ccgccaccca tcgcaagcaa gaattcatcc ccgtcaaacc
    15481 tggcaaggtg agcctctacg cctgcggtcc caccgtgtac gactattttc acatcggcaa
    15541 cgcccgcgcc tttctctttt tcgatgtcgc ccgccgctat ctggaatacc gaggctatga
    15601 cgtcacctat gtgcaaaaca ttaccgatat agacgataag atcatcgcgc gctccatcga
    15661 agcggggatt ccctttagcg agtttgccgc caaatatgcc agcgcttttc tggaagacag
    15721 cgccgccctg gggatcaagc ccccgcacca tcaaccccgc gccacccaaa tgatgcctca
    15781 gatcatcgct ctgatcgagc agcttgtcca gcacggctat gcctatgagg tggaaggaga
    15841 tgtctatttc gacacggccc ggcttccgga atacggacag ctgagcggca agaagatcga
    15901 agaccagctg gcgggggcca gagtggcgga aaacaccctc aaacgcaacc ccaccgattt
    15961 tacgctctgg aaacacagca aaccgggtga gcccatctgg cagagcccct ggggcgaagg
    16021 acgtcccggc tggcacagcg aatgcgtggt gatgggccaa cactatctgg gacagacctt
    16081 tgacatccac ggcggcggag tggacctcgt gtttccgcat cacgaaaacg agcttgctca
    16141 agcttacgcg ctgtccggcc aaccgctggc caactattgg gtgcacaacg gcttcctgaa
    16201 catcgaaggc gagaagatga gcaagagcct cgataacttc tttacagcca gagacatcct
    16261 ggcccagcac agcgctgagg ccatcagatt ctttttcctc tcaaaacact accgcagccc
    16321 catcgacttc aaccgcgaga ttatcgccga atccgaacgc gccatcaaca acttccgcga
    16381 atccctcaag gccattgact atctcaagct gccggaggac aagactttcc agaccgaaat
    16441 ggaagccagc gagcaggagt ttgtccgcgc catggacgac gacttcaaca gcgccaaggc
    16501 cattgccgtg ctctttgatc tcaaccacaa ggtccgcaat caggccctgg agctctccca
    16561 acgtcaaaac gccgcgcgca tgctggtcag actgggcagc gtgctgggct ttttccaaca
    16621 ccttgcgacg gaacttggca gctcccttcc cgatctcagc aaacaactga tcgagctcat
    16681 cattcgccag cgcgccaagg ccagagcaga caagaactgg gcgctctcag accagatcag
    16741 agacgagttg gccgtcctgg gcatcaccct caaggataca ccccagggct gcgagtggag
    16801 ctatgataag tgagagccgc gctgtaagat gctgcgcagg tgagagaccc tcataacgct
    16861 acacgcgtaa cgcttaacga tcttaggagt gacaatgaac cccaaacgta aacgctnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncacg cgtaacgctt aacgatctta
    17041 ggagtgacaa tgaaccccaa acgtaaacgc tatctgatca tactgataat cgtgctggca
    17101 gtggcggctg gagtgtggct ctgggcccag gtgagcggtc ccgctttcaa ccgtcccgag
    17161 agcgtggctt tcgatcccgt cagcggtgat ttcctgatct ccaacgtggg aaacggaaca
    17221 atcgtgatga tggacagcac cggcagcctc ggaatgctga tcgataaagg cctctccaaa
    17281 ccccggggcc ttaaaatgct gaaagacgtc ctctgggtga cggatgatac ccaagtgcac
    17341 gccatcgacg tagccgccaa gaaaatcgtc gccagcatcc cgatcccggg ctccgtgatg
    17401 ctcaacgaca tcgaagccga cgagctggga gcgctctaca tcagcgatac gagggcaaat
    17461 tgcctctggc tgctggaccc gctcagcaaa aagctggaaa aggtgacgga caagctgatg
    17521 agccagccca acgggatata tttcgacgcg ccccggcgcc agatgctgat cgtgagcctg
    17581 ggcgaaaaac agcccatcct ggccttcaac accaaaaccc gcgggttcag cgtcttcatg
    17641 gacaccatct attcccgtct ggacggcatt cagtcggacg atccgggccg cattttcttc
    17701 agctcctggg cggaaaaggc catctatatg atccctcaga ctcaaaaccg ctttgaaatc
    17761 tatcaaaaag acctcacttc ccccgctgat ttctattggc accagcctac caacgagctc
    17821 atcgtacccc tcatggaaaa gaacaaggtg atccgcgtcc cgctggatga atgagccgaa
    17881 aagcactacc atagaactgc ttaccgaccg ggtgatcaaa cactttcgcc gcccggaaga
    17941 gttttaccgc gagctggagg tctataaact gggcctcgag attacgccca ggctcctgga
    18001 taatcacgaa gcggtgtgga ttgccatctc tagagtggat ggaatccctt atctggacag
    18061 cccggacggc tttgatcccg cgcttctggc cagtaccatc tccgccttgc atagctcgat
    18121 catcaaagac ggtatgtgcc tctgccacat tgacaaccag cccggcaacc tgctctggaa
    18181 cggccaacgc tattacctga tcgattttgc cgacagccgt ttggaccttc ccgaaactga
    18241 catcacgcat ctgctgctct tctgggcgga agagtttgaa ccccagcgat ttcgacccct
    18301 ggccagaaac tttttaaagg cctatcgcga gaccacgggg atgagaactc acctgtggga
    18361 ccaggaggta tggaatcaat gcctcgaaca gagccgccgg cgtttttatg agcgtcggct
    18421 ccgatacgga aaattcaagc caaagctgcc ggaatcccag cgcctggcca atcaggagct
    18481 gctggataag ctgttgaccg atatatagca aacgaggacg tttgccgccc caaattcgct
    18541 ccatctcctc ttccccactt tcactccacc tgtgccaatg caaaggagag gcaaaggagg
    18601 ggcataggtg atgtaaagga ggctaaaggg agcgtaaggc cagaaactga tgaattgatg
    18661 agaattgatc cgatagaagc tctgccccaa gttcaagctt tggaattcac cccattttcc
    18721 agcataatcc ccgccactag tttgtaatga tactggtctt tttcatagca ttcgcccacg
    18781 ctgcagagca tgatcccgtg tttacgcatg ggatagaggc cgggggccag agtctcgcag
    18841 cgggcgcaga attccagatc ggtgacgggc aggggctcgt aatcccgctg tctataatgc
    18901 agatggagta gaacgcggcg tttggcttcc ttctccagga tttccacctg agcgctatcc
    18961 gggctgatga ggagcagaga ctggacctcg gagggtttga ggccgcggta gaagtctcgg
    19021 gacagtcgtc tggaagtgtc ataaaagata aactccgagc ggttgggagg atggatggcg
    19081 gtcacgtccg agattgaggc cctgccgcaa tagtgccagg gactgcgttt gcggatgacg
    19141 tagttttcgc tctgaaagta ggagggagag cggtctgtga tctccaggct cactatgtcc
    19201 agtttggcgg cctgggctcc attgtccatg atcaggtcgg tggcgtcaat ggcgtaatgg
    19261 gcgtccgaat cggtggtgac gagtcgcagc cactgtccag tcctggtatc gatccccgcc
    19321 acgcagaatt tgccatactt gcgggaggcg gccaggatga taagttcttt gatcacaggt
    19381 ggatgatctc tgtttttggc ttcagcttcc tgatcctcag ggccacaagg gagcggtggc
    19441 aatcggctgc tttgggttcg gagcagagca ggcagagcgg tccgggcagc tcacgcagca
    19501 attccctgag cagcttatcc gctttccggg attccatcag ggcttcaaag cgcctgatat
    19561 actcgtccca gccgatttcc ttgctgcgga tggcttccag cagttcctgg tcgggggcca
    19621 gctcagaggt gtggaaatac ccgatgccgc ccagctcctt cagaaaaaac tccagatctc
    19681 ccgctttgct gaacccggct aactgactgc ggttgttgag ccttatatcc agcagggtct
    19741 tgaccttggc ggctttgagg agagtgaaga atgtttcggc ggtttttcca gcgaagccaa
    19801 tcgtgaaaag tttcatcagt cctccggatt ttcgaggcgc caggcgatgg cctcgttgcg
    19861 tttcagatat gcctctgcga gcgtttgagc ggggtcagtc tcaccatcta tgaggccaaa
    19921 gatatccagt tcttcgcggt ggggaaagta tttgtccagc aggcgctcat caagctgggc
    19981 ctgcgtctgg acggtgccgt cggccaggat atgcctgatc tcatagccct ggaggctcaa
    20041 agccctgctc accatgatgg cgcgatgaca atcgaagggg tccttttctg cacacatcag
    20101 agcaggtatc ctgcccgcgg tgagccattg gtccagctgt ctgatccctt ccttgaagtg
    20161 gtccgaagcg gtgtaccagg cgtagttcag ccatccggcc ggcatataga agcgctcgtc
    20221 cgtctgccgg gccccaaacc agtcgccaaa ccactcgtaa ccgataccct ggcgcagcag
    20281 gtctctgccc agttcctcac ggttgaattg gggataacgt ttggaccatg gggaggaacg
    20341 gacgtccagc acgaggtctg ctccctggcc tctgagcagg ttgatgaact ggtgagtctc
    20401 gtggttggag tggcctatag tgaagatgat atccatggcg ccgccttgat gtttgataat
    20461 ccatgctact gttctgacgc ggtagaaagt gtcaatattt tttactgtat ggacggagag
    20521 aggggttgag ggggttgagg gggttgagag ggtttagagg gtttagaggg tttagaggac
    20581 acagagtgtc aaacggggac gtatgccgtt ccacaagcct ctctctgtgt gctctgtgtc
    20641 ctctgtggtg aataacctct gcttttctct gtcaaacctc tgcttttctc tgtgttaaaa
    20701 ttgtaacagc cgctgtccga gttggcaaac ggaaacgttt gccgttccgg gagggactct
    20761 caaccgaatt attgcttgac tgctggaagg tgttcacaga acctgcttcc gtttggtttt
    20821 atacttgtgc agttaaggag attcggatgc agtattcaag gcaacaggtt tataaagcac
    20881 tggccaggca ttttggcttc agcgagtttc tcaacgctca ggagagcatc gtgacgggct
    20941 tgctggaggg gaaatcgctg ctggcgatca tgcccaccgg ctcgggaaaa tcgctgtgtt
    21001 ttcagcttcc tgccatgatc atggagggat acggactggt ggtatcgccg atgatctcgt
    21061 tgatgaagga ccaggtgatg cagatgcgcc agctgggcat cccggccgcc atgcacaaca
    21121 gcctccagag ccaggaagaa caggcccggg tgcgcaggga actcacagac ggcaaactaa
    21181 agctgcttta tatggcaccc gagacattgg ttcggccaca gatcctggag ttgcttgaag
    21241 cccgtccgcc atccctgatc gccatcgatg aagcgcactg catctccatg tgggggcatg
    21301 acttccgtcc agaataccgg caacttagca tcctccgcga gcgctttccc aagctgccct
    21361 gttttgccct cacggccacg gccattccaa aggtgcggga tgatatctgc gctcagttgt
    21421 ccatacctcc ggagcatcag gtgatcgaaa gcttcaaccg ccccaacctg ctgttggccg
    21481 tggagcccaa gaagaatagc ttcaaacgct tgataggctt tctgtccagg cataatggtg
    21541 aaagcgggat tatctactgc atgacccgca agaaggtgga ggacatagcc aaccgcttgg
    21601 ttttggaggg ttttcgcgcc ttgccatatc acgccgggct cagcgatacg gaacgtcacc
    21661 gcaatcagga agctttcatc aacgacgaaa ttcccataat ggtagccact atcgccttcg
    21721 ggatggggat caacaaatcc aacattcgct ttgtggtcca tctcgacctt cccaaaagcc
    21781 tggagaccta ttatcaggag atcggcagag cgggcaggga cggcctccca tcctcctgtc
    21841 tgctgctgta cagctatgcc gacgtccgaa ccctcaacga gatcatctat acagatgacg
    21901 agaccatgaa tgaaatcacc agacgccatc tggacgcgat gctgcgctac tgcaagcatg
    21961 agggctgccg caggataccc ttgctgcaat ggtttgggga acactataaa gagtccaact
    22021 gccagatgtg cgacaactgc ctggctgata tatctgaaaa gatggatgcc agcgaacagg
    22081 ccaagatgtt tctctcggcc atttacaggg ctcaggaaaa gtatcccgcc aggcgcctaa
    22141 tcaagatcct gagaggttcc aaatcaaagg aattgctgga agcgggcgac acgaaactga
    22201 gcgtgtgggg gattggcaaa cagtggtccg aggtcatgtg gttcagtctc ttccactcgc
    22261 ttaaaaagga cggcagcctc aagcaggaat atcccgattt caacctcaaa ctcacgcccc
    22321 ggggctggga aatcctcaaa agccagcgga gtttcacgat gccggtctcc ctgatggaag
    22381 tgcttttaaa ggacaaggaa gaaaagattg aggatgagct tttcgaccag ctgtcggcat
    22441 tgagggcgga gatcgcttcg cgccagcaag tgcctcccta tgtggtgttt agcgataaaa
    22501 gcctcagaga gatgagcagc ttctttcccc aaagcgcgga gagctttaca aagatcggcg
    22561 gagtggggac cttcaaggcg cagcgctacg gtgacgtatt tatcagactg ataagctctt
    22621 actgccttcc cagggggatt gctgacaaca catcaacgaa aacaagtgtt cccagtaaga
    22681 aagctacaat cggcaggacc gtggaagtgg ttgactatct gaaaggcggc cacaaccttc
    22741 aggagacgat ggtgcagttc ggcatcaaac tggaaacgct gctggaccat ctgcagaggt
    22801 ttctggaagc tgacgggtgg atagatccgg atctgctgag aagctgctgc acgctcggag
    22861 aagcggacgc cgccagggtg aggaactgct tcaggcagct cggcacggaa gctttggggc
    22921 aggtgtaccg tgccctgaac gaagaaatcg gctacacgga cctcaagctg caaaggatga
    22981 tcatggtggc ggagctgaat cccggtgggc aaaaccctcc tcctgccgat aatccttaaa
    23041 tccagactat gccgcaggcg ctgatcgcgc ctcagcaaac ggggacgctt gctgttccat
    23101 tagcggttgc tcatcgcgag aaatcggccc tccggccaaa gttcttgaca taatcggcct
    23161 ttttggacgc tggtaacagc tatagagaaa atccgcataa ccatgaatag aatactcctc
    23221 ctgatcaccc tcctcatgtt gggctgtcag atcctgctgc tcgcgcaggt gtccgacgcg
    23281 ggggagcctg tgtccgtcac cggagcggat tctctgggtc agagcgcttc cgtgatggac
    23341 agcctctttt atgccgcgga cagcgtggcc tattactatt cccaggataa aatcgagctc
    23401 agcggcaatg ccagggtcag ttatcaggat tccgagatca gcgcggacag catgtatatc
    23461 gatctggagc gggagcaggc cttcacccac ggctccacta tcatgaagga cggcgagcag
    23521 atcctgttgg ggcgtgacgt cagctatgac gtggataccc agacgggcct gctgaaagag
    23581 gggatcagcc gcatcgaaaa atcctattac ggcggcgtgg aggtgcgcaa ggtgggcccc
    23641 aaaatctatg acgtggagga agggaccttc accacctgtg aacacgccga gccggatttc
    23701 tggttcagcg ccaaacagct gaggatctat cagggcgaca agatcgtcgg caagcctgtg
    23761 gtggcctggg taaaccactt cccggtgttt tattttccct ttctcacgat gtcgatccgg
    23821 cggggcaggc atcccggttt tttggtgccg gagcctggct acaacagcgt ggacggcaaa
    23881 ttcctgcgcg acatcgcctg gtattatccc atcaaggatt acgccgatct gatcctgggg
    23941 ctggacctca tggagcgcag cggctggaag gccaagctca gcacgctcta cacccaacgc
    24001 tatgagtttg acggcaacct cagcgccgcc tatcagaaac aggtcctggg cggcaacacc
    24061 accttcgact ggagcctgcg ggccaggcat catcacgaac tgggtgaaaa ggccgccttt
    24121 gatatggacc tcgattttat cagcaacaaa cgtgtctggg agagcagcaa ctccctggac
    24181 gaaagtctgg cggaacggct cagcagctca atgtcctatc gccagccgct gctgggttcc
    24241 tatctgaacg taggcgcgct ctacactcag gacctcgtca acgaccgcgc cacggtctcg
    24301 ctgcccagcg ccagtttttc catgccgggg caaccggtct atgagctctt cttgaagccg
    24361 gagcgcagtc caaatacctg gtggagcaac ctcagctaca actatagcgt gcgcctcgat
    24421 cacgtgggcg aaatcaacga tcccaagccc ggaatcatgg atatcctatg gaacaattcc
    24481 atcgatccca tcgattccac cctcatccgc acccgccaca acgcagggat cagacacagc
    24541 ctgggcctca gctacaactt caagctcttc gggtggctca acactcagca gggcctcagc
    24601 tacaatgaag cctggtttga ccgcgacaaa aacgacgaca agtgggtgag gggcaacgac
    24661 tacaacgcca gcgccagcgc caacttcaat atctacggca tccgcaactt ccggagcggc
    24721 tggctgagtt ccatccgcca tatcgtcacc ccttccgcca gcgtcagttt caagccggat
    24781 ttcagctcca acgagcgctt ctacagcttt ggcggaatcg gactggcagg aggcgaggcc
    24841 agcagcaatc tgagcctcaa tctggagcag aaatggcagc tcaaataccg cagcggtaag
    24901 gaagaaaagc gcctgggaga cgtgctgacg atgagcagcc gcgcttcggc cgacctgaag
    24961 tccaaagagc gcaagttcac caatatctca cacagcctgg ctttccggcc cggcagcttc
    25021 aatctggggg ccttcaggat tcccggaacc agcactttcc tcaaagggat cagcgtcaat
    25081 tacggcgctc agttcagtgc cagccacgat ccccgcgaca tcaacttttc cagtctgggg
    25141 ctgcgcaatc agtatttttc ccacagcttt ggcctttccg ggacggcggc atacaagacc
    25201 tggttttcca agcccaaaaa ccagatattc agtccctatg aagccgcgga cagcctgcaa
    25261 acggaagcgg aggacctcgc ctccgggggc tcggacgatg gctggaaact ctccctcagc
    25321 caggatatca atgccgaaaa ggatatcttc gatccccgcg gcagcaacct gagactggat
    25381 gccagcctca aactgagcca gaactggctc ttgagctatt ccaactacta cgacgtcaaa
    25441 aacagcgacc ttatttcgca gagcatttcc atctcccgaa acctgcactg ctggaagctg
    25501 gagatgagct acagccgtcg caacgaattc tgggagtatc ggatagccct cttcaacgtg
    25561 gtgctgccgg acgccctgag gtttcagacc cgtgacagca aacgcaatta gacgggccgt
    25621 gtttctggac cgcgacggct gcatcagtcc ggacgagttt ggctatatat ccgatccctc
    25681 ttcatatcac ctctattcat ataccggaga agcgcttagg atgctcaagg aactgggctt
    25741 cctgctcttt gtggtcacca accagtccgg catcgccaga ggctattttg gcgtagaaga
    25801 gctggaacgg gtgctggacc ggatgcgcgg tctggtgtcc caggaaggcg tcgagctgga
    25861 cggcgtatat tattctccct atttcaaaga cggcatcgtg acgccctgga acgtggagca
    25921 cgaagaccgc aaacccggcc tggggatgtt tcgcacggcg ctcagggagc atcgctttca
    25981 agcctcggcc tcatggatga tcggcgaccg ctataccgat gtggagttcg gcaaaaaagc
    26041 cggcctgcgc accattctat tactatctgg aaacggctgg gatgagttga ggaccaatat
    26101 tttgagctgg gaatatcagc cggacctgat ctgcgaggac ctgctcatcg cggcccgggt
    26161 aatccaaaag ctggcaaaat gaagcagaca tgtttcccgc ggattatgca gattttggcg
    26221 cagattccgc tgattttcgc aaatatgatt agaagtacat gtactcgctt tatttatatc
    26281 tgcgtaatct gcgaataaat ctgcgtaatc tgcgggaaaa actgcgggaa aggtgattga
    26341 atccatgaaa ccgctattcc attatcagga aaagaagttc cgcgctctga gcaagcctgc
    26401 ccagctaaag gctttcaccc gcctgatcag agagctggaa cgccaatcct gccagactga
    26461 tcccgaaccg aaattgctgg aaatgatcgc caaaacggct ggctggatag aaacggaaga
    26521 cccactgctg aaaagcttta tggacctggc aagctggggg accgataaac gtgaaacagc
    26581 cctcaaagca gccgcctggc tcatatccca agaggcagtt cccagagacg acgctatccc
    26641 ggtgcgtcaa aacgatggcc tgcgcgtttc cgatcctcac tttgtggaaa gggcccaaaa
    26701 tatggcggtg atcctgtcag acctgcgttc cgctttcaac gtgggctcca tcttcaggac
    26761 ggctgagtgc ctggggatag gggagttatg gctctgcggc atcacagcca gacccggaga
    26821 agccgctttg gaaaaaaccg ccatgggaac cactgacaaa gtggcctggc gaggctttgc
    26881 cactgccatc gaggcggtcc aaaccgccag aaagcaaggc agaatcgttt acgccctgga
    26941 gacttccggg caggctgtaa gcgtgtttga tgcctcaatc agttttccct gcgcgcttgt
    27001 ggtgggcaac gaggccctgg ggatttctcc agaggtcctg aaggcttcgg atgaactgct
    27061 ggagttgcct gtcatgggct ggaaaaactc gttgaacgtg ggcgtggcct ttgccgtctg
    27121 cgcctatcag atgatcttca aaggagccaa atgatggagc acctggattt ttatagaaag
    27181 atatgtgact gcctggcaga gaattgccgc ctctggcagt tgagcgttgt gagctgtgaa
    27241 ggctcctctc cggccaagcc tggcatgaag ctctgcgttc ctctgcaggg cagggaattt
    27301 ggcaatctgg gcggcgggga agtggaacac agcgccatcg cctgggtgag ggaaaaccgt
    27361 ccttccgaac ccgtattgag gacttatatg ctatcggaac agggagatca ggccctggca
    27421 gatgaagaaa ttcccacgcg gatgatctgt gggggcaaag ttagcatcct gatcgagcct
    27481 ctggccaaga gcagaaccct ctacatcgtg ggggccggac actgcggcag ggctttgggc
    27541 cagcttgcca aactcgcggg ctggtgggtg catctgatcg acaaccgcaa tgaactgttg
    27601 gaaagtgatt tatctctata tggagatgta gccagccact cagattactc agacgtctcc
    27661 accgccatca gctttgacag agaggcccac atcgtgatca tgacccacgg tcacgcgcac
    27721 gacaaacagg tgctggagca atgcctcaga cgggaatttg cctatctggg catgatcggc
    27781 agcgccagca aagtggccca gactttttcc aggctgaaag agcagggttt ttctgacgcg
    27841 gaactggcca gagttcacgc gccgattggc ctcccaatcg gctcccatac tccttttgaa
    27901 atcgcggtgt ccatcatggc tgagctgatc tcaatccata agcggaagta aacgcaggag
    27961 agccgccaag gtcaccgttt ggtctgagtg ccatcgattc ttctattcca gccgctttca
    28021 ctcctggcac ccggtcaagt caaatctgcc ttcccttcag cctcctttgc atcacccatg
    28081 cccctcctgt gcctctccca tgcctaggca caggaggggc atgggtgatg caaaggaggc
    28141 tgaagggaag ttgagggggt tgaggtttag tgagtttggg gggcttggat aactgaaacg
    28201 gctgaggaga ttggaggtta gtgaacgtag tttcgataga tccagggcgc ggttaggctt
    28261 gtaagccctg tgaaaaagca aaccttgttc tatttggagc cttatcgatt ctcatcaatt
    28321 catcaattcc cagtcttagc acccacttac cagagccata aatgagaacc ccgggctatc
    28381 acactcgggg ttctcatatt caatgttgag gggttagctc tttcaggccc tgggtatccg
    28441 ctccgcgatc tcagcactga gcgccgcgat ttcgccttcc agtttgccgc ccatctgacg
    28501 ggctatgcca cctgggatga ttttcgcgcc ccgggtggcg gcttctctgg cgatggcacg
    28561 cagggcaccg ttacctccca gacagcggaa ggggaagccc atggtggcga aagggaccac
    28621 cgttttgccg ctgagtttac cgagctgttt gacggccgcg ataattaccg gcgagggacc
    28681 aaaagcccag acgggtccgc ccagcatcag caggtcgcaa tccgagatat ccggcagatt
    28741 ggtaaagcgg atatcctgag gggcgcggac gctgccttcc ttgacagggc tgactgtggc
    28801 cagaggggtg atattgacgc tgtgaccggc ttccatcagc cgggacgcga tggcgtccgc
    28861 gaagcgggtg gtgttgccgg tcttggagtg gacgaggatg tgaatcttca ctttagcatc
    28921 tccgcggtcg gctgggcgtt atcgttgcgt tcccagcgat cgtctatttt ggtcttgacg
    28981 gggctgtttt tgcggaaata gttcacaatg gcgtcgcgca ggttgatctg atggttggtg
    29041 atgtcctcct ggggcacctg gaccagcagg ttgagtccag cgttgccctg catcagaaag
    29101 tccgtcgtaa cgcaagtgta gatggcgttt ttgtcccagg gtttaccggc gattttaagg
    29161 gaagtgacgc ggtcgaaatt ggggcgtttt ttggaataga ttacattgac accagagacc
    29221 accagtccgt gtcggccgcc ttccacacgg gtctcgatga ttttgcggag gaattctccc
    29281 gtgcatttga acgataccac catgttgtcg aagggcatca cgtcaaagat actgcgatag
    29341 gtgacggggc cacttttgat gtcggcgcgc acccctccca gattgaggaa ggcaaaatcg
    29401 gcgttgacct cgtggcgcat ggcatccgtg atggtgtttc ccatcaggct ttgggcatcc
    29461 acgttggtgc gggagagata gactgctgag gatcctacga tttcatccat gccctgttcg
    29521 gcgatggcga cctgctccgc gatggtgtgt ccgatctcgg gatcggggat aaactgatcc
    29581 tcaaagagag tgatcatgct gccttcgcgg atggcggggc tttcatagcc tgtgagagtg
    29641 cgggtctcgg gatcgatggt gagggtgatc cagccgaggt tggagccgta ggcatagccc
    29701 tgaatgacca tggtgtgggt gtagggatcg atccagggtt tgggcacgcc gcggtgcatg
    29761 tggccgccga taaagaggtc gatgcctttc acttcgcggg cgatctcctg ggcgtcatag
    29821 ccccagacgg cataacgttt gtccgagagg gggttgccct tggcgtcata gcggctgaga
    29881 tagccgtctt cgatgtcata aggcaggccg gcgtggccga gaacgatcac gatatccacc
    29941 ttctctttgg cacggaccac attcacccat tcctgcacgg cttctttttc gctgatgaaa
    30001 tcgatgtttt tgatgttttc agggaagctc atcttctcgg tatcggtggt ggtgaagccc
    30061 acgatgccga tgcggacgcc catgcggctg atcacggtgt agggctttac ataccaggga
    30121 aattccccgg tgcggcgatc cacgatgttg cagctcagaa tgggaaaatc ggcgaggcgc
    30181 agagtttcca tcagctcggt atccatgatg tcgtattcat ggttgccgac ggtcatggcg
    30241 tcataaccga tggcgttcat gtattccatc acggcctggc ctttggtaac ggtgccgacg
    30301 gggcgaccct gaaagaaatc acccgcgtcc aggaggatgc tttcccgctg tttggactgc
    30361 agagaccgca catgtttgat gagagtggcc gcggaacctc ctccacccag ttggggaggg
    30421 aattccgggt tcatgaaggt agcctgggca cgatcgatgc cgccgtggac gtcattggaa
    30481 aacatgatgt ccagccgcag atcttccgcc cgggcgggat gccaggcccc cagcagcagg
    30541 acggcaagca ttcccgcgag gagggcaagg gagtggttat gtctcatagg tatttaccag
    30601 ttaaatgtta atccagcgtt gaggctgatg ttgttttctc tcactttgtc cggattttcc
    30661 cagccgcgat ccgccagagt gatgtgggaa tttggccaga gggcgtaagt gctgtttccg
    30721 ctgtaggttt tgtcgttgta atagctgtcc gcaaagaggt ccagggcttc aaattcacgc
    30781 caggtgtagc cgaagccgag gtccatctgg aggaagctgg tgagcgcgaa gctggtgccg
    30841 gcggagattt tggggctgat gatgcgtttg gcgatgatgc tgccgtctga ttccagttcg
    30901 cgcagatagc cgttgacggc ctggaaaccg aggcgaaggg gcaggcggtt ttccacccag
    30961 tgttccactc cggcgtagag gtttacctgg tcttcaaagt gctcgctcac atcgctccac
    31021 ttcacatatt ccacatcgag gttaaaccag gtgcgcatga tgttgcgggg ctgatagagg
    31081 aatcccagcc gcgcttcaga gggcaggata tagtcttcag cgatgggagt ggaaatatcg
    31141 atgccggcat tctgataatg ggcatcctga agctcatggg tgctgccggt gcggtccaga
    31201 gtggatttca tcgtgtaggt ggcggcgagg ccaaagcggt tgttgagccg catggacagt
    31261 ccacccttca tttgggtgcc ttccagttcg gtctcggtgc tgagggtgta atcgggcagc
    31321 accttggcgg ggttgtccac cgcgtccgta aaggtgtcga tcgcccactg ggaccagcgg
    31381 atggtcttca tctgggtctg ttctccgctc agcagggata cttccgcgcc gaggttgagc
    31441 tccagatttt cacccagttc gtaaccgaaa ctgaagacgc cggcggcctg attgaggctg
    31501 ccttcgtttt cgatggcgtt gatggcgatt ttctcgggat agacgtcgtt gtccgtgttg
    31561 cggttgttgc ggatctcttc gatgtagttg ccgtcgaagt tgagcagggg cttgtaatag
    31621 ccgcccaggc caagcttgag gaggccaaag cgcatggaag caaatccaga accggcatag
    31681 tcgtcataga agttgatatt ggaggcatag acggcgtcgt ctatatagtt gtcgaaggag
    31741 ttgtagagcg ggatatagcg gttgtcttcg gcacggttga ccagcagagc gccctggaag
    31801 cccacccggg atttgatcag agtgagattg gcagggttgg cggcgatgcc gaagggacgc
    31861 aggtcgttga aggtccccgc tccgcccatg gcaaaggcgc gggcttcaaa gccgtcatat
    31921 ttgttgccga aataggaatc cgtgatggac caggcggcaa ggctgggcgc ggccaggatc
    31981 atgacggcca gcatcagtat tgtcatgccc gcgggggcgg aaatccattt attcattcta
    32041 tcttgattca tttattcctc cagcttagaa gcggtagtca agggagagac ggatggtgtt
    32101 ttctttgtgc tccacgcgct gatagtagtt ttcgccctcg acgggaacgt tgtattcgcg
    32161 gatggagtat tctttggtct gataggtctt gttgcggaat ttgaggctga gatacatgtt
    32221 ttcggagacg cggctgtgga gggcgaccca ggctttggca cctttttcgc ccataaagtc
    32281 gatctccata tcctcccagt cccagtggct gatgccgttg ctgaaccaat agaggaagga
    32341 gccttgcagc ttcaggtttt tgttgaagtt gtgggtgtag ttcacgccga tatagtctcc
    32401 ggtcataagg gtcatggcgg cggccatgga gttttcaccg ggattggcgg gattggtgag
    32461 cgaggtgtag ggagggctga gcacggtgtt gtagcggtac tcaaactcca ggaagtcgcg
    32521 gttggagagg aaggtgcgaa tgctggcggt gtattcgttg gtcttggaga caccgcgctc
    32581 ggcctggtcc tcgatgcggt tcacctggtt cttgtagcgg agacggagtc ccagttggta
    32641 gaggggacgg aattccagct cgctctggaa cctggcagtg cggcgtccgt ccgtctttct
    32701 ttcaaagatg tccagatagc tgcggccgac ggtgaagtag ttgttaaatt tatagcgggt
    32761 ctcaaaatag actccccgct ccggctgggc ctgagtggag ccctgataga cgtctgccag
    32821 gatggggttg gtgagggtat agacgttttt ctcgatgatg gtgtcgtcaa agcgctgatg
    32881 ctctgaaaag gcgttgctgt aggggttgtc gaagccgacg tcgtaatccc ggtagagggt
    32941 gatgaaatag aggttttcaa actgggtgta agcgctcagc agcagcgcgg aggggtcgtc
    33001 tcccagcttg tggtctttac cggtgacggt gagctcggca tattcaccct ggatggaagt
    33061 attgccgatc acggtgccgc cgtcgaagcc gatcactctg cgaaaattgc ggtcatagac
    33121 tccgggcttg taggtgctgt agagaccggc aatctcggca tccatctgtt tgaacttggg
    33181 atagtaatag gaatcgcgca ccagcagagt cctgagatcg tcataatccg gcaccatgaa
    33241 atgggctttt tcgtagagcg cggtgtaggt ggtgaagccg agacgggtgc cgatcagcgg
    33301 gctcacctgc acgtgggtgc cccagagctt ttcgcgcagg gcgtccttgc ggaaggcgag
    33361 attgacgtag ggcaccgcgt aggccgttcc ggactggagt tcggcgttcc aataggcttc
    33421 cgcttccctg agctcgtcat tgtcaaaacg ggcggaggga accacatagc tgaagacctt
    33481 gtccttgccg ttttctttga cggtgttgcc gtcttcatcc acatacatga tggcgtcttt
    33541 atcgtcctga gaggcccaga cagccacgtt gaccatggga ttgttgattt ccacggcggc
    33601 cccgcgcagg gcgaattcct gggtgcggct caaatccgga gagatgccca tgatgcgttt
    33661 gctgaagcca aaaccggtct tgcgggcgct gtagaaatcg gtgttttcca tcaccaatcc
    33721 ctcgccgaaa gtggcgcggt agtggcccag atatagcttc aggcgggtgt ttcccagcca
    33781 gggaagctgg ctgttttcat agccggcata aaacttggaa tcgttggcaa tatcggtggc
    33841 gttgcggtcc aggaggctgg attcgccctt tccggtgtag ttcatcagac cgatcttata
    33901 gttgttgccg taacgggccc gcagcttgag cacgatatcc gggtccagct cgtcgaggtt
    33961 gaaatacccc caataggcga ggtctttctt gtgctcgatg gtcaccgccg aattgccgta
    34021 atcgctgcgc aggaaggact cgtgatacat ttcataagag ccttcctcaa aatagcgggt
    34081 gaaaaactgc atctgcgcgt catagagcat gcggtttttc accggaggct ctctgtaata
    34141 gacgtagctg cggaggttgg tgtagccgta gtgggagagt cccacgctgt tgcggaggtc
    34201 tcgcatgtcg gcgatcgtgt cgcctctggc tacccggttt agaatggcaa cggcatcgat
    34261 ggccgagacg ttgggcaggc tgaccaggtc gtcaaaatgc atgcggttaa cgttttgggg
    34321 ggtcatcaga tagtcctccc agacatccgc catgccttcg gaagcgcctt cgttggaatc
    34381 cagccgttcc agcagatcgc ggatttcctc gcggcgggct tccacatcgt cagtttcctg
    34441 atagagggag accaccacct ggtctttgat ccgcagcaga gtgcgctggt ctatggatgg
    34501 aatctggcgc agatcaaaaa ggctggagaa aatggacacg tattcccggt attccaggat
    34561 gtcgcgcgcc tgtttctccg tcagatcgag ctgtctcagt tcgctgtaac tggccgtgtt
    34621 aaggtccacc ttggcggcca gcagccccag gctcatcagg gccaggagca agatcagaag
    34681 ttttctcatc gcttttcctt gcagtacttt atcattttat taagatcgca ttttagcacg
    34741 aatagacagc aaatccagct acccaaatct gtaaagcctt tttttggggg ctggggtttt
    34801 gagtgtgacc agggagatac a
//
LOCUS       DAOSFN010000008        34258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_994_length_34258_cov_8.806830, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000008 DAOSFN010000000
VERSION     DAOSFN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 34258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34258
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_34258_cov_8.806830"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1345
                     /locus_tag="PLO35_01285"
     CDS             167..1345
                     /locus_tag="PLO35_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOH78324.1"
                     /translation="MKPLLRLALKRIDYQDALKASGVSVQGGVMIHAASLGEVNAVSS
                     LIREISHHLAPERILITTTTVAGRDAASRLHPDIQVRLSVLDVASLRKKQLEIFAPGL
                     ICIVETEIWPNLLYQAGQRNIPILFLNARLGQKSLQRLKKWKPLLQRVSGSVKAILAK
                     SEDDAIRFKELFPAPVQVAGNLKFALKPPVFDPLEVKRELGYTDEDLIICVGSSRPGE
                     ENLIMRAFFELRNQHPNLKLIIAPRHMQRVEEVKRFCGATGFHLYSQLNGTPASDPVL
                     IIDQLGYLNKAYSICDIAIVGGSLFDFGGHNPLEPAFYSKAILIGPYHDSCRESVHEL
                     AKADAIVVTERQHLNKRLATLLDDPSERHAMGTRAKQVITRFQAPMDAHLNEILKWIN
                     "
     gene            1333..2352
                     /gene="amrS"
                     /locus_tag="PLO35_01290"
     CDS             1333..2352
                     /gene="amrS"
                     /locus_tag="PLO35_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOH78325.1"
                     /translation="MDQLTREAMYWTPLDDVADGTVRCDLCPHHCIIEVGKRGLCRSR
                     FNRAGKLYAQNYGQAISLSIDPIEKKPLYHYYPGSQILSTGPNSCNLSCFFCQNYTVS
                     QQECPSTWLSFEALEEYFELHPKLPRRLAITYTEPITWYEYIMDLSERLPDLRIVLIT
                     NGYIEAEPLEALLPRIDAMNIDLKSIREDFYRQACSGSLAPVLHTISRADHEGVHVEI
                     TNLLIPGLNDSEEDIVQLAKTIARINWEIPLHFSAYHPAYKSSIPATNPESVLKARDL
                     ALEHLRWVYAGNIGPGEFCDTYCDKCLEHLISRKPGHSWCDVAFDDYPICPTCLEPIW
                     GRFEV"
     gene            2282..2710
                     /locus_tag="PLO35_01295"
     CDS             2282..2710
                     /locus_tag="PLO35_01295"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOH78326.1"
                     /translation="MWLSTIIRSVPPVWNPSGVALRFKHSVKQLTAADLVLILGVVTA
                     AVLSAIWLWKSDSQKTVFIYKNDQLWGEYPLDKDQVIRIDSHNTVAISKGRVAMTHAD
                     CPDKRCVKQGFSKTLPIICLPNRVLIEILDREEEAPHILY"
     gene            2716..>3929
                     /locus_tag="PLO35_01300"
     CDS             2716..>3929
                     /locus_tag="PLO35_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78327.1"
                     /translation="MLKIAIYPSDHGFGHAARMGALAQEFIALGMQVRIRSTRPDYLF
                     SCLESPFFCKDDVRNDVGVLHTAGLKADLGNTKASLIKALSFRHAIVEREVNWLRREE
                     ISLVIVDVPYLIIEACAYAGIPVIAVTNFDWVFIYARLFKRDIEMRPVINTIHSLYQR
                     CDAAFRLPFSSSASMAAFRSPMKSGLLARRKDRYCDLRELYGIEQDKPILTCTFGGEP
                     GMSVDLKSVCAAWPGMVISASEEVSAANHIHAPRHADWLDLIHGSNVILTKPGYSTFA
                     EATQFNRGIIYLPRQDYPEEEVLIRGLKLYLPKLEVSQAPKGVKAWQQIFRSLPSPQK
                     PHPAFRNQNSRVAADIIGSYLKLRQSSLTSIFDIGSNNLNYLLWDAPNHRILHTAHFT
                     TRLAKLSNPVAV"
     assembly_gap    3930..4029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4030..4398
                     /locus_tag="PLO35_01305"
     CDS             <4030..4398
                     /locus_tag="PLO35_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78328.1"
                     /translation="LSDSQQRASLMSILPLIHNAQDMQLVLTGLTGTFLAAIVNKKSN
                     ALPWEFHNTRLSRRQIDQLAAAVAGKVSEDWEPYLQTDTAISILEASLGLVGIIMDKY
                     EAAEIVVCYYGISLGQALKQ"
     gene            4432..5718
                     /gene="miaB"
                     /locus_tag="PLO35_01310"
     CDS             4432..5718
                     /gene="miaB"
                     /locus_tag="PLO35_01310"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOH78329.1"
                     /translation="MRFFLETYGCQMNLADSELVSSILIEAGHLPVSNIDSAEVIIFN
                     TCSVRQHAEQRVLGRIANEKHRKLQNPGLRIGVIGCMAQRMGKRLMEIEPGVDFVVGV
                     DQYGMLGSILNDSELPDDDLISMDSSQIYSSILPERENQHCAFVTIMRGCDNFCTYCI
                     VPYVRGRERSRPMGDIYHEVKDATLKGYRDITLLGQNVNSYSHDGYDFPGLLRRLNEI
                     DSLWRLRFVTSHPKDLSDSLIRTMADCDKVCEHIHLPLQSGDDEVLKRMNRSYSSAHY
                     LDIISKLRKAIPDIAITTDLMTGFPGETDLQYENTLRLMREIEFDYAFCFKYSCREGT
                     EAAAYDQQVPESLRLERLQQMIDLQREITLRKFQAKIGAQVEVYVENLSRKSADQLSG
                     KTRDFKIAVLSGKADQIGKLVSATVKTATAGTLICE"
     gene            5834..6742
                     /locus_tag="PLO35_01315"
     CDS             5834..6742
                     /locus_tag="PLO35_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HOH78330.1"
                     /translation="MEDKDQGVNMKSMTGYGKAKYSANDIELEVEIKTINGRYLDMKI
                     ISPRELSFFDHSLRRKITHSLHRGTVEIRVTFSDHREPSVRLDTTKLRKYHEVVKSAQ
                     NYLGLDEPLPLQFLLSEPGVIEIGNSLDEDPLLIEALETTVGRAIDAIIASTAEEGAQ
                     IKAVIMDSLARMKQALDEIEKEIAPFKQELLEKLRNRITELLSNVNLDNLEQRLVQET
                     AIYVDKYDIQEEISRLRSHITTFERTLAKDDEPEIGKTLSFITQEMHREANTLGSKFS
                     TTKTFVHVLILKEEVEKCREICLNVT"
     gene            6732..7169
                     /locus_tag="PLO35_01320"
     CDS             6732..7169
                     /locus_tag="PLO35_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78331.1"
                     /translation="MSREILLKWRSWPFVERPKTSIALVLFLLMLAAILWHITVTTWK
                     APLFYYIGMILVLTNLLPWFIPTEYELDHSEVRVRYLIITIKRNYSDFGCFYQDKRGI
                     MLSTFKMPRRLDPFRGQSLRFSGSRAEVEALNAILLDKIGKKY"
     gene            7295..9481
                     /locus_tag="PLO35_01325"
     CDS             7295..9481
                     /locus_tag="PLO35_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOH78332.1"
                     /translation="MKKALEELSAAGKLVYVKRRYSPPLPKKQHRTERVPPAPMLSSE
                     LIEGVFDATPLSRNFSYAFVRTEKGDFFVGSEDTLNAYHNDKVAIEPHFRNGKADYGR
                     VRRIVTRANERMAGDLSFSSGKAIFICSNPKIHNWFEITDTNGAKEGEKVILQVSNWG
                     SPLAGKMPGGKVVEVLGPSGDPQVELIAVIRQYDLPLSFCDEVMEEVNNLPDSIDARE
                     VARREDLRDIFTFTIDPASAKDFDDAISIRKDSNGYTLWVHIADVAHYVKPETRTFDE
                     AVKRGNSFYFPKKVIPMLPERLSNLICSLRPDEDKLCMTVITHFDPKGRTTAQKMVES
                     VIRSNHRLSYEQVDELFETGKSDFSKDLKDALSWSRELSALLSAKRVAAGYIFFDLPE
                     IEYEYDQEGFVRRLNLAEETESHKLIENFMLVANEYTAEKLSQLSPTSIYRVHENPDP
                     RKLERLGESLSYYGLEYIISEDVNKSLQYLLNSMPNADYHKVFDRMVLRSLKKAKYTP
                     EHLRHFGLGMENYTHFTSPIRRLCDLVVHLLCKTYVIKSSKLKLERKQIALYAEIASS
                     QELLADEAERDIDRTYSLAFMKKHLNERFHGMVIGTNSSSLIVRLTEIPITAVLKTAM
                     LPGGPWAYQDRAMRFVNTKTNYYYQLMDKLIVDIVDVSDDIYLELANIDSAHTHAQVL
                     SPQPQTKIPKTSPGRSSGPRGRNRIDVMTPSPKSGRHSGKKRGSRR"
     gene            9481..10284
                     /gene="murI"
                     /locus_tag="PLO35_01330"
     CDS             9481..10284
                     /gene="murI"
                     /locus_tag="PLO35_01330"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOH78333.1"
                     /translation="MKRPIGIFDSGVGGLTVYKAIRNNFPEEDLIYFGDTARVPYGPK
                     SENTIIDYSIQNARFLLQRGIKILIVACNTSSAVAIPVLRKMAQIPVIGVIDPGAQQA
                     VTATRNRKIGVIGTEGTVRSGAYTKALQAIIPEVEVFSVPCPLFVSLAEEGWQSHPVT
                     EAIVREYLSYFSDKDIDTLVLGCTHYPLLADVIQAVMGPKIKLVDSAAAIAAYLEPLL
                     PTENDGQPGQDSFFVSDNENKFAALATRILGSEITQLKRVRMFESWFED"
     gene            10315..11142
                     /locus_tag="PLO35_01335"
     CDS             10315..11142
                     /locus_tag="PLO35_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOH78334.1"
                     /translation="MEPLILTAAITGAETTRKDQPNLPITPSEQAAEAAACFEAGARV
                     IHLHVREDDGSPTQRLARFEESILAIRDAVPEVIIQISTGGAVGESFEKRLAPLALKP
                     EMGTLNAGTLNFGDDIFINHPKDIIRLAEAFKLYDVVPEVEVYESGMIDVIARLVKKE
                     IITHTPLHVQFVLGVPGGMSGKPANVLYMASHLAEEIPTATWAVAGIGRWHIPASMMA
                     MTLGGHIRVGFEDNIYYHKNLVADSNAQLVARMARIATEIGRPLASPAQAREILGLK"
     gene            11150..13390
                     /locus_tag="PLO35_01340"
     CDS             11150..13390
                     /locus_tag="PLO35_01340"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOH78335.1"
                     /translation="MQLSDNALIVLEKRYFKKDGEGRILEDWDALITRVATNVAGNDP
                     DKIMVYKELLDSGNFLPNSPTLMNAGSDLQQLSACFVLPIDDSMDSIFETLKNAALIH
                     KSGGGTGFSFSRLREANSRVRSTNGVSSGPLSFLKVFNAATDAVKQGGTRRGANMAIL
                     NVNHPQILEFITCKADTKELTNFNLSVGITEEFMRAVYQDQEYELISPHTGEAQRSQN
                     AREVFELIVEMAHKNGEPGIVFLDRINADNPTPALGQIESTNPCGEQPLLPNEACNLG
                     SINLSLFASGDTVDWAKLKAVVRLSVDFLDDVIEVSHFPLPQIDSMVKANRKIGLGVM
                     GWSDLLYQLKLPYASEEAVALASRVMEFIDFSAKQRSMELARDKGSFPNFPDSSYANG
                     INNRKQIDQDWEGLSKAIAINGIRNATVSTIAPTGTISMILDTSSGIEPQFSLAYVKN
                     VMDGEKLLYVNKWFEKALDREGLLRQDILERVSQGESLHDLEEIPQAIRDVFQTAHDI
                     SPEWHIRMQAAFQAYTDNAVSKTINFPHDATREDIHSAYEMAYQLGCKGVTVYRDGSR
                     ENQVLSTGSTPKTDKKVAPRTRPEVTHGITQRLETGCGHMYVTINTDEQGACEIFVQM
                     GKVGGCASAQLEAIARLSSLALRSNIRLEAIIRQLKGIRCQSPMWHKGQMITSCGDAV
                     GRALEGFLTLHKNGDVKSLQIETDAGLMQIPVKSSGSALCPDCGSTIEHSEGCLICPS
                     CGWSKC"
     assembly_gap    13583..13682
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13721..14749
                     /locus_tag="PLO35_01345"
     CDS             13721..14749
                     /locus_tag="PLO35_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOH78336.1"
                     /translation="MSRKEIISIVILVLVAGYGAWQYFHSSFTQTKSQHLMDTVVRVT
                     ATSKSKNIGTRIDSVFSYIKRLEDKLNDYSEDSWLWKINHSDQTQFAMDPDIYELLVI
                     SDSLYKITDGGFDPTIKPVYDLWGFGSRPYSDTDSLANAVPDSLVLAKTLEQVGFDKI
                     RFDARTLYKPVEVKLAFGAIAKGYILDKARAYMKSMGIISGNIDCRSSMTFYGSSLPQ
                     QVYIQHPMLSQDDYIASFKIYNKSVGTSGNYQQFFELNGIRYHHILNPKTGYPVPNVY
                     SCTVIHPSAAWADGLSTALFLVPPDRAIELARSIPDCDAVIYFDQDGSTVSLKTMGMK
                     DIGFSEKI"
     gene            14746..15558
                     /gene="folE2"
                     /locus_tag="PLO35_01350"
     CDS             14746..15558
                     /gene="folE2"
                     /locus_tag="PLO35_01350"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOH78337.1"
                     /translation="MTIPDLQSQTDHRRINIDKVGVKSLRYPILVEDRENTAQHTVAD
                     LNIYVELPHHRRGTHMSRFVEILNHYHQDMIIGQLDRFLDELKTVMKAEAAYVDISFP
                     YFIRKTAPVSKISSLMCYDCFFNACMRDEYELWIGVVVPVTTLCPCSKEISQYGAHNQ
                     RSHITIKLCYREFVWLEELIALAESVASCEIFPLLKRPDEKYVTERAYNNPRFVEDIV
                     RELTQGLKADPRVKKFMVEADSFESIHAHNAYAKVVWDTETDLPARPKTRKS"
     gene            15558..>16368
                     /locus_tag="PLO35_01355"
     CDS             15558..>16368
                     /locus_tag="PLO35_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOH78338.1"
                     /translation="MPGLHIQPAESFVLPNGLKVILHKDPSEPIVCLQAVVRSGSANE
                     RAPEAGFSHFIEHLAFKSSKRYGFNQISSTVSRLGGSLNAYTDFDSTCYYLLLPSEHV
                     HSGLEIISELLSGAKFTAVDVTTEKEIILEEIGQYSDDPETDFLEFIQARYYKRCPLR
                     KPIVGTPKDVRRATHASMKAFYKRRYHPSNCFLVISGAVDPATLKASVDHLFGAWKTP
                     SRATRIPASEWLEPEFGRSGLHWQKANQELFALVLPELSELHPMADALLIAI"
     assembly_gap    16369..16468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16469..18349
                     /locus_tag="PLO35_01360"
     CDS             <16469..18349
                     /locus_tag="PLO35_01360"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH78339.1"
                     /translation="LPELSELHPMADALLIAIRYLAIGRASVLHKILVEEKKLCSAVR
                     VSSLCGVMSGASAVTFVPSRKMAIPAIARVIRQEYNKLWHLGVPQAEFELIRKDVVHG
                     WLFGFEARENIANLLAAEELLGDYHSLYEYGDRIDKLSPGDVSASLRKYWHPIRLALA
                     YRGPKQLVLPSDLIPSHPDYLHFPKKDLGTKRPSGEISFPALSNQATKPSPELVRVDE
                     RHWQGMLPNGIRFVFKKLAQGPIGSFAVTTPASQLWEGPSERGRNYFLSTMMLYQSGN
                     LSHQAIMDSSRWLGLNIRVSHNVDTTTFKGKCFVDDLAHTLDLTAELLNNPKLDQAYL
                     YTLKAAASDALRRERDYPPSYGYLLWLRAVFGKKNALERSTGSLTDIRALRLSDLNDW
                     YDRYYHAANYSLAICAPLEAQEVWDQVNRSFGRIHFQEHPSRGTTHPIYFKNPRQIVS
                     SGANGQCVIHIGAPATTSKDKVHTTASHMLSQIIGGDINSRFFDLIREQRGLAYQTGM
                     DVVTLDPLGYWTAYAFCGKEDQQRCLDLMKVIIQEVAEHGITAEELELARNYIIGMHR
                     FDSESVSYQASALANLLALGYPLSHLLERNQRIASLDLDTMNLVAARWLRSQDQYIHI
                     LQ"
     gene            18346..19116
                     /locus_tag="PLO35_01365"
     CDS             18346..19116
                     /locus_tag="PLO35_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOH78340.1"
                     /translation="MICLGLDIGSRNTKLVIFDQSAQSIVFSAWQATDIDPLISVSSL
                     LDKALSATDMRLDDFAGRAVTGYGRKLWKSADRVLSEISCHAAGMHYHYPDVRTIIDV
                     GGQDSKIICLSNAAKVLDFSMNDKCAAGTGRFLEMTAIRLGVSVGQLAGLASQSSSEL
                     ALNSTCVVFAESEIIGLMAAHASAANIARAVHRSVAKRVFSQLAALDHHPPYAFTGGV
                     AQNADLAFCLQNELQVHLAIAPDPEITGALGAAILAVK"
     gene            19113..19880
                     /locus_tag="PLO35_01370"
     CDS             19113..19880
                     /locus_tag="PLO35_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOH78341.1"
                     /translation="MILTDYHLHTEYSWDSHLKAENLIAKAISLKYDAIAITEHLDLL
                     PWELGENGLVSLRKYRDRLSELQASYPRLQVLGGIEIGDYQSVRDFASDLISEYDFDL
                     ILGAVHFLVDRTNVAIPLPHPLDKSQIEAYYRLNLRLVNDCDIDILAHLGVYQRYYKA
                     VPEESFALPIIKDIFRTLIERGIALEINYSAYRKPLGCCLPNPAVLDLYLEMGGRLFS
                     IGSDSHHLDHFHDWRHLLPGWTRELVFTHKRLLALPS"
     gene            20258..25885
                     /locus_tag="PLO35_01375"
     CDS             20258..25885
                     /locus_tag="PLO35_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH78342.1"
                     /translation="MYIDHRYEVLESLGSGTWAKVFKVKDIRSGHFYTLKLFQYLSSE
                     DLYARFSAEEMHHITRIEHPNLSHVVDFGHVGDHIYFISDWFDGTPLTAFRFSKGKID
                     LLYDLVAQCAYALHALHTQNILHKDLKPENIIYRQTGKALEVKLIDYGFAVHEQTRDN
                     QLVSGTLPYVAPEVYLGQRTGPESDFYAMGVILYRLCTGTFPFNLDQISALMSGSQQY
                     FIPIFPSELNKDIPLALEKLILRLLERNPDHRFSDAIELIVYLNRVSGRSYPFSLEWS
                     LVNTLRFNSYIVRERYSHQLLDYLPAVRAGNGKIVSLIGGDGLGKDSILSLFRYHLLG
                     GECWLFDYSCTRTDHEAFFALIKEYLRSLPEDEQQKFPSLSRISDKFKAYLNGPEQAA
                     KSYSQGQEELRQDFSAARDLLMELSTRKPIIFIIRDFQYVHRHTVDFINYLSPQLVRH
                     RVLVVLSCNEFNKVRQIENTVLNHIPMLDMDEARDYMARLLSTPVPEDLVRAVYHRSA
                     GNPHFIREILVDLTLRKEIGHPKEERDNKIENGESSDKTRVNQPGTLSVPTGRSYHFP
                     ASLAGYQLPSRVLHSIYSRMSHITELNYARLQKLAIARVPLSRELIIYILKLSDQELY
                     SLINEATYNEILVKHGKLYYYTFPEARQRFFDENSRRAHGLVSRRVLKYYASRSIEDT
                     ETCLGLIENAILAGDALSARRYYLRLYHLQNDDFEQEEAYEAIINVLKIDIGRFEGLV
                     HTGKANGEADCSVVSEVSVPPADIIRDLREFHEKTEATGFFRRAGFIVDNASAIPETS
                     EKYLVLGTLKMLAEELVEARALFQAALDLSMTGRQRVLSYLYLSRVASRVDHNLAKSY
                     LDQIDPADLNLELKIIYTDRLAVYYAAKKEPGMAIRTMESFLESLPPDHDTRAMINLA
                     ALHNDLGVFYSDMKNITEAEEHLNIALNIWKRHNIKRYLGLIYNNLADLCLKQGLTVK
                     AQELSELGYSYSAELNLTMGQALALLNQGEAFIKMGEFGTAEQKLSESLHLVQSIGST
                     QYLVSIQRNLALAKSKIKGFGYFYRFISDIEPQLMQGQIIEVNPLVKTYFYYLHETAN
                     LKKLRRLIRKNVQINYKHIHEEEFYHNILSLIALSGGDYALALSELRQAMHFAGEINN
                     NYAIAVFNVLQVRCLYGLNDLAKARELAETALITIRANSYRYWEINLQILGLKLDLMN
                     PETPLRGILRKADRVLASCREFQYYQLEVEAWQVKLQIISELGIESKAASEFEQYRRL
                     LDLITADIDPEDRENYLHINHYDQKNLKKFAALPLASRRKDLRYRWNDLLYNIANVNS
                     IDRVKFLIEKGLDQVICPARFKLMVFSDKISNFYCFHCFNCGRDTMVPPELGPLIEKA
                     FGQDSIVSCEYKGQNTQIIPLVSGSKRIGYLLMSDDGELEFTRNELTLLKNIKQHLTA
                     LIVRVNDYSQITHRVSKMNELMAISNELMGIVEINDLQREIVSTAIDFTNATRGFLIK
                     RDPEGNNVYQVQMDNRKQILATVTGLSKTALSLCQNSLKAVSTYNAQEDNSFKNSISV
                     QDYAIHTIFCCPLLVNSQPVAFLYLDNLGESGREMYLNEDIISLFQAQAGIALKNAAQ
                     YEAVVRKSHELNEFEALKDEFMAIVTHELNTPLTALQGYVSRLKRKLYSDEDERQELV
                     SKLETSVRKLIISTGDISTMNYYNLARQLAKAPFEIGEILELVHQEVDILSRKRKMFI
                     KMEIEKDLPPLRANWEAVHRMVHNVVLNAIRFTNDFGNVVIGARRSVFPSEKINNRES
                     LVIFVQDNGIGIPAFQLKNIFRKFYELNEIYAHKSGTVEYRSSGLGLGLATAKRIAEL
                     HGGEITIKSKEREGTTVFMILPFKDIGSRGGPA"
     gene            25882..28794
                     /locus_tag="PLO35_01380"
     CDS             25882..28794
                     /locus_tag="PLO35_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78343.1"
                     /translation="MRSMLATQARVLILMMLLFTFQLMGASPREQDDYAYIIELFDMG
                     DHQEALSEMDYFKGSYPSSEFLPYLDYIRANIALKQGDWTLARDLYAPLLKESLHKDV
                     MADVYLNYAIALYNLGDPKNAIPQLLELDLISEHPYYRYWGNVWRGRCYQSLGLLLSA
                     EHEYSKALALDSGDHEIEFEYFKLLLLVQKDTEALAIIARNTEDPVYGRRYRVEWLHY
                     LLYNQRYDEMDEYLAQIEDPEELDSDPVKLLLTRKDLALGRYQEAESRLNQISSSSAH
                     TAYYSALIKLSKGETAQADTIFRQLVRGHDAEIQFLSYLERLKIIYKRDPALATQLLR
                     DYLKSSLPQNFRGQQYILLAEFEFDAGNFSEALRLWTVAKRNDLPADLLDRSEIGIAD
                     AYLGLGQTPLAKDNYNRYLNNYQYGRYRDKAFYQIGRISFDARELPEAKQNLAALIAR
                     YPNSAYKDESFFLLAEIEYFGSDYAAAIELYKSVSDAHPSRQSVQLRLAQCYYHLERY
                     ADAETVLISLGTLFRDYEMVLLEASLAFNRREFETALTLYGEAFNLAKTPIEKQEATS
                     YQAYTLYFLKRFEEASQLFLSLSDLNPSVDSYLFQAARSAYQGRAYKRALDLYDRFVD
                     DYPESPYFLTTLTQIALCYYNLGNPTQAYRDWLNILSRFRNPVSFTPDEISLLREAFS
                     GIQLSLDSMDDLSPTVELMDMIDTFQSEYIRFELSYIIVKLYAGIGDWEETLKQAEEI
                     RRSFPDQKRPELDLLMAESLSRLNQQTQAEELVTQVYAETQSPESLLSLAGLAESTGD
                     WEAAQQKYRELFDIQPTASNWLSLLDISQKNSYHDYDIIWDLGKDFAMDYPQSRVNRI
                     KYLTGKGFYNEAAMMANKILDTETNQFIRAQADAELAMIYYAQDDFTRSASAFKRIRV
                     LYRDYPEIVNNAQFHYILSLIKSGALKEAQLTLWEVQSQLNDDQVIIINDILDRQR"
     gene            28947..30359
                     /locus_tag="PLO35_01385"
     CDS             28947..30359
                     /locus_tag="PLO35_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78344.1"
                     /translation="MPLAQRSTPLFQAAVPVDSLPYFTPSSLPWYSGSILPFYPPLKG
                     RLQARLGTDRSAGIGARLYRLHPMLPLVVLDTDYDFLPQSFDSQRYSLYAQAVPDSSL
                     EFQTLIKYLVSDADAYHNNAFTMIFIHNADSLRDRSWGLRKVRNRLWWENSSQLHTGY
                     RDRHTVPGFWHSHSLSIDKHHLDNQFGSSMGRFGISSAYAIPYDIKGVRKLSAGFMSD
                     MYHLLPLVNIHYEKAIAPRLRLQAGNYPELLPVTRTELLEQYPWAGQARLGRWRMSPL
                     DFRFRLEMDLPGRQPSSSRQSPAQDPDSAQPSISLCLRNSYHYNEPVLRQAGGSAIPS
                     QRFEPLWHNSLSASARIKLPWAWLDQALSLELRHLPHQNGIRAPYSPMITSSTSLRYT
                     SARLEASAELLQLYHRKDQFGGKLPEFIDLNLSASYLLCKRISLVAQLQNLLGNRLFY
                     WKGLPPKKRELSLGVIYLWK"
     gene            30356..31300
                     /locus_tag="PLO35_01390"
     CDS             30356..31300
                     /locus_tag="PLO35_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78345.1"
                     /translation="MNQDKRLERSHGELDSDPVTAERKPELMQTWNNNGPIGLINCLA
                     RYLFGSFNGIQVFLYPGLLLYWLRSDRLVEVISDLLGLRIHYFPLGVFLLLGLYVAFI
                     LLKNRAMSSAKPSQPGGELATFVVDLHVKLLTVLLCFALLFVFAAFTRYFYGIQVPVK
                     LIASWTARALGGVMILYYYLADTWLSPWLNRGHKRQAAVSRTKVYARRHPKTYVAFNL
                     FQIVMVLALARIYVNLVEYIYHPILTLLGSALRLEFELKPILIDGHLALWWNALLTAL
                     AFLISNLGFIPLVWLAKLATDITHPIRPPKIYPHTAEN"
     gene            31381..32562
                     /locus_tag="PLO35_01395"
     CDS             31381..32562
                     /locus_tag="PLO35_01395"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOH78346.1"
                     /translation="MALYIIAGLICVAAIWMITRPVDRASTPVTQESTSARTRDKRKP
                     KSPPHRPAPQTLTPEVTASAGLDPVIVAALGELGIKDGIYKRRTKGGAVIYEAPLDKS
                     VYDLYFANMVLKGRVEKAGGKLESAIEKGQRQSLRFSYPDLDKHWNVELYYDSQPYDK
                     QTPAKTLAIVVDDFGEIGGDLLEGFLNLPKEVSFAIFPGMTNSVSTMDQAAAQGRETI
                     IHVPMEPLGYPQVNPGKNALLLQMTQAEVSRMLHRFIDQMPRCLGINNHMGSLATTDP
                     DLMGYVMEVLKARNKLFLDSRTSNVSVAYQTAQKHHIQAFRNDIFLDSPDVSGTTLES
                     KIRQLEQMSDKKPHLIAITHCHNQAKLVYLVQFINRMRKAGYTLVPLSKSGRYRVPEI
                     L"
     gene            32890..33699
                     /locus_tag="PLO35_01400"
     CDS             32890..33699
                     /locus_tag="PLO35_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOH78347.1"
                     /translation="MNLLQSIFLGIVQGLTEFLPVSSSGHLVLLQRFLRLEMGADVAF
                     EVFLHLGTLLAVLIYFRKLILDLALALFKWRDSPQNQAHRYNRALIMYILAATLGTGL
                     IYLIFGDWFESLYDQPLIVAFMLLVTGAIVFASDYMKGGSIPSVSMGLTRSVIIGLVQ
                     GLAIIPGISRSGSTIGASLFCGLKRKDAASFSFLLSIPAILAANLKEFKVLASLQPSQ
                     FFIYLGGFVSAFGVAWLVISLLINMVQRGNLKYFAYYCWGVGVLSIILLVS"
     gene            33702..>34258
                     /gene="holA"
                     /locus_tag="PLO35_01405"
     CDS             33702..>34258
                     /gene="holA"
                     /locus_tag="PLO35_01405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOH78348.1"
                     /translation="MAVQSINALEFKPSMIGLGRCYLILGTDAWLCDSVLDPIRRELK
                     NRLGADVVNIYGDEVKVPQLVDVLDTFSIFSAQKLVILRNAESLKVAEQKALAAYVSS
                     PSDSQSLVIIATKVDARLVGWKSIKAGCLEIRCDPPRSAYNLKPWLNGMLSKAGRKME
                     EAAIDLFISKVELDFANAANELQKL"
BASE COUNT         8453 a         9284 c         8403 g         7818 t
ORIGIN      
        1 cgtctgcctg tcacgcagaa ggccgcgagt tcgagtctcg tcgctcccgc cattattacg
       61 gacgccatac cggcgtcctt ttctctttta taaaactatg ttgatacact tacagacaat
      121 ccttttccgg cttttcaact cagtgttgga gatcattttt tggcttgtga agcccctgtt
      181 gagacttgct ctgaagcgaa tcgactatca ggacgcgctt aaggcaagcg gagtttctgt
      241 tcagggcggt gttatgatcc atgcggcctc gctgggagag gtaaacgccg tcagttcgtt
      301 gatcagagaa atcagccatc atcttgcgcc ggagagaatt ttgattacca ccactacagt
      361 cgcgggccgg gatgccgctt ccagactgca tccggatatc caggttcgcc tgagtgtcct
      421 ggatgtggcg tccttgagaa agaagcagct ggagatattc gcaccgggat tgatttgcat
      481 tgtagagacg gagatttggc caaacctgct ttatcaggcc gggcaacgca acatcccaat
      541 tctattcctg aatgccagat tagggcagaa atccttgcaa cggctgaaga aatggaagcc
      601 tttgcttcag cgggtcagcg gttctgtgaa agcaattctg gccaagagcg aggatgacgc
      661 catcaggttt aaagagcttt ttcccgcccc tgtccaagtg gcgggaaatc tgaaatttgc
      721 cctcaaaccg cctgtctttg atcccctgga agtcaagagg gaattgggtt atacggatga
      781 ggacctgatc atctgcgtgg gaagttcccg cccgggagaa gaaaatctga tcatgcgcgc
      841 cttttttgag cttcgcaacc aacaccccaa tctaaaactg atcatagctc ccaggcatat
      901 gcaaagagtg gaagaagtta aacgattctg cggcgctacc ggattccatc tctacagcca
      961 gttgaatggc actcccgctt ccgatccggt cttgataatc gaccagctgg ggtatctgaa
     1021 caaggcttac agcatctgcg atatcgcaat cgtgggaggc tcactatttg attttggtgg
     1081 tcataatccg ctggaaccag ctttctactc caaagccatc ctgatcggcc cctaccatga
     1141 ttcctgtcgt gaatccgttc acgaactcgc caaagccgat gccatcgttg ttaccgaacg
     1201 tcagcatcta aacaaacgcc tggccacttt gctggatgat ccctctgaaa gacacgccat
     1261 gggaacaagg gcaaaacaag tgatcaccag gtttcaggcc cccatggacg ctcatctgaa
     1321 cgagatactc aaatggatca actgacgcgc gaagcgatgt attggacccc cttggacgac
     1381 gttgcagacg gcaccgtgag atgtgacctc tgccctcatc actgcatcat cgaagtgggg
     1441 aagaggggac tctgccgatc aaggtttaac cgtgcgggaa agctatatgc tcaaaactat
     1501 ggccaggcta tctcgctgag catcgatcca atcgagaaaa aaccccttta tcactattat
     1561 ccgggctctc aaatcctttc cactggacca aactcatgta acttaagctg ctttttctgc
     1621 cagaattaca ctgtcagcca gcaggaatgt cccagcacct ggctaagttt cgaggccctc
     1681 gaagaatact tcgaactgca tcccaagctt cccaggcgtt tggccatcac ctacacggag
     1741 cctatcactt ggtatgagta catcatggac ctcagcgaga ggcttccgga tctccggatc
     1801 gttcttatca ccaatggata catcgaagcc gagcccctag aagctctcct gccacggatc
     1861 gacgccatga atattgatct gaaatcgatc cgtgaggatt tctatcgcca agcttgcagt
     1921 ggcagcctgg cccccgttct acacacgatc tcaagggctg atcacgaggg cgtgcacgtt
     1981 gaaatcacca atctcctcat ccccgggctc aatgactctg aggaagacat cgtccagctg
     2041 gcaaaaacaa tagccaggat aaactgggag ataccgttac acttttcagc ctatcatcca
     2101 gcctacaagt cttctatccc ggccaccaat ccggaatcag ttttaaaggc ccgagatctg
     2161 gcccttgagc atctacgctg ggtttacgcg ggaaatatcg ggccaggaga gttttgcgat
     2221 acttattgtg ataaatgcct tgagcatttg atctctcgta aacctggcca ctcatggtgt
     2281 gatgtggctt tcgacgatta tccgatctgt cccacctgtc tggaacccat ctggggtcgc
     2341 tttgaggttt aaacacagcg tcaagcagct cacagccgct gatttggttt tgattctggg
     2401 tgtcgttact gcggctgttc tgagcgctat ctggctatgg aagagtgact cgcagaaaac
     2461 agtgtttatc tacaaaaacg atcaactctg gggtgagtat cccctcgata aagaccaggt
     2521 gataagaata gactctcaca acactgtggc catcagcaag ggccgggtgg ctatgaccca
     2581 cgcggactgt cctgataagc gctgtgtgaa acagggattc agtaagacat tgcccattat
     2641 ctgtctgccc aacagggtct tgatcgaaat tctcgatagg gaagaggaag cccctcatat
     2701 cctttattga ccactatgct aaaaatcgct atctacccat ctgatcacgg tttcggccac
     2761 gcggccagaa tgggagctct ggcccaggaa tttattgcgc tgggcatgca ggttcgcata
     2821 aggtcaactc gcccggacta cttgttttca tgcctggaaa gcccattctt ctgtaaagac
     2881 gacgtccgca acgacgttgg cgtgctccat accgctggcc tcaaagccga cttaggcaac
     2941 accaaggcaa gcctcataaa agcgctgtcg ttccggcatg ctatagtgga gagggaagtc
     3001 aactggttgc gaagagaaga gatcagcctg gtaatcgtgg atgttcctta tctgatcatt
     3061 gaggcttgcg cgtatgccgg aatcccggtt atagccgtca caaattttga ctgggtcttc
     3121 atctacgctc gcttattcaa gcgggatatt gagatgagac cggtgatcaa tacaatccac
     3181 agcctttatc aacgctgtga tgcagctttt cgcctgccct tcagttcttc agcgagcatg
     3241 gcggcttttc gctccccaat gaaaagcggc ttgctggcca ggcgcaagga tcggtattgt
     3301 gacttgcgtg aattgtatgg catagagcaa gataaaccaa ttcttacctg cacgttcggt
     3361 ggcgagccgg gcatgagcgt cgatctaaaa tccgtgtgcg cggcatggcc aggaatggta
     3421 atcagcgcca gtgaagaggt ctctgccgcc aatcatattc atgcccccag acacgcggat
     3481 tggcttgatt tgattcacgg ctccaatgtc atcctcacaa aacctggtta ttccactttc
     3541 gcggaagcca cccaattcaa ccgcgggatc atttatcttc ccagacagga ttatcctgag
     3601 gaagaggtcc tgatcagagg gctaaagtta tatctgccaa aactcgaagt gagccaagca
     3661 cccaaaggtg tcaaagcctg gcagcaaatc ttccggagcc tgccatctcc ccaaaaaccc
     3721 caccctgcct tccgaaacca gaattccagg gtcgcggctg atatcatcgg ctcctatctg
     3781 aagctgagac aatcctcgct gaccagcatc ttcgatattg gatcaaacaa tctgaactac
     3841 ctgctttggg atgcccctaa tcatcgcata ctgcacactg ctcatttcac cacccggctg
     3901 gcaaaactgt caaatccggt ggccgtaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnt tatccgattc gcagcagcgt gccagcctga tgtccatcct gccattgatc
     4081 cataacgctc aggacatgca acttgtcctg acaggtctca cagggacttt tctggccgcg
     4141 attgtgaaca agaagtcaaa cgcgctgccc tgggaattcc ataacacccg tctgtcccgc
     4201 cggcaaattg atcagctcgc cgcggcagtt gcaggaaagg tctcagagga ctgggaaccc
     4261 tatctgcaaa cagatacagc gatttcgatc ttggaggcca gcctgggtct ggttgggatt
     4321 attatggaca aatacgaggc tgctgagatc gtggtctgct attatggaat atccttggga
     4381 caagcactaa aacaatgaaa ttaaaacatc taactatcac tggaagagat tatgagattc
     4441 ttccttgaaa cctacggctg tcagatgaac ctggcggaca gcgaactggt gagctcaata
     4501 ttgattgaag ctggtcacct gcctgtgtcc aatatcgaca gcgctgaagt gataatcttc
     4561 aacacctgct cggttcgtca gcacgccgaa caacgcgtat tgggacgaat tgccaacgaa
     4621 aaacaccgca aactgcaaaa tcccggtctg aggattggcg tgattggatg catggcgcag
     4681 cggatgggga agaggctcat ggaaatagaa ccgggagtgg attttgtggt gggagtggac
     4741 cagtatggga tgctgggttc catcctgaac gattccgagc tgccggatga tgatttaata
     4801 agtatggaca gctcgcaaat ctattccagt atccttcctg agcgcgaaaa ccagcattgc
     4861 gcctttgtaa cgatcatgag aggctgcgac aacttttgca cctactgcat cgtgccctac
     4921 gtgagaggaa gggaacgcag ccgccccatg ggggatatct atcatgaagt aaaagacgca
     4981 accctgaaag gatatcgcga tatcacgctg ctgggccaga acgtgaattc atattcccat
     5041 gatggatatg actttccggg tttactcaga cgattgaacg agattgactc tctctggcgc
     5101 ctgagatttg tgacctccca tcccaaagac ctctcagaca gcctgatcag aaccatggct
     5161 gattgtgata aagtgtgcga acatatacat ttaccattgc agtcgggcga tgatgaagtg
     5221 ctgaaacgaa tgaacaggtc ctatagttca gcacattatc tggacatcat ctccaaactg
     5281 cgcaaagcta tacctgatat agctataact actgatctca tgaccggatt tcccggtgag
     5341 accgatctcc agtatgaaaa caccctcagg ttgatgcggg agatcgaatt tgattatgcg
     5401 ttctgcttca aatacagctg tcgggaagga acggaggctg ccgcatacga tcagcaggtg
     5461 ccggaatcgc ttcgcctgga acggctgcaa caaatgatcg atctgcagcg tgaaatcacc
     5521 ctgcgcaaat tccaggccaa gattggtgct caggtagagg tctatgtgga aaacctcagc
     5581 cgcaaatctg ctgatcagct ctctggaaaa acccgtgatt ttaagatagc cgtcctgagt
     5641 ggcaaggccg atcagattgg caaactggtg agtgccacag tgaaaacagc cacggccggc
     5701 accctgatct gcgagtgagc aagcttatgg ataaggcacg ttatgtaaac ttgcgtagca
     5761 ggcagctaac acattgccag gcaaatgact gcgaagcacc tcttccccgc ttcatgtcaa
     5821 gctctctgct gttgtggaag acaaagacca aggagtcaat atgaaaagca tgaccggata
     5881 cggtaaggca aaatactctg ccaatgatat agaactcgaa gttgaaatca agacaatcaa
     5941 tggtcgttac ttagatatga aaatcatcag ccccagagaa ctgagttttt ttgatcactc
     6001 tctcaggcgc aagatcaccc actccctcca ccgcggcacg gttgaaatcc gcgtcacctt
     6061 ttccgatcat cgcgaacctt ccgtccgtct ggacacaacc aagctccgca agtatcatga
     6121 agttgtcaaa tcggcacaga attacctcgg cttggatgag cccctgccat tgcagtttct
     6181 actatcagaa cccggagtca ttgaaatagg taactcactg gatgaagatc cgctactgat
     6241 tgaagccctg gaaacaacgg tgggaagagc gattgacgcc atcatagctt cgacagccga
     6301 agaaggcgct cagataaaag ccgttattat ggattcgctt gccaggatga agcaggcact
     6361 ggatgaaatc gagaaagaga ttgctccctt caagcaagaa ctccttgaga agctcagaaa
     6421 caggattacg gagcttttaa gcaatgtaaa cctggacaat ctggagcaac gattggtgca
     6481 ggaaaccgct atctatgtag ataaatatga tattcaggaa gagataagcc gcctccgaag
     6541 ccacattact accttcgaac gcacccttgc caaggatgat gaacctgaaa taggcaaaac
     6601 cctcagtttc atcactcagg aaatgcatcg tgaagccaac actttagggt ctaaattctc
     6661 aaccaccaag accttcgtgc acgttctcat cctcaaagaa gaggtggaaa aatgccggga
     6721 gatttgtctg aatgtcacgt gagattcttt taaaatggcg atcctggccc ttcgtggaaa
     6781 ggcccaaaac ctcgatcgcg ttggtgcttt ttttgctgat gctggcagct atcctctggc
     6841 atatcaccgt gacaacgtgg aaagctcccc ttttctacta tattgggatg attcttgtcc
     6901 tcaccaacct gttgccctgg ttcataccca ccgaatacga actcgatcac agcgaggtca
     6961 gagtccgcta cctaatcatc accattaaac gcaactattc cgatttcggc tgcttttatc
     7021 aggataaacg tggcattatg ctcagcactt tcaagatgcc ccgccgcctc gatccgttta
     7081 gaggtcagtc attacggttt tccggttccc gagcggaagt ggaagctctc aacgccatcc
     7141 ttttagataa gattggcaaa aaatactaag gtatcacatg aaacaaccag acataaaact
     7201 aattgtaaca gagcactttg cttctaatcc tactcctgcc tcctaccagg agctgatcgc
     7261 cattctccgt ttgcccagaa agcacaaacc tttcttgaaa aaggctttag aagagctttc
     7321 tgccgcgggg aaactcgttt acgtcaagcg ccgctactct cccccactcc ccaaaaaaca
     7381 gcaccgaacg gagcgggtcc ccccggctcc gatgctgagc tccgaactga ttgaaggcgt
     7441 tttcgatgcc acccctctct cccgaaactt ctcatatgct tttgtccgca ccgagaaagg
     7501 ggacttcttt gtaggttctg aagacaccct caatgcctat cacaacgata aagtagccat
     7561 tgaaccccat ttcaggaacg gtaaggcaga ttatggcaga gtccgcagga tcgtgacgcg
     7621 agccaacgaa aggatggcgg gcgatctgag tttcagttcc ggcaaggcta tctttatatg
     7681 ctccaatccc aagatacaca attggtttga gattaccgac acaaacgggg ccaaagaggg
     7741 cgagaaggtc atcctccagg tcagcaactg gggctccccc ctggctggaa aaatgccggg
     7801 aggcaaagtg gtggaagtac ttggcccctc gggagaccct caggtggaac tgatcgccgt
     7861 gatcagacaa tatgatctgc cccttagttt ttgcgatgaa gtgatggaag aggtaaacaa
     7921 tctgcctgac agtatcgatg ccagggaagt cgctcgcagg gaagatttga gagatatctt
     7981 cacgtttacc atcgaccctg cctctgcaaa ggattttgac gacgcgatct ccattcgtaa
     8041 agactccaac ggatacactc tctgggtgca tatagccgat gtggcccatt atgtaaaacc
     8101 cgaaaccagg acctttgatg aagctgtgaa acgcggaaac agcttctatt ttcccaagaa
     8161 agtgattccg atgttgcccg agcggctatc caacctcatt tgcagcctgc gtcccgacga
     8221 agacaaactc tgtatgaccg tgatcaccca tttcgatccg aagggcagga ccaccgctca
     8281 aaaaatggtg gaaagcgtaa tccgttccaa tcaccggctc agttacgagc aagtcgatga
     8341 gctgtttgaa acaggtaaat ccgatttcag taaggacctc aaggacgcgc taagctggag
     8401 tagagagctt tcggccctgc tcagcgccaa gcgcgtggcg gccggttata tcttttttga
     8461 tctccctgag atcgagtatg aatatgatca ggaaggcttt gtgcgccgtc tgaatctggc
     8521 ggaagagacg gaatcccaca agcttatcga aaactttatg ctggttgcca acgaatatac
     8581 cgctgaaaaa ctctcgcagc tctcacccac ttccatctat cgggtgcatg aaaaccctga
     8641 tccccgcaag cttgaaaggc tcggtgagtc gcttagttat tacggtcttg agtatatcat
     8701 cagcgaggac gtcaacaaat ccctccagta cctgctgaac tccatgccca atgccgacta
     8761 ccacaaagta ttcgaccgca tggtgttgcg cagcctcaaa aaggccaaat acactccgga
     8821 acatctgcgc cactttgggc tggggatgga aaactacacc cattttacca gccccatccg
     8881 gcgtctttgc gatctggtgg tacatctgct ctgcaagacc tacgtgatca agagcagcaa
     8941 gctgaagctg gaacgcaagc agatagctct ctatgccgaa atcgcttcca gccaggaact
     9001 gctggccgac gaagcggagc gcgatattga ccgcacttac agcctcgcgt ttatgaaaaa
     9061 gcacctcaac gaacgcttcc acgggatggt cattggcacc aattccagtt ctctgattgt
     9121 gagattaacg gagattccca tcactgccgt tctaaagaca gccatgctgc ccggaggtcc
     9181 ctgggcctat caggaccgcg cgatgcgttt tgtaaataca aagaccaatt actattacca
     9241 gttaatggat aaacttattg ttgatatcgt ggatgtcagt gacgatatat acttggagtt
     9301 agccaatatc gacagcgctc atacgcatgc acaggtcctt agtccccaac ctcagacaaa
     9361 aatcccaaag acgagccctg gtcgctcttc aggtccccga ggcagaaata ggattgacgt
     9421 aatgaccccc tcgccaaaaa gtggacgcca ttcaggtaag aaacgcggtt cgaggagata
     9481 atgaagcgtc ccatcggtat ttttgattcc ggagttggag gtttgaccgt ttataaggcc
     9541 atccgtaaca acttcccgga ggaagacctt atctattttg gtgatacggc cagggtgcca
     9601 tacgggccca aaagcgaaaa caccatcatc gactattcca ttcagaacgc ccgtttcctg
     9661 ttgcagcgcg gcatcaagat actgattgtg gcttgcaaca cctcttccgc tgtagccatt
     9721 ccagtcttgc gtaagatggc ccagatcccc gtgattggcg tgatagatcc tggcgctcag
     9781 caagctgtga ccgctaccag gaatcgcaag atcggtgtga tcggcaccga aggcacagtc
     9841 cgctccggag cttataccaa agcgctgcag gcgataattc ccgaagtgga agttttttcc
     9901 gttccctgtc cgctcttcgt ttcactggca gaagagggct ggcagtcgca tcctgtaact
     9961 gaagcgattg tcagggagta tctaagctat ttcagtgaca aggatatcga tacccttgtt
    10021 ctgggctgca ctcattatcc cttgctggca gacgtgatcc aggccgtgat gggaccaaag
    10081 atcaaactgg tggacagcgc ggctgccatc gcggcctatc tggaaccact gctcccgact
    10141 gaaaatgatg gacagcccgg acaagacagt ttttttgtaa gcgataatga aaacaagttc
    10201 gccgctctcg ccacccggat cctgggcagc gagatcaccc agctcaaacg ggtacgcatg
    10261 tttgaaagct ggtttgaaga ctagaattga tagaaataat acaaggatta acatatggaa
    10321 cccctgattt tgacagcagc catcacggga gctgagacta cccgcaaaga ccagcccaat
    10381 ttgcctatta caccctctga acaagcagcg gaagcagcgg catgttttga agccggggcc
    10441 cgggtcatcc atctgcatgt acgggaagac gatggcagtc ctactcaaag actggcacgc
    10501 tttgaagaat cgattctggc catccgcgat gccgtcccgg aagtgattat ccagatcagt
    10561 actggcggag ctgtcggcga gtcttttgaa aagaggctgg cacctttggc cctgaaacct
    10621 gagatgggca ccctgaacgc cggaacgctc aattttggcg acgatatctt tatcaatcat
    10681 cccaaagaca taatccgtct ggctgaagct ttcaagctct acgatgtggt ccctgaagtt
    10741 gaggtctatg aatctggcat gatcgatgtc attgcccgcc tggtaaaaaa agagatcatc
    10801 actcacacgc cgctgcatgt ccagttcgtg ttaggagtac ccggtggtat gagtggtaaa
    10861 cctgccaatg tgctttatat ggccagccat ctcgcggaag agattccgac tgccacctgg
    10921 gcggtagcgg gaataggacg ctggcacatt ccggcttcga tgatggccat gaccctgggt
    10981 ggtcacatca gagttggctt tgaagacaac atctattacc acaagaattt agtggcggac
    11041 agtaacgccc agctggtagc cagaatggca agaatcgcca cagagattgg acgtcctctg
    11101 gccagccctg ctcaggccag agaaatcctg ggcctgaagt gaggtaatga tgcagctaag
    11161 tgacaacgct ctgatcgtgc tggaaaagcg ctatttcaaa aaggacgggg aaggccgcat
    11221 cctggaagac tgggacgccc tgatcactcg cgtcgctacc aacgtagcgg gcaacgaccc
    11281 cgataaaata atggtttaca aagagcttct ggattctggc aacttcctgc ccaattctcc
    11341 cactctgatg aatgccggca gcgatctgca acagctttcc gcctgctttg tgcttccgat
    11401 tgacgacagc atggacagca tcttcgagac tctcaaaaat gcggccctaa tccataaaag
    11461 cggaggcgga acggggtttt ccttcagccg tctgagggag gcaaattcac gggtccgctc
    11521 caccaatgga gtttcaagcg gccccctgtc gtttctgaag gtcttcaacg cggccactga
    11581 tgcggtaaaa caaggtggta cccgcagagg ggccaacatg gccatactaa acgtcaacca
    11641 ccctcagatt ctggaattca ttacctgcaa ggccgatact aaagagctca ccaacttcaa
    11701 tcttagcgtt ggcatcaccg aagagtttat gcgggcggtc tatcaggacc aggaatatga
    11761 gctcatttca ccccataccg gtgaggcgca gagatctcaa aacgcccgtg aagtctttga
    11821 actgatcgtg gaaatggccc acaaaaacgg agaaccgggc atcgtatttt tggataggat
    11881 caatgccgat aatcccactc ccgccttggg ccagattgaa tccaccaatc cctgcggaga
    11941 gcaaccattg ctgccaaacg aagcatgcaa tctgggctcg ataaacctgt ctttgttcgc
    12001 cagcggagat actgtggatt gggccaagct caaagccgtg gttaggttga gcgttgattt
    12061 tctggatgac gtgatcgaag tatcccactt tccgcttcct cagatcgatt ctatggtaaa
    12121 ggccaatcgc aagatcggcc tgggtgtaat gggctggagc gatttgctct accagctcaa
    12181 actcccctac gccagtgaag aagctgtagc gctggcctcc agagtgatgg aattcatcga
    12241 tttttccgcc aagcagcgct ccatggaact agcccgggat aaaggcagtt tccccaattt
    12301 cccggacagc agctacgcca atggcatcaa caaccgcaag caaatcgatc aggactggga
    12361 aggcctctca aaggctatag caatcaatgg gattcgtaac gccaccgttt ccactatcgc
    12421 tcccaccggg actatttcca tgatcctgga tacctcgagc ggaatcgaac cccagttttc
    12481 tctggcctac gtcaaaaacg tgatggacgg agaaaaactg ctctatgtaa acaaatggtt
    12541 tgaaaaggcc ctcgatcgcg aaggcctgct tcgtcaggat atcctcgaac gcgtatccca
    12601 gggagaatcc ttgcatgatt tggaagaaat cccccaagcc atccgggacg tctttcaaac
    12661 cgctcatgat atcagccctg agtggcatat ccgcatgcag gcagccttcc aggcttacac
    12721 ggacaacgcc gtcagcaaga ctatcaattt tccccacgat gccacccggg aagatatcca
    12781 cagcgcttat gaaatggcct atcagctagg ctgcaaaggg gttacggttt acagggacgg
    12841 cagtcgcgaa aaccaggtcc tcagcaccgg gtccaccccc aaaaccgaca aaaaagttgc
    12901 tccccgcacc cgtcctgaag taacccatgg catcacacaa aggctggaaa ccggttgtgg
    12961 acacatgtat gtaaccatca acacagacga acaaggggcc tgcgagatat ttgtccagat
    13021 gggcaaagtg ggaggctgcg cttccgctca gttggaagct atcgccagat tgtcatccct
    13081 ggccttgagg tccaacatcc ggttggaagc catcattcgc cagctcaagg gtatacgctg
    13141 tcaatcacct atgtggcaca aaggccaaat gattacttcc tgcggcgacg cggttggccg
    13201 tgctctggaa ggctttctca ccctgcataa aaatggggat gtgaaatccc tgcaaataga
    13261 gaccgatgcc ggattaatgc agatcccggt caaatcatcg ggttccgccc tctgccccga
    13321 ttgcggatcc acaatcgagc attcggaagg atgcttgatt tgcccatcct gcggttggtc
    13381 caaatgctaa aactttcagg ggaattgagc ttccccttat ccaaaatctt aacaggagat
    13441 aatcgatgcg gtgtccccat tgcaacaccc gcctggccag tcatgacgtc tggtgcctga
    13501 actgccatcg ccaaagcggt ctgatcaaaa acgaactttc cgcctggcag agcttcaagc
    13561 ggagttggaa cgcctatacg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nntcctggag tatgagcttg accgccaata ggatacgcag atgagcagaa aagagatcat
    13741 ctcaatagtc atccttgtcc tggtggcagg atatggtgcc tggcagtact ttcacagctc
    13801 gtttacccag accaaatctc agcatctcat ggataccgtt gtccgcgtca ccgccacctc
    13861 aaaaagcaaa aacatcggca cccgcatcga ttccgttttt tcctacatca agcgcctgga
    13921 agacaaactt aacgactatt cggaagattc ctggctgtgg aagatcaacc attcggatca
    13981 gacgcagttt gccatggatc cggacattta cgagctgctg gttatctcag acagtctcta
    14041 taaaattact gacggggggt tcgatccaac tatcaagccg gtttacgatc tctggggctt
    14101 tggttccagg ccctacagtg ataccgattc ccttgcaaac gccgtaccgg acagcctggt
    14161 gctggccaag acacttgaac aggtgggttt tgacaagatc agattcgatg cccggactct
    14221 ttataagcct gtggaagtaa aactggcttt tggtgccatc gctaaaggct atattctaga
    14281 taaagccaga gcatacatga aaagcatggg aataatttct ggtaacatcg attgccgcag
    14341 cagtatgacc ttctacggtt cgtccctgcc ccagcaggtc tacattcagc atcccatgtt
    14401 aagccaggac gactatatcg cctctttcaa gatttataac aagtcggtag gcacctccgg
    14461 aaactatcag cagttctttg agctcaacgg gatcagatat catcatatcc tcaaccccaa
    14521 gactggttat ccggttccca acgtctattc ctgcaccgtt atccatccct ccgcggcttg
    14581 ggctgacggt ctttccaccg ccctatttct ggttccacct gatcgtgcga tcgagttggc
    14641 ccgctcgatt cctgattgcg acgcggtgat ctatttcgat caggacggca gcacggtttc
    14701 ccttaagacc atgggcatga aggatatcgg cttcagcgag aagatatgac cattccagac
    14761 cttcagtccc aaaccgatca ccgcaggatc aacatcgaca aagttggcgt caaatcactg
    14821 cgctacccta tccttgtgga agatcgtgaa aacactgctc agcacacagt cgccgatctc
    14881 aatatctatg tggagctgcc tcaccaccga cggggtaccc acatgtccag attcgtggaa
    14941 atcctcaatc attaccatca ggatatgatc atcggtcagc tggaccgctt tctggatgag
    15001 ctcaagacgg tcatgaaagc cgaagcggct tacgtcgata tcagtttccc atattttatc
    15061 cgtaaaactg ccccggtctc caagatttcc tccctgatgt gttatgactg ttttttcaat
    15121 gcctgtatgc gcgatgaata tgaactctgg atcggagtgg tggtccccgt caccacgctc
    15181 tgcccctgct cgaaggagat cagtcagtac ggcgctcaca accagcgctc tcacatcact
    15241 attaagctct gttaccgtga gtttgtctgg ctggaagaat tgatcgctct agcggaatcg
    15301 gtggcttcct gcgagatttt tcccctcctc aagcgtcccg acgaaaagta tgtcacggaa
    15361 cgggcttaca ataatccccg ctttgtggaa gacattgtca gagagctcac ccagggcctc
    15421 aaggccgacc cgagagtaaa aaagtttatg gtggaagcgg atagctttga atccatccat
    15481 gcccacaacg cctacgcgaa ggttgtgtgg gacaccgaaa cagatcttcc cgcccgcccc
    15541 aaaacccgga aatcctgatg ccaggcctgc acatccagcc agcagaatcc tttgttctgc
    15601 ccaacggcct gaaagttatc ctgcataaag accccagcga gccgattgtc tgtctgcagg
    15661 ccgtggtccg atctggaagc gccaatgaac gcgcacccga agccggcttc tcgcacttca
    15721 tcgagcatct cgccttcaag agcagtaaac gctacggatt caatcagatc agcagcaccg
    15781 tatccagatt gggcggttcc ctgaacgcct ataccgattt tgacagcacc tgctattatc
    15841 tattgctccc ttccgagcat gtccattcag gacttgagat catatcagag ctgctttccg
    15901 gtgccaagtt tacggctgtg gacgtgacta cggaaaaaga gatcatcctg gaggagatcg
    15961 gtcaatactc agatgatccc gaaaccgact ttctggagtt catccaagcc cgctattaca
    16021 agcgttgtcc cctccgcaaa cccattgtgg gcacccccaa agatgtacgt agagccacgc
    16081 acgcatccat gaaggctttt tacaaaagac gttatcaccc ctccaactgt tttctggtga
    16141 tcagcggcgc cgtcgatcca gccaccctca aagcgtcggt cgatcatctc tttggggcct
    16201 ggaagacacc ctcgcgtgcc acccgcatcc cagcttcaga atggctggag ccggagtttg
    16261 ggcgctccgg tctgcactgg cagaaggcca atcaggagct cttcgcgctg gtattgccgg
    16321 agctctcgga attgcacccc atggcagacg cgctcttgat cgctattcnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt gccggagctc tcggaattgc accccatggc
    16501 agacgcgctc ttgatcgcta ttcggtatct agctatcgga agggcctctg tgctccacaa
    16561 gatcctggta gaagaaaaga agctatgttc cgctgtcaga gtaagttccc tctgcggtgt
    16621 aatgagcgga gcttctgcgg tcacctttgt tccatcccgc aaaatggcaa ttcccgctat
    16681 cgcgcgcgtg atccgtcagg aatataacaa gttatggcat cttggcgttc ctcaggctga
    16741 gtttgagctg atccgcaaag acgtagtcca tggctggctg tttggttttg aagcccgcga
    16801 aaatatcgcc aacctgctgg ccgcggagga actgctggga gactaccaca gcctctacga
    16861 atacggggac cgcatcgaca aactgagccc cggggacgtg agtgccagcc tcaggaaata
    16921 ctggcatcct atccgtctgg ccctggccta tcgcggtccc aaacaacttg tccttccaag
    16981 tgatttgatc cccagccatc cggattacct gcactttccc aaaaaagact tgggcaccaa
    17041 acgcccatcc ggtgagatca gctttcctgc cctgtccaat caagcaacaa aacccagtcc
    17101 agagctggtc cgcgtggatg aacgtcactg gcagggtatg ctgcccaacg gaatccggtt
    17161 tgtattcaaa aagctcgctc aaggccccat cggcagcttt gctgtcacga ctcccgcgtc
    17221 ccagctttgg gagggccctt ctgagcgggg tcgcaactac ttcctctcca ccatgatgct
    17281 ctatcagagc ggcaacctct cccatcaggc gattatggat tcgtcccgtt ggctgggact
    17341 caatatccgt gtctcacaca atgtggacac caccactttc aagggtaagt gctttgtgga
    17401 cgatctggcc catacactcg atctgaccgc cgagcttttg aacaacccaa aactcgatca
    17461 ggcatatctt tacactctga aagcggctgc ctcagatgca ttgcgtcgcg agcgcgacta
    17521 cccacccagc tacggatatc tgctgtggct gagggctgtg tttggcaaga aaaacgcgct
    17581 ggaacgctcc accggcagcc ttaccgatat ccgggctctc cgtctcagtg atctaaacga
    17641 ctggtatgat agatactatc acgctgcaaa ctattccctc gcgatctgtg cgcctctgga
    17701 agctcaggag gtttgggacc aagtgaaccg gagcttcgga agaattcact ttcaggagca
    17761 tccctcccga gggacaactc acccgatata cttcaaaaac ccccgccaaa tcgtctcttc
    17821 tggtgccaat ggacagtgtg tcatccatat cggtgctccg gccaccacct ccaaagacaa
    17881 ggtccacacc actgccagcc acatgctgtc ccagattatc gggggcgata tcaactcccg
    17941 cttttttgat ctgattcgcg aacagcgagg cctggcatat caaaccggca tggacgtcgt
    18001 gactctggac ccactgggct actggactgc ctacgctttt tgtggaaagg aagatcaaca
    18061 gcgttgcctg gacctgatga aggtgattat ccaggaggtt gccgagcatg gaattaccgc
    18121 cgaagagctt gagcttgcac gcaactacat tattgggatg catcgctttg acagcgaaag
    18181 cgtgagctat caggcctccg ccctggccaa ccttctcgct ctgggttatc cgctcagcca
    18241 tctactggaa cgaaatcagc ggatagcgtc cctcgatctg gacaccatga atctggtggc
    18301 tgcccgctgg ctgcgttccc aggatcaata tatccatata ttacaatgat ttgccttggt
    18361 ctggacatcg gttcacgcaa caccaagctg gtaatttttg atcagagcgc tcagagcatc
    18421 gttttctctg cctggcaggc taccgacatc gaccccctga tcagcgtcag ctctctactg
    18481 gacaaagctt taagcgccac agatatgcgt ctggatgatt tcgctggccg ggcggtaacc
    18541 gggtatggca gaaaactgtg gaaaagcgcg gaccgggttc tttcagagat atcatgtcac
    18601 gcggcgggaa tgcattacca ctatccagat gtccgcacga tcatcgatgt gggaggtcag
    18661 gacagcaaga tcatctgcct ttcaaacgcg gctaaagtgt tggatttcag catgaacgac
    18721 aaatgcgcgg ccggtactgg gcgcttttta gaaatgactg ccatcagact gggagtgagt
    18781 gttggccaac tggccggttt ggcctcacaa tcctcttctg agctggcttt gaactccact
    18841 tgcgtggttt ttgccgaatc tgagattatc gggctgatgg cagcccacgc tagcgccgcc
    18901 aacatcgcca gagctgtgca tcgctcagtg gccaaacgcg tcttcagtca gttggctgct
    18961 ctcgaccacc atccccccta tgctttcaca gggggtgtgg cacaaaacgc tgatctggcc
    19021 ttctgcctcc aaaacgaact gcaggtccac ctggccatcg cacccgatcc cgaaatcacc
    19081 ggcgctctgg gtgcggcaat cctcgcggtc aaatgattct gaccgactat catcttcata
    19141 cagaatatag ttgggatagc caccttaaag cagagaatct tatagccaaa gctatctcgc
    19201 tcaaatacga tgctatagca atcactgagc atttggacct tctgccctgg gaactgggtg
    19261 agaatggtct ggtatcgctg agaaaatacc gtgaccggct atcggaactg caggccagct
    19321 atcctcggct tcaagtcctc ggaggcatag aaatcggaga ctaccagtcc gtgcgcgatt
    19381 ttgccagtga tctgatctct gagtatgatt ttgacctcat cctgggggcg gtgcattttc
    19441 tcgttgaccg cactaatgtg gccatccctc ttccccatcc gcttgataaa tctcagatcg
    19501 aagcatacta ccgcctcaac ctgcgtctcg taaatgattg cgatatagat attctggccc
    19561 atttgggggt ttatcagcgt tattacaaag ctgtgccgga agagagcttc gccctgccaa
    19621 tcatcaagga cattttcagg acgctgatcg agcgcggtat cgctctcgaa attaactact
    19681 cggcctatcg caaaccgctc ggatgctgcc ttcccaatcc agctgtcctg gacctctatc
    19741 tcgaaatggg cggacgcctc ttcagtattg gctctgacag ccaccatctg gatcattttc
    19801 atgattggcg ccatctgttg cccggttgga ccagagagct ggtctttacc cacaagcgtt
    19861 tactggcact cccatcgtga tccctgctcc ttacccgccc ttgtgatgcc gtgcgctccc
    19921 gggcgaaaaa cgccggaggc ggcaccacat ctccacggca gctgatcagg caggaactgc
    19981 cagatgtttg ccgtaaaact aattccccaa ttggaaatgt aagccaatcc tagaccaatt
    20041 ttatgggtat cccgcctgca agtctctctg aaaccgctta cgataaacga agctaatgat
    20101 ctgtaatcag gcacttaagc tactaatact gtttgattca ttgagagaat atccggcgaa
    20161 atcaatatga gaccagaatc tgcccttaaa attgtagttg acatcaggcc tcagttttaa
    20221 attgtagggt taccgattgt ttggggagaa agccttaatg tatatagatc atagatatga
    20281 agtgctggaa agcctgggct ctggcacctg ggccaaggtc ttcaaggtca aggatatccg
    20341 cagcggccat ttttatacgc tgaagttgtt ccaatacctt tcctcagagg acctctatgc
    20401 tcgcttcagc gctgaagaga tgcatcacat caccaggatc gaacacccta atctgagcca
    20461 tgtggtcgat tttgggcatg tgggcgatca tatctacttt atcagcgact ggtttgacgg
    20521 cactccgctc accgctttcc gtttcagcaa aggcaagatt gacctgctct acgatctggt
    20581 agcgcagtgc gcctacgcgc tgcacgccct ccacacgcag aacattctcc acaaggacct
    20641 gaagccagaa aacattatct acagacagac aggtaaagcg ttggaggtaa agctgatcga
    20701 ttacggattt gccgtccacg aacagaccag ggataatcag ttggtgagtg gcacgctccc
    20761 ctatgtggcg ccggaagttt acctgggcca gagaaccggt ccggagagcg atttttatgc
    20821 tatgggagtt atcctctacc gcctctgtac tggcaccttc cccttcaacc tggaccagat
    20881 atctgccttg atgagcggta gccagcaata ctttatcccc atctttccct ccgagctcaa
    20941 caaagatatt cccctggccc tggaaaagct gatcctgcga cttctggaaa ggaacccgga
    21001 ccatcgcttt agcgatgcca ttgagctgat cgtttatttg aaccgcgttt cgggtcggtc
    21061 ttaccccttc tcgctcgaat ggtcgctggt taacactctg aggttcaaca gctatatcgt
    21121 gagggagcgc tacagccatc aactgctgga ttacctgcct gccgtccggg ccggcaatgg
    21181 caagatagtt tctctgattg gcggggatgg actcggcaag gacagtattc tctcgctctt
    21241 tcgctaccat ctgctgggtg gagagtgctg gctcttcgat tacagctgca cccggacaga
    21301 ccacgaggcc tttttcgcct tgatcaaaga atatttgcgt tccctgccgg aggacgagca
    21361 gcagaaattc cccagtctaa gcaggatatc agacaagttc aaggcttatc tgaacgggcc
    21421 tgagcaagcg gccaagagtt acagccaggg ccaggaagag ctgcggcagg atttctcggc
    21481 tgcccgtgat ctcctgatgg agctctccac ccgcaaaccc attatcttta tcattcggga
    21541 cttccagtat gtccatcgcc acacggtgga ctttatcaat tatctctcac ctcagctggt
    21601 gcggcatcgc gtgttggtgg tgctctcctg caacgagttt aacaaggtcc gccagatcga
    21661 aaacacggtt ttgaatcata tcccgatgct cgatatggat gaggcacggg attatatggc
    21721 ccggctgttg agcactccgg tccctgaaga cctggtccgg gccgtatatc atcgctctgc
    21781 gggaaatccc cattttatca gggaaatcct ggtggacctc accctcagaa aggagatcgg
    21841 gcaccctaaa gaagaaaggg acaataaaat agagaacggg gaaagctctg acaagaccag
    21901 agtgaaccaa cccggaacac tatccgtccc taccggaagg agttatcact tcccagccag
    21961 cctggcggga tatcaactgc catcccgcgt cctgcattcc atctactccc ggatgagcca
    22021 catcactgag ctaaactacg cgcgcttgca gaaactggcc atagcacggg tgccgctctc
    22081 aagggaattg atcatttaca tcctcaagct cagcgatcag gagctttact ccctgatcaa
    22141 tgaagctact tacaatgaga tcctggtaaa acacggcaag ctatactatt acaccttccc
    22201 ggaggcccgt cagcgctttt ttgacgaaaa ctcccgccga gcccatggat tagtctcccg
    22261 ccgagtgcta aaatactacg cgagtcgatc tatagaagat accgaaacct gcctgggcct
    22321 gatcgaaaac gcgattctgg ccggggacgc tctgagcgcc agacgctatt acctgaggct
    22381 ctatcacctc caaaatgatg attttgaaca ggaagaagcc tatgaggcca ttatcaacgt
    22441 cctcaagatc gatattggcc gttttgaggg cctggttcac actggcaagg caaacggcga
    22501 agctgattgc tccgtggttt cggaagtcag cgtcccgccc gcggatatca tccgggacct
    22561 cagagagttt catgaaaaga cagaggccac agggttcttt cgcagggccg gattcattgt
    22621 ggacaacgct tccgccattc cggagaccag cgaaaagtat ctggtgctgg gcactttaaa
    22681 aatgctggcc gaagagctcg tggaggcacg ggccctgttt caggccgctc tggatctttc
    22741 catgaccgga cggcaaagag tgttgagcta tctttatctc tccagagtcg cgtcgcgggt
    22801 ggatcacaat ctggcgaaga gctatctgga ccagatcgac ccggctgacc tgaatctgga
    22861 actgaagatc atctacacgg accggttggc cgtctattac gcggccaaaa aagaaccagg
    22921 gatggcgatc aggaccatgg agagctttct ggaatccctg ccccctgatc acgataccag
    22981 agcgatgatc aatctggcgg ccctgcataa cgacctggga gtcttctaca gcgatatgaa
    23041 aaatatcacc gaggcggagg agcatctcaa tatcgccttg aatatctgga aacgccacaa
    23101 tatcaaacgt tatctgggcc tgatctacaa caatctggca gacctctgtc tcaaacaagg
    23161 cctcaccgtg aaagcccagg aactctcgga actgggctac agctattccg cggagctcaa
    23221 cctcacaatg ggccaggctt tggcgctcct gaaccaggga gaggccttta tcaaaatggg
    23281 cgagtttggc accgccgagc aaaaactcag cgaatcgctc catctggtcc aatcgatcgg
    23341 aagcactcag tatctggtat ccatccagcg caatctggcc ctggcaaaaa gcaagatcaa
    23401 gggttttgga tatttctatc ggtttatcag cgacatcgag ccccaactga tgcaaggaca
    23461 gatcatcgag gtcaatcccc tggtcaagac ctacttttac tacctgcacg agactgccaa
    23521 cctcaaaaag ctgaggcgtc tgatccgcaa aaacgtgcag ataaactata aacacatcca
    23581 cgaagaggag ttttatcata atatcctcag cctcatcgct ctcagcggag gcgattacgc
    23641 gctggccctt tccgaattgc gacaggcgat gcactttgcc ggcgagatca acaacaacta
    23701 cgcgattgcc gttttcaacg tgctgcaggt gagatgtcta tatggtctca atgatctggc
    23761 aaaagcacgt gagctcgcag aaactgcact gatcacgatc cgggccaaca gctatcgcta
    23821 ttgggagatc aaccttcaga ttcttggctt gaaattggac ctgatgaatc cggaaacccc
    23881 gctgaggggc attctgagaa aggctgaccg cgtcctggca agttgccgcg aattccagta
    23941 ttaccaactg gaagtggaag cctggcaggt gaaactgcag atcatctccg aactcggaat
    24001 tgaaagcaaa gccgccagcg aatttgagca ataccggaga ttgctggatc tgatcacggc
    24061 ggatatcgat cccgaagacc gtgaaaacta tctccatatc aatcactacg atcaaaaaaa
    24121 cctcaagaag ttcgccgccc tgccattggc ttccagacgc aaagacctgc gctaccgctg
    24181 gaacgacctc ctgtataaca tcgccaatgt gaacagcatc gaccgggtaa aattcctgat
    24241 cgaaaaaggg ctggaccagg tgatctgccc agcgcggttc aagctgatgg tcttctcgga
    24301 caagatctca aacttttatt gcttccactg cttcaattgc ggtagagaca ccatggtccc
    24361 ccctgaactg ggcccgctga tcgaaaaagc cttcgggcaa gacagcatcg tatcctgtga
    24421 atataaaggc cagaataccc agataatccc gctcgtgtcc ggctccaaac gcatcggcta
    24481 tctgctgatg tcggacgacg gtgagctgga attcacccgc aacgagctca ctttactcaa
    24541 aaacatcaaa caacatctca ctgccctgat tgtgagagtg aatgactaca gccagatcac
    24601 tcaccgggtc tccaaaatga atgaattgat ggccatctca aacgaattga tggggatagt
    24661 ggagatcaac gatctgcagc gggagattgt ttccacggcg atcgacttta ccaacgccac
    24721 ccgcggtttt ctgatcaagc gcgatccgga aggcaacaac gtttaccagg tccagatgga
    24781 taatcgcaaa cagatcctgg ccaccgttac gggattgagc aagacggcgc tgagcctctg
    24841 ccagaactcc ctaaaagccg taagcactta taacgcgcag gaagacaaca gcttcaaaaa
    24901 ctccatcagc gtacaggact atgccattca caccatcttt tgctgtccgc tgctggtcaa
    24961 cagccagccc gtagcctttc tgtatctgga caatctgggt gaaagcggcc gggagatgta
    25021 tctgaatgaa gatattatca gccttttcca ggcccaggcc gggattgcgc tcaaaaacgc
    25081 cgcccaatat gaggcagtgg taagaaaaag ccacgaactg aatgagtttg aagcactcaa
    25141 ggatgagttt atggccatcg tcacccatga gctcaacacc cctctcacag ccctgcaggg
    25201 ctatgtctcc cgcctcaaac gcaagctcta ctccgatgaa gatgaaaggc aggaacttgt
    25261 atccaagctg gaaacctccg tgagaaagct tatcatcagc accggcgaca tcagcaccat
    25321 gaactattat aaccttgcca ggcaactcgc taaagctccc ttcgagatcg gcgagatcct
    25381 ggaactcgtg caccaggaag tggatattct atcacgcaaa cgcaagatgt ttatcaagat
    25441 ggagatagaa aaagacctgc cgcccttgcg cgccaactgg gaagccgtcc acaggatggt
    25501 acacaacgtc gtgctcaacg ccatcaggtt taccaacgac tttggtaatg tcgtgattgg
    25561 ggccaggcgc tccgtctttc cttctgaaaa gatcaacaac cgtgagagcc tggtgatctt
    25621 tgttcaggac aacggcatcg ggattccggc ctttcaactg aagaatatct ttagaaagtt
    25681 ctatgaactg aacgagattt atgcccataa atcgggcacc gtggaatacc gctccagcgg
    25741 tctgggactg ggtctggcca ctgccaaacg gatcgctgaa ctgcacggcg gcgagatcac
    25801 gatcaagagc aaggaacggg agggaactac agtgtttatg atcctgccat tcaaagatat
    25861 tggcagccgg ggaggtcccg catgagatca atgcttgcca cacaagcccg cgtgctcatc
    25921 ctgatgatgc tccttttcac ctttcaacta atgggagcga gccccagaga acaggatgat
    25981 tatgcctata taatcgagct gtttgatatg ggagatcacc aggaagcgct atctgagatg
    26041 gactatttca aaggcagcta tcccagcagc gagtttctgc cttacctgga ctatattcgt
    26101 gccaatattg ctctcaaaca gggagattgg accctggcgc gcgatcttta cgcgcccctg
    26161 ttgaaggaat cactgcacaa agacgtgatg gctgacgtct atctgaacta cgctatcgct
    26221 ctctacaacc ttggtgaccc caaaaatgcc atacctcagc tcctggagct ggatttgatc
    26281 tccgagcatc cctattaccg ctactggggc aacgtctgga gaggcagatg ctaccagtcg
    26341 ctgggactgc tgctttcggc cgagcatgaa tacagcaaag ctttggcact ggactcaggt
    26401 gatcacgaga tcgagtttga atatttcaag ctgcttctgc tggtacagaa ggataccgaa
    26461 gctttggcga tcattgccag aaataccgaa gacccggtct acggcagacg ctaccgggtg
    26521 gaatggttgc actatcttct atacaaccag cgctacgatg agatggatga gtatctggcc
    26581 cagattgaag atcccgaaga gctggattca gatcccgtca agcttctgct cacccgtaag
    26641 gacctcgccc tgggccgcta tcaggaagct gagtccaggc tcaatcaaat cagttccagt
    26701 tcagcgcata ccgcctatta cagtgctctg atcaagctca gcaaaggtga aactgctcaa
    26761 gccgatacaa tcttccggca actggtaagg ggccatgatg ccgagatcca gttcctttcc
    26821 taccttgaaa ggctgaagat catctacaaa cgcgatcccg ctctggcaac ccaactcctg
    26881 agggattatc tcaaaagctc tctgccccaa aactttaggg ggcagcagta tatcctgctg
    26941 gctgagtttg agtttgacgc cggaaacttc tccgaagctc tcaggctctg gactgtggcc
    27001 aagcgcaacg atctgcctgc cgatctgttg gaccgctccg agattgggat tgcggatgct
    27061 tatctgggcc tgggacagac gccactcgca aaagacaact ataaccgcta tcttaacaac
    27121 taccagtatg gacgctatcg ggacaaggcg ttttaccaga tcggcaggat cagctttgac
    27181 gcgcgcgaac tacctgaagc aaagcaaaac ctggcagcac tgatcgcccg ttatcccaat
    27241 tcggcttaca aggacgagag ctttttcctg ctggctgaga tcgaatactt cggctcagat
    27301 tatgctgccg ccattgaact ctacaagtcc gtgagcgacg ctcatccttc ccgccagagc
    27361 gttcaactga ggctggcgca gtgctactat catctggagc gctatgcgga cgcggaaacg
    27421 gtactaatca gcctcggcac cctctttagg gattatgaga tggtgctgct ggaagccagt
    27481 ctcgctttca accggcgcga gtttgaaacc gccctgaccc tctatggaga ggccttcaat
    27541 ctcgccaaga cccccatcga gaaacaggag gccaccagct accaggctta taccctttat
    27601 ttcctcaagc gttttgaaga ggccagccaa ctgtttttga gcctgtcaga cctcaatccc
    27661 tccgtggata gctatctctt tcaggcagcc cgttccgcct atcagggcag ggcctacaaa
    27721 cgggctttgg acctctatga ccgttttgtg gatgattatc ccgagtctcc ctattttctt
    27781 accactctca cccagatcgc cctctgttac tacaatcttg gcaatcccac ccaagcctac
    27841 cgggattggc tgaatatcct ctcccggttc agaaacccgg tatcatttac cccggatgag
    27901 atttcactgc tcagagaagc tttcagcggt atccagttga gtctggacag catggacgac
    27961 ctctccccaa ccgtcgaact gatggacatg atcgatacct tccagagtga gtatatccgc
    28021 tttgagctca gctacatcat tgtgaagctc tacgccggca taggcgattg ggaagagact
    28081 ttgaaacaag cggaagagat caggcgcagc tttccggacc agaagcgtcc tgaactcgat
    28141 ttgctaatgg cggaatcact ttccagactc aatcaacaga cccaggcgga agaactcgtc
    28201 actcaggttt acgcggagac tcaaagcccc gaaagtctgt tatcgttggc ggggctggca
    28261 gaaagcactg gagattggga agccgcccag cagaagtaca gagagctttt cgatatccag
    28321 cccactgcat ccaactggct gtccctgctg gatatatccc agaagaactc ctatcatgac
    28381 tacgacatca tttgggacct aggaaaagac tttgccatgg attatcccca gtctcgcgtc
    28441 aaccggatca aatatcttac aggtaaagga ttctacaacg aagcggcgat gatggccaac
    28501 aagatccttg atacggaaac caaccagttc atccgcgctc aagcagatgc ggaactggct
    28561 atgatctatt acgctcagga tgatttcacc cgctcggcgt ctgccttcaa acgcatccgg
    28621 gtgctctaca gagactatcc cgagatcgtc aacaacgcgc agttccatta catcctcagc
    28681 ttgatcaaaa gcggagcgct caaagaagca cagctcactc tctgggaggt gcagagccag
    28741 ctcaacgacg atcaggtgat cattatcaac gatatcctgg acaggcaaag atgaagcggc
    28801 gattggcgag caacctggtt cagggcttca gacttccagc tttgaaggcg atcatcgttt
    28861 gtctgttgat aatatcgatc agatgtttgt ccgcgcagac caggcaacta ccggttgtgg
    28921 agatcatggg agagggaaag gtccaaatgc cactggccca gaggagtacg cccctcttcc
    28981 aggctgctgt acccgtggat agcttgccat acttcacccc aagctccttg ccgtggtatt
    29041 cgggaagcat cctgcccttt taccctcctc tgaaaggaag gctgcaagcc agactgggaa
    29101 cggacaggag cgctggcatc ggcgcgcgtc tctatcgcct ccatcccatg ctcccgctgg
    29161 ttgtgctgga tacagactat gactttctcc cccaaagctt tgatagccaa agatattcac
    29221 tttacgcgca agccgttccg gacagcagtt tggaatttca aaccctgatc aagtacctgg
    29281 tctcggacgc cgatgcctac cataacaacg ctttcaccat gatcttcatc cacaacgcgg
    29341 acagcctgcg ggaccgctcc tggggactga ggaaggtgag aaaccgcctg tggtgggaaa
    29401 acagctccca attgcacacc ggctatagag acagacatac cgtgccgggt ttttggcaca
    29461 gtcatagcct gagcatagac aaacaccatc ttgacaatca gtttggcagc tccatgggca
    29521 gatttggcat cagttccgcc tacgccattc cctatgacat caaaggtgtc agaaaacttt
    29581 ccgctggatt catgagtgat atgtatcacc tgctgcctct ggtgaatatc cattatgaga
    29641 aagccatcgc tcccaggctc agacttcagg caggaaacta cccagagctc ctgccggtca
    29701 ccagaacaga gctgctggag cagtatccct gggctggtca ggcccgtttg ggtcgctggc
    29761 ggatgagccc tcttgatttc cgatttaggc ttgaaatgga ccttccaggc cgccagccct
    29821 ccagctcccg ccagtctcca gctcaagatc cggactcggc ccaacccagc ataagcctat
    29881 gcctcagaaa ctcatatcac tacaatgagc ctgtcttgag gcaggccggc ggctcggcaa
    29941 ttcccagcca gagatttgaa cccctctggc acaacagctt aagcgcgagt gccaggatca
    30001 aactgccgtg ggcctggctg gaccaggcgc tcagcctgga gctgcggcat cttccccacc
    30061 aaaatggtat cagggcgcca tacagcccca tgatcacctc cagcaccagt ctgcgttata
    30121 cttccgcccg tctggaagcc agcgccgaac tcctgcagct ctaccaccgc aaagaccagt
    30181 ttggcggtaa gctgcctgaa ttcatcgatc tcaacctttc agccagctat ctgctttgca
    30241 agcggatcag cctggtggcc cagcttcaga acctgctggg caaccgcttg ttctattgga
    30301 aaggtctgcc gcccaagaag cgggaactga gcctgggagt gatctacctg tggaaatgaa
    30361 ccaggataag cggctggaga gaagccatgg ggagctggat tccgatccgg taacggctga
    30421 gcgcaaacca gaacttatgc agacctggaa caataacggt cccataggtc tgatcaactg
    30481 cctggcccgc tatttgtttg gaagtttcaa cggcatccag gtgtttttat accccggcct
    30541 cttgctgtat tggctgagga gcgaccgcct ggtggaagtg atctcagatc tgctgggcct
    30601 caggattcac tattttccgc tcggcgtctt tttgctgctg gggctttatg tggcatttat
    30661 cctgctaaaa aaccgggcga tgagctccgc taagcccagc cagccaggag gggagttagc
    30721 cactttcgtg gtggacctgc acgtcaaact gctcacggtg ttgctgtgtt ttgccctgct
    30781 ctttgtgttt gccgcattta cgcgctactt ctacggcatc caggtgcccg tgaagctgat
    30841 cgcttcctgg acggcccggg ctctgggcgg cgtcatgatc ctttactact atctggcaga
    30901 tacatggcta tcaccctggt tgaaccgggg ccacaagcgc caggctgccg tctcccgaac
    30961 taaagtctat gcccggcggc atcccaagac ctacgtcgcc ttcaatctgt ttcagatcgt
    31021 tatggtgttg gcgctggcca gaatctatgt caacctggtg gaatacatct atcatcccat
    31081 cctgacgctg ctcggatcgg ccctcaggct ggagtttgag ctgaagccca tcctgataga
    31141 cggccatctg gccctctggt ggaacgctct gctgactgcc ctggcctttc tgatctcaaa
    31201 cctgggtttt attcctctgg tatggctggc caaactggcc accgatatca cgcatcccat
    31261 ccgtccccca aaaatctatc ctcacactgc ggagaactga cacccattat gcccaaacaa
    31321 agaagaaaaa aaacctccaa acccgccagg aaacgctccc ccaaacattc tggcaaaggt
    31381 atggccttat acatcattgc cggcttgatt tgcgtggcgg cgatctggat gatcacccgt
    31441 cctgtggata gagcctccac cccagtcacg caagagagca ccagcgccag aaccagagac
    31501 aagcggaagc caaaatcccc gccccatcgt cctgcccctc aaacgctcac acctgaggtg
    31561 actgccagcg ccgggctgga tcccgtgatc gtggcggccc tcggagagct gggcatcaaa
    31621 gacggcatct ataaacgccg gaccaaaggt ggcgccgtga tctatgaggc cccgctggac
    31681 aagagtgtct atgatctata tttcgccaac atggtgctga aaggcagggt ggaaaaagct
    31741 ggcggcaagc tggaaagcgc tattgagaag ggacaaagac agagcctccg cttcagctat
    31801 cccgatctgg acaagcactg gaacgtggag ctttattatg actcccagcc ttatgacaaa
    31861 cagacacccg ccaagacctt agctatcgtc gtggatgatt ttggcgagat cggcggcgat
    31921 ctgctggagg gatttttaaa cctgcccaaa gaagtgagct ttgccatctt tcccgggatg
    31981 accaacagcg taagcaccat ggaccaggcc gcggcccagg gccgcgaaac cattatccac
    32041 gtgcccatgg aaccgctggg ctatccacag gtcaatccag gaaaaaacgc cctgctgctg
    32101 caaatgactc aggcggaagt gagcaggatg ctgcatcgct ttatcgatca gatgccccgc
    32161 tgcctgggaa tcaacaacca tatgggcagt ctggccacca ccgatccaga cttgatgggc
    32221 tatgtgatgg aagtattgaa ggccaggaac aagctctttt tggacagccg cacctctaat
    32281 gtttccgtgg cttatcagac cgctcaaaag catcatatcc aggccttccg aaacgatatc
    32341 tttctggatt ctcccgatgt gagcggcacc actctggaaa gcaagatcag gcagctggaa
    32401 caaatgagcg ataagaaacc ccatttgatc gccatcactc actgccacaa ccaggctaag
    32461 ctggtatatc tggtgcagtt tataaaccgg atgcgcaaag ccggttacac gctggtgccc
    32521 ctttccaaaa gtggccgcta tcgggtgccg gagatattgt gaacgctaac gctgtgagat
    32581 gctccgcatg tgagatacct acggtaggtg agatacgaat tcgacaccac tatgctccgt
    32641 tggagttcaa gcggccacga taacggtttg catgacaacg agtttgatgg ccgcttggac
    32701 ttcaaccagt gcaccgaagg acgattcgaa ctccgaagta ctgcttgaag tccaaagccc
    32761 catcagattt agagatagat gtataagctg tccaggggct ttgaactcca agggggtctg
    32821 gaaacgaaga attgtcgtga gcatcctcaa ttgtcaatgc tcaattctca attaaacaag
    32881 tgagttaata tgaatctact ccaatcgatc tttctgggca tcgtgcaggg gctaactgag
    32941 tttctgccgg tgagctcaag cgggcatctg gttttgttgc agcgtttttt gcggctggag
    33001 atgggtgctg atgtggcttt cgaggtgttc ctgcatctgg gaaccctgct ggcagtgctg
    33061 atctatttca ggaagctgat cctggatctg gccttagccc tcttcaagtg gagagacagc
    33121 ccccaaaatc aggcccaccg ctacaaccgc gcgctgatca tgtacatcct ggccgccact
    33181 ctgggcacgg gcctgatcta cctgattttt ggggattggt ttgaatcgct ttacgaccag
    33241 cctctcatcg tggccttcat gctgctcgtc acaggggcca tagttttcgc gtcggactat
    33301 atgaagggtg gctccatccc atccgtttcc atgggtctca cgcgctcggt catcattgga
    33361 ttggtgcagg gcttggccat catccctgga atctcccgtt ccggttccac cattggagct
    33421 tcgctgtttt gtggcctcaa acgtaaggac gcggcgtctt tttcttttct gctgtccatt
    33481 cccgctatcc tggccgccaa ccttaaggag tttaaggtac ttgcctccct gcagccttcg
    33541 cagttcttca tctatctggg gggatttgtc agcgcttttg gagtggcctg gctggtgatc
    33601 tccctactga tcaacatggt ccaaagaggc aatctgaagt actttgccta ttactgctgg
    33661 ggagtgggcg tgctcagcat tatcctgctg gtgagctgat catggcagtt caaagcatta
    33721 acgcgctgga gtttaaaccc tccatgattg gcttggggcg gtgttatttg atcctgggga
    33781 cggatgcctg gctctgtgac agtgtgctgg accccatccg gcgggagctg aaaaatcgct
    33841 taggcgcgga tgtcgtcaat atctacgggg acgaagtcaa ggtcccgcag ctggtggatg
    33901 tattggacac cttctccatc ttttcagctc aaaaactggt gatcctcaga aacgcggaat
    33961 ccctgaaagt ggcagaacag aaagccctgg cagcatatgt gagctcgcct tccgattcgc
    34021 agagcctggt gatcatcgcc acgaaagttg atgcccgtct ggtaggctgg aagagcatca
    34081 aagcaggctg tctggagatc agatgcgatc cgccccgcag tgcttacaac ctcaaaccct
    34141 ggttaaacgg aatgttgagc aaagctggca gaaagatgga agaagccgca attgatctgt
    34201 ttatctccaa ggtagagctg gattttgcca atgccgccaa cgaactgcag aagctgtg
//
LOCUS       DAOSFN010000009        34047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1002_length_34047_cov_9.299041, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000009 DAOSFN010000000
VERSION     DAOSFN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 34047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34047
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1002_length_34047_cov_9.299041"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLO35_01410"
     CDS             <1..939
                     /locus_tag="PLO35_01410"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH78349.1"
                     /translation="SASNPAQVPPDSLFTIRFTPQWHHQSQFAGFYMALRKGFYSNYG
                     LNVIIQDGGSDNPAYEALQSGTTDIITLFLLTAMNKGASSQDMVNLAQFSQRSTLMLV
                     GKKSRGINSVESLQGKKIGLWHSDFRDLSLAFFARNKLKVEVVPIDWTNTLFIHDAVD
                     LINVMYYNEYHSLLMSGIEESDLYKLRFSELGYNIVEDGIYASRAYYDKYPTQCQAFA
                     EASRDGWIYALNHPEEALQVVTAIMKEHYLPSNISHQRWMLDKIGESILAKPTNLGSL
                     LREDFDKAKALMLSQNPALSELTFEEFHPRAVTGLK"
     gene            1001..2845
                     /locus_tag="PLO35_01415"
     CDS             1001..2845
                     /locus_tag="PLO35_01415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOH78350.1"
                     /translation="MIAIVTRRVVSDMMIQNTREYLETLVNARVSEYDGKLLSIAAQG
                     RTLASILEDRLLSVDKLNSHLASILNENPDIASICVAPAPGLDTGIEAGIYMKDENLK
                     LVFNPILPKTYEFRDWYQIAVLKAKPHWGEPWFDEVGIKDLVCSYSLPMSRNGQILGV
                     LRMDTLVKNMQAIKIPARLRQGAYALIISNTGTMITHPADSLLMNYSIFNLAEEYDDT
                     ALMTIGMNMIRGESELVKVSGISFFQDQWIYYAPLPSNGWSIAIVVQDSYIFADRQKL
                     LAIILAFSVLGFLIIALVIYLRTLQIHRPLKEITKAAALIGGGDFDVPLSDPGGIYEI
                     EQLTQGIEAMKLSLKQFIANLSELTIEKEKIQNEVLFASAVQRSLIPDNSIAKKICPG
                     INCHGILKPAGAIGGDLYDFFMIDEHHFCFAIADVVGKGIVAAMTMTMVTTFLRTVSK
                     HQKKPAEMLYSLNSFLSGNELESNLITIMLGIIDMKDGRLLFSNCGHVPWFHRKPDRR
                     LIIHDETHSTALGVFPDITIDDEILLLEPGDQLILMTDGITEATDEAGEFFGHKGVEA
                     ALGELQHPSPDVSAITLLTAVEVFSSGDGSRDDITILALQFEHPHQID"
     gene            2904..2990
                     /locus_tag="PLO35_01420"
     tRNA            2904..2990
                     /locus_tag="PLO35_01420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2938..2940,aa:Leu,seq:caa)
     gene            3167..4201
                     /locus_tag="PLO35_01425"
     CDS             3167..4201
                     /locus_tag="PLO35_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOH78351.1"
                     /translation="MIHRNIFISAALAALMVLPMGLAAISQGSLLFLTFEPGARANGM
                     GRAYSAIADDAYAPWWNPGATAFNRKTQLAGTHTPWLQGAGTGFDDIFYEYLGWNQYF
                     DGIGNLNVHLTLLDAGTQPQTDEAGNYLGDFHSFDFAAAFGYSYEVLPKQLGVGANFK
                     LMYSYLGPGTGNTEDDGSAFAFAFDLGAKYKDLLVPGLDAAMVIQNIGPNVTFVDQSQ
                     SDPLPMTFRIGAAYRVLDTPMNKLIVSAEASKILANDDPLFERFITGWEHIDETILGA
                     GAEYTYLDLLSLRGGYFVDSAGSIVGPSFGAGVQYKFDNRYRLTADFAMIEAGDLTDY
                     NKVFSIGFEF"
     gene            4214..7402
                     /locus_tag="PLO35_01430"
     CDS             4214..7402
                     /locus_tag="PLO35_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78352.1"
                     /translation="MKKIFTLRIADRLPEGLAFRVVRITFLLLWLSLSCGLSALGGET
                     MFLRKGWQHNSLKQHPYANQLTKSTDTARVARGPNYDRLLVILVEFQPEAVDDPNTTG
                     DGRFQSQPDPSYLYSIGSAPHDREYFMANLEALRYYYLAVSCGSYDLSYDVYPDDGSK
                     LVLPHSMGFYNPPGASSTLFTERMEEYFAHSFSLADQQFPQIDFSNYGHYMIIHAGSD
                     WQHDILGDTPSDIPSFFIRVTDDKAVSVDGGTHLIKHACNVPATISQDFSSETTDGVT
                     VHSGYGALNAVIAHEFGHSLGLVDLYNVYNFRPMVGVFDIMDSGGSGILVDQLNDGSL
                     VMVEGILPALPGAFSRTLLFEDWYRANGYLKDVRDVELFTPLPLAASSMKQTGPVSPN
                     IYKVPITGEEYFLVENRSVDPDNDGATAVFGALDGRVVLYPTPLNDPQNLPSYEYDYL
                     LPSFQRPNADAVGGGILVWHVNNDVIYNQGVVYQDGSWASNFNNNTVNTDYHNRGVQV
                     VEADGLADIGNDWSWYWTGTQYEYFHKFRPVLDSGGYFQNWSQSSWKPMLGPNSDPAL
                     TSFAGNPSLYWLAGIGNPSAIMSFELRSGFFEGSDTIALENGAVSAPLIDTSFDHAQL
                     PVFGPTGIRLFSTGSSPWSDLMGEFPYTDGTGNYPVTRSEQTPNSYHELVLSMDDRLR
                     IIEFGNDALDMTDIDLGAPPTLQALAYNDKVYTATSDAVFQIGNNAIQGRAEIADARK
                     LAIAGNELLVLTKSGIVFLDLSNLNETRRYTLSGEFGDYEPLVFLNDETNQTIAVLTN
                     NQGDIWSCRNNEVARIFSNNSTALPTQLGITSISGQSPIIFFGLGDQAYALRPDGTNL
                     SGFPYHTDTYDYEAHRHVYSVTLSGDTFMQIPVEAEGYHAVSILPSSVPQNALSLPAR
                     LDNDRFYYDGANGILYWYFIDSQANLCYQWMSVEENPVLWAGPRNDGTGQFMGAESHS
                     ASTQTALSAYVYPNPVNSSPLRVRAERLDSNPVELKVYDVAGKLVLDKSFAANPLDSR
                     DFQLDHRLSSGVYFLTVGNGSRHTRIKFAVIN"
     gene            7425..8474
                     /locus_tag="PLO35_01435"
     CDS             7425..8474
                     /locus_tag="PLO35_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78353.1"
                     /translation="MKRFILLTITLMMMISLFGQSDIDDLIFGPEKQDQAPAKDPKLA
                     KPTDDDFIKVNFAKKDARLAMLFSALLPGAGQYYADKSALSTWIFPIVEVGLIGGIIY
                     YNVSGDKRTDDFEKYATGEIITQTFNYTVDGVDYSYTYQGPRYQRSYQNLTQNVLMSF
                     FPSDIYDSNFFRLDATNTQHFYEDIGKYNKYIFGWADWFYNFATDPMTGNFVLDNPNY
                     YEGSFPGLNLNTVWIWADTQYDYSRRWTANVQIDDFLNGLSGNHISPGTTMASPMRQE
                     YIQMRKDANSQFSIARIMTLALAFNHLASAVDAVMVTNKVNKTYITQNNLQLKYYTAF
                     RDGRVSPSLGFSWSF"
     gene            8476..9129
                     /locus_tag="PLO35_01440"
     CDS             8476..9129
                     /locus_tag="PLO35_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78354.1"
                     /translation="MRIVLLVLLTTLLAMPLIALPRIAYIGMSAALPGSAELALGKPT
                     RGGIMIAADLLSWLAWSGYKDEALDLEDSFKRYAQAYAGVPSNSNDRYYQHIQQYYSS
                     DEFNSFQEMMARNFYLIYNYDPEGFSEYLAANLYADEEGWQWQSPEHHQKFRDLRKQR
                     QKAKMYQNLALGALLLNRGISIVDALFLSGKKSEAELPVYFSVSPERALILNYRFEF"
     gene            9139..9783
                     /locus_tag="PLO35_01445"
     CDS             9139..9783
                     /locus_tag="PLO35_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78355.1"
                     /translation="MRRLLIIVLLLGSVGLTAQSRGLKFLKSLVVPGLSQVSEGRNYG
                     YAMMVSEISIIGGMMYLNSEEKLKARESYEYALKYAHIRPGGYEESYFRDLSRYNSGG
                     FDADGFNARVRRDAMNLYPHDPVAQQQYIDSHAYPEDMYWNWDSAQKRASYSKIRIKT
                     QDLRDFGQIAVGVLLVNHLISGIDVLRYDSEGRRSQVYMSMKGKQPILNLSVSF"
     gene            complement(9915..>10193)
                     /locus_tag="PLO35_01450"
     CDS             complement(9915..>10193)
                     /locus_tag="PLO35_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78356.1"
                     /translation="YLTKQSWDFPGEEVLVEDKYLVSTDVVRGKLPCPFGHPGVHRKA
                     ITTLFNKSMGLSVRWTSLSIHLIDAHSFFEGKGSPFRLDPETLIKAIF"
     assembly_gap    10194..10293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10294..11333)
                     /locus_tag="PLO35_01455"
     CDS             complement(<10294..11333)
                     /locus_tag="PLO35_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOH78357.1"
                     /translation="MLLTILKDIGILTAISAVLGILLVIAQKWLSNYGECKIQINGKR
                     DVTVKGGSSLLNSLADKQIFIPSACGGRGSCGACKCQVREGGGPLLPTERPLLSKSEM
                     ATNVRLACQVKVKSDIAIQIPDAIFNIRKYKTSISEIVDYTYDIKGVTFKLQEGETID
                     FLAGQYVQVNTKPYGKMRQSVSRAYSISSRPEDSDRFQLIIRLVPEGICTTWVHQHLQ
                     VGDEVEVTGPYGDFFIRDTDADIIFVAGGSGKAPIKSMLEHLKVTGCDRKMTYFFGAR
                     TTKDLYLTDEMKAFEQVFPAFSYEPVLSHMEPDDNWQGKTGFVMPYFKDVIRDPLKTE
                     AYLCGSPGMINAV"
     gene            complement(11346..11933)
                     /locus_tag="PLO35_01460"
     CDS             complement(11346..11933)
                     /locus_tag="PLO35_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOH78358.1"
                     /translation="MDISAFVLFWAAIFTSNILLTNFLGMCSFLSVSREVESSAGLGI
                     AVIFVLTATAGLNWLVYHYVLVPFGIMYLQFIVFIIVIAAFVQLLELVVERYAPALYY
                     SLGIFLPLITVNCAIFGVSLFLVIRDYNFLQSIAFGAGSGIGWLLAILMLAGIRAKLK
                     EKLVPAGLQGAGISLIITGIMALAFIGFSGIVQIQ"
     gene            complement(11933..12529)
                     /locus_tag="PLO35_01465"
     CDS             complement(11933..12529)
                     /locus_tag="PLO35_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOH78359.1"
                     /translation="MTKKDIFIVSAFKENSVWKQILGICSSLAVTNLMLNSLIMGLGV
                     IFSLALANYTISLIRSYTPRSVRMMAQTLIIGAYVIIVDILLKAWMPEISKQLGPYVG
                     LIITNCILMGRAEAFASQNKPVDAMIDGIGAGVGYTLVLLLIAFVRELFGFGTLFGFR
                     VMGAGWTNWSIMVMAPSAFFILALMIWAINTYYYKGAK"
     gene            complement(12526..13197)
                     /locus_tag="PLO35_01470"
     CDS             complement(12526..13197)
                     /locus_tag="PLO35_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOH78360.1"
                     /translation="MSKERVPFIETMSYPVIFMIMVSLIFVGILAVLFRFSENRIETY
                     KRESYQKLVLQLCASSISKATGISAADITSRYPQSYDQYITKSPLKGEERDSWAVSIN
                     DSVIVRCVDIPGKGLWGSMRGLVSLSPDLTQIRDLAIYEQMETPGLGARISEEWFQAQ
                     FRYRTVITDGKFMDLELIPEAQAANPNQVNQVTGATITSAAVLNMLSTELQKLHDQNH
                     PGGQR"
     gene            complement(13194..14132)
                     /locus_tag="PLO35_01475"
     CDS             complement(13194..14132)
                     /locus_tag="PLO35_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOH78361.1"
                     /translation="MFERLMKQAVMNRVLYALAPLLIFAVYLFGWRVLAVVLVANIAA
                     YITEYLFIRTKKGGKVTMAAFVTATLVAMTLPPTIPLWMSAVAAVVSIAFGKMVFGGF
                     GTNPFNPAILGRTFVYVAFPQQMTIAWLKPFLLSDFPGGLASWTARAGMETSATIMGH
                     YRLKEELIYSFKDAFLGFVPGSIGETSALLIILAGIYLVLTKTAKWQPMVATVLSLLV
                     FGFIFYPGENPVYFLASGGALFGVVYMTTDPVSQAKGKLAIWIYGVLIGFLTVFIRRY
                     SLFAEGFMFALLLANSFMPIIEYYLDKRTKKKGAKA"
     gene            complement(14125..14496)
                     /locus_tag="PLO35_01480"
     CDS             complement(14125..14496)
                     /locus_tag="PLO35_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78362.1"
                     /translation="MEALPDCDLADTRSASLREAIPALSESFASSPEDTNTCILQLRI
                     EKQEQRLAGFLIESLDGVAIHSHGKDEHHLRLICNRSRDPELRTFLAAWQRSQSHTSH
                     ATQDLIALSDASNDKGDTHHV"
     gene            complement(14531..15109)
                     /locus_tag="PLO35_01485"
     CDS             complement(14531..15109)
                     /locus_tag="PLO35_01485"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOH78363.1"
                     /translation="MDRNKLRKAGYLALIGAGVIIVTAFLTGQVIMPLIFAKPSNIEV
                     PDLIGKNISTAKRLLKEAGLHAVVRDSVWSDSAKIESVIQQHPEPGTLAKLEGTVYLV
                     ICKGSQIVAVPGVVGRSYEEAFITLRNSGLRASVADSLYSQSYPANTVIRTSPPNGSR
                     VDKNAMVRLFLSRGPEPVADTLSTPEIDPGTY"
     gene            complement(15114..16427)
                     /gene="rsmB"
                     /locus_tag="PLO35_01490"
     CDS             complement(15114..16427)
                     /gene="rsmB"
                     /locus_tag="PLO35_01490"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOH78364.1"
                     /translation="MLRAEAYQTILKVFKEGEFSDTLLHQRSKRIKQAGENLSLFYSL
                     VKGVIKMRGHLDFIISQHTDAIKYASTDLKIKILLYVGLYQLIYLDSIPSHAAVNETV
                     ELAKQLFNPNVADFVNAILRAWQRDQSVSYPDDPISKAAFEHSYPPDLVKRWVELWGL
                     ENAEYLMLYFNENPILHIRVNSTATTPEKLMQYYAKRGVELKPTGYGAMMLSTDQADQ
                     VLEDVAFSEGYFSVQDSSAAMIVDLLDPRKDDNILDLFAAPGGKCTYIAEKMENSGEV
                     IAVDKIPNKMKLLKQAADRLQLSNIRLVTADAFKYGPVAPAYDKVLVDAPCSGWGVFG
                     RKADLRWQAHSNISELIKIQERALDHAANFVRPEGFMIYSTCTMNPDENEHQIQRFLA
                     RNTRFVLSPPGALIPDNCVRDGMFRSIPFIHHMDGAFGAKLQRIR"
     gene            complement(16429..17034)
                     /gene="ruvA"
                     /locus_tag="PLO35_01495"
     CDS             complement(16429..17034)
                     /gene="ruvA"
                     /locus_tag="PLO35_01495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOH78365.1"
                     /translation="MIAYIKGKLITKNPVSVTIETSSGLAFDLKVPISTFEKLPAAGS
                     SCQLFTHLHIAQDDIRLFGFHALAERELFLILTSVSGIGPKIALSILSTMPISTFVRA
                     IENGEEGLITRVPGLGKKSAQRLIVELRGGFAALGDHFKAEDHILQGSVVDEVESALV
                     TLGFNPKDIRRELALMPEEAKSMGSEQLIKETIKRLYQRSR"
     gene            complement(17031..17510)
                     /gene="ruvC"
                     /locus_tag="PLO35_01500"
     CDS             complement(17031..17510)
                     /gene="ruvC"
                     /locus_tag="PLO35_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOH78366.1"
                     /translation="MVLIGIDPGSRVCGYGLLETQGRVITAAGCDVVDISSYTTLPER
                     LAALFARVEAVLAEFKPEIAVVESMFYHKNVKSMLTLSQARGVILLAFARYGLPVFEY
                     SPREVKKAVVGNGGASKQQVRYMVEQLFKLKGSDKPDDAYDALAIAMCHFNRIRFLS"
     gene            complement(17510..18259)
                     /locus_tag="PLO35_01505"
     CDS             complement(17510..18259)
                     /locus_tag="PLO35_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH78367.1"
                     /translation="MSGHNKWSTIKHKKAATDAKRGKIYTRIVKEIILAAKSGGGDAD
                     MNPRLRTAINAAKAANMPRDNIERAIKRGTGEIEGAAYEEITYEGYGHNGVGIVVDVM
                     TDNKNRTVSELRHTFSKYGGNLGETGAVSWNFEQKGFFNVPAVGLDEDEFMMQALEAG
                     AEDIELNDENFDVFTSPSDFHTVLANFESLGFPVLNAELTKIPKNTINADDVAGKLLR
                     LIEMLEDLDDVQKVYANFELSDEVMESLNRE"
     gene            complement(18414..20468)
                     /gene="fusA"
                     /locus_tag="PLO35_01510"
     CDS             complement(18414..20468)
                     /gene="fusA"
                     /locus_tag="PLO35_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH78368.1"
                     /translation="MKEYDLKKIRNLLLMGSGGAGKTSLAEQIFFLTHATNRIGKIDE
                     GNTVMDFDAEETAKKMSLSLSIGFVNYNDHKINILDAPGTPDFVADAIVAIPAVENVV
                     LVANAAAGFEVGLELAIEQLEHKKVGKIVVVNRMDNEHADFQKTLDSIYENSGLKATP
                     LMLPIGKEGSFEGVIDVISMKAIRPAGVSDIPAELHDLAEEARLHLMEAVAETDEELL
                     NSFLENMELSQEQMMPGLKKAIASGEIIPAFACSANTGVGVNAFLDATLAYLPSPVES
                     TALEVISGEERKSFMVSPDGELLGYVFKLYSDPNMGEYAYVRIFSGNLKAGTEFYIPE
                     KDAKDKAGNMYYMLGKTRHDTQEIRAGEIGALVKLKNARALNSITALNSRLALPMPEL
                     PTATYWQMIRAANQSDEDKIGSSLQRVIAEDPSIRFELNQETHENVISGMGEAQMTLV
                     LKKLKNRYKVDAMMKEPRIPYKETIIGSAESQYRHKKQSGGRGQYGEVYFRIKPVERG
                     EGFHFINAIVGGVIPSNFIPAIEKGIVETMEKGIIAGYQVVDISVEVYYGSYHDVDSS
                     EMAFKIASSMALKEGFKKCKPILLEPIHDLVIIVPSEYMGDVMGDISTRRGRIMGMEQ
                     RGKKQYLNAQMPVAEMYLYFPALKSFTQGRGRFTQKFSHYEKVPEDITQKVVAAWQDS
                     EA"
     gene            complement(20588..22312)
                     /locus_tag="PLO35_01515"
     CDS             complement(20588..22312)
                     /locus_tag="PLO35_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOH78369.1"
                     /translation="MGPITEHNLTLFLIQFGLLLGICKIAGYFFEKLKQSSVTAELLV
                     GIILGPTILGKLAPGIHAGLFPDNQVQRSMLETIAWIGNFFLLMETGLEVNFSRIWRQ
                     KTQALIISFSDLIIPVLVSFVPMLLLPDRYLVDPEHKLLFALFMSVIMTISALPVAIR
                     GLRDMNILKTDVGFLIISALTINDIVGWVVFTIILGLFARGSLELGYIGQLMLLTLGF
                     TVISLTIVKSVVDKSVSFLHKKVDQSSGLKITFIILLGILFGALTLGIGIHALFGFFI
                     AGVVLGEATHITEKDRFVVNRLVYSIFVPIFFASIGLHLDFMANFDLFLTLLFLVVGV
                     AGRFLGAWLGAKWSRQDKSDILTIAIAHTPGGQMHIVVAMLAYSLKLINTEVLVAIIA
                     SAVLSTIIFGPWLSYAVNKIRNQLFSVIFKPRHVLINKLTADRTQAIEALISLAAQDL
                     GIPKTALAREVELREAQMSTALGKGIAVPHARIDSVEQSYVYALMAPRGIEWDSPDGD
                     PVQLALLLITPIDNPKAQIQILQSLALGFRNKDKLEELLHEPDEGQLFSGIKQCLNSC
                     DQCQLREA"
     gene            complement(22672..23841)
                     /locus_tag="PLO35_01520"
     CDS             complement(22672..23841)
                     /locus_tag="PLO35_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78370.1"
                     /translation="MTTKRMILVLIPVLMAASLFAYDFKWDGMFRTRAAAYNDDMGTP
                     GAHIDNRLQLGLKSELVPGLTLRAKFEIGQNGGDANLVWGGDGGGLATNGINVKTNEA
                     YIDYRIEAIKANVRVGQQYWADHRSLILDDTFSGVTFHTSTPDGINGMLGFGKYAEGN
                     YYNRYDDMQGIIFSLDTAKPVEAGLQGYLTWSRVRPIATEDAIQLNYISVMPYASMNI
                     DPITVDGVLFVQSNDSFSFVGGDLEKESDLSFGVALKAGVDVQPLEVNADILYISENG
                     LMTLSGYYQNGLYLLGIGEHHDGLGLWWGATGNPDMYLGAALQAKFEAMKGVKVFGAG
                     GMVLDTGLELNGGLEVELVPNLLHLAAFAAYSIMDEDVSDQDNYALGTTIKLAFD"
     gene            complement(23904..24998)
                     /locus_tag="PLO35_01525"
     CDS             complement(23904..24998)
                     /locus_tag="PLO35_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOH78371.1"
                     /translation="MTKLPVSTLQEFMTEVLTRIGVPASDAQICSEILIASDLRGIES
                     HGIGRLKMYYDRIRAGIQDPITRIDVVKDRMATAVWDGNHGMGHVISKSAMQTAIDKA
                     AQFGLGAVAVRNSTHYGICGFYTEMATRQDMIGLTFTNARPSVCPTHGVQPLLGTNPI
                     CFGAPTQLGFPFIYDAATSISQRGKIEQYAREGKDTPSGWAIDLDGNAHTRTPELLVD
                     LVKQTASLLPIGGTDEVSGSHKGYGLSTMVEILCAALANGSFLNGLLGQDADGKPAPY
                     RLGHFFMAINIAFFTELDEFKSTSSEICRQLQNSQKYPGQQRIWVAGEKEHEKRLQVL
                     AEGVPVIPKLQQDIITMQRELGFEMLKFVD"
     gene            complement(25026..26204)
                     /locus_tag="PLO35_01530"
     CDS             complement(25026..26204)
                     /locus_tag="PLO35_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOH78372.1"
                     /translation="MYKETYHFNVERYMNPERFNKLRDYATQLQTPCLVIDLDLVSKK
                     YRELQTCLPGAAIYYAVKANPMPEVLKLLDSMGSHFDVASRYELDLMLGLGISPERLS
                     YGNTIKKRRDIAYFYEKGVRLFATDSEPDLLNIAQEAPGAKVFFRILTEGTGADWPLS
                     RKFGAHSDMLYYLIIQAQDLGVQPWGVSFHVGSQQRDIGQWDDAIARCKYLFDAVAEE
                     GIELKMINIGGGFPANYVDPTFDVSDYTNEIIRFIKEDFGDHLPQIILEPGRSLVADA
                     GLLVSEIVNISRKSKNNIYQWVFLDVGKFGGLIETIDESIKFPIFFDREGQADEIILA
                     GPTCDSMDILYEHYKYHMPETAQPGDRVYIFTTGAYTQSYSSINFNGFPPLRAHAFCG
                     "
     gene            complement(26339..26614)
                     /locus_tag="PLO35_01535"
     CDS             complement(26339..26614)
                     /locus_tag="PLO35_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOH78373.1"
                     /translation="MSRDDLIKKIAGDAGISQKVAGLALAAVLEGITLALKKGDRVSL
                     VGFGSFSVAHRKARNGINPKTRKALKIPARKVPIFKAGKKLKEAVKK"
     gene            26912..28096
                     /locus_tag="PLO35_01540"
     CDS             26912..28096
                     /locus_tag="PLO35_01540"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78374.1"
                     /translation="MKKHLFLYLAAATIILILISSCAKTKTEVETVAAPPKPIDLAHA
                     ATKAGEEAFQNQLLDEAKLKFKEAIVQFEAAAPTAAATDSIDVNIERMHINIAKVNMD
                     LATESTAAQMHSEAIEQLQEALDSFKKIQPITIPATEKDEIIKSLYIRLAYANQSAGS
                     YEDALLAYDEVLKMEPGNEEILNNKYVILNDNIKDETRAFQVLKDYAQASNDYRAYLI
                     LGTRYNEKKNVAEATKYYEQALTLNKSADVLTRVSDFYRSNSNWAASNKVLDQLVATK
                     PDNATLAAVYRLMGDNYSKLKNNAKKADAWEKSLALEPNGEIALAVAKHYYDAKNYNK
                     AVTYATQAINQDSGNAAAYLLRGDCYYKLKKNAQAKADLQRIQNDPQHGSTAQKLLKA
                     IK"
     gene            28412..28858
                     /gene="umuD"
                     /locus_tag="PLO35_01545"
     CDS             28412..28858
                     /gene="umuD"
                     /locus_tag="PLO35_01545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOH78375.1"
                     /translation="MNKLKTDGTIKAIYGFRKDLKLSRPLVGPRVPAGFPSPAQDYME
                     GSLDLNEYLVSHPASTFFVRVEGFSMIKAGIFPDDILVVDRSLEPASGRIIVAIYDGE
                     LTVKRLKIECNHYWLEPENDEFEPICVEEETDFLVWGVVCWVIHKV"
     gene            28859..32131
                     /locus_tag="PLO35_01550"
     CDS             28859..32131
                     /locus_tag="PLO35_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78376.1"
                     /translation="MRSLRFVLLILLLNLIQGYVYGETSLLQERLIALVPGIGTYDLS
                     SGPIIVGSEQISTGDVILLRGIDYRIDYRKGQLTILKDLTAVYISVSFILVPEDLNNS
                     RFLYETKAETDSVFRSIQATKRSSWWQDDGKLLINGSKTLAVSFSDEQAFELKQSLYV
                     NLSGELAEGVNISAQLSDSQSKLSPEGDSRELSSLDQVFIKVYGKRWELAMGDIELKY
                     LGSRYMDYSSRFEGINATIGADSYLQAAYSAGGGKPSRIDISVIDGKQGPYYLNPGGQ
                     PGSLIVVPGSENVYRNGTRLERGADYNIDYSEGTLMFRILVYATDTISAWFSYSDENY
                     PRHSILSSSNLSITPRWQVSHRVISASDAKDNPLLFQFSPADLDSLQAAGDSDAWGSG
                     ATLVEAGQGLYRLLSTPGGISYYQYAESDSLANYLVIFSYVGPQLGDYEQFSSGKYRY
                     LGPGLGEWLPQKRLVSPNSSVNASLRSAYSYGSWDLGAEAVLAVNDANTLSGLDDDDN
                     QGGYLYSWLNYKNARSRWRPELLLDYEKRWAHTVLLSDFSASISDYDLGSAVVPDSLE
                     LDSYGLKLGVNLGKSFTSELSVRHLRSDDDLEQSILRFRSSSPAWGLFPRLDIRSTLA
                     RTSASLEGLGTGDSNYLFIDGAWSAAWLKWGGLINLQENADISQDFKTSFLRLNPYGE
                     IGNSKSVQTRLAFSADQNKIVDHSVSNSQSSQTWSFKQLVNTTNHNLNLDYNHRRLNL
                     AADSTRTSYDLINLRANDSFLKRAITLISNYQLNQTEFFPRIRELQYVGSGLGVYDST
                     GVAVPEGDWDYEYITSSTGTMSSERNAQTSIYLKPGNLWENDILRRINTDIVISGTEQ
                     QEGRQTDLLSYLFWPGRVFNLENTIYGRQSFQQNIWLELRRNKIIAQLVLDLERSLDN
                     RYQDLSRTYRRSGALKLDFNRVGPYSFSITAENKRDSDSRYHSDVNNFALSSFAQREL
                     GKNSILRLDLRYFEEQGSSQLNSDSYGLYGVALSPSFRSVWGSKGRLSGGLNLQYNQR
                     EGDDLLSFLPEKRGGAIIGLNLSAIYRLNAFSTGNIDYTATSYPGEDISHTLRIEFKA
                     EL"
     gene            32132..32923
                     /locus_tag="PLO35_01555"
     CDS             32132..32923
                     /locus_tag="PLO35_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOH78377.1"
                     /translation="MDTGIITANGTLDYLVVLPFIFLAGLIDSIAGGGGLISLPAYWS
                     IGIPPHTALGTNKFSSCCGTVFSTARYFHAGMIDIPVAVWSAVAALLGSWLGARVALM
                     VSAAFLNYMLVILIPGMTLLTLLRKDIGQKDHSARLKLGYRIGLGLVAGLVIGFYDGF
                     FGPGTGTFLILIYAVLLKYDFVVANANTKVVNLASNVAAVVTFAVAGKILYAIAIPGA
                     LCGIAGNLLGARLVIKKGNRLIRVIFILALILLLGRVLSNLLARL"
     gene            33052..33738
                     /locus_tag="PLO35_01560"
     CDS             33052..33738
                     /locus_tag="PLO35_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78378.1"
                     /translation="MRKIAFLMIVLLMACSAALAQEQVPKQTLWAKQFPATDLNLDVR
                     YSTELKRLYIQVDTQVAKTAIDNFALNGFFLPRDARIVGLWINGKPSRILFVGKLQPG
                     NFNPAITSADLLSDNSPARYHVMALNGYAEYPDMVKVNLSYYLDMPDFQPNSLNQMYT
                     LLKPDKFWYPRNINVPTNVKIKVVTTTYMRLMLGDALVPFTDKDYMREHVASFRDDPA
                     EPLSFRLTRE"
     gene            33742..33817
                     /locus_tag="PLO35_01565"
     tRNA            33742..33817
                     /locus_tag="PLO35_01565"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33775..33777,aa:Thr,seq:cgt)
BASE COUNT         8346 a         8758 c         8496 g         8347 t
ORIGIN      
        1 tcagcgtcca acccggctca ggtgcccccg gattctctct ttacgattag attcaccccg
       61 caatggcatc atcaatcgca gtttgccggt ttttatatgg ctttgagaaa gggtttttac
      121 agcaattacg gtctgaatgt gatcatccag gacgggggga gtgacaatcc agcttatgaa
      181 gccctgcaga gtggcaccac ggatataatc accctttttc tgcttacggc catgaacaaa
      241 ggtgcctcaa gccaggatat ggtgaatctg gcgcagtttt cgcaacgctc cacgctaatg
      301 ctggtgggca aaaaaagcag gggaataaac tcggttgaat cccttcaggg caaaaagatt
      361 gggctgtggc actcagattt tcgggatcta agcctggcgt tttttgccag gaacaaattg
      421 aaggtggaag tggttcccat cgattggacc aatacgctgt tcatccacga tgccgtcgat
      481 ctcatcaatg taatgtacta caatgaatat cacagtctgt tgatgtcggg gattgaagaa
      541 tccgatcttt acaagcttag attcagcgag ctgggataca atattgtgga ggacgggatt
      601 tatgcctcca gggcctacta tgacaagtat cccacacaat gtcaggcttt tgctgaggcc
      661 agcagagacg gctggatcta cgctctcaac catcccgaag aggcccttca ggtggtgacc
      721 gcgatcatga aagagcatta cctccccagc aatatttctc atcaacgctg gatgctggat
      781 aaaatcgggg aatcgatcct cgccaaaccc accaatctgg ggtctctgct ccgagaagat
      841 tttgacaaag ccaaagcttt gatgttatcc caaaatcctg ccttgtctga actcactttt
      901 gaggaatttc atccccgtgc ggttaccggg cttaaataac aagtcgtttg cctaccggtt
      961 gatatcattc gtcctggtag tgatgatcct ggtttactcc ttgatagcca ttgtcacccg
     1021 cagagtagta tccgatatga tgatccaaaa cacccgggag tatctcgaaa ccttggtaaa
     1081 cgcccgcgtt tctgaatatg atgggaaact gctcagtatc gcggcgcagg gcaggaccct
     1141 ggcctccatc cttgaagaca ggctcctgtc tgtcgacaag ctcaattcac accttgccag
     1201 tatcctgaac gagaatcctg atattgcatc tatctgcgtg gctcccgctc caggtttaga
     1261 cacgggtatt gaagccggca tctacatgaa ggatgaaaac ctcaaactgg tatttaatcc
     1321 aattcttccc aagacttacg aattcagaga ctggtatcag attgcggtat tgaaagccaa
     1381 gccgcactgg ggcgaaccct ggtttgatga agtgggcata aaagatctgg tctgttctta
     1441 ctccttgcct atgagcagaa atggtcagat tcttggtgtt ctcaggatgg acacattagt
     1501 taaaaacatg caggccatca agatacctgc cagattgaga cagggtgcct atgccttaat
     1561 tatatccaat accggcacca tgatcaccca tccagcagac tcattgctga tgaactacag
     1621 catcttcaat ctggctgaag aatatgacga tacagctctg atgacgatcg gtatgaacat
     1681 gatcagaggc gaaagtgaac tcgttaaagt aagcgggatt tcatttttcc aggaccagtg
     1741 gatctactat gctcccctgc cttccaatgg atggtccatc gctatagtcg tccaggattc
     1801 ttatatcttt gccgatcggc aaaagcttct ggcgatcata ttggcttttt ccgtgctggg
     1861 ctttttgatt atagctctgg tgatctatct ccgcaccctg cagatccatc gtcctctcaa
     1921 agagatcacc aaagccgccg cgctcatagg tggcggtgat ttcgatgttc cgctttccga
     1981 tccgggcggt atttatgaga tcgaacagct cactcagggg atcgaagcca tgaaactgtc
     2041 actcaagcag ttcatcgcca atttgagcga actcactatc gaaaaggaaa agatccaaaa
     2101 cgaagttctc tttgcgtcgg cggtacaacg cagcctgata ccagacaact cgatcgccaa
     2161 aaagatctgc ccgggtatca actgccacgg gatcctcaaa cccgcgggcg ctattggtgg
     2221 agatctttac gacttcttca tgatcgatga gcatcatttc tgcttcgcga tcgcggacgt
     2281 ggtgggcaaa ggaattgtcg ccgccatgac catgactatg gtgaccacct tcctgagaac
     2341 agtctcgaag catcaaaaga aaccggcgga aatgctctat tccttgaaca gctttctgag
     2401 cggtaacgag ttggagtcaa atctcattac catcatgctg ggcattatcg acatgaagga
     2461 tggacggctt ctcttttcga actgcggtca cgttccctgg tttcacagaa agccagacag
     2521 gcgcttgata attcatgatg agactcactc taccgccttg ggagtgtttc ccgatataac
     2581 cattgatgac gaaatcctgc tgcttgagcc aggagaccag ctgatcctga tgacagacgg
     2641 gatcactgag gcaactgatg aagcgggtga gttctttggc cacaagggtg tggaagcagc
     2701 tcttggcgaa ttgcagcacc ccagtccgga cgtctcggca atcacacttt tgaccgcggt
     2761 ggaagttttt agcagcggtg atggctcccg ggacgacatc accattctgg ccctacaatt
     2821 tgaacacccc catcagatag actagttaca gggctttttt gcttgacaag gaacaggggg
     2881 ataaaatcct gaatccaatc agtgccgaag tggtgaaata ggtagacgca gtggactcaa
     2941 aatccaccgg gcttaggccc atgccggttc gattccggcc ttcggcacca agagcaaaaa
     3001 ggagtattaa ccgatatttg cgctgtgaga gttgcatttt ctatggaaag tcacgcgcac
     3061 atgcagtatg tggttttttt tccctctgta gtgtgtatat tatccgtaaa cattaatagg
     3121 ggttatttct attccccata agaaatccta attattagga ggaaacatga tacatcgtaa
     3181 tatattcatc tcggccgccc ttgccgctct gatggtcctg cccatgggac tggcagcaat
     3241 ctcacaggga tcgctgctgt ttctcacttt tgagcctggc gcccgcgcca acgggatggg
     3301 acgcgcctac tctgccattg cggatgacgc atatgctccc tggtggaatc caggtgccac
     3361 agctttcaac cgtaaaaccc agctcgctgg aacgcacact ccctggctgc aaggcgcggg
     3421 gactggattt gacgatatct tttatgaata tctgggctgg aaccaatact tcgacgggat
     3481 cggcaatctg aacgtgcacc tcacgctttt ggatgccggc acccaacccc agactgacga
     3541 agccggcaac tatcttggcg attttcatag ctttgacttc gcggcggcgt ttggatacag
     3601 ctatgaagtc cttcccaagc aattgggggt gggcgctaat ttcaagttga tgtacagcta
     3661 tcttggaccc ggcaccggca ataccgaaga tgatggcagc gcttttgctt ttgcctttga
     3721 tctgggcgcc aagtataaag acttgctggt acccggactg gatgccgcca tggtgattca
     3781 aaacatcggc cccaacgtga catttgtgga tcaatcgcaa tcagatcccc tcccaatgac
     3841 attcagaatc ggtgcggctt accgtgtcct cgatacaccc atgaacaaat tgattgtctc
     3901 cgctgaagcc agtaaaattc tggccaacga cgatcccttg ttcgagcgct tcatcacagg
     3961 ctgggaacat atcgacgaga ccattctggg agctggagcg gaatacacct atcttgatct
     4021 gctttcactg cgcggaggtt attttgtgga ttccgccggc agtatcgttg gccccagctt
     4081 tggcgccgga gtgcagtata aatttgacaa ccgttaccgt ctcaccgctg attttgccat
     4141 gatcgaagcc ggagatctga cagattataa caaagtgttt tcgataggct ttgagttcta
     4201 aataatctca ttgatgaaga agatattcac cctccggata gctgacaggc ttccggaggg
     4261 attggcattc agggtggtgc gaatcacctt tctgttgctg tggctgtctc ttagctgtgg
     4321 cctgtccgct ctgggcggtg agacgatgtt cctcagaaag ggttggcagc ataatagcct
     4381 caagcaacat ccctacgcca atcagttgac caaatcaaca gataccgcca gagttgcacg
     4441 cggtcccaac tacgatcggt tgctggtgat cctggtggag tttcaacctg aagcagttga
     4501 cgaccccaac acaactggtg atggcagatt tcaatctcag cctgatccat cctatcttta
     4561 cagtattggc agcgctcctc acgaccggga atactttatg gccaacctgg aagctctgag
     4621 atattactat ctggcagtat cctgtggctc atatgacctg agctatgatg tgtatccaga
     4681 cgacggcagc aagcttgtcc tccctcattc catgggtttt tacaaccctc ccggagccag
     4741 ttccacgctc tttaccgaac ggatggaaga gtactttgct catagctttt ctctggcaga
     4801 ccagcagttc cctcagattg atttctccaa ttacggccat tacatgatca ttcatgccgg
     4861 ctcggactgg cagcatgata ttttgggaga cacgccatcg gatattccct ccttcttcat
     4921 cagagttacc gatgataaag ccgtcagtgt ggatggcgga actcatctga tcaaacacgc
     4981 ctgcaacgta cccgccacca tatctcagga tttcagctcc gagactacgg acggagtgac
     5041 agtgcacagc ggctacggag ccctaaacgc ggtgatcgct catgagtttg gccactcgtt
     5101 agggctggtg gacctctata atgtgtataa tttccgccct atggtaggtg tgtttgacat
     5161 catggacagc gggggctcag ggatattggt ggaccagttg aatgatggct cactggtgat
     5221 ggtggaaggc atccttcctg ctttgccagg cgctttttcc aggaccttgc tcttcgagga
     5281 ctggtaccgt gccaatggtt atctcaagga cgttcgggac gtggaacttt tcactcctct
     5341 tcccctggct gcctccagca tgaagcaaac tggccctgtc agccccaata tctataaggt
     5401 cccaatcacc ggggaagagt acttcctggt ggaaaaccgc agtgtcgatc ccgacaacga
     5461 cggtgccacg gctgtttttg gagctctgga cggcagggtg gtcctctacc ctactccgtt
     5521 aaacgatccc caaaacttgc catcttatga atatgactat ttactgccat ctttccagcg
     5581 tcccaacgca gacgcggtgg gtggaggaat actggtttgg cacgtcaaca acgatgtgat
     5641 ttataatcaa ggggttgtct atcaggatgg cagttgggcc agcaatttta acaacaatac
     5701 cgtcaacaca gattatcaca atcggggcgt tcaggtggtc gaagcggatg gactggctga
     5761 catcggcaac gactggtcct ggtactggac gggcactcaa tatgagtatt ttcacaagtt
     5821 ccggccggtc ctggactcag ggggatattt tcagaactgg tctcaaagca gctggaagcc
     5881 catgctgggt cccaacagcg atcccgcttt gaccagcttt gccggcaatc cttcgctgta
     5941 ttggctggcc gggattggca acccctcggc aatcatgagt ttcgagctga gaagcggatt
     6001 ctttgagggg tccgatacga tcgctcttga gaatggagcg gtttcagcac ctttgatcga
     6061 caccagcttt gaccatgctc aactgcctgt gtttgggccc actggtatcc gcctcttcag
     6121 cacaggctcc agcccctggt ctgaccttat gggagaattt ccctacaccg acggtacagg
     6181 taattatccc gtcacccgaa gcgagcagac cccaaacagc tatcacgaac ttgtcctcag
     6241 tatggatgac aggctcagga tcattgagtt tgggaacgac gcgctggata tgaccgatat
     6301 agatttaggt gctccaccca cactgcaagc ccttgcttac aacgacaagg tatataccgc
     6361 aacgtccgat gctgtgttcc agattggcaa caacgccata cagggcagag cagagattgc
     6421 ggacgcaaga aaactggcaa tagcgggaaa tgagcttctg gttttgacca aatccgggat
     6481 cgtctttctg gacctgagca acctcaatga aacccgccgc tataccctga gcggggaatt
     6541 tggggactac gagccactgg tattcttgaa tgacgaaacc aatcaaacca tcgcggtgtt
     6601 gacaaacaac cagggagata tctggagctg ccggaacaat gaagtagcca ggatattcag
     6661 caataattcc accgcgctgc caacgcagtt gggcatcaca tccatctcag gacaaagccc
     6721 catcatcttt ttcgggctgg gagaccaggc atatgccttg cggccggacg gaaccaattt
     6781 gagtggattt ccctatcata ctgatactta tgactatgag gcccacagac acgtctactc
     6841 agtcactctg agcggagata cctttatgca aatcccggtt gaagctgaag gttatcatgc
     6901 cgtctcgatc ctgccgtcat cagttccgca aaacgctctt agtctgcccg cgaggctgga
     6961 taacgaccgt ttctattatg acggagctaa cggcatcctc tactggtatt tcattgattc
     7021 tcaggcaaat ctctgctatc agtggatgag cgtagaggag aacccggtct tatgggcagg
     7081 acccagaaat gatgggacgg gtcagtttat gggggctgaa tcgcatagcg cgtctactca
     7141 aaccgcgctc agcgcttatg tgtatcccaa tccggtaaat agcagtccct tgcgggtcag
     7201 ggctgaacgt ctggactcca accccgtgga gctaaaagtt tacgatgtgg ccgggaaact
     7261 ggtcttagat aaatctttcg cggccaaccc actggacagt cgcgattttc aactggacca
     7321 caggctgtcc agcggagtgt attttttaac tgtcggaaac gggtcccgcc acaccaggat
     7381 caagtttgcc gtcatcaatt gagtcccggt catgaggtaa cagtatgaaa agattcattt
     7441 tgctaacaat cacattgatg atgatgatct ctctgtttgg tcaaagtgac atagatgatc
     7501 tgatttttgg acccgagaag caagatcaag cgcctgcaaa ggaccccaag ctcgctaagc
     7561 ccacagacga tgattttatt aaggtgaatt tcgccaagaa agacgcgagg ctggctatgc
     7621 tgttttccgc tctgttgcct ggagcgggac agtattatgc cgataaatca gctctaagca
     7681 cctggatctt tcccatagtg gaagtgggcc tcatcggcgg gattatctat tacaacgtgt
     7741 ccggcgataa aaggactgat gattttgaga agtacgccac tggtgagatc attacccaga
     7801 ctttcaacta cactgtggat ggggtcgatt acagctatac ctaccagggt ccccgctatc
     7861 aacggtctta ccaaaacctc actcaaaatg tactgatgag cttctttccc agtgatattt
     7921 acgatagcaa tttctttcgg ctggatgcca ccaataccca gcatttttat gaggacatcg
     7981 gcaaatacaa caagtatatc tttggctggg cagactggtt ctacaacttt gccaccgatc
     8041 ctatgacggg aaactttgta cttgataatc ccaactacta cgaaggtagc tttccaggtt
     8101 tgaatctgaa caccgtgtgg atttgggccg atactcagta cgattactca cgtcgctgga
     8161 ccgctaacgt ccagatcgat gattttctga atggccttag cggcaatcac atctctcccg
     8221 gaacgaccat ggcgtctccc atgcgacagg aatacatcca gatgcgcaaa gatgccaatt
     8281 cccagttttc tatcgcccgg attatgactc tggccctcgc cttcaatcat ctggccagcg
     8341 ctgtggatgc cgtaatggtc accaataagg tcaacaagac ttacatcacg caaaacaatc
     8401 tccaactcaa gtattacacc gctttccgtg acggcagggt ctccccctcc ctcggttttt
     8461 cctggtcttt ttgatatgcg tatcgtcctg cttgtgctgt tgaccacact gctggccatg
     8521 ccgctaatcg ctttgccgcg aatagcatac attggtatgt cggcagccct gcctgggagc
     8581 gcggagctgg ccttgggaaa acctacccgg ggcggaataa tgatcgcggc tgatctgctg
     8641 tcatggctgg cttggagcgg ttacaaagat gaagcacttg atcttgagga tagcttcaag
     8701 cgctacgccc aggcttacgc cggtgtaccc agcaacagca atgatcgata ctatcagcat
     8761 atccaacagt attatagcag cgatgagttc aattcgtttc aggagatgat ggccagaaac
     8821 ttctatctga tatacaatta tgacccagaa gggttttctg aataccttgc ggccaacctc
     8881 tatgccgacg aggaagggtg gcaatggcag agtcctgagc atcatcagaa gtttcgcgac
     8941 ttgcgcaaac agcggcagaa agcaaaaatg tatcagaatc tggctttggg ggccttgctg
     9001 ttaaaccggg gaatcagtat agtggatgcc ctgtttttga gtggtaagaa atccgaggca
     9061 gagcttccgg tttatttcag tgtgtcaccg gagcgggctt tgatcttgaa ctatcgtttt
     9121 gaattttgag gtttacagat gcgtagatta ttgataatag tgcttttact gggctcggtg
     9181 ggtctgacgg ctcagagccg ggggttgaag tttttgaagt ctttggtggt gcccgggcta
     9241 tctcaggtgt cagaaggccg caattacggc tacgccatga tggtgagcga aatcagcatc
     9301 attgggggca tgatgtatct gaacagcgag gagaagctca aagccagaga atcatatgag
     9361 tacgcgttga agtatgctca catccgccca ggcgggtatg aagaaagcta tttcagagac
     9421 ctcagccgct acaacagcgg tggattcgac gcggatggat tcaacgccag agtgaggaga
     9481 gacgccatga atctctatcc tcacgatccc gtagcccaac agcaatacat cgattctcac
     9541 gcttatcccg aagacatgta ctggaattgg gattcagctc aaaagcgggc cagctacagc
     9601 aaaatcagga taaaaaccca ggacctgcgt gattttggac agatcgcggt aggagtgctg
     9661 ctggtcaacc acttaatcag cggtatcgat gtgctgagat acgactcaga agggcgccgt
     9721 tcccaagtct atatgagcat gaagggcaaa cagcccatcc tcaatctcag cgtcagcttc
     9781 taggctgtca tccccttttt tctcacttac aggtatcgca cgcttatcct gtgctgaggc
     9841 atgggatagg cgtgcgagga gcacgggata ggcaaaggag gctaaagggc aagattgctg
     9901 tcagcccgtc cggatcagaa aatggcttta atcagcgttt ccgggtccag tctaaaggga
     9961 gagcccttac cttcaaagaa gctgtgagca tcgatcagat ggatgctgag ggaggtccac
    10021 cgtacactca ggcccatact tttattgaat agggtggtga tcgccttgcg gtgtacgccg
    10081 ggatgtccaa aggggcatgg aagcttgcct ctgactacat cggtgctcac cagatacttg
    10141 tcttccacca gcacttcctc accagggaaa tcccagctct gtttagtcag atannnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacggcgt tgatcatacc ggggctgccg
    10321 cagaggtagg cttcggtttt gaggggatcg cggataacgt ctttaaagta aggcatcaca
    10381 aaaccagtct tgccctgcca gttgtcgtct ggctccatat gagataaaac aggctcataa
    10441 ctgaaagcgg gaaacacctg ctcgaaagcc ttcatttcgt ctgtcagata gaggtcttta
    10501 gtagtcctgg cgccaaagaa ataggtcatc tttctgtcgc aaccggttac cttcagatgt
    10561 tcgagcatcg atttgatggg ggctttgcca ctaccccctg ccacgaaaat gatatcggcg
    10621 tcggtgtcac ggataaagaa atccccgtag ggtcccgtaa cctctacctc gtcgcctacc
    10681 tggaggtgtt ggtgcaccca ggtggtgcag atgccttcag ggacgaggcg gatgatcagc
    10741 tgaaaacgat cggaatcttc agggcgggac gagatggaat aggccctgct gacgctttga
    10801 cgcatcttgc catagggctt ggtgttgacc tgaacatatt gcccggccag aaaatcgatg
    10861 gtttcgcctt cctggagttt gaatgtgacg cctttgatgt cgtaggtgta atccactatc
    10921 tccgaaatgc ttgtcttata tttgcggatg ttgaagatag catcagggat ttggatagcg
    10981 atgtcagact tcactttcac ctggcaagcc agcctcacgt ttgtggccat ctcacttttg
    11041 gagagcaagg gtcgttcggt gggcaaaagg ggtcctccac cttcccgcac ctggcacttg
    11101 caagcaccgc aactgcctct gccaccacag gcggaaggga taaatatctg cttgtcggcc
    11161 agagagttga gcaggctact gccacctttc acggtcacat cccgtttacc gttgatctgg
    11221 attttacact cgccatagtt gctcagccac ttctgcgcga tcacgagcag gatccctaat
    11281 acagcggaga tcgcggtgag aatgccaatg tctttcagaa tagtcaataa cacttccgct
    11341 ctcccctatt gaatttgcac aataccggaa aaaccgataa aagccagggc catgatccct
    11401 gtgatgatca gactgatacc cgcgccctgc aatccagccg gcacaagttt ttcttttagt
    11461 ttggcccgga tgccggccag catcagaatg gccagcagcc acccgatccc gcttccggcc
    11521 ccaaaggcaa tactttgcaa gaaattatag tctctgatca ccagaaacag gctgactcca
    11581 aagatagcgc agttgacagt gatcagcggc aagaagattc ccagggagta gtacagggcc
    11641 ggcgcgtagc gttccaccac caattccaga agctgaacaa aagccgcgat cacaataata
    11701 aagacgatga attgcagata catgatgccg aacgggacca acacatagtg gtacaccagc
    11761 cagttcaaac cagcggtggc ggtaagaacg aagatcaccg caattccaag tccggcagaa
    11821 gattccacct ctctcgaaac ggagaggaag gagcacatgc ccagaaagtt ggtcagcagg
    11881 atattgctgg taaagatagc ggcccaaaac agcacaaaag ctgaaatatc cattatttgg
    11941 cccctttata atagtaggtg ttgatagccc agatcatcaa agccaggata aagaacgcgc
    12001 tgggcgccat caccatgatc gaccaattgg tccagccggc ccccatcact ctgaaaccaa
    12061 acagggtgcc aaatccaaac agctctctga caaaggcaat caacaaaagc accagggtgt
    12121 atcccacgcc agccccgatc ccgtctatca ttgcgtccac aggcttgttt tgagaggcaa
    12181 aggcttccgc tctgcccatc aggatgcagt tggtgatgat caaaccaacg tagggtccca
    12241 gctgtttgct gatttcgggc atccaggctt tgagcaggat atccacaatg atcacgtaag
    12301 cacctatgat cagagtctgg gccatcattc tgacgctgcg gggagtatat gatctgatca
    12361 atgagatagt ataattggcc agggccaggg agaagatcac ccccagtccc atgatcaggc
    12421 tgttgagcat caggtttgta accgccagcg aggaacagat tcccagaatt tgtttccaga
    12481 cgctgttttc tttgaaagcg ctgacgatga agatatcttt tttggtcatc gctggcctcc
    12541 cgggtgattt tgatcgtgga gcttttgaag ctccgtgctg agcatattca ataccgcggc
    12601 gctggtaatg gtagccccgg ttacttgatt aacctgattg ggattggctg cttgagcttc
    12661 aggaatcagc tcgagatcca taaacttgcc gtcggtgatc acagtgcgat aacggaactg
    12721 ggcctgaaac cactcttcgg aaattctggc ccccagtccg ggagtctcca tctgttcata
    12781 tatggccaga tctctgatct gagttaaatc cggggatagc gacaccagtc cccgcatcga
    12841 accccagagc cctttgccgg gaatatccac gcagcgcacg atcacggaat cgtttatgct
    12901 cacagcccag gaatcccgtt cctcaccttt gagagggctc tttgtgatgt attgatcata
    12961 gctttgggga tatcgggaag tgatgtcggc cgcactgatc ccggttgcct ttgagataga
    13021 tgaggcgcag agttgcagaa ccagcttttg ataactttcc cgtttgtagg tctcgatccg
    13081 gttttcgcta aaacgaaaaa ggacggccag gattcccacg aaaatgaggc tgaccattat
    13141 cataaagatc acggggtagg acatggtctc aatgaaggga actctttctt tgctcatgcc
    13201 ttagctccct tttttttagt ccgtttgtcc aaatagtact cgatgatcgg cataaaagaa
    13261 ttcgccagca gcaaggcaaa cataaaacct tccgcgaaaa gcgagtagcg acggataaac
    13321 acagtcagaa acccgatcag aacgccgtaa atccagatgg ccaattttcc tttggcctga
    13381 gacacgggat cggtggtcat gtacactact ccaaacagag ctcccccact ggccagaaag
    13441 taaacaggat tctcaccggg ataaaagatg aatccaaaga ccagcagaga cagcaccgtg
    13501 gctaccatgg gctgccattt ggctgtcttg gtgaggacca gatagatccc tgcaaggatg
    13561 atcagcaggg cggatgtctc accaatcgag ccaggcacaa aacccagaaa ggcgtcttta
    13621 aaggaataga tcagctcttc cttgagacga taatgtccca tgatcgtggc actggtttcc
    13681 attccggctc gggctgtcca actcgccaaa ccaccaggga aatcgcttag taagaagggt
    13741 tttagccagg cgatcgtcat ctgctgagga aaggcgacat acacaaaggt ccttcccaga
    13801 atggctggat tgaagggatt agtgccaaaa ccgccgaaca ccattttccc aaacgcaatc
    13861 gataccacgg ccgctaccgc actcatccac aagggaatag tgggaggaag cgtcatcgct
    13921 accaaagtgg ccgtgacaaa ggcagccata gtgaccttgc cgcctttttt ggtgcggata
    13981 aagaggtatt ccgtgatata ggcagctata ttggccacca atacaaccgc cagcaccctc
    14041 cagccaaaca aataaaccgc gaagatcaac aagggtgcca gcgcgtagag cacgcgattc
    14101 atcaccgcct gtttcatcaa tctttcaaac atgatgggtg tctcccttat cattggaagc
    14161 atcactcaga gcaatcaaat cctgagtggc atgagatgta tgtgattgcg agcgctgcca
    14221 ggcagccaga aaggtccgca gttccggatc gcgggaacga ttgcagatca ggcgcagatg
    14281 gtgttcatct ttaccatggc tatgaatcgc gactccatcc agagattcga tcaaaaatcc
    14341 tgccaggcgc tgctcttgtt tttcaatacg cagttgcaaa atacaggtgt tagtatcttc
    14401 cggactggaa gcaaacgatt cactgagtgc agggatggcc tcccgtagcg atgctgaacg
    14461 agtgtctgcc agatcgcaat ccggcaaagc ttccattcta tgtctcattc cttttaccgc
    14521 cgatgaagct tcaataagta ccagggtcaa tttccggagt gctcagggtg tcggcaacag
    14581 gttccgggcc ccggctgagg aagagcctca ccatggcgtt tttgtctact ctggacccgt
    14641 tgggaggaga agtcctgatc acagtgttag ctggataact ctggctgtac aacgaatccg
    14701 ctacagatgc tctcaagcca ctgttgcgca gcgtgatgaa tgcctcttca tagcttcttc
    14761 ccactactcc tggaaccgcg acgatctgtg atcccttgca gatcaccaga tacaccgtgc
    14821 cctctagctt tgccaaagtt ccgggctccg gatgctgctg aatcactgat tcgattttgg
    14881 cggagtccga ccatacggaa tctctcacca cggcatgcag tcccgcttcc ttgagcaggc
    14941 gtttggccgt gctgatgttt ttgccgatca agtctggcac ttctatgttg ctgggtttgg
    15001 cgaagatgag cggcatgatc acctgccctg tcaggaaggc ggttacgatg atgacccccg
    15061 ctccgatgag agcgaggtag ccggccttgc gtagtttatt gcggtccata gttttacctg
    15121 attctttgta gtttggcccc gaaagctcca tccatatgat gaataaacgg gatactgcga
    15181 aacatcccat cccgcacgca gttgtctgga atcaaagccc ccggagggga taacacgaaa
    15241 cgggtgtttc tggccagaaa ccgttgaatc tgatgctcgt tctcatctgg attcatggtg
    15301 caagtggaat aaatcataaa tccctcgggc cgcacaaagt tggcagcgtg atcgagggct
    15361 ctttcctgaa ttttgatcag ttcggagata ttggaatggg cctgccaacg cagatcggct
    15421 ttgcggccaa aaacacccca gccggaacag ggggcgtcta ccaatacttt gtcataagca
    15481 ggtgccacgg gtccatattt gaaagcgtca gcggtcacga gcctgatatt gctgagctgg
    15541 agacgatcgg ccgcctgctt gagcagtttc atcttgttgg ggattttgtc caccgcgatc
    15601 acttcaccgc tgttttccat cttttcagcg atataggtgc atttgcctcc aggcgcggca
    15661 aagaggtcca ggatgttgtc gtctttcctg ggatcgagca ggtccactat catagccgcg
    15721 gagctatcct gaacgctgaa atacccttcc gagaaggcca catcctccag tacctggtca
    15781 gcctgatcgg tgctgagcat cattgctcca taaccggttg gcttcagttc cacacctcgt
    15841 ttggcgtagt actgcatcag tttttctggg gtggtggcgg tggaattgac cctgatgtgc
    15901 aggatggggt tttcattgaa atagagcatg aggtattcag cgttttccag cccccagagc
    15961 tcaacccagc gtttcaccag atcgggtgga tagctgtgtt cgaatgccgc tttgctgata
    16021 ggatcatcgg gataactgac actctggtcc ctctgccagg ccctcaggat ggcatttaca
    16081 aaatctgcca cgttggggtt gaacagctgc ttggcaagct ccaccgtttc gtttacagcc
    16141 gcgtgggaag ggatggaatc cagatagata agctgataga gccctacata gagcagaatt
    16201 ttgatcttca gatcggtgga agcgtatttt atagcgtcgg tatgctgact gattataaag
    16261 tccagatgcc cccgcatctt gataacgcct ttgaccaacg agtagaacaa tgaaagattt
    16321 tccccggcct gcttgattct tttagacctt tgatgcaaga gggtgtctga aaactccccc
    16381 tccttgaata ccttcagaat ggtctgataa gcttcagccc tgagcatgtc aacgagacct
    16441 ttgataaagg cgcttgatgg tttccttgat cagctgctct gagcccatcg attttgcctc
    16501 ttcaggcatc aaagcaagct cacggcggat gtctttggga ttgaatccca gggtcacaag
    16561 cgcggattcc acctcatcca ccacgcttcc ctgcaagata tggtcttcgg ccttgaaatg
    16621 atctccaagg gcggcaaaac cacccctcag ttccactatc agacgctgcg cgcttttctt
    16681 gccgagtccg ggtacccggg ttatcaaacc ctcttccccg ttttcaatgg cgcgcacaaa
    16741 agtggagatc ggcatcgtgg acaggatgga cagggcaatt tttggcccga tcccagagac
    16801 cgatgtcagg attaaaaaaa gctcgcgttc ggccagagca tgaaacccaa agaggcggat
    16861 atcatcctga gcgatatgca gatgcgtgaa aagctgacag gaactacctg ccgcgggcag
    16921 tttctcaaaa gtactgatcg ggaccttgag atcgaaggcc aatcccgaac tggtttcaat
    16981 cgtcacgctc accgggtttt tggtgatgag ctttcccttg atatatgcga tcatgacaga
    17041 aatcttatcc ggttaaaatg gcacatggcg atcgccaaag cgtcgtaggc gtcatccggt
    17101 ttgtcgctgc ctttcagttt aaaaagctgt tccaccatgt atctcacctg ctgtttggat
    17161 gcgccgccgt ttcccacgac cgcttttttg acctctctgg ggctatattc aaataccgga
    17221 agtccatatc tggcaaatgc cagcaggatc actcccctgg cctggctaag agtcagcata
    17281 cttttcacat tcttatgata aaacatactt tccaccacag cgatttctgg cttgaattca
    17341 gccaggaccg cttccactct ggcaaaaaga gcggcgagac gttcaggcag agtggtatag
    17401 gagctgatgt ccacgacatc gcaacccgcg gcagttatca ccctgccctg agtctccaat
    17461 agtccatatc cgcagacgcg gctgcctgga tcgatgccaa tcaggaccac tattccctgt
    17521 tcaggctttc catgacttcg tcggacagct caaaattggc ataaaccttc tggacgtcat
    17581 ccaggtcctc aagcatttct atcagtctca aaagcttgcc agccacgtca tcggcattga
    17641 tggtgttttt ggggatcttg gtaagctcgg cgttgaggac agggaagccc aggctttcga
    17701 agtttgccag caccgtatga aaatcactgg gggaagtaaa gacgtcaaag ttctcgtcgt
    17761 tgagctcaat atcctccgca ccggcttcca gggcctgcat cataaattca tcttcatcca
    17821 ggcccaccgc aggaacgtta aaaaagcctt tctgttcaaa attccaggat acggctccag
    17881 tctcgccaag gttgccgcca tacttggaaa aggtgtgacg gagctctgac acggtgcggt
    17941 ttttgttgtc ggtcatcaca tcaaccacga tacccacgcc gttgtggccg tagccttcat
    18001 aagtgatctc ttcataagcc gcgccttcaa tctcaccggt gccgcgtttg atggcgcgtt
    18061 caatattgtc gcgtggcatg ttggcagctt tggcggcgtt gatggcggtt ctcagacggg
    18121 ggttcatgtc ggcatctccc ccaccgctct tggcggccag aatgatctct ttgacgatgc
    18181 gggtgtagat ttttccgcgt ttggcatcag tagccgcttt cttgtgctta atggtgctcc
    18241 atttattgtg tccggacata aaaacctctt gtcattcttt atgtattaca gtctgttatc
    18301 ttctggagtc agttgtgcat gactgccttt tcatgtcaag taagaaaagg ggaagccgaa
    18361 gattatcggc ctcccctgga tcgcagctat caaacgtctg aatatcagac ggcttatgct
    18421 tcggaatcct gccaggcggc caccaccttt tgagtgatgt cctcaggcac tttttcgtaa
    18481 tgagagaatt tttgggtgaa gcgacctctg ccctgagtga atgatttgag ggcgggaaag
    18541 tacagataca tttccgcgac cggcatttga gcgttcagat actgcttctt gccgcgttgc
    18601 tccataccca tgatgcgtcc gcgacgggtg ctgatgtcac ccatcacgtc acccatatac
    18661 tcgctgggaa cgatgatcac caagtcgtga atcggttcca gcagaatggg tttgcatttc
    18721 ttgaagcctt ccttgagggc catggaactg gcaatcttga aggccatttc agagctgtca
    18781 acgtcgtgat agctgccata atagacttcc acgctgatat cgacaacctg gtaaccggca
    18841 ataatgccct tctccatcgt ttccacgatt cccttttcga tcgcgggaat gaaattggag
    18901 gggatcacgc cacccacgat ggcgttgata aaatggaatc cttccccgcg ttccactggt
    18961 ttgatgcgga aatagacttc cccatactga ccacggcctc ccgactgttt tttatgacgg
    19021 tactgggatt cggcacttcc gatgatggtc tctttataag gtatgcgggg ctctttcatc
    19081 atcgcgtcca ctttatagcg gtttttcaac ttcttgagca caagtgtcat ttgtgcttcg
    19141 cccatcccgg aaatcacgtt ttcatgcgtc tcctgattga gctcaaagcg gatggaagga
    19201 tcttcagcga tcacacgctg cagagaggag ccgatcttgt cttcatcaga ctgattggcg
    19261 gcacggatca tctgccaata agtagctgtc ggcaattcag gcatcggtag cgccagcctg
    19321 gagttgagag ctgtgatgct gttcagcgcg cgggcgtttt tcagcttgac cagagccccg
    19381 atttctccgg ctctgatctc ctgagtgtcg tggcgggttt tgccaagcat gtaatacatg
    19441 tttccggctt tgtctttggc atctttctcg gggatataga attccgtgcc ggccttgaga
    19501 ttcccggaga aaatcctcac ataggcatat tcacccatgt tgggatcgct gtagagcttg
    19561 aacacgtagc ctagcagctc gccgtctggc gacaccataa agctcttgcg ctcttcgccg
    19621 ctgattactt ccagagcggt tgattccacc ggtgatggca gataggcgag cgtggcatcc
    19681 aaaaaggcat ttacgcccac tccggtgttg gcagagcatg cgaaagcggg aatgatctcg
    19741 ccgctggcga tggctttttt gagccctggc atcatctgct cctggctcag ttccatgttt
    19801 tccaggaagc tgttcaacag ctcttcatcc gtctcagcca cggcttccat cagatgcagc
    19861 ctggcttctt ccgcgaggtc atgcagttca gccgggatat cagagacacc ggcaggacgg
    19921 atagctttca tggaaatgac gtcgatcacg ccttcaaaag agccttcttt gccaatcgga
    19981 agcatcaggg gggtggcttt gaggcctgag ttttcataga tggagtcgag ggtcttctgg
    20041 aaatcggcgt gttcgttgtc catgcggttt acgaccacga ttttaccaac cttcttatgc
    20101 tcgagctgtt cgatcgccaa ctccaggccg acttcgaagc ccgccgcggc attggcaacc
    20161 aacaccacgt tttccaccgc tgggatggcc acaatggcgt ccgctacgaa atcgggagtt
    20221 ccgggagcgt ctaaaatgtt gatcttgtga tcattatagt ttacaaaacc gatactcagg
    20281 ctgagcgaca tctttttcgc ggtttcttcg gcgtcaaaat ccatcaccgt attgccttca
    20341 tcgatcttgc cgatccggtt ggtggcatgg gtcaggaaaa agatctgttc ggccaggctt
    20401 gtcttgccag ctccacctga tcccataagg agaagattgc gaatcttctt aaggtcgtat
    20461 tctttcattt cttcctcttg ttattatctg taacgcactg taatatatga tagaaagtgg
    20521 tgttccacaa agtgtttacg tagaagaggc catgtttttt caggggaggt gcttgtcaat
    20581 gcttttctca ggcctcgcgg agttggcact ggtcgcagga gttcaggcat tgtttgatcc
    20641 cgctgaatag ttgcccttca tctggctcgt gcaacaactc ctccagctta tctttgtttc
    20701 tgaagcccag ggccagactt tggaggattt gaatctgagc tttgggattg tcgataggtg
    20761 ttatcagcag cagggccagc tggacaggat ccccgtccgg actgtcccac tcgatccctc
    20821 tgggggccat caaagcataa acgtaagatt gctctacgct gtcgatcctg gcatggggta
    20881 cggcaatacc ttttcccaga gcggtgctca tctgtgcttc ccgcaactcc acttcccggg
    20941 ccagggcggt ttttggaatt ccgaggtcct gagcggcaag gctgatcaaa gcttcgatcg
    21001 cctgagttct atcggccgtg agcttgttta tcagtacatg ccggggcttg aaaatcacgc
    21061 tgaagagctg atttctgatc ttgttcaccg cgtaagacag ccaggggccg aaaatgatgg
    21121 tggataagac cgcggatgct ataatcgcca ccagcacttc cgtattgatc agtttcagcg
    21181 aataagccag catggccacc acaatgtgca tttggcctcc gggagtatga gcaatggcaa
    21241 tggtcaagat atcgctctta tcctgacggc tccatttggc cccgagccag gcccccaaaa
    21301 accttccagc gaccccgacc accaagaaca agagcgttaa gaagaggtcg aaattggcca
    21361 tgaaatccag atgcaacccg atgctggcaa agaagatcgg cacaaagatc gaatacacca
    21421 gccggttgac cacaaagcgg tccttttctg tgatgtgggt tgcttcgccg agtacgaccc
    21481 cggcgatgaa gaatccaaac agagcatgaa taccgattcc gagagtaagc gctccaaaca
    21541 ggatgcccag caggatgatg aaagtaattt tcaggcctga tgactggtcc acctttttat
    21601 ggaggaagct caccgattta tccaccacag atttcacgat cgtgagactg atcaccgtaa
    21661 agcccagcgt cagaagcatc aactgaccga tatagcccaa ttccaaagag ccgcgcgcga
    21721 acaagccaag gatgatcgtg aataccaccc agcccacgat atcgttgatg gtgagggctg
    21781 agatgatcaa aaaacccaca tccgtcttga ggatgttcat gtctcgcaat cctctgatcg
    21841 ccacaggtaa agcgctgatt gtcatgatca cactcatgaa aagagcaaac agcagcttat
    21901 gttcaggatc caccaaatag cgatctggta acagcaacat cggcacaaag ctcaccagca
    21961 ccgggatgat cagatcactg aaactgatga tcagggcctg agtcttttgc ctccagatac
    22021 gcgagaaatt cacctccagt ccggtttcca tcagcaagaa gaagttccca atccaggcaa
    22081 ttgtttccag catactgcgc tggacctgat tatcgggaaa caggcccgcg tggatgccgg
    22141 gagccagttt tcccaggata gtgggcccca ggataatgcc caccaaaagc tccgcggtaa
    22201 cgctggactg ctttagcttt tcaaagaaat atccggcgat tttgcatatg cccagcaaca
    22261 atccaaactg gatcaggaag agggtcaggt tatgttctgt aatcggtccc atatattcat
    22321 cccttgattc tttgcactta cagatatgat aattgaaata gccgggatgc tcaaggattt
    22381 ttcccttcag tgctgtccga aaatcccatc agcagaaacc caggatcaga gccaggactt
    22441 agcaattacc aggataacta tctatcgcgg aacgtaatag cgttgtcgat aaccccttcc
    22501 caaccagaga gtctaaaaca tcacggtagc aagccggacc caactcgcaa ttatgggcct
    22561 tgggtcgggt atggtagctg tggcacaggg ccctgcagat aaggggtgat tgggctgcaa
    22621 aaagaaaggc tcccagtgtg ggagccttta gataggatta tacgttaatg cttagtcgaa
    22681 cgcgagtttg atagtggtgc cgagggcata gttgtcttga tcggaaacgt cttcatccat
    22741 gatgctgtag gccgcgaagg ctgcaagatg aagtaaattg ggaaccagct ctacttccag
    22801 tccaccgttc agctcaaggc ctgtatcgag gaccatacca cccgctccaa agaccttaac
    22861 gcctttcatg gcttcaaact tggcttgaag ggccgctcca agatacatat ccggatttcc
    22921 ggtagctccc caccagaggc caaggccgtc atggtgttcg ccgatcccaa gcaggtatag
    22981 gccattctga tagtagccgg agagggtcat gaggccgttt tcactaatgt agagaatatc
    23041 ggcgtttact tccaggggtt gcacgtcgac accagctttg agggcaacgc caaatgataa
    23101 atcgctctct ttttcaagat caccacccac aaaactgaag ctgtcgtttg attgcacaaa
    23161 caacactccg tcaactgtga taggatcgat attcatggat gcgtaaggca tcacagagat
    23221 gtagttcagc tgaatcgcat cttccgtagc aattggacgt acccggctcc aggtgagata
    23281 accttgcaag cccgcttcga ctggtttggc ggtgtcgagg gagaagataa tgccctgcat
    23341 gtcatcataa cggttgtaat agttgccttc ggcatacttg ccgaagccca gcattccatt
    23401 gattccatcc ggggtgctgg tatggaaggt gactccgctg aatgtgtcat ccaggatcag
    23461 gctgcgatga tcggcccagt attgctgacc gactctcacg ttggctttga tcgcttcaat
    23521 ccggtagtcg atgtaagcct cgttggtctt gacattgatg ccgttggtag ccaagccgcc
    23581 accgtctcca ccccatacga ggttggcatc accgccattc tggccgattt caaacttggc
    23641 ccttaaggtg agaccaggca caagctctga tttgaggccc agctgcagac ggttgtctat
    23701 gtgagcgcct ggtgtgccca tatcgtcatt ataagcggca gcgcgggtgc ggaacatgcc
    23761 gtcccactta aaatcgtaag cgaagagact ggcggccatc aagaccggga tcaggaccag
    23821 aatcatcctt ttcgttgtca ttttgtttct cctaggtatg gtgttttacc gagaatttag
    23881 tcatgatggg gcctcaagca agttcaatcc acaaacttga gcatttcgaa gcccaattcg
    23941 cgctgcatcg tgatgatgtc ctgctgcagt ttgggaatca caggtacccc ttccgcaagc
    24001 acttgcagcc gcttttcatg ttctttctca cctgctaccc agattctttg ttggccagga
    24061 tatttctgag aattctgtag ttggcgacag atttcagagc tggtggattt aaactcatcc
    24121 agttccgtaa agaaggcgat gttgatcgcc ataaagaaat ggcccaagcg ataaggggct
    24181 ggtttgccgt ccgcgtcctg gcccagcagg ccgttcagga agcttccatt ggccagggca
    24241 gcgcaaagaa tttctaccat ggtcgatagt ccatatccct tatgagagcc agaaacttcg
    24301 tccgtgcctc caataggcag cagagaagcg gtttgtttga ccagatcaac caggagttct
    24361 ggagtgcggg tatgagcgtt gccatccaaa tcgatagccc agccactggg tgtgtctttg
    24421 ccctctctgg catattgctc gatttttcca cgctgagaga tggaagtagc ggcgtcataa
    24481 ataaaaggaa agcccagctg agtgggcgcg ccaaagcaga tgggattggt gcccagaagt
    24541 ggctgaactc catgcgtggg gcaaactgaa ggacgggcat tggtaaaagt gaggccgatc
    24601 atatcttgcc gggtggccat ttcggtgtag aatccacaaa taccgtagtg ggtggaattc
    24661 ctcactgcca cggctcccag gccaaattgg gcggctttgt cgatggcagt ctgcatggca
    24721 ctttttgaga tcacatggcc catgccatga ttgccatccc acacagccgt ggccatcctg
    24781 tccttgacga cgtcaatcct ggtgatgggg tcctgaatcc cagcccgaat ccggtcgtaa
    24841 tacatcttca atctgccgat gccgtgagat tcgattcctc tcagatcgct ggcgatcagg
    24901 atctcggaac aaatctgtgc gtctgacgcc ggcaccccta ttctggtgag tacttcggtc
    24961 atgaattcct gcagggtgga aactggaagt ttggtcattt gatctccgtg gtttgggatt
    25021 gtggatcaac cgcagaaagc gtgcgctctg agcggaggaa agccattgaa gttgatcgac
    25081 gaatagctct gagtataagc ccccgtcgtg aaaatatata ctctgtcacc cggctgggcg
    25141 gtctcgggca tgtggtattt atagtgttca tagaggatgt ccatgctgtc acaggtcgga
    25201 cctgccagaa tgatctcgtc ggcctggcct tctctgtcga aaaagatggg aaacttgatg
    25261 gattcgtcta tggtctcgat caggccgcca aacttgccca catccaaaaa cacccattga
    25321 tagatgttgt ttttggactt tctggagatg ttgacaatct cggaaaccaa cagccccgcg
    25381 tccgccacca aagagcgtcc cggttccagt atgatctgcg ggagatgatc gccaaaatcc
    25441 tctttaataa acctgatgat ctcgttggtg taatctgaga cgtcgaaggt cgggtccaca
    25501 tagttggcgg gaaaaccacc gccgatgttg atcatcttga gttcgatgcc ctcttcggcg
    25561 accgcgtcaa agagatattt acagcgcgcg atggcatcgt cccactggcc aatatcacgt
    25621 tgctgagagc ccacgtgaaa ggacacgccc cacggctgta ctcccaaatc ctgagcttgg
    25681 atgatcagat aatataacat gtctgaatga gcgccaaact ttctggatag tggccagtct
    25741 gcaccggtac cttcagtcag aattctgaag aagaccttgg ccccgggagc ttcctgcgct
    25801 atgtttagca ggtctggctc gctgtccgtg gcaaagagcc ttaccccttt ttcatagaaa
    25861 tatgcaatgt cccgacgctt tttgatcgtg tttccgtaac tcaagcgctc tggagatatt
    25921 cccaggccca gcataaggtc cagttcataa cgggaggcca catcgaaatg agagcccatg
    25981 gagtccagca gtttcagcac ctctggcatc ggattggcct tcacggcgta ataaatggcc
    26041 gcaccgggca gacaggtctg cagctcccga tatttcttgc ttaccaggtc caaatctatc
    26101 accagacatg gcgtctgtaa ttgggtggca tagtcacgca gtttattgaa acgttcaggg
    26161 ttcatgtagc gctctacatt gaagtgatat gtttctttat acattgttat tatccctcaa
    26221 ttgtcattat tgccggccac gaaaatcaac gccgtaattg tgtcaatcgt taaaggactt
    26281 aaagacaaaa aaaaggcagg agagcatcct gcctttggaa acgaatttcc aatcgcggtt
    26341 atttcttgac agcttctttc agcttctttc ccgctttaaa gatcggaact ttacgggctg
    26401 ggattttgag ggctttgcgg gttttgggat tgatgccgtt acgggctttg cgatgggcta
    26461 cgctaaatga accaaaacct accagagaca ctctgtctcc cttcttaagc gctaacgtga
    26521 taccttcaag aacggccgca agagccagac cggctacttt ctgtgaaata ccggcatctc
    26581 ccgcgatttt tttgatcaaa tcatctctgc tcatcttacc tccttttagt gttgtgatac
    26641 gagtaagaga tattactgga ttgatcaaaa ccggccattg aaaatctgtc aacaaaaaaa
    26701 accagtagtg atgcgggttt gagggctatt ttccactccc atgacctctc caatatcgct
    26761 aactgcttaa atatcaacat aaaaccccca agtgaaaatt atccacgaac tccggatatt
    26821 ttttcatctt acgcagtgac aagctttttc cattgacaaa ttatggagtt cagagagctt
    26881 tgaatcattc aaacaattac tggaggtttt aatgaaaaag cacctgtttt tatacttggc
    26941 cgcagctacg ataatcctga ttctgatcag ttcctgcgcc aagaccaaaa ctgaagtgga
    27001 gacggtggct gctcccccca aaccgatcga tcttgcccac gctgccacca aagccggtga
    27061 agaggcattt cagaaccagc tcctcgatga ggccaagctc aagttcaaag aggccatcgt
    27121 gcagtttgaa gcggccgctc cgactgccgc ggctaccgat tctatcgacg ttaatatcga
    27181 aagaatgcat atcaacatcg ccaaagtgaa tatggacctc gccactgaga gcactgccgc
    27241 gcaaatgcat tcggaagcaa ttgaacaact tcaggaagca ctggacagct tcaaaaagat
    27301 ccagcctatc acgatccccg ccaccgaaaa agacgagatc atcaagtctc tctacatcag
    27361 gctggcctat gccaatcagt ccgccggaag ttatgaagat gccctgctgg cctatgatga
    27421 agtcttgaaa atggagcccg gcaacgaaga gatcctcaac aacaaatacg tcattctcaa
    27481 cgacaatatc aaagatgaaa ccagagcttt ccaggttttg aaggattacg cccaggcttc
    27541 caacgattac cgtgcttatc tgattttggg cacccgctac aacgaaaaga aaaacgtcgc
    27601 ggaagccacc aaatactacg aacaggccct cactctcaac aagagcgctg acgtgctgac
    27661 ccgcgtctcc gatttctatc gctccaacag caattgggcc gcttccaaca aagtcctcga
    27721 tcaacttgtg gccacgaagc cggacaatgc caccctggca gccgtttacc gtctgatggg
    27781 agacaactac agcaagctga aaaacaacgc caaaaaagcc gacgcctggg aaaagtcttt
    27841 agccctggaa ccaaacggtg aaatcgctct ggctgtggcc aaacactatt acgacgccaa
    27901 aaactacaat aaggccgtca cctatgccac ccaggccatc aatcaggatt ccggaaacgc
    27961 cgcggcgtat ctgctgagag gagactgcta ttacaagcta aagaaaaacg cacaggccaa
    28021 agcagacctg cagaggatcc agaatgatcc gcagcatggt tctactgccc agaagctgct
    28081 caaagccatc aaataataca tcttatgcca ttacgaaaac cccagcattc gtctggggtt
    28141 tttcgtttcc cgcagatcac gccgattctt acgcagatca cgctgattta gatttttggt
    28201 tgatggtttg caaagcttgg aaaagactat tgacttacca tctaaactct ctaacccctc
    28261 tcaaccttgt caactaagca atctgcgata tctgcgtttt catcagcgca atctgcgtga
    28321 accaatcccg acaaattgga tttgacaaaa gcctcgcgtt ttaaagacta gaccgctgat
    28381 ccatacaaaa gagataaatc tggagcaatc gatgaacaaa ctaaagacgg acggcacgat
    28441 caaagccatc tacgggttta ggaaagacct caagctcagt cgccctctgg tgggaccaag
    28501 ggtgccagct ggctttccat cccccgcgca ggattacatg gagggcagcc ttgacctcaa
    28561 tgaatatctg gtctcgcatc cggcttccac tttctttgtg agagtggagg ggttttccat
    28621 gatcaaagcc ggcatctttc cagacgacat tttggtagtg gaccgctctc tggaacccgc
    28681 gtccggaagg attatcgtgg caatttacga tggtgaactg acagtcaagc gtttgaagat
    28741 cgagtgcaac cactactggc tggaaccgga aaacgatgaa tttgagccca tctgtgtgga
    28801 agaagagaca gacttcctgg tctggggtgt ggtctgctgg gtaatccata aagtttagat
    28861 gcgttcttta aggtttgttc tgctaatcct gcttctgaac ctgattcagg gctatgtgta
    28921 tggggaaaca agcctcctcc aggaaagatt gatcgctctg gtccccggga ttggaaccta
    28981 tgatctgagc agcggcccca tcatcgtcgg ctcggagcag atcagcactg gagacgttat
    29041 cctgctgcgg ggaatcgact atcggatcga ctatcggaaa gggcagctta ccatcttaaa
    29101 agacctgacg gcggtctata tatccgtaag tttcattctg gtgcctgagg acctcaacaa
    29161 cagcagattt ctgtatgaaa ccaaagctga aacggattcg gtattcagga gcatccaggc
    29221 caccaaacgt tccagctggt ggcaggatga cggcaagctg ttaatcaacg gcagcaagac
    29281 tcttgccgtc agttttagcg atgaacaggc cttcgaacta aaacagtcgc tttacgtgaa
    29341 tctcagcggt gaactggcag agggagtaaa catctcggcc cagctatccg acagccagtc
    29401 aaagctatct cccgaaggcg attccagaga gctcagcagc ctggaccagg tatttatcaa
    29461 ggtatatgga aaacgctggg aactggcgat gggagacatc gagctcaagt atctgggctc
    29521 cagatacatg gattacagca gccgctttga ggggatcaat gctacaatcg gtgcggacag
    29581 ctatttgcag gccgcttact ctgccggagg gggcaaaccc agccggattg atatcagcgt
    29641 aattgatggc aaacaaggcc cctactacct caatccgggt ggccagccag gcagcctgat
    29701 tgtggtgccg ggcagtgaaa acgtgtatag aaacggtacc aggctggagc ggggagccga
    29761 ttataatatc gactattcag agggaaccct catgttcagg attcttgttt acgccaccga
    29821 cacgatctcg gcctggtttt cctatagtga tgaaaactac cccagacaca gcattttgag
    29881 ctcatccaat ctgagcatca ctcccaggtg gcaggtctcg caccgtgtta tcagcgcttc
    29941 ggatgccaaa gataatcctc tcttgtttca attttctccg gcagatctgg attcactgca
    30001 agcggctgga gattccgatg cctggggctc tggggccacc cttgtggagg cgggtcaggg
    30061 actttacagg ctgttaagca cccccggtgg aatcagttat tatcaatatg ccgagagcga
    30121 cagcctggca aattacctgg tgattttcag ctatgttggc ccccagttgg gtgattatga
    30181 gcagttctcc agcggaaagt atcgctatct gggtcccgga ttgggagaat ggctgcctca
    30241 aaaacgtttg gtttcaccca atagctccgt caacgcgtct ctcagatcag cttacagcta
    30301 tgggagttgg gacctgggcg cggaagccgt gctggccgtc aatgacgcca atacgctttc
    30361 cgggctggac gatgatgata atcaaggtgg atatctctat agctggctta actataaaaa
    30421 cgcgcgctcc agatggcggc ccgagctatt gctggattat gaaaagcgtt gggcacacac
    30481 agttctactc agcgatttca gtgcttccat cagcgattac gacttgggca gtgccgtggt
    30541 cccggattct ttggagttgg attcatatgg tctcaaattg ggtgtgaacc ttggtaagag
    30601 cttcacgtcg gagcttagtg tcaggcattt acgctccgat gacgatttgg agcaaagtat
    30661 cctgaggttc agatcatcgt caccggcctg gggcttattc cccagattgg atataaggtc
    30721 taccctggca aggacatcgg cttccttaga ggggctgggg acgggagaca gcaattacct
    30781 ctttatcgac ggcgcttgga gcgcggcctg gctcaaatgg ggaggcctga taaatcttca
    30841 ggaaaacgca gacatttctc aggacttcaa aaccagcttc ctgagattga atccctacgg
    30901 cgaaatcggt aactccaaaa gcgtccagac cagattggcc ttctctgccg atcagaacaa
    30961 gattgtggac cactctgtct caaatagcca gagctcacag acctggagct tcaaacaact
    31021 ggtcaatacc accaatcaca acctcaatct ggactacaac cacaggcgac ttaacctggc
    31081 cgcggattcc acccgtacca gctacgacct gattaatctg agggccaacg acagctttct
    31141 gaaacgggct atcaccctga tcagcaatta ccagctcaat caaactgagt tctttccccg
    31201 tatccgtgaa cttcagtatg tgggcagcgg actgggggtt tacgacagca ccggagtggc
    31261 cgttcccgaa ggcgattggg actatgagta tatcacatcg tccaccggga ccatgagttc
    31321 agagagaaac gctcagacca gtatctatct gaaacccgga aacctttggg aaaatgacat
    31381 cttacgaagg atcaacaccg atatcgtgat ctccggtaca gaacaacagg aaggccgcca
    31441 aaccgatctg ctcagctatc tcttctggcc gggcagggta tttaatctgg agaacaccat
    31501 ctacggcagg cagagctttc aacaaaacat ctggctggaa ttacggcgca acaagataat
    31561 tgcccagttg gtcttagacc tcgagcgttc tctggataac cgttatcagg acctctccag
    31621 gacctatcgg aggtccgggg ccctgaaact ggatttcaac cgcgtgggac cctacagctt
    31681 cagcatcacg gctgaaaaca aacgcgacag cgacagccgc tatcactctg atgtaaacaa
    31741 ctttgccctg agctcattcg cgcagagaga actggggaaa aacagcatcc tgaggctgga
    31801 cctccgctat tttgaggagc agggcagttc tcagctcaat tcagacagct atggtctcta
    31861 tggcgtggct ctaagtccat ccttccgcag tgtttggggt tccaaaggca ggctgagtgg
    31921 gggattgaac cttcaataca accaacgcga aggtgacgat ctcctcagct ttcttcccga
    31981 aaaaaggggc ggcgctatca ttggcctgaa cctgagcgcc atctatcgcc tcaacgcttt
    32041 cagcaccgga aacattgact atacggcaac atcctatccg ggagaggata tctcccacac
    32101 tctgagaatt gaatttaagg cggaattgta aatggacact ggcataatca ccgcgaacgg
    32161 caccctggac tatctggtgg tgctcccctt tatctttctg gcgggattga tcgattccat
    32221 tgccggaggc ggtggattga tctccctgcc ggcctattgg agtattggta tccctcccca
    32281 cacggccctt ggcaccaaca agttttcatc ctgttgcggc acggtttttt ccaccgccag
    32341 gtatttccat gccggcatga ttgatatccc tgtggcagtc tggagcgcgg tggcggccct
    32401 cttgggatca tggctgggag ccagagtggc gttgatggtt tcggccgctt ttctgaacta
    32461 catgctggta atactgattc ccgggatgac gctattgact cttttgagaa aggacattgg
    32521 ccagaaagac cattccgcca ggctgaaact gggatacagg ataggtctgg gactcgtcgc
    32581 cggtctggtg atcggttttt atgacggctt ttttggtcct ggcaccggca cctttctgat
    32641 cctcatatat gccgtgctgt tgaaatatga ctttgtggta gccaatgcca acaccaaagt
    32701 agtcaatctg gcctcgaatg tggcggcagt cgttaccttt gcagtggcgg gcaagatcct
    32761 ctacgccatc gccattccgg gagccctttg cggcatagct ggaaacctcc tgggagctcg
    32821 tctggtgatc aaaaaaggca accggctgat ccgcgtgatc tttatcctgg ccctgatcct
    32881 gttgctgggc agagtattga gcaatctgct ggccagacta taagacgtct gtggcaaagt
    32941 tgattcaacc atcaacctct gggcgtgtct aatgacaata aaaggcttga cgaaaactgc
    33001 cgtcaaattc caatgtcctc agacgaccat tgatcttcaa aggagattta catgcgtaaa
    33061 atagctttcc tgatgatagt cctgcttatg gcttgcagtg cggctttagc ccaggagcaa
    33121 gttcccaagc aaaccctttg ggccaaacag ttccccgcca cagacctcaa tctggatgtc
    33181 cggtattcca cagaactgaa acgtctttat attcaggtgg acactcaggt ggccaaaacc
    33241 gccatcgaca attttgccct gaacggattc tttctaccgc gggacgctcg gattgtgggc
    33301 ctatggatca acggaaaacc cagcagaatt ctctttgtag gaaagctcca gccgggaaac
    33361 ttcaatcccg ccatcacttc cgccgatctg ctcagcgata actccccagc ccgctatcac
    33421 gtgatggcgc tgaacggtta tgctgaatat cccgacatgg taaaagtaaa cttgtcatac
    33481 tatctggata tgcccgattt tcagcccaac agcctcaacc agatgtatac tctcctcaag
    33541 cctgataaat tctggtatcc cagaaacatc aacgttccca ccaacgtcaa gatcaaagtg
    33601 gtcactacca cttatatgcg tctcatgctg ggagacgcgc tggttccctt taccgacaag
    33661 gactacatgc gggaacacgt agccagtttc agagacgatc ccgccgagcc tctttctttc
    33721 cgtttgacgc gagaataaca ggctaaatta gctcagccgg tagagcaact gattcgtaat
    33781 cagtaggtcg ggggttcgat tcccccattt agctccattg ataaatccgc cctgtcagag
    33841 ctgtctcgag acgcttatcg gcagggcgga tgatttttgt cctgtgtatc actggttcct
    33901 cagctggcac ctcgcacgtt cggcatcgaa tgcgtaaagg cttactataa tatacgcatg
    33961 gcgtcattgg cttgcaccga ctgaagtcag ttcaacgacc ggctgaagag cttgtgcgaa
    34021 aactgattat cgtggcatca ttcctta
//
LOCUS       DAOSFN010000010        30716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1150_length_30716_cov_8.679006, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000010 DAOSFN010000000
VERSION     DAOSFN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 30716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30716
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1150_length_30716_cov_8.679006"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLO35_01570"
     CDS             complement(<1..724)
                     /locus_tag="PLO35_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOH78379.1"
                     /translation="MKYMFKFPDIGEGLEEGKIIEWYVDKGQSVSSGDPLVKMETDKV
                     VTDIPSPKSGVISQRFGAVGETVHVGNALVEIDIEGVEGTAAIKAAAEVPAPPSAKVV
                     EEKGFGVVGTIEVAGDGAYLPSSGEGLQTQAPVSHSDRARKVLATPVARAMAKDLGVD
                     INLLSGSGPAGRVTKKDVEKAAHQSNPPAPSQATLAIPTGSGVRYEPLSMIRKTIAKN
                     MTRSKQNAVHMSVMDEVEVTELV"
     gene            complement(735..1712)
                     /locus_tag="PLO35_01575"
     CDS             complement(735..1712)
                     /locus_tag="PLO35_01575"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOH78380.1"
                     /translation="MKVMNMVQAINDALDLKLQEDKGVVVYGEDVGVEGGVFRVTEGL
                     QLKHGADRVFDSPLAESGIAGTALGMAIAGMKPVIEMQFDGFVYPAMNQILSHISRFR
                     NRTRGKFTVPMVIRIPYGGGINALEHHSESPEAYFGHTPGLKVVIPSTPYDAKGLLIA
                     AIEDPDPVIYMEPKRIYRAIKQEVPEEKFSIPIGKAKVLTPGEHITVIAYGAMIREAQ
                     KAMVIAKEKGISVELIDLRTIWPIDRDTISASVRKTGRALVVTEAPQSYGPAAELVAV
                     INEEAFLSLEAPPVRLTGFDTVIPLPLGEKHYMISPARIAHEIEKIVKF"
     gene            complement(1709..2812)
                     /gene="pdhA"
                     /locus_tag="PLO35_01580"
     CDS             complement(1709..2812)
                     /gene="pdhA"
                     /locus_tag="PLO35_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOH78381.1"
                     /translation="MFDLKTLDSYKPIDDKLYRVMDEKGKIILKDWKPSISDPEILKA
                     YQDLLFERTVDLMAVSYQRQGRMYTYPPNLGQEAIHIAAGKVIQPEDWLVPAFRELGA
                     WLAKGVKLSEIFLYFKGHEDGSKFLNANRMLPVSVPIASQLVHAAGLGYAMNYQNEQA
                     AVFGFVGDGGTSEGDFHEALNFASVWNAPVVFVVQNNQFAISVPLKMQTRSINLAVKG
                     VAYDVPSVMVDGNDFFAMHEVMSFARQYVLENKRPFLIEARTYRIGAHTTSDDPTRYR
                     SKEEEEIWAQRDPAKRLQAWLVKHKLWDPALEEELTAQFRKQIDAEFEAAENYPEYKL
                     DDVFGYMYETLPDELKRQKNNYQNYLKTQGATQ"
     gene            3021..3626
                     /locus_tag="PLO35_01585"
     CDS             3021..3626
                     /locus_tag="PLO35_01585"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOH78382.1"
                     /translation="MKYFRKLSGEKCFLSPISMEDVERYTEWVNDPEIAPFMLFSNTV
                     IGLETERDILKRLSSTDIIFAIVEKDTNKAIGNCGLHNLNEIHRRASFGIFIGEKTFW
                     NQGIGLEATQLILDFGFNILNLNSIDLEVMEYNRRGIRCYEKAGFKYAGRRREHFFHA
                     GKYHDVLIYDIMASEFTSPVIKRSFEHSISDEAGRAKITIV"
     gene            3629..4741
                     /locus_tag="PLO35_01590"
     CDS             3629..4741
                     /locus_tag="PLO35_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOH78383.1"
                     /translation="MFRQIQGQDHAIQILKTAIEHDRVAQAYLFHGPDGVGKFITALY
                     FGMALNCLSKSEYRPCGVCTSCRKLLNFDHPDFIYLFPTPNLQMSVDGEIKDKESLAS
                     YQAYIQNKKATPWQQFRFNKATEIRKESVMMLIRRLDLSIYEANYRICIIEEADTMNI
                     QTANAFLKTLEEPPRNTVMILTTSRLSNLLPTIVSRCQPVYFKPLTRSVTEHLLRERF
                     DYDLPTSRGAARIAGGNFETAVTVAEGSSLGLRDTAFKMMSLAKDDRELEYLNLLESI
                     KEATNPESIRHLFGYLGFIANDLAVLRSNPEEITNVDRIEELNQLARVDDELAGDVLT
                     FLNILEDYRRKIEGNVNLRLILVNNYYALRDLMRGC"
     gene            4746..6308
                     /gene="murJ"
                     /locus_tag="PLO35_01595"
     CDS             4746..6308
                     /gene="murJ"
                     /locus_tag="PLO35_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOH78384.1"
                     /translation="MSQKKLLRNISMMSSAVFLSRVIGLVRDTVMAYFFGTTKLNDAF
                     IVAYKIPNLLRALFGEGALSTAFVPLYNEIGIKRGHRAQMEFALQVVSILCFILSILT
                     MLGLILAPLIVRMLYPGLAESTKLLAIDLSRIMLPYLFLIGLSSTLIAILNSHDYFFM
                     TGLSSALLNIGMLSTIFIPWLLLKPDKETLVYWAAWGVFVGGIFQTLINFPYLRKVGY
                     NFKVLISFRGEAISAMWKRFIPSMIGIGVREINVIADSLMASFLAVGSITALEFGNRL
                     IHMPMGIFAVSTGTALLPLYSRHMTQRNFGELSQSLRFSAITLAYVMLPITMLIMLLS
                     DDFVYLLFQRGAFDARASLMTQQALSFYGIGLIFYSLNQTITPLFYAARDTKTPVKIA
                     AYIVSLNIVLNFILMQFMQHRGLALSTSVTAAINYNVLLWQIRKRLPEVSFSGIWPNI
                     IKAALLCLFLYMPLRWLKDAWSADGKGFILIRDGVFTAVVFVSFYFLGMAIKLPYLRE
                     SANGIWRKLARK"
     gene            6284..8122
                     /gene="uvrC"
                     /locus_tag="PLO35_01600"
     CDS             6284..8122
                     /gene="uvrC"
                     /locus_tag="PLO35_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOH78385.1"
                     /translation="MEKTSPQIEKKVAFLPEQPGIYIWKNAAGEVIYVGKALSLKNRI
                     KSYLTGSHDIKTEQLVKHIADLDYIITNSENEAFLLEATLIKRYRPKYNINLKDDKRY
                     PFVKVTLQDPFPKIMVTRELLKDGAKYFGPFTDGRSLRSTLRDLEWIFTLRTCSRTIP
                     AEGVRFTKACINYQLGKCPAPCIGRIDVAGYGKIVKRVLAFFGGRYQEVADEIRAEML
                     SLSEELKFEQAAKARDRLLAIERIQKRQTVFYTDGRNADIIGCYLEENTAFCVVMRMQ
                     NGAIVNQESYPMANVEHSDEAQVLAAFLKLYYTDHEELPQEIMLPTQPADYEELNIWL
                     KGKLLLPQRGERSRLLAMAKRNAFHLVEERKLAHLRKSSRTVYPIQELKERLELPKLP
                     RKMVCMDISTIQGSDTVSSAVFFENGKPKKKLYRHFIIRSIDTQNDFAALQETFTRFL
                     TEIDKDPDMKPDLFVIDGGKGQLSSCQSILAVSQYPDIPIISLAKRAEEVYVPKASDS
                     IILPRSSSALRLLTSIRDEAHRFAITFHRSRRSRRTLVSELEAIKGVGEKTKFLLLKE
                     LGSVANIAEASVETMISVKGVGAKTAQVVYDYFHSSITDQDMPEEL"
     gene            8508..9131
                     /locus_tag="PLO35_01605"
     CDS             8508..9131
                     /locus_tag="PLO35_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH78386.1"
                     /translation="MYLYPKPRKKTVMPVDYKQKILVAAIKVFAQKGYMNTTMSDVSI
                     QAKVGIGTLYNYFRNKDDLLLSCMRKTIEDEIQHLKDLSDKEIDPMDKLHTFFVHHAE
                     LIRSKPYIAKFLVVELRQSENFYKRNPSYNPLNYYIDFVRDIFKEAMASGRVKKLDVD
                     SLAFMVVGSMDMVMTQWLISGKTLDVVPIIDNIRQILKEGITPDNYA"
     gene            9141..10166
                     /locus_tag="PLO35_01610"
     CDS             9141..10166
                     /locus_tag="PLO35_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78387.1"
                     /translation="MPSNSVRLFIFTVFLWGMATVSSLALDIPVFARAATSNQSSWYP
                     DREGRSLDWDEKYLFAVGIDSLRQADFGLDLEIRAEERLDSFHPVIHTFLLSWVKNSH
                     SLELGTQNIGLASAYDFARRFVAYHAYDGWLFEATRLNAVGYGYHHGFLDITARLGGN
                     QISRGIALLGIGLTPYNQEISAGIRATVSDSHWHSPSLISNLRLKLDLDAFSHNTDLM
                     MKQVLPHNDRPAREEYALASEIAYRFSPRFSFVAGGLYEEREFAPRRISEFHTALDWS
                     FGKLTLSPCFSSKTLTDSREDSYGALLRWAPLPQVRCDLVYKYETGPYDENRHVFAVQ
                     TDLRFDF"
     gene            10126..10860
                     /locus_tag="PLO35_01615"
     CDS             10126..10860
                     /locus_tag="PLO35_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOH78388.1"
                     /translation="MSLPFRQICVSIFSLLLLSCSAGREDQPATIVCLTFDDAHASVY
                     HQALPILERYGLRASNFVNSALLDGSETMTWDQVVSLSKDYGWETGAHGLSHVNLAEL
                     SYPEARQQILSDRQLLVDHGLNPRSFALPYGVCPIDYYDLILPYFDNLRGSNDIPMHS
                     PVDRLNLGYLPFQSGWDAQRVKARISRGVANREALVIIGFHRIATPGEGYADNCPAAG
                     LEEICRWLRDSGLEVMTISEAMAALH"
     gene            10927..12123
                     /locus_tag="PLO35_01620"
     CDS             10927..12123
                     /locus_tag="PLO35_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOH78389.1"
                     /translation="MHLSQRAQEIQASPIRKLMPHAVAAKKRGIKVYHLNIGQPDIPT
                     PKEMIDVYHSFSDKVLAYGPSQGLDVYREGLVQYYHNCGIELNENQIIVTTAGSEAVT
                     FAMYVACEAGDEIIVPEPFYTNYNGFATMAGIRIKALTTYAEDGFKLPDDASIEALIG
                     PKTRAIMVCNPGNPTGTVYSSAEIYRLCALAKKHDLFVISDEVYREFIYGDLTHTSVM
                     HVPGMEELAIMVDSVSKRYSACGARIGCIASRNQEFMAATLKFAQARLCPPTVDQLAA
                     NACVNLGEDFFGPLISEYQHRRDLVFAELQKIPGIVCVQPQGAFYIIAKLPIVDAEDF
                     VIWLLNNFDIDGETVMAAPAEGFYATPGLGRNELRLAYILNEKDLAAAMNIFRRGFDE
                     YKRLHG"
     gene            12196..13128
                     /locus_tag="PLO35_01625"
     CDS             12196..13128
                     /locus_tag="PLO35_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH78390.1"
                     /translation="MKTQWKSSPYLYILPAFLLLFLFRTIPVIMSLIIGFFKWEITGS
                     GEFIGLENYKNMFADPVFWKSITNTLWFVAFVVPASIVFPLFFATLLNQIKRLKSLFR
                     MMYFLPFVTSLVAVSIVWKIIFSEQAGLANSMLGWIGISPQKWLSESTGIFQLIFSSF
                     GVQLPAWMGGPSQALVAIIIMTIWKGLGYNTIIYLAGLQNIPKDYYEAADIDGASKIK
                     QFFRITIPLVSPTTFYVLLMTTIVSFQAFAQIYLMTDKGGPLDTTKLIVYYIYDRGFD
                     ALNMGYASAVALFLFIIVLALTLIQKRLEKHVHY"
     gene            13141..14127
                     /locus_tag="PLO35_01630"
     CDS             13141..14127
                     /locus_tag="PLO35_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH78391.1"
                     /translation="MNDKLRLGIIYFLLSMFGLFMIIPFIWMISTALMTQSEFNKHDS
                     LFIPKERYHVWETAGEQQRVLFVQAKGDSTVVHVLNEELKIKEEYKTVPSSQVREIVK
                     KPSLSWDNFIKAFNKVPFGIYFANTLYVSFVSLLGVLLTSFLAAYAFARMEFWGKNFF
                     FLLFLSMMMVPEPIYLISSYMMLDKLNWLDTYKALIIPWCVNIFTIFLFRQHFRSLPK
                     ELFDAAMIDGASTFGMLWRIILPLSKPIIATASVFSLIGSWNSFMWPLVMTNRPELRV
                     LQVGLSYFNQEASTQTTLLMAASTFSIVPILILFFLAQKQIIASAAKTGMKD"
     gene            14153..16897
                     /locus_tag="PLO35_01635"
     CDS             14153..16897
                     /locus_tag="PLO35_01635"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOH78392.1"
                     /translation="MSSAKGPVKQRILEQAHKNKGQTGTAYKKPEPRPALDDSIYRPQ
                     VIIAPKTNMMIAWIIFAASLLVYLLTQARTLSFWDSGEYATCMSILGVPHPPGNPFYI
                     VFGRAVIALFGGLVSHAVLAAFISGITSAFAVMLTYLVTVQLVSMLKVKAWEAGFAGA
                     MAALLTAFSFTFWMNAVEAEVYAGLSLFVNLIIWLTLRWVQNSRDMDHQNVLLLIIYL
                     FFLGFCVHQTALQIAPAILFIVIYPLFRDGTAKASFWPKVVGYTVAIIMGYVIFGAIG
                     AGMKVDDFDKWGFAVVVLVLLWVELRDVIDPRVWLLAFGLVIAGVSSHLYLAVRAADR
                     PFINEGHPSSWNMFMDYVLRKQYGETSFFDRRGSFFSDQMGFHFMRYIGWQWFNVETL
                     TRWFSVPANVFKATGSMIVGVLGLFGAFFHARGNKHSFYYFLSIILCTTVLMVFVMNI
                     SDSEVRDRDYFFVVGYNMWAIWMGIGAMGILWILKSQAAKIALAIILGTLPLINLATQ
                     YHVHDRSREFVALDYGINFLNSLEENAIIFTNGDNDTFPIWYAQAVQDPHAVEHMHPA
                     KDVYPDQASQAAVKQAMEYKNKYLKGIRKDVTVANLSLLNTPWYVRQLRDKEGVLFSW
                     SDEEITNLIEIPVPDTLRVSAGAANPQMSFKVAYEETPAWRPRENGYRISDVAVMRII
                     QDNFGKRPIYFAVTCESMIGFDDYVRTEGMVNRVVHTKAGEEQQLDIVRLTQNIDTVY
                     KYRSIKDPRVYKDENMNRLIMNYAAAFANAGSYYAMNKDFKKAWSYASKARDFVVDDL
                     RMIDFYVRYYAGQGQWDKLDNYINRYVFTHTDGAGIYLRYIMGYLNRFDPQMAIKYYE
                     KALRQYPEETLVAQFAVYFAEDYSMREPTAIMLERVRDALAYPVDEYLGYLRQTPQPQ
                     "
     gene            complement(16966..19650)
                     /locus_tag="PLO35_01640"
     CDS             complement(16966..19650)
                     /locus_tag="PLO35_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78393.1"
                     /translation="MTKIKSLFISLLLTLPYLLFAWSGYESLSTPYFKVFYRPGWEDE
                     ALNLLQTMEYSRPFVEDLTGNRLDQIPFVIEDMGNMVNGYTSPTNSKIAVFAYPPSGD
                     ELSNGEDWWQVVGVHEYIHMAQMTRISGEPSLIRSLFGNLFYPNLYQPNWMSEAITVY
                     GESAMSPYSGRMNGGTYPAIISALSAEGKMPSLTKAGYYSSSTPLANHYVFGGSFYQY
                     LADTYGPDKFPILFEYTGGSLFSYLNPLYSNLSLDKAYQDAYGKPLQVLWSEWQAHEA
                     AKKLSFPNQPITHRGHNISQLRYHEGSLYYISYSRDKTGPFSSFGSNRLIRMSDPDAA
                     AREEVLVEQATEFPAGYQIVGDELYYTRQEMVKGYDNNEFDGLGVITEIWKKDLKSGS
                     TRKISYGQIRSFLVRGDGSILTAEDDKTHQNSLLYVLDPVSGGKLQITRLNYLIGSMH
                     EADGKLYVTARGFYRNNSIYELNLGTRQMTPVIDTPYGESIVDVKDGKIIYESVYDGT
                     NGCYSYDIATRAVSRLGDFNELRSLAIAPNGRNYFVSMNSKGQDIYKDPLRLKAFQLP
                     TEQKKAPYQRLEAGKSDLILDKYPPQKGSYLANIGHMLWPRLYRFPYLAGTEDSLQVG
                     LQVAGSDLLGDFPYWGASVIWDSYSDDLGFQIDLENNFFRPLKQTISYSSFNGKSLSA
                     NQYVQLLSKMNYGLTDVWAGVGLVTSEGLDRKLWYPYLGTAFGWRGGSLSATNHLMYE
                     TTDFWASDRDRLGWQARATLKQKLSKASELRSRVHVAWDPEANIDEVFSTIRGYDQTW
                     EQTKGVVWQNSLYTPLIKIREGIWNPNIYLEDIAAGLFFDASIPSNRDEAQTRWAYGA
                     ELIAELYAGYMFSLDLGFRLSYNKDKLLLPSLILGTEF"
     gene            complement(19725..21014)
                     /gene="mtaB"
                     /locus_tag="PLO35_01645"
     CDS             complement(19725..21014)
                     /gene="mtaB"
                     /locus_tag="PLO35_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOH78394.1"
                     /translation="MRLAISTLGCKTNSYESAAIASAFPEGWELVDFSTAADVYIVNT
                     CTVTGRTDFKSRNLIRKALARKAQDPSVKVVVTGCFAQRHPLEVAELGEVDLIVDNQS
                     KADIWDLLNGSANHFRDASEATSFRYAPVTAMLDRSRAFQKVQDGCGLFCAYCAVSHA
                     RGPSRSARLTDVLAQARLFVRSAYREIVLGGVNLGLYRDGETDLTDLLGEMKQIEGLE
                     LIRLSSLEPQLVTQRLLEELASGSIYCPHLHLSLQSGSETVLKRMGRRYGAEDFERLV
                     AQIVTRIPQVAIGADVIVGFPGETDTEFAETRRLVESLPFAYLHVFSYSKRPGTPAAA
                     MPDQIPSFVKKERSNILLNISGRKTADYQKHLLDEHVTLRGVVESVHSGVATMLSDHF
                     VRAYTKDRVEPGSVVAVIPTHIHSDGLMGDLARGNKN"
     gene            complement(21017..21622)
                     /locus_tag="PLO35_01650"
     CDS             complement(21017..21622)
                     /locus_tag="PLO35_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOH78395.1"
                     /translation="MERAASEARRIAVLTGGHGRGSNLKAIHEYFQAKGFPARVELVV
                     GTRQETPVRELCASLGLRFEYIASKSMPEFESQLSHLVIACGIELIALAGFLKLLSAD
                     FLKQVAIPVLNIHPALIPKHCGAGMYGMKVHESVFSAGEKISGATVHEVDALYDHGRI
                     IAQQCIDIGACLTPAEIAARVLQVEHQIYAPAILKRLLGGE"
     gene            complement(21589..23370)
                     /gene="aspS"
                     /locus_tag="PLO35_01655"
     CDS             complement(21589..23370)
                     /gene="aspS"
                     /locus_tag="PLO35_01655"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOH78396.1"
                     /translation="MLDNFANLRRSHRCGELSAALDGQPVTVMGWVHKRRDLGGLIFI
                     DLRDVSGILQIVIHPENTAAFAKAEKLRNEYVAAFEGIVRLRGEGNVNSQLATGEIEV
                     EAGEVFILSDSQPLPIQLNENALPEEDLRLTYRYLDLRRPKLQRVIVTRHKLTQAIRE
                     FLNQEGFLEIETPILMKSTPEGARDYLVPSRVHPGKFYALPQSPQMFKQLLMISGFDR
                     YYQIARCFRDEDLRADRQPEFTQLDVELSFVSMEQIFDLMERLMAHVFRHVLGIELPI
                     PFPRLSYQEAMERFGCDKPDLRFGMELMDLSDIFQNSEFAVFRSALDNGGVVKAIVIP
                     GAHEYSRKQMDALVELAKHQGGKGLAFVKYHEAQLGAGISKFITTAQGEAMMARLGVK
                     EGDLIAFAADSYEMACKILAAVRNQCALELGLIPPNSFAMAWITDFPLFQYNPEARRW
                     EPAHHMFTLPKEEHIPWLDDPKRIGDIHGQLYDMVCNGMELSSGSIRCHRYDVQKRIF
                     DILGFEEQELQDRFGFFLEALKYGTPPHGGIAPGLDRLVMILSDAASIRDVIAFPKTL
                     KAADLMTGAPSEVPEPQWKELHLKPGE"
     gene            complement(23440..24483)
                     /locus_tag="PLO35_01660"
     CDS             complement(23440..24483)
                     /locus_tag="PLO35_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOH78397.1"
                     /translation="MNYATIIVMLLSLLLGSCSKGPVLYVFNWSDYIDPELIAEFEKE
                     YGCKVKYSTYDSNENMLTKIKSSRESFDLVFPSGDHVSIMIENGLAEKLDKAKLTNIA
                     NLDTLLMAKANEFDAGNQYSLPYFWGITGLLYNKQEVPAELVAKQSWNVIGDQFFSGK
                     RKITMLDDAREVVGAAMISNGYDVNDTSEAALAAALTTLQSWDGNITQFDSESYKNEV
                     ADGTTWLAQAYNGDALQQMAQNQDLGFIFPQEGSSLWMDNMVILKSSKNKELAYQFIN
                     FLLRPEVAKRNADYVQYPTPNAAAFELLEASDKENLLIYPDDAYLQKCHMIRFLGDEV
                     TKINDLFEKIKMN"
     gene            complement(24494..25765)
                     /locus_tag="PLO35_01665"
     CDS             complement(24494..25765)
                     /locus_tag="PLO35_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH78398.1"
                     /translation="MKLSRSVWGWMFYDFANSAFTTIIVSVVYSAYFVKSVVAGSEGY
                     GEMLWGRAIGISMTLVALTAPILGAVADHSRAKKRLLFMNCWLTVIFTALLWFVGPGD
                     IFSGMLFFIIANFGFNSANVFYDAFLPEVSSPASVGKISGYGWSFGYLGGLLSLVLSL
                     ILLKVNVRLIFPMIALHHFGFSLFTFFWLKEVRLPSQRTNYLKAGFNRVVWSIRNIRH
                     LPQLTRYILSYFIYNDGITTVIAFASIYGITRFGMNTQDMIIYFILAQFTSIAGAAVF
                     GWLTDKTNTKLALSISLLIWIAVVIWAFFCSSAFEYYLVGLLAGLAIGSSQANSRTML
                     SQLTPPDKQAEFFGFYTLTGRLSAIVGPILYGWIAHQTGDLRWSVLSLMAFFIIGWIL
                     LQSVSLSRGIKEARDLNKSINTSWGASASHN"
     gene            complement(25762..>26139)
                     /locus_tag="PLO35_01670"
     CDS             complement(25762..>26139)
                     /locus_tag="PLO35_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOH78399.1"
                     /translation="DAQMGMLLKLESREKQWQRNLVGAHLDDYDIGLEGRPMKVYASQ
                     GQKRMAVIALKLTQANLVQKVTGVKPLLLFDDIFAELDAFHSGKVRELTDTRDQIFVA
                     SPRPDVARNWPGLEALKGFGEGA"
     assembly_gap    26140..26239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26240..26601)
                     /locus_tag="PLO35_01675"
     CDS             complement(<26240..26601)
                     /locus_tag="PLO35_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH78400.1"
                     /translation="MILQKLELHNYRNYQARVFEPEARGNLIIGPNGCGKTNLLEAIS
                     YCGIGRSVRSHKDDEILAYGEPEWIVRAVFIKDNGSQLEISLHWSEGKKLLKLDGVVA
                     RQLSSLFQNVKTIYCAPE"
     gene            complement(26605..27777)
                     /gene="dnaN"
                     /locus_tag="PLO35_01680"
     CDS             complement(26605..27777)
                     /gene="dnaN"
                     /locus_tag="PLO35_01680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOH78401.1"
                     /translation="MRLAIEKKELLHHIQHLATVVSTKNTSPIMLNYLMEVDEVSSTV
                     KLTASDLELTAKVSFPAAVSEGGAIAISARHFNEIINFMPDAVIDLWKNEELLMIQCN
                     KVDFNILCADPTLYPQLGDPDTADANVMSAELFMRMVAKTSFAVSMDVQRAVLTGVCW
                     KIMKDRHLMAATDGRKVSEIIIKAPSEAASEVASQAEGETIFSEPESEQYLERVIPVK
                     TLSFLQRIYDSSEKEIRISLQHSKMIFSYGRFVVISNIIEQKYPEYRKAFITDLPNKL
                     IVDKKLLNTAIRRVALVAPEDVMRIRFEIDEQRFEINTSDRDTGDAKEFIESYSYQGT
                     PTSIAFNYKYMVSILEAVDTEKVRIFLGSGKDPMMVYNEADPENQEITYLLMPLRS"
     gene            28128..28715
                     /locus_tag="PLO35_01685"
     CDS             28128..28715
                     /locus_tag="PLO35_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH78402.1"
                     /translation="MDVTKRQMDIVDAAIRIIARKGYRELTTKNLARELKLTEAALYR
                     HFRNKDELLQTILTYFGEISAQVLENIRDHGLSPWQKVERFVMNRFEIFCQKPDLGSV
                     IFSEELFRNDPALMEHMRSIMHSHRDEVIIYIKEAQELGQINPHCDPDQIFRIVVGAM
                     RFTVTQWVLSQRGFDLVEEGQKLIKAIKTLTEEYK"
     gene            28712..29524
                     /locus_tag="PLO35_01690"
     CDS             28712..29524
                     /locus_tag="PLO35_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013245087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH78403.1"
                     /translation="MKRQIITIDEALCTGCGNCVTGCHEGALQIIDGKARLISDLFCD
                     GLGACIGTCPYGAITIEEREAEPYDEALVMESMISKGDATIKAHLKHLKDHGEMGFYN
                     QALQILKIKGFDIDALTKEETMACGCSGTHDQKLEPKSSAAVCGDLSSKLRQWPVQLH
                     LLNPAAQYFENSDLLISADCVPYAFGDFHNRFLDGKIVITFCPKLDKDIDRYIDKLAQ
                     IFTLHQIKSVTIVRMEVPCCGGTEIIVKRALEQANKMHFVKVNIISVDGNII"
     gene            29540..29857
                     /locus_tag="PLO35_01695"
     CDS             29540..29857
                     /locus_tag="PLO35_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH78404.1"
                     /translation="MYSRTWKDLEPVLNVNGLHGVKLYSETEGEIVHLKLEPGAHLKA
                     HKTPVNVLFYVLEGEATLHIGDEVATYPKDSTVDSPKDIPHAVTNNGKVDLRLLVIKM
                     PKP"
     gene            29875..>30716
                     /locus_tag="PLO35_01700"
     CDS             29875..>30716
                     /locus_tag="PLO35_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOH78405.1"
                     /translation="MSMFCYQCQETSRNLGCTMRGVCGKSPELCHFMDLSIHAMKGLA
                     HVSKRLLEKGVYHKEDGLFVMQGLFRTITNANWDEEQIKAVITEAIALRDKRKQELCD
                     LLGGDCSTCPPAVTFKVEPQDYEEFGKSVGVLATANEDVRSLRETVIYGLKGIAAYGD
                     HAAMLGYESDEIYQFIMEGLAATIDDSLSADQLTALVIKTGEYAVKVMELLDRANTET
                     YGVPEPTVVKLGVGTNPGILVSGHDLKDFDELLQQSEGQGVDIYTHSEMLPANYYPAF
                     KKYK"
BASE COUNT         7307 a         8176 c         7721 g         7412 t
ORIGIN      
        1 ccacaagttc tgtgacttcc acttcatcca tcacgctcat atgaacggcg ttttgcttgg
       61 aacgggtcat atttttggcg atggtcttgc ggatcatgga gaggggctcg tatctgactc
      121 cggagccagt cggaatcgca agggtggctt gtgatggagc aggcggattg gattgatggg
      181 ccgctttctc cacatccttc ttggtgactc tgccggcggg accgctgccg ctgaggagat
      241 tgatatccac tcccaggtct tttgccatcg ctctggcaac gggcgtggcg aggacttttc
      301 gcgctctatc gctatgcgaa acaggagctt gcgtttgcag tccttcaccg ctggatggca
      361 ggtaagctcc atctccagcc acttcgatcg tgcccaccac gccaaagccc ttttcctcca
      421 ccactttcgc agatggagga gcgggtactt cggcagctgc tttaatggct gcagttcctt
      481 ccacaccttc tatatctatc tccaccaatg cgttacccac gtgaaccgtc tcacccacag
      541 cgccaaagcg ctgggaaatc acgccactct tgggagaagg aatatcggtg accaccttgt
      601 cggtctccat cttgacgagc ggatcgccgg agctcacact ctggccttta tccacatacc
      661 actcgatgat tttaccctct tccaggccct caccgatatc gggaaactta aacatatact
      721 tcatctcgtc ctccctaaaa cttcacgatc ttttcgatct cgtgagcaat gcgggctggc
      781 gagatcatat agtgcttttc acccaagggc agtggtatca cggtatcaaa acctgtcagc
      841 cggacggggg gtgcttccaa agacagaaaa gcttcttcgt tgatcacggc gaccagttcc
      901 gcggcgggac catagctctg aggtgcttcg gtgacaacca gggccctgcc tgtcttgcga
      961 acagaagcgg agatggtgtc tctgtctatg ggccagatag tgcgcagatc gatcaactcc
     1021 accgaaatac ccttttcttt ggctatcacc atggccttct gagcttccct gatcatagct
     1081 ccataggcga tcacggtgat atgctcgccg ggagtgagca ctttggcttt gccgatcggg
     1141 atgctgaatt tctcctctgg tacttcttgt ttgatagccc gatagatgcg tttgggctcc
     1201 atatagatga cggggtcggg atcctctatg gccgctatca gtaaaccttt ggcgtcgtag
     1261 ggagtggagg gaatcactac tttgaggccg ggagtgtggc caaaataggc ttccgggctc
     1321 tcggaatggt gttccagagc gttgatgccg ccgccgtagg gaatccggat caccatgggg
     1381 acggtgaatt tgccccgggt gcggtttctg aaacgggaaa tgtgggataa aatctgattc
     1441 atcgccggat agacaaagcc gtcaaactgc atctcgatca ccggcttcat tcccgcgatc
     1501 gccatcccaa gcgcggtgcc ggctatacca gattccgcca gcggagaatc gaagactctg
     1561 tccgcgccgt gtttgagctg cagaccttcc gtcacccgaa acacgccacc ttccactccc
     1621 acgtcctctc catataccac cacgcctttg tcctcctgga gtttgaggtc gagcgcgtcg
     1681 ttgatggcct gtaccatatt catcactttc attgtgtggc cccctgggtc ttgagatagt
     1741 tctgataatt gtttttctgc cgtttcagct catccggcag ggtttcatac atgtatccga
     1801 agacgtcgtc aagcttatac tcgggatagt tctccgcagc ttcaaattcg gcatcgattt
     1861 gcttgcggaa ttgggctgtg agttcctctt ccagagcggg atcccagagc ttgtgcttca
     1921 ccagccaggc ctggaggcgt ttggcgggat ctcgctgcgc ccagatttcc tcctcctctt
     1981 tgctgcggta gcgggtggga tcgtctgagg tagtgtgagc gccgatcctg taggtcctgg
     2041 cctcgatcag gaagggacgc ttgttttcca gcacatactg tctggcgaag ctcataacct
     2101 catgcatggc aaagaaatcg ttgccgtcca ccatcaccga gggcacgtca taggccacgc
     2161 ctttcactgc aaggttgatg gagcgggtct gcattttgag aggaacggaa atggcaaact
     2221 ggttgttttg caccacaaac accaccggag cgttccagac ggaggcgaag ttgagcgctt
     2281 cgtggaagtc tccttcggag gttccgccgt cgcccacgaa tccaaagacg gcggcctgct
     2341 cgttttggta gttcatggca tagcccaatc cagcagcatg caccagctga gacgcaatcg
     2401 gcacagatac aggcagcatg cgattggcat tcagaaactt ggagccgtct tcatggcctt
     2461 tgaaatagag gaaaatctcg ctgagtttga cgcccttggc cagccaggct cccagttcgc
     2521 gaaaggctgg caccagccag tcttctggtt ggattacctt cccggccgcg atatgaatcg
     2581 cttcttgtcc caaattggga ggataggtat acattcttcc ctgtcgctga taactcactg
     2641 ccatcaggtc cactgtgcgt tcaaacagca agtcctgata ggccttcaat atctcgggat
     2701 cagaaatgga gggcttccag tctttgagaa ttatcttgcc tttctcgtcc atcacccggt
     2761 acagcttgtc atcaatgggt ttatacgaat cgagggtctt aagatcgaac attctaacca
     2821 ccttttgtgc atgtttttga tatcctacct atataatagg ctattctgtt tcggtatgca
     2881 agtatgaatt ttgtggttat caaagaaacg gtggaggcat ggtcgcggcc ttttctcttg
     2941 acagatagag cggcctctgg agtgtgagat aagatgtaaa catcagccac attggtttga
     3001 aactgcaata aggagccagg atgaaatact ttcgcaagct aagcggagaa aaatgtttcc
     3061 tttcacccat atccatggag gatgtggaac gctataccga atgggtcaac gatcctgaga
     3121 tcgcgccatt tatgctgttt tccaatactg tgatcgggct cgagaccgaa cgtgatatat
     3181 tgaagaggct gagcagcacg gacatcatct tcgccatcgt ggagaaggac accaacaaag
     3241 ccatcggcaa ctgcggtctg cacaatctca acgagatcca tcgccgcgct tcctttggta
     3301 tatttatcgg ggaaaagacg ttctggaatc aggggatcgg cctggaagcc acccagctga
     3361 tcctggattt tggctttaac atcctcaacc tgaacagtat agacctggaa gtaatggaat
     3421 ataaccgtcg cggtatccgc tgctatgaga aagctggttt taaatatgcc ggccgcagac
     3481 gcgagcactt ttttcacgcg gggaaatatc acgacgtgct gatctacgac atcatggcca
     3541 gcgaattcac cagccctgtc atcaagcgtt cctttgaaca cagcatcagc gacgaagccg
     3601 ggcgcgccaa gatcacgata gtgtagaaat gtttcgccag attcaaggcc aggatcacgc
     3661 tattcagatc ctgaagacgg caattgagca tgacagagtg gctcaggcct atctttttca
     3721 cggcccggac ggagtgggta aatttatcac cgccctctat tttgggatgg cgctgaattg
     3781 tttatccaag agtgaatacc gtccttgcgg agtatgtact tcctgccgca aactgctcaa
     3841 ctttgatcat ccggacttca tctacctgtt ccccacaccc aatcttcaga tgagtgtgga
     3901 tggcgagatc aaagacaaag agagcctggc cagctaccag gcttacattc aaaacaaaaa
     3961 agccactccc tggcagcagt tcagatttaa caaagccacc gagatccgca aggaaagcgt
     4021 gatgatgctg atccgcaggc tggacctctc tatctatgag gccaactacc ggatatgtat
     4081 tattgaagag gccgacacta tgaacattca gactgccaat gcctttctca aaacattgga
     4141 agagccaccc cgcaacactg tgatgatcct caccaccagc cgcttatcca atctgctgcc
     4201 caccatcgta tcgcggtgcc aaccagtcta tttcaaaccg ctcacccgtt cagtaacgga
     4261 acaccttttg agggagcgct ttgactatga tctgcccact tccagagggg ctgccagaat
     4321 cgcgggcggg aattttgaga ctgcggtcac ggtcgcggaa ggaagctccc tgggccttag
     4381 agacacagct ttcaagatga tgtccctggc aaaagacgat cgcgaattgg agtatctaaa
     4441 cctcctggaa agcatcaaag aggccaccaa ccccgaatcc atccggcacc tgtttggcta
     4501 ccttggattt attgccaatg acctggccgt cctgagatcc aatccggaag agatcaccaa
     4561 tgtggacaga atcgaggaac tcaatcagct ggcccgcgtg gatgatgaac tcgcgggtga
     4621 cgtgctgacc tttttgaata tcctggaaga ttatcgccgc aagatcgagg ggaacgtgaa
     4681 tctgagactg atcctggtga acaactacta cgcgctcaga gatctgatga gaggctgtta
     4741 gcctgatgtc ccaaaaaaag ctgctgcgca acatcagcat gatgtcctcc gcggtgtttc
     4801 tgagccgcgt gattgggttg gtgcgggata ccgtgatggc ctatttcttt ggtactacca
     4861 agctaaacga tgctttcatc gtggcctaca agattcccaa tctcctcaga gcgctgtttg
     4921 gcgaaggagc tctttccacg gcttttgtac cgctctacaa cgagatcggg atcaagagag
     4981 gccaccgtgc ccagatggaa ttcgctctgc aggtagtctc catcctctgc tttatcctct
     5041 caatactcac aatgctgggc ttgattttag cgcctttgat cgtgagaatg ctctaccccg
     5101 ggctggcaga gagcaccaag ctgctggcca tcgatctttc caggatcatg ctgccctacc
     5161 tgtttctgat cggtctctcc tccaccctga tcgccatcct caattcccac gactatttct
     5221 tcatgaccgg cctctcatcc gcgttgctca atatcggcat gctgagcacc atcttcatcc
     5281 cctggctctt gctcaaacca gataaggaaa cactggttta ttgggccgcc tggggtgttt
     5341 ttgtgggagg gatcttccag acccttatca atttccccta cctgcgcaaa gtcggttaca
     5401 atttcaaggt gctgatcagc ttcaggggag aagccataag cgcgatgtgg aagagattta
     5461 tcccctcgat gattggcatc ggcgtgagag agatcaacgt gatcgcggac agcctgatgg
     5521 cctcgtttct ggctgtgggc agcatcacgg cgctggaatt tggcaaccgg ctcatccata
     5581 tgccgatggg catcttcgct gtctcgactg gaacagccct tctgccgctt tattccaggc
     5641 acatgacgca gcgcaatttt ggcgagctct cccagagcct ccgtttcagc gctatcacgc
     5701 tggcctatgt aatgttgccg attacgatgc tgatcatgct cctgagcgac gactttgtct
     5761 atctgctctt ccagcgaggc gctttcgatg cccgcgccag tctgatgact cagcaggcac
     5821 tctcttttta cggcatcggt ctgatctttt acagcctcaa ccaaaccatt acccccctct
     5881 tctatgccgc ccgggatacc aaaacgcctg tgaagatcgc cgcttacatc gtgagcctca
     5941 acatcgtgtt aaactttatc ctcatgcagt ttatgcagca tcggggcctg gctctctcca
     6001 cctctgttac agcggcaatc aactacaacg tcctgctctg gcaaatccgt aaacgccttc
     6061 cggaagtgag ctttagcggc atttggccca atatcatcaa agccgctttg ctctgcctgt
     6121 tcctgtacat gccgcttaga tggctgaaag atgcctggag cgcggatggg aaaggattta
     6181 tcctgatcag ggatggagta ttcaccgcgg tcgtttttgt ttccttctac tttttgggaa
     6241 tggccatcaa gctcccttat ctgagagaga gcgcgaacgg gatatggaga aaactagccc
     6301 gcaaatagaa aagaaggtag cgttcctgcc tgaacaaccc ggcatctata tctggaaaaa
     6361 cgctgccggg gaagtgatct atgttggcaa ggccctctct ctgaaaaacc gcatcaaatc
     6421 atatctgacc ggatctcacg acatcaagac ggagcagctg gtaaaacaca tcgccgatct
     6481 ggattatatc atcaccaaca gcgaaaatga ggccttcctg ctggaagcga ccctgatcaa
     6541 gcgttaccgt cccaaataca acatcaacct caaagacgac aaacgttatc ctttcgtgaa
     6601 agtcactctg caggatccgt ttccgaagat tatggtgacc cgtgagctgc tcaaggatgg
     6661 cgcaaaatac tttggccctt tcacggacgg gcggtcttta cgctccactc tgagggacct
     6721 ggagtggata tttaccctcc gcacctgctc ccggaccatt ccagccgagg gcgtgagatt
     6781 cactaaagcc tgtatcaact atcaattggg caaatgtccc gccccctgta ttggcaggat
     6841 cgatgtagcc gggtatggaa aaatcgtcaa acgggtgctg gcattcttcg gaggcaggta
     6901 tcaagaggtg gcggatgaaa tcagggctga gatgctgagc ctgagcgaag agctcaagtt
     6961 tgagcaggcc gccaaggccc gggaccgtct gctggccatc gagcgcattc aaaaacgcca
     7021 gactgtcttt tacacggatg gccgcaacgc ggacatcatc ggctgctatc tggaagaaaa
     7081 cacagccttt tgcgtggtga tgaggatgca aaacggcgcg atcgtcaacc aggaaagcta
     7141 ccccatggcc aatgtggaac acagcgacga agcacaggtg ctggcagcct ttctaaagct
     7201 ctactacacc gatcacgagg agctgccaca ggagatcatg ctgccaaccc agccagccga
     7261 ttatgaagag ctcaacatct ggctgaaggg caagctgcta ttgcctcaaa ggggtgaacg
     7321 cagccgcctc ctggccatgg caaaacgcaa cgccttccat ctggtggaag aacgcaaact
     7381 ggcccatcta cgcaagtcca gccgcaccgt ctatcctatc caggaactca aggagcggct
     7441 cgaacttccc aagctgccgc ggaagatggt ctgtatggat atctctacca tccagggtag
     7501 cgacaccgtc tcttctgccg tctttttcga aaacggcaaa cccaagaaga agctctaccg
     7561 ccatttcatc atccgcagta tcgacaccca aaacgacttt gccgccctgc aggaaacctt
     7621 cacccgtttc ctcaccgaga tcgacaaaga tcctgatatg aaaccggatc tgtttgtgat
     7681 cgacggcggt aaaggacagc tcagttcctg ccagagtatc ctggcggtat ctcaatatcc
     7741 ggatatcccg atcatttctc tggccaagcg ggccgaggaa gtatatgtcc ccaaagcgtc
     7801 tgactccatc atcctgccac gttcctcgtc agctttgaga ctgctgacct cgatccgcga
     7861 cgaagctcac cgctttgcca tcacttttca ccggtcgcgc cgctcccgac gcaccctggt
     7921 gagcgaactg gaagccataa aaggggtagg cgaaaagacc aaatttctgc tgctcaaaga
     7981 gctgggttcg gtggccaaca tcgctgaagc cagcgtcgaa accatgatca gcgtgaaagg
     8041 agtgggagcc aagaccgcgc aagtggtcta tgattatttt cactccagta tcacggacca
     8101 ggatatgcca gaagagcttt aagcggcata cttttagcgc ttgactgagc aactggaatg
     8161 aaagcgctcc ctctgagaag ttgcatccca tcacgagcat agagggccat gattacaaaa
     8221 ctcactcttc tgaggacacc tgccacctca atccttgctt attaccccgc cgtggagatg
     8281 ccgtgcgctc tccggcgaat ttcggcggag agcgcacggt atctccacgg cgggtgatta
     8341 tgcaagcaac cagacatgag aagcggaaca agatttgcat gttaaattga tcaccacccg
     8401 gatatgacgt gtttggcagt cccggcagcg ttaatatcct tgacaagatt atacagagct
     8461 taaaaagtgg attcaaaaca ataattatga taaaataaga ggagaatatg tatctctacc
     8521 cgaaacctcg caagaagact gtgatgcctg ttgattacaa gcagaaaatc ctcgttgccg
     8581 ccatcaaagt ttttgcccag aaaggctata tgaacaccac catgtcggat gtgtccatcc
     8641 aggccaaagt gggtattgga acgctctaca actatttccg taacaaggac gatctgctgc
     8701 tgagctgcat gagaaagacc atcgaagatg aaatccagca tctgaaagat ctcagtgaca
     8761 aagaaatcga tccgatggac aagctgcaca ccttttttgt tcaccatgcc gaactgatca
     8821 ggagcaaacc ttacatcgcc aagtttctgg tagtggagct gcggcagagc gaaaactttt
     8881 ataagcgcaa tcccagttac aaccccctga actactacat agacttcgtg cgcgatatct
     8941 ttaaggaagc tatggccagc gggcgcgtca aaaaacttga cgtcgacagc ctggcgttta
     9001 tggtggttgg atctatggat atggtgatga cccagtggct gatcagtggt aagactctgg
     9061 atgttgtgcc cattattgac aatatccgtc agatcctcaa agagggtatc actcccgata
     9121 actacgcgta aagggtattg atgccaagta atagcgtcag gctgtttatc ttcacggtct
     9181 tcctttgggg aatggccacg gtaagcagcc tggcgcttga catacccgtt ttcgcccgcg
     9241 ccgccaccag caatcagagt tcctggtatc cagacagaga aggccgctct ttggactggg
     9301 atgagaagta tctgttcgcg gtggggatag acagtttgcg ccaggcagat tttggtctcg
     9361 atctggaaat cagggcagaa gaacggctgg acagcttcca cccggtcatc catacatttc
     9421 ttctcagctg ggtaaaaaac tcccactctc tggagctggg cacccaaaac attggcctgg
     9481 cttccgctta tgactttgcc agacgctttg tggcatatca cgcttatgat ggctggctgt
     9541 ttgaggccac ccgcctcaat gctgtgggat acggctatca tcatggcttt cttgatatta
     9601 cagccaggct gggaggcaac cagatctcac gcggaatcgc tttgctgggc atcggactca
     9661 ccccatacaa ccaggaaatc agtgccggga tcagagctac cgtctctgac agccactggc
     9721 attccccctc gctgatttca aacctaaggc taaagctgga tttagatgcc ttcagccaca
     9781 acacagatct gatgatgaaa caagtgttgc ctcataacga cagacctgcc cgcgaggagt
     9841 acgctctagc gtctgaaatc gcctataggt tctcaccccg tttcagtttt gtcgcgggag
     9901 gcttgtatga agagcgggaa ttcgctcccc gccgcatcag tgagtttcac accgcgctgg
     9961 actggagctt tggcaaactg acgctttcac cctgcttttc cagtaaaacg cttaccgatt
    10021 cccgggaaga tagttatgga gcactgctgc gctgggcccc tcttccacaa gtccgctgtg
    10081 acctggtcta taaatatgaa accggaccct atgatgagaa ccgccatgtc tttgccgttc
    10141 agacagatct gcgtttcgat ttttagcctg ctgctactat cctgctcagc cggcagagaa
    10201 gatcagcccg ccaccatcgt ctgcctgact ttcgatgacg cgcacgccag cgtctatcac
    10261 caagctctgc caattctgga gcgatatggc ctcagggcca gcaattttgt aaacagcgct
    10321 ctgctggatg gttctgagac catgacctgg gaccaggttg tcagtctgag taaagactat
    10381 ggctgggaaa ccggagcgca tggactttcg cacgtaaacc tggcggaact cagttacccc
    10441 gaggcgcgac agcagatatt gtctgacagg caactgcttg tggaccatgg tcttaatcct
    10501 cgcagttttg ctctgcccta tggcgtttgt ccgatcgatt attacgattt gatcctcccg
    10561 tattttgaca acctccgcgg tagcaacgac atccccatgc acagccccgt cgataggctc
    10621 aatctgggct atttgccctt tcagagtgga tgggacgcgc agcgcgtcaa agccaggata
    10681 tccagaggag tggcaaaccg cgaagccctt gtcatcatag gctttcacag aatcgcgacc
    10741 ccgggagagg gatatgcgga caactgtcct gccgccgggc tggaagaaat ctgtcgctgg
    10801 ttgagagatt caggtctgga agttatgacc atttcagaag cgatggctgc attgcactaa
    10861 agattttgct tgtctgaatt cagccatata gaaattgtgg aacaaagaca attttgtgag
    10921 gataaaatgc acttatcgca acgcgcccag gaaatccagg cgtctcccat ccgcaaactt
    10981 atgccccacg cggtagctgc caagaaacgt gggatcaaag tataccacct caatatcggt
    11041 cagccggaca ttcccactcc caaagagatg atcgatgtat atcacagctt ttcagacaag
    11101 gtcctggcct acggtccctc tcagggactg gacgtctatc gcgaaggttt ggtgcaatac
    11161 taccataact gcggcatcga actgaatgag aatcagatca tcgtcaccac cgctggcagt
    11221 gaagctgtca cttttgccat gtatgtggct tgtgaagccg gcgacgagat catcgtacct
    11281 gagcctttct atacgaatta caacggtttt gccaccatgg ccggaatcag gatcaaagcc
    11341 ctcaccacct atgccgaaga tggttttaag ctgcctgacg atgccagcat cgaagctctg
    11401 atcggcccta aaaccagagc catcatggtc tgcaatcctg gcaacccgac cggcaccgtt
    11461 tatagtagcg cggagattta caggctttgc gccttggcca aaaagcatga tctgtttgtg
    11521 atctcggacg aggtctatcg tgagtttatc tacggcgacc tcacccacac cagcgtgatg
    11581 catgtgcctg gcatggaaga acttgccatc atggtagaca gtgtgtccaa acgctacagt
    11641 gcctgcgggg ccagaatcgg ctgcatagct tcccgcaatc aggaattcat ggctgccaca
    11701 ctgaaattcg cccaggcaag gctttgtcct cccacggtcg atcaattggc tgccaatgcc
    11761 tgtgtgaacc tgggcgagga cttctttggg cccctcatct ctgaatatca gcaccggcgt
    11821 gatctggttt tcgctgagct gcaaaagatc cctggaatcg tctgcgtcca gcctcagggc
    11881 gctttttaca tcatcgccaa gctgcccatt gtggacgccg aggactttgt gatctggctg
    11941 ttaaacaact ttgatatcga cggcgaaacc gtgatggcgg cccccgcgga aggcttttat
    12001 gccactcccg gtctgggcag aaacgagctt cgcctggcct atattttgaa tgagaaggac
    12061 cttgccgcgg ccatgaatat cttccgtcgc ggatttgatg aatacaagcg gctccatggc
    12121 tgatttttcc gttgacagag ctatgctgtc caagcaatcc cgctccggcg ggaatgatct
    12181 tttcaaggcg gttttatgaa gactcagtgg aagtcctcgc cctacctgta tatcttgccg
    12241 gccttcctgt tacttttcct gttcaggact atccccgtga tcatgtctct gatcatcggg
    12301 ttctttaaat gggagatcac tggcagcggt gagtttatcg gtctcgagaa ctataagaac
    12361 atgttcgcgg acccggtgtt ttggaaatcc atcaccaata ccctctggtt tgtggcgttt
    12421 gtagtgccgg caagcattgt ctttccgctc tttttcgcca ctctgctcaa tcagatcaag
    12481 aggctgaaga gccttttcag gatgatgtat tttttgcctt ttgtcacgtc gcttgtggca
    12541 gtttcgattg tgtggaagat catcttctcc gagcaggccg gcctggccaa cagcatgctg
    12601 ggatggattg gaatatcgcc acagaaatgg ctgtctgaaa gcaccgggat cttccagctg
    12661 atcttttctt cctttggggt tcaattgcct gcctggatgg gagggccttc ccaggcactg
    12721 gtggccatca tcattatgac catctggaaa ggcttgggat acaataccat catctatctg
    12781 gccggacttc agaatatccc caaagattac tacgaggctg ccgatatcga cggcgccagc
    12841 aagatcaagc agttcttccg gatcacgatc ccgctggtgt cacccaccac cttctatgtg
    12901 ctgctcatga ccacgattgt cagcttccag gctttcgccc agatctatct gatgaccgat
    12961 aaaggtggcc cgctcgatac caccaaactg atcgtctatt acatttacga ccgtggattc
    13021 gacgctctga atatgggtta cgccagcgcg gttgccctct tcctgtttat catcgtgctg
    13081 gccctcacgc ttatccagaa acgtctggaa aagcatgttc actattgatg ggaggcatga
    13141 atgaacgata aactgcgcct cggcatcatc tacttcctgc tttccatgtt tgggcttttc
    13201 atgatcatac cttttatctg gatgatctct accgccctga tgacccagtc ggaatttaac
    13261 aaacacgatt ccctctttat ccccaaagag cgttatcacg tatgggaaac cgctggggaa
    13321 cagcaacggg ttctgtttgt acaggccaaa ggtgacagca ccgttgtcca cgtcctcaac
    13381 gaagagctta agatcaaaga ggaatacaag actgtaccca gctctcaggt gagggagata
    13441 gttaaaaaac ctagccttag ctgggacaac tttataaaag cgtttaacaa agttccgttt
    13501 ggcatatatt ttgccaatac cttatatgta tcttttgtca gcctgctggg ggtgctgctc
    13561 acttccttcc tggctgccta tgcttttgcc agaatggagt tttggggcaa gaacttcttt
    13621 ttcctgctgt ttctcagcat gatgatggtg cccgaaccta tttacctgat ctcatcatac
    13681 atgatgctgg acaagctcaa ctggctggat acctataagg ccctgatcat cccctggtgc
    13741 gtgaatatct tcactatctt cctgttccgg cagcatttcc gctccctgcc aaaggagctg
    13801 tttgacgccg ccatgatcga cggggccagc acctttggca tgctctggag gatcatcctg
    13861 ccactttcca agcccatcat cgctaccgct tcggtattct cactgatcgg ttcctggaac
    13921 agctttatgt ggccattggt gatgaccaac cgacccgaac tgagagtctt gcaggtgggc
    13981 ctcagctatt tcaatcagga ggcttccacg cagaccacct tgttgatggc ggcctctacc
    14041 tttagcatcg tacctattct tatccttttc ttcttggccc agaaacagat cattgccagt
    14101 gccgccaaaa ccggtatgaa ggattgataa acttttataa tggagtagat taatgagttc
    14161 agccaaaggc ccggtaaagc agagaatcct tgaacaggcc cataagaaca agggacagac
    14221 cggtaccgct tacaaaaagc cggaaccgcg tccagctctt gatgacagca tataccgtcc
    14281 tcaggtgatt attgccccca agaccaatat gatgatcgcc tggatcattt tcgcggcctc
    14341 gctgttggtg tatcttctca ctcaagcacg gaccctatcc ttctgggaca gtggagaata
    14401 cgccacatgt atgagcatcc tgggagtgcc tcatcctcct ggaaacccgt tttacattgt
    14461 cttcggcagg gcggtgatcg cgttgtttgg aggtctggtc agccatgccg tgttggcagc
    14521 gtttatctca ggtatcactt ccgcttttgc cgtcatgctc acataccttg ttaccgttca
    14581 attggttagc atgctcaaag tcaaagcctg ggaagcgggt ttcgcggggg ccatggcagc
    14641 tttgctcacc gctttttcct ttactttctg gatgaacgcg gtggaggctg aagtctatgc
    14701 cgggctgtct ctgttcgtca atctgatcat ctggctcaca ctgagatggg tgcaaaactc
    14761 ccgtgatatg gaccatcaaa acgtgctgct gctgatcatc tatctcttct ttctgggttt
    14821 ttgcgtgcac cagacggccc tgcagattgc tcccgccatc ctcttcatcg tgatttatcc
    14881 gctctttcgg gacggaacgg ccaaggccag tttctggccc aaggtggtgg gctacaccgt
    14941 agcgattatc atgggatacg tgatttttgg ggctatcggc gcgggaatga aagtagatga
    15001 ttttgacaaa tggggtttcg cggtcgtggt tttggtcctg ctctgggtgg aactcaggga
    15061 cgtgatcgac ccccgggttt ggctgctggc ttttggacta gtgatcgcgg gtgtatcctc
    15121 gcatctgtat ctggctgtga gggcggcaga ccgtcccttt atcaatgaag gtcatcccag
    15181 cagctggaac atgtttatgg actacgtgct gcgcaagcag tatggagaga cctcattctt
    15241 tgacaggcgg gggtcttttt tcagcgatca gatgggcttt catttcatgc gctacattgg
    15301 atggcagtgg tttaacgtag agaccctcac tcgttggttc agcgttccgg ccaacgtgtt
    15361 caaagccact ggatcgatga tagtgggtgt gcttggcctg tttggagcgt tcttccatgc
    15421 ccgcggcaac aaacacagtt tctactattt tctttccatc attctctgca ccacagtatt
    15481 gatggtgttt gtgatgaaca tttcagattc ggaagtgaga gaccgcgatt atttctttgt
    15541 ggtgggctac aacatgtggg ccatctggat gggaatcggc gccatgggaa tcctgtggat
    15601 attgaaatcc caggctgcca aaatcgccct ggccatcatc ctgggaaccc ttcctctgat
    15661 caatcttgcc acccaatatc acgtgcatga tcgctcccgt gagtttgtgg ctctggatta
    15721 tgggatcaac ttcctgaatt ctctggaaga aaacgccatt atctttacca acggcgacaa
    15781 cgacaccttc cccatctggt atgcccaggc cgttcaggac ccccacgccg tggagcacat
    15841 gcatcccgct aaagacgtct atccggatca ggccagccag gctgccgtca aacaggccat
    15901 ggagtataaa aacaagtatc tcaaaggcat ccgcaaagac gtcactgtgg ccaatctgtc
    15961 gcttctaaac acaccctggt atgtgcgcca gttgcgagac aaggaaggag tgctgtttag
    16021 ctggagcgat gaagagatca ccaatctcat cgaaattccg gtaccggaca ccttgcgtgt
    16081 gagtgctggg gcggccaatc cccaaatgag cttcaaagtc gcttatgaag aaaccccggc
    16141 ctggagaccc cgcgaaaacg gctatcgcat ctccgatgta gcagtgatga gaatcattca
    16201 ggacaacttt ggcaagcgcc ccatttattt tgccgtcact tgcgagagca tgatcggttt
    16261 tgacgactat gtccgcacgg aagggatggt aaatcgggtg gtgcacacca aggccggcga
    16321 ggaacagcag ttagatatcg tccgactgac gcaaaacatc gataccgtat acaaataccg
    16381 ctctatcaaa gaccccaggg tatacaaaga cgaaaacatg aaccgcctga tcatgaatta
    16441 cgcggccgcc tttgccaatg ccggatcata ttacgccatg aacaaagatt tcaagaaggc
    16501 ctggtcctac gcctccaaag cacgcgactt tgtggtagac gacctcagga tgatcgattt
    16561 ctacgtgcgt tattatgccg gtcagggaca atgggacaag ctggataact atatcaaccg
    16621 ctatgtgttt acccataccg atggggcagg tatctacctc agatacatca tgggatatct
    16681 gaaccgcttt gatccccaga tggcgatcaa atactacgag aaagccttaa gacagtatcc
    16741 tgaggaaacc cttgtagcgc agtttgccgt ttacttcgcg gaggactata gcatgcggga
    16801 gccaacagcc attatgctgg aaagggtccg ggatgctctg gcttaccctg tggacgagta
    16861 tttaggatac ctcaggcaaa ctccccagcc tcaataagtt atccggcagc aatatcgaaa
    16921 gcgccctcag gatttgaggg cgctttcatg taaactggat ttagtttaga actcggtgcc
    16981 cagtatcagt gagggcaaca gcagcttgtc tttattatag ctgaggcgaa accccagatc
    17041 caggctaaac atataaccgg catagagttc agctataagt tcagctccgt aggcccagcg
    17101 ggtttgggcc tcatcccggt tggaaggaat ggaggcgtca aagaaaagcc cggctgcgat
    17161 gtcttccaga tagatattgg gattccagat cccctctctg atcttaatca aaggagtgta
    17221 gagggagttt tgccagacga cccccttggt ctgttcccac gtctggtcgt aaccgcgaat
    17281 ggtggaaaac acctcatcga tattggcttc agggtcccag gccacatgga ccctggaacg
    17341 cagttcggaa gctttgctca gtttctgctt caaggttgcc cgtgcctgcc agccaaggcg
    17401 atcgcggtct gaagcccaga aatcggtggt ctcatacatc agatggttgg tggccgagag
    17461 cgatccccct ctccatccaa aggccgtgcc cagataggga taccaaagct tgcggtccag
    17521 gccttccgaa gtcacgagtc ccactcccgc ccagacatct gtgaggccgt agttcatctt
    17581 gctgagcagc tgcacatatt gattggcgct taagctttta ccattgaagc tggaatatga
    17641 gatggtctgt ttgagaggcc ggaaaaagtt gttttccaga tctatctgaa agcccaaatc
    17701 gtcgctgtaa ctatcccaaa tcacagaggc tccccaatag ggaaaatcgc ccagaaggtc
    17761 tgatccggct acctgcaatc ccacctgcaa gctgtcttcg gtgccggcca gatacggaaa
    17821 gcgatacaaa cgcggccata gcatatgacc gatattggcc agataactgc ccttttgagg
    17881 tgggtattta tccaggatca aatcgctttt acctgcttcc aatctctggt agggggcttt
    17941 cttttgctcc gtgggcaatt ggaacgcttt caagcggaga gggtctttat agatatcctg
    18001 gccctttgag ttcatggaaa caaagtagtt acggccgttg ggagctattg ccagagaccg
    18061 cagctcgttg aaatctccca gtctggagac ggcgcgggtg gcgatgtcat aggaataaca
    18121 gccattcgtg ccatcatata cggattcata gatgatcttg ccgtctttca catcgacgat
    18181 actttctccg tagggggtgt cgatcacggg ggtcatttgg cgggtgccca gattcagttc
    18241 gtagatactg ttgttgcggt aaaaccctcg tgccgttaca tatagcttgc catcggcttc
    18301 atgcatgctg ccaatcaggt agttgagcct ggtgatctgc agtttaccgc ccgaaacggg
    18361 gtccaggacg tagagcaggg agttttgatg ggtcttgtcg tcttccgcgg tcagaatact
    18421 gccatctcct ctcaccagaa aagatctgat ctgtccatag ctaattttgc gggtgctgcc
    18481 agatttgagg tcctttttcc agatttcggt gatcactccc agaccgtcga actcgttgtt
    18541 gtcgtagcct ttcaccattt cctggcgggt atagtaaagc tcatcgccca cgatctgata
    18601 accggcggga aactcggtgg cttgttctac cagcacttct tctctggccg cggcgtcggg
    18661 gtcactcatc ctgatcaacc ggttggagcc aaaactgctg aagggaccgg ttttatccct
    18721 ggaataactg atgtaataaa gagagccctc atgatagcgg agctgggaga tattgtggcc
    18781 tcgatgggtg attggctgat tgggaaaact gagctttttg gcagcctcgt gggcctgcca
    18841 ttccgaccaa aggacctgca gcggttttcc ataagcatcc tgataggctt tgtccagcga
    18901 gagattggaa tacagaggat tcaggtagga aaacagcgac ccgcctgtgt actcaaagag
    18961 gatgggaaac ttatctggtc cataggtatc ggcaagatac tgatagaaac tgcctccaaa
    19021 aacatagtga ttggccaggg gagtagaaga gctgtaatat cccgctttgg tcagcgaggg
    19081 catctttccc tccgcggaaa gagctgagat gatagcagga taagtacccc cgttcatgcg
    19141 tccactataa ggtgacatgg cggattcgcc atacacggtg atcgcctcac tcatccagtt
    19201 gggctgatag agattgggat aaaacaagtt accaaacaaa gagcggatga gggagggctc
    19261 gccactgatc ctggtcatct gagccatgtg gatatattca tgcactccca ccacttgcca
    19321 ccagtcctcg ccgttggaga gctcatcgcc gctgggtgga taggcaaaga cggcgatctt
    19381 gctgttggtg ggactggtat aaccgtttac catgtttccc atgtcctcaa tcacgaaggg
    19441 aatctggtcc agacggttgc cggtcaggtc ttccacgaaa ggacggctgt attccatcgt
    19501 ttgcaataga ttgagggctt catcctccca gccgggacga taaaacacct tgaaatatgg
    19561 cgtgctgagg ctctcatagc cggaccaggc aaatagcagg taaggcagcg tgagaagcag
    19621 cgagataaac agtgatttga tcttcgtcat gataactccg actcgggtat tgtagtggtc
    19681 gtttgatgat aaactaatca ggttttggtg atggttatca ttctttagtt tttattgcct
    19741 ctggccaggt cgcccatcaa tccatcggaa tgaatgtgag tgggaatcac agcaaccacg
    19801 ctgccgggtt ccacgcgatc tttggtatag gctctgacaa agtggtcaga cagcatcgtc
    19861 gcgactccgg aatggacgga ttccaccact cctctgaggg ttacgtgttc atccaataga
    19921 tgcttttggt agtccgcggt cttgcggccg gatatattga gcaggatgtt ggagcgctct
    19981 tttttcacaa aagagggtat ttgatcaggc attgcagccg cgggagttcc tggccgtttg
    20041 gagtagctga agacgtgcag ataagcaaag ggcagcgatt ccaccaatct tcgcgtttca
    20101 gcgaactccg tgtctgtctc gcctggaaag cccacaatca catccgcccc gatggcgacc
    20161 tggggtatcc gcgtcacgat ctgtgctaca agccgctcaa agtcttcagc tccatagcgg
    20221 cgtcccattc ttttcaatac cgtttcagag cccgactgca acgacagatg caaatgagga
    20281 caatagatgc tgccggaagc cagctcctcc agcaatctct gcgtcaccag ctggggctcc
    20341 agcgatgaca gcctgataag ttccagtccc tcaatctgtt tcatttctcc gagtaagtca
    20401 gtcagatcgg tttcgccgtc ccgatacagc cccagattca cacctcccag cacaatctct
    20461 ctataagcgc tgcgtacaaa gaggcgcgcc tgagcaagca cgtctgtcag tctggccgat
    20521 ctggaaggtc ctcgggcgtg tgatacagcg caataggcgc aaaacagacc acagccgtcc
    20581 tgaactttct gaaaagccct gctgcggtcc agcatggcag tgacgggcgc gtagcgaaag
    20641 ctggtggcct ctgaggcgtc tctgaagtgg tttgctgagc cattcagaag atcccagata
    20701 tcggctttgc tctgattatc aacaatcagg tccacttctc ccagttccgc cacttccaat
    20761 ggatggcgct gggcaaaaca accggttacc accaccttga cggatgggtc ttgcgccttg
    20821 cgggctaaag ctttgcggat caggttgcgg ctcttgaaat cggtgcggcc ggtcacggtg
    20881 caggtgttga cgatatagac gtccgccgct gtggaaaaat ctaccagttc ccagccctct
    20941 ggaaacgctg aagcgatggc agcagattca taggaattgg tcttgcagcc cagagtggag
    21001 atcgccagcc gcatgattat tcaccaccca gcaagcgttt caggatggcg ggagcgtaga
    21061 tttgatgctc cacttgcagc acccgggcgg caatctctgc gggtgtgagg caagcgccga
    21121 tgtcaatgca ctgctgagca ataatcctgc cgtgatcgta gagcgcgtcc acttcgtgca
    21181 cggtcgctcc gctgatcttt tcaccggcgg aaaaaacgct ttcatgcact ttcatcccat
    21241 acatgccagc cccgcagtgt tttggaatca gggcaggatg gatgttcagc accggaatgg
    21301 ccacctgctt gagaaaatca gccgaaagca gtttcaggaa tcccgccagc gcgatcaatt
    21361 caatcccgca tgcaataacc agatgggaca gttgcgactc aaattcaggc atagactttg
    21421 aagcaatgta ttcaaagcgc aagcccagcg aagcgcaaag ctctctgacc ggtgtctcct
    21481 gacgtgttcc caccaccagt tccacccggg ctggaaatcc cttagcctgg aaatactcat
    21541 gaatggcctt aaggttggat ccccgtccat ggccaccagt cagaacggct attcgcctgg
    21601 cttcagatgc agctctttcc attgcggctc agggacttca gagggcgcgc cagtcatcag
    21661 atcagcggct ttaagggttt tggggaaggc gatgacgtca cggatggaag cggcatcact
    21721 gaggatcatc accagtctgt ccagtccagg tgctatgccg ccatgaggag gggtgccgta
    21781 tttgagcgct tccagaaaaa agccaaagcg gtcctggagc tcctgttctt caaatccaag
    21841 gatgtcgaag atccttttct gcacatcata acgatggcat ctgatgctgc cggaggatag
    21901 ctccatcccg ttgcagacca tgtcataaag ctggccatgg atatctccga tccgcttggg
    21961 atcatcaagc cagggaatat gttcttcttt cggcagagta aacatgtgat gggctggttc
    22021 ccagcgccgg gcttcaggat tatactgaaa gagcggaaaa tccgtgatcc aggccatggc
    22081 aaagctgttg ggaggaatca gtccaagctc cagggcgcat tgattgcgca ccgctgccag
    22141 gattttgcag gccatctcat agctgtcagc ggcaaaagcg atcagatcac cttctttgac
    22201 acccagtctg gccatcatgg cttccccctg tgcggttgtg atgaacttgc tgattccggc
    22261 accaagctgc gcttcatggt atttcacgaa ggccagtcct ttgccgcctt ggtgcttagc
    22321 aagttctacc agggcgtcca tctgcttacg gctgtattca tgggcaccag gaattacgat
    22381 cgctttgacc actcccccat tgtcaagagc ggaacggaac accgcgaatt cggaattctg
    22441 gaagatatcg ctgaggtcca taagctccat gccaaaacgc aggtccggct tatcgcagcc
    22501 aaaacgctcc atggcttcct gatagctgag ccgcgggaag ggaatcggca gctcgatccc
    22561 gagcacatgt ctaaacacat gcgccatgag gcgttccatc aggtcgaaga tctgctccat
    22621 cgagacaaag ctcagctcca catccagctg ggtaaattca ggctggcgat ccgctctcag
    22681 gtcttcatct ctaaagcagc gggcgatctg ataatagcgg tcaaaaccgg atatcatcag
    22741 aagctgtttg aacatctgag ggctctgtgg aagggcgtaa aacttgccgg gatgcactct
    22801 gctgggcacc agataatccc tcgccccttc cggagtgctc ttcatgagga tgggagtctc
    22861 gatctcaaga aagccttcct ggtttagaaa ctcacggatc gcctgagtca gtttgtgacg
    22921 ggtgacaatc actctctgga gtttggggcg tcgcaggtcc aggtatctgt aggtgaggcg
    22981 caagtcctct tccggcaagg cattttcgtt taactgaatg ggcagaggct gagaatctga
    23041 gaggataaac acttctcccg cttccacttc tatctctcca gtggccagtt gggaattgac
    23101 attcccctct ccccgcagac gcactatgcc ctcaaaagcc gctacatact cgtttctgag
    23161 tttctcagct ttggcaaaag ccgcggtgtt ttcaggatgg atgacgatct gtaggattcc
    23221 gctgacgtct ctcagatcaa tgaaaatcaa gccacccaaa tcgcggcgct tatgcaccca
    23281 gcccatcaca gtgaccggct ggccgtccag agccgctgaa agctctccgc aacgatggct
    23341 gcggcgcaga ttggcaaagt tgtctaacat tctaattcct tttcaaccgg gataaggtcc
    23401 tttttttatg tttaatcgca tcatagagac acgcgtcctc tagttcatct tgatcttttc
    23461 aaacaggtcg ttgatcttgg tgacctcatc acccaaaaac cggatcatgt ggcatttttg
    23521 gagataggca tcgtccggat aaatgaggag gttttctttg tcgctggctt ccagaagctc
    23581 aaaagcagcc gcgttggggg tgggatactg cacataatcg gcattgcgtt tcgccacttc
    23641 cggacggagc aggaaattga tgaattgata ggcaagctct ttgtttttgg atgatttgag
    23701 gatcaccatg ttatccatcc acaagctgga accttcctgc gggaaaatga atcccaggtc
    23761 ctggttctgg gccatctgct gtaaagcgtc tccattgtag gcttgggcga gccaggtggt
    23821 cccatccgcc acttcgtttt tataggactc gctgtcgaac tgggtgatgt tgccatccca
    23881 gctttgcaga gtggtcagag ctgccgccag agccgcttca ctggtgtcgt tgacgtcgta
    23941 accgttgctg atcattgccg cgcccaccac ttctctggcg tcatccagca tggtgatctt
    24001 acgcttgccg gaaaagaact ggtctccgat cacgttccag ctctgttttg ccaccaactc
    24061 ggccgggacc tcctgtttgt tataaagaag gccggtgatt ccccaaaaat acggcagaga
    24121 gtattgattg cccgcgtcaa actcattggc tttggccatc aacagcgtgt ccagattggc
    24181 gatattcgtg agttttgcct tatcaagctt ctcggccagt ccgttctcga tcatgatgga
    24241 aacatgatct ccgctgggaa acacgaggtc aaatgactct cggctgctct tgatcttggt
    24301 gagcatgttt tcgttggaat cgtaggtgct gtacttcacc ttgcagccgt attctttttc
    24361 aaactcggca atcagttccg gatcgatata atcgctccag ttaaaaacat agagcaccgg
    24421 gcccttggaa caggaaccca gcagcaggga cagcagcata acgataattg tggcgtaatt
    24481 catgtaaact cctttagttg tgggaagcgc tggctcccca ggatgtattt attgacttgt
    24541 tgaggtcccg cgcttctttg atgccgcggg agagggaaac gctttgcaac agaatccatc
    24601 cgatgataaa aaaggccatc agggagagta ccgaccagcg caaatctcca gtctgatgag
    24661 caatccagcc atacaggatc ggccccacaa tggcagacag ccgtcctgtc agggtataaa
    24721 atccaaagaa ctccgcttgt ttgtccggag gagtaagctg cgacagcatc gtgcggctgt
    24781 tagcctgaga gctgccaatc gccagcccgg ccaggagacc caccaaataa tactcgaaag
    24841 cgcttgagca aaagaatgcc catatcacta ccgcaatcca gatcaaaaga gagatagaga
    24901 gcgccagctt ggtgttggtt ttatcggtca gccacccaaa aacggccgcc cccgcgatag
    24961 acgtaaactg cgccaatata aagtagataa tcatatcctg ggtattcatc ccaaagcggg
    25021 tgataccata aatggacgcg aaagcgatca cagtggtgat cccgtcgtta tagatgaaat
    25081 agctgaggat atagcgcgtc agttgtggca gatgtctgat gttccgaatc gaccagacca
    25141 cccggttgaa cccagccttg agatagttgg tgcgttggga tggcagccgc acttccttga
    25201 gccagaaaaa ggtaaatagc gaaaagccaa agtgatgcag cgctatcatt ggaaagatca
    25261 gccgcacgtt cacttttaga aggatcagag acaagaccag cgaaaggagg cctcccagat
    25321 atccaaacga ccaaccgtag ccggaaattt tgcccacgct tgcaggagag ctcacttccg
    25381 gcagaaaagc atcataaaac acatttgcgc tgttaaatcc aaaattggct ataatgaaga
    25441 acagcatgcc ggaaaagata tctcccggtc ccacaaacca aagtagagca gtgaagatca
    25501 ccgtgagcca gcaattcata aacagcaagc gctttttggc cctggagtga tccgctacag
    25561 cgcctaaaat cggggctgtg agcgctacca gcgtcattga tatccctatc gctcttcccc
    25621 acagcatttc gccataaccc tcagagccag caaccacgct cttgacaaaa tacgcgctgt
    25681 aaacaactga tacgataata gtcgtaaatg ccgaattggc aaagtcataa aacatccagc
    25741 cccatacgga gcggctcagc ttcacgcgcc ttccccaaat cccttcaggg cttccaaacc
    25801 aggccagttg cgtgctacat caggtctggg gctggccacg aaaatctggt cccgggtgtc
    25861 tgtgagttct ctcaccttac cgctgtgaaa ggcatccagc tcggcaaaaa tatcatcgaa
    25921 caagagtaag ggtttcactc ccgttacttt ttgcaccaga ttggcttgcg tgagttttag
    25981 cgcgatcacc gccatccgtt tttgcccctg ggaagcgtaa accttcatcg gtcggccctc
    26041 aaggccgatg tcataatcat ccagatgcgc gcctaccaga tttcgctgcc actgcttttc
    26101 cctggattcc agcttgagga gcatgcccat ctgagcatcn nnnnnnnnnn nnnnnnnnnn
    26161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26221 nnnnnnnnnn nnnnnnnnnt cctcgggagc gcagtagatg gtcttcacat tctgaaacag
    26281 agagctaagc tgccttgcca caacgccatc cagtttgagc agtttcttgc cctcgctcca
    26341 gtgcaggctg atctccagct gcgagccgtt gtccttgata aacacggctc tcacgatcca
    26401 ttccggctcc ccataggcca ggatctcatc gtctttgtgc gatctcacgc tacggccaat
    26461 accgcaataa gaaatagcct ccaggaggtt ggtcttgcca caaccgttgg gacctattat
    26521 caggtttcct ctggcctccg gttcaaacac ccgggcctga taattacgat agttgtggag
    26581 ctcgagtttt tgaaggatca atcactaaga ccgcagaggc ataagcaggt aagtgatttc
    26641 ctggttttca ggatcggctt cattataaac catcatgggg tctttgcctg aacccaggaa
    26701 aattctcact ttttcagtgt cgaccgcttc caggatggac accatatatt tatagttgaa
    26761 cgcgatactg gtgggtgtac cctgatagct gtaggactcg ataaattctt tggcgtcacc
    26821 ggtatcgcgg tcgctggtgt tgatctcaaa gcgctgctca tcaatctcga aacggatccg
    26881 catcacgtct tccggggcca ccaaagccac ccgacggata gcggtgttga gcagtttctt
    26941 atccacaatc agcttgttgg gcaggtcggt aatgaaagct ttgcgatact cgggatattt
    27001 ctgctctatg atgttggaga tcaccacaaa acgtccatag gagaagatca tcttgctgtg
    27061 ctggaggctg atccggattt ccttttcaga ggaatcgtag atccgctgca gaaaactgag
    27121 ggtcttgacg gggatcactc tctccaggta ctgctcactt tccggctcgg agaaaatcgt
    27181 ctcgccctcc gcctggctgg caacttcgga agcagcttca ctgggggcct tgatgatgat
    27241 ctcagagacc ttgcggccat cggtggcagc catcaaatga cggtctttca tgatcttcca
    27301 gcagacgccg gtcaaaacgg cccgctgcac atccatggac acggcaaaag aggtcttggc
    27361 caccatcctc ataaagagct cggcgctcat cacattggcg tccgcggtat ccggatcgcc
    27421 caactgggga tagagggtgg ggtccgcgca caagatattg aaatctacct tgttgcattg
    27481 gatcatcaga agttcttcgt ttttccatag atcgatcacc gcgtccggca tgaagttgat
    27541 gatctcattg aaatgcctgg cgctgatggc gatagcaccg ccttcgctga ccgcggctgg
    27601 aaagctgacc ttggcagtaa gctccagatc ggaggctgtc agtttgacgg tactgctaac
    27661 ttcatccact tccatcaagt agttcagcat aatgggagag gtgttcttgg tcgataccac
    27721 ggtggccagg tgctggatgt ggtgcagaag ctcttttttc tctatggcga gtctcatgtt
    27781 tacccctgta aaatatgatg ttatatgtga tgatcaaatt cctgcaccag ccgttttatg
    27841 tcaatgactt tatagtggct ccgatctata aatcgaaagc agacaagaaa caagctcttt
    27901 cgctctgtga tccatgatat atctcccaag ctctcaagct gccagaggcc ataaacatag
    27961 cgtccgccca cccttcccaa aaataattgc atatcataaa ggtatgatat ttagtcactt
    28021 cgtaaacctg cccctttttt ttcgcaatat acagcggatt tttcttgcca aaaatgccgc
    28081 cctgaatagt ttggttagtg aatacttact aacccaagga agagatcatg gatgtgacta
    28141 aacgacagat ggatatcgtg gacgcggcga tccgcatcat agcccgcaag gggtatcggg
    28201 agctgaccac caagaatctg gccagggagc tcaaactcac tgaggcagcc ctctatcgcc
    28261 acttcagaaa caaagacgag ctcctgcaga ccatcctgac ctattttgga gagattagcg
    28321 cgcaagtcct tgagaatatc cgtgatcatg gtctcagtcc ctggcagaaa gtggagcgct
    28381 ttgtcatgaa ccgtttcgag attttttgtc agaagccgga cctcggcagc gtgattttct
    28441 cagaggagct gttcagaaac gatcccgctt tgatggaaca catgcgttct atcatgcact
    28501 cccatcgcga cgaggtgatt atctatatca aagaagctca ggagctggga cagattaatc
    28561 cccattgcga tccggaccag attttccgga tcgtcgtggg cgcgatgcgc ttcaccgtca
    28621 cccaatgggt cttgagccag cgtggctttg atcttgtgga agaaggccaa aagctgatta
    28681 aagcaataaa aacacttaca gaggaataca aatgaaaaga cagatcatca cgattgacga
    28741 agcactctgc accggctgcg gcaactgcgt taccggttgt catgagggag ctttgcaaat
    28801 cattgatggc aaagcccgcc tcatcagcga tctcttctgc gatgggctgg gggcctgcat
    28861 cggcacctgc ccttatggcg ccatcacgat cgaagaacgg gaagccgaac cctacgacga
    28921 agccctggtg atggaaagta tgatctccaa aggcgatgcc accatcaaag cccacctcaa
    28981 acacctcaaa gatcacggcg agatgggatt ctacaatcag gcactccaga ttctcaagat
    29041 aaaaggcttc gacatcgatg ccctcaccaa agaagaaacc atggcctgcg gctgcagcgg
    29101 cacccatgac cagaaactgg agcccaagag cagcgctgcc gtctgtgggg acctcagttc
    29161 caaattgcgg cagtggccag tccaactcca tctgctcaat cctgccgctc agtactttga
    29221 aaattctgac cttttaatca gcgccgattg cgtgccctac gcttttgggg attttcacaa
    29281 ccgcttcctg gatggcaaga tagtcattac tttctgcccc aaactggaca aggatatcga
    29341 cagatatatt gacaaacttg cccagatctt tactctgcac cagatcaaat ccgtcaccat
    29401 cgtccgcatg gaagtgccct gctgcggtgg tacagagatc atagtgaaac gtgctctgga
    29461 acaagccaat aagatgcact ttgtgaaagt caacatcatc tctgtagatg gaaatatcat
    29521 ttaaacaagg agatagataa tgtacagccg cacctggaaa gacctcgaac cggtactcaa
    29581 cgtcaacggc ctgcacggcg tcaagcttta ctccgaaacc gagggcgaaa tcgtgcatct
    29641 caagctggag cctggggctc acctcaaggc ccacaagact cccgtcaatg tactatttta
    29701 tgttctggaa ggcgaagcca ctctccatat cggagacgaa gtagccacct atcccaaaga
    29761 cagcaccgtc gacagcccca aagacatccc ccacgccgtc accaacaacg gcaaggtgga
    29821 cctgcgtcta ttagtgatca aaatgcctaa accataataa aaaggagcaa aaacatgagc
    29881 atgttctgtt atcagtgtca ggaaacctcc cgcaacctcg gttgcacgat gcgcggcgtc
    29941 tgtggaaagt cgcccgagct gtgtcatttt atggacctat ccatccacgc catgaagggc
    30001 ttggcccacg tttccaaacg tctgctggag aaaggcgtct atcacaagga agacggtctt
    30061 ttcgtgatgc agggcctctt tcgcactatc accaatgcca actgggatga agaacagatc
    30121 aaagctgtca tcaccgaggc gattgccttg cgtgacaagc gtaagcaaga actctgcgac
    30181 cttctgggtg gcgattgcag cacctgcccc ccagccgtca ccttcaaggt cgaaccccag
    30241 gactatgaag agtttggcaa aagcgtgggt gtgctggcaa ccgccaatga agacgtccgt
    30301 tcactgcgtg agaccgtgat ctatggcctc aagggcatcg ctgcctatgg cgatcatgcc
    30361 gccatgctcg gttacgaaag tgacgagatc tatcagttta tcatggaagg cctggccgcc
    30421 accatcgacg acagcctttc cgccgaccag ctcaccgctt tggtgattaa aaccggcgaa
    30481 tacgctgtca aagtgatgga actgctggac cgcgccaaca cagaaaccta tggcgtcccg
    30541 gaacctacag tcgtaaaact gggcgtgggc accaaccccg gtatcctcgt cagcggacac
    30601 gacctcaagg actttgatga gcttctgcag cagtccgaag gccagggcgt ggacatctac
    30661 actcacagcg aaatgctgcc agccaactac tatcccgcct tcaagaaata caaaca
//
LOCUS       DAOSFN010000011        29066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1245_length_29066_cov_8.954845, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000011 DAOSFN010000000
VERSION     DAOSFN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 29066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29066
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1245_length_29066_cov_8.954845"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            445..1050
                     /gene="infC"
                     /locus_tag="PLO35_01705"
     CDS             445..1050
                     /gene="infC"
                     /locus_tag="PLO35_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOH78406.1"
                     /translation="MPKERINGQITSSNVRLIGADGRQVGVVSIREALRRAEEQDLDL
                     VEISPNVDPPVCRITDFSKYYFQKEKKAKEARKKQHEVEVKEIKYGPNTEDHDYGFKK
                     NNAIKFLKQHNKVKFTVRFRGRQMAHKELGFNLLERIKADLAHIADVDAEPVSDRNLL
                     FLVMSPKKEIDKILGGEKKATPVAEEAVPAVPETVEPKPES"
     gene            1182..1376
                     /gene="rpmI"
                     /locus_tag="PLO35_01710"
     CDS             1182..1376
                     /gene="rpmI"
                     /locus_tag="PLO35_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOH78407.1"
                     /translation="MPKIKTNRSAAKRFKVTGSGKIVRHHAKSAHIKTKKSPKLKRHL
                     RASAIVRKADEGRIQRMLGL"
     gene            1389..1736
                     /gene="rplT"
                     /locus_tag="PLO35_01715"
     CDS             1389..1736
                     /gene="rplT"
                     /locus_tag="PLO35_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOH78408.1"
                     /translation="MPRSANNVAAHRRRKKYMLAARGFFGRRGTIYRAARQTVERGMA
                     FSFAHRKQKKREFRKLWIIRINAACRLNDMSYSKFINGLHKANIEINRKTLAHLAWHD
                     SEAFAKLVEIAKG"
     gene            1899..2930
                     /gene="pheS"
                     /locus_tag="PLO35_01720"
     CDS             1899..2930
                     /gene="pheS"
                     /locus_tag="PLO35_01720"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOH78409.1"
                     /translation="MRSQLNELVEQAGESIAACRTPNDILNARAAYLGKKSMLNGLFA
                     RLRELPAEERPAFGQLINEARTSLENLLAQREQEIKSAAWEAKSGAALDLSMPGAVWQ
                     RGSFHPVTLVRRQIEEVFLGMGFEIAEGPEIEDEFHNFDALNTPADHPSRNLADTFYL
                     QNGKLLRTQTSTVQIRVMESFPPPIRIISPGRCYRNDKPDPSHSPVFQQVEALVVDRG
                     VSMSDLRDTLQNFARAMFGPEVKSRIRPHFFPFTEPSAEMDISCVNCGGQGCRVCKNS
                     GWLEMGGAGMVDPAVFKMVGIDPELYTGFAFGLGIERIAMLKYSIPDMRILFENDVRM
                     LRQFRGESL"
     gene            2927..5443
                     /gene="pheT"
                     /locus_tag="PLO35_01725"
     CDS             2927..5443
                     /gene="pheT"
                     /locus_tag="PLO35_01725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOH78410.1"
                     /translation="MIISRRWLAKYVQLDIDDAALNKALTFSGIEVEAVKHLPALPPQ
                     VISARVVHAEALAGSDHLKVCKVDTGDGSEPRQVVCGAANCHSGMLAVLAMPGAVLGD
                     LTIKSARLRGAESHGMLCSERELGISDDHSGIIQLPEGTPLGVSVNDLYELPDTIFEL
                     EITPNRPDLLGYFGIARDLSASLGLPLSFPELVTTPGRQDGSMALKLEIRDPELCPRY
                     TARLFDHIQIGESPLWIKSALIKTGLRPINNVVDITNYVMLETGHPLHAFDYDVLAGE
                     HENDACPAIIVRRAKEKETFFALDGKDHCLETRDLVIADGQKPSALAGVMGGKLSAIG
                     DQTSRVVLEAAAFHPGTIRKTSYEHKISTDSSYRFERHLSPVMAPHVSARAAELFVQV
                     AGGEAVGDLIDAYPAPLKPWMLGLRPCRFELLIGYKMEEKRIIDFLAKLGLKFMQYGN
                     WQDAAVTDPSQITCYHAEQEKAGVTEFDMSIECDHALWFAIPPWRVDLTREADLLEEL
                     ARLDGYDKVPVKTLPSMIMDRHAYKIRNKVAEYLIGLGFFETLSYSFNDPQQIKMLGI
                     DPEENTQKLLNPQSSNQALMRVSLLPQLMEHVRYNLNHGERDLKLMELARTYPAGMDH
                     EPLRCTALLTGNLRAAQWQNPPRQTSFYDVKAVVEGLFEMLNLSADRISACPRPWLMK
                     SHNLGWFLEDQAVAMIGSVDPLVADRFGIDLSLLGQELWFIDIDLEKVIELTRNQTVI
                     YKELPRFPGVSRDLSFLIPSQVSWADISRLVRSVDVELITSVEVFDEYRGKQIPEGFR
                     SLSIRLTMRDKEKTLTDERIDALVDSVVKLLIQECQIQMR"
     gene            5446..5766
                     /locus_tag="PLO35_01730"
     CDS             5446..5766
                     /locus_tag="PLO35_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78411.1"
                     /translation="MEIQAISLQEKIQKLIDQYTEAKNRLSVMEQQVNELTEENVQLI
                     GQIDSGNAGQALMQEKNRELEEKVKSLEQKCAELQAAMDGFQDIATDAISKIDSIFPD
                     LDEL"
     gene            5770..6039
                     /gene="zapA"
                     /locus_tag="PLO35_01735"
     CDS             5770..6039
                     /gene="zapA"
                     /locus_tag="PLO35_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOH78412.1"
                     /translation="MQSVEVEIFGRKFRLRTDDVEKTQTIVAEINNQLSDLQILYESL
                     DFTKLLLLLALQQQEQVLELQSQNIQLSSDLERLNLMIGKIIGEN"
     gene            6271..7842
                     /gene="rny"
                     /locus_tag="PLO35_01740"
     CDS             6271..7842
                     /gene="rny"
                     /locus_tag="PLO35_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOH78413.1"
                     /translation="MFTQEVIIALTLGALLGFGVALIIYLIRRGTTFRMIAHSQEIAE
                     EIKRNAKTEAETAKKAAILEGRDEWFKQKRGMEDEIRERQKELRIQEKKYNERLGSLD
                     KRLDSLDKKESNLSDQERKLKQKEEELLKRNSDVDRLIAEQRDRLAEIAGLSRDEAVA
                     RMREELISQARQVAANDAKLTIDQIKLDTSKKVAEILATAIQRMAVDHVSETTVSVVS
                     LPSDEMKGRIIGREGRNIRTFEKASGVDLIIDDTPEAVVLSCFDPVRREIARLSLEKL
                     IADGRIHPGRIEEVISKTGKEMEETLMKIGEKAVLETNIHNISPNLIKILGRLHYRTS
                     YGQNVLQHSIEAAWICGILAAELNLDQELARRAGLLHDIGKAVDHEFDGTHAIIGANL
                     ARKNGENKLIVNAIEAHHEEVEAISVYAGLTQASDAISGARPGARREMLETYMQRLAK
                     LEQIANAVDGVNKSYAIQAGRELRIIVEPAMVEDAQTSLLATEIAAQIEREVQYPGQI
                     KVTVIRETRQVAMAK"
     gene            7842..8645
                     /locus_tag="PLO35_01745"
     CDS             7842..8645
                     /locus_tag="PLO35_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOH78414.1"
                     /translation="MRLLFFGDVFGKAGRVVLAEQLEALKAEFQPDFIVINGENLADG
                     KGLTEKTLKPLLRAGVDAVTGGNHLWDRAESLDFIRSTPLIVKAMNYPVKTPGSMSYT
                     IVKQDLRLTVISLSGQIFMPPCDSPFAAFDKWHDEHSLPGACVLLDFHAESTAETRAL
                     GWHVDGRISALLGTHTHIQTADEEILPSGTAYITDVGMTGPHDSVIGMRKNIILEKLR
                     SAIPIRYEASDRGNQINAVCVDIDPDSGKALSIQRIRRWVEINHAREQE"
     gene            8647..9567
                     /locus_tag="PLO35_01750"
     CDS             8647..9567
                     /locus_tag="PLO35_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOH78415.1"
                     /translation="MEKVLSGKPVAERIKAFIKEQVQTLRLTPRMLLVQLEGDSASDY
                     YVQNIIRNGARLGIAVESRKLPTETDQTELLNLIASANSDPDLHGIMIQKPLPSHIDE
                     ALIGSAIDPDKDLDSLNPVNLGRIMMDMNGLTPATPTAVYALLRFYGIPTRGKHVVII
                     GRSAVVGKPLANMLLWKKPFADASVSVVHSRSAQLQELSRQADILVAALGKAGFVNAG
                     MIKENSVLIDVGINEVPGSDGKPVYVGDIDYNSCYDKAIAITPVPGGIGTVTSALLFV
                     NLLRACMGLKKDNKSIDDFLGLIFNENHND"
     gene            9594..11981
                     /locus_tag="PLO35_01755"
     CDS             9594..11981
                     /locus_tag="PLO35_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78416.1"
                     /translation="MQSKRIKVLTLSLLVIAVVLGITGCGKSGTMKANVAPSISITSY
                     EGFDDSDLLSPYANSLFLFQQKIYWNATDPDGVIAGFAYRVKDQNGNPIATPGNHFID
                     ATGAITPQNVLTKYGPGWVMHYLPNAPQDLPLDDPQARRTIWTSQKYAVINLPASDAN
                     GNPITLLSTFEVIAVDNRGEVTQTLAWRSFNATSARPQCFISTTKGNPNGSEVGSGIR
                     LSFSMKDTDPFIPEIPFKYEFKMMKVHPTTGATIAGTETAWINSVANSDPDIGHFLLT
                     RYTQPALTYDIENNVTNSKTKVIARVYDMAGVVSTVSDSTAITFAVKAGFRPKTLVYP
                     QKAYALGDNHFIDYTDESTPEILPFTIVGGAQRFATPFFRDFTNNRVAINSSNMKVWI
                     RWGWRGEYGQVPATGPINYTDNPYDKKVDTVLDRTTDLNYFSEITHFDLRYDGDEYEY
                     APYENDPSRHITDTDGKKWLRIPLNSPMGQTVVLTYPRISDGTHTFEVRCVDLQGEVD
                     PIPAEFTFTLEPLIPKANRQGILVIDDDSDNASESPEAIVDGKYANMISDYSGNKVFK
                     KRGAAGVTFQDQRMRHLSPTELQKYELVIYHSDRPTESGNLKVENDGLCLYMAKGGNV
                     VVSHAAKLAEVLQAFALANQKSFLSYFGLPFVSNPASFMSSSFITRPIFQSAVGQNLG
                     TGDVYPNMALQFGTENPSFNNLVNIRHGLSTIAYFGDAIGSTPGNSVTYRLGAKPTTY
                     PNFPPSQADYDLYNNKPVGIRRVNTNNRTWMFGFPLSYMVDADSKAMMNKILSEI"
     gene            complement(12187..13332)
                     /locus_tag="PLO35_01760"
     CDS             complement(12187..13332)
                     /locus_tag="PLO35_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOH78417.1"
                     /translation="MSWLIELRKKLHQIPEIAFEEHHSSALLREQLNILVKNHLSPEA
                     IRAGSVAPLVHGFENSSGFLVEYTCDDGPYRMFRADMDALPVSEDTACDFKSQRPGMM
                     HACGHDIHMSVLMGLIDRVLREQPRRNLLFLFQPAEEGKGGAESVLAEGILQQFHIEA
                     VFALHVASGLPVGTVSSRPGIFFGIPQEFDVRFLGKSAHVAFPEQGIDALAPATDFIQ
                     MMREDIAELSRRHRVIFHIGKIEGGSIRNVIADQCILEGTHRSLVKEARDSMNRLIES
                     NAHKAAVAYGAGYESDFLCSYDPVVNDAGLVSKLQSACGRLGYRFEEAAVAMTGEDFG
                     FFTSIYPGLLFWLGSGCDQPLHSPRFLPAEECIEVGVNVMWALAEAE"
     gene            complement(13329..14309)
                     /locus_tag="PLO35_01765"
     CDS             complement(13329..14309)
                     /locus_tag="PLO35_01765"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOH78418.1"
                     /translation="MKIAIVGATGEVGRMMIRNLAEFNVPISELDLYASRRSAGATLY
                     YVDHAIKVLELTESSLLKHYDYVLFSAGAGVARSYAPLGLNSCDAIIDNSSAFRKEKH
                     IPLVVPEINGDLLEGYEGIVANPNCSTIQMVLPLDVLNKLFRLRKVVVATYQSVSGSG
                     HKGVQTLQEQRDGSSSPGEYSSVIDLNVIPQIGALQDSGYCSEEEKMHCETRKILRNS
                     EIAISATTVRVPVIYGHSLAVYAEFEREVDLAVAEDALSKAPGISFAANTYTTPLDLG
                     NSNESHVSRLRYGTDLHSLNFWNVANNVRLGAATNAVKILMKHAVFSGKV"
     gene            14425..14500
                     /locus_tag="PLO35_01770"
     tRNA            14425..14500
                     /locus_tag="PLO35_01770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14458..14460,aa:Arg,seq:cct)
     gene            14517..16451
                     /locus_tag="PLO35_01775"
     CDS             14517..16451
                     /locus_tag="PLO35_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOH78419.1"
                     /translation="MNTIQPGCMALFYQAGLLVTGLVTGIEGTLLQIVSTDGASHALP
                     AARLCLRGTEVYDSSDPQRTLGDFQEQVHQNRDVLESALKVLSDGEGRNLDAITQELG
                     VLSDPRRFALFINLNQATHLVSHKKGLYRLLSATERQDAELSEKQRRERQDYLDQVQA
                     YIRDLAPNDSGQPLSPDLKRQLIAELQLLQREQSPPDLASLIRKTMPERDFDRALIEI
                     RLALGDLSPDTDPALSISGLPVNFAANVPGYQAPGITQPRGENPEVICVDDPDSRDYD
                     DAFSVVKTATGYRIGVHITDVAASLVPGSDLFEEVLERVSSLYLPAETVNMLPDCLAE
                     DLLSLRAGELRAVLSFYCDVDQDSGLRDWTFLRETIRVGSNISYAELDARLSRGEYQM
                     VKAFCDALRSERGATSQTSGNDLLYNLRVSGSRVRFKRIDFSSPARVMIEELMVLYNR
                     LMAAMASESDIPFLYRNISSYEDNVPNDDEDAPDQAVIPSQTGSQAYISTRPMFHPGI
                     GAKAYLHASSPIRRVVDLINQYQFHAWLEGRPASFSQEALDTLVPAIGKRLLLQREVV
                     RQSERYWLLRYLKQDWLHRPLDAVFIKTSRRGCVFELLPWGKRIIVDCDITPPVSYEL
                     KLLITTVDLTGQTCRAEVIL"
     gene            complement(16718..18346)
                     /locus_tag="PLO35_01780"
     CDS             complement(16718..18346)
                     /locus_tag="PLO35_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78420.1"
                     /translation="MKKLILLCAITLCVTIAHAGAWTWASSLISTGMVRAWDLGGSSL
                     NPAIWVGGQYDGSMSFNGIDYPSAGMSDAFVAKYSREGTPLWFRQFGSPDEDVCLSIE
                     AGTGGTDNCYLTGYFNDVMSVGSTVLTSAGMWDVFLGKLSPGGDVLWLKRFGGTLNDI
                     GYGITVDHEDNVIVAGWFADTIDFGGGISISSYGGSDMFVAKFDPQGTCLWAKHAGDE
                     GVDYGYKVDCSETGYIYVTGVAGQGANFDGISQSQDGLFVAKYSYDGSIMWVSSALGA
                     GPINIGIGKQDLLQYRGMVTGRVTGTAVFGDHVISSVDGSDDIFLAEFSPGNGTWINV
                     QRWGGTGSDKGRAVDVHDYMGSLTVASYENSVDFGSSILNSNGVWDCALLKGGPQAAA
                     VGFGSINTDVVSDVKRALMNKAITCGWYNGSMRLGNILLNSGSDAIQSGFIGMYDFDA
                     VATDDPILPPPVGLSCYPNPFSSNLEISSKDKTISKLQVFNQRGQLVRDLEHSSPNTW
                     RWDGQDSQGNLCATGIYLIRSAELRSFQKVLLLR"
     gene            18593..19834
                     /gene="rocD"
                     /locus_tag="PLO35_01785"
     CDS             18593..19834
                     /gene="rocD"
                     /locus_tag="PLO35_01785"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOH78421.1"
                     /translation="MSDTLKYGKVSSKESMELEEKYGAHNYHPLPVVLAKGEGVYLWD
                     PEGNRYYDFLSAYSAVNQGHCHPRIINALIEQAKELTLTSRAFFNNKLGEYEKYITDF
                     FGYDMVLPMNSGAEGVETALKLARKWAYTKKGVEKDSAIVVFCENNFHGRTLAVVSAS
                     TDPDCYEGFGPFLPGIAKVAYNDVNALREYLNQHGKNVAAFIVEPIQGEAGVMVPDDG
                     YLKACYDLCKEHNVLFIADEIQTGIARTGKLLAVDYENVRPDILILGKALAGGVLPVS
                     AVLADREIMLTIKPGQHGSTFGGFPLACAVAKAALEVVKEEKLAERAYEMGEYFRALL
                     RAIDHPMLKLVRGKGLLNAIVVEPRDGIEAWDVCLMLKEKGILCKPTHRHIIRLAPPL
                     VITKEQLKDAADIIAGVFASL"
     gene            19963..20844
                     /gene="era"
                     /locus_tag="PLO35_01790"
     CDS             19963..20844
                     /gene="era"
                     /locus_tag="PLO35_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOH78422.1"
                     /translation="MDSSFRSGFVAIIGRPNTGKSTFLNRVLGEKLSITSPKPQTTRY
                     AIKGILNRPDLQIVFIDTPGFLKPRYEMQTRMLKIWSDAFKNVDLVLFITDLKDFPTE
                     YDHEVLEILNKSRAHQIAVFNKLDLRPEIDREALKALLPASVDEAHFISSVTGENLDA
                     LMQSIINFVPYHDPYYEADQLSDLPTRFFATEVIREGIFHIFEQEIPYSTAVVIEKYT
                     ELPDEVVVEAQIWLERQSQKLIVVGRKGDGLRKIREYAEAQLSAFLDQPVRVHLWVKI
                     VPNWRKKNSSLRELGFG"
     gene            20943..21809
                     /locus_tag="PLO35_01795"
     CDS             20943..21809
                     /locus_tag="PLO35_01795"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOH78423.1"
                     /translation="MRHIKVPGAGREQGADEAMASGLDTLLVVDIGNTNIVCGLFVAR
                     ELIRTARFHSSVNRTADEYYSLLIPFITETGDINITDVAIASVVPELTRMWQHLFQRH
                     FELSAKIITALSPLGLQYRVADPGFIGADLVVNAFAAWQKYHSSTIIIDLGTATTVQF
                     VTDKGSFEGTAIIPGMKTGARNLFDKAALLSEIEISAPPALIGTTTSAALLSGIVNSH
                     AFLLETYIHKLKQQYFDHKRILTILTGGMADLLKPLMPSVDCVDKTLTLEGLMLAAEK
                     LAATVPTEQLRT"
     gene            21813..28019
                     /gene="sprA"
                     /locus_tag="PLO35_01800"
     CDS             21813..28019
                     /gene="sprA"
                     /locus_tag="PLO35_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOH78424.1"
                     /translation="MIKRTLAIAVSIGILCILAANVMPLYLHLPGYHPLIDNQVDVYK
                     LDKGIGFTKSRQYLIPSIAPVKTETVDLPNQRVIISTSIDELKITDDRVVSFDSYFRS
                     LQRKAFRKSLFAQVKTKRQQTVVTTSGLIGEFKLELPAIAIPKAVQKVLGSSAGRLNL
                     DGTQKITIEAGSTKRKLQPIYETQSSSRFDLKMEQETNLRLSGKIGDKIAVNLKYNSK
                     QDEQLFDPNNVSIKYTGTEDEVIQSIEAGNITLALSGSRYISYSTSSQGLFGITSKLK
                     YGNMDLTLIASKEESQKNTQSYIGTSQADSTIFDSKQYVARTMYYLDRPDSLYQLYQE
                     SDPDCKTGYAGNAIRTGANGAWMISNADRLPANGTVRVFIDDLVATNNVGSALGDTIQ
                     ISNQDYQLNWDELIEGADFVTDYDSGIITINRNVLTSHVVGVRYTTRGGEGITPVSVP
                     APSAEELANPDLLYTKIIRNYNQQYSPDDPNSVWDYQMRNIYKMSSTNIRNDGFSLDI
                     YTENVDRTRNYFVADSLQVGQNVSYSDYLRMDSNGDSKVDGSDTTVNLVSGLIIIPML
                     RPFEPLGDEIIYQMENDSYSINPLDTELFIAAKGKIGRDAVDLQQGNILKGSVRVKVN
                     GNELKENLDYIVDYDFGRITFLTPAGKDPDAKIEIDYEFRGMFDVASKSLAGVRMDWN
                     LTDYAKLGGTFIYRSENVADKRPRIGNENIEMMLANLDGNITLKPKFITRWMDWLPFI
                     STTAESRLTLSGEIAYTLPNIYGDPDGKKNSIYLDDMESIMDSYPLPVTISSWVRGSK
                     PWGTTLAKGIPNWYNPKNVKRSELVDDPETLTDREKNEFVTVLAVKNTPANIGVGGSN
                     VQSWAGVMKYLGNQLDFSQKKYIEVLVKLDVKQGETVPDPVIKIDLGDLNEDFYTEYG
                     GLGVLNNEDLNNDGVLTLDEDIGLDMKDRVTSGSDPNDISPSTIQNHHYEAYPGINGT
                     EGNRALDTEDLDGNGILNQVDRYFSYSFAITGTQHLVNINHSNWRLYRIPLHDPNVYT
                     IVNNSAAGVLPSMQRISFARITVETDIPARIYIADMAIVGNKWQDFGVRDFNNTQFQE
                     DVVTSLGTTYLSGIVNNQTNSRYRPPEGTVYIEDRRQSVESSLTLDVKKLQPGNQVLL
                     RQRSTEAYSLLSYGKLKLYVYPEALRTGEEHPDKVDFVLRLGADEFTYYEIRQRVSIY
                     DWETNMDKDRWLLYTYQLSDITKLREANPDTTQGFIQVDENTYFSFRGRPTLSTIRDV
                     CLGVSNPSGSSIYNGMFYFNELLLTDPYEDVGVAQRVTLNAALADFSTLDVDYEQKSE
                     NFNPNIQRGRANTFTSSTSLNITNKYFLGKLFPRTWDVDIPITLSRNYTLGIPRYRAN
                     SDLLRESIDADSTKLRERNETLLYAADFAFSVKSPPKNKILLYTLYRSSLSGRIESSV
                     RNQPTTRDSTFTLRGTYNYNIGFPSEKVSFKLFNNYRLGFFPTTWNNSLTYNSTTPRS
                     ENWERRQIDGDYITYWFPRQGVLPTELLTTDSNLNWALTSDIGLTARLNTKRDLIQKQ
                     LWEKINVGKLTEYVQDLGLNYNPNFLRSVMNLTASGSTRYSELQRRYYDNSSGQQVEV
                     YQSDGGVNRTFRANLTLMNSTLLGRWAQNLTQKHQAKADAKADADKTTDKDPADKPED
                     KPQDTDEETKRKKEQEMKDEEAQKRAEEEKRKAEEAAFWDNQPKDDPFGGTGDGGFPD
                     FKDGEFGFGDPKEGGMGPGASYQGSDPGSPYVPPKDVKYLPALVLGYLSKLKNITVSY
                     QNGYTMNYLRKEERPPFAFQIGLPHTVDPGFLDATGNDNTFTLSSGIMISRNIDSVIN
                     YSYTINKRYSSASNQNTAITFPDITLSLMDFEKWVGLSKYLSSTRINTGFQRSIRSSG
                     NIDWVKPKQETNGIAFNPLIGFASTLFKKVSTNLSFSFAQSTNTTDMESYDVIKKTNT
                     QSLNGNLSYSFRGGRGFTIPFTKKKIHIKNELTSSLAFVYERNFDDTTGREGNYQVDR
                     DTTRLAFTPGATYQFNQDIRGGLTGTWENTSDKRRDDGVRIFRIGVWVEVNL"
BASE COUNT         7457 a         7817 c         7422 g         6370 t
ORIGIN      
        1 tatctcgcgg gtgtactggt tgaagtccaa tcggcccgca aactcgctgc cacgcataca
       61 gttatcgtgg ccgcttgaac tccaacgggg cttatgttag tctcttgttt agtcagtttt
      121 tgcacaagct ctgaagccag tgttacggct atgtgcaacg gtctatcgtt gatagcagga
      181 atcattgaaa actgaggtat atgtcccggg ggaagaatac tggttgacag aaacagcgcc
      241 ctgagatttg tggaactaca aacgaagctt gtctgcttct gccttaccgc aaaggcgcgc
      301 gtaaggccat gaaataaaag cgaatatcat agatatttat cgtgaagcag gcgaagccga
      361 tgaggtttcg cctttttcat ttccggtaag aaggaggttc ttataatccg gataaagcgt
      421 ggaaaggcac gtaccaaaga ggtagtgccg aaggaaagga taaacggcca gatcacgtct
      481 tccaatgtac gtctgatcgg agctgacgga cgtcaggtgg gtgtcgtgtc catccgtgaa
      541 gcattacgca gagccgaaga gcaggatctg gacctggtgg agatttcccc caacgtggat
      601 cctcccgtgt gccgtataac ggacttcagc aagtattatt tccagaaaga gaaaaaagcc
      661 aaggaagccc gtaaaaaaca gcatgaagtg gaagtgaagg agatcaaata cggtcccaac
      721 actgaagatc atgactacgg cttcaaaaag aacaatgcca tcaagtttct caagcagcac
      781 aacaaggtca agttcacggt gcgcttcagg ggacgtcaga tggctcacaa ggaattggga
      841 ttcaacctgt tggaacgcat caaagccgat cttgcgcaca tcgcggacgt cgatgccgag
      901 cccgtgtcag accgcaatct gctgtttctg gtgatgagcc ccaaaaagga aatagacaag
      961 atcctgggcg gggagaagaa agccactcca gtggccgaag aagccgttcc agccgttcct
     1021 gagaccgtag aacccaagcc cgaaagctaa aaccaaccct gccaggctgc agtggcgatt
     1081 ccagaaagcc agacaccgcg gacgaagatg tttgcggatt cattctgatc gccaagccca
     1141 gccaaggctt gcataaattg atataaaata aggagataaa gatgccaaag atcaagacaa
     1201 accgttccgc ggccaagcgg ttcaaggtta ccggttccgg caagattgtg cgtcaccatg
     1261 ccaagagcgc gcatatcaaa accaagaaat cacccaagct gaaacgccat ctgcgcgcca
     1321 gtgccatcgt ccgcaaggct gacgagggaa gaattcagcg gatgctggga ctataaagga
     1381 gatataccat gccaagatca gccaataatg tagctgccca tcgcagaaga aaaaaatata
     1441 tgctggccgc ccgcggcttt tttggtcgtc gcggtaccat ctatcgtgcc gcccgtcaaa
     1501 ccgtggaacg cggtatggcc ttttcctttg ctcatcgcaa acagaagaaa agagaatttc
     1561 gcaaactctg gatcatccgc atcaacgcgg cctgccgctt gaacgatatg tcctacagca
     1621 agtttatcaa tggccttcac aaagccaata tcgagatcaa ccgcaagacc ctggcccacc
     1681 ttgcctggca cgatagcgaa gcttttgcca agctggtgga aatcgccaag ggctgaatga
     1741 ctggcagcgt cgatttgcac atcggcgcgt tctggaaatt tataaactga tccaagccat
     1801 gcgtatgatt ccggagactt ggattccgct ccggccaacg cgtatcacct accgcaaacg
     1861 ctgacgtttg cggctccatc aatacataag gataagcggt gcgatcacag ctgaatgaac
     1921 tggtagagca ggcaggcgag tccatcgccg cctgccggac tcccaatgat atcctcaatg
     1981 cccgggctgc ctatctgggc aaaaagagca tgctaaatgg tctttttgcc cggctgagag
     2041 aactgccagc cgaagagcgg cccgcctttg ggcaattgat caatgaggcc aggaccagcc
     2101 tggaaaacct gctcgctcag cgtgaacagg agatcaagag tgccgcctgg gaagctaaga
     2161 gcggcgcggc cctggacctc agcatgcctg gcgcggtctg gcagagagga tcatttcatc
     2221 ctgtcaccct ggtacggcgg caaatagaag aggtgtttct gggcatgggt ttcgagattg
     2281 ccgaagggcc agagatcgaa gatgaatttc acaacttcga cgccctcaac actcccgcgg
     2341 accatccatc ccgcaacctg gcagacacct tctatctgca gaatggtaaa ttgctgcgga
     2401 ctcagacctc cacggtgcag atcagggtga tggaaagctt tccacctcct atccggatca
     2461 tttctcccgg acgctgttat cgcaacgaca agcctgatcc ctcccattct cctgtctttc
     2521 aacaagttga agctctggtg gtggataggg gagtgtctat gtccgatctg agggacaccc
     2581 tccaaaactt tgcccgggcg atgtttggtc ccgaggtcaa atcccgcatc cggcctcatt
     2641 tctttccctt tacggagccc agcgctgaga tggatatcag ctgtgtgaat tgtggcggac
     2701 aaggctgccg ggtatgcaaa aactctggct ggctggaaat gggtggagcc ggaatggtgg
     2761 accccgccgt gttcaaaatg gtgggtatcg atccggaact ctacaccggt tttgcctttg
     2821 ggctggggat tgaacgcatc gccatgctca aatacagcat ccccgatatg cgcatcctgt
     2881 ttgagaacga tgtgagaatg ctgcgccagt tcagaggaga gtcactatga tcatatccag
     2941 acgctggctg gccaagtatg tccagttaga tatcgatgac gcggctttaa acaaagccct
     3001 cactttctcc gggattgaag tggaagccgt taaacacctt ccggccctgc ctccccaagt
     3061 gatcagtgcc cgcgtggtcc atgccgaagc tctggctggg agtgaccatc tcaaggtgtg
     3121 caaagtcgat acgggagatg gatctgaacc tcgtcaggtg gtgtgtggag cagccaattg
     3181 ccacagtggc atgttggccg tgctggccat gccgggggcc gtattgggtg atctgacgat
     3241 caaaagtgcc agactgcggg gagcggaatc ccatgggatg ctctgctccg aacgggaact
     3301 ggggatatct gatgaccact ccggcatcat ccaattgccg gaaggcaccc cgcttggtgt
     3361 gagcgtcaat gatctctatg aactgcctga taccatcttt gaactggaga tcacacccaa
     3421 ccggcctgat ctgctgggtt actttggcat tgccagagac ctcagtgctt cactgggact
     3481 gccactcagc tttccggaac ttgtcactac ccccggacgc caggatggct caatggccct
     3541 caaactggag atcagggacc cggagctctg tcctcgttac acagccaggc tgtttgatca
     3601 tatccagatt ggcgaatccc ctctctggat caagagcgcg ctgattaaaa ccggcctcag
     3661 gcctatcaac aatgtggtgg atatcaccaa ctatgtgatg ctggaaaccg gtcatccctt
     3721 gcacgcgttt gattatgacg tgctggcagg tgaacacgaa aacgatgcct gcccggccat
     3781 cattgtcaga cgcgccaaag agaaagagac tttttttgca ttggacggca aagatcactg
     3841 cttggagacc cgcgacctgg taatcgccga cggccagaaa ccttccgccc tggcgggtgt
     3901 gatgggtggc aagctctccg cgatcggaga tcagacctcc cgcgttgtat tggaggccgc
     3961 agcctttcat cccgggacga tcagaaagac ctcctacgag cataagatca gcaccgattc
     4021 ttcctatcgt tttgaaaggc atctcagccc cgtcatggct ccccacgtga gtgccagggc
     4081 agctgagctg tttgtccagg tggctggtgg agaagccgtt ggcgatctga tcgatgccta
     4141 ccccgctcca ctcaaaccat ggatgcttgg cctcagaccc tgccgcttcg aactgctgat
     4201 cggttataag atggaagaga agcggatcat cgactttctt gccaaactgg ggctgaaatt
     4261 catgcaatat ggcaattggc aggacgcggc agttactgat cccagccaga taacctgtta
     4321 tcacgctgaa caggaaaaag ccggagtcac agagtttgat atgtccatcg agtgtgatca
     4381 cgccctctgg tttgccattc ctccctggcg agtggacctc acccgggagg cagacctcct
     4441 ggaagagctg gcccgtctgg atggctatga caaagtgcca gtcaagacat tgccatcgat
     4501 gatcatggac cgccatgcct acaagatcag gaacaaggtg gcggaatatc tcatcgggct
     4561 gggctttttc gagacgctca gctacagttt caacgatcct cagcagataa agatgctggg
     4621 catcgatccg gaagagaata ctcaaaaact cctgaatccc cagagcagta atcaggccct
     4681 gatgcgcgtc tctctgttgc cacaacttat ggaacacgtg cgttataatc tcaatcatgg
     4741 cgagcgtgat ctcaagctga tggaactggc caggacctac ccggccggta tggaccacga
     4801 acctctcaga tgcacagctc ttctgaccgg gaatctacgg gccgcgcagt ggcaaaaccc
     4861 tccccgtcag acaagctttt atgatgtcaa agcggtggtg gaagggctgt ttgagatgct
     4921 taacctttca gcggacagaa tttccgcatg tcccagaccg tggttgatga agtctcacaa
     4981 tctgggctgg tttttggaag atcaggctgt agccatgatc gggtctgtgg atcctttggt
     5041 ggcagataga tttgggattg atctctcact gctgggccag gaactgtggt ttatcgatat
     5101 cgatttggag aaggtgatcg agctgaccag aaatcagacg gtgatctaca aggaacttcc
     5161 tcgctttccg ggtgtgagca gagatctttc tttcctgatc ccaagtcaag tcagttgggc
     5221 agatatctcc aggctggtgc gatcggtgga tgtggaactg attacatccg tggaagtatt
     5281 tgacgaatac cgcggaaagc agatacccga aggattcaga agcctttcta tcaggctcac
     5341 catgcgggac aaagaaaaaa cattgacaga tgagcgcatt gacgcgctcg ttgattcggt
     5401 agtgaaacta ctcattcaag aatgccagat acaaatgagg taatcatgga aatacaagcc
     5461 attagcttgc aggaaaagat acaaaagctg atcgaccagt acacggaagc caaaaacagg
     5521 ctaagcgtga tggaacagca ggtgaacgaa ctcacggaag agaacgtaca gcttatcggt
     5581 caaatcgaca gtggtaacgc cggacaggca ttgatgcagg aaaagaaccg cgagctggaa
     5641 gaaaaagtca aatccctgga gcagaagtgc gctgaactcc aggccgccat ggacggattc
     5701 caggacatag ctactgacgc catcagcaag atagacagca ttttcccgga cctggacgag
     5761 ctctaagcaa tgcaatccgt ggaagtggag atctttggcc gcaagtttag attgcgcacc
     5821 gatgacgtgg aaaagaccca gacaatcgta gccgagatca acaaccagct atctgatctg
     5881 caaatcctct acgaaagcct tgattttacc aagctgctgc tccttctggc cttgcagcag
     5941 caggaacagg tgctggagct gcaaagccag aatatccaat tgagctccga tctggagcgt
     6001 ctaaacctga tgattggaaa aatcatcggg gagaattaat cttagcctgc ggaacccgtg
     6061 atatggattg cataaaagct ataaaaatca tgggaaccga gccatgggtg gcatgcgagc
     6121 cggctggttc cggatacata aagcccacaa ggtaggtgcc cacctcatgc ttggcttttg
     6181 acagacaagc atacacggtt cacgcgggcc tttttttgaa ccaggcatgg tccctttcgg
     6241 tccatcctca atagatacaa ggataaatga atgtttactc aagaagttat catagcgctg
     6301 acgctggggg ctctgttggg ctttggcgtg gcgctgatta tttatctgat ccgtcgcggc
     6361 accactttcc ggatgatcgc tcattcccag gaaatcgcgg aagagatcaa acgtaacgcc
     6421 aagacggaag ctgagaccgc caaaaaagct gccattctgg aaggcaggga tgagtggttc
     6481 aagcaaaaac gcggcatgga agacgaaatc cgcgaacgcc agaaagagct tcgaatccag
     6541 gaaaagaaat acaatgaacg gctcggcagt ctggacaagc gcctggattc cctggataaa
     6601 aaagaaagca acctcagcga tcaggaacgc aaacttaaac aaaaagaaga agagctcctg
     6661 aagcgcaact cggatgtgga cagattaatc gcggaacagc gtgatcgtct ggctgagatt
     6721 gccggcctta gcagagacga agctgtagcg cgtatgcgtg aagagctgat ctcccaggcc
     6781 agacaggtgg ccgccaacga tgccaaattg actatcgatc agatcaaact ggataccagt
     6841 aaaaaagtgg ctgaaattct ggctaccgcg atccagagaa tggccgtcga tcacgtctcc
     6901 gagaccactg tctctgtggt ttcactgcct tcagatgaga tgaagggacg catcataggt
     6961 cgcgaaggcc gcaacatccg cacctttgaa aaagcctccg gagtagattt gatcatagac
     7021 gacactcccg aagcggtcgt gctgtcctgc tttgatcccg tccggcgtga aatcgcccgc
     7081 ctctctttgg aaaaattgat cgcggacgga cgtatccacc ccggtcgcat cgaagaagtg
     7141 atctccaaga ccggcaaaga aatggaagag accctcatga agatagggga gaaggctgtg
     7201 cttgagacta acattcataa catctctccg aatctgatca agattctggg ccggctgcat
     7261 tatcgtacca gctatggtca gaacgtttta cagcattcca tcgaggctgc ctggatctgt
     7321 ggcatcctgg ctgcggaact caatctggac caggaactgg cccgtcgggc cggcttgctt
     7381 cacgatattg gtaaagcggt tgatcacgaa tttgacggta cccatgccat tatcggtgcc
     7441 aaccttgcca ggaaaaacgg tgagaacaag ctgatcgtga atgccatcga ggcccaccac
     7501 gaagaagtgg aagcgatttc cgtctatgct ggtctcacgc aggcttcgga tgccatttct
     7561 ggtgcccgtc ccggtgccag acgtgaaatg ctggagacct atatgcagcg tttggccaag
     7621 ctcgagcaga ttgccaacgc cgtcgatggt gtcaacaaat cctacgccat tcaagctggc
     7681 agagaacttc gcatcatcgt ggaacccgcc atggtggaag acgctcagac ctcgctgctg
     7741 gccaccgaaa ttgccgctca gatcgagcgt gaagtgcagt atccagggca aatcaaggtc
     7801 acggtgatcc gtgagacacg ccaggtggca atggccaagt gatgcggctg ctattctttg
     7861 gcgacgtgtt tggcaaagcc ggacgcgttg tcctggccga acaactggaa gctttgaaag
     7921 ccgagttcca gcctgatttt atcgtcatca acggcgaaaa cctggccgac ggcaaaggtt
     7981 tgacagaaaa gaccctcaaa ccgctcctca gagctggagt ggatgccgtc accggaggta
     8041 atcatctgtg ggatagagcg gaatctctgg attttatccg ctctacccct ttgatcgtaa
     8101 aagccatgaa ctatccggtt aagactccag gcagcatgag ttacacgatc gtcaagcagg
     8161 atttgaggct cacggtgatc agcctttcgg ggcaaatctt tatgccgccc tgcgattctc
     8221 cttttgccgc tttcgacaag tggcacgatg aacacagcct gccaggggct tgcgttttgc
     8281 tggattttca tgccgaatcg accgctgaaa ccagagcgct gggctggcat gtggatggca
     8341 ggatcagtgc cctgctgggt actcatactc atatccagac agccgatgag gagattcttc
     8401 cctctggaac ggcttacatc actgatgtgg gaatgactgg cccgcatgac agtgtaatcg
     8461 gcatgcgcaa aaacattatc ctcgaaaagc tgcggagcgc gatacccatc cgttatgaag
     8521 cttctgacag gggcaatcag atcaacgccg tctgcgtgga tatcgatcct gacagcggca
     8581 aagccttgag catccagaga atcagacgct gggtggaaat caatcacgcc agggagcagg
     8641 agtaaagtgg aaaaagtatt atcgggaaaa ccggtagccg aacggataaa agcctttatc
     8701 aaagaacagg tgcaaactct gagactgacg ccacgcatgc tcctggtgca gttggaaggg
     8761 gattccgcgt ccgattatta cgtgcaaaac atcattcgca acggtgcccg attgggcatt
     8821 gcggtagaat cacgcaaact gcccacggaa actgatcaga cggaactgct gaacctcatt
     8881 gcaagcgcca atagcgatcc ggacctgcat ggcatcatga ttcagaaacc gctgccctct
     8941 catatagacg aagccctaat cggcagcgct atcgatcccg ataaggacct cgacagcctt
     9001 aatccggtga atctgggtcg cattatgatg gatatgaacg gtctcacgcc cgcgactcca
     9061 acagccgttt acgcgcttct gcgcttctat ggcattccca cgcgcggcaa acatgtggtc
     9121 atcatcggtc gcagtgccgt ggttggtaaa cctttggcca atatgctgct ctggaaaaaa
     9181 cccttcgccg atgccagcgt gagcgtggtc cacagtcgca gcgctcaact gcaagaactg
     9241 agcaggcagg cagacatcct ggtggctgct ttgggcaaag cgggatttgt gaatgccgga
     9301 atgataaaag aaaactcggt gctgatcgat gtgggcataa acgaagttcc tggttccgat
     9361 ggcaagcctg tgtatgtggg tgatattgac tataactcct gctatgacaa agctatagct
     9421 atcacacccg taccgggagg aataggtacc gttacctccg ccttgctgtt tgtaaacctg
     9481 ttgagggcct gtatgggctt gaaaaaagac aataaaagta ttgacgattt tctaggattg
     9541 atttttaatg aaaatcataa tgattaatac cacctacttg tttgggagga acgatgcaaa
     9601 gtaaaaggat taaggtcctt acgctatcac tgttggtgat cgcggtcgtt ttgggaatca
     9661 ccggctgcgg taaatccggc acgatgaaag ccaatgtagc accctcgatc tccatcacgt
     9721 catatgaagg ttttgatgac agtgatttgc tgtcacccta tgccaattcc ttgtttttat
     9781 tccagcagaa gatctactgg aatgccaccg atcccgacgg tgtgatcgcg ggctttgcct
     9841 atcgggtcaa agaccaaaac ggcaatccca tcgccactcc gggaaatcac ttcatcgacg
     9901 ccaccggagc catcactccc cagaacgtgc tcaccaagta cggtcccggc tgggtgatgc
     9961 attatcttcc caacgctcct caggatttgc cactggacga tcctcaggcg cgtcgcacca
    10021 tctggacttc ccagaagtac gcggtgatca atctcccggc ttccgatgcc aacggtaatc
    10081 ccatcacgct gctcagcacc tttgaagtga tcgcggtgga taatcgtggt gaagtgactc
    10141 agactctggc ctggaggagc ttcaacgcca cctcagcccg cccgcagtgc ttcatttcaa
    10201 ccacgaaagg caatcccaat ggcagcgaag tcggctctgg catcaggctg agtttttcca
    10261 tgaaagacac cgatcctttc atccctgaaa ttccgttcaa atacgagttt aagatgatga
    10321 aggtgcatcc caccactggg gccactattg ccggtaccga gacagcctgg ataaattccg
    10381 tcgccaattc cgatcccgat atcgggcatt tcctcctgac ccgttatacc cagccggctt
    10441 tgacctatga tatcgaaaac aatgtgacca attccaagac caaagtcatt gccagagttt
    10501 acgatatggc cggggtggtt tccaccgtca gcgattccac tgccatcact tttgccgtga
    10561 aagctggttt ccgtcccaag accctggtct atccacagaa ggcctacgcg ctgggtgaca
    10621 atcactttat cgactacacc gatgaatcca ctccggagat cctgcccttc acgatcgtag
    10681 gtggcgcgca gcggtttgcc acacctttct tccgtgactt caccaataat cgcgtggcca
    10741 tcaacagcag caacatgaaa gtctggatca gatggggatg gcgaggtgag tatggtcagg
    10801 tgcctgctac cggtcctatc aactatacag ataatcccta cgacaaaaaa gtagacaccg
    10861 tactcgatcg caccactgat ctgaactatt tcagtgagat cacccacttc gacctccgtt
    10921 acgacggcga cgaatatgaa tacgctccct atgaaaacga tcccagccgc catattacag
    10981 acacagatgg caaaaaatgg ctgcgtattc cgcttaattc tcccatgggc cagaccgtgg
    11041 tgctcactta tcccaggatc agtgacggca cccacacctt tgaagtccgc tgcgtggacc
    11101 tgcaaggtga agtagatccg attccggccg agttcacctt cacgctggaa cccttgatcc
    11161 ccaaggccaa tcggcaagga attctggtga ttgacgatga ttccgataac gccagcgagt
    11221 ctcccgaggc gatcgtggat ggtaagtatg ccaatatgat ctcagattat agcgggaaca
    11281 aggtattcaa gaaacgcggc gcggctggag tcactttcca ggatcagcgc atgcgtcatc
    11341 tttcacccac agagctccaa aagtatgagt tggtaatcta tcacagtgac cgtcccaccg
    11401 aatcggggaa cctcaaggtg gaaaacgatg gactgtgcct ctacatggcc aagggaggaa
    11461 acgtggtggt ctcacacgct gccaagctgg cagaagtgtt gcaggccttc gctctggcta
    11521 atcagaaatc cttcctcagc tattttggcc tgccctttgt gagcaatccg gctagcttca
    11581 tgagctcgtc gtttatcacc cggccgatct tccagagtgc tgttggccag aatttgggga
    11641 ccggagacgt gtatcccaac atggccttgc agtttggcac cgagaatccc agcttcaaca
    11701 acctggtaaa tatcagacac ggtctctcga cgattgccta ttttggagac gcgatcggca
    11761 gcacccctgg caacagcgtt acctacagat tgggcgctaa acccaccacc tatccaaact
    11821 tcccgcccag tcaggctgat tatgacctct acaacaacaa gcctgtgggg attcgcagag
    11881 tgaataccaa caaccgcacc tggatgtttg gtttcccgct ctcctacatg gtggacgccg
    11941 attccaaagc catgatgaac aaaatcctca gcgagatcta aacccaaaca agttgatata
    12001 caaaagcggc cgtgtgtttg tcacgcggcc gcttccttta ttttatggct gagttatggc
    12061 tcagcgcatc gatttgatca attcatcaat tccccggtat cgggagccgg ggaaagcaca
    12121 ctcagggatt aacaggattc atttctggag ggcaggcagg tccgtcaaac ccaatgtgac
    12181 aggctgctat tcagcctcgg cgagggccca catcacgttc acgcccactt caatgcattc
    12241 ttcagctggt aaaaaacggg gtgaatgcag tggctgatca cagccggaac ccagccagaa
    12301 gagcaaaccc ggatagatgg aggtaaagaa cccaaaatct tctccggtca tggcgaccgc
    12361 tgcttcttca aaacgatagc ccaaacgacc gcaagcgctt tgcagcttcg acaccaggcc
    12421 ggcatcgttg actaccggat cgtagctgca gagaaaatcg ctctcatagc cagcgccata
    12481 agctacggcg gctttgtgag cgttgctctc gatgaggcgg ttcatgctgt ccctggcttc
    12541 ctttacaagg ctgcgatggg tgccttccag gatacactgg tccgcgatca cgttgcgaat
    12601 gctgccaccc tcgattttcc ctatgtgaaa gatcacccgg tgccgtcttg acagctcagc
    12661 aatatcctca cgcatcatct ggataaaatc ggtggcgggg gcgagggcgt cgatgccctg
    12721 ctccggaaag gctacatgcg cgcttttacc aaggaaacgt acgtcaaact cctgcgggat
    12781 gccaaaaaag atccctggcc ggctggaaac tgtgcctacc ggaagcccgc tggccacgtg
    12841 gagggcaaat actgcttcga tatgaaactg ctggagaata ccttccgcca acacggattc
    12901 ggcccctccc ttgccttctt cggcaggttg aaacagaaag agcagattgc gcctgggctg
    12961 ttctctgagc acccggtcga tcaaacccat caatacactc atgtggatat catgtccgca
    13021 ggcgtgcatc atgccaggcc tttgcgactt gaaatcgcag gctgtatcct ccgagaccgg
    13081 cagggcgtcc atgtccgctc taaacattct gtagggaccg tcgtcgcagg tgtattccac
    13141 caaaaagccg ctgctgtttt caaatccatg cactagcggc gccacgcttc cggctctgat
    13201 ggcctcaggg cttaagtggt tcttgaccaa aatgtttagc tgctcccgca gcagcgcgga
    13261 gctatgatgc tcttcgaaag cgatctcggg aatttggtgc agtttcttgc gcagctctat
    13321 cagccagctc atacttttcc actgaagacg gcgtgcttca tcaggatttt gaccgcgttg
    13381 gtagcggctc ccagacgcac gttattggcc acgttccaaa agttcaggct gtgaaggtcg
    13441 gtgccgtaac gcagacggga aacgtggctc tcgttggaat ttcccaaatc caggggagtg
    13501 gtgtaggtat tggccgcaaa actgataccc ggagctttgg agagggcatc ttccgccacg
    13561 gcgagatcta cttcccgttc aaactcggca tagacagcca ggctgtggcc atagatcacg
    13621 gggacgcgaa cggtggtggc agagatggca atctcactat tgcgcagaat cttgcgggtc
    13681 tcgcaatgca ttttttcctc ttccgagcaa tatccactgt cttgcagagc tccgatctga
    13741 gggatcacgt tcaaatcgat gacggaggaa tactctccag gtgaggatga tccgtcccgc
    13801 tgttcttgta gtgtctgcac acccttatga ccactgcccg agacgctttg ataggtggcc
    13861 accaccactt tgcgaagcct gaacagcttg ttcagaacgt ccagcggcag caccatctgg
    13921 atagtggagc agttaggatt ggcgacgatg ccctcatagc cttccagcag gtcaccgttg
    13981 atctcgggta ccaccagagg aatatgcttt tctttgcgga aggcggagga gttgtcaatg
    14041 atggcgtcac aggagttgag ccccaggggg gcatagctgc gggccactcc ggccccggca
    14101 gagaaaagca cataatcata atgtttgagc agggagcttt cggtaagttc cagcactttg
    14161 atggcatggt ccacgtaata cagagtcgcg ccggctgagc gtctggacgc gtagagatcg
    14221 agttcggaga tcggcacatt gaattccgcc agattgcgga tcatcattct gcccacttca
    14281 ccagtggcgc caacgatggc tatcttcatt tatgatatcc ttatagttag tattaactga
    14341 gcgtgccttt tctatcgctt gggggctttt gtcaatgcaa aacttgacaa aaaacggccc
    14401 cactcaaatt tagcgcaaga tcgtgcccca gtagctcagg ggatagagca gcggtttcct
    14461 aaaccgttgg tcggatgttc gaatcatccc tggggcgcca tctgataaag gatgatatga
    14521 atacaatcca gcccggctgc atggccctct tctatcaagc cggcttactt gtcaccggtc
    14581 tagtaacggg catcgagggt actttgctgc agatcgtcag cacagacgga gcttcccatg
    14641 cccttcctgc tgcaagacta tgtctcagag ggactgaggt ttacgatagc tctgacccgc
    14701 aaagaacgct tggtgatttc caagagcagg tccaccaaaa ccgtgatgtc ctggaaagcg
    14761 ctctgaaggt tttatccgat ggagaaggca gaaatctcga tgctatcact caagagcttg
    14821 gcgtcctgag cgatccgcgc cgttttgccc tgtttatcaa cctgaaccaa gccactcacc
    14881 tggtttctca caagaagggg ctctaccgtc tgctcagcgc tactgaacgt caggacgcgg
    14941 agctatcgga aaaacagcgc cgggaaaggc aagactacct cgatcaggtg caagcttaca
    15001 tccgagacct ggcccccaac gattcagggc aaccgctttc acccgacttg aaacgtcagc
    15061 tgatcgccga gttgcaactc ctgcaaagag aacaatctcc tccagacctg gcttccctga
    15121 tccgcaaaac gatgccggaa agagattttg accgcgcgtt gatcgagatc agactggctc
    15181 tgggagattt atctccggat acggacccgg ctttgtcaat atctggcttg ccggtaaact
    15241 tcgcggccaa cgtcccgggt tatcaagctc cggggattac tcagcccagg ggagaaaacc
    15301 ctgaagtgat ttgcgttgac gatcccgaca gccgtgatta cgatgacgct ttcagcgtgg
    15361 tgaagacagc cacgggatat agaattggag tgcatatcac cgatgtggct gccagtctgg
    15421 tccctggctc ggatctcttt gaggaagtgc tggagagagt ttcttcgctt tatctgcctg
    15481 ccgaaacggt caatatgctg ccagactgtc tggcggaaga cctcctcagc ctcagagcgg
    15541 gagagcttag agcggtgttg tcattttatt gcgatgtcga tcaggattca ggcctgagag
    15601 actggacgtt tctgagggaa acaatcaggg tgggcagtaa tatcagctac gcggaattgg
    15661 atgccaggct cagccggggt gaatatcaga tggtcaaagc tttttgcgac gctttgcgat
    15721 cagagcgtgg agctacaagt caaacatccg ggaatgacct gctttacaac ctcagagtga
    15781 gtggcagccg cgtcagattc aagagaattg atttcagcag tccggcccgc gtcatgatcg
    15841 aagagctgat ggtgctctac aatcgtctga tggctgcaat ggccagcgaa agcgatattc
    15901 cgtttctata ccgcaacatc agcagctatg aagacaatgt tcccaacgac gacgaagatg
    15961 ctcctgatca agccgttatc ccctcccaga caggttctca agcctatatc tccacgcggc
    16021 cgatgtttca tcctgggatc ggtgccaaag cttatctgca tgccagttca cccatccgca
    16081 gagtggtgga cctgatcaac caatatcagt ttcacgcctg gctggaagga cggccagcct
    16141 ccttcagtca ggaggccctg gacactttgg tgccagctat cgggaaacgc cttctcttgc
    16201 aaagagaggt ggtgagacag agcgagagat actggctgct gaggtatctg aagcaggatt
    16261 ggctgcaccg gcctctggac gcggtcttta tcaaaacctc ccgccgcggc tgtgtctttg
    16321 agcttctacc ctggggaaaa cggatcatag tggattgcga tatcacccct cctgtctcgt
    16381 atgagttgaa actgctcatc accaccgtgg atttgacagg ccagacctgc cgggcagagg
    16441 tgattctcta gcttccggat gttattacgg gtgcattacg ggtccgaccc gcaatgcacc
    16501 cgtaatggac ccgcaataga aagcggaaag atctatgtaa gccccccaat caatgctgtg
    16561 aagtccttcc cgggaacttg atgtgatcag tcttggcctg ctttgctccc caagcagtgt
    16621 ggccaggggg tcaccagagc ttgtccaacc catccccaca aaattcggct tgggccggag
    16681 ctgctatcgg caagcccagg attgatgaat aaggaagcta tctgagcagc agcaccttct
    16741 ggaaagatct gagctcggcc gagcggatca gatagatgcc ggtagcacag agatttcctt
    16801 gtgagtcctg accatcccag cgccaggtgt ttggcgagga atgttcaaga tcgcggacca
    16861 gctggccgcg ctggttgaaa acctgcaatt tgctgatagt cttatctttc gagctgatct
    16921 ccagattgct gctgaagggg tttggatagc agctcaaacc aactggggga ggcaggatgg
    16981 gatcgtcagt agctacggcg tcaaaatcgt acatgccgat aaaaccgctc tggatggcat
    17041 ccgaaccact gttgagcagg atattgccca gacgcatgct gccgttgtac catccacagg
    17101 tgatagcctt attcatcaga gctcgtttaa catcggagac cacatcggtg ttgatactgc
    17161 caaatccgac cgccgcggct tgagggccac ccttgagcaa agcgcagtcc cagacgccat
    17221 tggaattcag gatcgaactg ccaaaatcca cgctgttctc atatgatgcc actgtcaggc
    17281 tgcccatata gtcatgcaca tccacggcgc ggcctttgtc tgaaccggtt ccgccccagc
    17341 gctgcacatt gatccaggta ccgtttccgg gtgaaaactc ggccaggaag atatcgtccg
    17401 agccgtccac actgctgatc acgtggtcgc caaagacggc tgtgccggta actctgccgg
    17461 tcaccatgcc acggtattga agcaggtctt gcttgccgat gccgatattg ataggtcccg
    17521 ctcccaaagc ggaactgacc cacataatgc tgccatcata ggaatacttg gccacaaaga
    17581 ggccatcctg gctttggctg ataccatcaa aattggcccc ttgaccggcc actcctgtca
    17641 catatatata gcccgtttcg ctgcaatcca ccttgtagcc gtaatccacc ccctcgtctc
    17701 cagcgtgttt agcccagagg caggttccct gaggatcaaa ttttgccaca aacatatcgc
    17761 tgccgccata agaactgatg ctgatcccgc ctccaaaatc gattgtgtcg gcaaaccaac
    17821 cggccacgat cacgttgtcc tcatgatcga cagtgatgcc gtaaccgatg tcgttgagtg
    17881 tacctccaaa gcgtttcagc cataaaacat ctccaccggg gctcagtttt cccaaaaata
    17941 cgtcccacat gccagcggaa gtcaaaaccg tggaccccac gctcatcaca tcattaaaat
    18001 agccggtcag atagcagtta tcagttccgc cagttcccgc ttcaatagac aggcacacat
    18061 cctcgtcggg agagccaaac tgcctgaacc acagaggggt gccttcgcgg ctatacttag
    18121 ccacaaaagc gtcagacatc cccgcgctgg gataatctat cccgttaaag ctcatgctgc
    18181 catcgtattg gccacccacc catattgccg ggtttaaact ggagccgccc aaatcccatg
    18241 ccctcaccat gccagtggaa atgaggctgg aagcccaagt ccaggctccc gcgtgggcaa
    18301 ttgttacgca taatgttatc gcgcacagca gtataagctt tttcatagtt gtaactcctt
    18361 gagaggctgt ttgattatta aaagcaggat ttccgctggg gggctaaatg tcaattacta
    18421 ctttatccgc gtgagtaaaa gccggattgg caggtcctgg cggttggaca gccaaaacct
    18481 ttacagacca gctttgggac ggactgatgc cttttgtgct tgacattaaa agccctccca
    18541 ggatatcggc ttagcttaga aataacaata aaataatgac tggaggaatg tcatgtccga
    18601 caccttgaaa tacggcaagg tcagttccaa agaatcgatg gaactggaag aaaaatacgg
    18661 agcgcataac taccatccac tgcctgtggt tttggccaag ggcgaaggtg tgtatctctg
    18721 ggacccggaa ggaaaccgct attatgattt cctttcagcc tactccgctg tcaaccaggg
    18781 tcactgtcat cccaggatca ttaacgccct catcgaacag gccaaagagc tcaccctcac
    18841 ctcccgcgcc tttttcaaca acaaacttgg cgagtatgag aagtacatca cagatttttt
    18901 cggctacgac atggtactgc cgatgaactc aggcgccgag ggagtggaaa cggccctcaa
    18961 gcttgcccgt aaatgggctt acaccaaaaa gggagtggaa aaggacagcg ctatcgtagt
    19021 cttttgcgaa aacaacttcc atggccgcac tctggctgtg gtatccgcct ccactgatcc
    19081 cgactgctat gaaggctttg ggcctttcct gcccggcatt gccaaagtgg cttacaacga
    19141 cgtcaacgct ctgcgtgaat atctgaacca gcacggcaaa aacgtggccg ccttcatcgt
    19201 ggaacccatt cagggagaag cgggtgtgat ggttccggat gatggctatc tgaaagcctg
    19261 ttacgacctc tgcaaagagc ataacgtcct ctttatcgcc gatgagatcc agacgggtat
    19321 tgcccgcacc ggaaagctgc tggcggtgga ttacgaaaat gtccgtccgg acatcctgat
    19381 cctgggtaaa gcactggctg gtggagtgtt accagtttcc gccgtattgg cggaccggga
    19441 gatcatgctt accatcaaac caggacagca cggctccact tttgggggat ttcccttggc
    19501 ctgcgcggtt gccaaagccg ctctggaagt ggtcaaagaa gaaaaactgg ctgaacgcgc
    19561 ctacgaaatg ggagagtatt tcagggcctt gttgcgtgcc atcgatcacc ccatgcttaa
    19621 acttgtgcgc gggaagggac tcctgaacgc catcgtggtg gaacccaggg atgggattga
    19681 agcctgggat gtatgcctga tgctgaaaga aaagggcatc ctctgcaaac ccactcaccg
    19741 tcacatcatc cgtctcgctc cacccctggt gatcaccaaa gaacagctca aagacgccgc
    19801 cgacatcatc gcgggtgtat ttgccagtct gtaagctggt gcattgtaaa ttgataacac
    19861 tactcaaccc ggtagatcag gcccggtctg ccgggttgag tgttttaatc cgcgtgggcg
    19921 ggattgtgat cctgccgcgc ctattcaata aggaacaaaa acatggatag tagctttcgc
    19981 agcggatttg tggccatcat tggaaggccc aataccggaa aatcgacttt tttgaaccgg
    20041 gtactggggg aaaagctgtc catcacgtca cctaaacccc agactacccg ttacgccatc
    20101 aagggtattc tcaaccgccc cgacctccag atcgtgttta tcgacactcc gggatttttg
    20161 aagccccgct acgaaatgca aacccgcatg ctcaagatct ggagcgacgc tttcaagaat
    20221 gtggacctgg tgctctttat caccgacttg aaagatttcc ccaccgaata cgatcacgag
    20281 gtgctggaga tcctgaacaa aagccgggct catcagattg ccgtgtttaa caaactggac
    20341 ctgcggcccg agattgacag agaagccctc aaagcccttc tgccggccag cgttgacgag
    20401 gcccacttca tcagcagcgt caccggcgaa aatctggacg cgctgatgca gtccatcata
    20461 aacttcgtgc cctatcacga tccctattac gaggcagacc agctttcgga cctgcccacc
    20521 cgtttctttg ccacagaggt gatcagggaa ggtatcttcc acatctttga gcaggagatt
    20581 ccatactcga ccgcggtggt catcgaaaag tataccgaac tgccggatga agtggtggtg
    20641 gaagctcaga tctggctgga acgccagagc cagaagctga tcgtggtggg ccgcaagggt
    20701 gatggtctcc gcaagatcag agaatacgcc gaagctcagt tgagcgcgtt tctggaccag
    20761 ccggtgagag tgcatttgtg ggtcaagata gttcccaatt ggcgcaagaa aaacagcagc
    20821 ctcagggagc tgggctttgg ctgatgaatt cagttgacac atttgcggac tgagtaatta
    20881 ttgttgcctg tacgttctgc cattcttttt catatttcca aataataagc tgagtgagag
    20941 ttatgcgtca tataaaagta cccggcgcgg gacgtgaaca aggggcagat gaagccatgg
    21001 cttctggtct ggacacccta ttagtggtcg atatcggcaa cacgaatatc gtgtgcgggc
    21061 tttttgtggc cagagagctg atccgcacgg cccgctttca tagcagcgtg aaccgtactg
    21121 ccgacgaata ttacagcctc ttgatcccgt ttatcaccga aaccggggat ataaacatca
    21181 ccgatgtggc catcgccagc gtggtgcctg agcttacccg catgtggcaa cacctcttcc
    21241 agcgtcactt tgagctatcg gcaaaaatca tcacggccct cagtcctttg ggattgcaat
    21301 acagggtggc tgatcctggt tttatcgggg cggacctcgt ggtaaatgcc tttgccgcct
    21361 ggcagaaata ccactccagc acgatcatca tcgatttggg caccgccacg acggtgcaat
    21421 ttgtgaccga caaaggcagc tttgaaggca ctgccatcat acccggcatg aaaaccggag
    21481 cccggaattt gtttgacaaa gccgccctgc tatctgaaat cgagatcagc gctccgccag
    21541 ctttgatcgg cactacgact tcagcggccc ttttgtccgg gatcgtcaat tcccatgcct
    21601 tcctgctgga gacctatatc cacaagctca agcagcaata ctttgaccac aagcgcatcc
    21661 tcacaattct cacaggtgga atggctgacc tgcttaaacc cttgatgccc agtgtggatt
    21721 gcgtggataa gaccctcacc ctggagggcc tgatgctggc tgccgaaaag ctggccgcta
    21781 ccgtcccaac ggagcaactc agaacttaga atatgatcaa gcgaactctg gcgatcgctg
    21841 tatctatagg gatcttgtgc atcctggcgg ctaacgtcat gccgctctat ctgcacctgc
    21901 ctgggtacca tcccctgatc gacaatcagg tggatgtcta taagctggac aaggggatag
    21961 gttttacaaa aagccgtcag tatctgattc cctcaatagc tccggtcaaa actgaaacag
    22021 tggacctgcc caaccagaga gtgattatct ccacctccat agacgaactg aagatcacgg
    22081 atgacagggt ggtttctttc gacagctatt tccgctcact gcagcgtaaa gcgtttcgca
    22141 agagcctctt cgcccaggtg aagaccaaac ggcagcagac agtggtcacc acctctggtc
    22201 tgatcggtga attcaagctc gaactgccag ccatcgcgat tcccaaggcc gtacaaaaag
    22261 ttctgggctc ttcggcggga cggctcaacc tggatggtac gcaaaagatc accatcgagg
    22321 ctggcagcac caaacgcaaa ctgcagccta tctatgagac gcagtccagc agccgtttcg
    22381 acctcaaaat ggagcaggaa accaacctgc gcctttccgg caaaatcggt gacaagatcg
    22441 ccgttaacct caagtataac agcaaacagg acgagcaact ctttgacccc aacaacgtca
    22501 gcatcaaata caccggcacc gaagatgagg tgatccagtc catcgaggct ggcaatatca
    22561 ctctggccct ttcgggatcc cgctatatat cttattccac cagctctcag ggcctgtttg
    22621 gcatcacctc caagctcaaa tacggcaata tggatctcac cctgatcgcc agtaaggaag
    22681 agagccaaaa gaacactcaa agttatatcg gtaccagcca ggcggattcc accatcttcg
    22741 acagcaagca atacgtggcc cgcacaatgt attatctgga caggcccgac agtctctatc
    22801 agctctatca ggaatctgat cccgattgca agactggcta tgccggcaac gccatccgca
    22861 ccggtgccaa cggtgcctgg atgatcagca acgctgatcg cctgcccgcc aatgggacag
    22921 tgagagtctt tatcgacgat ctggtggcta ccaacaacgt gggttccgct ttgggagata
    22981 cgatccagat cagcaatcag gattatcagt taaactggga tgaactgatc gagggggcgg
    23041 attttgtcac ggactacgac tccggtatca tcacgattaa ccgcaatgtg ctgacctccc
    23101 atgtggtggg agtcagatat accaccagag gtggagaagg cattacgcca gtaagcgtgc
    23161 ctgccccgag tgcagaggag ttggccaatc ctgatctgct ctacaccaag atcatccgca
    23221 attacaacca gcaatacagt cccgatgatc ccaacagtgt gtgggactat caaatgcgca
    23281 acatctacaa gatgagcagc accaacatcc gcaacgatgg tttcagcctg gatatctata
    23341 ccgaaaacgt ggaccgtacc cgcaactatt tcgtggcgga tagcctccag gtggggcaga
    23401 acgtaagtta ttcagactat ttacgcatgg acagcaacgg cgattccaag gtcgacggca
    23461 gcgataccac cgtcaatctg gtctccggtc tgatcatcat tcctatgctg aggccctttg
    23521 aaccgctggg tgatgagatc atctatcaga tggagaacga ttcctacagc atcaatcctc
    23581 tggacacgga gctttttatc gccgccaaag gcaagattgg acgcgacgcg gtggacctgc
    23641 agcaagggaa tatcctcaag ggcagcgtga gagtaaaagt caacggcaac gagctcaagg
    23701 aaaacctgga ttatattgtc gattatgact ttggccgcat cacttttctc actcccgcgg
    23761 gcaaggaccc ggatgccaag atcgagatcg attacgagtt tcgagggatg tttgacgtgg
    23821 ccagtaaatc gttggccggc gtgaggatgg attggaatct caccgattac gccaagctgg
    23881 gcggcacctt tatctatcgc agcgaaaacg tggcggacaa gcgtccccgc atcggcaatg
    23941 aaaacatcga aatgatgctg gctaacctgg atggcaacat cacattgaag cccaaattca
    24001 tcacccgctg gatggattgg ctgcctttta tcagcaccac cgcggaatcg agattgactc
    24061 tttctggcga gatagcctac accctgccaa acatctacgg cgatccggac ggcaaaaaga
    24121 actccatcta tttggacgat atggaatcga tcatggattc ctatccgctg cctgtgacga
    24181 tctcatcatg ggtgagaggc agtaaaccct ggggcaccac tttggccaag gggataccca
    24241 actggtacaa tcccaaaaac gtgaaacgct cagaactggt ggacgatccg gaaacgctca
    24301 cagaccggga aaagaatgag tttgtcactg tgctggccgt caaaaacact cccgccaata
    24361 tcggtgtagg cggatcgaac gtgcagagct gggcgggcgt gatgaagtat ctgggcaatc
    24421 agctggattt ctcacagaaa aaatacatcg aagtgctggt gaaactggat gtcaaacagg
    24481 gcgaaacggt tccggacccc gtgatcaaga tcgacctcgg tgacctgaat gaggacttct
    24541 acaccgaata cggcggtttg ggagtattga acaacgaaga cctgaacaac gacggcgttt
    24601 taaccctgga tgaagatatc ggtctggata tgaaagaccg tgtaacatct gggtccgatc
    24661 ccaacgatat ctctccctct acgatccaga atcatcacta tgaagcgtat cccggcatca
    24721 atggcacgga aggcaatcgc gcgctcgaca ccgaagacct ggatggcaac ggtatcctca
    24781 atcaggtgga tcgttatttc agctattcct tcgccatcac cgggacacag catttggtga
    24841 atataaacca cagcaactgg aggctttacc gcatcccttt gcacgacccc aatgtctata
    24901 cgatcgtcaa taattccgct gccggagtgc tgccctccat gcagaggata tcctttgcca
    24961 ggattactgt ggagaccgat attccggccc ggatctacat tgccgatatg gccatcgttg
    25021 gcaataaatg gcaggatttt ggggtgcgtg acttcaacaa tacccaattc caggaagatg
    25081 tggtaacctc acttggcacg acctatctat ctggaatcgt aaacaatcag accaacagcc
    25141 gttaccgtcc tcccgaagga acggtttaca tagaagaccg ccgtcaatcc gtggaatcct
    25201 ctcttaccct cgatgtcaag aaactgcagc ccggcaatca ggtgctgctg aggcagcgct
    25261 ccactgaggc ctacagcttg ctgtcctatg gcaaattgaa gctgtatgtg tatcccgaag
    25321 ccttgcgcac cggtgaagag catcccgaca aggtggattt cgtgctgcgt ctgggagcgg
    25381 atgaatttac ctattacgag atccggcagc gggtgtccat ctatgattgg gaaaccaata
    25441 tggacaagga cagatggctg ctctatacct atcagctgtc tgacattacc aagctgagag
    25501 aggcgaatcc cgataccact cagggattta tccaggtgga tgaaaacacc tacttcagtt
    25561 ttagaggccg gccaaccctt tccaccatca gggatgtctg cctgggagtt tccaatccct
    25621 ccggaagctc catctacaac gggatgttct atttcaacga gcttctgctc acggacccat
    25681 atgaagatgt gggggtggca cagcgcgtta cattgaatgc cgcgctggcg gatttcagca
    25741 ccctggatgt ggactacgag caaaaaagcg agaacttcaa tcccaatatc caacgtggac
    25801 gtgccaacac cttcaccagt tccacctcac tcaacatcac caataagtat ttcctgggca
    25861 agctgttccc ccgcacctgg gatgtggata tcccgattac gctgtcccgc aattacaccc
    25921 tcggtattcc tcgctaccgc gccaattccg atctcctgcg cgaaagcatc gatgcggaca
    25981 gcaccaagct gagggaacgc aatgaaacct tgctctacgc cgctgacttt gccttcagcg
    26041 tcaagagccc tcccaaaaac aagattctgc tctacactct ctaccgcagt tccttgagcg
    26101 gcaggataga aagctctgta cgcaatcaac ccaccacgcg ggacagcacc ttcacgctgc
    26161 gtggcaccta taactacaat atcggcttcc cgtctgagaa ggtgagcttt aaactattta
    26221 acaattaccg tttgggattt ttccctacca cctggaacaa cagcctcacc tacaacagca
    26281 ccacccccag aagcgaaaac tgggaacggc gccagataga tggagattac atcacctact
    26341 ggttcccgcg ccagggagtg ttgccaaccg agctcctgac cacagattcc aacctcaact
    26401 gggccctcac cagtgacata ggtctcactg cccgtcttaa taccaagcgc gatctcatcc
    26461 aaaaacagct gtgggaaaag atcaatgtgg ggaagctaac tgaatacgtt caggatttgg
    26521 gcctcaacta caatcccaat ttcctgcgca gcgtgatgaa cctcacggcc agcggcagta
    26581 ccagatattc tgagctgcag cgccgttatt atgacaacag cagcggccag caagtggaag
    26641 tgtatcaaag tgatggcggg gtaaaccgaa ctttcagggc caacctcact ctgatgaatt
    26701 ccaccctgct gggtagatgg gcgcagaacc tcactcaaaa acatcaagct aaagcagacg
    26761 ccaaagcaga cgccgataaa accacagaca aggatcctgc tgataagcct gaagataagc
    26821 cccaggacac tgacgaagag accaagcgca agaaagaaca ggaaatgaag gatgaagagg
    26881 cccaaaagcg ggctgaagaa gaaaagcgca aagccgaaga agcggcgttt tgggacaacc
    26941 agcccaaaga cgatcccttt ggcggaacgg gagatggagg tttcccagat ttcaaggacg
    27001 gtgagtttgg ctttggagat ccaaaagaag gcggcatggg accgggggct tcctatcagg
    27061 gatccgatcc cggcagccca tacgtgcctc ccaaagatgt caaatatctg ccagccttgg
    27121 tgttaggcta cctctccaaa ctcaagaaca tcactgtctc ctatcaaaac ggctatacga
    27181 tgaactacct ccgcaaggaa gaacggccac cttttgcctt ccagatcggc ctgccgcata
    27241 ctgtggaccc aggatttctg gatgccaccg gaaacgataa caccttcacc ctctccagcg
    27301 gaatcatgat cagccgcaac atcgacagcg tgatcaacta ttcatacacc atcaacaagc
    27361 gctactccag cgccagcaac caaaacaccg cgatcacttt tccggatatt accctcagtc
    27421 tgatggactt cgagaaatgg gtgggactct ccaaatatct ctccagcacc cgcatcaaca
    27481 caggcttcca gcgcagcatc aggtccagcg gaaacatcga ttgggtcaag cccaaacagg
    27541 aaaccaacgg gatcgccttc aatcctctca tcggttttgc cagcacgctg tttaagaaag
    27601 tgagcaccaa tctcagtttc agctttgccc aaagcaccaa taccaccgac atggagagct
    27661 acgacgtgat caagaagacc aacacccaat cactcaacgg caatctctct tattccttcc
    27721 gtggcggtcg cggcttcact atccccttca ccaaaaagaa gatccacatc aagaatgagc
    27781 tcacttccag cctggcgttt gtgtatgaac gcaactttga cgataccacc ggccgcgaag
    27841 gcaactatca ggtggaccgt gatactaccc gcctggcatt cacaccgggg gccacctatc
    27901 agttcaatca ggatatccgc ggaggcctta ccggcacctg ggaaaacacc agcgacaaac
    27961 gcagagatga cggagtgagg attttcagga tcggagtgtg ggtggaggta aatctgtagc
    28021 tcactacaga ggtacagagg ttaatctttt tcactacaaa ggtacagagg aaacagagag
    28081 agatacggag attattttca ccccagaggg cacaaagtac acagaggaag atagaacgca
    28141 gatgacgccg attagactga tttacgctga tattgttgat tcacaaggta agaccgttga
    28201 ataactggta tcactggctt tagagcttgc gcgaaaatcg attttcgggg cagcattcct
    28261 tgagagtccc atcggaactt cagtttagat agacgcggaa gcccaaactg agctgagtcc
    28321 cagcgggacg ccagtttaga tagataaaca tatcagtttc catatcatta tcatcgtgat
    28381 atcttgatct aaactgccgt cccgatggga ctcgtatctg agacctggga atcgattgtc
    28441 tatctaaact ggcgttccta cggaactcaa agcgccattt tcgcacaagc tcttcaaccg
    28501 gtcccagcgt cggctttagc cagtgttacg accggctaaa gccagtgcta cgaccggcta
    28561 aagtcagtgc tacgaccggc taaagccagt gctacgaccg gctaaagcca ttgctacgac
    28621 cggctaaagc cattgctacg accggctaaa gccagtgcta cgaccggcta aagccagtgt
    28681 tacgatcacg cgcaacggcc tcaaagtgta aacaagtaaa gtggttttgt attagaacgc
    28741 gataaagatt agccgggtgt ggacattaca acttgaaaac tcgaaaaccc cacaacttga
    28801 aaacatcaaa tcgccaaaac gaaacaactc taaaacttta aaaccctaaa actctacaac
    28861 ttgaaaactc gaaaacccca caacctgaaa acatcaaatc gccaaaacga aacaactcta
    28921 aaactctaaa accctaaaac tctacaactc tacaacctga atctacctta tcagcactat
    28981 cttccctctt ttgctattgc caacatcgtc cgtcaccaca aagaaatata ctccgctggc
    29041 acagagcttg ccgctggaag tcttgc
//
LOCUS       DAOSFN010000012        27279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1375_length_27279_cov_8.000808, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000012 DAOSFN010000000
VERSION     DAOSFN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 27279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27279
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1375_length_27279_cov_8.000808"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLO35_01805"
     CDS             <1..299
                     /locus_tag="PLO35_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78425.1"
                     /translation="LAVALTECLLTHGSLGAAIQIEGTPNFLGLGFGEVQSAVVVSVR
                     QELAAAVEKIAGQYSIPLLRLGEVTEDATLKIGSYIDLPLAGLRQAWEDGLKLN"
     gene            382..942
                     /locus_tag="PLO35_01810"
     CDS             382..942
                     /locus_tag="PLO35_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78426.1"
                     /translation="MGLFFGNLFWGILLVLWGISLVLKSFGVSLPLARIFFAAIIILF
                     GVRLLMGGSWKARSSGINTRSVKGDKIEYTTLFASQNIDLKQIRPGHKPIEVTVVFGS
                     AYVELPADVAFEVEPTTVFGSLITPRQSHFGFSDDREIFNADSGGEKVFIEANAIFGR
                     LEFVVKPVDRPQPAAQPDSTGSGIGI"
     gene            945..3122
                     /locus_tag="PLO35_01815"
     CDS             945..3122
                     /locus_tag="PLO35_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH78427.1"
                     /translation="MRHTFLRSGMTFIILLLGITWLGANQAVGYAFSGGGARGYAHIG
                     ILKVLEENGIRPDYITGTSMGAIVGALYAIGYNAAQIEELMLRLNWDSLLDDVYPRKQ
                     LYIGQKRWAPYGAVNLEIGNNWQPRLPSSVWVGNGLNLELARIFAPAGDNMDFTALPI
                     PYACVATDLLSGEPVIFSRGNLMQAVRASLSIPSLIQPFELDGHIYIDGGVSQNLPIK
                     EVIGLGADYVVGVKTNSGLRSRNELGNLIQVLDQTINIGMTRNLNAGLEDCDLLLEPE
                     LEGYHSTDYNLAAQIIAAGEAYARDNLDRIVAFRQQVEAQGPKIWRLAGPLPSPGTKT
                     LTSISCTGNDHLSLAKIRDYMGITIGGDYSADQLVDACRDAWHSQMFHTIYPVIATEG
                     ETYKLRLFVKERTRRQLGIQLSYSSEEGPLAGGILKLNNILLKNSILQAALTMGGKTE
                     VNLDYVKNFGELWGSYFRVYSYFSENRRYYYDESFNKVASVKAAELGLSGGLGIFANK
                     LAVAEAFIYGFRTRLYRDISTVAGIDSLYLISGAGVKLYHESLDDYYFPSSGFRLSLK
                     FNFARSSQLSDYIYSKVHCNAALYTPFNPWLSLKTALDLGSWFGKQQINIDPFYLGGS
                     NGYLGYQKYEISAPVYKTFDVGLTAEPQRNIITSLGVQGLSIGDVDDWSTDQKLEGSL
                     YLAVGYRTALGPLRFSVAFREKSHPNYYLNLGYDLDVFHFSRR"
     gene            3222..4082
                     /locus_tag="PLO35_01820"
     CDS             3222..4082
                     /locus_tag="PLO35_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78428.1"
                     /translation="MRGISLALMIMVLGLGILFAQAPDMSALIATLKLAQSGDAEAQW
                     QMAGYYEQGLGVEPDQQEAIRWYRQAATSGHVEAQYKVGAFFSSNTEEAVQWYEKAAL
                     QGHLEAQIKLGNYYNTTNGHKKNPAEAFKWYSLAARQGHPEALYEVGNAYYYGEGVAK
                     SHKEAFNHYKKASDGGHADAAISLGDCYFNGEGTQPDYAQAFKLYESQAKKNNPHALY
                     SLGFCYESGKGARMNIIRANAMYQLAAHYCTSGNKSTFESYAQKTSARLSKVDQEKAD
                     KIRDEWLNKQ"
     gene            4064..5149
                     /gene="hydE"
                     /locus_tag="PLO35_01825"
     CDS             4064..5149
                     /gene="hydE"
                     /locus_tag="PLO35_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOH78429.1"
                     /translation="MAEQAVKTRPDKAEIAALLSLKSPDELKALFARAYEVKKRYVGT
                     RVWFRGLIELSNICSKDCFYCGIRSGNASVTRYTISPDEVVKEARWCFEQGYGSIVLQ
                     AGERSDAAWTEIITDLILKIKALSDGKLGITLSLGEQSEGVYRQWFEAGAHRYLLRIE
                     ASNPTLYKQLHPADHSYDARVECLRYLKKVGYQVGTGVMIGLPGQSVDDLADDLLFFY
                     DIDVDMIGMGPFIPHKDTPLGHLVPSFDPDEAIDMGLKMIAACRIFLKDVNIASTTAL
                     QALKDDGRELGLLAGANILMPNITDTSFRDKYQLYEGKPCMDENASLCRGCLERRIQA
                     IGETIAYNEWGDSVHFRTRTQTINQAD"
     gene            5248..5571
                     /locus_tag="PLO35_01830"
     CDS             5248..5571
                     /locus_tag="PLO35_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOH78430.1"
                     /translation="MFPGGKGMQDLMKKAQKMQQEMERNQKEMENTIFEASSGGGMVK
                     VEMTGKGDLKSLKIDPQAVDPDDVEMLEDLIVAAITEAQTKVNEASASMMQGLTGGIK
                     IPGLF"
     gene            5576..6172
                     /gene="recR"
                     /locus_tag="PLO35_01835"
     CDS             5576..6172
                     /gene="recR"
                     /locus_tag="PLO35_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOH78431.1"
                     /translation="MLQSPRLDDLITALNRLPGIGRKTAQRLAWHLIGGEKSRALELA
                     NAIISAAESFAPCKVCNLLAETDPCQFCSSVDRDDSQLCLVENNADVAIIENMHEYRG
                     RYYILGHLLSPLDGFGPEQLGIHKLMKLIDDRAPDELILALKPSAEGEATIHYLTETL
                     KAKPIRITRLSTGLPFGGDLEYSSILTLSNAWKRRYPV"
     gene            6177..6803
                     /locus_tag="PLO35_01840"
     CDS             6177..6803
                     /locus_tag="PLO35_01840"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOH78432.1"
                     /translation="MFTGIIEAVVPILAVREGSGRRHIKLSRPQAFDDLRAGASIACD
                     GICLTVLGFDQSSFEVEVMAETLKKTIAAGWRNGTLINLERALRLGDRLDGHLVQGHI
                     DRVLTLSSTRNQSDTLYLYFHYPREDAALLVAQGSVCLNGVSLTIAELKTDSFAVALI
                     SHTWQNSNLPALKPGSRVNVEYDVLGKYILRSSNPSKLSEEWLREQGY"
     gene            6787..8070
                     /locus_tag="PLO35_01845"
     CDS             6787..8070
                     /locus_tag="PLO35_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH78433.1"
                     /translation="MNKATRLACILFLAFMMMSCGSKKNPTGGPVDTVKPEVLATLPQ
                     EFGQLTDRIEITFSKAMDKSSFTEGLYFYPPISARKISYSGRTLSIRIEEKLKDDTNY
                     YLTLSTRIKDTRGNPIAQSRSFVFSSGRLSDLRLGGKIDYEESADQGKPVTIDVFSAD
                     SLLVLSHTANGASYGIETLNPAVYLLKAYQDKDANGRYDLGREAWFEGRVDLKRSANL
                     DLMMSYNDTTLTVIKDARAISDRKVELTLSKVPVSIGAVSVRAGNGIRLPVLISELEG
                     IRLTLITQAQDSLDYRVEISDLKDVRGNRNPKSGIGFKGSIIKDTEAPIVLSSTPRNG
                     TSVSNLLPTITVNFSEIIPLGNMKARLLASDQNKEIPLQILKSDSRSYSFQPSQPLSN
                     YRSHSLVILADTRDSTGNPLGKDHVISFLPLLKTR"
     gene            complement(8273..8836)
                     /locus_tag="PLO35_01850"
     CDS             complement(8273..8836)
                     /locus_tag="PLO35_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH78434.1"
                     /translation="MKIAVWILFALLALFPIAMFGQTDISYGAKTGFALSLHYGTKAD
                     PQDLKVKLAMRPGAYAGAWLDLNILPNLALGYELLYAQKGSRIKIDIFRMENEFGEME
                     ELAKPAEMNVKYYMDYIEMPLLFKVKAIDRPKWSMSVITGTAMGFRINGDYELDGIFY
                     LPDGDEFDEVEVTDSNNMEYVNIFDFS"
     gene            complement(8833..9600)
                     /locus_tag="PLO35_01855"
     CDS             complement(8833..9600)
                     /locus_tag="PLO35_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH78435.1"
                     /translation="MLAKIKTYLWYALLIFFMLLCLFALPVYLLGKWLLPPQRYLKMT
                     LFARYFWGRVVVRSTGSKVSFEGLENIPTSTTRQHSVDSDSPSLRLCYVGNHQSYFDI
                     PAFLGWSGQPVGFIAKQELLRVPILSQWMRIINCVFIDRSNPRSAIESFKESAKVINA
                     GFPQIIFPEGTRSKGANMGPFHIGSLKLASMADAAIVPFAIKGSYRAYEIDGHIHRAR
                     IKIRLLEPILTDDPIYHDKLELAEQIRSRIAAALEEM"
     gene            complement(9792..11237)
                     /locus_tag="PLO35_01860"
     CDS             complement(9792..11237)
                     /locus_tag="PLO35_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOH78436.1"
                     /translation="MEKYPNIIILLNLPRLYIPKAEFVLRTYCYILRLNPKFVYGNHY
                     EGSHLYYGYPTERDYPIKINFRDGTADFFERRELYPLDLVDFCLYKNEYIPFLFSRGG
                     AIFSLDTDHCVIHKDLVAGGFYFLTCWNEYILNYHGAHKGRVDYRESLQYRWDFTEIP
                     VVDVYCQILLWAVGQFIPEFVREIYWSEDKDFCVSLSHDIDYWNYWNTRQKFQTLFYN
                     LRTIPRRPLNALWKILGHGLHKNLLHNPWRNVKRIIRQETDLGVKSTWFLLARDDFED
                     QRQNYFSDPVSKEQIIDLLGQQEVGLHGSPESAFDPVVLQAELDKLRELGFDPKGFRT
                     HYLHFDYQKSFTILENAGIHFDSTLGYWENIGFRAGISFPFFPFNIAENRPFRVLEIP
                     LIVMDTTLRSPKAMNMSPAQSRRVLYRLIDLAARYQSHLSLLWHNTSFDPVDFPMLGR
                     LYWKTINHAVKRRAWVTSLNDVYEEWVTMSY"
     gene            complement(11239..13644)
                     /gene="lon"
                     /locus_tag="PLO35_01865"
     CDS             complement(11239..13644)
                     /gene="lon"
                     /locus_tag="PLO35_01865"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOH78437.1"
                     /translation="MEDHNSRIPRTLPVLHMGNVVMFPFLLMPLVVADDEGKLVIDHA
                     LSNDKSMAFFLDQEGEDAGLHEIGTAVTILRMLRNQDGTISLLLQGTSRIRLQKVTQR
                     EPFIMADVESVTELNIEDTEVHAYRTVALELLEKISQEGNLLNREMISGLGNIKQAGR
                     VADIIAGNLELQLADRQRILELIDLKARYRFLNDCLAELIKQMRVENHIRSNIQLEMN
                     EDQRRYYLREQLDAIRRELGESDEVSKEILKWRELIEKNDLPDEVRDTALEELERLAS
                     MQPASSEYGVIRNYLDWIVNLPWRVYSKDRLDLVKIDKVLTKDHYGLEKPKERVIEYI
                     AVKKLKGSIKGPILCFVGPPGTGKTSIGKSVARALNRKFIRMSLGGIHDEAEIRGHRR
                     TYIGAMPGKIITEIKRQGTANPVFMLDEIDKLGRDFRGDPASALLEVLDPEQNNSFTD
                     NYLNLPFDLSEVMFITTANSLETIPAPLRDRMEIIEFTSYLEHDKIEIARHYLIPKEL
                     EDNGLSSSIVKLQKSALQELIRYYVRESGVRNLQRRIGSIFRKIARKVASGEQLSLSL
                     KASDIASYLGPRKYSLELANRKPEIGVATGLAWTSYGGEILFCETTRMPGKGAIVLTG
                     LLGEVMKESARIALSHLKSHQQDYGIPTNSFDKWDIHIHFPAGAVPKDGPSAGITLTT
                     ALASLFTSKPVKHDIAMTGEVTLLGKVLPIGGLKEKILAAKRAGIHKLIVPAENEASM
                     ADFDADILEGMDISYVDQVRDVLKAVLIGGLHVKPEPREPVKRNSGPRKTSNRRIEGG
                     TGS"
     gene            complement(13949..14692)
                     /locus_tag="PLO35_01870"
     CDS             complement(13949..14692)
                     /locus_tag="PLO35_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH78438.1"
                     /translation="MSHILEAAGLSKSFLLNGRSRLILEDASLALSGGEFLTIIGASG
                     CGKSTFLELLAGITRPDQGRVVFMGEDITARSGKLGYMPQDDLLFPWLSVRDNALLPA
                     RVAQKDLRQARKRLDELLPVFGLQDHASHLPWQLSGGLRQRTALLRTCMSEAKVLLLD
                     EPFASLDAITRRQLQLWLKEIASRLALSIILVTHDISEAIILSHRVVLMKGDPGRFGE
                     EYIVPAGLSDDNPAIDELKRDIFTALGIR"
     gene            complement(14689..15675)
                     /locus_tag="PLO35_01875"
     CDS             complement(14689..15675)
                     /locus_tag="PLO35_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH78439.1"
                     /translation="MKKALCILLLTALALGGLVSCKREAAQKAARKLKVVLDWTPNTN
                     HTGLYVAQELGYFKEAGLEVEIIQPGQNSAEQIVASGHAQFGISYQESVIRARIENIP
                     LVSLAAVIQHNTSGFASLKEAELTRPKHFEGKRYGSSGWPTETEILRSVMTADSASFD
                     KLKVVEGVTDFFSTIGRDADFEWIFWGWDGVEAQRRGIALNYIPLKDLDPVFDYYTPV
                     IVTNEKTAQADPELVKSFMAAVSKGYRYCIENSSSAADILIKAVPELAANAEQVKLSM
                     AYLKDQYQAEAPRWGEQRLEVWQRFVDWMFNKRMLMTGMKADQMFTNRFLPE"
     gene            complement(15908..16834)
                     /locus_tag="PLO35_01880"
     CDS             complement(15908..16834)
                     /locus_tag="PLO35_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOH78440.1"
                     /translation="MKGKSRNTEAFTPPLPAALVYLRDFVFHIRVEKGMAENSVVSYE
                     RDLRDFLAYAQKPVERIDHQEVIEYLTLLSDMGLIVSSLARKRAALNQFFRYLADNDA
                     NRVMDFDKVPKIKVGQHLPDFLDVDTMLRFLDSQPVQTSLELRNRLMMETLYATGMRI
                     SELLGLSLHDLNRSEGIILVHGKGNKQRYVPYVNSLEPLFDVYLNAHRPALLKMKRSD
                     YLFLNGRGGKLSRQGFWKILKEAALKAGIKQEFTPHTFRHSFATHLLEAGVNLRVVQE
                     LLGHASLNTTQIYTHVNTRFLVETHRLYHPRA"
     gene            complement(16914..18371)
                     /gene="guaB"
                     /locus_tag="PLO35_01885"
     CDS             complement(16914..18371)
                     /gene="guaB"
                     /locus_tag="PLO35_01885"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOH78441.1"
                     /translation="MKKIRKSYTFDDVLLVPQRSEVLPSEVDLSARITPRVQLRVPIL
                     SAAMDTVTEHALAIAIAREGGIGILHKNLSIADQANEVHHVKRAESGVVSKPYTVSPD
                     DTLLKVLELKKSHKIGGFPVVEKGRLVGILTNRDIRFETDPRRKVRELMTPRDRLVTA
                     PSGVSLEDAKALLQKHRIEKLLLIGADDQLTGMITVRDIMKRIDYPNAVQDSKNRLLV
                     GAAIGVTGDFLERAEELVRMGVDLLVIDTAHGHQINIAKALKKVKNKLGVEVIAGNVA
                     TAKATRFLIDAGADAVKVGIGPGSICTTRVIAGIGVPQLTAIMDCAEVAAQSNVPIIA
                     DGGIKYSGDIVKALAGGASAVMIGSLFAGTDESPGEFIIFNGRRFKSYRGMGSIGAMK
                     RGSGERYFQNATEENKMVAEGIEGMVPYKGPLKDYIYQLCGGLRSGMGYCGARDLESL
                     KVHAEFIEISSAGLKESHPHDIRITKETPNYQSGD"
     gene            complement(18505..18879)
                     /locus_tag="PLO35_01890"
     CDS             complement(18505..18879)
                     /locus_tag="PLO35_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78442.1"
                     /translation="MKKILKALLLVGGTALAAHFGMKAYKRLSGVAKLAKSLPEFLNN
                     VYGERPKLDINRSFNTIAIKIGYSQEIIDKHNDIETTVREYVDDFYPDLSKCSVDVSI
                     YVPGTEEEDLEEEEAETPESQD"
     gene            complement(18903..19619)
                     /locus_tag="PLO35_01895"
     CDS             complement(18903..19619)
                     /locus_tag="PLO35_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78443.1"
                     /translation="MKKLLFAMTILMGLATAAGALTIYDIQYTNTPGVDNSYPSPYVG
                     REVTLEGIVTGADYAQGGYFISEPISGSWRGIYIADKRNRPSQGDKIRVRGRVHESFG
                     MTCLLDISSYRMLDRNYGLPQPLIVTTGQLSRADEAEAYEGVYARVLSASVASSRSKA
                     GRFAVTDGSGQCSITTGSFSRQKALSPAAGTQFSSIVGIVVFGFSEFSLNPVAPGDTQ
                     IQQPLSTQNRSWGKIKSIYK"
     gene            complement(20170..22635)
                     /gene="sppA"
                     /locus_tag="PLO35_01900"
     CDS             complement(20170..22635)
                     /gene="sppA"
                     /locus_tag="PLO35_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOH78444.1"
                     /translation="MTPLRFVFILISLGFVLTDLGAQSVNVDPDSLEFPIASMDDQFS
                     PYGNPSLLGTGQISGLGYAQNFDNNDWIKQHWVFYNSDGFSYVYENRNDVSYHNFAVG
                     SPLSERGFLRNLYFGSRYSWRNSATNEGWWRNGLTFRPHNSASLAMVWDNPYRQSPAY
                     KAGIAIRPLSWFAPQHSYRLELSGDISYKKVADETNPALMEYDFTSPVLGFSTQIVDG
                     LKLGGSYNLDSKSALLNFSLRFGKTELGSLALKPDGKDPVLLGYVNAGDLNWKPFLGL
                     SGKKWYDMKLRGEIVSYKSPSYSFGPFRVFDSNQRGIDSVINELEKAAREPGVEGILL
                     INPSFSSSFALQQELVRAFASFKAKGKKVECYYDNISNGGYIFAASVADNIWLNPMGS
                     VDLHGLSITSPYFATLLDSLGVEVLNFRSHKYKSAGNQFSETSMTEAEREVYDAILQT
                     IYEQMIVQINNGRGSRINGKLEDVIDNGPYYLAQDAMAAGLVDSLIYQDQLEDKLKDR
                     YGFTARKGSLTSFAKYDWNIASPKQIAVIYASGNIVMGKGEPGTMIAAESTVKLIRAA
                     RKNPAYKGIILRVDSGGGSAQASDIILRELELAQTENKKPVVVSMAGMAASGGYYIAC
                     KADKIVAEATTLTGSIGVIGIAFNAEKMFNKVRVNWSTVKKGRNADFPSIYRKWTEEE
                     KQKMTGFIESVYEDFVGKVDTGRKNLDSEQVHAYAQGRVWTGKQALELGLIDDLGGME
                     TALIHMRDLTGIKGDIELVDATSSNKGIRIDMRGDSLPGLSTNGLLLELEKSYGHLYE
                     LWKQYSDQSALMLTPVSAPVMEF"
     gene            22849..26748
                     /locus_tag="PLO35_01905"
     CDS             22849..26748
                     /locus_tag="PLO35_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78445.1"
                     /translation="MKKPTLIIIALLLLSMAQALVVETGSFRDFLYGEEPACAYDNWI
                     SHIAEGIASANYNLYAPYDRQLNGFGDFRIINALETANWNALIGLILTESWDEAELMI
                     LAAGFPYQLVQFNDTDTGRTYYMLREIPDMAEIDDNNTADPYDDESGAFMYGWGLYIY
                     NPQGSRPVIITVPHPTDDFATPAMGALALEVWDARFMIISGAGREVKWTNVAPFTNSK
                     SLSDPTRVANHPFNFAYDKFADQIRSQYGLREFSAQIHSYDWNRHEGYPNNQISAGYQ
                     KLCPNLPIRDLSDLNNDMIHQAPHLVFPANTVGIHAPVYLSDYYSVNYSIHDFNWSDG
                     VNTYPVNDYVDLPAYSQNHQMLYTLSGWNDYDSYDPFFHVEMDELPNCYEQTENNYHW
                     FYGWDTAQQKWDYANLFGRYMQFYSPWVYHLEAVMDDMFQMNDGLTPTPPTELSVLNS
                     SLTHITLGWQPSSAYDFDTYEVLYAPEPIGTDNYQIFNRSNNSFLASQDAQSISVTGL
                     NNANTYYFKIRAKDKNGNYSELSNEVQSIPAPANIVSFTAHGMEQSVRLYWQVGGQTN
                     NQGFKVYRRRGDGDFQLQDSWLSNPGLSNPTGSTFEWWDTGLQNGVVYTYRISSTNTS
                     EMEFFHNYPVSASAMPIQTLTISNLAGTLTDSVTFGNNPYASDSQDGFWDVTKGNPTE
                     TYVWTAFWQQYWGNQGTSLLREIKGGYDVDSQVKTWVLRTRSDQLEQLSIQATGTFGR
                     AEKLWLQDGATFHNLLSGPYSYSNANSNVRTFNLYWGNMQPRATVASMQNKILQGGNM
                     VSFSWNYTYSFLIDHVELSLQNATDSLLVNNYLGNTQSSYSYLLPQTVDMQGAKLIVD
                     VIAIDGVRTRFVSPYTLALVPYMNLAMLESGWQMLSNPWVTTPLSVDAVFGAGTDAYT
                     YNPVSAWQPTTQFNFGSAVWAHLNDFVFYSSTNPLLADEYNLDLMDGWNLIGNPHLCS
                     YDVENLRFMVNGSLYRFGEMVNQELLSPVVWAFRAGQYEPVSRIEPFQAFLIRYYGAA
                     GLVSQINFYPFWQAPEIDAPSAQSSVTLHAAVNGLSDWVRVGTHNLANDTSDFRLNLA
                     KPPVPPFSAPRLYVPELLPTLRPDGQLHSQFLPLLDVNGASQDVIAFNVRLEVPTADP
                     VTFNWGNNGLPDGWQAAFLIGDTVYHMGSAPEFVWQPDQAGTYNANIRLSNYAVANQD
                     QVASLLGKPLAWPNPFNPDVNIAFSLAAPAQLNAEVYNLRGQKIHALHSGLMNKGNHS
                     LKWNGKDCNGRSVASGIYFVKLSSGKHSQVIKVMLMK"
BASE COUNT         6287 a         7309 c         7255 g         6428 t
ORIGIN      
        1 gattggctgt tgccctcaca gagtgcttgc ttacccatgg gtcgcttggc gcggctatcc
       61 agatcgaagg cacccccaac ttccttgggc ttgggttcgg cgaagtacaa agcgccgtag
      121 tcgtaagcgt caggcaagag cttgccgctg cagtggaaaa gattgcggga caatactcta
      181 tcccgctgct gcgtctggga gaagtgactg aagacgccac cctgaagatc ggcagctaca
      241 tcgatctgcc ccttgccggg ctgcgccagg cctgggaaga tggcctgaaa ctaaattgat
      301 taaaccttat ttaacttgtt acagtgcaac ttatagcgcg tcacaaacta cactcaataa
      361 actaaggagc aggagatgaa aatgggatta ttctttggga accttttctg ggggatctta
      421 ttggtgcttt ggggtatctc gctggtattg aaaagctttg gcgtatcttt gccgcttgcc
      481 aggatctttt tcgcagcgat tatcatcctc tttggggtca gactgctgat ggggggatcc
      541 tggaaggcaa ggtccagcgg gatcaacact cgcagcgtca aaggcgataa aatcgagtac
      601 accactctgt ttgcctccca aaacattgat ctaaaacaaa tcagaccagg tcacaaaccc
      661 atcgaagtca ccgtcgtttt cggttccgcg tatgtggaat tgcctgccga cgtggcgttt
      721 gaggtggagc cgaccacggt gtttggttct ttgatcactc cccgccaatc gcatttcgga
      781 ttcagtgacg acagagaaat cttcaacgcg gattctggag gagagaaagt cttcatcgag
      841 gccaacgcca tttttggccg gctggagttt gtggtgaaac ctgtcgaccg tccccagcca
      901 gcagctcagc cagacagcac aggcagcggt atcggcatct gatcatgcgt cacaccttcc
      961 tcagaagcgg gatgacattc atcatcctgc tactgggtat tacgtggctg ggggccaatc
     1021 aagcggtggg ctatgccttc agcggtggag gagcgcgtgg ttatgcccac attggaatcc
     1081 tcaaagtgct ggaggagaat gggatccgtc ctgattacat aaccggcaca agcatggggg
     1141 cgattgtggg agcgctctac gcgatcggtt acaacgccgc tcagatcgaa gaattgatgc
     1201 tgaggctgaa ttgggattcg ctgttggatg acgtatatcc ccgcaagcag ctctatatcg
     1261 gtcaaaaacg ctgggcccca tacggcgcgg tcaacctgga gatcggaaac aactggcagc
     1321 cccgactgcc ttccagcgtc tgggtgggga acggcctcaa tcttgaactg gccaggattt
     1381 ttgccccagc gggtgataac atggatttca cagccctgcc gattccctat gcctgtgtgg
     1441 ctacagacct cctgagcggg gagccggtga ttttttcgcg gggaaacctc atgcaggctg
     1501 tcagagcctc actttcgatt cccagcctga ttcagccctt tgaactggac ggccatatct
     1561 acatagacgg cggagtgtct caaaacttgc cgatcaaaga agtgatcggc ctgggtgccg
     1621 attatgtggt gggagtaaag accaattcag gcctgagaag cagaaatgag ctcggaaatc
     1681 tgatccaggt gctggaccag accatcaata tcggcatgac ccgcaacctc aacgccggcc
     1741 tggaggattg cgacttgctg ctggaacctg aactggaggg atatcactct actgattaca
     1801 atctggcggc tcaaatcatc gccgccggag aggcttatgc cagagataac ctcgatagga
     1861 tcgtagcttt cagacagcaa gtggaggcgc aagggcccaa aatctggagg ctggcaggtc
     1921 cgcttcccag tccgggcacc aagactctga ccagcatcag ttgcaccggg aatgaccatc
     1981 taagccttgc aaagataaga gattacatgg gaatcacgat cggcggggac tacagcgcag
     2041 accaattggt cgacgcctgc cgggatgcct ggcattcaca gatgtttcac accatttatc
     2101 ctgtgatcgc tacggaagga gagacctata aactcagact gtttgtgaaa gaaaggaccc
     2161 gccgccaact gggtatccaa ctcagctaca gctccgaaga aggtcctctg gccggtggaa
     2221 tacttaagct caacaatatc ctgctaaaaa actccatcct ccaggcggct ttgactatgg
     2281 gcggtaaaac cgaagtcaac ctggattatg tgaaaaactt cggtgaactg tggggatctt
     2341 atttcagggt atatagttac ttctcagaaa accgccgtta ttattatgac gagagcttta
     2401 acaaagttgc cagcgtcaag gctgccgaac tgggcctcag cggcgggttg ggcatctttg
     2461 ccaataaact ggctgtggcg gaggcattta tctatggatt ccgaacccgc ctctatagag
     2521 atatatccac cgtggcgggg atcgattcgc tctatctgat ctccggcgcg ggagtgaagc
     2581 tgtatcatga aagccttgat gattattact ttccatccag tggattcagg ctatctctta
     2641 agtttaactt tgcaagatca tcccaattaa gcgattacat ttattcaaaa gtacactgca
     2701 atgcagctct ttacactccc ttcaatccct ggctgagcct gaaaaccgct ttggacctcg
     2761 gttcatggtt tggtaagcaa caaatcaata tcgatccttt ctatctggga ggttcgaatg
     2821 gatatttggg ctatcagaaa tatgagatca gcgcgccggt gtataaaacc ttcgatgttg
     2881 ggctgaccgc cgagccgcaa cgcaacatca ttaccagttt gggagttcag ggcctcagca
     2941 tcggggatgt ggatgattgg agcaccgacc agaagctgga aggaagcctc tatctggctg
     3001 taggttacag aactgccctg ggaccgctgc gcttctctgt ggccttcagg gaaaagagcc
     3061 atcccaacta ctatctgaac ctgggttacg atctggacgt gtttcacttt tcccggcgtt
     3121 agactctggg ccatggctac acgtcgataa ctttgggatt gacattttgg ccgccgctac
     3181 agattggtcg cttaaaaaag aatctcaaca ggagaaacag gatgcgtgga atatccctcg
     3241 ctttgatgat catggtttta ggtttgggta tactatttgc ccaggcaccg gatatgagtg
     3301 ctttgatagc tactctcaag ttggcgcaga gcggagacgc ggaagcgcag tggcagatgg
     3361 ccggttatta cgagcagggc ctgggagtag aaccagatca gcaggaagct atccggtggt
     3421 accgtcaggc cgccacgagt ggtcatgtcg aagcgcaata caaggtgggg gcgtttttct
     3481 ccagcaacac cgaagaagcg gtgcagtggt atgaaaaggc tgctttacag gggcatctgg
     3541 aagctcagat aaaattgggc aactattaca acaccaccaa tggacacaag aaaaacccag
     3601 cggaagcctt caaatggtat tctctggccg cccgtcaggg tcaccctgag gctctctatg
     3661 aggtaggcaa cgcctattat tatggagaag gggtcgccaa gagccataaa gaggccttca
     3721 atcattataa aaaagcctcg gacggtggcc atgccgatgc cgccatttca ttgggagact
     3781 gttacttcaa cggagaagga acgcagccgg actatgccca ggctttcaaa ctttatgaat
     3841 cccaagccaa aaagaacaac ccccacgccc tctacagcct gggcttttgc tacgaaagcg
     3901 gcaaaggcgc caggatgaac attatccgcg ccaatgccat gtatcagctg gcagctcact
     3961 attgcacgtc tggaaacaag agcacctttg agagttacgc tcaaaagacc tctgcccggc
     4021 tttccaaagt cgatcaggaa aaggccgata agatcagaga cgaatggctg aacaagcagt
     4081 gaagacccgt cccgacaaag cagagattgc agccctttta tcgctcaagt cacccgatga
     4141 gctcaaagcg ctgtttgccc gtgcctatga agtcaaaaaa cgctatgtcg gcaccagagt
     4201 ctggtttcgt ggtctgatcg agctcagtaa catctgttcc aaagactgct tctactgcgg
     4261 catccgttcc ggaaacgcat ccgtgaccag gtacactatc tctcccgatg aggtggtcaa
     4321 agaagcccga tggtgtttcg agcagggtta cggctcgatc gtgttgcaag ctggtgaacg
     4381 gagtgacgcg gcctggacgg agatcatcac agacctgatc ctcaagatta aagcgctttc
     4441 ggatgggaaa ctggggatca ccctgtcttt gggcgagcag agtgaaggtg tttaccgcca
     4501 atggtttgaa gccggagccc atcgctatct gctgagaata gaggcatcca atcccacgct
     4561 ctacaagcag ttgcaccctg cggatcattc ttatgatgcc agagtggagt gcttaagata
     4621 tttaaagaaa gtgggttatc aggtgggcac cggggtcatg attggcttgc cggggcagag
     4681 cgtggatgat ctggcggatg atctgctgtt cttctatgac atcgatgtcg atatgatcgg
     4741 tatgggaccc tttattcccc acaaagacac tcccctcggc cacctcgtcc ccagctttga
     4801 tccagatgag gctatagaca tgggcctcaa gatgatagcc gcctgcagga tattcctcaa
     4861 agacgtcaac atcgcctcca ccacagccct gcaagccctc aaagacgatg gccgcgagct
     4921 tggtctgctg gcaggggcaa acatcttgat gcccaatatc accgacacca gtttccggga
     4981 caagtaccag ctatatgaag gcaaaccctg catggatgag aacgcttctc tgtgccgcgg
     5041 atgcctggaa cgcagaatcc aggcgatagg ggagaccatc gcctacaacg aatggggcga
     5101 ttctgtccat tttagaacca ggacccagac aatcaatcag gcagattgat ggaatggctt
     5161 ggatttcaag cagcttgcgg gctggattgg tgtccggcct tgcgttggac cccacagacg
     5221 ttttatctaa actaaaagga gtttaatatg ttcccaggtg gaaaaggcat gcaggacctg
     5281 atgaaaaagg cccagaagat gcaacaggaa atggaacgca accagaaaga gatggaaaac
     5341 accatctttg aagccagttc cgggggcggg atggtgaaag tggagatgac cggcaaaggc
     5401 gatctcaaaa gcctcaagat cgatcctcag gcggtggacc ctgatgatgt ggagatgctg
     5461 gaagatctta ttgtggcagc gattaccgag gctcagacca aggtcaatga ggcctccgct
     5521 tccatgatgc aaggtctcac cggaggaatt aaaatccccg gtctattctg atcagatgct
     5581 ccaatctccc cgtcttgacg acctcatcac ggccctgaac agactgcccg gcatcggacg
     5641 caagacagcc cagcggctgg cctggcatct gatcggagga gaaaaaagca gagctctgga
     5701 gctggctaac gccattatct cagccgcgga aagctttgct ccctgcaaag tgtgtaacct
     5761 gctggcagag acggacccat gtcaattctg ttcatcggtc gacagagatg actctcagct
     5821 ttgcctggtg gaaaacaatg ccgatgtggc cattatcgag aacatgcatg aatatcgggg
     5881 ccgttattat atcctggggc atctgctttc acctctggac ggtttcgggc cggaacagct
     5941 gggaattcat aaattgatga aactgatcga cgaccgcgct cccgatgagc tgatcctggc
     6001 gctcaaacct tccgccgaag gggaagccac cattcattac ctcaccgaaa ctctcaaggc
     6061 taaacccatc aggatcacgc gtttatccac cggcctgccc tttggtggcg atctggaata
     6121 cagcagcatt ctcacccttt ccaatgcatg gaaaaggcgt tatcccgtat agacttatgt
     6181 ttaccggcat catcgaagca gtagttccca tcctggcagt gcgggagggc agtggcagac
     6241 gccatatcaa gctctcacgt ccccaagcct ttgatgatct gcgagcgggg gccagcatag
     6301 cctgcgacgg catctgtctc acggtcctgg gctttgatca atccagcttt gaggtggagg
     6361 tcatggccga aacgctgaaa aagaccattg ccgctggctg gagaaacggc actctgatca
     6421 atcttgaacg ggcgctcagg ctgggagaca ggttagacgg ccacttggtg cagggccata
     6481 tcgacagagt tctcactctc agcagcacca gaaatcagtc cgatacgctc tatctatatt
     6541 ttcattatcc ccgggaagac gctgctctcc tggtggcaca aggctctgtg tgcctgaacg
     6601 gagtgagcct cacgatcgcg gagttgaaga cagacagctt tgccgtggcg ctgatttctc
     6661 acacgtggca aaacagcaac ctgcctgcct tgaaaccagg ttcacgtgta aacgtggagt
     6721 atgacgtgct ggggaaatac attctgagaa gctcaaaccc atcaaaactg tctgaggagt
     6781 ggttgcgtga acaaggctac tagacttgct tgcatattgt ttctggcttt tatgatgatg
     6841 agttgcggta gcaagaaaaa ccccaccggt ggaccagttg ataccgtcaa accagaggtg
     6901 ctggctactc tgccgcaaga atttggccag ctgacggaca ggatagagat caccttctcc
     6961 aaagccatgg acaaaagcag cttcacggaa ggcctctact tttatcctcc catcagtgcc
     7021 aggaaaatca gctacagcgg tcgcaccctc agcatccgga tcgaagaaaa gctgaaagac
     7081 gataccaatt actacctcac tttgagcacc cggatcaaag acacgcgcgg caatcccatc
     7141 gctcaaagcc gcagcttcgt gttttccagc ggacggctca gcgatctgcg tcttggcggc
     7201 aaaattgatt atgaagagtc tgccgatcag ggcaaaccgg tgaccataga tgtattttcc
     7261 gcggattctc tactggtgct cagccacacc gccaacgggg ccagttatgg catcgaaacg
     7321 ctcaatcccg ctgtttatct cttgaaggct tatcaggaca aagatgccaa cggacgttat
     7381 gacctgggcc gcgaagcgtg gtttgaaggg agagtggacc tcaagcgctc tgccaatctg
     7441 gacctgatga tgagctataa tgacaccact ctgactgtga tcaaagatgc cagagcgatc
     7501 tcagacagaa aagtggagct caccctgtcc aaggtccctg tctccatagg agcggtatcc
     7561 gtgagggcag gaaacgggat cagactgcca gtgctgattt cggagctgga aggcattcga
     7621 ctgacgctga tcacccaagc gcaggacagc ctggattaca gagtggagat cagcgatcta
     7681 aaagatgtca ggggcaatcg caatcccaaa tcgggtattg gcttcaaggg gtccattatc
     7741 aaggatacgg aagcacctat tgtgctgagt tccacacccc gcaacggtac cagcgtctcc
     7801 aatctgttgc ccacgattac tgtgaacttt agcgagatca ttccactggg aaacatgaag
     7861 gcccgcctgc tggcttcaga tcaaaacaaa gagattccgc ttcagatcct caagagcgac
     7921 tccagaagct acagcttcca gcccagtcaa cccctcagca attatcgcag ccacagcctg
     7981 gtgatcctgg ccgacacgcg tgacagcacc ggaaatccgc ttggtaaaga ccacgtgatc
     8041 agctttttac cgctgctcaa gaccaggtaa gcctcccaga gacatattcc ctttatacat
     8101 aggataagag ggcaagcggt attgagtaag gggctcactc cgacgctacc agatttcccc
     8161 actctggtat gtcagactat cttcacctat acctgacacg cactcctccc atgccaaagc
     8221 ataggagagg caaaggagag gcataggtgg tgcaaaggag gctaacaggc aattaactga
     8281 aatcgaagat attgacatac tccatattgt tggagtctgt tacttccacc tcatcaaatt
     8341 cgtccccgtc gggcaggtag aatatgccgt ccagttcgta atcgccattg atccggaagc
     8401 ccatggccgt acctgtgatc acgctcatgg accacttggg cctgtctatg gctttcacct
     8461 tgaagagcag cggcatttct atgtagtcca tgtagtattt cacgttcatc tcggccggct
     8521 tggccagttc ttccatttcc ccaaactcgt tttccatccg gaagatatcg atcttgatgc
     8581 gggagccttt ttgagcgtag agcagctcgt atcccagggc cagattcggc aggatattca
     8641 ggtccaacca ggctccagca taggctcccg gccgcatggc aagcttgacc ttgaggtcct
     8701 ggggatcggc tttggtgcca taatgcaggg acagcgcaaa accagtcttg gcaccatagc
     8761 tgatatcggt ctgcccaaac atggcaatgg ggaagagggc cagcagggca aagagaatcc
     8821 ataccgcgat cttcacatct cctccagtgc cgcggcaatc cgggaacgga tttgctctgc
     8881 caactccaac ttgtcatgat agatagggtc atcggtgaga atgggttcca gcaggcgaat
     8941 cttgatgcgg gcgcggtgga tatgtccgtc gatctcataa gctctgtagc tgcctttgat
     9001 ggcaaagggc acaatagcgg cgtccgccat ggaagccagt ttcaggctgc caatatgaaa
     9061 aggtcccatg ttcgcaccct tgctgcgcgt tccttccgga aagatgatct gagggaagcc
     9121 ggcattgatc accttggcgc tttctttaaa tgattcgatg gcggagcggg gattggaacg
     9181 atcgataaac acgcaattga tgatccgcat ccactggctg agaatgggta cccgtaacag
     9241 ctcttgcttg gcaatgaagc ccaccggttg tccgctccac cccaaaaaag cggggatgtc
     9301 aaagtagctt tgatggttgc ctacgtaaca gagccttagg gagggggaat cgctgtcaac
     9361 gctgtgttga cgtgtggtgg aggtggggat gttttcgagg ccttcaaagc ttactttact
     9421 gcctgtgctg cgcaccacca cccgtcccca aaagtatctg gcgaagaggg tcattttcag
     9481 ataacgctga ggtggcagca gccattttcc caacagatat accggtagcg cgaacaggca
     9541 taagagcata aagaagatta acagagcgta ccaaagatag gtcttgattt ttgccaacat
     9601 gagaagtcct aaagggttga gagtttgaga agttgagagg agttataggg ttgtatgggc
     9661 tggttaacaa gctcgtaaag agggcaaaac atactcgctg gaactgctgc gatcatctca
     9721 ctttatcctg atattactca gtgcgattca gctatcttgc gcgcggcggc aacatatatc
     9781 atccgtctcc cctaatagct catcgtgacc cattcttcat agacatcgtt cagagaggtc
     9841 acccaggccc tgcgtttgac ggcatgattg atggtcttcc agtagaggcg gcccagcatg
     9901 ggaaaatcca cgggatcgaa actggtgtta tgccagagca gagaaagatg agattgatat
     9961 ctggcggcaa gatcgatcaa gcgatagagc acccggcgtg attgagcagg gctcatattc
    10021 atggccttgg gagatctcag ggtggtgtcc atcacgatca aaggtatctc caaaactctg
    10081 aaagggcggt tttccgcaat gttgaacggg aagaatggaa aggaaattcc agcgcggaaa
    10141 ccaatatttt cccagtaacc caaggtggag tcgaaatgta tgccggcatt ttcgaggata
    10201 gtgaagctct tttgataatc gaaatggaga tagtgggtgc ggaaaccctt ggggtcgaat
    10261 cccagttcac gcagtttatc cagctccgct tgcaggacca cgggatcgaa ggccgattcg
    10321 ggtgagccgt gcaggcctac ttcctgttgc cccagcaggt ctatgatctg ctctttggag
    10381 acgggatctg aaaagtaatt ctggcgctga tcttcaaagt catccctggc cagcagaaac
    10441 caggtggatt tgacccccag gtcggtttcc tgacggatga tgcgtttgac gttccgccag
    10501 ggattgtgca gcaggttttt gtgcagtccg tgccctaaaa tcttccaaag cgcgtttagg
    10561 ggcctgcggg ggatggtcct cagattgtag aagagagtct gaaacttctg gcgggtattc
    10621 cagtagttcc aatagtctat gtcgtgcgag agcgatacgc aaaaatcctt atcctcgctc
    10681 cagtaaatct ccctcacgaa ttcgggaata aactgcccca ctgcccacag gaggatctga
    10741 caatagacgt ccaccacagg aatttcggta aaatcccaac ggtattgcag ggattcgcga
    10801 tagtccaccc gacctttgtg ggccccgtga tagttgagga tatactcgtt ccagcaggtg
    10861 agaaaataga agcccccggc caccaggtct ttgtggatga cgcagtgatc tgtgtccagc
    10921 gagaaaatcg ctcctcccct ggaaaagagg aagggaatat actcgttttt atagaggcaa
    10981 aaatctacca ggtcaagcgg atagagctcc cggcgctcaa aaaagtcggc cgtgccgtcc
    11041 ctgaagttta ttttgatggg ataatcgcgc tcggtgggat agccataata gagatgactg
    11101 ccctcatagt ggtttccata cacaaacttg gggttcagcc tcaggatata gcaataggtc
    11161 cgcaacacga actctgcctt gggaatgtag aggcgcggca gattcaggag gatgatgata
    11221 ttgggatact tttccacttc aggagcctgt gccgccttca atccttctat ttgaggtctt
    11281 acgcggtccg ctgtttctct ttacgggttc ccggggctcg ggttttacgt gcaaaccccc
    11341 gatcagaact gctttgagga cgtctctgac ctgatccacg tagctgatgt ccatgccttc
    11401 caggatatcg gcatcgaaat ctgccatgct ggcctcattt tccgccggta cgatcagttt
    11461 gtgaatgccg gcccgtttgg cggccaggat cttttccttg agcccgccga tggggagcac
    11521 tttcccaagc agtgtcactt caccggtcat ggcgatgtcg tgtttgaccg gcttgcttgt
    11581 gaacaaggag gccaacgccg tcgtcagagt aatcccggcc gaagggccat ctttgggaac
    11641 tgccccggct gggaagtgga tatggatatc ccacttgtca aagctgttgg ttggaatgcc
    11701 ataatcctgc tgatgggatt tgaggtgaga gagcgctatg cgggcggatt ccttcattac
    11761 ctctcccaag agcccggtga gcacgattgc cccctttcca ggcatacggg tagtctcgca
    11821 aaagaggatt tcgccgccgt aactggtcca ggcgaggcca gtcgccactc cgatttccgg
    11881 cttgcgattg gccagctcca ggctatattt acggggtccc agatagctgg caatatcgct
    11941 ggctttgagg ctgagggaca actgctctcc tgaggccact tttctggcta tctttctgaa
    12001 gatagagcca atgcgccgtt gcagattgcg cactccggat tcccggacgt aataacggat
    12061 cagttcctgc agggcggatt tttgcagttt gacgatggaa gagctgaggc cgttgtcttc
    12121 cagttcttta gggatcagat aatgccttgc gatctcgatc ttgtcgtgct ccagatagct
    12181 ggtaaactcg atgatttcca tgcggtcacg caagggagcg gggatggttt ccagcgagtt
    12241 ggccgtggtg ataaacatca cttcggaaag atcaaagggc aggttgagat agttgtctgt
    12301 aaagctgttg ttctgttcgg gatccagcac ttccagcagc gcggaagcgg gatcgccacg
    12361 gaaatcacgg cccagtttat cgatctcgtc cagcataaag acgggattgg cggtaccctg
    12421 gcgtttgatc tcggtgatga tctttccagg catggcgccg atataggtac gtctgtggcc
    12481 tctgatctcg gcttcgtcgt gaatcccgcc cagggacatc ctgataaact tgcggttcag
    12541 ggcccgggcc acggatttgc cgatcgaggt cttgcccgta ccgggaggac ccacgaagca
    12601 gaggatcgga cctttgatgc tgcctttcag ctttttgacc gcgatgtatt caatcacccg
    12661 ttccttgggc ttttccagac cgtagtgatc tttggtaagc actttatcga tcttgacgag
    12721 gtccagccgg tctttgctgt aaactctcca gggcagattg acgatccaat ccagataatt
    12781 gcggatcacg ccatattcgc tcgaggccgg ttgcatggat gccaggcgtt ccagctcttc
    12841 gagggcggtg tctctcactt catccggaag gtcgtttttc tctatcagtt cgcgccattt
    12901 gagaatctct ttgctcacct cgtcgctttc tcccagttcg cggcgaatgg cgtccaactg
    12961 ctctctgaga taatagcgcc gctggtcttc gttcatttcc agctggatat tgctgcggat
    13021 gtggttctcc acccgcatct gtttgataag ctcagccagg cagtcattga gaaacctgta
    13081 tctggccttg aggtcgatga gctccagaat gcgttggcgg tcagcaagtt ggagctcaag
    13141 attgccggcg atgatatccg ctaccctacc ggcttgtttg atgtttccca gtccggagat
    13201 catctcacgg ttgaggagat tgccttcctg ggatatcttt tccagcagtt ccagcgctac
    13261 cgtccgatag gcgtggactt ctgtatcttc tatgttcagc tctgtgacgc tttccacgtc
    13321 tgccatgata aagggctcgc gctgagtaac tttctgcagt ctaatcctgg atgtaccctg
    13381 caagagcagg ctaatcgtgc catcctgatt gcgaagcatt ctgaggatgg tgactgcagt
    13441 gccaatctcg tgcaggccgg catcttcgcc ttcctgatcg aggaaaaagg ccatcgattt
    13501 atcattggac agagcgtgat ctatcaccag cttaccttcg tcgtctgcca ccaccagcgg
    13561 catcagcaaa aagggaaaca tcaccacgtt tcccatgtgc aacactggca gggttctggg
    13621 gatccggctg ttatgatctt ccatctgctc actccttgtg catattgact tctgtttata
    13681 tagataagcc gtttgcaatc aaaaccaaac ggctctgcca caaaagcggg cgggggattt
    13741 ttgacaagct atttctgcgc ctgggtctaa agacgctttt gataacgaga tgagcgtatc
    13801 ccgatcggtt gagagactca ggatcccgca gattacgcgg ataatctaaa gtcaacaggt
    13861 tttttttgtt ggtttcagaa tgttttccat ttcttgatta tcaggttctg cgaaatctgc
    13921 gtatcaatct gcgtaatctg cgggaaattc acctaattcc cagagcagta aagatatcgc
    13981 gtttcagttc atcgattgcg gggttgtcat cggaaaggcc tgcgggaact atatattcct
    14041 cgccaaagcg tcccgggtcg cctttcatca agaccacgcg gtgagagagg atgatggcct
    14101 cagagatatc atgggtcacc aaaatgatac tcagagccag cctggaagcg atttctttca
    14161 accacagctg cagctggcgt cgggtgatgg cgtccaggct ggcaaaaggt tcatccagga
    14221 gcagcacttt ggcttcggac atacaggtgc ggaggagtgc cgtcctctgt ctgagacctc
    14281 cggaaagctg ccaggggaga tgactggcat gatcttggag gccaaagacg ggcagcaact
    14341 catccaaccg tttcctggct tgccgaagat ccttttgggc tactctggca gggagcagag
    14401 cattgtcacg cacactcagc cagggaaaca gcagatcgtc ctgaggcata tagcccagtt
    14461 tcccgcttct ggcggtaata tcctctccca taaaaacaac acgcccctga tcgggtctcg
    14521 taatcccggc cagcagctcc agaaaggtgc tcttcccgca gccgctggcc cctatgatgg
    14581 tgagaaactc gcctccagac agtgccagag acgcgtcttc gaggatcaga cgactgcggc
    14641 cgtttagcaa gaatgatttg ctgagaccgg cagcctcaag gatgtggctc attcaggcag
    14701 aaaacggttt gtgaacatct gatcggcttt catgccggtc atcagcatcc gcttgttaaa
    14761 catccaatcc acaaaccgct gccatacctc cagccgctgt tctccccacc tgggagcttc
    14821 tgcctggtat tgatctttga gataagccat gctcagcttg acctgttcgg cattggcagc
    14881 cagttccggg acggccttga tgagaatatc ggccgcagag gagctattct caatgcagta
    14941 tcggtagcct ttagataccg ctgccataaa ggatttcacc agctccggat cggcctgggc
    15001 ggtcttctcg ttggtgacga tcacgggggt ataatagtca aacaccgggt caaggtcttt
    15061 caaaggtatg tagttcagag cgataccgcg tcgttgagct tccacgccgt cccagcccca
    15121 gaatatccac tcaaaatcgg catcccggcc aatggtggaa aagaagtccg tgaccccttc
    15181 caccaccttt agtttatcaa aggacgcgct gtcggcagtc ataacggagc gcaggatctc
    15241 ggtctcggtg ggccagccgg aactgccgta acgtttgccc tcaaagtgct tgggcctggt
    15301 cagctccgct tctttcagag aggcgaagcc cgaagtgttg tgttgaatca ctgccgccag
    15361 ggagaccagc gggatgttct ctatcctggc gcgaataacg ctttcctgat agctgatccc
    15421 aaactgggcg tgaccggatg cgactatctg ttccgcgctg ttctggcctg gctggatgat
    15481 ctcgacctcg aggccggctt ccttgaaata tcccagttcc tgggccacgt agaggcctgt
    15541 atgattggtg ttgggcgtcc agtccagcac tactttgagc ttgcgggcgg ctttctgagc
    15601 ggcttcccgt ttacatgaca ccaggccccc cagggccaag gccgtaagca acaggatgca
    15661 gagagctttt ttcatcttat atctcctaat acgttatgcg ttatgcgtta agcgttacgc
    15721 gatccatatg ggtacacttg ttgcatatca tatatatctt aatctcatat ctttatgtgg
    15781 aaaacctctc tgttttccct gcagtatttc aatcagttct cactgtatca tataatctta
    15841 cacgcaccga acggctgaag ccagtgatac aaaaccatat tctcatatct cattattttg
    15901 cagtctttca ggcgcgggga tggtaaagcc ggtgcgtctc caccaaaaac cgggtgttga
    15961 cgtgggtgta aatctgagtg gtgttgaggc tggcgtgacc cagcagttcc tggaccactc
    16021 tgagattgac gcccgcctcc aaaaggtggg tggcaaagga atgcctgaag gtgtgaggcg
    16081 taaattcctg tttgatcccg gctttcaggg cggcttcttt gaggatcttc cagaagccct
    16141 gccggctcag ttttccgccc ctgccgttca aaaacagata atcggagcgt ttcatcttca
    16201 gcagcgcggg cctgtgggcg ttcagataaa cgtcaaacag cggctcaagc gaattcacat
    16261 aaggcacata gcgttgctta ttgcctttcc cgtgcaccag tatgatcccc tcgctccggt
    16321 tgagatcgtg gaggctgagg cccagcagct ctgagattct catgccggtg gcgtagagag
    16381 tctccatcat cagacggttg cggagttcca gcgaggtctg gacgggttgg gaatccagga
    16441 aacgcagcat ggtgtccaca tccagaaaat ccggtaaatg ctggcctact ttgatcttgg
    16501 gaactttgtc gaaatccatc acgcggttgg catcattatc ggcaagataa cggaaaaact
    16561 gattgagggc ggcccgtttg cgggccaggg agctcactat caagcccata tcgctgagta
    16621 gggtgagata ttcgatcact tcctgatggt caatccgttc gacaggtttt tgcgcgtagg
    16681 ccagaaagtc tctcagatcg cgttcatagc tcacgacact gttttccgcc atgccttttt
    16741 ccacccgaat gtgaaacaca aagtctctta gatagaccag cgcggctggc aagggagggg
    16801 taaaggcctc cgtgtttctg ctttttcctt tcaatactgg ttgtttctct gaggctgcca
    16861 agtagtgctt ccgggatgtc taaatggttt cagaccaagg gagtaactgt ctgtcagtca
    16921 ccgctttgat agttgggtgt ttccttggtg atgcggatat catgggggtg ggattctttg
    16981 agcccggcac tgctgatctc gataaactcg gcatggactt tgagactctc cagatcgcgg
    17041 gctccgcaat agcccattcc ggaacgcaaa cccccgcata gctgatagat atagtctttg
    17101 agggggccct tgtagggtac catcccttct ataccttcgg ccaccatctt gttctcttcg
    17161 gtggcgtttt ggaaatagcg ttccccactg ccgcgtttca tcgctccgat ggaacccatg
    17221 ccacgatagc tcttaaatct gcggccgttg aagatgataa actcgccggg gctttcatcc
    17281 gtgccggcaa aaagggagcc gatcatgaca gcggaagcgc ccccggccag agctttcacg
    17341 atgtcgccgg aatacttgat tccaccgtct gcgatgatgg ggacattact ctgggccgct
    17401 acttcagcgc agtccatgat ggcggtcaac tgaggcactc caatccccgc tatcactctg
    17461 gtggtgcaga tcgagcctgg accgatcccc actttgactg catccgcgcc ggcgtcgatt
    17521 agaaatctgg tggctttggc agtggcgacg ttgccggcga tcacttccac acccagcttg
    17581 tttttgacct ttttgagggc tttggcaatg ttgatctggt gaccgtgggc ggtgtctatc
    17641 accaggaggt ccacacccat gcggacgagt tcctctgccc gctccagaaa gtctcccgtc
    17701 actccgatcg cggcccccac caggagccgg tttttgctgt cctgcacggc gttgggatag
    17761 tctatccgct tcatgatgtc gcgcactgtg atcatccccg tgagctggtc atcggcaccg
    17821 atcagcagga gcttttcgat ccggtgtttt tgcaggagcg ctttggcgtc ttccagactg
    17881 accccgctgg gagcggtcac cagtctgtct ctgggagtca tcagttctct tactttgcgg
    17941 cggggatctg tctcgaaacg gatgtcgcgg ttggtgagaa tccccaccag acggcctttc
    18001 tccaccactg ggaacccgcc gatcttgtgg cttttcttca gctccagcac ttttagcaga
    18061 gtgtcgtcgg gagacacggt ataaggtttg gaaaccaccc cgctctccgc gcgcttcaca
    18121 tgatgaacct cgttggcctg atcagctatg gaaagattct tatggagaat tccgatcccg
    18181 ccttcccggg caatggcaat ggccaaagcg tgttctgtga cggtatccat cgccgcgctg
    18241 aggatgggca cccgcaattg gacgcggggg gtgatcctgg cgctgaggtc cacctcggat
    18301 ggcagcacct cagagcgctg cggcaccagt aaaacatcat caaaagtata ggacttgcgt
    18361 atttttttca tgttggcaat ctccatcagg ggtcaaaata tctctttgcc gctggctgtc
    18421 aatagtcaag ctgaggcagg gacggctgat aaacaaaaag acccgaagcg aagtccgggt
    18481 ccaatatctg tgggcaggtt aatatcagtc ttgggattcc ggtgtttcag cttcttcctc
    18541 ttccaaatcc tcttcttccg tgccgggaac gtagatgctg acatccacgg agcacttgct
    18601 gaggtcggga taaaagtcat cgacgtattc gcgcacggtg gtctcgatat cattgtgttt
    18661 gtcgatgatt tcctgagaat agccgatttt gatggcgatg gtgttgaaac tgcggttgat
    18721 atccagcttg ggacgctcac catagacgtt gttcagaaat tccggcaagg acttcgcaag
    18781 cttggccacg ccgctcagac gtttgtaggc cttcatgcca aaatgggcgg ccaatgcggt
    18841 cccgccaact aacagaagag ctttcaggat cttcttcatc atctcacctc acattgtttt
    18901 gattatttat agatagattt tatctttccc cagctgcggt tctgcgtgga gagaggttgt
    18961 tggatttgag tgtctccggg ggcgacggga ttgagagaaa actcgctgaa accgaagacc
    19021 acgatgccca cgatgctgga aaactgtgtc ccggcggcgg gggagagggc tttctgtctg
    19081 gaaaaggagc cggtcgtgat ggaacactgc ccgctgccgt ccgtgacggc aaatctgccg
    19141 gctttgcttc tggaactggc tacgctggca ctgaggaccc gggcatatac tccctcgtag
    19201 gcctccgctt catcggccct ggaaagctga ccagtggtca cgatcagggg ttgaggcagg
    19261 ccataattgc ggtccagcat ccgataggaa ctgatatcca ggaggcaggt catgccaaaa
    19321 gattcatgca cccggccgcg cactctgatc ttgtctccct gggaaggacg gttacgcttg
    19381 tccgcaatgt agataccgcg ccaggatccg ctgatgggct cggagataaa atatcccccc
    19441 tgagcataat ccgctccggt gacgatcccc tccagagtca cctctctgcc cacataggga
    19501 gagggatagc tgttgtccac tccgggagtg ttggtatact ggatatcata tatagtaaga
    19561 gcaccagccg cggtggctag gcccatcaga atggtcatgg caaatagtag cttcttcatt
    19621 ttgacctctt tgaagcgata tttgtgaagc gcgctaagat gtcaagcaaa atcgcatctg
    19681 atttttcgca accggcgctg cttagcttcg ctctattcgg attactgagc atgaagcgtt
    19741 ccgaaaccgc ttttttttgg tcgcgatgac ggtcgtagcg aggggacggg cgtgaaaaac
    19801 atgaattgat caaagtgatt gttttattga gtgcaggaag ataaaacccg tgggggtgtt
    19861 ttggagcctg tgatgaaggt ccgtccttat tcatgggccg cttcgccgtg tctggctcgt
    19921 cggactcaat cccaataaag ttgggggtga gtgagacttg atagcggcag gctggagtgg
    19981 aagcaacagg ggagggatgg gatcgaggag gttgagaggg ttgaggaggt ttagacacgc
    20041 gccggatcgg aatccgacgc tacaatatag aaccgggacc ccatcgaaag gtcccggtct
    20101 cgcaggcaaa agatcaggtt gagaaggcag gcggtctaaa ccctctcaac catctaaacc
    20161 ctaatgtgcc taaaactcca tcaccggtgc ggagacgggt gtcagcatca gagcgctttg
    20221 gtcggagtat tgtttccaga gctcatagag atggccatag ctcttttcca gttccagaag
    20281 caagccgttg gtgctcaggc ccggcaggga atcgccacgc atatcgatgc ggatcccttt
    20341 attggagctg gtggcgtcca ccagttcgat atcgccttta atcccggtca gatcgcgcat
    20401 atgtatcagc gcggtttcca tgccgcccag atcgtcgatc aggcccagtt ccagagcttg
    20461 ctttcctgtc cagactctgc cttgagcgta agcgtgcacc tgctcggaat ccagattctt
    20521 acggcccgta tccactttac ccacaaagtc ttcatagacg gattcgataa agccggtcat
    20581 cttctgtttt tcttcctcgg tccatttgcg gtagatagag ggaaaatctg cgttgcggcc
    20641 tttcttgacc gtggaccagt ttactctgac cttgttaaac atcttttcag cgttgaacgc
    20701 gatgccgatc actccgatgg aaccggtcag agtggtggct tcggccacga ttttgtcggc
    20761 cttgcaggcg atgtagtaac cacccgacgc ggccatgccg gccatggaga ccaccaccgg
    20821 ttttttgttt tcggtctggg cgagttccag ttctctcagg atgatatcag aggcctgggc
    20881 cgatccgccc ccgctgtcca ctctgaggat gatgcctttg taagcggggt ttttgcgggc
    20941 ggcgcggata agtttgacgg tgctttcggc cgcgatcatg gtgccgggtt cgcctttgcc
    21001 catcacgata ttgccgctgg cgtaaatcac ggctatctgt ttgggactgg cgatgttcca
    21061 gtcatatttc gcgaagctgg tgagcgagcc tttgcgggcg gtgaaaccat agcggtcttt
    21121 gagtttgtcc tcaagctggt cttgatagat gaggctgtcc accagtccgg cggccatggc
    21181 atcctgggcc aggtagtagg ggccgttgtc gatcacgtct tccagcttgc cgttgatgcg
    21241 gctgcctctg ccgttattga tttggacaat catctgctca tagatggtct gcaggatggc
    21301 gtcatagact tcccgctcgg cttcggtcat ggaggtttcc gagaattggt tgccggcgct
    21361 tttgtatttg tggctgcgga aattgagcac ttccactccc aggctgtcca ggagggtggc
    21421 aaagtaggga ctggtgatcg agaggccatg caggtccacg cttcccatgg gattcagcca
    21481 gatgttgtcg gccacggaag cggcaaagat atagcctccg ttgctgatat tgtcatagta
    21541 gcattccacc tttttaccct tggctttgaa actggcaaag gccctcacca gttcctgctg
    21601 gagggcgaag gagctggaaa aagatggatt gatcaaaagg atgccttcca cgccaggttc
    21661 ccgggcggct ttctccagtt cgttgatcac gctgtctatc ccgcgttggt tggaatcgaa
    21721 aaccctgaac gggccgaagc tgtagctggg gctcttatag ctcacgattt cgcctctgag
    21781 cttcatgtcg taccattttt tgccagacag gcccaggaag ggcttccagt tcaggtcgcc
    21841 ggcgttcaca tatcccagca gcacggggtc cttgccatcg ggtttgaggg ccagggatcc
    21901 cagctccgtc ttgccgaagc gcaggctgaa attgagcaag gcgctcttgg aatccaggtt
    21961 gtagctgccc cccagcttca agccatccac gatctgggtg ctgaacccaa gcacgggact
    22021 ggtgaagtca tattccatca gggcgggatt ggtctcgtcc gctacctttt tatagctgat
    22081 gtcgccgctc agttcaagac ggtaggagtg ttgaggcgca aaccagctaa gcggccggat
    22141 cgcgatgccg gctttgtagg cgggagattg acgatagggg ttatcccaca ccatcgccaa
    22201 agaggcggaa ttgtggggac ggaaagtgag gccgttgcgc caccagcctt cattggtggc
    22261 ggaatttctc cagctgtaac gggagccgaa atagagattg cgcaggaaac cgcgctccga
    22321 aaggggtgaa ccgacggcaa agttgtgata gctcacatcg ttccggttct catacacata
    22381 gctgaagccg tcactgttat aaaaaaccca gtgctgtttg atccagtcgt tgttgtcaaa
    22441 gttctgggcg tatcccaggc cgctgatttg gcctgtaccc agcagagatg gattgccata
    22501 aggggaaaac tgatcgtcca tcgaggcgat cggaaattcc aggctgtccg gatcaacatt
    22561 tacggattgg gccccaaggt ctgtcagcac aaagccgagc gaaatcagaa taaagacgaa
    22621 gcgaagcggg gtcatagtct ctcctatcat atataagttc cctactctca acggcctgat
    22681 atggccatca cataaactcg tcctatttcc gcgatcgcgt cccggctgtc aagacctttt
    22741 tttttcagac ctctccaccc gcgctcgaaa aataagcctt gactaatatg ggtgcttgtc
    22801 ctgaaattgt aatcagtgta aaacatctga aaccccatgg agaagccgat gaaaaaaccg
    22861 actttgatca taattgccct gttgctgctg agcatggcac aagccctggt ggtggaaacc
    22921 gggtccttcc gcgatttcct ctacggtgag gagccagcct gtgcttacga caactggata
    22981 tctcacatcg ccgagggcat cgcctcggca aactacaacc tctacgcccc ctatgaccgc
    23041 cagctcaacg ggtttggaga cttccggatc atcaacgcgc tcgagaccgc caactggaac
    23101 gcgctcatcg gactgatcct caccgaatcc tgggatgaag cagagctgat gatcctggcg
    23161 gcgggattcc cctatcagct tgtccaattt aacgataccg acaccggccg tacctattat
    23221 atgctgcgtg aaattcccga tatggccgag atcgacgaca acaacaccgc cgacccctat
    23281 gatgacgaaa gcggagcttt tatgtatgga tggggtctct atatatacaa tccccagggc
    23341 agccgtcccg tcattatcac cgttccccat cccaccgacg attttgccac tcccgccatg
    23401 ggagccctgg ccctggaagt gtgggatgcc agattcatga tcatcagcgg tgccggcaga
    23461 gaagtgaagt ggaccaatgt ggcccccttc acaaactcca agtcgctctc cgatcccacc
    23521 cgcgtagcca accatccttt caacttcgcc tatgacaaat ttgccgatca gatcagaagc
    23581 cagtatggcc tccgtgagtt ctcggcccag atccattcct acgactggaa tcgccacgag
    23641 gggtatccca acaatcaaat ctccgccgga tatcaaaaac tctgccccaa cctgcccatc
    23701 cgcgatctct cggacctcaa caacgacatg atccaccagg ccccgcatct ggtctttccc
    23761 gccaacaccg tcggaatcca cgctcccgtg tatctgagcg actattacag cgtcaactat
    23821 tccatccacg atttcaactg gagcgacggc gtaaacacat atcccgtaaa cgactatgtg
    23881 gacctgccgg cttactccca aaatcatcag atgctctaca ccctcagcgg ctggaacgac
    23941 tatgactcct atgatccctt ctttcatgtg gagatggacg aactgcccaa ctgctacgag
    24001 cagaccgaaa acaactacca ctggttctac ggatgggaca ccgcccagca gaaatgggac
    24061 tatgccaacc tctttggccg ctatatgcag ttttactctc cctgggtata tcatcttgaa
    24121 gccgtgatgg acgatatgtt ccagatgaac gacggcctca cgcccactcc tcccacagag
    24181 ctgagtgtgc tcaattcctc cctgacccac atcactctgg gatggcagcc ttcctccgcc
    24241 tatgattttg acacctatga ggtcctctac gctcccgaac ccatcggaac ggacaactat
    24301 cagatcttca accgcagcaa caacagcttc ctggcttccc aggatgctca gagcatcagc
    24361 gtcaccggtc tcaacaacgc caacacctat tacttcaaga ttcgcgccaa agacaagaac
    24421 ggcaactact ccgagctcag caacgaagtt cagtctattc ccgcgcctgc caacatcgtg
    24481 agtttcaccg cccacggcat ggaacagagc gtccggctct actggcaggt gggagggcag
    24541 acgaacaatc agggcttcaa ggtctatcgc cgcagaggag acggggattt tcagctgcag
    24601 gatagctggc tcagcaatcc cggcctttca aaccccaccg gcagcacctt tgaatggtgg
    24661 gataccggcc ttcagaacgg agtggtgtac acctaccgga tcagctccac caacacctct
    24721 gagatggagt tctttcacaa ctatcccgtt tccgcttccg ccatgcccat ccagacgctc
    24781 acaatcagca atctggccgg caccctcacg gacagcgtca cctttggcaa caatccttac
    24841 gcttcggact ctcaggacgg cttttgggac gtcaccaagg gcaaccccac cgaaacttat
    24901 gtgtggacgg ctttctggca gcagtattgg ggaaatcagg gcaccagtct gctgagggag
    24961 atcaagggcg gctacgacgt cgattcccag gtcaaaacct gggtcctgcg cacccgcagc
    25021 gatcagcttg aacagctcag catccaggct accggcacct ttggccgggc cgaaaaactc
    25081 tggttgcagg acggggcaac tttccacaac ctgctcagcg gcccctacag ctattcgaac
    25141 gccaattcca acgtccgcac cttcaatctc tactggggca acatgcagcc ccgcgccacg
    25201 gtagccagca tgcaaaataa aatcctgcag ggcgggaaca tggtaagctt cagctggaat
    25261 tatacttatt ccttcctgat cgaccacgtg gagctctccc tccaaaacgc caccgacagc
    25321 cttctggtga ataactacct cggcaacacc caaagctcct acagctacct gctgcctcag
    25381 accgtggata tgcaaggtgc taaactaatc gttgacgtca ttgccatcga cggagtccgc
    25441 acccgtttcg tgtctcccta caccctggct ttggtaccat atatgaacct cgccatgctc
    25501 gaatccggct ggcagatgct ctcaaatccc tgggtgacca ctcccctgag cgtggacgcg
    25561 gtctttggag ccggtaccga tgcctacacc tacaaccccg tcagcgcctg gcagcccacc
    25621 actcagttca atttcggcag cgccgtctgg gcccatctga acgactttgt gttctacagc
    25681 tccaccaacc cgctgctggc ggatgaatac aatctggacc tcatggacgg ctggaatctg
    25741 atcggcaacc cccatctctg ctcctatgac gtggaaaatc tgcgctttat ggtgaacggc
    25801 agtctctacc gctttggcga aatggtcaat caggagctct tgtctcccgt cgtctgggcc
    25861 ttccgcgccg gccaatatga gcccgtcagc aggatcgagc ccttccaggc tttcctgatc
    25921 agatactacg gcgccgccgg gctggtcagc cagatcaatt tctatccctt ctggcaagcg
    25981 cctgagatcg atgctccctc cgctcaaagc tccgtcaccc tccacgccgc cgtcaacggt
    26041 ctcagcgact gggtgagagt gggcacccac aatctggcca atgacacctc cgatttccgg
    26101 ctcaatctgg ccaaaccgcc cgttccgcct ttctccgccc caaggctcta cgtgccggaa
    26161 ctgctgccca ccctcaggcc cgatggacag ctccacagcc agtttctgcc tcttctggac
    26221 gtcaacggcg ccagtcagga cgtcatcgct ttcaatgtgc gcctggaagt tccaaccgcc
    26281 gatcccgtca ctttcaactg ggggaataac ggccttccgg acggatggca ggccgccttc
    26341 ctgatcggcg acacagtcta tcacatgggc agcgcgcctg aattcgtctg gcagccagac
    26401 caagccggca cctacaacgc caatatccgg ctcagcaact acgccgtcgc caatcaggac
    26461 caggtggcgt ccctgctcgg aaagcctctg gcctggccca atcccttcaa tccggatgtc
    26521 aatattgcct tcagccttgc cgctcctgcc caactgaacg ctgaggttta taaccttcgc
    26581 ggccagaaga ttcacgcgct gcattccggc ctcatgaata aaggcaatca ctccctgaaa
    26641 tggaatggaa aagactgcaa cggcaggtcc gtggccagcg gtatctattt tgtgaagctg
    26701 tcctccggca aacacagcca ggtgatcaag gtcatgctga tgaaataagc ctctgaaaca
    26761 gcctctatat gaaagcccgg tgggatgaga atgcctgccg ggctttctca tatcggagtg
    26821 gctcatggaa acggaaacgt cacgtttatc cggatcgggc tcattcctga ccgcgtccac
    26881 cagaaagtgg tatatgctgt ccaggatgta catcatcacg atttggagga gtaccttgat
    26941 gagattgccg ggacgtgagc cgttgttccc aagcgagacg attccgagcg tgggtctggc
    27001 aggcaggcgg tggtgatttg gtctgattgc atttgcaact cctctgataa ttatggttgg
    27061 ctgatcaagg ttcccggttt gggaaactta gccttcattt atatacgggt tacggtgctt
    27121 ttggagcgat ccgggcttgg tctgaatacc ggtcccggaa gcttcgatca ctttcatcgt
    27181 ttatcatcaa ttcatcaatt tcggactagc ggcacccccg gggaccggac gggggagaga
    27241 ttctaccgcg tagggatatt aatatatact aaaataata
//
LOCUS       DAOSFN010000013        26211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1465_length_26211_cov_9.001338, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000013 DAOSFN010000000
VERSION     DAOSFN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 26211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26211
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_26211_cov_9.001338"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="PLO35_01910"
     CDS             complement(<1..937)
                     /locus_tag="PLO35_01910"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOH78446.1"
                     /translation="MLNDLEIARQVQLKNIDEIAAGLGLESQDIDHYGEFKAKIRYST
                     LSKLENNPYGKLILVSAITPTPAGEGKTTVSIGLSQALNQLGRKSIVAIREPSLGPVF
                     GIKGGAAGGGWSQVLPMEDINLHFTGDFHAVTTANNTLAALLDNHIYYDNELNIDPRR
                     LTWKRVLDVNDRMLRKIVIGLGGSKQGFPREDGFDITPASEIMAILCLSNNMDELKER
                     IGKIIVGFTYHGEPVFVHQLGYEGAIAALLKDALKPNIVQTTENTPAFVHGGPFANIA
                     QGANSILATKTALRLSDYVVTEAGFGFDLGAEKFFD"
     gene            complement(1085..3283)
                     /locus_tag="PLO35_01915"
     CDS             complement(1085..3283)
                     /locus_tag="PLO35_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH78447.1"
                     /translation="MRKLFTVIAILLLLSEAICVEDTSARIRALQKLLEYKTDTSTLV
                     EERESLIRAASIIPPRGEYESSEMYQRRVNAAQQKINQIQAEYATKISDYEQLANQRR
                     QQIKNQIDELLMKTQETFNTTLFRFLTYDPDNQVLPMYISEINQTISAQVPLARAEYV
                     KTNATKYSLQATRQLNEQLQWSYNNWFLVGEGERYVVGTHQGRDNYAASKTNYMPPDL
                     KASAVLIEPSGNKILDAEEAGAIEVTIKNEGLGSAYAVDMRVSANMMEGIVFDQSLYF
                     GEIGSGQTATQRIALSAMSDVQDRQQSLTLSFTEMYQFPPDQINLSFKTKAIKMPNLV
                     LADKGISDDGKITPGAISEVRLRIQNTGQGTARNAYLEIRSGDVVHLLDTPVRMELGD
                     LEPGRYKDVDIKFAVAKTATDLPLSIRIAEERERFSTDFIPLDLKLNQRQKTTQDIVV
                     QGNDRETGSVSVSQGLSVDIESNIPKGKAKSDAYAVIIGIENYQNLPPAPHAVRDAEW
                     VKEYFQQSLGIPPTNILTYTNDKATMSQFIKLFEGGYISRLIQQKKADLYVYFSGHGS
                     PPPPNQDKPFLAAWDSDFASIEYTCYPVEKVYNALNELKTSNVTVFLDACFSGISKDS
                     KPLKSGKPVYVSPASEMNIGKITLFTAGEFDQIAHIYNDKKHSVFTYHLLKGIHNRAA
                     DFNKDGKITIAEFKKYLSEEVPKLSMQLENELQNPQIYTADLNRVLIEYK"
     gene            complement(3299..4822)
                     /locus_tag="PLO35_01920"
     CDS             complement(3299..4822)
                     /locus_tag="PLO35_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78448.1"
                     /translation="MMFALLPILLAGQQIICGKISYSDNLYEFSFSTATPEFEPSHRY
                     DVFIFAKVGDEGWFKLKTIGGDFENVQAGKTGHLIIWEPYFQGRQKANYSFSIFAVDR
                     AMLEACTPRYSYEAVEGLGTVQLKSNISNSSFQIGQKKFSKPGPVILPNGKYRIKALY
                     QGFKDQEQIVTVVKDSAVSFEAVFPIGYILLETNEPKGKFTAGNITLRPGKSVAVSPN
                     EYFIKATLPGCEDKTEYVKVGYGATAKVRIDFWDYDNTKYLNRMAGFYRSISNMVNEI
                     RVTRCDSGPESVSPVMKYSLAGITYAITPSRSSNAFKKGRAFMYFGMGIADDLGFLVA
                     EVDEKPVIRFAADWVSLQTGLAQSLVNSNFYWRMDAKASFSTQARIHSSIDDIYGRKL
                     VYVTDFEESRTDGGLERKVSDGMMQWSGEATFSIGIRLFKARYLSFYGGVRFNSAKDG
                     NWYLKSEVEQWQAFNMNNEPNESSYHDLPDQNFLQGTSSLIFGVKFNFRDGPGSPFK"
     gene            5263..6816
                     /locus_tag="PLO35_01925"
     CDS             5263..6816
                     /locus_tag="PLO35_01925"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOH78449.1"
                     /translation="MLQIPKPILKLRSKRDTAMLGGGEKRIEEQHDKGKLTARERINL
                     LVDPDSFEEFDLFVTHRCTNFGMDQYVYSGDGVVTGTATINERVVYVFAQDFTVFGGS
                     LSKTYAEKICKIMDMALKNGCPLIGLNDSGGARIQEGVDALAGYAEIFWRNVMASGVI
                     PQISAILGPCAGGAVYSPAITDFVIMEKDNSYMFVTGPKVVKTVLNETVTTEDLGGAG
                     VHSSKSGVAHFIAAGEEEALMLIKKLLSYLPSNNMEDPPYHPSHDPVNRSCDALDQII
                     PENPNKPYDILDVINEIADEGEFMQVMSNFAQNIVVGFCRMNGHPVGMVANQPKVLAG
                     VLDIDASIKGARFVRFCDAFNIPIIVLEDVPGFLPGTNQEHNGIIRNGAKLLYAFAEA
                     TVPKITVILRKAYGGAYCVMNSRHMRADLVYAWPSAEIAVMGPKGAVEVIFRKEAKAS
                     EDPKKALLEREEEYREKFANPYNAAERGYVDDIIEPARTRFRLIRALEMLANKKDSIP
                     PRKHGNIPL"
     gene            6830..7693
                     /locus_tag="PLO35_01930"
     CDS             6830..7693
                     /locus_tag="PLO35_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOH78450.1"
                     /translation="MSRYLLILLVPLLMVTGLQAQAQESGGLELQSSMAAASDSLVKQ
                     RELEVRSEFGFSETDQLDEVAKLLEIKDLNRWKSYLGLEPANKVLDKMNLRKLGITPY
                     KALLAKQFSIYGFTELTTLAELASLKNIPIKKLRNLVGIASLERAKDGFSLQALELDP
                     ARMDSLATEFDKATLPYGISVTLVGMLIVFTALLVTSIIIGQLMHVNKKPKMEDRHLV
                     IDRSGKLVSGHHQIDPNVVAAAITALHLYETGIQERRRLLLTFKRTPTNQWRASQVLE
                     MPNRELFRSRR"
     gene            7697..8413
                     /locus_tag="PLO35_01935"
     CDS             7697..8413
                     /locus_tag="PLO35_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOH78451.1"
                     /translation="MKTYKLRINGVKFDARVMEYTETQAKININGTDYVILIEEDNPH
                     QVPKLASQDKAVPLAPSFSSGFEAGTGELRAPIPGVIASIKVKEGDKVTKGQTIITLE
                     AMKMESEIAAPVDGVIGKISVKERSPVQEGDLLMIIKGEQVKEKPASRPSRNGNAASA
                     PCPPQVTDNVMRAPLPGLVIELLVKAGDMIDTDSTVLILEAMKMESEIHSTLKGRVSK
                     VHVGKGDSVQEGDPLIEVEA"
     gene            8415..9542
                     /locus_tag="PLO35_01940"
     CDS             8415..9542
                     /locus_tag="PLO35_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOH78452.1"
                     /translation="MQELVQFIQTTGFLNFSWGNVLMIIAGLVFIWLAITKGFEPLLL
                     VPIGFGIVIGNIPGVAEQGLGVMDSGSVLNILYFGVSKGVYPSLIFLGIGAMTDFGTL
                     IANPKLILLGAAAQFGIFATFIGSILLGFNILEAASIGIIGGADGPTSIYLASKLAPH
                     LVGPIALAAYSYMSLVPVIQPPIMKLLTTPKERLIRMKPLRVVSKREKIIFPLVAFVV
                     TALIAPGSVVLLAMLFLGNLLKESGVTERLASSARSVLIDVVTILIGLTVGARTTADA
                     FLTPSSIKIFLLGAASFCVATATGVIFAKIMNLFIKDKINPLIGNAGVSAVPASARVS
                     QMVGQQYDKSNYLIMHAMAPNVAGVVGSAVAAGVLLGILGN"
     gene            complement(10485..11438)
                     /locus_tag="PLO35_01945"
     CDS             complement(10485..11438)
                     /locus_tag="PLO35_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78453.1"
                     /translation="MKPPILCLILLIFLTSLSALEITQEHFGYHPGSVRKLKQGSWNQ
                     ASTDLSEGSGKTWSFAMPSMGFVNNGYSQINGATGFPSANIACHYTQSINGIDDSGTL
                     YYQNNGTDILSLGYTAATDLVWNPPIPTGLPHTPGKTWQGNHTYTYGSYTVTGKVISE
                     GTISTPLGSYQALCVHYHYNTTNFNYDIYQWETEVYGIIAYALSLNGGMLYVLEEATP
                     NVTALEDECLVPIPRLKVYPNPVQDFLKVITPQSLSSQWDISLYDLRGRQVKDFGNHL
                     VPPSESEIRLEINPGELPYGIYILRAANPQQRFHTRIVISD"
     gene            complement(11440..11877)
                     /locus_tag="PLO35_01950"
     CDS             complement(11440..11877)
                     /locus_tag="PLO35_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH78454.1"
                     /translation="MSEPRAKISVKRYAVADETIYAILTDIWARTGVGNPARGDDLAT
                     IQTTLRHGGLIILAYHGDTAIGATWLTHDWRRLYIHHMAVLEEFRDQGVGRMLLNEAL
                     AYASEIGIQPKLEVHHANPRARHLYESAGFTYIEGYWVMVKRG"
     gene            complement(11882..12808)
                     /locus_tag="PLO35_01955"
     CDS             complement(11882..12808)
                     /locus_tag="PLO35_01955"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOH78455.1"
                     /translation="MKHRNLILLAVFLVLLGLFFFLRNREPKEKLRKVIDADSTQVAH
                     IHIYNVADTIVVAKQNGQWMLQYPEQARVNPDMLRVFFEFLRDATYSGTPMTESDNNL
                     ARYGLDASKALQIRVLDNAGKLIAHCHFGNTDNPYDYFRYDKDSKVYQVKQPIWGRLQ
                     PQVDSWRSPTILSIRWDQMDAISVKHANNSYVLTRQGPDWHYADSREQFKIPPGNVTM
                     GKLLNVLANLEAYTYKRPEEINRALLSEVADVKIEMTDKSSHSIKFHLWDKEYLMTVD
                     GDESKNYSMVFDQVQRFTRHAEVFRLKEMEGE"
     gene            complement(12805..14373)
                     /locus_tag="PLO35_01960"
     CDS             complement(12805..14373)
                     /locus_tag="PLO35_01960"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOH78456.1"
                     /translation="MKGSKRDQILSAYLLRIGIAVMVLLVAAYLYFRVDVSHGKAYSL
                     GKYSRETASKLQDRVVVKIYATKDLPGEFSTVHRYLKDVLTEFRRAGKGKFSYEYARF
                     KDAAELREQAQINGLQPIPVQMYENDQIVMKEVILGVVFESQGRFDVMNIYPGLEPTL
                     EYELTKRIQNIDGKLLPSVTVFQDSIYKYFPTQLITDELNDNYNVVVTDLDSLPRFNP
                     VMLFTGVVASLSDTQLYNLDQYIMKGGKVVFLQDRINSDQNSAFTFDSNLFDLLAHYG
                     VLVHPNMVLDRTGDVRSGRGLGQQLPYPIYPIVRGMENSPITRGTDNTVMYFASEIAP
                     LDSVNIKFEPILQTSADSGKLPGPNFNLEAVLFPQGGQYTLGKPPITVAASVKGKLKS
                     YFGDKPQLQKPGFIASRDNAEFTIFGDRELYMDPDKPEFINRCYVVLNAIDHYLENPS
                     MIRIRTRRMFSSRLDIPYYMYKKNIMPAEPLPVMNRIKATFKAIAIALPPLMMLIMGL
                     LVWLSRRPKGVHAS"
     gene            complement(14376..15098)
                     /locus_tag="PLO35_01965"
     CDS             complement(14376..15098)
                     /locus_tag="PLO35_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH78457.1"
                     /translation="MNPAITIAKKEYSLAFRSVTTYIIFILFLTATGFYFANTALKVG
                     LAELRGVFGIIHLIFVIYAPAITMSAISKEQASGTFELLSTLPLKLGQIVWGKIMAAV
                     MLLATVLVFTLVYLGLILHFGSGVDLGAIFAGYLGLLLAGAAYISIGVFASSLSTNQV
                     LAFILGLAISAVFYLMQFITAFLPASLAGTLEFISFDYHLQNFLKGVIDTRDLLFFLI
                     LIVVFKYLAELKLQSRNMMQER"
     gene            complement(15095..16177)
                     /locus_tag="PLO35_01970"
     CDS             complement(15095..16177)
                     /locus_tag="PLO35_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH78458.1"
                     /translation="MIEIKSLSRGFGHIRAVDKISFAVNTGEIVGFLGPNGAGKTTTM
                     RMMVGFLQPEEGSIELDGKSIFADPISTSARIGYLPEHNPLYPEMSVVEFLAYIAALR
                     KMPKGRYLERLDYVIDNCGLEGVLNQRIGTLSKGYKQRVGLAQAILHDPEVLILDEPT
                     SGLDPNQIMEIRELIRELGQAKTVLLSSHIMQEVQALCDRVIIINKGRIVVDELKENL
                     GAYMGNAQHLVLEVEADQPDLSDWSLLYPDTQLQEQTQHGNVARIKMLIPGENDLRRE
                     LSAFVVEKGWQILSIYAEKQSLEEIFHNLTMQEELPDTPEEPQPSSWENDAMTDDGSE
                     SDATEGQQSSSVEEDTLPSHEEEEAK"
     assembly_gap    16214..16313
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16314..17478)
                     /locus_tag="PLO35_01975"
     CDS             complement(<16314..17478)
                     /locus_tag="PLO35_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH78459.1"
                     /translation="MRKKWKIVTGIAIGLVVIVFAIGQCGKAAMKKMTLSQPTQTHTV
                     ARGDIESRIEITGEVQPQTVVSIKSKVSGKIVKFYASENDYVKQGQKIADIEPDYNQA
                     NTLFSTKAQLQRSELRLANAKKDLADKQVLLDKKYISQESFDAASDELRNAQIEFTQA
                     TSQYEMIRELDVPGKVTHVFATASGVVIERKINEGEMVQSSMNSYGEGTVVMKIADLK
                     HMIVKSNINEVDIAKFSLGQSADIKLDALPYEAFEGSIVKIAPMAATENNAKVFPVEI
                     SINASGEKAKPGMTAAVSILGESRKDVLTIPIRAVFSNDNNEDIVYLMPAGAKKSADK
                     QAISKSTAAPKPVATPVKLGANDMQMVEVISGLKEGDQISLSEPDQPPSFPGMM"
     gene            complement(17610..18140)
                     /locus_tag="PLO35_01980"
     CDS             complement(17610..18140)
                     /locus_tag="PLO35_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOH78460.1"
                     /translation="MKKIVLILAVALIAMVSLFAKKATPVATVKNVDLNRYLGKWFQI
                     AYYPNSFQPKDCGLTTAEYSMDARGKIIVKNTCFEDSQGQKIKKRATAKAWSVSKSNA
                     RLKVRFFWPFTGDYWIVRLEENYQWAVVSDPGRKYLWVLARNNTMDKDTWADMANWLI
                     SNGWQPSRLKITGEIR"
     gene            complement(18223..18699)
                     /locus_tag="PLO35_01985"
     CDS             complement(18223..18699)
                     /locus_tag="PLO35_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOH78461.1"
                     /translation="METRPSWQQYFMEMALLAARRSTCLRRQVGAILVKDNQILSSGY
                     NGSPKGIAHCADVGCLREQQNVPSGQNHELCRGVHAEQNAIIQAGLNGSSTRNAVLYC
                     THQPCSICARLIINAEIRTVYVAHPYPDKLAEQLFREAGTEIIHYDPQSGRMSRLI"
     gene            18906..19487
                     /locus_tag="PLO35_01990"
     CDS             18906..19487
                     /locus_tag="PLO35_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOH78462.1"
                     /translation="MSKAHMIVLNFLNRKPMYGYQIGQIIEQHRMPAWSGVKLAAIYK
                     SMQNMERQRYIKGEEQREGNNPPRTVYHITSRGKEHLRQLIIKALGDVQSFPTDWWMA
                     LSLARNSISRPDMLNSVELRLKHLTQVVMKKCKEAEHLNDLVEDERPFVADEALMLGK
                     MWIKDEIKVLKALRQAIQDGKYDAGFYDRGEGK"
     gene            19484..20851
                     /locus_tag="PLO35_01995"
     CDS             19484..20851
                     /locus_tag="PLO35_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH78463.1"
                     /translation="MRQICLTILLLTTLTALFSNPIPTNARVIDLVEAEAMALQNNPE
                     YQSSRASYESASWARTSALSAFLPSLSLGGTWLYMDPATTVSTAGGSITLNNDMRTIS
                     LSLSQPLFVGGKLWNAYKIAGLGKDIANTSLKNAELSLKTGVLAKYLDLLQLKQLYEI
                     GRDDLNSALINQELAQIKLENGLITMADFLRFKSRVASKEVSLLQSTTALQIGLKDFA
                     NYLGAQEPVVPAPLADPEQDELLAALDAMQAPAVEVFVQKASALGTKSNPSLDILKQS
                     LQISKRGASIAKGSFLPTVMLTGSRQYKENGIDRYEFSTADQIMLNVSIPLLPQIGNY
                     AAWKKAEWEYRKALYDSQNAEQGIVLGIEAAVLNLVSSAKQVRAAKLTLEYTQVTYEQ
                     LQERFKLNMLSTLELLDAELMLSSTRMAYTNSLYSFYKNRGTLAGLLGTADPQVLNNL
                     IINAE"
     gene            20854..21930
                     /locus_tag="PLO35_02000"
     CDS             20854..21930
                     /locus_tag="PLO35_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH78464.1"
                     /translation="MKPRYIVISVIMVLLLALSACGKKAKAPTDKQDERPVVIVEELV
                     PRDLDEYVSVSGKLEGITDITMSSEASGRILQLYKKLGDRVAKGERIGQVENDALRLR
                     LEQAEAALVSAQSSFDNAQKNLSYAETAWQRKLISQAEYNSAMAAFKGAKAGLDGAKA
                     GVESASQAVRGSYLSAPEAGVISNLLVAAGQYIAPGTPVASITNASRLILKTGVGESQ
                     ISKIKQGQAVELSYPGMEQSFKGQVRGFGIRPVQSGNTYPIEIETSGGGKLLPGMVVS
                     ARILSTRYKDLLYTPITNVVNEFGKNYVYIVDAATRAQKMEVTLGRVIGENVEILSGL
                     QAGDKVVTSGADNLESGTPVQIRG"
     gene            21940..25110
                     /locus_tag="PLO35_02005"
     CDS             21940..25110
                     /locus_tag="PLO35_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOH78465.1"
                     /translation="MSVSETALKYSILVNMLTLGILIFGLISMVNMPREEFPAVDFGQ
                     TLVIVVYPGVSPAEMEQLVMRKLEVELADLDDLDYITGSAEEGRVMLRVVFDTSVSSE
                     TAFDRVSRELNKVTDLPDDAMDPIVIRLNMREVNPIAQVVVGGDKYDALALRDISEDL
                     KDGLLTVSNISKAELVGARERQVWVDVDQARLDAYGLTLSDLSAALQGRNLNIPGGTS
                     KFGKIEFLVRTLGEFDSIEELGSMIVQSDAGGRAIRLDEVSAVRDTLQKPETIARLNG
                     SESISIFLYKKGDGNIISVMKEVRQYIDNFKRGFPGLDVSVRNDGSIDVRNGINALGT
                     NAIIGILLVFLALFFFLGWRNALLASFGIPLSLLITFIVVPLFDVTLNNLTIFGMIIV
                     VGMVVDNAIVVLENIHRLREEGYCHKDSITLGVNQVISPVFSSTLTTISAFMPLLLLG
                     GVMGAFLGVFPIVVSVALLGSWFQSMVVLPNNVYQFGRGLPTGQDRTTRLIAPFVERY
                     KQILTKVLKHRQIAIWSSVGMLLLSFLVLGSGAIRFEFFPSSQSQTIQLQMQTPVGTT
                     LAETEAVVSQVEDFIMNMPQKEDVDFVVGNVGAIGGEGMREVATNNAQVNIDLVDQDE
                     MKYTHDDIRGAIRKFMDKLPGVYSYKFGAGQAGPPVGSDVDLRVVGDDLERLTYISEE
                     IKAVLKGIPGVVDIDDSFGKGKKEVKIIPNQTKLSMNGLSVAQIASTVRMASTGMEVS
                     KFRGSGVDEYPLIVKLQDRYTEELENLKNLKIRTRMGEMIAIRDLAEFEIATSLSRIE
                     HRDQRRELSVTAAAGTYNKGGKLVKRSASEINSLLFGGGISREQGLLSDFDQRFPGYT
                     ITAGGVQEEQRKSYSSLGWLFLLALLLIFTILASQFRSYVQPLIVMMTIPFAFIGVIL
                     GLLVTGLPFSLNTMISVVALAGVVVNNAIILIDFINQEREKGADRWHAILNSGAVRLR
                     PILLTTATTVAGMLPLVFSGDPSAQAWRPLAVSFTFGLVFSSVITLFIIPVIYSMVDS
                     FFGRFKMTRFTEHLKFTEAIECKTDE"
     gene            25355..25879
                     /locus_tag="PLO35_02010"
     CDS             25355..25879
                     /locus_tag="PLO35_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78466.1"
                     /translation="MAYPAPDSDPCGAFCDHYKPRRKLEAKIRQNLAQGEIPRGMGYF
                     YPKTFSYSAPILGLMIGAYVFNAFASYLGVSEIQALGILIPVMALMLFLVFRGRRVIL
                     VTDHRLLFIGPGRKGLPRDVAIQSVNNAAMKGSTLKLEIDGRKESWVVDNYSSLAWKK
                     SRRQEFIEAIKQIG"
BASE COUNT         5921 a         6812 c         7167 g         6211 t
ORIGIN      
        1 gatcaaagaa tttctctgct cccaggtcga aaccgaaacc cgcttccgtg accacataat
       61 cgctgagccg cagggctgtc ttggtggcca gaatgctgtt ggcaccctgg gcgatattgg
      121 caaaagggcc tccatgcaca aaggcggggg tgttttccgt ggtctgaacg atgttgggct
      181 taagggcgtc ctttagtaga gcggcgatcg ctccctcata accaagctgg tggacaaaca
      241 ccggctcgcc gtgataggtg aagcccacaa tgatcttgcc gatgcgttct ttgagctcgt
      301 ccatgttgtt ggaaagacag aggatagcca taatctcgct ggcgggggtg atgtcaaatc
      361 catcctcgcg ggggaagcct tgtttgctgc ctcccaagcc gatcacgatc ttgcggagca
      421 tgcggtcgtt gacgtccagc acccgtttcc aggtgagccg gcggggatcg atgttgagct
      481 cgttgtcata atagatatgg ttgtccaaaa gcgcggccag ggtgttgttg gcagtcgtca
      541 cggcatgaaa atccccggtg aaatggaggt tgatatcttc catgggcagc acctgactcc
      601 atcccccacc cgcagcgccg cctttgatgc caaacaccgg tcccagagag ggttcgcgga
      661 ttgcgactat ggactttctg cccagttgat tgagcgcttg cgacaagcct atcgacacag
      721 tggttttgcc ctctccggcc ggagtgggtg tgatggctga aaccaggatc aacttgccat
      781 agggattgtt ttccagcttg gaaagcgtgg aataacggat cttggctttg aattctccat
      841 aatgatcaat atcttgagat tccaggccca ggccggccgc gatctcgtct atgttcttga
      901 gctgcacctg tctggcgatt tccagatcgt tgagcatgct tacctccagc ttgaataatt
      961 cgcctgccgt caagcagact tatagatagt ctaatgcttt gaatggcttt ggcaatcggc
     1021 accaatcagg gacctgagat atggctcgcg aagccagatc tttttaccac tcacggaccg
     1081 gctgctattt gtactctatc agcactctgt tcagatcggc ggtatagatc tgtgggtttt
     1141 gaagctcgtt ctccagttgc atggaaagct tgggtacttc ctcgctcaga tacttcttaa
     1201 actctgctat ggtgattttg ccatctttat tgaagtcggc tgcccggttg tgaatcccct
     1261 tcaggaggtg ataggtgaac acactgtgtt tcttgtcgtt ataaatatgg gctatctggt
     1321 caaattctcc agcggtgaag agagtgatct ttccgatatt catttcgctg gctggtgaga
     1381 catagaccgg ctttccggat ttgaggggct tgctgtcttt gctgatgccg gaaaagcagg
     1441 catccaggaa gactgtcaca ttgctggtct taagttcgtt gagggcgtta tagacttttt
     1501 cgaccggata acaggtgtat tctatcgacg cgaaatccga atcccaggcg gccagaaacg
     1561 gcttgtcctg attgggagga gggggtgaac catgcccgga gaaatagaca tagaggtcag
     1621 cttttttctg ttggatcagc ctggaaatgt agcctccctc aaagagtttg ataaactggg
     1681 acatggtggc tttgtcgttt gtataggtca ggatattggt gggcgggatg ccaagtgact
     1741 gctgaaaata ctctttgacc cattccgcgt cccggaccgc gtgtggggcg gggggcagat
     1801 tctgatagtt ttcgatcccg atgatcaccg cgtaggcatc ggatttggct ttgccctttg
     1861 ggatgttgct ttcaatgtcg actgaaagcc cctgcgacac actcaccgag ccggtctcgc
     1921 gatcgttgcc ctgcaccaca atatcctgag tggtcttctg cctctgattg agcttgaggt
     1981 ccaacgggat gaaatcggtg ctgaaccgct cccgctcttc cgcgattctg atcgacaacg
     2041 gcaagtctgt ggcggttttg gcgaccgcga acttgatatc tacatctttg taacgtcccg
     2101 gttccagatc accaagctcc atccggactg gcgtatcaag caaatggacc acatcgccag
     2161 acctgatttc caggtaggca tttctggcag ttccctgccc agtgttttga atcctcaatc
     2221 tcacttctga aatggcaccg ggagtgattt tgccgtcatc gctgatccct ttgtccgcca
     2281 gaacaagatt gggcatcttg atcgccttgg ttttgaagga cagattgatc tggtcagggg
     2341 ggaattgata catttcggtg aagctgagag tgaggctctg ttgcctgtcc tggacgtctg
     2401 acatagcact cagcgcgatc ctctgggttg cggtctgtcc actgccaatc tctccaaaat
     2461 agaggctctg atcaaagacg atgccttcca tcatgttggc cgaaactctc atatcgacag
     2521 cgtaggcgct ccccagtcct tcgtttttga tcgtgacttc gatcgctccg gcttcttcgg
     2581 catcgaggat tttgttgccc gaaggctcta tcagcacagc gcttgccttc aggtccgggg
     2641 gcatgtagtt ggtcttggaa gcagcgtaat tatcccttcc ctggtgtgtc cccaccacgt
     2701 agcgttcacc ctcgcccacc aaaaaccagt tgttgtaaga ccattgcagc tgctcattca
     2761 actggcgggt ggcctgcaga gagtactttg tggcgttggt ctttacatat tccgctcggg
     2821 ccagagggac ctgagcgctg atcgtctggt tgatttcgct gatgtacatg ggcagcacct
     2881 ggttatcggg atcataggtc agaaagcgaa acaatgtggt gttaaaagtc tcctgggtct
     2941 tcatcagcag ctcgtctatc tggtttttga tctgctgtct tctttggttg gccagttgct
     3001 cgtagtcact gattttggtc gcgtattcgg cttgaatctg gttgatcttt tgctgggcgg
     3061 cgttcaccct tctttgatac atttcactgg attcatactc gccccggggg ggtattatac
     3121 tggctgcccg gatcagactt tcacgttcct cgaccagggt ggaagtgtct gttttatatt
     3181 ccagaagctt ttgtaaagcc ctgattctgg ctgatgtgtc ttccacgcag atcgcctcgc
     3241 ttagcagcaa gagtatcgcg attacagtaa acagcttacg catgagacct cctagtcatc
     3301 atttgaaggg cgaccccggt ccgtcccgga agttgaattt tacaccaaag atcagcgaac
     3361 tggtcccctg taggaaattt tggtctggca ggtcgtgata actactctca ttgggttcgt
     3421 tgttcatatt gaaggcttgc cattgctcaa cctcagactt cagataccag tttccgtctt
     3481 tggccgagtt gaatctgacc cctccataaa aagataagta gcgggcctta aacagcctga
     3541 tgccaatgga aaaagtagcc tcgccagacc attgcatcat accgtccgaa acttttctct
     3601 ctagtccacc atctgtacgg gactcttcaa aatcggtgac atagacaagc ttgcgaccgt
     3661 agatgtcatc aatggaggag tgaatcctgg cttgagtgct aaaggaggct ttggcatcca
     3721 tccgccaata gaaattgctg tttaccagcg attgggcaag gccggtctgg agagataccc
     3781 aatccgccgc aaacctgatg acgggtttct catccacttc ggctaccaaa aagccgagat
     3841 cgtcggcaat tcccatccca aagtacataa aagcgcgccc cttcttgaag gcattggaac
     3901 tcctggaagg ggtgattgcg tacgtgatgc cggccagaga gtatttcatc acggggctca
     3961 cgctttccgg gcctgaatcg catcttgtta ccctgatttc gtttaccatg ttggagatag
     4021 aacgataaaa acctgccatc cgattcagat acttggtgtt gtcataatcc caaaaatcta
     4081 ttctcacttt ggccgttgcg ccgtatccca cttttacata ctcagtttta tcctcgcagc
     4141 ccggcagggt ggccttgatg aaatactcat tgggtgacac cgccacggat ttgccaggcc
     4201 ttaaggttat gttgccggcg gtgaattttc ctttgggctc gttggtttcc aagagaatat
     4261 atccgatggg aaacaccgcc tcgaaagaca cggccgagtc cttaactacg gttacgatct
     4321 gctcctggtc tttaaaaccc tgatacagag ctttgatcct gtatttgcca ttggggagga
     4381 tcactggacc tggcttgctg aatttcttct ggccgatctg gaagctggag ttgctgatgt
     4441 tggatttcag ctgcactgtt cccaggccct ctacagcctc atatgagtat ctgggagtac
     4501 aggcttcaag catggcgcgg tcgacagcga agatcgagaa gctgtaattg gccttctgtc
     4561 tcccctgaaa gtatggttcc cagatgatga gatgacctgt cttgccagcc tgcacattct
     4621 caaaatcccc tccgatcgtt ttcagcttga accatccctc gtcgcccact ttggcaaaga
     4681 taaacacgtc gtagcggtgg ctgggttcga actccggagt agccgtcgaa aagctaaact
     4741 catacaggtt gtcgctatag gatatctttc cacagatgat ttgctgcccg gccagcagga
     4801 tgggtaagag agcgaacatc atcaggaata gtgctttaca tatgtggtta gccttgctgg
     4861 ccagtctcgg cgcggttctg aaatcgtccc cggcatggct gacggcggac tggaaaccgg
     4921 gctttagaaa gactgaaggg gggggtgagg ctcttcgtgg agtcgttttc gccactccaa
     4981 aagatgataa ccgagccgaa caggacttgt cgcacaaaag aaagcattga tcgtttagca
     5041 agtgcctaac ctctttatac cgctatatat ccacttgcgg actaaataac tggcctatcg
     5101 ctttcccgtc aagaattatt tcaaatccca aaacttgacg aaatctcctt atcactttca
     5161 tcacaaggat gatggcataa tctcgttaaa aaagctttac gaaaaaagcc cagtataaat
     5221 aatggcctcc aaatggccag acaattgaaa tgagaggacg agatgctgca gataccgaag
     5281 ccgatcctga aattgcgctc caaacgggac accgccatgc tgggcggtgg cgaaaagcgc
     5341 atcgaggaac agcacgataa gggcaaactg actgcccggg agcgtataaa cctgttggtg
     5401 gatcccgaca gctttgagga gttcgatctc ttcgtgaccc accgctgcac caactttggg
     5461 atggaccaat acgtctattc cggagatggc gtcgtcaccg gtaccgccac catcaatgag
     5521 cgggtggtgt atgtcttcgc tcaggatttc accgtctttg gcggctcgct ttccaaaacc
     5581 tacgcggaaa agatctgcaa aatcatggac atggccctca aaaacggttg tccgctcatt
     5641 ggcctcaacg atagcggtgg ggccagaatt caggagggag tggacgccct ggcaggctac
     5701 gcggagatct tttggcgcaa cgtgatggcc agcggagtga tcccgcagat ctcagccatc
     5761 ctgggcccct gcgcgggtgg agcggtctat tctcctgcca ttacagactt tgtgatcatg
     5821 gaaaaggata acagctacat gtttgtgacg ggccccaagg tggtgaagac ggtgctcaac
     5881 gaaactgtga ccaccgaaga cctcggtggt gccggcgtgc attccagtaa gagcggtgtg
     5941 gcccatttca tcgctgccgg agaggaagag gccctcatgc tgatcaagaa actgctcagt
     6001 tatctgccct ccaacaacat ggaagatcct ccctatcacc ccagccacga tcccgtcaat
     6061 cgcagttgtg acgctctgga ccagatcata ccggaaaatc ccaacaaacc ctatgatatt
     6121 ctggacgtga tcaacgaaat tgccgacgaa ggcgaattta tgcaggtgat gagcaacttt
     6181 gcccagaaca tcgtagtggg attttgcagg atgaacggtc atccggtggg catggtggcc
     6241 aatcagccca aagtgctggc aggcgtcctg gacatcgacg cttctatcaa aggagcgcgc
     6301 tttgtgaggt tctgcgacgc tttcaacatc cctatcatcg tgctggaaga cgttccggga
     6361 ttcctgcccg gcaccaatca ggagcacaac ggcatcatcc gcaacggtgc caagctgctc
     6421 tacgccttcg cggaagccac cgtccccaag atcaccgtga tcctcagaaa agcttatggc
     6481 ggtgcctatt gcgtgatgaa cagccgccac atgagagccg atcttgtgta tgcctggcct
     6541 tccgcggaaa tagctgtgat gggtcccaaa ggcgcggtag aagtgatctt ccgcaaggaa
     6601 gccaaggcat cagaagaccc caaaaaggcc ctcctcgaac gtgaagagga ataccgcgag
     6661 aaatttgcca atccttataa cgccgctgaa cgtggctatg tggatgacat catcgagccg
     6721 gcccgcacca gatttcgtct gatcagggcc ctggagatgc tggccaataa aaaggacagc
     6781 attccgcctc gcaaacatgg caacatcccg ctctaatacg agatctcaga tgtcacgata
     6841 tttacttatt cttctagtgc ctctgctgat ggtgactggc ttgcaggccc aagcccagga
     6901 atccggaggt ctggaacttc aaagcagcat ggccgccgcg tcggacagtc tggtaaaaca
     6961 gcgcgaactg gaagtgcggt ccgaattcgg tttcagcgag acggaccagc tggacgaagt
     7021 ggcaaagcta ttggaaatta aggacctgaa tcgctggaag agctatttgg gcttggaacc
     7081 ggccaacaag gtcctggata aaatgaatct gcgcaagctg gggatcactc cttataaagc
     7141 tctgctggcc aagcaattca gtatatacgg attcacggag ctcaccaccc tggcggagct
     7201 ggcgtccctc aaaaacatcc ccatcaaaaa gctgcgcaac ctggtcggaa ttgcttcttt
     7261 ggagcgtgct aaggatggct tttcgctcca ggccctggaa ctggatcccg cccgtatgga
     7321 cagcctggca acggagtttg acaaagccac cctgccctac ggtatatccg tgaccctggt
     7381 ggggatgctg attgtgttta ccgccctgct ggttaccagt atcatcatcg gccagctgat
     7441 gcatgtgaac aaaaagccca aaatggaaga ccgccatctg gtgatcgacc gctccggaaa
     7501 gctcgtttcg gggcatcatc aaatcgatcc caacgtagta gccgccgcca tcacagctct
     7561 gcacctttac gaaaccggta ttcaggagcg ccgcaggctc ctgctcactt ttaaacgcac
     7621 tcccaccaat caatggcggg ccagccaggt gctggaaatg cccaaccgtg aactattccg
     7681 ctccaggaga tagccaatga agacctataa actcaggata aacggcgtga agtttgacgc
     7741 ccgggtgatg gaatataccg agacccaggc caagatcaat atcaacggca ccgattatgt
     7801 gattttaata gaagaagata atccccacca ggtccccaaa ctggcctcac aggacaaggc
     7861 cgtgccgctg gcaccaagtt tttccagtgg attcgaagcc ggaaccggag agctcagagc
     7921 ccccatccct ggcgtgatcg cctctatcaa agtaaaagag ggagacaagg tcacaaaggg
     7981 gcagacgatc atcaccctgg aagcgatgaa gatggaaagc gagatcgcgg cccccgtcga
     8041 tggagtgatc ggcaagatca gcgtcaaaga gcgttctccc gttcaggaag gcgaccttct
     8101 gatgatcatc aaaggcgaac aggtgaagga aaaacccgca tcccgtccgt cccgaaacgg
     8161 caatgctgct tccgcgccct gtcctcctca agtcactgac aatgttatgc gggccccttt
     8221 acctggtctg gtgatagaac tacttgtcaa ggcgggagat atgatcgaca cagacagcac
     8281 cgttttgatc ctcgaagcca tgaagatgga atccgagatc cacagcaccc tcaaaggccg
     8341 cgtctccaaa gtgcacgtgg gcaaaggcga cagcgttcag gaaggcgacc cgctgatcga
     8401 ggtggaggct taaggtgcag gaactcgttc agtttatcca aaccaccggc tttctaaact
     8461 tcagctgggg caacgtcttg atgataatcg ccggtctggt gttcatctgg ctggcgatca
     8521 ccaaaggttt cgaaccgctt ttgttggtgc cgattggttt tggcatcgtg atcggcaaca
     8581 taccaggagt ggcggaacag ggcctcggag tgatggattc aggcagtgtg ctcaacatcc
     8641 tctattttgg cgtcagcaaa ggcgtctatc cctccctgat atttttggga atcggagcga
     8701 tgacggactt tggaaccctg atcgccaatc ccaagttgat tctcttgggc gcggcagctc
     8761 agtttggcat ctttgccacc tttataggtt ctatcctctt ggggttcaat atcctggagg
     8821 ccgcctccat cggcattatc ggaggcgcgg atggccccac atccatctat ctggcttcca
     8881 aactggcacc ccacctggtg ggtcccatcg ctctggcggc ctattcctac atgtctttgg
     8941 tgcctgtgat ccaaccgccc atcatgaaac tccttaccac cccaaaggag cgcctcatca
     9001 ggatgaagcc cctgcgggta gtgtccaaac gcgaaaaaat catctttccc ctggtggcat
     9061 ttgtggtcac agccctgatc gcgcctggct cagtggtgtt gttggcgatg ttgtttttgg
     9121 gtaatctgct caaagaaagc ggagttacgg aacgcctggc cagctcagcc cgcagcgttc
     9181 tgatcgacgt ggttaccatc ctgattgggc tcacggtggg ggcccgcacc actgcagatg
     9241 cctttctcac acccagttcc atcaagattt tcctgttggg ggctgcctct ttctgcgttg
     9301 ccaccgccac gggagtgatc tttgccaaga tcatgaacct ctttatcaaa gataagatca
     9361 atcccctgat cgggaatgcc ggagtgtctg cagtcccagc ttcggcaagg gtttctcaga
     9421 tggttggcca gcaatacgat aagtcaaact atctgatcat gcatgccatg gcgcccaacg
     9481 tggcgggtgt ggtaggttca gccgtcgcgg caggggtgct gttgggaatt ctgggcaact
     9541 gacagagccg cgggatttct caaatggagc ggtaaacgtc ctcgcttgct tgtaggtatc
     9601 gtcggattcc aagccggcga gtgtttaaac tatagagcta cagaggattt cttttttcac
     9661 tacagagcta cggaggaaac agagagagtt acagtgatga gttttttagg atttgtcaat
     9721 tctgtaacgt cgcattccag tatgtcgctt gcccgcaacg cttaacgcgt aacgcttcac
     9781 gatataacac agagaaaagc aaaggcatct atgtttatgg aacagcaaag gtccccgatt
     9841 gctgaggcgc gattggcgcc tgtggaatgg gctgcaggct ttacaacaat cgcttgtctc
     9901 cacaaaaaac aatccacccc accgaaacac ggacgtttcg ggttccacaa gcaagcctat
     9961 ggcaacgatg cgtttccggc tgcaaaggca tagtccatat tgctttggcc tccatccctt
    10021 ccgatatgtc tccatagaga taccgtgccg ccgatgggga ttttcggcgg agagcgcacg
    10081 gcatctcaag ggcggctaaa gagcagggat gcggtgagat agattaatca tgtcaaatcg
    10141 gttccggacc cgctcttact tatggaccgc gtgcctgtcc agatcatgcc ttacccatcc
    10201 cctatctgct tcgggatggg taggggatgg tccagagggc caggagggat acgaataagg
    10261 gagaaccctc caccggtctg gagatcaatg tccatggtca gattatgcga ttaattcttt
    10321 gctttatatg gctttgacct tcaagctgta catggaaaac agatatctca ccggtgggct
    10381 ggttgcagtc cactgggccc gcaaaattgc tgccatatat agacttatcc tggccgcatg
    10441 aactccaaag gggtgatggt aatccgcagc cgtcagctct cagttcagtc gctgatcacg
    10501 attcgggtat ggaatctctg ctgcggattg gcggccctga ggatgtagat cccgtaagga
    10561 agctcgccgg gattgatctc aagcctgatt tccgattcgg aaggaggcac gagatgattg
    10621 ccgaagtctt tcacttgcct gccgcgtaga tcatagaggc tgatatccca ttgggaagaa
    10681 agactttgcg gagtgataac cttcagaaaa tcctggacgg gattgggata gactttgaga
    10741 cgcgggatgg ggaccaggca ttcatcttcc agggcggtga cgttcggagt ggcctcttcc
    10801 aggacgtaaa gcattccacc gttgaggctt agagcatagg cgatgatgcc atagacttcc
    10861 gtttcccact gataaatgtc gtagttgaaa ttggtagtgt tgtaatgata atgaacacat
    10921 agggcctggt aactaccgag gggagtggag attgtgcctt cagaaatcac tttaccggtt
    10981 accgtgtagc tgccataggt gtaggtatga ttgccctgcc aggtttttcc aggcgtgtga
    11041 ggcagaccgg tggggatggg aggattccac accagatcgg tggcagcggt gtagcccaga
    11101 ctgaggatat cggtgccgtt gttttgatag tagagggtgc cgctgtcgtc gatgccgttg
    11161 atggattgcg tgtagtggca agcgatgttg gccgagggaa atccagtcgc gccgttgatc
    11221 tggctgtagc cgttgttcac aaaacccatg ctgggcatgg caaagctcca ggtttttcca
    11281 ctaccttcgc tgagatcggt ggaagcttga ttccaggagc cctgcttgag ctttctgacg
    11341 ctgccggggt ggtagccaaa atgctcctgg gtgatctcca gggcggaaag tgaggtaaga
    11401 aagatcaaca ggataagaca gagaataggg ggtttcatgt cagcctcttt tcaccatcac
    11461 ccaataaccc tcgatatatg taaatcccgc tgattcatag agatgcctgg cgcggggatt
    11521 ggcgtgatgc acttccagct tgggctggat gccgatttcc gaagcgtagg ccagtgcttc
    11581 attgaggagc atacgtccca caccctggtc tctaaactct tccaggacag ccatatggtg
    11641 gatgtagaga cgacgccagt cgtgggtgag ccaggtggcg ccgatggctg tatctccatg
    11701 ataggcaagg attatcagcc ctccatggcg cagcgtggtc tggatggttg cgaggtcatc
    11761 accccgggcg ggatttccca ctcccgtgcg agcccaaata tctgtcagga tggcatatat
    11821 ggtctcatcc gcaaccgcgt agcgtttgac gctgatctta gctcggggct cgctcatggc
    11881 attattcccc ttccatttct ttgagacgga aaacctccgc gtgacgggtg aagcgctgca
    11941 cctgatcgaa gaccatgctg taatttttgc tctcatcgcc atccaccgtc atcagatatt
    12001 ccttgtccca cagatggaac ttgatgctgt gagagctttt atccgtcatt tcgatcttga
    12061 cgtccgccac ttcactcaga agggcacggt tgatctcctc aggccgtttg taggtatagg
    12121 cttccagatt ggcgagcacg ttgagcagct tgcccatcgt gacatttccg ggcgggattt
    12181 tgaactgctc ccggctgtct gcgtaatgcc agtcaggtcc ctggcgggta agcacatagc
    12241 tgttgttggc gtgctttacg gagatcgcgt ccatctggtc ccagcgaatg ctgaggatgg
    12301 tgggagaacg ccacgaatca acttggggct gcagacgtcc ccagatgggc tgtttgacct
    12361 gatagacctt gctgtcttta tcatagcgga aatagtcgta aggattgtcg gtattgccaa
    12421 agtgacagtg ggcgatgagc ttgccggcat tgtccaacac tctgatctgc aaggctttgc
    12481 tggcgtcgag gccatagcgg gccagattgt tgtccgattc cgtcatggga gtgccggaat
    12541 aggtggcgtc tcttagaaat tcaaagaaaa ccctcagcat atcaggattc accctggcct
    12601 gttcaggata ctggagcatc cactggccgt tctgtttagc cacgacgatg gtatccgcca
    12661 cgttatagat atggatgtgg gccacttggg tggaatcggc atctatcact ttgcgcagct
    12721 tttctttagg ctcgcggtta cggaggaaaa agaagaggcc cagcagcacc aggaagacgg
    12781 ctaaaaggat gagattgcgg tgtttcatga ggcatggact cctttgggcc ttctgctgag
    12841 ccagaccagc agtcccataa tcagcatcat caatggcggt aaggcgatgg cgatggcctt
    12901 gaatgtggct ttgatgcggt tcatcacagg taaaggttca gccggcatga tgtttttctt
    12961 atacatataa tatgggatgt ccaaacggct ggaaaacatc ctgcgagtgc ggatgcggat
    13021 catggaggga ttctccagat agtggtcgat ggcattcagg accacatagc agcggttgat
    13081 gaattccggt ttgtcggggt ccatatagag ctcgcggtct ccaaagatgg tgaattcggc
    13141 attgtcacgc gaggcgatga atcccggttt ctggagctgt ggtttatccc cgaaatagct
    13201 tttcagcttg cctttgacgc tggccgccac ggtgatggga ggcttgccga gggtgtattg
    13261 tccaccctga ggaaagagca cggcctcgag gttgaaattg gggcctggaa gcttgcctga
    13321 atctgccgag gtctgcaaaa tcggctcaaa cttgatgttg acgctatcca ggggagcgat
    13381 ttccgaggcg aaatacatca cggtgttgtc tgtgcctctg gtgatggggc tgttttccat
    13441 tcccctcacg atggggtaga tgggataggg aagctgctgg ccaaggcctc gcccgctgcg
    13501 gacgtctccg gtcctgtcca aaaccatgtt gggatgcacc agcacgccat agtgggccag
    13561 gaggtcgaag agattggaat caaaggtgaa ggcgctgttc tggtcgctgt tgatcctgtc
    13621 ctgcaaaaac actaccttgc cgcctttcat gatatactgg tccaggttgt agagctgagt
    13681 gtcggaaagg gaggcaacca ctccggtgaa gagcatcacg ggattgaagc gcggcaggga
    13741 atccagatcg gtgaccacga cgttgtagtt gtcgttgagt tcatctgtga tcaactgagt
    13801 ggggaagtat ttgtagatgg aatcctgaaa gacggtcacg ctgggcagga gcttgccgtc
    13861 gatgttttgg attctcttgg tgagttcata ttcgagggtg ggttccagac cgggatagat
    13921 gttcatcacg tcaaagcggc cctggctttc gaagacgacg cccaggatca cttccttcat
    13981 cacgatctga tcgttttcat acatctggac gggaatgggc tgcaggccat tgatctgagc
    14041 ctgttcacgc aattccgcgg catccttgaa acgggcatat tcatagctga atttgccctt
    14101 gccggcacgg cggaactccg tcaggacgtc tttcagatag cggtgcacgg tgctgaactc
    14161 accgggtaag tctttggtgg cgtatatttt gaccaccacc cggtcctgga gcttggaagc
    14221 ggtctcgcgg ctgtatttac ccagtgaata agcttttcca tgggaaacat cgacgcggaa
    14281 atacagatag gctgccacga gcaacaccat cacggcaatt ccgatcctga ggaggtatgc
    14341 gctgaggatt tggtctcttt tgctgccttt catctctacc tctcctgcat catattgcgg
    14401 gactgtagct tgagctcagc cagatatttg aagaccacga tcaggatcag gaagaacagc
    14461 aggtcccggg tatcgatcac gcccttgagg aagttttgca gatggtagtc aaagctgata
    14521 aactccaggg tgccggccag gctggcaggc aggaaagcgg tgatgaactg catcagataa
    14581 aacaccgcgc tgattgccag accaaggatg aaagcgagca cctgattggt ggaaaggctg
    14641 gaggcgaaga cgccgatgct gatgtaggct gctccagcca gcaacaatcc caggtagccc
    14701 gcgaagatgg caccgagatc gactccggag ccgaaatgga ggatcaagcc caggtaaacc
    14761 agcgtgaaga ccagcaccgt ggccagcagc ataacagctg ccatgatctt gccccacacg
    14821 atctggccaa gcttcagcgg cagggtggaa agcagctcaa aggtgccgga agcctgctct
    14881 ttggagatgg cactcatcgt gatggcgggc gcgtagatca caaagatgag gtgaatgatg
    14941 ccaaacacgc ctctcaattc agccagtccc accttcagag cggtgttggc aaagtagaaa
    15001 ccggtggcgg tgagaaagag aatgaagatg atgtaggtgg tcacggagcg gaacgcaagt
    15061 gagtattctt tcttggcgat ggtgatggcg gggttcattt ggcctcctct tcttcatggc
    15121 tgggaagtgt atcttcctca acggaagagg attgctgccc ctcggtagca tcggattccg
    15181 atccgtcgtc tgtcattgca tcgttttccc atgatgacgg ttgcggctct tctggtgtgt
    15241 ccggcagctc ttcctgcatt gtgaggttgt ggaagatctc ttccaggctc tgcttttcag
    15301 cgtagatgct gaggatttgc cagccttttt ccaccacgaa ggcggaaagc tctctgcgga
    15361 ggtcgttttc gccggggatc agcattttta tcctggcgac attgccgtgt tgggtctgct
    15421 cctgtaactg ggtatcggga tagagcaaag accaatcgct cagatcaggc tgatcggctt
    15481 ccacttccag cacgaggtgt tgggcgttgc ccatgtaggc acccaggttt tccttgagct
    15541 catccaccac gatgcggcct ttgttgatga tgatcacgcg gtcgcagagg gcctgcactt
    15601 cctgcatgat gtggctggaa agcaagacgg ttttggcttg tcccaattcg cggatcagct
    15661 ctctgatctc catgatctgg ttgggatcga gtccactggt gggctcatcg aggatcagga
    15721 cttcgggatc atgaaggatg gcttgcgcca ggcccacccg ctgtttgtaa cccttggaga
    15781 gagtcccgat gcgctgattg agcactcctt ccagaccgca gttgtcgatc acatagtcca
    15841 gcctctcaag atagcggcct ttgggcatct ttctgagggc ggcgatgtag gccagaaact
    15901 ccaccacgct catttcaggg tagaggggat tatgctccgg aaggtatccg atccgggcgc
    15961 tggtggaaat gggatcggca aaaatactct ttccgtccaa ctcgatggat ccctcctcgg
    16021 gctgcagaaa gcccaccatc attctcatag tggtggtttt tccggctccg ttgggaccca
    16081 aaaagccgac aatttcgccc gtgttgacgg cgaaactgat cttgtccacg gcccggatgt
    16141 gtccaaagcc gcggctcagc gattttatct caatcatcta tactccatga aagttctagg
    16201 gttttagggt tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacatcat
    16321 tcccggaaag ctgggtggct ggtccggttc ggaaagcgag atttggtccc cttctttgag
    16381 acctgagatc acttccacca tctgcatgtc gttggccccc agtttgacgg gtgttgcaac
    16441 cggttttggc gccgcggtgc ttttggatat ggcttgcttg tccgcagatt tttttgctcc
    16501 tgccggcatc agatagacga tatcttcgtt gttgtcgttg ctgaagacgg cccggatcgg
    16561 gatagtgagt acgtccttcc tggattctcc caggatggaa acggctgccg tcatgcccgg
    16621 tttggccttt tctccgctgg cattgatact gatttcgacg gggaagacct tggcattgtt
    16681 ttcggtggcg gccatgggag cgattttaac gatgctgccc tcaaaggcct catatgggag
    16741 ggcatccagt ttgatgtcgg cactttggcc cagactgaat ttggcgatgt ccacctcgtt
    16801 gatattgctt ttcacgatca tgtgtttgag gtcggcaatc ttcatcacca cagtgccttc
    16861 gccatagctg ttcatgctgg actgcaccat ttcgccctcg ttgatcttgc gctcgatcac
    16921 cactccggaa gcggtggcaa acacgtgagt tactttgccc gggacgtcca gttcgcggat
    16981 catttcatat tgggaagtgg cctgtgtaaa ctcgatctga gcatttctga gctcatcgct
    17041 ggcggcatcg aagctttcct gggagatata cttcttgtcc aggagaacct gtttgtccgc
    17101 caggtctttt ttggcgttgg cgaggcggag ctccgacctc tgcaactgag ctttggtgct
    17161 gaagagggtg ttggcctgat tgtagtccgg ctcgatatca gcaatcttct gtccctgttt
    17221 cacatagtcg ttttcagagg cgtaaaactt cacgatcttg ccgctcacct tgcttttgat
    17281 cgaaaccacc gtctgaggct ggacctcacc ggttatctcg atgcgggatt cgatgtcgcc
    17341 tctggcaaca gtgtgagtct gagtcggttg ggacagtgtc atttttttca tggctgcctt
    17401 gccgcactgg cctatggcaa acacgatcac gacgaggcct atcgcgattc ctgtcacgat
    17461 cttccatttc ttacgcattg atcctcgctt gtgccaaata tttccagctt ttgcgaacat
    17521 cgcgctggaa gggcgtgatg ctgtcaataa aaaaagaccc cgggtgataa gcctgcactg
    17581 gaaggtctgt taaagccggg agcgggagtt tatctgatct cacccgtgat tttaagcctg
    17641 gatggctgcc agccgttgga aatcagccag ttggccatat cggcccaggt atctttgtcc
    17701 atggtgttgt ttcttgccag gacccaaagg tattttctgc ctggatcgct caccacggcc
    17761 cactgataat tctcctccag cctcacgatc caataatcgc ctgtgaaggg ccaaaaaaag
    17821 cgcaccttca gtctggcgtt gcttttgctc acgctccagg cctttgccgt ggcgcgtttt
    17881 ttgatcttct ggccttgcga atcttcaaaa caagtgttct tgacgatgat cttgcctcgg
    17941 gcgtccatgc tgtactccgc ggtggtgaga ccgcagtctt tgggctggaa gctattgggg
    18001 tagtaggcga tttgaaacca tttgcccagg tagcggttga ggtccacgtt ttttacggtg
    18061 gcgacggggg tggccttttt ggcaaacagg ctcaccatgg cgatcagggc cacggcaaga
    18121 atgagcacta tctttttcat tttaactcct gaaaaaagct cgttgggatg taaaactatc
    18181 agagtgtgac atgggaagtc caagctctca gccctgtggc gttcatatca gacgactcat
    18241 ccttccgctt tgcggatcat aatggataat ttcagtgccc gcttccctga aaagctgttc
    18301 ggcaagttta tccggataag gatgggccac atagacggtt cggatttcgg cgttgatgat
    18361 cagacgggcg caaatgctgc agggctgatg ggtgcaatag agcaccgcgt ttctggtgct
    18421 ggagccattg agaccagcct ggatgatggc gttttgctcc gcgtgcacac cccggcagag
    18481 ttcatggttt tgtccggagg gcacattctg ttgttcccgc aggcaaccca catcggcgca
    18541 atgagctata cctttaggac tgccgttgta gccgctggag agtatctggt tgtctttcac
    18601 gaggatagcg cctacctggc ggcgcagaca agtgctgcgt ctggcggcca gaagggccat
    18661 ttccatgaag tattgctgcc aactggggcg tgtttccatt gattctccca aatctttatg
    18721 aggtcataaa agcagggggg tgatatctgt caagcggggg ctcgagagga tttgtatctg
    18781 cttgcatcgc gagcagatgc gatcgtgtag agaaaaagga cttgccagat atggcaatta
    18841 tgattacgtt gactatgatc cagtataata gtggagtaaa caataatatc gcataggagc
    18901 ttacaatgtc caaagcgcat atgatcgtgc tgaactttct gaaccgtaaa cccatgtacg
    18961 gctatcagat cggtcagatt attgagcagc atcgcatgcc ggcctggtcc ggcgtgaagc
    19021 tggcagccat ctacaaatcc atgcaaaaca tggaaaggca gcgctatata aaaggtgaag
    19081 aacagcgcga gggcaacaac cctccccgca ctgtctatca catcacgtcc cggggaaagg
    19141 aacacctgcg ccagcttatc atcaaggccc tcggtgatgt ccagagcttt cccaccgact
    19201 ggtggatggc gctctcgctg gccaggaaca gcatctcccg gccagacatg ctcaacagcg
    19261 tggagctcag gctgaagcac ctcacccagg ttgtgatgaa gaaatgcaag gaagcggaac
    19321 atctcaatga cctcgtggag gatgagcggc cctttgtggc agatgaagct ctgatgctcg
    19381 ggaaaatgtg gattaaagac gaaatcaagg tgctgaaggc actccggcag gcgatccagg
    19441 atggcaaata cgatgccggc ttttatgatc ggggggaagg caaatgagac agatctgctt
    19501 gacgatcctg ctcctgacga cgcttacagc gctgttttca aaccccattc ccacaaatgc
    19561 cagagtgata gacctcgtgg aagcggaagc catggccctg caaaacaatc ctgaatacca
    19621 atcctcccgg gcctcctatg aatccgccag ctgggccagg acctcagcac tatccgcttt
    19681 tctgcccagc ctcagccttg gcggcacctg gctctatatg gaccccgcca ccacggtaag
    19741 cacagcgggg ggcagcatca cgctcaacaa cgatatgcgc accatcagcc tcagcctttc
    19801 ccagcctctg tttgtgggcg gcaagctctg gaatgcctac aagatagccg ggctgggaaa
    19861 agacattgcc aacaccagcc tcaaaaacgc ggaactcagc ctcaaaaccg gcgtgctggc
    19921 caagtatctg gacctgctgc agctgaaaca gctctacgaa atcggcagag acgacctcaa
    19981 cagcgctctg atcaaccagg aactagccca gatcaagctg gaaaacggac tcatcaccat
    20041 ggccgacttt ctgagattca agagtcgcgt cgcctccaag gaagtgagct tgctgcagag
    20101 tacgacggcg ctgcagatcg ggctcaagga ctttgccaac tatctgggag cccaggaacc
    20161 cgtggtccca gcccctctgg cagatccgga gcaggatgag ctgctggcag ccctcgacgc
    20221 catgcaggcc cctgccgtgg aagtgtttgt gcaaaaggcc tccgcgctgg gaactaaaag
    20281 caaccccagt ctggacatcc tcaagcaaag cctgcagatt tccaaacgcg gcgccagtat
    20341 cgccaagggc tcctttctgc ccaccgtgat gctcactggc agcaggcaat acaaggaaaa
    20401 cggtatcgac cgctacgagt tttccaccgc ggaccagatc atgctgaacg tctccattcc
    20461 cctgctgcct cagataggaa actacgctgc ctggaagaaa gcggaatggg aatacaggaa
    20521 agccctctac gacagccaaa acgcggagca gggcatcgtc ctggggatcg aagctgcggt
    20581 cttgaatctg gtgagcagcg ccaaacaggt gagggccgcc aagctcaccc tggaatacac
    20641 ccaggttact tatgagcaac tgcaggaacg ctttaagctc aatatgctct ccaccctgga
    20701 gctgctggac gcggaactga tgctctcgtc cacccgcatg gcttacacga acagcctcta
    20761 cagcttttat aaaaaccgtg gaactctggc cggtctgttg ggcacggccg atccacaagt
    20821 gctcaataat cttatcatca acgcggagtg aagatgaaac ccagatatat agtcattagc
    20881 gtcataatgg tcctgctgct ggccctgagt gcctgcggaa aaaaagccaa agcccccaca
    20941 gacaaacaag atgagcgtcc cgtcgtgatc gtggaagagc tggtgccgag agacctggat
    21001 gagtacgtga gcgtctccgg caagctggaa ggcatcaccg atatcaccat gagcagcgag
    21061 gccagcggcc gcatcctgca gctttacaag aaattgggtg acagagtggc caaaggcgaa
    21121 cgtatcggtc aggtggaaaa cgacgccctg agactgcgtc tggagcaggc ggaagcggct
    21181 ttggtatcag ctcagtccag ttttgacaac gctcagaaga acctcagcta tgccgaaacg
    21241 gcctggcagc gcaagctgat ttcccaggcg gaatacaaca gcgctatggc agccttcaaa
    21301 ggtgccaaag caggactgga cggcgccaaa gcgggggtgg aatctgcctc ccaggctgtg
    21361 agaggatctt atctcagcgc tccggaagcg ggggtgattt ccaatctgct ggtggcagcg
    21421 ggccaataca tcgctcccgg taccccagta gcatcgatca ccaatgccag ccggcttatt
    21481 ctcaaaaccg gggtgggaga atcccagatt tccaagatca aacaaggcca ggcggtggaa
    21541 ctcagctatc ccgggatgga acagagcttc aaaggccagg tgagggggtt tggcatccgg
    21601 ccggtccaga gcggcaacac ctatcccatc gagattgaga catcgggagg gggcaaactg
    21661 ctgccgggca tggtggtcag tgcccgcatc ctcagcaccc gctacaagga tctgctctac
    21721 acccccatca ccaacgttgt caacgagttt ggcaaaaact atgtctacat cgttgatgcc
    21781 gccacccggg cccagaaaat ggaagtcacg ctgggacgcg tgatcggcga gaatgtggag
    21841 atcctcagcg gactgcaggc aggcgacaag gtggtgacca gcggagcgga caacctggaa
    21901 tccggaactc ccgtgcagat cagaggatag gtaaacataa tgtctgtatc cgaaactgcc
    21961 ctgaaatatt ccatcctggt caacatgctc actctgggca tcctgatctt tggattgatc
    22021 tcgatggtaa atatgccccg ggaagaattt cccgcggtgg attttggcca gaccctggtg
    22081 attgtggtct atcccggtgt ttcacctgcc gagatggagc agctggtgat gcgcaaactg
    22141 gaagtggaac tggccgacct cgacgacctc gattacatca ccggtagcgc tgaagaaggc
    22201 agagtgatgc tgagagtcgt cttcgacacc agtgtgagct cagagacggc tttcgaccgc
    22261 gtcagccgtg aattgaacaa agtcaccgat ctcccggacg atgccatgga ccccatcgtg
    22321 atcaggctca acatgcgtga agtaaatccc atcgcccaag tcgtggtcgg gggagacaag
    22381 tacgacgctc tggccctcag agacatctcc gaagacctca aagacggcct gctcaccgtg
    22441 agcaacattt ccaaagccga gctggtgggc gcgcgggagc gccaggtctg ggtggatgta
    22501 gatcaagcca gactggatgc ctacggcctc acgctttcag atctctcagc cgcactgcag
    22561 ggacgcaatc tcaatatccc cggcggcacc agcaagtttg gcaagatcga atttctggtg
    22621 cgcaccctgg gcgagtttga ttcgatcgaa gagctgggct cgatgatcgt gcagtccgat
    22681 gccggtggaa gggccatccg tctggatgaa gtatccgccg tgagagatac gctccaaaag
    22741 ccggaaacca ttgccaggct caacggcagt gaatcgatca gcatcttcct ctataaaaag
    22801 ggagacggca acatcatcag cgtcatgaaa gaagtccgcc agtatatcga caacttcaag
    22861 cgcggctttc cgggcctgga cgtgagcgtc cgcaacgacg gctccataga tgtccgtaac
    22921 gggatcaacg cactgggcac caatgccatt atcggtattc tgctggtatt tctggccctc
    22981 ttcttcttcc tgggctggag aaacgcgctg ctggcctcat ttggcattcc gctcagtctg
    23041 ctgatcacct ttatcgtagt gccgctcttt gacgtcaccc tcaacaacct caccatcttc
    23101 ggcatgatca tcgtggtggg aatggtggtg gacaacgcta tcgtggtcct ggaaaacatt
    23161 catcggctca gagaagaggg atactgccac aaagattcca tcaccctggg agtcaatcag
    23221 gtgatctcgc cggtgttctc ttccacgctt accactatat cggcctttat gcctctgctg
    23281 ctgctgggag gcgtgatggg agcgtttctg ggcgtgtttc ccatcgtggt ctccgtggcc
    23341 ctgctgggat catggtttca gagcatggtg gtgctgccca acaacgtgta tcagtttgga
    23401 cgtggtctgc ccaccggtca ggatcgcacc acccggttga tcgctccgtt tgtggagaga
    23461 tacaagcaaa tcctcaccaa ggtgctgaaa catcgtcaga tagccatctg gagctcggtg
    23521 gggatgctgc tgctctcttt cctggtcctg ggttccgggg cgattcgctt tgaattcttc
    23581 ccctccagcc agtctcagac catccaactg cagatgcaga ctcctgtagg caccaccctc
    23641 gccgagacgg aagccgtcgt ctctcaggtg gaagatttta tcatgaacat gccgcagaaa
    23701 gaggacgtcg attttgtggt gggcaacgtg ggtgccatcg gtggagaggg catgcgggaa
    23761 gtggccacca acaacgctca ggtcaacatc gatctggtgg accaagatga gatgaaatac
    23821 actcacgacg atatccgcgg cgcgatccgc aagttcatgg acaagctgcc cggcgtgtat
    23881 tcctacaaat tcggggccgg ccaggccggt cctcctgtcg gcagcgatgt ggatctccgt
    23941 gtcgtgggcg acgatctgga acgtctcacc tatatctcgg aggagatcaa agccgtcctt
    24001 aaaggcatcc ctggcgtggt ggacatcgac gacagcttcg gcaaaggcaa aaaagaggtg
    24061 aagatcatcc cgaaccagac caagctctct atgaacgggc tcagcgtggc tcagatcgcc
    24121 agcaccgtca gaatggccag cacgggtatg gaagtgagca aattccgcgg cagcggagtg
    24181 gatgagtatc ccctcatcgt caagctgcag gatcgctaca ccgaggagct ggaaaatctc
    24241 aaaaacctca agatcaggac ccgcatgggt gagatgatcg ccattaggga cctggcggag
    24301 tttgagattg ccacctccct ctcacggatc gagcaccgcg accagagaag ggagctcagc
    24361 gtcaccgcgg ccgcgggtac atataacaaa ggcggcaagc tggtgaaacg cagcgcttcc
    24421 gagatcaact ccctgctctt cggtggtggg atcagccgtg agcaaggtct tctgtcagat
    24481 tttgatcaac gcttcccggg ttataccatc accgccgggg gcgttcagga agagcaacgc
    24541 aagagctatt cttctctggg ctggctgttc ctgctggctc tgctgctcat cttcaccatc
    24601 ctggcctctc agttccgcag ctatgtacag cccctcatcg tgatgatgac cattcccttt
    24661 gccttcattg gggtgatttt gggtctcctg gtgacgggac tgcccttctc gctcaacacc
    24721 atgatctccg tggtggcgct ggcgggagtg gtggtcaaca acgccatcat cctgatcgat
    24781 tttatcaacc aggaacgcga aaaaggggcc gaccgctggc acgccattct caacagcggg
    24841 gcggtgaggc tgaggccaat cctgctcacc acagctacca ccgtggccgg aatgctgccg
    24901 ctggtgtttt ccggagaccc ttccgcgcaa gcctggagac cgctggccgt aagcttcacc
    24961 tttgggctgg tgttctcctc tgtcatcacc ctctttatca tcccggtgat ctacagcatg
    25021 gtggattcct tctttggccg gtttaagatg acccgcttca ccgagcacct caaattcacc
    25081 gaggccatcg agtgtaagac agacgaataa cctctgcaac taataactca agcgcctctc
    25141 atactggtcc gggaggcgct tgccgttttt acaggactat gtgaggtgcc ggctgaagag
    25201 ctggcgtgaa agccatattt acgccctttg cgcacaggcc aggaagagaa aatacagctt
    25261 ccaaacacgc tcttttttta taaataacgt gcttctcatg acattcatcc tgattataat
    25321 atcataatct tattatatgc tttaggagtc tattatggca tacccggcac cggattcaga
    25381 tccctgcggc gccttctgcg atcattacaa accaaggcgc aagcttgagg caaagatcag
    25441 gcaaaacctt gcccagggag aaatcccccg cggcatgggc tacttctatc cgaaaacctt
    25501 cagttattca gccccaatcc tgggcttgat gatcggagca tatgtcttca acgcgttcgc
    25561 aagctatctg ggcgtcagtg aaatccaggc tttagggatt cttatcccgg ttatggcact
    25621 gatgttgttt ctggtttttc ggggccggag agttattcta gtcactgatc accgtctgtt
    25681 gttcataggt cctggccgaa aaggcttgcc aagagacgtc gccatccagt ccgttaacaa
    25741 tgctgccatg aaaggctcca cgctgaaact ggagatcgac ggcaggaaag aatcctgggt
    25801 ggtggacaac tattcatcgc tcgcctggaa aaaaagccgt cggcaggagt ttatcgaggc
    25861 aataaagcag atcgggtgat atcgtcggcg cttcggcagt tttaccgcct tcagaatacc
    25921 tgtccttcta ttcacggtaa ataagagaag cctcattatt cgcttatcct tgcttagtca
    25981 cccgccgttg agatgccggg cgctctccgg cgaaaaaggc gggagagcgc acggcatctc
    26041 aacggcgggt gactaagcag gagtatggtg agcctgacgg acaggtatat cgaatggtag
    26101 cgtatctctt cgcttgcatc ggtgaaggag gaccaggatt atctagaaat agtcataaag
    26161 gtccaggtat cggtcttcta tgtctatcgc gtccaactgg aaatcagaca a
//
LOCUS       DAOSFN010000014        25785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1503_length_25785_cov_8.938010, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000014 DAOSFN010000000
VERSION     DAOSFN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 25785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25785
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1503_length_25785_cov_8.938010"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..463)
                     /gene="ssrA"
                     /locus_tag="PLO35_02015"
     tmRNA           complement(115..463)
                     /gene="ssrA"
                     /locus_tag="PLO35_02015"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(690..1616)
                     /locus_tag="PLO35_02020"
     CDS             complement(690..1616)
                     /locus_tag="PLO35_02020"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOH78467.1"
                     /translation="MNLIKAHLGRLELESPVTVASGTFGSEYFELRSDSDTALLPQSA
                     LGAYVSKTITPQPKAGNPHPRLWETECGLLNSIGLQNPGLQGFLNEELPRLRELLEIP
                     LIVSFSASSIDEFCQMLEALETSDGIDGYEVNVSCPNVAREGIAFGADPDVVYELTSR
                     LARITHRELMIKLSPNVSDIALIAQAAEGGGATSLALINTLWGMAIDWRTGASRLPRK
                     LCGYSGKGIKPVALALTYKAAQAVKIPILAMGGISCWQDALEFIYAGASAIAVGTANF
                     SDPGAPAQITHGLRDFCAVRGICLSEIVGSVR"
     gene            complement(1613..2329)
                     /locus_tag="PLO35_02025"
     CDS             complement(1613..2329)
                     /locus_tag="PLO35_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOH78468.1"
                     /translation="MKISEIDHLTGPYVIISVQDSGLAANSRPGMFFELRAPQQQDRL
                     FKPLSIYDVAGDRLSFFIKVLGAGTKALADMKDGDELELIGPLGNTFPLLENRKALLV
                     SGGVGYPPLAWLKKALPASTTTIHLHGGAGKDDVFPCEYPCTDNGSVGHKGFVTDLVP
                     ELIKGESIDLVYSCGPLPMLKKLASMCGNIEHYASMEAYMACGVGVCYGCAIPVGDAF
                     QRVCKEGPVFDARQIKWEEL"
     gene            complement(2344..3618)
                     /locus_tag="PLO35_02030"
     CDS             complement(2344..3618)
                     /locus_tag="PLO35_02030"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOH78469.1"
                     /translation="MKKLIKNGMVYSGGHIRKLDILIEDAHIKTVAPNLTEAADEVID
                     AAGKHVFPGFVDIHAHLRDPGQTYKEDIVSGTHAAAHGGITSLCAMPNTEPVVDNIAA
                     VDYVQRRAKDLGYAKVYVIGAMTKQSEGLEISEMATMKSGGIVAVSDDGKCVQNAKLM
                     LNCMRYAKNFELPVIVHSEDYTLGGKGQIHAGKVATRLGLTGIPGLAEEVIIARDIML
                     AESVGCRLHIAHISTARSIEMVRDAKNRGLNITCEVTPHHLILTDEACLTFDTNTKMK
                     PPLRSEKDRLACIEALIDGTIDCIATDHAPHADFEKVREFDHAPFGIIGFETAFPALY
                     HHLVKPGIVMLERVVEAYSTVPARLLGLPDNELKAGNAADIVIVDLQAQSDISEEFIL
                     SKSKNSPWLGHSLSGKIISTFMDGQISWQESE"
     gene            complement(3629..4567)
                     /locus_tag="PLO35_02035"
     CDS             complement(3629..4567)
                     /locus_tag="PLO35_02035"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOH78470.1"
                     /translation="MSDTPNFLGRNVFDLDDYSAEEILYILEAAKGMKEINLREYKKI
                     PTLRGKTVCTLFVENSTRTRMSFELAANRLSADVVSFQASVSALQKGESLQDTVYTLD
                     AMGIDLYCIRHSSPGSPQLVHKYSGKPVINGGDGRHAHPTQALLDIFSVWEKLGSLDG
                     LKITIVGDILNSRVVRSNLIGMKKLGAQVTVCGPRTLMPGGMEDVYGCKVEYNLREAL
                     RDADVVMGLRMQMERMTDGLFPSLEEYSKHYVLSKDTIKYAKKDALIMHPGPMNRGVE
                     ILPEIADGKQSIIVEQVANGVAVRMALMFLILGGKA"
     gene            4813..5901
                     /locus_tag="PLO35_02040"
     CDS             4813..5901
                     /locus_tag="PLO35_02040"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78471.1"
                     /translation="MKHLIIIALAAMLLLSACATSRQKLNPTANVALRTANMELANGN
                     VDVALKNYEIVLADRPDHAVALSKTGRIYYKKASEVPDSALENYTKAYDLISKSIKVF
                     ESFTPQTEADTKEIKELKDLRTSAWTRIFKIGEDQKEAGNTKDAIKIFEQVIELDPKR
                     DEPLRMLYDIYKDDETEPEKAESTLLKLLELKPNDPIILKTVGGHYYSKKQDYAKAVT
                     YYEQVKAIEPANVNTLLLIASSYYNLKQYQPALDNIQMALSIEPDNLDALNDAKTIAY
                     QLNNKELTLEYLQKALFIKEGEEELREITNLLFEMKRYQDMITYAEKWYEFNSSSKDA
                     VLLIITAAKELKNKTLEKKYNDIFKKMK"
     gene            5957..6892
                     /locus_tag="PLO35_02045"
     CDS             5957..6892
                     /locus_tag="PLO35_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH78472.1"
                     /translation="MNTRSRLSGSAYLYAGLAVLLWSTVSTAFKLSLRHLTPLGLLFF
                     SSLFASVFLAIYGFIIHKPNAFKGFLPHLKRSLPAGILNPFVYYLMLFNAYHRLPAQQ
                     AQVLNYTWAIVLPLMTLAVMKERLRALDLIALLLSFGGVVVISTRGDLSGLKLEDPLG
                     VAIAISTSLVWASYWILNMVDSRPAELKLMYNFISGTAFILLGSLLARVWGAGAWFVE
                     GASVAKGLAGGLYVGLFEMGLTFLLWYKALEKASSTAAVSNLIFVTPFLSLGFITLIL
                     KESIAPATIIGLIIIILSNGLQKASHRGAKTRNRQ"
     gene            complement(6886..7548)
                     /locus_tag="PLO35_02050"
     CDS             complement(6886..7548)
                     /locus_tag="PLO35_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78473.1"
                     /translation="MIELAEYDLADAPLIASCKVKAASRADNLVEEHHVVQYLHPITA
                     VVIGASNKAESSVIASACLDDGVPVIQRPSGGEAVVISPRTLLFSHIMLGSQLPRSRD
                     YFMLNLEVFQTELAKLGVRNLRFNGISDLCIGQKKILGCAIYRRPGMVLFQAVLNLAE
                     SPALIARYLAPPSRMPDYRQGRPHEEFVTSLEAEAYQIKAEDLDLPAAMKLVVKADLF
                     AY"
     gene            complement(7545..8606)
                     /locus_tag="PLO35_02055"
     CDS             complement(7545..8606)
                     /locus_tag="PLO35_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOH78474.1"
                     /translation="MTATDKELRWIRYLLILIALPVLAMILRTLAGIFIPLVLAIFLS
                     FLFAPLIDLLKRKRVHIIGVILIILLILILVFMLLSSLLFSAGTSIVTGLPRYQEKFT
                     ALMSNSYAWIEDLGQKLDVALEFVPAFDPRQMFSPGSFSVTRMVSDTMSGFINLVWNI
                     FLTMVFLLFIVGGATSMESRLKKALGEDQQERNLATVSRVQAQIQKYLITKTLISLAT
                     AIVGAVLMLIFGVDFVLVCALLLFAFNFIPNIGSIIASGIPIIICLLEYGLGFRVVGF
                     ALGITATQMLFGNIIEPKLQGDRLNLSPIMVLISLILWGWVWGIPGMFISVPLTSAIN
                     IILKEIDNKNIVSAIISSR"
     gene            complement(8626..9858)
                     /locus_tag="PLO35_02060"
     CDS             complement(8626..9858)
                     /locus_tag="PLO35_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOH78475.1"
                     /translation="MSRFRKLYDSRWSVVLGGFLVALMGGLSYSWGVFVEVLKQDYGW
                     SKMISTLPLSVFMVVFAIVMIPGGRLQEKISPGRQIRIGAFFFLAANLLSSLVVKFPH
                     KWWLLFSYGLIGGIGCGLSYSCIAPAIRRWFPDHPGLAVSLGVMGFGLAAFFFAPLKA
                     NFMLPSYGLHGTFIILAIVTFTITWLASWLIRFPSEWHLHLFGTVHLSGDDSMILANV
                     GPREMIRKPLFWLTWSAFLMVVYGSLLTIGILPSYGQTVVRLTGLQAAIPLSLFSLVN
                     GLSRPVIGYVSDKLGTLKVMSVVFLAQSLVFLLFPSHVTTYGPLLAASVILGFGIASS
                     LALFPVLTSEFFGVEHMGINYGILFSAYGFGAIAIQAGTYLHDLTGSYTPALLMAGVT
                     SLIGTLIMIYIRKTYKVS"
     assembly_gap    9884..9983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9984..11054
                     /gene="dinB"
                     /locus_tag="PLO35_02065"
     CDS             <9984..11054
                     /gene="dinB"
                     /locus_tag="PLO35_02065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOH78476.1"
                     /translation="MNKIIHVDMDAYFAAIEIRENPSLKGKCVIVGGSPNGRGVVSTC
                     SYEARKYGVRSGMASHQAWKLCPQGVFVHSSFALYKEVSNQIREVFFRHTELVEPVSL
                     DEAYLDVSASCSTLEEAVGIATSIKAEILAATRLTCSAGVSFNKFLAKIGSDLNKPDG
                     ITVISPDNARELLFALPIGKFHGIGRVTAAKMEKLGIHNGADLFNWELKDLMRRFGKV
                     GLFYYNVVRGIDSREVITSWDPKSISCESTFYTDVADVSELIGKLQDLVDRLVNRMSF
                     KGIQGKNIVIKIKYDNFDLITRSCALPEVTADKSIIFEYAQKLLVANWDETRKIRLLG
                     VGVGKLDLGNDDSNCQLCLPLD"
     assembly_gap    11154..11253
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11254..12903)
                     /locus_tag="PLO35_02070"
     CDS             complement(<11254..12903)
                     /locus_tag="PLO35_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH78477.1"
                     /translation="MAHFRFPNTSRIRFGQLTIIIAATLLTAACASSQGPLAGRVNGS
                     PIPRDNYISEYRGLFTKFWMTNERAPGIDEKNEIVRQTWRNITKDVILRQHFERYKIG
                     SSVVEAIDTLKANPPAYIVKSPVFQSNGVFDQALYQQSLLYGQPEDLSPLVRQYRDYY
                     VPVAKLKEALIDDQLLSSKQRQMIATAMTSSADLDLVYIDSSQLEVTVRDEDIKLYYD
                     THPSEFSLEQRYSVAYALMPLPASEEDVAAANALADSLFQYLKAGADSDSLLVEFRKV
                     NALISVKDSGFLKIDGLDPGLYNAFASIKIGEYLPPRPQDDGVAIHRLEQISKSLIGY
                     TTLWIPYLPGESTIAADRNRAAVNARLLRAVGYETASDELDLEFAHKTGITPGSLWLD
                     GRFETPDARQPQSLVKGYVPEPVFYPVLSAWLLIQVTDSQMDRQKPLDEVREAVQSKL
                     VLDQRRQFAMADAQAWLQSDAPSTLDISKLPSAQITTLKSQTLQNPQGPILDDGILFD
                     AMMARIKKQAPKVYQKDDYVIIPLIRALRSESSLKADPQKLR"
     gene            complement(12905..15532)
                     /gene="mutS"
                     /locus_tag="PLO35_02075"
     CDS             complement(12905..15532)
                     /gene="mutS"
                     /locus_tag="PLO35_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOH78478.1"
                     /translation="MTQSKDAKLTPMLRQFQDVKHKHPDKLILFRMGDFYETFFEDAR
                     QASKILNITLTTRNKNDADPVPLAGFPYHALDNYLDKLVKAGLKVVICEQVEDPKLAT
                     GLVKREVVEIITPGTVLDNALLESSASVYLACLYWEDLNRGIGVARLDLSTGEFAFTE
                     LPAEELLNELLRVKPAEIVVKDIESEQYVKALKLEFSPALTIFDSWQFRPHDASQVLK
                     EHFKVSSLEAYGANHHPLGTTAAGGALAYVRSLYQVPLNHISGLSFYSLSSYMQLDEI
                     SRRNLELTRSIRYGTRHGSLLSVIDQTRTPMGSRLLQQWLLHPLLDLKDITFRQDMIA
                     AFISNTAYLKDLRETLRDVGDIARLVSRLGALRINPRELLALRNFLVLNQIVRTKLTA
                     FAIEDFTPFTEQLAGFDDIASLIDDAIIDNPPVTVTEGGIFKSGFNADLDELLSLIHD
                     GKTWIAKLEEDERQKTGISTLKVGYNRVFGYFIEVSAAQKHKVPDYYLAKQTLVNSER
                     YVSPRLKEYEAKVLSAEEKIKNLEHELFRQLRIHLASHLERLQRLGEVIALIDVYASL
                     AWLAWQNRYCRPEFSSGRALLIEEGRHPVIEKIMEAEDFIPNSTALDYPETSIAIITG
                     PNMAGKSTYLRQVGLLVIMAQIGSWVPARRVVMPIFDRVFTRVGASDNLAQGQSTFLV
                     EMIETANILNCASRDSLILLDEIGRGTSTFDGLSLAWAIIEHIKKFKHALTLFATHYH
                     ELTELEHLYPDVKNYNVAVKEYNEQMIFLRKIERGGADQSYGIQVARLAGVPDRVIRR
                     AKEILRNLEEHEISARGRTASVRKKLVQNSAQVELFEVLMDKVEEKEPLLDEVRDLDL
                     DSLTPIQAWQFLKSLQSRI"
     gene            complement(15542..16684)
                     /locus_tag="PLO35_02080"
     CDS             complement(15542..16684)
                     /locus_tag="PLO35_02080"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOH78479.1"
                     /translation="MPFSNAKTSLRFSLTLGLLLFITALSANLRKEFQAVEKAYQQGK
                     LNEAAAQMLNLRASTDEEKALILYLTAVLKPKRADTLMHLQQAIEKYPSAYYGQLSML
                     HKAKLHILEREIAQAKNLLLKITSAQIAERYYWLAVCGEMSDDWAAAIANSETYLRLE
                     PGGERLEDCYFLMAEAYIGQKKYQSATAILAQLAAIKGYPTDVQYYHYLNGYAFQLAG
                     NWQEALSHYSQGIQVTRYTQLAYQIEDRLFELKHKHGSKIDLGFLFPYSDLDIPVETV
                     TPAEPEPAPLKELAPPAADLPLRLSAKPSSGLYVQTGRFGVEANARSVAYRVRQLSLP
                     SGYYEDKSNKHVPWVTFSGPYTNSDQQKKALELLKSNDIDCFSVRY"
     gene            complement(16696..17955)
                     /gene="rho"
                     /locus_tag="PLO35_02085"
     CDS             complement(16696..17955)
                     /gene="rho"
                     /locus_tag="PLO35_02085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOH78480.1"
                     /translation="MPIEENLFEQSQLQLTKIAKKIGVPGYTQFKGNDLVFKILEFQA
                     AQEGLAFVTGCLEISDEGFGFLRFPQNNYLPGRDDVYVSLTQVRRFGLKTGHVVSGPV
                     RSPKEGEKYFALLRVESVNYMAPTCLTELRPFDELTPYYPTERLNLEFKQDNFSTRII
                     NLFTPIGKGQRGLIVAAPRTGKTTLLQDTANAILANHPEVYLIVLLVDERPEEVTEMK
                     KILRPGNREVISSTFDESPRNHTAVAEMVLEKAKRMIELNQHVVIVLDSITRLARAYN
                     NITPSSGKVLSGGIDANGLIKPKKFFGSARNTEEAGSLTIIATALIDTGSKMDQVIFE
                     EFKGTGNMELVLDRSISDMRLYPAIDLVRSGTRREELLLTQNEINRMYVLRKYLKTIS
                     PIQGIEMLRKKMQATENNFELLNSMSN"
     gene            complement(17978..18691)
                     /locus_tag="PLO35_02090"
     CDS             complement(17978..18691)
                     /locus_tag="PLO35_02090"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH78481.1"
                     /translation="MKDVFDILNQFQVSHVLDAATGRGEFINVLRQKLGAYVQIIGVD
                     SNDKSVNYAQKFFPDNDVEIYRMELEELRFEDGYFDLVCISNSLHHLQHRDKVFAELL
                     RVLKPGGVFLVTEMYKDGQQTEAQMTHILMHHWVASIDRKCGIHHEETFSKDEILAII
                     HKLELKKLQTRDFYIPVDDPRQASNCEHLKRSCQETFKRLEGLEDPEILRSEGEAILA
                     RIEEIGCASASRLLLWGHK"
     gene            18959..19726
                     /locus_tag="PLO35_02095"
     CDS             18959..19726
                     /locus_tag="PLO35_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH78482.1"
                     /translation="MIEVLDLVKEFPKKDGTRFKAVDGVSFNAGDGEIVCLLGVNGAG
                     KTTTMRVLSTVFKPSAGTARIQGYDILTQADEVRRHLGFLSGDTGLYNRLRAREFITY
                     FGRLNGIGDPAIKRRMDEMADLLDMKDFMDKKIELLSSGMKQKVSIVRSIIHDPQVMI
                     FDEPTSGLDILTARNIVSFIRDCKIKGKCVLFSTHIMREAERLADRVVMIHRGKVLAN
                     GTLEEFRAQTGQTDLDDIFVHFVNQSSFEEELQAHEF"
     gene            19716..21713
                     /locus_tag="PLO35_02100"
     CDS             19716..21713
                     /locus_tag="PLO35_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HOH78483.1"
                     /translation="MSFNKALIVYRKELLEMMRDRRTLFATFIIPLILYPLLFIGFGS
                     IMSRQTAVLEERGAEIAFADSVNNHISAAIIAGIKTNKGYNLLPYNQDTQGLYNSGNI
                     QAIVSIGDSITAAGSSVYKVLVSYDSSKERNQLIYRKIKDSVTETKESSIATELSKRD
                     VDPALMELIRVDAIDTSSAQKRLGSYLGMILPYIMIMMLVAGAATVAGDLVAGEKERR
                     TLETLLVSSAQRNELVLGKYLTVITVAMVNVVVNLFSMGFSMQFLVGSQGIASSMTQM
                     PVVGFLILLAAMLPLATLFAALLMSISTFSRNLKEAASYQQPVMMISMLLGMISFIPS
                     VEINNLLALVPVVNIALLFKAVLINEYQLSHLLITVGSTLLLDIGAIWLTVKLFSTEA
                     MLFRTDEDSSLKNVRKEKRNLFNPFNGMVFFALALVVMYYLGSYFQREDMMKGLVQTQ
                     ILIILLPPLIILNIFKLKPKEILRITLPKPRELAIVPFIAVSGAVLVTLLMNVVDAIY
                     PFPDQYTEQLMGLFKMSDNLWVLLGIVALAPGICEEIMFRGFLMRFFEGNSKKMAVVI
                     SALLFGVFHLDPFRLIPTFLLGLILGYLTIRSGSIINSMLYHTLNNSVAVLVTTYASS
                     AWMKPLLRGSEGLQWWLAAPAALILAGSLWLFHKVTTPKGA"
     gene            21716..23551
                     /gene="glmS"
                     /locus_tag="PLO35_02105"
     CDS             21716..23551
                     /gene="glmS"
                     /locus_tag="PLO35_02105"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOH78484.1"
                     /translation="MCGIVGYIGSRNALPIVIEALKRLEYRGYDSSGCALIHEQDMQV
                     YKKQGKIIELERSLPEPNKCSGTIAIAHTRWATHGEPTELNAHPHLDCEGKLAIVHNG
                     IIENYQLLKQRLKELGHKFYSDTDTEVIVHLIEQYMSSTSCLEDAVREAMKRVEGTYG
                     LVVLSKDNPDKLIAVRKGSPIIIGIGDHEHFIASDVSAIVIHTQRVIYLQDNELCVIH
                     RDSFEITTLDKVQVEPKISLVDWAISAIEKGDFKHFMLKEIFEQPLTIGNGFRGRIND
                     QLASARLGGLNLPSPELRKVKQIHLIACGTSFHAALIGKYIIEDMARIPVHAEYASEY
                     RYRNPIIPDDTLVFVISQSGETADTLAALREAKAKGAIVLGITNVVGSTIARESDGGS
                     YIHAGSEIGVASTKAFTSQVTILTLLAILLGRQRSLSPMDGQKYINELEQIPAKIESI
                     LAQNDMIREIAASIKDSTNALYLGRGVNYPVALEGALKLKEISYIHAEGYPSAEMKHG
                     PIALIDEHMPVVAIATHDSLFDKVYSNLQEVRARKARLITIANAGSEELDKISEHVIR
                     IPETLSNLQPLLTVIPLQLLAYHVADLRGFDVDQPRNLAKSVTVE"
     gene            complement(23799..25133)
                     /locus_tag="PLO35_02110"
     CDS             complement(23799..25133)
                     /locus_tag="PLO35_02110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH78485.1"
                     /translation="MQYDLIVIGSGPAGYVGAIRAGQTGLKTLVIDKRYVGGMCLNWG
                     CIPSKTWLESARLYHKISRQAAEFGIDGIEADKLSFNWLQAKKRSSAVVAKLTRGIEY
                     LWKKNGVEFLKAEAKILSPNQVEADNRVFETQHIMIATGSRPAQLDGVPNLIGLERLS
                     ELEALPHQPLIWGHGPIAYEMLQFFRLLGKDPILALTHDILLPGLDPYIQSYVDKKLK
                     KEKLRIVPAAEIKFVANKAYYQDEEIAQDAVINCSWRQAVLPSMQIKLALEDGYILTD
                     EYYRSSVPNIFAAGDVNGRSYLAHAASAQALAIVNFIHGKATEEVPDTSWPINIYSDP
                     EIAQIGQTELQLKQAGIEYKSAEYSLSANGKALAEGASEGFLRLLYEPKYSQVLGVQI
                     VAANATDMIAEAAILMEMEGTTYDLAKAVHAHPTVAEVFMDLGSEAVKQLAE"
     gene            complement(25123..>25785)
                     /locus_tag="PLO35_02115"
     CDS             complement(25123..>25785)
                     /locus_tag="PLO35_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOH78486.1"
                     /translation="QNAVHMSVMDEVEVTELVEARKRLKPSFEAMGLKLSYLPFIIKA
                     VSLSLREHPILNAELDLENERIIHKDHINIGIAVDTDDGLVVPVIRDADQLSIQELTA
                     RINDFSVRAPKRQLSLDELKGGTFSITSYGSIGGYFAVPVINYPQAAIFGIGRLTQKP
                     VIKDGQIAIGNVLPISMSVDHRIVDGGEVARFLNLVLEYLKDPLLMLSVAPSDKGANH
                     AV"
BASE COUNT         5956 a         6672 c         6872 g         6085 t
ORIGIN      
        1 cggggaattg atcatattga tcatattaat caatcaaggt agcggtagag aggccatgga
       61 attgatgatc tcgcgccata cacaaaaggc caccctttcg gatggccgga aaactggtgg
      121 aggtggcggg gatcgaaccc gcgtccaaaa aaccgtttac gagtgaatct acatgcttag
      181 tcgtctttaa tctcggatca gatggcggag acgatcagac ccatctggcc ccagctcata
      241 gtcttaggcg aagcttatga gcgctgggct tcgcggcgtc cgcactgtta cgacgcccgt
      301 ttcacctccg gcggcccgaa gtggacggac gttagcgagc ttaagctgct aaggcgtagt
      361 tgttgtttgc agttaatttg cgtgctactt tataacggag tggatagcgt ctccggcatg
      421 catctctcgc accctggttc cctgtcgaaa ccatgtcacc ccctatatac ttgcaaaaga
      481 acaacctatg acgggctgaa gcaaagtgat tgttttcaga cccgatttct gaagacaatt
      541 taagcgaggt gctaaggatg tcaagcccaa attcttaccg agcccggcgt cttatgcctg
      601 cctcgatctc gtcgaactca ccccccattg agttcgggat gggttggagg tgctataccg
      661 gcgccagaga gatacggctc aggaaggatt cagcggacgc ttcccacgat ttccgaaagg
      721 cagatgcctc tcacggcgca gaaatccctg agcccgtgcg tgatctgtgc aggagcaccg
      781 ggatcgctga aattggcggt tcccacggca atcgcggaag ccccggcata gataaactcc
      841 agagcgtcct gccagcagga gatgcctccc atggcgagga tggggatctt cacggcttga
      901 gcggctttgt aggtgagcgc cagggcaacg ggtttgatgc ccttaccgct gtagccacag
      961 agtttgcggg gcaatctgga ggctcccgtg cgccagtcta tcgccatgcc ccacagggtg
     1021 ttgatgagag ccagggaggt agcaccaccc ccttcggcag cctgagcgat gagagcgata
     1081 tcggagacgt tggggctgag cttgatcatc agctctctgt gcgtgatcct ggcaagcctg
     1141 gatgtaagtt catataccac atcgggatcg gcgccaaaag cgatgccttc cctggccaca
     1201 ttggggcagg agacgttcac ttcatagccg tcgatgccgt ccgaagtttc cagggcctcc
     1261 agcatctggc aaaactcgtc tatggaagag gcggagaagc tcacgatcag gggaatctcc
     1321 aaaagctcac gcagacgcgg gagttcttca ttcagaaagc cttgcagacc tgggttttgc
     1381 agcccgatgg agttcaggag cccgcattcc gtctcccaga gcctgggatg aggatttccg
     1441 gctttgggct gaggcgtgat cgtcttgctg acatatgctc ccaacgcgga ttgaggcagc
     1501 agagcggtgt cgctatctga gcgcagctca aagtattcgc tgccaaaggt gccggaggcc
     1561 acggtgacgg gactctcaag ctccagcctg cccagatgcg cctttatcag gttcacagct
     1621 cctcccactt gatctgtcgg gcatcgaaga ccggcccttc cttgcagacc ctctgaaaag
     1681 catcacccac aggaatggcg caaccgtaac agacgcccac gccgcaggcc atataggctt
     1741 ccatggaggc gtaatgctcg atgtttccac acatcgatgc cagctttttc agcatcggaa
     1801 gcggtccaca gctatagacg agatcgattg attccccttt aatcagctct ggaactagat
     1861 ctgtcacgaa gcccttgtgc cccaccgagc cgttatccgt gcagggatat tcgcatggga
     1921 agacgtcgtc tttcccagct ccgccgtgta ggtgaatcgt ggttgtggaa gccgggaggg
     1981 cttttttcag ccatgccaga ggtggatagc ccactccccc actcacgagc agggcctttc
     2041 tattctcaag gagtggaaag gtgtttccca ggggaccgat aagctccagt tcatcgccgt
     2101 ctttcatatc tgccagagct ttggtgcccg ctccgaggac cttgatgaag aagctaagtc
     2161 tatctccggc aacatcatag atgctgaggg gcttgaacag cctgtcctgc tgttggggcg
     2221 ctctcagctc aaaaaacatc ccaggccggc tgttggcagc cagcccggaa tcctgaaccg
     2281 agatgatcac ataggggcca gtgaggtgat ctatttcact tattttcaaa gtcttacgcc
     2341 aggtcactcg ctttcctgcc agctgatctg gccatccata aaggtggaga ttatctttcc
     2401 ggaaaggctg tgtcccagcc aggggctatt cttgctcttg gagaggataa attcttcgct
     2461 gatatcgctt tgcgcctgca gatcgacgat cacgatatcc gcggcgttgc cagctttgag
     2521 ctcattgtcc ggcagtccca gcagcctggc aggaactgtg gaataggcct ccacgacgcg
     2581 ctccagcatc acgatcccgg gtttgacgag atggtgatac aaggctggga aggcggtttc
     2641 gaaaccgatg atgccaaagg gagcgtgatc aaactcacgc actttttcaa aatcggcatg
     2701 gggagcgtga tcggtggcga tgcagtctat ggtcccgtct atgagggctt cgatgcaggc
     2761 caggcggtcc ttttcagacc gtaggggcgg cttcattttg gtgttggtgt cgaaagtgag
     2821 gcaggcttca tcggtgagga tgagatggtg gggcgtgacc tcgcaggtga tgttcaggcc
     2881 gcggtttttg gcgtccctaa ccatctcgat ggagcgggcg gtgctgatat gggcaatgtg
     2941 gagcctgcag cccacgcttt ccgccagcat gatgtcccgg gcgattatga cctcttcagc
     3001 cagcccggga atacctgtca aaccaaggcg ggtggctact ttaccggcat gaatctgtcc
     3061 cttgccgccc agcgtgtaat cttccgaatg cacgatcacg ggcagctcaa agtttttggc
     3121 gtagcgcatg cagttcagca tcagcttggc gttttgcacg catttgccgt catcggagac
     3181 ggctacgatg ccgccggact tcatggtggc catttcgctg atttcgaggc cttcgctctg
     3241 cttggtcatg gcgccgatca catagacttt ggcgtatccg aggtctttgg cgcggcgctg
     3301 cacataatcc acggcggcga tgttgtccac caccggctcg gtgtttggca tggcgcagag
     3361 gcttgtgatg cctccatgag cggcggcgtg ggtgccggaa acgatgtctt ccttgtaggt
     3421 ctggccggga tctctgagat gggcgtgaat atccacaaag ccaggaaaga cgtgctttcc
     3481 ggccgcgtcg ataacttcat cggccgcttc cgtgaggttg ggagcgacgg tttttatgtg
     3541 agcgtcctcg atgaggatgt cgagcttgcg gatgtggcct ccgctgtaaa ccatgccgtt
     3601 tttgataagc tttttcatga taacctcctc aagcctttcc acccaggatc aggaacatga
     3661 gcgccatccg cacggccact ccgtttgcca cctgttccac gatgatgctc tgtttgccgt
     3721 ccgcgatctc tggcaggatt tccactccgc ggttcatggg gccgggatgc atgatgagag
     3781 cgtccttttt ggcgtatttg attgtgtctt tggagagcac atagtgtttg ctgtattcct
     3841 ccagggaggg gaacaagccg tctgtcatgc gctccatctg catgcgcagt cccatcacga
     3901 cgtccgcgtc ccgcagggct tcacggaggt tatattccac tttgcagcca tagacgtctt
     3961 ccatgccgcc tggcatgaga gtgcggggtc cgcagaccgt cacttgagct cccaacttct
     4021 tcattcctat aaggttggag cgcaccaccc ggctgttgag gatgtctccc acgatggtga
     4081 ttttgaggcc atccagcgag cccagctttt cccacacgct gaaaatatcc aacagagcct
     4141 gggtgggatg ggcgtgccgt ccgtctccac cgttgataac cggctttccg gagtatttat
     4201 gcacgagctg cggactgccc gggctgctgt gccggatgca gtaaagatcg atgcccatgg
     4261 cgtccagagt gtaaacggtg tcttgcaggc tttccccttt ctgcagtgcc gaaacgctgg
     4321 cctggaagct cacgacgtcc gcgctgagac ggttggcggc cagttcaaag ctcatgcggg
     4381 tgcgggtgct gttttccacg aagagagtgc agacggtttt tccgcgcaga gtggggattt
     4441 tcttgtactc acgcaggttg atttctttca tgcccttggc ggcctcgaga atgtagagga
     4501 tctcctcagc ggagtagtca tcgaggtcaa agacgttacg gcccaggaaa ttaggcgtat
     4561 cagacatact ttctcctttc cggctcactg gctcggttta aaggtaaatt ttgtgtcact
     4621 cttacaacgg gggatttgta gtcaagtgtt tttgcgccaa gatttgatcc atgcggctca
     4681 gtagattgac gggcagtgag ttatgattgc ggttataatg cggatatcgg tcaggtcagg
     4741 aaaaatgctt gacttaaaag gcaaagcgat aatactgcct ttcaaagtaa aatcacaaac
     4801 aggagactat acatgaaaca cctgattatc atagctttag ctgccatgtt gctgctcagt
     4861 gcctgcgcca cgagccgtca aaagctgaat cccacggcca atgtcgccct ccgcactgcc
     4921 aatatggagc tggccaatgg aaacgtggat gtggctctca agaattacga gatagtgctc
     4981 gccgaccgtc ctgatcatgc cgtcgcgctc agcaagacag gccgcattta ttacaaaaaa
     5041 gcctcagaag tacctgacag cgccctggag aattatacca aggcttacga cctgatcagc
     5101 aaatccatca aagtctttga gagttttact ccccagaccg aagccgacac caaagagatt
     5161 aaagaattga aagacctgcg caccagcgcc tggacgcgta tcttcaaaat cggagaagat
     5221 cagaaggaag ccggcaacac caaagacgcg atcaaaatct tcgagcaagt gattgaactc
     5281 gaccccaaac gcgatgaacc cctcaggatg ctttatgaca tctataaaga tgatgaaact
     5341 gagccggaaa aagcggaaag cacccttctc aaactgcttg agctgaaacc caacgatcct
     5401 atcattctta aaacggtggg cggccactat tatagcaaga agcaagacta cgcgaaggct
     5461 gttacctatt atgagcaagt gaaggcaatt gaacccgcta acgtgaacac gctgctcttg
     5521 atcgcctctt cctactataa tctcaagcag tatcaaccag ccctggataa cattcagatg
     5581 gccctcagca tcgagcccga taatctggat gcccttaatg acgcaaaaac cattgcctat
     5641 cagctcaaca ataaggagct gacccttgag tatctgcaaa aagctctgtt cattaaggag
     5701 ggtgaagagg aactgcgcga gatcacaaac ctgcttttcg aaatgaaacg ttatcaggat
     5761 atgatcacct acgccgagaa gtggtatgag ttcaacagca gcagcaaaga cgccgtgctc
     5821 ttgatcatca ccgccgccaa agagctgaaa aacaaaactc ttgagaagaa atacaacgac
     5881 atcttcaaga agatgaaata aaccagtcct tctattgaag cctgtcgtgc cggtcatgac
     5941 aggcttttct tttttgatga atacgcgctc cagactctcc ggttcggcct atctctacgc
     6001 gggattggcg gtgttgctgt ggtccacagt atccaccgcg ttcaagctca gtctgcgcca
     6061 tctgaccccg ttgggattgt tgtttttctc atccctgttc gcctctgtgt ttctggcaat
     6121 ctatggattc ataatccaca agcctaacgc tttcaaaggc tttttgcccc atctgaagcg
     6181 ctccctgccg gcaggtatcc tcaacccctt tgtctattat ctcatgctct tcaatgccta
     6241 ccaccgtctg cccgctcaac aggcccaggt gctcaactac acctgggcaa tcgtgttgcc
     6301 tttgatgacg ctggcagtaa tgaaggagcg gctgagagcg ctggacctca tagccttgct
     6361 gctcagcttc ggaggcgtgg tcgtgatctc cacccggggc gatctgagcg gcctcaaact
     6421 ggaagaccct ctgggagtgg cgattgccat cagcacttca ctggtctggg cctcatattg
     6481 gatattgaat atggtggatt cccgtcccgc ggagctgaaa ctgatgtaca attttatctc
     6541 cggcactgca tttatcctct tggggagttt gctggcgaga gtctggggag ccggggcctg
     6601 gtttgtggag ggagcatcgg tggcaaaagg tctggcgggt ggactctatg tgggattgtt
     6661 tgagatgggg ctcacctttt tattgtggta caaagccctg gaaaaggcca gcagcaccgc
     6721 tgcggtttcc aatctgatct ttgtgacccc gtttctgtct ctggggttta tcactctcat
     6781 cctcaaggag agtatcgctc cggccacaat tatcggcctg atcattatta tcctcagcaa
     6841 cggactccag aaagcctccc accgaggggc caaaacccgc aatcgtcaat aagcaaacag
     6901 atcagctttc accacgagct tcatcgcggc aggaaggtcc aagtcttccg ctttaatctg
     6961 ataagcctcc gcctcaagag aagtgacaaa ttcttcgtgg ggccggccct gacgataatc
     7021 tggcatcctg gaaggcgggg ccaggtatct ggcgatcagg gcgggagact ctgccaggtt
     7081 caataccgct tgaaacaaca ccatcccggg tctgcgataa atggcacaac ccagaatctt
     7141 tttttgcccg atacagaggt ccgatatccc gttaaacctg aggtttcgca ctcccagttt
     7201 agccagttcc gtctgaaaaa cttccagatt cagcatgaaa tagtccctgg agcggggaag
     7261 ctgggagccc agcataatat gactgaacag cagcgtcctg ggagagatca ctacggcttc
     7321 tccaccactg ggacgctgga tcacaggaac gccatcatcc aggcacgcgg aagcgatgac
     7381 ggaagattcc gctttgttgc tggcaccaat caccaccgcc gttatgggat ggagatactg
     7441 gaccacatga tgctcttcca ccagattatc agccctgctg gctgctttta ccttgcagga
     7501 cgcgatcagg ggggcatccg ccagatcgta ttcagcgagt tcgatcatcg gctgctgata
     7561 atggccgaaa cgatgttttt gttgtctatc tccttgagta tgatgttgat ggctgaggtc
     7621 agcggtacgg agataaacat gccaggtatc ccccacaccc aaccccacag gatcaaggat
     7681 atcaagacca taatcggcga gagatttagc ctgtcgccct gtagcttagg ctcgatgatg
     7741 ttgccaaaca gcatctgggt ggcggtgatg ccgagggcaa aacctactac cctaaatcct
     7801 aacccatact ccagcagaca gataatgatt ggaatcccgg aggcgatgat ggaaccgata
     7861 ttgggaatga agttgaacgc gaagagcagc agcgcgcaga ccagaacaaa atcaaccccg
     7921 aagatcagca tcagcacagc tcccacaatg gcggtcgcga gactgattaa ggtcttggtg
     7981 atcagatatt tctggatctg ggcctgcact ctggagacgg tggcgagatt gcgctcctgt
     8041 tgatcttctc ccagagcttt tttcagccgc gattccatgc tggtggcccc tccaacgata
     8101 aacagcagaa acaccatcgt cagaaagata ttccacacca gattaatgaa accgctcatt
     8161 gtgtccgaca ccatcctggt cacggaaaaa ctgcctggag aaaacatctg gcggggatca
     8221 aaggcaggaa cgaattccaa cgcgacatcc agcttctgcc ccagatcctc tatccacgcg
     8281 taggaattgg acatcagggc cgtaaacttt tcctggtagc gcggcagacc ggtcacaatg
     8341 ctggtcccgg cactgaataa aagggacgac agcagcatga acaccagaat caggataagc
     8401 aggataatca ggattacccc aattatatgc actcgcttac gtttgagcag gtcgatcagc
     8461 ggagcaaaca ggaaggagag gaaaatcgcc agcaccaggg gaatgaatat tcctgccaga
     8521 gtcctcagga tcatagccaa cactggcagc gcgatcagga tcagcagata gcggatccag
     8581 cgcagttctt tatcggttgc agtcataatt acgctcccgg gtaaatcagg acactttata
     8641 agtcttacgg atatatatca ttatcagagt tcctatgaga gatgtcaccc ccgccatcaa
     8701 cagtgccgga gtgtagctgc cggttagatc gtgaagatag gtgcccgcct ggatggcaat
     8761 ggccccaaat ccataggcgg agaataatat tccgtagttt atccccatgt gctccacgcc
     8821 aaaaaactca gaggtcaaca ccggaaacaa cgccagagaa gaggcaatcc caaagcccag
     8881 gatcaccgac gccgccagca aaggtccata ggtcgtgacg tgactgggaa acaacaagaa
     8941 tacaaggctt tgagccagaa acactacact cattaccttc agagtgccca gtttatcact
     9001 cacatatccg atcactggac ggctgaggcc gttcaccaaa gaaaacaggg aaagcggaat
     9061 tgccgcctga agccctgtga gcctgacaac cgtctgcccg taggaaggca ggatcccaat
     9121 cgtgagcaga ctgccataca cgaccatcaa aaaagcgctc caggtgagcc agaacaaagg
     9181 tttgcgaatc atctcgcggg gcccgacgtt cgcgagaatc atggaatcgt ctccagacag
     9241 atggacggtg ccaaacagat gcaggtgcca ttcggagggg aacctgatca gccaggaagc
     9301 aagccaggtg attgtgaaag ttacaatcgc cagaatgata aaggtgccgt gcaaaccata
     9361 gctgggcaac atgaaattgg ccttgagggg cgcgaaaaaa aaagccgcca gcccaaaacc
     9421 catcactccc agagatactg ccagacccgg atggtctgga aaccagcgtc taatggctgg
     9481 ggcgatgcat gaatagctga gtccgcagcc gattccgccg atcaaaccgt aactgaacag
     9541 cagccaccac ttgtggggaa acttgacgac taaagatgac agcaggttcg ccgccaggaa
     9601 gaaaaaggcc ccgatccgaa tttgacgccc gggagaaatc ttttcctgca aacgtcctcc
     9661 ggggatcatg acaatcgcaa acaccaccat aaataccgac agcggcaggg tggagatcat
     9721 ctttgaccag ccgtaatcct gcttcagcac ttccacaaaa acaccccagg aatatgacaa
     9781 accacccatc agggctacca ggaaacctcc caataccaca ctccagcggg aatcataaag
     9841 ctttcggaat cttgacatat ctctcccttc agacctggtg gtcnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnatgaaca agatcattca cgtggatatg gacgcctatt
    10021 ttgccgctat agagatcagg gaaaacccgt ctctgaaggg caaatgcgtg atcgtgggcg
    10081 gcagtcccaa cggtcgtgga gttgtgtcca cctgttctta tgaagcgcgc aagtatggag
    10141 ttcgcagcgg gatggcctcc catcaggcct ggaaactttg cccccaggga gtgtttgtgc
    10201 actccagttt tgcgctctat aaggaagttt cgaatcagat cagagaagtt tttttccgcc
    10261 acacggagct ggtggaaccc gtctcactgg atgaggccta cctcgatgtc agcgcttcct
    10321 gctccaccct tgaagaagcg gttgggatcg ccacatcaat caaagctgaa atcctggctg
    10381 ccacccggct aacctgttct gcgggagtgt ctttcaacaa attcctggca aaaatcggct
    10441 ccgacctgaa taaaccggac gggataactg tgatatctcc tgataacgcg cgggaacttc
    10501 tgttcgcgct ccctatcggc aagtttcacg gcattggcag agtcacagcg gcgaaaatgg
    10561 aaaaactggg catccacaac ggagcggacc tcttcaactg ggagctcaaa gacctgatga
    10621 gacgctttgg caaggtgggg ctgttttact acaacgtggt gaggggcatc gacagccgcg
    10681 aggttatcac cagttgggac cccaaaagta tcagttgtga aagcaccttc tacaccgacg
    10741 tcgcggatgt gagtgagctc atcggcaagc tgcaggacct cgtagaccgt ctggtgaacc
    10801 gcatgtcttt caaaggcatc cagggcaaga atatcgtgat taagatcaag tatgacaact
    10861 ttgacctcat cacccgcagt tgtgccttgc cggaagtcac tgccgataag agcattatct
    10921 ttgaatacgc ccaaaaactg ctggtggcaa actgggatga gacccgcaaa atccgcctgc
    10981 tgggagtggg agtgggaaaa ctggacctcg gaaacgacga ttccaattgc cagctttgct
    11041 tgcctctgga ttagagttac gtttcccgca ggttctgcag atagtgtatg ttaagaacgg
    11101 ttaagaaggt tcaggaggca agtggctcaa ccctctaaac cgtcataaaa actnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgcagt ttttggggat ccgctttcag
    11281 agagctttcg gagcgcaagg cgcggatgag ggggatgatg acgtaatcgt ccttttgata
    11341 gactttggga gcttgttttt taatcctggc catcattgcg tcaaagagaa tcccgtcatc
    11401 gaggataggc ccctgggggt tttgcagagt ttgggacttc aacgttgtga tttgggcgga
    11461 gggcagtttt gagatgtcga gcgtggaagg cgcgtcgctc tgaagccagg cttgcgcgtc
    11521 tgccatggcg aactggcgtc tttgatcaag gaccagtttg gattggacag cttccctgac
    11581 ttcatccaga ggtttttgcc tatccatctg actgtcagta acctggatca acaaccaggc
    11641 agagaggacc gggtagaaaa ctggctcggg aacgtaaccc tttaccagag actgaggctg
    11701 ccgggcgtcc ggagtctcga atcttccatc cagccaaagg ctgccaggcg tgatgcctgt
    11761 cttgtgagca aactccaggt ccagctcgtc ggaagccgtt tcataaccca cggccctcag
    11821 cagtctggcg ttcactgcgg ctcggttgcg atctgcggcg atggtgcttt cgccaggcaa
    11881 ataaggaatc cacagggtcg tgtagccaat caggctcttg ctgatttgtt ccaaccggtg
    11941 aatggccact ccatcatcct gggggcgggg aggcaaatac tctccgatct tgatggacgc
    12001 gaaggcgttg tagagccccg gatcgagccc atctatcttg agaaatccgc tgtctttgac
    12061 gctgataagg gcgttgacct tcctgaactc caccagcaga gaatcggagt ccgctcccgc
    12121 tttcagatat tggaacaagc tgtccgccag agcgttggca gcagcgacat cttcctctga
    12181 ggctggcagt ggcatcaaag cgtaggctac gctataacgc tgctcgaggc tgaattcact
    12241 ggggtgggta tcgtaataca gcttgatatc ttcatcccgg acagtcactt ccaactggct
    12301 ggaatcgata tatacaagat ccaaatcggc gcttgaggtc atggctgtgg caatcatttg
    12361 acgctgtttg gagctgagca gttgatcgtc tatcaacgcc tccttaagct tggccacggg
    12421 aacatagtaa tcacgatact gccgcaccag gggagaaagg tcctctggct gaccatagag
    12481 cagagactgc tggtagaggg cctggtcgaa aactccgttg gactggaaaa caggggattt
    12541 gacgatatag gcgggcggat tggctttaag agtatcgatg gcctcgacta cgctgctgcc
    12601 gatcttgtaa cgctcaaagt gctgccggag gatgacgtct ttggtgatgt tgcgccaggt
    12661 ttgacgtaca atttcatttt tctcgtctat ccccggagcc cgttcgttgg tcatccagaa
    12721 tttggtaaac aggcctctgt actcagagat gtaattatct ctggggatgg gagatccatt
    12781 cacgcgcccc gcgagggggc cctgagagct ggcgcacgct gcggtcagca atgtcgcggc
    12841 tatgataatc gtaagctgcc cgaatctgat acgggaagtg tttgggaatc tgaaatgagc
    12901 cattctatat cctgctttgg aggcttttaa gaaactgcca tgcctgaatt ggcgtgaggg
    12961 aatcgaggtc cagatctctg acttcgtcca gaagtggttc cttctcttct actttgtcca
    13021 tcaggacttc aaagagttcc acttgcgcgg agttctgcac cagttttttt ctgactgagg
    13081 cggtccttcc gcgagcgctg atctcgtgtt cctccagatt gcggaggatt tctttggccc
    13141 tgcggatgac gcgatcaggc actcctgcca gccgcgcgac ctggattccg tagctctgat
    13201 cggcccctcc gcgttcgatt ttacggagga agatcatctg ttcgttgtat tctttcaccg
    13261 ccacgttata gtttttcacg tccggataga ggtgctccag ctcggtgagt tcgtgataat
    13321 gagtggcaaa gagggtgagg gcgtgtttga atttcttgat gtgttcaatg atcgcccatg
    13381 ccaggctgag cccgtcaaaa gtagaagtac cgcgcccgat ctcgtccagc aggatcagtg
    13441 aatcccggct ggcgcaattg aggatgttgg cggtttcgat catttccacc aggaaggtac
    13501 tttgcccctg tgccaggttg tcggaagctc ccacccgggt aaacacgcgg tcgaagattg
    13561 gcatcaccac acgccgggcg ggcacccagg aaccgatttg ggccatgata accagcaatc
    13621 ccacctgacg cagataggtg gatttgcccg ccatattggg gcccgtaatg atggcgatcg
    13681 aggtctcagg ataatccaga gcggtggaat tgggaataaa atcctcagct tccatgatct
    13741 tttcgatcac gggatggcgt ccctcctcta taagaagagc tcgaccggag gagaattccg
    13801 gacggcagta gcgattttgc cacgccagcc aggccagaga cgcatagacg tcgatcagag
    13861 cgatcacttc acccagacgc tgcaagcgct ccaaatggga cgccaggtgg attctgagct
    13921 ggcggaacaa ctcatgctcg aggtttttga tcttttcttc cgcgctcagg actttggcct
    13981 cgtattcttt gaggcggggg gaaacatagc gttcggaatt cacgagggtc tgcttggcca
    14041 gataataatc gggcaccttg tgtttctggg cggcggaaac ttcgatgaaa tacccaaaga
    14101 cccggttgta tcctactttg agagtgctga tgccagtctt ttggcgctcg tcttcctcca
    14161 gtttcgctat ccaggtcttg ccgtcgtgga tgaggctgag caattcgtcg agatcggcgt
    14221 tgaaaccgct tttaaagatg ccgccttccg tgacggtgac aggggggttg tcgataatgg
    14281 cgtcgtcgat caggctggcg atatcatcaa agccggccag ttgctctgtg aagggggtaa
    14341 aatcctcgat ggcgaaggct gtcagcttgg tgcgcactat ctggttgagc accagaaaat
    14401 ttctgagggc cagcagctcg cggggattga tccttagagc tccgaggcgt gaaaccagcc
    14461 tggcaatatc gcctacatcc ctcagagtct ctctgaggtc tttgagatag gcggtattgg
    14521 agataaaggc cgcgatcatg tcctgccgga aagtgatatc cttcaaatcg aggagaggat
    14581 gtagcagcca ttgctgcagc agcctgcttc ccatgggagt acgggtctga tcgatgacac
    14641 tcaacaaact cccatgcctg gtgccgtagc ggatcgagcg cgtaagctca agattgcggc
    14701 ggctgatctc gtccagttgc atatagctgc tcagcgagta gaaactgagg ccggagatat
    14761 gattcagggg cacctgatag agtgaacgca cgtaggccag agctccaccc gccgccgtgg
    14821 tccccaaagg atgatgattg gccccgtaag cctcaagaga gctgactttg aagtgttcct
    14881 tcaacacctg ggaagcgtca tgagggcgaa actgccaact gtcaaaaatc gtgagcgcgg
    14941 ggctgaattc cagtttgaga gcttttacat actgctcaga ttcaatatcc ttgactacga
    15001 tctcggcagg tttgacgcgc agaagttcgt ttaagagctc ttctgccgga agctccgtga
    15061 aggcaaattc accggtggag agatccagcc tggcgactcc gatcccgcga ttcagatctt
    15121 cccagtaaag gcaggcaaga tagacgctgg cactgctttc caggagcgca ttgtcgagca
    15181 ccgtgcccgg agtgatgatt tccaccactt cgcgtttgac cagtcccgtc gccagtttgg
    15241 gatcttccac ttgttcgcag atgaccacct tgaggcccgc tttcactagt ttgtcgagat
    15301 agttgtcgag agcgtgatat ggaaatcccg ccaggggaac tggatccgcg tcgtttttat
    15361 tgcgggtggt gagggtgata ttgaggattt tggatgcctg gcgggcatcc tcaaaaaagg
    15421 tctcatagaa gtcacccatg cgaaagagaa tgagcttgtc cggatgctta tgcttcacat
    15481 cctgaaactg ccggagcatg ggagtgagct tagcgtcttt ggattgagtc atttatgggg
    15541 gtcagtaacg cacggagaag caatctatgt cattactttt gagcaattcg agggcttttt
    15601 tctgttgatc agagtttgta tagggtccac tgaatgtaac ccagggaacg tgtttattgc
    15661 tcttatcttc gtagtatccg gaggggaggc tgagctgtct gacccggtag gcgacggaac
    15721 gggcgttggc ttccactccg aagcgccctg tctgcacata gagtcccgag gagggtttcg
    15781 cgctcaacct caaaggcaga tcggcagcgg ggggggcaag ttctttgagc ggcgcgggct
    15841 caggttcagc cggagtaacg gtttcaaccg gaatatccag atcgctgtag ggaaagagga
    15901 aaccaagatc gatttttgag ccgtgcttgt gcttcagttc aaagagccgg tcttcgatct
    15961 ggtaggccaa ctgagtgtag cgagtgacct gtatcccctg gctgtaatga gatagggctt
    16021 cctgccagtt gcctgccagc tggaaagcgt agccatttaa gtagtgataa tactgaacgt
    16081 cagtgggata gcccttgatg gcggcaagct gggccaggat ggcagtagcg ctctgatact
    16141 ttttttgccc aatatacgct tcagccatca gaaaataaca atcttcgagg cgttctccac
    16201 ccggctccag tctcaggtag gtttcggagt tggcaatcgc ggccgcccaa tcgtctgaca
    16261 tttcaccgca aaccgccagc cagtaataac gttccgcgat ctgggcggag gtgattttga
    16321 gcaacaggtt ttttgcctgg gcaatctccc gctccaggat gtggagcttg gctttgtgaa
    16381 gcatgctcag ctggccgtag taagcagagg gatatttctc aatagcctgc tgcaggtgca
    16441 tcaaagtgtc ggcccgtttg ggcttcagca ccgcggtcag atagaggatc agggcctttt
    16501 cttcatccgt cgaagctctc agattgagca tctgagcggc tgcttcgttc agcttacctt
    16561 gctgataggc tttttccacc gcttgaaact ccttgcgcaa attggcagaa agagcggtga
    16621 taaatagcag taacccaagc gtgaggctga atctcaggct ggttttggca ttgctgaatg
    16681 gcatcgaatg acgcctcaat tgctcatcga attgagcagc tcgaagttgt tttctgtggc
    16741 ctgcatcttt ttgcgcagca tctcgatgcc ctgaatcggg ctgatggtct tgaggtattt
    16801 gcgcaacacg tacatgcggt tgatctcatt ctgcgtaagc agcagttctt ctctgcgcgt
    16861 accgctgcga acgagatcga tggccggata tagccgcatg tcagaaatgc tgcggtctag
    16921 cacgagttcc atgtttccgg tacccttaaa ctcctcaaaa atcacctggt ccatttttga
    16981 gccagtatcg atcagagcgg tggcgataat cgtgagcgaa ccggcttcct cagtgttgcg
    17041 ggcggaacca aagaactttt tgggtttgat gagaccgttg gcgtcgatac caccgctgag
    17101 cactttgccg ctggagggcg tgatattgtt atacgcacgg gccagacggg tgatgctgtc
    17161 gaggacgatc accacatgct gattgagctc gatcatgcgc ttggcttttt ccagcaccat
    17221 ttcggccaca gcagtgtggt ttctgggaga ttcgtcgaag gtggagctga tcacttcgcg
    17281 gttgccgggc ctgagaatct tcttcatttc ggttacttct tctggacgct catccaccag
    17341 caatacaatg agatatacct ctggatggtt ggcaagaatg gcgttggcag tatcctgaag
    17401 gagggtagtc ttaccagttc tgggagcagc tacgatcagc ccccgctggc cttttccgat
    17461 gggagtgaaa agattgatga tgcgcgtgga gaagttatcc tgcttaaact cgaggttgag
    17521 ccgttcggta gggtagtagg gcgtcaattc atcaaaggga cgcagttctg tgaggcaggt
    17581 gggggccatg tagtttaccg attccaccct cagcagggca aagtacttct ccccctcttt
    17641 gggagagcgc accggtccag aaacgacgtg gccggttttc aatccaaacc ttctcacctg
    17701 agtgagtgag acatagacgt cgtcgcgccc ggggaggtag ttgttttggg gaaacctgag
    17761 aaagccgaag ccttcatcgc tgatttccag acaaccagtc acgaaggcca gaccttcctg
    17821 agccgcttgg aactctaata ttttaaacac caaatcgttg cccttgaatt gcgtgtagcc
    17881 gggcacgccg attttcttgg cgattttagt gagctgaagc tgactttgtt caaacaggtt
    17941 ttcttcgatg ggcattatgt atctcctagt taatatgcta tttatggccc caaagcaaga
    18001 gccggctggc gctggcgcaa ccgatctcct cgatgcgggc aaggatggct tcgccttcgg
    18061 agcgaagaat ttcggggtct tccaatccct cgaggcgctt gaatgtctcc tggcaacttc
    18121 gtttgaggtg ttcgcagttg cttgcctggc ggggatcatc gaccgggata tagaaatccc
    18181 tggtctggag ttttttaagc tctagcttgt gaataatcgc gaggatttca tccttgctga
    18241 aagtctcttc gtgatgtatc ccgcatttcc tgtctatgct cgcaacccag tgatgcataa
    18301 ggatatgagt catctgagct tctgtttgct gtccatcttt atacatctct gtaaccagga
    18361 atactcctcc cggcttgagg acgcgcagca gttcggcaaa gaccttgtca cggtgctgaa
    18421 ggtgatgcag cgaatttgag atgcagacca gatcaaagta cccatcctca aaacgcagct
    18481 cttccaattc catgcgatag atctccacgt cgttgtccgg aaagaacttc tgagcgtagt
    18541 tcacactctt gtcattggaa tcgacaccaa tgatctggac ataagctccc agcttttgcc
    18601 tgagcacatt gataaactca ccgcgccccg tagcggcatc aagcacgtgg gaaacttgga
    18661 attgattgag gatgtcaaaa acatctttca ttacgctgaa cctcgcttgc gtaggtgatt
    18721 gggatgttag gtccttaaag gaatccatgc gttctcatca taatcgccaa accctcagtc
    18781 tggccgacgg tgctattctt ttgtcgatgc tctttttcgt caagtctttt tcatgaacgt
    18841 ccctcgtttt caaaaaatca cttgccataa tgcggccatt attaagtagt gcaccccaac
    18901 agccctgtat gaatgaataa ccaattgcat aagggcgata agcatggaga tgtgtcttat
    18961 gatcgaagta ttggatctgg tcaaagagtt tcccaaaaag gacggcaccc ggtttaaagc
    19021 tgtggatggc gtcagcttca acgccgggga tggagagatt gtctgcctcc tgggtgtgaa
    19081 cggagcggga aagaccacta ccatgagggt gctttccacc gtttttaagc cctctgcggg
    19141 gacggcccgc attcagggtt acgacatcct cacacaggca gacgaagtgc gccgccatct
    19201 gggttttctt tccggcgata ccggacttta caaccgtctg cgcgccagag agtttatcac
    19261 ctatttcggc cgcctcaacg gcatcggtga ccctgccatc aaacggcgga tggatgagat
    19321 ggctgatctg ctggatatga aagattttat ggacaagaag atcgagcttc tctccagcgg
    19381 catgaaacag aaggtctcga tcgtgcgctc cattatccac gatccccagg tgatgatttt
    19441 tgatgagccc accagcggtt tggatattct cacagcccgc aacatcgtct ccttcatccg
    19501 ggattgcaag atcaagggca agtgtgtgct cttttccacc cacataatgc gcgaagcgga
    19561 gcgcctcgca gacagagtgg taatgattca tcgcggcaaa gtgctggcaa acgggaccct
    19621 ggaagaattc cgcgcccaaa cgggccaaac cgatctcgat gacattttcg tgcattttgt
    19681 aaatcagtcc agctttgagg aggagcttca ggcccatgag ttttaataaa gctttgatcg
    19741 tctatcgtaa agaactgctg gaaatgatgc gggaccgccg cactctcttt gccaccttca
    19801 tcattccgct gattttgtat cctcttctgt ttataggttt tggctctatc atgagccgtc
    19861 agacggccgt tctggaagaa cgcggcgctg agatcgcttt tgccgacagc gtaaacaacc
    19921 acatttcggc tgccatcatc gctggcatca aaaccaataa aggctataat ctactgccat
    19981 acaaccagga cacccagggt ctttataaca gcggtaacat ccaggcgatc gtaagtatcg
    20041 gtgattctat caccgcggct ggttcatccg tctataaggt cctggtaagc tacgactcgt
    20101 ccaaagagcg caaccaattg atctacagaa agatcaagga ttcggtcacc gagacgaaag
    20161 aaagctctat tgccaccgaa ctatccaaaa gggatgtgga ccccgccctc atggaactga
    20221 tcagagtgga cgccatcgat acgtcaagcg cgcaaaaacg cctgggctcc tatctgggca
    20281 tgatcctgcc gtatatcatg atcatgatgc tggtggccgg ggctgcgacc gttgctggcg
    20341 accttgtggc gggagagaag gagcggcgca ctctcgagac cttgctggtg tcttccgctc
    20401 agcgcaacga actggtattg ggcaagtatc tcaccgtcat cacggtagct atggtcaatg
    20461 tggtggtaaa cctcttcagt atgggatttt ccatgcagtt cttggtgggc agtcaaggca
    20521 tcgccagcag catgacacag atgcccgtgg tgggatttct gattctgctg gcagcgatgt
    20581 tgcccttggc tacattgttc gcggcactgc ttatgtccat ctccacattt tcgcgcaacc
    20641 tcaaggaagc cgcttcctat cagcagccgg tgatgatgat ctcgatgctg cttggtatga
    20701 tctcgttcat cccctcggtg gagatcaaca acctgctggc cctggtgccg gtcgtgaata
    20761 tcgccttgct ctttaaagct gtgctgatca atgaatacca gctctcgcac ctgctgatca
    20821 cggtggggtc caccctgctg ctcgatattg gagctatctg gcttacagtc aagctcttca
    20881 gcacagaagc gatgctgttt cgcacggacg aagacagttc cctcaaaaac gtccgcaaag
    20941 agaagcgcaa tctctttaac cccttcaacg gcatggtgtt ttttgctctg gccctagtgg
    21001 ttatgtacta cctgggcagc tacttccagc gtgaagatat gatgaagggg ctggtgcaga
    21061 cccagatcct gattatcctc ctgccccctc tgatcatcct gaatatcttc aagctaaaac
    21121 ccaaagagat cttaaggata acgttgccca aaccccggga gctggcgatc gtacctttca
    21181 tcgccgtttc gggagcagtg ctggtgactc ttctgatgaa tgttgtggat gccatctatc
    21241 ccttcccgga tcaatacacc gagcagctga tggggctttt taagatgtcg gataatcttt
    21301 gggtgctgtt gggaatagtc gctttagctc ccggcatctg tgaagaaatc atgttccggg
    21361 gctttttgat gcgctttttt gaaggaaact ccaaaaagat ggctgtcgtg atcagcgctc
    21421 tgctctttgg agtctttcat cttgatccct tcaggcttat ccccacattc ctgctgggac
    21481 tcattctcgg ctacttaacg atccgctccg gaagcattat caacagcatg ctgtatcaca
    21541 ctctcaacaa ttccgtggcc gttctggtga ccacctatgc gtcgagcgct tggatgaaac
    21601 cgctgttgcg aggcagtgaa gggctgcagt ggtggttggc cgctccggcg gcgctgatcc
    21661 tggcgggttc gctttggcta ttccataaag tcactacccc aaaaggagct tgataatgtg
    21721 cggaatagta ggatacatcg gttctcgcaa cgcgctgccc atagtgatcg aagcgctcaa
    21781 aaggctggaa tacagaggat atgacagctc tggctgcgct ctgatccatg aacaggatat
    21841 gcaggtctat aagaagcaag gcaagatcat cgagctggaa cgctcacttc ctgagcccaa
    21901 caaatgctcc ggtacgattg cgatcgctca tacccgctgg gctactcatg gtgagcccac
    21961 ggagttgaat gcccatcccc acctcgattg cgaaggcaag ctggccatag tccacaacgg
    22021 catcatcgaa aactatcagc ttctcaaaca acggcttaaa gaacttggac acaaattcta
    22081 cagcgataca gacacggagg tgatcgtcca cctcatcgag caatacatga gcagcaccag
    22141 ctgcctggaa gatgccgttc gcgaagccat gaaaagggtg gaaggcacct atggcctggt
    22201 cgtcctgtcc aaagataatc ctgacaagct gatcgcagtt cggaagggat ctcccatcat
    22261 aatcggcatc ggtgatcacg aacactttat tgccagcgac gtcagcgcga tcgtgatcca
    22321 tacacagaga gtcatatatc tgcaggacaa cgagctctgc gtgatccaca gagatagttt
    22381 tgagatcact actttggata aagtgcaggt agaacccaag atatccctgg ttgactgggc
    22441 catctctgcc atcgaaaagg gagatttcaa gcactttatg cttaaagaga tctttgaaca
    22501 acccctcacg atcggcaacg gtttcagagg ccgcatcaac gatcagcttg cttcagcccg
    22561 tctgggtgga ttgaatctgc cctccccgga gctgcggaaa gtcaaacaga tccacctgat
    22621 cgcctgcggc acttcttttc acgccgctct catagggaaa tacatcattg aggacatggc
    22681 gcgcatccct gtccatgctg aatacgcctc cgaatacaga taccggaatc ccattatccc
    22741 ggatgacacg ctggttttcg tgatcagtca atctggtgag acagcggaca cgcttgccgc
    22801 tctcagagag gctaaagcca agggagccat cgtcctggga atcaccaatg tggtgggcag
    22861 tacgattgcc cgtgaaagcg acggcgggtc ctatatccat gccggaagcg agatcggagt
    22921 ggcttccaca aaagccttta cctcccaggt aacgattctg acgctgctgg ctatcctgct
    22981 gggacgccag cgttccctct ctccgatgga cggacagaaa tacatcaacg agctggaaca
    23041 aatccccgct aagatcgaga gtattctggc gcaaaacgat atgatccggg agatcgccgc
    23101 atccatcaaa gacagcacca acgcgctcta tctgggtcgg ggcgtgaatt atcctgtggc
    23161 cctcgaaggt gccctcaaac tcaaagagat atcatacatt cacgcggaag gttatccctc
    23221 cgcggagatg aaacatggcc ccatcgccct tatcgatgaa cacatgcctg tggtggcgat
    23281 cgcgacccac gacagtctct ttgataaagt ctattccaat ttgcaggaag tgcgtgccag
    23341 aaaagcccgc ctgatcacca tcgccaacgc tgggagcgaa gaactggata aaatctccga
    23401 gcatgtgatc cgtatcccgg aaacgctttc caatcttcaa ccgctgctca ccgtgattcc
    23461 tttacagcta ttggcctatc atgtggctga tctgcgaggt tttgatgtgg accaacccag
    23521 aaatctggct aagagcgtga ctgtggagta gatagaacgc agattagctt atttaacaaa
    23581 gagttaaaaa gaatagaggg atgcatcaac gctccctctt ttcttttgtg caacctgttc
    23641 ccttattgct ataagcgggt catcactgga ccaaaaccgt cccaagcctc ataaatggct
    23701 tttgggtccg gcttgctcat gatgctggta acctcagtta ataagggggg aggaactgag
    23761 atcagtaaga aagcctatca acctcagtcg ttataacttc actccgcaag ctgcttcacc
    23821 gcctcagatc ccaggtccat aaacacttcc gcgacggtgg gatgggcgtg caccgctttg
    23881 gcaaggtcat atgtggtgcc ttccatttcc atcaggatgg cggcttcggc gatcatatcg
    23941 gtagcgttgg cggctacgat ctggactccc agcacctgcg aatactttgg ctcgtagagc
    24001 aagcgcagaa agccttccga agcgccttcc gcgagggctt tgccgttggc ggacagcgaa
    24061 tattcggcgc tcttgtactc gattccggct tgtttgagct ggagttccgt ctgccctatc
    24121 tgcgcgattt cgggatcgct gtagatgttt atgggccagg atgtgtctgg gacttcttca
    24181 gttgccttgc catggatgaa gttaacgata gccagcgcct gagcggaagc cgcgtgagcc
    24241 agatagctgc ggccgttcac gtctccggct gcgaagatgt ttggcacgga actgcgatag
    24301 tattcatccg tcaggatgta tccgtcttcc aacgcgagct tgatttgcat ggatggcagc
    24361 accgcctgac gccaggagca attgatcacg gcatcctggg cgatctcctc gtcttggtaa
    24421 taagctttat ttgcaacgaa tttgatctca gccgcgggaa caatcctcag cttttccttt
    24481 ttaagcttct tatccacata gctttggata tagggatcga gacctggaag caggatatcg
    24541 tgggtgagag ccaggatggg atccttgcct aacagcctga aaaactgtag catctcatag
    24601 gcgatggggc cgtgtcccca aatgaggggc tggtgtggaa gcgcttccag ctcggagaga
    24661 cgctccagac ctatcagatt gggcacgcca tcaagctgag cgggacgcga accggtggca
    24721 atcatgatgt gttgagtttc aaagacgcgg ttatccgcct ccacctgatt gggagacaat
    24781 atctttgcct cggctttgag aaactccacc ccgttctttt tccacagata ctcgatcccg
    24841 cgggttagct ttgccaccac cgcggaagaa cgctttttag cctgaagcca gttgaagctg
    24901 agcttatcgg cttcgattcc gtcgatgcca aactcagcgg cctggcggga aatcttgtga
    24961 tagaggcggg cgctttccag ccacgttttg gaggggatgc agccccagtt gaggcacatg
    25021 ccgcccacat agcgtttatc gatcacgagg gtcttcagcc ccgtctggcc tgctctaatg
    25081 gctcccacat agccagccgg accgcttcct atgacaatga ggtcatactg catggttagc
    25141 tcctttgtcg ctgggggcga cagacagcat tagaaggggg tctttgagat attccaacac
    25201 cagattgagg aagcgggcca cctctccccc atccacgata cgatgatcta cggacatgga
    25261 gatgggcaat acgtttccaa tggcgatctg gccatctttg atcaccggtt tctgggtaag
    25321 tctgccgatt ccaaaaatag ctgcctgggg ataattgatc acaggcacgg caaagtaacc
    25381 gcctatggaa ccgtagcttg tgatgctgaa ggtgccgcct ttgagctcgt caagacttag
    25441 ctggcgctta ggggccctca ccgagaaatc gttgatcctg gctgtcagtt cctgaatgct
    25501 gagttgatcg gcgtctcgaa tcacaggcac caccagtccg tcgtcggtat ccaccgcgat
    25561 cccgatgttg atgtgatctt tatggatgat ccgctcgttt tccagatcga gctcggcatt
    25621 gaggatggga tgctctctga gagagagtga tactgccttg atgataaatg gcagatagct
    25681 gagtttgagg cccatcgctt caaagcttgg tttgagacgc tttctggctt ccacaagttc
    25741 tgtgacttcc acttcatcca tcacgctcat atgaacggcg ttttg
//
LOCUS       DAOSFN010000015        24853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1583_length_24853_cov_8.646060, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000015 DAOSFN010000000
VERSION     DAOSFN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 24853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24853
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1583_length_24853_cov_8.646060"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1196
                     /locus_tag="PLO35_02120"
     CDS             105..1196
                     /locus_tag="PLO35_02120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH78487.1"
                     /translation="MVQTAFCVLTPLYVLIPILAALAVLLCRKGKITTAQGISVIVAA
                     RNEEHNLPVLLSSLAKLDYPPEAYELIIVSDHSTDASVDILRQWQNRLNLKVIDWQEE
                     EEGIIGKKAALLQGIRAAKHELLAFTDADCEVPSTWLRAFSQAFENDVDYVLGYSLIK
                     RDKQDGYFRLKNFERGIYYSLAALGMRARHPITSSACNMAYRKSAFLASKGFEGIGHL
                     ASGDDDLLLMKMMPRLRRAVYNPSPAQRVVSYEGHDLSKRHHTNVRRASKFRYFPLWL
                     QLMGVFVFLYFILFYLSIAALALGSSNPWLPLMFMIKTGTELSLGFIHFFRTGLPWLG
                     ALYPVQLLWFPGQFVYYSLRGSLGKYRWK"
     gene            complement(1197..1445)
                     /locus_tag="PLO35_02125"
     CDS             complement(1197..1445)
                     /locus_tag="PLO35_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78488.1"
                     /translation="MTYKVILVKIDHRSTEAARVQQILTEYGCNIKVRLGLHEVSKEF
                     CANDGLVILEVEGSQEELKQIIDSLNQVNYVQAQLIEM"
     gene            1753..2955
                     /gene="tyrS"
                     /locus_tag="PLO35_02130"
     CDS             1753..2955
                     /gene="tyrS"
                     /locus_tag="PLO35_02130"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOH78489.1"
                     /translation="MRFEKELKVIQRAVDEIVPLEDLVAKLDKSQKTGTPLRIKFGID
                     PTGYDVHIGHLVPIRKMRDFQDLGHIGVIIIGDFTAQIGDPTGRDESRPPLTHEQILY
                     NSEKYMEQLYTVLKPELTEVRYQSEWFGPMGMTDVLKLLGKFTLAQFMAHDTFRNRYE
                     SGLSLGMHELMYPVLQGYDSVAINSDVELGATEQKFNILCGRDMQRHFGQEQQVAVLS
                     PILTGTDGVNKMGKSLNNYIAVFDPPAEKYGKVMSIPDSLLINYFKYAAYSDTDALEA
                     IKRELEDGVNPMLLKKRLAREIVGFYHGEEAALQAQEGFEKQFSQKEVPEDLQEYVLP
                     AGPNKVSKILVDCGLCASSGEAKRLIQGGGVSVDGNKINAWDAEIEISDEAILKAGKR
                     RFVKLVKS"
     gene            2955..3611
                     /locus_tag="PLO35_02135"
     CDS             2955..3611
                     /locus_tag="PLO35_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOH78490.1"
                     /translation="MGYEFQLKLIKGAVTAIIIGVIFYCIILHEISHAVVARWLGDDT
                     ASRRGRLSLNPLKHIDWFGTVILPLLLYFSAGFIWGYAKPVPINPYNFRNYKRDMGLS
                     ALAGPVTNILIGLFFAVLFHLATGFSLIQQVCYFIVFFNFLLAFFNLIPIPPLDGSKV
                     LGMALTDEAYMRWTAQERTGMIALFAIIMVSNLLGLNIFGKLVMPPVQGLMALLGMGT
                     "
     gene            3670..5091
                     /gene="glnA"
                     /locus_tag="PLO35_02140"
     CDS             3670..5091
                     /gene="glnA"
                     /locus_tag="PLO35_02140"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOH78491.1"
                     /translation="MDIQKLKQMIEQHEVQAVDLKYCGLDGRWYHITFPARRLEKVLV
                     SGIPFDGSSIPGMRSVESGDMVLMPDTDTAHLDPFYETPTLRMICSICDAESRVGVKK
                     DPRSVAQRAHDYLLSTGIADNSTWIPELEFHLFDSVEYNSDSFSSGYTVTSCESKDCL
                     PDDFDDLDGVARQDVKGYHADTPFDRYYEIRQEIVEAIEAQDIKVRYHHHEVGLSSQQ
                     EIETELLAFPKICDDTMVMKDIIRRVALENGLSATFMPKPVYNQAGNGMHFHIMLHKK
                     GKNVFYKKGGYADLSNEAIWFIGGVLKHGRSLVAFTNPSTNSYKRLLPGFEAPVKLFY
                     GLANRSAAIRIPRYANTPESKRFEFRTGDGTCNPYFAMSAILMAGLDGIKNKIDPAKY
                     NLGPFDDNVYAWPEKKKAKLLSIPADLREAMLCLEKDHDYLLAGNVFNEELIESHIAM
                     KRVEHDAVSNRVHPHELMLYYSL"
     gene            5157..10625
                     /locus_tag="PLO35_02145"
     CDS             5157..10625
                     /locus_tag="PLO35_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH78492.1"
                     /translation="MKKTVLLLVVGLWISLCLAASPEGTPVSYKQAFSITAQSDNHMD
                     IRFSLPDFELETVNVGDQVFDRIIMPDAGTTLESGLPELPTVSISLAIPRQGSTQASL
                     MGVEPELINSFLAYPVQQGQELESPKSFVYNAGYYSQGAAYPQNVVQISEPYILRDFR
                     LVTIQVNPFIYNPLTQALTVNQTIDLRITFTQEPGLNELAGEFSNYSLAFEKIYEASI
                     LNFDDYRYQMFSRTPPRYLLIHGNNTDPNFLNAINDFVLWKRQKGADVDVATTAAGSA
                     GTTTTTIKNYIQTAYNNVALRPDYVILVGDTQGSYTIPTFSVSSGSGDYPYTHLEGTD
                     QVGDVFIGRISAENLSQLQTLFAKIYLYERDLNINTADWLNRMLLVGDWSPSGISTVY
                     ISKYIKERALYINPSYTFTELYGSDPSPSSMNTAINQGIGFFAYRGYINMSGWSPSES
                     SLNNAFRLPHAIIPTCGTGNFGSSSPGTTEAFIRLGTAAQPKGAVTAIGMATSSTHTV
                     FNNVLHGGIWGGLLTYGMRTMGEGLLNAKAYIHQMFGVSSPSNVTSFAHWFNLMGDPT
                     MEVYFGIPSTFNVTTQTTIPLGLSLLDVAVRDNNMTIVADASVTLSQGTSILSRGYTD
                     AEGNVILILPSGMGTGNCVMTVSKHNFKPVQIPITVDTSGTLVPGSIVIDDDTTAPSQ
                     GNTDGFANGGETLEILFGLQNTSAATISGISGYITTTSPYATFSDSLVTYAAIPAGQT
                     GFNASPIVLHVAPNTPHNSMLRLHIILTDSMGNTYDISEFMPVTNANIRYHSYALINA
                     GTNNALDPGETAGFTVTLSNIGAIGVTGITARLFSDNDLVSVTNHTIEYGDMLQNVQV
                     TPSVQTFELLARTQVLPGMLIPMRMKLYNAAGFEQWLAFTFTVGVVTVNDPLGPDSYG
                     YVIYDDTDTAYPECPVYDWVGIAPAEGGVGTSLNISDAYTSGDEGDQVGNNPLAVVNL
                     PFPFQFYGISYSQITVASNGFLVFGTTESAEFRNYRLPGPMGPNPMIAPFWDDLATHT
                     GSGIYTWFDRNNHAFVIEWYNLRNGVNGSSPETFQVILYDPAVHAGSLGDGPIKIQYH
                     TFNNVDATSSTSHHGCYSTIGIEDHSGIVGLEYSYSNTYPTAASPLGNGRAIYITNIP
                     IYYEASHLVLGETYITDPNGNGVCEPGELIELGVQLSNIGNAVANNPTATLSTDSEFI
                     TMINAETTYFPIAGEGFGVNRTPFRFTVSPDCPNGTVVNFSILIVSGEDSWSRQFSLR
                     VDASQLEYLGFMINDADTNYNGVIEPQESVKLVVNVRNHASVAASDLMATLSNADPAI
                     VIDEPIITKPAIDANDIMQFVFEIFNGGTSTQSEYKGFQFNLTAGNGLPLSATMMVPF
                     NMNNIFSDFETENGNFDSETGWAWGAPSQVTPYSGTKLWASNLSGNYPDLVTYNLHTP
                     IYALQTGSVLSFMHRYGMENNYDGGNISISTDAGNTWTLLTPTGGYSHNSLAGLAGQP
                     GFSGMLANWQPVTINLNQYSNQMAMFRFRMGSDGGTGGIGWFIDNFELSNVNQKTGYL
                     YGVVIPTSTTSPTEAMVKAGNFYTTNPDQEGNFKLFLPNGTHSVTATLDYHQSSSIHN
                     IVIGPNDPTHYTEFTLINLPKPVGIDFDVDNDSGLMSLTWNEPYDPVLPVMAYKVYKR
                     FNTGPFEFVQETTSTNYSETLELEGDYKYYVTVRYLNTDGTPSDLVAFSYPFVDSDDN
                     NNPGLVTKLNHNYPNPFNPSTTIAFDLAKAGNVKLSVYNVKGQLVRRLTHANLPGGSH
                     RVTWNGVDESNRPVASGIYYYRLETRDYTKTNKMLLMK"
     gene            complement(11003..12130)
                     /locus_tag="PLO35_02150"
     CDS             complement(11003..12130)
                     /locus_tag="PLO35_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH78493.1"
                     /translation="MNYKTMPGSQDKLSALGFGCMRFPMDDQGKIDQPRAIEMLQYAY
                     DHGVNYFDTAWPYHNSESEPLLGEFLASIAREKVFVATKLPCWLVKEAGDPLKYLKLQ
                     LEKLKTGYIDYYLLHALNQRSWAAMKKLKVLDSLVQAKKDGLIRHIGFSFHDSYPVFK
                     RIVDGFDWEFCQIMLNYLDTHYQAGMNGYRLAVSKGMGVISMEPLRGGKLVAPIPEEV
                     EKLWQGSEFYQKPLERALAWVWDLPGCSVCLSGMSSLEQLKQNILLEKHLSNKTLTDK
                     ERNLYIKARRAYIKRIAIPCTECRYCMPCPHGVGIPFVFGQYNEAMMFGNKERHKQEY
                     EAFISPEMRADKCTHCGECLSKCPRHIAIPDEMDRIRDYFS"
     gene            complement(12375..12791)
                     /locus_tag="PLO35_02155"
     CDS             complement(12375..12791)
                     /locus_tag="PLO35_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOH78494.1"
                     /translation="MISYRKELSFKTARRREYINITAQVQNAVTESRVKEGLCLVNSM
                     HITSSIFINDDEGGLHADFEVWLERLAPEKPHGQYRHNGFEDNADAHLKRQIMGREVV
                     VAITDGKLDLGTWERVFYGEYDGMRHKRVLIKIIGE"
     gene            complement(12791..13492)
                     /locus_tag="PLO35_02160"
     CDS             complement(12791..13492)
                     /locus_tag="PLO35_02160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH78495.1"
                     /translation="MSSKFKIKAIIFDLDGTLLDSLRDIAESMNQALAESGLPEHPVE
                     DYRGFVGNGLTELTSSVSPEDKRGEKEVYALAEKFWYKYDESWFLHTKPYPGILYLVQ
                     LCVARKIKVAILSNKVHYFTKKMIRHYFRGVMINHGKNPFGIYSGEQPDMPLKPDPTR
                     ALELADRLKVKPENIALLGDCPVDVQTAKNAGMISIGAAWGFCSRKELEEAGADLVFD
                     QPTDLSKYLEKAPIV"
     gene            complement(13494..14348)
                     /locus_tag="PLO35_02165"
     CDS             complement(13494..14348)
                     /locus_tag="PLO35_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOH78496.1"
                     /translation="MFSDFDPAALVRQFITPERVSLGIRVILILAIGIPLIKLIRKLV
                     QRLVRDKLSAQSEQLIIRTVYYVSILILTVSVLNEFGFKLSALLGAAGVFGVAIGFAS
                     QTSVSNIISGIFLISEKPFVIGDVVQVGSTIGTISSIDLLSIKINTPDNRFVRVPNET
                     MIKTEVINITRFPVRRVNLDISVSYNDDLKQVLEILMDIAQNSQIALKDMEPLTSIQR
                     FGASGIDILYGVWGNTSDYFALQNELIISIKERFDAAGIEIPYPHLSLYSGSHSDPIK
                     VNTLNKEG"
     gene            14514..15554
                     /gene="wecB"
                     /locus_tag="PLO35_02170"
     CDS             14514..15554
                     /gene="wecB"
                     /locus_tag="PLO35_02170"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOH78497.1"
                     /translation="MKIIQLVGARPQFVKLAPISQLIRQSHKELIIHSGQHYDYLMNE
                     VFFKQMGIPEPDYYLNIGSGSHGVQTARILEALEPVLITEKPDWLLVYGDTNTTLAGA
                     LASSKLGIRTAHVEACLRSFNKAMPEEINRIVADHLSDLLLVPTPTGMTNAKSEGLLD
                     KARLVGDVMTDSLRMGLEAAQASGVSSSQASPYYLLTLHRPYNVDDPQQLVHILKSLN
                     TLQQTILFPVHPRTSRLLDAQTRAQFEFIRFLEPLGYLEFLVHMSGAEMVLTDSGGIQ
                     KEAYILKKPCVTLRTETEWLETVESGWNLLLPPQDETFPSAIRTFCPPQEHPSLFGEN
                     VSREILNLLVSS"
     assembly_gap    15690..15789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15795..16421)
                     /locus_tag="PLO35_02175"
     CDS             complement(15795..16421)
                     /locus_tag="PLO35_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH78498.1"
                     /translation="MLRHLIPTLTASLSGFLLAFLLGIFTALAMHASTLIKRLVYPYL
                     IVSQTVPIIAVAPLLILWFGFGITAKVFTVVLVSFFPIALGFYDGLRQVPLEQVLLLR
                     SMGAGTWTIYRLLRIPASLPSLFTGLKLAATYSVMGAVIGEWLGGNAGLGIYMTRATK
                     SFQTAHVFAVILIIVALSMALFGIVALLDRILLAWHYKKQAEYEDPAL"
     gene            complement(16577..17548)
                     /gene="murB"
                     /locus_tag="PLO35_02180"
     CDS             complement(16577..17548)
                     /gene="murB"
                     /locus_tag="PLO35_02180"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOH78499.1"
                     /translation="MGHPAHDNRQEAITLKDLIKQHLPELLDSGALLFDEPMKAHTSF
                     NIGGPADLFCLPSSQAQIIQVLRFAQENSLPWMILGKGSNLLVSDKGIRGIVISTQKY
                     TKLSLDENYVSAFCGVSLRELCEFCRDRGLAGLEFASGIPGSVGGAVFMNAGAYGGEI
                     KDALYCSKALEPTPQGLTAPHPILHLKHADHQFDYRTSSLQTRGLVHLSSVFKLRYDS
                     PEEINARMLELDSQRWEKQPMELPSAGSVFKRPEGHFTGKLIDDCGLRGFQIGGARIS
                     DKHCGFIVNVDNASAADVDALIKHAQTVVQQRFGVSLRTEIRKVGEW"
     gene            complement(17587..20469)
                     /locus_tag="PLO35_02185"
     CDS             complement(17587..20469)
                     /locus_tag="PLO35_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOH78500.1"
                     /translation="MTAIPPDIKTSPGEEFLRTLDFVALDIETTGLDFQNCEIIEIAA
                     VRFTGSVIVDRFQSFVKPKHGIPKYIQHLTHIDPIDLKAAPQIAAVLQKLVVFLGESV
                     ILGHNVRFDTGFIDHNLALSGHLPLTNEKWDTAELGRIYLPYTNDHTLGTMCRHFDIE
                     LVNAHRAEADAEATGKVLISLARFITEHYSMMTNARLLDLSRQSQADESSHEFLRRLV
                     EWQRRHLGAAKLPLPENPWVNVIDHTVEKQTIPGMEQVFGPNGILEHRFENFEFREGQ
                     LQMGEAVKKAFAGKKHLAVEAGTGVGKSFAYLVPAMEFANKEKSKVVVSTNTKNLQEQ
                     LFFKDLPRLKDLIPLSFKAVLVKGRENYVCQRRWDEIMMEQTRGLSSWEARGLMYLYI
                     WKLQTLSGDVSENSSFDRGRYSLLWRKINSDRFSCMGKKCPKVNECFVMKLRKHIETA
                     SIVVANHSLLLADLRNEFSTLGEYSHLVVDEAHNLAASASGLLGFELSYAELANSLFQ
                     LSGSGKKRSGLLHQIELNVSKSAITDAAKNQATLLIDKLGNTVEDLRTLNTRLFQEAA
                     TRVNLAESFGKLRIKELVGFDVLYRHLGNLINAWKELMKDLKALGNVYSSFNSELIHG
                     WEETVETIQSTYSRNSETEGKLLSLQTPDLENYALWLQNDPRPDRNTPGSSFCYAPVE
                     VDRILYELLYSKISCIVFTSATLALRGSFKYFFNQSGLHLVDSDKIVTEIVSSPFDYD
                     SQSKLMVSSFLPEHKDKFFLNQALGCVEQILASTEVGTMMLFTSYRDLNSVYDHIGDT
                     LYHRGRPFFAQGKIGSRSSILQEFKNHKNAVLLGTSSFWEGVDVQGESLSMLILFKLP
                     FLVPSEPIVEAYIDKLERDGKDSFMHYMLPNALLRLRQGFGRLIRSKTDRGIVLIMDS
                     RVSTKRYGSYFKEVLPTHCLELRSELELINEVTSFFNRKPHVEHDA"
     gene            complement(20469..24743)
                     /locus_tag="PLO35_02190"
     CDS             complement(20469..24743)
                     /locus_tag="PLO35_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOH78501.1"
                     /translation="MPERLYRIMHQFVSERFLSSRVLELLKNSSASHLIELSGRAGSG
                     KSYLISPLVEQLKVKYERVRVFSPNPMSFNQFEEIVELVTDLETQQLKELIQEHQSRY
                     RTGRKHDFFYFLTSRLNELEMLKPMVLIVDDCDVLDSFSREFLQYLVHYAEEAGIQII
                     AISQNRLFPFAVFEYIPSLTTDDLQQILQVAFPNEQLGYVSGSEILQRISDGNLMVVE
                     RILNEMLVKDTGFDLSPFLERHFDPTQLFFGSLDRLAVSQRDLLISMIVFNNDVDAAV
                     AKGLGISKNLTNDTEGLIREDLLGRIESSYAVQKKSSLMRWIDEHPEQFSPELKQRVI
                     KVLKGRSGCEQVLVRLMMMSNIYDPELFSRLTAYLESISDESGAAELWRYILRHVTEP
                     RELLEAQKHIGIATAGMNDKDAAVDSFRAALQVCTENSLPAEEIVWHLASNLFAQNSS
                     SFALEIIKKYTPASIDEYWKIKILLLKADILAEGEEFDKALEVLENVLHSLSKVEDKI
                     KRYQVQAEAKKTKGKIHYYINEWDQSEEAFKDAETMYNLASDHVGLAAIYNNLGVLYM
                     FQGDWERSEQFFLRSLTLEKEHFNLNGISVCYNNLGGLMDDKGDASRSLFYLEEALKL
                     QKMLNEPYNITNIYNNIGVTLMDHGDYSKAEDALKRSLETAVEFGFYRNTVASLNNLG
                     ALYFKMGEWNRSIELYEQAILKSQDNNFSEGLLRSFNNLGEVYEKQGELNLAYDLYFK
                     GLELLPSVSDEYIKAELFGNLGSVLTRLQKFKEAYSYLVESLDFFKSLNARDKIIEGY
                     QKQAFYFILSHNYESGDYYITQALKMATDQNRSFEVGRAHYLRALLERKNLEQAREHL
                     DEAIRHFVETDNDYELSLANYELASVLFDLQDWEQALQILKNNKKIIQRYGSIKLLEQ
                     NDILSQKIAREYSAQMQDVKFEENLLNQFYEITQKLNTISDLDLLIEQSLSSLVEISE
                     ADGGILCLHTGKQMPDHWDYKIYRNFSPDDKLHETFLGICTDVYRDNKAADLKQPHFA
                     PSYNNIICIPLSIRKSVLGVVLLFCQGGSHYFSERIVNLLNALSNQVIVIIENVRSAN
                     LEKTHAIIREQLNAGNLYANIIGKSPEMERIFEVIEKVKDTPTSVLLEGPSGTGKELI
                     ARALHYSSNRRGKAFVAQYCGALPETLLESELFGHVKGSFTGAAYDKKGLFEIADGGT
                     FFLDEIADISQSTQAKLLRFLQEGEIKRVGATKTEKVNVRVVCATNVSLIDKVKEGDF
                     RLDLYYRLNVIRIEVPPLKRRQGDIPLLAIHFLDKYTKRIGKEVPGITDEAMKILEQY
                     DWPGNVRQLENEIERAVTLAEPGSFIKPSDFSDEVRRFMENIRTISMLSGKKNLKDAI
                     EELERKMIYDCMEGCGWNQTQAAKELGLSRQGLIKKLSRYNLNREE"
BASE COUNT         5919 a         6653 c         6159 g         6022 t
ORIGIN      
        1 atcgagatca aacggcgcaa catcaacgag gtcaaggaaa aagatccgct tctggccaga
       61 tacatagagg tcaccgattc taaattggga gcgggatact gagcttggtc caaaccgcgt
      121 tttgcgtcct gacccccctc tacgtcctga tccccatcct ggcggctttg gctgtgctcc
      181 tctgccgcaa gggaaaaatc acgacggcac agggcatcag cgtgatcgtg gcagccagaa
      241 acgaagaaca caacctgcct gtcctgctaa gttcgttggc gaagctcgat tatcctcctg
      301 aagcttatga gcttatcatc gtcagcgatc attccaccga tgccagcgtg gacatcctca
      361 ggcagtggca gaaccgcttg aatctcaaag tgatagactg gcaggaggaa gaagagggca
      421 tcatcggcaa gaaagccgct ctgctgcagg gaatacgagc cgccaaacac gaactgctgg
      481 ccttcacgga tgccgactgc gaagtaccct ccacctggtt gcgtgccttc tctcaggcct
      541 ttgaaaacga tgtggattac gtgctgggct attctctgat caaacgggac aagcaagatg
      601 gatatttccg gttaaaaaac tttgagcggg gaatctacta cagtctggcc gcgttgggca
      661 tgcgggcccg ccatcccatc accagcagcg cttgcaacat ggcctaccgc aaaagcgcgt
      721 tcctggcatc aaagggattt gaggggatcg gacatctggc ctcgggagac gacgacctgc
      781 tgctgatgaa gatgatgccc cggttgcggc gcgccgttta caacccaagc cccgcccaga
      841 gagtggtttc ctatgaaggc catgatctgt ccaagcgtca tcacaccaac gtccggagag
      901 cttccaaatt ccgttatttc cctctctggc ttcagcttat gggtgtattt gtgtttcttt
      961 actttatact attttatcta tccattgcgg cactggcact tggatcaagc aatccttggc
     1021 ttccgttgat gttcatgata aaaacgggca ccgagctaag ccttggtttc atccactttt
     1081 tcaggactgg cctgccttgg ctcggggccc tgtatcccgt tcaactgctg tggtttccgg
     1141 ggcagtttgt gtattattcc ctgcggggca gcttggggaa ataccgctgg aaatagctac
     1201 atttcgataa gctgagcctg gacgtaattc acttggttaa ggctgtcgat gatctgcttc
     1261 agctcttcct ggctgccttc cacttccagg atcactaaac cgtcgttggc acagaattct
     1321 ttggaaactt cgtgtaatcc caggcgcact ttgatgttgc agccgtactc cgtgaggatt
     1381 tgctggactc tggcagcttc ggtgctgcgg tggtcgatct tgactaaaat cactttgtaa
     1441 gtcataactt acctcctgaa acccaaattc gccgcgctgg aaaacttgtc cagagatttt
     1501 ccgcggatct ggcggcagcg ttgatctggt cgtgttccca agcctcacgg ctcacttgag
     1561 cacgataatc ctgatgcaag cctgccgaat ccatcatttc tatcacttca acaattccgg
     1621 cctctaacat ccaccgcgac cgcgccgaag agctaagaag gacccacagg catgagatta
     1681 gcttgacaaa agcagggtgc caggatttgc tgtttttttc ttgtaacttc agataaaaat
     1741 caggataaag aaatgcgttt tgaaaaagaa ctaaaggtga tccaaagggc cgtggacgaa
     1801 atcgtcccat tggaagactt ggtcgccaaa ctggataaaa gccaaaagac tggcacgccg
     1861 ctgcggatca aatttggcat cgatcccacc ggctatgacg tgcatatcgg tcatctggta
     1921 ccgatccgca agatgagaga ttttcaggac ctcgggcata tcggggtgat tatcatcggc
     1981 gattttaccg cccagatagg tgatcccacc gggcgcgacg aatcccgtcc tcctctcact
     2041 catgagcaga ttctctacaa cagcgagaaa tatatggagc agctctatac ggttctcaag
     2101 cccgagctta ccgaagtccg ctatcagagc gaatggtttg gccccatggg aatgaccgac
     2161 gtcctcaagc tgctgggcaa gttcaccctg gcgcagttta tggcccacga caccttccgc
     2221 aaccgctacg aaagcggcct ctcgctggga atgcatgagc tgatgtaccc cgttctgcag
     2281 ggatatgact cggtagccat caacagcgac gtggagctgg gagctaccga acagaagttt
     2341 aacatcctct gcggcagaga tatgcagcgc cacttcggtc aggaacaaca ggtggcggta
     2401 ctgtcgccaa ttctgaccgg caccgacggg gtcaacaaga tgggcaaatc tctgaacaat
     2461 tacatcgccg tcttcgatcc acctgccgag aaatatggca aagtgatgtc gattccggac
     2521 agcttgctga tcaactattt caagtatgcc gcctactcgg ataccgacgc tttggaagcc
     2581 atcaagcgcg agctggaaga cggtgtcaac cccatgctgc ttaaaaagcg cctggccaga
     2641 gagatagtgg gattttacca tggcgaagag gctgccttgc aagctcagga aggctttgag
     2701 aagcagtttt cccagaaaga ggtcccggaa gacctgcagg agtatgtcct cccggctggc
     2761 cccaataaag tgagcaagat cttggtggac tgcggtctat gcgcttccag cggggaggca
     2821 aaacgtctga tccagggcgg cggagtaagc gttgacggca acaaaatcaa cgcctgggac
     2881 gccgagattg agatatctga cgaagctatc ctcaaagccg gaaagcgccg ctttgtgaag
     2941 ctggtaaagt cctgatggga tacgaatttc agcttaagct catcaaggga gcggtgaccg
     3001 ccatcatcat cggagtgatc ttttactgca tcatcctgca tgagatcagt catgccgtgg
     3061 tagcccgctg gctgggagac gacaccgcca gccgcagagg acggctcagc ctcaatccac
     3121 tcaagcacat agactggttt ggcaccgtca tcctgcctct cctgctctat tttagtgccg
     3181 gttttatctg gggctatgcc aagcccgtcc ccatcaatcc atacaacttt cgcaactaca
     3241 agcgggatat gggcttgtcc gctctggcag gcccggttac caacatcctg attgggttat
     3301 ttttcgcggt cctctttcac ctggccacgg gcttttcttt gatccagcag gtctgctatt
     3361 tcatcgtgtt cttcaacttt ctgctggcat ttttcaatct gatcccaatt ccaccgctgg
     3421 atggctccaa ggtgttgggc atggccctca ccgatgaagc ctatatgcgc tggaccgcgc
     3481 aggaacgcac tggcatgatc gcgctgtttg ccatcatcat ggtctccaac cttctgggcc
     3541 tgaatatctt tggcaagctg gtaatgccac cggtgcaggg tttgatggct cttctgggca
     3601 tgggaactta ggcaaccgca aggctctgct attctgataa atacacaaaa aatacataaa
     3661 gaggaaatca tggacatcca aaaactcaag caaatgatcg aacagcatga agtgcaggca
     3721 gttgacctca aatactgtgg tttggacgga cgctggtatc acatcacttt cccagcccgc
     3781 cgcttggaaa aggtgctggt aagcggcatt cccttcgacg gatcttccat cccgggaatg
     3841 agatcggtgg aatccggaga catggtgctg atgccggata cggataccgc tcaccttgac
     3901 cccttttatg agacgcctac cctcagaatg atctgctcca tctgtgacgc tgaaagccgc
     3961 gtcggagtaa agaaagaccc ccgcagcgtg gcccagcgcg ctcacgatta tctgctctcc
     4021 accggaatcg ccgacaacag cacctggatt ccggagctgg aatttcatct ctttgattcc
     4081 gttgaatata acagcgattc tttctccagc ggatacaccg tgacttcctg cgagagcaag
     4141 gactgcctgc cggacgactt tgacgatctg gacggagtgg cacgtcagga tgtgaagggc
     4201 tatcacgccg acaccccttt tgaccgctat tacgagatcc gccaggaaat cgtggaggcc
     4261 atcgaagccc aggacatcaa ggtgagatat catcatcacg aggtagggct ctcctctcag
     4321 caggaaatcg agaccgaact gctggccttc cccaaaatct gtgacgacac catggtgatg
     4381 aaagatatca tccgcagagt ggccctggaa aacggtctct cggcaacttt catgcccaaa
     4441 cccgtttaca atcaggccgg aaacggcatg cacttccaca ttatgctcca caagaagggc
     4501 aaaaacgtat tctataagaa aggcggctat gccgatctct ccaacgaggc catctggttt
     4561 atcggaggag tgctgaaaca cggccgttcc ctggtggctt tcaccaatcc cagcaccaac
     4621 agctacaagc gtctgctgcc aggattcgaa gcccctgtca agctgttcta cggactggcc
     4681 aaccgcagtg ccgccatccg cattccccgt tatgccaaca ccccggaaag caaacgcttt
     4741 gaattccgca ccggtgacgg cacctgcaat ccctatttcg ccatgagcgc tatcctgatg
     4801 gccggactgg atggcataaa aaacaagatc gaccccgcca aatacaacct cgggcctttc
     4861 gatgacaacg tctacgcctg gccggaaaag aaaaaagcca aactgctctc cattccagcc
     4921 gatctgcggg aagccatgct ctgcctggaa aaagaccatg actacctcct tgcgggaaat
     4981 gtgtttaacg aagagctgat cgaatcccac atcgccatga agcgtgtcga gcatgacgcc
     5041 gtctcgaacc gcgttcaccc ccacgagcta atgctgtatt atagtctgta atttaggaac
     5101 agatattgca tctgccttac aatatataca aaaccctgca aacagaggta ctaagaatga
     5161 aaaaaaccgt gctgctactt gtggtcggct tatggatcag cctgtgtctg gccgcgagtc
     5221 ctgagggcac gcctgtcagt tacaaacagg ctttctcgat caccgctcag agcgataacc
     5281 acatggacat ccgattctcg cttccagatt ttgagctgga aacggtgaat gtgggcgatc
     5341 aggtatttga ccggatcatc atgccagacg ccggaacaac tcttgagagt ggacttccgg
     5401 aattgcccac tgtctccatc agcctggcca tccccagaca aggctctacc caagcctcgt
     5461 taatgggcgt tgagcccgag ctgatcaact ccttcctggc ctatccggta cagcagggcc
     5521 aggagctgga aagccccaag agctttgtct ataacgccgg atactactct cagggagctg
     5581 cttatcccca gaatgtggtc cagatcagcg agccttacat cctaagagat ttcaggctgg
     5641 taacaatcca ggttaatcca ttcatataca atcctttgac gcaagcctta acggtaaacc
     5701 agaccatcga tctgaggatc acgttcaccc aggaacccgg gttgaacgag ttggctggcg
     5761 aattcagcaa ctattccctc gcttttgaga aaatctacga agcgtctatc ctcaattttg
     5821 acgactaccg ttatcagatg ttttccagga cgcctccccg atacctgctt atccatggca
     5881 acaatacaga tcccaacttc ctgaacgcga ttaacgactt tgtgctgtgg aaacgccaga
     5941 agggcgcgga tgtggacgtg gcaaccaccg cggccggttc tgccggtact actaccacca
     6001 cgatcaaaaa ctacatccag acggcctaca acaacgtcgc ccttcgccct gactatgtca
     6061 tcctggttgg agatacccag ggatcctata ctatccctac tttctcggtc agcagcggtt
     6121 ccggagatta tccctacacc cacctcgagg gcacagatca ggtcggtgac gtattcatag
     6181 gacgcatctc ggccgaaaac ctctctcagc tgcaaacgct ctttgccaaa atctatctct
     6241 atgagcgcga tctcaatatc aataccgccg attggctgaa caggatgctg ctggtgggtg
     6301 actggtcgcc ttctggcatt tctaccgtat atatcagcaa gtatatcaaa gaaagagctc
     6361 tttatatcaa tcccagttac actttcaccg agctctacgg ttccgatccc tcccccagtt
     6421 ccatgaacac cgccatcaac cagggaatcg gcttctttgc ctatcgcggt tatatcaaca
     6481 tgagcggatg gtcaccttca gaatcatctc tgaacaacgc tttccgtctg cctcacgcca
     6541 ttattcccac ctgcggaacc ggtaactttg gatccagctc tcccggtact accgaagctt
     6601 tcattcggct cggtaccgcc gcccagccca aaggagccgt aactgccatt ggaatggcca
     6661 cttccagcac ccacaccgtg ttcaacaatg tgttgcatgg tggtatctgg ggtggactcc
     6721 tgacttatgg aatgcgcacc atgggtgagg gcttgctgaa cgccaaagcg tatattcatc
     6781 agatgtttgg agtatcttct ccttccaacg tgaccagctt tgcccattgg ttcaatctga
     6841 tgggcgaccc cacaatggaa gtttatttcg gcatacccag cactttcaac gtgactaccc
     6901 agacgaccat ccctctgggg ctcagcctct tggacgtggc agtcagggat aataatatga
     6961 caatcgtggc tgatgccagt gtcaccctgt ctcaggggac cagcattctg agccgcggat
     7021 ataccgacgc ggaaggtaac gtgatcctga tcctgccttc cggcatggga accggaaact
     7081 gtgtgatgac tgtgtccaaa cacaatttca agcccgtgca gattcccatc actgtagaca
     7141 caagtggtac tctggtgcct ggttcaatcg tgatcgacga tgacaccact gctccctccc
     7201 agggaaacac cgacggcttt gccaacggtg gcgaaaccct cgaaatcctc tttggactcc
     7261 agaacacctc tgctgccaca atttccggta tcagtggata cattaccacc accagtccct
     7321 atgcgacttt cagtgattcc ctcgtcactt atgccgccat ccctgccggc cagactggtt
     7381 tcaacgccag tccgatcgtg ctgcatgtgg cacccaacac tcctcacaac agcatgctga
     7441 ggctgcacat catcctcacg gactcgatgg gcaacaccta tgatatttcc gagtttatgc
     7501 ctgtgaccaa cgccaatatc cgttatcaca gctatgccct catcaacgcg gggaccaata
     7561 acgctctcga cccgggcgag acggcaggct tcacggtaac cctcagcaac attggcgcga
     7621 ttggcgtgac gggaatcaca gcccgcctct tcagtgacaa tgatctggtc tcggtcacca
     7681 atcacaccat agaatacggt gatatgctcc aaaacgtcca ggtaacaccc tcggtgcaga
     7741 cttttgagct cctggccagg actcaggtcc tgcccggaat gctgatcccg atgcggatga
     7801 agctttacaa cgcggcaggc tttgagcagt ggctcgcttt taccttcaca gtgggagtgg
     7861 tgactgtcaa cgatccgctg ggtccggatt cctatggcta tgtgatctat gacgatacag
     7921 acaccgctta tccagaatgc cccgtctatg actgggtggg aatagctcct gccgaaggcg
     7981 gagtgggaac gtccctcaat atctcagacg cctataccag cggcgatgag ggagaccagg
     8041 tgggcaacaa tcccctggcc gtagtcaatc ttcccttccc cttccagttc tacgggatca
     8101 gctacagcca gatcaccgtg gcctccaatg gcttcctggt ctttggcacc actgagagcg
     8161 ccgaattccg caactaccgc ttacccggtc cgatgggccc caatcccatg atagctccct
     8221 tctgggatga tttggccacc cacaccggca gcgggattta cacctggttt gaccgtaaca
     8281 accacgcctt cgtgatcgaa tggtacaact tgcgcaacgg tgtaaatggc tcctctccag
     8341 agaccttcca ggtgatcctc tatgatccgg ccgtgcatgc tggctcgctg ggtgacggac
     8401 ctatcaagat ccagtatcac accttcaaca atgtggacgc tacttccagc accagtcatc
     8461 atggctgcta ttccactatc ggcatcgaag accattctgg aatagtgggc ctggaataca
     8521 gctattcaaa cacctatccc accgctgctt cacctctggg caacggcagg gccatttata
     8581 tcaccaacat cccgatctat tacgaagctt cgcatctggt gctgggggag acctacatca
     8641 ccgatccaaa cggaaacggc gtctgcgaac caggcgagct cattgaactg ggcgtgcagc
     8701 tcagcaatat cggcaacgcg gtagccaaca accccaccgc tactctgagc accgacagcg
     8761 aattcatcac catgatcaac gcggaaacca cctatttccc gatcgcaggt gagggctttg
     8821 gcgtcaaccg cactcccttc cgctttaccg tttcacctga ctgccccaac ggcacggtgg
     8881 tcaatttcag catcctgatc gtttccggag aagatagctg gtcgcggcag ttcagcctgc
     8941 gggtggatgc ctctcagctg gaatatctgg gctttatgat caatgacgcg gacaccaatt
     9001 acaacggcgt gatagaacct caggaaagcg tgaaactggt agtgaacgtc agaaatcacg
     9061 cctctgtggc cgcctctgac ctcatggcca cactcagcaa tgccgatccg gcaatcgtga
     9121 tagatgaacc aatcatcacc aaacccgcta tcgacgccaa cgatatcatg cagtttgtgt
     9181 ttgagatctt caacggcggc accagcaccc agagtgaata caaaggcttc cagttcaact
     9241 tgacggcagg caatgggctt ccgctcagcg ccaccatgat ggtccccttc aacatgaaca
     9301 atatcttcag cgatttcgag actgaaaatg gcaacttcga ttccgagacc ggctgggcct
     9361 ggggagcacc ttcgcaggta acaccataca gcgggaccaa gctctgggcc tcaaacctca
     9421 gcggcaatta tcccgatctg gttacctaca atctccatac acccatctac gctcttcaga
     9481 ccgggtccgt gctcagtttc atgcatcgtt acggcatgga aaacaactat gacggcggta
     9541 acatcagcat ctccaccgat gccggaaaca cctggaccct tctgactccc accggtggtt
     9601 atagccacaa ctcgctggca ggattggcag gacagcctgg cttctcaggg atgctggcta
     9661 actggcagcc cgtcaccatc aatctgaacc agtattcaaa ccagatggcc atgttcagat
     9721 tccgcatggg cagtgacgga ggtacgggtg gcattggctg gtttatcgat aacttcgagc
     9781 tctccaacgt gaaccagaag accggctacc tctacggcgt cgtgattcca acttccacca
     9841 ccagccccac cgaagcgatg gtcaaagccg gaaacttcta taccaccaat ccggaccagg
     9901 aaggcaactt caagctcttc ctgcccaatg gaactcactc tgtaaccgcc acgctggatt
     9961 atcaccagtc ctcatccatc cacaatattg tgatcggccc caacgacccc acgcattata
    10021 cggaattcac tctgatcaac ctgcccaagc cggtgggaat cgattttgac gtggataacg
    10081 acagcggctt gatgagcctc acctggaatg agccctatga cccagtcctg cccgtgatgg
    10141 cttataaagt gtacaaacgc ttcaataccg gtccctttga gtttgtccag gaaaccacct
    10201 ccaccaacta tagcgaaacc cttgagctgg aaggtgatta caaatattac gtcacagtgc
    10261 gttatctcaa caccgacggg acccccagcg acctcgtcgc gttctcctat ccctttgtgg
    10321 acagtgacga taacaacaac ccagggctcg tgaccaaact caatcacaac taccccaatc
    10381 ccttcaaccc ctctaccacc atcgcttttg acctggccaa agctggcaat gtgaagctca
    10441 gcgtttacaa cgtcaaaggc cagctggtgc gcagactgac gcatgccaat ctgcctggtg
    10501 gcagtcatcg cgtcacctgg aacggagtgg atgaatccaa ccgtcctgtg gcctcaggca
    10561 tttattacta ccgtctcgag accagggatt ataccaagac caacaaaatg ctgctgatga
    10621 aataatccat cagccaatat actgaaacgc cggtaagtga ttaccggcgt tttgtttgca
    10681 tgagctaata acagtgtatg gaaggtggca ggaccgccct gaatcgctga aatcagtctc
    10741 cgtcagagca attcggaccc acctcctgca tagtcggggt tgggttcgaa gatatcctgg
    10801 cgtgagggtt agaacgcgat aggggagagg aagttaccag ttgcggatag agcagtttcc
    10861 agctcccaaa agctgtcccg cagatctggc tatcgtctcc ctttacttgc gcttgcgatg
    10921 cggtgcgctc ggtggggaaa aacggcggag gccgcacggc agctctacgg caacaaagag
    10981 gcagggatga aggcagtagg gatcagctaa aataatccct gatcctgtcc atctcgtcgg
    11041 gaatggcaat gtggcgcgga cacttggaaa ggcattcacc gcaatgagtg cacttgtcgg
    11101 ccctcatttc aggagagata aaggcttcat actcttgctt atgccgctct ttgttgccaa
    11161 acatcatagc ctcgttgtat tggccaaaga cgaagggaat acccacacca tgcggacagg
    11221 gcatgcaata gcggcattcc gtgcagggga tggcaatcct ttttatatat gctctccggg
    11281 ctttgatata gaggtttctt tccttgtctg tcagcgtctt attactcaga tgtttttcaa
    11341 gcaggatatt ttgttttagc tgctccagag aagacatccc gctcaggcaa acgctgcagc
    11401 ccggaaggtc ccacacccat gcaagagctc tttccagagg tttttgatag aattcgcttc
    11461 cctgccagag cttttccact tcttccggaa tgggggccac cagttttccg cctctgagag
    11521 gttccatgga gatcacgccc atgccttttg aaacggccag tctgtagccg ttcatgcccg
    11581 cctggtaatg ggtatccaga tagtttagca tgatctggca aaactcccag tcaaagccat
    11641 ccacgatgcg tttgaagaca gggtaggagt cgtgaaagga aaacccgata tggcgaatca
    11701 atccatcttt tttggcctga acgagagaat cgaggacctt cagcttcttc atggctgccc
    11761 aactgcgttg attgagcgcg tgcagaagat aatagtcgat gtaaccggtc ttgagctttt
    11821 ccagctgcag tttcagatat ttgaggggat cgcccgcttc cttgaccaac cagcagggca
    11881 gtttcgtggc gacaaatact ttctcccttg ctatgctggc cagaaactct cccagcagcg
    11941 gttcgctctc gctgttgtga tatggccagg ccgtgtcgaa gtagttgacg ccgtgatcat
    12001 aggcgtattg tagcatctcg atggcgcggg gttgatctat tttgccctga tcgtccatgg
    12061 ggaaacgcat gcaaccaaaa ccaagggcag atagtttatc ctgagagccg ggcatcgttt
    12121 tgtaattcat tttcacctct tgagacaatg atgcagaatg gcaggttctt tgtcaacttc
    12181 aaattcagga aggacagaga tgtgatattg atttctaacc atggttatga gggatcaaag
    12241 ctcaacagtc cgtgtaatct tggggcgata actccacagc caggagcttc gagcagtcat
    12301 ctttgacgga tgtactgaat gcttgcgaag aagtgaggga aaaaagaggc cacccgaaag
    12361 cagcctctgt caattcattc gccgatgatt ttgatcagca cccgtttgtg tcgcatcccg
    12421 tcatattcac catagaagac gcgttcccag gtgcccaggt ccagctttcc gtccgtaatc
    12481 gccaccacca cttcgcggcc catgatttgt ctcttgaggt gggcatcggc attgtcttca
    12541 aagccgttgt ggcgatattg gccgtgaggt ttttcaggcg ccagcctctc cagccagact
    12601 tcaaaatcgg catgtagacc gccctcatcg tcgttgatga agattgagga ggtgatatgc
    12661 atggaattca ccaggcacaa tccttctttg acgcggcttt cggtcacggc gttctggact
    12721 tgggcagtga tgttgatata ctccctgcgc ctggccgttt tgaagctgag ttctttgcga
    12781 taggagatca tcaaacgata ggggctttct cgagatactt ggaaagatcg gtgggttggt
    12841 caaacaccaa atccgctcca gcttcttcca gttcctttct gctgcagaat ccccaggccg
    12901 cgccgatcga gatcattcca gcgtttttgg cggtctgaac gtcgacgggg cagtctccca
    12961 gaagggcgat attctcaggc ttgaccttta gcctgtccgc aagctccaga gctctggtgg
    13021 gatctggctt caggggcatg tcaggttgtt cgccgctgta gatcccgaag ggatttttgc
    13081 cgtggttgat catcacgcct ctgaagtagt gacggatcat ttttttggtg aaatagtgga
    13141 ccttgttgga aagaatggcc actttgattt tacgggccac gcagagctgc actaaataga
    13201 gaatcccggg atagggcttg gtgtgtaaaa accagctttc atcgtatttg taccagaatt
    13261 tctccgccag agcatagact tctttctcgc cgcgtttgtc ttccggtgag acactgcttg
    13321 tcaattccgt gagtccgttg ccaacaaagc ctctatagtc ctcaacaggg tgctccggca
    13381 aaccgctttc cgccaatgcc tggttcatgc tctcggcgat gtctctcagg ctgtccaata
    13441 gagtgccgtc cagatcgaag atgatggcct tgatcttgaa tttactgctc atgttatcct
    13501 tccttattga gggtgttaac tttaatggga tcactgtgag agccggagta aagcgacagg
    13561 tgaggatagg gaatctcaat gccagccgcg tcaaaccgct ctttgatgct gattatcaat
    13621 tcattttgca gcgcgaaata gtcgctggtg ttaccccaaa cgccatacag gatatcgatc
    13681 ccggaagcgc caaagcgctg aatgcttgtg aggggttcca tgtccttgag ggcgatttgg
    13741 gaattctggg cgatgtccat gagaatttcc aggacctgtt tgagatcatc gttataggat
    13801 acagatatat ccaaattgac cctgcgaacg gggaaacggg tgatattgat cacctccgtc
    13861 ttgatcatcg tctcgtttgg gactcttaca aaacggttgt ccggagtgtt gatcttgata
    13921 gataacaggt ctatggaaga aatggtgccg attgttgagc ccacctggac tacgtctcca
    13981 atcacaaaag gcttttccga gatcaggaag ataccgctga tgatattgga tacactggtc
    14041 tgcgaggcaa aaccgatggc tacaccgaaa actccagccg cgccaagcaa agctgaaagt
    14101 ttgaagccaa attcgttcag taccgataca gtgagaatca gaatactgac gtaatagacg
    14161 gtcctgatga tgagctgctc gctctgggcc gacaatttat ctctcaccag ccgctggaca
    14221 agctttctaa tcagcttgat cagtggtatc ccgatcgcga gaatcagtat cactctgatc
    14281 cccaaagata cacgctcggg tgttataaac tgcctcacca gggcagctgg atcgaaatca
    14341 ctgaacatta ttacctcatt aatgacgcat acgacttaca cagaccaaaa aaaaagaact
    14401 ccaaaatttg tccatattta ttttagacta attctacatt tcctccaatc ggatttcttg
    14461 acataaatgc gagtgagtga attatgacgc caaaatgtct caggagatta taaatgaaga
    14521 tcattcagct tgtcggagcc cgtcctcaat tcgtgaaact ggcacccatt tcacagctta
    14581 tcaggcaatc ccacaaagag ttaatcatcc acagcgggca gcattatgat tatctgatga
    14641 atgaagtctt cttcaagcag atgggtatcc cggagccgga ctattatctg aatatcggct
    14701 caggatctca tggcgttcag acagcccgca tcctggaagc tctggagccc gtcctgatca
    14761 cggaaaaacc cgactggcta ctagtctatg gtgatacaaa caccactctg gcaggagctt
    14821 tggcatcatc caaactgggg attagaaccg ctcatgtgga agcctgcctg cgcagtttta
    14881 acaaggccat gccagaagaa atcaaccgga tcgtggcaga tcatctatct gatctgttgc
    14941 tggttcccac ccctacagga atgacaaacg ctaaaagcga aggacttctt gacaaagcaa
    15001 gattggtggg cgacgtgatg acggacagtc tgcgcatggg cctcgaagcg gctcaagcaa
    15061 gtggagtttc aagctctcag gccagcccgt attatctgct gacactgcac cgtccttaca
    15121 acgtcgatga tccccaacag ctggtccata tcctcaaatc tctgaataca ttgcaacaaa
    15181 cgattctctt tcccgttcat ccccgcactt ccagacttct ggacgcgcaa acccgcgccc
    15241 aatttgaatt tatccgtttt ctggagccgc tcgggtacct tgaattcctg gtccatatga
    15301 gcggagcgga gatggtgctc acagactccg gaggaatcca gaaagaagcc tatatcctca
    15361 agaaaccctg cgtgactctg agaacggaaa ccgaatggct cgagacggtg gaaagcgggt
    15421 ggaatctgct actgccacct caggacgaga cgttcccctc tgctatacgg actttttgcc
    15481 cccctcagga gcacccctct ctgtttggag aaaatgtcag ccgcgagatc ctcaatctct
    15541 tagtttccag ctgattacgc agattggagt gcagatctcg cagatttgaa ctattaaaga
    15601 ttcacacaga tactacggat tgcacagatt tattaaagat ggtttatagg gttgagaggg
    15661 tttagaatat tttgttacgt agcatcgaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnna actattatag ggccgggtct tcgtactcgg cctgtttttt atagtgccag
    15841 gccagcagaa tcctgtccag gagggcaacg ataccaaaca aagccatgct gagggccaca
    15901 atgatcagga tcacggcaaa gacgtgggcg gtctggaaag atttggtggc ccgcgtcata
    15961 tagattccca gtccggcatt gccacccagc cattcaccga tcacggctcc catcacgctg
    16021 taggtcgctg cgagtttgag gccggtgaac aatgaaggca gggaggcggg tatcctcaac
    16081 aaccgataga tggtccaggt gccggcaccc atcgatctga gcagcaagac ctgttccaga
    16141 ggcacctgtc tgagtccgtc atagaaaccc agggcgatgg gaaaaaagct gaccagcacc
    16201 acggtgaaga ccttggctgt gatcccaaaa ccaaaccaga ggatcaaaag cggtgctacg
    16261 gcaataattg gaaccgtctg tgatacaatc aaatagggat aaactaacct ctttataaga
    16321 gtcgaagcgt gcatcgccaa agcggtgaat attcccaaca gaaaggccag caggaaaccg
    16381 ctcaaactgg ctgtgagagt ggggattaga tgcctgagca taagttgggg ataatctcta
    16441 aagacccgca gaacggatat cggggaggga acgatccaaa atcccaccag atgccgagag
    16501 ctggtccatt gccacagccc caggaccgcg atcaacaaaa gcaatggggg ccaagtctgc
    16561 ttgatgacct tggggctcac cactctccca ctttacgtat ttctgtgcgg agtgaaactc
    16621 caaatcgctg ttggactacg gtctgggcgt gtttgatcag cgcatcgaca tcagcggcgc
    16681 tggcgttgtc cacattgacg ataaatccgc agtgtttatc tgatatcctg gcaccgccga
    16741 tctgaaatcc tctcagcccg cagtcgtcga tcaatttacc ggtgaaatgt ccttccggac
    16801 gtttgaacac cgaaccggca ctgggaagct ccatgggctg tttttcccag cgttgggaat
    16861 cgagctccag cattctggcg ttgatctcct ccggagaatc atacctgagc ttgaatacgc
    16921 tgctgagatg aacgaggcct ctggtctgca ggctgctggt acggtagtca aactggtggt
    16981 ccgcgtgctt gagatggagg ataggatggg gggctgtcag tccctgtgga gtgggttcaa
    17041 gcgctttgct gcaatacagg gcgtctttga tctcgccgcc atacgctccg gcgttcataa
    17101 acaccgctcc tcccaccgat ccgggaatgc cggaagcaaa ttccaaaccg gccagtcctc
    17161 tgtcccggca aaactcgcac agctctctca ggctcactcc acaaaaggcc gaaacatagt
    17221 tttcgtctag gctgagtttg gtgtattttt gggtggagat cacgataccc cggatgccct
    17281 tgtcgctcac cagcagattt gagcctttgc ccaggatcat ccacggtaaa gagttttcct
    17341 gcgcgaaacg caggacctgt atgatctgag cttgtgatga gggaagacaa aacaggtccg
    17401 cggggccccc aatgttaaaa ctggtgtgag ccttcatggg ctcatcaaaa agcaaggcac
    17461 cggaatccag aagttctggt aagtgttgtt tgataaggtc tttcagggtg atagcctcct
    17521 gtctattatc atgagcggga tgtcccaatt tgtctcaggc aggaatatcc tgcttcctga
    17581 acatgatcag gcgtcgtgct ccacatgagg tttccggttg aagaaactgg ttacttcgtt
    17641 gatgagctcg agttcagagc gcagttccag gcagtgagtg ggcagcactt ctttaaaata
    17701 gctgccataa cgtttggtgc tcactctgga atccataatc agcacgatgc cgcgatcggt
    17761 cttgctgcga atcaggcgtc caaatccctg cctcagtctc aacagcgcgt ttggcagcat
    17821 gtaatgcatg aaggaatctt tgccgtctct ctccagcttg tcgatatagg cttccacgat
    17881 cggttcggat ggcaccagaa aaggcagctt gaaaaggatc agcatcgaga gcgattctcc
    17941 ctgcacgtcc actccttccc agaaagaact ggtccccagc agcacggcgt ttttgtgatt
    18001 cttgaactcc tgcaggatgg agctgcggct gccaatttta ccctgggcaa agaatggcct
    18061 gccgcgatga taaagagtgt cgcctatgtg atcatagacg ctgttgaggt ctctgtatga
    18121 agtgaacagc atcatggtgc ccacctctgt ggatgccagg atctgctcca cgcatcccag
    18181 cgcctggttg agaaaaaact tgtccttatg ctccggtaaa aaactgctca ccatcagttt
    18241 ggactggctg tcgtaatcga aaggggaaga gacgatctcg gtaactatct tgtcgctgtc
    18301 gacaagatga aggccgcttt gattgaagaa atacttgaag gaaccccgca aagccagggt
    18361 cgcggaggtg aacacaatgc aggagatctt gctgtagagc agttcgtaga ggattctatc
    18421 cacttccact ggcgcgtagc aaaaactgga accgggagtg ttgcggtctg gtctgggatc
    18481 gttttgcagc caaagagcgt agttttccag atcgggagtc tggaggctca gcagctttcc
    18541 ttccgtctct gaattacgag agtaggtgga ctggatagtt tccaccgttt cttcccaccc
    18601 gtgaatcagc tcggaattga atgatgagta gacgtttccg agcgctttga gatctttcat
    18661 cagttctttc caggcgttga tcaggttgcc cagatgacgg taaagcacat caaatcctac
    18721 cagttcctta atccgcagtt ttccaaaaga ttctgccaga ttcacacggg tggcggcttc
    18781 ctgaaagagc ctggtattca gcgttcgcaa atcttccacg gtattaccca gtttatcaat
    18841 caggagggta gcctggttct tcgctgcgtc cgtgatcgcg cttttcgaca cgttgagctc
    18901 tatctgatgc aacagaccgg agcgtttttt gccactgcct gagagctgaa agagggaatt
    18961 ggcgagctcg gcataggata gttcaaaacc cagcagcccg cttgccgaag ccgcgaggtt
    19021 gtgggcctcg tccaccacca ggtgggaata ctcccccaga gtgctgaatt cgttgcgtaa
    19081 atctgccagc aggagagaat gattggccac cacgatggaa gctgtttcga tgtgcttgcg
    19141 cagtttcatc acaaagcact cattcacctt ggggcatttt ttgcccatgc aactgaagcg
    19201 gtcggaattg atcttgcgcc acagcagact gtaacggccc cggtcaaagg atgagttttc
    19261 agatacatcc ccgctcaggg tttgcagctt ccagatatag agatacatca gcccgcgggc
    19321 ttcccaggaa ctgaggcccc tggtctgttc catcatgatc tcgtcccaac ggcgctgaca
    19381 gacataattc tcgcgtccct tgaccagaac agccttaaat gacaagggaa taaggtcctt
    19441 gaggcgggga agatctttga aaaagagctg ttcctggagg tttttggtgt tggtggagac
    19501 cactaccttg gatttctctt tattggcgaa ttccatagcc gggaccagat aggcaaagga
    19561 ttttcctact ccggtgccgg cttccacggc cagatgcttt ttcccggcaa aagccttttt
    19621 gacagcctct cccatctgca attgcccttc ccggaactca aaattctcaa atctgtgctc
    19681 cagaattcca ttggggccaa agacttgttc catgccgggg atcgtttgtt tttcaacggt
    19741 gtggtcgatt acattcaccc agggattttc aggcaggggc agcttcgcgg cccccaggtg
    19801 acgacgttgc cattccacca gtctgcggag gaattcatgg gatgactcat cagcttgcga
    19861 ttgacggctg aggtcaagaa gcctggcatt agtcatcatg gaatagtgtt cagtaataaa
    19921 cctggccagg ctgatcaata ctttgcctgt cgcctcggca tcggcttccg ccctgtgcgc
    19981 gttcacgagc tctatatcga aatgccggca catggtgccc agagtgtgat cgttggtgta
    20041 gggcagataa atcctgccca gttcagccgt gtcccatttc tcattggtca ggggcaggtg
    20101 gccggaaagc gctaaattat gatcaataaa gccagtatcg aatctcacgt tgtgacccag
    20161 gatcacactt tcccccagaa aaaccaccag cttttgtaaa accgcagcga tctgaggagc
    20221 cgcctttaaa tcgatgggat caatgtgggt gagatgctgg atatattttg ggataccgtg
    20281 tttgggcttc acgaagctct gaaagcggtc aacaataaca gaccccgtga atctcaccgc
    20341 ggcaatctca attatctcac agttttggaa atccaaaccc gtggtttcga tatccaaagc
    20401 gacgaaatcc agtgtacgga ggaattcctc tcccggactg gtcttgatat ctggagggat
    20461 ggcagtcatt attcttcgcg gttgaggttg taacggctca gctttttgat caggccttgc
    20521 ctggaaagtc ccagttcttt ggccgcctgg gtttgattcc agccgcagcc ttccatgcaa
    20581 tcatatatca tcttacgttc cagttcctcg attgcgtctt tgaggttttt cttgcctgaa
    20641 agcatgctga tagtgcggat gttttccata aaacggcgca cttcgtcaga gaagtcgctg
    20701 ggtttgatga aagatcccgg ttcagccagg gttacagccc tctcgatctc attctcaagc
    20761 tgtcttacgt ttccaggcca gtcatactgc tccaggatct tcatggcctc atctgtgatc
    20821 ccgggaactt ctttgccgat tcttttggtg tatttgtcca ggaagtgaat agccagaagt
    20881 ggaatgtctc cctggcgtcg cttcaaaggt ggcacctcga ttctgatcac attcagccgg
    20941 taatagaggt ccagccgaaa atcaccttct ttcactttgt caataagcga gacgttggtg
    21001 gcacagacca ctctcacatt cactttttcg gttttggtgg cccccacccg cttgatttct
    21061 ccttcctgca aaaagcggag cagcttcgcc tgggtggatt gactgatatc ggctatctca
    21121 tccaggaaaa acgtccctcc atcggcgatc tcgaacagac ctttcttgtc ataggccgcg
    21181 cctgtgaagg agcccttgac gtgaccaaac agctcgcttt ccagcaatgt ctcaggcaaa
    21241 gcgccgcagt attgcgccac gaaagcttta cccctgcggt tgctgctgta atgaagtgct
    21301 ctggcgatca gttctttacc agtaccgctg gggccctcta gcagcactga agtgggtgta
    21361 tccttcacct tttcaatcac ttcaaagatc cgctccatct cagggctttt accgataata
    21421 ttggcataga ggttgccggc attcagctgt tcgcggatga tggcatgagt cttttccaga
    21481 ttggcgctgc ggacgttttc gatgatcacg atcacctgat ttgatagcgc gtttaagagg
    21541 tttacaatcc tttcagagaa gtaatgcgag cccccctggc aaaagagcag caccaccccc
    21601 aaaacgcttt tccggatgga gagggggatg catataatat tgttgtaaga aggggcaaaa
    21661 tgaggctgct tgaggtccgc ggctttattg tcgcggtaga cgtccgtgca aatacccagg
    21721 aaggtctcat ggagcttgtc atccggagag aaattgcggt agattttata atcccagtgg
    21781 tccggcatct gttttccagt atgcaagcac aagatgcctc cgtccgcttc ggaaatctcc
    21841 accagactgc tgagcgactg ctcgatcaac aggtccagat cactgatagt gttcagtttc
    21901 tgggtgatct cataaaactg gttcaggagg ttctcttcaa acttgacgtc ctgcatctgg
    21961 gcggaatact cccgcgcaat cttctggctg aggatgtcat tctgttccaa cagcttgatg
    22021 gagccgtaac gctggatgat cttcttgtta ttcttgagga tctgcagcgc ttgttcccaa
    22081 tcctggagat caaagagcac gctggccagc tcgtaattgg ccagagaaag ctcataatcg
    22141 ttgtcggttt ccacgaagtg gcggatagct tcatcaaggt gctcgcgggc ttgttccaga
    22201 tttttgcgtt ccagcaatgc tctcagataa tgagcccttc ccacctcaaa agagcgattc
    22261 tgatcggtgg ccatcttgag cgcctgggtg atatagtaat cgccgctctc gtagttgtgg
    22321 gaaaggatga agtaaaaggc ctgcttctga tagccctcga taatcttgtc gcgcgcgttg
    22381 agagacttga aaaaatccag gctttctacc agataactgt aagcctcctt gaacttttgc
    22441 agccgggtga gtacgctgcc caggttccca aatagttccg ctttgatgta ttcatcactc
    22501 acagaaggca atagctccag tcccttgaag tataggtcat atgcgagatt cagttctcct
    22561 tgtttctcat atacttcacc cagattattg aatgacctca gcagcccctc gctgaagttg
    22621 ttatcctgag atttgagaat ggcctgttca taaagttcga tggagcggtt ccattccccc
    22681 atcttgaaat agagcgcgcc gagattgttg agcgaagcca cggtattgcg gtagaaacca
    22741 aattccaccg cagtctccag tgagcgcttg agggcgtctt ccgctttgga ataatctccg
    22801 tggtccatca gcgtaacgcc gatgttgttg tagatatttg tgatgttgta gggctcgttc
    22861 agcattttct gcagcttgag cgcttcttct agatagaata gggaacggct ggcatcaccc
    22921 ttgtcatcca tcagaccgcc caggttgttg tagcacacgg atatgccgtt gagattgaag
    22981 tgctctttct ccagagtgag gctgcgcagg aaaaactgct cgctgcgctc ccaatcgccc
    23041 tggaacatat agaggacgcc cagattgttg tagatggcag ccaggcccac gtgatcgctg
    23101 gccagattgt acatcgtttc ggcgtccttg aaagcttcct cgctctggtc ccattcattt
    23161 atgtaataat ggatcttgcc cttggtcttc ttggcttcgg cctgcacctg atagcgcttg
    23221 atcttatctt ccactttgct gagagaatgc aacacgttct ccaagacttc gagggcctta
    23281 tcaaattcct ctccctcagc cagaatatct gctttcagca gcaggatttt tatcttccaa
    23341 tactcgtcga tgctggcggg agtatacttt ttgatgattt cgagagcgaa actggatgag
    23401 ttttgggcga agagattgga ggccagatgc cagacgatct cctctgcagg caaagagttt
    23461 tcagtgcaga cctgtagggc ggcccggaag gaatccactg ccgcgtcttt gtcgttcata
    23521 ccggcagtgg ctattccaat gtgtttttgc gcttccagca attcccgtgg ttccgttacg
    23581 tgcctcagga tgtagcgcca cagctccgcg gccccgcttt catcagaaat cgattccaga
    23641 taggcggtga gccgcgaaaa cagctccgga tcatagatat tgctcatcat catgagcctc
    23701 accagcactt gttcgcagcc gcttctgcct ttcaacactt tgatgacgcg ttgtttgagc
    23761 tctggactga actgctctgg atgctcgtca atccacctca tcagcgagct ctttttctgg
    23821 accgcgtagc tggattcaat gcggcccagc agatcttcac ggatcaatcc ctccgtatca
    23881 tttgtgaggt ttttactgat gcccaggcct ttagcgacgg cagcgtcaac atcgttgttg
    23941 aacacgatca tgctgatcag gaggtcccgc tggctgacgg ccagtctatc cagacttcca
    24001 aaaaagagct gggtggggtc aaaatgcctc tccaggaaag gtgagaggtc aaaacctgta
    24061 tctttgacca gcatttcgtt gagaattcgc tccaccacca tcagattgcc gtctgagatg
    24121 cgttgcagta tttcgctgcc gctcacgtaa cccaactgct cgttcgggaa agccacctgc
    24181 aggatttgct gcagatcgtc ggtcgtgaga gatgggatgt attcaaacac ggcaaagggg
    24241 aaaaggcggt tttgcgaaat ggcgatgatc tggatgcctg cttcctcggc atagtggacc
    24301 agatactgca gaaactcgcg gctaaagctg tccagcacat cgcagtcgtc cacgatcagc
    24361 accatcggtt ttagcatctc gagttcgttg agcctggaag tgagaaagta aaagaagtca
    24421 tgcttgcggc cggtgcggta tctggactga tgttcctgga tcagctcttt tagctgttga
    24481 gtctccaggt cggtgaccag ctccacaatc tcctcaaact ggttgaagct cattgggtta
    24541 ggagaaaaca cccttacccg ttcgtatttt accttcagct gctccaccaa cggactgatc
    24601 agatatgatt tcccggatcc cgcccttccg gaaagctcga tcaaatggct ggcagagctg
    24661 ttttttaata gttccagcac tctggaactg aggaagcgtt cagatacgaa ctggtgcatg
    24721 atcctgtaaa gcctttctgg catcttcacc tcacataaac aaaaatctgt cagaccgatt
    24781 tctcagtttg tttacaggga ctgtaaattg aatttacact atgtgtcaag ccttttttca
    24841 cggatgttga caa
//
LOCUS       DAOSFN010000016        23341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1739_length_23341_cov_8.751267, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000016 DAOSFN010000000
VERSION     DAOSFN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 23341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23341
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1739_length_23341_cov_8.751267"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1422)
                     /locus_tag="PLO35_02195"
     CDS             complement(10..1422)
                     /locus_tag="PLO35_02195"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOH78502.1"
                     /translation="MKKKEAKTTGLLYERKNFFKDASEADRAAAFQYAEAYKDFLNQA
                     KTVRETVAYVESQLKKHKFTPLGAKNAKNRVYTIFRGKTMAFAVLGTEPLSSGFNMVA
                     AHIDAPRVDLKQNPLYEDNSSATACMRTHYYGGIKKYQWVSTPLALHGAIVKKDGTVL
                     QISLGEKDNEPVFIIPDLLPHLARKEQYSKNITDAIDASKMNLVFGGMQEPGNDDKEA
                     IKAHALKLLNERYGISEADFLSAELELVPASKSRDAGIDGSMVVGYGQDDRICAYSGL
                     TALLDSLETKPKRAMIVYLSDKEEVGSQGSTGAHSIFVQDFVASLMKASGESDSSSNL
                     RRAFMNAHIISGDVTAAIDPNYPGVHERQNAVLFNHGVGVAKFTGSGGKYGCNDANAE
                     FTARVIRIFNEAGVFWQMGELGKVDEGGGGTIAYILANLGAEVIDCGTGLMGMHSLYE
                     LCSKADLYSTYRSYKAFLQS"
     gene            complement(1454..1936)
                     /gene="greA"
                     /locus_tag="PLO35_02200"
     CDS             complement(1454..1936)
                     /gene="greA"
                     /locus_tag="PLO35_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOH78503.1"
                     /translation="MDLSLFITQEGMDKIQRRIGHLMNNERPETIKAVAIAREFGDLS
                     ENAEYKAARERQRAIDGEIDHLRRRAARLKVVDTSVFPKDLVRFGSICRTEDLDNGEA
                     VVFKVVGADELNFYEGEPFQPVSALSPIGKALLGKKPSEVAVVQAPMGDRHLKVLEIL
                     "
     gene            complement(1941..2747)
                     /locus_tag="PLO35_02205"
     CDS             complement(1941..2747)
                     /locus_tag="PLO35_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78504.1"
                     /translation="MLTIDWKERLAKDTQDYLNNKLPNQDYDFDIIFNAYPERVNGKI
                     PSEVIAYVSGIIISKLGKDHEKYLPFYRYIWDKKGDHGRGAFIVIMARLMPKKPAVYL
                     PLLEHAMQRSNPTELASILERVMLPLLRKAPQTYLPTVYGWDRSPREDIRKANVNLMI
                     KLMKKEPDTIGPIITHYTNQWLQPLGDAQAHHVTLLKALYKIDAARYLEVWAHHGHQR
                     DPQTVEILCAALMDYDPGIEASVETWTHSGNARVKKAATTAMRVLKKKKG"
     gene            complement(2817..6014)
                     /locus_tag="PLO35_02210"
     CDS             complement(2817..6014)
                     /locus_tag="PLO35_02210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH78505.1"
                     /translation="MARLFSKDYHEHSASWYFNHAIIVLEAGDLEYWRSVDEQGLYHL
                     RFFPRPEVRHHFCQRLDLVYDPVSQLIVRHECSECGEEELCRHFLSLLRYAYHHLKTD
                     IFDSPAVETCSRDDLRVSEDWSETLSSARLELEGLYDPGADKVRFYHESFAPIDLPYL
                     VRMAIGDSLESYPARQKEEYLRNLGAISGPRMSFLSWLQLNKSAYSFKSKFYSIYKRD
                     LGRALSLLMNCGDLIRIRETGETLKLHTQPYPLSLRIEPAGKRNFVVLPVIVDELSAW
                     IAGYPTWLVMRDHVYPVWFPFSDQKTELLMGRQLLISPKDLVYYRTVVHHELRRHDIY
                     LDFDPGIELPQIVDSEPRISLELKRGGDLLIMEGWLRYPEGEEVPLSVTRFNSSLVRC
                     DFRKDSQQGNGWFYLPPRIFQQVKDLMGRFPEADRRRVEEYSQISFQGDTAINALRER
                     VFSMGDEDWDLVIAEDLGDFFVQKVPISVQIDARRGDEIDWFSYDVRYSYRDYSFSHE
                     ELKRFFRSNEEFMHTKDGRLIFISNREVFDEMERLISRSEKYKDSIYRARLVELPYYQ
                     RLMMDRPAIRLIQDEWLDKMSADLLRRHLEKTPALPVNLQTILRGYQKAGVAWLKMLQ
                     HYGLNGILADEMGLGKTIQALSVIHGTPEGRVSLLICPKTLLYNWAAEIEKFHTNIPY
                     LVVEGGKSIREDMLREPNVKLLLISYSLVLNDIALLRQINFYWIVLDEAQNIKNVSAQ
                     RTAAIKKLGSEHRLALSGTPVENNLTELWSIYDFLMPGYLRSLKRFRDDFIGAEDSDL
                     AHQRLHRMVSPFLLRRIKKEVLLELPDKQEQVSWCKLGSLQEKTYLQVIELVQKQLFP
                     AGTSGAVNYIHVLAALTKLRQICNHPHLVNPDINPDPEASAKLEQLLELVGDSLASGH
                     KVLVFSQFVQMLTIIRACFEARGIMYSYLDGQTKDRMAVVNEFEGSSEKRVFLISLKA
                     GGTGLNLTSADTVILYDPWWNPMVENQAIDRVHRIGQTNKVQVFRLISKGTVEEKILA
                     LQQSKLAMFDAVISEGQQLLGSLSSEDIRGLFSYQ"
     assembly_gap    6405..6504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6505..7026)
                     /locus_tag="PLO35_02215"
     CDS             complement(6505..7026)
                     /locus_tag="PLO35_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78506.1"
                     /translation="MENETSKKFSWKKLWIIAGIVVLAILAFLIFGMPRLKAMQAKKL
                     SAEAKTALEALKLNVDNYWKTHDGAGGFDIAKALEDAKIEKKAQERWDFVIAWKPTDI
                     YTGEMMGKLNDLNQANYVYVAPYKMIMAVAKAKNPAGEGAKMWFSGDENQYHGFGLDG
                     MVEPDWHKIFPNP"
     gene            7569..>7940
                     /locus_tag="PLO35_02220"
     CDS             7569..>7940
                     /locus_tag="PLO35_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78507.1"
                     /translation="MLQDLFSAPVRLRAGICAGRTAMFLVFFILAITGTGLWGTIQTV
                     VYNVGNIPSTFWVNPSTTKRADQPGLLSVSIPVGQKIASMDIQYSMSTANGSWMSEQN
                     SFLLCSTNGLTEEEVYSGEGDT"
     assembly_gap    7941..8040
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8041..10159
                     /locus_tag="PLO35_02225"
     CDS             <8041..10159
                     /locus_tag="PLO35_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78508.1"
                     /translation="HSFLTLPADAQILVYDMGNIPSTYNTDVSTSSRADSPGLLSVTI
                     PPNKEIKHVGIQYSMSTDNGSWMSEQRSFLLCSTNGATESEVWEGEGQRTGICDYRRD
                     HLNLARGLSGVVDFELHAFRTWGGSGTDTDYQYVMNGSWQLIIELEEYPVLDYPSDLE
                     AFDVGPGSAVLSWEDNCYPNATEWDIMYEPYSYEFGSSRSLISNVTSNPYLLTGLVSG
                     QQYLWSVRANKGDLGVSDWADDEFFTTLWDTEAITYTLGNIPTTLVHPDSIHVTNRAL
                     EPGIMEVTVPPGKMISGVDLDYAMTSNGSGSVYDQRSFLVCSTNYGNEPVVFSGSGSS
                     NTCYYSREGLHLANGLSGTVVFELHAFRTRCGTGSNTDYNYVNNGSWTLTINYADRPP
                     SDLSASNIGYNSAQLNWTENSFPAAPTWDILYGPIGFDRLRSGTLISNVSSRPYTLSG
                     LYSYANYEWMVRSRSNSDWVSDWSAPHTFTTLLEPGDVILTYNLGDIPTTYEIAPTTS
                     SRALEPGLLSVTVPTGKRIERIGIRYIMSANMGGYICHQRSFLLCGTNGVTEPEVFEG
                     SVPAQGMQYYGRDDVSIANGLSGVVDFELHAFRNWGGTGSNTAYNYVYNGTWALIVHY
                     EDIPVVLLAPVVTFSAPAGVPQLSWTAVPNANSYKLFGSDDPYSSAPWRLVTITPATT
                     LSYTPFLSGTQKFFRVQASSDMP"
     gene            10356..11564
                     /locus_tag="PLO35_02230"
     CDS             10356..11564
                     /locus_tag="PLO35_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH78509.1"
                     /translation="MRRKQVTEIIALVYTPEIILLTGARQVGKTTAMLQVHEQLRSTG
                     LDAHFFTLEDPDYLLLLNQHPDQLWRIVPKPIDGKTVVIIDEIQYLAKPSNFLKYHYD
                     LNRDKLKLIVTGSSAFYLDERFNDSLAGRKRVLLMPTLSFDEYLRFKGDTSLAECFEQ
                     AAITEPDLSNLSIPQQREIFRYWDDYIIYGGYPAVVLASDSREKERLLNEILSSYTKR
                     DFLESGIQVPDKAYQLMRILAHQSGSELNRSKLSVELGVARSSVDTMLRILQKSFHVS
                     LLNPFFGGHPKELRKMPKLYYLDHGLRNCLCKNYLSIEGRMDTGRLFESYVYRHFFER
                     KGPEELRYWRNQQQQEVDFIVSEQAAYEVKWNGDAIQTSKYQSFSKLHPNLPLRYIVR
                     RNPGEDCLLF"
     regulatory      11614..11814
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11877..13118
                     /locus_tag="PLO35_02235"
     CDS             11877..13118
                     /locus_tag="PLO35_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78510.1"
                     /translation="MTRKTLCLIIMITLASWGLQAAIIYAVNSESRTLSRIDTDSQSI
                     DNSFAQLGLTPNLMTLDEDHIWVVCSGDNTIQELDRQTGSLLRTLPVAPSCNPWDVLK
                     IGDYLYVTGLFTDKLYKLSLASGTVVGSLQVGTAPEGLAAYGDLLFVANTGGYQNNYA
                     NSSVSVVDLSDFSVVNTIPVWSNPQYLKALDGRLHVSCTGNWSDLSGKIDVIDIPSQE
                     HLARIDLNGRLMSVWFSSEGIALVGEALSTGFYRYDAHSLSILNSISNPLQPGAYAID
                     GNQELVALMRQNWGSPSLLYIRHPDLSAWQQYNLGLVCTDIKIYEAPTAVSDDAVTAP
                     GLNLYPNPARRSELLKLDNRSNKRSELRLYNLRGQMIHSQILAPGLNELPLAPLTLAP
                     GLYFYRSGLGKSAVNGRLIIR"
     gene            13255..13893
                     /locus_tag="PLO35_02240"
     CDS             13255..13893
                     /locus_tag="PLO35_02240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOH78511.1"
                     /translation="MTLQIRRENPEDYSAVEALTRDAFWDVHVPGCDEHYLAHILREA
                     ECFIPELDLVAIYEGTLVGNIMYSKALLKLDSGGALELLCFGPLSVAPAFQRKGIGKA
                     LVEKSLELAGGMGYGAVIIYGDPDYYRRLGFRPGEDYGIRGQEGMYSPALQVLELVPG
                     ALKGLHGSFFEDQIYQLDPEAAARFEQGFPPREKGFRPSQLRFQEMLAQAHY"
     gene            14473..16938
                     /locus_tag="PLO35_02245"
     CDS             14473..16938
                     /locus_tag="PLO35_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH78512.1"
                     /translation="MRRVLVPLLLLMLSGFSACRSTGRIMTRVLSPRYLQRTDDPIIK
                     AVFADLSGNGRDLLVTAESSHPASVILKAYDHDGRLIFEHDTRQQVRDIKALANPKDG
                     GFWIFYTHSEQEGLLLKAVRFDRDADDQHQSRSFEAIPIFTTSSASTTGNGNLRLEPL
                     NIIDLDQDGKAELVCKLSSNIPGEARGLAVMDFGSGRLEWSLKTESNLTDVLIDDLDN
                     DGSVEIVLGSRVEQASGNDAVPFNRSGGWLAVLGPRGDLLYSERQFQGSGEVRLASVK
                     PAQSPTPLIFCVSNTWGPRRTVNAVSSRFWSGKKLVPTREISTSNSFERAGDLPNLAV
                     TEFDRKPRLILIERGTGIVSYDEELKRTIHTCPDFVNEILDVKDIDLDNKPEIIARRP
                     DRTILVLDTDLHKLAELSNPYPTADKVNVYVVGRGSGLAKGLAVYSEELVTYYGYYPV
                     NWFTLLGRFTTANSVYFAIFMLFLLLLMIWYVIYNWQSIYTIMPALRVAVIYLRNHDR
                     LMYCNPYFLELVRAPVGQKTRSLKALSPTLGKMLIDFRASGNRLQNHELALGEDQMAG
                     IYRVSFYRGHNPIYRYLMIIVPVPTVNAAEKILWADTARRLSHHVRRHITNVILALPK
                     LTDEDIDSSERKEYHQIISAEVEKIRVFTHSFQRFSELRDHDLRQHDVLPLVEHVLDH
                     LHIQEGVKLIRAIPGSPLPAYLDPIRFEEALANLLNNALDSMPSGGYLQITAKTVASQ
                     ESPKSDLRVLIEIEDNGNGIPEKYMEEIWKPFFTTKQSGTGIGLPESRKIIESMGGMI
                     TLHSEERVGTTVSIWLRVGESEG"
     gene            complement(17008..18129)
                     /locus_tag="PLO35_02250"
     CDS             complement(17008..18129)
                     /locus_tag="PLO35_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH78513.1"
                     /translation="MIKRKIFDSVNEWLIRNKIILILGPRQSGKTTLLNMIQDQFQGQ
                     SQYFNCDEQEPRTLLSSQNSAQLRSVIGQNKLILIDEAQRIENAGLNLKIIHDTMPDI
                     RIVATGSSAFELADKLNEPLTGRKIEYNLLPFAFEELVADGSMIHELQQRPNRLLYGS
                     YPEVVLNSHLAVPTLQNLCGSYLFKDVFSIHDIRKPELLDKLVQALALQLASEVSFHE
                     LAQLLDSDSTTIERYITILERAFIVFRLPNYSRNQRNEIKRSRKIYFVDNGIRNAILA
                     DFRPLELRDDIGKLWENYIVSEFHKLKLNRVLYHHSYFWRNRNGAEIDYLELANDQLS
                     AYEIKWNPKKQGSFRAFLNLYPEALTETVTPENYFLHLN"
     gene            18396..19541
                     /locus_tag="PLO35_02255"
     CDS             18396..19541
                     /locus_tag="PLO35_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOH78514.1"
                     /translation="MDYFLTEDQIEMRDLARRIAIEKMKPVSEKYDEEGIFPWDIVEV
                     MKQSDLFAILIPEEYDGISGKVVDLAVVTEELCAVDAGIALAFGATGLGMYPILISGS
                     EEQKHKYLSQIAAGEQLAAFALTEANAGSDAGAIETTARKEGDNYILNGTKQWITNGG
                     EAGIYTVFAMTDKEKGARGCSCFIVEKGTPGFSFGKKENKMGIRSSATRELIFEDCVV
                     PAANLIGREGTGFITAMKVFDKSRPMVGSQAVGIARGAFEAAVRYSKERQQFGKPISS
                     FQAVQFMLADMATQIEAARALVMQTARMVDSGAKNYSKESAMCKYYASDVAMKVTTDA
                     VQILGGYGYMKEYPVEKMMRDAKILQIYEGTNQIQRGIVAANLLKEY"
     gene            19636..19968
                     /locus_tag="PLO35_02260"
     CDS             19636..19968
                     /locus_tag="PLO35_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOH78515.1"
                     /translation="MEWIKHFPHAVTVADPDGKVTFMNDKAIQTFADHGGAALIGSSL
                     YDCHKPSSQEKIKHMLATGESNIYTIEKAGVKKIIVQQPLLDNGRVTGIVEISIILPA
                     EVAHFVRS"
     gene            19955..20500
                     /locus_tag="PLO35_02265"
     CDS             19955..20500
                     /locus_tag="PLO35_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOH78516.1"
                     /translation="MSEVKKLLLHTCCAPCLIAPYQELKARGIEVTAFWYNTNIHPYT
                     EYKARKETLEAFSASEGFPLIMRDEYGLEPFVKAVVSDIADRCRYCYETRLAATAKLA
                     KEGGFDAFSTTLLYSRYQKHELIVETARRYSDLYEVSFFYEDWRPLWHKGIELSKAEG
                     MYRQKYCGCIFSEQERYLGGK"
     gene            20500..21453
                     /locus_tag="PLO35_02270"
     CDS             20500..21453
                     /locus_tag="PLO35_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78517.1"
                     /translation="MSRYKQIDLDNIKVYSVKDRPSKVEPELFAKPANSDMTGFLASL
                     PKLLKAQELNEFIGHSVEAARLKKGFIIGLGGHVIKCGLVPLLIELMEQGIATCFAVN
                     GSVAVHDYELAMFGKTSEDVAAKLADGSFGMVYETTYGINSVITKASETGLGFGEALG
                     KTILEKAPHPEYSLLAMAYKHQIPVTVHVAVGTDILHQTPCADGKAIGDCSLRDFRIF
                     CEEVSKLSGGGIYLNLGSAVILPEVFLKALTVARNIKGRVQDFYTAVFDMNTHYRAME
                     NVVKRPVLNGGKGYYFIGHHEIMIPLYIAAILEQFDLDIPF"
     gene            complement(21471..>23341)
                     /locus_tag="PLO35_02275"
     CDS             complement(21471..>23341)
                     /locus_tag="PLO35_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOH78518.1"
                     /translation="KKLADKLFKEYKGRIPQEDVSARALVNGYYYFNRQTSKQDYPVY
                     LRQKDAPGAKVETLLDLNKMAKGKQFFDVGLSAVSPDNRVLAYTADLSGAEVYRLYLK
                     DLGTGKITDTKLDRIADFVWQLDSRHAIIVTENDIMRSDKCLRLDLDTREQTLLYEEK
                     DPAFNLGLYRWGDRSWVFLSAGSKNSSEAWIMPLDDLTGSFQAIAGRRPELEYSASIL
                     DKELYIHTNLWNPDYSIAHCSLNASAIEDWQQYISAPQGASIWDFSLFDDKVVVETMR
                     GGHDQLAVYERSTGKLLDEIIPEQPSDLGLWNNLEPGAPGFNYYSESWLRPYQIYQHG
                     FDTKADSLIYTKALPKGYDPDKYTSSASYYVAEDGTRIPLTLIHKKELPEGPKPVWLN
                     GYGAYGDVESPWFSSSRQSMLDRGVIFALAHIRGGGEMGKAWHDAGRLLHKKNSFTDF
                     RDAVEYLISSGLTTPGQLLIEGGSAGGLLMGAVTNMIPTGVKAVIADVPFVDVINTML
                     DPDLPLTVGEYAEWGDPNDPKYFSYMLSYSPYDNVGPEVYPQMLISSGWMDYRVGYWE
                     GLKWAQKLRLNNMGDSRIIYLLQKDEGHSGSSKRYASLKSYARSVAWGISQITGLK"
BASE COUNT         5393 a         6185 c         5977 g         5586 t
ORIGIN      
        1 gatatctttc taactttgca gaaaagcttt atagcttctg taggttgaat acaaatcggc
       61 tttgctgcac aactcgtaca gcgagtgcat ccccatcaga ccggtaccgc agtcgatcac
      121 ttcggcgccg agattggcaa ggatgtaggc aatggtcccg ccaccacctt cgtcaacttt
      181 gcccagttct cccatttgcc agaaaacgcc ggcttcgttg aagatcctga tcactctggc
      241 ggtaaactcg gcattggcat cgttgcagcc atactttcca ccgctgccgg tgaatttggc
      301 cacacccact ccatgattaa agaggaccgc gttttggcgc tcatgcaccc caggataatt
      361 gggatcgatc gccgccgtta cgtctcccga gatgatatgg gcgttcatga aggcacggcg
      421 aaggttagaa gagctgtcgc tctcgccgct tgctttcatc aggctggcca cgaaatcctg
      481 cacaaagatg gaatgggccc ctgtgctgcc ctgactgccc acctcttcct tgtctgaaag
      541 atagacgatc atagcgcgtt tgggcttggt ctccaggctg tcgagcagag ctgtgagacc
      601 tgaataggca caaatccggt catcctgtcc atagcccacc accatgctgc catcgatacc
      661 ggcgtcacgg ctcttactgg caggcaccag ttccagttcg gctgagagga aatccgcttc
      721 cgaaattccg taacgctcat tcaacagctt gagggcgtgg gctttgatag cttctttatc
      781 atcattgccc ggttcttgca taccgccaaa caccaggttc attttggagg cgtcgatcgc
      841 gtcggtgata ttcttgctgt attgttcctt cctggccagg tgaggcagca ggtctggaat
      901 tatgaacacg ggctcgttat ccttttcacc caggctgatc tggagcacgg tgccgtcctt
      961 tttcacgata gctccgtgca gagccagggg agtggacacc cattgatatt tcttgatgcc
     1021 gccatagtaa tgggtacgca tgcaggcagt ggcgctgctg ttgtcctcat agagagggtt
     1081 ctgcttgagg tccaccctgg gagcgtcgat atgggcagcc accatgttga acccggaact
     1141 cagcggttcg gtacccagca cggcaaaggc catggtcttg cctctaaaga tcgtatagac
     1201 acggtttttg gcgttttttg cccccagagg ggtgaactta tgcttcttta gctggctctc
     1261 cacataggct accgtctctc tgacggtttt agcctggttg agaaaatcct tatacgcttc
     1321 ggcatattgg aaggctgcgg cccggtctgc ttctgaggcg tccttgaaaa agttcttgcg
     1381 ttcatagagc aacccggtgg ttttagcttc ctttttcttc attaagactc catatattct
     1441 atttcttgat gttttataat atctcaagca ccttcagatg cctgtccccc atgggggcct
     1501 ggaccaccgc aacttcggag ggctttttgc ccagaagggc tttaccgatg ggagaaagag
     1561 ccgaaactgg ctgaaatggc tcgccttcgt aaaagttcag ctcatcagcg ccaaccactt
     1621 tgaagaccac cgcttccccg ttatccaggt cctcagtgcg gcagatactg ccaaagcgga
     1681 ccaggtcctt gggaaagaca ctggtatcca ccactttgag cctcgcggcc cggcggcgca
     1741 gatggtctat ctctccatct attgcccttt ggcgttcccg ggcggctttg tattcggcat
     1801 tctccgaaag atcgccaaat tctctggcaa tggcaaccgc tttgatcgtc tcagggcgct
     1861 cgttgttcat cagatggccg atgcggcgct ggatcttgtc cattccttcc tgggtgatga
     1921 agaggctcag gtccacccgc tcaacctttc ttctttttga ggacccgcat ggcggtagtg
     1981 gcggcttttt tgacccgggc gtttccgcta tgggtccagg tctccacaga ggcttcgatg
     2041 ccggggtcat agtccatgag ggcggcgcag aggatctcca ccgtctgagg atcacgctga
     2101 tggccgtgat gggcccacac ttccagatac ctggccgcgt cgatcttata aagggctttg
     2161 agcagggtga cgtggtgggc ctgagcgtct cccagaggct gcagccactg attggtgtaa
     2221 tgggtaatga tggggccgat ggtatcgggt tctttcttca tcaacttgat cattagattg
     2281 acattagcct tgcggatatc ttccctgggg ctgcggtccc agccataaac ggtcggcaga
     2341 taggtctgag gggctttgcg caacaacggg agcatcactc tctcgaggat ggaagccagt
     2401 tccgtgggat tggaacgttg catggcgtgc tccagcagag gcagataaac ggccggcttt
     2461 ttgggcatca gcctggccat aatcacgatg aaggccccgc gaccgtgatc cccctttttg
     2521 tcccaaatat agcggtaaaa ggggaggtat ttttcatggt ctttgcccag tttcgagata
     2581 atgatcccac tcacataggc aattacttcg gaagggatct tcccgttcac ccgttctgga
     2641 taggcgttga agatgatgtc gaagtcgtaa tcctggttcg gcagcttgtt gttcagatag
     2701 tcctgggtgt cttttgccag ccgttccttc cagtcgatgg tgagcatggt ttctccttcc
     2761 tgtttttttt tctcagaatg ccaaaggcga ctgtggctta aaactgtcaa gagaaattat
     2821 tgatagctaa acagcccccg aatgtcttca ctgctaagcg atcccagcag ttgctggccc
     2881 tcggagatca ccgcgtcgaa catcgccagt ttgctttgct gaagggccag gattttttcc
     2941 tccaccgtac ccttggagat cagcctgaac acctgcacct tgttggtctg gccgatgcgg
     3001 tggaccctgt cgatggcctg gttttccacc atgggattcc accaggggtc atagagaatc
     3061 accgtgtccg cgctggtgag gttcaaacct gtgccgcccg ccttgaggct gatcagaaag
     3121 acccgctttt cgctgctgcc ttcaaactcg ttgaccaccg ccatgcggtc tttggtctgg
     3181 ccatccagat agctgtacat gatcccccgc gcttcaaagc aggcccgaat aatggtcagc
     3241 atctgcacaa actgtgagaa taccaacacc ttatgtccgc tggccaggga gtcgcccacc
     3301 agttccagca gctgctccag cttagcactg gcctctggat cgggattgat atcgggattg
     3361 accagatggg ggtgattgca gatttggcgc agcttggtga gggcagccag cacgtggatg
     3421 tagttgaccg ctccggatgt ccctgcggga aacagctgct tttgcaccaa ctcgatcacc
     3481 tgcagataag tcttttcctg cagggacccc agtttgcacc aggagacctg ttcctgttta
     3541 tccggcagtt ccagcagcac ctctttcttg atccggcgca aaaggaaagg gctcaccatc
     3601 cggtgcagtc tttggtgggc aagatcgctg tcttcggcac cgatgaagtc atccctgaag
     3661 cgtttcaggc ttctcagata acccggcatc agaaagtcgt aaatgctcca gagctcggtg
     3721 agattgtttt ccaccggggt cccggacagg gccagacggt gttcgctgcc gagttttttg
     3781 atggcggcgg tgcgttgggc cgagacgttt ttgatgttct gcgcttcatc cagcacgatc
     3841 cagtagaaat tgatctgtct gagcagcgcg atatcattga gcaccaggct atacgagatt
     3901 agcagcagct tgacgttggg ctctctgagc atgtcttcgc ggatgctttt ccccccctcg
     3961 accaccagat aggggatgtt ggtatgaaac ttctcgatct cggcagccca gttgtagagt
     4021 agtgttttgg ggcaaatcaa caacgagact ctgccttccg gagtaccatg gatcacgctg
     4081 agggcctgga tggtcttccc cagccccatc tcatcggcaa ggatgccgtt caatccatag
     4141 tgctgcagca ttttgagcca ggcaacccct gccttttgat agccgcgcag gatggtctga
     4201 agattcactg gcagagccgg ggtcttttcc aaatgacgtc tgagcagatc agcggacatc
     4261 ttgtccagcc attcatcctg aatcagacgg atagcaggcc ggtccatcat caacctctga
     4321 taatacggca gttccaccaa tctggcgcga tagatggaat ccttgtattt ctcgctgcgc
     4381 gagatcaatc gctccatttc atcaaagact tcgcggttgc tgatgaagat cagacgcccg
     4441 tctttggtgt gcataaactc ttcattggaa cggaaaaagc gctttagctc ctcatgactg
     4501 aagctgtaat cccgatagct gtagcgcacg tcatagctga accagtcgat ctcatcaccg
     4561 cgccgggcat cgatctggac ggagattggc actttctgga cgaagaaatc acccaggtct
     4621 tccgcaatca ccaggtccca atcctcgtct cccatggaaa atactcgttc gcgcagggca
     4681 ttgatcgccg tgtcgccctg gaagctgatt tgggagtatt cctccactct gcggcggtcg
     4741 gcttcgggaa agcggcccat caggtctttc acctgttgaa agatccgcgg cggcagataa
     4801 aaccagccgt tgccctgctg actatccttg cgaaagtcgc agcgtacgag tgagctgttg
     4861 aagcgggtca ctgagagcgg tacttcctcg ccttccggat aacgcagcca gccttccatg
     4921 atcagcaggt ccccaccccg tttgagctcg agactgatcc tgggttcgga atccacgatc
     4981 tggggcagct cgatgccggg atcgaaatcc agatagatgt cgtgccgccg cagttcgtga
     5041 tgcaccacgg tgcggtaata gaccagatct ttggggctga tcagaagctg ccggcccatc
     5101 agcagttccg ttttctggtc tgaaaaagga aaccagaccg gatagacatg gtctctcatc
     5161 accagccagg tgggatatcc cgctatccag gccgagagtt catccacgat taccggcagc
     5221 accacaaagt tgcgtttgcc ggcaggttcg atcctgaggc tgagagggta aggctgtgta
     5281 tgcagtttga gcgtttcgcc ggtctccctg atcctgatca agtctccgca attcatcagc
     5341 aggctgagag cgcgtccgag atcacgtttg tagatggagt aaaacttgct tttgaagcta
     5401 taagcggatt tgttgagttg cagccagctc agaaagctca tgcggggacc gctgatggcc
     5461 cccaggttgc gcagatactc ttctttttga cgggcgggat agctttccag gctgtcccct
     5521 attgccatcc ggaccagata aggcagatcg atgggagcaa agctttcatg ataaaaacgt
     5581 actttatcgg ctcccggatc atagaggccc tccagttcca gcctggcgct ggacagagtt
     5641 tcgctccagt cctcggaaac cctcagatcg tcacgggaac aggtctccac cgcgggagaa
     5701 tcaaagatgt cggtcttgag atgatgataa gcgtagcgca gcaaagaaag aaaatggcgg
     5761 cagagctcct cctccccgca ttcagagcat tcgtgcctca cgatcagttg actgaccgga
     5821 tcatagacca ggtccagcct ctggcagaaa tgatgcctga cctccggtcg cggaaagaag
     5881 cgcaggtgat agagaccctg ctcgtccacc gaacgccagt attccagatc acctgcctcc
     5941 agaacgatga tggcatgatt aaaataccag gaagcagagt gctcgtgata gtctttgctg
     6001 aaaagacgtg ccattttacc tcggttattt acttaatccg ttcacccccg tcggataggc
     6061 ggattctgtc aatctatttc gttgatgttt ttgttttgat tggctatgta gcgggccgta
     6121 gcgtcggatt ccagttcggc gcggacttcc caacaagcct cagagaaagc ggagaaagca
     6181 gagaatacag aggttgtttc ttacaatgag aaatgagtat atgaggtttt ataacaatga
     6241 gatacgagat atgagaatga gaatatactg tgttgcgtat cgacagattt cagttcggcg
     6301 tctgacattc tttacgaccg caccctcaca gacggccccg gttctaaaca atcctctgct
     6361 tttctctgaa atcacctctg cttttctctg tgttttatgt gtttnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnntcacgg attgggaaaa atcttgtgcc agtcaggctc
     6541 caccatcccg tccagaccaa aaccgtgata ctggttttca tctccgctaa accacatttt
     6601 tgccccttcg cccgcgggat ttttggcttt ggcaacggcc atgatcatct tgtaaggtgc
     6661 cacatagaca tagttcgcct gattgagatc gttgagtttg cccatcattt caccggtgta
     6721 gatgtcagta ggcttccagg cgatcacaaa gtcccagcgt tcttgcgctt tcttctctat
     6781 cttggcatct tccaaggctt tggcgatgtc aaatccaccc gcgccgtcat gggtcttcca
     6841 ataattgtcc acattcagtt tcagggcttc cagcgccgtt ttggcctcgg cagacagctt
     6901 tttggcctgc atggccttta agcgcggcat tccaaagatt aggaaggcca ggatagccag
     6961 caccacgatt ccggctatga tccataattt cttccagcta aactttttgc ttgtttcgtt
     7021 ttccaatcta tcgctccatt tgattattca ccgcaagcgg cttcttctat cagatatact
     7081 aatggatatt ccgccgcgaa caatcagctt gtattgaacc tggaccccaa gccagagctc
     7141 atacaatctc gtcatgtttc aacgggacgg aagtttaggc catcttgaac gctgctactg
     7201 gccattctgc cccatcgtga tatatcatag aaaagctggt gtcagagtgg aagcctctcc
     7261 gcgatccatt tgagttaccg tatagaacct gaagtgttga gtagcgcaac ggtattgacc
     7321 aaattttggt gataaagcca gatatcatct acttgaattg ttctctgaac caaattacag
     7381 tattcgctgt tactctttta ttattctaag ttttaagccc ctcgctaccg agagtctata
     7441 gttgtatcgg cagaatatat tgtaataatt cttgacaata tatcaccagc cgctttttcg
     7501 tgtaacagac agtgtattag agaagctctt catttcacta aacaatatct atctatagag
     7561 gaagcaacat gttacaagat ctattttctg ctccggtcag gctgcgtgcc gggatttgtg
     7621 caggcagaac tgctatgttt ttggtatttt tcattttggc tatcacgggc acgggacttt
     7681 ggggaaccat ccaaacagta gtgtacaatg tcggcaacat ccctagcacc ttttgggtga
     7741 atccctccac caccaaaaga gcggaccaac caggcttact atccgtttcc ataccagtgg
     7801 gccagaagat agccagcatg gatattcagt attccatgtc cacagccaac ggcagctgga
     7861 tgagcgaaca gaattctttt ttgctgtgca gcaccaacgg cctcaccgaa gaagaagtat
     7921 attccggaga aggcgatacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 ccacagtttt cttaccctgc cagcggatgc ccaaatcctg gtgtatgaca tgggaaacat
     8101 tcccagcacc tacaatacgg acgttagtac atccagcaga gctgattcac caggtctgct
     8161 gtcggtaact atcccgccca acaaagagat aaaacacgtc ggtatccagt actccatgtc
     8221 cacagataac ggcagttgga tgagtgaaca gcggtctttc ctgctctgca gcaccaatgg
     8281 tgccaccgaa tccgaggtct gggagggaga gggacaaaga actggcattt gtgactatcg
     8341 gcgcgaccat cttaatctgg ccaggggact ttccggagta gtggatttcg aacttcacgc
     8401 tttccgcacc tggggaggca gtggaacaga cacagattat cagtatgtaa tgaatggtag
     8461 ctggcagctc atcattgagt tggaagaata tcccgttctc gattatccct cagatctgga
     8521 agccttcgat gtgggccctg gttctgcggt cctatcctgg gaagacaatt gctatcccaa
     8581 tgccacagag tgggatatca tgtatgaacc ctattcttat gaatttggga gcagcagatc
     8641 cctgatcagt aacgtcactt ccaatcccta tcttctcacc ggtctggtga gcggccagca
     8701 atatctctgg agcgtccgcg cgaacaaagg agacctggga gtaagcgact gggcagacga
     8761 tgagttcttt acaaccctct gggataccga agcaatcact tacaccctcg gtaatattcc
     8821 cactacgctc gtgcacccgg acagcatcca tgtcaccaac cgggctttgg aaccgggcat
     8881 catggaggtg acagtcccgc ccggtaagat gatctccggg gtggatttgg attatgcgat
     8941 gacaagcaac ggcagcggat ctgtgtatga tcagcggtcc ttcctcgtct gtagcaccaa
     9001 ttacggcaac gagcccgttg tgtttagcgg atcgggaagc tcaaacacct gttactactc
     9061 acgcgaagga ctccatctcg ccaacggcct ttccggaacc gttgtcttcg aactgcacgc
     9121 cttccgcacc cgctgcggaa ctggctcaaa cacggattac aattacgtga acaacggctc
     9181 ctggacactt acgatcaact acgcggaccg tccaccttca gacttatcag ccagcaatat
     9241 cggctacaac tccgcgcagc taaactggac agaaaactcg tttcccgctg ccccaacctg
     9301 ggatattttg tatggcccca tcggatttga tcgcctcagg tccggcaccc tgatcagcaa
     9361 tgtaagctcc cggccctaca cgctgtccgg tttgtattca tatgcgaatt acgaatggat
     9421 ggtgcgatcc cgcagcaatt ccgactgggt gagcgattgg tctgcccccc acacatttac
     9481 caccctgctg gaacctggcg atgtgatcct tacctataat ctgggagaca tacccactac
     9541 ttatgagata gctcccacca cctccagccg ggctttggag ccgggtttgt tgtcggtaac
     9601 ggtccccacg ggaaaaagga ttgaaaggat aggcatcagg tatatcatgt cagcgaatat
     9661 gggaggatac atttgtcatc agcggtcatt cctgctctgc ggcaccaatg gcgtaacgga
     9721 acctgaagtt tttgagggat cagttccagc ccagggaatg caatactacg gccgcgacga
     9781 tgtcagtatc gccaatggac tgagcggcgt agtggatttt gaactccacg cgttccggaa
     9841 ttgggggggc accggttcaa atacagctta taattacgtt tataacggta cctgggcgtt
     9901 gattgttcat tatgaggata ttcctgtggt gctcctggcc cccgtagtca cattctccgc
     9961 gccggcagga gtgccgcagc tcagttggac ggcagtgcca aacgcgaatt catacaagct
    10021 cttcggttcc gatgatccct acagcagcgc cccctggaga ttggtcacca tcacgccggc
    10081 caccactctc agctacacac cgtttctttc aggcacccag aagttcttcc gggtgcaggc
    10141 atcttctgat atgccctgag gacaagaggc aacccacgcc aacaagatcg gttttgtgag
    10201 atagcttgag taccaagcat actcaaaagc cgattagatg aaacttaaaa gcatcttatc
    10261 gacctctcat tgcatcgttt tctgatttta tgtcttgaca aatgcaaatt atcagataat
    10321 ttgtattcat agtcagcaaa ttttcgggag acaccatgcg cagaaagcaa gtaaccgaga
    10381 ttatcgctct ggtctacaca ccagagatca tcctccttac gggggccagg caggtgggca
    10441 aaaccaccgc gatgttacaa gttcacgagc agttgcgaag cactggcctg gatgcccatt
    10501 tctttactct cgaggacccg gattatctct tgctgctaaa tcagcacccg gatcagttat
    10561 ggagaatcgt ccccaaaccc atcgatggaa agaccgttgt gatcatcgac gagatccagt
    10621 atttggccaa gccctccaac ttcctgaagt atcactatga cctcaaccgg gataagctca
    10681 agctgatcgt cacaggctcc tcggcttttt atctggacga gcgcttcaac gactcgctgg
    10741 ccggccgcaa gcgagtgctt ctaatgccaa ccctttcatt tgatgaatat ttgcgcttca
    10801 agggagatac cagcctggca gaatgctttg agcaagctgc tatcacagaa ccggatttga
    10861 gcaatttaag catcccgcaa cagcgtgaaa ttttcagata ctgggatgat tacatcattt
    10921 atggagggta tcccgctgta gtgttggctt ccgattcaag agagaaagaa aggctgctca
    10981 acgagattct tagttcatac actaaacgcg attttctgga aagcggcata caagttcctg
    11041 ataaggccta ccaattgatg cggattttag cccaccagag cggcagtgaa ttgaatcgca
    11101 gcaagttatc agtagaactt ggtgtagcaa ggtcaagcgt ggacaccatg ctcagaatcc
    11161 tgcagaagag ctttcacgtc agcctcctga atcccttttt tggcggacat cctaaagaat
    11221 tgcgtaagat gcctaagctt tactatttgg atcacggcct ccgcaactgt ctatgcaaga
    11281 actatctctc tattgagggc cggatggaca ccggcaggct attcgaaagc tatgtgtatc
    11341 gacacttttt tgagaggaag ggtccagagg agctgcgtta ctggcgcaat cagcaacaac
    11401 aggaagtgga cttcatcgtt tccgagcagg ctgcctatga ggttaaatgg aacggcgatg
    11461 ctatccaaac atccaaatat cagagtttta gcaaactgca tccaaacctg cctctacgct
    11521 atattgtccg tcgcaatcct ggtgaagact gcctcctatt ctaaacagga atgctgggta
    11581 tttgattgac aaagcgcgcc acctaaaaaa actgacgcga taagaataga tggtgctact
    11641 aacaggtaaa agggaatccc gtgtaaatcg ggagcggtaa ctgccactgt aagcgacaca
    11701 taggcgggtt cggtcactga gctgacgagc ttgggaaggc cgggtccggg agcttgagat
    11761 catgagctcc agccgtcgcg agccaggaaa cctgccaccc agaccggaat tcctcacacg
    11821 gatttatgtg agctccggaa gtagttcccc tgactctatt gaagaaggag aactttatga
    11881 cccgcaagac attatgcctg atcattatga tcactctggc aagctgggga ctgcaggccg
    11941 cgattatcta cgcggtcaat tccgaatccc gcactttatc gagaatcgac accgatagcc
    12001 agagcatcga caacagcttt gcccagttgg gtttaacccc caaccttatg acccttgacg
    12061 aggatcacat ctgggtggtc tgcagtggcg acaacactat tcaggaattg gaccgccaga
    12121 ccggctccct gctgaggacc cttccggtag ccccatcctg caatccctgg gacgtcctga
    12181 aaattggcga ttatctctat gtgacgggcc tgtttaccga taaactctac aagctcagcc
    12241 tcgccagcgg aactgtggtg ggatccctcc aggtgggaac cgctccggaa gggctggcgg
    12301 cctatggaga cctgcttttc gttgccaaca cgggtggcta tcagaacaac tatgccaatt
    12361 cctcggtgag cgtggtcgat ctatctgatt tcagtgtggt aaacacaatc cctgtatgga
    12421 gtaaccccca gtatctgaaa gccctcgatg gccgcctgca tgtatcctgc accggaaact
    12481 ggagcgacct atccggcaag atcgacgtga tcgatatccc cagccaggag cacctggcca
    12541 ggatagatct gaacggacgg ctgatgtctg tgtggttttc atccgagggt atcgcactgg
    12601 tgggagaggc tttgagcact gggttctatc gctatgacgc gcacagcctg agtatcctga
    12661 acagcatttc aaatcctttg cagccaggtg cttacgccat cgatggcaat caggaactgg
    12721 ttgctctcat gaggcaaaac tggggaagcc cctcgctgct ctatattcgt catccggatt
    12781 tgagtgcctg gcagcagtac aacctgggcc tggtgtgcac cgatatcaag atttacgagg
    12841 cccccacagc cgtttctgat gatgcggtga cggctcccgg cctgaacctt tatcccaatc
    12901 cagccaggcg ctccgaactc ctcaagctgg ataacagatc caataaacgc tccgaactcc
    12961 ggctttacaa cctgcgcgga caaatgatcc acagccagat tctggctccc ggcctcaacg
    13021 aactaccgct cgcccctttg accttggctc cgggcctcta tttttaccgg tccggccttg
    13081 gaaaaagcgc tgtcaacggc agattaatca tcaggtaaat ccgtccttgc tctccttgag
    13141 ctctccttcc cctctctttt actcctccca tgccaaagca aaggagaggc aaaggagagg
    13201 cataggtggt gcaaaggagg ctaacaggca cggactgcta aacaggagta ctacatgacc
    13261 ttacagatca gaagggaaaa cccggaggat tacagcgcgg ttgaagccct cacccgggac
    13321 gccttttggg acgtccacgt ccctggttgt gacgaacatt atctggccca tatacttaga
    13381 gaagccgaat gcttcattcc cgagctggac ctggtggcca tttacgaagg tacgctggtg
    13441 ggcaacatca tgtatagcaa ggctttgctg aaattggatt ctggtggcgc gcttgagctg
    13501 ctgtgttttg gccccctcag tgtggctccc gcttttcaac gaaaaggcat cggtaaagct
    13561 ctggtggaga aatcattgga attggctggc ggcatgggct acggcgctgt gatcatctac
    13621 ggcgatccgg attattatcg ccgtttggga ttcaggccag gcgaggatta tgggatcaga
    13681 ggccaggaag gcatgtattc accggccctg caggtgctgg aactggtccc cggggctttg
    13741 aaaggcttgc atggcagctt tttcgaagat cagatctatc aactcgatcc ggaggccgcg
    13801 gccagatttg agcagggatt tccgccccgg gaaaaaggct tccggccttc tcaactccgc
    13861 tttcaggaga tgcttgccca ggcgcattac tgatctcctt gctcaatctc gatttcagag
    13921 gtagtgacat agccctgtca tataaccggg atattactga tggtagatcc accaaagtca
    13981 tatcaacgat agatgaccat ggaataaatc actttagggc gcctcaaagt ctacttttaa
    14041 aacagaggga cagagggaca gaggaatgga aagatcttgt aatgatatga catacaatct
    14101 attacagtgc tctgaatcct gatggtcctg tcatctggac atcttagttc cgtctgatct
    14161 gatactttat cagcctgaca accacgtcaa gctgttgccc agcgcacgga ccaattgctc
    14221 tcattggtca agacttctgg tataatatag agttagcctt ccagtccagc atcaggttcg
    14281 tgcttttttg agctggaaga ttattttgct tgcgttaaac aacgctttcc aggaatatgg
    14341 atttgtgggt gggtgcgttt gcatggcaag catctgcaga ctgtctgggg tgtcaaactg
    14401 acagctgagg ccttgcccac tcagacagag gaacttgctg agactacctc aaatcagcga
    14461 aggaagcgta gaatgcgcag agttttggtg ccgctgttgc tgctcatgct gagcggcttt
    14521 tccgcgtgcc gttccaccgg ccggatcatg acgcgcgtcc tttctccccg ttacttacag
    14581 cgcaccgatg atcccatcat caaggccgtt tttgctgatc tgagtggaaa cggacgcgat
    14641 ttactggtga cggccgaatc ctcccatccc gcttccgtca tcctgaaagc ctacgatcac
    14701 gacggcaggc tgatctttga acacgatacc cgtcaacagg tccgcgatat caaagcactt
    14761 gccaatccca aagacggagg tttctggata ttctataccc attcggagca agaaggtctg
    14821 ctgctgaagg ccgtgcggtt tgaccgggat gccgatgatc agcatcaaag caggagcttt
    14881 gaggccatcc cgatcttcac cacatcatcc gcttcgacca caggcaatgg aaatctccgg
    14941 ctggaaccat tgaacattat cgatctggat caagacggca aggcggaatt ggtctgcaag
    15001 ctcagctcca acattcctgg cgaagcccgt ggactggcag ttatggattt cggttccggc
    15061 cgtctcgagt ggtcattgaa gacggaaagc aacctcacgg atgtgctgat cgatgatctg
    15121 gacaacgacg gcagcgtcga gatcgtgctg gggtcccgtg tcgaacaggc ttccggcaac
    15181 gatgccgtcc ctttcaaccg gagcggagga tggctggccg ttttgggtcc cagaggcgac
    15241 ctcctctatt ccgaaaggca atttcagggg tctggggaag ttcgcctcgc gagcgtgaaa
    15301 cctgcccaat ctcccacccc tttgatcttc tgcgtcagta acacctgggg gccgcgcaga
    15361 acggtcaatg ctgtctcctc ccgcttttgg agcggcaaaa agctggtacc gacccgcgag
    15421 atttccacct ccaactcgtt tgaacgggct ggggaccttc ccaatctggc cgtcacggag
    15481 tttgatagaa aaccccggct gatcctgatc gaacggggaa ccgggatagt atcctatgat
    15541 gaagagctaa agaggacgat tcatacatgc ccggattttg tgaatgagat cctggacgtg
    15601 aaggatatcg atctggacaa caagcccgag atcatcgcca gacggcccga ccggactatt
    15661 ctggtgctgg atacagacct tcacaaactg gcggagctca gcaatccata tccaactgct
    15721 gacaaggtaa acgtttatgt ggtgggaagg ggcagtgggc ttgccaaagg cctggctgtg
    15781 tactcagagg agttggtgac ttattatggt tattatcccg tcaactggtt cacgcttctg
    15841 gggcggttta ccacggccaa ttcggtgtat tttgccatct tcatgctctt cctgctgctg
    15901 ctgatgattt ggtatgtgat ctataattgg cagtctatct atacgatcat gcccgctttg
    15961 cgagtcgcgg tgatctacct gcgcaatcat gaccggttga tgtattgcaa cccctatttc
    16021 ctggagctgg tcagagctcc ggtcgggcag aaaacgaggt ctctcaaggc cctctctccc
    16081 acccttggca agatgctgat cgatttcagg gcttcaggaa accggcttca aaatcacgag
    16141 ctggccctgg gtgaagacca gatggctggg atttaccggg tctcttttta cagagggcat
    16201 aatcccattt accgctacct catgattatc gtacctgttc ctactgtgaa cgctgcggaa
    16261 aagatcctct gggctgacac ggccagacgt ctttcccacc atgtgcgccg ccatatcacc
    16321 aacgtgatcc tggcacttcc gaaactcacg gacgaagaca tcgacagctc cgaacgcaaa
    16381 gagtatcatc agattatcag tgccgaagtg gaaaagatca gggtgtttac ccactccttc
    16441 cagcgctttt cagaactgcg agaccacgat ctgcgccaac acgacgtcct gccattggtg
    16501 gaacacgtgc tggaccacct ccacattcag gaaggcgtga agctgatccg cgcgattcct
    16561 ggctctcctt tacctgccta tctggacccc atccgctttg aggaagccct ggccaatctc
    16621 ctcaacaacg cgctggattc catgccctcg ggaggatatc ttcagatcac cgccaaaact
    16681 gtggcctccc aggagtctcc caaaagcgat ctgagggttc tgatcgaaat cgaagacaat
    16741 ggaaacggca tccctgaaaa gtatatggaa gagatctgga agcctttttt caccaccaag
    16801 caatcgggca cggggatcgg cctcccggaa agcaggaaaa tcatcgaatc gatgggaggg
    16861 atgatcacgc ttcacagcga agagagagtg gggaccacgg tctcgatctg gctgcgggtg
    16921 ggggaatcgg aaggctaacc tgacataaac atccaaaaca cctcattggc aagctgataa
    16981 tcatgtcatc tcttcatctg agttcaatca gttcagatgt agaaaatagt tctcgggtgt
    17041 gactgtctcg gtcagcgcct caggatacag gttcaaaaat gccctgaacg atccttgctt
    17101 cttaggattc cacttgattt cataggctga aagctgatca ttggccaatt ccagataatc
    17161 gatctcggca ccgttgcggt ttcgccagaa gtaactatgg tggtaaagta cccggttgag
    17221 cttgagcttg tgaaactctg agactatgta gttttcccac aacttgccga tatcgtcccg
    17281 caattccaaa gggcggaaat cggccaggat cgcgttacga atgccattat ccacaaagta
    17341 gattttgcgg gatcgcttga tttcgttgcg ctgattgcgg ctgtagtttg gcaagcgaaa
    17401 gacgatgaag gccctttcca ggatggtgat gtatctctca atcgtggtgg aatcactatc
    17461 cagaagctgt gccagttcat ggaaggatac ctcggaggcc agttgcaagg ccagggcctg
    17521 caccagttta tccagcagct ccggcttgcg aatgtcgtgg atgctgaata catccttgaa
    17581 taggtagctg ccacagagat tttgcagggt gggaactgcc agatgcgaat ttaggaccac
    17641 ctcaggataa gatccgtaga gcaagcggtt gggacgttgc tgcagttcgt ggatcatgct
    17701 cccgtctgct acaagctcct caaaggcaaa gggcagcagg ttgtattcga ttttgcggcc
    17761 ggtgagcggt tcattgagct tatctgccag ctcaaatgct gatgaacctg tagccacgat
    17821 gcggatatca ggcatggtgt cgtggatgat cttgagattg agccctgcgt tttcgatgcg
    17881 ttgagcctca tcgatcagga taagcttatt ctggcctatc acactcctca actgagctga
    17941 attctggctg gaaagcagag ttctgggctc ttgctcgtca cagttgaagt attggctctg
    18001 gccttgaaac tggtcctgga tcatattgag aagggtggtt ttcccgcttt gccggggtcc
    18061 caggatcagg ataatcttgt tcctgatcag ccactcgttc acagaatcga atatcttgcg
    18121 cttgatcata tgtttacctc atagacaaga taaatcacaa gccatacttg tcaaggaata
    18181 tatgcctctt ttcaggagta taactcgaaa tttacgcctc tttttggggt tatactccag
    18241 aaattacggg tttgacgcca aactcacagc gaagctctca cagcgaagca tctcacacgc
    18301 acagcatctc acccacagaa ttctattgac ataaagccct gccccaaaaa acttctatcc
    18361 ttagctacat gaatataata ccaaggagtt atgatatgga ttactttttg actgaagatc
    18421 agatcgagat gcgtgacctg gcccgccgca tagctatcga gaagatgaaa cccgtctcgg
    18481 aaaagtacga tgaagagggc attttcccct gggacatcgt ggaagtgatg aaacagagcg
    18541 acctttttgc catcctcatc ccggaagaat atgacgggat cagcggtaag gtggtagacc
    18601 tcgctgtggt cacggaagag ctctgcgccg tggatgcagg catagctttg gcttttggcg
    18661 ccaccggcct ggggatgtat cccatcttga tctccggtag cgaagagcaa aagcacaaat
    18721 acctcagtca aatcgccgcg ggagagcagc ttgccgcttt cgctctcacg gaagccaatg
    18781 ccggttccga tgccggggcc attgagacca ccgctcgtaa agagggtgac aactacatcc
    18841 tcaacggcac caaacagtgg atcaccaacg gaggcgaagc gggcatctat accgtctttg
    18901 ccatgaccga caaagaaaag ggcgcgcggg gctgctcctg cttcatcgtg gaaaaaggca
    18961 ctcccggctt cagctttggc aagaaagaaa acaagatggg catccgttcc tcggccaccc
    19021 gggaactgat ctttgaagac tgcgtagtcc cggccgccaa cctcatcggc cgtgaaggca
    19081 ccggattcat caccgccatg aaggtgtttg acaaatcccg tcccatggtg ggatctcagg
    19141 ccgtgggcat agcccggggc gccttcgagg ccgcggtgcg ttattccaaa gagcggcagc
    19201 agtttggcaa gccgatttcc agcttccagg cggtgcagtt tatgctggcc gacatggcca
    19261 cccagatcga ggccgccagg gccctcgtga tgcagaccgc ccgtatggtg gacagcgggg
    19321 ccaagaacta ttccaaagaa tccgccatgt gcaagtatta cgcctccgac gtagccatga
    19381 aagtgaccac ggacgccgtt cagatcctgg gcggatacgg ttatatgaaa gaatatcccg
    19441 tcgagaagat gatgcgcgat gccaagatcc tccagatcta tgaaggcacc aatcagatcc
    19501 agcgcggcat cgtggctgcc aacctgctca aagaatattg atcattgagg gccggggagc
    19561 tcccgctccc cggttgcttt tcaaaccagg ttttctgctt ctctctaagt cctaaacctc
    19621 aaaatggata taaaaatgga atggattaaa cacttccctc atgccgtcac cgttgccgat
    19681 cctgacggta aggtcacctt tatgaacgac aaggccatcc agaccttcgc ggatcatggt
    19741 ggagccgcat tgatcggcag cagtctgtat gactgccata aaccctcatc tcaagagaag
    19801 atcaaacaca tgctggccac cggagagagc aacatctaca ccatcgaaaa ggccggggtg
    19861 aagaagataa tcgtccagca acccctgttg gataacggtc gcgtgaccgg catcgtggaa
    19921 atttcgatca tcctgcccgc tgaggtagct cactttgtcc gaagttaaaa agctgctgct
    19981 gcacacctgc tgcgcgccct gtctgatcgc tccctatcag gaactcaagg ccaggggaat
    20041 agaggtcaca gccttctggt acaataccaa catccatccc tacacggaat acaaggcccg
    20101 caaagagacc ctggaagcat tttccgcatc tgaaggcttt cccctcatca tgcgggacga
    20161 atatggcctg gaaccctttg tgaaagcggt cgtttccgat atcgcggacc gctgcaggta
    20221 ttgctatgaa acccgcctgg ccgccactgc caagctggcc aaagagggag gatttgacgc
    20281 cttttccacc accctgctct acagccggta tcagaaacat gagttgatcg tcgagactgc
    20341 caggcgctac tctgatctgt atgaagtatc cttcttctat gaagactgga gaccgctgtg
    20401 gcacaaaggt atagagcttt ccaaagcgga ggggatgtat cgccagaaat actgcggctg
    20461 catcttcagc gaacaggaaa gatatctggg aggcaaataa tgagccgcta caaacagatc
    20521 gatctggaca acatcaaggt ctattccgtc aaagacaggc ccagcaaagt ggagccggag
    20581 ctgttcgcca aaccggcaaa ttcagacatg accggcttcc tggcatccct gcccaagctg
    20641 ttgaaagcac aagagttaaa cgagtttatc ggccacagcg tggaagccgc cagactgaag
    20701 aagggcttca tcatcggcct gggcggtcac gtgatcaagt gtggcctggt ccctctgctg
    20761 atcgagctga tggaacaagg gatagccacc tgttttgccg tcaacggcag cgtggcggta
    20821 cacgactatg aactggcaat gtttggcaag acctctgaag atgtggcggc caaactggca
    20881 gacggcagtt ttggcatggt ctatgagacc acctacggca tcaattccgt gatcaccaag
    20941 gcttcggaaa ctggcctcgg ttttggcgaa gcgctgggca aaaccatcct ggagaaggct
    21001 ccccatcctg aatactcatt gctggcaatg gcttacaaac accaaatccc cgtcaccgtc
    21061 catgtggcag tgggaaccga tatcctccac cagactccct gcgcggacgg caaagcgatc
    21121 ggagactgca gcctgagaga cttccgcatc ttctgcgaag aggtctcaaa gctcagcggc
    21181 ggagggattt atctgaatct gggttcggcc gtgatcctcc ccgaggtctt tcttaaagcc
    21241 ctcacggtgg cccgcaacat caaaggccgc gtgcaagact tctacaccgc cgtgtttgac
    21301 atgaacaccc actaccgcgc gatggaaaac gtggtgaagc gtcctgtcct caacggcggg
    21361 aagggctatt acttcatcgg gcaccacgag atcatgattc cgctctatat agcggcgatc
    21421 ctggagcagt ttgatctgga tatacctttt taacaaggat ttgagacccg ctacttcaat
    21481 cccgtgatct ggctgatccc ccaggccacc gagcgggcat aacttttcag gctggcgtag
    21541 cgcttactgc tgccagagtg gccttcgtct ttctgtagca gatagataat gcgggaatct
    21601 cccatgttat tgagccgcag cttctgggcc cacttgaggc cctcccaata gcccacccgg
    21661 taatccatcc agccactgct gatcagcatt tggggataga cctccggccc cacgttgtcg
    21721 tatggactgt aggacagcat atagctaaag tacttgggat cattgggatc gccccactcc
    21781 gcgtactcac ctaccgtgag cggcagatcg ggatccagca tagtgttgat cacatccaca
    21841 aaaggtacgt ccgcaatcac ggctttcacc ccggtgggga tcatattggt gacggcgccc
    21901 attaacaatc cacccgcact gccaccttcg atgagcaact ggcctggagt ggtcagaccg
    21961 ctggagatca gatattccac cgcgtcgcga aaatctgtaa agctgttttt cttgtggagc
    22021 agacgccccg cgtcgtgcca ggctttgccc atttcaccgc caccgcgaat atgggccagg
    22081 gcaaagatca cgcctctgtc caacattgat tggcggctgg aggagaacca ggggctttcc
    22141 acgtctccat aggcgccgta gccgttgagc cacactggct tgggaccctc cggcagctct
    22201 tttttgtgga tcagggtgag agggatccgg gtgccgtctt cggctacata gtagctggca
    22261 gaactggtat atttatcagg atcataaccc ttgggaagcg ctttggtata gatcagtgaa
    22321 tccgccttag tgtcaaaacc gtgctggtag atctgatagg gccgtagcca gctttcggaa
    22381 tagtagttga agccaggcgc gccaggctcc agattgttcc agaggcccag gtctgatggc
    22441 tgttctggga tgatctcatc cagcagcttg cctgtggagc gctcatagac ggccagttga
    22501 tcgtgaccgc cccgcatcgt ctccaccacg accttgtcat cgaacagaga aaagtcccag
    22561 atcgaagcac cctgaggggc agagatatac tgctgccaat cctctatggc ggaggcgttc
    22621 aggctgcagt gggcaatgga gtaatcggga ttccagaggt tggtgtggat atagagctct
    22681 ttatccagga tggaggctga gtattccagc tcgggacgcc ggcctgcaat ggcttgaaag
    22741 ctccccgtca gatcatccaa tggcatgatc caggcttcgg aactgttttt actgccagcg
    22801 gatagaaaca cccagctccg gtctccccat ctgtagagac ccaggttgaa agcaggatcc
    22861 ttttcctcat agagcagggt ctgctctctg gtgtccagat cgagccgcaa gcacttgtcg
    22921 ctgcgcatga tgtcgttttc agtgacgatg atggcgtgcc ggctgtccaa ctgccacaca
    22981 aagtccgcga tcctgtccag cttggtatca gtgattttac cagtgcctaa gtctttgagg
    23041 tagagacgat agacctctgc gccactcaga tcggccgtgt aagccagaac ccggttgtcc
    23101 gggctcacgg cactgaggcc cacatcaaag aactgcttcc ctttggccat cttgttgagg
    23161 tccaaaagcg tctccacctt tgctccggga gcgtccttct gtctcagata gacgggataa
    23221 tcctgtttgg aggtctggcg gttgaagtag taataaccgt tcaccagcgc tctggccgag
    23281 acatcttctt gggggatgcg gcctttgtat tctttaaaga gcttgtcagc cagctttttt
    23341 g
//
LOCUS       DAOSFN010000017        22862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1798_length_22862_cov_9.565440, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000017 DAOSFN010000000
VERSION     DAOSFN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 22862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22862
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1798_length_22862_cov_9.565440"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="PLO35_02280"
     CDS             complement(<1..1818)
                     /locus_tag="PLO35_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78519.1"
                     /translation="MSKRLLPAGLVLGLLIVSCGLNNTMYNARKYFRSAQARPLNSNG
                     KPTPQAIDEYTKTIQKCGVIITERKGSKIIDDALFLLARALYYKGNSAFQAKDQFEAL
                     IKGFPQSPHYPEAHIYLARVLRQINQPQEAEKLLESFVRDTRYLKFHPRALMVLAEFE
                     IGDKDYLRAQYWLQRIITDYPGTPEFKEAYFLFGKNYYEQKDFAASLTEFERLVETRG
                     ISKELKLEGRYYIALNHFELGDLERSGKIISALINDESRPDKLGQIRVLKARLLLAGA
                     DPQAGITEVDDLAKIYPRSMSSAAAYYYLGDHYFYRDGNIDQASTAYNKVRTEFPSSE
                     LVTASQAKVAALNQLKQNQSLSVTGNLQQFVDYHMLAAENYVTAFALPDSALMMYDRI
                     LQAAGPVRSKLDSLSIEALALQARADSLQIGITELETALTPAPVIPDSLSQPADKGET
                     TPADSIAAEPEASVQPSESTAQDSSAVIISEASVPDSVTQVEQPETSRETTPPTQPES
                     LEPNPASSIEAPDGEITDQPGSAIPDSVKTMESKEEETADPRGLELQQLRDSHTQTQT
                     DLASLDTRQKELEGILTRLDTEILPLVRISVSRRVRIPSS"
     gene            complement(1850..3379)
                     /gene="hutH"
                     /locus_tag="PLO35_02285"
     CDS             complement(1850..3379)
                     /gene="hutH"
                     /locus_tag="PLO35_02285"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOH78520.1"
                     /translation="MPTITIDGNSLRLEDVETIATGAARVELTPACLEKVKACRAFVD
                     KVISEGRIVYGLTTGFGKFSTVTIPPEHIAELQLNLIRSHATNLGEPYSKQQTRAIML
                     LRINVLAKGHSGIRPQTLITLVEMLNRGVHPIIPMRGSVGASGDLSPLSHLVLPLIGE
                     GEAEYQGKLMTGAEAMRAAGIEPVRLEAKEGLALNNGTQVMAALGVLGLLQAERLCLQ
                     ADIVAAASIDALKGTPRAFDPLVHALRPHPGQKASASNINKLLQDSPLRESHRDCGNV
                     QDAYSLRCTPQVHGAVRDALAYARGVLEIEINSATDNPLIFPEEDKVISGGNFHGEPL
                     AIALDYMAIAISELASISERRIEQMLNPSLNRGLSPFLAKRPGVDSGFMIAQLTAASM
                     ISENKVLAHPASVDSIPTSANQEDHVSMGSVSAIKYLQVAKNVCSVLGIELLISMAAL
                     DQRGLPSSPALEAVKSLVRKHIAALEEDRVMYPDLNKAIGLIDTAAVLQSVRNTGLDL
                     C"
     gene            complement(3508..4464)
                     /locus_tag="PLO35_02290"
     CDS             complement(3508..4464)
                     /locus_tag="PLO35_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOH78521.1"
                     /translation="MTSDKIILGISSCLMGNNVRFDGGHKYDDWLMETLGPWVEWVPV
                     CPEVECGLSVPREALRLVGNPEAPRLVHVKSGIDQTQRMIDWSAPRIEGLKAKKLCGY
                     VFKSKSPSSGMERVKVYPEKGGAARKIGVGIFAREFMKAFPLLPVEEEGRLHDPVLRE
                     NFIERVFVMKRWNDLIETGINPGAMVEWHTRHKLMIMAHSVPHYRSMGKLVAAIKDHS
                     PQAFASAYLEQLSQALRKPATPSKHLNVLQHIMGYFKQELSPWEKAELLDIFSMYKDR
                     HYPLIVPVTLLNHYVNKYDKAYLKAQHYLNPHPVELNLRNHS"
     gene            complement(4561..5961)
                     /gene="thyX"
                     /locus_tag="PLO35_02295"
     CDS             complement(4561..5961)
                     /gene="thyX"
                     /locus_tag="PLO35_02295"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOH78522.1"
                     /translation="MRVIIAGYNVDSSLLHNLDKDLATPEVLSAAYARISRSVKTASE
                     LRIEALREIAKARTSNQKIIFEMGHASVAEHAVFNIDLIGISRCLTEQVQRSRLASFT
                     EKSQRYVTFDRDYVVPAELDATEALKHEYTRLCDLLFEEYALSLDTLVEQLAETHPQL
                     GKRDLETMAKEDARYILPLATKTQMGMTINARSLEVLLRRLSSLSTNEAKLLWDKLYS
                     QVSAISPSLIRHTNAQPGEFCLESVVPKLSDQPHRWAKQLPSSTLLSSTAQADELILA
                     ILLFEQGHGDPQTWQLHLSRLSDSAKSGLWQRLFQDLKPWQKLPRAFEAMELDYLLEL
                     SECAWGQLKRHRSATLIKSSHIDSGGPLVIPPSIQISGRQDRWQELASQVFAFRDKLA
                     GPLRPYLRINAEAVKVYARLNLRELYHFSRLRSDEHAQWEIRQLSDVMVSLAKASAPN
                     ATAFLGGKSSFDSLIR"
     gene            complement(5968..6741)
                     /gene="deoC"
                     /locus_tag="PLO35_02300"
     CDS             complement(5968..6741)
                     /gene="deoC"
                     /locus_tag="PLO35_02300"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOH78523.1"
                     /translation="MNKIEAIARRLFPNNELCRCGGDHVICLQCAVCRADQPDQLWNP
                     SQGIAAVIDATVLKANASQEEVNTLCEVANDHHCASVCVNSWFSHIVRLRLANPVKNC
                     TVINFPLGAGCLEAVVEEAKAVIQHGVQEVDMVQSLSAIRSGHPKLSYDIVKAVAEVC
                     RKEEVRLKVILETCYLSEDEIIASCLYAKKAGADFVKTSTGFGSAGATVEHIKLMRQS
                     VGPKLGVKASGGIRDRATALAMLSAGANRIGASSVQALI"
     gene            complement(6812..7927)
                     /gene="rlmN"
                     /locus_tag="PLO35_02305"
     CDS             complement(6812..7927)
                     /gene="rlmN"
                     /locus_tag="PLO35_02305"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOH78524.1"
                     /translation="MLNSLYGIRPSDLGALIIKDFPAFRTKQLLSWVYQKMVFDPDHM
                     SNLPKPFKAWLNEAFDLSMPEIVDRQISRDGSIKLLLKLKDGLEVEAVLIPEGKKRTL
                     CVSSQTGCARACAFCATGTLGPGRNLLPHEIVQQVLLASQILIQQNASDGSESEAPDG
                     TDHLTNIVFMGMGEPLDNFDNVMEALRIIQDPAGLAFSPRRITVSTCGIVPGIIRLAD
                     MGVKVKLALSLNSAVDSIRDKLMPVNRLYPLPQLKTALQYFKGKNPFRITFEYILIPG
                     VNMDETAIKALRRFTGDLASKINFIPFNPVPQLGYRAPTKAEVESFMAAAASLPQAIT
                     LRKSKGQDINGACGQLALNHLSTKQVEKVERVKKAER"
     gene            complement(7921..8760)
                     /gene="ricT"
                     /locus_tag="PLO35_02310"
     CDS             complement(7921..8760)
                     /gene="ricT"
                     /locus_tag="PLO35_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOH78525.1"
                     /translation="MQDYIEVEFRTGRLGYYQNSSGLPIQPEDLIIVEVERGDDIAQV
                     VHLSINSTEIAASVQGPRQYHIKRRASDEDIEKMGNLSYEEEKAAKTFMDILERYPFT
                     MKLIDTVYQFDGNKLTFFFSAEGRIDFRVFVRELANVFKTRIELHQTTGRDEAKRLGG
                     FGMCGNQYCCGSFLKRFNQVTIKMAKDQNLAGNLSKISGPCGRLLCCLNFEEDFYVEE
                     SREFPTLGTCVMLKGKKMYVFKNDVLNKRVHLTDEDQLLTELPLEEYQQLTVVEVPDL
                     EPC"
     gene            complement(8761..>9616)
                     /locus_tag="PLO35_02315"
     CDS             complement(8761..>9616)
                     /locus_tag="PLO35_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78526.1"
                     /translation="VKEAEGKGMLFRHLADLVHASPELIRQDFEQFFYDKDNAFETEP
                     GVTVLDPYSVWIGEDVTLKPGVVLDASGGPIVIDEGAVVMPNAVLMGPCYIGKKSQIK
                     IGAKIYPGTSIGPVCKIGGEVEDSIFQAYSNKQHDGFLGHSFVGEWVNLGADTNNSDL
                     KNTYANVDFYSYEAGQKIDSGSMFMGCVIGDHSKLGINCSINTGTVIGVGANLWGSPL
                     IDGFIPDLSWGMANDLKSYRVPAFLWTANLVKERRNLQLGKAERELYLEIARSRQDLA
                     ASKTSNGV"
     assembly_gap    9617..9716
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9717..9823)
                     /locus_tag="PLO35_02320"
     CDS             complement(<9717..9823)
                     /locus_tag="PLO35_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOH78527.1"
                     /translation="MIIFDDQPIAYYPLALTRSTGDLRCGILKLRQRLQA"
     gene            complement(9820..10974)
                     /locus_tag="PLO35_02325"
     CDS             complement(9820..10974)
                     /locus_tag="PLO35_02325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH78528.1"
                     /translation="MIHSILYRVLILLIASGFVTYIVHCIRFYWGYKNYQPLLSFKKR
                     TVSIVVVCHNEAANLPTLMTCLVNQKYPRELYEIVVADDDSTDDTEQIIRSYEDSGVP
                     VHYIKVKGREKVVSPKKQAMEIAIKAAGGELILTTDADCVVPLTWVSSMVEAFTDDVS
                     MVAGYSRTQIRDWGTASLLHKYEHFDFAATYLVLGGGYTLGKSWACIGQNLAYTKAAW
                     ESVGGFDKIRHLISGDDVNLMQLMRRSGHRIIFNFNPQSFVYTHPVPSWKRLVNQRSR
                     WASNMKYQLTFNPEFFFILLSMAFMYWGGPLMLIFNWQLALAVFAFRMLIEITFLSYA
                     RSRFGVTARMMRFYPIWMVIQTFFLVFTMVLGQFNLFIWHGKRPPKGARV"
     assembly_gap    11011..11110
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11173..13227)
                     /locus_tag="PLO35_02330"
     CDS             complement(11173..13227)
                     /locus_tag="PLO35_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOH78529.1"
                     /translation="MIYRDRKTLMRILGYLGLGFCVLTGIVLGLFWFYRDDLPPTAEL
                     RNFVLRTGSEVYDRNGRMIYLFAFEKRKLVSVKELPPHLIDALVVTEDKRFYRHFGID
                     PFSFVRAVLVDIRTRDFSQGASTITQQMARNMFLTLDKQVSRKMKEAILALRIEATFS
                     KEEILEIYFNKIFWGGQIHGVETAALYYFNKHARDLSIAESAMLVGMIQRPNYYDPIR
                     KPENAKRRRDHVLERMFKANKISEEDYAIAVNTPVKPQAGSMRRFASDYFIEHIRTYL
                     EKKYGTERLFEGGLRIYTTLDPDISGVADSVLNNYLANIENSSGHRNKYSHVPAGAQD
                     IDTQYIQGGLVLMENKTGFVRALIGGRNFEHSKFNRMTQAKRQPGSSIKPTFYTLAIE
                     KGYTPSTIISDSPFTLGNWSPQNAGRNYHGYTRMRVALTHSYNIWAVKCAYDLGLDVV
                     NEAFKRFGLNRRAENLTAALGSHEIIPIDLISGYTTFPNDGMRVSPVFITKVEDTSGR
                     ILERGTGQKHRVCSPEVAYIMTSMLESVFKTGTGAGARSGYSWPGAGKTGTTSNNYDS
                     WYIGFNELFTLGIWTGFDNSNLFAGKAQASNMWGRIMAQAIKIDNNGRYPKADDPRYA
                     FNVPERIVKRSINPYTGFTTNGGGIEEVFIEDNIPPAVADTLQFNFYPTRWGYNDRLE
                     TR"
     gene            complement(13217..13747)
                     /locus_tag="PLO35_02335"
     CDS             complement(13217..13747)
                     /locus_tag="PLO35_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOH78530.1"
                     /translation="MSSVKPRPLIFLAFVTAIACSVHIVESLVSRSLPLPFLRLGLSN
                     VVVLYLVMEKQVWQAVAVNITKSLIGGVATFTFLTPGTILSLGAGLAAIAIMELARRS
                     RLGFSIYGISLAGATAHNLGQLLIIKWVVLHQRGVFMLTPILLFLGLISGLLIARIAQ
                     AVFPRINELRLVNNDL"
     gene            complement(13744..14418)
                     /locus_tag="PLO35_02340"
     CDS             complement(13744..14418)
                     /locus_tag="PLO35_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH78531.1"
                     /translation="MKDLSFTLPDGHNLIVLGRSGSGKTVLSKTLMGFYMPTAGSVTI
                     DGRDVYADPDSGNESTQDQFAIVFQNAALLDSMSVFQNVALPLYTRGEKDTSQVRQKV
                     QKVLDEVGLGDTMEKFPSELSGGMRKRVGIARALVYNPRYIVFDEPVSGLDPITSDEV
                     LYYINRIVSSKTATTITITHEVRDLERVGDMVLFLESGESRYFGPLNDLPSLPDPLIR
                     KFFGLP"
     gene            complement(14444..14821)
                     /locus_tag="PLO35_02345"
     CDS             complement(14444..14821)
                     /locus_tag="PLO35_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOH78532.1"
                     /translation="MSVKKPGTTSLVLTLLWWALIWILSSVPSDDLPSLQILSIDKLA
                     HIGVYFVLGLLVNSWLKTRQIKASKRSLIYLALLCSALLDEYHQTFIPGRSVSIYDFL
                     ANALGLGFAWTAGFLNRDQRPES"
     gene            complement(14818..15543)
                     /locus_tag="PLO35_02350"
     CDS             complement(14818..15543)
                     /locus_tag="PLO35_02350"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOH78533.1"
                     /translation="MKIQISKHWLPAILMLVLCLSLQGKRMSYTVKAMGLSVANLIIE
                     RDPGASLVSVKTKSLITNALFPSIDNEYTIRYSSGYLPLKYNRAVRQNKLSDNVLTVY
                     DHSKSLAAESHSRNGETITYKINPATRDFFSFIAMLSDGKAGAGNYELDANGSLWKVS
                     VSYLGNKVIKTAAGKFSSRGYKLSLSSATHSKAPYVDMVTHNLFQKGSKVELWVDGKG
                     LVVKASMKKGAIGSSWELKDITP"
     gene            complement(15524..16795)
                     /locus_tag="PLO35_02355"
     CDS             complement(15524..16795)
                     /locus_tag="PLO35_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOH78534.1"
                     /translation="MDEGPAFTLIGLLFLSAFFSGSETAFFSLSRLYLKKMEGFGTPG
                     AKRALTLLKKPRRLLITLLLGNTFVNMAISSLATLTVIGIAEKYSSWSLSSLVTVQII
                     LTTVVILMFGEIIPKLLALSTASAFARTTSMPLQILQYLLFPLVWVFERLSMLVSRKQ
                     GADHYPGQSITSEEFHNLIHSESSNHSLEDHEKQMLVGLFRFREAQISEIYVPRVKIS
                     AIDEDASLEELRDLILETGYSRIPVYRETIDDIIGIVYVKDMLLYPEKTSITEIMRPA
                     WFVTENMKVQALLNQFKTRKMQLAVVVDEYGGTSGIISLEDILEEIVGEIHDEYDHEE
                     APEITAHSDNHFTVSGSFNIRQFNHQFSTDIDPEEFDNLAEYLLSHFNHVPQSGESHT
                     LDGRVKLTILDSDGKSIKQVEVITLNEDTDI"
     gene            complement(16797..17237)
                     /gene="ybeY"
                     /locus_tag="PLO35_02360"
     CDS             complement(16797..17237)
                     /gene="ybeY"
                     /locus_tag="PLO35_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOH78535.1"
                     /translation="MLEVINESGVEIDPLPLIRIAELICAEDIPDQVAELTLLICDSK
                     RMRQLNKTYRGKNEDTDVLSFLSDSIPSEQAVRGLKCLRGDIVVDINQADRQKGFHSL
                     ETELQYLLIHGLLHLLGHDHIKTQDKEKMEHKETYFRYKLIGAC"
     gene            complement(17230..19350)
                     /locus_tag="PLO35_02365"
     CDS             complement(17230..19350)
                     /locus_tag="PLO35_02365"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOH78536.1"
                     /translation="MSSKAFIFSLITALALAALYHVFAVGRFSYPEYRLTVGQVADFE
                     LIAPFDFPVLKSEKQLKLDRDLALEQLEAPFSVSEQVRFDSFSKLDQLFTLLSEYPED
                     SQREQLRAALNGLGADPDPAFTALALNPDSRERAYEYIYESISSVYKKPVYENILSDS
                     ITVLSDEQLRRANRDSFQDLEAAREAFRSRLAERGYGGFALWLSKFLIEADLVVNQAK
                     FEELKQLSVSAIPASEGEVLQNEIIIRKNSRVTETDINKLKSLTEAYKSRDVRRSPWE
                     QMGLSLGMLLYMLMILVIASYALPTLVPKDSAQEVSQFPINLSFVFLAVLAVINHQIL
                     GLNNILIPFALITLSVATLLGFEFGIFLTICNAMVLNPFVNWESYTPVLLLLSTLIAL
                     VLIRRLGAWHEYIRMWAALFASTLAVNLALALYKSDPLLTILRNSGYALISSILSVLG
                     AAAVISFFERRWNRSTKQALLELLDFNHPLLKKLATQAVGTYHHSLVVGNLAERAAEA
                     IGANPLLARVGSYYHDIGKVINHEIFTENNAASSEIHDLLDPQESARMIRDHVKEGII
                     LASRYHIPPDVIDIIKEHHGSSKIRYFLDLAERRGSDPNPEDFSYPGPKPLSKEAALV
                     MLADIVESTTKAKNISSRAELEKIIDTTVQRLIWEGQFDEAPITIKDLFKAKQTMLPV
                     LESIYRKRLDYPDENPKPEAPANA"
     gene            complement(19401..20741)
                     /gene="radA"
                     /locus_tag="PLO35_02370"
     CDS             complement(19401..20741)
                     /gene="radA"
                     /locus_tag="PLO35_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOH78537.1"
                     /translation="MAQSFFCSDCGHETSKWSGKCPACGSWGTMKESSGVSGKKGGAT
                     RPAREKGSPERIIDIKTDAISRIATGINELDLVLGGGIVSGMLTLIGGEPGIGKSTLM
                     LQLSQWLGAMDKKVLYVSGEESDQQIRLRASRLDVNSPNIWLYCTNDVEAIIETVDQT
                     SPDFVVVDSIQSVSIPSLDSIPGSVTQLRESCNRLARTGKTLGLPIFMVGHVTKEGLV
                     AGPKIIEHMVDTVLYFEGEARGQYKILRAVKNRFGSTNEIGIFEMTNTGLREVSDPNH
                     IFLSRDPALSGTSIGCVMEGTRSFIVEVQTLATGSNYGTPQRVAVGLEQKKLAMLLAI
                     LEKNLALYLRSSDVFVNLAGGMRSGDTGLDLAILAAIISSLKDTPLPEKAVFIGEVGL
                     NGEVRPVSQTDSRVNEAVKLGYEQVYVSAHSKVKARKGVIKIKDIKAVYALFKE"
     gene            20924..21169
                     /locus_tag="PLO35_02375"
     CDS             20924..21169
                     /locus_tag="PLO35_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOH78538.1"
                     /translation="MKPNIVIFGTPTCAWCKKVKEYIKSRGFTFKYVDVSTDGSALRD
                     MVRKTGQQGVPQLWIDNVAVVGFDRAKIDNLLSKTNK"
     gene            21186..21641
                     /locus_tag="PLO35_02380"
     CDS             21186..21641
                     /locus_tag="PLO35_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOH78539.1"
                     /translation="MSTSAENFHNLITRLQVVLSEIDYAQKACLQAGKMECQLLNHLY
                     LVKTPVNMNELAKVLGVSHSRVTRIMDNLVSKKLVTRRPSEEDRRCWFAIITDKGRKL
                     AENSQQTVVDQQKKIIAQLPAKEVDNIFKALKAYVEQYEAVLKATYVEL"
     gene            21641..22069
                     /locus_tag="PLO35_02385"
     CDS             21641..22069
                     /locus_tag="PLO35_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOH78540.1"
                     /translation="MTTKASMKWVKDMLFDAEITGHHILMDADTQWGGKDQGARPKPL
                     LLAALSGCSGMDVVSILDKMKVPEYKFDVDMEADSTTEHPVTYHTIRMKFLFEGQDLP
                     KDKFIKAVKLSTERYCGVMAMLKKAANIIVNVYINGEEVN"
     gene            22069..22620
                     /locus_tag="PLO35_02390"
     CDS             22069..22620
                     /locus_tag="PLO35_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOH78541.1"
                     /translation="MKHVFLLILIMSLSLTGCKAKDTQELNPEAQNNPEAQNAPELGN
                     VLDPNVAADNTDSPTWLVSFDQALAQAKARNLPVLINFSGSDWCSWCIKLANEVLNKP
                     EFLSYAKDNLVLLNLDFPRKTQLPESQAKANEAIAQRFKITGFPTVILLDSQGEKIGQ
                     TGYQPGGPQKYIEHLKQIMNPSK"
BASE COUNT         5257 a         5788 c         6150 g         5467 t
ORIGIN      
        1 actggaaggg atcctcaccc gcctggacac cgagattctg accaggggca gaatctcggt
       61 gtccaggcgg gtgaggatcc cttccagttc tttttgtctg gtgtccagag atgccagatc
      121 cgtctgagtt tgagtatggc tgtcgcgcaa ttgctgcagt tccaagcctc tggggtcagc
      181 ggtttcttcc tctttggatt ccatcgtctt gaccgaatcg gggattgcgg agcctggctg
      241 gtctgtaatc tccccatcag gtgcttcaat agacgaggct ggattgggtt caagggattc
      301 aggctgggtg ggcggggtgg tttccctgga tgtctctggt tgctccacct gagtcacaga
      361 atctggaaca gaggcctcgg aaatgatgac agcgctgctg tcctgggcgg ttgactcgct
      421 gggttgaaca gaagcttcag gctcagccgc aatgctgtcc gcgggagtgg tttcaccctt
      481 atctgcaggc tgcgaaaggc tgtctggaat tacgggagca ggggtcagag ctgtttcaag
      541 ctccgtgatc ccgatctgga ggctatccgc tcttgcctgc aaggccagag cttcgatgct
      601 caggctgtcc agtttggatc tcacaggtcc ggccgcctgc agtatgcggt catacatcat
      661 cagagctgaa tctgggaggg caaaagcggt gacatagttc tctgcggcca gcatgtgata
      721 atccacaaac tgctgtaagt ttccggtcac agagaggctt tggttttgct tcaattgatt
      781 gagggcggct actttggctt gggatgccgt caccagttcc gagcttggaa actcggtgcg
      841 gaccttgttg taagcggtgc tggcttggtc gatgtttcca tccctgtaaa agtaatggtc
      901 acccaggtag taataagccg ccgcggaaga catgctgcgc ggataaatct ttgccaggtc
      961 gtcaacttcg gtgatgcctg cctggggatc agctccggct agcagcaagc gggctttgag
     1021 gaccctgatc tggcctagtt tgtcgggacg ggattcatca ttgatcaggg ctgaaatgat
     1081 cttgccgctg cgctccaggt cgccaagttc aaaatgattg agggcgatgt aataacggcc
     1141 ttccagcttg agttctttgc tgatgccacg cgtctctacc agcctttcaa actcggtcag
     1201 agaagcagcg aagtctttct gttcgtagta gtttttgcca aaaaggaagt aggcttcttt
     1261 aaactcgggg gtgccgggat agtctgtaat gatgcgctgc agccagtatt gggctctcag
     1321 atagtctttg tctccgattt caaattcggc cagcaccatc aaggcacggg ggtggaattt
     1381 tagatagcgg gtgtcgcgga caaaactttc cagcagcttc tctgcttcct ggggctggtt
     1441 gatctgccgc agcactctgg ccagatagat atgggcttcg ggatagtgag ggctctgagg
     1501 aaaacctttg atcagggctt caaactggtc cttggcctga aaagcgctgt tgcccttgta
     1561 gtaaagggcc cgggccaaca gaaagagcgc gtcgtcgatg atcttgctgc ccttgcgctc
     1621 ggtgatgatg accccgcatt tctgaatggt cttggtgtat tcatcaatcg cctggggagt
     1681 gggcttgccg tttgaattga gaggacgcgc ctgggccgat cggaaatatt tccgggcgtt
     1741 gtacatggtg ttgttgaggc cgcaggatac gatcagcagc cccagaacca atccggcggg
     1801 aagcagtctc ttgctcatga agggaaggct gtggaggccg gaaatcttgt caacacaaat
     1861 ctaagcccgt gttcctgaca gactgcaaaa cagcggccgt gtcaatcaat ccgatcgctt
     1921 tgttgaggtc gggatacatt acccggtctt cttccaaagc ggcaatatgc tttctaacca
     1981 gtgatttaac ggcctcaagg gctggcgagc tgggcaggcc acgctggtcc agggccgcca
     2041 tcgagatcag cagttcgatg cccaacacgc tgcagacgtt ttttgccacc tgcagatact
     2101 tgatggctga tacggaaccc atgctcacat ggtcttcctg attggcggat gtgggaatgc
     2161 tgtccaccga ggcgggatgc gctaaaacct tgttttcact gatcattgag gcggctgtga
     2221 gctgcgcgat catgaagccg gaatccaccc ccggacgctt ggccagaaaa ggactcagcc
     2281 cgcggttgag agaaggattc agcatttgtt ctattcggcg ctctgatatg gaggccagtt
     2341 cgctgattgc gatagccatg tagtccagcg cgatcgccag gggctcacca tggaagttgc
     2401 cgccggagat cactttatct tcctccggaa agatcagggg attatcagtg gcgctgttga
     2461 tctcgatctc caaaacgcca cgggcgtagg ccaaagcgtc tctcacggcc ccatggacct
     2521 gaggtgtgca gcgcaaagaa taggcatcct gcacgtttcc acaatcccgg tgagactccc
     2581 ttaatggact gtcctgcagc agcttgttta tgttggaagc gctggctttc tggccggggt
     2641 gaggtctgag agcgtgcacc agcggatcaa aagctctggg agtacccttg agagcgtcaa
     2701 tgctggccgc ggctacaata tctgcctgca aacaaaggcg ttcagcctgc agcaaaccca
     2761 gaactcccaa cgctgccatc acctgagttc cgttgtttag cgccaggccc tccttggcct
     2821 caagtctgac aggttctatt cctgccgccc gcatagcttc cgccccggtc ataagcttgc
     2881 cctgatactc cgcttcacct tctccgatca gaggtaagac caaatgggac aagggagaaa
     2941 ggtctccact ggcccccacc gatccccgca ttggaatgat gggatggaca ccgcggttga
     3001 gcatctctac cagtgttatc agcgtctgag gccggattcc cgaatggcct ttggccaata
     3061 cgttgatcct gagcagcata atggcgcgtg tctgctgctt gctgtaaggc tcgccaagat
     3121 tggtggcatg gctgcggatc aggttcagtt gcagctcggc aatgtgctct ggggggatag
     3181 tgacagtgct gaatttgcca aaaccggtgg tgaggccata tacaatgcgg ccttcagata
     3241 tcactttatc cacaaaggcc cggcaggctt ttaccttttc cagacaggcc ggggtgagct
     3301 caacgcgggc cgctccagtg gcgatggttt ccacatcttc cagtctgagg ctgtttccgt
     3361 ctatcgtgat ggtgggcatg agactccttg atataacgcg ctgaacgcga tcgatttcaa
     3421 tgttttttgg gccattaatt tgagaggggg ctttttgtca aatgcttaac aaagatagca
     3481 gatcaataga gaaaacagac tcagctacta agaatgattg cgcagattca gctccacggg
     3541 atggggattg aggtaatgct gagctttcag atacgcttta tcgtatttgt ttacatagtg
     3601 attgaggaga gtgacgggca cgatgagagg atagtgccga tctttataca tagagaaaat
     3661 atccagcaac tcagcttttt cccaaggact tagctcctgt ttgaaatatc ccataatatg
     3721 ctgcaggaca ttgagatgct tgctgggagt agcaggtttt cgcagagctt gcgagagctg
     3781 ttccagatac gcagacgcaa aagcctgagg gctatgatct ttgatggccg ccaccagttt
     3841 tcccatggac cggtagtggg ggacgctgtg ggccatgatc ataagtttat gacgcgtatg
     3901 ccattccacc atcgcgcctg gattgatccc agtctcgatc agatcgttcc agcgcttcat
     3961 cacaaaaacg cgctcaatga agttctctct caagaccgga tcgtgcagtc tgccttcctc
     4021 ttccacgggc aggaggggaa aggccttcat aaactcgcgg gcaaaaatcc ctacaccgat
     4081 cttgcgggcg gcccctcctt tttccggata gaccttaact ctttccatgc cgctggaggg
     4141 ggatttgctc ttgaacacat atccacagag cttttttgct ttgaggccct ctattctggg
     4201 cgcgctccag tcaatcatcc gctgagtctg atcaatgccg cttttcacat gcaccagacg
     4261 cggagcttca ggattgccaa ccagcctcag agcttctctg ggaacgctga gaccgcattc
     4321 cacctccgga cagacgggca cccattccac ccagggtccc agggtttcca tcagccagtc
     4381 atcgtattta tggccaccgt cgaagcgtac gttattgccc atcaggcaag aacttatgcc
     4441 aaggattatc ttgtcgcttg tcatatcata ccaccttaat tattgaatca catataatag
     4501 tgtatgctca aagctacgct aggtgaaagg aaacttagtt gatcagtaga gctggaagca
     4561 ttatcgtatc aggctgtcaa agctgctctt accacccaaa aaggccgtcg cattgggagc
     4621 agaagctttg gcaaggctta ccatgacgtc agaaagctgc ctgatctccc actgggcatg
     4681 ttcatcggag cggagacggc tgaaatgata tagctcccgg aggttcaatc tggcatatac
     4741 ttttaccgct tcggcgttga tgcgcagata tggcctcagg ggaccagcaa gtttgtctct
     4801 gaacgcaaaa acctgggatg caagttcttg ccaccgatcc tgacgtccgg agatctggat
     4861 cgagggagga atcaccaaag ggccgcccga gtcgatgtgt gaactcttga tcagggtggc
     4921 gctgcgatga cgtttgagct gtccccatgc gcattcagaa agctccagca ggtaatccag
     4981 ttccatggct tcaaaggcgc ggggtagttt ttgccagggt ttcaggtctt gaaagagccg
     5041 ttgccacaag ccgcttttag cactgtcact gagtctggag agatgcagtt gccacgtttg
     5101 ggggtcgccg tggccctgtt caaagagcaa aatggctaaa atcagctcat ccgcctgagc
     5161 tgtcgatgaa agcaaagtgg aggagggcag ttgtttagcc catctgtgcg gctggtctga
     5221 aagcttcgga acgacgcttt cgaggcaaaa ctcgccgggc tgggcattgg tatggcggat
     5281 cagggacgga gagatggcgc ttacctggct gtacagctta tcccacaaca gcttagcttc
     5341 gttggtagat agcgatgaca gccttcttaa cagcacttcc aggctgcggg cattgatcgt
     5401 catgcccatt tgagtcttgg tagccaatgg caaaatgtat ctggcatcct ctttggccat
     5461 ggtctcgaga tcgcgcttgc ccagttgagg atgcgtctct gccagctgtt ccaccaaagt
     5521 gtcgaggctg agagcgtact cttcaaaaag caggtcgcaa agccttgtgt attcgtgctt
     5581 gagagcttct gtcgcatcca gttccgcggg gaccacataa tctctgtcga aggttacata
     5641 gcgctgggat ttttcggtga atgaggccag acggctacgc tggacctgct cggtgaggca
     5701 tctggatatc ccgatcagat cgatattgaa gacggcgtgt tcagccacgg aagcgtggcc
     5761 catctcaaag atgatcttct ggttggaggt cctggcctta gcgatctcgc gcagggcttc
     5821 tatcctcaat tcggaagcgg ttttaacgct acgactgatc ctggcataag cggctgatag
     5881 cacttccggg gtggcgaggt ccttgtccag gttgtgcaag agagagctat cgacgttgta
     5941 gcccgcgatg ataacgcgca taaatcctca gatcagagct tggacactgc tggcaccaat
     6001 ccggttggca cctgcagaga gcattgccaa cgcggtggcg cggtcacgga ttccaccgct
     6061 ggctttcacg cctagcttgg gtcccacgct ttgacgcatc agcttgatat gttccacagt
     6121 ggccccagcg ctgccgaaac cggtggaagt cttcacaaaa tcggccccgg ctttcttggc
     6181 gtagaggcag ctggcgatga tctcatcttc agataaatag caggtttcca ggattacttt
     6241 caagcgcact tcttctttgc ggcaaacttc cgccactgct ttcacgatgt cgtatgagag
     6301 cttgggatgg ccactgcgaa tagcactgag ggattgcacc atgtccactt cctgaactcc
     6361 gtgctggatc actgctttgg cctcttccac caccgcctcg aggcatcctg cccccagggg
     6421 aaagttgatc acggtgcagt tcttgaccgg atttgccaaa cgcagccgaa cgatgtggct
     6481 gaaccatgaa ttcacgcaga ccgaggcgca gtggtgatcg ttggccactt cgcagagcgt
     6541 gttcacctct tcctgactgg cattggcttt caacaccgta gcatcgatta cagcggctat
     6601 gccctgggat ggattccata actggtctgg ctggtcggcg cgacagacgg cacactgcag
     6661 acaaatgacg tgatcgcctc cgcaacggca gagctcatta ttgggaaaca accgtctggc
     6721 aatggcttcg atcttattca tgtcaaactc ctgtcgattc tcttaaaaag tagcttgaga
     6781 gggttgagaa gatttagagg gttttagccg tttatctctc agctttctta accctctcaa
     6841 ccttctcaac ttgtttagtt gatagatggt taagggccag ttgtccgcaa gccccattga
     6901 tatcctgtcc cttgcttttt ctgagcgtga tagcttgcgg caacgaagcg gccgcagcca
     6961 taaagctctc cacttcggct ttggtgggag cgcggtaccc gagctgggga acgggattga
     7021 aggggataaa attgatcttg gaggccaggt cgccggtgaa acgacgcagg gctttgatgg
     7081 ccgtctcatc catatttaca ccgggaatga ggatatactc gaaggttatc cgaaagggat
     7141 ttttgccttt aaaatactgc agggcagtct ttaactgggg aagagggtag aggcggttca
     7201 caggcatcag tttgtcccgg atggagtcga cggctgaatt gagcgaaagg gccagtttga
     7261 ctttaacgcc catgtcggcc agacggataa tgccaggcac gatcccacag gtggaaaccg
     7321 tgatccggcg agggctaaag gccagtccgg cagggtcctg gatgatccgc agggcttcca
     7381 tgacgttgtc aaaattgtct agcggttcgc ccatgcccat aaagacgata ttggtgaggt
     7441 gatcggtccc atccggagct tcggattccg atccgtcgga agcgttctgc tgtatcagta
     7501 tctggctggc aagcagcacc tgctgcacta tttcatgtgg cagcagattt cttcccggcc
     7561 ccaaagttcc cgtggcgcag aaagcgcaag ctctggcaca gcctgtttgg gaagacacac
     7621 agagggtcct ttttttacct tcagggatca gtaccgcttc cacttccagc ccgtccttga
     7681 gcttcaggag cagcttgatg gagccgtcgc gggagatctg tctgtccacg atttccggca
     7741 tgctgagatc gaaggcctcg ttcagccagg ctttgaaggg tttaggcagg ttgctcatgt
     7801 gatcaggatc aaagaccatt ttctgataga cccagctcag cagctgtttc gtgcgaaagg
     7861 ctggaaagtc tttaattatc agggcaccaa gatctgaggg cctgattccg tagaggctgt
     7921 ttagcatggt tccagatccg gaacttccac cacggtaagc tgttggtatt cttccaaggg
     7981 cagctcggtg agcaactggt cttcatcggt caggtggacc cgcttgttga gcacgtcgtt
     8041 tttgaagaca tacatcttct tgcctttcag catcacgcag gtccccagag taggaaactc
     8101 gcgggattct tccacataaa aatcttcttc aaaattgagg cagcagagca gtcttccgca
     8161 gggccccgaa attttggaga gattgccggc cagattttga tctttggcca ttttgatggt
     8221 aacctgattg aaacgcttca gaaagctgcc acagcagtat tgattgccgc acatcccaaa
     8281 gcctcccagg cgtttggcct cgtcgcgccc ggtggtctga tgcagttcga tgcgggtctt
     8341 gaacacgttg gcaagttcgc gcacgaagac tcggaaatcg atgcgtcctt cggcggaaaa
     8401 gaaaaaggta agcttgttgc catcaaactg atagacggtg tcgatcagtt tcatcgtgaa
     8461 gggatagcgc tccaggatat ccataaaggt cttggcggct ttttcctctt cgtagctcag
     8521 gttgcccatc ttctcgatgt cttcatcgga agcccgacgc ttgatgtgat actgacgggg
     8581 gccttgaacg gaggccgcga tctcggtgct gttgatgctg agatgcacca cctgagcgat
     8641 gtcgtctccg cgttccactt ccacaatgat caggtcttcc ggctggatgg gcaggccgga
     8701 gctgttttga taatagccca gccgtccggt gcggaattcc acttctatat aatcttgcat
     8761 ttatactcca ttagaggttt tggaagccgc tagatcctgt ctcgacctgg caatttccag
     8821 atagagctct cgctcggcct tgccaagttg caggttgcgg cgttctttca ccaggtttgc
     8881 cgtccacagg aaagcaggta cccgataaga tttcagatcg tttgccatgc cccaactgag
     8941 gtctgggata aagccatcga tcagggggct gccccacagg tttgctccca cgccgatcac
     9001 cgtgccggta ttgatggagc aattaatgcc cagtttgctg tgatcgccaa tcacgcagcc
     9061 cataaacatg gagccgctgt cgatcttttg accggcttcg tagctgtaaa aatccacatt
     9121 ggcgtaggtg tttttgaggt cgctgttatt ggtgtcggcc ccgagattaa cccattcgcc
     9181 cacaaaggaa tgtcccaaaa atccgtcgtg ctgcttattg gaataggcct gaaagatact
     9241 gtcttccact tcgccgccga ttttgcagac gggcccgatg ctggtgccgg gatagatttt
     9301 ggcaccgatt ttgatctggc tctttttgcc gatatagcaa ggccccatca ggacggcgtt
     9361 gggcatcacc acagctccct catcgatcac gattggtccc ccggaagcat ccaggaccac
     9421 tccgggtttg agggtgacat cctcgccgat ccacacagaa taaggatcga ggaccgtgac
     9481 gccgggttcc gtctcaaagg cgttgtcctt atcatagaaa aactgctcaa aatcttgtct
     9541 tataagctct ggagaggcat gcacgaggtc agccaggtgt ctgaacagca ttcctttccc
     9601 ttcagcctcc tttacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctt
     9721 gtaaacgttg gcgcagcttg aggatgccgc aacgcagatc tcccgtggaa cgggtgaggg
     9781 caagggggta ataagcaatt ggctggtcgt cgaaaatgat cacactcgtg ctcctttggg
     9841 gggtctttta ccgtgccaga tgaagagatt aaactggccc agcaccatcg tgaagaccag
     9901 gaaaaaggtc tggatcacca tccagatggg gtagaagcgc atcatcctgg cagtgactcc
     9961 aaaccgggac cgggcatagc tgagaaaagt gatctcgatc agcatcctga aggcaaagac
    10021 cgccagggcc agctgccagt tgaagatcag catcaagggc ccgccccaat acataaaagc
    10081 catgctgagc agaatgaaaa agaactccgg attgaaggta agctggtatt tcatgttgga
    10141 agcccagcgg gagcgttgat tgaccaaccg cttccaggac ggcaccggat gagtgtaaac
    10201 gaaggactgc ggattgaaat tgaagatgat gcggtgaccg ctgcgacgca tcaactgcat
    10261 gaggttgacg tcgtctccgg agatcagatg ccggatcttg tcaaaaccac ctactgattc
    10321 ccaggcggct ttggtgtagg cgaggttctg acctatacag gcccagcttt tgccaagagt
    10381 gtaaccaccc cccagcacca gataagtggc cgcaaaatca aagtgctcat acttgtgcag
    10441 cagtgatgct gtgccccagt ccctgatctg ggtcctggaa tagcccgcca ccatcgaaac
    10501 gtcatcggta aaggcttcca ccatagaact cacccaggtc agcggaacca cacagtccgc
    10561 gtctgtcgtg aggatgagct ctccacccgc tgctttgatg gcgatttcca tggcctgctt
    10621 tttgggggag accaccttct ctctgccctt caccttgatg taatggaccg ggacgccgct
    10681 gtcttcataa gagcggatga tttgctctgt atcgtctgtg gaatcgtcat cggccacgac
    10741 gatctcatag agctcccggg ggtacttttg attgaccagg caggtcatca gagtgggcag
    10801 attggctgcc tcattgtggc agaccaccac gatcgagaca gtccgctttt tgaagctcag
    10861 gaggggctgg tagttcttgt aaccccagta gaaacggatg cagtggacaa tgtaggtcac
    10921 aaagccactg gcaatcagga ggatcaaaac gcggtaaagg atggagtgga tcaccaggag
    10981 gaccctctgg attcagttga gaagattgag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn gcgtaatccg cgggaaatga gtaatctgcg ggaaaataat ctttctcaac
    11161 ctggcaaagg cctcagcggg tttccagacg gtcgttgtaa ccccagcgcg tagggtagaa
    11221 attgaactgg agggtatcag ccaccgcggg tgggatattg tcttcgataa agacttcctc
    11281 aatcccgccg ccattggtgg taaaaccggt gtagggattg atgctgcgct tgacgatccg
    11341 ctccggaacg ttgaaggcat agcggggatc gtctgctttg gggtaacggc cattgttgtc
    11401 gatcttgata gcctgggcca tgatgcgtcc ccacatgttg gaggcttgag ctttgccggc
    11461 gaagaggttg ctgttgtcaa atccagtcca gataccgagg gtgaagagct cgttgaaacc
    11521 gatataccag gagtcgtagt tgtttgaagt agttcctgtc tttccagctc cgggccagga
    11581 atatccgctt ctggcacctg ctccggtacc ggtcttgaat accgattcca acatgctggt
    11641 catgatgtaa gccacttccg gagaacagac gcggtgcttt tgccccgtcc cgcgttccag
    11701 aatccgtcca ctggtatctt cgaccttggt gatgaagact ggcgacactc tcatgccgtc
    11761 attggggaag gtggtgtatc cggaaattag gtcaatgggg atgatctcat gcgatcccag
    11821 ggccgcggtg aggttttcgg cgcggcgatt caggccaaag cgcttgaagg cctcgttgac
    11881 gacgtcgagc cccagatcat aggcgcattt taccgcccag atgttgtagg aatgagtgag
    11941 ggcgacgcgc atcctggtgt atccgtgata attacgtccg gcattttggg gagaccagtt
    12001 tcccagcgta aagggagaat cgctgattat ggtagagggc gtgtagccct tttcgatcgc
    12061 cagagtgtaa aaagtgggct tgatggatga accgggctgg cgtttggcct gggtcatgcg
    12121 gttgaacttg ctgtgttcaa agtttcttcc accgatcaga gctctcacaa agccggtctt
    12181 gttttccatc aggacgagcc cgccctggat gtattgggta tcgatatcct gcgcgccagc
    12241 aggcacatga gaatatttgt tgcgatggcc ggagctgttt tcgatgttgg ctagatagtt
    12301 gttgagaacg gaatctgcta caccggaaat atcagggtca agcgtagtgt aaatccttag
    12361 cccgccttca aaaaggcgct cggtgccgta ttttttctcc agataagtcc tgatatgctc
    12421 gataaaataa tcggaggcaa atctcctcat agagccggcc tggggcttga cgggagtgtt
    12481 taccgcgatg gcataatctt cctcgctgat cttattggcc ttgaacatcc gttcgaggac
    12541 gtggtccctg cgacgtttag cgttttcggg cttgcggatg gggtcgtagt aattgggcct
    12601 ttggatcatg cccaccagca tggcggattc agcgatgctg aggtcccggg cgtgtttgtt
    12661 gaagtaatac agcgccgcgg tctccactcc gtggatctgg cccccccaga agattttatt
    12721 gaagtagatt tccagaatct cttccttgct gaaggtggct tcgatgcgca gggccagaat
    12781 agcttccttc atcttgcgtg atacctgttt atccagggtg aggaacatgt ttctcgccat
    12841 ctgttgtgtg atggtgctgg ccccctgaga gaaatcccgg gtgcgtatat ccaccagcac
    12901 ggccctgaca aagctaaagg gatcaatgcc aaaatggcga tagaaacgct tatcctctgt
    12961 gactaccaaa gcgtcgatga gatggggagg caattccttg acggatacca gcttacgctt
    13021 ttcaaaagcg aacaggtaaa tcatgcggcc attgcggtcg tagacttcgc tgccggtgcg
    13081 gagcacaaag ttgcgcagct ctgccgtggg aggaagatca tcccggtaaa accagaacaa
    13141 tcccagcacg atgccggtca gaacacagaa tcccaggccc agatagccca gaatcctcat
    13201 gagggtcttg cggtctctat aaatcattat taacaagcct tagttcattt atgcgtggga
    13261 atacagcctg cgcaatccgc gctattagta atccactgat caatcccaaa aaaagcagga
    13321 taggtgtcaa cataaaaact ccccgctggt gcagaaccac ccacttgatg atgagcaact
    13381 gccccaggtt gtgagcggta gcgcctgcca gactgattcc atagatgctg aaaccgagcc
    13441 gggaacgtct ggccagttcc atgatcgcaa tggctgccag gcccgcaccc aaagacaaaa
    13501 tggtaccggg agtcagaaaa gtgaaagttg ccactcctcc gatcagggat tttgttatgt
    13561 ttacagctac tgcttgccat acttgctttt ccatcaccag gtataggacc accacgtttg
    13621 agagccccag cctcaaaaat ggcagtggca gacttctgga caccaggctt tccacaatat
    13681 gaacgctgca ggcaatggcc gtgacgaaag ccagaaagat caggggacgg ggtttaacgg
    13741 agctcatggg aggccaaaga acttgcggat caatggatca ggcaaggagg gcagatcatt
    13801 cagaggccca aaatagcggc tttctccgga ttccagaaag aggaccatgt ctcccaccct
    13861 ttccagatcg cggacttcgt gggtgatcgt gatggtggtg gcggttttgg aacttacgat
    13921 ccggttgata tagtaaagca cctcatcaga agtgatggga tcaaggcctg agaccggttc
    13981 gtcgaaaacg atgtagcggg gattgtaaac cagggccctg gcgataccca cgcgttttcg
    14041 catcccaccg ctaagctcgg acggaaactt ttccatagta tcgcccaaac ccacttcatc
    14101 caaaactttt tggacctttt gccgcacctg gctggtgtcc ttttcgcctc tggtataaag
    14161 cggcaaggcc acgttttgaa acacgctcat ggaatccagc agcgcggcat tttgaaacac
    14221 gatggcaaac tggtcctggg tagattcgtt tccggagtcg ggatcggcgt acacgtccct
    14281 gccgtcgata gtcacgcttc cagcggtggg catgtagaag cccatgaggg tcttgctcag
    14341 cacggttttg ccgctaccgc tgcgtcccag gacgatgaga ttgtgcccgt ccggcagagt
    14401 aaagctgaga tctttcaaaa tgggcttatc acccatttga aggctaagat tctgggcgct
    14461 gatcacggtt cagaaaacct gccgtccagg caaagccgag gccaagagcg ttggccagaa
    14521 agtcatagat ggaaactgag cggcccggga tgaaggtttg atgatactca tccaaaagag
    14581 cgctgcagag caaggccagg tagatcagag aacgcttgga agccttgatt tgccttgtct
    14641 tcaaccagga attcaccaag aggccgagga caaagtaaac tccgatatgg gcaagtttgt
    14701 cgattgagag gatctgtaag gaaggcagat cgtcagaggg gacagaggac agtatccaga
    14761 tcagggccca ccacaggagg gtgaggacca aagaggtggt gccgggcttt ttaacgctca
    14821 tggggtgatg tctttcagct cccaactgct tccgatggca ccttttttca tgctggcttt
    14881 taccaccagt cctttgccgt ccacccagag ctctaccttg ctgccttttt gaaagaggtt
    14941 gtgggttacc atatccacgt agggagcttt gctgtgagtg gcgctgctga ggctaagctt
    15001 gtagcccctg gagctaaatt tcccggcggc ggttttaatt accttgttgc ccagataact
    15061 gacgctcact ttccagagcg aaccgttggc gtcaagctca tagtttcccg cacccgcctt
    15121 gccgtcacta agcatggcga taaaagagaa aaagtccctg gtagcgggat ttatcttgta
    15181 tgtgatggtt tcaccgttac gggagtggga ttccgcggcc aggctcttgg agtggtcata
    15241 gaccgtgagc acgttgtcac tcagcttgtt ctgacgcaca gcacgattgt acttcagagg
    15301 gagatagccg ctactgtatc tgatcgtgta ttcattgtct atggagggaa agagggcatt
    15361 ggtgatcaga gatttggtct ttacactcac aaggctggca cctggatcac gttcgatgat
    15421 gaggttggcg acgctaagcc ccatggcttt gacggtgtag ctcatgcgct tgccctgcaa
    15481 gctcaggcag aggaccagca tcaggatggc cggcagccag tgcttagata tctgtatctt
    15541 catttagagt aattacttcc acctgcttga tgctcttgcc gtcactgtcc agaatagtga
    15601 gttttacccg gccgtccaga gtgtggcttt caccggactg gggcacgtgg ttgaagtgag
    15661 aaagcaggta ttcggccagg ttgtcaaact cctccggatc tatatctgtg gagaactggt
    15721 ggttgaactg acggatgttg aagctaccgc tcacggtaaa atgattgtct gaatgggccg
    15781 tgatctcagg ggcttcttca tggtcatatt catcatggat ttcccccacg atctcttcca
    15841 ggatgtcttc caaagagatg atcccactgg tgccgccata ctcatccacc actacggcca
    15901 actgcatctt gcgggtcttg aactgattca acagggcctg gaccttcatg ttttccgtca
    15961 caaaccaggc tggccgcatg atctcggtaa tggaagtctt ttccggatag agcagcatgt
    16021 ctttcacata gacgatcccg ataatgtcat cgatggtttc ccgatagacc gggatgcggg
    16081 aataacccgt ctccaaaatc aaatctctca gctcttccag gctggcatct tcgtcgatgg
    16141 cactgatctt cacgcgtggt acgtaaatct cgctgatctg agcttcccga aagcggaaaa
    16201 ggcccaccag catttgcttc tcatggtctt ccagcgagtg attggagctt tcggaatgga
    16261 tgagattgtg aaattcctcg ctggtaatgc tttgtcctgg ataatgatct gccccctgct
    16321 tcctggagac cagcattgag aggcgctcaa acacccacac cagcgggaat agcagatatt
    16381 gaagtatctg tagcggcatg ctggtagtcc gggcaaaagc actggctgta gacaatgcca
    16441 gcagttttgg gatgatctcg ccaaacatca ggatgacgac tgtggtgagg atgatctgca
    16501 ccgtcaccag cgaagagagg ctccaactgc tgtatttctc cgcaatcccg atcaccgtca
    16561 gagtggccag agacgagatc gccatattga caaaggtgtt tcccagcagg agcgtaatca
    16621 gcagccggcg gggtttcttc aacagcgtca aagcgcgctt cgctcccgga gtaccaaatc
    16681 cttccatctt ttttagatag aggcgtgaga gtgagaaaaa agcggtttcg gatcctgaaa
    16741 agaaggctga caaaaacagc agcccgatca atgtaaacgc ggggccttcg tccactttag
    16801 catgcaccta tgagcttgta gcgaaagtat gtttctttgt gttccatttt ttctttatct
    16861 tgggttttaa tgtggtcatg gcccagcaga tgcaaaaggc cgtgaatcaa taaatactgc
    16921 agctcggttt ccaagctgtg aaaacccttt tgtctgtcgg cctgatttat gtcaacaaca
    16981 atatcacccc ttaagcactt caggcctctg acagcctgct ctgaagggat cgaatcgctc
    17041 aggaaactga ggacgtcggt atcctcattc ttaccccggt aggtcttgtt gagctggcgc
    17101 atccgtttac tgtcacagat cagcaaggta agctccgcta cctgatcagg aatatcttcc
    17161 gcgcagatta gctcagcgat cctgattagc ggcaggggat cgatctccac accgctttcg
    17221 ttgatgactt caagcattgg caggagcttc cggtttggga ttttcatcag gatagtccag
    17281 acgcttgcga tagatacttt ccaacaccgg gagcatagtc tgcttagcct taaagaggtc
    17341 tttgatggtg atgggggctt cgtcaaactg accttcccag atgaggcgct ggaccgtggt
    17401 gtcgatgatt ttttccagct ctgctctgga actgatgttc ttggctttgg tagtggattc
    17461 cacgatatct gccagcatca ccagagcggc ttccttgctg agaggtttgg ggccgggata
    17521 gctgaaatct tcaggattgg gatcggagcc ccggcgttcc gccaggtcca ggaaatagcg
    17581 gatcttgcta ctgccgtgat gctctttgat gatgtcgatt acatccgggg ggatgtgata
    17641 tctggacgcc aggatgatcc cctctttcac atggtctctg atcattctgg cgctttcctg
    17701 tgggtccagc agatcatgga tctctgagga cgcggcgttg ttttcagtga aaatctcgtg
    17761 gttgatcacc ttgccgatgt catggtaata gctgcctact ctggccagca agggattagc
    17821 gccgatggcc tcggcggccc gttctgccag atttccaact accagactgt gatgataggt
    17881 ccccaccgcc tgagtggcca gcttcttgag aaggggatga ttgaaatcaa gcagttccag
    17941 cagtgcttgt ttggtagagc gattccagcg ccgttcaaag aaagagatca cagcagcggc
    18001 accgagcacg gaaaggatgg aagagattaa agcatatccg gaattcctga ggatcgtgag
    18061 caggggatcg ctcttgtaga gtgccagggc cagattgacc gcaagggtgg aggcaaagag
    18121 cgcggcccac attctgatgt attcatgcca ggcccccagc ctgcggatca gcaccagggc
    18181 tatcagagtg gataacagca acagcaccgg ggtgtaggat tcccagttca caaagggatt
    18241 gaggaccatg gcgttgcaga tggtcagaaa tatcccaaat tcaaagccca gcagtgtggc
    18301 cacgctgagc gtgataagcg cgaaaggaat gaggatgttg ttgagaccga ggatttgatg
    18361 gttgatgaca gccaggacgg ccagaaagac gaaactcagg ttgatgggaa actgagagac
    18421 ctcctgggcg gagtccttcg gcaccagggt aggcaaggcg tagcttgcga ttaccaggat
    18481 catcagcata tagagcaaca tccccagtga caagcccatc tgttcccagg gtgaacgtcg
    18541 cacgtctctg gatttgtaag cctccgtgag cgatttgagc ttgttgatat ccgtttccgt
    18601 taccctggaa ttcttgcgga tgatgatctc gttttgcagc acctcgccct ctgaggctgg
    18661 tatcgcactg acgctaagct gcttaagttc ctcaaatttg gcctgattga ccaccaggtc
    18721 cgcttcgatc agaaacttgg aaagccagag ggcgaatcca ccatagccac gttccgccag
    18781 cctgcttcga aacgcttccc gggcggcttc aaggtcttga aaactatccc ggttggcgcg
    18841 cctgagctgt tcatcgctga gcactgtgat gctgtcactg aggatatttt catagacggg
    18901 ctttttatac accgacgaga tcgactcgta gatatattca taagcccttt ctcgagaatc
    18961 aggattgagt gccaaagcag taaaagcggg gtccggatca gcgcccagcc cattgagagc
    19021 ggccctgagc tgttctctct gagaatcttc cggatactcg gacagcaagg taaagagctg
    19081 atcgagtttg ctaaatgagt cgaatctgac ctgttcggaa acgctaaacg gagcttcaag
    19141 ctgctccaag gccagatcgc gatccagctt caactgcttc tcggatttga gcacggggaa
    19201 gtcgaagggc gcgatcaatt caaaatcggc cacctgtcct accgtgagac ggtattccgg
    19261 atacgaaaac cgtccgacgg caaagacgtg atagagcgcg gccagagcca acgccgtgat
    19321 caggctgaaa ataaaagctt tggaactcat gggagcatct ttgggccggg ggtcagattg
    19381 tcaagcccga tctgtgattc tcactcttta aacagcgcgt aaacagcttt gatgtctttg
    19441 atctttatca ctcccttgcg agctttcact ttgctgtgag cggagacata tacctgctcg
    19501 tagcctagtt tgacagcttc attgacgcgg ctgtccgttt gcgaaactgg cctcacttct
    19561 ccattgaggc ccacttctcc gataaaaacc gccttttcag gaaggggagt atccttgagg
    19621 ctggaaatga tcgctgccag gatcgccagg tccaaaccag tatccccgct gcgcatccca
    19681 cctgccagat tcacaaagac atcgctgctg cgcagataaa gggccagatt cttttccagg
    19741 atggccagca gcatggccag cttcttttgc tccagaccga ctgccacccg ttggggtgtg
    19801 ccgtaattgg atcctgtagc cagggtctgg acttccacga tgaagctgcg ggtgccttcc
    19861 atgacgcagc ctatcgaagt tccgctcaag gctggatccc gggagaggaa aatgtgattg
    19921 ggatcggaga cttctctgag gccggtgttg gtcatttcga agatcccgat ctcgttggtg
    19981 gagccaaagc gattcttgac ggcccggagg atcttgtatt gccccctggc ttcaccttca
    20041 aaataaagga cggtatccac catatgctct atgatcttgg gacccgccac caggccttcc
    20101 ttggtgacgt ggcctaccat aaaaatcggc aggccaagag tcttaccggt ccgggccaga
    20161 cggttgcagc tttcgcgcag ttgcgtgaca ctgcccggaa tcgaatccag ggagggaata
    20221 cttaccgact ggatggaatc gaccaccaca aagtctggac tggtctgatc cacggtctcg
    20281 atgatggctt ccacgtcgtt ggtgcaatag agccagatgt ttgggctgtt cacatccaga
    20341 cgcgacgccc tgaggcggat ctgctgatcg ctctcctcgc cagacacata cagcaccttt
    20401 ttgtccatcg cgcccagcca ttgcgaaagc tgcagcatca gggtggattt gccaatacct
    20461 ggctcgcctc cgatcagagt gagcatacca gatacgatgc ctcctcccag caccaaatcc
    20521 aactcgttga taccagtagc aattctggag atagcgtccg tcttgatgtc tatgattcgc
    20581 tcaggactgc ctttttctct ggccggtctg gttgcgccgc cttttttccc gctcactcca
    20641 ctggattctt tcatggtacc ccagcttccg caggcggggc atttaccgct ccatttactg
    20701 gtctcgtgac cacaatcgga acaaaaaaaa ctctgggcca tcaatcctcc ctttgagtgc
    20761 cataattacg gaatcaaaaa aaatggcaag cactattttt atctcctaat aacctagtag
    20821 ctttattttg agcacctcac caaaaccttg ttcagtcaat tcctaaaaaa agtttcttgt
    20881 attttgtggc agtccggatt attattgata tgaggtaatc ttcatgaaac ccaatatcgt
    20941 gatatttggc actccgacat gtgcgtggtg caaaaaagtt aaagaatata ttaagtcacg
    21001 tggattcacg tttaagtacg tggatgtctc gactgacggg tcagctctgc gtgacatggt
    21061 gcgcaaaacg gggcagcaag gggtcccgca gctatggata gacaacgtcg ctgtagtcgg
    21121 cttcgatcgt gccaagatcg ataatctgct ttcaaaaaca aacaaataaa actaggagaa
    21181 caaaaatgag cacaagcgct gaaaatttcc acaatctgat cacccgtctg caggtggttc
    21241 tgagtgagat cgactacgcc caaaaggcct gcctgcaagc agggaagatg gagtgtcagc
    21301 ttctcaatca cctctatctg gtcaaaaccc ctgtcaacat gaacgaactg gccaaggtgc
    21361 tgggtgtttc gcacagtcgc gttacccgta ttatggacaa cctggtcagc aaaaaactcg
    21421 taacccgccg gccctctgaa gaagaccgcc gctgctggtt tgccattatt acggataagg
    21481 gcagaaagct tgccgaaaac agccagcaaa ccgtcgtgga ccagcaaaag aaaatcatag
    21541 cccagctgcc agccaaggaa gtggacaaca tctttaaagc cctcaaagct tatgttgaac
    21601 agtacgaggc ggtgctcaag gctacctacg tggagctgta atgaccacta aagccagtat
    21661 gaaatgggtc aaagacatgc tctttgacgc tgagataacg ggacaccaca tcctcatgga
    21721 tgccgatacc cagtggggcg gcaaagacca gggagccagg cctaaaccct tgcttttggc
    21781 tgctttaagt ggctgctcag gcatggacgt ggtctccatt ctggataaga tgaaggtccc
    21841 tgaatataag ttcgatgtcg atatggaagc tgattccacc acagaacatc ccgtcactta
    21901 tcacaccatc cgcatgaagt ttctctttga gggccaggac ctccccaagg ataaatttat
    21961 caaagccgta aagctctcca ctgaaagata ctgtggagtg atggcgatgc tgaaaaaagc
    22021 ggccaacatc atcgtgaacg tctatatcaa tggtgaggaa gtaaactaat gaaacacgta
    22081 ttcctattga tcctgatcat gagcctcagc ctgacaggat gtaaagcgaa agacacccag
    22141 gagctcaatc ctgaagcgca gaacaacccc gaagctcaga atgctcccga actggggaat
    22201 gttttagatc ccaatgtggc agccgataac acagacagcc ccacctggct tgtcagtttc
    22261 gaccaggccc tcgctcaggc caaagcacgc aatctgcccg tcctgatcaa tttctcaggt
    22321 tcagactggt gcagctggtg catcaagctg gcgaatgagg tcttgaacaa accagaattc
    22381 ctgagctacg cgaaagacaa tctggtgctg ctcaatctgg attttcccag aaagacccag
    22441 ctgcctgagt cgcaggccaa ggctaatgaa gcaattgccc aacgctttaa gatcacagga
    22501 tttcccactg tcatcctgtt agacagccag ggagagaaga tcggccagac tggttatcag
    22561 ccaggcggac cccagaaata tatcgaacat ctcaagcaga tcatgaatcc aagcaagtaa
    22621 ctccatctac catacaatcc gcaaaaccgg cacacagacc atgtgccggt tttgcttttc
    22681 gggaaaatct gggcggtcaa tcctgaatac cctaaagact gctgaataat tggtatcact
    22741 ggctgaagag cttgggcaaa aactcccgaa accagaggct gacattaccc cgttggagtt
    22801 caagcggcca cgataaagct atacatggca acgagtttgc gggccgcttg gacttcaacc
    22861 ag
//
LOCUS       DAOSFN010000018        22566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1836_length_22566_cov_9.022123, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000018 DAOSFN010000000
VERSION     DAOSFN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 22566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22566
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1836_length_22566_cov_9.022123"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            580..1134
                     /gene="efp"
                     /locus_tag="PLO35_02395"
     CDS             580..1134
                     /gene="efp"
                     /locus_tag="PLO35_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020806954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOH78542.1"
                     /translation="MADIRNGMIIEFKDGLFEVVEFLHVKPGKGPAFMRTKLKNVRTG
                     RVLENTFRDSDTFNEVRIERNKMEYLYKDGTFFILMDLETYEQIHVDEAVIGEREKLM
                     MDNMEVMVMRAPDGEILGVELPLTVIQTIAECEPNVKGNTASGSGKTAYTETGLRITV
                     PFFVESGEKVKIDTRSGEYVERAN"
     gene            1175..1381
                     /locus_tag="PLO35_02400"
     CDS             1175..1381
                     /locus_tag="PLO35_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78543.1"
                     /translation="MGKVKSFSAKLAHETSNEGKVMCPVCNTEIKKVKVVGNKKGQAG
                     WSPRYDFVKVCKCNEADFLAGKIL"
     gene            1576..4674
                     /locus_tag="PLO35_02405"
     CDS             1576..4674
                     /locus_tag="PLO35_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78544.1"
                     /translation="MLPIKPYLVLSLMICVASTLLALVPPHPMSTHVPSSWEPVRIEA
                     PLSQDGSLLKAQRVLPNNILVLRVQFSDVGFVSQPAYPDQLAHDDVFFNRWMLHLSDF
                     FADASHYQYELSYTLHPEVITLPHPMAYYGGDTSEKIDARIAELARDLVDAVDDEVDF
                     NQHGGLIIFHAGAGQESDINGIRTDLIWSTFLTRKTLQAAFAPNNDSYPGLSTDDGAN
                     LINIVIVPEHEFQDYFPAEGQQDAEAYLFSIYGVLTHQFAHIIGLPTLFDNDSSNGTS
                     QGIGNWGLMGTGVWNASGYVPAQLSAWSRMFLGWETPISVSGDATGLLVDYFLDHATG
                     RNRLYKLPISATEYFLIENRQQNPDGSLDPYSNTPSYTFKLLPEGEQDYYENYPLLPF
                     FNFMENRYIGSEWDFFLPGLGGPVPSGQMMLQDGSGLLIWHIDEAVIAENFTTNFDRN
                     RINADSRHKGVDLEEADGIQHLDTAIYDPYKWGSPFDAFRQDNNEYFGNPTYQGLMSL
                     PTAESYYGGIPLEVYDIGPNGNQMSFSVRFSWRLSSDYVGPNPINAAILDLDGDQEDE
                     VFYAMPDGELYAWDDEQMMPGFPQRRMPVTQPYVWDEDHLYLPMELGPLARLYRMNST
                     GGQYVFTQSGASWASHPVDIGNQLMLPLNVDGGAELRRYDKGTTSTPLGLSIDQPIAS
                     NLVWFRNQLNFLTAEPGLYYKLWTYDPATGSSSSLSLPIPADSTIVGIFKAPLIKGSV
                     NGELLIQTAKAVYVFGSDSQGLPVFVFSHDSVCTAPLTLADLDVNGSLDIILGTENGV
                     IVLDYSGQRMSPENLEGLSSEEGGFSAGALALDIDMDGKPELLGNFANNQIKAWSASF
                     QLLTGFPDSFAERSRNLPLVSLASDGNRYIWSAADNGSIFRKAIPGPLPNDFNNIWHC
                     EFGNLKRSASIDPDALPNSFESASLFVPNELYIFPNPLKSIYADQLTLNVMTNRDTPL
                     EVQIFDIGGKVIFEQKSIARAYLRNREAIRIPVEKLASGVYITVVKAGKETKRLKFAV
                     EN"
     gene            4689..5594
                     /locus_tag="PLO35_02410"
     CDS             4689..5594
                     /locus_tag="PLO35_02410"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOH78545.1"
                     /translation="MNMNRYILTFLLLSATLLGADLHPEAGTYGFQFLNISSDPVSMA
                     LGGRGIHATMDRAAFIRQPAVGTVNSHRSLGASHSPWLDDTTFNQLYYGYSDRLSHFG
                     VALRNLDYGELESRDETGTLIGYYHPSDVSLMANYAWRLGPSTYVGMNGGVVYQKLST
                     ASSYGINVDLGATILPPFPNSLLSVSVRNVGDSSKMNEVPTRMPVSLEADLSKQWQIE
                     QGSLIVELSAIKAVDAHLKGVVSTQLTVLDRIALRGAYKLNYDAEGLSAGLGVKVGKL
                     DINYGWADFNSALDDVHSFGLSWNF"
     gene            5603..9778
                     /locus_tag="PLO35_02415"
     CDS             5603..9778
                     /locus_tag="PLO35_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOH78546.1"
                     /translation="MSRSFIILILLGALLGLNAVDFTPNQLIFKTAQPLLEIRSGRTG
                     LSGFDSYLNSLGAYSLRPVTGMPGDRYFLVDLTTAPDWQNLRSGNLSFPGIAYVQPNY
                     LSKMHIEPNDPRYPEQFHYVTSTPQAWNFTTGSELVLVGVVDSGVLIDHPDLAANIYV
                     NPHEIPDNGIDDDGNGYIDDWCGWDFSDAPEMFDVGIGDYVGQDNDPEDENFHGTHVA
                     GIIGAVGNNGIGVAGVAWNVKILPIRAGFRTTQAEGYLQDDDAAAALIYAVDMGCQVI
                     NMSWGDPNYSPIIGDACQYAYDRGVVLVASAGNNPTPNLSYPAKLSTTLSVGAVNRNR
                     NLAGFSSFGPDLDIVAPGEAVLSTYKLTAGEQYFEQNGTSMSAPFVAGAVALLLSLQP
                     GLSPDEVRTRLLTSTDDLGTPGFDMYYGHGLLNVRKLLESISPPLVSITSPIEQQGVN
                     GSFDIIGTIQAEDFFRYSVMFAIKDVPATILDWLDVETHTNMPSYKTQPVSGGVLASF
                     YVPELLPERNYLIRVQYEDRSGKKYNSYVSVQYDSSPPLLRTESLQGHSRYDGQDLRY
                     YLTAAFNEKVRTELRITASDGSVSYRYGTMADSIQIWALPRTLPEGNIDIQIKATNVC
                     NLSALYGDYEDVLNISYDIIPNHGYLPTMIGKARVPLPRTFDFNGDGAMEYVAMDLPN
                     SGYGNVSVYQPEAGGHVPKHDFMDSFWLLDTGNTNTQGQELLYIKGDTCFLEETQETH
                     QYPNLVIWEEASISGGVIADYSGDNRSDLLLVKNLPTEQVVQAYKRGTGATITAKNTL
                     RNITPTNVRNTFVPTIIVERLDDDNYPDILTADTDGDIMIFEIMNDNMDAMVWHHRMP
                     VGNTYTVTSGDYDGNGKQDFFIGGYYTDILNPDMNFWYFEGFKNVANDQYSSMGSLLF
                     NNVMSQNAIQSFDLDNDAKDEIILGISPNLYIVKYQEGKFQPVFQGSSMRSYQIMAWK
                     DANNRPYFTTNYAVGDSTQFAQWTSEDPYTGPPTPANLHTVPIGSSSARISWNDSGAS
                     YYRLYRMNEAGETEMFDNLTQTLYLDGGLTEGQSYLYAVTGVDPAYNPSESMPGQWHA
                     VTPMKAPELVEMQMVSASEIKLIYDQILAPTSLNPGLYELDHGMGNPITINSIASQTG
                     VLLRFRQALPEIDGLFSLTLHQITGTTGVAVSQNLLQFPFAPDTEAPQVVSAEVIQGN
                     SSVRILFSEPIGAAPDPSYLGNYRLHAPANDQENRISSVQHQNDSVVLSFLTPLKYSN
                     QAYQVFIENIQDLAGNTISPQNRVARIKLSDVNDLKKLKVYPNPVKGSEHKWVSIHNF
                     PFEKSGRISVYTSSGDLVYSSGLGPFQAVLNNNIWRWDLRNDHGRMVSSGIYFYVVEM
                     DGEISRGKIAIIQ"
     gene            complement(9900..10547)
                     /gene="nth"
                     /locus_tag="PLO35_02420"
     CDS             complement(9900..10547)
                     /gene="nth"
                     /locus_tag="PLO35_02420"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOH78547.1"
                     /translation="MSNKFDIDAVMTALAKHFVEVKTPVVDLIQIQTKDPFKVLVATI
                     LSARTKDETTSRAATALFAVVEKHHDLNKLGLEELDRLIWQVGFHREKSKHLKQLPGV
                     LDDLFDGKVPSEIDDLLKLPGVGRKTANLVRAVAFGLPAICVDIHVHRISNRWGYIST
                     QSPLESEMALRAKLPDRYWLQYNSHVVAFGQNLCTPRKPKCSSCPVYEYCAKIGV"
     gene            10635..11357
                     /locus_tag="PLO35_02425"
     CDS             10635..11357
                     /locus_tag="PLO35_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOH78548.1"
                     /translation="MCGRFAQVIKHDQLQKLERELRLDHKSDQLQANFNVAPTQTVGA
                     FVSQDSGRYLGFFRWGLIPSWSRELPKFELINARSESLMEKASFRGGLQRRRCLIPAT
                     GFYEWRKADRQPFFIQAVSGDPLLMAGLCDAWNGPDGSYLPSLAIITMDANAHMQSLH
                     HRMPVMFNSEQALDWLDHKNQDPAQMNKLILEASQQALDIYPVSKLVNSSRASGPECL
                     EPSGDSAPDEQDPSLFDLLDEI"
     gene            11354..12451
                     /locus_tag="PLO35_02430"
     CDS             11354..12451
                     /locus_tag="PLO35_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH78549.1"
                     /translation="MILDSQASVLVIDDDPHLLELIREQLSESGEYLVVALSDPKEGL
                     AWLMDNQVEVVISDIHMPGMSGIDLIDHIKLYNSSIQVILITGYPDNAYMRSAIKAGV
                     FDFLRKPFPITELLVSVKQAREKYLLLTQNLKYQTELELMVVERTRQLHEANLKLESH
                     YINTLRAMVNAIEAKDVYTKGHSERVTALSMVLGKSMGLSEEELRILRLGSLLHDLGK
                     IGVYKTVLDKATSLSDAEYELVRHHPLIGARIIEPIGLPKEVGMVILQHHEWYNGTGY
                     PFGMVGPEIHPLAKIVAITDAFDAMTSKRPYRPQLDYEQAAKEIERQLEVQFDPNIGQ
                     TFCSNLQELTAILSNPNGLSDYVFGADTQPA"
     gene            complement(12634..13095)
                     /gene="dtd"
                     /locus_tag="PLO35_02435"
     CDS             complement(12634..13095)
                     /gene="dtd"
                     /locus_tag="PLO35_02435"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOH78550.1"
                     /translation="MRITLQRVKYARVSVEGSVVGSIPQGLLVFVGFGREDDSSVLDV
                     AIRKILELRIFYDQDGKLNLSLLDIGGSILAISQFTLYANCSRGRRPDFTQAAPPSLA
                     RELYESFVAKLRETGVPVQTGIFAADMQVELLNDGPVTINLEIAPQATPAP"
     gene            complement(13095..13697)
                     /locus_tag="PLO35_02440"
     CDS             complement(13095..13697)
                     /locus_tag="PLO35_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78551.1"
                     /translation="MTKYLLALMALLMLILSSCFVSSSLSYTESQTQNVPNLVFNPGF
                     NPYSLDPRTALKGWAVDYDPPNSPDGKVLIDTEVALEGKTSLRVDASENAVILISEPF
                     KVIRYGGYYIRAGIRGTSKDMPEITLRFITFKENGKIYNSFKQKILPNEAWNKVSISA
                     GFIRPGVNAGRVAIMIPQFKDGSIWIDDVGCWKVHHFRID"
     gene            complement(13694..14911)
                     /locus_tag="PLO35_02445"
     CDS             complement(13694..14911)
                     /locus_tag="PLO35_02445"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOH78552.1"
                     /translation="MFNRKKFDWNLAVLLLILICMGCVAIFTASTTTIGQHSSTENHW
                     WKQIIFAGIAIGAILLLLKLPMPIFDIVVLPLFLANLLALVLVLFTPAVNGSHRWFSF
                     LGLNYQPSESAKLFTILMVARIISKDHLNELKQVFYGVGLVLVPVLLIIIEPDFGTTL
                     VFMFALLSMLVAAEVPLFYILLMVSPVVSIVSSLSWVAVVIWLALLVFLLLRVRLSWV
                     AITIAGIVNVFIALIMPVFWNGLKDYQQNRILTFMDPMRDPLGAGYQIIQSKIAVGSG
                     AIIGKGWLQGTQKNMNFLPEHHTDFIFSVIGEEFGFVGSTLLLFVFTLFFLRLINAMG
                     EIKVKERKIASAGILAYLMFQTFINIGMNIGVVPATGIPLPFISYGGSNLLINALAVG
                     VVLKYLNERGFMK"
     gene            complement(14911..16812)
                     /gene="mrdA"
                     /locus_tag="PLO35_02450"
     CDS             complement(14911..16812)
                     /gene="mrdA"
                     /locus_tag="PLO35_02450"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOH78553.1"
                     /translation="MNRSIPTLVSRIALGVLFLFLLGALFRLQVIKGDEYRRIAESNF
                     VRIRRVSATRGEIYDSKYRPVAINIPSHNLYLISGKITNIKALGLFLNVYFGINQEEL
                     HDLVFQQRFKTYEEILLVDNIPYETVLALSERMNYYPELVFRNGTTRQYLYPNHFSGY
                     VGRINEAEFKRYRDEDYSLNGYIGKNGLEKYYEVLLRGTDGREILQVDARGKSLDLFR
                     VDGSRDPLNGLSLVLSIDNDLQQFASDNFPVGARGAIVVSDIKTGGILAYVSKPDYDP
                     NVFMQKISPELWSQLNRPEKPLLDRVCQSAYPPGSVFKPVTASEGLETGIINRNTLLA
                     SCAGGLKIGNRFYKCWSSGGHGRTSIVDALKVSCDVFFYDLISRIKLDDAWRHAKACG
                     VIDRTGIDLPSERNGLYPNTQWYQKRLGRSTGLQGYKANLSIGQGEVLTTPLQINTLY
                     AAIARGGYWIQPHLLVKTLGKGRLDREQVQPIVKRRLPWSANTVKCIQDGLWAVCNAP
                     GGTGRALNVAGATAYGKTGSAENALGKVTHAWFCGYIVTDKPEIVVTVFLENAGGGGA
                     VAGPICNRIMNYYVGNLESFKKPAPVPAQFRSLAPVDREEVTEPETPAATESPAPEPG
                     QHMETQSED"
     gene            complement(16805..17299)
                     /gene="mreD"
                     /locus_tag="PLO35_02455"
     CDS             complement(16805..17299)
                     /gene="mreD"
                     /locus_tag="PLO35_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOH78554.1"
                     /translation="MIWKFLWTYLTGIFFLYVQLLVMPAFELAGTIPNILVPWMIWLI
                     WSRPRDMALGVGFIVGAMYDVTQPSLFGLSPLTFLLLGMALSEFRKPFEAESKVARML
                     TLLLANLIYHLVMWLVLGVGHGFTAQLATLNLIAFAYNLVISFVVFWSLQLLSRMRVV
                     IVSE"
     gene            complement(17296..18093)
                     /gene="mreC"
                     /locus_tag="PLO35_02460"
     CDS             complement(17296..18093)
                     /gene="mreC"
                     /locus_tag="PLO35_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOH78555.1"
                     /translation="MIKKYLIPLVLLLFALVMMIGTEEARIARADLLGRTVFYPFARS
                     ISAIRANSALKEEVAGLRVQLADQTLRNLALENELKMFSSGTAVAFDTGLMDFEIAEV
                     IGYAGQFQQRNLIVNKGLLNGLRPESPVVSASGIVGKVINAADTYCVVLPFSNPQFQI
                     PVMDRNTSVQGILQADLRGVISMNMIKLGSQISVGDTIVTSNLSRLFPKGYPVGSVSR
                     IKESSDNLFISAELNPFTQVENLEHVFILRKQMGTLATPQGQGARQQ"
     gene            complement(18170..19207)
                     /locus_tag="PLO35_02465"
     CDS             complement(18170..19207)
                     /locus_tag="PLO35_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOH78556.1"
                     /translation="MSLFDFFGLMANDIAIDLGTANTLVYKKSSGIVIDEPSVVAVST
                     DNKKIIAIGQQAKVMLGKNPDEVKVIKPLKDGVIADFQVTELMLRNLILRAQKKRLLV
                     RPRVIVCVPSGITEVEKRAVRDSALHAGAREVFLISEPVAAAIGADLPIEQACGNMVM
                     DIGGGTSEIAVISLSHIVVHNSIRVGGDKMDTDIIGYLRKKNNLHVGLQTAERIKMTV
                     GSAYPLKQELTMDVRGRDIVSGYPVTIKISSEEVREALSETVSTMIDAIKRLFERTAP
                     ELSADIAERGIFLTGGGALLKGLDEKICKTVDLPVHVVPDPLECVVKGAGKVLDDVDR
                     YREVLIKRIED"
     gene            complement(19352..20722)
                     /locus_tag="PLO35_02470"
     CDS             complement(19352..20722)
                     /locus_tag="PLO35_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOH78557.1"
                     /translation="MKELGRTAVIGRTFERPWLQACLFLLFSLLFWSDLPSCVMSAMI
                     SARPLKSFPRSGFPFEYTAYNDPWDYFGFVMANSQPYGNSDGYGTVAYERGRLFLSQS
                     DRWYKRVLQSTDFGNTYYTGRYLDRDYSSQFWELDVLDRAMYSILGTNSQSHIAMIHG
                     RNASALSYGNHPFVFDLRDRSLAFMHNGNCYSARNFMIQRVREINPDENWFVNHPSDF
                     FGLSDPFQWVDSELLFHFIINHIRLNNDDILAGLGAALWELMPWIDYEGAPSFNFIMA
                     DGEKLYAFRNTPLTGLNSHYKLSYKGSENSFWAIRTLYPQPGDASLNKLELVVFEGGR
                     TPVHYPGLEALKPDPIDPEGEITQPGKAMAAFTPGLRLYPNPLRTKSPSLRLSVWIPE
                     LEGRASGSVNIYNMRAQLVYSRTLSTTDGKALLGLELPRLPVGLYLCSVKIGGQVRMA
                     KFSIMD"
     gene            complement(20787..>22566)
                     /locus_tag="PLO35_02475"
     CDS             complement(20787..>22566)
                     /locus_tag="PLO35_02475"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78558.1"
                     /translation="RDFRFAKLIASSASKSIRHHRLTLEYTSNPSWYAVQALPYLMEY
                     PHECNEQIFSRLYANSLASHIANSDPAIKRVFESWGETPASTTLLSNLQKNEELKAVI
                     LQETPWVMDAKDETIARQRLGLLFELNNLKAQEATALAQLQKNQSGSGAWSWFAGMPD
                     SWWVTQYIVEGFGHLDRLGVKSVRNDNGLWTMLSKAVGYIDGQLNESYQWLKAHGLLD
                     QDNLGYMAVHYLYARSFFPDLPIPSYAQEAVSYYWSQAEQYWLQRDFYSQGMLCLALY
                     RKDKLQTANDIIASLREHSLSDEEMGMWWKANSGWYWYQAPIERQAILIEAFSEVAKD
                     TLTVNEMRTWLLKNKQTNNWKTTKATAQACYALLLEGTAWLGSSQLAEITVGGGKLDP
                     SPKNGSWPEAGTGYIKTSWTGSDIKPAMGEVCVLNPNPGPSWGALYWQYFEDLDKITP
                     AETPLKLTKQLFIERTTDHGLVLDPIRDNNRIKVGDKVVVRIELRVDREMEYLHMKDL
                     RASGFEPLNVLSRYKWQDGLGYYEATGDAATNFFIERLPKGTYVFEYGMRAFQKGDFS
                     NGITSIQCMYAPEFTAHSEGIRIKIE"
BASE COUNT         5382 a         6129 c         5927 g         5128 t
ORIGIN      
        1 taaagctata catggcagcg agtttgcggg ccgcttggac ttcaaccagc acaccgaagt
       61 gagaatcgca cctccgtgtc ctgtacgtaa gtccaaagcc ccgtcaggca tagagataaa
      121 cgcataatcc atttaggggc tttgcactcc aagtgaccct tgcgtctgtt tgatccatgc
      181 cattgtattg gcccttaact gtttacctat aactcgcgtt tagcatctcc aacccaaacc
      241 caacaaagtc cgagctgggt cgcttttgcc ttgcctttgc cgcagctata cgggtaaggg
      301 cctaagcgcg tgcataacat atagatatca ataatctcca cccatccgct tatccttgcc
      361 ctatcacccg cgctggagat accgtgccct ctcccgcgat tttcggcgga gggcgcccgg
      421 catctctacg gcaacaaaca ggcaagcaac agcgtagctc tcacagcgtt gctctcacag
      481 cgcagctctc tccccccaaa atttcccttg ccaaaattcc cagcttgaaa agcgtggttc
      541 attcagtcta aaactttgag gttaatactt atggcttcca tggcagatat aagaaatgga
      601 atgatcatcg agttcaaaga cggtctcttt gaagtggtgg aattcctgca cgtcaagcca
      661 ggcaaaggcc cggcctttat gcgtaccaag ctgaaaaacg tgcgcaccgg ccgcgtcctt
      721 gagaacactt tccgcgacag cgatactttc aacgaagtgc gcatcgaacg caacaagatg
      781 gagtacctct acaaagacgg tactttcttt atcttgatgg acctggagac ctacgagcag
      841 atccatgtgg acgaggctgt gatcggtgaa cgcgaaaagc tgatgatgga caatatggaa
      901 gtgatggtca tgcgcgctcc cgatggagaa atcctgggcg tggaactgcc gcttaccgtg
      961 atccagacca tcgctgaatg cgagcccaac gtcaaaggca acaccgcttc cggtagtggc
     1021 aaaacagcct acaccgagac cggtttgcgc atcaccgtcc ccttctttgt ggaatcgggc
     1081 gagaaagtca agatcgatac ccgcagtggc gaatacgtcg agcgggccaa ctaattaatt
     1141 aaagaaaagc gctgagaggc gcgaagagga aataatggga aaggtaaaaa gtttttccgc
     1201 caagctggct cacgaaacct cgaacgaagg taaagtgatg tgccccgttt gcaacaccga
     1261 gatcaagaaa gtgaaagtgg tcggcaacaa aaaaggccag gccggttggt caccccgcta
     1321 tgattttgtg aaggtttgca agtgcaacga ggctgatttt ctggctggca agatactgta
     1381 agactatcag ataagctgtg ttaacagaat ccgcgaactc gttcgccccg ggtctttatt
     1441 ccggagcgaa cgagtttttt attgacagcg ccatggcatc gagcaagctg gcgctcgttt
     1501 ccattcggcg tggatgactt gattgcatcc cttgtttgtg ttcttcatca ataaagtccc
     1561 aagggggatt gtggcatgtt gccaatcaag ccgtatctgg tcctgagcct tatgatttgc
     1621 gtggcttcca cgctgctggc attggtgccg ccgcatccga tgtccactca tgttccttcg
     1681 tcctgggaac ccgtcaggat agaagcgccg ctctctcaag acggcagtct gctgaaggcc
     1741 cagcgcgtgc tgcctaacaa catcctggtg ctgagagtgc agttttccga cgtcggtttt
     1801 gtgtcccaac ccgcatatcc tgatcaactg gcgcacgatg acgtcttttt taaccgttgg
     1861 atgctgcatc tgtctgattt ctttgcggat gcctcacact atcagtatga gctgagttac
     1921 acactccatc ctgaggtgat cacacttcct catccgatgg cctattacgg aggagacaca
     1981 tccgaaaaga tcgatgccag aatcgctgaa ctggccaggg atttggtgga cgcagtggat
     2041 gatgaggtgg atttcaacca gcatggcgga ttgattatct ttcatgccgg ggccggacag
     2101 gaatctgaca ttaacgggat caggactgat ctgatctgga gcacctttct gacccgcaag
     2161 actttacaag cggcattcgc gccaaacaac gatagttatc ccggcctctc aactgatgac
     2221 ggggctaatc tgatcaacat cgtgatcgtt cctgagcatg agtttcagga ttacttccca
     2281 gcggaagggc agcaggacgc ggaagcttat ctctttagca tctatggagt tctgactcac
     2341 cagtttgccc atatcatcgg gctgcccacc ctctttgaca acgattccag caatggcacc
     2401 tctcagggaa tcggcaattg gggcttgatg ggaacggggg tctggaacgc cagcggctat
     2461 gttcccgctc agctaagcgc ttggagcaga atgtttttgg gttgggagac gccgatctcg
     2521 gtatcgggtg atgccacagg cctgttggtg gattattttc tggaccacgc gacggggagg
     2581 aaccggctct acaagctgcc catttcggcc acggagtatt tcctgatcga aaaccggcag
     2641 cagaatcccg acggcagtct ggacccctat tccaatactc ccagctacac cttcaagctc
     2701 ctgcctgagg gcgagcagga ttattacgag aactacccgc tgcttccctt ctttaacttc
     2761 atggaaaaca gatacatcgg cagcgagtgg gacttctttt taccgggatt gggaggccct
     2821 gtaccctcgg gtcagatgat gctgcaagac ggttccggcc tgctgatctg gcatatcgat
     2881 gaagccgtta tagctgaaaa cttcaccacc aattttgacc gcaaccgcat caatgccgat
     2941 tcccgtcaca agggagtcga tctggaagaa gccgacggca tccaacatct cgacacggcc
     3001 atctacgatc cttacaaatg gggtagtccg tttgacgcgt tccggcagga caacaacgaa
     3061 tactttggca atcccaccta tcaaggttta atgagccttc ccactgctga gagctattat
     3121 ggcgggatcc ctctcgaggt ctatgatatt ggccctaacg gcaatcagat gagcttctcg
     3181 gtgcgctttt cctggcgtct gagctcagat tacgtgggcc ccaatcccat caatgccgct
     3241 atcctggatc tggacggcga tcaggaagat gaagtcttct acgcgatgcc ggatggagag
     3301 ctatacgcat gggatgatga gcaaatgatg cctggtttcc ctcagcgccg catgccagtt
     3361 acccaaccct atgtgtggga tgaagatcat ctctatctgc ccatggagct tggcccgctg
     3421 gccaggctgt atagaatgaa ctccacgggg ggtcagtacg tgttcacgca gagtggagca
     3481 agctgggcca gtcatcctgt ggatatcggc aaccagctaa tgctgcctct gaatgtggat
     3541 ggcggagcgg aactgcgacg ttatgacaag ggcaccacat ccaccccctt gggactgagc
     3601 attgaccagc ccatcgcgtc aaatctggtg tggtttcgta atcagctcaa cttcctgact
     3661 gccgagccgg gattgtatta taagctatgg acttacgacc cagccactgg gtccagctct
     3721 tcgctaagcc tgccaatccc cgcagattcc accatcgtgg gcattttcaa agctccactc
     3781 atcaaaggca gtgtgaatgg cgagctccta atccagaccg ccaaagccgt ctacgtgttt
     3841 ggcagcgatt ctcagggtct gccggtcttt gtgtttagtc acgattctgt ctgcacggcc
     3901 cctcttactt tggcggattt ggatgtaaat ggaagcctgg atatcatttt gggtactgag
     3961 aatggcgtga tcgtgctgga ttactctggc cagcgcatga gccccgaaaa cctggagggg
     4021 cttagttctg aagagggcgg tttcagtgcc ggggccctgg ctttggatat cgatatggat
     4081 ggtaaaccag agcttctggg caatttcgcc aacaaccaga tcaaggcctg gagcgcaagc
     4141 tttcagctgc tgactggctt tccggattcc ttcgcggaac ggagccgcaa cctgccactt
     4201 gtatccctgg cttcagatgg aaatcgctat atctggagcg ctgctgataa tggcagcatc
     4261 ttccgcaaag ccattccagg acctctcccc aacgacttta acaatatctg gcactgcgag
     4321 tttggcaatc tcaaacgtag cgccagcatc gatccggacg cgctgccaaa ctcgtttgag
     4381 agcgcttccc tctttgtgcc gaacgagctg tacatcttcc ccaatcctct caaatccatc
     4441 tatgccgatc aattgacgct aaatgtcatg accaatcgcg acacccctct ggaagtgcag
     4501 attttcgaca tcggcggcaa agtgattttt gaacagaaaa gtatcgccag ggcctacctc
     4561 agaaacaggg aagcgatccg aatcccggta gagaagttgg ccagcggagt ttatataact
     4621 gtggtaaaag ctggtaaaga aaccaagcgg cttaagtttg ccgtcgagaa ttaacacgga
     4681 gatatcaaat gaacatgaat agatacatcc tgaccttttt gcttctgagc gccacgctgc
     4741 tgggagcgga tttgcatcct gaagccggga cctacgggtt tcagtttctc aatatcagca
     4801 gcgatcccgt ttccatggcc ctgggaggca gaggaatcca tgccaccatg gaccgggcgg
     4861 cttttatccg acaacctgcc gtgggaactg taaacagcca tcgcagcctg ggtgcttctc
     4921 attctccctg gctggacgac accacattca accagctcta ctatggctat tcagaccggc
     4981 tgagccactt tggcgtagcg ctccgcaacc tcgactacgg agagctggaa agcagagacg
     5041 agaccgggac cctgatcggc tattaccatc ccagcgatgt aagcctgatg gccaactatg
     5101 cctggcgttt ggggccttcc acctatgtgg gaatgaatgg cggggtcgtg tatcagaagc
     5161 tcagcaccgc ctccagctat ggaatcaacg tggacctggg tgctactatc ctgccgccct
     5221 tcccaaacag ccttctgtct gtatcggtca gaaacgtggg cgattcttcc aaaatgaatg
     5281 aagtccccac ccgcatgccg gtctcactgg aagccgatct cagcaaacag tggcagatcg
     5341 agcagggctc tctgatagtg gaactcagcg ccataaaagc ggttgacgct catttgaaag
     5401 gcgtggtcag cacgcagttg acggttttgg accgcatcgc tttgcgggga gcatacaagc
     5461 tcaattacga cgcggaaggc ctcagcgccg gcttgggcgt caaagtagga aagctggata
     5521 tcaactatgg ctgggcagac tttaactcgg ctttggacga tgtgcacagc tttgggctgt
     5581 cctggaactt ctgaggtatc cgatgagcag atctttcatc atcctgatcc tgttgggagc
     5641 cttactgggc ttgaacgctg tggattttac ccccaatcag ctgatcttca agaccgcgca
     5701 gcctttgctg gaaatcaggt cgggccggac aggactcagt ggttttgaca gctatctgaa
     5761 tagcctggga gcttatagcc tgagacccgt tacaggtatg ccaggcgacc gttatttcct
     5821 ggtggacctc acaaccgcac cagactggca gaacctccga agcggcaatc tcagcttccc
     5881 cggaatagca tacgttcagc ccaactatct gagcaagatg catatagagc caaacgaccc
     5941 ccgctatcca gagcagtttc actatgttac atccactccc caggcctgga atttcaccac
     6001 cggcagcgaa ctcgtcctcg taggagtggt ggattccggg gtgctgatag atcaccctga
     6061 cctggctgcc aatatctatg tcaatcctca tgagatccct gataatggca tcgatgacga
     6121 cggcaacggt tatatcgatg actggtgtgg atgggatttc tccgatgctc ccgagatgtt
     6181 tgatgtggga atcggagact atgtgggcca ggataacgac cccgaagacg agaactttca
     6241 tggcacccac gttgccggaa ttattggcgc cgtgggaaac aacgggattg gcgtggccgg
     6301 agtggcatgg aatgtgaaga tccttcccat ccgggcgggt ttcaggacca cccaggcgga
     6361 aggttatctg caggatgacg acgcagccgc ggctttgatc tacgctgtag atatgggctg
     6421 tcaggtgatc aacatgagct ggggcgatcc aaattactcc cccatcatcg gagacgcctg
     6481 tcaatatgcc tacgaccggg gagtggtatt ggtggcctcg gcaggcaaca atcccacccc
     6541 caacctcagt tatcccgcca aactcagcac cactctatcg gtaggggcag tgaaccgaaa
     6601 cagaaatctg gctgggttct ccagttttgg tcccgatctg gatatcgtag cacctggcga
     6661 agcggtcctg agcacctaca agctcaccgc tggagagcag tattttgagc agaacggcac
     6721 ctcgatgtcc gcgccctttg tggcaggagc agtggctttg cttctgtctc tgcagccagg
     6781 cctcagtccg gatgaagtga gaacgcgctt gctgacttcc accgatgacc ttgggacccc
     6841 tggctttgat atgtattacg gccacggact gctcaacgtc cgcaagctgc tggaaagcat
     6901 ctcacctccg ctggtgagca tcaccagtcc cattgagcaa caaggcgtaa acggcagctt
     6961 tgatatcatc ggcaccattc aggccgaaga ctttttcaga tacagcgtca tgtttgccat
     7021 caaggatgtt cccgctacga tcctggactg gctggacgtg gaaactcata ccaatatgcc
     7081 cagttacaag actcaaccgg tttcaggtgg agtacttgcc agtttttacg ttccggagct
     7141 gctaccggag cggaattatc tgatcagggt gcagtatgaa gaccgcagtg gcaagaagta
     7201 caacagctat gtctctgtcc aatatgacag cagtccaccc ctgttgagaa ctgaatccct
     7261 gcagggccat agccgttatg acggacagga cctgagatat tatctgacgg ctgccttcaa
     7321 tgaaaaagtg cgcaccgagc tgagaatcac cgcctcggat ggcagtgtga gctaccgcta
     7381 tggaaccatg gctgacagca tccagatttg ggctttgccc cgcaccctgc ctgagggcaa
     7441 tatagacatc cagatcaagg ccaccaacgt ctgcaatctc agcgctttat atggtgatta
     7501 cgaagatgtg ctcaacatca gctatgatat catccccaac cacggctacc tgcccacaat
     7561 gattggcaaa gccagagtac ccttgccccg caccttcgat tttaacggtg acggagcgat
     7621 ggaatatgtg gctatggacc tgcccaacag cggttatggc aatgtgagtg tttaccagcc
     7681 tgaagcgggt ggtcatgtcc ccaagcatga ctttatggat agcttctggc tgctagacac
     7741 aggcaacacc aatacccagg ggcaggaact gctatacatc aaaggagata cctgcttcct
     7801 ggaagagact caggagaccc accaataccc caacctggtg atctgggaag aagcttcgat
     7861 ctcgggtgga gtgattgccg actacagcgg tgataacaga agtgatctcc tcctggtaaa
     7921 aaacctgcct actgaacaag tggtgcaggc ctataaacgc ggcacgggag ctaccatcac
     7981 cgccaaaaac acccttagaa acattactcc caccaacgtt cgcaacacct tcgtgcccac
     8041 catcattgtc gaaagactgg atgatgacaa ctatccggac atcctgaccg cggataccga
     8101 tggcgacatc atgatctttg aaatcatgaa cgacaacatg gatgccatgg tgtggcatca
     8161 ccgcatgccc gtcggaaaca cctacaccgt caccagcggt gactatgacg gcaacggtaa
     8221 acaggatttc tttatcggag gctactacac cgatatcctc aatcccgata tgaacttctg
     8281 gtattttgag ggcttcaaga acgtcgccaa tgaccaatac agcagcatgg gctctctgtt
     8341 gttcaacaac gtgatgtctc aaaacgccat ccagtccttt gatctggaca acgacgccaa
     8401 ggacgagatc attctgggca tctcgcccaa cctctacatc gtcaaatatc aggaaggcaa
     8461 attccagccg gtcttccagg gaagcagcat gcgcagctat cagatcatgg cctggaagga
     8521 tgccaacaac cgtccctatt tcaccaccaa ttacgcggtg ggagattcca cccaattcgc
     8581 gcagtggacc tctgaagatc cctacactgg ccctcccact ccagccaatc tgcacaccgt
     8641 accaatcggt tccagctctg ccaggatatc atggaacgac agcggagcgt cctattatcg
     8701 cctctaccgc atgaatgaag ccggcgaaac tgagatgttt gacaatctca cccagaccct
     8761 ttatctggat ggcggcctga ccgagggtca aagctatctg tatgccgtca cgggagttga
     8821 ccctgcctac aatccatccg aaagcatgcc cggacagtgg catgccgtga ctcccatgaa
     8881 ggctcccgaa ctggttgaaa tgcagatggt ctcagcctct gaaattaagt tgatctatga
     8941 tcaaatacta gcccccacat ctctaaatcc aggcctgtac gagttggatc acggaatggg
     9001 taaccccatc accatcaaca gcatcgccag ccagacagga gtgttgctaa gattcaggca
     9061 agctttaccc gagatcgatg gtctgtttag cctcactctg catcagatca ctggaacaac
     9121 cggtgtagcc gtgtctcaga accttctgca attccccttc gctccggata ccgaggcccc
     9181 ccaagtggtc tcggccgaag taatccaggg taacagctcc gtcagaatcc tcttcagcga
     9241 accgatcgga gcagctcctg atcccagcta tctgggcaac tataggttac atgctcccgc
     9301 aaacgatcag gaaaaccgga tcagctcggt acagcatcaa aacgacagcg tggtcctgag
     9361 cttcctcacg cccctcaaat acagcaatca ggcctaccag gtctttatcg aaaacattca
     9421 ggacctggct ggcaacacga tttctcccca gaacagagtg gccaggatca agctcagcga
     9481 cgttaacgac ctcaagaagc tgaaagtata tccaaacccc gtaaaaggct ctgagcacaa
     9541 gtgggtgtca atccacaact tcccctttga gaagagcggc aggatatcgg tttacaccag
     9601 ctcaggagat ttggtctaca gctccgggct gggacccttc caggcagtgc tcaacaacaa
     9661 tatctggcgc tgggacctca gaaatgacca tggccgcatg gtatcatctg ggatctattt
     9721 ctatgtggtg gagatggatg gagagatttc gcggggtaaa atagccatca tccagtgata
     9781 aggacgctgg cgacattgct gactacaaac ctgcagcaat cgagcaacag agcaaagata
     9841 cctggcttca aggaaaagca gcgctcaggc aaccggtggt caaaaaaggg gggttgaccc
     9901 tacaccccta tcttggcaca gtattcatag acaggacagc tggaacattt gggcttgcgg
     9961 ggcgtgcaga gattctgtcc aaaagccacc acatgggagt tgtactgcaa ccagtaccgg
    10021 tctggcaatt tggcacgcag ggccatctca ctttccaaag gcgactgggt ggagatgtag
    10081 ccccagcggt tgctgatgcg gtgaacatgg atatccacac agatggccgg cagtccaaaa
    10141 gccacggccc tcacaagatt ggcggtcttg cgtcccaccc ccggaagctt gagcagatca
    10201 tcgatttcgg aaggtacttt gccatcaaag agatcatcca gcactcccgg aagctgcttc
    10261 aggtgtttac ttttttcgcg atgaaagccc acctgccaaa tgaggcggtc cagctcttcc
    10321 aatcccagtt tgttcaggtc gtgatgcttc tctactaccg cgaacagcgc ggttgccgct
    10381 ctggatgtgg tttcatcctt ggtcctggcg ctgaggatgg tagccaccaa aaccttaaac
    10441 gggtctttgg tttgaatctg gataagatct accacgggtg ttttgacttc cacaaagtgc
    10501 tttgccagcg cggtcattac ggcatcgata tcaaacttgt tgctcaattt ccactcctcg
    10561 gggcataaac ccgcaaaaaa tatattgcat ttcatccgcc gcccgtattt ctgtcaacag
    10621 taaaggagat acacatgtgc ggaagatttg cgcaagtgat caaacatgat cagttgcaga
    10681 agctggagcg ggaactgcgt ctcgatcaca agagtgatca gttgcaggcc aatttcaatg
    10741 tggctcccac gcagacggtt ggcgctttcg tgagccagga ttccggcagg tatctggggt
    10801 tctttcgctg gggtttgatc ccctcctggt ccagagaact gccaaagttt gagctgatca
    10861 acgcccggtc tgaaagcctg atggaaaaag ccagctttcg gggtggcctg caaaggcggc
    10921 gctgtctgat cccggctacg ggattctatg aatggcgcaa ggcggaccgg cagccttttt
    10981 ttatccaagc cgtgagcggt gaccctctct tgatggcagg cctttgcgat gcctggaacg
    11041 gcccggatgg atcgtatctg ccctctctgg ccatcatcac tatggatgcc aatgcccaca
    11101 tgcaatctct gcatcatcgt atgccagtga tgtttaacag tgaacaagct ctggactggc
    11161 ttgaccataa aaaccaggac cccgctcaga tgaacaagct gatcctggag gcctctcagc
    11221 aagctttgga tatttacccg gtgagcaaac tggtaaacag ctccagggcc agtggtcctg
    11281 agtgtctgga acccagtgga gacagcgctc cggatgagca agacccctcc ctgtttgatc
    11341 ttttggatga gatatgattt tggacagcca agcctctgta cttgttatcg atgacgatcc
    11401 tcacctgctg gaactgatcc gtgagcagct cagcgaatcc ggagaatacc tggtcgtcgc
    11461 gctcagcgat ccgaaagaag gactggcctg gcttatggac aatcaggtgg aggtggtgat
    11521 ctccgatatc cacatgccag gcatgagcgg tatcgacctg atagatcata tcaagctata
    11581 caattccagc atccaggtga tcctgatcac cgggtatccc gataacgcct acatgcgttc
    11641 agccatcaaa gccggcgttt ttgatttcct gcgcaagccc tttcccatca ccgaactgct
    11701 ggtgagcgtc aaacaagcca gggaaaaata tctgctcctg actcaaaacc tgaagtatca
    11761 gacagaactg gaactcatgg tggtggaacg cacccgccag ctgcacgaag cgaacctcaa
    11821 gctggaaagc cattatatca acaccctgcg ggccatggta aacgccatcg aagccaagga
    11881 tgtttatacc aaaggacatt ccgagagggt gacggcactc agcatggtac tgggcaagtc
    11941 catgggtcta agtgaagaag agctcagaat cctgcgcttg ggatcgctgc tccacgatct
    12001 gggcaagatc ggagtctata aaaccgtgct ggacaaagcc accagccttt cggacgctga
    12061 gtatgagctg gtgcgccatc atcccctcat cggagcccgc attattgagc cgattggact
    12121 gcccaaggaa gtaggcatgg tgatcctgca gcaccatgaa tggtataacg gaacgggtta
    12181 cccctttggt atggtaggcc ctgagatcca tcctctggcc aagatagtag ctatcaccga
    12241 cgcctttgac gccatgacca gtaaaaggcc ctatcgtccc caactcgatt acgagcaggc
    12301 cgccaaagaa atcgaaagac aactcgaggt ccagttcgat cccaatatcg gccagacttt
    12361 ctgcagcaat ctccaggagc tcacagccat cctcagcaat cccaacggtc tgagcgacta
    12421 tgtgtttggg gcagacaccc aacctgcctg actgatccct gcccattcat ccgccgtaga
    12481 gataccgtgc gctctcccgg gtttttcgcc ggagagcgca cggtatctct acggcaggtg
    12541 accaggcaag catcaggagg taacaatctg aacgggggga caatcacaaa gatttggttc
    12601 aaccccgccc agagccttgg aaaaggggtg gaattatggt gccggagtgg cttggggagc
    12661 tatttccaga ttgatggtca ccgggccgtc attgagcagc tcgacttgca tgtccgcggc
    12721 aaagatcccc gtctgaacag ggacgcccgt ctccctgagc tttgccacaa aactctcata
    12781 cagctctctg gccagcgagg gaggggccgc ctgggtaaaa tccggtctgc ggccgcggct
    12841 gcaattggcg tataaagtga attgggagat cgccaggata gagccaccga tatcgagcag
    12901 ggagaggttg agcttgccgt cctgatcata gaatatcctc agctccaata tcttgcgaat
    12961 ggcaacatcc agcacggagg agtcgtcttc gcgtccaaaa cccacgaaga ccagcaagcc
    13021 ttgcggaatg gagcccacca cgctgccctc cacgctcacc cgggcatatt ttaccctctg
    13081 gagggtgatt ctcatcaatc gatccggaag tggtgcacct tccagcagcc gacgtcatcg
    13141 atccagatcg atccgtcctt gaattgtggg atcatgatcg ccacccggcc cgcgttcaca
    13201 cccggacgga tgaagcccgc gctgatcgag accttgttcc aggcctcatt aggtaagatc
    13261 ttttgtttga agctgttata gattttgcca ttttctttga aagtgatgaa tctgagggtg
    13321 atctccggca tgtctttaga agtgcctcta atgcctgccc ggatatagta gccgccgtag
    13381 cggatcacct tgaagggctc ggagatcagg ataaccgcgt tttcagacgc gtccacccgc
    13441 aaggaagtct tgccctcaag agctacttcg gtatcgatta agaccttgcc atccgggctg
    13501 ttgggtggat cgtaatccac tgcccagcct ttgagagcgg tgcggggatc gagtgaatat
    13561 ggattaaaac cgggattaaa gaccaggttt ggcacgtttt gggtctgcga ttcagtgtag
    13621 gagaggctgc tgctcacgaa acatgagctc aaaatcaaca tcaggagggc cattaaggcc
    13681 agcaagtact tcgtcatttc ataaatcccc gttcatttag atattttagc acgaccccca
    13741 cggccagggc gttgatcagc agattggatc caccgtagct gataaacggc aatggaattc
    13801 ccgtggcagg gaccacacca atattcatgc cgatattgat gaaggtctga aacataagat
    13861 aagccagtat tccagcgctg gcaatctttc tttctttgac tttgatctcg cccatagcgt
    13921 tgatcaagcg caggaaaaac aaggtaaaca caaataacag caaggtgctg cccacaaagc
    13981 caaactcttc accgatcaca gagaagataa aatcggtgtg atgctccggc aaaaagttca
    14041 tgtttttctg ggtgccctgc agccagccct ttccaatgat cgcgccgctt cctacggcga
    14101 tcttggactg gatgatctga taacccgccc ccagaggatc acgcatgggg tccataaacg
    14161 tgaggatgcg gttttgctgg taatctttga gaccattcca gaacaccggc ataatcaacg
    14221 ctatgaacac gttgacgata cccgcgatgg tgatcgccac ccaggatagc ctcaccctga
    14281 gcagcagaaa aaccagcaag gccagccaga tcactaccgc cacccaactg agggacgaca
    14341 cgatgctcac caccggtgag accatcaaaa ggatgtaaaa cagaggtacc tcagccgcta
    14401 ccagcatgga cagcaacgca aacataaaca ccagagtcgt accaaaatcc ggttcgataa
    14461 tgatcaatag caccggcacc aaaaccagtc ccactccata gaacacctgt tttagctcgt
    14521 tgagatggtc tttgctgatg attctcgcca ccatcaggat ggtaaacagt ttggcactct
    14581 ccgagggttg ataattgagg cccaaaaagc tgaaccagcg gtgcgaacca ttcacggccg
    14641 gggtgaacaa gaccagcacc agagcgagca gattggcaag aaagaggggc aggaccacga
    14701 tgtcgaaaat cggcatgggt agtttgagca gcaaaagaat cgccccgata gctatccccg
    14761 cgaagataat ctgcttccac cagtggtttt cggtgctgct gtgttgtccg atggtggtgg
    14821 tgctggcggt gaagatagcc acgcaaccca tacagatcaa gatcagcaag agaaccgcga
    14881 gattccagtc aaatttcttg cgattgaaca ttagtcttcg ctctgagttt ccatgtgttg
    14941 tcctggttct ggagctggag attccgtggc agcgggcgtt tcaggttctg tgacttcctc
    15001 tctgtccact ggtgccaggc tacggaactg cgcagggacg ggagcgggtt tcttgaagct
    15061 ttccaggttg cccacgtaat aattcatgat ccggttgcag ataggaccag ccacagcgcc
    15121 tcccccacca gcattttcca gaaagacggt aaccacgatc tcgggcttgt ccgtgacgat
    15181 ataaccgcag aaccacgcgt gagttacttt gccaagggcg ttttcggccg atcctgtttt
    15241 accgtatgcc gtagctcctg ccacattcaa agccctgccc gtaccgccgg gagcgttgca
    15301 aaccgcccaa aggccatcct ggatgcactt tacagtgttg gcagaccagg gcagacgtcg
    15361 ttttacaata ggctgcacct gttctctgtc cagcctgcct tttcccagtg tttttaccaa
    15421 taggtggggt tgaatccaat agccgcctct ggcaatggcg gcatataaag tgttgatctg
    15481 cagtggagta gtgagcactt caccctggcc gatagagagg ttggctttgt agccttgcaa
    15541 gccggtgctg cggcccaagc gtttctgata ccactgggtg ttgggataaa ggccgtttct
    15601 ttcgctgggc agatcgatcc ccgtgcggtc tattactccg catgccttgg catgccgcca
    15661 ggcatcatca agtttgatgc ggctgatcag gtcatagaaa aacacatcgc aggaaacctt
    15721 gagagcgtcc acaatgctcg tccttccatg gcctccgctg gaccagcatt tatagaagcg
    15781 gttgccgatc ttgaggccgc ccgcgcatga tgctaacagt gtgttccggt ttatgatccc
    15841 cgtctccaga ccttccgaag ctgtcaccgg tttaaacacc gatcctggag gataagcgct
    15901 ctggcaaacc ctgtccaaaa gaggtttttc cggacggttg agctgagacc acagctccgg
    15961 actgatcttc tgcatgaaga cattgggatc gtaatcgggt ttactgacat aggcgagaat
    16021 gccgcccgtc ttgatatccg atactactat tgccccgcgg gccccgactg ggaaattgtc
    16081 actggcaaat tgctgcagat cgttatcgat actgagcacc agactcaggc cgttcagagg
    16141 atcgcgggaa ccatctaccc tgaaaaggtc caggcttttt ccgcgcgcgt caacctgcag
    16201 gatttctctg ccatccgtgc cgcgcagcaa cacctcgtag tatttttcca agccgttctt
    16261 gccgatatag ccgttcagag aatagtcctc atcccggtag cgtttgaatt ccgcttcatt
    16321 gatgcgaccc acgtagccag aaaaatgatt gggatacaga tattgacggg tggtgccgtt
    16381 gcggaacacc agttcgggat agtagttcat cctttcggac aaggccagca cggtctcata
    16441 gggaatgttg tccaccagca ggatctcttc gtaggtctta aagcgttgct ggaataccag
    16501 atcgtgcagc tcctcctgat tgatgccgaa atagacgttt aaaaacagcc cgagggcctt
    16561 gatattggtg attttgccgc tgatcagata gaggttgtgg gaaggtatgt tgatcgcgac
    16621 ggggcgatac ttgctgtcgt aaatctctcc ccgggtggcg gatacgcgtc tgatacggac
    16681 gaagttgctt tccgcgatgc ggcgatattc atcgcccttg atcacctgca gtctgaataa
    16741 cgcgccaagc aagaacagaa acaacacccc cagcgcgatc ctggagacca gcgtgggaat
    16801 gcttctattc acttacaatc accaccctca tccttgagag cagttgcaga gaccaaaaca
    16861 ccacaaaact gatgaccagg ttgtaggcga aagcgatcag gttcagagtt gcgagttggg
    16921 ccgtaaatcc atgcccaaca cccaatacca gccacattac cagatgatag atcaggtttg
    16981 ccagcaagag ggtgagcatc ctggccacct tgctctcagc ctcaaaaggc ttgcgaaact
    17041 cagacagcgc cattcccaac agcagaaaag taagcggtga cagcccaaat agggaaggct
    17101 gggtgacgtc atacatcgcc cccactatga atcccactcc taaagccatg tctctggggc
    17161 ggctccagat cagccagatc atccagggca ccaggatatt tggaatagtc cccgccagtt
    17221 caaaagctgg cataaccagc agctggacat agagaaagaa aatcccggtg aggtaggtcc
    17281 acaagaattt ccagatcact gttgccgggc tccctgtccc tgaggagtgg ctagcgtccc
    17341 catttgcttt cttaagataa acacgtgttc caggttttcc acctgagtaa agggattgag
    17401 ctccgcgctg ataaacaagt tgtcgctgga ttccttgatc cggctcactg aacccaccgg
    17461 atatcctttg ggaaacaatc tggaaagatt ggaggtcacg atcgtatcgc ccacgctgat
    17521 ctgggagccc agcttgatca tgttcatgct gatcacgccc ctcaggtcgg cctgcagaat
    17581 gccctggaca ctggtgttgc gatccatgac ggggatttga aactgggggt ttgaaaatgg
    17641 cagcaccaca caatatgtat ctgccgcgtt gatcaccttg cccacgatcc cggaagctga
    17701 aaccaccggg ctttccggtc tgaggccgtt gagtaaacct ttgttcacga tcaggttgcg
    17761 ttgttgaaac tggcccgcgt agccgatcac ttcggcaatc tcaaaatcca tcaaacccgt
    17821 gtcgaatgct accgcggtgc cggaactgaa catcttcagc tcgttttcca gggccagatt
    17881 acggagggtc tgatccgcca attgaactct cagccccgcc acttcttctt tgagcgcgct
    17941 gttggcccgg atggcactga tggagcgggc aaagggataa aacaccgtcc tgcccagcaa
    18001 atctgccctg gcaatcctgg cttcctcggt cccgatcatc ataacgagcg cgaacaggag
    18061 caggaccagc ggaatgaggt actttttgat cacgccgaaa ctctcacagt catcctgcca
    18121 ggtgcagctt acagcagtct ggaagcacgc tttctcccga aaccgggatc tagtcctcga
    18181 tgcgcttgat cagcacttcg cgatagcggt ccacgtcgtc caggaccttg ccggcgccct
    18241 tgaccacgca ttccagcgga tccgggacca catgcacggg tagatcgaca gtcttgcaga
    18301 tcttttcgtc caatcctttc agaagtgcac ccccaccggt gagaaagatt ccgcgttcgg
    18361 ctatatcggc ggaaagctca ggagcggtgc gttcaaacag acgcttgatg gcgtcgatca
    18421 tggtggagac ggtctcagag agggcttcgc gcacctcttc ggagctgatt ttgatggtaa
    18481 cgggatatcc cgagacgatg tcacggcccc gaacatccat agtgagttcc tgtttgagcg
    18541 gataggcgga acccacggtc atcttgatcc tttcggcagt ctgcaggccc acgtggaggt
    18601 tgtttttctt gcgcagatag ccgataatgt cggtatccat tttatcgccg cccacgcgga
    18661 tggagttgtg gaccacgatg tgagacagtg agatgactgc tatttcagac gtcccgccgc
    18721 caatgtccat taccatgttt ccacaggctt gttcgatggg cagatcagca cctatggccg
    18781 cggctacggg ttccgagatc agaaatactt cccgggcgcc ggcatggaga gcgctgtccc
    18841 gcacggcgcg tttttccact tccgtaatcc cggaagggac gcacacgatc actctggggc
    18901 gcaccaggag gcgcttcttc tgggccctga ggatgaggtt gcgcagcatc agctccgtca
    18961 cctggaaatc ggctatcacg ccgtctttca atggtttgat caccttcact tcgtcagggt
    19021 tcttgcccag catcactttg gcttgctgac caatggcaat gatctttttg ttgtcagtgg
    19081 acacagccac cacggaaggc tcgtcgatca cgatccccga gctctttttg taaaccagcg
    19141 tgttggcggt gccaagatcg atggcaatgt cattggccat gaggccaaaa aagtcaaata
    19201 aggacatcca aaacctctct atttaaggac tttagcctca ggcggcagtc ctgtcaaatc
    19261 ataaatttcc tatagtttca cggtttttca gaaggctctg agtgtcaaga gaaaatgctg
    19321 tttgtgcgct tgttctgtgt atgaaaccgc tttagtccat gatgctgaac tttgccatcc
    19381 tgacctggcc tccgattttg acgctgcaaa gatagagccc caccggtaac ctgggaagtt
    19441 ccagacccag cagcgctttg ccgtcggtgg tgctaagtgt ccggctgtaa acaagctggg
    19501 cccgcatgtt gtagatattg acgctacctg aagcgcgacc ctccagctct gggatccaga
    19561 cgctcaaacg cagactggga gatttggtcc tgaggggatt gggatagagc ctgagacctg
    19621 gggtgaaggc tgccatggct tttccaggct gggtgatttc gccttccgga tcgatgggat
    19681 ctggcttcaa ggcctccagg ccaggatagt gaaccggggt cctgccgcct tcaaaaacca
    19741 ccagttccag cttgttcaga gaggcatctc cgggctgggg atagagggta cggatagccc
    19801 aaaagctgtt ttccgaccct ttgtaagaca gcttgtaatg tgagttcagg cccgtaagag
    19861 gagtgtttct gaaagcatag agcttctctc catccgccat gatgaaattg aagctgggag
    19921 ctccctcata atcgatccat ggcatcagtt cccacagcgc cgctcccaat ccggccagga
    19981 tatcatcgtt gtttagacgg atatgattga tgatgaagtg gaaaagcagc tccgaatcca
    20041 cccactgaaa ggggtctgag agaccaaaga agtcgctggg atgattgacg aaccagtttt
    20101 cgtcaggatt gatctcccgg acgcgctgaa tcatgaagtt gcgggcgctg tagcagttgc
    20161 cgttgtgcat gaaggccagg ctgcggtcgc gcagatcaaa cacgaaggga tgattgccat
    20221 agctgagagc gctggcgttg cggccatgga tcatcgcgat gtgactctgt gagttggtcc
    20281 ccaaaatgct gtacatggcc ctgtccagca cgtcaagttc ccaaaactgg gagctgtaat
    20341 ctctatccaa atacctgccg gtgtagtatg tgttgccgaa gtccgtggac tggaggacgc
    20401 gtttatacca gcggtcactc tgagagagga agaggcgtcc ccgctcgtaa gccacagtgc
    20461 cgtaaccgtc tgaatttcca tagggctggc tattggccat cacgaatccg aagtaatccc
    20521 aggggtcgtt gtaagcggtg tactcaaagg gaaaaccgct ccttggaaag cttttaagcg
    20581 gccgcgctga gatcatggcg ctcatcacgc aggaaggcag atcggaccag aacagcaacg
    20641 agaagagcag aaacagacac gcttgaagcc atggacgctc aaaagtgcgc ccgatcacag
    20701 cggtccggcc aagctctttc atcattatca ccgtttacac cagtagccgg caagccgttt
    20761 ggagtcaagt aaaaaagatg cgtaagctac tcgatcttga tcctgatacc ttcagaatgg
    20821 gccgtgaact ctggagcata catgcactgg atgctggtga tcccgtttga gaagtcaccc
    20881 ttctggaaag cccgcatgcc atattcaaac acgtaagttc ccttgggcag cctttcgata
    20941 aagaagttgg tggcggcgtc tccggtggcc tcgtaatagc ccagaccgtc ctgccattta
    21001 tatctggaga gcacgttgag gggctcaaat ccagaggcgc gcaggtcctt catgtgcaga
    21061 tactccattt ctctatctac cctgagctcg atgcgcacca ccactttgtc gcccactttg
    21121 atgcggttgt tgtcgcggat gggatccagc acgaggccgt gatcggtggt ccgctcgatg
    21181 aagagctgct tggtcagctt gagtggagtt tcggcaggcg tgatcttgtc gaggtcttca
    21241 aagtactgcc agtagagggc tccccaactg ggcccgggat tgggattgag gacacaaacc
    21301 tctcccatcg caggtttgat atcgcttccc gtccaggagg tcttgatgta gcccgtacca
    21361 gcctcgggcc aagagccatt tttggggctt gggtccagct tgccgccgcc cacagttatc
    21421 tcagctaatt gggaagagcc cagccacgca gttccttcca acagaagagc atagcaggcc
    21481 tgagcggtgg ccttggtggt tttccagttg ttggtctgtt tatttttcag cagccaggtg
    21541 cgcatttcgt tcacagtcag ggtgtccttg gccacttcgc tgaaagcctc gatcaggatg
    21601 gcctgacgtt cgataggtgc ctgataccag taccatcctg agttcgcttt ccaccacatc
    21661 cccatctcct catcgctgag ggaatgttca cgcaaggaag cgatgatgtc gttggcagtc
    21721 tggagtttgt ctttgcggta gagggccagg cagagcatgc cctggctgta gaaatcccgc
    21781 tggagccagt attgctcggc ctgagaccaa tagtatgaaa ccgcttcctg agcgtaggac
    21841 ggaatgggaa gatcggggaa gaaactgcgg gcgtagagat agtggacagc catgtaaccg
    21901 agattgtcct ggtccagcag gccgtgggct ttcagccact gatagctctc gttgagctgg
    21961 ccgtcgatgt agcccacagc cttgctcagc atggtccaaa gcccgttgtc attgcgcacc
    22021 gatttcacgc ccaggcggtc cagatgccca aagccttcca cgatgtactg cgtgacccac
    22081 caggaatccg gcatcccggc aaaccaggac caggcaccgc tacccgattg attcttttgc
    22141 agttgcgcca gagccgtagc ttcctgagcc ttcagattgt tcagctcaaa aagcaggcca
    22201 agccgctgtc tggcaatggt ttcgtccttg gcatccatca cccagggagt ttcctgcagg
    22261 atcactgcct tgagttcctc gttcttttgc aggttggaaa gcagcgttgt ggaggcggga
    22321 gtctctcccc agctctcaaa caccctcttg atggccggat cggaattggc gatatgggag
    22381 gccaggctgt tggcgtagag cctcgagaag atctgctcgt tgcattcgtg ggggtattcc
    22441 atcagatagg gcaaggcctg aacggcatac caggaggggt tggaagtgta ttccagagtg
    22501 agacggtggt gccggatgct tttggaggcg gagctggcga tcagcttggc gaacctgaag
    22561 tcccgc
//
LOCUS       DAOSFN010000019        22500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1844_length_22500_cov_10.204945, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000019 DAOSFN010000000
VERSION     DAOSFN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 22500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22500
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1844_length_22500_cov_10.204945"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLO35_02480"
     CDS             <1..304
                     /locus_tag="PLO35_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78559.1"
                     /translation="GQATGQALYAKKQETARLIEELTQVQIGIIRTCTEPVSLQEIKK
                     SQNLVSRDFLRVRHLNPLLEDGIVLMKYPDKPKHPKQKYYLSDYGKEVLQAIPKEH"
     gene            943..2202
                     /locus_tag="PLO35_02485"
     CDS             943..2202
                     /locus_tag="PLO35_02485"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOH78560.1"
                     /translation="MSSLAVLGCMWGDEAKAKIVDFLGVSADVVVRFQGGANAGHTIV
                     VEGRKYVFHSVPSGILYPKCKCVIGSGVVVDPFALREEILALREAGISFEHRLFIDDR
                     AAMVLPLHQRLDLMQEKSLGKSRIGTTGRGIGPAYSDRVSRRALRLADLLWPDWLKQR
                     LSALYAHHGLKVSKAELKGEVQVLQELYLFLKPYLAQTDSLLWDWHLQDQSILFEGAQ
                     GSLLDLVYGSYPFVTSSHTLSGGISVGTGLPPRSLDKIIGVYKAYSTRVGEGPFPTEL
                     CDELGDRIRVQGNEFGSTTGRPRRVGWFDAMAARFTARLNGIDSIALTLLDVLSGIEE
                     LRVCTGYWIGGQRLERFPSHHLDLEQVQPEYLSLPGWDKDISHCRSLSKLPRAAKDYL
                     EAIQDLIEKPIELVSVGKERKQTIVIK"
     gene            2215..3384
                     /locus_tag="PLO35_02490"
     CDS             2215..3384
                     /locus_tag="PLO35_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78561.1"
                     /translation="MKHFSLLILLLIASALSAQAADSLNFSLPQNMDPVFEYYSRNYL
                     STRSMGRGTTGVASLGGVESVLANPASYQIDRDAMYMELLIKPPVSTAIYTGDNNFKS
                     PVPFGLVAAGGNVTDDLTWGAAYSLPKSLVMDAFNLEMNLGGYLHTRYPVYNLHQLTA
                     NLGLHIGELNLGLNVHNQFHYISDFTVLRTFERIREVKYLLRPQMGMLWNTQKLNLGF
                     SYTPQSPFNWDLKYVMYETSLPGEASVGLSVRDANRRYNLEMDWTDTSILNSHYKDRY
                     TYKAGFEADVRKFTYRLGYLYRPQIWDGLYQLPVNTTANADTALWWDDAALGGRVPAN
                     DQHLATAGLSWFFKDGCINLGLLYEVSGPAQMAQVNASLGLNFDVFKRRKHPAEK"
     gene            3381..4505
                     /locus_tag="PLO35_02495"
     CDS             3381..4505
                     /locus_tag="PLO35_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOH78562.1"
                     /translation="MKARVQIRKLEGYEPEPLREAVASFLKTVRNPKMSKAKRVLIKP
                     NLLGAHAPEKAVTTHPMVVEAIIRHFLDKGKEVWVGDSPGGTVPVETVWQTCGYADLA
                     KRYPIKLVNLSTLGFKELRHGENTVKISEAFWQCGIVINVAKYKTHSLMAYTGALKNL
                     YGLVPGLVKSDYHRQHPDTNSFAQLLIDLWALTRSRVTYSFIDGILGMDGAGPAAGRP
                     RNFGLFFGSDSIPALDFVASRMMGFQLKDIPYLSEALQMDGILPSRISIPASFRDYPS
                     LNAELGVVKLRKDLLKYVPSFARKGFQKVYYIHPVIGPDCQRCGICVKSCPVKAISWQ
                     PDGFPLIDQQTCIKCMCCHELCPHHAVEINRSLLARMVQQ"
     gene            4502..4840
                     /locus_tag="PLO35_02500"
     CDS             4502..4840
                     /locus_tag="PLO35_02500"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOH78563.1"
                     /translation="MKPRSGKPNPLLRLVFYAAMLFVVSWILLWGEHSFLRTWNMGRK
                     VQALQKEMQELQTINEELKLENQRLKSDPRAAEKVAREKFGLTKEGEKVFRFIPSGPA
                     AKSEAKADSQ"
     gene            4837..6483
                     /locus_tag="PLO35_02505"
     CDS             4837..6483
                     /locus_tag="PLO35_02505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOH78564.1"
                     /translation="MTAIQDVPTLSSVLPEQQQRISSLMGKLLYDTDQERLKHISELV
                     DMIDAEEREGNHGQAFREYSALFMDSLITLLTGGKSSHRSTLFEFMDLLNFLPISPKH
                     RRYTLLKAALDPYLAEYPDVYERFDQYQALLLLTRLGDYENGARLLDELADGVDISRP
                     QVYLIYHLAKARVLYQQERYLEFVGHWLDLIPRFYQTDSADLAVYLLIVWSRTMNWQN
                     DSMLLKALLEKISRGLSHQQNLNMAMATYDRFCLENRLVGPDDKIQLAERLIKRFSSH
                     LSSRQLQELHFFAGNYSSGMRSSFLDSIQYFKYSNYYLNRCWNEQTEGARFLRGILSP
                     EHFCMAMPYIEKRIQVMGNQVSMHNNSYVESLQADYSKIESLLQQVEDLSITDSLTGL
                     RNRRFLEVNIYQLLLLATRQKAPISFAMLDIDHFKKVNDTWGHQAGDQVLKELACILS
                     EEFRKSDVIIRYGGEEFLMVLFDSPLDATLVKMEILRAKVETHPFTYKGRRIHVTVSI
                     GINRNNIVIYNEQDIRTNIDIADAALYKAKNEGRNQVRVM"
     gene            6612..7715
                     /gene="ugpC"
                     /locus_tag="PLO35_02510"
     CDS             6612..7715
                     /gene="ugpC"
                     /locus_tag="PLO35_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOH78565.1"
                     /translation="MAKITVKNLNKYYDNGFHAVKDVCFEAEDQEFMVLVGPSGCGKT
                     TVLRLIAGLEEISSGEIWIGDKLVNKVHPKDRDIAMVFQNYALYPHMTVNENMAFALK
                     LRGDDKATIKKKVDHAASLLGIESMLKRRPGQLSGGQRQRVALGRAIVRNPKVFLFDE
                     PLSNLDAKLRVQMRSEIVKLHRQLENTMIYVTHDQVEAMTMADRIVVMKDGVIHQIDT
                     PLNIYNRPANVFVAGFIGSPAINLVSGKLANLDGGLGFVSAGLKIKLTPQQAQTLNAY
                     ANKAITFGIRPEDIYDARYDSMAEHPQGIDTVCDIVEPLGNEYHLQLKAGEHDLTARF
                     DPKELPKAGQTLKVSLDMGKAHFFDPESELSLI"
     gene            7733..8491
                     /locus_tag="PLO35_02515"
     CDS             7733..8491
                     /locus_tag="PLO35_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78566.1"
                     /translation="MRLTIIIMLALCCGWVAAHADLSEETASANALSGVTALSFSPAD
                     RILSPLVLGSGTVFTYHLPFGDGDTQVWGFHNGSRLSIGHSGFGVSWLSHPDYLWRDH
                     YLSYALGSRDFALGYTQHLLYESFSSGESYYTWSGDLGLGFYGGDYGSEVRWLRIGSE
                     DAQWHFTAITYLSEYSSIATDYVYQEHGKDSVRAASSIGISDLFAIQTSWQSDPPRFG
                     FGTRLKLSYGTFMYAVRTHPEMGLSHSVDVGFTW"
     gene            8494..10002
                     /locus_tag="PLO35_02520"
     CDS             8494..10002
                     /locus_tag="PLO35_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78567.1"
                     /translation="MLFLALLLLPLALVAQSEAELIELNAETLEFNAWQELYRQLLSP
                     GSELGLDNRLWLTDQSYSLHRLMFRHQGRELALNLYNDWTDGDTDANVSIRLAQDKLL
                     RDVALGHYRLQFGTGISMGSGSRSLKGPVLQIERPPSPRRYSPFGAAIKTQWKQVAGL
                     LFASVAAREARLNAASEIISLPRTKTGLDGSTRENLWGAALSYEAKLFRLGVIAYRQS
                     YDRDFEGGLERVSTAGSVYGALRFKAHTLDLESGLTNDSPHHYLAWNYRHQGFEQTLS
                     FAIDPDQDQVAYSTPREVLSRDPDTMEMAWDARFPLFKDARLGLRFAADHLRGGSVST
                     NDLRTRLLASFEYGQQAENFSFRVSHFDREVLSHIDEDYNLSRPKHWRFELAYKDQVL
                     PYLGLMLNCRYHIEDKSSWKDNGLYYHSALQFQSGKLSARLGYQGWQSSKTGLWYEDD
                     TLSAWSVAGKDDQNLYLSADLNLKRLKLGLNIRQSLTDQDERRIIMRLETWI"
     gene            10012..10863
                     /locus_tag="PLO35_02525"
     CDS             10012..10863
                     /locus_tag="PLO35_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH78568.1"
                     /translation="MKEIDFKLDECLIRLARLEDEPDLKAIAKTIWEGGDYLPRVMRE
                     WILEPWFIVCEYQGRVIACIKLTMLPDKSLWFEGMRVHGNLQGRGIGKLMNRAAMEYA
                     SRLASEHPGLRYEFSTYFLNHETLHLTSRMGFQQVESFHTLDKYGVRHLSEPDMITDP
                     GMDIFDHYPHYLPVGWRTVRKHPDGLAYIRSHATVFATPRARYLIGGHENKDITILDP
                     IPNDFRTELPYLQYFFGPKRKYSIIFPRSQASRIASLKNLGFRDWDDNPDRKPEMLVF
                     SMPAVSE"
     gene            complement(10872..11702)
                     /locus_tag="PLO35_02530"
     CDS             complement(10872..11702)
                     /locus_tag="PLO35_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78569.1"
                     /translation="MKKAALILITLCLCCAFLSAAGSGTQIPIDKVKKTLASIDPDWV
                     LISWLESETPNYWSLILREKDKDAIGTERILSLYQADTENGKRWVLHGLYADEYFWVF
                     LDEAYISQRREKETESIRTRLENMCQIVRGDFIMPFSMQGANRILEQVTRESVAANLN
                     FSLEAYPPDEALADTSYVELMADNSDWIDQYEMDFMEKYGYSNEQHRMFLAESEATYY
                     IHSVIGRTLVLMAIGNIAHKGEYVKLVCYLDLVTGTHRITTHSSKEKGESGDWSLPKD
                     "
     gene            complement(11711..12685)
                     /locus_tag="PLO35_02535"
     CDS             complement(11711..12685)
                     /locus_tag="PLO35_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78570.1"
                     /translation="MPGSKVIPSWIHFILLTLLLVWGVRIKDWFLDANRDYKSQVPNS
                     AKTLLLGDSHTVKLMDPSAWENAVNLGMSGEFIYYSYYKLNRLLKDGCRPHSVIISLS
                     YHSLATFPYFGGAEMNKRYHLLVDRRFYHRKFRYEDLHQSTLARYLGNAIGFPILVGQ
                     DIAEYYKLRNDSGTAGWMGAYEPATGSRIGKRRSLKAVLRRHFSKFGLPTGLSQAKLE
                     YLNDLARLCQIHGIELWLVNTPVHPDYYKGIPASFIKATDHAASSLGQYDAHYLNLST
                     EPYKDEEFYDYDHLNAEGSERFSKKLAQLTGGQQPGELGTTKTNNTLK"
     gene            complement(12672..13760)
                     /locus_tag="PLO35_02540"
     CDS             complement(12672..13760)
                     /locus_tag="PLO35_02540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOH78571.1"
                     /translation="MEVSGPDQWHPEVCLQRFPDFGAKLVVPLGISYLVFKYISYLTD
                     IYWSLIKSAKPLELLCFGSLFTIFSAGPLERFERFGKQIRREPLRPDFKLLEAGLIRI
                     CEGLFLKVVLADWLAYLIKPLWESPSAYGLQMRVLALFGYSLRIYFDFAGYSSIAIGA
                     SLLFGLKVSENFNSPYLSANITDFWKRWHISLSEWIRDYLFFPLSQGQSRRFWLQWMV
                     PVLAMLICGLWHGAAWNFALWGLWHGVGIAVYQMLQRWRKHRKLVTPGQVHWLRNLMG
                     MTITFSFVTLGWLAFHLDGLELALQLLSPLTLLYIPAMALILILLLGGFRRISDFALS
                     SRWRAIYALTLSLMFYCGMETGFIYARF"
     gene            complement(13643..14020)
                     /locus_tag="PLO35_02545"
     CDS             complement(13643..14020)
                     /locus_tag="PLO35_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78572.1"
                     /translation="MFTTLTLILVLGITAAVYWLIRSQHWRVIWLSLASLLWIWYWQK
                     EALHAVLALTLVTWLAGLWLERGSRKLRAYRLGVLFLVLALIAWKYLGLISGTLKSVY
                     SGSRILAPSLSFPLGFHIWSLNI"
     gene            complement(14088..14270)
                     /locus_tag="PLO35_02550"
     CDS             complement(14088..14270)
                     /locus_tag="PLO35_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78573.1"
                     /translation="MEEKLITFIRHEFLEDPDMELDANTKLISSGLVDSFSLVSLQAY
                     IKKRVWQDDTSSPDYR"
     gene            complement(14320..14754)
                     /locus_tag="PLO35_02555"
     CDS             complement(14320..14754)
                     /locus_tag="PLO35_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HOH78574.1"
                     /translation="MQWHELFPKDKPPSDDDINRFLGDRTARLWGDLIDACRQRWKAK
                     SRLTYSICSGKPGWNLKLQKSGKTLGTLYPERGAFSVFVVIPSGYEPKMRELLPDLSA
                     ELAELYNSAGDYMKVGKWMMFRIASRETMLDYLKILAIKMGP"
     gene            complement(14869..16374)
                     /locus_tag="PLO35_02560"
     CDS             complement(14869..16374)
                     /locus_tag="PLO35_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOH78575.1"
                     /translation="MKRFVFLCYLLLMAWSIRAVPVRIFHTNDTHGAYEPRNYRTPEG
                     NLALGGYLSLWEHLDSLRAEAPRSIYLDAGDQQTGTAFAAMPYAGAVGGAVIQVFNRL
                     GLDASTYGNHEFDHSPANLARLRSLARYPFVSSNLFDTTSSQPLGNKPWHVITRDSLR
                     IGILGLTLVELPEKVKIDNVSGLRILPYKEAIGMFLEDLDRQTDLIVVLSHNGFEADS
                     LLATQLDNRVDLIIGGHSHSYIESPTRVNGIYILQAGSYLSHLGLVDLEVENDRVTAF
                     DSRLIQLRQVPISRANPLALFVRQMSDRIEAQLGQVIASLPEDWVPDKFSSTALSRWM
                     ANSLKAQYQEIYQPDLAIVNNGGFRKLISAGPVTLRDMHEMLPFNNTVTVFSCYGRDI
                     LSWDALNAQIALAKPYDICQSTAPGWAATMHKDPNRPGETDWDGYTDRFFDLGGQRLD
                     PDQVYRVISHDYVAGQWDKYLGFKPFDVYDTGELFLDAMVREVIKQYGIKP"
     gene            complement(16398..17123)
                     /gene="kdsB"
                     /locus_tag="PLO35_02565"
     CDS             complement(16398..17123)
                     /gene="kdsB"
                     /locus_tag="PLO35_02565"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOH78576.1"
                     /translation="MKAVAIIPARIGSSRFPRKPLSTLGGKLVINRVWESVQASGLFE
                     QVIVATDSEEILNAVIVAGGRAQMTRPDHPSGSDRVAEVAQGLDVDIIINVQGDEPFI
                     DGEALRNLLNCFDDPAVEMASLMTPCLDPLVLRDPNIVKVITDLRGNALYFSRSPIPY
                     DRDGTSDYKTMRHIGVYAWRKRTLLRFVELSPSPLEHCEKLEQLRALENAIPIRMVET
                     AYQGVGIDTPGDLINAERLLCQA"
     gene            complement(17120..17926)
                     /locus_tag="PLO35_02570"
     CDS             complement(17120..17926)
                     /locus_tag="PLO35_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78577.1"
                     /translation="MKKTIILLCVVLLIGACSSHKAKKPLPASPPETEYLGFNAPAWL
                     WQIPSGSYAVGFGFTDTFYSNRADSLAREFAAVSLSRNHSAFVVDKQAVYNWASASQS
                     DWSSAGIKVVVSADMDYLKRANRTLKVIDAVDLQGYRIGLFGFIDGSVDNSTKPMRIE
                     ELPPWCVDESTELKGSTVICVASAVAASLPDAWNLAQEKALSLIGKYRIQKVRALFDS
                     TDDIGQRRLAVETVTKNYKAYLDKSFIVPQRKEKLISYRVYIQLKSSDVQ"
     gene            complement(17923..19857)
                     /gene="mnmG"
                     /locus_tag="PLO35_02575"
     CDS             complement(17923..19857)
                     /gene="mnmG"
                     /locus_tag="PLO35_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOH78578.1"
                     /translation="MTADYDVLVVGAGHAGIEAALASARRGARTAIFVIKLESVGRMS
                     CNPSMGGPAKGHLAREIDALGGELGYSADRSGIHFRMLNRKKGPAVWAPRAQNDRRLY
                     SELMLRALEEQDGLDIIESTVCEIMVKDSAVTGLRTSIGLEYHAPAVILATGTFLRGS
                     IHVGTSISEGGRSGEPAANELSNSLEKLGFNLIRFKTGTPPRVDLRTLDYSVLEEQPG
                     DPDPSGFSYYRDVVLKNQVSCFLTRTTPETHNIISANLQLSALYGGIIKGIGPRYCPS
                     IEDKIVKFPQRDSHQIFIEPEGLNTWEGYVNGISTSLPPEIQARIVRSIPGLEQARIM
                     RYAYAIEYDCVPADEIDASLKSKRIKGLYLAGQINGTSGYEEAAAQGMLAGINSTLWL
                     EGRDSLILGRDQAYMGVLVDDLVTRGTNEPYRMFTSRAEYRLLLRQDNADERLMRIGH
                     ELGLVSEIRWQRFLEMLRIKERELERLKTEKSLFCRSVLRNEAGQESYNSEQVPELPK
                     EPTRFAILLKRPEIAFEDLRRFGYQPSAELTSDIAGRIELEIKYEGYLDRASAELERF
                     NSSEQISLPENLDYHAIHSVAWEAREKLARIRPRSIGQAMRIPGVNYSDAAALLIWLR
                     KHHIHKESGERELASPKESM"
     gene            complement(19866..20477)
                     /gene="yihA"
                     /locus_tag="PLO35_02580"
     CDS             complement(19866..20477)
                     /gene="yihA"
                     /locus_tag="PLO35_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOH78579.1"
                     /translation="MFRLVQSYFVKSAVEPVDYPASAFAEFAFAGRSNVGKSTLINLI
                     TGVHGLAKTSSTPGKTRLLNFFLVRWKDEETDSEGKLQLTDLPGYGFAEVGQAEQEKW
                     RKMINRYFEVREQLKAIIVLVDIRHPADDKDILMLELLRLRGIDHCVVATKADKIPKT
                     KIPKTLQLLAKGLGLKTEPIFPVSALKKTGMEQVYGWMRGRLI"
     gene            complement(20470..20841)
                     /gene="queD"
                     /locus_tag="PLO35_02585"
     CDS             complement(20470..20841)
                     /gene="queD"
                     /locus_tag="PLO35_02585"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOH78580.1"
                     /translation="MYRLSVSDSFSAAHRLCGYQGACRNLHGHNWKVKVCLATDQLDE
                     IGMALDFGVIKQFLASILDELDHAYLNELPAFTQHNPTSENLARYVFERFERELAGKP
                     VQVESVEIQESERSAVVYSHV"
     gene            complement(20851..21783)
                     /locus_tag="PLO35_02590"
     CDS             complement(20851..21783)
                     /locus_tag="PLO35_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOH78581.1"
                     /translation="MIQYFKIAEGRFVPAVTHHEAFWIHLDSPNGDEIKTLIERYDLP
                     EDFITDLQDSDENSRVEYEDGATLIIIRVPIYYRHRSASLSFMTAPLGIIAVQDKLIT
                     VSFYDSEILTQYLEAKHRPFLITQQSFLLQINLRAAIYYLKFLKEINRRTNNIENELH
                     QSMRNKELIRLLRMEKSLVYFSTSLRSNEIILERLQRSRWLNQDPEAEDMIEDVIIEN
                     KQAIEMANIHSSILSGMMDAFASIISNNLNVVMKFLTSITIIISLPTLIASIYGMNVH
                     LPFQENAYAFAIVMGISVLVSISLVLIFIRKKYF"
     gene            22031..22432
                     /locus_tag="PLO35_02595"
     CDS             22031..22432
                     /locus_tag="PLO35_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78582.1"
                     /translation="MAFHVNFHTSFDLSQAVADDGFSLGYEASWYWAVLPVNEELDSP
                     DPDSDEFMIWRFSTMADPMGELEVPNVSIERLDNSVRVWWPAVPNASTYKVYGSVDPI
                     LGYSLITQTADTEWIVTTPGNMEFFKVAAVN"
BASE COUNT         5156 a         6074 c         6130 g         5140 t
ORIGIN      
        1 tgggcaagct actgggcaag ctctttacgc caagaagcaa gagactgcgc gtttgataga
       61 agaactaaca caagtgcaaa taggcataat cagaacttgc acagaacctg ttagcttgca
      121 ggaaatcaag aaaagccaaa atcttgtgag cagagacttt ctgagagtaa gacatttaaa
      181 tccgctgctt gaagatggta tcgttctgat gaaatacccg gataaaccta agcaccctaa
      241 gcaaaagtat tatcttagcg actatggaaa agaagtgtta caagccattc caaaagagca
      301 ttgatgctac agccaagcgc tattgatctt gagccccaag ccaagggtcc agtcacctgc
      361 ctggcacagg aatttgccat cgaagctttc cccataataa cggatttatg ttgacagctt
      421 tatcgggata ttcaaacatg caaacaatgt aatcttttta ctctttactg gaggaaccgt
      481 gaaacaggaa tccctagtaa cgggtcaaag ccatccaatt agcctccttt acaccaccta
      541 tgcctctcct ttgcctctcc catgcttagg cataggtgga gtatgggaga tgcctgagat
      601 atgaaaagca ggctttgctg gaatagagag atgcaggttt gtggaagcgg aaacgtcctc
      661 gtttcagtgg cctgtaacgg atgacggata atagttaaat ccaacaaagc tcagagaaag
      721 cagaggattt atcatcgcac acttaaatcg tgtaaatact gtcatcccgt cctcagggtt
      781 ctgtgaaaat cagtgtcaat cagtttcatc agtatcatct gcgttctctt attctacgcg
      841 gttcacgtag tcggatgaga cacctgctga gccctgcaga tttggattga caaaatagct
      901 tgctccggcc aagatgcaaa gacaaagaat ctggagtgtc tgatgagttc actggccgtt
      961 ttgggatgca tgtggggcga cgaagccaag gccaagatag tagattttct gggtgtctcg
     1021 gctgacgtgg tggtccgctt tcagggtggg gccaacgcgg gtcacaccat cgtggtggag
     1081 ggccgtaaat acgtgtttca cagtgtgcca tccggcattt tgtatccaaa gtgcaagtgc
     1141 gtgatcggtt cgggagtggt ggtggacccc tttgccctca gggaagaaat tctggcccta
     1201 agggaagcgg ggatcagttt tgagcacagg ctgttcatcg atgaccgggc cgcgatggtg
     1261 cttcctctgc accagcgcct ggatttgatg caggagaaaa gcctgggtaa atccaggatc
     1321 ggtaccaccg gtcgggggat tggccccgcc tattccgaca gagtttcccg ccgggcgtta
     1381 agactggcgg acctgctctg gccggactgg ctaaagcagc gtctgtcagc tctttacgcg
     1441 catcatgggc tcaaagtgag caaggcggag ctaaaaggcg aagttcaggt cctccaggag
     1501 ctctatctct ttctcaaacc ctatcttgct cagaccgaca gcctgctctg ggattggcat
     1561 ctgcaagatc aatccatctt gtttgaagga gcacagggta gcctgctgga tctcgtgtat
     1621 ggcagctatc cctttgtgac gtcttcacat accttgagcg gagggatcag tgtgggcact
     1681 gggctgccgc cccgctcgct cgataagatc attggagtct ataaagctta cagcaccaga
     1741 gtgggcgaag ggccttttcc cacggagctg tgtgatgaac tgggagacag aatcagggtg
     1801 cagggcaatg agtttggctc caccaccgga cggcccagac gggtgggatg gtttgatgcc
     1861 atggcggcca ggttcacggc ccgcctgaat gggatcgaca gcatcgccct taccctgctg
     1921 gatgtgctga gcgggatcga agaactgcgg gtctgcacag gctattggat tggcggccag
     1981 cggcttgagc ggtttccctc gcatcatctg gacctcgaac aagtccagcc tgaatacctc
     2041 agcctgccgg gttgggacaa ggatatcagc cactgccgca gcctgagcaa attaccccgg
     2101 gccgccaagg attacctgga agccatccag gacctgatcg agaaacccat cgagctggtc
     2161 tcggtgggca aagaacgcaa acaaaccatt gtgataaaat gatccggaga tacaatgaaa
     2221 cactttagcc tcttgatcct gctgctgata gcttcggccc tcagcgccca ggctgcagac
     2281 agcctgaact tttccctgcc gcaaaacatg gaccccgtgt ttgagtatta cagccgcaac
     2341 tacctttcca cccgttccat gggtcgtggg accacgggtg tggcctccct gggaggagtg
     2401 gaatcggtgc ttgccaatcc cgcctcttat cagatcgatc gcgacgccat gtatatggaa
     2461 ttgctgatca aacctcccgt cagcacagct atctataccg gagacaacaa cttcaagtct
     2521 cccgtcccct ttgggctggt ggccgcgggg ggaaacgtga cggacgatct cacctggggc
     2581 gcggcttaca gcctgccaaa atcgctggtg atggatgcct tcaacctgga aatgaacctg
     2641 ggcggatacc tgcacacccg ctatccggtc tacaacctgc accagttgac ggccaacctc
     2701 ggtctgcaca tcggagagct caatctgggc ctcaacgtcc acaaccagtt tcactatatc
     2761 agcgatttta ccgtgctgag gaccttcgag cggatccgcg aggtcaaata cttgctgcga
     2821 cctcagatgg ggatgctctg gaacacccaa aagctcaatc tgggctttag ctacactccc
     2881 caaagtcctt ttaactggga cctcaagtat gtgatgtatg agacctctct gcctggggag
     2941 gcgagcgtcg gtctcagcgt ccgcgatgcc aaccgccgtt acaatctgga aatggattgg
     3001 accgacacct cgatcctcaa ctcccattac aaggaccgct atacctacaa agccgggttc
     3061 gaggccgatg tgcgaaaatt cacctaccgg cttggttatc tgtatcgtcc tcagatctgg
     3121 gacgggctct accagctgcc tgtcaacacc acagccaatg ccgatacagc tctctggtgg
     3181 gacgacgctg ctctgggagg cagagtgcca gccaacgatc aacacctggc cacggctggc
     3241 ctgagctggt ttttcaaaga cggctgcatc aatctgggtt tgctgtatga agtgtctggc
     3301 cccgcgcaga tggctcaggt gaacgcgtcg ctggggctca actttgatgt ctttaaacgt
     3361 agaaagcatc cggcggagaa atgaaggcca gagtccagat ccgcaaactg gaaggttatg
     3421 agccggagcc cttgcgggaa gccgtagcca gctttctgaa gaccgtccgc aatcccaaga
     3481 tgagcaaggc caagagggtg ctgatcaagc ccaatctgtt gggtgcccac gctcccgaaa
     3541 aggccgtcac tactcatccc atggtggttg aggccataat ccggcatttt ttggataaag
     3601 gcaaagaagt gtgggtggga gacagccccg gtggcactgt accggtggaa acggtatggc
     3661 agacgtgtgg ttatgctgac cttgccaagc gctatcccat caaactggtc aatctctcca
     3721 ccctgggttt caaggaactg cggcatgggg aaaacacggt gaagatttcg gaggcatttt
     3781 ggcagtgcgg gattgtgatc aacgtagcca aatacaagac ccacagtctg atggcctaca
     3841 ccggcgcgct caagaacctc tatggtctgg tcccggggct ggtcaagagc gattatcatc
     3901 gccaacaccc tgataccaat agctttgccc aattgctgat cgacctctgg gcgctcacca
     3961 gaagccgggt cacctatagc tttatcgatg ggattctggg tatggacggt gccggtccgg
     4021 cggctggcag gccacgcaat ttcgggcttt tcttcggttc cgattcgatc ccggccctgg
     4081 attttgtcgc ctcccggatg atgggattcc agctcaagga catcccctat ctcagcgagg
     4141 ccctgcagat ggatgggata ctgccatccc ggatcagcat tccggcaagt ttcagagatt
     4201 atccctccct caatgccgaa ctgggagtgg tgaaactgcg caaagacctg ctcaaatatg
     4261 tgccgtcctt tgcccgcaaa ggttttcaga aggtctatta catccatccc gtgatcgggc
     4321 cggactgcca gcgctgcgga atctgcgtta agagctgccc cgttaaagcg atttcctggc
     4381 agccggacgg ctttccgctc atcgaccagc agacctgcat caagtgcatg tgctgtcatg
     4441 agttgtgtcc tcatcacgcg gtagagatca accggtctct tttggccagg atggtgcagc
     4501 agtgaaaccg cgctcaggca agcccaatcc tttgctgcgc ctggtctttt acgcggcaat
     4561 gctgtttgtg gtctcctgga ttctgctctg gggcgagcac agttttttgc gcacctggaa
     4621 catggggcgc aaagtgcagg ccctccaaaa ggaaatgcaa gaactgcaga cgattaacga
     4681 agaactgaag ctggaaaacc agcgcctcaa atccgatccc agggcggcgg aaaaggtcgc
     4741 tcgcgagaag tttggcctca ccaaggaagg ggagaaggtg tttcgcttca tacccagcgg
     4801 gccagcggcc aagtcagaag cgaaagcaga ttcgcaatga cagccatcca ggacgttcct
     4861 accctgagtt ccgtgctccc ggaacagcag cagcggatca gttcgctaat gggcaaactg
     4921 ctctatgata cagaccagga acgactgaaa cacatttcgg aactggtgga tatgatcgat
     4981 gccgaagaac gggaaggtaa ccacggacag gccttcagag agtattcagc cctttttatg
     5041 gacagcctga tcactctgct cacggggggc aagagctcgc atcgctctac cctgtttgag
     5101 tttatggacc tgctgaactt tttgccgatc agccccaagc acagacgcta cactttgctg
     5161 aaagcagcgc ttgatcccta tctggccgaa taccccgacg tgtatgagcg gttcgaccaa
     5221 tatcaggctt tgctgctcct gacccgtctg ggagactatg aaaacggagc gcggctgctg
     5281 gatgaactgg ccgacggagt ggatatatcc aggcctcagg tttatctgat ctatcatctg
     5341 gctaaagccc gcgtgctcta tcagcaggag aggtatctgg aatttgtggg gcactggctg
     5401 gacctgattc ccaggtttta tcagacggac agcgcggatt tggccgtcta tctcttaatc
     5461 gtctggagcc ggaccatgaa ctggcaaaat gattcgatgc ttcttaaggc cttgctggaa
     5521 aaaatctccc gggggctcag ccatcagcag aatctgaata tggctatggc aacctacgat
     5581 cgtttttgcc tcgaaaacag gctggtgggc cctgatgata agatacagct ggcagaacgg
     5641 ctgatcaagc gcttttcctc gcatctctct tcccgtcagc tgcaagagct gcatttcttc
     5701 gcggggaact actcatccgg gatgcgttcc agctttctgg acagcatcca gtacttcaag
     5761 tactcaaatt actatctgaa caggtgttgg aacgaacaga cggaaggggc ccgctttctg
     5821 agggggatcc tctcaccgga gcatttttgt atggcgatgc cctatatcga aaaacgcatc
     5881 caggtgatgg gcaatcaggt gagcatgcac aacaactcct acgtggaatc gctgcaggct
     5941 gactactcca agatcgaatc gctgttgcag caggtggagg acctctccat cacagacagc
     6001 ctcaccggcc tcagaaaccg cagatttctg gaagtcaaca tctatcagct cctgctgctg
     6061 gccactcgtc aaaaagcccc catcagtttt gccatgctgg atatcgatca ctttaaaaag
     6121 gtcaacgaca cctggggtca ccaggcaggc gatcaggtgc tcaaggagct ggcctgcatc
     6181 ctgtctgagg agttccgcaa gagtgacgtc atcatccgct acggcggcga ggagtttctg
     6241 atggtcctct tcgattcccc gctggatgcc actctggtca agatggaaat cctacgcgcc
     6301 aaggtggaaa cccatccctt cacatacaag ggacgcagga ttcacgtcac ggtgagtata
     6361 ggcatcaacc gcaacaacat agttatttat aacgagcagg atatccgcac caatatagat
     6421 atcgcggatg cagcgctgta taaggccaaa aacgagggcc gcaatcaggt gagggtgatg
     6481 tgacacgctg gcgcgcaacg gcttatatcc gcaaagcggc tccctcacag caaaggccga
     6541 ttgcccgtca cgcattttcc cgcttgacat tatctcccta tccaaaaatg taaagcgcaa
     6601 aggagtttta catggcaaag atcacggtaa agaacctgaa caagtattac gacaacggtt
     6661 tccacgcagt caaagatgtg tgctttgaag cggaagacca ggaattcatg gtgttggtgg
     6721 ggccatccgg atgcggaaag accaccgtcc tgagattgat cgccgggctc gaagagattt
     6781 cttcgggtga gatctggatc ggtgacaagc tggtcaacaa ggtccatccc aaggaccgcg
     6841 acatcgccat ggtgtttcag aattacgcgc tgtaccctca tatgaccgtc aacgaaaaca
     6901 tggctttcgc cctcaaactc cggggagatg ataaagccac catcaaaaag aaagtggacc
     6961 acgccgcctc ccttttgggg attgaatcca tgctcaaacg ccgtcccgga cagctttccg
     7021 gaggtcagcg ccagagagtg gctctcggga gggcgattgt gcgcaacccc aaggtctttc
     7081 tctttgacga gcccctctca aacctcgatg ccaagctcag agtgcagatg cgctccgaga
     7141 tcgtgaagct gcaccgccaa ctggaaaaca ccatgatcta tgtgacccac gaccaggtgg
     7201 aagctatgac catggcggac cgcatcgtcg tgatgaaaga tggcgttatc catcagatcg
     7261 atactcccct caatatctat aaccggcctg ccaacgtgtt tgtggctggc tttatcggga
     7321 gcccggccat caatctggtc agcggtaagc tggcaaacct ggatggcggc ctgggatttg
     7381 tgagtgccgg cctcaagatc aagctcaccc cccaacaggc gcaaacgctg aatgcttacg
     7441 ccaacaaagc catcaccttt ggcatccggc ctgaggatat ctacgacgcc cgctatgaca
     7501 gcatggcgga gcaccctcag gggatagaca cagtttgtga tattgtagag cccctcggca
     7561 acgagtatca tctgcagctc aaagccggag agcatgatct caccgctcgc ttcgatccca
     7621 aagaactgcc taaagcagga cagacgctga aggtgagttt ggatatgggc aaagcccatt
     7681 tcttcgatcc cgaatccgag cttagcctga tttgaagctg aggaggccgg tcatgcgcct
     7741 cactatcatt attatgttgg cactttgctg tggctgggtg gccgcgcacg ccgacctgag
     7801 cgaggaaacc gcctcggcga acgctctgtc tggcgtgacg gcactatctt tcagtcccgc
     7861 ggaccggatc ctgtctcccc tggtgttggg cagcggaact gtcttcacct atcatttgcc
     7921 ctttggagac ggcgataccc aggtgtgggg cttccacaac ggcagccggc tgagcatcgg
     7981 acacagcggt tttggtgtaa gctggctgag ccatcccgac tacctctggc gggaccacta
     8041 tctcagctac gcgctgggat cccgggattt tgccctgggc tatacccagc acctgctcta
     8101 cgagagtttt tcctcgggag aatcatacta cacttggagc ggagatctgg ggctgggctt
     8161 ttatggcggt gattacggct cggaagtgag atggctgcgc atcggttctg aagacgccca
     8221 atggcatttc acggccatca cctacctgag cgaatactcg tccatcgcca cagattatgt
     8281 ctatcaggag catggcaagg acagcgtgag ggcagcctct tctatcggga tatcagacct
     8341 gtttgccatc cagacatcct ggcaaagcga ccctccccgc tttggctttg gcacccgtct
     8401 caagctcagc tacggcacct ttatgtatgc cgttcgcact catccagaaa tgggcctcag
     8461 ccacagcgtg gacgtcggtt ttacctggtg agaatgctct tcctggcgct gctgttgctg
     8521 ccgctggccc ttgtggccca aagcgaagcg gaattgatcg agctaaacgc tgaaacgctg
     8581 gaattcaacg cctggcagga actttacaga caattgctga gcccgggttc ggaactgggc
     8641 ctggacaacc ggctgtggct gacggaccag agctatagcc tccaccggct gatgtttcgc
     8701 caccagggca gagaactggc cctcaatctt tacaatgact ggactgatgg cgataccgat
     8761 gccaatgtga gcatccggct ggcccaggac aaactgttga gagatgtagc gctgggccat
     8821 tatcgcctcc agtttggcac cgggatatct atgggcagcg gctcacgaag tctgaagggt
     8881 ccggtactcc agatcgaacg gccaccctca cccaggcgtt acagtccctt tggggccgcg
     8941 atcaaaactc aatggaaaca agtggccggc ttgctgtttg ccagtgtggc agcacgcgag
     9001 gccaggctca acgcggccag cgagatcatc agcctgccca ggaccaaaac cgggctcgac
     9061 ggtagcaccc gggaaaacct ctggggagcg gccctgagct atgaggccaa gcttttcagg
     9121 ctgggcgtta tcgcatatcg ccaaagctat gacagggact tcgaaggagg ccttgagcgg
     9181 gtttccacgg ctggaagcgt ttatggcgcg ctgcggttca aagctcatac cctggatctg
     9241 gaaagcggcc tgaccaatga ctccccccac cattatctgg cctggaatta ccggcatcag
     9301 ggctttgaac agaccctcag ttttgccatc gatccggatc aggatcaagt ggcttattcc
     9361 actccccggg aagtgcttag ccgcgatccc gacaccatgg agatggcctg ggacgccaga
     9421 tttccccttt tcaaagatgc ccggctgggg ctccgttttg ccgctgacca tctcaggggt
     9481 ggcagcgtca gcaccaatga tctgcgcacc agactcctgg cctcttttga atacgggcag
     9541 caagctgaga acttcagctt cagagtgagt catttcgacc gggaagtatt gagccacata
     9601 gacgaggact acaacctaag caggccaaaa cactggcgct ttgaactggc ttacaaagat
     9661 caggtgctgc cttacctggg cctgatgctg aactgccgct atcacataga ggataaaagc
     9721 agttggaaag acaatgggct ttactatcac agcgctctgc agtttcaaag cggaaagctc
     9781 agcgcccggc tgggctatca gggctggcaa agctccaaaa ccggtctctg gtatgaggac
     9841 gacaccctta gcgcctggtc cgttgccggc aaggacgatc aaaacctcta cctgagcgct
     9901 gatctgaacc tcaaacgcct caagctgggg ctcaatatcc gtcagtccct cacagatcag
     9961 gacgagcggc ggatcatcat gcgcctggaa acctggattt aagagaaata aatgaaagag
    10021 attgacttca aactggatga atgcctgatc cgtctggcta gactggaaga cgaacccgac
    10081 ctcaaagcca tcgccaaaac catttgggaa ggcggggatt atcttcccag ggtgatgcgg
    10141 gaatggatcc tggaaccctg gtttatcgta tgtgagtatc agggcagagt gatcgcctgt
    10201 atcaagctta cgatgctgcc ggacaaaagc ttatggtttg aaggtatgag agtgcatggc
    10261 aatctgcagg gacgcgggat cggtaaactg atgaaccgcg ctgccatgga gtatgccagc
    10321 cgtctggctt ccgaacatcc cggcctgaga tatgaattca gcacctattt cctcaatcat
    10381 gagacgctgc atcttacttc caggatgggc tttcagcagg tggaaagctt tcacaccctg
    10441 gataaatacg gggttcgcca tctcagcgaa ccggacatga tcacggaccc gggcatggat
    10501 atctttgatc actatccaca ctatctgcca gtggggtgga ggaccgtcag aaagcatccc
    10561 gacggccttg cctacatccg ttcccatgcc accgtgtttg ccacacccag ggcgcgatac
    10621 ctgatcgggg gccatgagaa caaggatatc acaatcctgg accctatccc gaatgacttc
    10681 aggacagaac tgccttacct gcaatacttt ttcggcccca aacgcaagta ttccatcatc
    10741 ttcccgcgat ctcaggcttc caggatcgcc agcctcaaga acctgggctt ccgggattgg
    10801 gacgataatc ccgacaggaa accggaaatg ctggtgttca gcatgccggc tgtgtcagag
    10861 tgatcacaag ctcagtcttt tggcagactc cagtctccgg attcgccttt ttctttgctg
    10921 ctgtgagtgg tgatcctgtg ggtgccggtc accaggtcca gatagcagac caatttgaca
    10981 tactcgcctt tatgcgctat gttgccaatc gccatcagga ccaaagtgcg gccgattacg
    11041 ctgtggatat agtaggtggc ctcactctct gctaagaaca tgcgatgctg ttcgtttgag
    11101 taaccgtatt tctccatgaa atccatttca tactggtcga tccagtcact gttgtcggcc
    11161 atcagttcca catagctggt gtcggccagt gcttcatccg gaggataggc ttccaggctg
    11221 aagttcaggt ttgcagccac agattcccgc gtcacttgct ccaatatccg gttcgcgcct
    11281 tgcatgctga agggcatgat aaagtcccct ctgactatct ggcacatgtt ttccagcctg
    11341 gtcctgatgc tctctgtctc cttttcacgg cgctgcgaga tataggcttc atccagaaac
    11401 acccagaagt attcgtctgc atacaaccca tgcaaaaccc agcgtttgcc gttttctgtg
    11461 tctgcctggt aaagactgag aatccgttcc gtgccgatag cgtccttgtc cttttctctc
    11521 aggatcagac tccagtaatt gggagtctca gactccagcc aggagatcag cacccaatcg
    11581 ggatcgatgg acgcgagggt cttttttacc ttgtcgatag ggatttgtgt gccgctgccg
    11641 gccgcgctca ggaaagcaca gcacaggcac agcgtgatca gaatcaaagc agcttttttc
    11701 atggcttacc tcacttgagt gtgttatttg tcttagtagt tccaagttct ccgggctgct
    11761 ggccccctgt caattgggcc agcttcttgc taaagcgttc tgagccctcc gcattcaggt
    11821 ggtcataatc ataaaactct tcatctttat agggctcagt gctcagattc agatagtggg
    11881 catcatactg gcccaggctt gaggcggcat ggtctgtagc cttgatgaat gaggcaggga
    11941 tccccttgta gtaatctggg tggacgggtg tgttgaccag ccagagctct atcccatgta
    12001 tctggcaaag cctggcaagg tcattcagat actcaagttt ggcttgggaa agtcctgtgg
    12061 gcagtccaaa cttgctgaag tgtctacgca atacggcttt gagagagcga cgtttcccaa
    12121 tcctggagcc tgtagctggc tcataagctc ccatccagcc tgcagttcca ctatcgtttc
    12181 gcagcttata atactccgcg atatcctgtc ccaccaggat tgggaagcca attgcgttgc
    12241 ccagatatct ggccagggtg ctttgatgta aatcttcata gcgaaacttg cgatgataga
    12301 atcggcgatc caccagcaag tgatatcgct tgttcatttc agctccgcca aagtatggaa
    12361 aagtagccag agagtgatat gacaggctga ttattacgct atgaggccgg cagccgtctt
    12421 ttagcaagcg gttgagtttg tagtagctgt aatagataaa ctcaccactc attcccagat
    12481 ttaccgcatt ttcccaggcc gatgggtcca ttagtttgac ggtgtgagag tctcccaata
    12541 atagtgtctt agcgctgttt ggaacctggg atttataatc ccggttcgca tccagaaacc
    12601 agtccttgat cctcactccc cataccagta acagggtcaa cagaatgaag tggatccagc
    12661 tgggaataac tttagaacct ggcatagata aaacccgtct ccattccaca atagaacatg
    12721 aggctgagag taagagcgta gatagctcgc catctggagc tcagagcaaa atcactgatg
    12781 cgcctgaaac ccccaagcaa caaaatcagg atcagggcca tagctgggat atacaacaag
    12841 gtaagcggag acaggagttg gagcgccagc tccaggccat ccagatgaaa ggccagccag
    12901 cccagagtca cgaaactgaa agtgatcgtc atgcccatga ggtttcgcag ccagtgtact
    12961 tgcccggggg ttaccagttt acgatgcttg cgccagcgct gcaacatctg atagacggct
    13021 atcccgacac cgtgccagag gccccacagc gcaaaattcc aggcggcacc atgccagaga
    13081 ccacaaatca gcatcgccag aactggcacc atccactgca gccaaaaacg ccgtgactgt
    13141 ccctgcgaca acggaaaaaa gagataatcc ctgatccact cagaaagcga gatgtgccaa
    13201 cgcttccaga aatcagtgat gttcgcgctc agataagggc tgttgaaatt ctcactgacc
    13261 ttgaggccaa acagcagcga tgccccaatg gcgatgctgg aatagccggc aaaatcgaag
    13321 taaattctca agctataacc aaataaggcc agcaccctca tctgcaggcc ataagcggac
    13381 gggctttccc acagcggttt tatcaggtat gccagccaat ctgccaatac aacttttaaa
    13441 aacaagccct cgcatattct gatcaatcct gcctcaagca acttgaagtc tggtctaaga
    13501 ggttctcgtc tgatctgctt cccaaagcgt tcaaagcgct ccaggggtcc cgcgctgaag
    13561 atggtgaaca gagaaccgaa acaaagcaat tccagcggtt tggcgctctt gatcaggctc
    13621 cagtaaatgt ccgtaagata acttatatat ttaaagacca gatatgaaat cccaagggga
    13681 acgacaagct tggcgccaaa atccgggaac cgctgtaaac agacttcagg gtgccactga
    13741 tcaggcccag atacttccaa gcgatcaggg ccagcaccag gaacaggact cccaatctgt
    13801 aagccctgag ctttcgggaa ccccgctcca gccacaatcc agccagccag gtgaccagcg
    13861 tgagagccag caccgcgtgc agcgcttctt tttgccagta ccagatccac agcaggctgg
    13921 ccaggctgag ccagatcact ctccagtgct gggagcggat cagccaatag acagccgcgg
    13981 tgattcccag caccagaatg agcgtcaggg tagtgaacat cggtcggttt gactgctgtt
    14041 agaattgctc gatgatcttc accatttggg ctacggtatc aaagctctca gcggtaatcc
    14101 ggggagctgg tatcatcttg ccaaactctt tttttgatat aggcttgcag tgaaaccagc
    14161 gagaaggaat ccaccaatcc gctggagatc agcttggtgt tggcgtccag ttccatatcc
    14221 ggatcttcca gaaattcatg cctgatgaag gtgataagtt tctcttccat tatattgctc
    14281 cttataggtt tattgtatgc tgtaggggag gtgtgggcct cagggaccca tcttgatagc
    14341 caggattttc agatagtcca gcatggtctc gcggctggcg atcctgaaca tcatccactt
    14401 ccccactttc atgtagtcgc cagcggagtt gtagagctct gccagctccg cgctcaaatc
    14461 tggaagcagt tcccgcattt tgggttcgta tccagagggg atcaccacaa acaccgaaaa
    14521 cgctcctcgc tcgggataga gcgtacccag agtcttgccg ctcttttgca gcttgagatt
    14581 ccagcccggt ttgccggaac agatgctgta tgtgagcctg gatttggctt tccaccgctg
    14641 ccgacaggca tcgatcagat caccccaaag cctggcggtc cgatcgccca gaaagcggtt
    14701 gatatcatcg tctgacggtg gtttatcctt gggaaatagt tcgtgccact gcattagtca
    14761 aactcctctg cgtggtatga tacaatcgtg atgatagccg ttttgaggac gagtgctgtc
    14821 cagcgccaga gatcatggcc ggctactgac ttcccgagca ggtaaagctt atggtttaat
    14881 cccatactgc ttaatcactt ctctcaccat cgcgtccaga aagagctcgc cggtgtcgta
    14941 gacgtcgaat ggtttaaacc ccagatattt atcccactgt cccgccacat agtcatgtga
    15001 gattacccga tagacttgat ccggatcgag acgttggcca cccaggtcaa aaaagcggtc
    15061 ggtatagcca tcccagtccg tttcaccggg gcggttggga tccttgtgca tagtggcagc
    15121 ccagccggga gcggtgctct ggcagatgtc gtaaggtttg gccagcgcga tctgagcgtt
    15181 gagcgcgtcc caactgagga tatcccttcc atagcatgaa aagacggtaa cggtattgtt
    15241 gaagggcagc atttcatgca tatccctcag agtgacaggt ccggcggaaa tgagctttcg
    15301 aaagccgccg ttgttgacga tggccagatc gggctgatag atttcctgat attgggcctt
    15361 cagggaattt gccatccagc gcgaaagggc tgtggaagaa aacttgtccg gtacccagtc
    15421 ttccggaagg gatgcaatca cctggcccag ctgggcttca atgcgatctg acatttgcct
    15481 cacgaaaagc gcgaggggat tggccctgga gattgggacc tgccgaagct ggatcagacg
    15541 gctgtcgaat gctgtgacgc gatcgttttc cacttccaga tcaaccaggc caagatggct
    15601 taaataactg cccgcctgca ggatatagat accgttcacc cgcgtggggc tctctatata
    15661 gctgtgactg tgacctccga taatgagatc aacccggttg tccagctggg tagccagcag
    15721 gctgtctgcc tcgaagccgt tgtggctgag gaccacaatc aggtccgtct ggcggtccag
    15781 gtcctccaaa aacatcccga ttgcctcttt gtatggcaga atcctgaggc cgctcacgtt
    15841 gtcgatcttc accttttcgg gcagttccac caaggtgagc cccaggatgc cgattctcag
    15901 tgagtctctg gtgatgacgt gccatggctt gttacccaga ggctgggaac tggtggtgtc
    15961 aaacagattg ctgctcacga atggatagcg ggccagagaa cgcaagcggg ccagatttgc
    16021 ggggctgtgg tcaaactcgt gattaccgta ggtgctggca tccaggccca gacgattgaa
    16081 aacctggatc acggccccac ccacggctcc ggcataaggc atcgcggcaa aggcggtgcc
    16141 ggtttgctgg tctcctgcat ccagataaat gctgcggggc gcttcggctc tcagggagtc
    16201 aaggtgttcc cagaggctca gatagccgcc cagcgcgagg ttaccttccg gggtgcggta
    16261 gtttctgggc tcataggccc cgtgggtgtc gttggtgtgg aaaatcctca cagggactgc
    16321 ccggatggac catgccatca ggagcaggta gcagagaaag acgaagcgtt tcatgggctg
    16381 gcctcctgtt atctcgatca agcttggcag agcaggcgtt cggcgttgat cagatcgccc
    16441 ggagtgtcta ttcccactcc ttgataagct gtttccacca tgcggatggg aatagcgttt
    16501 tccagggccc tgagctgttc cagcttctcg caatgttcca gggggctggg gctcagctcc
    16561 acaaagcgca gcagagtccg cttgcgccag gcatatactc ctatgtgccg catggtcttg
    16621 taatctgaag ttccatcccg gtcgtagggg atgggggagc gggaaaagta gagcgcgttt
    16681 cctctgaggt cggtgatcac cttcacgatg ttggggtctc tcaatacgag aggatccagg
    16741 cagggcgtca tcagagaggc catctccacg gcggggtcat cgaagcagtt caacaggttc
    16801 ctgagggctt cgccatcgat aaaaggttcg tctccctgga cgttgatgat gatatcaaca
    16861 tccagtccct gagccacttc cgccacgcga tcgcttcccg atggatggtc cggccgggtc
    16921 atctgagctc gtcctcccgc cacaatcacg gcgttgagga tctcttcgct gtccgtggcc
    16981 acgatcacct gctcaaagag gccactggcc tgcacggatt cccagacgcg attgatcacc
    17041 agtttgccgc ccagcgtcga taagggcttg cggggaaagc gactgctgcc gatccgggct
    17101 ggaatgatcg cgacggcttt cattggacgt ccgagctctt caactgaata tagacgcggt
    17161 agctgatgag cttctccttg cgctgaggca cgatgaagct cttgtccaga taggctttat
    17221 agtttttggt aacggtctcg acggccagac ggcgctgacc gatgtcgtcg gtgctgtcaa
    17281 agagggcccg cactttttgg atgcggtatt tgccgatcag gctgagggcc ttttcctggg
    17341 ccagattcca ggcgtccgga agactggctg ccaccgctga agccacacaa atcacggtgc
    17401 tgcctttcag ctcggtggat tcatccacac accagggtgg caattcttcg attctcatgg
    17461 gtttggtgct gttgtccacg ctgccgtcga tgaagccaaa cagcccgatc cggtatccct
    17521 gcaagtccac ggcgtcgatc actttcagag tgcggttggc ccgtttaaga tagtccatat
    17581 ctgcgctcac cactactttg atccccgcgg agctccaatc tgactggctg gcgctggccc
    17641 agttgtaaac ggcctgttta tcaaccacaa aagcgctgtg atttcgggag agcgagacgg
    17701 cggcaaattc gcgggccaga gaatccgccc ggttggaata gaaagtatcg gtaaagccaa
    17761 accccaccgc gtagcttccg gaaggaatct gccacagcca ggctggagcg ttgaagccca
    17821 gatattccgt ttcgggaggt gaggccggaa ggggcttttt agctttatga gagctgcacg
    17881 cgccaatcag caggactaca cacagcaata ttatcgtttt cttcacatac tctccttggg
    17941 gctggccagc tcccgctcgc cagattcttt gtggatatga tgttttcgca gccagatcaa
    18001 cagggcggcc gcgtcgctgt aatttactcc agggattcgc atcgcctggc caatgctgcg
    18061 gggcctgatc ctggccagct tttcacgcgc ttcccaggcc acactgtgga tggcgtggta
    18121 atccaggttt tccggcaggc tgatttgttc ggagctgttg aaacgctcca attcagcgct
    18181 ggcccggtcc agatagccct catacttgat ctccagctca atgcggccgg cgatgtcgga
    18241 agtcagttcc gcggagggtt gatagccaaa gcggcggagg tcttcaaagg ctatctccgg
    18301 tcgcttgagc aggatggcga accgggtggg ctctttgggc agctcaggca cctgttctga
    18361 attgtaggat tcctgcccgg cttcattcct gaggacggaa cggcagaaca ggctcttttc
    18421 ggtcttgagg cgctccagtt ccctctcctt gatgcgcagc atctccagaa agcgctgcca
    18481 gcggatttca gagaccaggc ctagctcgtg accgattctc atcagacgct cgtcggcatt
    18541 gtcctgcctg agcaacaaac gatactctgc ccgggaggta aacatcctgt aaggctcgtt
    18601 ggtgccgcgg gttacgaggt cgtccaccaa cacccccatg tacgcctgat ctcttccaag
    18661 gatgagggaa tcccggccct ccagccataa agtcgagttg atcccggcca gcattccctg
    18721 agccgcggct tcctcataac ccgaggtgcc gttgatctgt ccggccaggt ataagccctt
    18781 aatgcgtttg ctcttcaggg aagcgtcgat ctcatcggcc ggcacgcagt catactcgat
    18841 ggcataggcg tagcgcatga ttctggcctg ctcgaggcct ggaatgctgc gcacgatccg
    18901 ggcctggatt tctggcggca gggaggtgga aatgccgttt acatagcctt cccaggtgtt
    18961 gagaccttcc ggttcgataa aaatctgatg gctgtcgcgc tgagggaact tgacgatctt
    19021 atcttcgatg gaagggcaat agcgaggccc gatacccttg atgatccctc catagagggc
    19081 tgaaagctgc aggttggcgc tgatgatgtt gtgggtctcg ggagtcgttc tggtaagaaa
    19141 acaggagacc tggtttttta gtaccacatc gcggtaatat gaaaaaccgc tgggatcggg
    19201 atcgccgggt tgctcttcca aaacgctgta gtccagggtt ctgaggtcca ctcggggggg
    19261 agtgccggtc ttgaagcgga taaggttaaa accgagtttt tccaatgagt tactgagctc
    19321 attggcagcc ggctcaccgc tgcggccacc ctctgaaata ctggtcccca catggatgct
    19381 gcccctcaga aaggtcccgg tggccaagat caccgccgga gcgtgatatt ccagtccgat
    19441 ggaggtgcgc agcccggtga ccgcgctatc ctttaccatg atttcgcaca cggtggattc
    19501 gatgatgtcg aggccgtcct gctcttccaa agctctgagc atcaactcac tatagagtct
    19561 gcggtcgttt tgggcccggg gcgcccagac ggctgggcct ttcttacggt tgagcatccg
    19621 aaagtgaatg ccgctgcggt ccgcgctata gccaagctct ccgcccagag cgtctatctc
    19681 tctggccaga tgacctttgg cgggtccacc catgctggga ttgcagctca tgcggcccac
    19741 gctttccagc ttgatcacaa agatggcggt cctggcacct ctgcgggctg aggctaaagc
    19801 ggcctcgatt ccggcatggc cggctcccac taccaggacg tcgtaatccg ctgtcacaac
    19861 gttccttaga tcaggcggcc ccgcatccag ccatagacct gttccatacc ggttttcttc
    19921 aacgcggaga cgggaaagat cggctccgtt ttgagcccca gtcctttagc cagcagctgc
    19981 agggtcttgg ggattttggt cttggggatc ttatccgctt tggtagccac cacgcagtgg
    20041 tcaatccctc tcagacgcag cagttccagc atcaggatat ctttgtcatc ggcggggtga
    20101 cggatgtcca ccagcacaat gatcgcctta agctgttccc gcacttcaaa ataacggttg
    20161 atcatcttgc gccacttctc ttgttcggcc tgtcccactt cggcaaagcc ataaccgggc
    20221 agatcggtga gttgcagctt gccttccgaa tctgtttctt cgtctttcca gcggaccaga
    20281 aagaaattga gcagcctcgt cttgcctggg gtggaggagg tcttggcgag accgtggacg
    20341 ccagtgatca ggttgatgag ggtggattta cccacgttgc tgcggcccgc gaaggcaaat
    20401 tcggcaaaag cgctggcggg atagtccacc ggttccacgg cgctcttgac aaagtagctt
    20461 tgcaccagcc taaacatgag aatacaccac ggcgctgcgc tcggattcct gaatctccac
    20521 gctttccacc tgcacgggtt ttccggccag ctcgcgctcg aagcgttcaa agacgtatct
    20581 ggcgaggttt tcggaggtgg ggttgtgctg tgtaaaggca ggcagctcat ttagataggc
    20641 gtggtcaagc tcatccagga tggaggccag gaactgcttt atcaccccaa aatccagggc
    20701 catcccgatc tcgtcgagct gatcggtagc gaggcagacc ttcactttcc agttgtggcc
    20761 gtgcagatta cggcaggcac cctggtatcc acagagccgg tgggcggcac tgaagctgtc
    20821 acttacgcta agtctataca atgcattctc ctaaaaatac ttttttctga taaagatcag
    20881 caccaggctg atcgaaacca acacgctgat ccccatcacg atggcaaagg cgtaagcgtt
    20941 ttcctgaaag ggcagatgca cgttcatgcc atagatgctg gcgatcagag tgggcaggga
    21001 aatgatgatc gtgatcgagg tgaggaactt catcaccacg ttgaggttgt tggaaataat
    21061 gctggcaaag gcatccatca taccgctcag gatgctggaa tggatgttgg ccatctcgat
    21121 ggcctgtttg ttttcgatga tcacgtcctc gatcatatcc tcggcttcgg ggtcctggtt
    21181 caaccagcgg ctgcgctgca ggcgttccag aatgatctcg ttgctgcgca gggaagtcga
    21241 gaaatagacc agggattttt ccatgcgcag cagccggata agttccttgt tgcgcataga
    21301 ttgatggagt tcgttttcga tgttgttggt gcggcggttg atctctttga gaaacttgag
    21361 ataatagatg gcggcgcgga ggttgatctg gagcaagaag ctctgctggg taatgaggaa
    21421 ggggcggtgc ttggcttcca gatactgagt gaggatctcg ctgtcgtaaa aagacacggt
    21481 gatcagcttg tcctgcacgg caataatgcc caggggggct gtcatgaaag acaggctggc
    21541 gctgcggtgc ctgtaataga tgggcacgcg gatgatgatc agggtggcgc cgtcctcata
    21601 ctccacccgg ctgttttcat ccgagtcctg cagatcggtg ataaaatcct cagggaggtc
    21661 ataacgctcg atcagggtct tgatctcatc gccgttggga ctgtccaaat gtatccaaaa
    21721 ggcctcgtgg tgagtaacgg ctggcacaaa acgcccttcc gctatcttaa aatactggat
    21781 catggcgacc tccttattca gattttgttt gaaagccaca attcctaagg ctttgatttg
    21841 tggcaataaa aaaaaacgaa gcgcgtccgg acaggcaacg gacgctggct caggttgctt
    21901 gaacgtagtt tcctggagcg ggaagaagcc tgtcctgcca tctgaacttc gttgatttta
    21961 ctgattcgct gccggcaatg aaaagcctca ctgttattcc tgcctccgcg agaatggcaa
    22021 gagatctgat gtggcttttc acgtaaactt ccacaccagc ttcgacctct ctcaagccgt
    22081 tgcagatgat ggatttagtc tgggctatga ggcttcctgg tattgggctg tcctgccggt
    22141 aaacgaggag ctggattctc ccgatccaga ttcggacgag tttatgatct ggcgtttcag
    22201 cactatggcc gatcccatgg gagaactgga agtacccaat gtgagcattg agcggctgga
    22261 caatagcgtg agagtctggt ggccagctgt gcctaacgcc tcaacctaca aggtgtatgg
    22321 cagtgtagac cccatattgg gctatagcct catcactcag acagccgata ccgaatggat
    22381 cgtcaccacc cctgggaaca tggagttctt caaggtagcg gcggttaatt aacagcatat
    22441 agacattcat aaagaagaca attccccggc ccggagctat atccgggctg ggcctttttt
//
LOCUS       DAOSFN010000020        22184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1883_length_22184_cov_9.160242, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000020 DAOSFN010000000
VERSION     DAOSFN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 22184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22184
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1883_length_22184_cov_9.160242"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLO35_02600"
     CDS             <1..218
                     /locus_tag="PLO35_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78583.1"
                     /translation="GMEALSWMELRILDGDLELLLPADMAVELAVEQASSIQNLHPHT
                     RIVPAGSPSQYRLFVPRGILSVKSADK"
     gene            285..881
                     /locus_tag="PLO35_02605"
     CDS             285..881
                     /locus_tag="PLO35_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOH78584.1"
                     /translation="MKKGYIIILVIVLLVIILAGGIIGRYNKMRVLKVNVQEAWGQVE
                     NQYQRRFDLVPNLVSTVQGAANFEKNTLTAVIEARSKVGQMTITPETLSDPQAFQKFQ
                     QAQDGLSSALSRLMVVVERYPDLKANQNFLQLQDQLEGTENRIATERVRFNDSVKLLN
                     QYVVVFPNNLLAGMFGIKEMPFFQASSGADMAPKVQFE"
     gene            1296..1913
                     /locus_tag="PLO35_02610"
     CDS             1296..1913
                     /locus_tag="PLO35_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78585.1"
                     /translation="MYTKKLLFLCLLAVMAVGLNAREDNTHSSIGLHFGTSSGNGYAM
                     RWMGPKLGLQATLGAYTSGSNDVKFNETYYDINDEYTDNEITINKKGRSTSGTVALNG
                     LIMLDHFNRGRLYIMAGGSYTYHRERVYSSRYIRSNYDNYNLVPNSKTSTRKTENRWT
                     AGIGPGIELGLSRHFRIAFEVPITYNYDDEIIMYIPQVGFYYYFN"
     gene            2017..2874
                     /locus_tag="PLO35_02615"
     CDS             2017..2874
                     /locus_tag="PLO35_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78586.1"
                     /translation="MKKLAMIALIAAGMCGLLKAESMYGAFQVETKPSGADVTLYDPD
                     VYLSQTPTPVYPVLMDEYMELREGIPGRSIMLMITKKGYVPLKRAIFVPFTHEADSLA
                     IREPSVFTFELERDVNNEHWRVCVYYGYRHRHPRPPQHVHFHPWYPPHYPHHPGWGTP
                     PPSPKPPVVYQGVIVSGTSEGPDLTASGSHSSDQPPSNEKPKPPKFQAPPPSKYVAPV
                     VDSKPSKNQPDKKSVSEVKKNDSPKPAKSEPAKIETNKSKPVVTPAPKTEKEEEKDTN
                     TKADKKSKK"
     gene            complement(3269..4999)
                     /locus_tag="PLO35_02620"
     CDS             complement(3269..4999)
                     /locus_tag="PLO35_02620"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOH78587.1"
                     /translation="MASQSSAEFYKNIKEMSPIRAIAETLLNNHNVRKIDIREAYEMA
                     RKQPGVSETDLPVYPEFVKLHKLPADTKVLNDCHGNILGRTAKARRFYHRLNPSQKNK
                     LEGDFREAVWQMMHYPLIKAEAILGMDKDLMIKATFVTTESDAANVYNWLLNFAPYEQ
                     LREQYEASPKLPIQDVILVAFNEWTCDDPFYNNVGAPQLALVDEKHNVIVNMGMRYFG
                     ERKKGTLTLAWTSGIRIGMAACHGGIKEIDFGTCEDPAYHKLGKRSIAFYGLSGTGKS
                     SHTNNADNAGTLPKGFSKAVLHDDAFQIDLENKLCRVWEPTLFDKTDSRPTDHPDWKY
                     ALALMNHMVMEIDGKRIPVGQDVRNSNGRALLDRSLLGKYVNRCAFPKALVWLMKDSV
                     LPPVIKLDNKYLAVAMGAALMTQRNRAENVAEDELNKLVFEPFANPFRVYELYRDVEA
                     FVKVVENGADVYCFNSRGYWKESDDVLEAIPLQTSLTMQTAILTDQLEWEKWEMLPGA
                     MIPTKASVDKVLPGYYDKYLPGARGNLDKYLATLKDRFQQRRDFLSGSDLTERPQLLE
                     EIVKTLQLNN"
     gene            complement(5044..6222)
                     /locus_tag="PLO35_02625"
     CDS             complement(5044..6222)
                     /locus_tag="PLO35_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOH78588.1"
                     /translation="MSITDKCYSFLDARRAIALGYYPYFREISSEQDTEVICNGQKML
                     MLGSNSYLGLTNHPRVKEASIKAIQKYGSGCAGSRFLNGTLDIHIELEAELAKLVGKE
                     AALAYPTGYQANLGCISAMVLKHEYVITDKLDHASIIDGCMLSQGTMLRYNHNDMKSL
                     EIVLKKIAGKNSLIVVDGIFSMEGDIADLPAICKLAAKYGANVMVDEAHSLGVLGATG
                     AGVAEHFGLIKESDFIMGTFSKSLASVGGFIAADEQLIHYLKHKSRALIFSASLPPAS
                     TASVLEALKIMAEEPERIQRLWDNTNYMLAEFKAMGYDTGTSCTPVIPLHVGDMMVAF
                     EMWKRLGDEGVFINPVVPPAVPPNSCLIRTSFMATHTRAQLDFALEKFRRIGKDLKVI
                     "
     gene            complement(6237..7298)
                     /locus_tag="PLO35_02630"
     CDS             complement(6237..7298)
                     /locus_tag="PLO35_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOH78589.1"
                     /translation="MNFTKVIFYLLLIAAIVSGVYFYSRVFGYIAMAMVLSYLLDPLV
                     SWVERRHLPRWMAVLCVYLGILGVLAWLSLGFVPDLVRQGRGLIDIMQTNTPGGENLL
                     VTLPFLRDVYQLLLNLDKQLPNLDLAAQFLTFLASAQTFLTTLPQTILDNYSEIIGAV
                     SYVGTVPLISFFLLKDKVLFRKAMVAAASNRYFELVIIFMNKIDETVGRYMRAMLLEV
                     IAVSIMVSIALTAIGVPNAVLIGVVAGVANIIPYFGPFMGAGVAILTVLVNGSPSIMI
                     LWVALTMYLVQVIDNNIVYPVVVGTTINMHPLIVLLTVLAGGWFGGILWMLISVPLVY
                     ICYSMLRVIYVNLREFRLL"
     gene            complement(7312..7779)
                     /locus_tag="PLO35_02635"
     CDS             complement(7312..7779)
                     /locus_tag="PLO35_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOH78590.1"
                     /translation="MNIKEYIRWLGKIIRKPDSFEAIKRISIFADLNSFQLHELNNYL
                     HTRHYKAGEVIFEQGYPLDAIFFIIAGEVRVSGKLSGSDPITLRENDFIGIIDMFHEG
                     TRSSTAMAVTEVNALAISRSDLTNLIARDKDMGLKILGAICRYLSSLNFSKSV"
     gene            complement(7781..8422)
                     /locus_tag="PLO35_02640"
     CDS             complement(7781..8422)
                     /locus_tag="PLO35_02640"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOH78591.1"
                     /translation="MRFDDQRALLIKELRLAGISDERVLQVFNTIPRELFVPSQYLEY
                     AYSNQPLPIDAGQTISQPLMIAIMLQYLELKPEHRVLEIGTGSGYQTALLAGLASEVC
                     SVERIELLSLKAQQVLKSQGVKNAFFRIGDGSQGWQRAYPPYRSFDRIIVSAGAKSIP
                     DGLTSQLAEGGILVIPVGKSGTQILHRVIRVQDELIISKHGACAFVPLISEQV"
     gene            complement(8412..9197)
                     /gene="surE"
                     /locus_tag="PLO35_02645"
     CDS             complement(8412..9197)
                     /gene="surE"
                     /locus_tag="PLO35_02645"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOH78592.1"
                     /translation="MKILLTNDDGVMAPGIRTLQARLVEAGHTVILIAPDRERSAASH
                     SLSLKRDLRVRMLAENEYSVDGTPVDCVVVALQQIIREPVDLVISGINAGQNMGEDVL
                     YSGTVGAALEAALTGLKAIAISINSYQDQIYDTAARWMVKLLELGIDELCMPHGVLNI
                     NVPNLSLDLIKGVRLTRTGHRRYYNFIRITEEHQDGFSYRIGGDSPVWEQVKGSDAEA
                     IAEAYVSITPLVFELTGAASFPQILEWLEEKQLLQLKGPADAL"
     gene            complement(9217..9576)
                     /locus_tag="PLO35_02650"
     CDS             complement(9217..9576)
                     /locus_tag="PLO35_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78593.1"
                     /translation="MSKKKYIVVQVLDPAEVDGYSDAQKTHYLSLGYLPCLLANGTTK
                     WLTESQRVIRAAQANKPFFRFSSKAQKPQKNRHTHRRHRSNFRKFMLDNWQFVSLLLV
                     LVAVLWALLTYWNVIFG"
     gene            9861..14480
                     /locus_tag="PLO35_02655"
     CDS             9861..14480
                     /locus_tag="PLO35_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78594.1"
                     /translation="MKYIVLGLILFLAALTTLSAVITPLQVIGPEQSKILPPGPDKFC
                     VYQDLLYLVDNSSGLVKVWNISKNRIQKSDLIKLPPKVLVNDVTRDDKNIFLLDSKNS
                     KIMIYDKDGQKVRDIATKGVPACAFKKAVRIQVNYQGIIYVLDQGRCVILRFTSEGLF
                     SGQAAVPGAFSFTLGADQQLRVLQLQQKRYYITVLDHALETLSFTEINDLKYGRDDLI
                     DLAVNDYGQYYFINGKKTQIGKLNQDLRLIPKSYFGSKNKTSNKTSFFEPSQIRVVSQ
                     KGADYVCVLDTRHTAIKIFTENDLSGLTKLERPMYQMRPVLSKTNVAPCLDFAVWDTL
                     SYVLSDGTMPNRKTSRQISCRDTNDKKKFNIFAASFKSEGVKSFDALTVYKNRLYVLD
                     KAAHRVHIFNSLDGSYEESFGDKGKYDGAMKNPGSIVADLRGNVYIADTGNFRISVWT
                     EYGAFQENINLKKDKLQPRQLRVDNYFMYVLANKGELYKSRLDDIQRDRITIASLPRI
                     SSFDLLEDSRVAIVKGDIQELVILSVDSTKPARSSTGRVCLGYKVDSRYFASSPSAEY
                     PFFSDIFQIRYNSRDRILYISDSKVKTSRTLKFIAPPLIPQYVAVDINDKLQTVILWD
                     QGDGIRRWSVNSYTDTGDTLTYMTSEPVYTVQKPQDTIRYYQVAAYSDDNKLGSYTDP
                     VIDHFSFARYLSETQNYLEAIKILEQMQDEHYMAKAVDELYRNYNELSDQYLKAGEYE
                     QALLALETASLQKGLSSDIIEKTVLIYKLMGTYKAGISFLNKLETASAPEWIKQRISL
                     YNLDKDYDNVIIQAEYYTRYHGDDQEVLSFLAQAFEEKAQYVEALDYTRKAAILNTTF
                     TDQMKIADLLLKLANYDEAIVHLNRMLTIYEDERDGIYNMLGKSYFGKGDWGQAADNL
                     ENALIINPESAEYHLNLAMTYDKDRKPQEAQVHFQESWRLNPKDFDTGVAFARFLQRN
                     NLGSDALTVIDQIMTFATDEQKDGQFHSLYGDLLTQNGRYDEAVAELALAVDLFPDND
                     GLYEMYQQSLQTRERENKFRNPLEIKEVKFDALYPSMQQYYRDHPIGTVTIYNTRNTT
                     INNVKLTVIIEDITDSWINIPVARVQPNEIVDVNIFAPINKNIFAVSKDSNRDINTEV
                     RIEYFFEDEGKSHKEIRRLQVLRIQAMDWSNRKQLGCFVNPNDENLRAFVVGVVLPTY
                     NQIELPKTSRNLVYAAQLYDFYHANEVRYVPDPSSSNSSGVSNDYIQFPYQTLELKRG
                     DCDDLLVLLASSLSVAGIETGFLDVPGHVILVVDSGLTSTDIMQSGFDINHFIYRNNK
                     YWIPVETTLLGKNNFVDSWLYAIESYRDIYNREQIFPDLIEFSQSHRIYPPASHTGVI
                     LSASFTRAEQARELFNRDIANIMLLNQITVEKDFIRANKQYPDNLDLLLQYALWSFDH
                     GKLQQAENLLAQILQAEPSNFAALINLGNLHLEKGEYDKARSRYVEALKANFETDKVN
                     LNLCVLEYRRSNLERAREYYNKIVDKDILYKISPQMYSDLVGKGE"
     gene            14482..15183
                     /locus_tag="PLO35_02660"
     CDS             14482..15183
                     /locus_tag="PLO35_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78595.1"
                     /translation="MQASHLTPSSTPSIRALLIVLLVVAATILTAAPVGKIRFILGDV
                     KYKENQNAPWKKATLNQSVEATASFKTGVDSKVEIIFNSNQSFIIQAKNTIAVKKIQQ
                     DMQSQQKWTSQVREKASNMSLQNKQKNTSVAGIRREEAEIKKSDLYWYIPPRQEIQEA
                     IDLMDLKEFQQAIPKFELVIEQGPLKKEAEIARTMLIIIYDELKDKPRQKLHYDALKR
                     DFPQSELLRSMPELK"
     gene            15183..17360
                     /locus_tag="PLO35_02665"
     CDS             15183..17360
                     /locus_tag="PLO35_02665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOH78596.1"
                     /translation="MRRHFRSALIILIAWIVARSLIVLPFTAPFVKMLDLGIYDTVLK
                     IDRFLKTKINQPIYDSIVIVDIDDKSLSQLGQFSQWPNQYFADATNILNMDAPKLIAY
                     DVMFADSSNFTRHSRDRITNSITDSRMRGYSEHFFEMINGDDEFAQAIFDAGNVFLAM
                     FNNPNLDILPPLPPSITPWDVSPRYALEASHPHPPIPVLADVAYGVGFAQVSVDESSK
                     IHDFPLFIRFGKEYFVNFSFQACLDLLAVDSIKVDRSKCRLYSKHKQVRILPLSPNGK
                     FYLKYPHQSPAFRTVSFVDLINQRVSPGYFYDKIVLIGSSATGLGDTKSVPFSSELPG
                     VELHATLIRNILENEFVTWNYQIWDFFWLALLLGVLMLLIKTGKPLIQLGWILGASLL
                     LFIYFIWLYNARGSTMYYSRLVLPWLICGVTLVFDHYNRQVRERKMVHDAFGHFVSKD
                     VIAEVMKDKDALKIGGKRRYVSALITDIRGFSTYCEKCEVDEITSFINQFFNRATNLI
                     IEQKGLLDKFMGDAILALFNVPYATPDYAIHACRTALLIQATAQEQQQEYREHPILHS
                     FKIGVGISSGEVIVGNIGSDTIFNYTGIGTSMNMCSRLEALNKVYETLIIVDESTYRM
                     ANDHFCFRHLDYTSVKGIKHPVHIYELVGEVQDTDSQYSEFMEIYGEGVDLMAEKRFE
                     EAKSSFQKAATMRPEDFATQMMIERLDIMNWETWNGVWEQKHK"
     gene            17478..17747
                     /gene="rpsO"
                     /locus_tag="PLO35_02670"
     CDS             17478..17747
                     /gene="rpsO"
                     /locus_tag="PLO35_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOH78597.1"
                     /translation="MPLTPEAKTAIMAEFKLHESDTGSPEVQVALLTKRINAITAHLK
                     EHAKDFSTRRGLLKLIGQRRRLLDYLKRKDIDRYRQVIKALGIRK"
     gene            17938..20157
                     /locus_tag="PLO35_02675"
     CDS             17938..20157
                     /locus_tag="PLO35_02675"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOH78598.1"
                     /translation="MHNFNIISREIDLCGRKLYVETGRMAKQANGSVFIKYGDSAVLV
                     TATMGKEPAENQDFFPLTVDYIEKMYASGKIPGGFFKREAKPTTSATLSARVIDRSIR
                     PLFPDGFRYPVHVVLNVLAYDGVNDPQVLGILGASLALGISDIPFHGPIAGCTVGYID
                     GQLVVNPSISDLEESSKLNLSVGGSASSVVMIESAAQELDEELMLDAVYTGHEEIKKL
                     CALQDDFISEAGKAKVEVILIRIPEEIISRVEADFGSRIAEAAVIFGKQERQDAFDAA
                     EAEMLEKYLAADGEEVFAEKEKWYKDAYEELIRRYVRESILKKHHRVDGRGLDDIRDI
                     TCEIDVLPIVHGSALFTRGETQSLGAVTLGGGSDEQVIDGLEIEYKKHFYLHYNFPPF
                     SVGEAGRMGPTGRRELGHGNLAERALKAVLPDREAFPYTLRIVADTLESNGSSSMASI
                     CSGCLAMMAGGVPIKAPVAGIANGLIMEGSDYVVLTDIMGLEDHLGDMDFKCAGTRQG
                     ITAMQMDIKVEGITKDIMRIALQKANTARNRILDIMRDCIAEPREDLAPTAPRIEAFK
                     VPVDKIGEIIGPSGKMIKSIIEICQVEIDISDDGTVRILSPNKDSIMKAKDMIRGIAV
                     EPEIGQEFEGPVTRIEPYGVFVKILGGVKEGMVHVSQMHTSRVEHPEDMVKLGDIVHV
                     KSLGSEKGKLSLTMKGVEGNPEPRPGQPRERSYPPRRDDRGGDRDRRPQGRGDRFRR"
     gene            20255..21661
                     /gene="ahcY"
                     /locus_tag="PLO35_02680"
     CDS             20255..21661
                     /gene="ahcY"
                     /locus_tag="PLO35_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOH78599.1"
                     /translation="MNNWIADIKLKELGRKEIDIAEIEMPGLMALRAKYGHDKPLSGA
                     RITGSLHMTIQTAVLIETLRELGAEVRWASCNIFSTQDQAAAAIAEQGIPVFAFKGET
                     LEEYWHCTLHALSWPNGQGPDLIVDDGGDATLMVHEGYRLEELYAATGTLPQADSEIH
                     EMRLVQELLISGISDDPLKWHRTVQNLRGVSEETTTGVHRLYQMLTDGKLLFPAINVN
                     DSVTKSKFDNLYGCRESLADGIKRGTDVMVAGKIVMVCGYGDVGKGCAQSMRGFGARV
                     IVSEIDPICALQAVMEGFEVNTLDNVVEEADIFVTATGNCDVIRVDHMMKMKDHAIVC
                     NIGHFDNEIQVIKLYELEGVRKINIKPQVDRIMLPNERSIILLSEGRLVNLGNATGHP
                     SFVMSCSFTNQVLAQMDLWQNAHAIGVYTLPKELDEEVARLHLEKLGVILTHLTTRQA
                     SYIGVPVEGPYKPEHYRY"
     gene            21661..>22184
                     /locus_tag="PLO35_02685"
     CDS             21661..>22184
                     /locus_tag="PLO35_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOH78600.1"
                     /translation="MSAHDPVIIEELFGELQVSGDDRQWIVTRTKARREKKLADQARR
                     NGIHYYLPQQSSNKVYQKRKVKFTKPLFPGYVFICANHAQKQTLAITGLTAGFITVTH
                     QEQLLSELRALRKLPVEKVEVGHQYWLSKGLEVEIVKGPLAGTRGIVESHDKIHEIRL
                     QVNILRQAVLVKVEP"
BASE COUNT         5696 a         5764 c         5601 g         5123 t
ORIGIN      
        1 gaggcatgga agcgcttagc tggatggagc ttagaatcct ggatggcgat ctggaactgc
       61 tcttgccagc ggatatggca gtcgagctgg ccgtggaaca agcctcctcc atccaaaatc
      121 tgcatcccca cacacgcatc gttccagccg gcagcccctc tcaataccgc ttgttcgtcc
      181 ccagaggaat actcagtgtg aaaagcgccg ataaataaat gtggacaaaa aggcgcgggt
      241 ctcaatgctt gcgccagatt ttaagtgaca aggagaaagc aaaagtgaaa aaaggataca
      301 tcattattct ggtgatcgtg ctgctggtga tcatactggc gggagggatc atcggacgtt
      361 acaacaagat gcgcgtcttg aaagtgaacg tgcaggaagc ctggggacag gtggaaaacc
      421 aatatcagcg ccgttttgat ctggtgccca atctggtatc aacggtgcag ggagccgcca
      481 attttgagaa aaacaccttg accgcggtga ttgaagcgag gtccaaggtg gggcagatga
      541 ccatcactcc cgagacgctt tcagaccctc aggcgttcca aaaattccag caggcgcagg
      601 atggcctcag cagcgcgctc agccgcctca tggtagtggt ggagaggtat ccggatctga
      661 aggccaatca gaacttcctg cagcttcagg atcagttgga aggcacggaa aaccgcatcg
      721 ccacggaacg ggtgcgcttt aacgacagcg tcaagctcct caaccaatac gtggtcgtct
      781 ttcccaacaa cctgctcgcc ggcatgtttg ggatcaagga aatgcctttc ttccaggcca
      841 gcagcggagc cgatatggcc cctaaggtgc agttcgagta gtatactctc accaatagct
      901 tatgcggcca tcgggaatca aagtcctgat ggccgtttgt ttctgaatca caccaattct
      961 ttccagcctc ctttgcctct cctttgcatc acgtttgcct caactttgct atggcatagg
     1021 tggagagcgg cgggagggtg agtaagaagc aagtcagcct gcttgtaaca ctataccgga
     1081 taacccgctc cagcataatc ttacaggctg ttggcacgca gtttgctcca ctccgggatt
     1141 ctgcatttga gtgatttcac accacccctg cagggcctga taaaatggag caaatcacct
     1201 cacacgcgct tgcagatgct gaaatttgca ttggcgcttg acaagattta ccgaaactgc
     1261 cagcttggca ctaactagct attaagaggt gtaacatgta tacaaagaag ctattattcc
     1321 tgtgcctgct ggctgtgatg gcagtgggcc tcaacgctcg cgaggacaac acgcattcct
     1381 cgataggact gcattttggc acatcttccg gcaacggtta cgcgatgcgc tggatgggac
     1441 ccaagctagg cttgcaggct actctgggag cctacaccag cggcagcaac gacgtgaagt
     1501 ttaacgagac ctattacgac attaatgatg aatatacgga caacgagatc acgatcaaca
     1561 aaaagggacg cagcacctcc ggaaccgtgg ccctgaacgg cttgatcatg ctggaccact
     1621 tcaaccgcgg tcgcctctat atcatggcgg gaggatcgta tacctatcat cgtgagagag
     1681 tgtattcctc cagatatata agatccaact acgataacta caacctggtc cccaattcca
     1741 aaacctccac ccgcaagacg gaaaatcgtt ggacagcggg tatcggcccc ggcatcgaat
     1801 tggggctcag ccgtcatttc cggattgcct ttgaggtccc gatcacttac aactatgatg
     1861 atgagatcat catgtatatc ccccaggtgg gattctatta ttacttcaat tgatagcatc
     1921 aatttctgct ccactctccg gatcacaaac tgagatgaag ggattggaaa tcgctccggt
     1981 attagatata gaactatcaa gctaaaggag ctgaacatga agaagcttgc catgatagca
     2041 ctcatcgccg ctggtatgtg tggcttactg aaagccgaat cgatgtatgg agctttccag
     2101 gtggaaacca aacccagcgg ggcggacgtg actctttatg acccggatgt atatcttagc
     2161 cagaccccca caccggtgta tccggtgctg atggatgaat acatggaact gagggaaggg
     2221 atcccgggac gcagcatcat gctgatgatc accaaaaagg gatatgttcc attgaagcgc
     2281 gccatcttcg tgccctttac ccacgaagct gattctctgg caatcagaga gccgtcggtg
     2341 ttcacctttg agctcgagcg cgacgtcaac aacgagcact ggcgggtttg cgtttattac
     2401 ggttatcgtc atagacatcc caggcctcct cagcacgtgc attttcatcc ctggtatcca
     2461 cctcactatc ctcatcatcc cggctgggga acgcctcctc cttctcccaa gccccccgtg
     2521 gtgtatcaag gtgtgattgt gagcggtact tcggagggac cggatcttac tgcttccggc
     2581 agtcacagtt ccgatcagcc tcccagcaat gaaaaaccca agccacccaa gtttcaagca
     2641 ccacctcctt ccaaatacgt ggcaccggtg gtggactcca aaccctcgaa aaaccagcct
     2701 gacaaaaaga gcgtttccga agtcaaaaag aacgacagcc ccaaacccgc taaatccgag
     2761 cctgccaaaa ttgagacaaa caagtccaaa ccggtagtca ctcccgctcc caaaacagaa
     2821 aaagaggaag agaaggacac caacaccaaa gcggacaaaa agagcaagaa gtgattgagg
     2881 tctccatacc atagcctctg agtaaatcaa ttcaagccgc tgggttccag ccagtcgcgg
     2941 gcaatagttt acgcctgagt aaactaaata cagcctcaga attggggttt ttgcactggc
     3001 tctgatgctg gtgattacaa ttgttcaata gtctcagagc gcggtttcgc agagcttgtt
     3061 tcctgccacg cccttattca tggatcggga gcccgtcctt atctccccca aggcatcccc
     3121 aataaagttc gggatgggta ggagcaggta gagggggccg caggatcgag gggtaaggga
     3181 ggatatggct atgtgcaaca ttcaaagtga gttatgaaaa cataaaaccc ccggccaaaa
     3241 aaccgggggt tcatcattaa ggagtggatt agttattcaa ttgcagagtc ttgacgattt
     3301 cctccagcag ttggggacgc tcagtcagat cgctaccgct caggaaatcc cggcgctgtt
     3361 ggaacctgtc tttgagggtg gcaagatatt tgtccaagtt gcctctggct ccaggtaagt
     3421 atttgtcata atatccaggg aggaccttat cgacagatgc tttggtaggg atcatcgcgc
     3481 ccggcagcat ctcccatttt tcccattcca gttggtcagt caggatggct gtctgcatgg
     3541 tgagagacgt ttgcagcgga atggcttcca gcacgtcatc tgattctttc cagtagccgc
     3601 gggagttaaa gcaatagacg tcggccccgt tctccaccac cttcacaaag gcttccacgt
     3661 cgcgatagag ctcatagacg cggaagggat tggcaaaggg ctcaaagacg agtttgttga
     3721 gctcgtcttc cgcgacgttt tcggcgcggt ttctttgcgt catcaaagcg gcacccatag
     3781 ccacggccag atatttgttg tcgagcttga tcacaggagg caggacgctg tctttcatca
     3841 gccagaccag ggctttgggg aaggcgcagc ggttgacgta tttacccagc aatgaacggt
     3901 ccagcaaagc acggccattg gaattgcgca catcctgtcc caccgggatc cgcttgccat
     3961 cgatctccat caccatatga ttcatcaacg cgagcgcgta tttccaatcg ggatggtcgg
     4021 tgggacggct atcggtcttg tcgaagaggg tgggttccca cacgcggcag agcttgtttt
     4081 ccaggtcgat ctgaaaggcg tcgtcatgca gcacggcttt ggaaaaaccc ttgggcagcg
     4141 tgccggcgtt gtcggcattg ttggtgtggg atgattttcc cgtgccggaa agaccgtaga
     4201 aagcgatgga tcgttttccc agtttgtgat aggcagggtc ttcacacgtg ccaaaatcga
     4261 tctctttgat gccaccgtga caggcggcca ttccgatcct gataccggaa gtccatgcca
     4321 gggtgagagt gcctttcttg cgctcgccaa aatagcgcat gcccatattg acgatcacgt
     4381 tgtgtttttc atccaccagg gccagttggg gggctccgac gttgttgtag aagggatcat
     4441 cgcaggtcca ttcgttgaaa gcgacgagga tcacgtcctg aatcgggagt ttggggctgg
     4501 cttcatactg ctctctgagt tgctcgtagg gggcgaagtt taagagccag ttatagacgt
     4561 tggcggcgtc ggattcggtg gtcacgaaag tggccttgat catcagatct ttgtccattc
     4621 ccaatatcgc ttcagctttg atgagcggat agtgcatcat ctgccagacg gcctctctga
     4681 aatcgccttc cagcttgttt ttctggctgg ggttcaggcg atgatagaag cgtctcgctt
     4741 tggcagtgcg gcccaggatg ttgccgtggc aatcattcag aactttggta tcggcgggga
     4801 gcttgtgaag tttgacaaat tcgggataga cgggaaggtc agtttcagat acacccggtt
     4861 gtttgcgggc catttcatag gcttcccgga tgtcgatctt gcgaacgttg tggttgttga
     4921 gaagcgtttc ggcgatcgcg cgaatgggag acatctcctt gatgtttttg tagaattcgg
     4981 cgctgctttg gctggccatg attcctccat gtattttatt tgttgtaaac ttatgtgtat
     5041 gagctagatc accttgaggt ctttgccaat gcggcggaat ttctctaggg caaaatcaag
     5101 ctgcgctctg gtgtgggtgg ccatgaaaga ggtgcggatc aaacaggaat tgggcggcac
     5161 ggcaggcggc acgacaggat tgatgaatac tccctcgtca cccaggcgtt tccacatctc
     5221 aaaagcgacc atcatatcac ccacatgcag tggtatgacc ggggtgcagg aagttccggt
     5281 gtcgtagccc atggctttaa actccgccag catgtagttg gtgttgtccc acagcctttg
     5341 gatgcgttcc ggttcttcag ccatgatctt gagagcttcc aacacgctgg cagttgaggc
     5401 tgggggcagg gaagcggaaa agatcaaagc gcgggattta tgcttgagat agtggatcag
     5461 ctgttcgtca gcggcgatga aaccacccac ggatgccagt gatttgctga aagtgcccat
     5521 tataaagtcg gattctttga tcaggccaaa atgctccgcc actccagctc ccgtagctcc
     5581 taaaacgcca agggaatggg cttcgtccac catcacgttg gccccgtatt tggcagccag
     5641 tttgcagatg gcgggcagat cggcaatgtc accttccatg gagaaaattc cgtccaccac
     5701 gatcaggctg tttttaccgg cgattttctt gagcacgatc tcgaggctct tcatatcgtt
     5761 gtgattatag cgcaacatcg tgccctgtga cagcatgcag ccatcgataa tggaagcgtg
     5821 gtccagttta tctgtgatca cgtattcgtg tttgagcacc atggcgctga tgcaacccag
     5881 attagcctga taacctgtag gataggccaa cgcggcttcc ttgcccacca gtttcgcgag
     5941 ctcggcttcc agctcgatgt ggatgtccag cgtcccgttc aggaatctgg agccagcgca
     6001 tccgctgccg tatttctgga tggctttaat agacgcttct ttgactctgg gatgattggt
     6061 gaggcccaga tagctgttgg aacccagcat cagcatcttc tggccattgc agatcacttc
     6121 tgtgtcctgt tcagagctga tttcgcgaaa atatggataa tagcccagtg ctatggcacg
     6181 gcgggcatcc aggaaactat aacatttgtc ggtgatactc atatattacc tctgttttat
     6241 aatagacgga attctctaag attgacatat atgaccctca gcatcgaata gcagatatag
     6301 acaagcggaa cggagatcaa catccacagg atgccgccaa accagccacc tgccaacacg
     6361 gtcagcagca cgatgagggg atgcatgttt atggtggtac ctacgactac cggatacacg
     6421 atgttgttgt ctataacctg caccagatac atggtgaggg ccacccacag gatcattatg
     6481 gaaggactgc cgtttaccag cacagtcaga atggcaaccc cggcccccat aaaaggaccg
     6541 aaatagggta tgatgttggc cactccggcc acgacgccaa tcaggaccgc gtttggcacc
     6601 ccgatggcgg tcagggcgat ggaaaccatt atcgataccg ctataacctc cagcagcatt
     6661 gccctcatat agcgtcctac cgtctcatcg atcttgttca tgaagatgat caccagttca
     6721 aagtaacggt tggaagcggc tgccaccatt gcctttctga ataagacctt gtctttcagc
     6781 aaaaaaaagc tgatcagcgg cactgtcccc acataggaaa cggccccgat gatctcactg
     6841 tagttatcca ggatcgtttg agggagggtg gtgaggaagg tctgagcgga agccagaaag
     6901 gtgagaaact gagccgccag gtccaaattg ggcaattgct tgtccagatt gagcaacaac
     6961 tgatagacgt ctctcagaaa gggcagggtc accagcaggt tttccccgcc gggagtgttg
     7021 gtttgcatta tgtctatcaa tccccttccc tgtctcacca agtctggcac gaatcctaga
     7081 gacagccacg cgagcactcc caggatgccc agatagacgc aaaggacagc catccagcgg
     7141 ggtaggtgac ggcgctcaac ccagctcacc agagggtcca gaaggtagga tagcaccatg
     7201 gccatggcga tgtatccaaa gacgcggctg taaaagtaaa ctccggatac tatggcagca
     7261 attagcagca gataaaaaat gaccttggtg aagttcatcc tgctgatata cctatacgga
     7321 cttggaaaaa ttcagggagc tcaggtagcg gcagatcgcg ccgagaatct tcaggcccat
     7381 atctttgtcc cgggcgatca gattggtaag atcgctcctg gagatggcca aagcgttcac
     7441 ttcagtaacg gccatagcag tactcgagcg agtgccttca tgaaacatat cgataatgcc
     7501 gataaaatca ttctcccgca gagtaatggg gtccgaaccg ctgagtttgc ctgagacccg
     7561 cacctcaccg gctatgatga agaaaattgc gtccaggggg tatccctgct caaaaatcac
     7621 ttcaccggct ttgtaatgtc tggtatgcag atagttgttc agttcatgca gttgaaatga
     7681 attgaggtcc gcaaagatac tgatcctctt gatagcctca aagctgtcag gtttcctgat
     7741 gatcttgccc agccaccgga tgtactcttt tatgttcata tcaaacctgc tcgctgatga
     7801 gcggtacaaa cgcgcaagct ccatgcttgc tgatgattag ttcatcctga acgcggatca
     7861 cgcgatgcag aatctgggtg ccggattttc ctaccggaat caccagaata cctccctcgg
     7921 caagctggct ggttaaccca tccgggatcg atttagcccc ggcactcacg ataatcctgt
     7981 caaaacttct gtagggggga taagctctct gccatccctg gctgccatca ccaatcctga
     8041 aaaaggcgtt tttcaccccc tgagatttta aaacctgttg cgccttgaga gagagcaatt
     8101 caatccgttc cacggagcag acttcagaag ccagcccggc aagcaacgcg gtctgatatc
     8161 cactgccagt gcctatctcc aaaacccggt gttctggttt tagctccagg tactgcagca
     8221 tgatggctat catcagaggc tgggaaatgg tctgacctgc atcgatgggc agaggttgat
     8281 tgctgtaggc gtactcaaga tactggctgg gaacgaagag ctcccgcggg atggtattga
     8341 agacctgaag cactcgctca tcgctaatgc ccgccagacg aagttccttg atgagcagag
     8401 cgcgctgatc gtcaaagcgc atcggccggg cccttcagtt gcagaagttg tttctcttcg
     8461 agccattcca ggatctgagg gaaactggcg gccccggtaa gctcgaagac cagcggggtg
     8521 atcgacacat aagcttccgc aatcgcctcg gcgtcgctgc ctttaacctg ttcccagaca
     8581 gggctgtcgc ctccgatcct gtatgagaaa ccgtcctgat gctcctcagt aatcctgatg
     8641 aaattatagt atctgcgatg accggtgcgg gtgaggcgca cccctttgat caggtcgagg
     8701 ctgaggttgg gcacgttaat gttgagtacc ccgtggggca tgcaaagctc gtcaatcccc
     8761 agctcgagca gcttcaccat ccagcgcgca gccgtgtcat agatctggtc ctggtatgaa
     8821 ttgatcgata tcgcgatggc tttcaaaccc gtgagagcgg cttccagtgc cgcgccgacg
     8881 gttccggaat agaggacgtc ctctcccatg ttctggcccg cgttgattcc agaaatcacc
     8941 aggtcaaccg gttcccgaat aatctgttgg agggcgacta ccacacaatc caccggagtg
     9001 ccatccacgc tgtattcatt ctctgccagc atcctgaccc gcaggtcccg cttcaggctc
     9061 agggaatggg aagcggcgct gcgttctctg tccggggcga tcaggatcac ggtgtgacca
     9121 gcctcgacga gccgcgcctg taaggtcctg attcccggag ccatcactcc atcgtcgttt
     9181 gtcaatagta tcttcaattt atgtcctttt gagctattat ccaaatatca cgttccagta
     9241 ggtcaataat gcccacagca ccgctaccag aacaaggagc aagctgacga actgccagtt
     9301 gtcgagcatg aacttccgaa agttgctgcg atgtctccta tgggtgtggc ggtttttttg
     9361 gggtttttga gctttgctgg aaaatctgaa aaagggcttg ttagcctgcg cggcccgaat
     9421 cactctctgg ctctccgtaa gccatttagt ggtcccattg gccaacaggc agggcaggta
     9481 tcccaggctc agataatgtg ttttctgggc gtcggaataa ccgtcaacct cagcgggatc
     9541 aagcacttgt actacaatat acttcttttt gctcattgat atagcctcat ttcttaattg
     9601 atttttgagg cgatttattt gtcaagcact atcttgatcg cgaccgtcaa tggctgcagt
     9661 tggcagccgg gaaccaacga gagcggttcc atgatacctg cgagcgggca tcgctaaagt
     9721 gacagggatt ggcattaata ccttaaacat tgttactatt tctgccagta ttcatccaca
     9781 ggaataaaga tgcttggttt tttgcttgac aagttagtac ctcccggaaa gctaagacca
     9841 aacagtctga tggagtgatc atgaaataca ttgtcttagg tttgatcttg tttctggctg
     9901 cgctgaccac gctgagcgct gttatcacac ccttgcaggt cattggtccc gagcagagca
     9961 aaatactgcc gcccgggccc gataaattct gcgtctatca ggatcttctc tacctggtcg
    10021 acaacagctc tggcctcgta aaagtttgga acatctccaa aaaccgcatc caaaaatcgg
    10081 atttgatcaa actacctccc aaagtattgg ttaatgatgt tacccgggac gataagaata
    10141 tattcctgct tgattccaaa aacagcaaga tcatgatcta cgacaaagac ggccagaaag
    10201 tgcgcgatat tgctaccaag ggagttccag cctgcgcgtt taagaaagcg gtgaggattc
    10261 aggtaaacta tcaggggatc atttacgtgc tggaccaagg cagatgcgtc atcctgcgtt
    10321 ttacctcgga agggttgttt tcaggtcagg ctgccgtgcc gggagccttt tcattcacac
    10381 tcggcgcgga ccagcagctc agagttcttc agctgcagca gaagcgctac tatatcacgg
    10441 ttctggatca cgctctggaa accctcagct tcactgagat caacgacctc aaatacggca
    10501 gggacgatct gatagacctg gctgtcaacg actatggtca gtattatttc atcaatggca
    10561 aaaagacgca gatcggcaag ctgaatcaag acctgaggct gatccccaaa tcatacttcg
    10621 gctccaagaa taagaccagc aacaagacca gtttctttga acccagccag atccgggttg
    10681 tcagccaaaa aggagctgat tacgtgtgtg tgctggatac acgtcacact gcgatcaaga
    10741 tttttaccga aaacgatctt tccggtctca ccaagcttga gcgccccatg tatcagatgc
    10801 gcccggtcct cagtaagacc aacgtggctc cctgcctgga ttttgcggtt tgggacaccc
    10861 taagttacgt gctctcagac ggcaccatgc ccaatcgcaa gacatccaga caaatcagct
    10921 gccgggacac caacgacaag aagaaattca acatctttgc cgccagcttc aaaagtgagg
    10981 gggtcaagtc gttcgacgcg ctgacggtgt ataaaaaccg gctctacgtt ctggacaagg
    11041 ccgcccatcg cgtgcatatc ttcaacagcc tggacggcag ttatgaagaa agctttggag
    11101 acaaaggcaa atacgatggg gcgatgaaaa atcccggctc aatcgttgcc gacctgagag
    11161 ggaacgtcta cattgcggac accggcaatt ttcgcatatc ggtatggacg gaatatggag
    11221 cttttcaaga gaatatcaat ctcaagaaag acaagctgca acctcgtcag ttgagagtag
    11281 ataactattt catgtatgtc ctggccaaca agggcgagct gtataaatcc aggctggatg
    11341 atatccagcg tgacaggatc actatcgcct ctttgcccag gattagcagc ttcgatctcc
    11401 ttgaagacag cagggtcgcc atcgtgaaag gagacatcca ggagcttgta atcctaagcg
    11461 tagacagcac caaacccgcc cgctcctcaa ccggaagagt gtgcctgggc tacaaagtgg
    11521 acagccggta ttttgcctcg agtccgtccg cggaatatcc gtttttctca gatatattcc
    11581 agattcgcta caactccaga gatagaatcc tgtatatcag cgactctaaa gtgaagacca
    11641 gcaggaccct gaagtttatc gctcctcccc tcatccctca gtatgtggcc gtggacataa
    11701 acgataaact acagaccgtg atcctctggg accagggtga tgggataaga cgctggagcg
    11761 taaacagcta caccgacacc ggtgacactc tgacctacat gaccagcgag cctgtttaca
    11821 cggtgcaaaa acctcaggac actatccgat actatcaggt tgcggcttat tcagatgaca
    11881 ataaattggg cagctacaca gacccggtaa tcgatcattt ctcttttgcc agatacctga
    11941 gcgagaccca aaactacctt gaagccatca agatattgga acaaatgcag gacgagcact
    12001 atatggcaaa ggcggtggac gaattatacc gcaactacaa cgagctatca gaccagtatc
    12061 tcaaggccgg tgaatatgag caggctttgc tggccctgga aacagccagc ttgcaaaaag
    12121 gcctcagctc cgacatcatt gagaagaccg tgctgatcta taaactcatg ggaacctata
    12181 aagccgggat ctcttttcta aacaaactgg aaactgccag cgctccggaa tggataaaac
    12241 agagaatatc cctttacaac ctcgataaag attatgacaa cgttatcatc caggccgagt
    12301 attacactcg ttatcatggc gacgaccagg aagttttgag ctttttggcc caggctttcg
    12361 aagaaaaagc ccaatacgtc gaagcgcttg attatacccg caaagccgct atcctgaaca
    12421 ccacatttac agaccagatg aaaatcgctg atctcttgct caaacttgca aactatgatg
    12481 aggcgattgt gcatctcaac cgcatgctca ctatttacga ggatgagcgt gacggcatct
    12541 ataacatgct ggggaagtcc tatttcggca agggggactg gggacaggca gccgataatc
    12601 tggaaaacgc gctcatcatc aatccggaat cagccgagta ccatctcaac cttgcgatga
    12661 cttatgataa ggacagaaag ccccaggaag cccaggttca ttttcaagag tcctggagac
    12721 taaaccccaa ggattttgat actggcgtgg cttttgccag attcctgcaa cgcaataatt
    12781 tgggcagcga cgctctcacc gtgatcgacc agatcatgac ctttgccact gacgaacaga
    12841 aagacggtca gttccactct ctgtatggag acttgctcac ccagaacggc cgctacgacg
    12901 aagccgttgc tgagctggcg ctggctgtag acctgtttcc cgacaacgat ggcctctatg
    12961 agatgtacca acagagcctg caaacccgcg agcgtgaaaa caagttccgc aacccgctcg
    13021 aaatcaaaga agtcaaattc gatgctcttt atccctccat gcaacagtat tatcgtgacc
    13081 atcccatcgg aacggtgact atttacaaca cccgaaacac taccatcaac aacgtcaagc
    13141 ttactgtgat cattgaagat atcaccgata gctggatcaa catccccgtg gcaagggtgc
    13201 agcccaacga gatcgtggat gtaaacattt ttgcccccat caacaagaac atattcgccg
    13261 tcagtaaaga cagcaaccgg gacatcaata ccgaagtcag gatagagtat ttctttgaag
    13321 atgaaggtaa aagccacaag gaaatcaggc ggttgcaggt gttgcggatt caagccatgg
    13381 attggagcaa ccgcaagcag ttgggctgct tcgtaaatcc caacgatgaa aatctccggg
    13441 ctttcgtggt gggagtggtt ctgccgacct ataaccagat tgaactgcca aagacatcca
    13501 gaaacctggt ttatgccgcc cagctttatg atttctacca cgccaacgag gtccgctatg
    13561 ttccggatcc cagctccagc aactcatccg gcgtctcaaa cgactatatt cagtttccct
    13621 atcagaccct ggagctcaag cggggagact gtgacgactt gttggtttta ttggcgtcat
    13681 cactgagcgt cgcggggatt gaaaccggct ttttggacgt ccccgggcac gtaatcctgg
    13741 tggtggacag cggtctcact tccaccgaca tcatgcagag cggattcgat atcaaccact
    13801 ttatctaccg taacaacaaa tattggatcc ccgtcgagac gaccttgctg ggcaagaaca
    13861 attttgtgga tagctggttg tatgccattg agagctaccg ggatatctat aaccgggagc
    13921 agatcttccc agacctgatt gagttttcgc agtcccaccg tatctatcct cccgcttcac
    13981 acaccggcgt gatcttatct gccagtttta ccagggcgga acaagctcgc gaactcttta
    14041 acagagacat cgccaacatc atgctcctga accagatcac ggttgaaaag gattttatca
    14101 gggcgaacaa gcagtatccc gataacctgg atttgctgct gcaatacgct ctctggagct
    14161 tcgatcacgg caaactgcaa caagccgaaa accttctggc ccagattctg caagccgagc
    14221 cctccaattt cgcggctctg atcaacctgg gcaacctgca cctggaaaaa ggtgagtatg
    14281 acaaagcccg ctcacgctat gtggaagctt tgaaagccaa ctttgaaaca gacaaagtga
    14341 atctcaatct ttgtgtgctg gagtatcgca ggtccaacct cgaaagggct cgcgaatact
    14401 ataacaagat cgtggacaag gatatcttgt acaagattag cccccaaatg tactccgact
    14461 tggtaggcaa aggagaataa catgcaagca agtcatttga caccctcatc aactccatca
    14521 attcgcgcgc tgctcatcgt gctgctagtg gtggccgcca cgattttgac agcggcaccg
    14581 gtcggcaaga tcagatttat cctgggtgac gttaaataca aggaaaacca aaacgctccc
    14641 tggaaaaagg caactttaaa tcaaagcgtg gaggcgacag ccagctttaa gaccggggtc
    14701 gattccaagg tagagattat atttaacagc aaccaaagct tcatcatcca ggccaagaat
    14761 acgatcgcgg tcaagaagat ccaacaggat atgcagtctc aacaaaaatg gacctcccag
    14821 gtgcgtgaaa aagcctccaa tatgagcctg caaaacaaac agaagaatac cagcgtggcg
    14881 ggaattcgca gagaggaggc tgagatcaaa aagagtgatc tctactggta tatcccgccc
    14941 aggcaggaaa ttcaggaagc gatagacctg atggacctca aagaattcca gcaagccatt
    15001 ccaaagtttg agctggtaat cgagcagggc cccctaaaaa aagaagctga aattgcccgc
    15061 acgatgctga ttatcatcta tgacgagctc aaagacaagc cacgccagaa gctccactac
    15121 gacgcgctca aacgtgattt tcctcaaagc gaactgctga ggtcgatgcc tgaattgaag
    15181 taatgcgccg tcactttcgc agcgctctga tcattctgat agcctggatt gtagcccggt
    15241 ctctgatcgt gctgcctttt accgctccgt ttgtaaaaat gctggatttg gggatctatg
    15301 acaccgtatt gaagatagac cgattcctga aaacaaagat caaccagccg atctatgatt
    15361 ccatcgtgat tgtggatatt gacgacaaaa gcctctccca gcttggccaa ttcagccagt
    15421 ggcccaacca atacttcgcc gacgctacca atatcctgaa catggacgct cccaagctga
    15481 tcgcctatga tgtcatgttt gccgattcca gcaacttcac ccgtcacagt agagacagga
    15541 tcaccaatag catcacggat tcacgcatgc ggggatattc tgagcatttt tttgaaatga
    15601 tcaacggtga cgatgaattc gctcaggcga tctttgatgc cggtaatgtc tttctggcta
    15661 tgttcaacaa ccccaatctg gatatccttc ctcctttgcc gccatcgatt actccgtggg
    15721 acgtctcgcc acgctatgcc ctggaggcca gtcatcccca cccacctatt ccagttctgg
    15781 ctgatgtagc ttatggagtc ggtttcgcgc aggtttccgt ggatgaaagc agcaagattc
    15841 atgattttcc actctttatc agatttggta aagagtattt tgtcaatttc agtttccagg
    15901 cctgtctgga cctgttggcg gtggatagta tcaaagtgga ccgatcaaag tgcaggcttt
    15961 actctaaaca caagcaggtt agaatcctgc ctctttctcc gaacggaaaa ttctatctca
    16021 agtatccgca tcagagtcct gccttccgga cggtctcatt tgtggacctg atcaatcaga
    16081 gagtatctcc aggctatttt tacgataaaa ttgtgctgat tggatcgtcc gcgaccggct
    16141 tgggggatac caaaagtgtc cccttttcct ccgaactccc tggcgtggag ctgcatgcca
    16201 ccctgatcag aaacatcctt gagaatgaat tcgttacctg gaactaccag atctgggatt
    16261 tcttttggct ggcgttactt ttaggcgtct tgatgctgct tatcaagact ggcaagccgc
    16321 ttatccagct aggttggatt ctgggcgcca gcctcttgtt gttcatctac tttatatggc
    16381 tttacaacgc ccgcggcagc accatgtact actcgcggct ggtgttgcca tggttgattt
    16441 gtggagtcac gcttgttttt gatcattaca accgccaggt cagagaacgc aaaatggtgc
    16501 acgacgcctt tggacatttc gtctccaagg atgtgatcgc ggaagtgatg aaggataagg
    16561 atgctctgaa aatcggggga aaaagacgct acgtctcggc tttgatcacc gacatccgag
    16621 gtttcagcac ctactgcgaa aagtgtgaag tggatgaaat cacaagtttt atcaaccagt
    16681 tcttcaaccg tgccaccaat ctgatcattg aacagaaagg cctcctggac aaatttatgg
    16741 gagatgccat tctagctctg ttcaatgtgc cctacgcgac tccggattac gcgattcacg
    16801 cctgccggac cgccctttta atccaggcca ccgctcagga acagcagcag gaatatcggg
    16861 aacatcccat tctgcatagc tttaagattg gtgtagggat tagcagtggc gaagtaattg
    16921 tgggcaacat cgggtcagat acgattttca actacactgg catcggcacc tcaatgaaca
    16981 tgtgttcacg tctggaagcc ctgaacaaag tttacgagac gcttatcata gtcgatgaga
    17041 gcacctatcg catggccaac gatcattttt gcttcaggca tctcgattat acttccgtca
    17101 aaggaatcaa gcatcctgtc cacatctatg aactggtcgg agaagtgcaa gacactgaca
    17161 gccaatactc tgagtttatg gagatctatg gggaaggggt ggatctgatg gcagaaaaac
    17221 gctttgagga agccaaaagt agtttccaga aggctgccac tatgagacct gaagattttg
    17281 ccacccagat gatgatcgaa aggctggata tcatgaactg ggaaacctgg aatggggttt
    17341 gggaacaaaa acacaaataa cgggaagccg tctctctttt ctcccccctt ccctttgatc
    17401 gcatcatctt cttaaaaaaa gcctttacaa aaatagcccg gttaaaagca tggctcaaac
    17461 ttcatctgga ggatgaaatg ccattaacac ctgaggccaa aacggctatc atggcagagt
    17521 ttaaactcca tgaatcagac accggctccc cggaagtcca ggtagccctt cttaccaagc
    17581 ggatcaacgc catcaccgct cacttgaagg aacacgccaa ggatttcagc acacggcgcg
    17641 ggctgttgaa actgatcggg cagcgcaggc gcctccttga ttatctgaaa cggaaagaca
    17701 tcgatcgtta ccgtcaggtc atcaaggcgc ttggcataag aaagtgataa ttctgccgga
    17761 ataattacct ggaagcggat cgctccaatt tcggcgtgat ccgctccccc tttatttgag
    17821 acaccactcc agccaacgga gtagaaactt aacccacttc gggagttcga gcaataagct
    17881 aagggaagcg ccctcagcct attgcttctt ctcccgcggt acaagaatgg agtaacaatg
    17941 cataacttca acatcatcag ccgcgagatc gatctctgcg gccgcaaact ctatgtggag
    18001 acgggcagga tggccaaaca agccaacggc agcgttttca tcaaatacgg cgatagcgcc
    18061 gttctggtta ccgccactat gggcaaggaa cccgcagaaa atcaggactt ctttcccctc
    18121 accgtcgatt acattgaaaa aatgtatgcc tctggcaaaa ttcccggcgg attctttaaa
    18181 cgtgaggcaa aacccaccac cagcgccact ttgtccgccc gcgtgatcga taggtccatt
    18241 cgtccccttt tcccggacgg atttcgctat cccgtacatg tggtcctgaa cgtcctcgcc
    18301 tatgacggcg tcaacgatcc tcaggtgctt ggtatccttg gtgcctcgct ggctttgggt
    18361 atctcggata tccccttcca tggtcccatt gctggatgca cggtaggtta catcgacggg
    18421 cagctggttg ttaatccctc catcagcgat ctcgaagaaa gctccaagct caatctctca
    18481 gtaggtggtt ccgccagctc cgtggtcatg attgaatccg ccgcccagga attggacgaa
    18541 gagctgatgc tggacgccgt ttacacaggc cacgaagaga tcaaaaagct ctgcgccctg
    18601 caggatgact tcatttccga agccggcaag gcaaaagtgg aagtgatcct cattcgtatc
    18661 cctgaagaga taatctccag agtggaagca gatttcggtt cccggatcgc tgaagccgcg
    18721 gtgatctttg gaaagcagga aagacaggac gctttcgacg ccgcagaggc agaaatgctg
    18781 gaaaagtatc tcgccgcgga tggggaagag gtctttgccg aaaaagaaaa gtggtataaa
    18841 gatgcttatg aagagctgat tcgccgctac gtccgtgagt ctattctcaa aaagcaccac
    18901 cgtgtggatg gacgcggcct ggatgacatt cgcgacatca cctgcgaaat cgacgtcctt
    18961 ccgatcgttc acggttctgc cctgttcacc agaggcgaaa cccaatccct gggagctgtc
    19021 accctgggtg gcggcagtga cgaacaggtc attgacggcc ttgaaattga atacaaaaaa
    19081 cacttctatc ttcattataa tttcccgcct ttcagcgtgg gtgaagctgg acgcatggga
    19141 cccaccggac gcagagaact ggggcacgga aaccttgccg agcgcgctct gaaagctgtt
    19201 ttaccagata gggaagcttt cccttacacc ctgcgcattg ttgccgacac cctggaatca
    19261 aacggttctt cttcaatggc ttccatatgc agcggttgcc tcgcaatgat ggctggcggc
    19321 gttccgatca aagctcctgt ggctggaatc gccaacggct tgatcatgga aggcagcgat
    19381 tacgttgtgc ttactgatat catgggcctt gaagatcacc tcggagatat ggacttcaag
    19441 tgtgctggta cgcgtcaggg gatcacagcc atgcagatgg atatcaaagt ggaaggcatc
    19501 accaaagaca tcatgcgtat cgctctccag aaagccaaca cagcccgcaa ccgcatcctc
    19561 gatatcatgc gggactgcat agctgagccg cgtgaagatc tggcccccac cgctccacgc
    19621 atcgaagcct tcaaggtacc agtcgacaag ataggcgaga tcattgggcc cagtggaaaa
    19681 atgatcaaat ccatcatcga gatatgccag gtggagattg acatttccga cgacggcacc
    19741 gtccgcatcc tctctcccaa caaagattcc atcatgaaag ccaaagacat gatcagaggc
    19801 atcgcggtgg aaccggaaat tgggcaggaa tttgagggtc ctgtcacccg catcgagccc
    19861 tatggagtat tcgtaaagat tctgggaggc gtgaaagaag gaatggtcca cgtttctcag
    19921 atgcatactt ccagagtcga acacccggaa gatatggtca agctgggcga tatcgtccac
    19981 gtcaaatcac tgggaagcga aaaaggcaag ctctctctca ccatgaaagg tgtggaggga
    20041 aatcccgagc ccagacctgg acaaccacgt gagcgcagct acccaccccg cagagacgac
    20101 agaggcgggg atcgtgatcg cagacctcag ggacgcggag atcgcttccg ccgataaaca
    20161 ccatcaattc ttaaattccg cggccctgtt accagtccac ctgttgacag ggcttgcggt
    20221 tgatataagg gacttttagt aaaaggagtc atcagtgaac aactggatcg cagatatcaa
    20281 gctcaaagag ctgggccgca aagagatcga tattgccgag attgaaatgc cgggcctgat
    20341 ggccttgcgg gcaaaatatg gccatgataa accgctttca ggggccagga ttaccggcag
    20401 tcttcatatg accattcaga cagccgtctt gatcgaaacg ctcagggaac tgggcgctga
    20461 ggtcagatgg gccagctgca atatcttttc cactcaggac caggctgccg cggccattgc
    20521 agagcagggc atcccggttt tcgcgttcaa aggcgaaacc cttgaagaat actggcactg
    20581 cacccttcac gctctcagtt ggccaaacgg ccaggggccg gacctgatcg tcgatgacgg
    20641 tggtgatgcc acgctcatgg tgcatgaagg ttatcgtctg gaagagctat atgctgccac
    20701 tgggacctta ccacaagccg attctgagat ccatgagatg agattggtgc aagaactttt
    20761 aatcagtggc atcagcgatg atcccctcaa gtggcatcgg accgtacaaa acttgcgtgg
    20821 agtgagcgaa gagaccacca ctggggtcca caggctctat cagatgttga ccgatggcaa
    20881 actgcttttc cccgcgatca acgtaaacga ctccgtgaca aaatccaaat ttgacaacct
    20941 ctatggctgt cgtgaatccc tggctgatgg catcaaacgc ggcaccgacg ttatggtggc
    21001 cggcaagatc gtcatggttt gcggatatgg cgatgtcggc aaaggctgcg ctcaatccat
    21061 gcgcggattc ggggcccgag tgatcgtgag cgaaatcgat cccatctgcg cgttgcaagc
    21121 tgtcatggaa ggatttgagg taaacaccct cgacaacgtc gtggaagaag ctgacatctt
    21181 tgtgaccgcc accggtaact gcgacgtgat tagagtggat cacatgatga agatgaagga
    21241 ccacgccatc gtctgcaata tcggtcactt tgacaacgaa atccaggtga tcaaactcta
    21301 tgaactggaa ggcgtgcgaa agatcaacat caagcctcag gtggacagga ttatgctgcc
    21361 caacgagcgc tccatcatcc tcctgtcgga aggcaggttg gtgaatctgg gcaacgcgac
    21421 ggggcatccg tcctttgtaa tgagctgttc tttcacaaat caggtcctgg ctcaaatgga
    21481 cctctggcaa aacgcacatg ctatcggtgt ttatacactg cccaaggagc ttgatgagga
    21541 agtcgccaga ctgcatcttg aaaagctggg ggtgatcctc acccatctta ctacccggca
    21601 agccagctac attggcgtgc cagtggaagg tccttacaaa ccggagcact accgctactg
    21661 atgtctgccc acgatcccgt gatcattgaa gagctgtttg gagaactgca ggtaagcggt
    21721 gacgacagac agtggatcgt tacccgcacc aaggccagac gagaaaaaaa actggccgac
    21781 caggcccgcc gcaacggaat ccactactac ttgcctcagc aaagcagtaa taaagtctat
    21841 cagaaacgca aagtcaaatt caccaaacct ctctttccag gctatgtgtt tatctgtgcc
    21901 aaccacgcac aaaagcagac acttgccatc accggtttga cagccgggtt cattaccgtg
    21961 acccaccagg aacaactcct ctccgagctg cgtgctctac gcaagctacc ggtggaaaaa
    22021 gtggaggttg gtcatcaata ctggctttcc aaaggcctgg aagtggagat cgtcaaaggt
    22081 cccctggcgg gcacgcgtgg cattgtcgag agccatgaca agatccacga aatccgtctg
    22141 caggtcaata tcctgcgtca ggccgttctg gtgaaggttg aacc
//
LOCUS       DAOSFN010000021        21566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1964_length_21566_cov_9.360327, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000021 DAOSFN010000000
VERSION     DAOSFN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 21566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21566
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1964_length_21566_cov_9.360327"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..4475
                     /locus_tag="PLO35_02690"
     CDS             198..4475
                     /locus_tag="PLO35_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78601.1"
                     /translation="MKRVLFVLVFFLLYSSGVWAALVLQGDISTDTTLLMSNNPHQIR
                     GTVRVLNGATLTVQPGVNLLFENGSQLEIQGAISAMGTSELPIWFHSIDADQHYTRVF
                     MNGAEGSSFYQCAFSRSRDFYGLLRIENSGPVNILTCDFDTTVNSHGIYIKNSQVIID
                     DIYISNIAHNGIQIESQEISGNPGSTVSLNDVYVNYCQTGVNIPQGNYPALTWGGITI
                     HHSSSYPLYANIFNYAALGSFSSSWEGIEMLAMWESYLYGSITLPYRSLPYYLLTNLN
                     LNNGAALTLEPGVGLRFPQYGRLQLYNGSSLNADGTELLPISFRAGGDYTWGGIGLDS
                     NCSALLDHCQFSSCGFPQYGYPEPSISANSISSLSISNTSIPGGGTYGIYVSGSNNGS
                     LSLDNVTIHDCPWTGLYILDRTLSLDYSNLAVTDCGRPIAIPPNLLNFLDEQPLFTDN
                     TDNRIFLHNEGYLYRDTTVRNWGYPYVCESINIDVNYVNFTINPGCEFQMGYSIGFYV
                     NGTITAVGTASQPILFNRLPGSTQNWRGFNLNAGTSYAHFDHCILQNCASSNQYSHIQ
                     DAFTIYRADTVLIENTQIIDAYCRAVFIESNDSDSDDLVINNLSIDGCGMDAVYQNAS
                     GYNLTINGLSVNGCNSFPVSVSANWAHQITGFTLTNNAYNVIRFVNGGYLASQTLANH
                     GYPYQISGGHLYVNYTNVSFLPGTVFYFERYRSLEVSGTLNAIGTADQPIVFDRPPGA
                     TYFWERIYLYNNSSASFQHCQFSNGGERNQYGYDNAMFDNAGATLLSLQDCVINDVDA
                     QAISCHDIGSGDTTQINNVQINGCRTDGFWCNDSDLTLSLDNLTVTGCLRNPISILPM
                     QTGCFDNLTLSGNASNDIRLFGYNGINGAVHFANHGYIYRCEEGLTGNSGSSVSFDPG
                     CVFWIADNRYVDFSGAVSAIGTADQPIVFTRYPVSTNYWIGVRVFGSSWDADFEHCQF
                     LYAGAQETYGSRRAFTDFGAGNLSISNCLIQNSFGDGFVVQETQSTDVLSISSLQILD
                     VAASGFVCNTSYHNISVNGLGITNCGSWPIYSSVDLLDKLTGVTITNPGTPYIGLGGT
                     TQTRSATWPNFGLPYRTNATLYVNDWVTLTISAGTEIIFGDYVQYSTDPRFLVYGALN
                     VLGTETNPVTMRGLNISAPSSWKGLRFYNPDAVCNLNYLNVYNAGLDEGDTPSEDFCA
                     LYIYNGTVNLTGCRVAISNHNLLKLEGNNSTTLSNCLLDSAVNGIIHNQGILNLYNNT
                     ISALSGTGIFQNGGTLNFGSSPGQWNRVYGNTLNLRNNTAAVLNAPYVYWGSLDPAVI
                     DATIWDNEEGSGTVNFEPWYDVNCLQLYYNTLDTPTGITVLQQTATSLRLSWDAVPAA
                     TSYKVLTAQDPYTSEWTILQQNISGTQIDLDINPALGKCFYKVVAVR"
     gene            4711..5367
                     /locus_tag="PLO35_02695"
     CDS             4711..5367
                     /locus_tag="PLO35_02695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH78602.1"
                     /translation="MIRGLIFDMDGTLLDTIEDIAIALNRALLDMGLTGHNLEECKTL
                     VGSGIENLAKAALPPDRHNEATIEELVLRFRAHYAQNWHESTQPYEGIESLLNTLVKR
                     GLHMAILSNKSHGFTSLMADHWFIRDGHQIFDPVWGERSGVPIKPDPSSSLAIAELWG
                     LKPSEIAFIGDSEADMLTAVHADMLPIGVAWGFRSVSQLMASGAKIILQSPEELLKYM
                     "
     gene            5860..8073
                     /locus_tag="PLO35_02700"
     CDS             5860..8073
                     /locus_tag="PLO35_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78603.1"
                     /translation="MKRYLLAVLLLTASLSLFAQQIPLPLLWEDLTYLGRTDCVIEGD
                     LMYMTNSLNAVSGPGLDLAILDLSASQRFKILGTLDLDAPAASRLAVHADRAIVIHTD
                     GRLSAIDISNPSQPLFITSTLSPAAIIAWESYGHYLYLYDSQGYMRCYAMDALDLVAS
                     SWIGTYYTAMTIESGRIYLSGTMPGFRCLGIDQPSAITDLGTLGNASNQQIMESGSGV
                     LICWTPGSALHFYDISSVGICNLIQTWSALIADYIRIDASGGRMAVYARITYSEYYES
                     SLKICDLEAAGGPQVIYTDDNPGTLSWLCISPDYLLHLYEYFGNGYTYSLYDLTNPSQ
                     PQYLDTLLTGGVSYLATNSNRLFANVGMQGLNIYACSETGAIVQTRLPEPWISYVAAS
                     DQHLLMNRVWYNGPGLPTSNAIYIYTILNDDEYEWVYYTGSCSLGDPITDVLYQDNRL
                     YMCGWGGLAIYDLTDTSHPDELARLPGSYFYLGVQGDYLYLTGYVNGSKLWIYDISAP
                     SSPQQLSWLAASYLDLGDLQYHNNVLFVGRQPMVVDVSDPSNPEVLHQYGTIVGSANA
                     LEVADNALIVMRWNDLRVFDISSIANPQLVSSTSFDTDANCMTVKDGLAYIGHKEGLS
                     CYDLRDALAYCAALSTPDDNNQVVPALSVYPNPMRDSSIMKLELPEAGDYSIGIYNLK
                     GQLLKRFPSQTYPAGSNTLTWTGLNDQGVKLPSGVYLLRASSPSRTIQAKVILLK"
     gene            complement(8081..11410)
                     /locus_tag="PLO35_02705"
     CDS             complement(8081..11410)
                     /locus_tag="PLO35_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOH78604.1"
                     /translation="MTRFKPLIITASAGTGKTYRLALEYVRLLLDHFDLPGFSMDSIL
                     VLTFTRKATAEIKQRIQDHLRLLVSTHSQDRDERRELILELKYRQGDGELSPSEEGIL
                     TSVSMVLAADRRMLQVMTLDSYIHSIFRNIVKPLRSIGDFELDLQAVKKRMPFLLSDL
                     MTPSRRAVVDSLLRRKVSPSLDAYEQFFTSLIDNRWLLYLLTRPVNPEGKATLRYHFL
                     HPDPAYAQAAKDGLFQALDQILQTVSDRALAKQKDWEELFNADTRKLFASFPPDPESF
                     LAELDPILERSSSAMALYKTIKELKLYNGNIIRANKPENSHLPELLQEARRQLANYLV
                     HSYLLPEQAEIFSIWEAILGSYDHLIYRYKNMTYNDIAWFTLEALFKGEPPNFELSEE
                     NTANEFYQFLSHRSRYILIDEFQDTSLIQFAILKPIIEEVCAGHGSQDYGGLIVVGDE
                     KQSIFGWRGGERELLLNLKQIFPTLKDIRQDRLDKSWRSSPLLMDFINSIFQDNGLKD
                     YLAQRGMKWDYEPITSAVPEINHLSQLSFDCEKYQTMNVQRSLDEVRRQWIKDWVMPY
                     CKADEEAGYSAKKPESIAILCRKGSELSQMQQLMDEFGITSVYQPNAELPEHHLIAPL
                     LSYLKWLAWKDWLDWLAWLRSDYILLRPQALKLAVDTIWKAGQQKPPIAPDFKDQPLL
                     HKFSQAELPMDSGVYAICQQLVDCYLDRGSLSERDQLNLDAFLSLTASFELDPSQTRK
                     DLPAFLEYLEDLRGQDALKQVAIEGGDKVQLLTIHKSKGLQFDRVFVFYNLSGRHGNE
                     GKKLDWYALFAGKDFNSISDFALSYHYGQLLEHSDYADLHTRQEQRALLEEMNNLYVA
                     FTRAKTKLHILFTYRSTKEWSDYRNDQDTKDDKLPNLLCEACHRWFDGQREKLGQPKP
                     GAPEPDKQGKQESDTPRLISCEASLLASALDFAPLPLAEPQQLDPAANRRQIWLEDRA
                     NLIGDLWHYYLSFIIRDLPEEHSHARRQVISHYCSILTLQRIDAELKKLRDRLRDFPL
                     LFEPRWNKVFTELPIYQGAKELRVDRLMLDTLAKQALIIDYKTGGIYETEQLERYRSV
                     LAQLPVLRDGAYSLEISYIQL"
     gene            complement(11407..14268)
                     /locus_tag="PLO35_02710"
     CDS             complement(11407..14268)
                     /locus_tag="PLO35_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOH78605.1"
                     /translation="MRSLVCLPFSADLNAAAIKEIVDPSLLIFPTLASASQARRLFQP
                     RWQLEEVDFISMEQLRQMLLPAPHPELQDEKRLLCLWQVLDETDKETFHLYCYDDLVS
                     WGQSFLSFFAEMRDELVDLELLQDPAQMDLGTRLWQEEHLKRVLAIRQRYHDFIFAKG
                     FTDSIFYLGLEQAVIPFSARKLVFVNQYYFSKLEKLLVERLEAYGCEIVFLHQGPQGS
                     FDPLKLESKPLELSALDQQDIRPRELKIIQAPDQDQMALACLCELNSHAETLAAPQTS
                     TRRVIVDGGFASKSYSRWFDPQRFRFSHALSMHSTRLYQLLSVYATHLKALAAAQNQR
                     FLPLAEMSRALAIPGFCQYYLPHWTETDRHQALDQLRRLAMEAVLYIDLDLNLFAADP
                     QDERYKLLIDLLGKHFALLDRLSQLASLEAVSACFDTPNGLDLKRLCSPEELASTDIL
                     DRFYERLANFGGIEELGLVADWKLFFNASGTALASGLWKLWMDHLAGASVSKYASPSA
                     PLYDIANLMDSRNLQYDEVIVLNCVEGILPSNPEAVWLLNEVQRKKLGLKHFELVRQW
                     ERYYFLRLVLGSQTTTLYCYRDEERDLDPGSYLSELEGHLSSQAWPELRCVRSETVLA
                     HDLLARARAILHPGPRFSDLEAQGLPGSSPTEDFFSLPCQPEQDFGTGIKSAYYGLSA
                     LAANPFAWYIQNLRGLKAVELLPRETISPMLFGTIMHSFLTDILLELQGQHQDTRRLR
                     AAFQSPESLSKKLRKLLGSAQFDHKLPQNYNRDFLFGVISDCLVRSVRDFYYQWLEPN
                     LYGYSFRLIPEQERMTNEEWQYKTLLSFGPQGEYKLEIHGKADLRISLPGQDFIVDFK
                     TGKGDAEQLIFYEYFYYRLDPEYQNQLNSLFWMILDARRDSENLSDKKRQSWRDGLIQ
                     ILDQCLSSGYGLGTKATDRERLKQITRADLWKAPSGRES"
     gene            complement(14284..15849)
                     /gene="cls"
                     /locus_tag="PLO35_02715"
     CDS             complement(14284..15849)
                     /gene="cls"
                     /locus_tag="PLO35_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOH78606.1"
                     /translation="MVSQKIWDSVLLIMILLISILLTASLLQWLTGGEFPLVFSNLAQ
                     KLLNIIFGVTILLMIVVLVLENDNPVHTLAWILILLYLPVVGFIFYLFFGRNWRKTRL
                     FNRKGLADAINLHDLWPSEGESYRRDLPALASRLVDLLDSNSKAILTSRNRVEIIPDT
                     NAALDQICADIEKARHHVHLEYFSIASDQTGKRVKDLLIKKAGQGVEVRFIYDDVGCW
                     DLRPSFKKELRAAGVLFVPFMPVWIPFLNSRANYRNHRKIVIVDGQKAYLGGLNIGNK
                     YLGQNRYYGYWRDSVVSIEGEGAVTLQAIFLTDWFFVSKQNLLTRQLFPKYVPPAQTD
                     PSSLEDIPLQIAASGPDTDHSSIMQAYFAAITNARKRIRIVSPYLILNESLLMAIKTA
                     AMSGVRVQIILPGKPDHLIVWWGGRSYYRELMEAGVRIYEYQNGFLHSKVLIVDDEVL
                     SIGTANMDLRSFNHNFEVTAMVYHPATVAKALEQFEADLLMSREVDLVKLQHRNIFSR
                     TMESVCRLFSPVL"
     gene            complement(16138..>19098)
                     /locus_tag="PLO35_02720"
     CDS             complement(16138..>19098)
                     /locus_tag="PLO35_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78607.1"
                     /translation="VGNIWLRVSNYGFFGSGTDANPKYPSLEYPGGSGIDYLFRGALW
                     FGAKKYRRDGAGRKLYWRAINPSADSTGTVAEGTSEWQPWMKPVIDTLVTLGFDGEWG
                     VYEMLPAYNPLVAGNPDAADLYESWNTVDAIATASTRKQKRAEDDDGDGTIDEDFLGY
                     TFPLRHGTELPAQFSLFGSHYIHETNNYAIITEGSNSEIWFPLGFMDLADRSFASYAF
                     NSPYDDDTDGRFDEDGAPVSEQDYVSHYYDYCPFGTAGDRDLGAHRSLNRHHPLNIRA
                     RQMSYQWSYDYIKNLVYVEFDITNMNIATGDTLFDCSMGIYMDADCGPQTWEAIYADD
                     MSGYVKGTGYEFAYTYDADKDGGLTSGMIGARVCTPDPELLKFHCWYWAGDDGPSDFD
                     PWNFNYAPLRTSNEKYWLLTGRNPDEARFQPLRPEQDDLTEYVQPSPNDTRFLFSFYG
                     AQPGTADYNEMLDGVYHKRWNLAPGRTMKIVVAVFPGEDKEDLKGSARWAKEIYGRAQ
                     TLVNVVLPDTFPHYNPPEPPPIPALYAELQDDGKRIDLWWDNRSEFAYDVKTVGSGII
                     GWQDPSGPQYLPGLDSDPLFIDWSGYPAEFQPAASAPNPNATVNPFTANRLRHDFQGY
                     SLWGRSGSGSQEDWVLIERWDKEDTYQDLEDYRVNIYSPYADSLFVDYGGYLGIDKGL
                     PNHNAWPESSQYQDYTCLDINYAFAANPAESFYGFPIYDQAVDWSEALEAQAVQIAED
                     HSGLPDDMIKDLQADIFRHPQMPEHIYKALYDSKMIPLPGHGGQVAIPHLGGSLEALQ
                     DLKDKRLARRFYRASIDHPRRGIEYYVALSAYDRGIPSHELNALETGRDADANMLIFF
                     PGTLARQNLDDVYVIPNPYLGRSKFDGRRENDEKGDKSRRLWFVNLPERCVIRIYTLA
                     GDLVKEIHHEGAYEADILTVSKAASQGLSASGMHAWDLLSRHRQIIAPGVYLFSVENR
                     ADGRKKVGKFVIIK"
     assembly_gap    19099..19198
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19447..20286)
                     /locus_tag="PLO35_02725"
     CDS             complement(19447..20286)
                     /locus_tag="PLO35_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOH78608.1"
                     /translation="MSQARIIKLVLTGQPSTDGAGVRLYRVFGYHQINQFDPFLMLDH
                     FGSNDPKDYLPGFPWHPHRGIETITYMKTGTAEHGDSIGNSGVIGPGDVQWMTAGSGI
                     IHQEMPKPDEKGRMFGFQLWTNLPASQKMMPPRYRDVLAAEIPEIKSSASHIKVISGE
                     VFGTRGPVQDIVTDPHYYIIRMPASDVLELPVDPSYTCFVYCYEGGIKIGPKIVSDHQ
                     VALLTDGDVVRIEATHKGGHFLFLSGKPLGEPVAWGGPIVMNTKEELRLAFKELEEGK
                     FIK"
     gene            complement(20348..21022)
                     /locus_tag="PLO35_02730"
     CDS             complement(20348..21022)
                     /locus_tag="PLO35_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOH78609.1"
                     /translation="MRNPLNEILTPSEQKALLFLALCFMIGTGLNLLGWKPSSPLLAN
                     QSLEDAPPESLATALKEDVPIIIDIRVATQAELEELPGIGAKRAQEIIAHRAKTPFNN
                     VNEIMLIKGIGLKTYAKMRPMLLVFGNAEPIDKNAKSPATKSDSPASKAEAQPKKADL
                     TNIVNLNTASLDELCTLSGIGPAKAKAIIEFREANGPFASIEDITKVKGIGPATLEKN
                     RSRLKI"
     gene            complement(21034..>21566)
                     /locus_tag="PLO35_02735"
     CDS             complement(21034..>21566)
                     /locus_tag="PLO35_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78610.1"
                     /translation="SMGVYSDLDPDFEDARWDEFEDRVPEYEELARQREKEEFEQQRL
                     RLQLPELDIQDANAVPLKLSDYLGQTLHLIFWASWNQRERGQLQVYLEESVSDSSGVV
                     TLLVNGYDSDPIRAQAQQMELGWEMPYLRIDDASLERLGIKYLPAVIEIGPDGLLDYI
                     NDGTDLRFWWERYPIH"
BASE COUNT         4843 a         5777 c         5881 g         4965 t
ORIGIN      
        1 tggacatttg aagttttggt gatgaaattt gaagtattca gtgagggttt ataacagtct
       61 caaggagcgg gatctcatag acaaatcaga ttctgcttga caagctttga tatgcttgcc
      121 aaatagaaac caccttctgg catatatccc ttttacgttt ataagcttat tatctatcag
      181 atataaagga gccaaccatg aaacgtgttc tctttgtttt ggtatttttt ctgctatact
      241 cgagcggagt gtgggccgcc ctcgtgctgc aaggagacat cagcaccgat accaccctcc
      301 tgatgtccaa caatccccat cagatcagag gaacggtgcg tgttctcaac ggtgccaccc
      361 tcactgtgca gcctggggtc aacctcttgt ttgaaaacgg ttcccaattg gaaattcagg
      421 gggccatcag tgcaatgggg acctctgaac tgcccatctg gtttcactcc atcgatgcgg
      481 accagcacta taccagggtc tttatgaacg gggcggaagg cagcagcttc tatcaatgcg
      541 cattcagccg ctccagggac ttctacggcc tgctgaggat tgagaacagc ggcccggtca
      601 acatcctcac ttgtgatttt gacaccacgg tcaattccca cgggatctat atcaaaaact
      661 cccaggtgat catagacgat atctatatca gcaacatagc ccacaacggc atccagatcg
      721 aaagccagga gatcagcgga aatcccggca gcaccgtgag cctcaacgac gtgtatgtga
      781 attactgcca gacgggtgtt aatatccccc agggcaatta tcctgccttg acctggggcg
      841 gcatcaccat ccaccacagc agttcctatc cgctctatgc caacatcttc aactatgccg
      901 ctctgggatc tttctcatca tcctgggagg gcatcgaaat gctggcaatg tgggaaagct
      961 atctctatgg cagcatcact ttgccctacc gcagcctgcc ctactatctg ttaactaatc
     1021 tcaacctcaa caacggcgcg gccctgactt tggagcccgg agtgggattg cgcttcccgc
     1081 agtatggcag gttgcagctt tacaatggat caagcctgaa cgcggatggc actgaactgc
     1141 tgcccatcag cttccgggct ggaggagatt acacttgggg agggattggc ctggacagca
     1201 attgctccgc gctgctggac cattgccagt tcagcagttg cggctttccg caatacggct
     1261 atccggagcc ctccatctcg gcaaattcta tctcctccct cagtatctcc aataccagca
     1321 tcccgggcgg cggaacttac ggcatctatg tgagcggcag caacaacggc agcctcagcc
     1381 tggacaacgt aactatccat gattgcccct ggaccggcct ttacatcctg gatagaactc
     1441 ttagcctgga ttactccaac ctcgctgtca cggattgcgg caggcctatc gccatacccc
     1501 ccaacctgct caacttcctg gatgaacaac ccctctttac agacaacaca gacaaccgca
     1561 tttttctgca caacgaaggc tatctctacc gcgacaccac ggtgcgaaat tggggatatc
     1621 cctatgtgtg cgaaagcatc aacatcgatg tgaactacgt gaacttcacc atcaaccccg
     1681 gctgtgaatt tcagatggga tattccatag gcttctatgt gaacggaacc atcaccgcgg
     1741 tgggaacagc cagccagccg atcctcttta accgcctgcc cggctccacc caaaactggc
     1801 gcggtttcaa tctcaatgcc ggcacttcct atgcccactt cgaccactgc atcctgcaaa
     1861 actgcgccag cagcaaccaa tatagtcaca tccaggatgc cttcaccatc taccgcgccg
     1921 atacggtgct gattgaaaac acccagatca tcgacgcgta ttgccgggct gtctttattg
     1981 aaagcaacga cagcgatagc gacgacctgg tgatcaacaa tctcagcatc gacggctgcg
     2041 gaatggatgc cgtctatcaa aacgccagcg gctacaacct tactatcaac ggcctgagcg
     2101 tgaatggctg caactccttc cccgtgagcg tatcggcaaa ctgggctcac cagatcacgg
     2161 gatttaccct caccaacaat gcctataacg tgatccgctt tgtgaatggc ggctacctgg
     2221 ccagccagac tttggccaat cacggctatc cctatcagat cagcggcggg catttgtatg
     2281 tgaactacac caacgtgagt tttctgcccg gaacagtgtt ttactttgag cgttaccgca
     2341 gcctggaagt ctccggcacg ctgaatgcca tcgggaccgc ggaccagcct atcgtgtttg
     2401 accgtcctcc gggagcgaca tacttctggg aacggatcta cttatacaac aactccagcg
     2461 ccagtttcca gcattgccag ttcagcaatg gcggcgagcg caaccagtat ggctatgaca
     2521 atgctatgtt cgacaatgcc ggagctactc ttttaagcct gcaagactgc gtgatcaatg
     2581 acgtggacgc ccaggccatc agttgccacg acattggcag tggagacact acccaaatca
     2641 acaacgtcca gatcaacggt tgtcgcacgg acggattctg gtgcaacgac agcgacctca
     2701 cccttagcct ggataacctc actgtgacgg gctgcctcag aaaccccatc tccatcctgc
     2761 ccatgcagac aggctgcttt gataatctca ctctcagcgg caacgccagc aacgacatcc
     2821 gcctcttcgg ttacaacggc atcaacggag ctgtccactt tgccaatcac ggttacatct
     2881 atcgctgtga ggaaggcctg accggtaaca gcggatccag tgtgagcttc gatcccggct
     2941 gcgttttctg gatcgcggac aatcgttatg tggatttcag tggagctgtg agtgccattg
     3001 ggaccgccga ccagcccatt gttttcacgc gctatcctgt cagtaccaat tactggatcg
     3061 gcgtgagggt atttggctca tcctgggacg ccgattttga gcattgccag ttcctctatg
     3121 ccggagccca ggaaacctat ggcagcaggc gagccttcac agattttggg gctggaaacc
     3181 tcagcatctc caattgcctc atccaaaaca gctttggcga tggattcgtg gttcaggaga
     3241 ctcagagcac ggatgtactg agcatctcca gcctgcagat cctggatgtg gcggcctcgg
     3301 gattcgtctg caacacctca tatcacaata taagcgtgaa cgggctcggc atcaccaatt
     3361 gcggttcctg gcccatctat tccagcgtgg acctgctgga taagctcact ggtgtcacca
     3421 tcaccaatcc cggcacaccc tatattggtt tgggtggcac tacccagacc cgcagcgcca
     3481 cctggcccaa ttttggcctg ccctaccgga ccaatgccac gctttacgtg aatgactggg
     3541 tcaccctcac catctctgcc ggcaccgaga tcatctttgg agactatgtg cagtattcca
     3601 ccgatccccg cttcctggtc tatggagcgc tgaacgtgtt gggaacggaa acaaaccctg
     3661 tgaccatgcg aggtttgaac atctcagccc ccagctcctg gaaaggtctg cggttctata
     3721 atcccgatgc ggtgtgcaac ctcaactacc tcaatgtcta taatgccggc ctggatgaag
     3781 gagacactcc cagtgaggat ttctgcgctc tatacatcta caacggcact gtaaacctca
     3841 ctggctgccg cgtcgccatc agcaatcaca acctgctgaa actggaaggc aacaacagca
     3901 ccactctgtc caactgcctg ctggacagcg ctgtaaacgg catcatccac aaccagggga
     3961 tcctgaacct ctacaacaac acgatttcag ccctcagcgg tacaggtatc ttccaaaatg
     4021 gcggcaccct caattttggc tccagtcccg gccagtggaa ccgcgtctat ggcaacacct
     4081 tgaatctgcg caacaacacc gccgccgttc tcaacgctcc ctatgtgtat tggggcagtt
     4141 tggaccccgc cgtgatcgat gccacaatct gggacaacga agaaggcagc ggtacggtca
     4201 attttgagcc ctggtatgac gtcaactgcc tgcagctcta ttataacacc ctcgatactc
     4261 ccaccgggat cacggtattg cagcagacgg ctaccagcct gaggctgagc tgggacgcgg
     4321 tgccggccgc cacatcctat aaggtgctga cagcccagga cccctatacc tcagagtgga
     4381 ccatcctgca gcaaaacatc agtggcaccc agattgacct tgacatcaat cccgccctgg
     4441 gaaaatgctt ctacaaggtg gtggcggtgc gctgaaacgg gtttgtgttt cccgcggatt
     4501 acgcagattg aggcgcggat tacgctgatg ttgtgatggg aagtgtgttt cccgctgatt
     4561 acgcagattg gaacgctgat ctcgctgatt taagacgatt gagaagatgg ggtcaaaatc
     4621 ctctaaaccc tgtcataatc tgcgtaatct gcgcataaat ctgcgtaatc tgcgggaaac
     4681 tgattatact gttgtaaagg aaatatgaat atgatacgcg gactgatctt tgatatggac
     4741 gggaccctgc tggacacgat tgaagatatt gccatagcgc tgaatcgagc tttactggac
     4801 atgggattga ccggtcacaa cctggaggag tgtaaaaccc tggtgggctc cgggattgaa
     4861 aacctggcca aagctgcgct tcctcctgac aggcataacg aagcgaccat agaggaactc
     4921 gtgctgagat tcagagctca ttacgcccaa aactggcatg aaagcaccca gccctatgag
     4981 ggtattgaat ctctgctgaa cacactggta aaaagaggtt tacacatggc catcctctcc
     5041 aacaaatccc acggcttcac ctctctgatg gcagaccact ggtttatccg tgatggccat
     5101 cagatcttcg atcccgtgtg gggagagcgc agcggagtgc cgatcaagcc agatcccagt
     5161 tcctctctgg ccatagcaga gctttggggc ctgaagccct ccgaaatagc ctttatcgga
     5221 gattcggaag cggatatgct cacagccgtc catgccgaca tgttacccat cggagtggcc
     5281 tggggcttca gaagcgtatc tcaactcatg gcctccggag caaaaatcat cctgcaaagc
     5341 ccggaggagc tgctgaagta tatgtaagcg ggatagagag ttacccagct cctgctcagt
     5401 tagaagctta agtcaacaat ttaccattgt gtcaagttcc cgcttagcct cctttacacc
     5461 acctatgcct ctcctttaca tctcctttgc ctcggcatag gagaggcaaa ggtgatgtga
     5521 gagggaggca gttgaggacg tttgccaatc ccatggctgc cagcaagatc aaaatctgat
     5581 ttgctcattg tctcattgtt caaaaccctc atattcctac cgcatccaaa acctcagcta
     5641 tttccaaagc aaacgaggac gtttgcggtt cctccagcaa agcgttccct gccactctct
     5701 tctgtgctca gcgctatctg tgatgaaatg gcaagatctg gctctccggg taaaaaaagg
     5761 aaaatgaggg ggaaaaaatt caaacccccc cccccccccg caaacaatta agcttgacat
     5821 ccgaatggca tcgggtaaaa tgtgatcagg gagaagctta tgaagcgata tctattggcc
     5881 gttttattgc tgactgcgag cttgagcctt ttcgctcagc aaataccgtt gcctctgctc
     5941 tgggaagacc ttacttacct gggtaggaca gactgcgtga tcgagggtga tctgatgtat
     6001 atgacaaact cattaaacgc ggtttccggc cccggattgg acctcgcgat tctggacctc
     6061 agcgcttccc aaagatttaa aatcctgggc actttggacc tggatgctcc agcggcctcg
     6121 cgtcttgccg tgcacgcgga ccgggcaatc gtgatccaca cggacggtcg gctgagcgcc
     6181 attgatatct cgaatccctc ccagcccctt tttataactt ccacgctgag tcccgccgcc
     6241 atcatagcct gggaaagcta tggccactat ctctatctgt atgacagcca gggatatatg
     6301 cgctgctatg cgatggacgc tctggacctg gtggcctcta gctggatcgg cacatactat
     6361 acagccatga ccatcgagtc tggaaggata tatttgagcg gcaccatgcc cggcttcaga
     6421 tgtctgggca ttgaccagcc ctccgcgatc acggatttgg gtactctggg gaacgcgtcc
     6481 aaccagcaaa tcatggagtc cgggagtggt gtgttgatct gctggactcc tggcagcgct
     6541 ttgcatttct atgacatatc ctctgtggga atttgcaacc tgatccagac ctggtcagct
     6601 ctgattgccg attatatcag gatcgacgcg tcagggggac ggatggctgt ttatgcccgg
     6661 ataacatatt cggaatacta cgagagtagc ctgaagatct gtgatctgga ggctgccgga
     6721 ggaccgcaag tcatctacac agacgacaat ccaggcacac ttagctggct gtgtatcagt
     6781 ccggactacc tgctgcacct ctatgaatac tttggcaacg gctacaccta cagcctctac
     6841 gatctcacga acccctccca acctcagtat ttggacacgc tgctgacagg tggggtcagc
     6901 tatctggcca ccaattccaa ccgcctgttt gccaacgtcg gcatgcaggg cttgaacatc
     6961 tatgcttgca gcgaaaccgg agctattgtg cagaccaggc ttccggagcc ctggatctca
     7021 tatgtggccg ccagcgatca gcatctactg atgaacagag tgtggtataa tggaccggga
     7081 ctgccaactt caaatgccat atacatttac acaatcctga atgacgatga gtatgagtgg
     7141 gtttactaca caggcagctg cagtcttggc gatcccatca ctgatgttct ctaccaggac
     7201 aaccgcttgt atatgtgcgg ctggggaggt ctggccattt atgatctgac ggatacctcc
     7261 catcctgatg agcttgcgcg gctgcctggc agctatttct acctgggggt ccaaggcgat
     7321 tatctctatc taaccgggta cgtaaacgga tccaagctgt ggatctatga catctcggca
     7381 ccgtcttcac cccagcagtt gagctggctt gcagctagct atctcgacct tggagatttg
     7441 caatatcaca ataacgtgct gtttgtgggg cggcaaccga tggtggtgga tgtgagcgat
     7501 cccagcaacc cggaggtcct gcatcaatat ggtacgatcg tgggaagcgc taacgcgtta
     7561 gaagtagctg acaacgcgct gatcgtgatg aggtggaacg atctgagggt ctttgacatc
     7621 agttctatcg caaaccctca gttggtatca tccacaagct ttgacaccga tgccaattgc
     7681 atgacggtga aggacggcct ggcctacata ggtcataaag aaggcctgag ctgctatgat
     7741 ctgagagacg cgctggccta ctgcgcagct ctgagtactc cagatgacaa taaccaggtg
     7801 gtaccagcgt tgtccgtgta tcccaatccc atgcgggaca gctcgataat gaaactcgag
     7861 cttcccgaag ctggcgatta cagtattggt atctacaacc tcaaagggca attgctgaag
     7921 cgcttcccca gccagactta tccggctggc agcaacactc ttacatggac aggactgaat
     7981 gatcaggggg tgaaacttcc ctccggagtg tatctgctga gggccagcag ccccagccgg
     8041 acgatccagg ccaaagtgat tctactgaag taatccaggc tcagagctgg atgtagctga
     8101 tctctaagct gtaagcccca tcccggagaa ccggaagctg ggccagaacg gaccggtagc
     8161 gttccagctg ctccgtttcg tagatcccac cggtcttata atcgatgatc agggcttgtt
     8221 tggcaagggt atccagcatg aggcgatcga cgcgcaactc tttggctccc tgatagatgg
     8281 gcagttcggt gaagactttg ttccagcggg gctcgaagag gagaggaaaa tctctcagcc
     8341 ggtctctgag ctttttcagt tcggcgtcga tgcgctgcag tgtcaagatg ctgcaataat
     8401 gtgatataac ctgtcggcgc gcatggctgt gctcctcggg aagatcgcgg ataatgaagc
     8461 tgaggtaata atgccagagg tcgccgatga ggttggcacg gtcttccagc cagatctgac
     8521 gacggtttgc ggctggatcg agctgttgag gctcagccag gggaaggggc gcaaaatcca
     8581 gagctgaggc cagcagggaa gcttcacagg agatcagcct gggagtatcc gattcttgct
     8641 tgccctgttt gtctggttcc ggggcgccag gctttggctg tcccagcttt tcccgctgac
     8701 cgtcaaacca cctatggcag gcctcgcaga gcaggttggg aagtttgtcg tctttggtat
     8761 cctgatcgtt cctgtaatcg ctccattctt tggtgctcct ataggtgaag aggatgtgca
     8821 gcttggtttt agcccgggtg aatgccacat agaggttgtt catctcttcc agcagggcgc
     8881 gttgttcctg gcgggtatgc aggtccgcgt agtcggaatg ttccaggagt tggccataat
     8941 gatagctgag ggcaaaatcg ctgatgctgt tgaagtcctt gccggcaaaa agggcatacc
     9001 agtccagctt tttgccttcg ttgccgtgcc ggccgctaag gttgtaaaac acaaagacgc
     9061 ggtcaaactg gagacccttg gatttgtgga tggtgagcag ttgcaccttg tcccctcctt
     9121 cgatggccac ttgtttgagg gcatcctggc cgcgcaggtc ttccagatat tccaagaagg
     9181 cgggcagatc tttgcgggtt tgggagggat ccagctcaaa gctggcggtc agagacaaaa
     9241 aggcatccag attgagctgg tcacgctcgc tgagcgagcc gcggtccaga tagcaatcta
     9301 ccagctgctg gcaaatggcg tacactccac tatccatggg cagctctgct tgggagaact
     9361 tgtgcaggag gggctggtcc ttgaagtcag gagctatcgg gggcttttgc tggcccgctt
     9421 tccagatggt atccaccgcc agcttgagag cctgaggcct gagcaggata taatcagacc
     9481 gcagccaggc cagccagtcc agccaatcct tccaggccag ccatttgaga tagctgagca
     9541 gcggcgcgat cagatgatgc tccggcagtt cggcattggg ttgatagacg ctggtgatgc
     9601 caaactcgtc catgagttgc tgcatctggc tgagctcaga gcctttgcgg cagaggatag
     9661 cgatagattc aggctttttg gctgagtagc cagcttcttc gtccgctttg cagtagggca
     9721 tcacccagtc tttgatccac tgcctgcgca cttcatccag ggagcgctgc acgttcatgg
     9781 tctgatactt ttcgcagtca aagctaagct gcgagagatg attgatttcc ggcacggcac
     9841 tggtaatggg ttcataatcc catttcatgc cccgctgagc cagataatcc ttcagaccat
     9901 tatcctggaa gatggaattg atgaaatcca tcagtagagg actggagcgc caggatttat
     9961 ccagcctgtc ctgacggatg tctttcaggg tgggaaagat ttgtttgagg ttcaggagca
    10021 gttcacgctc gccgccccgc cagccaaaga tggattgctt ttcatcgccc actacgatca
    10081 ggccgccgta atcctggctg ccgtggcccg cgcagacttc ctcgatgatg ggcttgagga
    10141 tggcaaactg gatcaatgag gtatcctgaa actcgtcgat gaggatatag cggctgcgat
    10201 gggagagaaa ttgatagaat tcattggcgg tgttctcttc cgagagctcg aaattgggcg
    10261 gttctccttt aaacagggcc tccagggtga accaggcgat gtcgttgtaa gtcatgttct
    10321 tatagcgata gatcaggtga tcgtaggagc ccaggatggc ctcccagatg ctgaaaatct
    10381 ctgcctgttc gggcaggaga tagctgtgga ccagatagtt ggcgagctgg cgacgtgctt
    10441 cctgcaggag ttcaggcaga tgggagtttt ccggtttatt ggcccggatt atattgccgt
    10501 tgtagagttt gagctctttg atggtcttgt agagggccat tgcggatgag gagcgctcca
    10561 ggatcgggtc cagctccgcc aaaaaggact ccggatcagg cggaaagctg gcaaagagtt
    10621 tgcgggtgtc cgcgttgaag agctcttccc agtccttttg cttggccagc gcgcgatcgc
    10681 tcacggtttg gaggatctgg tccagggcct ggaaaagtcc gtctttggcg gcctgagcat
    10741 aagcaggatc gggatggaga aagtgatatc tgagggtggc cttgccttct ggatttacag
    10801 gcctggtaag cagatagagc agccagcggt tgtcgatcag agatgtgaaa aactgctcat
    10861 aagcatccag ggaggggctc actttgcgcc tgagcaagga atcgaccacg gccctgcggc
    10921 tgggcgtcat cagatcgctg agcaggaagg gcatccgctt tttcacggcc tgcaaatcca
    10981 gctcaaaatc gcctatgctg cgcaggggct tgacgatgtt gcgaaaaatg gaatggatat
    11041 aggaatccag cgtcatcacc tgcagcattc ttctgtcagc tgccaggacc atgctgacgc
    11101 tggtgaggat gccctcctcg ctggggctga gctctccatc cccctgccgg tatttgagtt
    11161 ccaggatcaa ttcacgccgt tcatcgcgat cctgggaatg cgtggaaacc agcagcctga
    11221 ggtggtcctg gatgcgttgt ttgatctcag cggtggcctt gcgggtgaag gtgagcacca
    11281 gaatgctgtc catgctgaag ccgggcagat caaagtggtc cagcaggagg cgaacatatt
    11341 ccagggcaag acgatatgtc ttgccagtac cagcgctggc ggtgatgatc agaggtttga
    11401 agcgcgtcat gattccctcc ctgagggtgc cttccaaaga tcagcccggg tgatctgctt
    11461 gaggcgttcc cgatcagtgg ctttggtccc gaggccataa ccggagctca aacactggtc
    11521 cagaatctgg atgaggccat cccgccagga ctggcgtttt ttgtcgctga ggttttcact
    11581 gtcgcgccgc gcgtccagaa tcatccagaa gagagaattg agttgatttt gatactccgg
    11641 gtccaggcga taataaaagt attcataaaa gatcagctgt tccgcgtctc ccttgccggt
    11701 cttgaaatcc acgataaagt cctggcctgg caagctgatt ctcaggtctg ccttgccgtg
    11761 gatttccagt ttatactctc cctgaggccc aaagctcagc agggttttgt attgccattc
    11821 ttcattcgtc atcctttcct gctcagggat caggcgaaag ctataaccat agaggttggg
    11881 ctccagccac tgataataaa agtctctcac actgcgcacc aggcaatctg agatcacgcc
    11941 aaagaggaag tcgcggttgt agttttgggg cagtttgtga tcaaactggg ccgaacccaa
    12001 tagcttccgc agcttctttg agaggctttc cgggctctga aaggcagctc tcagacgccg
    12061 cgtatcctga tgctggcctt ggagttccag cagaatatcg gtcaaaaagc tatgcatgat
    12121 ggtgccaaac agcatgggcg aaatggtctc ccgcggtaga agctccaccg ctttgagacc
    12181 acggaggttt tggatatacc aggcaaaggg attggcggcc agagcgctga gaccgtagta
    12241 agcgcttttg atgccagtgc caaagtcctg ctctggctgg cagggcaggc taaagaaatc
    12301 ctccgttggg cttgatcctg gaagaccttg cgcctcaaga tcactaaagc ggggaccggg
    12361 atgcaagata gccctggcgc gggccagcag atcgtgggcc aggaccgttt cggatctgac
    12421 gcatcgcagc tccggccagg cttgcgagct caggtgtccc tccagttcgc tgagatagga
    12481 gccggggtcc aggtcccgct cttcatctct gtagcagtac agggtggtgg tttgagagcc
    12541 cagcacgagg cgtaaaaagt agtaacgttc ccattgcctc accagctcaa aatgtttgag
    12601 ccccagtttt ttacgctgga cttcgttgag cagccacacg gcttccggat tggagggcag
    12661 gatgccttcc acacagttga ggacgattac ctcgtcatac tggaggttgc ggctgtccat
    12721 aaggttggcg atatcgtaaa ggggcgcgga aggggaggca tattttgaaa cgcttgcacc
    12781 cgccaggtga tccatccaaa gcttccagag gcctgaggcc agagcggtgc cgctagcgtt
    12841 gaagaagagc ttccagtccg ccacgagacc gagttcttct atccctccaa agttggccag
    12901 tcgttcatag aatctatcca gaatatcggt gctggccagt tcttcaggac tgcagaggcg
    12961 cttcagatcg aggccgttgg gggtatcaaa gcaagcgctc acggcttcca gggacgccag
    13021 ctgcgagagc cggtccagga gcgcgaaatg cttgcccagc agatcgataa gcagcttgta
    13081 gcgctcgtct tgaggatcgg cggcaaagag attcagatcg aggtcgatat agagcaccgc
    13141 ttccatcgcc agccgtctaa gctggtcaag cgcttggtgg cgatcggttt cagtccagtg
    13201 gggcagataa tactggcaaa aacctggaat cgcgagcgct ctgctcatct cagccagggg
    13261 aagaaaacgt tggttttggg ctgctgccag ggccttgaga tgagtggcgt acacgcttag
    13321 caactgatag agacgggtgg aatgcatgga gagagcatga ctgaaacgaa aacgctgggg
    13381 atcaaaccag cggctatagg acttggaagc gaagccgccg tcgacaataa cgcgccgggt
    13441 ggaagtttgg ggagccgcaa gcgtctccgc gtgggagttg agctcacaca ggcaggccaa
    13501 tgccatctgg tcctgatccg gagcctggat gatcttcagt tcccgcgggc gaatatcctg
    13561 ctggtccaat gcactgagct ccagaggctt gctttccagc ttgagaggat caaagctgcc
    13621 ctgggggccc tgatgtaaaa acactatctc acagccgtag gcttccagcc tttccaccag
    13681 gagcttttcc agcttgctga agtagtattg attgacaaac accagcttgc gagcgctgaa
    13741 ggggatcaca gcctgctcca ggcccaggta gaagatgctg tccgtgaagc ctttagcaaa
    13801 tataaagtca tgatagcgct gccggatggc cagcacgcgt ttaaggtgct cctcctgcca
    13861 gagtctggtg cccaggtcca tttgggcagg atcttgcagc agctcaagat ctaccagttc
    13921 gtcccgcatt tcggcaaaga aactcaggaa gctctggccc cagctcacga gatcgtcata
    13981 gcagtagagg tggaaggtct ccttatccgt ttcatccaac acctgccaga gacagagcag
    14041 gcgtttttcg tcctgcagtt ctggatgggg ggctggcaac agcatctggc gcagctgctc
    14101 catgctgata aaatcgactt cctcaagctg ccagcgaggc tggaacagcc ttctggcctg
    14161 actggcagac gcgagggtgg gaaagatcaa caggctggga tcaactatct ctttgatagc
    14221 cgccgcgttg agatcggcag aaaaaggcag gcagacaaga ctgcgcatag cacctcctga
    14281 gcgttagaga acgggtgaaa agagccggca gacggattcc atcgtgcggg aaaagatatt
    14341 gcggtgctgg agtttgacca gatcgacctc gcgactcatt agcagatcgg cctcaaactg
    14401 ctccagcgcc ttggccaccg tggcgggatg atacaccatg gccgttactt caaaattgtg
    14461 gttgaaactc cgcaagtcca tattggcggt gccgatggag agcacttcat cgtccacgat
    14521 cagcaccttg gagtgcagga agccgttctg atattcatag atcctcaccc ccgcttccat
    14581 caattcgcga tagtaggacc gcccgcccca ccagacgatg aggtgatcgg gcttaccggg
    14641 caggatgatt tggactctta ctccgctcat ggcagcagtc ttgatcgcca tcaagaggct
    14701 ttcattgagg atcagatagg gagacacgat gcgtatacgc ttgcgggcgt tggtgatagc
    14761 ggcaaaatat gcctgcatga tgctggagtg atcggtatcc ggaccgctgg ccgcgatctg
    14821 gagagggatg tcttccagac tggagggatc agtctgagcg ggtggaacgt atttgggaaa
    14881 gagctgacgg gtgagtaggt tttgcttgct gacaaaaaac caatcggtga ggaagatggc
    14941 ctgcagggtt acggccccct cgccttcgat gctcaccacg gaatcgcgcc agtaaccgta
    15001 atagcggttt tggcccaggt atttattgcc gatattgagc cctcccagat aggccttctg
    15061 gccatccacg atcacgatct tgcggtggtt gcgatagttg gcgcggctgt tcaggaaggg
    15121 gatccacacc ggcataaaag gcacaaacag cactcccgct gccctgagtt ccttcttaaa
    15181 ggagggcctc aggtcccaac agcccacatc atcatagata aacctcactt ccactccctg
    15241 gccggccttt ttgatcagga ggtctttaac gcgcttgcca gtctggtcgg aagcgatgga
    15301 aaagtattcc agatggacgt gatggcgggc cttttcgata tcggcgcaga tctgatccag
    15361 ggccgcgttg gtgtctggaa tgatttccac ccggttgcga ctggtgagga tggccttgct
    15421 gttggaatcc agcagatcga ccagacgact ggccagagca ggcaaatctc tgcgatagct
    15481 ttcaccctcg gaaggccaga gatcgtgcag gttgatggcg tcggccaggc ctttgcgatt
    15541 gaacagccgg gttttacgcc agttgcggcc aaagaagaga taaaagatga aacccacgac
    15601 cgggaggtag agcaggatca aaatccaggc cagagtgtgg acgggattgt cgttttccag
    15661 gaccaatacc acgatcatca gaaggatggt gacgccaaag atgatgttta gcagcttttg
    15721 ggccaggttg ctgaacacca gaggaaattc acctccggtc agccactgca gcagggaagc
    15781 cgtcagcagg atcgagatca agaggatcat gatcaaaagc acactgtccc aaatcttctg
    15841 agaaaccacc atatctcctg tgatagtatc tatttatcct atcgcaccga tggcgatagg
    15901 cttgagaacc atgtttccta aagcggccta aagctgtcaa tcagattctg gaaatcgggt
    15961 gcgagacctg ggtagtattt cccgcagatt tcgcagattt acacgcggat cgctgatttc
    16021 cggtagttga gaggttgaag tgatttacgt tgctggaagc ctggaccctt taactctata
    16081 agaatctgcg atatccgcgc tcctatctgc gtaatctgcg ggaaacccgt cccaagctca
    16141 tttgatgatc acgaacttgc ccactttttt tcgtccgtcg gcccggtttt ccacgctgaa
    16201 gagatagact cccggagcga tgatctgccg atgcctggaa agcaggtccc aggcatgcat
    16261 gccactggca gagaggcctt gggaagcggc tttggagacg gtgaggatat ccgcttcata
    16321 ggctccctcg tgatgaatct cttttaccag gtctccggcc agggtataaa tcctgatcac
    16381 gcagcgttct ggcaggttta caaaccagag acggcggctc ttgtctccct tctcgtcgtt
    16441 ttcgcggcgg ccatcaaact tgctgcgtcc caaataggga tttgggatca catacacgtc
    16501 gtccaggttt tgtctggcaa gggtgccggg aaagaagatc agcatgttgg catcggcatc
    16561 gcgaccggtt tccagagcgt tgagctcgtg cgaagggatg ccgcggtcat aagcactgag
    16621 ggccacgtag tattcgatcc cgcggcgggg atgatctatg gaggcgcgat aaaagcgccg
    16681 ggccaaacgc ttgtccttca gatcctgaag ggcttcaagg ctgccaccca gatggggaat
    16741 agccacctga cctccatgac ctggcagggg gatcatcttg ctgtcataga gtgccttata
    16801 gatatgttcc ggcatctggg gatgcctgaa gatatcggcc tgcagatctt tgatcatatc
    16861 atcgggaagc ccggagtggt cctccgcgat ctgaaccgcc tgggcttcca gagcttcact
    16921 ccagtccacg gcctgatcat agatcggaaa gccataaaag ctttccgcgg gattggcggc
    16981 aaaggcataa ttgatatcca gacacgtata atcctgatat tgggagctct ctggccaggc
    17041 gttgtggttt ggcagccctt tgtcgatccc cagatagcca ccgtaatcca caaacagcga
    17101 atccgcgtag gggctgtaga tgtttaccct gtagtcctcc aggtcttgat aagtatcctc
    17161 tttatcccag cgctcgatca gcacccaatc ttcctgagag ccgcttccac tgcgacccca
    17221 gaggctgtaa ccctgaaagt cgtgccttaa gcggttggcg gtgaagggat tgacggtggc
    17281 attgggattg ggagcggacg ccgcaggctg aaattctgcc ggatatccgc tccagtctat
    17341 aaagagcggg tcgctgtcca gacccggaag atactgaggc ccagagggat cctgccatcc
    17401 aatgataccg ctgcccacgg ttttgacgtc gtaggcaaac tcgctgcggt tatcccacca
    17461 gaggtctatc cgcttgccgt catcctggag ctctgcatag agcgctggga tgggaggcgg
    17521 ttccggagga ttgtagtgag gaaaggtatc cggcaacacg acgttgacca gcgtctgggc
    17581 cctgccgtaa atctctttgg cccagcgagc gctgcccttc agatcttctt tatcttcacc
    17641 tggaaacacg gccaccacga tcttcatggt gcgcccgggg gccagattcc agcgtttgtg
    17701 atagacccca tccagcattt cgttgtaatc ggccgtccct ggttgagcgc cataaaaaga
    17761 aaagagaaat ctggtgtcgt tgggagacgg ttgcacgtat tctgtgaggt catcctgctc
    17821 cggtctgaga ggttgaaacc tggcttcatc tggatttcgg cctgtgagca gccagtactt
    17881 ttcgttggaa gtacgcaagg gggcatagtt gaaattccag ggatcaaagt cactgggacc
    17941 atcgtcaccc gcccaatacc agcaatggaa tttcagcagt tccggatcgg gggtgcagac
    18001 ccgggccccg atcattccgg acgtgagtcc cccgtcctta tcggcgtcgt aagtataggc
    18061 aaattcgtaa cctgtgcctt ttacatagcc agacatatcg tccgcgtaga tggcttccca
    18121 ggtctggggc ccgcagtcgg catccatata aatgcccatg ctgcagtcaa acagcgtgtc
    18181 ccctgtggcg atattcatgt tggtgatatc aaattccaca tagacaaggt ttttgatata
    18241 gtcatagctc cactggtagc tcatctggcg agcccggatg ttgaggggat gatgacggtt
    18301 cagagaacgg tgggccccca aatcacgatc tcctgccgtg ccaaagggac agtagtcgta
    18361 gtagtgcgat acatagtcct gttcagacac cggagcgccg tcctcatcga agcgtccgtc
    18421 cgtgtcatcg tcataaggag agttgaacgc gtagctggca aaggagcggt cggcaaggtc
    18481 cataaagccc agtggaaacc agatttcgga gttgctgccc tcggtgatga tggcatagtt
    18541 gttggtttca tggatataat gagacccaaa cagcgagaac tgggcaggaa gctcagtgcc
    18601 atggcgcagc gggaaggtgt agcccaaaaa gtcctcgtcg atggtgccgt ccccgtcgtc
    18661 gtcttcagcc cgcttttgtt ttctggtgct ggcggtggca atggcgtcca cggtgttcca
    18721 gctttcatag aggtccgcgg catcgggatt gccggccacg agaggattgt aggcaggcag
    18781 catctcatag actccccatt cgccatcaaa acccagggtc accagggtgt cgatcaccgg
    18841 tttcatccag ggctgccatt cggaggtccc ttccgctacg gtcccagtgc tgtccgcgga
    18901 agggtttatg gcacgccagt agagcttgcg gcccgcgccg tcgcgccggt attttttggc
    18961 accaaaccac agcgcgcctc tgaaaaggta gtcaattccg ctgccacctg gatactcgag
    19021 ggaggggtat ttgggattgg catctgtgcc ggagccaaaa aagccgtagt tgctgactct
    19081 cagccagata ttgcccacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
    19201 gtgccggagc caaaaaagcc gtagttgctg actctcagcc agatattgcc cacgttgtga
    19261 accttgctga ggtccagacg ttttgtcccc atgctctgag gttcctggct cacggccatc
    19321 accagcgtcc agcccaaaag caaccccacc agcaagatac agcgtttaca tctcatgact
    19381 acctcaacat atttatcatt ttgtgtccaa gccccacagc gccagctctc acatggcgaa
    19441 gtcatctcac ttgataaact tcccctcttc cagttccttg aaggccagcc gcagttcttc
    19501 tttggtattc atgacgatgg ggcctcccca ggcgactggt tcacccagag gttttccact
    19561 caggaagagg aaatgaccgc ccttgtgagt ggcttcgatc ctcaccacgt ctccatcggt
    19621 cagcagggcc acctgatggt cgctgacgat cttggggccg attttaatgc caccttcata
    19681 gcaatagaca aagcaggtat agctgggatc tacaggcagt tccagcacat cggatgccgg
    19741 catgcggatg atgtagtagt gggggtccgt gacgatgtct tgcactgggc cccgggtgcc
    19801 aaagacttca ccgctgatca ctttgatatg gctggcgctg cttttaatct caggaatttc
    19861 cgctgccagc acatcacgat agcggggcgg catcatcttc tggctggcag gcagattggt
    19921 ccagagctgg aagccaaaca tgcggccttt ctcatccggt ttgggcattt cctgatggat
    19981 gattccgctg ccggccgtca tccattgaac gtctccggga ccgatcacgc cgctgttacc
    20041 aatgctgtct ccgtgttcgg cagtgccggt cttcatgtat gtgatggtct cgatgccgcg
    20101 atgtgggtgc cagggaaacc cgggtagata gtctttggga tcgttgctac cgaagtgatc
    20161 gagcatcaga aagggatcga actggttgat ctgatgatag ccaaagacgc ggtagaggcg
    20221 tactccggcc ccatcggtac tgggctgacc ggtgaggacc agtttgataa tgcgggcttg
    20281 ggacatgggg gtctccttga ttggatcaga aagtgacgcg ccggctaaag ccaacgctgt
    20341 ggatggatta tattttcagt ctggaacggt tcttttccag agtggcgggc ccgatgcctt
    20401 tcaccttggt gatgtcctcg atgctggcaa aaggaccgtt ggcctcccgg aactcgatga
    20461 tggccttagc tttagccgga ccgataccgc tgagggtgca gagctcatcc aggctggcgg
    20521 tgttgaggtt gacgatattg gtgagatcgg ctttcttggg ctgggcctcc gctttggaag
    20581 cagggctatc tgacttcgtg gcaggacttt tagcgttttt atcgatgggc tcggcattgc
    20641 caaacaccaa cagcataggc cgcatcttgg cataagtttt caggccgatg cccttgatca
    20701 gcatgatctc gttgacgttg ttgaaaggcg ttttagcgcg gtgggcaata atctcctggg
    20761 cccgctttgc tcctatgccg ggcagctctt ccagttccgc ttgagtggcc acccggatgt
    20821 ctataatgat ggggacgtct tctttgagtg cggtggcaag gctttcggga ggcgcgtctt
    20881 ccaggctctg attggccagg agcggactgg agggcttcca gcccagcagg ttgagccccg
    20941 ttccgatcat gaaacagagc gccagaaaga gcagggcctt ttgctcgctg ggtgtgagga
    21001 tctcattgag gggattgcgc atagctgcct ccttcagtgg atgggatacc tttcccacca
    21061 gaagcgaaga tcggtcccat cgtttatata gtccagcagg ccgtccgggc caatttcgat
    21121 cacggcaggc agatacttga tccccagccg ctccagactg gcatcgtcaa ttcttaagta
    21181 aggcatctcc cacccaagct ccatttgttg ggcttgggcc cgaattggat cggagtcgta
    21241 accgttcacc aataaggtga ccaccccgga ggagtcgcta acggactctt ccagatacac
    21301 ttggagctga cccctctctc tctggttcca ggacgcccag aagatcaggt gcagcgtctg
    21361 gccaagataa tcgctcagct tcagaggcac cgcgttcgcg tcctgaatat ccagctctgg
    21421 aagctgcagt cttaggcgct gctgttcaaa ttcctctttt tccctttggc gggccagctc
    21481 ttcatactca ggaacgcggt cctcaaattc atcccagcgg gcgtcctcaa aatcggggtc
    21541 cagatcgctg taaaccccca tggaat
//
LOCUS       DAOSFN010000022        21393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_1989_length_21393_cov_9.333771, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000022 DAOSFN010000000
VERSION     DAOSFN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 21393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21393
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_21393_cov_9.333771"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="PLO35_02740"
     CDS             complement(<1..1217)
                     /locus_tag="PLO35_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOH78611.1"
                     /translation="MAVFKPFKAVRPLPEYAGAIASLPYDVMDSDEARAEVAKNPLSY
                     IHVEKPEVDLPLGTDLYDPKVYAKARENLESYVKRGMMKQDDKPMFYIYRQVMDGRPQ
                     VGLVGLASVDEYMDGTIKKHELTRADKEADRIKHVDVCDAHASPVFFTYRHQAGIDAI
                     VNKVMDNHAPVYDFVSDDGIGHSLWLMDDPADIAAIQNAFAKLRYLYVADGHHRTASA
                     AKVGLLRREQHPDYSGHEEFNHFMTVIFPDNQLKIFDYNRVVKDLHGLSKEDFLDRVS
                     VDFDVTAMYVNGPYHPEKTHEIGMYLSHTWFRIVPRKGTWNASNVVDSLDVSILQSNL
                     LAPILGIGDPRRDKRIDFIGGIRGLEELERRVDSGRDAVAFAMYPTSMDELIGIADAG
                     EIMPPKSTWFEPKL"
     gene            complement(1236..2507)
                     /locus_tag="PLO35_02745"
     CDS             complement(1236..2507)
                     /locus_tag="PLO35_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOH78612.1"
                     /translation="MNNHIQDLLSFLDDSPTAFQASLGIETRLQMAGFRALDETQNWK
                     LEKGAKYYMKRADTAILAFIIGSKPLVESGYQIAASHIDSPSLKLKPATVKTESCVTR
                     VGVEVYGGPIISTWIDRELGIAGRVTVKTGDTYRSIPVNMNRPLAIIPNAAIHLNRDI
                     NKGFEYNKQVHLQAILATGSEAGNPLLSALAAHLDLSLDSIREMELYLYDLAKSSLTG
                     MDASLIVSGRLDNLAMTHAILSALTEAQTPQATCLAAFFDHEEIGSGTQQGADSSLLG
                     EVLERIALALGLKREEQLRALHSSFLISADMAHAFHPSYAEKYDRDYAPAMNQGPVIK
                     INGNYRYTSTSESTLRFLDLCEAAGIKAQKFMTRSDMPCGSTVGPVVSALLGIPAVDI
                     GNPMWAMHSARETCGVKDHKSLIKVLKQYYI"
     gene            complement(2670..4310)
                     /locus_tag="PLO35_02750"
     CDS             complement(2670..4310)
                     /locus_tag="PLO35_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH78613.1"
                     /translation="MIQEKLIPYLIDSIKNYWDMPAFTDYPGPALKYSDVGKQLLWIH
                     RLFKACGMKKSGKVALAGKNSGNWATLWISTVTYGGSIVPILANFSPEDTQHIVNHCE
                     AEILFVSKDKYDSIDPDQLRKVKYIFSLDDFSLLFARDETIKKIIKPISETTDLKGLS
                     AGGMNVEDGCDNEDIGAIIYTSGTTGFSKGVMLSHRSLLANIIVARENLLFKEGAKVV
                     AFLPLAHAYACSFDLLYPFTRGNHINFIDKIPAPKVLLGAMQDLKPNVVLAVPLLIEK
                     IFKRQLQPVIETPKMKAMLKLPLLNKLVCKKIRDKLVTAFGGELTELVVGGAPMNAEV
                     ELFMKRIKFPITIGYGMTECGPLISYARWFEHRFESSGKLVKYIKCKIESSDPAKIPG
                     EVLISGEQVFSGYYKNEEATSETLKDGWLYTGDIGTLDKDSFIYLRGRSKNVVLGPSG
                     ENIYPELVEQKLNNLPYVMETLVLERDRKLHALIYPDLESLDKDHIPEARVPQIMEEN
                     RQEVNKVLADFSRISKVQIVNEPFQKTPTQKIKRYLYR"
     gene            complement(4739..6265)
                     /locus_tag="PLO35_02755"
     CDS             complement(4739..6265)
                     /locus_tag="PLO35_02755"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOH78614.1"
                     /translation="MTLWKRIVPLALILVLFSCANAQEEPTSFQAPVLQARSAAPEMI
                     QPAQNPVVEVVRRVREAVVQIRVEAKITVQNPMNQFFDDPFFRYFFPEQPRAQQRPVT
                     SMGSGFVYDYNPQTREAYIMTNNHVVEQGKSGTITVTLADKVNYNAEVVGLDPNTDVA
                     VIKITVKEGEKITVAPLGSSSNLEIGEWAIAIGNPFGEAGLDRTVTLGVISAIGRSNL
                     NFGAGSPIYQDYIQTDAAINPGNSGGPLLNIYGEVIGINAAITSTSGGNIGIGFAIPI
                     NLAKRVVDDLVATGKVSRAYIGISPQEITSDLMEAFDLKEVSGVLVAKVEDDSPASKA
                     GIQTGDVIVEFNREKVPNVAKFRIAVATSKAGSIVPVLIIRDNQELSLNIELDYFPED
                     KAANGEIAPEVKLSHGITVDAMGSTAAQRLNISGTEGVVITAVEAGSPAAKAGLSVGL
                     VILRVNNLPVNNPDEFRLTMDGQIDAMSKSGKSTIRLYVKDRNNMFQYVALRFEMPKE
                     "
     gene            6674..8773
                     /gene="recG"
                     /locus_tag="PLO35_02760"
     CDS             6674..8773
                     /gene="recG"
                     /locus_tag="PLO35_02760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOH78615.1"
                     /translation="MEKTKPEFTRDTNTEIKFLKGVGDARGRILAKLGIRRVLDLMEH
                     FPRYYISRSIDPTLHEAKVGDHVAFTANVSWVDTRTTKNGKKILNVGINDVMNTLTCS
                     WFSYPNWYEGILVPGKRIWVNGVISEFNGQLQMLHPEIEAVDDDTEDDFWKTRLCLPV
                     YPATANLNQKNLRRLVWQAFHDYASTIVENLPTYLIDKYQFKDRKTALQLMHFSQNPD
                     LPVQVMTRFAYEEFFFAQLLWARHRKYHTDEVKGIAFENQRRLTSALKNKLPFELTKA
                     QKRVIREIFDDMCSPRQMSRLMQGDVGSGKTVVTLFAMLLAVENGYQAALMAPTEILA
                     EQHFGTITSLLEGMDVRITLLKGGSYKGKAQTVTDIAEGAYDLIIGTHALIQDKVTYK
                     NLGFVAVDEQHRFGVQQRAALARKDKHPDLLYLSATPIPRSLSMTVYGDLEVSVIDEL
                     PPTRKPVLTLIRSDRKIELVWADVKKELAKGRQVYVVCPLVEESDKVDLLDAQRLHQH
                     LSSKIFKGISCELLHGRMPARQKDEIMKRFKAAEIMILVSTTVIEVGVDVPNATVMVV
                     EHAERFGLAQLHQLRGRVGRGAEQSCCYLIEHQPCSKIAWERLDTMARTTDGFLIAEK
                     DLELRGPGEFFGTEQAGLPNFKFANLIRDQHILKLARNDAFEIITADPDLQAEQHQVL
                     KKILTTELADKEKLILY"
     gene            8855..12133
                     /locus_tag="PLO35_02765"
     CDS             8855..12133
                     /locus_tag="PLO35_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOH78616.1"
                     /translation="MKKLTLMICLIIAISAVFAVKFDPYYFQSKTIITCFSRAAIGND
                     AGTIEFTKQDGVVRTGISSFDALASQYKIVDMKQMHPYVKVPEWNDKGAYLQNHYRVY
                     LESDDNIDAAVAALAKDPNVIYAELEGINRSKFVPNDPMLTSQYVHARIRSYDAWDYI
                     QGSYDVKVAITDSGVKWNHPDLRANIWVNPAESPGMTINWDAGTITGGNGSDAGEGGS
                     KVDDLVGWDFYNNDNNPIQTYAANDHGTHVAGCAAAVGNNAIGVVGTSPIASIISCKG
                     ASNTSPSTGISYAYDQVKYAGEIGADIINASWGGPGTGAYPNSIVNYVTALGALVVTA
                     AGNENTEHTASYQDYPADCTNVLCVASTGQNDVKSSFSDYGTTVDISAPGEGILSTIV
                     ASNGYDSYDGTSMASPNVAGVAALVKALHPTLTPAQLMQRLMMTADYIYEANPSYVDK
                     LGAGRVNAFTATMFDKIPYITIDDTMVEEQTGDGDGIPNAGETIRLKVSINNLLDPFT
                     GLAWQPATNLQTTLTTTYPGITIVNDSANYGTLSAGSTMWNNSQPFIFQTVANLPSEP
                     IPFELVASANQGSAFPYNATIPFTVRLSNNQAGWPVLIGGATNSSPIIVNLDADPQRE
                     MVFSDHLGNIHALNPNGTQMPGFPMALGSSVMGSIAMSDIDSNGQKEFAASLQNNNLA
                     LFNQSGQVIFNIPAGGTLRSGPVITSLAQNSSRQIVAAIQSGNIVVLDTNGNAYPNFP
                     AGVGGAILGSPAIADLNNDGYQEILVATLNGNLHAINSQTGQNIAGFPITMQGGSQNP
                     ITIANLDADNYPEIIVSTSTAGYIHAYNHDGSVFFQKNVGGSIKTGAVVADVNSDGNK
                     EIVVISNAGMVYILNANGTDLAGTPINVNQTVDCTPVVAKFDGDNYAGIIFGDTNGKL
                     HSVRANGTQSPNFPITIGGNIKISAALSDIDLDNDMDIVIPSDTGFYVLDIKRPAQSY
                     EWPCFMGSYNRGGNVYQATPSDDPVVPAFADALIGNYPNPFNPSTTISFSLAQSGPVS
                     LEIFNQKGQKVRDLSSGNLPAGEHQLVWNGKDDSGRPVSSGVYYFRMKSGKFSSSRKM
                     VMMK"
     gene            12313..12744
                     /locus_tag="PLO35_02770"
     CDS             12313..12744
                     /locus_tag="PLO35_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78617.1"
                     /translation="MKKIMILLILIVASTAAFAQTGLFDLSFGDSFEKCESLLKSKGF
                     SNNIGDFGPNYFEPDDLGYVAYIELNLDDTRHLKGWTVVYTGEVHDDYEEVAMDAIIS
                     WHGEDYDVDWDDWDFEYYEWELENGRYVEAVWDEYFYVYYY"
     gene            12939..13394
                     /locus_tag="PLO35_02775"
     CDS             12939..13394
                     /locus_tag="PLO35_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78618.1"
                     /translation="MYKPIVLLICVSCCVGILNAQGGLYGLDWGASHEAASLILIDQG
                     FKIQAHTPEQIIMVPLDEEPIKGIELRFDGQKDRLAGWSIGYSFLEEEDMGELVIGAL
                     ISRHGPDFEKTTEGIYLWQLGNGQTVRAGWDYFKAMFVVDYRQESLEAR"
     gene            complement(13399..14193)
                     /locus_tag="PLO35_02780"
     CDS             complement(13399..14193)
                     /locus_tag="PLO35_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOH78619.1"
                     /translation="MHIPVDKPILLALLGTLFTWGMTALGASFVLFFKSIRQSVLDTM
                     LGFAAGVMIAASVWGLINPAIDMSNGSVWPAVIGFLVGGAFLRVIDLLLPHVHESHGQ
                     IEKEGLKTPWGKSTLMFLAVTLHNFPEGLAVGVAFGALAYGGEAATLSAALALALGIG
                     IQNLPEGAALSIPLRREGMSRWRSFFWGQFSGIVEPLGGVLGAALAMSAKPILPYTLA
                     FAAGAMIYVVVEELIPEAQGGKHGHLATAGLMLGFCVMMVLDVILG"
     gene            complement(14222..14422)
                     /locus_tag="PLO35_02785"
     CDS             complement(14222..14422)
                     /locus_tag="PLO35_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78620.1"
                     /translation="MKSIFTYTDEHLGKMYIVIPKIREISQALGNLVVTFDNGDKRAI
                     DVPDTKAAMTQILEAIESFYNR"
     gene            complement(14430..14873)
                     /locus_tag="PLO35_02790"
     CDS             complement(14430..14873)
                     /locus_tag="PLO35_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOH78621.1"
                     /translation="MARASESALMMEILAWIRQNQYVYLATVDKKQPRVRPIVMYGYD
                     DRYFFTTFSGDSKVGQINANKWVEVCVPIAEDGQNGYLRLSGTASVVQNASLKAEAAE
                     HCYFFDQFFGGYDDPDYTLIEFDPEYVEYLRPGENHSQSCSFKRY"
     gene            complement(14886..>15254)
                     /locus_tag="PLO35_02795"
     CDS             complement(14886..>15254)
                     /locus_tag="PLO35_02795"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH78622.1"
                     /translation="EIFEVVKQVQNTQSGAAIIGKTVEWFQSRTGINDPLYGQILASP
                     VLYSYIAMANSGPNTNGSQFFIVTRKPGTPHLDGKHTVFGRVTQGMDVVHKIENLPRD
                     RADNPNLENQAFINSIVLPK"
     assembly_gap    15255..15354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15393..16130)
                     /locus_tag="PLO35_02800"
     CDS             complement(15393..16130)
                     /locus_tag="PLO35_02800"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOH78623.1"
                     /translation="MISDVHYKYRQESPADRENSQLFLSFLEGIAGRYDLLVLNGDIF
                     DLYNDWKYTIVRQYFPVLHRLANILEAGCRIVYISGNHDFWFNGFLDKELGFELYDDH
                     YSLRADGQKLLFTHGDLYTVNDLRYKLFRRLIRTPLVRLSFDLLHPDLALTIGAAMSR
                     SSRLRRMSDVLKRRKGNGLKTWARAQISKGKADIVIMGHNHEPEIISVGSGVYANAGD
                     WLRNHSYLEILDGKIYLNHYKHKEKAE"
     gene            complement(16144..16827)
                     /locus_tag="PLO35_02805"
     CDS             complement(16144..16827)
                     /locus_tag="PLO35_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOH78624.1"
                     /translation="MSRVKDIELILILTIMLAAASLAAVTGSITRAGFARLQYEGGGD
                     WYNDPEVLPNLARFVNATLGTTFPIDQTVVKAADARLFDCPFVYLTGHGNIRFTDREV
                     ENLRQWMQRGGFLYADDDYGMDQSFRREIKRIFPEHSLVELNPAYPLFRSFFDFSSGL
                     PKIHEHDAKPPQAFGVFDDTGRLMCLYTYESNISDGWADPGTHNNPPETRETALRFGV
                     NIVYHLITR"
     gene            complement(16800..18209)
                     /locus_tag="PLO35_02810"
     CDS             complement(16800..18209)
                     /locus_tag="PLO35_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOH78625.1"
                     /translation="MITLRSKAQNPPQAACEYQVDKDGRYGWFLCTWHPESHLILEEI
                     QAEIKLELMNSDIRQVSKLEAEKWLKSFFADFHWKLHARLRRTELKEKGISLFLGLMY
                     DNEIIFTQFGRMFCTLGGPKKTEAIGKDWEHSHLQTLEQLNLLGLSEEEIRVKTQKVL
                     VPERHFLLVLPGAMARELFGGHFDGGSVNALLDSLAGKPGALWLLLGNEPEHIKTKRK
                     GLSRLHLATILLIFFTLLAIVYVIFGGRFFEMTWRKARMLFQSKKPIPLEQIPNYLNI
                     DNQKVRNYLQQALKSPAREVDLKLIWNTDLPYQVTAAPGFDLDNIYLASNDMLLAFNK
                     KGRNLLWNKSLGSKVQGITGITGGLMINLEDQRVLGLNNNGELSWEQPLGSQVNIFHS
                     LKPMELTNIHDPRIDGSLIVVPTQRSILVLDGNSGEKLSEITFKDELQYLSTYDSFQN
                     CFYAVVQDTMICLELKILS"
     gene            complement(18211..19293)
                     /locus_tag="PLO35_02815"
     CDS             complement(18211..19293)
                     /locus_tag="PLO35_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH78626.1"
                     /translation="MIWLMFRMLGYWFMKHLGFPKLLPINYTVSLLYTCNSRCGTCNI
                     WKKKAQNLTVDEYRKIFKGIGRTPYWVTFSGGEPFLRNDIDEVVSAIYQISRPRIINI
                     PTNGILTKAIVDKTAAIAAACPKAQIIINVSIDGIDTQHDEIRHVPGNYKKAVATFQK
                     LKALQLPNLSVGIHTVISQFNAESFASVATELMRFEPDSYITEIAEERVELDTIGANI
                     TPSLVAYKSAVDFLIHRIKNGRFKGMNKITMAFRIEYYNLVKRIMRDHKQILPCYSGV
                     ASVQISPDGDLWSCCIKAKSLGNLRERNYNFRAIWSSPAAEIERRSIHHKECWCPLAN
                     AAYTNMLMDLPTLIRVFYRSQIKWWK"
     gene            complement(19290..20258)
                     /locus_tag="PLO35_02820"
     CDS             complement(19290..20258)
                     /locus_tag="PLO35_02820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH78627.1"
                     /translation="MKPDKRQTGTSAAPMLKCSVGIFAHNEAANIAALLRALSAQVLK
                     RAEITEIIVVSSASTDGTDDIVREYIKQDPRVRLITQERREGKSAAINLFLASATEQL
                     LVVISGDVIPAPHTIELLISALRDPSIGATGGRPVPVNDANGFMGYAVHLLWRLHHRM
                     AMLSPKLGEMIAFRKVMDAIPADSAVDEASIEALVRDAGLKLRYIPEAIISNKGPLNL
                     KDFIKQRRRIQNGHLWLMEKQGYEVVSQDKGTLLWILLDEALEYPFRVPKMLAVMALE
                     IWCRMLGSWDYRVKGRNPFAWEIARSTKDLGGVDAPLPGNDDEDKT"
     gene            complement(20288..20755)
                     /gene="nusB"
                     /locus_tag="PLO35_02825"
     CDS             complement(20288..20755)
                     /gene="nusB"
                     /locus_tag="PLO35_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOH78628.1"
                     /translation="MGQRRKAREIAVQTLYALDFAEVSQEFAEYSLLNQYPEVLQQLT
                     NSEGIEASSPLYAFADELVKNAIINLEEIEQEIDKHTENWSFESLAHLDRSIMHIAVY
                     ELMFTDTPAPVVINEAIEISKKFCSENTGKFLNGILDAINKEIKDDFHPERPS"
     gene            complement(20739..21218)
                     /gene="ribE"
                     /locus_tag="PLO35_02830"
     CDS             complement(20739..21218)
                     /gene="ribE"
                     /locus_tag="PLO35_02830"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOH78629.1"
                     /translation="MKTIEGNLVSKGYRFRIVVSRFNEFISSKLLAGAIDCLTRHETR
                     DEDIQVIWVPGAFEIPLIAQIAAEDANYDAVICLGAVIRGSTPHFEYVSAEVSKGIAQ
                     VGLEARKPVIYGILTTDSVEQAIERAGTKAGNKGFAAAASALEMVNLLREMENGTEA"
BASE COUNT         4908 a         5731 c         5675 g         4979 t
ORIGIN      
        1 agtttgggct cgaaccaggt ggatttgggg ggcatgatct caccggcgtc ggcaatcccg
       61 atcagctcgt ccatggaggt gggatacatg gcaaaggcca cggcatcacg accgctgtcc
      121 acccggcgtt ccaactcttc caaaccacga atgccgccta tgaagtcaat gcgtttatcg
      181 cgacgcggat cgccgatgcc caaaataggg gccagcagat tgctctgcag gatcgagacg
      241 tccaggctgt ccaccacgtt gcttgcgttc caggtgcctt tgcggggcac gatgcggaac
      301 caggtgtggc tgagatacat tccgatctca tgcgtcttct caggatggta aggaccattg
      361 acatacatgg cggtgacgtc gaaatccaca ctcacgcggt ccaggaagtc ctctttggaa
      421 aggccatgca ggtctttgac cacgcggttg taatcaaaga tcttgagttg gttgtccggg
      481 aaaatcaccg tcatgaagtg gttgaattcc tcgtgcccgg aataatccgg atgctgctct
      541 ctgcgcagga ggccaacttt ggcggcgctg gcggtgcggt gatgtccatc cgccacatag
      601 agatagcgca gtttggcaaa ggcattttga atagccgcaa tatcagcggg atcatccatc
      661 agccagaggc tgtggccgat gccgtcatca ctcacaaagt cataaacggg agcatgattg
      721 tccatcactt tgttgacgat ggcatcgatg ccggcctggt ggcgataggt gaaaaacact
      781 ggtgaggcgt gcgcgtcaca gacatccacg tgtttgatgc ggtcggcttc cttatcggca
      841 cgggtgagct catgcttttt gatggtgccg tccatgtatt catccacaga ggccagaccc
      901 accaatccca cctggggacg gccatccatc acctggcggt aaatgtaaaa catgggttta
      961 tcgtcttgct tcatcatgcc gcgcttgacg taggactcga ggttttctct ggccttggca
     1021 tagaccttgg gatcataaag atcggtgccc aaaggcaaat ccacctctgg cttttccacg
     1081 tggatatagc tgagcgggtt tttggccact tcggcacggg cttcatctga atccatcacg
     1141 tcgtagggaa gtgacgcgat cgctcccgca tactctggaa gcggacgtac ggctttaaaa
     1201 ggcttgaaaa ctgccatgga tcttgatact cctgtttata tgtaatattg tttcagtact
     1261 ttgatgagag acttgtggtc tttgacgcca caggtttcac gggctgaatg catggcccac
     1321 atggggttgc cgatatcgac agccgggatg cccagcaaag ctgagaccac cggcccgacc
     1381 gtgctgccgc aaggcatatc actgcgggtc ataaacttct gggctttgat gccagccgct
     1441 tcacagaggt ctagaaatct tagagtgctc tcagaggtgg aggtatagcg atagttgccg
     1501 ttgatcttga tcaccgggcc ctgattcatg gcaggagcgt agtcacggtc atacttttcg
     1561 gcataggagg gatggaaagc gtgggccata tcggccgaga tcaaaaatga gctgtgcaga
     1621 gcgcgtaact gctcttcgcg cttcaatcca agggccagag caatccgctc cagcacttcc
     1681 cccagaagtg aagaatcggc accctgctga gttccgctgc cgatctcttc atgatcgaaa
     1741 aaggcggcca gacaggtggc ctgaggggtt tgagcttctg tgagagcgct gaggatggca
     1801 tgcgtcatgg caaggttgtc cagacgtccg ctcacgatca gggacgcgtc cattccggta
     1861 agagaagatt tggcaagatc atagagatac agctccatct cgcggatgct gtccaggctg
     1921 aggtccaaat gggccgcgag ggctgaaagg agaggatttc ccgcctcgct gccagtggcc
     1981 agaatagcct gcagatgaac ctgtttgttg tattcaaagc ctttgttgat atcgcggttg
     2041 agatggatgg cggcattggg aatgatggcc aggggacggt tcatattgac gggaatagag
     2101 cgataagtat cgccggtttt gacggttacc cgtcccgcga tgcccagctc gcgatcgatc
     2161 caggtgctga taatgggtcc gccatagact tccacgccta ctctggtgac gcaggattcc
     2221 gtcttgacgg tggctggttt tagctttaaa gagggactgt cgatatggga ggctgcaatc
     2281 tgatatccgc tctccaccaa gggtttggag cctataatga aggccagaat ggcagtatcg
     2341 gccctcttca tgtaatactt ggcacccttc tccagcttcc agttctgagt ctcatcaaga
     2401 gctctgaacc cggccatttg cagcctggtt tcaatgccaa gggatgcctg aaaggcagtg
     2461 ggcgaatcat ccaaaaagga caaaagatct tgtatatggt tgttcataaa catactccgg
     2521 tatctgggtt tagggaaacc actagtttat aagggctttt tgtgtcaaag ggatttatgg
     2581 gttacgagtt aagcgctatg cgacacccgt agcgtcgaat tccaattcag cgccaaggct
     2641 ttgcatcctg cccatcacgc gtaacgcttt catcgataca gatatcgttt gatcttctgg
     2701 gtcggcgtct tctggaaggg ctcgttgacg atctggacct tggaaatgcg gctgaaatca
     2761 gccagcacct tgttcacttc ctggcggttt tcttccataa tctggggtac ccgggcctct
     2821 gggatatgat ccttgtccag ggattccagg tcaggataga tgagagcatg cagcttacga
     2881 tcgcgttcca gcaccagggt ttccatcaca taggggaggt tgttgagctt ctgctccacc
     2941 agttccggat agatgttttc accgctgggg ccgaggacca cgtttttact gcggccgcga
     3001 agatagataa agctgtcttt gtccagagtg ccgatgtcac cagtgtagag ccatccgtct
     3061 ttcagagttt cactggtggc ttcctcattt ttataataac cggaaaacac ctgttccccg
     3121 cttatcagca cttcgccagg tattttggcg ggatcggaac tctcgatctt gcacttgatg
     3181 tatttgacca gcttgccact ggattcaaag cgatgctcaa accagcgggc ataggagatc
     3241 agggggccgc actccgtcat tccatagccg attgtgatgg ggaactttat gcgtttcata
     3301 aagagctcca cttctgcatt catgggtgct ccgcctacca ccagttcggt aagctcacct
     3361 ccgaaggcag tgaccagctt gtctctgatc tttttgcaga ccagtttgtt gagcagcggc
     3421 aacttgagca ttgccttcat tttgggggtt tcgatgacgg gctgcagctg acgtttgaag
     3481 atcttttcga tcagcagcgg tacggccagc accacattgg gtttgaggtc ctgcatagcc
     3541 cccagcagca ccttgggagc agggatttta tcaataaagt tgatatgatt gccgcgggtg
     3601 aaaggataga ggaggtcaaa gctgcaggca taagcgtggg ccaaaggcag aaaggccacc
     3661 actttagctc cttccttgaa gagcaggttt tcccgggcta caatgatatt ggccagcaga
     3721 ctgcggtgag acagcatcac gcctttggaa aacccggtcg tgccggaggt gtagataatc
     3781 gcaccgatat cttcgttgtc gcacccgtct tccacattca tgccgccagc gctgagtccc
     3841 ttgagatcgg tggtctcgct gatgggtttg atgatctttt tgatcgtttc atccctggca
     3901 aagaggaggc tgaaatcatc cagtgaaaag atgtatttca ccttgcgcag ctggtccgga
     3961 tcgatgctgt catacttgtc tttggagacg aataatatct ctgcctcgca gtggtttacg
     4021 atgtgctgtg tatcctccgg agagaaattg gccaaaatgg gaacgatgct gcccccatag
     4081 gtgacggtag aaatccacag ggtagcccag tttccactgt ttttaccagc cagggccact
     4141 ttaccgcttt ttttcatgcc gcaggcttta aagaggcggt gtatccatag cagttgtttg
     4201 ccaacgtccg agtatttcaa agcgggacca gggtagtccg taaaggctgg catgtcccaa
     4261 tagttcttga tcgaatcgat aagataaggg atcaattttt cctggatcat ctatctctcc
     4321 taaggtcaga tttggataat tcacctgacc cgaaagctgt gaatctgtca agcgggattt
     4381 aaggatttag ggttttaagg ttttagggag atgcttcgca tgtgagagtt tgacggctgg
     4441 ttagagcggc gctaagacga gcgccgatat atctcaacct tctcaacttt cacagctctt
     4501 taaaccataa aaccctaaaa ccttacaacc ctagaacccc acaagcacca tttccatctg
     4561 ccttgcaggc ttgcccgata cccgccgtgg agatgccagc ccgcctccgc cgaaaatcgc
     4621 cggagtgcgc acggcatctc cacggcggat gtataggcaa gaacagagtg aaaccaaaaa
     4681 tactattaca aagccccgcc attatgacgg ggctgagcta tgaatggatt caggcgatct
     4741 attctttggg catttcaaag cggagcgcga cgtattggaa catattgttg cggtccttca
     4801 cgtagagcct gatggtggat tttccactct tggacatggc gtcgatctga ccgtccatgg
     4861 tgagcctgaa ctcgtcggga ttgttaaccg ggaggttgtt gaccctgaga atcaccaaac
     4921 ccacggaaag gcctgctttg gcggctgggg atcccgcttc cacggctgtg atcaccacgc
     4981 cttcggtgcc tgaaatgttg agacgttggg ccgcggtgct gcccatggcg tccaccgtga
     5041 tgccgtgact gagtttgacc tcaggggcaa tctctccgtt ggcagctttg tcctcaggga
     5101 agtaatccag ctcgatattg aggctgagct cctggttgtc gcgaattatc aggacgggta
     5161 cgatgcttcc ggccttggag gtggctaccg cgatgcgaaa tttggccacg ttgggcactt
     5221 tctcacggtt gaattccaca atcacgtccc cggtctggat gccggctttg gaggcgggag
     5281 aatcgtcttc cactttggct accaggactc cgctaacctc cttgaggtca aatgcttcca
     5341 tcagatcgga ggtgatctcc tgaggcgaga tgccgatgta ggcccggctg acttttccgg
     5401 tagcaaccag atcgtccacc acacgtttgg cgaggttgat gggaatggca aaaccgatac
     5461 cgatattgcc accgctggtg ctggtgatgg cagcattgat gccgatcact tcaccgtaga
     5521 tgttcagaag agggcctccg ctgttgccgg gattgatggc agcgtctgtc tgaatgtaat
     5581 cctgatagat gggggaacca gcgccaaaat tgaggttgga acgtccgatg gcggagatca
     5641 ctcccagggt tacggtgcgg tccagacccg cttccccgaa gggattgccg atcgcgatgg
     5701 cccactcccc gatttcgagg ttactggaac tgcccagggg ggccacggtg atcttttcgc
     5761 cttctttgac ggtgattttg atcacggcca cgtccgtatt gggatccagg cccaccacct
     5821 ccgcgttgta attgaccttg tctgccagag taacggtgat ggttccggat ttaccctgct
     5881 ccaccacgtg gttgttggtc atgatgtatg cttcccgcgt ttggggattg tagtcataga
     5941 caaagccgct gcccatcgaa gtgacaggcc tctgctgagc gcggggctgc tctggaaaga
     6001 aatagcggaa gaagggatcg tcgaagaact ggttcatggg gttttggacg gtgatcttgg
     6061 cttcaaccct gatctgaacg acggcttcac gaacgcggcg gacaacctcc acgacgggat
     6121 tctgggccgg ttggatcatc tccggggccg cggaacgggc ctgcagaact ggcgcttgaa
     6181 atgaggtggg ctcctcctga gcgttggcgc aggaaaacag caccaggatc agggccagcg
     6241 gaacgatacg tttccacaat gtcataagat actcctgtat tatatatgga ttatggtagt
     6301 tgaaaactga gtcatgtcta aatatacaaa ctacaatagg ctccgtgctc acgggctttg
     6361 atagggacaa tttaatgctg cttccggccg cggtcaagaa aaaaatgctt tacaggtgag
     6421 agctgtgctg tgagagcttc ggctggtgag atactgctta gcggacggca ccctaaaact
     6481 gtaaaaccct aaaaccctaa aaccttaaaa ccccaatacc ctactctgtt cctctctctg
     6541 tgccctctgc accctctgtg gtaaaaaaga aaagctctgt atctctgtgg tcaggacaca
     6601 cgctgaattg gaatttgacc cagcgacctt gtgccgcttg acagatttcc cgctctttgt
     6661 aaaggtggct gacatggaaa aaaccaaacc ggagttcact cgcgacacca acacggaaat
     6721 caaattcctc aagggagttg gggacgcgag gggtcgtatt ctggccaaac tgggcatcag
     6781 acgggtcctg gacctgatgg aacattttcc ccgatactat atcagccgta gcatcgatcc
     6841 cacccttcac gaagccaaag tgggagacca cgtggccttt accgccaatg tttcctgggt
     6901 ggatacccgc accaccaaaa acggcaaaaa gatcctcaac gtgggcatca acgacgtcat
     6961 gaacacgctc acctgcagct ggttcagtta ccccaactgg tatgaaggca tcctggtgcc
     7021 gggcaaacga atctgggtga atggagtgat cagtgagttc aacggccagc ttcagatgct
     7081 gcatcccgag atcgaagcgg tggatgacga cacggaagac gacttttgga agactcgtct
     7141 ctgcctgcca gtgtatcccg ccacggcaaa tctgaaccag aagaatctgc gccggctggt
     7201 ctggcaggcc tttcacgatt atgcctccac tattgtggag aatctaccca cctatttgat
     7261 cgataaatac cagtttaagg accgcaagac ggcactgcag ctgatgcatt tcagtcagaa
     7321 tcccgatctg ccagtccagg tgatgacgcg ctttgcctat gaggaatttt tctttgccca
     7381 gctgctgtgg gccaggcatc gaaagtatca tacagacgag gtgaagggaa tcgcgtttga
     7441 aaaccaacgc aggctgactt ccgcgcttaa aaacaagctg ccctttgagc tcacgaaagc
     7501 acaaaaacgg gtgatcagag agatctttga cgacatgtgc tcccctcgcc agatgagcag
     7561 actgatgcag ggtgacgtgg gttccggcaa gacggtcgtt acactctttg ccatgctcct
     7621 ggcggtggaa aacggatatc aggctgccct catggccccc acggaaatcc tcgcggagca
     7681 gcattttggc acgatcacaa gcctcctgga aggcatggat gtcaggatca ccctgctaaa
     7741 aggcggttcc tacaaaggta aggcgcaaac cgtaactgac atagcggaag gagcttatga
     7801 cctcatcatc ggcacccatg ccttgattca agataaggta acatataaaa acctgggctt
     7861 tgtggcggtg gacgagcagc accgtttcgg agtgcagcaa cgagccgctc tggccagaaa
     7921 agacaagcat ccggacctcc tctacctttc cgccactccc attcccagat ccttgtccat
     7981 gacagtttac ggcgatctgg aagtgagcgt catcgacgaa ctgcccccca cccgaaaacc
     8041 ggtgctcact ctgatccgtt cggaccgcaa aattgaactg gtgtgggcgg acgtcaaaaa
     8101 agagcttgca aaaggccgtc aggtctatgt ggtctgccct ctggtggagg aatccgacaa
     8161 ggtggatttg ctggacgccc agaggctgca tcagcacctg agctccaaga ttttcaaggg
     8221 catcagttgc gagcttttac atggacgcat gccagcccgg cagaaagatg agatcatgaa
     8281 gcgctttaaa gccgctgaga taatgatttt ggtgtccacc accgtgatcg aggtgggggt
     8341 ggacgttccc aacgccaccg tgatggtagt ggagcacgcg gaacgctttg gcctggcaca
     8401 gctgcatcag ttgagaggca gagtgggacg cggagccgaa caatcctgct gctatctgat
     8461 cgagcatcag ccctgttcaa agatcgcctg ggaacgcctg gacaccatgg ccaggactac
     8521 cgacggcttc ctgatcgccg agaaggactt ggaactgcgg ggcccgggtg agttctttgg
     8581 cacggaacag gctgggctgc caaacttcaa gtttgccaat ctgatccgcg atcagcatat
     8641 cctcaagctg gccagaaacg atgcctttga gatcatcacc gcggacccgg acctgcaggc
     8701 tgaacagcac caggtgctga aaaaaatcct gactacagag ttggctgata aagagaagct
     8761 tatcctctat tagtgtctta tgcgaaagta aaaagggttg acgtatttgc cgcatacaga
     8821 tatcgtgcct gaaaagtaaa ttcccgaggt gcttatgaag aagctgaccc ttatgatctg
     8881 cctgatcatt gctataagcg cggttttcgc ggttaagttt gatccttatt acttccagtc
     8941 caagacgatc atcacctgct tcagcagagc ggcgatcggc aacgacgccg gaacgataga
     9001 attcacgaaa caagatggtg tagtccgtac cggcatcagc agtttcgatg cgctggccag
     9061 ccagtataag atagtggata tgaagcagat gcatccctac gtcaaagtcc ccgagtggaa
     9121 tgacaagggt gcctatctgc aaaaccacta ccgcgtctat ctggaatccg acgacaacat
     9181 cgatgccgcc gtcgcggccc tggccaaaga ccccaacgtc atatacgcgg agctggaagg
     9241 catcaaccgc agcaagtttg tgcccaacga ccccatgctc acctcacaat atgtgcatgc
     9301 ccgcatccgc agctacgacg cctgggatta tattcagggt tcttatgacg tcaaggtggc
     9361 aatcaccgac tccggtgtga aatggaacca tccagacctg agggccaata tttgggtcaa
     9421 ccctgccgag tctcctggaa tgacgatcaa ctgggacgcc ggaacgatca caggcggcaa
     9481 tggatctgac gccggtgaag gtggcagcaa agttgacgac ctggttggct gggactttta
     9541 caacaacgac aacaacccca tccagaccta tgccgccaat gaccacggaa cccacgtggc
     9601 tggctgcgcc gcggcggttg gaaacaacgc catcggtgtg gtgggcactt ccccaatcgc
     9661 ttccatcata agctgtaaag gagcctccaa cacatcgccc agcactggta tctcctatgc
     9721 ttacgaccag gtgaagtatg ccggagagat tggcgccgac atcatcaacg ccagttgggg
     9781 tgggccaggg accggcgcat atccaaacag catcgtcaat tacgtcactg ctttgggtgc
     9841 tttggtggtc acagccgctg gcaacgaaaa cactgagcac accgcctcat atcaggatta
     9901 tcctgccgac tgcacaaacg tgctgtgcgt ggcctccacc ggtcagaacg acgtcaaatc
     9961 aagtttttca gactacggga caaccgtaga tatctccgcc ccaggagaag gaatcctctc
    10021 cacaattgtc gccagcaatg gatatgactc ctatgacgga acttccatgg cttctcccaa
    10081 tgtggctggc gtggcggccc tggtgaaagc cctgcacccc actctcactc ccgcgcagct
    10141 gatgcagcgt ctgatgatga ccgcggatta tatctatgaa gccaatccca gctatgtgga
    10201 caaactgggg gcgggcagag taaatgcttt caccgcaacc atgtttgaca agatccctta
    10261 catcaccata gacgatacca tggtggagga acaaaccggc gacggggacg ggattcccaa
    10321 tgccggagaa accatccgcc tcaaggtatc catcaacaat ctgcttgatc cctttaccgg
    10381 gctggcctgg cagcctgcca ccaatctcca gaccactctc accaccacct atcccggcat
    10441 caccattgtg aacgacagcg ccaattatgg caccctctca gccggaagca ctatgtggaa
    10501 caactctcag cctttcatct tccagacggt tgccaacctg ccctccgagc cgattccttt
    10561 tgaactggta gcctctgcca atcagggttc cgccttccca tacaatgcca ccatcccatt
    10621 caccgtgagg ctttccaaca atcaggccgg ctggcctgtc ctgatcggtg gcgccaccaa
    10681 ttccagcccc attatcgtga atctggatgc cgatcctcag agagaaatgg tctttagcga
    10741 ccaccttggc aatatccacg ccctcaatcc caacggcacc cagatgccag gatttccgat
    10801 ggccctcggc tccagtgtga tgggatcgat cgccatgagc gatatcgaca gcaacggtca
    10861 gaaagaattc gccgccagcc tgcaaaacaa caacctcgcc ctgttcaatc aaagcggcca
    10921 ggtgatcttc aacatccctg ccggcggaac tttgcgcagc ggtcccgtga tcacttccct
    10981 ggcccaaaac tccagccgtc agattgtggc cgccatccaa agcggcaaca tcgtggtcct
    11041 cgacaccaat ggcaatgcct atcccaattt cccggccgga gtgggtggag cgattttggg
    11101 ctcgcctgcc atcgccgatc tcaacaacga cggctatcag gagattttgg tagccaccct
    11161 caacggcaac ctgcatgcca tcaacagcca gaccggccaa aacattgccg gcttccccat
    11221 caccatgcag ggcggctctc aaaaccccat caccattgcc aatctcgatg ctgacaacta
    11281 tccggagatc atcgtctcca ccagcacggc tggctatatt cacgcctaca accatgacgg
    11341 cagcgtgttc ttccagaaga atgtgggcgg ttccatcaaa accggggccg tggtggcgga
    11401 tgtcaactcc gacggcaaca aagagatcgt cgtgatcagc aatgccggaa tggtctatat
    11461 cctcaacgcc aacggcaccg atctggccgg cactcccatc aacgtcaatc agactgtgga
    11521 ctgcacccca gtagtggcca aatttgacgg cgacaactat gccggcatca tctttggaga
    11581 caccaacggc aagcttcaca gcgtgagggc caatggcacc cagtctccca atttcccgat
    11641 cacaattggc ggaaacatca aaatctcggc cgccctctcg gatatcgatc tcgacaacga
    11701 catggacatc gtgatcccca gcgataccgg tttctacgtc ctggatatca agcgccccgc
    11761 ccaaagctat gaatggccat gctttatggg cagctacaac cggggaggca acgtctatca
    11821 ggccactcct tcagacgatc ctgtggtacc agcctttgcc gatgccctga ttggcaacta
    11881 tcccaacccc ttcaatccca gcaccaccat cagcttcagc cttgcgcaga gcggtcccgt
    11941 gagcctggag atcttcaacc aaaaaggcca gaaagttcgc gatctcagca gcggaaatct
    12001 gcctgccgga gagcatcagt tggtgtggaa cggcaaagac gacagtggca gaccggtcag
    12061 cagcggcgtt tactacttcc ggatgaagag cggtaagttc tcttcctccc gcaagatggt
    12121 catgatgaaa taatcaggcc caggccttca taagcatcca gcccggcact cgctgccggg
    12181 ttttttttgc aggcccgtcc cgaaccgctg taaagatcag aaatagcttg ccagagcctg
    12241 cagaatcgct attatgtcac atattgattg acggtggtct caagctccac cgcacccaag
    12301 gagatacctt tcatgaagaa gatcatgatc ctgctcattt tgatcgtggc cagcacggcc
    12361 gccttcgctc agaccggact ttttgacctc agcttcgggg acagctttga aaaatgcgaa
    12421 tcgctcctca aatccaaagg cttcagcaac aatatcggcg actttggccc caactatttt
    12481 gagcctgacg atctgggcta tgtggcctat atagagctaa acctggatga taccaggcat
    12541 ttgaaaggat ggacggtagt gtataccggc gaggttcacg acgactatga agaagtggcc
    12601 atggacgcga taatctcctg gcacggggaa gactatgacg tggattggga tgactgggat
    12661 tttgagtact atgaatggga gctcgaaaac ggcaggtatg tggaagccgt ttgggatgag
    12721 tacttctacg tgtactacta ctgattccag gccctcgctt gtcacctgga cctaagctca
    12781 tctatagcaa gccctaccca tccctccttt gttggggatg ggtcagggat gccagaccgg
    12841 aacccggggc cagtgaacag ggcccagccc agaaaagcta taagcagata tagattgaca
    12901 gaattctccc atctgacaca ggtagctcag gagctaagat gtataaaccc atagtattgt
    12961 tgatctgtgt ttcatgctgc gttgggatac tcaacgcgca gggtggtctt tatggtcttg
    13021 actggggggc cagtcacgaa gccgcgtccc tgatcctgat cgatcagggt ttcaaaatcc
    13081 aagcccacac cccggagcaa atcatcatgg tcccgcttga cgaagagccg atcaaaggaa
    13141 tagaattgag atttgacggc caaaaggaca gattggcggg ctggagcata ggctattcct
    13201 ttctggaaga agaggatatg ggggagctgg tgattggcgc gctgatcagc cggcatggcc
    13261 ctgactttga aaaaacaact gaaggcatct acctttggca gctcggaaat ggccagacag
    13321 tgagggccgg ctgggactat ttcaaagcta tgtttgtggt tgattaccgc caggagagcc
    13381 tggaagctcg ctgacggctc agcccaggat aacgtccagc accatcatca cgcaaaagcc
    13441 cagcatcaat cccgctgtgg ccaggtgccc atgtttgccg ccctgggctt ctggaatcaa
    13501 ttcctccacc accacataga tcatcgctcc ggccgcgaag gccagtgtgt atggcaggat
    13561 gggcttagcg ctcatggcca gagcggcacc caacacccca cccaggggtt ccacaatccc
    13621 ggaaaattga ccccaaaaaa agctcctcca gcggctcatc ccctctctgc gcaaggggat
    13681 ggacagtgcc gccccttccg gaagattctg aatcccgatt cccaatgcca gggccagggc
    13741 cgcggaaaga gtcgcagcct caccgccata agccagggct ccaaaagcca cccctaccgc
    13801 cagtccttcc ggaaaattgt gcagagtgac agccaggaac atcagagtgg atttgcccca
    13861 gggggttttt agcccttcct tttcgatctg cccgtggctt tcatgcacgt gcggaagcaa
    13921 gagatcgatc accctcagaa aggccccacc caccaaaaat ccgatcacgg cgggccagac
    13981 actgccattg ctcatatcga tggctggatt gatcaggccc cacacgctgg ctgctatcat
    14041 cactcccgcc gcgaaaccca gcatcgtatc caggacggat tgccttatgc tcttgaaaaa
    14101 aagcacaaaa ctggccccca gggctgtcat gccccaggtg aacagggtgc ccagcagggc
    14161 caggaggatt ggtttatcca caggtatgtg catagaggtc tcccttgctc gcgcttaagt
    14221 cctaacggtt atagaagctc tcaatcgctt ccaggatctg agtcatggcg gctttggtgt
    14281 ctgggacgtc gatcgcgcgc ttatcaccat tgtcgaatgt aactaccaga ttccccaggg
    14341 cctgagaaat ctctctgatc ttggggatca cgatatacat cttgcccaga tgctcgtccg
    14401 tgtaggtaaa gatagatttc ataagctcct caatagcgtt tgaagctgca gctctggctg
    14461 tggttctcgc cggggcgcag atattccaca tattccggat caaactcgat gagagtgtag
    14521 tccggatcgt catatccgcc aaagaattga tcgaagaaat aacagtgttc agccgcctcg
    14581 gctttgagag aagcgttttg gaccaccgaa gccgtaccgc tgaggcggag gtagccgttc
    14641 tggccatcct ccgcgatcgg tacgcatact tcaacccatt tattggcatt gatttggccc
    14701 actttggaat caccgctgaa ggtggtaaag aaatagcggt cgtcatagcc atacatcacg
    14761 atgggacgca cgcgcggctg tttcttatcg accgtggcca gatacacata ctggttttgg
    14821 cgtatccagg ccagaatctc catcatgaga gcggattccg aagcgcgggc catgactcaa
    14881 actccttact tgggcaggac gatactgttg ataaaggcct ggttttcgag gttgggattg
    14941 tcggcccggt cacgaggcag gttttcgatc ttgtggacta catccatgcc ctgggtgact
    15001 cttccaaaca cggtgtgttt gccgtccagg tgaggggtcc cgggtttgcg ggtcacgata
    15061 aagaactggg aaccattggt gttggggccg gaattcgcca tcgcgatgta ggaatagagc
    15121 acaggggacg cgaggatctg accatagagg ggatcattga tgccggtgcg gctttgaaac
    15181 cattccaccg tcttgccaat tatagcggcc ccgctttgag tgttctgaac ctgttttacc
    15241 acctcaaaga tctcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngatagt
    15361 catgaccgcg cgaagcggta aactagcttg tctcattctg ctttctcctt atgtttgtaa
    15421 tgatttaagt atatcttgcc atccaggatc tccagatagc tgtggtttct tagccagtca
    15481 ccggcattgg cgtaaacgcc agagcccacg ctgatgatct cgggctcgtg gttgtggccc
    15541 atgatcacga tatcggcttt gccttttgag atctgagctc tggcccaggt cttcaagcca
    15601 ttgcccttgc ggcgtttgag aacgtcagac atcctgcgca gacggctgga gcgggacatc
    15661 gcggccccga tcgtgagggc caggtcagga tgcaagagat caaagctgag gcggaccagc
    15721 ggggtcctga tcaaacgcct gaacagcttg tagcgcaagt cgttgacggt atagaggtca
    15781 ccatgggtaa agagcagctt ttggccgtcg gccctcaggg aatagtggtc gtcgtacagc
    15841 tcgaatccca gctctttatc cagaaagccg ttgaaccaga aatcgtggtt gccgctgata
    15901 tagactattc tacaacccgc ttcaaggata ttggccagcc tgtgcagcac cggaaaatac
    15961 tgacgcacga tcgtgtattt ccagtcgttg tagagatcaa agatgtcacc attcagcacc
    16021 agcagatcat agcgtcccgc gatcccctcc agaaagctca gaaagagctg tgagttttct
    16081 ctgtcggcag ggctttcctg cctgtattta taatgtacgt ctgatatcac acagatgttc
    16141 atgtcagcgc gtgatgagat gatagacgat attgacccca aagcgcagtg cggtctcacg
    16201 cgtttcggga gggttgttgt gagtgccggg atcggcccag ccgtcgctga tattactttc
    16261 gtaggtgtag aggcacatca gcctgccggt gtcgtcaaac actccaaaag cctggggcgg
    16321 cttggcgtcg tgctcatgga ttttgggcag tccggaactg aaatcgaaga atgatctgaa
    16381 cagcggatag gcaggattga gctccacgag gctgtgctcc ggaaagatcc ttttgatctc
    16441 gcgccggaag gattggtcca tgccataatc gtcgtcggca tacagaaatc cccctctttg
    16501 catccattgc ctgaggtttt ccacctcgcg atcagtgaaa cggatgtttc catgcccggt
    16561 gagatagaca aagggacaat cgaagagccg cgcatccgct gctttcacca ccgtctgatc
    16621 gataggaaaa gtggtgccca gagtggcatt gacgaatctg gccagattgg gcagcacctc
    16681 gggatcgttg taccaatctc ccccaccctc atattgaaga cgggcaaaac ccgctctggt
    16741 gatgcttcca gtcaccgcgg ccagacttgc cgccgccagc attatggtca ggatgaggat
    16801 cagctcaata tctttaactc gagacatatc atggtatcct gcaccaccgc gtaaaagcag
    16861 ttctgaaagc tgtcgtaggt ggagagatat tgcagctcgt ccttaaaagt gatttcactg
    16921 agcttttcac cgctgttgcc gtccagcacc agaatagaac gctgggtggg caccacgatc
    16981 aagcttccat cgatgcgggg atcgtggata ttggtgagtt ccataggttt gagcgagtgg
    17041 aagatattca cctgagagcc caggggctgt tcccaggata gctctccgtt gttgttgagg
    17101 cccaggacgc gctgatcttc cagattgatc atcaggccgc ctgtgatgcc tgtgatgccc
    17161 tgcaccttgc ttcccaggct tttattccac agcagattgc gccctttttt gttgaaagcc
    17221 agcagcatgt cgttgctggc cagatagata ttgtccagat caaatcccgg ggcagcggtg
    17281 acctgatagg gcagatcggt gttccagatc agcttgagat ccacttcccg ggcgggactc
    17341 ttcagagcct gttgcagata gtttctcacc ttctgattgt cgatattgag atagttggga
    17401 atctgctcca gcgggatggg tttcttgctc tggaacagca tgcgcgcttt gcgccaggtc
    17461 atctcaaaaa agcgtccccc aaagatcaca tacacgatcg ccaggagggt aaaaaagatc
    17521 aggagaatag tggccagatg caagcggctt aagcccttac gcttggtctt aatgtgctcg
    17581 ggctcgttac ccagtaacaa ccatagcgca cccggtttgc cagccaggga atccagcaaa
    17641 gcgttcacgc tgccgccgtc gaaatggccg ccaaacagct ctctggccat agcacctggc
    17701 agcaccagca ggaaatgccg ctccggcacc aggactttct gggtctttac cctgatttct
    17761 tcttccgaga ggcccagcag gttgagctgt tccagggtct gcaaatgtga atgttcccag
    17821 tctttaccga tcgcttccgt ctttttggga cctcccagcg tgcaaaacat cctgccaaac
    17881 tgagtgaaga taatctcgtt gtcatacatc aatcccaaaa acagcgaaat ccctttctcc
    17941 ttgagctcgg tgcggcgcag cctggcatgc agtttccagt ggaaatcggc aaagaagctc
    18001 ttgagccact tttccgcctc cagtttgctc acctggcgga tatccgaatt catcagctcc
    18061 agcttgattt cagcctggat ttcctccaga atgagatggc tttcggggtg ccaggtgcag
    18121 aggaaccagc catagcggcc gtctttgtcc acctgatatt cacaggccgc ctgaggcggg
    18181 ttttgagctt tgctgcgcag ggtaatcata tcatttccac catttgatct gggaacggta
    18241 aaagacgcga atgagggtgg gcaagtccat caacatattg gtataagccg cgttagccag
    18301 aggacaccag cattctttgt ggtggatgct gcggcgttca atttcggcgg cgggggagct
    18361 ccagatggca cggaaattgt aattccgctc tctgaggttg ccaaggcttt tggccttgat
    18421 gcagcaggac cacagatccc cgtccggaga tatctgcaca cttgccactc cgctataaca
    18481 aggcaggatt tgcttgtggt cgcgcataat ccttttcacc aggttataat actcgatcct
    18541 gaaagccatg gtgatcttgt tcatgccttt gaaacggccg tttttgatgc ggtggataag
    18601 gaagtccacg gcgctcttgt aggctaccag agagggagtt atattggctc caatggtatc
    18661 cagttccacc ctttcttccg cgatctcggt gatatagctg tccggctcga agcgcatgag
    18721 ctccgtggct acggaggcaa agctttcagc gttgaactga gaaatcaccg tgtggatgcc
    18781 cacgctgagg ttgggaagct ggagggcttt gagtttctgg aaagtagcca ccgctttttt
    18841 gtaattgccc ggcacgtgac ggatctcgtc gtgctgggta tcgatgccat cgatcgaaac
    18901 gttgatgatg atctgagctt tgggacaggc cgccgcgatg gcagcagtct tatccacaat
    18961 cgccttggtg aggatgccgt tggtggggat attgatgatg cggggcctgg aaatctgata
    19021 gatggcactc accacttcgt cgatatcgtt cctcagaaat ggctctcctc cgctgaaggt
    19081 gacccaataa ggcgttctcc cgatcccttt gaagattttc ctgtattcat ccaccgtgag
    19141 gttttgggcc ttctttttcc agatgttgca ggtgccacag cgggaattgc atgtatataa
    19201 aaggcttacg gtgtagttta tgggcagcag tttgggaaag cccagatgct tcataaacca
    19261 gtagcccagc atgcgaaaca tcaaccagat catgttttat cctcatcgtc attcccgggc
    19321 aggggagcgt caactcctcc caggtccttg gtgctgcggg caatttccca ggcaaaggga
    19381 ttgcgtccct tcactctgta atcccagctt cccagcattc tgcaccagat ctccagagcc
    19441 atcactgcca gcatctttgg cactcgaaaa ggatactcca gggcttcgtc caggaggatc
    19501 cagagcaggg ttcccttgtc ctgagacacc acttcatagc cctgtttttc catcagccag
    19561 agatggccgt tttggattct gcgccgctgt ttgataaagt ctttcaggtt caaggggcct
    19621 ttgttgctga tgatcgcttc gggaatatag cgcagtttga ggcccgcgtc cctcaccaag
    19681 gcttcgatgc tggcttcatc cacggccgaa tcggcaggaa tggcatccat cactttgcgg
    19741 aaggcgatca tttcacccag cttggggctc agcatcgcca tgcggtgatg caaacgccat
    19801 aaaagatgca cggcatagcc catgaaaccg tttgcatcat tgacgggaac cggccttcca
    19861 cctgtcgcgc cgatacttgg gtccctgagg gcggaaatca ataattctat cgtatgaggg
    19921 gcgggaatca cgtctccact gatcaccacc aggagctgct ctgtggcgct ggccagaaac
    19981 agattgatgg cggcggattt gccttcgcgc cgttcctgag tgatgagccg cactctgggg
    20041 tcctgcttaa tatattctcg aacaatgtca tcggtgccat cggtgctggc gctggagacc
    20101 acgatgatct ccgtgatctc agcccgtttg aggacctgag cgctcagagc tctcagcagg
    20161 gccgcaatgt tggccgcttc gttgtgggca aagatgccca cgctgcactt caacattgga
    20221 gcggcagacg tccccgtctg ccttttatct ggcttcatgg gctcactaac ctttgtatcc
    20281 gcttccctca tgagggccgt tcggggtgaa aatcgtcctt gatttccttg ttgatggcgt
    20341 ccaggatgcc gttgaggaat tttccggtgt tttcgctgca gaatttcttg gagatttcaa
    20401 tggcctcatt gatcacaacc ggggcgggag tgtctgtgaa cataagctca tacacggcga
    20461 tgtgcatgat gctgcggtcc agatgggcca ggctctcaaa gctccagttt tcagtatgtt
    20521 tgtcgatttc ctgctcgatc tcctcaaggt tgatgatggc gtttttgacc agctcatcgg
    20581 caaaggcata aaggggagag ctcgcctcta tcccttcgga atttgtaagc tgctgcagca
    20641 cttccggata ctggttcaga agtgagtatt ccgcgaattc ctggctcacc tcggcaaaat
    20701 caagcgcgta gagggtctgg accgctatct cgcgagcttt acgcctctgt cccattttcc
    20761 atctccctga gcaggtttac catttccaaa gccgaagcgg ccgcggcaaa acctttgttt
    20821 ccggctttgg tgccggccct ttcgatggcc tgttccacgc tatccgtggt caaaatgccg
    20881 tagatcaccg gttttcttgc ttccaggccc acttgcgcga tacccttgct gacctcggcg
    20941 ctcacatatt caaagtgagg ggtgctgcca cggatcacgg cccccagaca gattacggca
    21001 tcgtaatttg cgtcttctgc cgctatttgc gcgatgagcg gaatctcaaa agcgcctggt
    21061 acccagatca cctggatatc ctcgtcccgg gtttcgtgac gggtgaggca atcgatagca
    21121 ccggccagca atttactgct gataaactcg ttgaaacggc tcaccacgat cctgaagcgg
    21181 tagcctttgg aaaccaggtt tccctcaata gtcttcatta tatatgctcc ttttaaaggg
    21241 cttttttctc tgaaggtcaa gaaaatccag agcccaaatc aatcaaggaa aatctttctg
    21301 agtgccacct ggctggatta cactcatgtt tgcattgcct tcgcgtcact gccacttctc
    21361 ctatgcccag gcaaaggtga tgtaaaggag ggg
//
LOCUS       DAOSFN010000023        20045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2175_length_20045_cov_8.507304, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000023 DAOSFN010000000
VERSION     DAOSFN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 20045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20045
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2175_length_20045_cov_8.507304"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..844
                     /gene="rimP"
                     /locus_tag="PLO35_02835"
     CDS             368..844
                     /gene="rimP"
                     /locus_tag="PLO35_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOH78630.1"
                     /translation="MEYYRVQIEEIAKRICSEKHLALYDIDEKMSGKGRIITVYLTKI
                     GGVTLDECASFSRALGAELEEFDLIPERYYLEVSSPGVERPLKLKSHYVSAINEKVAV
                     QFNDGEEKKSVMGTLTVVDQDEITIDDRGTLHLIPFKQISRAKTVYIPDWSETDER"
     gene            834..2066
                     /gene="nusA"
                     /locus_tag="PLO35_02840"
     CDS             834..2066
                     /gene="nusA"
                     /locus_tag="PLO35_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOH78631.1"
                     /translation="MSANILDAVIKLAAIKQLDKDQIQRIIEEAIFSTLEKRLGPDNE
                     LTVFADQATGKIQARFLCRVVELEENLGEISLAEARTLNPSVQLEDMIQRSMSLYEFE
                     PKLVKNAQRLIMEKIKELEDEKIQKDYNKLKNTIVVGKIKTVDENGGYRIDINHTDAL
                     LPPDEQIENEYYRVGENIRAYVVNIRSHQSNITIILSRTNPEFVKKLFEAEIPEIYSG
                     QITIRKIVREPGIRTKVELETKDPKLDPISACVGIRGTRIDGIRRELHGEQIDIVVHS
                     DDPEQMIINALGIDGAINVIIERNRAASVIVNEDNKVIAIGKQGKNVKLAAKLTGMKI
                     DIFTSAEFDEKMAKERRTVSHITELDGVTAKIAEALKQAGYTSVQDVFAASLDELVNL
                     EGVGQKTAERLKEAAKYF"
     gene            2066..2320
                     /locus_tag="PLO35_02845"
     CDS             2066..2320
                     /locus_tag="PLO35_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HOH78632.1"
                     /translation="MPNHNSKAGHQAQRTCVVCRGKNALDGMLSFILLDGKLVFDLGG
                     ALQCRKFHLCPGPECVEGLPVWIKRYHKKRAGSGSRRGQK"
     gene            2317..2835
                     /locus_tag="PLO35_02850"
     CDS             2317..2835
                     /locus_tag="PLO35_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOH78633.1"
                     /translation="MTATPKKHPRPARSSSAPRGAGSQPGAKKLPAPRPRVTPAQASD
                     SLPVKARARQTIAVTAPQLKKMTTLMQFARRAGKLISGYDACLRAMHQHKLYLVLLAE
                     DTAPRTCRGIDHALNENQIAIPLMRAGNQTDLSQALGLPKSGVFGIGDKQFAKAIQAC
                     AGMEPRREEPCK"
     gene            2826..5501
                     /gene="infB"
                     /locus_tag="PLO35_02855"
     CDS             2826..5501
                     /gene="infB"
                     /locus_tag="PLO35_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOH78634.1"
                     /translation="MQIRVHELAKELKISTMALKKHLIDLGVVTKSHMSFIEEDVADK
                     IRRKYNEQVDAEKRAEKERKKFIEMRQAAKHKHEDIVIPEPVKEPEPEAAPAARPTAK
                     TKAAPAEVSEEHASSEPSEAPDPKQEAEPEVPVTREEPVTVPEAPVKPVPEAPKHRII
                     VPYTQATPVQPRGPRERVRPKSYLETQTARSGPKPPASGRPSPGTRPRPDSRKPGESL
                     PDVKPLPVTPAAAKEAEEKKFGKAKVSHEELGDKSKHKKAIITSTKKTKQKVAEPTEV
                     DEAAISRNIKKTLQKSSKRKKYHREAQAVDTSSTEIVIREYTSVSELAKIMNVSAAEI
                     ISKFFMMGQLVTMNQRLDKDSLEMICDEFKVEFRFEDEYGADIIDRERESYQDIEAET
                     RAPVVTIMGHVDHGKTSILDYIRNTNIVAGESGGITQHIGAYQIEINKHKITFLDTPG
                     HEAFTAMRARGANVTDIAVIVVAATEGMKPQTKEAIDHAKAAGVQIIIAINKIDLPDS
                     NVDRVVAQLLDYGVFLEQYGGDVPWCKTSTVTGEGILNLIEIIILQSELMELKAPVEV
                     PAEAVVIESQKDTRMGSVATILMQEGRLSRGDIVVCGATHGRVRRMENERGQELKILG
                     PSDVARIYGLNETPKAGDMLNQVDNEKTARGISSERQQIRLEREKFQNKSSLQNIFAR
                     IQEDQINDLKIIIKTDTDGSAEALADSFQKLSNSEVQVNIIHKSVGGINEADVSLASA
                     SDAVIIGFHVRASQQARKLAEDEGVEIKLYQVIYDAIEEIRSSLEGMLKPGFEEQILG
                     AATIKQVFRIKKVGTIAGCQVDRGVIRRNCKVRLYRNDVLVTEDDLASLKHYADEVDE
                     IRAGTDCGLSLKSFTDIKEGDVLECYIINEVIKKL"
     gene            5501..5980
                     /gene="rbfA"
                     /locus_tag="PLO35_02860"
     CDS             5501..5980
                     /gene="rbfA"
                     /locus_tag="PLO35_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOH78635.1"
                     /translation="MKSFRVPRLQEELKKMFNIILSQKLRDPKLAWVNVTDVVISKDL
                     RYAKVYFSHYNNPGDHQEIRDLLYKTSGFFKKQIAGAHLMRTIPELSFYYDDTEDRAA
                     KVDALLTRIKDNYDDEDDSDYDPDIDIDDFLDDDDYYDIDEEEDYEDEFDDDDEEEE"
     gene            6103..7101
                     /locus_tag="PLO35_02865"
     CDS             6103..7101
                     /locus_tag="PLO35_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH78636.1"
                     /translation="MDYLKQALKDLADCDDQIAIVSHVNPDGDGFCASLFLAKWLKLH
                     GRNAVIVTDGDDLERFAHLLGDARIVIYDESLSFSRLVVMDCNSMVRLGRRGELVAKA
                     TSVLLIDHHEVEHGLIPAQISFIDQSYVCVGAILYAALEDELISLEPASRQYLAACLY
                     TTLLNDTNNFTNGNTDAGVLTLAGKISALGIRPHLLYRAYFQNQSYREMRYTGEVLST
                     ITIHHGGRVLLLWSSLEMAKRNDIDPGAVLNVTRWVQGVKDIDAIVFIREEQPGFHKL
                     SFRSMKVDVNRFAARYGGGGHRNASGCHLNGELEQIIAMVMGDFGKALEEADSTDA"
     gene            7094..7969
                     /gene="truB"
                     /locus_tag="PLO35_02870"
     CDS             7094..7969
                     /gene="truB"
                     /locus_tag="PLO35_02870"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOH78637.1"
                     /translation="MPDKGFLLIDKTAGITSFDVIRRLRKLTGIRRIGHCGTLDPFAT
                     GLLICALGSYTRLIGYLEALDKSYEATVLLGKSTSTGDPEGEINQISPVNTDGIDPEN
                     LKAAALALKELPIPIYSAVKISGKRAYTLARAGEQVILDARPTTIHDFRVTSQPGPDD
                     PEPALGYRCTVSKGTYIRSLSEWIARQLDTVGYTTSLKRVAIGAIDLNEAISLEALTP
                     DNWADYLCSPLRLFGHLEIRPTSSEELVILARGQSLSDDAEDNPTVLIGGSGNDLAGV
                     AERSEGKLHPRINLR"
     gene            7966..8865
                     /locus_tag="PLO35_02875"
     CDS             7966..8865
                     /locus_tag="PLO35_02875"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOH78638.1"
                     /translation="MSCILSIGNFDGVHLGHRELLRGMVSLAKSLQLPSVVITYTDHP
                     AFVLNKRPRPLLLTPSSRKAQLLKELGVDQVRLIPFTQEFARTSAADFLKDFLIPEYH
                     PHTIVVGYDSHFGHAREGTLAFLKQHASIYGYRCRYQKPFMDDGSVVSSSRIRASIKE
                     GRLEEANRMLGTPYKLYGKVVGGQGLGRGLGFPTANLGLDDPHQLVPRAGVYLSRTLL
                     SGQAFFGLTNIGTSPTLKHTDETVVETYMLDYDQEIYGAALELELLSYLRPEETFDSV
                     EALKLAMHNDLSRARAMIGGMPE"
     gene            8862..10760
                     /locus_tag="PLO35_02880"
     CDS             8862..10760
                     /locus_tag="PLO35_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78639.1"
                     /translation="MRLTLTVTLLLMLVSLTAISYHPGSGRIVDTSQRAFHLAYHTSS
                     DDLHFYGSADWAVRFDFANYYLPNLTERSFRIDALRLWFPQTGDSVKVKLMTDVDGQP
                     GLPQLADLRVPVGTNQLVLTLPEPVTVQLVWLLVEYQTNLANRFVPASRGGGTRSYYL
                     NTSMGTPYYQSFANAGFAAELLFGVEGEFIDVNDLRLMDLSLEGEIEPGATVYPSFSI
                     HNHSGQTLNSASLQLNLLTPDQDANQTLIIPIPGGIAPYGDFVWDASQPSYLDQAITL
                     PGQAMQMRLRGTLSGGPPSDVPGNNVIDRYYYTFIDTPPVHLVENFIRLSHIATVITL
                     EDQVLGDDQFRNVHPLDYYPVLADSLSAPGAMARFSWYGSNATPLVVLGGTRRIPGFS
                     AAFASDFTADSADLITDKSFISAGAVTSNFSGDSVDFRFSFENTQTSLLSGTGVYDLV
                     AGSVLCAGVFKHVIVGGMETWVFDRWIAHALPLTQGLNLGETLEVELSIPRTGLEVTS
                     QYRTYYWIQVAGGGRVLWAGFQDLVIIPSSANDELLPAPGLKAAPNPLRGRAELTIEL
                     AGVSAKAQNHLRIYNLRGQLVFKTTLKGEKIQLPAAVFPASGIYFIQIKGLAGQKDPL
                     QARITVIK"
     gene            10897..11847
                     /locus_tag="PLO35_02885"
     CDS             10897..11847
                     /locus_tag="PLO35_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH78640.1"
                     /translation="MVQHLFINELQDQQGKEITAYYLVAEKEIREGKNGQFLRLRAQD
                     RSGSVTGYMWKDFQKLSEDFSEGDVVKIRASVQNYKGQIQLNISQLRFADHSEYDPDI
                     YLTRSKIPPEILSERFFSFVDKVENTFLNRLLRDIFDDKEFFNHFLKAPAAKSWHHNY
                     IHGLIEHTVSVASICEFVSSMYPVNQDLLLSGALLHDAAKVLEYTSKPPIDFTDLGRL
                     IGHLSLSDQMICEKARAIPGFPDALLMHLRHLVLAHHGEYEKASVRLPQTLEAQVLHL
                     CDNMDAQSVGVTQLIEAAPKDAEWTEFDKLNSRYYKLTKF"
     assembly_gap    11872..11971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11972..12781
                     /locus_tag="PLO35_02890"
     CDS             <11972..12781
                     /locus_tag="PLO35_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH78641.1"
                     /translation="CAASHIRNMFQTSVLVSGSKGNSVLVRTGDTALLLDAGVAAKTI
                     FAAMDALRVPRESIQGIVVSHEHSDHIRGVGVVARALKIPVFINEDTYCYCSERLGKL
                     PWEPVFFETGQSFQIGDIIVHPFSSSHDAVDSCNFTFRRTEDEERRLGVATDLGYPTN
                     LSIENLKLCTTLVLESNHDLQMLMNGPYEWHLKQRVKSRLGHLSNDQAVGLISNVMHH
                     GLDNLVLAHLSEINNHPDLAIGAMRDYLSSLRRDLRLLVASQYEHTPLIDV"
     gene            13236..>17797
                     /locus_tag="PLO35_02895"
     CDS             13236..>17797
                     /locus_tag="PLO35_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH78642.1"
                     /translation="MKKALVFLITVLGILSLAAQSNMFLQLSEEQITPEIYEQIERNN
                     AQVRGPSFNFVSTPYNLPDNADIADPLTGRFNEDTPWTKDFSRHVVNPEGNQLYFYFS
                     NTEHFIITQPDSGNPLKLRFQPIPTHWYGSELMVITVSDEPLDRTNRATATAIIRINV
                     SSVPDPPLFVNLPAGNIFTIDEDASLSVNFRDYINCIDSSIENFDLYVMQTQAPIGPE
                     TYNVMVSQAPNIYTGHQVTFTPQPDYNNPIGVRFIVTAVDRGSSAFSTVEIIVRIIPE
                     NDPPQIVSYLPANLEFTIDQNTTQAFSAVTQDADNDVLAHEWLLEGLLNGIPFSNVVS
                     TNADLSYLFDVPGTYTLTYTVSDTWTQDSIQWTIHVRPIGPLFNPVGGVFDHAITVTL
                     TAPAGFEAAIIYYTLDGSLPVPGAPGTFVYDQPIPLETLGNIENIKTIRALYVHPDFP
                     PSQVVSYNYRITGQVAELVFNPVGGTYYSQVAVNITCPNAGATIWYTTDGSDPIPGDP
                     DTFEYTGPIVIPTGNSMVIKAMATRADWIDSPTRTEAYNVTGVVTIVSHTMTPPPLPD
                     GEFYTVELGESMPVEILGLTLLPPSATLYYTLDSSIPGPTNPAALIYTPGLEIPLTCP
                     TWITFRAYNPDWLPSTIYTYFYDVRTRTKIVPFVNGTVFDPVPGTYTAPVMVSLSLET
                     VPANAPVYYTTDGTDPIDDPALLYNGPIDVSSATTIKVIARDPSICPSEIYTGHYVIT
                     GQLEMPQFSPPPSSYQVPEPITIYHDRPGVTIYYTLNGDDPDEFANLYSAPINLPSGT
                     HTIKARAYLADWIPSNIRTGVYRFGYLPVPVFNLEGGIYTAPIVVELSSTVAGASILY
                     TTTVGGVPSTLYNTGIPVGNLQPETVTIRAKATRAGWADSDVVEREFTVTGTVAMPSF
                     NPPGSDGPFNGTVNVTIATATPGAQIKYTLDGQDPSESYGIVYAGPVAIAQSATLKAR
                     AFLADWAPSAIRTDVYTILGDIAMPVFTPAAGTYTGPVNVFISVNPPDAQIYYTADGT
                     IPTAATGIPYVAGTPIPVSGTSLLQAIAVKPGWNDSPIATALYTITGTVAAPVIAPVS
                     GTYYSTQTITITSATPGATIVYTTDGTEPSRLNGLPYARPFNLGAGVWAVKAMAWLDT
                     WADSPVTVNNYVIIINPPIANPVFSPPGGYYNDPVDVTITAAPADATIYYTLDGTIPS
                     LSNWTAYSGPISVNAYTQIKAIAIRANWPNSGVSTADYYFRVANPVLSVPSGAYDSPQ
                     SVSMSVTTPGALIRYTTDGSDPSGVDGTLYDGTPVLISNTTQATTVKAIAYLGGWVNS
                     SIVTYTYVINPPVADPFFSVQGGDYYAAFSVAMTTLPSIASIYYTTDGSDPSEVNGTL
                     YMGPVAITENTTLKARAYMVNYPPSGIRTAVYNLFARPVSFDPPGGTYNANQNVTLST
                     VTPGATIWYTTDGSDPIPGVSSVFDPLNPVLVDHNLTIKAIAGLNNWFSSPITSASYI
                     IDIPLPNIAVVQILPRSGVYTAPVDV"
     assembly_gap    17798..17897
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17953..>20045
                     /locus_tag="PLO35_02900"
     CDS             17953..>20045
                     /locus_tag="PLO35_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH78643.1"
                     /translation="MNRSLISNASYVINIIIPNVEAPVFSLVSGLYYAPIDVAITTGT
                     ADAEIWYSFDGSDPTPEAAGSMRYSGAINIPGNSTRFIKARAYKTGWNTSPVVSANYN
                     VTGTVADIQYSVLSGTYTTPQSLVLTTSTQDATIRYTTDGSDPTATSTPYITAIPINL
                     SMTVKARGYKTNWLPGNPGMEVYVITGSLAFDQPSLSPAPGTFGNAISVTIANPIPPD
                     ANVYYTTDGTVPSATNGTLYSGPIAVNAALTLKAVALKAGWVPAYLDGTYGFQAATPG
                     FLPGSGAYPNAQLVSLSTSTDGASIRYTTDGTDPSALNGSVYSAPISVEVNQTIKAYA
                     FKPGYLDSPIVAATYAIGTYIPVVDTPVLNPASGTYTETRSVAMSVGTPGAVIRYTTD
                     GSEPTEASDEYTAPVSLALNTVTTIKAKAFKVDWMPSATTTELYVITGQVAAPMFIPP
                     AGSYTAAQNVVLTSATEGAFFRYTLDGSDPVAGSPLYTTPINVPQNLITTIKARAYKS
                     GWADSDVSTAVYNVTGKVAFTPPAFTPPSGTYATGQMINISAPVPASATVKYTIDGTL
                     PSRTNGIEYTAPFSLATGATVQAIAYLDGWEDSQVVSAQYNFNVAAPVYTPQSGTYPS
                     LQVTIGSATPGSQIRYTMDGSDPSDTVGTLYTGPIDLAGNNSYQFKAIAYLAGWQNST
                     IVPATYLVTGTVADVV"
BASE COUNT         4786 a         5799 c         5109 g         4151 t
ORIGIN      
        1 cagatcttcc cttttagcca cctatactcc acctatgcct ctcctttacc tctcctttgc
       61 tttggcacag gtggagggga ggaggtgcag aggagcagca aagcctgctg gaagggcatc
      121 ttcggggtgt aacaaaagca tggctgtcaa tggaaccggg ttgcaaacca gtacctgagg
      181 ccgcaaacag cctttcgggc agatttagct tgacacatat ccaaggccgg agattttgac
      241 gcaactagtc aagacggcag catagccgtc agccagagaa tagtttgccg ccgcgcggct
      301 ccggatagct acccatggtt agctattttt tttgatgtaa aacgcttatg aaataaaggg
      361 aaacacaatg gaatactatc gggtacagat cgaagaaatc gccaagcgta tctgcagcga
      421 gaagcatttg gccctctacg acatcgatga aaagatgagc ggcaagggcc gcatcatcac
      481 ggtgtacctc accaaaatcg gtggcgtcac cctggatgag tgtgccagct tcagtcgcgc
      541 tttgggcgcg gaactcgagg agtttgactt gatcccggag cgttattacc tggaagtttc
      601 ttctcccggc gtggaacgtc ccctcaagct taaaagccac tatgtgagcg ccatcaacga
      661 aaaggtggcg gtccagttca acgacggcga agaaaagaag agcgtgatgg gtacgctcac
      721 ggtggtagat caggatgaga tcaccattga tgaccggggc actttgcacc tgatcccttt
      781 caagcagatc agtcgtgcca aaaccgtgta tatacccgat tggagtgaga cagatgagcg
      841 ctaacatatt ggacgcggtg atcaagcttg ccgccatcaa acaactggac aaagaccaga
      901 ttcagcggat catagaagaa gctatcttca gcaccttgga aaagcgtctg ggtcccgata
      961 acgaactgac tgtcttcgcg gaccaggcca ccggaaaaat ccaggccagg tttttgtgcc
     1021 gggtggtgga actggaagag aatctgggcg aaatctccct ggccgaagcc cgcaccctca
     1081 acccttccgt tcagcttgag gacatgatcc agcgcagtat gtcgctgtat gaatttgagc
     1141 ccaagctggt gaaaaacgcg cagcgcctga tcatggaaaa gatcaaagaa ctggaagacg
     1201 agaagatcca gaaagactac aacaagctca aaaacaccat cgtggtgggc aagatcaaga
     1261 ccgtggatga aaacggcggc taccgcatcg acatcaacca caccgacgcg ctgcttcccc
     1321 ccgacgaaca gatagaaaac gaatactatc gcgtggggga aaacatccgc gcctacgtgg
     1381 tcaatatccg ctctcatcaa tccaacatca ccatcatcct ctcgcgcacc aatcccgagt
     1441 ttgtcaaaaa gctctttgag gctgagattc cagagattta cagcggtcag atcaccatcc
     1501 gcaagatagt ccgcgaacct gggatccgca ccaaagtaga gctggaaacc aaagatccca
     1561 agctggaccc catcagcgcc tgcgtgggga tcagaggtac caggatagat ggcatcaggc
     1621 gtgagctgca cggcgagcag atcgacatcg tggtccattc cgatgacccc gaacagatga
     1681 tcatcaacgc cctcggtatc gatggcgcca tcaatgtgat cattgaacgc aaccgggccg
     1741 ccagcgtgat cgtcaacgaa gataacaagg tgatcgccat cggtaagcag ggtaaaaacg
     1801 tcaagctggc cgccaagctc accgggatga aaatagatat ctttaccagc gctgaatttg
     1861 atgaaaagat ggccaaggag cgccgcaccg tgagccatat cacagaattg gacggagtga
     1921 cggccaagat cgccgaagcg ctcaaacaag ccggctacac ttccgtgcag gacgtcttcg
     1981 cggcctcact ggacgagctg gtcaacctgg aaggagtcgg tcagaaaacc gctgaacgcc
     2041 tcaaggaagc ggcaaagtat ttctaatgcc aaaccataac agcaaagccg gacaccaagc
     2101 tcagcggacc tgcgtggtct gccggggcaa aaacgccctg gacggcatgc tgagctttat
     2161 cctgctggat ggcaaactgg tctttgatct gggtggagcg ttgcaatgcc gcaagtttca
     2221 cctctgtccc ggacccgaat gtgtggaagg cctgcctgtg tggatcaagc gttatcacaa
     2281 aaagcgggct ggatcaggat cccggagagg ccagaaatga cggctacccc caaaaaacat
     2341 ccccggccag ccaggagctc cagcgctccc agaggggctg gaagccaacc cggagccaaa
     2401 aagctccctg cgccccggcc gcgtgtgact ccagcccagg cctcggattc cctgcctgtg
     2461 aaagccaggg cccggcaaac gattgcggtt acggccccgc aactaaaaaa aatgaccacc
     2521 ctgatgcagt ttgcccgccg agccggcaag ctgatttcag gttatgacgc ctgcctgcgt
     2581 gccatgcatc aacacaagct gtatctggtg ctgctcgctg aagatacggc cccgcggact
     2641 tgtcggggca tagatcacgc ccttaatgaa aaccagatcg ccatcccgct catgcgggcc
     2701 ggcaatcaaa ccgatctcag ccaggctttg ggcttaccca aaagcggcgt tttcgggatt
     2761 ggggataaac aattcgcgaa agccatccag gcctgtgccg ggatggaacc gcgacgggag
     2821 gaaccttgca aataagagta catgaactgg ccaaggaact caaaataagc actatggccc
     2881 tcaagaaaca cctcattgac cttggggtgg ttacaaagag ccatatgagc tttattgaag
     2941 aagacgttgc ggacaagatc cgcagaaaat acaacgagca ggtggacgcg gagaagcgtg
     3001 ctgaaaaaga acgcaagaag tttatcgaga tgcgccaggc cgccaaacac aagcatgagg
     3061 acatcgtgat cccggaaccg gtgaaagagc cggaacccga ggctgctcca gctgccaggc
     3121 ccaccgcgaa gaccaaagcc gctccggctg aggtttccga agaacatgct tcttctgagc
     3181 cctccgaagc tccagaccca aagcaggaag cggaaccgga agtcccggtg accagggaag
     3241 agcctgtcac cgtgccggaa gctcccgtca aaccagttcc cgaagctccc aaacaccgga
     3301 tcatcgttcc ctacacccag gctacgccag ttcagccacg cggtcccaga gagcgggtca
     3361 ggcccaaatc ttatctggag actcagacgg cgagaagtgg tcccaaaccc cccgcgagcg
     3421 gacgtcccag tcccggcacc agaccacgcc ccgattccag aaagcctggc gaaagcctgc
     3481 cggacgtcaa gcccctgcct gtcactccag ccgcggccaa agaagccgaa gaaaagaaat
     3541 tcggcaaagc caaggtcagt catgaggaat tgggagacaa gagcaagcat aaaaaagcca
     3601 ttatcaccag taccaagaag acgaaacaga aggtagccga gcccacagaa gtggatgagg
     3661 ccgccatttc cagaaatatc aaaaagaccc tccagaaaag ctccaagcgc aagaagtatc
     3721 atcgtgaagc tcaggctgtc gataccagtt ccacggagat cgtgatcagg gaatacacct
     3781 cggtgagcga actggccaag atcatgaacg tttccgcggc cgagatcatc tccaaattct
     3841 ttatgatggg ccagttggtg accatgaacc agcgtctgga caaggactcg ctggagatga
     3901 tctgtgatga gtttaaggtg gaatttcgct ttgaagatga gtatggcgct gatatcatcg
     3961 acagggaacg tgaaagctat caggatatag aagccgaaac ccgtgccccg gtggtgacca
     4021 tcatgggcca cgtcgatcac ggcaagacct ccattctgga ctatatccgc aacaccaata
     4081 tcgtggctgg cgaatcgggt ggcatcactc agcacatcgg tgcttaccag atcgaaatca
     4141 acaagcacaa gatcaccttt ctcgatactc ccggtcacga agccttcacg gcaatgcgtg
     4201 cccgcggggc caacgttaca gacatcgcgg tgatcgtggt ggcggccact gagggcatga
     4261 aacctcagac caaggaagcc atcgaccacg ccaaggccgc cggtgtccag atcattattg
     4321 ccatcaacaa gatagatctt ccggattcca acgtggaccg cgtggtggcc caactgctgg
     4381 attatggagt gtttttggag cagtatggtg gagacgtgcc ttggtgcaag acgtccaccg
     4441 tcaccggaga aggcattctc aacctcatcg agatcatcat cctgcagtcc gaactgatgg
     4501 agctcaaagc ccccgtggaa gttccggcgg aagcggtggt gatagaatcc caaaaggata
     4561 cccgcatggg ttcggtggcc acgatcctga tgcaggaagg cagattgagc cgcggtgata
     4621 tcgtggtctg cggagctacc cacggccgcg ttcgcagaat ggaaaatgag cgcggacagg
     4681 agctcaagat cctgggcccc tcagacgtag ccaggatcta tggcctcaac gagaccccca
     4741 aggctggcga catgctcaat caggtcgata acgaaaagac cgccagaggg atcagctccg
     4801 agcgtcaaca aatccgcctc gaacgcgaga agttccaaaa caaatcatcc ctccagaata
     4861 tctttgcccg catccaggaa gatcagatca acgatctcaa gatcattata aaaaccgaca
     4921 ccgacggttc cgccgaagcc ctggcggatt ccttccagaa gctctccaat tctgaggtgc
     4981 aggtcaatat catccacaag agcgtgggcg gtatcaacga ggccgatgtg agtctcgctt
     5041 ccgcttcgga cgctgtgatc atcggcttcc atgtccgtgc cagtcagcaa gcccgcaaac
     5101 ttgccgagga cgaaggcgtg gagatcaagc tctatcaggt gatctacgac gctatcgaag
     5161 agatcagatc ctccctggaa ggcatgctca aaccgggatt tgaagagcag atcctcggcg
     5221 ctgccaccat caagcaagtg ttcaggatca agaaagtggg caccattgcc ggctgccagg
     5281 tggatcgcgg tgtaatccgc agaaactgca aggtaaggct ttaccgcaac gacgtcctgg
     5341 tcacagaaga cgatctggcc tccctcaaac actatgccga tgaagttgac gagattcgcg
     5401 ccggcaccga ttgcgggctc tctctcaaat cctttaccga catcaaggaa ggcgacgtgc
     5461 tggagtgcta catcatcaac gaggtcatca agaaactctg atgaagtcat tccgcgttcc
     5521 ccggctgcag gaagagctga agaagatgtt caacatcatc ctcagccaga agctgcgcga
     5581 tcccaaactg gcctgggtaa acgtgacaga tgtggtgatt tccaaagacc tgcgctatgc
     5641 caaagtgtat ttctctcatt acaacaatcc cggagaccat caggagatcc gggatttgct
     5701 ctataaaaca tcaggttttt tcaagaaaca aatagcagga gcacatctca tgagaaccat
     5761 acctgagctc tcattctact acgacgacac cgaagaccgc gccgccaaag tggatgccct
     5821 tcttaccaga atcaaagaca actacgacga cgaagatgat tctgattacg atccggacat
     5881 cgacatcgat gatttcctcg atgatgacga ctactacgac atcgatgagg aagaagacta
     5941 cgaagatgaa ttcgatgacg acgatgagga agaggaatag ccaatcggcg agttcccaat
     6001 cactcaggct gcattaaacc attatccgcg aatggagacc tttgcgtctc cgtttacatg
     6061 gacacgaaat cgtcggcaac ggcgatttct ttttggggta agatggatta cttaaaacaa
     6121 gccctcaaag acctggcgga ctgcgatgat cagatcgcca tcgtctctca cgtcaacccg
     6181 gacggggatg gcttttgcgc ctcgctgttc ctggccaaat ggctcaaact gcacggccgg
     6241 aatgcggtca tagtgaccga tggtgacgat ctggaacgct ttgcccatct gttgggagat
     6301 gccaggatcg tcatttatga tgaatccctg agcttttcca gattggttgt gatggactgc
     6361 aacagcatgg tccgtctggg caggagggga gagctggtag ccaaagcgac aagcgtgttg
     6421 ctgatcgatc atcatgaagt ggaacacggt ctgatccccg ctcagatcag ctttatagat
     6481 caatcctacg tctgcgtggg agccatcctc tacgccgctc tggaggatga gctcatctct
     6541 ttggagccag ccagccgtca gtatctggcc gcctgtctgt acaccactct gctcaatgac
     6601 accaacaact tcaccaacgg caataccgat gccggggtac tcaccctggc cggcaagatc
     6661 agcgctctgg gcatccgtcc tcacctcttg tatcgcgctt actttcaaaa ccagagctat
     6721 cgggaaatgc gctacaccgg agaagtgcta tccaccatta ccatccatca tggcggtagg
     6781 gtgctgctct tgtggtccag cctggaaatg gccaaacgca atgacatcga tccgggtgcc
     6841 gtgctcaatg tgacccgctg ggtgcagggc gtcaaagata tcgacgcgat tgtcttcatc
     6901 cgcgaggagc aacccggatt tcacaagctc agcttccgtt ccatgaaagt ggacgtcaac
     6961 cgcttcgcgg cccgttatgg aggaggcggt catcgcaacg cttcaggatg ccacctgaac
     7021 ggggaattgg agcagatcat cgccatggtg atgggagatt ttggcaaagc cctggaggaa
     7081 gccgattcca ccgatgcctg acaagggatt tctgctgatc gataaaacag ccgggatcac
     7141 gtctttcgat gtgatcagac gcctgcgcaa gctcactggc atcagacgca taggccactg
     7201 cgggaccctg gaccccttcg ccacgggact gctgatctgc gccttgggca gctacaccag
     7261 gttgatcggc tatctggaag cgctcgacaa gagctatgaa gccaccgtcc tgctgggaaa
     7321 aagcaccagc accggcgatc cggaaggcga gatcaatcag atcagccctg taaatacaga
     7381 cggcattgat cctgaaaatc tgaaggcggc ggcactggcg ctgaaagaac ttcccatccc
     7441 gatttattca gccgtaaaga taagcggcaa aagggcttac acgctggcgc gggctggaga
     7501 acaggtgatt ctggatgcca ggcccaccac catccacgat tttcgcgtga ccagccagcc
     7561 gggacctgat gaccctgaac cagcacttgg ctaccgctgc accgtgtcca agggcaccta
     7621 catccgcagc ctcagcgagt ggatagcccg ccagctggac acggtgggct acaccaccag
     7681 tctgaaaagg gtggcgatcg gagccatcga tctgaacgag gcgatctctc tggaagcgct
     7741 cactcccgat aattgggctg attacctttg ctcacccctg cggctgttcg gccacctgga
     7801 gatccgtccc accagcagtg aagaactggt catcctggcg aggggacaaa gcctcagcga
     7861 cgatgctgag gacaacccca ccgtcctgat cgggggatcc ggcaacgatc tggctggagt
     7921 ggcagaacgc agtgaaggca agctgcatcc caggataaat ctcagatgag ctgcattctc
     7981 agcatcggta attttgacgg ggtccatctg ggccaccgtg agctgctgcg cggcatggta
     8041 tcactggcca aaagcctgca actgccttcc gtggttatca cttacaccga tcatccggcc
     8101 ttcgtgctga acaagcgtcc acgacccctg ctgctcactc cttccagccg taaagcacag
     8161 ctgctcaagg aactgggagt agatcaggtg cggttgatac cctttaccca ggagtttgcg
     8221 cggaccagcg cggcagattt tctgaaggac tttctgatcc cggagtatca tccccacacg
     8281 atcgtcgtag gctacgactc ccattttggt catgccagag aaggcaccct ggcctttctc
     8341 aaacagcatg cctccatcta cggctaccga tgtcggtacc aaaagccatt tatggacgat
     8401 gggtcggtgg tgagcagcag cagaatcaga gcaagcatca aggaaggacg cctggaagag
     8461 gccaaccgga tgctgggaac cccttacaaa ttgtatggaa aggttgtcgg cggacagggt
     8521 ttgggcagag gcctgggctt tccaactgcc aatctgggcc tggatgatcc ccaccagctg
     8581 gtcccccgcg cgggtgtata tctgtccagg actctgctga gcggtcaggc gttttttgga
     8641 ttgacaaata tcggcaccag tcccaccttg aaacacaccg atgaaacggt ggtggagacc
     8701 tatatgctgg attatgatca agagatatac ggcgcggccc tggagctgga actgctcagc
     8761 tatctgcgcc cggaagagac tttcgacagc gtggaagcgc tcaaactggc tatgcacaac
     8821 gatctgtcca gggccagagc aatgatcggc gggatgcctg aatgaggctg actctcactg
     8881 tcacgctcct gctgatgctt gtcagtctga cggcgatcag ctatcatccc ggcagcggca
     8941 ggattgtcga cacgtcccag cgggcctttc atctggccta ccacacttcc tcggacgacc
     9001 tgcattttta tggcagcgcg gattgggcgg tgcgctttga ctttgccaac tactatctcc
     9061 ccaatctgac ggagcgcagc ttcaggattg acgctctcag gttgtggttt ccccagaccg
     9121 gcgactctgt aaaggtcaag ctcatgaccg atgtggacgg ccagccggga ctgcctcaac
     9181 tggcggacct gcgagttcct gtgggtacca atcagctggt gctgacgctc ccagagccgg
     9241 ttaccgtgca actggtctgg ctgctggtgg aatatcagac caatcttgcc aatcgcttcg
     9301 taccagcctc acggggaggg ggtactcgga gctattatct caacaccagc atgggaaccc
     9361 cctactatca gagctttgcg aacgcgggtt ttgcggcaga gctcttattt ggcgtggaag
     9421 gtgaatttat cgatgtcaac gatctgcgcc tcatggacct gtccctggag ggagagattg
     9481 aacccggggc cacagtgtat cccagcttca gcattcacaa tcacagcggc cagaccttga
     9541 attccgcgtc cctgcaactg aatctgctca ctcccgatca ggatgccaac cagactctta
     9601 tcatcccaat cccgggaggg atcgctccct atggagactt tgtctgggat gccagccaac
     9661 cctcttatct tgaccaggcg atcactttac cgggacaagc gatgcaaatg cgcctgcgtg
     9721 gaactctgag tggcggtccg ccctcggacg tccctggcaa caatgtcatc gacagatatt
     9781 actacacctt catagatact cctccggtcc accttgtcga aaacttcatc cgcctgtccc
     9841 acatcgcgac ggtcatcacg ctggaagatc aagtcctggg tgatgatcaa ttccgcaatg
     9901 tccatcctct ggactattat cccgtcctgg cggattctct ttccgcacca ggagcgatgg
     9961 cccgtttcag ttggtatgga tcaaacgcca cgcctctggt cgtgctggga gggacgcgtc
    10021 ggattcctgg tttcagtgcg gctttcgcga gcgatttcac ggccgacagc gctgatctga
    10081 tcacagacaa aagcttcatc agcgcggggg cggtgaccag taacttttcc ggtgacagcg
    10141 tggattttcg ctttagtttt gaaaacactc agaccagctt gctgagcggc acgggagtgt
    10201 atgatctggt ggcgggaagc gtgctctgtg ccggagtgtt taaacatgtg attgtgggcg
    10261 ggatggaaac ctgggtgttt gatcgctgga tagctcacgc tctgccgctc acccagggcc
    10321 tcaacctcgg tgagactctg gaagtggagc tttcgatccc caggaccggc cttgaagtca
    10381 cctctcaata ccggacctac tactggattc aagtggcggg gggaggaaga gtgttatggg
    10441 ccggatttca ggacctggtc atcatcccct cctcagcaaa cgatgaactg ctgcccgcgc
    10501 caggtttgaa agcggctccc aatcccttgc ggggacgggc tgagctgact attgaactgg
    10561 caggcgtaag cgccaaagct cagaaccatc tcagaatcta caaccttcgg gggcagttgg
    10621 tcttcaagac caccctcaag ggtgaaaaaa tccaactgcc ggctgccgtg tttccggctt
    10681 caggcatata ttttattcag ataaaagggc ttgcgggcca aaaagaccct ctgcaggcca
    10741 ggatcacagt gatcaaatga tgaaaccgcg tcaatccacc ccagtgaggc ctcaaagctc
    10801 tcataacgga gtggagtgtt tattcaggct ggaaagccat ccggtccggc cgtgggactg
    10861 taaaggtccc caaaccccca atcaatgaag gaaattatgg tacagcactt atttataaat
    10921 gaactacagg accagcaggg caaggagatc acggcctact acctggtggc ggaaaaagaa
    10981 atccgcgaag gcaagaatgg tcagttcctg aggctgagag cccaggaccg cagcggttcc
    11041 gtgaccggct atatgtggaa ggatttccag aaactcagtg aagacttttc ggaaggcgac
    11101 gtggtcaaaa tccgcgccag cgtgcagaac tacaaggggc agattcagct caatatctct
    11161 cagttgcgct ttgccgatca ctctgagtat gatccggata tctatctcac ccgcagcaag
    11221 atacctccgg aaatcctctc cgagcgcttc ttcagctttg tggacaaggt tgagaatacc
    11281 ttcctgaaca gactgctcag agacatcttc gatgacaagg aattcttcaa ccacttcctc
    11341 aaagctccgg ctgccaaatc atggcatcac aactatatcc atgggctgat cgagcatacc
    11401 gtgagcgtag cctcgatctg cgaatttgtg agcagcatgt atcccgtcaa tcaggacctc
    11461 ttgctcagcg gcgctctgtt gcatgacgcg gctaaggtgt tggaatatac cagcaagccc
    11521 cccattgatt ttactgatct gggacgcttg atcggccacc tcagcctctc cgaccagatg
    11581 atctgtgaaa aagcccgcgc cataccggga tttccggatg cgctgctgat gcatctgcgc
    11641 catctggtcc tggctcacca cggtgagtat gaaaaagcct cggtgcggct gcctcagacc
    11701 ctggaagccc aggtcctgca cctctgtgac aacatggacg cccaaagcgt tggggtgacc
    11761 cagctcatcg aagccgctcc caaagacgcc gagtggactg aatttgacaa gctcaacagc
    11821 cgctattaca aactcactaa gttttagggt tttaaagttt tagagtttta gnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgcgcagca tctcacatac gtaacatgtt
    12001 tcagacctct gtcctggtga gtggttccaa gggtaattcc gtgttggtca ggaccggtga
    12061 cacggccctg ctcctggatg ccggagtggc cgcgaagacg atctttgcgg cgatggacgc
    12121 tttacgggta ccgcgcgaaa gcatccaggg catcgtggta agtcacgagc actcagatca
    12181 tatccgcgga gtgggggtag tggcacgggc cctgaaaatc cctgtgttta tcaacgaaga
    12241 tacctattgt tactgcagcg agaggctggg caaacttccc tgggagccag ttttcttcga
    12301 gacgggccag agcttccaga tcggagatat catcgtgcac ccgttttcat cctcacacga
    12361 cgcggtcgac agttgtaact tcacttttcg caggacggaa gatgaggagc gtcgtttggg
    12421 agtggccacc gatctgggat atcccaccaa tctcagtatc gaaaatctga aactttgcac
    12481 taccctggtg ctggaaagca atcacgacct gcagatgctg atgaacggtc cctatgaatg
    12541 gcatctgaaa cagagggtaa aaagccgcct gggacatctt tccaacgatc aggccgtggg
    12601 cctcatcagc aatgtcatgc atcacgggct cgacaacctg gtgctggcac atctatccga
    12661 aatcaacaat catcccgatc tggccatagg cgccatgcgg gattatctct ccagtctcag
    12721 aagagatctc agattactcg tggccagcca gtatgaacat acccctctga tcgatgtgtg
    12781 aaaacctggc ttgatgggcc agccagcagc tttccaaccc tctgatccag ccaagcgaaa
    12841 ctgaattttg cctattcctt gccgattcgc attacggtgt tattacggac tcattacgga
    12901 cttgacccgt aatgagtccg taataggatg cggggaggag gaggcaacag ctatccatgt
    12961 tttcagtctc aatactgagg ttgaaaatat ctgtgtctat gatattggga gaaatgggta
    13021 gtcaggggta atttttaccc aatccttccc gactggcccc aagtaaaaaa gtagttgaca
    13081 gcaaggcggg tagctttatt atggcatcat gacgaatata agttttcgat tttttggcaa
    13141 gcccatgagg gcttcatcat atgtatacca taacatcttc gtggttagac acttgagggt
    13201 gttgcatcac tttagaacca gataggagaa agcacatgaa aaaggcatta gtatttttaa
    13261 tcacggtttt aggcatctta tctctggctg cccagtcaaa catgtttttg cagctatcgg
    13321 aagagcaaat tactcctgag atctacgaac aaattgagcg caacaatgcc caggttcggg
    13381 gtccctcctt caactttgtt tctacgccct acaaccttcc ggacaacgct gatatcgctg
    13441 atccgctcac gggccgtttt aatgaggaca ctccctggac taaggacttt agccggcacg
    13501 tggtcaatcc ggaaggtaac cagctctatt tctattttag caacaccgag cactttatca
    13561 tcacccagcc agatagcggc aatcccctca aactgagatt ccagcccatt cccacgcact
    13621 ggtatggatc tgaattgatg gtgatcactg tttccgatga acctctggac aggaccaacc
    13681 gggctaccgc gacggccatc atcagaatca acgtgagctc cgtgccagac ccgcccctgt
    13741 ttgtcaacct tccggctggc aatatcttta ccatcgatga agatgcgtcg ctctcggtga
    13801 atttccggga ttatatcaat tgcatcgaca gctcgataga aaacttcgac ctctatgtga
    13861 tgcagactca ggccccgatt ggcccggaaa cctataatgt gatggtaagc caggctccca
    13921 atatctacac cggccaccag gttaccttca cccctcagcc cgattacaac aatcccatcg
    13981 gggtaaggtt tatcgtgacg gcggtggata gaggttccag cgcgttttcc accgttgaga
    14041 tcatcgtgag gattattccc gaaaacgatc ctccccaaat tgtttcctat ctgccagcca
    14101 atctggaatt taccatagac cagaatacaa ctcaggcctt cagcgcggtt acccaggacg
    14161 ccgataatga tgttctggcc catgagtggc tgctggaagg cctgctgaat ggcatcccgt
    14221 tctcaaatgt ggtttccacc aatgccgatt taagttatct ctttgacgtg cctggcacct
    14281 acactctcac ctacacggta agcgacacct ggacccagga cagcatccag tggaccatcc
    14341 acgtgcggcc gatcgggccc cttttcaatc ccgtgggggg agtgtttgat catgctatca
    14401 cggttaccct caccgctccg gccggtttcg aagcggcgat aatctactat actctggatg
    14461 gcagtctgcc tgtacctgga gctcccggca cttttgtcta tgatcagccc attcccctgg
    14521 aaactctggg gaacattgaa aacatcaaga cgatcagggc cttgtatgtc catccggatt
    14581 tcccgccctc ccaggtggtg agctataact accggatcac tggacaggtg gccgaactgg
    14641 tctttaatcc agtgggtggc acctattact ctcaggtggc cgtaaatatc acctgtccaa
    14701 acgccggcgc gacgatctgg tacaccaccg acggcagcga tcccatcccc ggcgatcccg
    14761 acacctttga atataccggg ccgattgtga ttccaaccgg aaactcaatg gtaataaaag
    14821 ccatggccac ccgcgccgat tggatagaca gccccacccg gaccgaggcc tacaacgtga
    14881 ccggggtagt gacgatcgta tcccacacca tgactcctcc gccccttccg gacggggagt
    14941 tttacactgt ggaactggga gagtccatgc ccgtggaaat tctggggctc accctcctgc
    15001 ctcccagcgc tactctgtac tacactttgg acagcagtat ccccggcccc accaatccgg
    15061 ccgctttgat ctatacgccc ggcctggaaa tcccgctcac ctgccccacc tggatcacct
    15121 tcagagcata caatccggat tggctgccca gcaccatcta cacctatttc tacgatgtgc
    15181 gcacccgcac caaaatcgtc ccctttgtaa acgggactgt ctttgatccc gtgcccggca
    15241 cctataccgc tccggtcatg gtctccctca gcctggagac cgtgccagcc aacgcgcccg
    15301 tttactacac caccgacggc accgatccaa tcgatgatcc ggccctgctt tacaacggcc
    15361 cgattgatgt gagctccgcc accacgatta aagtgatcgc ccgtgacccc agtatttgtc
    15421 ccagtgaaat ctataccgga cactatgtga tcaccggtca gcttgagatg ccgcagttct
    15481 ctcctcctcc cagctcctat caggtcccgg agcccatcac gatctatcac gacaggcccg
    15541 gcgtgaccat ctattacacc ctgaacggcg acgatcctga cgagtttgcc aacctctatt
    15601 ccgctcccat caacctgccc tccggcaccc ataccatcaa agccagggcc tatctggccg
    15661 attggattcc cagcaatatc cgcaccggcg tttaccgctt tggctatctg cccgtgcctg
    15721 ttttcaatct cgagggtggc atctataccg ctcccatcgt ggtggaactg agctcaacgg
    15781 tggcgggggc atcgatcctc tataccacta ccgtcggggg agtgccttcc actttgtata
    15841 acactggaat cccggtggga aatctgcagc ccgaaacagt caccatccgc gccaaggcca
    15901 cccgggccgg atgggcggat agcgatgtcg tggagcgtga attcacggtt accggcaccg
    15961 tggcgatgcc gagctttaac cctcccggca gtgacggtcc ctttaatggc accgtcaacg
    16021 tcacgatcgc tacggcaacg cctggggccc agatcaaata cacgctggac ggtcaggacc
    16081 cctccgagtc ctacgggatc gtgtatgccg gtccggtggc catcgctcag agcgccactc
    16141 tcaaagccag ggccttcctg gcggattggg ctccatctgc catcaggacc gatgtttaca
    16201 ccatcctcgg cgacatcgcc atgccagtct tcacacccgc cgcgggaacc tataccgggc
    16261 ccgtgaacgt gtttatctct gtcaatcctc ccgatgccca gatctattat accgcggacg
    16321 gcaccattcc aaccgccgcg actgggattc cctatgtggc aggcactccc attccggtgt
    16381 ctggcaccag tctgctacaa gcgattgccg tgaagccggg ctggaacgac tcccctatcg
    16441 cgacagcgct ctatactatc accggaacgg tggcggcacc tgtgatcgct cccgtcagtg
    16501 ggacctatta cagcacccaa accatcacga tcaccagcgc caccccgggc gctacgatcg
    16561 tctataccac cgatggaaca gagcccagcc gcctgaacgg cctcccatat gccagaccct
    16621 tcaatctggg cgcgggtgtg tgggccgtca aagctatggc ctggctggat acctgggcag
    16681 attctccggt gactgtaaac aactatgtga tcatcatcaa tcctcccatt gccaatccgg
    16741 tcttctctcc tcccggcggt tactataatg atccagtcga tgtgaccatc acagcggccc
    16801 cagcggacgc gaccatctat tatacgctgg atggaacgat tccttcccta tccaactgga
    16861 cggcatactc gggcccgatt tctgtgaatg cttacaccca gatcaaagcc attgcgatac
    16921 gcgccaactg gcccaactcc ggtgtgagca ctgccgatta ttatttcaga gtggcaaacc
    16981 cagtactgtc agtgccctcc ggggcctatg acagccctca gtctgtgagc atgagtgtga
    17041 ccactcccgg agccctgatc aggtacacca ccgacggcag cgatccttcc ggagttgatg
    17101 gtaccctcta tgacggaacc cctgtcctga tctccaacac cacccaggcc accactgtga
    17161 aggcgattgc ctatctgggc ggctgggtca attccagcat tgtcacctac acctatgtga
    17221 tcaatccacc tgtggccgat cctttcttct cagttcaggg gggagattat tacgcggcct
    17281 tcagcgtggc catgaccacc cttccatcca ttgccagcat ctattatacc accgacggca
    17341 gcgatccgtc cgaggtaaat ggcaccctgt acatgggccc cgtggccatt actgagaaca
    17401 ctaccctgaa ggccagagct tacatggtga actatccacc ctcagggatc aggaccgccg
    17461 tgtataacct ttttgccagg cccgtgagct ttgatcctcc cggggggacc tataacgcca
    17521 accagaatgt gaccttgagc accgtaactc ctggggctac catctggtac accaccgatg
    17581 gcagcgatcc cattccaggt gtgagcagcg tgtttgatcc tctcaatcct gtcctggtgg
    17641 atcacaacct gaccatcaaa gcgattgccg gcctgaacaa ctggttctcc tctcccatca
    17701 cctctgccag ctacatcatc gatatcccgc tgcccaacat cgcggtagtg cagattctgc
    17761 ctcgcagcgg tgtctatacc gccccggtgg atgtcagnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnntcc cattacgctg acttctacaa ccaacgtgaa agccgtggcc
    17941 tacaagaacg ggatgaacag atcgctcatc tccaatgcca gctatgtgat caatatcatc
    18001 attcccaacg tggaagcgcc ggtgttctca cttgtgtccg gactttatta cgctcccatc
    18061 gatgtggcga tcacgactgg caccgcggac gccgagatct ggtatagctt cgatggcagc
    18121 gatcccactc cagaggccgc tggttcgatg cgttactccg gagccatcaa tatccccggc
    18181 aacagcaccc gcttcatcaa agccagagct tacaagaccg gctggaacac cagcccggtg
    18241 gtctcagcca attataacgt gaccgggacg gtggcggaca tccaatacag cgttctctcc
    18301 ggcacttaca ccactcctca gagcctggtg ctcacaactt ccactcagga tgccaccatc
    18361 cgctacacca ccgatggcag tgatcccacc gccacctcga ctccatacat caccgccatc
    18421 ccgattaatc tctcaatgac cgtgaaagcc agaggttaca agaccaactg gttgccggga
    18481 aatccaggga tggaagtata tgtgatcacc ggaagtctgg cctttgatca gcccagttta
    18541 agcccggccc ccggaacttt tggcaatgcc atcagcgtca ccattgcaaa tcccatcccc
    18601 ccagacgcca atgtctatta cactacggac ggcacggtgc cttcagctac caacggcaca
    18661 ctctacagcg gtcctatcgc ggtgaacgcg gccctcacct tgaaagcggt ggccctgaaa
    18721 gcaggctggg tgcctgccta tctggatggg acctacggct tccaggccgc gaccccgggt
    18781 tttctgcctg gttctggtgc ttatcccaat gcccagctcg taagcctcag cacctcaacc
    18841 gacggtgcgt ccatccgcta taccactgac ggcaccgatc ccagcgctct gaacggcagc
    18901 gtatattccg ctccgatcag tgtggaagta aatcagacca tcaaagccta tgcctttaag
    18961 cccggctatc tggattctcc aatcgtggct gctacctatg ccatcggaac ctatattccg
    19021 gtggtggaca ctccggtgct gaatcctgct tccggcactt ataccgaaac ccgaagtgtt
    19081 gcgatgtcag tcggcactcc aggagcggtg attcgctata ccacagatgg cagcgaaccc
    19141 accgaagcct cagacgaata cacagcgccg gtcagcctcg ccctcaacac cgtgaccacg
    19201 atcaaagcca aggctttcaa ggtggactgg atgcccagcg ctaccaccac ggaactctat
    19261 gtgattactg gtcaggtggc agctcccatg ttcattcctc cggccggcag ttacaccgcc
    19321 gctcagaatg tggtgttgac ctcagccacg gaaggagctt tcttccgcta taccctggat
    19381 ggctcggacc ctgtagctgg ctcacccctc tacaccactc ccatcaacgt tccgcaaaac
    19441 ctgatcacga ccatcaaggc ccgtgcctat aagagcggtt gggcagacag cgatgtgagc
    19501 accgcggtct ataacgtcac cggaaaggtt gccttcactc cgcccgcctt tacccctcct
    19561 tcaggaactt atgccaccgg gcagatgatc aacatttctg cacccgtgcc cgcctctgcc
    19621 acagtcaaat acaccataga cggcactttg cccagccgga ccaacgggat tgagtacacc
    19681 gctcccttca gcctggcaac aggcgcaacg gtgcaggcaa tcgcttatct ggacggatgg
    19741 gaagactccc aggtcgtatc agctcaatac aacttcaacg tggctgctcc cgtttacact
    19801 cctcaatccg gtacttaccc aagtctgcaa gtcaccatcg gtagtgccac tcctggatct
    19861 caaatccgtt acaccatgga cggcagtgat ccatccgata cagtagggac gctctatacc
    19921 ggtcccatcg atctggctgg caacaacagc taccagttca aggctatcgc ttatctggcc
    19981 ggctggcaga acagcacgat tgtcccagcc acatatctgg tgaccggcac cgtggcagat
    20041 gtggt
//
LOCUS       DAOSFN010000024        20035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2178_length_20035_cov_8.854655, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000024 DAOSFN010000000
VERSION     DAOSFN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 20035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20035
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2178_length_20035_cov_8.854655"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..941)
                     /locus_tag="PLO35_02905"
     CDS             complement(192..941)
                     /locus_tag="PLO35_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH78644.1"
                     /translation="MNKIEPIISVRGLEARYADRVILKDIDVDIWPGEVTVILGGSGC
                     GKTTLLKNILRLQEPYRGSVKFWGQEVLQMDEPSFNQVLRQVGMLFQNGALLNSISVL
                     DNVAIPLEQHTKLSRRVIERIIRVKLRLVGLSHAIHMLPSELSGGMKKRAGLARAMVM
                     DPRVLFCDEPSAGLDPLTSAALDDLILDLKQQLNMSIVMVTHELASIHRVADRVIFLE
                     AGAMLFHGTLTEAKQAGIPAIERFFEAGRFE"
     gene            complement(946..2028)
                     /locus_tag="PLO35_02910"
     CDS             complement(946..2028)
                     /locus_tag="PLO35_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HOH78645.1"
                     /translation="MYKLQLESSALALTGRLTRDDVPAFLKEARDILKGKQPRALDLS
                     GLEYLDSAGVAGLEELILELKARVPELKIIPSTPEVEQVRQTFQDSDQPVTKPVSPGP
                     FESLGESILQGLAGFKEAFVLCSELAWWSLAGLFDKKGQRKGSFTAQGVLLGYNALPV
                     VCLLSFIIGFILSLQSAAQLKIYGGNVFLPDLLAVTLVREMGPLITAIIVAGRSGSAI
                     ASEIATMQVSEEIDALRMMAINPLRYVVVPKFHAITVVMPLLVVFSIIVGELGGLIIG
                     MSMLDLSLTMFVSRSIEIIALKDILISLGKSIVFAWLIVVIGAYYGFRVKGGAEGVGK
                     ATTASVVAAIFAVVIADAIFSLFYLA"
     gene            complement(2028..4196)
                     /locus_tag="PLO35_02915"
     CDS             complement(2028..4196)
                     /locus_tag="PLO35_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOH78646.1"
                     /translation="MKFKLIIYIALLTGLALTLSGCLSKVDRLTTNYYILDYKKATEN
                     PILKLSEPFPKTCEVFDTEVNRTYSRNQLVVKENFSKVSYLPGDLWANRLTDAIPNLL
                     VQRLKAYNLFRQVERSTGDISPNYYLETNLLNIEKVMSDNPRAYLRMEFKLRDAASQR
                     VLVPYLHDSYKPLISAEPVHLVETYNEMLMEATDFFVAQCRLFMEGKPLLPVLSKSSE
                     DPLEQFVGTQSEIMNSQISDGELLVLLSSETDAEIHYSYLEIGEEVPIKRQGIIGVPE
                     TVRPGKYKLLLGEKEDYPVNVQVDPKRRTTVQGQWGELLVLIMDQSQSKVRLGYNLWR
                     KKPDAYDYYYYGSDTSLGDEDFGQKEKVWLLPPGTYMFKIGSGPWDDLKDFTTVTIAK
                     GDSKLLTVIVDPTGDRNYMLGAGLLGNKDAVLGKSRLHKGTLTLDFNITASNDISEDD
                     PSYDVSLVGRTDNVLDYQYRSMRFTARSLYSLGLRLVTNTDLRVNPDTYSLKNVVLYT
                     PLREVKFLRNFSFYGRADVNTHFFDEILHYQEPRNIILTCARGDTLWRAPEQDQVRTK
                     IALFPLRLKEGLGITYRWVLSPRFSASIRGGYGWQQDINKRSFSSLYTGVPSQVPGDT
                     LRYDVYTEACSNYDSGIESTLVVSAISLLNFLTINSSIDVLFPMTNDQEPRFDSETRF
                     NIKLYRNLSMDISLNVEYDKARKDWVVYNYTSFLRLAIFY"
     gene            complement(4371..5933)
                     /locus_tag="PLO35_02920"
     CDS             complement(4371..5933)
                     /locus_tag="PLO35_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOH78647.1"
                     /translation="MLSRAQKIRLGAFIMVGSLLLLMILIAIVGNRLVDKKDTYYISF
                     ENYSVSGLQVGGTVNYSGIKVGRVENIKIDPKDVTKVIITISVDRGTPIKVDTQATLA
                     YMGITGIKAVELTGGTNQAARLKPKSFIKTGSSMIDDISDRALSIADKIDIIAANIGS
                     LTDEENRRNIADILSQTSMLLNDTRENLSTTMISLNQVATNTARLTDGLNDSISRITD
                     TFVDNVNQLTQTSSGSISELSQSTQSNLTRLTDSATTGISDLSESTQSNLTRLTDSAT
                     TSISGVTDNVNRVMSRMSDDVSSKLDLLTRSATGSLDSISMATTGSLRALTLSTSSSL
                     NAITKSTTSNLDSLGYTTKASIERLVTQLSSELDLVSGSLDRSINEISASANQLLADT
                     RIQLNEVGSNSNQMILSTTRQIAELSAQINRSLLRVNGIMESPELASIITNLNVLSGQ
                     LSQANLKDLVVELTTTLNRASTAIATIDRTLLRNRANINETLESLREASVNLNEFSRQ
                     IAEQPSTIIRGN"
     gene            complement(5933..>6163)
                     /locus_tag="PLO35_02925"
     CDS             complement(5933..>6163)
                     /locus_tag="PLO35_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78648.1"
                     /translation="NPRNLRSNLRNLREIRELQIIKLPNGFVPTAFSTSTTASKLLPS
                     QASQNPRNLRSNLRNLREIRELLETRMIRGDG"
     assembly_gap    6164..6263
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6264..6569)
                     /locus_tag="PLO35_02930"
     CDS             complement(<6264..6569)
                     /locus_tag="PLO35_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78649.1"
                     /translation="MRAASFLSFPQITQIGTQISQIIKLSNGFVPTSFSTSTTASRLL
                     PSQASQNPRNLRTNLRNLREIRDSQIIKLPNGFVPTAFSISTTASRLLPSQASQNPRN
                     "
     gene            complement(6566..7936)
                     /locus_tag="PLO35_02935"
     CDS             complement(6566..7936)
                     /locus_tag="PLO35_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOH78650.1"
                     /translation="MSAHNQQFVKLMKAAGIADPVIRTFGAYYEKLVRGEKGFLPETA
                     IRPPSETNLVSYDQIASRRSLSMLKRTVVVKLNGGLGTSMGLSKAKSLLPVKGNMNFL
                     DVITRQVLALRASSGYDVQLLFMNSYNTDADTLNYLSKYPDLSRQDLPISFVQNKYPR
                     VRQDDLAPFEPSDKNQLWNPPGHGDLYTAMAANGLLDSLIDKGYRYAFVSNSDNLGAT
                     VDTAIPAWMEANQVNFLMEVCERGEVDRKGGHLCEDASGQLMLREIAQTPAEDLARFQ
                     DIAYYKYFNTNNLWIDLKALQWSLISSDGVFLLPLIINPKTVEGIPVYQLETAMGAAI
                     SVFNNTRALVVPRSRFAPVKKNTDLLAIWSDAFELNDQYQIVLRRGLSVPPVIDLDDA
                     NYGRIDQMLAKFKDGVPSLMDCKYFKLKGDISFGEDVICEGKVSIEASQPIHLKSRLL
                     TGEIKL"
     gene            complement(7933..9954)
                     /locus_tag="PLO35_02940"
     CDS             complement(7933..9954)
                     /locus_tag="PLO35_02940"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOH78651.1"
                     /translation="MPQKSVFKVELSAELPPAPMFEPGIRRAPDRGLDLGPQDVKQAL
                     KNALRYIPQHLHPILAPEFLHELTTMGRIYGYRYRPQGRIFGRPIDSYQGITPARALQ
                     VMIDNNLDFDIALYPYELVTYGETGQVCQNWMQYQLIMKYLQVMRADQTLVVQSGHPL
                     GLFPSSPQAPRVISTNGLVIGKWDNPVEFKRLTALGVANYGQMTAGGWMYIGPQGIVH
                     GTYITLLNAGRKYLGIPADQDLAGVLYISSGLGGMSGAQSKAVEIAGGIGIIAEVDPS
                     RIETRHSQGWVSKVSSDPAEIFGWVEEYRRSRKPVSIAYHGNIVDLLEYVDIHDIKAE
                     LISDQTSCHAVYEGGYTPAGITFEQGRELLARDPQSFHQKVDESLRRHYRVIKRLCAK
                     GARFWDYGNSFMASVFDAGEIQIARNGQDTNDGFIWPSYVEDIMGPMCFDYGYGPFRW
                     VCLSRKDEDLRKTDAAARALIDPLRNSMDRDNYHWISNAEENKLVVGTKARILYADEE
                     GRVRIARKFNQMVRDGEIGPVMLGRDHHDVSGTDSPFRETANIRDGSNVMAEMATHCF
                     GGNIARGMTLVVLSNGGGVGIGRSINGGNGIVLDGSPRMDEVVTSALSWDVMGGVARR
                     NWARNPGAIETVEAWNAKHALEGAITVPAPVDETVIDALIGDTRMGGKL"
     gene            complement(10573..11535)
                     /gene="dusB"
                     /locus_tag="PLO35_02945"
     CDS             complement(10573..11535)
                     /gene="dusB"
                     /locus_tag="PLO35_02945"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOH78652.1"
                     /translation="MTLADFSGQKIWLAPLAGYTDRAFRVLCKRHGADVLVSEMVSAD
                     GLTRESFKTSKLIHFGEEERPYAVQLFGSDPLVMARGAALCLEFQPDLIDINMGCPVK
                     KVIKRGAGSALMTDPPRAAAIVREVRNALEGRIPLSVKFRSGWDSGSLNYLEFGLLLQ
                     DSGVDLICLHPRTAKQMFSGTSNWAHITHLKSRLNIPLIGNGDIDSPEAALRMFEACG
                     CDGIMIGRGALGRPFIFNQIKELISQGIYHPSTRDQLRSIMSEHLELALQFKRETVVL
                     KEMRSQLCHYVKGVVGGAELRRQINTCPDATTLRALINNFPALK"
     gene            complement(11532..13262)
                     /locus_tag="PLO35_02950"
     CDS             complement(11532..13262)
                     /locus_tag="PLO35_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOH78653.1"
                     /translation="MHQLNENREINVMLNQVVASINSLAEQQVLHIQRLTKIGEALSS
                     VTDLDMIFDMILEEAIEFTNADGATIYKVNEESRQLEFEIVYNRTMKMRQGGTHGVVN
                     WPSIPLFDEDGKPRLSHIVTAVYHSKKCVCFDDVYETKDYDISGTIKTDQTYGYRCKS
                     MLTLPLKNHEDEVLGIIQMINATDTGGNVISFNDEHRTMLNSLASQAAIALSNRRLIE
                     SLETLLLQFMQSIAKGIERKSKYSSDHITRVARLTDMFALKLNEAPKGVFKDLHFSDS
                     ELKELSMAGMMHDVGKIITPEFIMDKSTKLETILDRIDLVQERFKNMKLLFRYLRHEL
                     SEDQFAAYVADTLGTQAAPGELESYLDAELAFIQKINIGGEFLPDGDIERIERLSAIK
                     FECEGEEYLLITAEEKRNLQIRKGTLMPEEIKVMSQHVFVTWEMLSELSFPKKYSNVA
                     FYAATHHEKLNGKGYPWGLTADKLPIQSRIIAVADIFEALTAADRPYKKAKTLSESLK
                     IMAFCVKDGDLDADLLDFFLDSGLYLDFANRFMNPDQIDSPDISALRKIYRPVGSDSD
                     PARATTSSSK"
     gene            complement(13278..13886)
                     /gene="trmB"
                     /locus_tag="PLO35_02955"
     CDS             complement(13278..13886)
                     /gene="trmB"
                     /locus_tag="PLO35_02955"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOH78654.1"
                     /translation="MLSDRDYFLLPQDLGLMDYRQVFGNDHPVYVEIGSGKGEFISQY
                     PILHPHSNFLGFEVRDKRVRNILKKLDPARHPNVRLVELLVDHRITESLPRESIHGIF
                     IQHPDPWPKKRHFKRRLIQPAFLDAMASILVPGAFVQISTDHVEYANWIAEEFLRNSF
                     YESVYDETIQSTSHLEDHVITWFEQEQRRLGYEPNFMLFKKI"
     gene            complement(13873..14583)
                     /locus_tag="PLO35_02960"
     CDS             complement(13873..14583)
                     /locus_tag="PLO35_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78655.1"
                     /translation="MTQSSFRPELLLLLLFPLLVTYLALGLFDNKEQAQTQTMDSHMQ
                     ATFGDSAYSLVRIAGGEDAPPVSKPQRKTIGNLEFSLALALIIGLAGLLGHYLRSRWF
                     WYVIITVALIFTVLGSSYVGIKLTAFFLPNLILAAVLALIITRLFYNGSLIRIRMVLC
                     SILGAIVITLYYRLLYIFTGQEFTAEDWQSRFVVALINLIFITFGMSLADLVITGLAR
                     KRVQPLPMAEDEDEDAER"
     gene            14773..15759
                     /gene="ftsY"
                     /locus_tag="PLO35_02965"
     CDS             14773..15759
                     /gene="ftsY"
                     /locus_tag="PLO35_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOH78656.1"
                     /translation="MLSIMRNLRQKLAKTKSGFIGRIAEAIRLRGKVDEELMEEIEDI
                     LLRCDTGVEMTQLIMERLTTRIRVDRIDDASEVQDALQEIMRDILLTIDDQDLEADSD
                     ERGFFEEVDAKPWVIVFVGVNGVGKTTSIGKIAYRFRKLGKSVLIVAADTFRAAAIEQ
                     LAIWAERAGAGFIKSQPDADPSAVIYDGINSAIARDIDVVLIDTAGRQHTKDKLMNEL
                     SKIDRTIKKLIPDAPHHALLVLDSTTGQNAISQASHFDKAMALTGLILSKYDGTSKGG
                     IIFNLKHNLKLPVRLIGIGEGIEDLEDFNISDFVAAFFSKEGSQESVNEEEQ"
     gene            15756..15992
                     /locus_tag="PLO35_02970"
     CDS             15756..15992
                     /locus_tag="PLO35_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78657.1"
                     /translation="MKNTALKFINPILFILVLTTFIAMLLSKFGPESMRGSEDLSVIH
                     TWAGTLFFIVGTIHLIYNWGWVRTNIIGKRKKHK"
     gene            15989..19237
                     /locus_tag="PLO35_02975"
     CDS             15989..19237
                     /locus_tag="PLO35_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOH78658.1"
                     /translation="MTLLHQDIASDILNRLLSICTGPDSTKARIILLRGLLDELYKEL
                     TRDASVSLSGLFARMQFLREQVSLPDALNQQANLLRILGNQCAHENGFEPSDTDLFSC
                     AWVMRALLHWYQEELDLNELDQFLQEHRASPFPDRPHARKHSFLGVVLSWKLKHGDQQ
                     NTGIEITAAEEDGRSVGIYLRDDPKSEGRKWSLLDRCLWKWATINCLNLSESSGAADQ
                     YIDNPGTMIVIEPDYLIDVSALAECVSSKGVNPELAVLGRLISEESTQSLAIGSAVNN
                     IFDELMFDPDADYNQLFRDSMARAPIPLVALGKEAALQVHGIIRDEHLPRLRAMARFA
                     HQRDMMLEPSFISPKYGLQGRLDLLYKDKGRYYIVELKSGNAPQTGIWPQHLYQVVGY
                     NLLIKECYGAANLGTSSILYSKLPDDKLRGVTNLISQEQELLMCRNRVIGIWKLLSEN
                     PATFFDWLKSHEGNQLAGFVKERLHRVQSILNALDPPEYEWYLEQVRLAVRETWYVKI
                     GSGTHRSESGYGYNALWQQSREEKLRRYKLLPALKLVAIDKNLLRFKLEDRDRISDFR
                     EGDVVVAYREDRSVSAQEIIRGELITLGEETLLVRARGVLNPTLFADLDRLWSIEHDL
                     LESMLFGTLASITGFLAAPENKRRKLLGLMAPEFQTDGVASAEDVESIVAQIDAARDY
                     CVVQGPPGTGKTSGLITSFIRRLYQNSDQTILILSFTNRAVDEICSNLARHQIPHIRT
                     GRSQNVELELLGNLIRDKRFDEIEEIVKTNRVWVATVQSCNSWINDWLRIIPGIGTLI
                     IDEASQIIEPNILGIIARAERCILIGDQNQLPPIVAQTDATYSFTAAELRQLCYSGYN
                     RSLMERLFLLSEQNGWPQARFMLNRHYRMHDEIAALVQQFYHHDLISCTPRQQRTLES
                     SETTPAYLKKRVVWIDCPPATHAYYDEEQCVIIHRLLDTLIQLGEITELATGVGIVAP
                     YRAMIQALRAELGSPFSAMTIDTVERFQGSERDIIIITLPLRDKADLRTLEAVSDDQK
                     VDRKLNVSLSRAKERLIILGNSSLCASSGHYAFLIDRIRASQTLIPSSQLID"
     gene            19264..19572
                     /locus_tag="PLO35_02980"
     CDS             19264..19572
                     /locus_tag="PLO35_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOH78659.1"
                     /translation="MAQPNQPNQINIKIDEKVGEGTYANFFVITNSPSEFIMDFGRIL
                     PGIPDAKIYTRVVSTPSHAKQFLQLLEKNIETFEKQFGEIKVQGMPENKPVGFKPDRG
                     "
     gene            19671..>20035
                     /locus_tag="PLO35_02985"
     CDS             19671..>20035
                     /locus_tag="PLO35_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOH78660.1"
                     /translation="MKKFSWRGLTILIFCLGTAFFLAPLAFDGLPSWWASQKLNLGLD
                     LKGGMQIILEVDTSKLPANQVAGAVDNNIEIIRNRIDQFGVAEPIIQKMGEKRIVIQL
                     PGVKDHLAAESLVKKTAML"
BASE COUNT         4668 a         5314 c         5305 g         4648 t
ORIGIN      
        1 atctgcggga aatatgtaat ccgtgagaaa caggttgaga gtttcacgca gattatgctg
       61 attttttgag ggtttagacc catgcctgtt caacatcatg agcctcctca accatctcta
      121 tagtatctgc gagatctgcg tataaatctg cgtaatctgc gggaaatatg taatccgtga
      181 gaaaagggac atcactcaaa cctgccagcc tcaaaaaacc gctcgatagc tggaatgcca
      241 gcttgtttgg cctctgtcag ggtcccgtga aacaacatcg cgcccgcctc caaaaagatc
      301 acccggtccg ccacccgatg aatcgaggcc agctcgtggg tcaccatcac gatgctcatg
      361 ttgagttgct gcttgaggtc caggatcaga tcatccagag cggccgatgt gagggggtcc
      421 aggccagctg aaggttcgtc gcaaaagagc acgcgggggt ccatcaccat cgctctggcc
      481 agaccggccc gtttcttcat ccctcccgat agttccgacg gcagcatgtg gatggcgtgc
      541 gacaggccta cgaggcgcag tttcactctg atgatccttt cgatcactct gcggctgagc
      601 ttggtgtgct gttccagcgg gatagccacg ttatcgagga cagaaatgga gttgagcagc
      661 gcgccgtttt gaaagagcat gcccacttgc ctgagcacct ggttgaagct gggttcgtcc
      721 atttgtaaga cttcctgtcc ccaaaacttc acactgcccc gatagggttc ctgcagcctc
      781 aggatgtttt tgagcagcgt ggtcttgcca caaccgctgc ctcccaggat cactgtcact
      841 tcacccggcc agatatccac atcgatatct ttcaggatca cccggtcagc gtatctggct
      901 tccaggcccc gcacactgat gataggttcg atcttgttca taagactatg ccaggtaaaa
      961 caggctgaag atggcatccg cgatcacgac cgcgaagatt gccgctacca cagaagctgt
     1021 ggtggcttta ccgacaccct ctgctccacc tttgacccga aaaccgtaat aagcgccgat
     1081 caccacgatc agccaggcga agacgatgct cttgcccaga gagatcagga tatctttgag
     1141 tgcgatgatc tcgatactgc ggctgacaaa catcgtcaag ctcagatcca gcatgctcat
     1201 cccgatgatc agacctccca gctctcccac tatgatggaa aacaccacca gaagcggcat
     1261 cacgaccgtg atagcgtgaa acttggggac caccacatag cggagcggat tgatcgccat
     1321 catccggagg gcgtcgatct cctcagagac ctgcatagtg gcgatttctg aggcaatggc
     1381 ggaaccactg cgcccggcca cgataatggc ggtgatcaaa ggccccatct ctctcaccag
     1441 cgtgacggcc agcagatcag gtaaaaacac attaccgccg taaatcttta gctgcgcggc
     1501 agactgcagt gacaggatga agccgatgat aaaagacagc agacagacca ccggcagagc
     1561 gttgtagccc aaaagcactc cctgggccgt gaaactgcct ttgcgctggc cttttttatc
     1621 gaacaagccg gctaaagacc accaggccag ttcactgcag agcacaaacg cctctttaaa
     1681 gcccgccagg ccttgcagga tagattctcc cagactttca aagggaccgg gcgaaactgg
     1741 tttggtaact ggctgatcgc tgtcttgaaa tgtctgcctc acttgctcaa cttcaggggt
     1801 tgagggaatt atcttcagct ccggtaccct tgctttgagc tccaggatca gctcctccag
     1861 tccagccacg ccagcgctgt ccagatattc cagcccgctg aggtctaaag cgcgcggctg
     1921 cttgcctttg aggatgtctc tggcttcttt cagaaaagcc ggcacatcgt ccctggtaag
     1981 cctgccagtg agtgccagcg cggatgattc gagctgcagt ttatacatca atagaaaatc
     2041 gccagcctga gaaaggaagt gtagttgtag actacccagt ctttgcgggc tttatcgtat
     2101 tccacattca agctgatatc catggagaga ttgcggtaaa gcttgatatt gaagcgggtt
     2161 tcactgtcaa aacggggttc ctggtcgttg gtcatgggaa acagcacatc gatgctggag
     2221 ttgatggtga gaaagttcag gaggctgatg gccgatacca ccaaagtgct ttctatcccg
     2281 gaatcatagt tggaacaggc ctctgtatat acatcatagc gcagagtatc tccaggcacc
     2341 tgacttggca cacctgtata aagcgaagaa aaggagcgtt tgttgatatc ctgctgccat
     2401 ccatagccgc ccctgatgct ggctgagaat ctgggactca gcacccagcg ataggtgatc
     2461 cccagtccct ctttcagcct gagcgggaac agggcaatct tggtgcgcac ctggtcctgc
     2521 tctggcgccc gccagagtgt gtctcccctg gcgcaggtca gaatgatgtt tctgggctcc
     2581 tgatagtgaa gaatctcgtc aaagaaatgg gtgttcacat ccgccctgcc gtagaatgag
     2641 aagttgcgga ggaattttac ttcccgcaga ggagtgtaga gcaccacatt cttgagagaa
     2701 taggtgtctg gattgacgcg caaatcggta ttggtgacga gcctcaatcc caggctgtaa
     2761 aggctgcggg cggtgaaacg catagaccgg tattggtagt ccagaacatt gtcggttcgt
     2821 cccaccagag acacatcgta gctgggatcg tcctctgaga tgtcgttgct ggcggtgata
     2881 ttgaaatcca gggtaagcgt ccccttatgg agcctgcttt tacctaatac ggcatctttg
     2941 ttgcccagca agcccgcacc cagcatatag ttacggtctc cagtgggatc cacgatcacg
     3001 gtgagcagtt tggaatcccc cttggcgatc gtaacggtgg taaaatcctt cagatcgtcc
     3061 cagggaccgc tgccgatctt gaacatatag gttcccgggg gaagcagcca taccttctct
     3121 ttctgtccaa agtcctcatc tccaaggctg gtgtccgatc catagtagta ataatcatac
     3181 gcgtccggct tttttctcca caggttgtag cccagcctca ctttgctctg cgactggtcc
     3241 atgatcagca ccagcaattc accccactgg ccctggacgg tggtgcggcg tttgggatcc
     3301 acctgaacgt tgacagggta atcttccttt tctcccagaa gcagcttata cttcccgggc
     3361 ctgacggttt ctggcacgcc aatgatccct tgccttttta tcgggacttc ctcacctatc
     3421 tcaaggtagc tgtaatgaat ttccgcgtcg gtctcagagc tgagcagcac cagcaactcg
     3481 ccgtcggaga tctgtgaatt cataatctca gactgagttc ccacaaactg ctccagcggg
     3541 tcttccgagg atttggacaa aaccggcagc agtggtttgc cctccataaa gagacggcac
     3601 tgggccacaa aaaagtccgt ggcttccatc agcatctcgt tgtaggtttc caccagatgc
     3661 acgggttccg cggagatcaa aggcttgtag gaatcatgca gatagggaac cagcactctt
     3721 tgcgaggcgg cgtcccgcag cttgaattcc atcctgagat aagccctggg attatctgac
     3781 atcacctttt cgatgttgag cagattggtt tccagatagt agttggggga aatatcaccc
     3841 gtggaacgct ctacctggcg aaagaggttg taggccttca gacgctgcac gaggagattg
     3901 ggaatggcgt ccgtcagacg gtttgcccac aggtctccgg gcagatagga aaccttggaa
     3961 aagttttctt tgaccaccag ttggttgcgg ctgtaggtgc ggttcacttc cgtgtcgaag
     4021 acctcacagg tctttggaaa gggctcactg agtttcaaga tggggttttc ggtggctttt
     4081 ttataatcca ggatatagta attggtggtc agtctgtcca cctttgacaa acatccgctc
     4141 agcgtcaaag ccaggcccgt cagcaacgcg atgtagatga tgagtttaaa cttcatgaca
     4201 aacctcactt ttttaaaaca gaggaacaga gaattgagaa ttgacaattg acaattgaac
     4261 agctctcgcc attggagccg taaaagtcct cgtttgcgga ggccagtaac gtcgaatgcc
     4321 aattcggtgc gtgatcataa cgcataccgc gtaacgcgta acgcatatca tcagttcccc
     4381 cgaatgatgg tggagggttg ttccgcgatc tggcgcgaga attcattcag attgacactg
     4441 gcctctctga gggattcaag ggtctcattg atgttggcgc gattcctcag gagagtgcga
     4501 tctatcgtgg caatcgcggt ggaggcacga ttcagcgtgg tcgtgagttc cacaaccaga
     4561 tccttgagat tggcctgaga gagttgcccc gagagcacgt tgaggtttgt gattatgctg
     4621 gccagctcgg gagattccat aatgccgttc actctcaaga gggagcggtt aatctgagcg
     4681 ctcagctctg cgatctggcg cgtggtgctc aggatcatct ggttgctgtt ggagcccact
     4741 tcgttgagct gaatcctggt atcggccagc agctgattgg cgctggcgct gatctcattg
     4801 atgctcctgt ccagactgcc gctcaccaga tcaagctcgc tgctgagctg cgtgaccagt
     4861 ctttcgatgc tggccttggt ggtgtagccc aggctgtcaa gattggaggt ggtggatttg
     4921 gtgatcgcgt tcaggctgct ggaagtggag agtgtcaggg cacgtaaact tccagtagtg
     4981 gccatagaga tcgaatcgag cgatcccgtg gcggagcggg tcaaaaggtc cagtttgctt
     5041 gatacatcgt cactcattct gctcataacc cggttgacgt tgtctgtcac cccggagata
     5101 ctggtggtgg cgctgtctgt gaggcgggtc agattgctct gagtggattc tgacagatct
     5161 gagatcccgg tggtggcgct gtccgtgaga cgggtcagat tgctctgggt ggattgtgaa
     5221 agctctgaaa tgctgcctga actggtctga gtcagctgat tgacattatc cacgaaagtg
     5281 tctgtgatac gtgagattga gtcgttgagc ccgtcggtca ggcgggcagt atttgtggcc
     5341 acctggttca aagaaatcat tgtggtactc aggttttccc gggtgtcgtt gagcagcatg
     5401 ctggtctgag acaggatatc cgcgatgttg cggcggtttt cctcatcggt gaggctgccg
     5461 atattagcgg cgatgatatc aatcttatcc gcaatcgaga gcgcccggtc actgatgtca
     5521 tcgatcatgc tggagccggt tttgataaag cttttcggtt tcaaccgcgc cgcctggttg
     5581 gtcccccccg ttagctcaac cgccttgatc ccagtaatgc ccatatatgc cagcgtggct
     5641 tgggtatcaa ccttgatggg ggttccgcgg tccacactga tcgtgatgat caccttcgtc
     5701 acgtccttgg gatcgatctt gatattctcc acccggccaa ctttaatgcc actgtagttg
     5761 acggtcccgc ctacttgcag gccgctcacc gagtagttct caaaactgat gtaataagtg
     5821 tccttcttat ccaccagacg gtttcccaca atggcgatca ggatcatcaa caggagcagg
     5881 gaacctacca taataaaggc cccaagcctg attttttgcg cgcgtgaaag cattatccat
     5941 cacctcgtat cattcgtgtt tccagcaatt ctcttatttc ccgcagatta cgcagattgc
     6001 tgcgcagatt acgcggattt tgtgaagctt gagaaggcag gagcttggat gcggtcgtgg
     6061 aggttgagaa ggcagttgga acaaatccat taggcagttt aataatctgc aattctctta
     6121 tttcccgcag attacgcaga ttgctgcgca gattacgcgg attnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnattacgc ggattttgtg aagcttgaga aggcaggagc
     6301 ctggatgcgg tcgtggagat tgagaaggca gttggaacaa atccattagg cagtttaata
     6361 atctgcgaat ctcttatttc ccgcagatta cgcagattgg tgcgcagatt acgcggattt
     6421 tgtgaagctt gagaaggcag gagcctggat gcggtcgtgg aggttgagaa ggaagttgga
     6481 acaaatccat tagacagttt aataatctgc gaaatctgcg taccaatctg cgtaatctgc
     6541 gggaaactaa ggaaacttgc agctctcata acttgatctc acccgtcagc agacggctct
     6601 tgaggtgaat gggctgtgag gcctcgatgc tcacctttcc ttcgcagatc acgtcttcgc
     6661 caaaactgat atcacccttg agcttgaaat acttgcaatc catcagggac ggcactccgt
     6721 ccttgaattt tgccagcatc tgatctatcc tgccatagtt ggcatcatca agatcgatca
     6781 cgggcggaac gctgagccca cgcctcaaca cgatctgata ctggtcgttg agctcaaaag
     6841 catcgctcca gatagccagc agatcggtgt tcttttttac aggcgcgaag cgggaacgcg
     6901 gcaccaccaa agcccgggtg ttgttgaaca cactgatcgc ggcccccatg gcggtttcga
     6961 gttggtagac cggtatgcct tccaccgtct tgggattgat gatcagaggc aacagaaata
     7021 ctccgtcgga agagatcagg ctccattgca gggctttgag atctatccac aggttgttgg
     7081 tattaaaata cttatagtaa gcgatatcct gaaacctggc caaatcctcc gcgggtgtct
     7141 gagctatttc ccgcagcatc aactggccgc tggcatcctc acaaagatgg ccacccttgc
     7201 ggtccacttc accccgctcg cagacttcca tcaggaaatt gacctggttg gcctccatcc
     7261 aggccggaat ggctgtgtcg acggtagcgc ccaggttgtc tgagttggag acgaaggcgt
     7321 agcggtaacc cttgtctatc aggctgtcca gcagtccgtt ggcagccatg gcggtgtata
     7381 ggtcaccatg tccgggggga ttccacagct gatttttatc cgagggctcg aagggagcca
     7441 gatcgtcctg acgtactctg ggatacttgt tctggacaaa ggatattggc aagtcctgac
     7501 ggctgagatc gggatacttg ctcaggtagt tcaaggtgtc ggcgtcggta ttatagctgt
     7561 tcataaagag gagttgcacg tcgtatccgg agctggctct cagggccagc acctgacgcg
     7621 tgatcacatc caggaaattc atattgccct tcacaggcag cagagatttg gccttgctca
     7681 gccccatcga agttcccagg ccaccgttca gcttgacgac taccgtccgt tttagcatcg
     7741 agagactgcg ccgcgaggca atctgatcat aggagaccag gttggtttca gagggaggcc
     7801 ggatggccgt ttcaggcagg aagccttttt cacccctgac cagcttctca taataggccc
     7861 caaaggtgcg gatcaccgga tcggcaatcc ctgccgcttt catcagtttg acaaactgct
     7921 gattgtgagc gctcatagtt ttcctcccat acgcgtgtct ccgatcaaag catcaataac
     7981 cgtctcatcc accggggccg gtaccgtgat cgctccttcc aatgcgtgtt tggcgttcca
     8041 ggcttccacg gtttcgattg cgccggggtt gcgggcccag tttctgcgcg ccacacctcc
     8101 catcacgtcc cagctcagag cgctggtcac cacttcatcc atcctggggc tgccatccag
     8161 cacaatgccg ttgccaccgt ttatggaacg tccgatgccc actcctcccc cattggacag
     8221 caccaccaaa gtcatgcctc tggcgatgtt acctccaaag cagtgagtgg ccatctcggc
     8281 catcacattg ctgccgtccc ggatattggc ggtttcacga aaaggtgaat ccgtgccgga
     8341 gacgtcgtga tgatcgcgac ccagcatcac aggtccgatc tcgccgtcgc gcaccatctg
     8401 gttaaacttg cgggcgatcc tcaccctgcc ttcttcatcg gcatagagga tgcgggcttt
     8461 ggtgcccacc accagtttgt tttcttcggc gttagatatc cagtgatagt tatcgcggtc
     8521 catggagttg cggaggggat ctattaaagc ccgggccgcg gcatcggtct tgcgcaggtc
     8581 ctcatccttg cggctcagac agacccagcg aaaaggacca tagccataat caaaacacat
     8641 cggccccatg atgtcttcca cataggaagg ccagataaaa ccatcgttgg tgtcctgccc
     8701 gttgcgtgcg atctgaatct ctccggcatc gaacaccgag gccataaagc tgttgccata
     8761 atcccagaaa cgtgctccct tggcgcagag ccgtttgata acccgatagt gacgacgcag
     8821 cgattcatcc actttttgat gaaagctctg cgggtcacgg gccaagagct cccgtccctg
     8881 ctcaaatgtg attccggcgg gggtgtagcc cccttcataa acggcatggc aggaggtctg
     8941 gtctgaaatc agttcggctt tgatgtcgtg gatatccaca tattccagca aatccacaat
     9001 gtttccgtgg taagctatgg acacaggctt ccgggagcgg cgatactcct ccacccagcc
     9061 aaaaatctcc gccggatcgg aagaaacctt gctcacccaa ccctgcgaat gacgcgtctc
     9121 gatgcggctg ggatccactt ctgctatgat gccaattccc cccgcgattt ccaccgcctt
     9181 ggattgagct ccgctcatgc cacccagacc gctggagatg tagagcactc cggccaggtc
     9241 ctggtccgcg gggatgccca gatacttgcg gcccgcgttc aggagggtga tataggtgcc
     9301 gtgcacgatg ccctgaggcc caatatacat ccagccgcct gctgtcatct gtccgtaatt
     9361 ggccaccccg agggccgtga gccttttaaa ttccacggga ttatcccatt tgccaatcac
     9421 cagcccgttt gtggagatca ctctgggggc ctggggtgag gagggaaaga gccccagggg
     9481 atgaccgctc tgcaccacca acgtctggtc cgcgcgcatc acctgcaggt atttcatgat
     9541 gagctgatac tgcatccagt tctggcagac ctggccggtc tcgccataag tcacgagctc
     9601 ataaggataa agggcgatat caaaatccag attgttgtcg atcatcacct gcagggcgcg
     9661 ggccggagtg ataccctgat atgagtcgat aggcctgcca aagatgcgtc cctgcggacg
     9721 gtatcggtag ccatagatcc tgcccatggt ggtcagttca tgcagaaatt ccggcgccag
     9781 aatgggatgc aagtgctggg gtatatagcg gagagcgttt tttagggcct gtttaacatc
     9841 ctgcggaccg agatcaagtc cccggtcagg agccctgcga atgcctggtt cgaacatggg
     9901 agcgggtggc agttcagcgc ttaattcaac tttgaaaaca cttttctgcg gcatcattca
     9961 gaatcctctt tatgtaatta tattctaatt attgctgtag aaccgattac ccactttata
    10021 aacagaaatc actaagatac aatcggtttg gcatacaatg tctgatgtga taatatctgt
    10081 caagtcaaaa gtctggccag aacatcctga tcaaagtgta tccctgctat catactgtac
    10141 ctcagagggt ttcaccagga tttaatccca ctgggacgtt ctgcaatgca tcagaccggc
    10201 attggatact atcaccgctc aattcgtttc tttccgcgtc agttcttgcc tatttacccg
    10261 ccgttgagat accgtgcgct ctcccccgtt tttcgccgga ggccgcaccg tatctccacg
    10321 gcaactgatc aggcaggaaa aggacaagac cgttgatggg atggcgtggt tggctggggc
    10381 ggatggatga gaaagcgaga aattgatgaa ttgatggaat ggatgtgtga gattgacaaa
    10441 ctccttttat tccagaggga tagagatgaa tgagattgaa tccgcgctga agtagggaaa
    10501 ggtaacaagg cgctagggag aacctatttg attcgattgg agagcgtaga gatggtgaag
    10561 agcggtaatc agttatttca gggcgggaaa gttgtttatc agagctctga gagtggtggc
    10621 gtccggacag gtgttgatct gtctgcgcag ttccgctcct cccactacgc ccttcacata
    10681 gtgacatagc tggctgcgca tttcttttaa caccacggtt tcccgcttga actggagggc
    10741 cagctcaagg tgttcggaca tgatgctcct gagctggtca cgcgtgctgg gatgatagat
    10801 tccctgtgaa atcaattcct tgatctgatt aaagataaat ggtcttccca aagccccgcg
    10861 accgatcatg atcccgtcac aaccacaagc ttcaaacatt cttaaagccg cctcaggaga
    10921 gtctatatcg ccattgccga tcagcggaat gttaagccgg gattttagat gggtgatgtg
    10981 agcccagttg cttgtgcctg aaaacatctg tttagcggta cggggatgca gacagatcag
    11041 gtccacacca ctgtcctgca gcaggagccc aaactccaga tagttgaggc tgccactgtc
    11101 ccaaccgctg cgaaatttga cgctgagagg aatccggccc tccagagcgt tcctgacttc
    11161 ccttacgatg gcggcggccc tggggggatc tgtcataagc gcgcttccag ccccgcgttt
    11221 gatcactttt tttacagggc agcccatgtt gatgtcaatc agatcaggct ggaattcaag
    11281 gcataatgct gcccctctgg ccatcacaag cggatcgctt ccaaagagtt ggacggcata
    11341 ggggcgctct tcttccccaa agtggatgag ctttgaagtc ttgaaggatt ctcgggtgag
    11401 gccgtccgcg ctcaccattt cgctcaccag cacatccgcc ccgtgtcttt tgcagaggac
    11461 tctgaaggcc cggtcggtat atccggccag gggagcgagc cagattttct ggccgctgaa
    11521 atcagcgagg gtcattttga cgatgaggtg gtggcacgcg ccggatcgga atccgaccct
    11581 acaggcctgt agatctttct gagggcagaa atatcggggc tgtcgatctg gtcaggattc
    11641 ataaagcggt tggcaaaatc cagatagaga ccggaatcga ggaagaaatc cagcaggtcg
    11701 gcatcgagat ccccgtcttt gacgcagaaa gccatgatct tgagggattc cgagagggtt
    11761 ttggctttct tatatggacg gtccgcggcc gtgagggcct cgaagatatc ggccacggcg
    11821 ataatcctgg attggatggg cagcttgtct gcagtgaggc cccatgggta acctttgccg
    11881 ttgagctttt catggtgggt ggcggcatag aaggccacgt tggagtattt tttgggaaaa
    11941 ctgagctcgg aaagcatttc ccaggtgaca aatacgtgtt gggacatcac cttgatctct
    12001 tccggcatca gagtgccctt tcggatctga agattgcgct tttcttccgc ggtgatcagc
    12061 agatattctt caccttcaca ctcaaacttg atcgcggaga ggcgttcgat gcgttcgata
    12121 tctccatcgg ggaggaattc accgccgatg ttgatctttt gaataaaagc cagctcagcg
    12181 tctaaatagc tttccagttc tcctggagct gcctgggtac ccagtgtatc tgccacatag
    12241 gccgcgaatt gatcttcaga gagttcatgg cgcagatatc tgaagagcag cttcatgttt
    12301 ttgaagcgct cttgcaccag atcgatccgg tccaggatcg tctccagctt ggtgctcttg
    12361 tccatgatga attccggggt aatgatcttg cctacgtcgt gcatcatgcc ggccatggag
    12421 agctccttga gctcggaatc gctgaagtgg aggtctttga agacgccctt cggggcttca
    12481 ttgagcttga gggcaaacat gtccgtgagc ctggccaccc gggtgatatg atcagaagag
    12541 tatttgcttt tacgctcgat acccttggcg atcgactgca tgaattgcag taacagggtt
    12601 tccaaagatt cgatgaggcg ccggttggaa agcgctatcg cagcctgcga agccagcgaa
    12661 ttgagcatgg tgcggtgctc gtcgttaaag ctgatcacgt taccgcccgt gtccgtggcg
    12721 ttgatcatct gaatgatccc cagcacctcg tcctcgtggt ttttgagggg cagggtcagc
    12781 atcgatttgc agcgatatcc ataggtctgg tccgtcttga tcgttccgct gatgtcgtag
    12841 tctttggttt cataaacatc atcaaagcag acgcactttt tgctgtgata gactgccgtc
    12901 actatatgtg aaaggcgggg tttgccatcc tcgtcaaaga gagggatact gggccagttg
    12961 actactccgt gagtgccgcc ctgccgcatc ttcatggtgc ggttgtagac gatctcaaac
    13021 tccagttgac ggctctcttc atttactttg tagatggtcg cgccgtcggc attggtaaac
    13081 tcgatggcct cttccagaat catgtcgaag atcatatcca ggtccgttac cgaagacagc
    13141 gcttcgccaa tcttggtaag tctctgtata tgaagcacct gctgctcagc caaagagttg
    13201 atcgaggcta ccacctgatt gagcatgaca ttgatttctc tgttttcatt gagctgatgc
    13261 actattgaga cctcccgtca tatcttcttg aaaagcataa agttaggttc ataacccagg
    13321 cggcgctgct cctgttcaaa ccaggtgatg acatggtcct ccaggtgtga ggtgctttgg
    13381 atggtctcgt catagaccga ttcataaaac gaatttctca aaaattcctc cgcaatccaa
    13441 ttggcatatt ccacatgatc agtggaaatc tgcacaaaag ctcccgggac caatatgctg
    13501 gccatcgcgt ccaaaaaagc cggctggatg agacggcgtt tgaaatggcg ctttttgggc
    13561 caggggtcag gatgctggat aaagatgccg tgaatggact cccggggcag gctttccgtg
    13621 atgcggtggt ccactaaaag ctccaccagc cggacgttgg gatgcctggc ggggtccagt
    13681 tttttgagga tattgcggac gcgcttgtcc cgcacttcaa accccagaaa gttggaatgg
    13741 ggatgcagaa tgggatactg agagatgaat tcgcccttgc cgctgccgat ttccacatag
    13801 acgggatggt cgtttccaaa cacttgacgg tagtccatca aaccaagatc ctgaggcagg
    13861 aggaaatagt ctctatcgct cagcatcttc gtcttcgtcc tcagccatag gcaaaggctg
    13921 aacgcgcttt ctggccagac ctgtaatcac caggtccgcc agcgacatgc caaaggtgat
    13981 gaatatcagg ttgatgaggg caaccacaaa acgggactgc cagtcttccg cagtaaactc
    14041 ctggccggta aagatataca gaagccggta atagagggtg atcactatgg cgcccagaat
    14101 tgagcagagc accatacgga tgcgaatcag agagccgttg tagaagagcc tggtgataat
    14161 cagggccagg accgcggcca ggatcagatt gggtaaaaag aaagccgtga gcttgatccc
    14221 cacataggac gagcccaata ccgtaaagat cagggccacc gtgatgatca cgtaccagaa
    14281 ccagcggctc ctgagataat gtcccagcag accagccagt ccaatgatca gggccagcgc
    14341 gagactgaat tccagattgc caatggtttt gcgttggggt ttggagaccg gtggcgcgtc
    14401 ttcaccaccc gcgattctca ccagcgaata agcgctgtct ccaaaggtgg cttgcatgtg
    14461 tgaatccatg gtctgagtct gggcttgctc cttgttgtca aacaagccca gagccaaata
    14521 tgtgaccaat agcgggaaca acagcaacag cagcagttcc ggcctgaacg aactttgtgt
    14581 catctaatcc tcgggggtta tgggttttat ggttgaatcg ttttgggtta tcaggtttgg
    14641 acgatcgctc actcacgtga agcatcccgc ccgcaaagca tctaagcttt agattttcgt
    14701 tgactctgaa tgatggttca attttatggc aagctgttag aatgtcaaca acaatcgccc
    14761 aaggagagcc gaatgctgag catcatgaga aacctgcgcc aaaaactggc gaagacaaaa
    14821 agcggattca ttggcagaat cgctgaggct atccgcctca gaggcaaagt cgatgaagaa
    14881 ttgatggaag agatcgaaga catcctgctg cgctgcgaca caggcgtgga aatgacccag
    14941 ctgatcatgg aaagactcac caccaggatc agagtggaca ggatcgatga cgcctctgag
    15001 gtccaggacg ccttgcagga gatcatgaga gacattttgc tcacgattga tgatcaggac
    15061 ctcgaagcgg actctgatga gcgcggcttt tttgaagagg tggatgccaa gccctgggtg
    15121 atcgtcttcg tgggcgtcaa cggagtcggc aaaactacca gcatcggcaa gatcgcttat
    15181 cgcttcagga aactgggcaa gagcgtgctg attgtggcag cggacacctt cagggccgca
    15241 gccatcgaac aactggccat ttgggctgag cgggccggag cgggattcat caaatcccag
    15301 cccgacgccg atccgtccgc ggtgatctat gacggcatca actccgccat tgccagagac
    15361 atcgatgtag tactgatcga cacggccgga cgtcagcaca caaaagacaa gctcatgaac
    15421 gagctttcca agatcgaccg caccatcaag aaactgattc ccgacgcccc ccatcatgct
    15481 ttgctggtat tggattccac caccggtcag aacgccatct cccaggccag ccactttgat
    15541 aaagctatgg ccctcaccgg tttgatcctg tccaaatacg atggcacttc caagggcggg
    15601 atcatcttta acctcaagca caacctgaaa ctgccggtgc ggctgatcgg gatcggagag
    15661 ggaatcgaag acctggaaga ctttaacatc tcagattttg tggcggcatt ctttagcaaa
    15721 gagggttctc aagaatctgt caatgaggag gaacaatgaa aaacaccgct ctcaaattca
    15781 tcaaccccat cctgtttata ctggtgctca ccacctttat cgccatgctg ctctccaagt
    15841 tcggtcccga atccatgcgg ggcagcgaag atctgagcgt gatccacacc tgggcgggaa
    15901 cgctcttctt tatcgtcgga accatccatc tgatctacaa ctggggctgg gtgaggacca
    15961 atatcatcgg caagcgaaag aagcataaat gacacttttg catcaggata tcgcctctga
    16021 tatcctgaac cggctgctga gcatctgcac gggaccggat agcaccaaag ccagaataat
    16081 cctgttgcgc ggcctgctgg acgagctcta caaagagctc accagagacg cctctgtctc
    16141 gttgagcggg ctttttgcca ggatgcagtt tctgcgtgaa caggtctccc tgccggacgc
    16201 cctgaaccaa caggccaacc tgttacgcat cctgggcaac caatgcgccc atgagaacgg
    16261 ctttgaaccc tcagacacgg atctcttcag ttgcgcctgg gtgatgagag ccttgctgca
    16321 ctggtatcag gaagagctgg acctcaacga gctggaccaa tttctgcaag agcatcgcgc
    16381 cagccctttt ccagacaggc ctcatgccag gaaacacagc ttcctgggag tggtgctaag
    16441 ctggaaactg aaacacggcg accagcagaa taccggcatc gaaatcactg ccgctgaaga
    16501 agatggccgc tcagtgggta tctatctcag agacgatcct aagtccgagg gcagaaagtg
    16561 gtctttgctc gaccgctgtc tgtggaaatg ggccacgatc aactgcctca acctcagcga
    16621 aagctctggg gccgcggatc aatatattga caatcccggc acgatgattg tgatcgaacc
    16681 tgattatctg atcgacgtaa gcgcgctcgc ggaatgcgtt tcttccaaag gagtcaatcc
    16741 cgaactggct gtcctgggca gactgatttc agaggagagc actcagagcc tcgcgatcgg
    16801 atcggcagtc aacaacatct ttgacgaact tatgttcgat ccggacgctg attacaacca
    16861 gttattccgg gacagcatgg ccagagcgcc catccctctg gtggccctgg gcaaggaagc
    16921 agcgttgcag gttcacggta tcatcagaga tgagcatctg ccgcgtctga gggccatggc
    16981 cagattcgcg caccagcgtg acatgatgct ggagccctct tttatctctc ccaaatatgg
    17041 ccttcagggt cgcctcgatc tgctctacaa agacaaggga cgctactaca tcgtggagct
    17101 caagagcggc aacgctccgc aaacaggtat ctggcctcaa catctttatc aggtggtcgg
    17161 ctacaacctg ctgatcaaag aatgctatgg cgctgccaac cttggcacca gttccatcct
    17221 ctattccaaa ctgccggatg acaagctgcg gggcgtcacc aacctcatat cccaggaaca
    17281 ggaactcctg atgtgccgca accgcgtcat cgggatctgg aagcttctgt ccgaaaaccc
    17341 cgccaccttc ttcgactggc ttaagtctca cgaaggcaat cagctggcag gctttgtcaa
    17401 agagcgtctt catcgcgtgc agagcatcct gaacgcgctg gaccctccgg aatatgaatg
    17461 gtatctggaa caggttcgac tggctgtgag agaaacctgg tatgtcaaga tcggatcggg
    17521 aacccaccgt tccgaatccg gttatggata caacgccctc tggcaacaga gcagagaaga
    17581 aaagctgcgc cgctataaac tgctgcccgc tctgaaactg gtggcaatcg acaagaatct
    17641 cctgcgtttc aagctcgaag accgggaccg gatcagtgat ttcagagaag gtgacgtggt
    17701 ggtggcctac cgggaagacc gctccgtctc agcccaggag atcatcagag gagaactgat
    17761 cacgctgggt gaagaaactc tgcttgtgag ggcaagggga gtgctgaacc ccaccctgtt
    17821 tgccgatctg gacaggctgt ggtcgatcga acacgacctg ctggaaagca tgctgtttgg
    17881 cactctggcc tcgatcacgg gtttcctggc tgctccggag aataaacgcc gcaagctgtt
    17941 gggactgatg gcaccggaat tccaaactga tggggtagcc agcgctgaag acgtggaaag
    18001 cattgtagcc cagatcgatg ccgcgcggga ctattgtgtg gtccagggcc ctcctggcac
    18061 cggtaagacc tcaggcctga tcaccagttt catccgccgc ctctatcaaa actccgatca
    18121 gaccattctg atcctcagct tcaccaaccg cgccgtggat gaaatctgct ccaatctggc
    18181 caggcaccag atcccccata tccgcaccgg cagatctcaa aacgtggagc tcgagctgct
    18241 gggcaacctg atcagggaca agcgctttga cgagatcgag gaaattgtga agaccaatcg
    18301 cgtctgggtg gccaccgtgc agagctgtaa ctcctggatc aacgactggc tgaggatcat
    18361 acccggcatc ggcaccctga tcatcgacga ggcctctcag atcatcgagc ccaacatcct
    18421 gggcattatc gccagagcgg agcgctgtat cctgatcgga gaccagaatc aactgcctcc
    18481 gatcgtcgcg caaacagatg ccacctatag ctttaccgct gcggagctca gacagctctg
    18541 ctacagcggc tacaaccgtt ccctgatgga gaggctcttc ctgctcagcg agcaaaacgg
    18601 ctggccgcaa gcccgcttca tgctcaaccg ccattatcgc atgcatgacg agatcgcggc
    18661 gctggtgcag cagttttatc accatgacct gatcagctgc acaccccgcc agcaacgtac
    18721 ccttgaatcc agcgaaacca cccctgctta cctcaaaaaa cgggtcgtat ggattgactg
    18781 cccccctgcc actcatgcct actacgatga agagcagtgc gtcattattc atcggctcct
    18841 ggatacactc atacaattgg gcgagatcac agagctggcg accggagtgg gaatcgtggc
    18901 cccctaccgt gccatgatcc aggctttgag agctgaactg ggcagcccct tctccgccat
    18961 gaccatcgac acggtggagc gctttcaggg ctcggaacgg gatatcatca tcatcacgct
    19021 gcctttgaga gacaaggcgg acctcaggac cctggaagcg gtctcagacg accagaaggt
    19081 agaccgcaag ctcaacgtgt cactcagccg cgccaaagag aggctgatca tcctcggcaa
    19141 ctcgtcgctc tgtgcttcca gcggtcatta cgctttttta atagacagga tccgcgcttc
    19201 ccagactttg atcccatctt cacaactaat cgattaagta atgctataca aggagtaatc
    19261 aagatggcac aacccaatca acccaaccag atcaacatca agatcgacga aaaagtaggc
    19321 gaaggcacct atgccaattt ctttgtcatc accaattccc cctccgagtt tattatggac
    19381 tttggccgca tcctgcctgg cattccagac gccaagatct ataccagagt agtcagcact
    19441 ccctcccacg ccaagcagtt tctccagctg ctggaaaaga acatcgagac cttcgaaaag
    19501 caattcggcg aaatcaaagt gcagggcatg ccggaaaaca agccggtggg cttcaaacct
    19561 gatcgcggct gagcctcatt ataaaatcac ttgacggatg aatcggttca aaatctttgg
    19621 attgaaaccg atatttacaa ctactaaaat gaaatatgga gcataagcca atgaaaaaat
    19681 tctcatggcg cggactcacg atcctcatct tctgcttggg gactgcattt ttcctggctc
    19741 ctttggcgtt tgacggcctg ccttcctggt gggcttccca gaagctcaat ctgggtctgg
    19801 acctcaaagg cggaatgcag atcatcctcg aagtggatac ctccaagctg cctgccaatc
    19861 aggtcgcggg ggctgtggac aacaatattg agatcatccg caaccgcatc gaccaattcg
    19921 gcgtcgccga accgataatc cagaaaatgg gcgaaaagcg catcgtgatc cagctacccg
    19981 gcgtgaaaga ccatcttgcc gctgaaagcc ttgtaaaaaa gacagctatg ctcga
//
LOCUS       DAOSFN010000025        19801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2223_length_19801_cov_8.940190, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000025 DAOSFN010000000
VERSION     DAOSFN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 19801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19801
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2223_length_19801_cov_8.940190"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="PLO35_02990"
     CDS             <1..1843
                     /locus_tag="PLO35_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOH78661.1"
                     /translation="APSEGEGQAFESPQGRHKNLRNDMSSDVILTLTDISKSIGDKLI
                     FSNISLGIHQQEKIGIVGVNGCGKTTLLRVIAGEISATTGERIFRGKTKISYLPQDPV
                     FDPNIQVLDHLIPDHSVDPEHDDSYVYKSVLSRLGITEFHKPLGLLSGGQRRKTDLAR
                     ALVNDPDLLILDEPTNHLDMDTIEWLQDYLAKLKKTVIFVTHDRYFLDAVSTQIWEMD
                     KTKLFVSRGNYSEYLKGKLLRDVDAQRKETRRQAQLSKELQWLGRGARARATKPKHHV
                     DKVKELLSKSYLISNSELDISFQTDRLGKTILELHRLSKSYGGRELFRDIDHLFQPRE
                     RIGIIGPNGCGKTTLLRLITGEEEADLGSIKVGLNTHFAYYRQDEESFDPTMSVYDYI
                     AQFAEVIKTKDGTRLSASEMLQRFLFDGKMQQQKLGALSGGERKRLYLLKSLMFGANF
                     IILDEPTNDLDIRTLEILEDYLDAFDGCLLVVSHDRFFLDRTIDYLFVFEGTSIRKIP
                     GNYSDYLLIKRYQTEEEEERAQASMIMRPKRQTRGLNYNEQRELDQISVEIESLESRL
                     LDLETELSCPNLKPEDYSRISASQQELEARHSTLLERWLELSEKADL"
     gene            complement(2237..2314)
                     /locus_tag="PLO35_02995"
     tRNA            complement(2237..2314)
                     /locus_tag="PLO35_02995"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2277..2279),aa:Pro,seq:ggg)
     gene            2552..4462
                     /locus_tag="PLO35_03000"
     CDS             2552..4462
                     /locus_tag="PLO35_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOH78662.1"
                     /translation="MSLIQVRELSIEFGGNYILREISCTVEHNSRLGLIGPNGCGKST
                     LIKLMLGELNPTLGTVSRSSKAVIAYLPQNAQPDQDLSLEDYVKSARPDLMELRTQIE
                     KLSAQMDKSGGEALENQLHTLVDRYTSLGGYEWDNELKHVLLSLSFPETMWGQSIASL
                     SGGEQTRLCLAAMLLKPHDLLILDEPTNHLDIAMIGWLEKYLLKQDKPYLLVSHDRSF
                     LDNTVTGIYALRDGRLSVTKGNYSSWKEADGIARLSAERQFERQQKHIAATMEFVRKN
                     MAGQKTNQAKSRLKHLERMDLVERPLADRQLKIRMESSSRSGNDLFALKDLSFSAGGK
                     ELATRVNLIAHFQQRICVIGPNGCGKTTLLRMLIGKHAPDSGLLKTGASLKIAYYDQH
                     HVELDGSLTVMDTLWQLVPDAPRGYVLSWLARFGFRGDDVDKTVAVLSGGEKSRLYLS
                     VLIHSKPNLLIMDEPTNHLDIQMADALLEALQDFEGSIIFVSHDRWFLSRLATRYWVF
                     RKLLIGKEIITTVEELEGSSEAAIELSFAVPEAPRTAPMTRERKRRINPRILEMKHAE
                     IDQKQADLCNLRDELHTAQAELSLSSTYNDLARLKDLRAIQDELQNRIASTARVIEDL
                     EHEYLELLCEDE"
     gene            4455..5429
                     /locus_tag="PLO35_03005"
     CDS             4455..5429
                     /locus_tag="PLO35_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOH78663.1"
                     /translation="MSKRIGFTTSFPVEVVFAAGHTPIDLNNLFLASDSPQLMHAAEL
                     KGFPRTVCGWIKGNYSTALKSELDEVIGITQGDCSNGQSLLDMIHEEGLPVWRFAFPA
                     ERSRAEMDKELRRLEEHFEVDRTATLKAKARLDAIRAKLVTLDEWTWKERFVSGKENH
                     LWLVSSSDFNGDPDRFEEELEAFMNEASRRDPLPAKPRCAYLGVPPIYRDLYDRIGEL
                     GGDVVFNEVQRQFSMPHLAPDILDQYLLYTYPYSVKERLEDILPQLKRRDVDLVISYT
                     QSFCHLQIDNILLKKHIDLPFLTLEGDQPEPMDNRTLLRLESFFEVHA"
     gene            5426..6520
                     /gene="mnmA"
                     /locus_tag="PLO35_03010"
     CDS             5426..6520
                     /gene="mnmA"
                     /locus_tag="PLO35_03010"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOH78664.1"
                     /translation="MIRKVAVGLSGGIDSAMSALLLMEAGYEVIGLTMSFYDPASGIN
                     VASGKGCFGPNEALNILAARRVAEALGIEHHVIDLRTEFAGQVLDYYRSTYLAGQTPN
                     PCAKCNAFIKFGLLPQKARDLGIEFDLFATGHYVRRVQNPDTSRWQLFRGVDLSKDQS
                     YFLSLLRQEQLADTLFPLGEFSKSGVKEMAAQRGLDFLLSKAESQDFIQSDDHAGLFD
                     PALVKPGDLVDQEAKVLARHRGLVHYTIGQRKGLGISGMAEPVFVTRIDAERNQVVIG
                     PASQLYKKELVTRDFNWLSVSASQLPARAQAKIRQQHKAADCDLIPLGKNLLKLVFDE
                     PQLSITPGQIVAVYQGDLLLGGGFIASGSS"
     gene            6712..9987
                     /locus_tag="PLO35_03015"
     CDS             6712..9987
                     /locus_tag="PLO35_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78665.1"
                     /translation="MLQNLSAMLSQTGPGVQMPPVSSGPLLREGEKRFVSVLFADVKG
                     FTSMSEILAPDLVQMLLDQLMLSFSVEVERYGGYVDKYEGDRIMAHFGSKSFLEQNSR
                     RAVYAGLGMIGVITSFNQIRHQIPELEAMPSDLAIRVGINSGMVATGRVGMKREGDFT
                     VYGDAVNLASRMEENGLPMRVLLPETVKSELEDYFEFSFFGEINVKGKAEAVRTWLVD
                     QPLADAGSRRLSSTPFLGREKELKLLREALNPGSRLKLLHLEAEAGMGKTRLLREFVC
                     EDQIDEYAGTDADQQVSVFHLEVSPLARIPYSYFAGLIKTWCSITDLQDNDSVIARLN
                     RGLDNLDGLQSDEKSQLISCLMIVLGRAASDQLAWGSLEEQRTAINRSLMNLIRALSA
                     SRSKTVLIFDDLHFADEASLHTLAFLIRSFHDLPELPLLFILVARTACFPKDLIPRDI
                     MTPELSLEAFSEAEIKEMILRCLPEIELPLAVTNALISRSAGNPLYLEFWLEGIRAQL
                     KNNAYPQADEIIIPDGISSTLLWRLNWLDQPAREQMQRAAVCGDVFAPGLLYELELRF
                     GQSSDPGATLRRAEQLGLICFDGSTCSFRHHLIWEAVYGTILEANRVVLHAEAAAAIE
                     RLYPGGLEPYYMRLADHYLAAKARDKAAHYLIKAESQARSLFMGKRVVACCRKLLKIL
                     PPQEQPPVRVRLATQLMDLGQVREAEKELSKLEIDQVSDREEYIHTRLRVLQARQGAS
                     VAKSWLLEQKELPGCPALRISLIDLKRLCGDADDLQSEADELQSSMSTPVQRTRLHNT
                     LGLWHKDRGRYQQAQECFEAARTQAADNKPLRRIVLHNLANVLTRTGNRSQALPIYLE
                     AIKLAEELGDEGGLAKLQGDLGGLYLRSGDRELAVRAFQASSMAARMVGDKLVEANAL
                     YNLAVAEYDSGRLAEARCWLDKCLSLYRSLKSSTGMVHARDLLGDILYSEGQASQAAR
                     VYGINLKAQKRGGDLEGLAHSLGNLANIAADQGNYNKALALYTRQAQILRECKDPEGE
                     GKALYNLAILADESGKREMAISYLKDALRLFEAADLQTYLPEARAFMNDLTVNEKETR
                     EER"
     gene            9984..>10873
                     /locus_tag="PLO35_03020"
     CDS             9984..>10873
                     /locus_tag="PLO35_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOH78666.1"
                     /translation="MNLRKVINICLIAMLQLGVAVSIAGRSTPRIVTQKLACQSPINI
                     MDIVTNTTGTSPSYVIRADVLETAGEELGSDLGTSDANLYIRKLGNGMTVPYYIGAGV
                     QLSQFITQWQAGQTLRLTITYIASGASSFQDITIPAGTANVLIQEPVMIIPPYPIIDA
                     PTGIDASLLPSGILRISWDAVTGATGYNLYRSIDGGNSFTMVAQTANDYYDDAVTLPV
                     WELRIYHYRVSAVSGRVESEPSEILSVALDRIASVGNSLLGIPLGAGVWVSEWVQGQS
                     FMIDSVSYWNANIQQWMTAF"
     assembly_gap    10874..10973
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11339..15166
                     /locus_tag="PLO35_03025"
     CDS             11339..15166
                     /locus_tag="PLO35_03025"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78667.1"
                     /translation="MEVIRSATSVTNVGDLVFTMDDTANRAALTAIYNACGGANWTNN
                     TNWLSPLPLSEWYGVQTYSGQVTILDLSNNNLIGDLPPAVNTLTSMHVLNLSHNSLLT
                     LCIISGLLPDCAINVSFNQLHFYSFDINVFAPGIWTRFPQNPCYYPQTINATQGATVE
                     MHSGISGNNLTYNWTKDGAFLTTTASGNLILNDVQPGQAGIYGCSVYSNDWAVFIERH
                     PITLTVMSVNHPPVIDMPAVLHINQNESLSFDIYQYASDPDGDTLLPSVTGNQFISAV
                     VSSNIVTLYPSENWTGTETITILVVDDVVGMQWWAEDTVSIVVSNPLEIDFDTDSSLN
                     NNIVMDSPATVPVFTATCDAPYSLVSWAWDFDNDGITDSTLPNPQPQYTSTGLKDVRL
                     TASDGIHVSSLLKPSFITVLPGEYIPPAVVDEDLIFYSNNVYNLGGELQIDTGVNLIV
                     QPNAEVNMLTQQQLVINGNIQASGASFQASRVDEWGGIVLNSGASGSSLVNLQISGAS
                     IPLVLNDCSPFINNLVLEAEPGTRNPGPAIRINGSSAPLIQSLQTHGFATAIKAHRDG
                     AGAVTLNIDSVLLNRGDEVPSSTDIGIEIQGDYWFEAQDVRIQGYPRGIGIQGTQPTL
                     GRARLTGSRVVQNESNRDPGTAISIAGVGFIQIMADTLIGFDNAIVVSNGGTASSCEV
                     SSCVISKQAYGANSVGISVSGFQSLRADSLLVSNYGTAISTDGGSSRSIGFNQLLNCG
                     TGILTGSPASYVQVRNNIIRRDQAYPGTQDQPALNLTAANAEITGNTVFAHPTSLRLN
                     GGTAATLVNNIFWPQQEGPDPIQIMGTSTLSATYNDIRLTVGAYPGTGNQNRDPRFVD
                     PGTGNLNLGIRSRCIDAGDPGWPLDPDGSRADLGALYFDQTNMSYETDFYCDIQAGPH
                     PLAVQFYDDSAFSASSWNWDFNGDGTIDSTLRDPLWTFEQPGSFSVSLSSSDGSNTAT
                     VTYDDLISVWNTAPEVLQPIEAQNWPEDFGLWVCQLDAFFNDADGDSLSYSVALSEPL
                     VSWEITGSDLRIHSIQDLFGALTVTVTADDGYSEARAKNGLRVECSLSFNVTIDPVND
                     PPELISAFPEQSLLYIAEGDEIDFNLEVVDVDSPVAYIWLLDNVDQNNNGPEWSHVFD
                     EQGYHSVSVTISDGVNSFLWGWGLQVQSSPSDDPLVPAFTALLPNTPNPFREGTSIHY
                     AINRQGQFEMAIYNSRGQLVKRFQNCPGEAGWHHQYWNGCDSHGRKVSAGVYLVRMTH
                     EGKSYTRKVLLVR"
     gene            15365..17059
                     /locus_tag="PLO35_03030"
     CDS             15365..17059
                     /locus_tag="PLO35_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78668.1"
                     /translation="MTKSSLFSSVRAILTVLVVILLVSACDDDNPTNPTVNTGVVSGT
                     VFKAARTVLPGVTVSIGTQTTETDADGKFFIYGVNAGTSVIVDFEKEGYIANQKVITV
                     NKDKTTYIDSTLRIPLATTFASTVGTMLVDGYTEITIPENAFMVGSTPFTGNVRAEYR
                     YYDPTNADNLNAFPGSFSGVQTDGTETMFESYGYISASFTDAQDPNRQLQLAAGKTAS
                     ILSYIPTSLLANAPATIPMWYYNESTGKWMEDGVATKVGNYYQGEVTHFSYWNFDHPI
                     VVDDQSTLTGRVVSAESKAPILGAQVVATGVDYAGYTRVYSDANGLFSITVKASAQVT
                     IKAYSGINSSPVSPVINTPVSGGTQDVGDISIPDLSFTLTGKLVDTSNQPISAGYGQM
                     TQVNPPDGVNPFNAWIGLDANGNFSIQEAYSGSLTSFPVQFSINQRGNLYSSSITFTV
                     PQPGQVYNFGTIVMREGGKLNGRAKDHLGNWIADSWVSFMQEGATGEGNHVSGSTDAN
                     GYFTLTGPPSTNLTNMRGSVYVNQTNLVSPLMSLNFPASGQEGNIGTVIFSPQSKR"
     gene            complement(17133..18485)
                     /locus_tag="PLO35_03035"
     CDS             complement(17133..18485)
                     /locus_tag="PLO35_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOH78669.1"
                     /translation="MVNPQVFRQYDIRGIVDEEINTETYYLIGRGFGTYMRDKGLKTI
                     VVGGDARPSSPGLMKELIRGLLETGCDVTDIGMVATPVLYYSIWKLKMDGGAMVTASH
                     NPSQYNGCKLNLGLGSVYAEELQKVLGIIQSGKFQTGAGNLVQDDSLNADYIDYITAN
                     IKLERPVKVIVDGGNGMGGPYLPEILRRLGCEVIEMYCEPDGTFPNHHPDPTIEKYMV
                     DLSRAVVAAEYELGLGLDGDADRIGVVDEKGKMLFGDQILNILARDYLIDNPGKTIIA
                     DVKCSKNLYDDIRARGGVPMMYKTGHANIKMKMKEIGVEFAGEMSGHVFLADRYLGFD
                     DAIYVSCRFIEIVSKTSAPVSSYLADQPKMYNTPEIHVKCADDRKFEVVAAVCDEFKR
                     EGYDVTDIDGARITFADGWGLVRASNTTPVLVTRYEAIAEERMHQIKELVEGKINKYI
                     "
     gene            complement(18578..19174)
                     /gene="rdgB"
                     /locus_tag="PLO35_03040"
     CDS             complement(18578..19174)
                     /gene="rdgB"
                     /locus_tag="PLO35_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOH78670.1"
                     /translation="MRSLLIATNNPDKFREISALLSDLPFHLFKMEDFPQLSAVEEDR
                     ATIEGNAAKKALETARAADLLCLADDTGLFIDALGGEPGVFAARWAGEGCTYRDNRLK
                     ALQQMSGVSDRAASFRTVVALAAPDGIIGCEEGRVQGIITTQERGQRGFGYDAVFEVN
                     GTGLTYAEMSDSDKNTLSHRALAILAIRPLLERILSLR"
     gene            complement(19175..19699)
                     /locus_tag="PLO35_03045"
     CDS             complement(19175..19699)
                     /locus_tag="PLO35_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOH78671.1"
                     /translation="MNEDILEGREIPEEEKEKIDDRVVDMDPDKLKFYEDLRRKAKDW
                     TGQKTGKMGGKLSEYLFLLPDFFIMLTRLMLDKRVPGKRKLMVGGIVAYMMLPFDIIP
                     DFIPIIGHVDDLVLVVMGLNMVLNDTDPKVLADNWSGEGEVLQQIQKITGVAEGFLDK
                     NVLSKIKKWLGKIS"
BASE COUNT         4700 a         5304 c         5260 g         4437 t
ORIGIN      
        1 ggcaccctcc gaaggtgaag gtcaggcgtt cgaatcgcct cagggacgcc ataaaaatct
       61 ccggaacgat atgagcagcg acgtcatcct caccctgaca gacatttcca agagcatcgg
      121 cgacaagctg atcttctcga atatctctct gggtattcac cagcaggaaa agatcggcat
      181 cgtgggtgta aacggctgcg gcaaaaccac tttgctgaga gtgatcgccg gagagatttc
      241 cgccactacg ggtgagcgca tcttccgcgg caagacaaag atatcctatt tgcctcaaga
      301 tcccgtcttc gatcccaaca tccaggtgct ggaccacctc atccccgatc actccgtcga
      361 tccagaacat gacgacagct atgtctataa atccgtcctc agccggctcg gcatcacaga
      421 gtttcacaaa cccctggggc tgctttccgg aggccagcgt cgcaaaacag atctggccag
      481 ggctctggtc aacgatcccg acctccttat cctcgacgag cccaccaacc acctggacat
      541 ggataccatc gaatggctgc aggattacct cgccaagctc aaaaagacgg tgatcttcgt
      601 tacccacgac cgctatttcc tggatgccgt cagcactcag atctgggaga tggataaaac
      661 caaactcttt gtaagcagag gcaactacag cgaatatctg aagggcaaac tgctgcggga
      721 cgtggatgcc cagcgcaagg agactcgtcg ccaggcccag ctttccaaag aactgcagtg
      781 gctgggacgc ggtgccagag ctcgcgccac caagcccaaa catcatgtgg ataaggtcaa
      841 ggaactgctc agcaaatcct atctgatctc aaactccgag ctcgatatct cctttcagac
      901 cgatcgcctc ggtaagacca tcctggaact gcatcgcctc tcgaaaagtt atggcggcag
      961 ggagcttttc agggatatcg accatctctt ccaacccaga gagcggatcg gcatcatcgg
     1021 ccccaacggc tgcggtaaaa ccaccctcct gaggttgatc accggtgagg aagaagccga
     1081 tctgggcagc atcaaggtgg gcctcaacac ccattttgcc tactaccgtc aggatgagga
     1141 gagctttgac cccaccatga gcgtctatga ctacatcgcc caattcgcgg aagtgatcaa
     1201 gaccaaagac ggcacccggc tcagcgcttc cgaaatgctg cagcgctttc tctttgatgg
     1261 caagatgcag caacaaaagc tgggagccct ttccggaggc gaacgcaaac gcctctacct
     1321 gctcaaatcc ctgatgtttg gcgctaattt catcatcctg gacgaaccca ccaacgatct
     1381 ggatatccgc acgctcgaaa tcctggagga ctatctggat gccttcgacg gttgcctgtt
     1441 ggtggtatcc cacgatcgct tttttctgga ccgcaccatc gactatctct ttgtgtttga
     1501 aggcacttcc atccgcaaga tccctggcaa ctactcagac tacctgctca tcaaacgcta
     1561 ccagaccgaa gaggaagaag aacgcgcgca ggcctcaatg atcatgcgtc ccaaacgtca
     1621 gaccaggggc ctcaactaca acgagcagcg agaattggac cagatcagcg tcgagatcga
     1681 gagcctggaa agccgccttc tcgacctcga aaccgagctc tcatgcccaa atctcaaacc
     1741 cgaggactat tcccgcatca gcgcttcgca acaagagctg gaagcccgtc attccaccct
     1801 cctggaacgc tggctggaac tcagcgaaaa ggctgattta tagggtcgga ttccaatccg
     1861 gcgggcgcat cttcacttca gagctactga ggttctttag tcactacaga ggccacggag
     1921 ttgacggaga aagacacaga ggttagttta ttatgtgctt tagcccgctt gctcccctcc
     1981 tgatcccctc ctatgccaaa gcataggtga tgtaaaggtg gggcataggt ggagtaaagg
     2041 aggcgataaa gaaatagcat atttgcagac acataccttg atttggggat caaaaaaatc
     2101 gatggaacag gtcattgtgc cggccgataa gtatttggga atgaatgcat tataatcgca
     2161 ggaaagcaca gggagcagag caacctcgcg gataaagcaa agcggcgacc accgattggt
     2221 gaccaccgct aaactatggt cgggatgaca ggatttgaac ctgcgaccac ctgaacccca
     2281 ttcaggtacg ctagccgggc tgcgctacat cccgaacccg ttggagacct taaaaattga
     2341 gacggctttt ttgacaagta aaatctgtgc ttgacatcag acctgaggac ggaaatgtgg
     2401 cctcagcgct ttgtgggaca ggtgttaatt gaaaattgag aattgagaat tgagaattgg
     2461 ctggcgctga gcatgaagca aggaaacaag ataacggcaa gctaaaaggc cattcttatc
     2521 aatggatata tagccggtcc ggaaagggaa aatgagccta attcaagtcc gcgagctcag
     2581 tatcgagttt ggcggcaact acatcctgcg ggagatttcc tgcaccgttg agcacaacag
     2641 ccgcttagga ctgatcggtc ccaacggctg tgggaagagc acgctcatca agctcatgct
     2701 gggagagcta aaccccaccc tgggaactgt ctcccgctcc tcgaaggccg tgattgccta
     2761 cctgcctcaa aacgcgcagc cggaccagga cctcagcctg gaggactatg tcaaatctgc
     2821 ccggcccgat ctgatggaac tgcgtacaca gatcgaaaaa ctttcagccc agatggacaa
     2881 aagtggggga gaagctctgg aaaatcagct gcatacgctg gtggaccgtt acacctctct
     2941 gggcggctac gaatgggaca acgagctcaa gcacgtgctg ctctcgctgt cctttcccga
     3001 aacgatgtgg gggcaatcca tcgcttcatt aagtggagga gaacagaccc gtctctgcct
     3061 ggccgccatg ttgctcaagc ctcatgacct cttgattttg gacgagccca ccaaccatct
     3121 cgacatcgcc atgatcggct ggctggaaaa gtatctgctc aaacaggaca agccctatct
     3181 gctggtctcg cacgaccgca gttttctgga taacaccgtc acaggcatct atgccttgcg
     3241 ggatggccgc ttgagcgtga ccaaaggcaa ctattcctcc tggaaagaag cggacgggat
     3301 cgccaggctg agtgccgaac ggcagtttga acgccagcag aagcacatcg ccgccactat
     3361 ggaatttgtg cgcaaaaaca tggccggcca gaagaccaat caggccaaga gccgccttaa
     3421 gcacttggag cgcatggatt tggtggagcg tcccctggca gacagacaac ttaagatacg
     3481 catggaatca agttcccgca gcggtaatga tctctttgcg ctcaaagacc ttagcttttc
     3541 cgcgggcggc aaagaactgg ccacccgggt gaacctgatc gcgcatttcc aacagaggat
     3601 ctgcgtgatc ggccccaacg gctgcggcaa gactactctc ttgcggatgc tgattggtaa
     3661 acacgctccg gacagcggtt tgctgaagac gggcgccagc ctcaaaatcg cctattacga
     3721 tcagcatcac gtggaactgg atggctccct caccgtgatg gacacgctct ggcaactggt
     3781 acctgacgcc cccaggggct atgtgctctc ctggctggcc agatttggct ttcggggcga
     3841 tgatgtggat aagacggtag ccgtcctgag cgggggcgaa aagagccgtc tttatctcag
     3901 cgttctgatt cactcaaaac ccaatttgct gatcatggac gagcccacca atcacctcga
     3961 tatccagatg gcggacgcgc tgttggaagc cctgcaggat tttgagggca gcatcatctt
     4021 cgtgagccac gaccgctggt ttctctccag gctggccact cgctactggg tgtttcgcaa
     4081 gctcttgatt ggcaaggaga tcattaccac agtggaagag ctggaaggta gcagtgaagc
     4141 agccatcgag ctcagttttg cggtccctga agctcccaga actgctccca tgaccagaga
     4201 gcgtaaacgc cggatcaatc cacgcatcct ggagatgaaa catgccgaga ttgatcagaa
     4261 acaagcggat ctgtgtaacc tcagggatga actccacacc gcccaggcag agctttcgct
     4321 ttccagcacc tacaacgacc tggcgcgcct gaaagacctg cgcgctatcc aggatgagct
     4381 gcaaaaccgc atcgcttcaa ccgccagggt gatcgaagac ctggaacacg aatatctgga
     4441 gctgctctgt gaagatgagt aaaagaatcg gtttcaccac cagttttccc gtcgaagtgg
     4501 tcttcgcggc tggccatact cccatcgatc tcaacaacct ctttctggcc tccgattccc
     4561 cccagttgat gcacgcggcg gagctaaaag gctttcccag aacggtgtgc ggctggatca
     4621 aaggcaacta cagcaccgcc ctcaagtctg agctggacga agtgattggc atcacgcagg
     4681 gcgattgctc caacgggcag tcgctactgg acatgatcca tgaggaaggg ctgccggtgt
     4741 ggcgttttgc ctttccggcg gagcgttcgc gagcggagat ggataaagag ctgcgcaggt
     4801 tggaagagca ctttgaggtg gacaggaccg ccactctgaa ggccaaagcg aggctggacg
     4861 cgatccgcgc caaacttgtc actctggatg aatggacttg gaaagagcgc tttgtgagcg
     4921 gtaaagagaa ccatctctgg ctggtttcgt catcagactt taacggtgat ccggatcgct
     4981 ttgaggaaga gctggaggcc tttatgaacg aggcttccag gcgtgatcct ctgcccgcga
     5041 agccacgctg tgcctatctg ggtgtgccgc ccatctatcg tgacctctat gaccggatcg
     5101 gcgaactggg cggggatgtg gtcttcaacg aagtgcagcg ccagttttcc atgccccatc
     5161 tggccccgga cattctggac cagtatctgc tttacaccta tccatatagc gtcaaagaac
     5221 gcctggaaga cattctgcct cagctcaaac gtcgtgatgt ggacttggtg atcagctata
     5281 cccagtcttt ctgtcatctt cagatcgata acatcctgct caagaagcat atagacctcc
     5341 ccttcctgac tctggaaggc gatcagccag aaccgatgga caaccgcacg ctgctgaggc
     5401 tggagagctt tttcgaggtg cacgcgtgat ccggaaagtg gctgtaggcc tcagcggagg
     5461 gatcgattcc gccatgagtg ctctgctcct gatggaggcc ggttatgagg tgattggcct
     5521 caccatgagt ttctacgatc ccgcttcggg gataaatgtt gcttccggca aggggtgttt
     5581 cggtcccaat gaggcgctta acatcctggc tgccaggaga gtcgctgagg ccctcgggat
     5641 tgagcatcac gtgatcgacc tcagaactga gtttgccggc caggtactgg attactaccg
     5701 ctccacttat ctggctggcc agacgcccaa cccctgcgcc aagtgcaacg ccttcatcaa
     5761 gtttggccta ttgccccaaa aagcgaggga tttgggcatc gagtttgacc tttttgccac
     5821 cggacactat gtacggcggg ttcagaatcc cgatacctct cgctggcagc tgttcagagg
     5881 agtggacctg agcaaagatc aatcctattt tctgagcctg ctgaggcagg aacagcttgc
     5941 cgacacgctg tttccgctgg gtgaattttc caagtccggg gtaaaagaga tggcagctca
     6001 gaggggactg gacttcctgc tgagcaaagc tgaaagccag gatttcattc agagcgatga
     6061 tcacgccggc ctctttgatc ctgcgcttgt gaagcctggc gacttggtag atcaggaagc
     6121 taaggtcctg gcacggcatc gcggtctggt ccattatacg attgggcagc gtaagggatt
     6181 ggggatcagt gggatggctg agcctgtctt tgtgacccgg atcgacgcgg agcgcaatca
     6241 agtggttatc ggacctgcca gtcagcttta caaaaaagaa ctcgtaacgc gggatttcaa
     6301 ctggctctct gtgagtgcgt cacagcttcc tgccagggcc caggccaaaa tccgccagca
     6361 gcacaaagcg gccgattgcg atctgatccc tctgggaaaa aacctcctga agctggtgtt
     6421 tgacgagcct cagctttcga tcacaccggg ccagatcgtg gctgtctatc agggtgatct
     6481 gctgctggga ggaggcttta tagcctccgg gagttcttag cctgaccagc aagccataga
     6541 cttcactgct aacaaccagc cgggtgcctg agataaagtt gttgtggcca taaatatatt
     6601 gacagatccc ggctatcctc tgcaaggttc tttcatcgtc aacaaagctc attaaatcgg
     6661 cacaacttat cacgttctga aatacttatc tggcctctgc aaggagttct tttgctgcaa
     6721 aaccttagtg ccatgctctc tcagactggt cctggcgtcc agatgccgcc agtgtcatcg
     6781 ggaccgttat tgcgtgaagg ggagaagcgc tttgtgagcg tgttgtttgc cgacgtcaaa
     6841 ggctttactt ccatgagcga aatcctggcc ccggacctgg tacagatgct gctcgaccag
     6901 ctaatgctga gcttttcagt cgaggtggag agatatggcg gctatgtgga taagtatgag
     6961 ggcgacagga tcatggccca ctttggctcc aaatcctttc tggaacagaa ttcccggcgg
     7021 gctgtgtatg cggggctggg catgatcgga gtcatcacgt cctttaatca gatcaggcat
     7081 cagatacccg agttggaagc catgccctcc gatctggcca tccgggtggg tatcaacagt
     7141 ggtatggtgg ctaccggacg ggtgggcatg aagcgggaag gtgattttac cgtctacggc
     7201 gatgccgtca atctcgcctc cagaatggaa gaaaacggtc tgccgatgcg ggtcctgctg
     7261 ccagagaccg tcaagtctga actggaagac tactttgagt tcagtttctt tggagagata
     7321 aatgtgaagg gcaaggctga agcggtgcgc acctggctgg tggaccagcc tttagcggac
     7381 gcggggtcgc gccgtctgtc ttccacgcct tttctgggca gggaaaagga actgaagctc
     7441 ctgagggagg ccctgaaccc tggatcgcgt ctcaagctgc tgcatctgga agcggaagcc
     7501 ggaatgggga agaccagact gctgcgagag tttgtctgtg aggatcagat tgatgagtat
     7561 gctggaactg acgccgacca acaggtttcg gtttttcatc tggaagtctc tcccctggcc
     7621 agaattccat actcctattt cgcgggcctg atcaagacct ggtgctcgat cacagatctt
     7681 caggacaacg atagcgtgat tgccaggttg aaccgtggcc tggacaacct ggatggactc
     7741 cagagcgacg aaaaatctca gctgataagc tgcttgatga tcgtgctggg gcgtgctgct
     7801 tctgatcaac tggcatgggg cagtctggaa gagcagagga cggccatcaa ccggtctctg
     7861 atgaacctga tcagagcctt gagtgcaagc agaagcaaga ccgtcctgat atttgacgat
     7921 ttgcattttg ccgatgaggc ttccctgcat acgctggcct tcctgatcag atcattccat
     7981 gatcttccgg aactgccgtt gctgtttatt ctcgtggcca ggacagcatg ctttcccaaa
     8041 gacctcatac cccgggatat tatgactcca gagctaagcc tggaggcttt tagcgaggct
     8101 gagatcaaag agatgatctt gcgctgtctt ccggaaattg aactgccgct tgcggttaca
     8161 aacgcgttga tctcacgctc ggcaggtaat cccctctatc ttgagttttg gctggagggt
     8221 atcagggccc agctaaaaaa caacgcttat ccccaagcgg acgaaatcat catccccgat
     8281 ggaatcagct ccaccctgct gtggaggctc aattggctgg atcagcctgc cagggagcag
     8341 atgcaaagag ctgccgtctg tggagatgtc ttcgctcccg ggctgctcta tgaactggag
     8401 ctcagattcg gccagtccag cgatccggga gctactctga gacgggctga acagcttggc
     8461 ctgatctgct ttgatggctc cacttgcagc tttcggcacc acttgatctg ggaagctgtc
     8521 tacgggacga tcctggaggc caacagagta gttctgcatg ccgaagcggc cgcggcgatc
     8581 gaaagacttt atccaggcgg tctcgagccc tattacatgc ggctggccga tcattatctg
     8641 gccgcaaagg ccagagacaa agccgcgcac tatctcatca aggccgaaag ccaggctcgc
     8701 agtctgttta tgggcaaacg cgtagttgcc tgctgccgca aactactcaa aatcttgcct
     8761 ccgcaagagc agccgccagt gagagtgcgg ctggccaccc agttgatgga cctgggccag
     8821 gtccgcgagg cggaaaaaga gctctcgaag ctggagattg atcaagttag cgatcgggag
     8881 gagtacatcc acaccaggct gcgggtgctg caagcacggc aaggagcttc ggtagcaaaa
     8941 agctggctgc tggagcagaa agaactgcct ggatgccccg ccctcaggat atctctgatc
     9001 gatttgaaga ggctttgcgg ggacgcagac gacctgcagt ctgaagctga tgaactgcag
     9061 tcaagtatga gtactccggt gcaaagaaca cggctgcaca acaccctggg cctctggcac
     9121 aaagaccggg gacgttatca acaggctcag gagtgttttg aagcagcccg tacgcaagca
     9181 gctgacaata agcctctgag acgcatagtc ctgcacaatc tggccaatgt cctcacccgg
     9241 actggaaaca ggtcccaagc gctgcctatc tatctggagg ccatcaagtt ggctgaagaa
     9301 cttggagacg agggtggact tgcaaagctg caaggcgatc tgggaggctt gtatctgcgg
     9361 agcggcgatc gtgaactggc ggttagggct tttcaggcga gctccatggc agccagaatg
     9421 gtgggggata aactagtgga ggcaaacgcg ctctacaatc tggcagtagc tgagtatgac
     9481 agtggcaggc ttgcggaagc gaggtgctgg ctggacaagt gcctgtcgct ctatagaagc
     9541 ctgaaaagca gcactggaat ggttcacgcc cgtgacttgc tgggagacat cctttacagt
     9601 gagggacagg catcacaagc ggccagagtt tatggcatca atctcaaagc ccagaagcgc
     9661 ggtggagatt tggaaggcct ggcccacagc ctcgggaacc ttgcaaatat cgcggctgat
     9721 caaggtaact ataacaaggc attagcttta tacacccgtc aggctcagat cctgagggag
     9781 tgcaaagatc cagagggaga gggtaaagct ctttacaatc tggcgatcct ggcagacgaa
     9841 agtggcaagc gggagatggc gatcagctat cttaaagacg cgctgcgttt gtttgaagcg
     9901 gctgatctgc agacctatct acctgaagcc agagctttta tgaacgatct gacagtaaat
     9961 gaaaaggaaa caagggagga acgatgaacc tcagaaaggt gatcaatata tgcctgatag
    10021 ccatgctcca gcttggcgtg gcggtttcaa tcgcgggaag gtccaccccc aggattgtga
    10081 cccaaaagct ggcttgccag tctcccatca acatcatgga tattgttacc aatactacag
    10141 gaacttcacc gtcctatgtg atccgcgctg atgttttgga gactgctgga gaggaactgg
    10201 gctcagacct cggcaccagc gatgccaatt tgtatatccg caagctgggc aacgggatga
    10261 cggtaccata ttatatcggc gcgggggtgc agctgtcgca attcatcact cagtggcagg
    10321 ccggccagac tctgaggctc accatcacct atatcgccag cggggccagc agttttcagg
    10381 atatcaccat ccccgccgga actgcaaacg tcctcatcca ggaacccgtg atgatcattc
    10441 ctccctatcc cataatcgat gcccccactg gtattgacgc ctcgctgcta cccagcggga
    10501 ttttgagaat atcctgggac gcggtcactg gcgcgacggg atataatctc tacaggagca
    10561 tagatggtgg caacagcttt acgatggtgg cccagactgc caatgactac tatgatgatg
    10621 ctgtcacgct gccggtctgg gagctcagga tctatcatta tcgggtctcg gctgtgagcg
    10681 gcagagtgga atccgagccc agcgagattc tcagtgtggc tctggaccgg atcgcgagcg
    10741 tgggtaacag cctgcttggc atacctttgg gtgctggcgt atgggtctca gagtgggtgc
    10801 agggacagag ttttatgatc gactcggtaa gctattggaa tgcgaatatc cagcagtgga
    10861 tgacagcgtt tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttcatct
    10981 atagcacggt agcctatccc aactggtatg ccccaagagg aggcgtgaaa tgaataaggc
    11041 attgctccta ttggttttcc tgtttatagt ggtcggcata tcctcgcaat ctctgcctca
    11101 catgatcgtg ggaaaggcat ggaaccaagc tggcgtgcct cacagcacta taaggatcag
    11161 actaagcgtt cagggcgtta gttattatta caactggccg ggtcaaactg ataaccttcc
    11221 gggcagcatc acaacgtctc ccgcttttgc tatccagacc tcagcaattt ccggtttcca
    11281 ggctccagga gcaacggcaa cactgacgct taagggtgac aacgatgcag aatcaagcat
    11341 ggaagtgatc cgttctgcca catctgtaac taatgtgggg gatcttgtct tcaccatgga
    11401 cgataccgca aaccgggctg ctcttaccgc tatctacaac gcctgcggag gcgcgaactg
    11461 gacaaacaac acaaactggc tgtctccttt gccgctgtca gaatggtatg gagtgcagac
    11521 ctattcaggg caggtcacaa tccttgatct ttccaataac aacctcattg gagatttgcc
    11581 gccggcagtg aacactctga cgtccatgca cgtactgaat ctgagtcaca acagcctttt
    11641 aacactttgc atcatcagcg gtttgttgcc agactgtgcg atcaatgtca gcttcaatca
    11701 gctgcatttt tactctttcg atatcaatgt ctttgcaccg gggatctgga cacgctttcc
    11761 gcaaaatccg tgttattacc cccagaccat caatgcgacc cagggtgcca cagtagaaat
    11821 gcacagcggg atttcaggca acaacctgac ttacaactgg acaaaagacg gagcttttct
    11881 gacaaccacc gcttcaggaa atctcatcct gaacgatgtc cagccaggcc aggcaggcat
    11941 ctacggttgc tctgtctaca gtaatgactg ggcggttttt atcgaaagac atccgattac
    12001 tctgacagtg atgagcgtaa atcatccccc tgtgatcgat atgccggcgg tgctgcatat
    12061 caaccagaac gagagcctca gcttcgacat ctaccaatat gcttccgatc cggatggcga
    12121 tactctcctt ccctctgtaa ctggaaacca gtttatctca gccgtagtca gctctaatat
    12181 tgtaaccttg tatccctcag agaattggac tggaacggaa actatcacca tcctcgtggt
    12241 ggatgatgtg gtgggtatgc agtggtgggc tgaagacaca gtaagcattg tggtctcgaa
    12301 cccgctggag atagactttg acacagacag cagcctaaac aacaacatcg tgatggattc
    12361 tcctgctacg gtgccggtct tcactgccac ttgtgatgct ccctacagcc tcgtttcatg
    12421 ggcgtgggat tttgataatg acgggatcac ggattccact ttgcccaatc cccagcctca
    12481 atatacaagc actggcctca aagacgtaag gctcacagcc agcgacggca tccacgtgag
    12541 cagcctgcta aaaccaagct tcataactgt cttgccgggt gaatacatcc ctcccgccgt
    12601 ggtggatgaa gacctgatct tctattccaa caacgtctat aacctgggtg gagagcttca
    12661 gatcgatacg ggggtgaacc tgatcgtgca gcccaatgcc gaggttaaca tgctcaccca
    12721 acagcagttg gtgatcaacg gcaatatcca ggccagcggg gcaagttttc aagccagtcg
    12781 cgtggatgaa tggggcggaa tagtcctcaa ctccggagcg tctggcagct cgctggtgaa
    12841 tctgcagatc tcgggagcat ccatcccatt ggtgctcaac gactgcagcc cttttatcaa
    12901 caacttggtt ctggaggctg agcccggcac cagaaatccc ggtccggcca tcaggatcaa
    12961 cggcagttcc gctccgctga tccaatcact tcagacccac ggttttgcga ctgcgatcaa
    13021 ggcccataga gatggtgcgg gagcggtaac attgaatatc gacagtgtcc tgctgaatag
    13081 gggagatgag gttccatcga gcacagacat cggaatagaa atccagggtg actactggtt
    13141 tgaagctcaa gacgtcagga tccaaggcta tccacgcgga atcggtatcc aaggcaccca
    13201 acccacgctt ggcagggcca ggctcacagg cagcagagtg gtgcaaaacg agtctaaccg
    13261 tgatcccggc accgccataa gcattgcagg agtgggattt attcagatca tggccgatac
    13321 cctgataggc ttcgacaacg ccatcgtagt tagtaacgga gggaccgctt ccagttgcga
    13381 agtcagctcg tgtgttatct ccaagcaggc atacggcgcc aattcggttg ggatcagtgt
    13441 gagcggcttt caaagcctga gggcggactc tctgctggtt tctaactacg ggaccgccat
    13501 cagtactgac ggaggttcaa gtcgcagcat cgggttcaac cagctactca attgcgggac
    13561 agggatttta accggcagcc ctgccagtta tgttcaggtc aggaacaaca tcatcaggag
    13621 agatcaagct tatccaggaa cgcaggacca gcctgccctc aacctgacag ccgccaacgc
    13681 tgagatcact ggcaacaccg tctttgccca tccgacctca ctgaggctga acggtggaac
    13741 agccgccact ctggtcaata acatattctg gccccagcag gaaggtcccg accccatcca
    13801 gattatggga acatccaccc tgagcgctac ttacaacgat attcgtctca ccgtgggagc
    13861 gtatcccggc accgggaatc agaaccgtga tcccaggttt gtggatcccg gcaccggtaa
    13921 cctcaatctg ggcatcagaa gccgctgcat tgatgccggg gaccctggtt ggcctctcga
    13981 tcccgatggt tccagagcgg acctgggagc gctgtatttc gaccagacca acatgagcta
    14041 tgagactgat ttctattgtg atatccaggc cggtccgcat cctctggctg tgcagttcta
    14101 cgacgattcc gctttcagtg ccagctcctg gaactgggat ttcaacggtg acggcaccat
    14161 agacagcact cttcgtgatc ccctctggac tttcgaacaa cctggcagct tcagcgtgag
    14221 cctcagctca tctgatggaa gcaacactgc aactgtcact tacgatgatc tgatcagcgt
    14281 ctggaacacc gccccggaag tgctgcagcc cattgaagcg caaaactggc cggaggattt
    14341 tggtctctgg gtttgccagc tggacgcttt cttcaatgac gcagatggag actctctttc
    14401 ctactcagtg gctctgagcg aaccccttgt gagctgggaa attacgggtt ccgacctcag
    14461 aatccacagc atccaggacc tctttggggc cttgactgtt accgtgactg ccgacgatgg
    14521 ctactcggag gcaagagcca aaaatggctt gagggtcgag tgtagcttga gcttcaatgt
    14581 gactatcgat ccagtgaacg acccgcccga gctcatatca gccttcccgg agcagtcgtt
    14641 gctctacatt gccgagggag atgagatcga tttcaacctc gaagtggtgg atgtggattc
    14701 ccctgtggct tatatctggc tgctggacaa cgtggaccag aataacaacg gcccagagtg
    14761 gagccatgtg tttgacgagc agggttacca ctctgtatct gtaactatat cagatggagt
    14821 aaattccttc ttgtggggat gggggctgca ggtgcaaagc tccccatcgg acgatcctct
    14881 ggtgccggct ttcacggccc tgttgccaaa tacccccaat ccctttaggg aaggcacttc
    14941 aatccattat gccatcaatc ggcaaggtca gtttgagatg gctatttaca acagccgggg
    15001 ccaattggtg aaacgctttc agaattgccc tggggaggcc ggttggcatc atcaatactg
    15061 gaatggctgt gacagccacg gcaggaaagt cagcgctgga gtttaccttg tcagaatgac
    15121 ccacgagggt aaaagctaca cccgcaaagt gctattggtc agataggcat aacttataga
    15181 aggatatatc gatagccatt ctggatttat gcctggaaag gtgctggaaa gccgcttgtg
    15241 tgatgattaa tagatgagca ggagaattat ctcttgacat ccagataacg gctgttagac
    15301 tagctccaca ctgaagcata ccgcttcagg gattagatag tgatctgttc cataaggagg
    15361 aaagatgaca aagtcgagtc tcttttcgag cgtgagggct atcctcacgg ttcttgtagt
    15421 aatattgctt gtaagtgctt gtgacgacga caatcccacc aatcccaccg tcaacacggg
    15481 agtggtgtcc ggaacagtct ttaaggctgc cagaacggtt ttacctggtg tcacggtcag
    15541 tatcggaact cagaccaccg agaccgatgc ggacggcaag ttctttatct atggcgtcaa
    15601 tgccggaacg tcggtaattg tggatttcga gaaggaaggc tatattgcca accagaaagt
    15661 gattacggtc aataaagata aaaccaccta catcgacagc actctcagga ttccattggc
    15721 caccactttt gcctcaacgg taggcactat gctcgtggat ggatatactg agattacaat
    15781 ccctgaaaat gcttttatgg tgggctcgac tcctttcact ggaaacgtga gagccgagta
    15841 tcgatattat gaccccacca acgcggacaa cctaaacgcc tttccaggtt cattttctgg
    15901 agtgcagaca gacggcaccg agacgatgtt tgaaagctat ggctacatct ccgccagctt
    15961 cacagatgct caggacccca acaggcagtt gcaactggca gccggaaaaa ccgcctcgat
    16021 tctgtcctac attcccacca gccttttggc caacgcgcct gccaccatcc cgatgtggta
    16081 ttacaacgaa agcacgggta aatggatgga agacggtgtg gccaccaagg tagggaatta
    16141 ttaccagggg gaagtgactc actttagcta ctggaacttt gatcacccca tcgtggtgga
    16201 cgatcaatcc actctcaccg gcagagtagt ctctgcagag agcaaagccc ctattctcgg
    16261 agctcaggta gtagccacag gggtcgacta tgccggatat acaagagtct atagcgatgc
    16321 caacggtctc tttagcatca ccgtcaaggc ttctgcccag gtcacgatca aagcatattc
    16381 aggcatcaac tcctcaccag tttcacctgt catcaacacc cctgtcagcg gtggaacaca
    16441 ggacgtgggt gatatcagca tacctgatct cagcttcacg ttaaccggca agctggttga
    16501 cacatccaac cagcctattt cagctggtta cggacaaatg actcaggtta atccccctga
    16561 tggtgtaaac ccattcaatg catggattgg acttgatgcc aatggcaatt tctcaattca
    16621 ggaagcctac agcggcagct tgaccagctt tccggtgcag ttcagcatca accagcgcgg
    16681 gaacctgtat tctagcagta tcactttcac agttcctcaa cctggtcagg tttacaactt
    16741 cggtaccatc gtcatgagag agggtggtaa actgaatggc cgggccaagg atcacctggg
    16801 caactggatc gcagactcct gggtatcctt tatgcaggaa ggtgccactg gcgagggtaa
    16861 ccatgtttcg ggaagtacgg acgccaacgg ttacttcaca cttacaggtc cacccagcac
    16921 aaatctcacc aatatgagag gcagtgtcta tgtgaaccag accaacctcg tttccccatt
    16981 gatgagcctc aatttccctg catcgggaca ggagggaaat atcggcacgg tcattttctc
    17041 tccccagagc aaacgctgag tttcagctaa ggaataaaac aagcccgtca cttctgacgg
    17101 gcttgtttgt tacaaggtga aagtggcgta gatcagatat acttgttgat cttgccttcc
    17161 accaattctt tgatctggtg catacgctct tcggcgatgg cctcatagcg ggtcaccagt
    17221 acgggtgtcg tgttggaggc acgcacgagg ccccaaccgt ccgcgaatgt gatgcgggcg
    17281 ccatcgatat cggtgacgtc atatccttcc cgcttgaatt catcgcagac cgctgccacc
    17341 acctcaaact tgcggtcatc ggcgcatttg acgtggattt caggcgtgtt gtacatcttg
    17401 ggctggtcgg caagataaga ggagacgggc gcgctggttt ttgatacgat ctcgataaag
    17461 cgacagctca catagatggc gtcgtcaaaa cccagatagc ggtccgctaa aaacacatga
    17521 ccgctcattt caccggcaaa ttccaccccg atctctttca ttttcatctt gatgttggcg
    17581 tggccggtct tgtacatcat cggcaccccg cctcgggcac ggatatcgtc gtagaggttt
    17641 ttagagcatt tgacatcggc gatgatggtt ttaccggggt tgtcaatcag ataatcgcgg
    17701 gccaggatat tgaggatctg atcgccaaac agcattttgc ccttttcatc caccaccccg
    17761 atgcgatcgg cgtcgccgtc gaggccgagg ccaagctcat attccgccgc tacgacagcc
    17821 cggctgaggt ccaccatata cttctcgatg gtaggatcgg gatggtgatt tgggaaggtg
    17881 ccgtccggct cgcaatacat ctcgatcact tcacagccca ggcgccgtaa aatctccggc
    17941 agatagggac cgcccatacc gttgccgcca tccacgatca ctttgacggg gcgttcaagc
    18001 ttgatattgg cggtaatata gtctatatag tcggcgttga ggctatcgtc ctgcaccaga
    18061 ttgcccgccc cggtctggaa tttgccgctc tggataatac ccagcacttt ctgcagttct
    18121 tcggcataga cggaacccaa gcccagattg agcttgcagc cattgtattg ggaaggattg
    18181 tgactggcgg tcaccatagc gccgccatcc atcttcagct tccaaatcga gtaatagagc
    18241 acgggagtgg ccaccatgcc gatatccgtg acatcgcaac cggtttccag cagaccgcgg
    18301 attagctctt tcatcaatcc gggtgagctg ggacgcgcgt ccccgcctac cacaatggtc
    18361 ttcaagccct tgtctctcat ataggtgcca aagcctctac cgatcaggta ataggtctcg
    18421 gtgttgatct cttcatccac aatgccgcgg atgtcgtact gcctaaaaac ttgtgggttt
    18481 accatggtat cctcttttat ttattatggc gccgcaaacg tccccgtttg cgaagggggt
    18541 gattgtgtat cgatgtactt gatctcagaa agatccccta tctcaggctg agtatccttt
    18601 ccagcagagg ccggatcgcc aggatggcga gggcgcgatg ggagagagtg ttcttgtcgg
    18661 agtcgctcat ttcggcataa gtcagaccgg ttccgttcac ttcaaaaacg gcgtcgtagc
    18721 caaaacctcg ctggccgcgt tcctgggtgg tgataatccc ttgcacccgt ccttcttcgc
    18781 agccgatgat cccatcagga gctgccaggg caaccacggt cctgaaactg gcagcgcggt
    18841 ctgagacgcc tgacatctgc tgcagtgctt tgagacgatt gtcccgatac gtgcatccct
    18901 ctccagccca gcgggccgcg aacactccag gctcgccacc cagggcgtcg atgaagaggc
    18961 ctgtgtcgtc tgccaggcag agcaggtcgg ctgcccgggc ggtttcgagc gcttttttag
    19021 cggcgttgcc ttcaatggtg gcccggtctt cttccacggc gctgagctga ggaaaatcct
    19081 ccatcttgaa cagatggaag ggcagatcgc tgagcagagc gctgatctct ctgaatttgt
    19141 ctgggttgtt agtggcaatc aatagggagc gcatctacga tatcttaccc agccattttt
    19201 tgatcttgct caggacgttt ttgtccagga atccttccgc gacaccagtg attttctgga
    19261 tctgctgcag cacttcgcct tcaccgctcc agttgtctgc cagcaccttg gggtcggtat
    19321 cgttgagcac catattgagg cccatcacca ccagcaccag atcgtccacg tgtccgatga
    19381 tggggatgaa atccggaata atgtcaaagg gtagcatcat ataggccacg atgccgccca
    19441 ccatcaattt gcgtttccct ggcacccgtt tatccagcat cagcctggtg agcatgataa
    19501 aaaagtctgg cagcagaaag agatactcac tcagtttgcc acccatttta ccggtcttct
    19561 ggccggtcca gtctttggcc ttgcggcgca aatcctcata aaacttaagc ttatcggggt
    19621 ccatgtcgac cacccggtcg tcgatctttt ccttttcttc ttcaggaatc tcgcgtcctt
    19681 ccaatatgtc ttcgttcata agcacctctg aaatccatta taacgaaggt gagtttcacg
    19741 gtcaatgttt tttttcggct tgacagatta agcgagcttg tgacaatgga agcatgcgga
    19801 a
//
LOCUS       DAOSFN010000026        19676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2237_length_19676_cov_8.590337, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000026 DAOSFN010000000
VERSION     DAOSFN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 19676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19676
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2237_length_19676_cov_8.590337"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2350)
                     /locus_tag="PLO35_03050"
     CDS             complement(<1..2350)
                     /locus_tag="PLO35_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78672.1"
                     /translation="MKKSILAALLILALALGLWAGPVFSKDLLKQAKKGDPAAQFSLA
                     ETYRKGYGVKQDPKAAIKWYTKASEAGHAEAGYALGEIYLKGEGIKADLDKAENFFIR
                     AAWSGLLKAQLLLGRSYLQGGRFKGSGTQAVNMLALAANSGNEEAQLLLGTMYEKGNK
                     VNQNTSEALKWYGMAARAGNVDIQMKLGNICLKGLGIPKDVPAALGWYSLAANQGRTD
                     AQMLIADLYYEGREVSRDYPTALTWYQTAALDRNSRACYRLGEMYSHGQGVKADSIQA
                     LQWFTRSAEAGDLQAAAIIGKAHRLGLATPVNLDLALKWLRTAASTNHSESSLLLADM
                     YYNGIGTQTDIPTAAKLYELPAAQGVLKAQILLGQLYREGKQVPKDLKNAIKWYSLAA
                     QQGDLESQTALANLCLSSPPPLQDIRKAEYWFQQAALQGGAQEQFMLGWVYLNRFITP
                     PDDNQTLLWLKLAADQGHAPARKWLGNIYYNGYGVAPDQSLAAAHYLAAAQLGDVDSQ
                     YFLGMMYYYGIHLDQDTREAVKWLEAAARLDHPRALMMLSYLDDAGKYHSPLARSGPR
                     PELRGDPFITYGLNLVEQWDNEEDSGWGIRYSCNPGSRRESFTIGVRMETNDDPKDLI
                     AAAKSFREAADQGLPEAQHNLGVKYYLGSGVRQDFTEAVKWWRKASISGYRDASYNLA
                     CMVAQGKGTPSNPRLAAAIFEGLACLNHPKAAYALGYMYYYGLGLPRDLEKAYLWINK
                     STLVTDEDGHPVDFATEDAGTIAAQLSDFQLDAIDIEDRYLDL"
     gene            complement(2378..5287)
                     /locus_tag="PLO35_03055"
     CDS             complement(2378..5287)
                     /locus_tag="PLO35_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOH78673.1"
                     /translation="MTRWIQIVMLCLGLSLPLAGQSASFSQADRPERKPWSADRLAAF
                     NQDRADSLTGFDVQKYEITLSINDQSHVITGNVLATVLAEQSLSSLQYELRSLTVSSV
                     LVNGSPAAYTHTNGVITISLNIPAGQTFTTQVFYSGVPQLTSDIYHIGMIFSANTVFT
                     ISDPDAARQWWPCYDHPWDKALVDLHITMRSDWKVAANGLRDGITDHGNGSSTTHWLG
                     QHPMTTYLVCVTAGPYVEIPQTAGTLPIMNFVMQNQYNNALIDFQHLPDMIQYFSQVF
                     GPYPFEKYGQTVVSMSTYGAMEHQTMTTLGNYIITGNGIYEVVIAHELAHQWYGNAVS
                     FLTFKDVWLSEGFATYSEHLWTDKRFGWDSACAYVGSSYHQYYNNWENGAGPQTIYNP
                     SFNNYFAPPSYEKAASVLHMLRLKIGNANFFALLNQWFTAHMHGNVITSEFQALAEQI
                     SGQDLDQFFNQWIFGSGIPSLEYSIWNNDMSDSPGKIIARSTSPTATSFQIEVPFRVT
                     VSGISDSLYVEAGPDWTVNHYFQSLPPDLSVIANHNHWTLLRQTTEIRPLLTECLPSN
                     NSVLLAWQSFMDDPSLRYNIYRRVAGSQAWTLVNQTPIQGLSYSDTGAQNGVTYQYCI
                     AALDLGGWQSIYSPWMSATPESFGFNYGLLVVDETRDGNGSNIGPDDAMVDSFYDAAL
                     QPITYAQWDVATQGMPPLNILGNYQLLLWHADDFSHNLLVDDTQTLSGYILGGGKVII
                     SGWKTTAALNDEFFQRFAGGITPVYDNTAAFIGATSNSYPCITVDADKMMPAWNGMLP
                     YMYTFQNPANSIYTANMTPTALGNGLSAMFRADQPVNGGVLVMSGFPLYFMHAAPVRS
                     FLQMIIPQIAPNVASADSESPALSASMRAYPNPFNPSTTIEFFLPKAGRAQLSLYNVK
                     GQLVKRLLDQEQPRGAQQIVFDGVTEEGTPLPSGTYILRLNCGPVGLTRLITLMK"
     gene            5492..7738
                     /gene="bamA"
                     /locus_tag="PLO35_03060"
     CDS             5492..7738
                     /gene="bamA"
                     /locus_tag="PLO35_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOH78674.1"
                     /translation="MSIKHILLCLMLLAATVGLSAYGETIYAIKVEGQKSISPELVTS
                     AIGINVGDALDPEQVAQAIKTLYKMGVFKDIQFHSEVYRTGVNLIIRVVENPVLSHIE
                     FLGLKVVKKDRVDELLNLRVGSFWNEYQKVQVQQKLVKEYQSKGFSNARVTVFENIGE
                     DNRVRVKVEVEEGKRVAIAKISFTGNANIESKALQKRMKTKPASLLRSGRFEQEKFDT
                     DLGNLASYYKQNGYVDVGIGPWELKTINERELELLINIKEGERYILGQIRVEGNTSFT
                     SEAILTQFTLKKGEPFDEAKFEKQLAKVYSMYFDEGYIYTQISHEKIKQEKNLEVVLK
                     VVEGARARIRQIQISGNRRTKEKVIRRQLEIAPGDYYRQSQVIRSQQNIYNLGFFEPD
                     IQLDYTPINNQGDIDLQIDVVDKSSGMANGGVGYNSQDKFVGQLSLSQNNLFGNNWSS
                     SLMWEFGGSTQNFEFSFTNPNLYDSDVLLGTNLYYTEKDWSSFYYKIFTRGASLRLGQ
                     YLPWIDRTRLVAGYSLYSKKYRVTNRDKILEDPLANATLIELDSLSWRYTSSLNATVT
                     RDTRDNIFFPTRGSQVTLFSEIAGGLLGGDFDYFKQIMQVNWYAEAWEKLTLRTKWRF
                     GYVSPYGKSDDAPPDEKFYLGGTGVDGIRGFPDRSIGPAGGGTRAIIFSTELGYPIAG
                     DQIIAVGFFDAGNSFNKMRDFNFLELKKGIGAGIRIRSPFGLIGFDYAHNLNDGGWEP
                     HLQFGTTF"
     gene            7739..8686
                     /locus_tag="PLO35_03065"
     CDS             7739..8686
                     /locus_tag="PLO35_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH78675.1"
                     /translation="MIPYRHLFGPVNSRRLGLSLGVDLVPYKYCPLNCVYCEVQNTTH
                     LITERQEFFSSQEILNELDLFLKNRPELDYITFSGAGEPTLHSGIGKIVKYIKSTYPN
                     YRLALLTNGVLLNDPNVRAEIIPCDLVLPSLDAATQTGYEIINRPKPGLKVQDLIAGL
                     VEFRRAYAGQMWLEVFIIAGINDSETELEALIEAISQIAPDRVQLNALDRPGAEDWVQ
                     TAPLDLLQGILKLFAERLSMPVEIIAKAAVHLNTPSSIDLIRQIEATLRRRPCTAEDL
                     AVTLGTHINEISKVLRELSYDGKVEAKREARGVFYLWKP"
     gene            8674..9372
                     /gene="ispD"
                     /locus_tag="PLO35_03070"
     CDS             8674..9372
                     /gene="ispD"
                     /locus_tag="PLO35_03070"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOH78676.1"
                     /translation="METMNTAIVTAAGSGLRLNSAVKKQFLELAGIPILIRTLERFFA
                     SPLISSLIITAPEADQAYTEELIRQYFEAETKPWIVIAGGAERQDSVFAALQHCPPGT
                     KLVFIHDAVRPFITLDLIADLASLALKHKAAIPVARIKHTIKTIEGEVVGETLPRDRL
                     VQVFTPQVFDLKLIRAAYEKAYEDGFVSTDDSSLVEHLGHAVPYLFSSDLNLKITDAS
                     DLFFASQIIENNMV"
     gene            9385..12144
                     /locus_tag="PLO35_03075"
     CDS             9385..12144
                     /locus_tag="PLO35_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOH78677.1"
                     /translation="MKAETLGGCYNLALNKLAGRIRPLRVLIPVPVFGIGCGVLGLSL
                     AKVSLKMGEYASTLLKALEYRGYDSTGAAFQGEGEHVTLLKDVGAPSTLVKTLGIEKQ
                     AGQIFCGQVRWATFGFVNKVNAQPHEVKCKRHIYGAHNGNITNTRDLKVFLTKEGHTV
                     LSDNDGEMLVHSVEHYFDIELKKAGEPSDPEARKACMRRAIIGTAEKMVGSYAAVIVD
                     PVTRTSWAIKAGSSLYFGIGEADGVAFKLASSDLTAVLRFTKMLINLREGEFVEYTHE
                     YYQVYAQKDLRFKHLNQPDEVYATGAMITTQPVYSKLRAEDVELLPEHEYFMEQEIYA
                     QVESTGKLIKLFAGGSNTGKRMMELTRREGIYELLKTTIPQLVDAPDLITKRRHFDQL
                     CASEQWKNFYQLSRSRYPEIFDVAVKEDFDKKYFFSNEKNIFIDLIGPEFDLSRIALA
                     KALDSISELDSVEEFEHEVRDFLALIKNTVSNNRNAYTIACGTSFHATKVGALFFNEI
                     AGVEIIPILPGDFRGEYSNCIKNNDLIIGVSQSGETKDLIDIFNDIDASGLNVRKAVL
                     VNNMNSTLGQEKSDVAIPILCGPEIAVPATKSFMNQITLFYYLAIRTAQAILDELPNG
                     WDEATLAQKRQQLEKRKQSLHRIPDLIRETLDNTADVIDAMAAKIYMEPSLHILATKI
                     SGVAMEGALKIRETVLNHAEGREASEFKHGPNTILGKNTVFGIKHVRNFVRSFGDLID
                     SIDSESQRHSISREETKEMIKAVGTYLFTGNMPFNLSEAGSKLFKGIVKDKDFFEPLY
                     RNYPLIYVTGPDERDVNLTVSQINTHKIRGANTYVIAEEDDKLMKNASSNPNEGKYYG
                     WAYIILPKTGDSLLTCFSATAVLQLLALRMSVRKMKKLDRINMLDHGVHPDVPKNVSK
                     SITVD"
     gene            12151..12627
                     /gene="ispF"
                     /locus_tag="PLO35_03080"
     CDS             12151..12627
                     /gene="ispF"
                     /locus_tag="PLO35_03080"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOH78678.1"
                     /translation="MRIGHGYDVHRLVKGRPLILGGVHIPYEKGLLGHSDADVLIHAV
                     MDALLGALALGDIGLHFPDTDTRWMNADSRKLLSQVVSLLNKHNYQVVNLDATLICQA
                     PKLRPFIDAMRTNLASDLATDISNLSVKATTEEGLGISGAGGIAAHCVVLLEERKP"
     gene            12624..13379
                     /gene="mobB"
                     /locus_tag="PLO35_03085"
     CDS             12624..13379
                     /gene="mobB"
                     /locus_tag="PLO35_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOH78679.1"
                     /translation="MKALGIIGYHHTGKTTLATFLISRLWERGYKINSIKDIHNEAYR
                     ADTEGSNSWKHARAGASRVLAIGTRDAALVMTPQPDLRSALPLFDCDWLIVEGMKEAA
                     LPKLVCAVSEAQLEELVDDSCIGISGPIAGSISHFRGLPCFCLERDAVQLIDTVLAKT
                     FNILPQSDPDCCSACGFDCLTMAARIVQGTSKREDCVLDGKQELKLFVNDRQVSIVPF
                     VQNILRDTIRAQVGNLKDLDPDGAIRVEIKART"
     gene            13357..14001
                     /gene="thiF"
                     /locus_tag="PLO35_03090"
     CDS             13357..14001
                     /gene="thiF"
                     /locus_tag="PLO35_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOH78680.1"
                     /translation="MRSKPELEGLRRRFFAAHDPVMLKAWQNACVGIAGAGGLGSNVA
                     VSLTRAGIGKLVIADFDVLSVSNLNRQQYFMDQVGQTKVTALKDNLCRISPFTMILTH
                     ALRITPDNLDQLFGNCDILIEAFDLADQKTMLIESWQELYPRRPVIAASGLAGVGNNQ
                     SIVTTSCEGLYLIGDQSSELKPGVSPVSARVAIVANMQANLCLDLLLQIPRNLS"
     gene            13998..14276
                     /locus_tag="PLO35_03095"
     CDS             13998..14276
                     /locus_tag="PLO35_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78681.1"
                     /translation="MSTIRELHDDFPQISRIFARISQMVQRAVLQQALVPLNPLNSCS
                     VGFQSGASLNLLNHINICVISAKICAICGKYIADLEIDVTGSGASENK"
     gene            14273..14476
                     /gene="thiS"
                     /locus_tag="PLO35_03100"
     CDS             14273..14476
                     /gene="thiS"
                     /locus_tag="PLO35_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOH78682.1"
                     /translation="MSTIIVNGHTIPWEDGMTVRRVLQVMNYSFRMLVIKIDGKLVKR
                     ADYDHTLVPAGSDVMVYHMISGG"
     gene            14555..14866
                     /locus_tag="PLO35_03105"
     CDS             14555..14866
                     /locus_tag="PLO35_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78683.1"
                     /translation="MNIHFRDVQTGNVEARAILEIADGVFLNEITILNYEGQITVEFP
                     RKSFVGKNKRTFYLDILSFEDNDKRIVWELEIKSAYNEWRKSNKKILIYNKSNIDGGS
                     S"
     gene            14863..16083
                     /locus_tag="PLO35_03110"
     CDS             14863..16083
                     /locus_tag="PLO35_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOH78684.1"
                     /translation="MITDLQATLSTNIKGMKRSAIRELLKFLGQPGLISLSGGFPSPL
                     TFPTVELKEIMCEVMDTEAAFALQYGATEGDTLLRSLLVNRYKAAGVDMSIENMIITT
                     ASQQALDLIAKIFINRGDSVIVGLPSYLGGLSAFNSYGANMIGIPMDDEGEDVQIMEE
                     KIKEQIAQGNKPKFIYVIPDFQNPAGMTMSERRRKEVIDIAHRYDILILEDSPYRELR
                     YEGSHQKTIYELEGNGQVILLGTFSKIFCPGFRIGWVVGHPDILDKIVVAKQATDLCT
                     PPFTQRIAARYIEKGLLDPKIQQIREMYSVKQQGMLKSLAEFMPEEFSWTHPEGGLFL
                     MAKGPEWIDTNAMLLDCIKNANVAYVAGTSFFCDGGGQNTMRLNFSYETLDTNHEGAR
                     RLGNYYKDVIAKHK"
     gene            16179..16478
                     /locus_tag="PLO35_03115"
     CDS             16179..16478
                     /locus_tag="PLO35_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH78685.1"
                     /translation="MPKKVEKDKMGMIIREEDALEKMEVEITEVKDPGDTDSPVLIYY
                     NWCKKCGICVAFCPTGCLGRKADGSPYVASPEKCIHCETCDRLCPDFAITGAKDR"
     gene            16489..17754
                     /locus_tag="PLO35_03120"
     CDS             16489..17754
                     /locus_tag="PLO35_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOH78686.1"
                     /translation="MPSPKKTAIAKPRGKASKTVKLPKATEETPKVESPLKELEAKRE
                     RKNVLMQGNEAVAFGALDAGCNFFAGYPITPSTEIAEILALELPKRGGTFVQMEDEIA
                     SICAITGASLAGAKALTATSGPGFSLMQEGIGFAKITETPCVVVNVQRMGPSTGMPTS
                     PAQGDIMQAKWGSHGDSPAIVLYPDSVKESYELTIRAINLSEKYRTCVILLLDEVLGH
                     MRESVMLPDMANVRVISRIKPTVPPHWYKHYDENQKYLSPLASYGEGYRFHVTGLTHD
                     ALGFPTNKATEAAEMMERLRKKISYNIRDLVQIESHQMEDATIALFAAGITARSAKAA
                     VAIARSEGVKVGLLRPLTIWPFPDDAVRKMLQNVETVIVPELNQGQLINEIKRLTKNK
                     SDSNVIGLQRVNGKLITPNDILRKIKEVM"
     gene            17754..18596
                     /locus_tag="PLO35_03125"
     CDS             17754..18596
                     /locus_tag="PLO35_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH78687.1"
                     /translation="MANIPQRVIHDYLRHDKKFPHVWCAGCSNGIVLGSIIRGIASMQ
                     IDRDDIVMVSGIGCSSRMPVYVDFNTLHTLHGRSIAYATGVKLHKPHLKVIVITGDGD
                     CTAIGGNHLIHAARRNIDLSVILINNNIYGMTGGQCSPTTPHDAIATTAPYGNIEPDF
                     NICELALGAGATHVARTTAFHTQEVQKMVVEAIEHPGFSLIEVVSACPVIFGRLNKKG
                     GPAQMMKDMRDGSIPLTAVDKLPPEKVEGKIIRGTLRKDVKPEFCAAYGELQKRAQAM
                     GDVK"
     gene            18593..19135
                     /locus_tag="PLO35_03130"
     CDS             18593..19135
                     /locus_tag="PLO35_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH78688.1"
                     /translation="MKNYEIRLSGSGGQGLILAGIILARAAVIDKRKVTQTQSYGPES
                     RGGSSRADVIISDHDIYFPEAMNFNCLLALTQEACDKYLFDLREEGVLIIDTTFVKNL
                     ALAAENTYELPFTDIAIDKLGSAISTNILSLSFLVRLTKVVKESSLEQSIRESVKPAF
                     IDLNLKAMHIGFELADNYKK"
     gene            19144..19545
                     /gene="mce"
                     /locus_tag="PLO35_03135"
     CDS             19144..19545
                     /gene="mce"
                     /locus_tag="PLO35_03135"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOH78689.1"
                     /translation="MVKHISHIGIAVKDLDAGIEFYKKLGLELEAVEEVPSQMVKVAF
                     FPCGDTRIELLAPTSEESPVAKFIEKKGEGIQHIAFAVDDLPAALQESSDKGITLIDK
                     EPRPGAHHADIAFLHPKSSMGVLIELCKEKH"
BASE COUNT         4703 a         5444 c         5283 g         4246 t
ORIGIN      
        1 aaaggtccag gtatcggtct tctatgtcta tcgcgtccaa ctggaaatca gacaattggg
       61 ccgcgatcgt tccagcgtct tcagtggcga aatctaccgg gtgaccgtcc tcatctgtca
      121 cgagcgtgga tttgttgatc cataagtagg ccttttcgag gtcacgaggg aggcccagtc
      181 cgtaataata catgtagcca agcgcgtagg cagccttggg atgattcaga caggccaggc
      241 cctcaaaaat agcggctgcc agccggggat tggatggcgt gcctttaccc tgggcgacca
      301 tgcaggccag gttgtaactg gcgtcccggt aaccggatat ggaagctttg cgccaccatt
      361 tgaccgcttc cgtgaaatcc tgtctgactc cactgcccag gtagtatttg actcccaggt
      421 tgtgctgagc ttcagggagg ccttggtcgg cggcctcacg aaagcttttc gcggccgcga
      481 tgaggtcctt ggggtcatcg ttcgtctcca tcctgacacc gatggtgaag ctttctcttc
      541 gggagccggg attgcaggaa tagcggatac cccagccgga atcctcctca ttgtcccact
      601 gctccacgag attgaggcca taagttatga agggatcgcc acgtagttca gggcgcgggc
      661 ctgatctggc gagggggctg tgatatttgc cggcatcatc taaatagctc agcatcatta
      721 gcgcgcgggg atgatccagt cgagcggcgg cttccagcca tttgaccgcc tcgcgggtgt
      781 cctgatcgag gtgaatgcca tagtagtaca tcatccccag aaaatattgg gaatcgacgt
      841 ctccgagctg ggcggcggcc agatagtgag ctgccgccag cgattgatca ggggccactc
      901 catagccgtt gtaataaata tttcccagcc atttgcgagc aggagcgtgt ccctggtcgg
      961 cggcaagttt gagccagagc aaggtctgat tgtcgtccgg gggagtgata aaacggttca
     1021 gatacaccca gcccagcata aattgctcct gagctccacc ttggagggcg gcctgctgaa
     1081 accagtattc ggctttcctg atgtcctgga gagggggggg actgctcagg cagagatttg
     1141 ccagggccgt ttgggattcc agatcaccct gctgagcggc caggctatac cattttatgg
     1201 cgtttttaag gtctttgggc acttgcttgc cttcccggta aagctgtccc aggaggattt
     1261 gagcttttaa aacaccctgg gcagcgggta gttcatacag cttggctgcg gttggaatat
     1321 cggtctgggt gccgatcccg ttgtaataca tatctgccag caggagagac gattcgctgt
     1381 ggttagtgct ggctgccgtc cgcagccatt tgagtgccag atcgaggttc acgggggtag
     1441 cgaggcccag tcgatgagct ttgccgatga tggccgcagc ctggaggtct cccgcttcag
     1501 cggatctggt aaaccactgc agcgcttgga tggaatcggc tttcaccccc tgtccgtgac
     1561 tatacatttc tcccaggcgg tagcaagccc gggaatttct gtccagggca gcggtctgat
     1621 accacgtcag ggctgtggga tagtcccggg atacctccct gccttcataa tagaggtcgg
     1681 caatcagcat ttgagcgtct gtcctgccct gattggccgc gagagaatac catcccagcg
     1741 ccgcggggac gtctttggga atacccagcc ctttcagaca gatattgccc aacttcatct
     1801 ggatgtccac attgccggcc cgggcggcca tgccatacca tttcagggct tcggaggtgt
     1861 tttggttcac cttgttgcct ttctcataca tggtccccag tagcagctgg gcttcttcat
     1921 taccggaatt ggcagccagg gccagcatgt ttacggcttg ggtcccggac cccttgaagc
     1981 ggcctccctg caggtatgat ctgccaagca gcagctgagc cttgagcaat ccggaccagg
     2041 cagcgcggat aaaaaagttc tcggctttat caaggtcggc tttgattcct tcccctttca
     2101 gatagatttc tcccagagcg tagcctgctt ctgcatggcc agcctcggag gctttggtgt
     2161 accacttgat ggcggctttg ggatcttgct tcacgccata tcccttccga taggtctcag
     2221 ccagagagaa ctgcgcggcc ggatcacctt ttttggcctg tttgagcagg tctttgctaa
     2281 agacggggcc agcccacagc cccaacgcca gcgctaaaat taacagggcg gccaagatgg
     2341 attttttcat attgcctccc tcccggggga tatatgctta tttcatgaga gtgatcagac
     2401 gggtaaggcc cacggggccg caattcaacc tgagtatata ggtccccgag ggcagcggag
     2461 tgccttcctc cgtcacgccg tcaaagacga tttgctgcgc tccgcggggc tgttcctgat
     2521 ccagtaaacg cttgaccagc tgtcctttga cgttgtaaag gctgagctga gcgcgaccgg
     2581 ctttcggaag gaaaaactca atggtggtgc tgggattgaa gggattgggg taagctctca
     2641 tgctggcaga aagcgctggg ctttcagaat ccgcagaagc cacgttgggc gcgatctgag
     2701 gtatgatcat ctggagaaac gatctcactg gggcagcatg catgaagtaa agcggaaaac
     2761 cactcatcac caggactcca ccgttgaccg gttggtctgc tctgaacata gcgctcaggc
     2821 cgtttcccag ggcggtggga gtcatattgg cggtgtagat ggagttggcg gggttctgga
     2881 aagtatacat gtagggcagc atcccgttcc aggcgggcat catcttgtca gcgtccaccg
     2941 tgatgcaggg atagctgttg ctggtagcgc cgatgaaagc ggcggtgttg tcatagactg
     3001 gtgtgattcc acctgcaaag cgttggaaaa attcatcgtt gagggcggca gtggtcttcc
     3061 atccggaaat gatcaccttg cctcccccca ggatgtaacc gctcagagtt tgagtgtcgt
     3121 ccaccagcag gttgtgagaa aagtcatcgg cgtgccagag cagcagctgg tagtttccca
     3181 gtatattaag aggcggcatg ccctgagtgg ccacgtccca ctgggcatag gtgatgggct
     3241 gcaaggccgc gtcgtagaag ctgtccacca tggcgtcgtc cgggccgatg ttgctaccgt
     3301 ttccatctct ggtttcgtct accaccagca gtccgtagtt gaagccaaag ctctccggcg
     3361 tggcgctcat ccaaggtgaa taaatactct gccagccacc cagatccagc gcggcaatgc
     3421 agtactggta ggttacgccg ttctgagccc cagtgtcgga atagctgagc ccttggatgg
     3481 gagtctggtt cacgagtgtc caggcttggc ttccagccac tctgcgataa atattgtatc
     3541 tgaggctggg gtcgtccata aagctctgcc atgccagcag cacggagttg ttggaaggca
     3601 ggcattccgt gagcaggggc cgaatctctg tggtctggcg cagaagggtc cagtggttgt
     3661 ggttggcgat cacgctcagg tccggaggca agctctggaa atagtggttg acggtccagt
     3721 ccggcccggc ttccacataa agggaatcgc tgatcccgct cactgtgacc cggaagggga
     3781 cttcgatctg aaagctggtg gcggtgggag aagtggagcg ggctatgatc ttgccggggc
     3841 tgtcgctcat gtcgttgttc cagatgctgt attcgaggct ggggatgccg gagccgaaga
     3901 tccactggtt aaaaaactga tccaggtcct ggccgctgat ctgctccgcc agcgcttgaa
     3961 actcactggt gatcacgttt ccatgcatgt gggcggtgaa ccactgattg aggagggcaa
     4021 agaagttggc gttgccgatc ttcagcctca gcatgtggag cacggaggcg gccttttcat
     4081 aggaaggagg agcgaagtag ttgttgaagc tggggttata gatagtctga ggcccggcgc
     4141 cgttctccca gttgttgtag tattgatgat aactggagcc cacgtaggcg caggcggaat
     4201 cccatccgaa gcgtttgtcc gtccataaat gctcggagta ggtggcaaag ccttccgaga
     4261 gccagacatc cttgaaagtg aggaagctga cggcgttgcc gtaccactga tgggcaagtt
     4321 cgtgagcgat caccacttca tagatgccgt tgccggtgat gatgtagttg cccagagtag
     4381 tcatggtctg atgttccata gcgccgtagg tgctcatgct gaccacggtc tggccatatt
     4441 tttcaaaggg ataggggcca aagacctgag agaaatactg gatcatgtct ggcagatgtt
     4501 gaaaatcaat cagggcgttg ttatactggt tttgcatcac gaagttcatg atgggcagcg
     4561 tcccggcggt ctgtggaatt tccacatagg gccccgcagt tacgcagacc agataggtgg
     4621 tcatgggatg ctggcccagc cagtgagtgg tgctgctgcc gttgccgtgg tctgtgatgc
     4681 cgtcccgcag accgttggcg gccactttcc aatcggagcg catggtgata tgcaagtcaa
     4741 ccagagcttt gtcccagggg tgatcataac agggccacca ttgcctggca gcgtcggggt
     4801 ctgagatagt gaagacggta ttggcgctga agatcatgcc gatatgatag atatccgagg
     4861 tcagctgcgg aacgcctgaa tagaaaactt gagtggtgaa ggtctggcca gcggggatgt
     4921 tgaggctgat ggtgataact ccgtttgtat gggtataggc ggccggactg ccgttcacca
     4981 gcacgctgga aaccgtaagg cttctgagct catactggag agaactgagg ctttgttccg
     5041 ccagcacggt ggccaggacg ttccccgtga tcacatggct ctgatcgttg atcgacagcg
     5101 tgatctcgta tttctggacg tcaaatccgg tcagagaatc ggcacggtcc tgattgaagg
     5161 ctgccagtct gtccgcgctc cagggcttgc gctccggtct gtccgcttgc gaaaaactag
     5221 cgctctggcc tgcaagcggc aagctcagtc ccaggcacaa catcactatt tgaatccatc
     5281 tggtcatcat cacctcacgt ggggatgccc tcactcctcc atccgttggg aagcacagtt
     5341 ccgggctgga atatcaggca tccattatct tcattgagtt atagcaagtt tgtttccaga
     5401 ggcgggataa agtcaagcga aaaaaaactc tttacgaaaa cggggcttcc aaagaacttg
     5461 tctgacagct ctattacgag gtaaaaaaca catgtcgatc aagcatattc tgctgtgcct
     5521 tatgctgctg gccgctaccg ttggcctgtc cgcgtatggc gagaccatat acgcgattaa
     5581 ggtggaaggc caaaaaagca tttcacccga actggtgact tccgcgatcg gaatcaacgt
     5641 gggcgatgcc ctcgatcccg aacaggtggc ccaagccatc aaaaccctat ataaaatggg
     5701 tgttttcaaa gacatccagt ttcattccga ggtgtatcgc accggagtga acctcatcat
     5761 cagagtggtg gaaaaccccg tgctttccca catcgagttt ttggggctca aagtggtcaa
     5821 aaaagaccgg gtggacgagc ttttaaacct gagagtcggc tccttctgga atgagtatca
     5881 gaaggtgcag gtccagcaaa aactggtgaa agagtatcag agcaaaggct ttagcaacgc
     5941 ccgggtgacg gtgtttgaaa acatcggaga agacaaccgc gtgcgggtca aagtggaagt
     6001 ggaagagggt aagcgggtgg cgatcgccaa gatcagcttc accggtaacg ccaatatcga
     6061 atccaaagct cttcagaaac gcatgaagac gaagccggcc tcgctgttgc gctccggacg
     6121 ctttgagcag gagaagtttg acaccgacct tggcaatctg gcatcatact acaagcaaaa
     6181 cggttatgtg gatgtcggga tcggcccctg ggagttgaaa accatcaacg agcgggagct
     6241 tgagttgctg atcaacatca aggaaggaga gcgctacatc ctgggacaga tcagggttga
     6301 aggcaacacc agctttacct ccgaagccat tctgacccag ttcacgctca aaaaaggcga
     6361 gccctttgac gaagccaagt ttgaaaaaca gctggccaaa gtctattcca tgtactttga
     6421 cgagggctat atctacaccc agatcagcca tgaaaagatc aaacaggaga agaatctgga
     6481 ggtggtgctc aaggtcgttg aaggcgccag ggccagaatc cgtcagatcc agatttccgg
     6541 aaaccggcgc accaaagaaa aggtgatcag acgccagctg gagatcgctc ccggagatta
     6601 ttaccgtcag agccaggtga tccgctccca gcaaaacatc tacaatctgg gcttcttcga
     6661 gccggacatc cagctggact acactcccat caacaaccag ggcgatatcg acctccagat
     6721 cgacgtggtg gataaatcct ccggtatggc caacggtggt gtgggctaca acagccagga
     6781 caaatttgtg ggccagctct cgctttctca aaacaacctc ttcggcaaca attggtccag
     6841 cagcctgatg tgggaatttg gcggcagcac tcagaacttt gaattcagct ttaccaatcc
     6901 caatctctac gattccgatg tgttgctggg caccaacctc tattataccg aaaaagactg
     6961 gagctctttc tactacaaga ttttcacccg tggcgccagc ctcaggctgg gccaatatct
     7021 gccctggata gaccgcaccc gtctggtggc cggatattca ctttactcca aaaaataccg
     7081 cgtcaccaac cgcgacaaga tcctggaaga tccgcttgcc aatgccactt tgatcgaact
     7141 ggattctctc agctggcgct acaccagctc cctgaacgcc accgtgactc gcgacacgcg
     7201 cgacaatatc tttttcccca cccggggctc tcaggtgact ctattttctg agatcgccgg
     7261 tggactgctg ggcggagact ttgactattt caagcagatc atgcaggtca actggtatgc
     7321 cgaggcctgg gaaaagctca cgttgcgcac caagtggcgc tttggctatg tgagccccta
     7381 cggtaaaagc gatgacgctc ctcccgacga gaagttttat ctgggcggaa ccggagtgga
     7441 cggcattcgc ggctttccag accgttctat aggacccgcc ggaggtggca cccgtgccat
     7501 catcttctcc acagaactgg gctatccgat cgccggtgat cagatcatcg cggtgggatt
     7561 ctttgatgcc ggcaacagct tcaacaagat gcgggatttc aacttcctgg agctcaagaa
     7621 aggcatcgga gcaggcatcc gcattcgcag tccctttggc ctcattggtt ttgattacgc
     7681 ccacaacctc aacgacggcg gctgggaacc tcatctgcag ttcggcacta ccttctaaat
     7741 gattccctac cgtcatctgt ttggacctgt caattccagg cggcttggcc tttccctggg
     7801 cgtcgatctg gtcccgtata aatactgccc cttgaactgc gtttattgcg aagtgcagaa
     7861 taccactcat ctgatcacgg agcgccagga gttctttagc tcccaagaga ttctcaatga
     7921 gctcgatctc tttctcaaaa accgtccgga gctggattac atcaccttct ccggggcggg
     7981 agaacccact ctgcacagcg ggatcggtaa gatcgtcaaa tacatcaaat ccacctatcc
     8041 aaactaccgg ctggcgctgc tcaccaacgg agtgctgctc aatgatccta atgtgcgggc
     8101 ggagattata ccctgcgatc tggtgctgcc gtctttggat gccgccaccc agacgggcta
     8161 tgagatcatc aaccgtccca aaccaggcct gaaggttcag gacctcatcg ccggattggt
     8221 ggaattcaga cgggcatacg ctggccagat gtggttggaa gtatttatca ttgctggaat
     8281 caacgatagc gaaacagaac tggaagccct catcgaagcg atctcacaga ttgccccgga
     8341 ccgtgttcag cttaacgctc tggaccgtcc cggagcggaa gattgggtgc agaccgcccc
     8401 gctggatctg ctgcaaggga ttttgaagct gtttgcggag cgcttgtcca tgccggttga
     8461 gatcatcgcc aaagcggcgg tccatctgaa cactcccagt tccatcgatc tgatccggca
     8521 aatcgaggct acgctgcggc gccgtccctg caccgctgag gatctggctg tcactctcgg
     8581 cacccatatc aacgagatca gcaaggtgtt gagggaactt agctatgacg gtaaagtgga
     8641 ggccaaacgc gaggccagag gagtcttcta tctatggaaa ccatgaacac ggcaatagtt
     8701 acagcggctg gtagcggttt gaggttgaac tctgccgtca aaaaacagtt tttggaactg
     8761 gctgggatcc ccatcctgat ccgcaccctg gagcgcttct ttgcttcccc gctgatttcc
     8821 agcctcatca tcacggcccc cgaagccgat caagcctaca cggaagagct tatccggcaa
     8881 tattttgagg cagagaccaa accctggatt gtgatcgctg gcggagccga acggcaggat
     8941 tccgtatttg ccgccctgca gcattgccct ccgggcacca aactggtctt tatccacgat
     9001 gccgtgaggc ctttcatcac tctggacctg atcgcggacc tggccagtct ggcgctgaaa
     9061 cataaagccg ccatcccagt tgccaggatc aagcacacca tcaagaccat agaaggcgaa
     9121 gtagtggggg agaccctgcc gcgggaccgt ctggtgcagg ttttcacacc tcaggtcttc
     9181 gatctgaagc tcatccgggc cgcctatgaa aaggcctatg aggatggatt tgtgagcacg
     9241 gacgattcct ccctggtgga gcatctgggg cacgcggtgc cttatctctt cagctccgat
     9301 ctcaatctca agatcacaga cgcctcagac ctcttcttcg cctctcaaat cattgaaaac
     9361 aatatggtat aaaaggatat ttacatgaaa gcggaaaccc ttggcggttg ttacaatctg
     9421 gccctgaaca agttagccgg tcggatcagg cctctccgcg tcctgattcc agttccggtc
     9481 ttcggtattg gatgtggagt cctgggccta tctctggcaa aagtatccct taagatggga
     9541 gaatacgctt caacactctt aaaagctttg gaatacaggg gttacgactc cactggggct
     9601 gcctttcaag gcgagggtga gcatgtaacc ctgctcaaag atgtgggagc tcccagcacc
     9661 ctggtcaaga ccctgggcat cgagaaacaa gctggacaga tcttttgcgg ccaggtcaga
     9721 tgggccacct ttggttttgt caacaaagta aacgcccagc cgcatgaggt gaaatgcaaa
     9781 cgccatatct acggcgctca caacggcaat atcaccaaca cccgtgatct caaggtcttc
     9841 ctcaccaaag agggccacac cgtcctcagc gacaacgatg gtgaaatgct ggtgcacagc
     9901 gtggagcact atttcgatat cgagctgaaa aaagccggcg aacccagcga tcccgaagcc
     9961 cggaaagcct gcatgcgcag agccatcatc gggaccgccg aaaagatggt gggttcctac
    10021 gcggcggtga tcgtggatcc cgtcacccgc acttcctggg ccatcaaagc tggcagcagt
    10081 ctctattttg gcatcggtga ggcggatggc gttgctttca agctggcctc ctcagatctc
    10141 acagccgtgc tgcgctttac caagatgctg atcaatctgc gggaaggcga atttgtggaa
    10201 tacactcacg agtattacca ggtttacgca cagaaagatc tgcgcttcaa acacttgaac
    10261 caacccgatg aagtctatgc caccggcgcg atgatcacca cccagccggt ctattcaaaa
    10321 ctgagggctg aggacgtgga actgctgccg gagcatgaat actttatgga gcaggagatc
    10381 tacgcccagg tggaatccac cggcaagctg atcaagctct tcgcgggagg ctcaaacacc
    10441 ggcaaacgca tgatggaact cactcgcaga gaaggtattt acgagctttt gaaaaccacc
    10501 attccccagc tggtggacgc tccggacctg atcaccaaac gccgccactt tgatcagctc
    10561 tgcgcttccg aacagtggaa aaacttctac cagctttccc gcagccgcta tcccgaaatc
    10621 tttgacgtgg cggtcaagga agactttgac aagaaatact tcttttccaa tgaaaagaat
    10681 atctttatag acctgatcgg tcctgagttt gacctcagcc gcatcgctct cgccaaggcc
    10741 ctggacagca tctcagagct ggacagtgtg gaggaattcg agcatgaggt gcgggatttt
    10801 ctggccctga tcaaaaacac cgtcagcaac aaccgcaacg cctacaccat cgcctgcggg
    10861 accagctttc acgccaccaa agtgggtgcc ctcttcttca acgagatcgc cggggtggag
    10921 atcatcccca tcctgccggg tgatttcaga ggagaatact ccaactgcat caaaaacaac
    10981 gacctcatca tcggggtctc ccaatccggc gaaaccaaag acctcatcga tatcttcaac
    11041 gacatcgacg cttccggcct gaacgtccgt aaagctgtgc tggtcaacaa tatgaactct
    11101 accctcggcc aggaaaagag cgacgtcgcc atccccattc tctgtggacc ggagatcgct
    11161 gttcccgcca ccaagagttt catgaaccag atcaccctct tctattatct ggccatccgc
    11221 accgcgcagg ctatcctcga cgagctcccc aacggttggg acgaggctac tttagcccaa
    11281 aaaagacagc agctcgagaa gcgcaaacag agcctccacc gcatcccaga cctgatcaga
    11341 gagaccctgg acaacaccgc ggacgtgatc gacgccatgg ccgccaaaat ctatatggag
    11401 ccgtctctgc atatcctggc caccaagatc agcggagtgg ccatggaagg cgccctcaag
    11461 atcagagaga ccgtgctcaa ccatgccgaa ggccgcgaag ccagcgagtt taaacacggt
    11521 cccaacacca tcctgggcaa gaacaccgtc tttggtatca agcatgtgcg caactttgtg
    11581 aggtccttcg gcgatctgat cgacagcatc gacagcgagt cccagcgcca ctccatcagc
    11641 cgtgaagaga ccaaggaaat gatcaaagcc gtgggcacct acctctttac cggcaacatg
    11701 ccattcaacc tcagcgaagc gggcagcaag ctctttaaag gcatcgtgaa ggacaaggat
    11761 ttctttgagc ccctctatcg caattatcct ctcatctacg tcaccggacc ggacgaacgc
    11821 gacgtcaatc tcaccgtctc gcagatcaat acccacaaga tccgcggcgc caatacctat
    11881 gtgattgccg aggaagacga taagctgatg aaaaacgcca gctcaaatcc caacgaaggc
    11941 aaatactacg gatgggctta tatcatcctg cccaaaaccg gtgacagcct gctcacctgt
    12001 ttttcggcta cagctgtgtt gcagctcctg gccctcagaa tgagcgtccg caagatgaaa
    12061 aagctggacc gcatcaacat gctggaccat ggtgtccatc ccgacgttcc caaaaacgtc
    12121 tccaaatcga tcacggtgga ttgagcggtg atgagaatcg gccacggcta cgacgtccac
    12181 agattggtga aaggcaggcc tctcatcctg ggaggagtcc atatccctta tgagaagggg
    12241 cttttaggcc actcggacgc ggatgtattg atccacgccg tcatggatgc cttgctggga
    12301 gcattggcat tgggagacat aggcttacac tttccggata ctgataccag gtggatgaat
    12361 gccgacagcc gaaaactgct gagccaggtt gtctccctgc ttaacaaaca taattatcaa
    12421 gtagttaacc tggacgccac tctcatctgc caggccccca aactgcgtcc ctttattgac
    12481 gccatgagga ccaatctggc ctccgacctc gctaccgaca tttccaatct atccgtcaaa
    12541 gccactaccg aagaaggtct tggcatcagt ggcgcgggag gtatagcggc acattgtgta
    12601 gtcctgctcg aggagcggaa gccatgaaag ctttaggaat catcggctat catcacaccg
    12661 gcaagaccac tctggccacc ttcctcatct ccaggctctg ggaacgcgga tataagatca
    12721 actccatcaa agacatccac aacgaggcct acagagcgga cacggaaggt tccaacagct
    12781 ggaaacatgc acgtgcaggc gcttccagag tgctggctat cggcacccgg gacgccgccc
    12841 tggtgatgac ccctcagccc gatctgagat ccgccctgcc gctgtttgac tgcgactggc
    12901 tgatcgtgga aggcatgaag gaagcggcct tgcccaagct cgtctgcgcg gtcagcgaag
    12961 cgcagcttga agagctcgtg gatgacagct gcatcggcat tagcggcccc atcgccggat
    13021 ccatcagcca ttttaggggc ttgccttgct tctgcctgga gcgggacgcg gttcagctaa
    13081 tagacacggt attggcaaag acctttaata tcctgcctca aagtgacccg gattgctgtt
    13141 ccgcctgcgg atttgactgc ctgactatgg ccgcccggat cgtgcagggc acctcaaagc
    13201 gtgaggactg cgtgctggat ggaaagcagg agctaaagct gtttgtgaat gaccggcagg
    13261 tctcgatcgt tcccttcgtt caaaacatcc tccgcgacac catcagagcg caggtgggca
    13321 acctcaaaga cctcgatccc gatggagcga tccgcgttga gatcaaagcc agaacttgag
    13381 gggttgagga ggcgtttctt cgccgctcac gatcccgtca tgctgaaagc ctggcaaaac
    13441 gcctgcgtgg gaattgccgg agcgggaggt ctgggctcaa atgtggcggt ttcactcacc
    13501 cgggccggca tcggcaaact ggtgatcgct gattttgacg tgctcagcgt gtccaatctc
    13561 aatcgccagc aatactttat ggaccaggtg gggcagacca aggtgaccgc cctcaaggat
    13621 aatctctgcc gcatttcacc tttcaccatg atcctcaccc acgcgctcag aatcacgccg
    13681 gataatctgg atcagctctt tggaaactgc gacatcctca tcgaggcctt cgacctggca
    13741 gaccagaaaa ccatgctgat cgaatcctgg caggagctct atccccggcg gcctgtcatc
    13801 gccgcatccg gacttgccgg agtgggcaac aatcaatcta tcgtaaccac aagctgtgaa
    13861 ggactatacc tgatcggaga ccagtcctcg gagttgaaac cgggcgtcag cccagtttcc
    13921 gcgcgagtag ccattgtggc aaatatgcaa gccaatctat gcctggattt gctcttacag
    13981 ataccaagga acctttcatg agcacgatca gagagctgca cgacgatttc ccgcagattt
    14041 cgcggatttt tgcgcggatt tcgcagatgg tacagagagc ggtgctacaa caagcactgg
    14101 tacctctcaa ccctctcaac tcatgtagcg tcggattcca atccggggcg tctctaaacc
    14161 ttctcaacca tatcaatatc tgcgtaatca gcgccaaaat ctgcgcaatc tgcgggaaat
    14221 acatcgccga tttggaaatc gatgttacgg ggtccggcgc ttcggagaat aaatgagcac
    14281 catcattgta aatggccaca cgattccctg ggaggatgga atgaccgtcc gcagggtatt
    14341 gcaggtgatg aactacagct tcagaatgct ggtaatcaag atcgacggca agctggtgaa
    14401 acgtgccgat tatgaccaca ctttggtacc agccggttcc gatgtgatgg tctatcacat
    14461 gatcagtggc ggttgagtga gattgaccaa aaaaatcctt gaccaattaa gcgtaagttt
    14521 taattgggtg gaatatgact aaatggagtt atgaatgaat atccacttcc gtgatgttca
    14581 gacaggaaac gtggaagccc gcgcgatctt ggagatcgcc gacggggtct ttttgaacga
    14641 gatcactatc ctcaactacg aaggccagat cacggtggag tttccccgta agagctttgt
    14701 gggcaagaat aaacgcacct tttacctcga tatcctcagc tttgaggaca acgacaaacg
    14761 catcgtctgg gagttggaaa tcaaatccgc ttacaacgaa tggcgcaaga gcaataaaaa
    14821 gatcctgatt tataacaaaa gtaacataga tggaggatcg tcatgatcac cgatttgcaa
    14881 gccacgcttt ccaccaacat caagggtatg aagcgttccg ctatccgcga gctgctcaag
    14941 tttttgggcc agcctggtct gatctccttg tcaggaggtt tcccttcacc gctcaccttc
    15001 cccaccgtcg aactcaaaga gatcatgtgt gaggtaatgg acaccgaagc cgctttcgcg
    15061 ctgcagtatg gcgccacgga aggtgacacc ctgctgcgtt ccctgctggt aaaccgttac
    15121 aaggccgccg gcgtggatat gagcatcgaa aacatgatca tcactactgc cagccagcaa
    15181 gctctggacc tgatcgccaa aatcttcatc aaccgtggcg acagcgtaat cgtgggcctg
    15241 ccctcatatc tgggaggcct cagtgccttc aattcctacg gcgccaatat gatcggcatc
    15301 ccgatggatg atgagggcga agacgtccag atcatggaag aaaagatcaa agaacagatc
    15361 gcccagggca acaaacccaa attcatctac gtgatcccgg attttcaaaa tcccgccggt
    15421 atgacgatga gcgaacgccg ccgcaaggaa gtgatcgaca tcgcccaccg ctatgatatt
    15481 ctcatcctgg aagacagccc ctatcgcgag cttcgctacg agggcagcca ccaaaaaacc
    15541 atctatgagc tggaaggcaa tggacaggtg atcctgctgg gcactttctc caagatcttc
    15601 tgccccggtt tcagaatcgg ctgggtggtg ggccatccgg atatcctgga taaaattgtc
    15661 gtcgccaaac aggccaccga tctttgcacc cctcccttca cgcaacgcat tgccgcccgc
    15721 tacatcgaaa aaggcctcct ggaccccaaa atccagcaga tccgcgaaat gtacagcgtc
    15781 aaacagcagg gcatgctcaa atccctggcg gagtttatgc cggaggaatt tagctggact
    15841 catccggaag gcggcctttt cctgatggcc aaaggacctg agtggatcga taccaacgcc
    15901 atgttgctgg attgcatcaa aaacgccaat gtcgcgtatg tggctggcac ttccttcttc
    15961 tgtgacggtg gtggccaaaa cactatgcgc ctcaacttct cctacgagac cctggacacc
    16021 aaccacgaag gcgccagacg cctgggtaac tactacaaag acgtgatcgc caagcataaa
    16081 taagcaacgc aaggactttc tcccctggtc agtagcgaag ccggaccggg ggagagtttt
    16141 atatgaaagc aaatacaaat aagataaatg gaggaatgat gcctaaaaaa gtcgaaaaag
    16201 acaaaatggg catgatcatt cgagaagaag acgctctgga aaagatggaa gtggagatca
    16261 ccgaagtcaa agacccgggt gataccgact ctcccgtatt gatttattac aactggtgca
    16321 aaaagtgcgg aatctgcgtg gctttttgtc ccacaggctg tttgggacgt aaagcggacg
    16381 gctcgccata cgtggcatcc cccgaaaagt gcattcactg cgaaacctgt gaccgtctgt
    16441 gtccggattt tgccataacc ggcgctaaag accgctaagg agatgaacat gccaagtccc
    16501 aagaaaactg ccattgccaa accccgcggc aaagcgtcca agactgtcaa actccccaag
    16561 gccactgagg agacccccaa agtggaaagt cctctcaaag agctggaagc caagcgtgaa
    16621 cgcaaaaatg tgctcatgca gggcaacgag gcggttgctt ttggcgctct cgatgccggg
    16681 tgtaacttct tcgccggata tcccatcacc ccgtccaccg aaatcgccga aatcctggcc
    16741 ctggagcttc ccaaacgtgg tggaaccttt gtgcagatgg aagacgaaat cgccagcatt
    16801 tgtgccatca cgggagcctc tctggccggc gctaaggccc tcactgccac aagcggtccc
    16861 ggcttttcgc tgatgcagga aggcataggt tttgccaaga tcaccgagac tccctgtgtg
    16921 gtcgtaaacg tccagcgcat gggtccctcc accggaatgc ccaccagtcc agcccaggga
    16981 gacatcatgc aggccaaatg gggcagccat ggtgacagcc ctgccatcgt gctctatcct
    17041 gacagcgtca aggagagcta cgagctcacc atccgtgcca tcaatcttag cgaaaaatac
    17101 cgcacctgcg tgatcctttt gttggatgaa gtgctgggcc acatgcgtga atccgtcatg
    17161 ctgcccgata tggcaaatgt gagagtgatc agcaggatca agcccactgt tccgcctcac
    17221 tggtacaagc actacgacga aaaccagaag tatctttcgc ccctggccag ttatggcgag
    17281 ggctaccgct tccacgtgac cggcctcacc cacgacgctt tgggatttcc caccaataag
    17341 gccaccgaag ccgctgaaat gatggaacgc ctgcgcaaga aaatctccta caacatccgt
    17401 gatctggtgc agatcgagag ccatcagatg gaagatgcca ccattgctct gttcgcggcc
    17461 ggcatcacag cccgcagcgc taaagccgcg gtggccatag ccagaagcga aggcgtcaag
    17521 gtgggcctcc tcaggccgct cactatttgg cccttcccgg acgacgcggt ccgcaagatg
    17581 ctccaaaacg ttgaaaccgt tatagttccc gagctcaatc agggccagct gatcaacgag
    17641 atcaagcgcc tcaccaagaa taagagcgac agcaacgtga tcgggcttca gagagtgaat
    17701 ggcaagctca tcactcccaa cgacatcctg cgcaagatca aggaggtgat gtaatggcca
    17761 atattccaca acgcgtgatt cacgactatc tgcgccacga taaaaagttt ccccatgtct
    17821 ggtgcgcggg ctgcagcaac ggtatcgtgc tgggttccat catccgtggg atcgccagca
    17881 tgcagatcga ccgtgacgac atcgtgatgg tctccggtat cggatgctca tcccgcatgc
    17941 cggtctatgt cgatttcaac accctgcaca ctctgcatgg acgctcaatt gcttatgcca
    18001 caggcgtcaa gcttcataaa ccgcatctca aagtgatcgt gatcaccgga gacggcgatt
    18061 gcacggccat cggtggcaac cacctcatcc atgccgcccg ccgcaatatc gatctcagcg
    18121 tgatcctcat caacaacaac atctacggca tgactggcgg acagtgttct cccaccaccc
    18181 ctcacgacgc tatcgccacc accgctccct acggaaacat cgagccggat ttcaatattt
    18241 gcgagctggc cttaggcgct ggggccactc atgtggcccg caccaccgct ttccatactc
    18301 aggaagtgca aaaaatggtg gtggaagcca tcgaacatcc cggcttttcg ctgatcgaag
    18361 tggtgagcgc ctgcccggtg atctttggac gcctcaacaa gaaaggcggt ccggcccaga
    18421 tgatgaaaga catgcgcgac ggctccattc ctctcaccgc ggtggataaa ctcccccctg
    18481 aaaaggtgga aggcaagatc atccgcggca cccttcgcaa ggacgtcaaa ccggaattct
    18541 gcgctgccta tggagagctc caaaagcgcg cccaggctat gggagatgtg aaatgaagaa
    18601 ctatgagatc agactttcgg gcagtggcgg tcagggactc atcctggccg gcatcatcct
    18661 cgccagggct gccgtgatcg ataaacgcaa ggtcactcag acccagagct acggtcccga
    18721 atcccggggc ggttcttccc gggccgacgt gatcatcagc gatcacgata tctatttccc
    18781 tgaggcgatg aactttaact gcctgctggc cctcactcag gaagcctgtg acaagtatct
    18841 ctttgacctg cgggaagaag gggtcctgat catcgacacc acctttgtca aaaacctcgc
    18901 cctggcggca gaaaacacct atgaactgcc cttcacagac atcgccatcg ataagctggg
    18961 cagcgccatc agcaccaaca tcctctcgct gagcttcctg gtccgtctca ccaaagtggt
    19021 caaagaatcc tcgttggagc aatccatccg cgaaagcgtg aagcctgcct tcatcgatct
    19081 caacctcaaa gccatgcaca tcggctttga actggccgac aactacaaga agtaggagcg
    19141 cttatggtga agcatatcag tcacataggc atagcggtaa aggacctgga cgccggcatc
    19201 gagttctata aaaaactcgg gctggaactg gaagctgtgg aagaagtgcc ttcccagatg
    19261 gtgaaggtgg cctttttccc ctgtggtgac acccgcatcg agctcctggc ccccacctcg
    19321 gaagagagcc ctgtggccaa attcatcgag aaaaagggcg aaggcatcca acacattgcc
    19381 ttcgcggtgg atgacctgcc cgctgccctg caggaatcat ctgacaaagg catcaccctc
    19441 atcgacaagg aaccgcgtcc cggtgcccat catgccgata tcgctttcct gcatcccaaa
    19501 tcctcgatgg gagtgttgat cgagctctgc aaagagaagc actaaactta tatcattgta
    19561 atgacataga gcggacctga agagggtccg ctctttgcgt aactcaagct ttagagctag
    19621 tgcgaatatg gcgtaatgag tcccatcggg acggtagttt agctagatat gcgagg
//
LOCUS       DAOSFN010000027        19395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2279_length_19395_cov_8.244881, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000027 DAOSFN010000000
VERSION     DAOSFN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 19395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19395
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2279_length_19395_cov_8.244881"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /gene="ybgF"
                     /locus_tag="PLO35_03140"
     CDS             <1..1197
                     /gene="ybgF"
                     /locus_tag="PLO35_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOH78690.1"
                     /translation="AELKAGHDAGMADLQTQISTNMDEIRLELDMLAEDTDFSLEAYT
                     DNLAELRSVSSNFATKDELSAVINENEKVTATLTGLTKEVQDLSQSNLGKASTLEADV
                     QDLRDMVLDMYAEFTAAGMVGSEEMEAKLGVESQKRAALMNDLLARLSEVESDIKGEI
                     SSEMGVRDESLQGISGRLDDIEMEIEVMREENSAWIASLREDQELQVTTLKTEVNDML
                     TTTKAEITTSNNTIKTDVSSLRQRVESLSTEVKGVSTDLEDVIVQERAKKEKRRQLSI
                     AAQYKAALAAYNKRKYEESIVMFEDFLAQYPNESLSANAYYWIGENYYAGKKFSTALD
                     SFIYVRDNFPTHHKVPDAMLKVGMCYYNLDMKYQANDEFTKLKTSYPKYNRMDLVNKY
                     LRLTAN"
     gene            1201..1794
                     /locus_tag="PLO35_03145"
     CDS             1201..1794
                     /locus_tag="PLO35_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78691.1"
                     /translation="MPRYLWLVFCAALWISVAQAQISPVEITAISQSIAARVITGEPI
                     ILEIRAGEWSQALANEIRRQLLIQGADIREAGLGQHSPAISYTEGDSSEVEEYDLRPL
                     KLDAALLVLVEMELRWQTVERGGFLSYSSQRKPVYVFNLKQISLPTRQLRHVSSLSHE
                     FQSGEQGGSVITGIKWFEPMLVTAALASIIYLLWTTE"
     gene            1813..2547
                     /gene="bamD"
                     /locus_tag="PLO35_03150"
     CDS             1813..2547
                     /gene="bamD"
                     /locus_tag="PLO35_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOH78692.1"
                     /translation="MRTRQFLILTLVIGMLLTACAKDAAFQSSDEKLAKADELYANKK
                     YARAAELYGEIYFERSSASTAYALMRQADSYFAINRFADARAAYEEFINTFPKHQDVS
                     TAFFQSALCMLEESLPAQYDQGETVAAIASFRRFIEKYPGDPRYEQAIEYIRKAQGKL
                     IEKRYQNGYISYKMKDYSAALMYFKEVTDLGNTNDPDRMSLYYSAIICQRQNRQEEAK
                     SFYQLLVDKYPQSKEARKLKGLFSKQ"
     gene            2544..3176
                     /gene="nadD"
                     /locus_tag="PLO35_03155"
     CDS             2544..3176
                     /gene="nadD"
                     /locus_tag="PLO35_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOH78693.1"
                     /translation="MKTSHGRAFDHNNKEERDQQRLTALIGGSFDPIHNGHLHLAREL
                     LKDPLISVVAFLPVGRHHFKGDRAVLSYSTRAALIDRVLEPGMELWEDDASGNGFTAD
                     LIRRLRIRYPEKRFAFVIGSDNLSQLPLWREYEWLRENLFFIVIPRPGYDLRMPEPAP
                     RCQIKAIDAPDISSSQIRALIASGQPLTGLLPQCIEEDVVKLYTQGEQHP"
     gene            3173..3892
                     /locus_tag="PLO35_03160"
     CDS             3173..3892
                     /locus_tag="PLO35_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOH78694.1"
                     /translation="MSKSKPAGSQAGKQPSGPKDKSRFLDRVPLSQKQSLGEEIANSV
                     THGTGVGLSIAALVILVVFAARQSDVWKVVSFSIYGATMIALYLASTLYHAFPQPRVK
                     AFFRILDHSSIFLLIAGTYTPVTIGELRGPWGWTLFGVVWLLTIIGINLKIFAMGKLK
                     ALSVVIYIMMGWMVVLIINPLRQQVPPQMIAWMLAGGLCYTLGVVFYVFKKLPYHHAI
                     WHLFVLGGSVCHFFGMLTLVK"
     gene            complement(3967..5304)
                     /locus_tag="PLO35_03165"
     CDS             complement(3967..5304)
                     /locus_tag="PLO35_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH78695.1"
                     /translation="MRNLHLFMLLLAVGLLASSLNGTPQSVFFGQSGFDPSPQTFALP
                     STRQTVMNSLLYQDYQADWQDNYLFSAELNDQGRITAITSSGWNPDSALWEEQARQII
                     EYRPDFQPAHIAMTWLKDGEWSLFAEVNYVYEDDLVRMISYNQFEDGESRPYWQLAFY
                     YTPNTLILNSVLEFQYMGEPVPLNIKKYDYIWDAGSRPSEILSSVMSLTREWEPTTRD
                     TYVYHNDDQTTHASYLRYLEIGWPFNTQLLTGVFPTKLLEHRRFQRYAPTSDWYETVR
                     EFYSYDASNQLETIDQYESPASTVWEVNQRRTYSYTDGLPVSETWWHDPDGQDVLVPS
                     SRIIYGYTQITAADDPVAPPAISDLKVFPNPFNPQTAIGFKLQDSANMEVSVYNLKGQ
                     RVRKIFAGNASSGDHNLNWDGRDDQGRKLAAGIYLISLKAGNESDTVKAVLSR"
     gene            complement(5531..8923)
                     /gene="mfd"
                     /locus_tag="PLO35_03170"
     CDS             complement(5531..8923)
                     /gene="mfd"
                     /locus_tag="PLO35_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOH78696.1"
                     /translation="MILPLILSDLNRQSFAMELLAALDSGQLVQAYHMNQSARALMAA
                     WLWNQTGKNVLIVSQDDIIAEDIWDDLVAFIGRDKARYLPDYEILPYEERSPHYSIRA
                     TRMICLQQSLDPAPAVYSLSIRALTRYLPSRHLLERQIFSLVRGMEITPEELTTRLFN
                     MGYEHEYQVTKVFQMSRRGGIMDIFSPPSLKPVRLEFFGDEIVSMRVFSTNTQRSEPG
                     EIDSFTVIPARELCLDDIDPHSPFASQIKKKGFYEGIENHYGLLLPELSTFADYFASE
                     DRLLLFNNFSYVWEEFEQLQEQVHTQYRKELKHKTKARLTPPERVLAGEEHLFELLKP
                     GKAMLLSQSEFSLGIANQPLKTVFEPQPAFGGDLDLLASSLRENAREGRRSILLFDNQ
                     SQSQRTKLLLADYEAEAEHHIGVLHEGFGIGLTGPVLWTDHEIFNRYKRKRYTPRFSP
                     GEEIVDYEALKPGDYVVHIEHGIGVFEGLKIIRLDAQDTECLVLRYANDDRVYVPTYQ
                     LKMVTKYVAEEGASPTLHRIGSARWQNTKRKAAQQIELVAADIVRLYAERSARVGFSH
                     KPDTDWQRELEESFIYEDTPDQARTSLEVKADMELPAPMERLLCGDVGFGKTEVAIRA
                     AFKAVCSGTQVAVLVPTTLLAEQHWRVFKERLAQYPIRVAMLSRFRAKNLLARDIDEI
                     LSGKVDIAIGTHRLLSKDIRFPNLGLLIIDEEHRFGVRHKERLRKLQSNVDTLYMSAT
                     PIPRTLNMALAKLKEISLMQTSPKERLPIRTIITPRDMEVVRDAIRREVDRGGQVFFI
                     HNRVQTIEHVADELRNLMPNVRFAVGHAQMSEHHLEDVMDAFIAKEFQVLISTTIVEN
                     GIDIPNANTILIDRADTFGLAQLYQMRGRVGRSNRRAYCYLLIPRGTTTEARHRLEAL
                     TQYDFLGAGFQVALRDLELRGAGAILGTKQSGIIQSIGFNYYNSLLEKAISSGSGGGQ
                     VSLEEELQKSPGAKLKTEIDLYFPPEYIDNDEQRLGIYRRLSAFEKVEEIEEMERELI
                     DRFGPMPEKASWLLMFFKMDLMANQAQLANCKVRNHTFTLEWRADQIPSKQKVLAFGA
                     RVALPMSFDTSKGLRIEIGMDRDHGYREQFETALGILKGYLE"
     gene            complement(9181..11379)
                     /locus_tag="PLO35_03175"
     CDS             complement(9181..11379)
                     /locus_tag="PLO35_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOH78697.1"
                     /translation="MNIDFEGQLNDAQLRVVSYVQSPLLVLAGAGSGKTRCIIYRCAH
                     LIKNLGVAPWNILVVTFTNKAARELQERLEKLLGFPVSSLWVGTFHSLCARILRYEHA
                     YLPFSSSFSIYDDDAQKSLLKKIYKEHGYDVKKYPLNRVLSRISRYKNRLQLPEDLSD
                     SASSLSHADPFHKGVLDIYKLYQQALLFNQAVDFDDILLYTARLLQDNDTVRAKYANQ
                     FKHVMIDEYQDTNHAQFEIIRQIAGGHHKVCVVGDDDQAIYSFRGATIRNILEFEKDY
                     PEVMAIRLEQNYRSTMSILNLANAIIKKNRRRHSKDLWSERGEGLKPRLNIYEDENDE
                     AEKVAQTILTLREQGIPLGQIAVLYRTNAQSRVFESAFMQYRIPHILVGSLHFYQRKE
                     IRDLLAYLNVISNPADSESLLRIINEPPRGIGQTTVNRVIGYANSLRIGIRDSLHAVD
                     AISDLAAAAKKKLAGFSHMLDTWRDAARTKAAPDLVRLVIDELQLIDQYRKGNDPKEI
                     ARAENLMEFVNSVTEFCERYQKENERVPLLADFLPYVALQTDLDRVKDGEEALRLMTL
                     HNAKGLEFSHVFIVGLEQELLPHRMSMGSREEIEEERRLFYVGVTRAKDNLSLSLARQ
                     RRLYGVFDHCQPSMFLQDLDAALFQGSTDESSYAPRPQPRLKTRAQITDKDKHYRIGQ
                     DVWHDDFGHGKVLSVNGTGRDATLAVSFDKGSLLKVIGTFLRTESSPDER"
     gene            complement(11436..>11786)
                     /locus_tag="PLO35_03180"
     CDS             complement(11436..>11786)
                     /locus_tag="PLO35_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78698.1"
                     /translation="NPLDPNADPTIIVPEIISNLQVFPSAPGAASKYVDLTWQANPEY
                     STDGYYVYRGLGFYSAFAVVDTVYTNRASHGSKPWHSVVPGEYYYKISAFKQYSGGRL
                     EGRPCLPVWVRIPI"
     assembly_gap    11787..11886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11887..12401)
                     /locus_tag="PLO35_03185"
     CDS             complement(<11887..12401)
                     /locus_tag="PLO35_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78699.1"
                     /translation="MSRRLVLFLLLLGCQYGWAQSFDINAFSDSTKYGWQTYLERNAY
                     RQDLLERQDLLQLYEMEANSLRDCVIKSAIAPGWGQFVNKQSTKGSVFLATELVLAGA
                     SLYFYDRSMYYYNRYLDASQVEDIESYYNAAVGPRQYSLIFLGVGALVWIYNIFDVIQ
                     GTEEYNAQVWK"
     gene            complement(12398..13798)
                     /locus_tag="PLO35_03190"
     CDS             complement(12398..13798)
                     /locus_tag="PLO35_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78700.1"
                     /translation="MRYSAALLAIILVTISACEINRLRSAEEHYGQKRYAAAIQESDE
                     FILKANNGALRTRAELVRSNSYYQLGLIAVQRNSIDLAIRFFKLSNSSQADFELAKIY
                     KSRLLEAATVGDFETQLLYLNSILREIPTSDLVPEMINRRIVIYLDQIGDADAAWHDY
                     MNLFDTYPGNSYEVAARKSMERIVPNKIAYGERLANAGYFNEALAVYFELAKYPIIRT
                     EEINALIGSTYRGQAELYLDQENYLEADRFLRIALQYIPEQRPQIEERLRQIVDLFIK
                     KGDAFLAQRDFENALTHYQRVFEIIPDYPPARQAIERVRITQENIARAAQLFRQAELT
                     EASGKVADALKLYQQANGLDSNPETRTRISIMQNTIEADRNPLAFARKIINEYRNGLL
                     NNRITQQKKQLLGRYKNSEIRDSGWKFLLSTGQYKYEARYDLMTPQETFLYVWQVSLK
                     DRKIIPLNKISEELMK"
     gene            complement(13795..14538)
                     /locus_tag="PLO35_03195"
     CDS             complement(13795..14538)
                     /locus_tag="PLO35_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH78701.1"
                     /translation="MNFIILGAGPGLPLPDKCLSSIYVNHGDRHFLFDCGEGCSRQLL
                     RHGLSGEVLDAVFISHFHPDHISGIFMLIQMLYLQKRSKDLFLFLPERETEFESLLQY
                     QYTFLEKLGFKLHILSNDTIRDHFPQIHAMQTDHLWGYEELVTALDLPNQMRSWAFRI
                     GEPGGDLVYTADIQSTDCVASLLQGCHTAIVDAMHPPLEQILKLADNGIPRVLLTHGL
                     SEELELWLQHHQADNFELAMEDIGYHIEL"
     gene            complement(14535..15635)
                     /locus_tag="PLO35_03200"
     CDS             complement(14535..15635)
                     /locus_tag="PLO35_03200"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOH78702.1"
                     /translation="MENRIYEYLSVFIMSWMLVYGLTPFIMRLARATNLVDKPNERKD
                     HPKVTPLMGGLSVFIGFFLLCLYDVAISPGRYFDKPMMGYLAGSLLIMLIGLWDDKRA
                     MQPLIKMLGQIVVSFIFILSNFTLTELGLMFGSIYISIPLMVLWMVGLMNALNFLDNM
                     DGILSGMAGILGLGFFAFSLTTTTQTNQESMALIGLISLSFAGSVFGFLPYNFNPARI
                     FLGDAGSMFIGYFLSSMGILMARLAGNRYNDKMFFLMPVLLLSYAIFDIALVSYTRGR
                     DGRHVMQGGQDHSTHRIRNALGSIKVTAFIIYSINVLIVLTSIIVFTIGSRELLLITT
                     ILFASFFLFFGRKLDQVPVVIPENQKKTGKIK"
     gene            complement(15735..16508)
                     /gene="lpxA"
                     /locus_tag="PLO35_03205"
     CDS             complement(15735..16508)
                     /gene="lpxA"
                     /locus_tag="PLO35_03205"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOH78703.1"
                     /translation="MSTIHPTAIVHPDAVIGNDCVIGPHCVIGPQVKLGERNQLAPFV
                     IIDGDTTIGDDNRIFSHAVLGTDPQDLKFSGESTCLRVGSRNTIREFVTINRSNHAGE
                     DTIVGNDNLLMEYVHIAHNCQIGSFCIIANIVQLAGHVIIHDYATIGGATAIHQFVHI
                     GTHAFVGGASAIKKDIAPFTRGQGNPYKTVGLNSVGLTRKGFSNQSVAAIKEMYNLFY
                     RKGLNTSQALAVADSRTDLLPEQQTFVEFVRSADRGLST"
     gene            complement(16505..17902)
                     /locus_tag="PLO35_03210"
     CDS             complement(16505..17902)
                     /locus_tag="PLO35_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOH78704.1"
                     /translation="MTEYKHTIQHGVSYTGIGLHSGEISTIRFKPAGKDDGIVFIRVD
                     LPDKPEIPADIDHVVDISRGTTIGINGAVVGTIEHVLSAIKGLNIDNVRIEIDGPEAP
                     VADGSPIVFLNLLRQAGLVQQDSERVFFELDAPISFSAPEENVDIVIVPSNELKVTFM
                     IDYKHPNLGTQYTWLPSLDYYEREFAGARTFCFINEILYLKEKGLIKGGSLDNALVIA
                     EPNMSEEELTHLQQVFNYHKPVQVSEEGILNNNKLRYYNEFVRHKVVDLLGDIALLGM
                     PIRGHILAARSGHKTNVELVKKLRQIQKKNELQMMYQKKRNQEAVFDINAIMRIIPHR
                     YPFLLLDKIIDFVPNESITGIKNVTINEPFFQGHFPDHPIMPGVLILEGMAQAGGIML
                     LNQLENPRDYVAYFASIDNVKFRKPVLPGDTLRYEMKVISLKRSLSKMHGEAFVGNEK
                     VAEGDFMAMLQEKNK"
     gene            complement(17939..18964)
                     /gene="lpxD"
                     /locus_tag="PLO35_03215"
     CDS             complement(17939..18964)
                     /gene="lpxD"
                     /locus_tag="PLO35_03215"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOH78705.1"
                     /translation="MKKLSPSLGTAQLNMLPQASYQGDPELRIDSVSTPEDAGPGSVV
                     FLEQEKLLETTQASQASLIITTPEFALKLEGRNLLITPKPYITMMTLLSYWLSMAPKK
                     PAGIHPSAVIDPSATVSETTRIDAGVRIGANARIGSNCRIDANCVIADNVVIGDGCIL
                     YPNVVIYEDCVIGREVIIHSGAVIGADGFGFALLEGVQRKIPQIGNVILGDYVEIGAN
                     SCIDRATLGSTVIGDGTKLDNLVQIGHNCVIGRQCILCAAVGLAGSTVVGDYVYLAGQ
                     VGAAGHLTIGDRAMVGAQAGITNHIPADSKYFGSPATEANQMKRIMAAQKHLPDIYRF
                     YLKNKQE"
     gene            complement(18966..>19395)
                     /locus_tag="PLO35_03220"
     CDS             complement(18966..>19395)
                     /locus_tag="PLO35_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOH78706.1"
                     /translation="EIDKLTKNEAAKRDALAKIDARKVEAGRLLQEYFGEGGKAEQRY
                     RELIDPLTLKIQEVIVKVAQDEKYTMIFDSSYPTILYAIPSIDITDIVLLEINKDTIA
                     PGTEADTELKPDETKPEPEFKDPMPGDVTNPNETKPPIKP"
BASE COUNT         4319 a         5037 c         5394 g         4545 t
ORIGIN      
        1 gccgagctga aagctggtca cgatgccgga atggcagatc tgcaaaccca gatcagcacc
       61 aatatggatg aaatcaggct cgagctggac atgctggcgg aagacactga ttttagcctg
      121 gaagcttaca cggacaacct ggctgagctg agaagcgtat ccagcaactt tgccaccaag
      181 gacgaactct ctgccgtgat caacgaaaac gaaaaggtca ccgccaccct caccggcctc
      241 accaaggaag ttcaggacct cagccagagc aatctgggca aggcctctac cctcgaagcc
      301 gacgttcagg acctgcgtga catggtgctg gatatgtatg ccgaatttac cgcggctgga
      361 atggtcggtt ccgaggaaat ggaagccaag ctgggagtag aatcccagaa acgcgccgct
      421 ctcatgaacg acctgctggc ccgcctcagt gaagtggaat ccgatatcaa gggtgagatc
      481 agctctgaaa tgggagtccg tgacgaatcc ctccagggga tcagtggccg cctcgacgat
      541 atcgagatgg agatagaggt tatgcgggaa gagaattctg cctggatcgc ctctctgaga
      601 gaagatcaag agcttcaggt caccactctg aagaccgagg tcaacgatat gctgaccacc
      661 accaaagcgg agatcactac ttccaacaac accatcaaga ccgatgtcag cagccttcgc
      721 cagcgggtgg aatcccttag cacggaagtt aagggagtca gcaccgatct ggaagacgtg
      781 atcgtgcagg aacgcgccaa gaaggaaaaa cgtcgccagc tcagcattgc cgctcagtac
      841 aaagccgctc tggccgctta taacaagcgt aaatacgaag aatccatcgt gatgtttgaa
      901 gacttcctgg cccagtatcc caatgaaagc ctttccgcca atgcctatta ctggatcggc
      961 gaaaactact atgccggcaa gaaattcagc actgctctgg attccttcat ctatgtccgc
     1021 gacaacttcc ccacccatca taaagtgccg gatgccatgc tgaaggtggg aatgtgctac
     1081 tacaatctgg acatgaagta ccaggccaac gacgaattta ccaagctcaa aaccagctat
     1141 cctaaataca accggatgga cctggtaaac aagtatcttc gcctgactgc caactaagag
     1201 atgccgagat acctgtggct ggtcttttgt gccgcgctgt ggataagtgt tgctcaggcc
     1261 cagatttccc cggtggagat cacggccatc agccaatcca ttgcggcgcg agtcatcaca
     1321 ggtgaaccga taatccttga aatacgggcc ggtgaatgga gccaggccct ggccaacgag
     1381 atcagacggc agcttttaat ccaaggtgcc gacatccgtg aagccgggct gggccagcat
     1441 tcaccggcca tttcttacac ggagggagac agcagtgagg tggaagaata tgatctgcgg
     1501 cccttgaaac tggatgccgc cctactggtg ctcgtcgaga tggaattacg ctggcagacc
     1561 gtcgaacgcg gtggtttcct ctcctacagc agccagcgaa aacccgtgta tgttttcaat
     1621 ttaaagcaga tatcgctgcc cacgcgccag cttcggcacg tttcatctct cagccacgaa
     1681 ttccagagcg gcgagcaggg cgggtcagtc attaccggca tcaaatggtt tgaaccgatg
     1741 ctggtcacag ccgcgctggc ttcgatcatc tatctgctat ggaccactga atagagcaag
     1801 gaacccccaa acatgcgaac cagacaattt ctgatcctca ccctcgtgat tggcatgcta
     1861 ctgacagcct gcgccaaaga cgccgctttc cagagcagtg acgaaaaact ggccaaagcg
     1921 gatgagctgt acgccaacaa gaaatatgcc agagcggccg agctgtatgg agagatctat
     1981 ttcgaacgtt cttccgccag caccgcctat gcacttatgc gacaagcgga cagctatttc
     2041 gcgatcaacc gctttgcaga cgccagggcg gcctatgaag agtttatcaa cacctttccc
     2101 aagcatcagg atgtgagtac agcctttttt caatccgccc tctgcatgtt ggaagaatcc
     2161 ctcccggccc aatatgacca gggtgagaca gtcgccgcca ttgcttcctt tcgccgcttc
     2221 atcgaaaagt atcccggcga cccgaggtat gagcaagcga ttgagtatat ccgcaaggct
     2281 cagggcaagc tgatcgaaaa acgctatcaa aacggttata tcagctacaa gatgaaggac
     2341 tacagcgctg ccctgatgta ttttaaagaa gtgacggacc tgggaaacac caacgatccc
     2401 gaccgcatgt cgctctacta ttcggccatc atctgccaac ggcagaacag gcaggaagaa
     2461 gcaaagtcct tttaccaact cctggtggac aaatatcccc aaagcaagga agccaggaag
     2521 ctcaaaggtt tgttttcaaa acaatgaaga ccagccacgg cagggcattc gatcacaaca
     2581 acaaagaaga gcgggaccag caaagactca ctgccctgat cggaggaagc tttgacccca
     2641 ttcacaacgg ccatttgcac cttgcccgcg aactgctgaa agatcccctc atcagcgttg
     2701 tagcctttct tcccgtgggc cgtcatcatt ttaaagggga ccgcgcggtg ctcagctatt
     2761 ccactcgggc agcactgatc gacagagtgc tggaaccggg tatggagctc tgggaggacg
     2821 acgcgtccgg aaacggtttt acggcagacc tgatcaggcg gctccggatc agatacccgg
     2881 aaaaacgctt cgccttcgtg atcggttcgg acaatctctc ccaactaccg ctctggcgtg
     2941 agtatgagtg gttgagggaa aacctctttt tcatcgttat accaaggcct ggctatgatc
     3001 tcaggatgcc tgagcctgct ccccgttgtc agatcaaagc cattgacgct cccgacatct
     3061 catccagcca gatcagagct ttgatcgcct cgggacagcc tctcactggt ctgctgcccc
     3121 agtgcataga ggaagacgtc gtaaagttat acacccaggg ggaacaacac ccatgagcaa
     3181 atccaaacct gccggctccc aggctggcaa gcaaccttcg ggcccaaagg ataagtccag
     3241 attcctggat cgcgtccccc tcagccagaa acaaagtctg ggtgaggaaa ttgccaacag
     3301 cgtcacgcac ggcaccggag taggtctgtc gatcgccgcg ctggtgatcc tggtggtctt
     3361 cgcggcccgt caaagcgacg tctggaaagt ggtctccttc agcatctatg gcgccaccat
     3421 gatcgctcta tatttggcct cgactctcta tcacgctttt ccccagcctc gtgtaaaagc
     3481 cttcttcagg attctggacc actcctccat ctttttgctc atcgccggca cctatactcc
     3541 cgtcaccatt ggagaactga gaggcccctg ggggtggacc ctctttggag tagtctggct
     3601 gctcaccatt atcggcatca acctcaagat ctttgccatg ggaaagctca aagccctgtc
     3661 cgtggtcatc tatatcatga tgggctggat ggtggtactg atcattaatc cgctccggca
     3721 gcaggtaccc cctcagatga tagcctggat gctggctggc ggcttgtgtt atacactggg
     3781 agtggtgttc tatgtattta aaaagctacc ctaccaccat gccatctggc acctctttgt
     3841 gctgggaggc agtgtttgcc acttctttgg catgctcacg ctggtgaaat agttcaataa
     3901 agctgaggcc gcccaccagg cggcctcaat tgtcatgatt ctgggtttgg agcctgatta
     3961 caccagttat ctggacagca cggccttcac tgtgtcgctt tcattacctg ccttcagact
     4021 gatcaggtag attccggctg ccagtttgcg gccttggtcg tctctgccat cccagttcag
     4081 attgtggtct cctgagctgg cgttgccggc gaagatcttc cgcactctct ggcctttcaa
     4141 attgtagacc gagacctcca tattggcaga gtcctgaagt ttgaagccga tggcggtctg
     4201 aggattgaag gggttgggaa acactttcag atcactaatg gcaggagggg cgacggggtc
     4261 gtcggcagcg gtgatctgag tatatccata tatgatccgc gagcttggca ccagcacatc
     4321 ctggccatcg ggatcatgcc accaggtctc ggataccggc agtccgtctg tgtagctgta
     4381 ggtcctgcgc tggttgactt cccagaccgt ggaggcgggg ctttcatact ggtcaatagt
     4441 ttctaactga ttgctggcgt catagctata gaattcccgg acggtctcat accagtcgga
     4501 agtgggagca tatctttgga aacggcgatg ctcaagcaac ttggtaggga agacaccggt
     4561 gagcaactgt gtgttgaaag gccagccaat ttccagatag cgcaggtagg aggcgtgggt
     4621 ggtctgatcg tcgttgtgat agacgtaggt gtcgcgggtg gtgggttccc attctctggt
     4681 caaagacatc acggagctga gaatctcgga tggacggctg cccgcgtccc agatataatc
     4741 atactttttg atattgagcg ggaccggctc tcccatatac tgaaattcca gcacggagtt
     4801 caggatcagg gtgtttggag tgtagtagaa ggcaagctgc cagtagggac ggctctcacc
     4861 gtcctcaaac tggttgtaac tgatcatccg gacgagatca tcctcataga catagttgac
     4921 ctctgcaaac aaactccact cgccgtcttt caaccaggtc atggcaatat gggcaggctg
     4981 gaaatcagga cggtattcga tgatctgcct ggcctgctct tcccagagag cgctgtctgg
     5041 gttccatccg gagctggtga tggcagtaat ccggccctgg tcgttgagct cagcgctgaa
     5101 cagatagtta tcctgccagt ctgcctgata atcctgataa agcagactgt tcatgacggt
     5161 ctggcgggtg gaaggaagtg caaaagtttg gggactggga tcgaagccag actggccaaa
     5221 aaagacgctt tgcggagtcc cattcaggga cgaggccagc agtccaacgg ccagcagcag
     5281 catgaaaaga tgcaagttac gcatgataac ctcctcaagg gttggcgttt gataatcttt
     5341 gaatattgca tccaagcctt gatgatatag taacacatcc cagccaaaag acaaatgtta
     5401 aatttcatag acgctgttga tagatttcta caatatgctc tttgcccttt attagtgcag
     5461 gctaagaccc cataaaaaaa tcttcgccgg cttgctccgc tccagctgca acacgcccaa
     5521 tcacttgcat ttattccaga tagcccttca ggattccaag ggcggtctca aattgctccc
     5581 ggtagccgtg atcgcgatcc attccgatct cgattctgag gcctttggaa gtgtcgaagc
     5641 tcataggtag tgccactctg gcaccaaagg ccagcacctt ttgttttgag ggaatctgat
     5701 cggccctcca ttccagcgtg aaggtatgat tgcgaacctt gcagtttgcc agctgggcct
     5761 ggttggccat caggtccatc ttgaaaaaca tcagcaacca ggaagctttt tccggcatgg
     5821 ggccaaagcg gtcgatcagc tcacgctcca tctcctcgat ctcttcgacc ttctcaaaag
     5881 cactgaggcg gcgataaata cccaggcgct gttcgtcgtt atcgatgtat tcgggaggga
     5941 aatagagatc gatctcagtc ttgagcttag ctccggggct cttttgaagc tcttcttcca
     6001 acgagacctg tccgcctccc gaacctgaac taatggcttt ttccagcagc gagttgtaat
     6061 agttgaaccc gatcgattgg attatcccgc tctgtttggt acccaggatg gcccccgctc
     6121 ctctgagctc cagatcgcgc agggccacct gaaatcccgc tcccagaaag tcgtattgag
     6181 tgagggcttc cagacggtgt ctggcctcgg tggtagtgcc tcttgggatc agcagatagc
     6241 agtaagcgcg gcggttgctg cgtcccactc tgccacgcat ctgatatagc tgggccaggc
     6301 caaaggtgtc tgcccggtcg atgaggatgg tgttggcgtt ggggatatcg atgccgtttt
     6361 ccacgatggt ggtggagatc agcacctgaa attctttggc gataaaggca tccatgacgt
     6421 cttccaggtg gtgttcgctc atctgggcgt ggccgacggc gaagcgcaca ttgggcatca
     6481 ggttgcgcag ttcgtccgcc acgtgctcga tggtctgcac ccggttgtgg ataaagaaga
     6541 cctggccgcc gcggtcgacc tctctgcgaa tggcatcgcg taccacttcc atatctctgg
     6601 gtgtgatgat ggtgcggatg gggagccgct ctttgggaga agtctgcatc agggagattt
     6661 ccttgagctt ggccaatgcc atattgaggg tgcgggggat aggtgtagcg ctcatgtaga
     6721 gagtgtccac gttgctttgc agcttgcgga gccgctcttt gtgacgcacc ccgaagcggt
     6781 gctcctcatc gatgatcagc agccccagat tggggaagcg gatgtccttg gagagcagcc
     6841 ggtgagttcc gatagcgata tccaccttgc cgctgaggat ttcatctata tctctggcca
     6901 gcagattttt agcccggaag cgactgagca ttgccacccg gatgggatac tgggccagcc
     6961 gttccttaaa gacgcgccag tgctgttccg ccagcagggt cgtgggcacc agcaccgcca
     7021 cctgcgtgcc ggaacataca gccttaaatg ctgctctgat ggccacttcc gtcttgccaa
     7081 agcccacgtc tccacacagc agccgttcca tgggagcggg aagttccata tccgctttca
     7141 cttccagcga agtcctcgcc tggtcaggag tatcctcata gataaaggat tcttccagtt
     7201 cacgctgcca atccgtatct ggtttatgag agaatcccac ccgggcggag cgttcggcat
     7261 atagcctcac gatatcagcc gccaccagtt cgatctgttg ggcggccttg cgtttggtgt
     7321 tttgccagcg ggcgctgcct atgcggtgca aggtagggct ggccccttct tcggccacat
     7381 atttggtgac cattttgagc tgataggtgg gcacatagac gcggtcgtcg ttggcgtatc
     7441 tcagcaccag gcattcggta tcctgagcgt cgagcctgat gatcttgagt ccctcaaaaa
     7501 cccctattcc gtgctcgatg tgcaccacat agtctcctgg cttgagagcc tcataatcca
     7561 caatctcctc tccgggagag aagcggggag tgtaacgctt gcgtttataa cggttaaaga
     7621 tctcgtgatc ggtccagagc accgggccgg tgagcccaat tccaaagcct tcgtgcaaca
     7681 cgccgatatg gtgttcggcc tctgcttcgt aatctgccag caggagtttg gtgcgctggc
     7741 tttggctctg gttgtcgaag agcagaatgc tgcgtcggcc ttcccgggcg ttctctctca
     7801 ggctggaggc caggaggtcg aggtccccac caaacgcagg ctggggctca aagacggtct
     7861 tgagtggctg gttggcgatc cccaggctga attcactctg agacagcaac atggcctttc
     7921 cgggcttgag cagttcaaaa agatgctcct cgccggccag gacgcgctcg ggaggtgtca
     7981 atcgggcttt tgtcttgtgt ttcagctctt tgcgatactg ggtatggacc tgttcctgga
     8041 gttgctcaaa ttcctcccac acatagctga agttgttgaa cagcagcagt ctatcctcgg
     8101 aggcaaagta gtccgcgaag gtggaaagct ctggcaacaa cagaccgtag tggttctcaa
     8161 ttccctcata gaagcctttc tttttgatct gggaggcaaa gggagagtgg ggatcgatgt
     8221 catccagaca aagctcccgg gcggggatca cggtaaagct gtctatctca cctggttcgg
     8281 agcgctgggt attggtggaa aagacgcgca tggagacgat ctcgtcccca aaaaactcca
     8341 ggcggaccgg tttgagcgaa ggcggactga agatatccat aatgccaccc cgacgggaca
     8401 tctgaaacac cttagtcacc tgatactcat gctcatagcc catattgaaa agccgggtcg
     8461 tgagctcttc cggagtgatc tccattcccc ggacgaggga gaatatttgc cgttccagca
     8521 agtggcggga cggcagataa cgggtcaaag ccctaatgga taagctgtaa acggctggag
     8581 caggatcgag gctttgttgc agacagatca tgcgagtggc ccggatcgag tagtgaggtg
     8641 acctctcctc atagggaagt atctcgtaat caggaagata gcgagctttg tcccggccta
     8701 tgaacgccac cagatcatcc cagatgtcct cggcgatgat gtcatcctga gagacgatca
     8761 ggacgttttt gccagtctga ttccacagcc aggccgccat caaagcccgc gcgctttggt
     8821 tcatgtggta ggcttgcacc aactggcctg aatccagggc cgcaagtagc tccatggcaa
     8881 agctttgacg gttcagatca gatagtatga gcggtaggat catggtagtc cttgtggagt
     8941 tgtaaagttc taaagtttta gggtgttagg gttgaaaagg cacagagtgg caaacgggga
     9001 cctttgccat tccgggaggg gtagtgcgtt gagagagtca aggccagcaa ccgttctcaa
     9061 ccatctaaac ttcaaactat caatcgtatc caattagcct cctttacacc acctttacat
     9121 ctcctatgcc tctcctttgc ctaagcatag gaggcgggaa ggtgggaaag gggtgaggag
     9181 ttagcgttca tcagggctgg attcggtgcg cagaaaggtg cctatcacct tgagcaagct
     9241 gcctttgtcg aaagaaacag ccaaggtggc gtcgcgtccg gtaccgttga cgctgagcac
     9301 cttgccgtga ccaaaatcat catgccagac gtcctgaccg atacggtaat gtttgtcctt
     9361 atcggtgatc tgggctctgg tcttgagcct gggctgaggt ctgggagcat aggaagattc
     9421 atctgtgctg ccctgaaaca gagccgcgtc cagatcctgg agaaacatgc tgggctggca
     9481 gtgatcgaaa acgccataga gacggcgctg cctggccagg ctgagcgaaa gattgtcttt
     9541 ggcgcgggtg acgccaacgt aaaagaggcg gcgttcttcc tcgatttctt cgcggctgcc
     9601 catgctcatg cggtggggaa gaagttcctg ttccagtccg acgatgaaca catgactgaa
     9661 ttccaggcct ttggcattgt ggagagtcat caggcgcaag gcttcttcgc catccttcac
     9721 tctgtccaga tcggtctgca gggccacgta gggcaagaag tcggccaata gcggcacgcg
     9781 ctcgttttct ttttggtagc gctcgcagaa ttctgtgacg gaattgacga actccatgag
     9841 gttctccgct ctggcgatct ctttggggtc gttgccctta cggtattggt cgatgagctg
     9901 cagttcgtcg atcacgagac gcaccagatc cggggccgct ttggtcctgg ccgcatctct
     9961 ccaggtatcc agcatgtggc tgaatccagc cagctttttt ttggcagcgg cagccaggtc
    10021 tgagatcgcg tccacggcat gaagagaatc cctgatgccg atgcggaggc tgttggcata
    10081 accgatcaca cggttgacgg tagtctggcc aatgccgcgg ggcggttcgt taatgatcct
    10141 cagcaggctt tcggaatcgg ccggattgga gatcacgttc agataggcca gcaggtctct
    10201 gatctccttg cgttgataga agtgcaggct acccaccagg atgtggggga ttcgatactg
    10261 cataaaggcg ctttcaaaga cgcgggattg cgcgttggtg cgatacagca cggcgatctg
    10321 gcctagcggg ataccctgct cgcgcagtgt cagaatggtc tgggccacct tttccgcctc
    10381 gtcgttttca tcctcgtaga tattcagcct gggtttgagg ccctcgccgc gttcactcca
    10441 gaggtctttg ctgtggcgac ggcggttttt tttgatgatg gcgttggcca gattgaggat
    10501 gctcatggtg ctgcggtagt tctgctccag gcggatcgcc atcacctccg gataatcctt
    10561 ttcaaactcg aggatattgc ggatggtggc accgcggaaa ctgtagatag cctggtcgtc
    10621 atcgcccacc acacagactt tgtgatgccc tcccgcgatc tgacggatga tctcaaactg
    10681 ggcgtggttg gtgtcctgat actcatctat catcacgtgt ttgaactggt tagcatactt
    10741 ggcgcggacc gtgtcgttat cctgcagaag cctggcagtg tagagcagga tgtcgtcaaa
    10801 atccactgcc tgattgaata gcagggcttg ctggtaaagc ttgtagatgt ccaggacccc
    10861 tttgtggaaa ggatccgcgt gactgaggct ggacgcggaa tctgagaggt cttccggtag
    10921 ctgcagacgg tttttgtagc ggctgatcct gctcaggacg cgattgaggg gatacttttt
    10981 gacatcgtag ccgtgttctt tatagatctt tttcaaaagg gatttctggg cgtcgtcatc
    11041 atagatgcta aagcttgagc tgaagggcag ataggcgtgc tcataacgta aaatcctggc
    11101 gcagagggag tggaaagtgc ctacccatag agagcttacc ggaaagccca gcagcttctc
    11161 aaggcgttcc tgcagttccc gcgcggcttt atttgtaaag gtcaccacca ggatgttcca
    11221 gggggctaca cccaggttct tgatcagatg ggcgcaacgg tagatgatgc agcgggtctt
    11281 gccgcttccg gcccccgcca ataccagaag aggactctgg acataggaga ccaccctcag
    11341 ctgggcgtcg ttgagttgcc cctcaaaatc gatgttcatg ttttgttatc agcaaacgag
    11401 gacatttgcg ggcccgctgt caacagctcc acgcgtcata tcggaatcct tacccagacg
    11461 ggcagacagg gcctgccttc cagacgtcct ccggaatact gtttgaaggc ggaaatcttg
    11521 tagtaatact ctccggggac tactgagtgc catggtttgg agccatgact ggcgcgattg
    11581 gtataaaccg tgtccaccac ggcgaaagca gaataaaacc ccagaccacg gtagacataa
    11641 taaccgtctg tggaatattc agggttagcc tgccaggtga ggtccacata cttgctggcg
    11701 gcccctggag cggaagggaa cacctgaaga ttggaaatga tttcggggac gatgatagtg
    11761 gggtcggcgt tggggtcaag agggttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnncttt tccacacctg ggcgttgtat tcctcggtgc cctggatcac atcaaaaatg
    11941 ttgtatatcc acaccagcgc tcctactccc aaaaagatga gggaatactg acggggaccc
    12001 accgcggcgt tgtaatagga ctcgatgtct tccacctggg aggcatccag gtatctgttg
    12061 tagtagtaca tgctgcggtc atagaaatag agtgaggctc ctgccaaaac cagctctgtg
    12121 gccagaaaca ccgaaccttt ggtggattgt ttgttgacaa actgacccca tcccggcgcg
    12181 atggcggatt tgatcacgca gtcacgcagt gaattggctt ccatctcata gagctgcagc
    12241 aagtcctgcc gttccaacag gtcctgccgg taggcgttcc gctccagata tgtctgccag
    12301 ccgtatttgg tgctgtccga aaaagcgttg atatcaaagg actgagccca cccatactgg
    12361 catccgagca ggaggaggaa gagtaccaga cgacggctca tttcattagc tcctcggata
    12421 tcttgttaag gggtataatc ttgcggtctt taaggctgac ctgccatacg tacaggaagg
    12481 tctcctgagg ggtcatcagg tcgtagcggg cttcatattt atattggccg gtggagagca
    12541 gaaacttcca gccggaatcg cggatttcgc tgttcttata gcgtcccaga agctgctttt
    12601 tctgttgggt gatgcggttg ttcagcaaac cgttgcggta ttcattgatg atcttgcggg
    12661 caaacgcgag gggattgcga tccgcctcaa tagtgttttg catgatggag atgcgggtcc
    12721 tggtctcggg attgctgtcc aggccgttgg cctgttgata aagcttgagg gcgtctgcca
    12781 cttttccact ggcttcggtg agctcggcct gcctgaaaag ctgagcggcc cgggcgatat
    12841 tctcctgggt gattctgact ctttcgatag cctgccgggc gggcgggtaa tccgggatga
    12901 tctcaaagac cctctggtaa tgagtgagcg cgttttcaaa atcgcgctgg gccagaaacg
    12961 cgtctccctt cttgataaac agatcgacga tctggcgcag acgttcctcg atctggggcc
    13021 tttgctccgg aatatactgc agggcgatgc gcaaaaagcg gtccgcctcc agatagtttt
    13081 cctgatccag atacagctcg gcctgaccgc gataggtgga ccctataagc gcgttgatct
    13141 cttccgtcct gatgatggga tacttggcca gctcaaaata gaccgccaaa gcctcgttga
    13201 aatatcccgc gttggccaga cgctctccat aagcgatctt attcggcaca atcctttcca
    13261 tggacttgcg ggccgccact tcatacgaat ttcccgggta ggtgtcaaag aggttcatgt
    13321 aatcatgcca ggcagcgtcg gcatcgccaa tctggtccag atagatcacg atgcggcggt
    13381 tgatcatttc cggcaccagg tcggaagtgg ggatctctct gaggatggaa ttcaaataga
    13441 gcagttgagt ctcaaagtca ccaacggtcg cggcttccag gagtctggat ttgtagattt
    13501 tggccagttc aaaatcggcc tgggatgagt tggaaagctt aaaaaaccgg atggccagat
    13561 cgatcgagtt tcgctggacg gcgatcaagc ccagctggta atagctgttt gaccttacca
    13621 gttccgcgcg tgtccttagg gccccgttgt tggctttaag gataaattca tcgctttcct
    13681 ggatggcggc ggcgtagcgt ttctggccgt aatgctcctc ggcgctgcgt aaacgattga
    13741 tctcacaggc gctgatcgtg acaagaatga tggccagcag ggccgcggaa tacctcatag
    13801 ctctatgtga tacccgatat cttccatagc cagctcaaaa ttgtcggcct ggtggtgctg
    13861 cagccatagc tccagttcct ccgaaagccc gtgagtgagc agcactctgg ggataccgtt
    13921 gtccgccagc ttcaagatct gctccagggg tggatgcatg gcgtccacga tcgcggtgtg
    13981 acatccctgt aatagagacg cgacgcaatc ggtgctttgg atatcggccg tatagacgag
    14041 gtctcctccc ggttctccga ttctgaaagc ccaggagcgc atctggtttg gcaggtccag
    14101 ggccgtcacc agctcttcat agccccaaag atgatccgtc tgcatggcgt gtatctgggg
    14161 gaaatgatcc cggatggtgt catttgagag gatatgcagc ttgaaaccaa gcttttcgag
    14221 gaaggtgtac tgatactgca ataatgactc aaactcggtc tctcgctccg ggaggaagag
    14281 gaaaaggtct ttgctgcgct tctgcagata gagcatctgg atcagcatga agatgccgga
    14341 aatgtggtcc gggtgaaagt gactgatgaa aacggcgtcc agcacctcac cgctgagtcc
    14401 atgccgcagc agctgacggc tgcagccttc cccgcagtca aacaggaaat gtctatcacc
    14461 gtggttcacg taaatactac tgagacattt gtctggaagc ggaaggcctg gtcccgcgcc
    14521 aaggatgata aagttcattt gatctttcca gttttcttct gattttctgg gatcaccacg
    14581 ggaacctggt caagctttct gccaaagaac aagaagaaag aagcgaacag gatagtggtg
    14641 attaatagca gctctctgga cccgatggta aacacgataa tcgaagtgag aacgatcagg
    14701 acgttgatgc tgtatatgat gaaagcagtt acctttatgg accccagagc gttgcggatg
    14761 cggtgagtgg agtggtcttg ccctccctgc atcacatggc gtccgtctct gccccgggtg
    14821 tagctcacga gggcgatatc gaatatggca taactgagca ggagcaccgg catcaggaaa
    14881 aacatcttgt cgttgtagcg gttgccggcc agtcgagcca tcaggatccc catggaggag
    14941 agaaaatagc cgatgaacat cgatccggcg tctcccaaaa agatgcgggc gggattgaaa
    15001 ttatagggta aaaagccaaa gacggaaccg gcaaaactga gtgagatcaa cccgatcagt
    15061 gccatggatt cctgattggt ctgagtggtg gtagtgaggc tgaaggcaaa gaaccccaag
    15121 cccaaaatgc cggccattcc actcagaatg ccgtccatgt tgtccaggaa gttgagggcg
    15181 ttcatcaggc ccaccatcca caacaccatc aggggtatgg agatatagat gctcccaaac
    15241 atcaggccca gctccgtgag cgtgaagttg ctgaggataa agatgaagct taccacgatc
    15301 tgtcccagca tcttgatcag gggctgcatg gcgcgtttgt catcccacag gccgatcagc
    15361 atgattagca gcgagccggc caggtaaccc atcatgggct tgtcaaaata gcggccggga
    15421 gaaatggcga cgtcgtagag gcaaagcagg aaaaaaccta tgaacacact gaggcccccc
    15481 atcagaggtg tcaccttggg gtggtctttg cgttcgttgg gtttatccac cagattggtg
    15541 gcgcgggcca gcctcatgat aaagggggtg aggccataga ccagcatcca ggacattatg
    15601 aacacgctca ggtattcata tatccggttt tccatattcg tctccactat cagtaataat
    15661 catgctaagc tcttcaaggc ctcatatctg tcaattacaa attgcgcttt gaagtccggg
    15721 ctggtagttt tgcttcaggt agatagacct ctgtccgcgg agcggacaaa ctccacgaag
    15781 gtctgctgtt ctggcaggag gtctgtcctg gagtccgcaa ccgcgagggc ttgagaagtg
    15841 ttgaggccct tgcggtaaaa gaggttgtac atctccttga tggcggccac gctctggttg
    15901 gaaaagcctt tgcgcgtgag gcccacgctg ttcaggccca ccgtcttgta aggatttccc
    15961 tggccccggg tgaagggagc gatgtctttt ttgatggcgg aagcgcctcc cacaaaggca
    16021 tgggtgccga tatggacaaa ctgatggatg gccgtcgcgc ctccaatcgt ggcatagtcg
    16081 tggataatga cgtgtccggc cagctggaca atattggcaa taatgcaaaa agacccgatc
    16141 tggcagttgt gagcgatatg gacgtactcc atgaggaggt tgtcgtttcc cacgatggta
    16201 tcttcgccgg cgtggttgga gcggttgatg gtcacaaact cccggatggt gttgcgggaa
    16261 cccactctga ggcaggtgga ttctcctgaa aacttgaggt cctgagggtc ggtgcccaaa
    16321 acggcgtgac tgaagatgcg gttgtcgtct ccgatcgtgg tgtcgccatc gatgatcaca
    16381 aagggggcca gttgattgcg ctcgcccagt ttcacctggg ggccgatcac gcagtgaggg
    16441 ccgatcacgc agtcgttgcc gatcacggca tcgggatgca caatagcggt ggggtggatg
    16501 gtgctcattt gttcttttcc tgcagcatgg ccatgaagtc tccttcggcc actttttcgt
    16561 taccgacaaa agcttctccg tgcatcttgg agagcgagcg cttgagtgaa atcactttca
    16621 tctcgtagcg cagcgtatct cctggcagca cgggtttgcg gaatttgacg ttgtctatgg
    16681 aggcaaaata ggccacgtag tctctggggt tctcaagctg gttgagcagc atgattcctc
    16741 cggcctgggc cattccttcc aaaatcaaca ctcccggcat gatgggatgg tcgggaaagt
    16801 ggccctggaa gaaaggctcg ttgatggtca cgtttttgat gccagtaatg gattcgttgg
    16861 gcacgaaatc gatgatcttg tccagcagca aaaatggata gcgatgcggg atgatgcgca
    16921 tgatggcatt gatgtcgaag accgcttcct ggttgcgttt tttctggtac atcatctgga
    16981 gctcgttttt cttctgaatc tggcgcagct ttttcaccag ctccacattg gtcttgtgac
    17041 cgctgcgggc ggccagaatg tgaccccgga tcggcattcc caaaagcgcg atatctccca
    17101 gcaggtccac cactttatgg cggacaaact cgttgtaata gcgcaacttg ttgttgttga
    17161 ggatgccttc ttctgagacc tggacgggct tgtgatagtt gaacacttgc tgcaggtggg
    17221 taagttcctc ctcgctcatg ttgggttcgg cgattacgag agcgttgtcc agagagccgc
    17281 ctttgatcaa ccccttttcc ttcagataga gaatttcgtt gataaaacag aaggtgcggg
    17341 ccccggcaaa ttcccgttca taatagtcca ggctgggcag ccaggtgtat tgggtgccga
    17401 ggttgggatg cttgtaatcg atcatgaagg tcactttgag ctcgttggag ggcacgatca
    17461 cgatatcgac gttctcttcc ggagcggaga aactgatggg agcgtccagc tcaaagaaga
    17521 cgcgttcgga atcctgctgc accaggccag cctgcctcaa aagattcaga aacacgatgg
    17581 gagagccatc cgccacggga gcttctggac cgtcgatctc gatgcggaca ttgtctatat
    17641 tgaggccctt gatagcggat agaacgtgtt cgatggtacc taccacggct ccgttgatcc
    17701 cgatcgtggt cccccgggaa atatccacca cgtgatcgat atcggcgggg atttctggtt
    17761 tgtctggcaa atccaccctg ataaagacaa ttccgtcatc tttgccggcc ggcttgaagc
    17821 ggatggtgga tatctcgcca ctgtgcagac cgatcccagt gtaggaaacc ccgtgctgaa
    17881 tagtgtgttt atactctgtc atatatctct ctcagacagg atagctctgc cttccagatc
    17941 attcctgttt gttttttaaa taaaaccggt agatatccgg cagatgcttt tgggcggcca
    18001 tgatgcgttt catctggtta gcttccgtgg ccggggaacc aaagtatttg gaatctgccg
    18061 ggatgtgatt ggtgatccca gcctgagccc caaccatagc tctgtcgccg atggtgagat
    18121 gtcccgcggc tcctacctgg cctgccagat agacgtaatc acccaccacc gtgctgcctg
    18181 ccagccccac cgcggcacag agaatgcact gccggcctat cacacaatta tggccgatct
    18241 gtacgaggtt gtccagctta gtgccatctc cgataacggt cgagcctaaa gtagcacggt
    18301 cgatacagga attggcgccg atctcgacat aatctccgag gatgacattg ccgatctgag
    18361 ggatcttgcg ctgcactccc tccaggaggg caaagccaaa accgtcggcc ccgatcaccg
    18421 ctccagaatg gatgatcacc tcgcgcccga tcacgcagtc ttcatagatc accacgttgg
    18481 ggtaaaggat gcagccatcg cctattacga cattgtccgc gatcacgcag ttggcgtcga
    18541 tcctgcagtt tgatccgatc ctggcgttgg ccccgatcct cacaccagca tctatcctgg
    18601 tggtttcgga taccgtggcg ctgggatcga tcaccgcgct gggatgaatg ccggcaggct
    18661 ttttgggggc catcgagagc cagtagctca gaagcgtcat catcgtgata tagggcttcg
    18721 gggtgatcaa caggttgcgg ccttccagtt tgagcgcaaa ctcaggagtg gtgatgatca
    18781 ggctggcctg gctggcctgg gtggtttcca gcagcttttc ctgctccaga aacaccacac
    18841 tgcccgggcc ggcatcctca ggagtggaaa cactgtctat cctcagctcc ggatctccct
    18901 gatagctggc ctgcgggagc atattgagct gcgcggttcc caggctgggg ctgagttttt
    18961 tcatcttagg gcttgatcgg aggtttggtc tcgttggggt tggtgacgtc tcccggcatg
    19021 gggtccttga attcaggttc aggcttggtc tcatcaggct tgagctcggt gtccgcttcc
    19081 gttccgggag ctatggtgtc tttattgatc tccagcagca cgatatcggt gatatcgatg
    19141 gatggaatgg catagaggat agtgggatag ctgctgtcga agatcattgt gtacttctcg
    19201 tcctgggcta ccttgacgat cacttcctgg atcttgagag tgagaggatc aatgagctcg
    19261 cgataacgct gttcagcctt tccgccttca ccaaagtatt cctgtaaaag gcgtccggcc
    19321 tccaccttcc tggcgtctat cttggccaga gcgtcacgtt tggccgcttc attcttggtg
    19381 agcttgtcga tctcg
//
LOCUS       DAOSFN010000028        19329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2290_length_19329_cov_9.535851, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000028 DAOSFN010000000
VERSION     DAOSFN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 19329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19329
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2290_length_19329_cov_9.535851"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(491..1846)
                     /locus_tag="PLO35_03225"
     CDS             complement(491..1846)
                     /locus_tag="PLO35_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOH78707.1"
                     /translation="MKILIIDDERNICYTLRGILEDEGHEVFSAYNARSGLALFEETE
                     PDAVILDVKLPDGNGLDILAKIKTVVPELPVIMISGNSNISEAVKAIKIGAFDFLEKP
                     LSLPKVKITVSKAIQFSDLAFQMRSLREQNESAWEMIGDSAPMQALRQIIERVARSNA
                     KILIQGESGTGKELVARLIHTHSDRSGRAFVKFNSAAIPKELVESELFGYERGAFTGA
                     EKRKKGKLEEADGGSLFLDEIGDMDLAAQAKILRVIQEGEFERVGGNATQRIDVRVIA
                     ASNKDLPALVASGLFREDLFYRLNVVPVRTPALRERKEDIPMLIEHFSRLVAGELKVR
                     PKTFSKDAVALLKSPDYPGNVRELRNIVERIYLLCEAKDLQAEDIASHLIGTSSGNGS
                     SDSGFWSAKSTYADKKHEFEHRFLSTQLQLYGNSVTRTAEALGLAQSNLSRKLHELGI
                     L"
     gene            complement(1843..3003)
                     /locus_tag="PLO35_03230"
     CDS             complement(1843..3003)
                     /locus_tag="PLO35_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOH78708.1"
                     /translation="MKFIYHPGLRSWIIMLFVVVSIGGLVLLNSFLQNQQRQIEKDLA
                     KLNFAEELREFHFTQPEDSLRARQIMDTFSQAKGVMSFTLSRSRMYSSFFLLLIIVAS
                     IVVFMILLSRIINPLRELQKATDEVRRGNFSVHLPETGIHEIKLLKSSFNTMGRELEA
                     IQKRLLVAEKEMIWKDLSRVLAHEIKNPLTPIQLAIQRLEERYQDDPETVIKILPDSI
                     AVIQQEIENLRLLAQDFSNYARVTQARKELFDPAQSIEEICRSYRDNRKLDLDLEPDH
                     QINFDPTHFYQIITNILQNAMDASEPDKPIAISLTHERDFVIISIRDQGSGIDAEDLH
                     RIFEPYFSRKSKGTGLGLALVKKLCEINGAIPRVKSKLNEGSEFILIIEEAP"
     gene            complement(3016..3552)
                     /locus_tag="PLO35_03235"
     CDS             complement(3016..3552)
                     /locus_tag="PLO35_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78709.1"
                     /translation="MSAKVMAGLVSLSMFLFSTYTGNDPQLSSIRATMGRSYLQLNTR
                     LEHAFDNDFPELFKSGTTIPVHFRLEIKSKKQVHWSKQYTNSSRYDSSTGFWSVSQSA
                     ASRSVKFSSYQQMIAAVAELDCSIPYESNWGDVNVKVEAWLPTVNFDKIDRKVNLMVL
                     WNYQRPVAKASYNLRKPL"
     gene            3783..4313
                     /locus_tag="PLO35_03240"
     CDS             3783..4313
                     /locus_tag="PLO35_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78710.1"
                     /translation="MRKYQVAVLVMLILGLAVSVMAQNWNNQSSTYYTISIPSTWTVA
                     METDSLFVATMQGSALTPLTVIVGVDPISEEEKDMSLEELAAKSLEEGNAEFIAMGLE
                     DAIDVVESGEVKFNSYTAYHHLVKIAVMGMNITTDSYIFTKDNQSIQLMIMGDDTDIQ
                     AQAPQIASIKKSFKLK"
     gene            4323..5189
                     /locus_tag="PLO35_03245"
     CDS             4323..5189
                     /locus_tag="PLO35_03245"
                     /inference="COORDINATES: protein motif:HMM:NF020468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HOH78711.1"
                     /translation="MRSPVLTILSLLLLCGSVFAIDLSSPAAYFDTFLKTRADLSGKE
                     TVFHWTGKVYGMVPGERRTELFAFEGFSVARLEKAETGYLLLTREAAFFKDPRTGEIL
                     GTWRNPYLKNAETQVVHIWNDPVNQDFGFSAEEMPLISKFLPSEDLGSELVFYMDLFP
                     LYESPLPRKEYSQFSQSDIYQAAEFFQFYVPKSAIADSSLTSLPCNISWTRISPWMPF
                     MRMGDRKGNLVFACRGSKLEGGFEALPAQIRNYVMEKAPRFATAPDEYSEPNETSWTY
                     FKKLMDAGVIKP"
     gene            5266..6747
                     /locus_tag="PLO35_03250"
     CDS             5266..6747
                     /locus_tag="PLO35_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78712.1"
                     /translation="MKKCVVTAILLLGFSFALAQFSSPWLNIRHSSRNIDGNLQFRWE
                     SADAMLGPAQLWARLGTGDWQTQDSSLLEGVSYEATPAYTWGQRLRVRLRNEMEMEGV
                     PAAMMQAAWMDDDTFPQAQGKLALIGPDPVGDSLMIQAPMLDLGNTWMGTTDQKLYST
                     ISNQSGSFPTMNSLTSYNAWITTIANPEAIADSVVYAMVYTFNIVGVISSGLYKLGID
                     ETMTPTFARIGSIQTSVSGGKLNMACNLSDLTSDPSFGTWPNLSNALLFSSFSMRLNI
                     DLSTMEPEMLIGDYSMPAIVFFEDIQYLHSQNTLPDVTGLDVNGLIVSCQYQDAEGDF
                     PLLCQFVVEDGPTLQMQPGSYDFLTGVTYSAMLSSPIQNGVLQVSDNLLYVQEYKWSG
                     VAVEDPSLVPVAMSCVIPNPVKAGQTVAIRFDGLKTGKLRVEIFNLRGQKLSSLDHDP
                     QGSHAACINWNGLAGTQTPAPGVYLLRATQGSQVLTRKFSVIY"
     gene            6767..7777
                     /gene="obgE"
                     /locus_tag="PLO35_03255"
     CDS             6767..7777
                     /gene="obgE"
                     /locus_tag="PLO35_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOH78713.1"
                     /translation="MFIDHAKLKVRAGNGGDGAVSFRREKFVPKGGPDGGDGGRGGDI
                     IALGDENVNTLLDYRFSKLYAAENGKHGSGARKTGSSGEHVYLRMPLGTEIFRADESG
                     RKVKLGEITQHGEELVIAKGGHGGKGNSNFATPTNQAPRHATPGRITHEMELELVLKL
                     MADVGLVGFPNAGKSTLLSVLSAARPKIADYEFTTLEPMLGVVRVGDYRSFVMADIPG
                     IIEGAHMGKGLGHQFLQHIQRTSMLLFLIEVTSSDPVAAYQTLRAELYLYDKFMDKKP
                     FLVVVSKLDLIPEEERDEILGVIDSQFRDKFGVGVMAISSISQSNLETLKYRVFELLS
                     KA"
     gene            7774..8871
                     /gene="dprA"
                     /locus_tag="PLO35_03260"
     CDS             7774..8871
                     /gene="dprA"
                     /locus_tag="PLO35_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOH78714.1"
                     /translation="MTDPIKLRAWLCLKTAPGLRGKDIFRLLEQWPQPQEFIGNPEHP
                     VYQSKDWDEQTLRHMAEGKLPPNYPQVSKLVELYEIGLLCYSDPDYPLGLKEIYSPPV
                     ILYYRGDLIKALKQICLAVVGTRKPTAYGREMCAKTIALACRNDVTIISGLAMGIDTV
                     AHLTALEQHSGTIAVLAAGVDSIYPPQNRELAKQIVAHGALVSEYEPGSRLEKWNFPA
                     RNRIVSALAQAVYVVEGPLSSGALLTAKFAIEQDRELMALPGEISHPNAQGPNYLIKS
                     GARLVSCPEDVLEALGIETQPDQQLQIMPELSESEQGVWDIFQSEQRELSFDDLLLKT
                     GLSFGKLSIILLNLELKGCIGKASGNSFVTR"
     gene            8903..11416
                     /locus_tag="PLO35_03265"
     CDS             8903..11416
                     /locus_tag="PLO35_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOH78715.1"
                     /translation="MNRRLIILIAILALSVGLGANLPAAYTAKGNPALYDSLKVLAHQ
                     NLSLLDKPAQQAYTDLLRRYDDILMNYLIAYETDTNLAMADPASLESNYAHILPLLTQ
                     SKHSFSPEFFLSYVARQTVSDERIQAYRKAFLDDGLRVLMTKVSDEVELYREVCKWCL
                     ERLTFQPTSGRDQSPLDITRKSYLGRCEEMQILFVAAARTVGLPARPASTPWWAHSDN
                     NHAWAEVWLEGAWHYTGDMDSAYYPDQTWFSGMIDKTVLILADGSLASPNDEVLSSGR
                     YDKLINSTSNYAKERTRMLEISVTDDKGKAVPEALLGILVYNWGALRPLTFLNADAQG
                     RFKLSVGRGAFYVSAFKDGLSALKLVPSTEDKLMELALVLSAKTLPAQNELLAYPSNP
                     FEWKQAPQSWKDDVSAVKERIARREQVFASRAIPAIWEPYDSLFWNVASACRGNDLEF
                     QLFASRHRPVDAEFLSFLLADDPKLLWQMDATQFEALYRFFRLHASDRLSSEALHSLI
                     SPSVYYEELPRPWLTKRDGYQLYPEDFIQPGKTPLKKLGQISKWLNKRYKTNPDKALS
                     GLLPLHIATGRKYLTAFQKRILAVNLARANGVPADFTRIPNVISVMLEGEWQYYDIQK
                     GKLWSGGQEGESKDFDLYVNIMDENGFPLEIDASQLVLTRWEDGNFYSLNHRFLPSGK
                     GRYTQQVPRGSYYLQMGYRVSDSLTGFLMEPVMAEKLDSLALILQPKSYPRSWNALDP
                     AIAAWLEPADLQNHAIILIGNIDHENSIRIAEKLKETDKAFLWLGYNEATDAPSSYRV
                     SKTWQEAVRADQRNAVRTITIIKTDSGWQMYEGLWDKMP"
     gene            complement(11665..13791)
                     /locus_tag="PLO35_03270"
     CDS             complement(11665..13791)
                     /locus_tag="PLO35_03270"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH78716.1"
                     /translation="MRAKSLSVLLLFLCGSLSAVYVNGYIQDENSRPIEQALVELGQV
                     WTLSDATGYFSLQGEGDSLRVSRLGFQPYAVGIKSGTLKISLRARPVELPTVRVIESY
                     SSFVNSAADLLSVPIDPDRHYFSATQMLGEASGTSSSDTPLRGEVQSIRLLGNLPRHS
                     LILLDGIPLNAEGESYDLSLLDPQNIEKIEIIKNNASVYGGGAAIGGVVMITSRSGRL
                     AQAPQYRAGIEVGSFGYAEARLSFKGNLGALAYRLAMSKFNADNDFKYEPREWWGLQG
                     DQTRSNNAKRQNSLSGSISTRLGQSLLILSSDLEEFHRQLPGTVNFSEIYRNAWLKGY
                     ANRNRISLEAPLAGLNSRAQLWYNQDRTLYDNTSAPLPVFISRYRQGLWNAGIKLGLE
                     KSEGLIRAGLGAEAASTSYLNENLLNAGSPLRYQAEQAGFTVRAGMVKDGDLLGGSAY
                     IVGRYDLTATENVASWRAETELRCKSWAETSLGATWGTSFALPSPYDLYWKGDSQAIG
                     NPDLNSERSRGGQFWASGKAYGFNLRGAVHLNQVTDLIQWRQVQMFGNAWKPFNIGKA
                     ELRNLEVEAGWDGLKWLNISAEALFTDARDVSSLAFAAAPQLMYTPRRQLSATARIKL
                     NHLSIWATYSHTGKQFTTPDNLSAPVDAYELLDIGLEGSFEWQGWQLYPHLRAHNVLN
                     QRYEVYPYVPQPGISIFGGVEVRASL"
     gene            complement(13781..14521)
                     /locus_tag="PLO35_03275"
     CDS             complement(13781..14521)
                     /locus_tag="PLO35_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH78717.1"
                     /translation="MILRGLDISLREDEFAVLAGPNGAGKSTLLYALLGFLRLEAGRI
                     ELFGRELRNYKRSELARLIAFVPQESLFQFDHSVFDIVLMGRYPYLGLMQSWTSQDRA
                     AVGKVLQSLGLEAMAERFYSELSGGEKQRVLIARALAQDTKYIFLDESLSQLDINHQI
                     ECMELLRGINRKEGKGILLISHNLNLAANYADKLIFLKDGTGLGTGKPAEMMDPLKLE
                     ALFGMRLSTLINPSSGIPNLVYPGPDAR"
     assembly_gap    14638..14737
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15111..15533
                     /locus_tag="PLO35_03280"
     CDS             15111..15533
                     /locus_tag="PLO35_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOH78718.1"
                     /translation="MGVNTRTEYALRALLEMSGNAEEPISAQRICLRQQLPKKYVEHL
                     LSALKVAGLVSSSAGSRGGYTLTRKADQISLLDVMEAVDDHSLELECGENRGEHCLGD
                     DCQLSPFFHQISQEQRSLFASYSLATIASFFEPRGARK"
     gene            15530..16720
                     /locus_tag="PLO35_03285"
     CDS             15530..16720
                     /locus_tag="PLO35_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOH78719.1"
                     /translation="MSQRIYMDNCVTSPLAPEVFEAMKPYFSEQFWFPGTFVSTGEAT
                     NSAIEGFRKTVADALNAQSSEITFTSGGTLANNIALKGLTLANSDKGRHIIVSVVDYP
                     DLLTNAAYFESMGFELSYLEPDAEGMVSADKLKAALRPDTILFMSTMVNHVVGTIQPI
                     AEYSQVLAAADHKIWFHVDAGQAFGKLPLDVKALNVDTLSVSAHKLHGPQGIGALYVK
                     KGTKLSPYIHGVKRFDSLQPGGLSLAAIAGFARAVELVFQDLDGNIRKLRELSDYLLE
                     RLKGQIDHIELNGPRGAGRACHNINVSIDYIEGEAITMMLDMHGITVATGSACASQGL
                     KPNYVLMAMGKNHVQSHGSMKFTLSRYNTREQIDFTVARLKEISATLRERSPLYTAIK
                     DQER"
     gene            16721..17347
                     /locus_tag="PLO35_03290"
     CDS             16721..17347
                     /locus_tag="PLO35_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOH78720.1"
                     /translation="MQYSEKVLQHFMHPKNVGKMENPDSTATEGSPACGDQVTVYLKV
                     DDASHTIEDISFLSYGCASNIATASIITEMAKGKTLEEAKQITWRDAMEALDGLPPVK
                     AHCSVLAADTLQTAISNYEIAHGLKEVPNFSKDTIIEELKKIIYPKVGEDIITLKMVK
                     YVGWDNGEVLIDLNLLSFDQYRDNVAEEIHEHLEKYPEVKNIVINFPS"
     assembly_gap    17373..17472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17566..17844
                     /locus_tag="PLO35_03295"
     CDS             17566..17844
                     /locus_tag="PLO35_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78721.1"
                     /translation="MVSRSLTSLFWEYQISEQELQDCLDKNDLNDPITVSIYNRLLLS
                     TPDWYSLLDMLSCEQLKAALSPTVIKTIHSKALRERYSFAASRLFPEK"
     gene            17846..18526
                     /locus_tag="PLO35_03300"
     CDS             17846..18526
                     /locus_tag="PLO35_03300"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOH78722.1"
                     /translation="MDRSLYHNALYPLQDTVLSLVVELDLPFYLTGGTALSRHYLNHR
                     YSDDLDLFVNSHPDFRGLVDAIEAGFKDKQLTYQAITRSADFVRLQVSGTDDTVLKVD
                     LVNDLVCHIGEFERSPQLGLVDNVFNILSNKISALPRLAIKDFADIVFIARKYTFNWK
                     VLIGEAFQKDEWVNPLDLARYFAEVPPNRFELIQWVEPVNVSEIILSCQKIADEIILG
                     ADNSLAQK"
     gene            18735..19157
                     /locus_tag="PLO35_03305"
     CDS             18735..19157
                     /locus_tag="PLO35_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78723.1"
                     /translation="MYQPDMVPADPQYRRQVLILLLIIALGLGFLLYSLQPYLQANID
                     TGTPEQTLQLLKWMLIALGIVPLGFAIYMLVIAIKVYRQGIFPPEGTKVLRDTPILFD
                     QDARRRANILVVLAALLMFMSDLLILMAFYLPKLLTRL"
BASE COUNT         4519 a         5280 c         5018 g         4312 t
ORIGIN      
        1 tatagatatt gatacggctt tatatgccat cttcatacga ggctgtagct cccttattcg
       61 tatcccgagt tgccatcagg accatcccct acccatcccg aaccagattg ggggtgcgtt
      121 gggggagctt tggacaggct tccgttatgc ggttaagtgc catccaggat gaggaattgt
      181 ttgcggacct gccgtatcct tatctttgct gcttagccgc gctggagata ccgtgcgctc
      241 tccgccgaaa atccccacag agcgcacggc agctcaacgg caagtaagca ggaaggatgg
      301 aggccatgcc acaggcgctg atcgcgcctt agcaaacgag gacgtttgcc gttccatgat
      361 cgcctgtcaa tccaccctcc gtagctctct ctgtttcctc agtagctctg tagtgaaaaa
      421 gaatcctctg tagctcagga gctaaaacac tcgccggatt ggaatccgta gatacataac
      481 gggcgtcaag ctatagaatc cccagctcgt ggagttttct ggacaggttg ctttgggcca
      541 gccccaaggc ctcagcggtg cgggtaacgc tgttgccata cagctgtaat tgcgtgctca
      601 ggaatctgtg ttcaaactcg tgtttcttat cggcataggt ggatttggcg ctccaaaacc
      661 cgctgtcgga agagccgttg ccactgctgg tcccaatcag gtgagacgcg atgtcctccg
      721 cctggagatc ttttgcctcg cagagcaggt agatgcgttc cacgatgttg cgcagttctc
      781 tcacgttgcc tggatagtcg ggtgacttca gaagcgcgac agcatccttg ctgaaggttt
      841 tgggcctcac tttcagttca cccgccacga gccgtgaaaa gtgttctatc agcatgggga
      901 tgtcttcttt gcgttctctg agggcaggag tgcggacggg caccacattg aggcggtaaa
      961 acaggtcctc gcgaaagagg ccacttgcca ccaaagccgg aaggtctttg ttggaggcgg
     1021 cgatcacacg cacgtcgatc cgctgggtgg cattgccccc gactctctca aattcacctt
     1081 cctggatcac tctgaggatt ttggcctggg ccgcgaggtc catatcgcca atctcatcga
     1141 ggaaaagact accgccgtcg gcttcttcca gtttgccttt tttgcgtttt tcagctccgg
     1201 taaaggcacc tctttcatag ccgaatagct cgctctccac cagttctttg gggatggcgg
     1261 cggaattgaa ttttacaaag gcgcgaccag atctgtcgct gtgagtgtgg atcagacggg
     1321 ccaccagttc tttgccggta ccgctttcac cctggataag gattttggcg ttgctgcggg
     1381 ccacccgctc gatgatttga cgcagggcct gcatgggcgc gctgtcacca atcatttccc
     1441 aagcgctttc attctgctct ctcaggctgc gcatctgaaa cgccaaatca ctgaactgta
     1501 tggctttgga gacggtgatc ttgaccttgg gcagagagag gggtttttcc aaaaagtcaa
     1561 aagcgccgat cttgatggcc ttaactgcct cagagatgtt gctgtttccc gagatcatga
     1621 tcactggaag ttccggcacc actgttttga tctttgccag gatgtccaga ccgttgccat
     1681 ccggcaattt gacgtccagg attacggcgt ccggctcggt ttcttcaaac aaggcgagcc
     1741 cggacctggc gttgtaagcg ctgaagactt cgtggccctc atcttccagg atgccgcgca
     1801 gggtgtagca gatgttgcgt tcgtcatcga tgatcaggat cttcatgggg cctcttcgat
     1861 gatcaggata aactcactgc cttcattcag cttgcttttc acgcgtggaa tggccccgtt
     1921 gatctcgcag agcttcttta ccagggccag cccgagcccg gtgcccttgc tcttcctgga
     1981 aaaatagggc tcaaagatgc gatgcaggtc ttccgcgtcg atcccgctgc cctgatcgcg
     2041 tatagagatg atcacaaaat cgcgttcatg ggtaaggctg atggcgatgg gcttgtccgg
     2101 ttcggaagcg tccatggcgt tttggaggat gttggtgatg atctgataga agtgagtggg
     2161 atcgaaattg atctgatgat cgggctccaa atccaggtcc agcttgcggt tgtcgcgata
     2221 tgagcggcaa atctcttcga tgctctgagc ggggtcaaag agctcttttc tggcctgagt
     2281 gacgcgggca taattggaaa aatcctgagc cagcagcctg agattttcga tctcctgctg
     2341 gatgacagca atgctgtccg gcaggatttt gatcacagtt tccggatcgt cctgatagcg
     2401 ctcttccaag cgttgaatgg caagctggat gggagtgagc gggtttttga tctcatgggc
     2461 aagaacgcgg gaaagatcct tccagatcat ctccttttcg gccaccagca gacgtttctg
     2521 gatggcttcc aactcgcggc ccatggtgtt gaaagagctc ttgagcagct tgatctcgtg
     2581 gatgcccgtc tcgggaagat gcacactgaa attgccccgc cgcacctcat cggtggcttt
     2641 ctgaagctcc cgcagaggat tgatgatacg ggacagcagg atcatgaaga ccacgatcga
     2701 ggccacgatg atcaacagca gaaagaagga agaatacatc ctggagcggc tgagagtgaa
     2761 gctcatcaca cctttggcct ggctgaaggt atccatgatc tgtctggccc gcaaagaatc
     2821 ttccggctgc gtaaagtgaa actccctaag ctcttcagcg aagttcaact tggccaggtc
     2881 tttctcgatc tggcgctgct gattttgcag aaaactattc agtaacacga gcccgccgat
     2941 ggatacgacc acaaacagca tgatgatcca gctacgcaga ccggggtggt agataaattt
     3001 cataaacgct attccttaca gaggcttgcg gaggttgtaa ctggctttgg ccaccgggcg
     3061 ctggtaattc cacaacacca tcaggttgac tttgcggtct atcttgtcaa agttgacggt
     3121 gggcagccag gcttccacct tgacgtttac atcaccccag ttgctttcat agggtatcga
     3181 gcaatccagc tcggctaccg cggcgatcat ttgctgatag gaagagaatt tcacggagcg
     3241 ggacgcggca ctctggctga cgctccaaaa gcctgtggag ctgtcgtagc ggctggaatt
     3301 ggtatactgc ttgctccagt ggacttgttt tttggattta atctccagcc tgaagtgtac
     3361 cgggatggtg gtgccggatt tgaacaactc cgggaaatcg ttgtcgaaag catgctcgag
     3421 gcgggtgttt agctgcaggt agctgcgtcc catcgtggcc cggatcgatg acagctgagg
     3481 gtcgtttcca gtataggtgg aaaacagaaa catcgatagc gagaccagcc ctgccatcac
     3541 tttcgcgctc aaagtctcca gcgttacgat acctcacaac cgtttcgttc cagtccagaa
     3601 ctggcgggat ttttgtcaag tccggtttga gggtcactgt aagatatctg gttaaagagt
     3661 ggctatggag gagttaggac agagaattca tagccaatcc gcgggtgggg ataaatccag
     3721 cttgacatat tcaggggatg aggattgctg ggataaatat ttatgttctg ttaggaggaa
     3781 caatgagaaa gtatcaagta gcggtattag tcatgctgat cctgggattg gctgtatcgg
     3841 ttatggccca aaactggaac aatcagagct ccacatatta cactatcagc atccccagca
     3901 cctggacggt tgccatggag acggacagct tgtttgtggc taccatgcag ggttcggccc
     3961 tcacacctct caccgtgata gttggggtgg accccatcag cgaagaagag aaagacatga
     4021 gcctggaaga actggccgcc aagtccctgg aggaaggtaa tgccgaattc atcgccatgg
     4081 gccttgaaga cgcgatcgac gtggtggaat ccggagaagt gaagttcaac agctacaccg
     4141 cctatcacca tctcgtgaag atcgcggtga tgggtatgaa catcaccacc gattcctaca
     4201 tctttaccaa agacaaccaa tctatccagc tcatgattat gggtgatgat acagacatcc
     4261 aggcccaggc tcctcaaatc gcttcgatca agaagagttt caagctcaaa taggtactcc
     4321 gaatgagatc tccggtactg actatcctga gtttactgct gctctgcggc tccgtctttg
     4381 ccatcgattt gagcagtccg gccgcctact ttgacacctt tctgaagacc cgggccgatc
     4441 tgagcggtaa agaaaccgtc tttcactgga ctggcaaggt ctatggcatg gttccgggag
     4501 aaaggcgtac cgagttgttt gccttcgagg gtttcagcgt agccagattg gagaaagccg
     4561 aaacggggta tctgctgttg accagggaag ccgcgttctt caaagatccg cgcaccggag
     4621 agatcctcgg gacctggcgc aatccctacc tcaaaaatgc cgaaacccaa gtcgtccata
     4681 tctggaacga ccccgtcaac caggactttg gtttcagcgc ggaagagatg cccttgatca
     4741 gcaagtttct gcccagcgag gatttgggca gcgagttggt cttttacatg gacctctttc
     4801 ccctctatga gtctcctctg ccccgcaaag aatacagcca gttttcccaa agcgacatct
     4861 atcaagcggc ggagtttttc cagttttatg tgccgaaaag cgcgatcgcg gacagcagcc
     4921 tcaccagtct gccctgcaat atcagctgga cccgcatcag cccctggatg ccatttatgc
     4981 ggatggggga tcgcaaaggc aatctggtgt ttgcctgccg cggcagcaag ctggaaggag
     5041 gttttgaagc ccttccggcc cagatcagga actacgtgat ggaaaaagcc ccccgcttcg
     5101 ctaccgctcc ggatgagtat tccgagccca acgaaaccag ctggacctat ttcaagaaac
     5161 tgatggacgc gggggtgatc aagccgtgat ccgcgaaccc aaaaatagcg caaaggtccg
     5221 gagagctgtg ctttccggat cgtgcttata agctttgagg tgatgatgaa gaagtgtgtt
     5281 gtgaccgcga tcctgctgct gggctttagt tttgccctgg cgcagttctc cagcccctgg
     5341 ctgaatatcc gtcacagttc caggaatatc gatggaaatc tgcagtttcg ctgggaaagt
     5401 gcagacgcga tgctgggacc agctcagttg tgggctcgcc tggggacagg tgactggcaa
     5461 acgcaagaca gcagcctgct ggaaggtgtg agctacgagg ccactccagc ttacacctgg
     5521 ggtcagagac tgcgggtccg gctgaggaat gagatggaaa tggaaggagt gcccgctgct
     5581 atgatgcagg ccgcctggat ggacgacgac actttccctc aggcgcaggg caagctggcg
     5641 ttgatcggtc ccgatcccgt gggggattca ttgatgatcc aggctcccat gctggacctc
     5701 ggcaacacct ggatgggaac cacagatcag aagctctaca gcaccatctc caaccagagc
     5761 ggcagctttc ccaccatgaa cagcctgact tcctacaatg cctggatcac cactatcgcc
     5821 aatccggaag ccatcgcgga cagcgtggtc tatgccatgg tatatacctt caacatcgtg
     5881 ggagtcatct cttccgggct gtataagctg ggaatcgatg agaccatgac ccccaccttt
     5941 gccagaatcg gcagcatcca aaccagcgtg agcggtggca aactgaatat ggcctgcaat
     6001 ctctcagacc tcacgtccga tcccagcttc gggacctggc ccaacctgag caatgcgctg
     6061 ctcttctcca gctttagcat gcggctcaac atagacctca gcaccatgga gccggaaatg
     6121 ctgatcggag actacagcat gccggccata gtatttttcg aagatatcca gtatctgcac
     6181 tcgcaaaata ctctgcctga tgtgacggga ctggacgtga acggactgat cgtgagctgc
     6241 cagtatcagg acgccgaggg agattttccg ctgctctgcc agttcgtagt cgaagacggt
     6301 cccaccctgc agatgcagcc tggttcctat gactttttga ccggagtcac ctactcggcc
     6361 atgctcagca gccctattca gaatggagtg ctgcaggtga gcgacaatct gctttatgta
     6421 caggaataca aatggagcgg agtggcggtc gaagatccct ctctggtgcc agtggccatg
     6481 agctgtgtga tccccaatcc cgtgaaagcg ggccagacgg tcgcgattag atttgatgga
     6541 ctgaaaacag gcaagctgcg ggtggaaatt ttcaacctgc ggggccagaa gctcagcagc
     6601 ctggatcacg atccgcaggg cagccatgcc gcatgtatca actggaacgg cctggccgga
     6661 acccagacac cagccccggg agtctatctg ctcagggcca cacagggaag ccaggtgctt
     6721 acccgcaaat tcagcgttat ttactgaggg agtagcttta gcatccatgt ttattgatca
     6781 cgcgaaactt aaggtgaggg caggcaatgg cggagacgga gcagtcagct tccggcgcga
     6841 gaaatttgtc cccaagggag gtcctgacgg aggagatgga ggtcgtggag gtgatatcat
     6901 cgccctgggc gatgaaaacg tcaataccct cctcgattac cgctttagca agctatacgc
     6961 ggccgaaaac ggcaaacacg gctccggggc ccgcaagacc ggctcttccg gagagcatgt
     7021 gtatctcaga atgcctctgg gcactgagat cttccgggcg gatgaaagtg gccgcaaagt
     7081 caagctggga gagatcaccc agcatggcga agagctggta attgccaaag gtggtcacgg
     7141 gggcaaaggc aacagcaatt ttgccactcc caccaaccag gccccccggc atgccactcc
     7201 gggacgaatc acccatgaaa tggagctgga gctggtgctc aagctgatgg cggacgtggg
     7261 attggtgggc ttccccaatg cgggaaaatc cactttgctg agcgtgttat ctgccgccag
     7321 acccaagata gcagattatg agtttaccac tttggagccc atgctgggag tggtgagagt
     7381 gggcgattac cgttcctttg tgatggcgga tatccctggc atcattgaag gcgctcatat
     7441 gggcaaaggc ctggggcatc agtttctcca gcatattcaa cgcaccagta tgcttctctt
     7501 cctgatcgag gtcacctctt cggaccccgt tgccgcttat cagactctgc gcgccgagct
     7561 ctatctctat gacaagttta tggacaaaaa accctttctg gtggtggtca gcaagctgga
     7621 cctgatcccc gaagaagaac gggacgaaat cctgggagtg attgactctc agttcaggga
     7681 caagtttggc gtgggagtga tggccatctc ctcgatcagc caatccaatc tcgaaaccct
     7741 caaatacagg gtcttcgagc tgctttcaaa ggcatgaccg atcccatcaa gctcagggcc
     7801 tggctgtgcc tcaagacagc acccggtctc agaggaaaag acatcttccg gctgctggag
     7861 cagtggcctc agccccagga atttatcggc aatcccgagc accccgtcta ccagtccaaa
     7921 gactgggacg aacagacgct cagacacatg gccgaaggca agctaccgcc caattatccc
     7981 caggtcagca agctggtgga gctctatgag atcgggctgc tctgctattc agacccggat
     8041 tatcccctgg gtctcaagga gatctacagc cctcccgtca tcctttacta ccggggagac
     8101 ctgatcaaag ctctcaaaca gatctgcctt gctgtggtag gcaccagaaa gcctaccgct
     8161 tacggacgtg agatgtgcgc caaaaccatc gccctggcct gccggaatga tgtaaccatc
     8221 atcagcggcc tggctatggg aatcgatacc gtggcccacc tcaccgcgct cgaacagcac
     8281 agcggcacta tcgctgtcct ggctgccggt gtggatagca tctaccctcc ccaaaaccgt
     8341 gaactggcca aacaaatagt tgctcatgga gctctcgtga gtgagtacga acccggctcc
     8401 aggctggaaa aatggaactt cccagcccgt aatcgcatcg tctcggcttt ggcccaggca
     8461 gtttacgtgg tggaaggtcc gctcagcagc ggagctttgt tgacggccaa gtttgccatt
     8521 gagcaggata gagaactgat ggccctgccg ggtgagatca gccatcccaa cgctcagggc
     8581 cccaactacc tgatcaaaag tggcgcgcgc ctggtctcct gtccggagga tgtgctggag
     8641 gcccttggta tcgaaacaca gccagaccaa cagctgcaga tcatgccgga gctttcggaa
     8701 tccgaacagg gagtgtggga tatctttcaa agtgaacaac gcgagctcag cttcgatgac
     8761 ctgctcctga agacaggtct cagcttcggt aagctctcca tcatcctctt gaaccttgaa
     8821 ctgaaaggct gcatcggcaa agcctctggt aacagctttg ttacgcgttg acagcctcaa
     8881 tattatctcc tgggagttca gcatgaaccg tcgtcttatc atcttgatcg cgatcctggc
     8941 actcagtgtg ggcctgggcg cgaacttgcc ggccgcttac accgctaaag gcaatcccgc
     9001 tctctacgat tcacttaagg tcctggccca ccaaaacctc agcttgctgg acaaacccgc
     9061 ccaacaagcc tacacagacc tgctgcgtcg ctatgacgac atcctgatga actatctgat
     9121 agcctatgag acggatacca atctcgcgat ggcagacccc gcctcgctgg aaagcaacta
     9181 tgcccacatc ctgccactgc tgacgcagtc gaagcacagc ttcagccctg agtttttcct
     9241 ctcctacgtg gcccgtcaga ccgtttccga cgaacgcatc caggcctatc gtaaagcctt
     9301 tctggatgac ggcctgcggg tgctcatgac caaagtcagc gatgaagtgg agctctatcg
     9361 ggaagtgtgt aaatggtgcc tggagcggct tacattccag cccaccagcg gacgcgatca
     9421 aagccccctg gatatcaccc gcaaatccta tctgggacgc tgtgaagaga tgcaaatcct
     9481 gtttgtggca gcggcccgga cggtgggact gccagccaga cctgccagca ctccctggtg
     9541 ggcccattcc gataacaatc atgcctgggc ggaagtctgg ctggaagggg cctggcatta
     9601 caccggagat atggactccg catattatcc cgatcaaacc tggtttagcg gcatgatcga
     9661 caagaccgtc ctcatccttg ccgatggttc tttggcttcc ccaaacgatg aggtcctgtc
     9721 ctccggcagg tatgacaagc tgatcaactc cacttcaaac tacgccaaag agcgtacgcg
     9781 gatgctggaa atcagcgtga cggacgataa gggcaaagcc gtgccggaag ctctgttggg
     9841 catcctggtt tataattggg gggccttgag gcctctcact ttcctcaatg ccgacgcgca
     9901 gggccgtttc aagctcagtg tgggccgggg cgctttctat gtatccgcgt tcaaagacgg
     9961 ccttagcgct ctgaaactgg tgccctccac ggaagataag ctcatggagc tggccctggt
    10021 actcagcgcc aaaaccttgc cagcacagaa tgaactgctg gcttatcctt ccaatccctt
    10081 cgagtggaag caggcccccc agagctggaa agatgacgtt tctgctgtca aagaacggat
    10141 tgccaggcgc gaacaggtct ttgccagtcg ggctatccca gctatttggg aaccctatga
    10201 ctcgctgttt tggaacgtgg cttcagcctg cagaggcaac gatctggaat ttcagttgtt
    10261 tgcttccagg caccggcctg tggatgctga gtttctgagt ttcctgctgg cagacgatcc
    10321 caagctgctc tggcagatgg acgccactca gttcgaagcc ctgtatcgct ttttccgtct
    10381 gcatgcatct gaccggctga gcagcgaagc gctgcactcg ttgatcagcc ccagcgttta
    10441 ttatgaagag ctaccccgtc cctggctcac caagcgggac ggctatcagc tttatccgga
    10501 agactttatc cagccaggta agactcctct gaagaaactg gggcagatta gcaaatggct
    10561 gaataaacgt tacaagacaa atccggacaa ggctttgagc ggtttgctgc ccctgcacat
    10621 tgccaccgga cgcaagtacc tgactgcctt ccagaagcgg atcctggccg tcaacctggc
    10681 ccgcgccaac ggtgtgcctg cagattttac ccgcatcccc aacgtgatct ctgtgatgct
    10741 ggagggtgag tggcaatact atgacatcca gaaaggcaag ctctggagcg gaggtcagga
    10801 gggcgaaagc aaagattttg acctctatgt gaatatcatg gatgaaaacg gcttccccct
    10861 cgagatcgat gccagtcagt tggtcctgac ccgctgggaa gacggcaatt tctatagcct
    10921 gaaccatcgc tttctgcctt ccggaaaggg acgctatact caacaggtcc cgcggggatc
    10981 ttactatctg caaatgggtt atcgcgtctc agacagcctc acgggattcc tgatggaacc
    11041 ggtgatggcg gaaaagctgg acagcctggc cctgatactt caacccaaat catatccgcg
    11101 ctcctggaac gctctcgatc cagccatcgc ggcctggctg gagcctgccg acctgcaaaa
    11161 ccacgccatc atcctgatcg gcaacattga tcatgaaaac agcatccgaa tagccgaaaa
    11221 gctcaaagaa accgataaag cctttttatg gctgggttac aacgaagcga ccgatgcccc
    11281 ttccagctac cgtgtttcca aaacctggca ggaagcggtg cgggcagatc aacgtaatgc
    11341 cgtgagaacg atcacaatta ttaagaccga cagcggctgg cagatgtatg aagggctttg
    11401 ggataaaatg ccctgagagt tgtaaggttt taaggtttta ggtcttaggt tttaggggtt
    11461 aggtgtcata gatccacact gcttactggg tcattgaccc cgaccaagcc atgatctacc
    11521 ctgacccacc tcccataaat ggcttttggg ccggggatgg taaaaggcaa acctgtgcca
    11581 gctaatcagg atggagaaga aaaggctgct ctccctggcc gaaggctctc atcagaccgc
    11641 aggtctctca ctagcgcaca gctctcacag cgaagctctc acttccactc ccccaaagat
    11701 gctgatacct ggctggggca catagggata aacctcataa cgctggttga ggacattgtg
    11761 agctctcaga tgagggtaaa gctgccagcc ctgccattca aagctgcctt ccaagcctat
    11821 atcaagcagt tcataagcgt ccaccggagc gctgagatta tccggagtgg tgaattgctt
    11881 tccagtgtgg gagtaagtgg cccaaatgct taaatggttt agctttatcc tggctgtcgc
    11941 gctcagttgt ctccgggggg tatacatcag ctggggcgcc gcagcgaatg ccaggctgct
    12001 gacatcccgg gcgtccgtga aaagagcttc agcgctgatg ttcagccact taaggccatc
    12061 ccagccagct tccacctcca gattgcgcag ctcggctttg ccaatattga acggcttcca
    12121 ggcgttgcca aacatctgca cctgacgcca ttggatcaga tcagtcacct ggttgagatg
    12181 caccgcccca cgaagattga agccataagc tttcccactg gcccagaact gacctccccg
    12241 ggagcgttcg ctgttcagat caggattgcc gatcgcctgc gaatctcctt tccaatagag
    12301 gtcgtaaggc gaaggcagag caaaggaagt accccaggtg gcccccagac tggtttccgc
    12361 ccagctcttg cagcgcagct ctgtttcagc ccgccagctt gcgacgttct cggttgcagt
    12421 gaggtcatag cgtcccacga tatatgcgct gccgccaagc agatctccgt ctttcaccat
    12481 ccccgcccgg acagtgaaac cagcctgctc tgcttgataa cgcaatgggc tgccagcatt
    12541 taaaagattc tcattcagat agctggtgct tgccgcttca gctcccaggc cagctctgat
    12601 caatccttcg ctcttctcca ggccaagttt tattccggca ttccacaagc cctgtctgta
    12661 acggctgata aacaccggca gaggtgctga agtgttgtca tacagggtgc ggtcctgatt
    12721 ataccaaagc tgggcccgcg agttcaggcc cgccagagga gcttccagac tgatgcggtt
    12781 gcgattggca taacctttta gccaggcgtt gcgatagatt tcgctaaagt tcacggtgcc
    12841 gggcaattgc ctatgaaact cttcaagatc gctggaaagg atcagcaagg attgtccaag
    12901 cctggtactg atgctgccgg agagggagtt ttgacgtttg gcgttgttgc tcctggtctg
    12961 atctccctgc aggccccacc actcccgtgg ttcatactta aaatcattgt cggcattgaa
    13021 tttactcatc gccagccggt aagccaaagc acccagattg cctttgaaag agagcctggc
    13081 ttcggcatat ccaaaggagc ctacctcaat tccagcccgg tattgaggag cctgggccag
    13141 tctgccactc ctggaagtga tcatcaccac tcctccgatc gcggcccctc caccatagac
    13201 cgaggcgttg ttcttgataa tctcgatctt ttcgatgttt tgagggtcca gcaacgagag
    13261 gtcatagctt tcaccctccg cgttcaaagg tatgccgtcc agcaggataa gactgtgccg
    13321 gggcagattt cccagcagcc ggatactctg gacctcaccc cggagcggag tatcggaact
    13381 actggtcccg gaagcctcac ccagcatttg ggtagcgctg aaataatgcc tgtcgggatc
    13441 gatcggcacg ctcagcagat cggccgctga gtttacaaag gagctgtagc tctcgatgac
    13501 cctcacggtg ggaagttcca cgggccgcgc tctgaggctt atcttcagcg taccgctctt
    13561 gatccccaca gcataaggct gaaagcccag tctggaaacc ctcagcgaat ccccttcccc
    13621 ctgaaggctg aaatatcctg ttgcatcgct cagagtccag acctggccaa gctccaccag
    13681 ggcctgctcg atgggcctgg aattctcatc ctggatatag ccgtttacat atacggcaga
    13741 aagactgccg cataaaaaca gcagcaatac tgacaaagac ttagcgcgca tcgggaccgg
    13801 gatagaccag atttggaatc ccactggaag ggttgatcag ggtgctgagc cgcatgccaa
    13861 acagcgcttc cagcttcagc gggtccatca tctcagccgg cttgcctgtt cccaaccccg
    13921 ttccatcttt caaaaagatc agcttgtcgg catagttggc cgccagattg aggttgtgag
    13981 atatcagcag gatgcccttg ccttctttgc ggttgatgcc gcgcagcagt tccatgcact
    14041 cgatctggtg gttgatgtcc agctgagata gactctcgtc caaaaagatg tatttagtat
    14101 cctgggccag agctctggca atcagcaccc gctgtttttc acctccgctc agctccgaat
    14161 aaaagcgctc tgccatggct tccaaaccca ggctttgcaa cactttgccc acagccgcgc
    14221 ggtcctggga cgtccagctt tgcatcaggc ccagataggg ataccggccc atcagcacga
    14281 tatcaaaaac gctgtgatca aactggaaaa gactctcctg aggcacaaag gcgatcagcc
    14341 tggccagttc agaacgctta tagttcctca gctcccggcc aaacagctca atcctgccag
    14401 cctccagacg cagaaatccc aacagggcat agagcagggt ggatttcccg gctccattgg
    14461 gacccgccaa aacggcaaac tcatcttccc tgaggctaat atccagtccc ctgagaatca
    14521 tagtttcacc gtaaccgcaa cgcagtgact gagcttgtat catgtatata ggtccttgtt
    14581 catgaggttc agtttcttca ggctgccaaa tctgtcaatg aaggtttgag taatagannn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa gggaaagggg atttgaatgg
    14761 agaattgaga attgaaaatg gagaataaac gcgtcggttg tgataccagc cttacattgt
    14821 taaagaacct ctgctttcct ctgtgtacaa aatgtttagc attgcaggca caggtagaaa
    14881 cgacgatcca gggtacaaat agaagccatt cagacatgat cccttaaatc cctttttccc
    14941 tttttctgcg ttctataagt tctccattct caatccgctg taaaacaaat ccgtgtaaat
    15001 cagtttaatc agtgtcatct gcgttctatt cattctaacc gccaagattt cacttgccaa
    15061 atatagccta tcaaaatact atgtattaag aagactatta agaggtacac atgggagtca
    15121 acactcgcac cgaatatgcc ctgagggccc tgctggagat gagcggcaac gcggaagagc
    15181 ctatctccgc ccaacgaatc tgcctgcggc agcaactgcc caaaaagtat gtggagcatc
    15241 tcctctccgc tttgaaggtg gccggcctgg tatccagcag cgctggttcc agaggtggct
    15301 acacccttac ccgcaaggct gatcaaatca gcctcctgga cgtaatggaa gcggtggacg
    15361 atcactctct ggagctggaa tgcggagaaa accgcggaga acactgcctg ggcgacgatt
    15421 gccagctcag cccctttttc catcaaatat cccaggaaca gagatcgcta ttcgcctctt
    15481 actccctggc cacgatcgcc agcttttttg agcccagggg agctcgcaaa tgagccagcg
    15541 aatctatatg gacaattgcg tgaccagtcc tttggccccc gaggtctttg aggcgatgaa
    15601 accctatttc agcgagcagt tttggtttcc cggcactttc gtgagcaccg gagaggccac
    15661 caacagcgcc atcgaaggct tccgcaagac tgtggcggat gccttgaatg cccaatcctc
    15721 cgagatcacc ttcacctccg gaggcacttt ggccaacaac atcgcgctca aaggtctgac
    15781 cttggccaat tcagacaaag gccgccatat catcgtctcc gtggtggatt atcctgacct
    15841 gctcaccaac gccgcctact ttgaaagcat gggattcgag ctcagctatc tggaaccgga
    15901 tgcggaaggt atggtctccg cggacaagct caaagccgct ctgagaccag acaccatcct
    15961 cttcatgagc accatggtca atcacgtggt aggcaccatc cagcccattg ctgaatactc
    16021 ccaggtgctg gccgcggccg atcataaaat ctggttccat gtggatgccg gacaggcctt
    16081 tggcaaattg cctctggacg tcaaagccct caatgtggat accctcagcg tcagcgctca
    16141 caagcttcac ggtcctcagg ggatcggagc cctctatgtg aagaaaggaa ccaagctcag
    16201 cccctatatc cacggcgtga agcgctttga cagcctccaa ccgggaggcc tcagcctggc
    16261 agccatcgcg ggatttgcca gagcggtgga gctggtgttt caggacctcg acggcaatat
    16321 cagaaagctg agagagctct cagactatct gctggagcgc ctcaaaggcc aaatagacca
    16381 catcgagctc aacggtcccc gcggagccgg cagggcctgt cacaacatca atgtttccat
    16441 agactatatc gagggcgagg ccatcaccat gatgctggat atgcacggca tcacagtagc
    16501 taccggatcg gcctgcgctt cacaaggcct gaagcccaac tacgtcctga tggccatggg
    16561 caaaaaccat gtgcagtcgc atggctccat gaagtttacg ttgtcacgct acaacaccag
    16621 ggagcagatc gacttcacgg tggcccggct caaagagatc agtgccaccc tacgtgagcg
    16681 cagccccctc tacaccgcta tcaaagacca ggagagataa atgcagtact cagaaaaggt
    16741 acttcaacac tttatgcacc ccaaaaacgt gggcaagatg gaaaaccccg attccaccgc
    16801 cacggaagga tctcccgcct gcggagacca ggtgaccgtc tatctcaaag tggatgacgc
    16861 cagccatacc atcgaggaca tcagcttcct ctcctatggc tgcgcttcca atatcgccac
    16921 cgcttccatc atcacagaaa tggccaaggg caagaccctc gaagaagcca aacagatcac
    16981 ctggagagac gccatggaag ctctggacgg tttgccaccc gtgaaagctc attgcagcgt
    17041 cctggcagcc gacacactcc agaccgccat ctcaaactat gagatcgccc acggcctcaa
    17101 ggaagtgccc aactttagca aagacaccat catcgaagag cttaaaaaga tcatctatcc
    17161 caaagtgggt gaagacatca tcaccctcaa aatggtcaaa tatgtgggct gggacaatgg
    17221 cgaagtgctg atcgacctca acctcctgag ctttgatcaa taccgcgaca acgtggccga
    17281 agaaatccac gaacacctgg agaagtatcc cgaagtcaag aatatcgtga tcaacttccc
    17341 aagctgagaa ctgggttgct ttcccgcaga ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17461 nnnnnnnnnn nncacgccgt tcctggtctg gccgacatta agcttgacag gattctgcca
    17521 tctggtttca tctacagcat atgaaaacat gaggagatag agttaatggt tagccgcagt
    17581 ttaaccagcc ttttttggga ataccagata tccgagcagg aactgcagga ctgcctggat
    17641 aaaaatgatc ttaacgatcc tataactgtg tcgatctaca atcgcctctt acttagtact
    17701 cccgactggt atagcctgtt agatatgcta agttgcgagc aactcaaggc cgcgttgtct
    17761 ccaacagtga tcaaaaccat ccactccaag gccttgcgag aacgctacag cttcgcggcc
    17821 agcaggctct ttcctgaaaa ataacatgga caggtccttg taccacaatg ctctttatcc
    17881 actgcaggac acagtcctat ccctggttgt ggaactcgat ctgccattct atctcacagg
    17941 ggggacagcc ctgtccaggc actacctcaa tcatcgctac tcagatgatc tggacctgtt
    18001 cgtcaactcc caccccgatt tcagaggtct ggtcgatgct atcgaagccg gcttcaaaga
    18061 taagcaactc acttatcaag ccattacacg cagtgccgat tttgtgaggc tccaagtttc
    18121 ggggacggat gatacagtcc ttaaagttga tctggttaat gacttagtct gccacattgg
    18181 tgagtttgag cgctctcctc aacttgggtt agtggataac gtgttcaaca tcctctccaa
    18241 caagatttca gcgctcccca ggttggcaat caaggacttt gccgatatcg tctttatcgc
    18301 ccgcaaatac acgttcaact ggaaagtcct gatcggtgag gccttccaaa aggatgaatg
    18361 ggtaaatccc ctcgatcttg ccagatattt tgccgaagtg cccccaaaca gatttgaact
    18421 gatccaatgg gtcgaacctg tgaacgtcag cgaaatcata ctaagctgcc agaaaatcgc
    18481 cgatgagatc atcctgggag cagacaactc actggcgcag aagtaacagc cgtcctgagt
    18541 ttcaagacaa tcactggatc aagattccga cccgtcccgc ccctattacc cgccgtagag
    18601 ctgccgtgcg ctctcccggg tttttcgccg gaggccgcac ggcatctcca cggcaacaaa
    18661 caggcaagct tgcggaggag tgcaatctga atatgttatc agaatagcaa acgaagaagc
    18721 cactggaggc cctgatgtat cagcctgaca tggtccccgc ggaccctcag taccgcagac
    18781 aggtgctgat attgctgttg ataatagccc tgggactggg atttctgctc tatagcctgc
    18841 agccgtatct gcaggcaaac atcgataccg gcacgcctga acagaccctg caactgctga
    18901 agtggatgct gatcgccctg gggattgtgc cgctgggatt tgcgatctac atgctggtga
    18961 tcgcgatcaa ggtttacagg cagggtattt tccctccgga agggaccaaa gtgctgaggg
    19021 acacgcctat cctctttgat caggatgcca ggcggcgggc caatatcctg gtggtcctcg
    19081 ccgcgttgct gatgttcatg tccgatctgc tgatcctgat ggccttctat ctacccaaac
    19141 tgctgacaag gttgtagaaa tagaacgcag ataacgcaga tgaaaggatt tacaagatat
    19201 tgaatggctc tgttcaggag tcttgatgaa agcaaaccgc ctcatttcaa accagaaatg
    19261 aggcggtgtt ttataatgat cagatcagtt gactgccgtc accttataga atctctgagt
    19321 ggaggaagc
//
LOCUS       DAOSFN010000029        19304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2297_length_19304_cov_9.083589, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000029 DAOSFN010000000
VERSION     DAOSFN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 19304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19304
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2297_length_19304_cov_9.083589"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLO35_03310"
     CDS             <1..298
                     /locus_tag="PLO35_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78724.1"
                     /translation="VPTELKMPVFAQALKLVLRSYTTVSSIKTTVPLGLMRSQFLRYH
                     LTNSDASIHSIDYHADTFQKSHESRINVWGNRGSPTQFEREHHKTASILHQATS"
     gene            516..959
                     /locus_tag="PLO35_03315"
     CDS             516..959
                     /locus_tag="PLO35_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78725.1"
                     /translation="MKRESKHIPADFTGSVDELIFYRHKANGKIYARKRFTFKNHPGQ
                     PPFASAQKAIYALQPSQAYRQNLSDYLIGYNKLKINDGREVIAWTNLYNKLMFAMQKQ
                     LGIALTGITREMIVQQNLPCQTVKAAVEAGLLPKVPDYERFNQPI"
     gene            1046..2284
                     /locus_tag="PLO35_03320"
     CDS             1046..2284
                     /locus_tag="PLO35_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78726.1"
                     /translation="MRKLIFFSLMAVLLVAGCKTHTTQPTQTGELPVRMDLSPAAQFN
                     ITVTRVEVTIVKGSFSQSMDLAINGSLAEGTFEDLEPGVYAIDVRVYEGLTLIATGHG
                     TGVVSPSQTTTVHITLQFVPGGLEIVVGWGLPYEASRRVLLVGNSHTYVNEGVDTHLQ
                     ALMAAAQPEWNVVVEDRTAGGYTLENHYHDQNTLDAIATGDWDLVVLQEQSSRPMLYP
                     ELFYAYADSLHTLIQQSGALSGFYMTWAWRNNPEMYVPIRDAYRYIAAYLDALVVPAG
                     VAWYNADALHGMPNLYDTDNYHPSIYGTYLVACLMMARIWNLNPVGNSYYPAQIDAAS
                     ALMIQQLAWDTHQHEYRDLEGGLLLEWDAMTEASSYNILPSAHPHATDRGTPVYNTTD
                     LWFTAPPNDERVFYRVTSVN"
     gene            2314..3717
                     /locus_tag="PLO35_03325"
     CDS             2314..3717
                     /locus_tag="PLO35_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH78727.1"
                     /translation="MKKNLALMLILSWLTIPLLMAQIEGLSIPGRSKLQYSQTDVRSY
                     YPLEPAGPAQTENNAQTTFWTGFSTCGHGFTLVSCMIDSIGQDQIKTTIARQSYTFNF
                     PSSTPVFEDGHPISFRDLKKGDRITVSAYLLDPKDLRWYSFRSRWFDSRYFADTEIDK
                     GWLRGRLELDPAKQVPGKIEWLCHCERRARLAGKVLSSKGPYEGENPKQGFRDRQTIL
                     KAIGENMVRVPGGSYQLWKAEALVVQPYYISKYEVSADEYKLVMNQLPYSSNSTVGNY
                     PVRGISWYDSIEFCNLLSIMMGLDPCYSYEGHGPDPGTWPQGWRGNEDDHVKIILNPA
                     ANGYRLPGNSEWLIAFHGATHTWTHKTFSQYALDRTAWHAGNSSGTIHTIGSKAPNEL
                     GLYDMLGNVFEYVFDIMNVEPDPRYPTLVPGVYRTAKGEDLNSKIDHSYLNTEMTINP
                     TSTYHDAGFRLARNAEQ"
     gene            3873..4382
                     /locus_tag="PLO35_03330"
     CDS             3873..4382
                     /locus_tag="PLO35_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78728.1"
                     /translation="MKDKFVRYLIPVLLFGVFVCSAWAQEQALPELSLTEVREIELPQ
                     GFTEGLKDGSLTSPNSSLWFVAGCLFPLPGLIVSAAYSPKFNPQELEKITQLKGQNYA
                     TGYAKAYETKARNKNLVSAATGFGISISISLIISSLSNLASATTMANPEARTDIPIQL
                     IKELRYDTH"
     gene            4369..4881
                     /locus_tag="PLO35_03335"
     CDS             4369..4881
                     /locus_tag="PLO35_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78729.1"
                     /translation="MTRISLCLVIIVGILGLVGCSQVPVATKMVPSAFSMYKRDLPRS
                     ISIGKVYGCDPSLKSNKYKITDPVFKQALGDALNNSKLFQKAVYDDLGSNYVLEAELA
                     YNEMKAGFGGHPMELFVRYSLYDQINGVLVWSKDIYSYSNKGSAKAKTEQAGRANILR
                     LIQELSKLEL"
     gene            4892..5272
                     /locus_tag="PLO35_03340"
     CDS             4892..5272
                     /locus_tag="PLO35_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78730.1"
                     /translation="MGFKAGNQIVAGRIWLVVCLLMVIISITACRPSNKRFEGAIWDA
                     YRYGKVAKQSSDRVATEIPESLKILKVSKMEADDGGDIYLAGVELDFTWGKTGGLFSL
                     RKEKQFWIAEYVDGSEGMTVDKDR"
     gene            complement(5330..5863)
                     /locus_tag="PLO35_03345"
     CDS             complement(5330..5863)
                     /locus_tag="PLO35_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH78731.1"
                     /translation="MSYIIRTATSQDASGMAAVHIQSWLETYPGIMPQQKLDSLNLEA
                     STRNWSNAIATCEVVMVAELEGRIIGFVSGGKNRSQEDCETGLANFCDAELAAMYMFA
                     EFHGRGIGRAMFEAFTSRLRSLGYKQMAIWVAAKNSSTGFYRKMGGLLIDSRDLTVCG
                     EQVPLLAYRCELGWARI"
     gene            complement(6087..15749)
                     /locus_tag="PLO35_03350"
     CDS             complement(6087..15749)
                     /locus_tag="PLO35_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78732.1"
                     /translation="MRRSIVLLFVILCASQGVANYVENQAYEIVQPDGSKFMAYITGD
                     EYYHRVHDKAGFTILLQPGTGYAVYAVPGLDGIMPSSHIAGRVDPSLLGIRPGLMPQE
                     APVRARFEEEQRLRQIYRANPIGTINSIVAFVRFEEQDEFPTSKTYSQYSDMFNLMDT
                     ASLKDYYNEVSSGQLTINSFLYPGPNGSGQVVSILVPWERGYFSPYIEDVNDIGYPEG
                     EGGPRKRELFEYVVDQLDTMVPNDLNIDMDNDGRVDGVTLIVCGPTDAWGDLLWPTSN
                     SSGTPFHAINGKDVHSVILNFQQSLGISVICHEMGHQIGAPDFYHYHEWEPWHSIHPV
                     STWCLMASDRAQHWLTHNKWKYGHWFDAIPTLTATANPTTYTISSVNESPYSCYKIIS
                     AVPDQYYMVEYRKKAGLYENNLDGSGLIVYRVTTGINGNAGGPPDEIYVYRPNGTLTE
                     NGQPGNAFFSQESGRTEIFNGSNPSPWLYADTLSTIAGDIVITNVGSSAGNSISFTIN
                     TIAPNTWTGEINESWTNAWNWTQGVPGSSDDVIIPSGCAQYPNLNGGGTGSCKKLTLA
                     NGGIIKVGPGTLNVHGTAHLSGQIQLCASAGTVDFKGHTNSYSGSSIIDSAAGMLKFN
                     GSWKVDSGCTINLDQSTVSFYGAEDAELQCDALVNLKHAYVSKIYNKAVYYQSSLARL
                     QVNGTFSLAAGGGFVQNADSDLQIAGSMLGSGYFRQEAGTLKAIGSTAQTISMSGSSY
                     INNLWINGSAQLNLQSALNIRGDVSVSGTINAGGYAMTVWGSWTGTGTLNGTDNRVIF
                     AGSGISEVQQLDAQILELAKTGSGTLLLGTGKVVSCQQYDWTSGRIIVSGGSFSASDL
                     YDPHIAGTFTLSAGAINLAQDGDNSLDINADITITGGTFTIVGGSRCNLAASRSVNFT
                     MGGGVLELGIRGMVYESGCYALTSNLYGGTIRTAGSLLITRGGFVMTGGTVEPFGGLN
                     SSVIVNAPSRIHHLNVAKSPSNTVYINGNTVVGGNATITTGRLEIQDCTLGIAGNLTI
                     TGTLAMNSVADIVNVEGSVIWSSASTCDIVSGTLAIKGNMNVNNSTSFTMGDASILKF
                     YGTSPSALSTGLSTLTLGNILVQKTGSSCTLDPTGMIKLFYANDVVVYGGSTFNPYNN
                     AMIVEGKIDIWEGGCLTVNNNAEISAYNFYCSGLYNQSNGLVYVNNNYIQYATGTNQI
                     TGGTFRIDTPYGGSHYSFAGTTTLSGGNFQITNNGIQFGIGSSFNMSGSGTLKLGWGL
                     RALSESVFQASSGTVQFIGERSSNINLAAGNYLPNIVIDKAGSTGAVYLLTDLTVKKS
                     CTVQNGRLYVNERTLTVEQDIVIQGGDLYAYNSTDHIRFGGSWTNNASPENFIEGSGL
                     VTFIGGGLTTTIGSPETFNHLRIDSSPSARLILAADMTLSINADLEISSGQLRILDGS
                     HLNVLGNMSISGANSVVDLDFNRTVDPTDTHIHGNLLINGGILKSQTTNAAIALDTFL
                     IDGDLAMTGGELDVRRAAMLVHGNFSTTATSLFKTREGTFVNDAPYTGTWQIANCAWT
                     TNGSTIEFPNKGLQFVSGAQWVTDGFSVIRIGRGFYAVSDDAFDPGNGTIEFIGSVQA
                     NINMGANNTLPSVTINKPGSMLVLSTPAIVTGSITITSGNFNSNGHALTIGGDWVNNV
                     GSSGYTSGTSEVVFRDQYAYLPLHSLIGAQTFHKVTVDYPLSTQYLNSSGNSTVQNLL
                     NVVSGGLRVNGTLQINSDVSIAQNAYIEVTGGDFYLAGNLSDSNMTYSGSNPKLGLIN
                     ASGSVILNGSGNQTIAVGYAEIGLNNLFLIKSSGIFQPAKPVNLKGNATITSGTWGYG
                     ASGLTHKIGGNLNIASGGIWNDNTGTVSFVGTGNGVLSNSGTASFKNLHIDKSSSRTT
                     FVSLSLGSNIALNTSGTITIDGGCLDLGAYSLQTKGSVNVNSDGKLAIGPGGILTMYS
                     SSSLSVNSGGVLETLGTSEAQALITSLSGTYNLEVNSGATLSSEWTIFEYPGSNGIRI
                     NSGALVDPTHALHHCTFRFGTSGGPLLAIDNAQTFQIQDARFPSGGSQTFNVSKTLAQ
                     GEVSFNGESGGFAGTGHENDPFGRVNWSSDVPEIQVNPGAFAFGDVTWMSTSSQYMVI
                     ANPGSAILRGDIHMPSGFSVSFVGTRTAEARELIPEAKSEQEFSRNDMGFSYLPGTSG
                     IYMITFAPQEPIPYSGYMVVTHNADQPAFNLLCSGNGVGAKLFLDNTQLQFDLLPGQS
                     GSILTNLTDAGTDSLSYFGYVNYMRDRDVILETGFEDAMPPAGWNETPVSGTAGDWLR
                     YSSTVHPSGNPPSSGSWLGYFNSYTSNVGNSSRLESPVLDLSLKTSVMLSLWMFHDSG
                     YDTRDDRIQVQVSIAGGAWTDVGAPISRYNGQWGWHQHYVDLSSVNHAANVRVGLLGI
                     SAYGNDIHIDDLSITGTTQLPSNWITLNGGASVSGLIFPDDPAVPITVSVDTTGIPQG
                     FYMNQLVFMSNDPVYPFYALPLMVRIGVPAYETSPATLEFGSLQVGMVSMLGFSVEST
                     GSIGLSGTVTTPAGYEIITIPGPRGRSDLSGRPEEGLRNQTSFYLYPGESRDFMVGFG
                     PMEAQSYDGQIIISTNTGTDTYVDVTGEGISQPQVNTISVTDIQAASAICHAELSSTG
                     NLLISARGIVYNTWGDPDLDDGNVLLSEGQGGGFDLSLSGLVHSSTYYVKAFATNTLG
                     TVYGEELSFSTLTPQLSLSTHVLPDFGHVPIGTTSAPASFTISGEHLVDMVMMSSHSG
                     FTISLNQSSGYDSLLLLYPTAETLPETTVYVRFTPPSTGAYTEVITPLSVGTAAGEIA
                     LSGLGVTIPQVHISIPFNITSSSAEIGGLVQSDGLSPVTACGICWSLNPSPTVSDWHS
                     TLMYQPDWYYSLMEGLDSGTSYYARAYATNVAGTAYSSQSVFSTHFLPQISVLPAELP
                     SFGEALVGSVTPAAQLTIQGSILGDELTLSVGGNYGLSLSSRDGGRVFTQTIQIPAVD
                     SLIAPTSVYVRFQPQSGGNLDDLILCQAEGVEDLLIPVYGTGIGLPTLSTTPASNLAS
                     YSATVGGNILADGFSAINACGVCFDTSPGVDLDGPHTSNSALQGEFIDYLLALLPDTS
                     YYIRAYATNAAGTAYGEELSFATPADLIAAPQHLRISIAGPNVLLEWDPVLDAEYYKV
                     YALDFYTTSSETWPLPQICVDTSLSMSITELGARKFFFVIACKE"
     gene            complement(16177..19110)
                     /locus_tag="PLO35_03355"
     CDS             complement(16177..19110)
                     /locus_tag="PLO35_03355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78733.1"
                     /translation="MRKTLLTACLLLLVLLGLNAEEIAIRKADDLELQTFSFKAADGS
                     RLVFWSDLSSGDRDIWCQKISDSAQVLFDEAVPLVTAPGDQDLMSVVASSDNNFIILW
                     AEREIEEVTQLKVQKVTSNGQLLWGSGLPVSSLPIDMPEVALVPNSLGGAFVIYQSWY
                     NQDIMGQNFDSFGNRLWPLAGQLLVDNEVHIHLDAAVSDGSGGTILSVKYWINSVWKS
                     SLLRFSPTGSQIGTGSLVPAGTFSGPVYQILPLGNGQYLLYQETDGSTKIFYMNKIDA
                     SGTLLYPQAVAFAMSGTEYAVVKGLTGTPEGGVAIAWKGSEWEGGDQIFVQKLSSALA
                     PVWPQPVSIAGSDSNISSIQINSGADGKLWTSWQGYYDDPFFIAQVLDANGSPIWEAG
                     GKLISARAREVLSFGGQTLGFYLWSTTESGYKSIKLQALSVNGAFSYPPGGLTLEERL
                     NYNCSIVGAYSLSDRFLSLWIDRRDRDNLYFQIINQNTQALLEPGGRAVLSTSFDVVN
                     VNAAVTTPEGKLAIIYSTEDYSTEPATYATYLQKIDANGNLEYPGNGLSLSVDTTEAA
                     SMSCDGNAILIGWVKSVYGQPQELRGQRIVNGQALWGQYGKLIFSPPANHYLLFKGMA
                     GSYFLWEVYSSGTGYSNCKALRVDANGDPAPGWDSSGVNLITAHDYIDEIYDVTGLIG
                     DDLVAFISLSRPGLYGKRVQRLSSSGARLWGEGGTLVGGTDTWMNIYPAIFEDGIIFL
                     TSNPDEAIVLRRIDSQGLETTPLQGIVVVPETSNCYDATLIKFANGSLLCAYSDNDGA
                     WIQNRDVFIRHISPEGIPGGDGPIALCSERYQQDYVRVAVLGNRALVAWADDRAGIIN
                     SEEAWTGIWGNMVTSAFTAADDPQLAPVSHPTLTGNYPNPFNPSTSISFSLPATGPVS
                     LGVYNLKGQLVKILLSDAELASGPHSVIWDGTDARGQMVSSGLYFCRLSTARHTSTRK
                     MLLAK"
BASE COUNT         4409 a         5260 c         5226 g         4409 t
ORIGIN      
        1 cgttcctacg gaactcaaaa tgccagtttt tgcacaagct ctgaagcttg tgttacgatc
       61 atatacaacg gtctcatcta tcaaaactac cgtcccgctg ggactcatgc gatcgcagtt
      121 tctgcgctat catctcacaa actctgatgc gagtattcat agtattgatt accatgcaga
      181 tacatttcag aaaagtcatg aatcccgaat caatgtctgg ggtaatagag gttcacccac
      241 ccagtttgaa agagagcatc ataaaactgc atcaatactg catcaagcaa cctcataaac
      301 ctttcagctc cttcctcccc gcatcccatt acggactcat tacggactta gtccgtaatg
      361 agtccgtaat aactccgtat tgccaagcgg caggaatgga ttatactatg ggtatgcggt
      421 gggccgcaga gaatctgctg gatggcctta tatggctgtt tatcaagctg atagagccca
      481 aagaagccgc gaacaaacaa ggagaagcaa gatatatgaa acgagagtcc aaacacattc
      541 ccgccgattt caccggcagc gtcgacgagc tgatctttta ccgtcacaag gccaacggca
      601 agatctatgc ccgcaagcgt tttactttca aaaaccatcc cggccagccg ccctttgcca
      661 gtgcccagaa agccatctac gccctgcagc catcccaagc ttacagacaa aacctcagcg
      721 actatctgat cggctacaac aagctgaaaa tcaacgatgg ccgtgaagtg atcgcctgga
      781 ccaatctcta caacaagctg atgtttgcca tgcaaaagca gctgggcatc gcgctcaccg
      841 ggatcacccg tgaaatgatt gtgcagcaga accttccctg ccagaccgtt aaagccgcgg
      901 tggaggctgg actgctgccg aaagtgcctg attatgagcg gtttaaccag ccaatctaag
      961 ctcaaattgc cagattgagc gttgtgaaga gcgggaataa taagcccggg ataaaccgcc
     1021 aaagctcaca aacaaaggag acagcatgcg caagttaatc tttttcagcc tgatggccgt
     1081 cttgctggtg gctggctgca agacccatac cactcaaccc acccagacag gtgaactccc
     1141 tgtccgcatg gacctcagtc cggcggccca gttcaatatc accgtcacca gggtggaagt
     1201 gacgatcgta aaaggcagtt tcagccagag catggacctc gcgatcaacg gcagtctggc
     1261 tgagggcacc tttgaagacc tcgagccggg cgtctatgcc atcgatgtga gggtctatga
     1321 aggcctcact ttgatcgcca ccggccacgg caccggggtg gtctcaccct ctcagaccac
     1381 gacggtccac atcaccctgc aatttgtgcc tggcgggctg gaaatcgttg tgggctgggg
     1441 acttccctat gaggccagcc gcagggtcct cctggtggga aacagccaca cctatgtcaa
     1501 cgaaggggtg gacacgcatc tacaggccct gatggctgcg gcgcagccgg aatggaatgt
     1561 ggtggtggaa gacaggaccg cgggaggtta tacactggaa aaccattatc acgatcaaaa
     1621 caccctcgac gccatcgcca ctggcgactg ggacttagtg gtcctgcagg agcagagcag
     1681 ccggccgatg ctgtatccag agctctttta cgcctatgcc gattcgctgc acaccctgat
     1741 ccagcagtcg ggagctctca gcggattcta tatgacctgg gcctggcgca acaacccgga
     1801 aatgtatgtg ccgataaggg atgcctatcg ctatattgcc gcttatctgg acgccctggt
     1861 ggtcccggcg ggagtggcct ggtacaacgc ggacgccctt catggcatgc caaacctata
     1921 tgacacagat aattatcacc ccagcatcta tggcacctat ctggtggcct gtctgatgat
     1981 ggcccgcatc tggaacctca atccggtggg caatagctat tatcccgctc agatcgacgc
     2041 tgccagcgct ctgatgatcc agcagctggc ctgggatacg caccagcatg agtaccgcga
     2101 cctggagggc ggtctgctgc tggaatggga cgctatgacg gaggccagct cttacaatat
     2161 ccttccatcc gcccatcccc atgccacgga ccggggaacg ccagtctata ataccacaga
     2221 cctctggttc accgctcctc ccaatgacga gcgggtcttt tacagggtta ccagcgtgaa
     2281 ctaacgggat ctgcaatcca gggagctaat cagatgaaaa agaaccttgc tttgatgctc
     2341 atcctgagct ggctgaccat tcctctgctg atggcccaga tcgagggatt gagcattccc
     2401 ggccgctcaa aactacagta ttcccagacc gacgtccgca gctactatcc tctggaaccg
     2461 gctggaccgg cccagacgga aaacaacgcc cagaccactt tctggaccgg cttcagcacc
     2521 tgcgggcatg gctttacgct ggtgagctgc atgatcgaca gcatcggcca ggaccagatc
     2581 aagactacca tagcccggca gtcctatacc ttcaactttc ccagctccac cccggtcttt
     2641 gaggatggcc atccaatcag cttcagggat ctgaagaaag gcgaccggat caccgtctcc
     2701 gcttacctgt tggaccccaa ggatctgaga tggtacagct tccggtcccg ctggtttgat
     2761 tcccggtact ttgccgatac cgagatagac aagggctggc tgcgcggacg gctggaattg
     2821 gatcccgcca aacaggtgcc cggcaagata gagtggctct gccattgtga acgcagggcc
     2881 aggctggctg ggaaggtgct gagttccaaa ggcccctatg agggtgagaa ccccaagcaa
     2941 gggttcaggg acaggcaaac catcctgaaa gcgatcgggg aaaacatggt gagggtgcca
     3001 ggaggcagct accagctctg gaaagccgaa gctctggtgg tgcagccata ttacatctca
     3061 aagtatgagg tgagcgccga cgagtataag ctcgtgatga accaactgcc ttacagcagc
     3121 aattccacgg tgggaaacta tccagtcaga gggatttcct ggtatgacag catcgagttt
     3181 tgcaatctgc tcagcatcat gatgggcctg gacccctgct atagctatga aggccatgga
     3241 cctgatcccg gcacctggcc acaggggtgg aggggcaacg aagacgacca cgtgaagatc
     3301 atcctcaatc ccgcagccaa tggctacaga ctgcccggca actcagaatg gctgatcgcc
     3361 tttcacggag ccacccacac ctggacgcat aagaccttca gccaatatgc tctggacagg
     3421 accgcctggc atgccggcaa ctcttcaggg accatccaca cgatcgggtc taaggcaccc
     3481 aacgaactgg gcttgtatga tatgctgggc aatgtctttg agtatgtctt cgatatcatg
     3541 aacgtggagc ctgaccccag gtatccaact ctggtgccag gagtatacag aacggccaag
     3601 ggcgaagatt taaactccaa aatagaccac agctatttaa acacggaaat gacgataaat
     3661 cccaccagca cctatcacga tgctggattt cgcctcgcca gaaacgctga acaatagggc
     3721 gtttccacgc ttcagggttt gctccggatg cggctgagtg gaaaagattc aatcgcgctt
     3781 ggcgggtttg tgtgctaaga ccgacattta tgttgacagg tagaacattg tctcccaagg
     3841 tggatacgca aaaatgcaga aggagctaac ccatgaaaga caagtttgtt aggtatctta
     3901 tcccagttct attgttcggc gtctttgtat gtagtgcttg ggcccaggaa caggccctgc
     3961 cggagctttc cctcaccgag gttcgtgaga tcgagcttcc ccagggattc acggaagggc
     4021 tcaaggatgg cagtctgact tcacccaatt cttctttatg gtttgtcgcg ggatgtctat
     4081 tcccgctgcc aggtttgatc gtatcggcag cctactctcc caagttcaac cctcaggagc
     4141 ttgaaaagat aacccagcta aagggtcaga actatgctac gggctatgcc aaggcttacg
     4201 agaccaaagc cagaaacaag aacttagtgt cagccgcgac tggattcggg atatcgattt
     4261 cgatctctct gatcataagc tctctatcca atctggcgtc agctacaaca atggcaaacc
     4321 ccgaagccag gacagacatc cccatccagt tgattaagga gttgagatat gacacgcatt
     4381 agtctatgcc tggtgatcat agtcggaatc cttgggttag tcggttgctc tcaggtgccg
     4441 gtggcaacaa aaatggtccc ctctgctttt tccatgtata aacgtgacct acctcgttct
     4501 atcagcattg gcaaggtata tgggtgtgat ccctccctta aaagcaacaa atacaaaatc
     4561 accgatcccg tctttaaaca agctctgggg gacgctctta ataattcaaa gctctttcag
     4621 aaagcggtct atgacgactt gggctcaaac tatgtccttg aggcagaact tgcttacaat
     4681 gagatgaaag caggctttgg tggccatccc atggagctat ttgtccgcta tagcctttat
     4741 gatcagatca atggcgtcct cgtgtggtct aaagacatct attcttatag caataagggc
     4801 agcgcaaaag caaaaaccga gcaggccgga cgtgccaata tcctcaggtt gatccaggag
     4861 ctttccaaac tggagctata agggaataac aatgggattt aaagctggaa accagatagt
     4921 tgccgggagg atttggcttg tcgtctgtct attgatggtc ataatatcaa ttactgcctg
     4981 ccggcccagc aataaaaggt ttgagggggc aatctgggat gcctacaggt atggcaaggt
     5041 tgccaaacag agttccgata gggtggccac ggagataccg gaatctctca agatattgaa
     5101 agtaagtaaa atggaagctg acgacggtgg cgatatatat cttgctggag tagagcttga
     5161 tttcacctgg ggaaagaccg gcggtttgtt ttcacttcga aaagagaagc aattctggat
     5221 cgccgagtat gttgatggat ccgaagggat gacagttgat aaggacaggt aatgcacctg
     5281 aatctgatta tgtaagcgtg ccggatgtat ctttataacc agggttctat caaattctgg
     5341 cccaacctag ctcacagcgg tatgccagca gcggtacctg ttcgccgcac actgtgaggt
     5401 cccggctgtc gatcaggagg ccgcccatct tcctatagaa tccggtgctg gagtttttgg
     5461 ccgccaccca gatggccatc tgcttgtagc ccaatgatct cagacgggat gtgaaggctt
     5521 caaacatcgc cctgccgatc ccccgaccgt ggaactccgc aaacatatac atggccgcca
     5581 gttcggcatc gcagaaattg gccagtcccg tttcacagtc ttcctgagag cggtttttgc
     5641 ctcccgagac gaagccgatg atccttcctt ccagctctgc caccatcacc acttcacaag
     5701 tcgcgatggc gtttgaccag ttgcgggtgc tggcttccag attgagtgaa tccagcttct
     5761 gttgcggcat aatgccagga taggtctcaa gccagctctg gatatggacg gcagccattc
     5821 cggaagcgtc ttgtgaagtg gcggtcctga tgatgtatga cattgatagc tgagcttcct
     5881 ttttctgtgc tgttttctga atcttgagcg acccagaata tgacccgaag ctatatgtca
     5941 agcaatgaat agctgggcag gaccgacgat ggcttcagaa cgtgaaccac ggctgatcat
     6001 accggcgtca agctcagact gggcatccaa gactcaggtc cccgcggaga ggtgattcca
     6061 tcaagtggat cagctaaagc agtcaatcac tccttgcagg cgattacgaa gaagaacttt
     6121 ctggcaccca gttcggttat ggacatgctg agcgaagtgt ccacacagat ttgaggtaag
     6181 ggccaggtct cgcttgaagt agtgtagaaa tcgagggcat agaccttgta atactccgcg
     6241 tccaataccg ggtcccattc cagcaggacg tttggcccgg caatgctgat cctcaggtgc
     6301 tgcggagcgg ctatcaagtc tgccggggta gcgaagctca gctcttctcc ataagcagtt
     6361 ccagcggcgt tggtggcata ggccctgatg taataggagg tatccggaag cagggccagc
     6421 agatagtcta taaactcacc ctgcagagcg ctgttggatg tgtgcgggcc gtccagatca
     6481 actcccgggc tggtgtcaaa acacactcca caagcgttga tggcgctaaa accatctgcc
     6541 aggatgttgc ctcccaccgt ggcggaatag ctggcaagat tggaagccgg agtggtgctg
     6601 agcgtcggca gtcctatgcc agttccatag acgggaatga gcaggtcttc cacgccttcg
     6661 gcctggcaga ggatcagatc gtccagatta ccaccgcttt gaggctggaa acgcacatag
     6721 acgctggtgg gagcaatcag gctatccaca gcggggatct ggatagtttg cgtaaacacc
     6781 cgacctccat cccggctgga taaggacagg ccataattgc caccgacact taaggtcagc
     6841 tcatccccca ggatgcttcc ctggatcgtc aactgagcgg ctggagtcac ggagccgacc
     6901 agcgcttccc caaagctggg cagctctgca ggcaacaccg agatctgggg caggaagtga
     6961 gtgctgaaca cgctctggga gctgtatgcg gtcccggcaa cattggtggc ataggcacgc
     7021 gcgtagtagc tggtgccgga atcaaggcct tccatcaggg agtaatacca atccggctga
     7081 tacatcaggg tgctgtgcca gtccgagacc gtggggctgg gattcaggct ccagcaaatc
     7141 ccgcatgcgg tcacagggga taggccgtcg gattgcacca atcctccgat ttccgccgat
     7201 gagctggtga tattgaaagg aatgctgata tggacctgcg ggatcgtgac tccaagcccg
     7261 gataatgcta tttcgccagc cgctgttccc acgctcagag gagtgatgac ctcggtataa
     7321 gctcctgtgg atggaggagt aaagcgcaca tagacggtgg tttccggcag ggtctcagcc
     7381 gtcggataga gcaggagcag ggagtcatat cccgaggact ggttcagtga tatcgtgaag
     7441 ccgctgtggg agctcatcat caccatgtcc accaggtgct cgcctgagat ggtgaaggat
     7501 gccggagcag aagtggtgcc gatggggaca tggccaaaat ccggcaggac gtgggtggat
     7561 aagctgagct gcggagtcag ggtgctgaag ctgagttctt ccccatagac agtgcccagc
     7621 gtgttggtgg caaaggcttt gacgtaatag gtgctgctgt ggaccagccc gctcagagag
     7681 aggtcaaacc caccgccctg accttcggag agcagcacgt tgccgtcgtc cagatcggga
     7741 tcgccccagg tgttatagac aatcccgcgg gcagagatga gcaggtttcc ggtggaactc
     7801 agttcggcgt gacagatggc actggctgcc tggatatccg taactgatat agtatttacc
     7861 tgcggctggc tgatcccctc cccggttaca tccacatagg tgtcagtacc ggtgttggtt
     7921 gagattatga tctgtccgtc gtaggactgc gcttccatgg ggccaaaacc caccatgaaa
     7981 tcgcgtgact cgccagggta cagatagaag ctggtctggt ttctcaggcc ttcttcaggc
     8041 cgcccggaaa gatcagacct gcctctgggc ccgggaatcg tgatgatctc gtatcccgct
     8101 ggagtcgtaa cggtcccgct gagcccgatg ctgccagtgc tttcaacgct gaatcccagc
     8161 atcgacacca tccctacctg cagtgatcca aactccagcg ttgccggact ggtctcgtat
     8221 gccggcactc cgatcctcac catcaacggc agcgcgtaaa aaggatagac cggatcattg
     8281 gacataaaga ccagctggtt catgtaaaat ccctggggga tgccggtcgt atcgacgctg
     8341 acagtgatcg gaacggctgg atcatcaggg aagatcaggc ctgatactga agccccgcca
     8401 ttgagggtga tccagttgct gggcagttgg gtggtgccgg tgatcgacag gtcgtctatg
     8461 tggatatcgt tgccatatgc gctgattccc aggagaccga cccgcacgtt ggcggcatga
     8521 ttcacgctgg agaggtccac gtagtgctgg tgccaacccc actgtccatt gtaacgagag
     8581 atcggcgcgc cgacgtccgt ccatgctcca ccagcgatgg atacctgcac ctggatccgg
     8641 tcgtcacggg tatcataacc gctgtcatgg aacatccaaa gcgacagcat cacgctggtc
     8701 ttgagcgaga ggtccagcac cgggctttcc agccggctgc tatttcccac attggaggtg
     8761 taggagttga aatatcccag ccaggagccg ctggagggtg gattgccgga gggatggacg
     8821 gttgagctgt aacgcagcca gtctccagcc gttccgctga ccggggtttc attccagccc
     8881 gcgggtggca tcgcgtcctc aaatccggtc tccaggatca catcgcggtc gcgcatgtaa
     8941 ttgacataac caaagtagct cagtgagtcc gttccggcat cagtgaggtt ggtaaggatg
     9001 cttccggact gtcctggcaa caagtcaaac tgcagctggg tgttgtccag aaacaactta
     9061 gcgcctaccc cgttgcccga gcagagcaga ttgaaggcag gttgatcggc gttatgggtc
     9121 actaccatgt aaccagagta tgggatcggt tcctggggag caaaagtaat catatagata
     9181 ccgctggttc caggaaggta tgaaaagccc atatcgttgc ggctgaattc ctgctcgctt
     9241 ttggcttccg ggatcaattc ccttgcttcc gcggtcctgg tacccacaaa actgacactg
     9301 aatccagagg gcatatggat atcccctcgc agaatggcac tccccggatt ggcgatcacc
     9361 atgtactggg aagaagtgct catccaggta acgtcgccaa acgcgaaagc tccggggttg
     9421 acctgaatct ccggaacgtc cgaagaccag ttgacgcggc caaagggatc gttttcatgg
     9481 ccggttccgg caaagccacc gctttctcca ttaaaggata cttcgccctg agcgagcgtc
     9541 ttgctcacgt tgaaggtctg gctgccgcca ctggggaagc gggcgtcttg tatctggaag
     9601 gtctgggcgt tgtcgatcgc gagcagagga cctccgctgg tcccaaaccg gaaggtgcag
     9661 tgatgcagag catgggtggg atcgaccaac gcgcctgagt tgatccggat gccgttgctg
     9721 ccgggatatt caaagatagt ccactctgat gacaaggtag cgccggagtt cacttccagg
     9781 ttgtaagttc cactcaagct ggttattaac gcctgagctt cgctggtgcc aagggtttcc
     9841 aggacgccac cgctgttgac actcagggag gaggaggaat acatggtcaa tatgcctccg
     9901 ggaccgatgg caagcttgcc gtcggagttg acattcaccg atcctttggt ctgcaatgaa
     9961 taagcaccca ggtccaaaca accgccgtct atcgttatgg tgccagacgt gttgagcgcg
    10021 atattggagc ccagactcaa tgaaacaaag gtggtccggg aggaggattt atcgatatgc
    10081 aggttcttga aactggccgt accgctgttg gatagaactc cgtttcctgt gccgacgaag
    10141 ctcacagtgc cggtgttgtc attccatatc ccgccggatg cgatgttcag attaccgccg
    10201 atcttgtgcg tgagcccgga agctccatag ccccaggtgc cggaagtgat ggtcgcgttg
    10261 cccttgaggt tcaccggctt ggctggctgg aagatgccgg agctcttgat taaaaacaga
    10321 ttgttgagcc cgatctcggc atatcccacc gctatggtct gattgccgct gccattcaga
    10381 atcaccgatc ccgaagcatt gatcaatccg agcttggggt tgctaccact gtaagtcata
    10441 ttgctgtctg agaggttgcc agccaggtag aaatcgcctc cagtcacttc tatataagcg
    10501 ttttgagcta tgcttacgtc agaattgatc tgcaaagttc cattcacacg caatccgccg
    10561 gagacgacat tgagcaggtt ttgaaccgtg ctattgccgc tgctgttcag gtattgggtg
    10621 cttaaaggat agtcaaccgt gactttatga aatgtctgag ccccgatcag tgagtggaga
    10681 ggcaaatagg catactgatc ccggaaaacc acttcgctgg taccggatgt gtatccggat
    10741 gatcccacgt tatttaccca atctcctcct attgtaagag catgtccgtt gctgttgaag
    10801 tttccgctcg taatcgtgat tgaacccgtg acgatcgcgg gggtggacaa cacgagcata
    10861 gaaccgggtt tattgattgt gacagacggt aatgtgttgt ttgctcccat gttgatattg
    10921 gcctgtacac taccgataaa ctcgatagtc ccgttgccgg ggtcaaacgc gtcatcgctg
    10981 acggcataaa accccctgcc gatcctgatc acggaaaatc catctgtgac ccattgagcg
    11041 ccactcacaa attgcagccc tttgttgggg aattcaatcg tgctgccatt ggtggtccag
    11101 gcgcaattgg caatctgcca ggttccggta tagggggcgt cgttgacaaa ggtcccctcc
    11161 ctggttttga acaaggacgt ggccgtggtg ctaaaattgc cgtgcacaag catcgccgct
    11221 cttctgacgt ccagctctcc acccgtcata gccagatcac cgtcgatcag gaaggtgtcc
    11281 agcgcaatgg cggcattggt ggtctgggat ttcaggatgc ctccgttgat cagcaggttg
    11341 ccgtggatat gagtgtcagt gggatccacc gtgcggttga aatccagatc gaccacgctg
    11401 ttcgccccgc tgatgctcat attgccaagc acgttgaggt gactgccgtc gagtatcctg
    11461 agctggccgg aacttatttc cagatcagca tttatgctca aagtcatgtc cgcagccaga
    11521 atcaacctgg cagaggggct gctgtcaatc ctgagatggt taaaggtctc cggggagccg
    11581 atggtcgtgg ttaatccacc tccgatgaag gtcaccagtc cactgccttc gatgaagttc
    11641 tccggggacg cgttgtttgt ccagctgcca ccaaaacgga tatgatcggt gctattgtag
    11701 gcatagagat cgccaccctg gatgacaatg tcctgctcca cagtaagggt gcgctcgttg
    11761 acatagagcc tgccgttttg caccgtgcag ctctttttaa cagtgaggtc ggtaagcaga
    11821 tagaccgctc cggtgctgcc ggccttgtcg atcacgatat tgggcagata gtttcccgcc
    11881 gccaggttga tgtttgatga acgctctcca atgaactgca ccgtgccgct gctggcctga
    11941 aagacgcttt cgctgagcgc gcgcaggccc cagcccagct tgagggttcc ggacccgctc
    12001 atgttgaagc tggagccgat gccaaactgg atgccgttgt tggtgatctg gaagttgcct
    12061 ccgctcaggg tcgtcgttcc ggcaaagctg taatgcgatc cgccgtaggg cgtatcgatc
    12121 ctgaaggtgc cgccggtgat ctggttggtc ccggtggcat attggatgta gttgttattc
    12181 acatagacca ggccgttaga ttggttgtaa aggcctgagc agtagaagtt ataagcgctg
    12241 atttccgcgt tgttgttcac agtcaggcag ccgccctccc agatatcgat cttaccctcc
    12301 acgatcatgg cgttgttgta ggggttgaac gtgctgccgc catacaccac cacatcattg
    12361 gcataaaaga gctttatcat gcctgtcggg tccagtgtgc aggaagagcc ggtcttctgc
    12421 accaggatgt ttcccagggt gagggttgaa agacctgtgc tcagagccga ggggctggtg
    12481 ccgtaaaact tgagaatgga agcatctccc atcgtaaaac tggtgctgtt gttcacgttc
    12541 atattgccct tgatggccaa agtcccggat acaatatcgc aggtggaagc gctggaccag
    12601 ataacgctgc cttccacgtt gacaatatcc gccaccgagt tcatcgcgag cgtgccggtt
    12661 atggtcagat taccggctat ccccaaggtg cagtcctgga tctccagcct tcccgtggtt
    12721 atggtcgcgt tcccgcccac aacggtatta ccatttatgt aaacggtgtt tgatgggctt
    12781 tttgccacgt tgagatggtg gattctggag ggagcgttga ctatcacgct tgagttcagc
    12841 cccccaaagg gctcaactgt cccgccggtc atcacaaatc caccccgggt gatcaacaag
    12901 cttcctgccg tgcggatcgt tcctccgtat aggttggagg tcaaagcata gcagccggat
    12961 tcatatacca tgccccgaat ccccagctcc agcacacctc cacccatcgt gaaattcaca
    13021 ctgcgggaag cggccaggtt acatcggcta cccccaacga ttgtaaatgt gccaccggtg
    13081 atagtgatgt ccgcgttgat atctaagctg ttgtcgccat cctgggccag attgatagct
    13141 ccggcgctaa gcgtgaaagt gccggcaatg tgaggatcat agaggtcgga tgccgaaaag
    13201 ctgcctccgc tcacgatgat cctgcccgaa gtccagtcat attgctggca ggagaccact
    13261 ttgcctgtcc ccagcagcaa cgtcccgctt ccggtcttgg ccagctccag gatctgggca
    13321 tcgagctgtt ggacttctga gatcccagac cccgcaaaga tcacccggtt gtctgttcca
    13381 ttcagagtac cggtgcctgt ccagcttccc caaaccgtca ttgcataacc gccggcgttg
    13441 atggtacctg aaaccgatac atctccccgg atgtttagcg ctgactgcag attgagctgt
    13501 gcgctgccgt tgatccacag attgttgata tagctgctgc cgctcatgga gatggtttgg
    13561 gctgtgctgc caatcgcttt cagcgtgccg gcctcttgtc tgaaatagcc gctacccagc
    13621 atcgagccgg caatttggag gtcggaatcc gcattttgca caaatccccc tccggctgcc
    13681 aagctgaaag tcccgttgac ctgcagccgg gccagcgagc tttggtagta aacagctttg
    13741 ttgtaaatct tggaaacata ggcatgttta agatttacca gcgcgtcaca ttgcaactcg
    13801 gcatcctcag ccccgtaaaa tgagacggtg gattgatcga gattgatggt gcaaccgctg
    13861 tccaccttcc aactgccatt gaatttcagc atccccgcgg ccgaatcgat tatactggag
    13921 ccagaatagc tattggtatg gcccttaaaa tctaccgtgc cagcgctggc acagagctgt
    13981 atctggccgc tcagatgggc ggtcccgtgc acgttcagag tccctggacc gaccttgatg
    14041 atccctccgt tggcaagggt gagcttttta cagctcccgg tcccgcctcc gttcagattg
    14101 ggatactgag cacatccgga agggattatg acgtcgtccg aggatcctgg cacgccttga
    14161 gtccaattcc aggcattggt ccaactctcg ttgatttcgc cagtccatgt attgggtgct
    14221 attgtgttga tagtgaaact gatagaatta cccgccgagc tacccacatt cgtgatcacg
    14281 atgtctccgg ctatcgtgct cagcgtgtcc gcgtagagcc atggactggg attgctgccg
    14341 ttgaaaatct ctgtcctgcc actttcctgg gagaaaaaag cgttacctgg ctggccattt
    14401 tcggtaagcg tgccattggg gcgatagacg taaatctcgt caggaggacc accggcattg
    14461 ccattgatcc cagtcgtcac ccgatatacg atcagaccgg agccatccag gttgttttca
    14521 tacaagcctg cttttttacg gtattccacc atgtaatact gatctggcac cgcggagatg
    14581 atcttgtagc agctataagg tgattcatta acggaagaga ttgtgtatgt tgtaggattg
    14641 gccgtggctg tgagggtcgg aatggcatca aaccaatgcc cgtacttcca tttgttgtgg
    14701 gtgagccagt gctgggccct gtcgctggcc atcagacacc aggttgagac gggatggatg
    14761 gaatgccatg gttcccactc atgatagtgg tagaaatccg gcgctccaat ctgatggccc
    14821 atttcgtgac agatcacact gatgcccaga gattgctgaa agttgaggat gacgctgtgc
    14881 acgtccttgc cgttgatagc atgaaacggg gtaccgctgg agttgctggt tggccacagc
    14941 agatctcccc aggcatcggt tgggccgcat acgatcagag tcaccccatc caccctgccg
    15001 tcgttgtcca tatcgatatt caagtcattc ggcaccatcg tgtccagctg gtccaccaca
    15061 tattcaaaca gctcgcgttt tcggggcccg ccctctccct cgggatagcc gatgtcattc
    15121 acgtcttcta tatagggaga aaaatagccc ctttcccacg gcaccaggat ggaaactacc
    15181 tggccgcttc cgttgggacc gggatagaga aagctgttta tcgtcagttg cccactggag
    15241 acctcgttgt aataatcctt cagcgacgcg gtatccatga ggttaaacat atcgctgtac
    15301 tggctgtagg tctttgaagt cggaaactcg tcctgctctt cgaagcgcac aaaagccaca
    15361 atgctgttga tcgtaccaat cggattggca cggtagatct gccgtaagcg ctgctcttcc
    15421 tcaaagcggg cccggaccgg agcttcctga ggcatcagac ctggcctgat ccccaacagg
    15481 gagggatcca ccctgccggc tatgtggctg gagggcatga tgccgtccaa gcctggaacg
    15541 gcatataccg cgtaacccgt cccgggctgc agaaggatgg tgaaacccgc cttgtcgtgg
    15601 acgcggtgat agtattcgtc cccggtgata taagccatga acttactgcc atcgggctga
    15661 acaatctcat aggcctggtt ttccacatag tttgccacgc cttgagaagc gcataaaatc
    15721 acaaataaaa gcactattga tcttcgcatg ggaacctccc tggcatttga cttttttatt
    15781 tgtctcctta actccagaca ccccacatgg acggccaccc cggatatcta ttatctgtta
    15841 agaccgttga acaaccagcg gtacgtccgc gtgaaactgt cttctattgc tgagtcaatc
    15901 atcactcccc agagctatag cgctgcagta taggggattc tatccagtaa catgattcca
    15961 gaatattatg acaaagtatt ggtgtcaagc gatatctgag atcataaact acaatttcat
    16021 gccttggctg tggattgttt gccattggca cccttcctgg gcgcctccga actgctcttt
    16081 ggcagtatcg acctgccgcg actgcagtca agcgacggtc aggtcacccc gtctgggctg
    16141 tttcccctgc cggttacttg ggatggaccg ccatttctat ttggccagca gcattttgcg
    16201 ggtacttgtg tgcctcgcgg tgctcaaccg gcagaaatag agcccgctgc tcaccatctg
    16261 gccccgggca tcagttccgt cccagattac gctgtggggt cctgatgcca attcggcatc
    16321 ggacaacaga atcttcacca gttggccctt gaggttatag acccccaggc tcacaggacc
    16381 ggtagcgggc aggctgaaac taatgctggt ggacgggttg aagggattgg gatagttgcc
    16441 cgtgagagtg ggatgactca caggagcgag ctgcggatcg tccgcggctg tgaacgcgct
    16501 ggtgaccata tttccccaga tgccggtcca ggcttcttcg ctgttgatga tcccggcccg
    16561 gtcgtcggcc caggccacca aagctctgtt tccaagcacc gccacgcgca cgtaatcctg
    16621 ttgataacgc tctgaacaca aagcaattgg gccatctcca cccgggatgc cttccggact
    16681 gatgtggcgg ataaagacgt cccggttctg aatccaggcc ccgtcgttat cgctgtaggc
    16741 gcagagcagg gagccgttgg caaacttgat cagcgtggcg tcatagcagt tggatgtctc
    16801 cgggaccacc actattccct gcagaggggt ggtttccagc ccctgggaat cgatcctgcg
    16861 caacacgatc gcctcgtcag gattggaggt gaggaaaatg atcccgtctt caaagatagc
    16921 cgggtagatg ttcatccagg tgtcggtccc accgaccaag gtgccgccct cgccccaaag
    16981 ccttgctccg gaggagctga gcctctgcac gcgtttacca tagagacccg gcctggacag
    17041 gctgataaat gccaccaggt cgtctccgat caggccggtg acatcataaa tctcgtcaat
    17101 atagtcgtgg gccgtgatca gattgacccc ggaggagtcc cagcccggag cgggatcgcc
    17161 attggcatcc actctcaaag ctttgcaatt gctgtaacca gttccggatg agtagacttc
    17221 ccacaagaaa tagctgcccg ccataccttt gaaaagcaag taatggttgg ccggagggct
    17281 gaagatcagc ttgccatact ggccccacag ggcctggccg ttcacgatcc tctggcctct
    17341 cagttcctgg ggctggccat atacagattt cacccagccg atcaggatgg cgtttccatc
    17401 gcagctcatt gaggccgctt cagtggtatc gacgctcaga gatagcccat taccaggata
    17461 ctccaggtta ccattggcat cgatcttctg cagataggtg gcataggtgg ccggctcggt
    17521 ggaatagtcc tcggtgctgt agataatggc cagcttgcct tccggagtcg tgacggcggc
    17581 attgacatta accacatcga agctggtgga aaggacggcc ctgccacccg gctccagaag
    17641 cgcctgggta ttctggttga tgatctgaaa gtagaggttg tcccggtccc gacggtcgat
    17701 ccagaggctc aggaagcggt ctgagaggct gtaagcaccc acaatgctgc agttgtagtt
    17761 gagccgctct tccagagtca ggcctccggg aggataactg aaggccccgt tcacgcttaa
    17821 cgcctgcagt ttgatagact tatagccgga ttccgtagtg ctccacaagt agaatcccag
    17881 ggtttgacca ccaaagctca gcacctctct ggccctggcc gagatcagtt tgcctcccgc
    17941 ttcccagata ggactcccat tggcatccag cacctgggct atgaagaaag gatcgtcgta
    18001 ataaccctgc caactggtcc agagtttgcc gtccgcgccg gagtttatct ggatgctgct
    18061 gatattggag tcggagccgg ctatagatac cggttgaggc cacactggcg ctagagcgga
    18121 ggacagcttt tggacaaata tctgatctcc gccttcccat tcagagccct tccaggcaat
    18181 tgccactccg ccctcgggag tgccggtcag tcctttaacg acggcatatt cggtgccgct
    18241 catcgcaaag gccacggcct ggggataaag caaagtccca gaggcatcta tcttgttcat
    18301 ataaaatatc ttggtgctgc cgtccgtctc ctgatagagc aggtactgcc cattccctaa
    18361 ggggaggatt tggtacaccg gtccggaaaa agtgccggcg ggaaccaggg accccgtccc
    18421 tatttgagat ccggtgggcg aaaagcgcag cagactggat ttccagaccg aattgatcca
    18481 gtattttacg ctcaggatag tgcctccgga gccgtccgaa accgcggcgt cgaggtggat
    18541 atgtacttcg ttgtccacca gcagttgtcc cgccaggggc caaagccggt tgccaaagct
    18601 gtcgaagttc tggcccatga tatcctggtt gtaccagctc tgatagatca caaaggcacc
    18661 tcccagcgag tttgggacca aagccacctc aggcatatcg atgggcaggg agctcaccgg
    18721 caggccactg ccccaaagca attgaccgtt ggaggttacc ttctgcactt tcaactgagt
    18781 cacttcttcg atttcacgct ccgcccagag gatgatgaaa ttgttgtccg agctggccac
    18841 cacgctcatg aggtcctgat ctccaggggc cgtcaccaat ggtaccgcct cgtcaaacag
    18901 cacctgagcg ctgtcactga tcttctggca ccagatatcg cggtcgccgg agctcagatc
    18961 gctccaaaac accagcctcg atccatcggc agccttgaaa ctaaaggttt gcagctcaag
    19021 gtcatcggcc ttgcggatgg cgatctcctc ggcattcagg cccagcagta ccaacagcaa
    19081 cagacaggca gtcaacagtg tctttctcat catccactcc cttgtgattc gatctttaaa
    19141 aacaatgtta gcatatttca agccaattac gcaattattt actcttgtcc cctggataca
    19201 tgttgcaact tgatttacct gactaaccgc ctatcttgca tacgtctctg ccttcttccc
    19261 gcatcctatt acggactcat tacggactta gtccgtaatg agtc
//
LOCUS       DAOSFN010000030        19300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2298_length_19300_cov_9.532554, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000030 DAOSFN010000000
VERSION     DAOSFN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 19300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19300
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2298_length_19300_cov_9.532554"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PLO35_03360"
     CDS             complement(<1..1030)
                     /locus_tag="PLO35_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOH78734.1"
                     /translation="MNKEELLIAIKNPEWTGIEFKQCRYGVSDDAYKTVSAFANTSGG
                     YLVFGVKESTGKDGRDIVGVENVDKVQNDFLSCLRNGGRLSRVILSDAELHEIDGKHV
                     LVFHIPESDRKDKPVYLGKRIDQAYIRRGGGDEQCTESELRAFIRDASDAPYDRSLEH
                     GVDSSVFCNSSTLNWFRALLDKNHTGKYAHLSDLDFLLEMGLLVEEAGKAIPTKASIL
                     LFGQDRFVRQILSRPVVDYQRIDQLEDNWDADKRWHDRLVIESNLFEAWRVLIDKYSL
                     IADIPFSLDETSLQRKDDTLEFRSFREAAVNLLIHQDFGDASRKAEIKIFKDKTIFWN
                     PGSAYSSVE"
     gene            complement(1840..4161)
                     /locus_tag="PLO35_03365"
     CDS             complement(1840..4161)
                     /locus_tag="PLO35_03365"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOH78735.1"
                     /translation="MTLRKPLLVFMMAMLVACLAGVSMEDLDTALDQSYVPTVMSFTA
                     MDGSDSLAVVPPTADETAMGDALFIQFLEEDHPLGELELAAAKWQEANPEAYTAWIEA
                     KTSDPASPLKFFYLWFLRQDDPLSRINAARQLIENYPDQVYGYRLMTLGYFENYPLED
                     YFDEPEAVKEMLNLDMVHFMTYFHNFPDDEYHRLAGVWALNRGGRSDQAGDLLDEAFM
                     AKDPWMVEIDPTRLQPIEEFHDLLFTMAGIIVNQEAQEEWAGTLAEIKAELVPYYFED
                     ALNYERVYELLANDHASLDDYYNRFALVSSLFELERTEELPLYLVDPNDLTDTIAFQD
                     AWINYDPASAREVYFAALQDDGDPLSHYLLNRLADDKSQAIKDARDLIDDYPRGLYGY
                     QLLGETYWKYFTTAAPDDSLRAQWTKWLRKDNGRLRAYSIRFPEDVKAQSAYLLTQII
                     AGNADRAYKYYLKVLNQVPFSNEIRQVDKIMADAGQFDLLWNSKVAYIDKFIADGMLE
                     GADREMYEVVAYCSALYSNGYYAKVTETVYESPNWMDYEDIQYMMVNSHYQLGEYGEV
                     IDILRMMVDRKTIALEDLRGLEQQPIAAHENWPALMEYAAQLAGIELEPTEPAESTEL
                     DGPAESQGFEAYPAPEWILPDPEGNIISLKDLRGQILILDFWATWCGPCRSAMPLINE
                     WMATDMPEGVQVFSINVWEDDKQAVIKFMNDNKYAMHLLFGTEELTSLYGVEGIPFIC
                     VIDGEGMVRGVEKGYSPTLKDTLNAWVNEILGR"
     gene            4350..5213
                     /locus_tag="PLO35_03370"
     CDS             4350..5213
                     /locus_tag="PLO35_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78736.1"
                     /translation="MKLNKHFPSWIILIGLVAGAVGCALISARSVQQYALADNEISEA
                     SGLAASRKLPGILYTHNDSGGEPIVYAINSKGMLAARVRLAGIKNRDWEDIAVGPDPG
                     TQKQCVFVGEIGDNDAAHKSVFVYRFEEPELSDTLLVIAAPDKIEIVYEDGPRDAEAL
                     FVDPRSGDICIVSKRETEVGLYRVKYPYSLTSPNTAVKEATLPMSYVTAADISPNGKY
                     ILIKTYPGILRYKRGSSQSIAAALAGKPKELPYQPEPQGEAVAWASDGKSYFTLSESA
                     ANKPSALFQYE"
     gene            5378..6070
                     /locus_tag="PLO35_03375"
     CDS             5378..6070
                     /locus_tag="PLO35_03375"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOH78737.1"
                     /translation="MKGSTLLLTTLAVVAVAVTVINLLPEELWKRLPGAPDSLSAPDS
                     SKLDTLKTSSPAQERQTDIPYDFSSIDPVPQPAREDIRFSQPRSRSRYSIEIQKSGFK
                     LILNRDDKPFRIYNIAVGNQGSDKLDKADLITPLGNFHVVSIENSSEREPKRGRAYGP
                     WFIRLLTGHFQTASGKAWSGIGIQGSGKASEIGRSLATDCVILQDKDLIELKEYIDED
                     YKNTLLPVRIVP"
     gene            6024..6938
                     /locus_tag="PLO35_03380"
     CDS             6024..6938
                     /locus_tag="PLO35_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOH78738.1"
                     /translation="MKITKTPFFRCGLYPKRIGLARCLTAGGFIILMLVSLSACKAKK
                     PDEQRRYVVLSPEVAEIMAALDLGQDIVGLTDECTYPPEFKSIPKIGKFGMVRKETVL
                     KLEPQIVFTSGLEQDAITAELKKLKLEVVSVYPRSLEDMYAAIISIGDVTGRQERART
                     LVSELQASIKTTTDKSTRMIKPKVYIEINRDPLMSVSDQSFVGQLIEAAGGDNIFPTL
                     ERDYARVSAEDVIKAAPDIIICYSQDSLSNLIARKGWQEIPAIKNGLIYTEQNLDPDL
                     IQRAGPRARLGIKRLNDLFLAWGNDQRQ"
     gene            6935..7876
                     /locus_tag="PLO35_03385"
     CDS             6935..7876
                     /locus_tag="PLO35_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOH78739.1"
                     /translation="MRPLRLILPLLLGAAIAILYLSWGSPSPIILWQVRVPRMLLTLL
                     TGMVLGGVGSVYQLMLANPLAEPYILGISSGSALGSILLGVMGLGLMMPLGGFAGAML
                     TMVLVWGLAGHGGSFEKGRLLIGGVIAGMFFASGISLLMYLFHEDTIIILGTLMGNLG
                     RIFSINEWRVFLGLALACAAILYWLYRQSTTLDIVSSGDVFAGSVGIDVVALRKRVFV
                     LSSILIGIVVSYAGIIGFVGLVVPHVARFYMGSSQKRVYPFSMFLGAFFLLGCDLLAQ
                     HLTVLELPVGVITAAIGCPFFMWLMLRGGKSARSASF"
     gene            complement(7937..9682)
                     /locus_tag="PLO35_03390"
     CDS             complement(7937..9682)
                     /locus_tag="PLO35_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78740.1"
                     /translation="MKPRLPVIFLILLVLSACTTNKHQPSPQQAPSQLPTHVSMYFYS
                     AGSMQFYEGAYDTALNLFQRASSIDPGSVRIRRQLLLSAMYHYLQQRTDSLEVKKLMY
                     QNRDLIARDQELLDTAYSFYSETDDSEGIHWTIETMLDRFPSARAHVLNFITNYNQDS
                     IPNSALLVPALDLARNDPEELHALSQLFDMVDPPSALLAAKRLYEIDPGTDTAGWLAA
                     RIVAAGDQTAGAEYFSTLIYPQDLELMYLILDEALQTGQLQLMNQVSGQVIATKDSEL
                     SYLIALSALMSRDQQLLSTVESHYHDDVFVQQEQNYVTSLLIANSLLENETEDLGPML
                     DRLGSSQDLDNIVQFYILAQSSALQDPSAPVPDSIYSDLALKLGSRFKDTTRSRYLIS
                     TVLAIPDQDSTLSLNYSLAKEELIHSFIGQDIYTRDDLSWLLTAYYQKGLMGERVALL
                     RKGIELFPDEHAWYNDLGYTLLVQGGDREEAAALIFEALGMDPANHFYLDSIAWYYYL
                     EGDYLHAREFITGVMEMEDMPSEIAYHIGLIHLKLNDFATATSYMVRAAEASDDPTYN
                     DRARRALDLWGVRAE"
     gene            complement(9679..10740)
                     /locus_tag="PLO35_03395"
     CDS             complement(9679..10740)
                     /locus_tag="PLO35_03395"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOH78741.1"
                     /translation="MKYIPILIIALALLGGCGGGSGQSARYTAFDNIIDHSKPVALGE
                     DDDVYVFCGIENRSHLEPLLKESIEREVALVYQEKYFNLIFADIKDMSGISRYKNLLI
                     VGSLEGGDPVSRHIKETLSKDLQDRVSQSGGDIFISKNRFTRDQLIIHLVGLDDSRLK
                     ELASSQANRLFSAFLERYTQRLAHQAYLGKVIPASFFAPYPFSLKVPDTFQLFSNDRN
                     NRFLSFLYRARMQNREIPDKFISVYYEDMDTDRVDETWLTTQRSRIGKTHFEGDSLNV
                     ETLRSERFKFAGHDGYRLSGAWINPGRMAGGAFQSYAFWDSKTKKAWLVDTMVFFPAG
                     DKLPTLMELFMIASSFGTK"
     gene            complement(10737..11294)
                     /gene="pgsA"
                     /locus_tag="PLO35_03400"
     CDS             complement(10737..11294)
                     /gene="pgsA"
                     /locus_tag="PLO35_03400"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012360115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOH78742.1"
                     /translation="MRSLPNLLTMLRVLLIPWFLWLVFVSTHPGAIQTALFVFAAASI
                     TDWLDGYLARKWNVISDFGKIMDPLADKLLVLSALAGITWRLPFRLSMLIFSIILVRE
                     IVITILREVYKRRGIIVPADKLGKIKTVMQMTGLVAAYALWAWMAEISPLARSLVNIW
                     FWLVAVITVVSGANYLMAKAPGAKK"
     gene            complement(11287..12129)
                     /locus_tag="PLO35_03405"
     CDS             complement(11287..12129)
                     /locus_tag="PLO35_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78743.1"
                     /translation="MKTRFFLMTALICLLLVAWVWLEQQYENARLEKYRQLSDLPVYI
                     YVADSSRVAPLMERLAEFPQLRDINLETGLQAADELVQAYQLPISTGSLADYRFPIVI
                     TLLFKPLQASVEVKPAVMRAIAEQQIELTDIDAQSNAWGQTVKELAALRQRWLSYTII
                     TAVMLGLLFFFIRLSYEFRFLLLQKRRLLSVADVLRVSTENHRHTWAMLVLPLLITVG
                     GYFLLDLSGYARAIIPLWWFAIPAGTLLAATAVVFIVLNMYFHDRTFMPETRPDAAEE
                     DSFA"
     gene            complement(12133..12645)
                     /locus_tag="PLO35_03410"
     CDS             complement(12133..12645)
                     /locus_tag="PLO35_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOH78744.1"
                     /translation="MEDRFDHGPLRVLDLACGSGIVAVMLGLQRPAWNISGLDIQAHL
                     IGLAQSNAANLGLRIDFFEADLRSFRAAEAYELIVANPPWLKAGSGLASLDIARELSR
                     REISCTAADVMSCLKRNLQPGGEAVLVYPASRLDELKSLAGNYLLDIIQALPTAETNK
                     YLIYHFKLRG"
     gene            complement(12747..13643)
                     /gene="nadA"
                     /locus_tag="PLO35_03415"
     CDS             complement(12747..13643)
                     /gene="nadA"
                     /locus_tag="PLO35_03415"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOH78745.1"
                     /translation="MINEINSLRREKNAIILAHNYQTTDIIELADFVGDSLQLSIEAS
                     KVEADFIIFCGVRFMAETAAMLNPRTKVLMPALDAGCPMADMVTADQLKKFREEHPTA
                     AIVCYVNSSAEVKAESDICCTSSNAVKVLQSLPKDQAVLFVPDQNLGSWAARQAGREI
                     ILWDGFCPIHHWGFEIAQVKALRKKYPDHRLIAHPECDEGIVEAADAVMSTGGMLAEA
                     KHHDQLIIATEKGLVDYLIKLYPAKSIIHLNTKAICKNMKKTTLAHVLDALRFEQHQV
                     WVDPEVARRAVQSLQRMLELSN"
     gene            complement(13762..14196)
                     /gene="dut"
                     /locus_tag="PLO35_03420"
     CDS             complement(13762..14196)
                     /gene="dut"
                     /locus_tag="PLO35_03420"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOH78746.1"
                     /translation="MKVRYRLLSVSAIAPQRMTEHSAGYDLCADLADPLLVPPGKRVL
                     VPTGIAIEVPAGHEAQIRPRSGLAAKLGLGVLNSPGTIDPDYRGELKVILINHGEDEV
                     IVHPGMRIAQMVICPVLSCHLIESEELTDTERGEGGFGHTGH"
     gene            complement(14193..15083)
                     /locus_tag="PLO35_03425"
     CDS             complement(14193..15083)
                     /locus_tag="PLO35_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOH78747.1"
                     /translation="MDRKVLLKKDMKEKQELVNIILDRYLPRKDEYPKNLHKAIRYSV
                     FAGGKRLRPYLTMLAYQIFAGNPEPITPVAAAIEMIHTFSLIHDDLPDIDNDDYRRGK
                     KSCHALFGEGEALLAGDSLLVSAFELITYAEISAELRVRFIRELARECGISGLIAGQM
                     VDIQSEGKKVSKKTLQYIHENKTAKLINICLRFGALAGGAPPEELAAIEEYGNCLGMV
                     FQIVDDLLDIEGDPEELGKSVGKDVEVEKATFPSVWGVEESRRQARELTEKAKQAIVK
                     LGEKAMLLDVLADYMLTRKS"
     gene            complement(15083..15343)
                     /gene="xseB"
                     /locus_tag="PLO35_03430"
     CDS             complement(15083..15343)
                     /gene="xseB"
                     /locus_tag="PLO35_03430"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003620234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOH78748.1"
                     /translation="MNHSLNPAELSFEAALKELEAILERLTNPNEALTDLIKIYEEGV
                     IYLNICKLRLNEAEAQITILNDKLAKEGMETSFHGNDREVTE"
     gene            complement(15400..16044)
                     /locus_tag="PLO35_03435"
     CDS             complement(15400..16044)
                     /locus_tag="PLO35_03435"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOH78749.1"
                     /translation="MKPPSKAWLITPQAPPESWKGYQGISPEDLIIAVDGGLKRCLEL
                     AWEPHYLCGDLDSVDEGLIASYPTGQIWRFPREKNETDTELALRKCMELGIRDLLICN
                     DLSGRADHALGLVQNLLLAHKQGLQAKILTGGQELFFLPPLWEITGRKGCLLSLIAWE
                     GEARFEASEGLKWDLSGLALRTELSRGISNEITSETVSIRLASGLALAVLTEIG"
     gene            complement(16041..17042)
                     /locus_tag="PLO35_03440"
     CDS             complement(16041..17042)
                     /locus_tag="PLO35_03440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH78750.1"
                     /translation="MTSEEYQSASPETETAVPAEEQGPGELIPQPLIFPDRRWKISLQ
                     VGDIKRGIRVPMVVPVLAGLFLLFMFIATLILLFGGSGSGADARQVAALKAENARLRG
                     KLEGYAAQVDTIYKKVEALQASQESVKGQGGPEHPYVPARSNQTDQRSSRLTLESRIN
                     DIDNKLGYILGQIGPDLPLAMPGDQPNVPAPISGDGIPSVYPTFGEITSGFGLRIHPI
                     LDELEFHSGIDIANQIGTPVYATADGVVRHISYENGYGKRMFITHQDGYETQYAHLYS
                     YQVKEGDPVRKGQIIALMGNTGLSTGPHLHYEVIQNGDKLDPSPFLNRIDTDKFAGR"
     gene            complement(17237..17394)
                     /locus_tag="PLO35_03445"
     tRNA            complement(17237..17394)
                     /locus_tag="PLO35_03445"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17361..17363),aa:Lys,seq:ttt)
     assembly_gap    17283..17342
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17517..18974)
                     /locus_tag="PLO35_03450"
     CDS             complement(17517..18974)
                     /locus_tag="PLO35_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOH78751.1"
                     /translation="MNLKHLILIALALLGASGAAGLVFELPYSATHTKVLDFDLDGDS
                     DIILGCPVTGVDTLLVFYNDGHCNFTRVDYPVFDLMKQIDFVDLSGDDRPELVTQGRI
                     DDVPRALYYDNVNGVITDDYTIIHTFTHFPYEYISLIDADSDNDMDAVFCKRYQPSDG
                     GSFGICRNNGQGVFTDAFVTTTDRRISTYAVGHMDTDNWCEVILCTTTGVYQYHKVQN
                     QYQETLIDDTQWSSYYNCCYIDDFNNDGFNDILLHMYTGIAGFLAPYKIKLNNGDGTY
                     TDSGEFTFPDAAYVINQSDFNGDGYQDIIYLMMILEAPWVNSLYLVYNQHDGSFGDLH
                     VMEFGNCYSSSVRGAYLDGDNTLDLVFAGYHVGASTRMSILFNDGNGNFLDGPVSANE
                     DIVSEAGCVLDVYPNPCRDYVSFKTGETDTEELSINIFNIRGQLIDTLSASSGDQPIW
                     NLKDRNGKRVESGIYLLRVYEQGNYIGSRKLMVIK"
BASE COUNT         4304 a         5173 c         5171 g         4592 t
ORIGIN      
        1 cttcaacact ggaataggca gagccgggat tccagaatat ggtcttatcc ttgaagatct
       61 tgatctcggc ttttctggag gcatcgccaa agtcttggtg gattagcagg ttcactgctg
      121 cctcacgaaa agacctgaat tccaaagtgt cgtctttgcg ctgcaagctt gtctcatcca
      181 aggagaaagg gatgtctgca atcagggaat acttgtcgat gagcaccctc caggcctcga
      241 agaggttgct ctcaattacc agccggtcat gccagcgttt atcggcgtcc cagttatctt
      301 ccagttgatc aatacgctga taatccacta ctggcctgga aagaatctgc ctcacaaacc
      361 ggtcttgtcc aaacagtaat atagacgctt tggttgggat tgctttccct gcttcttcga
      421 ccagtagtcc catttccagc agaaagtcca gatcactcag atgagcatat ttgcctgtgt
      481 ggttcttatc cagaagtgcc cggaaccaat tcaaggtaga ggaattgcaa aatacacttg
      541 aatcaacgcc atgctccaaa cttctgtcat agggagcatc gctggcgtct cgaatgaaag
      601 ccctcagttc agactctgtg cattgttcat ccccaccacc tcttctgatg tatgcctggt
      661 caattctctt gcctagataa actggcttgt ccttgcggtc agattcaggg atatggaata
      721 ccaatacatg tttcccatct atctcgtgta gttcggcatc tgagagtatc accctactca
      781 gcctgcctcc gttgcgcagg cagctaagaa agtcattctg aaccttgtct acattttcta
      841 caccaacaat atcccgtccg tcttttccgg tgctttcttt gactccgaaa acaaggtagc
      901 ccccactggt attggcaaag gcagacacgg ttttataggc atcgtccgat acaccatatc
      961 ggcattgttt gaactcaatg ccagtccatt ccggattctt tatagctatc agcagctctt
     1021 ctttgttcat gttgaggctt actccaacac tatccttacc cgctgagggc tctttccctg
     1081 agagagcgag tcactgtatt gatctaagac ccgtttcaat ccagtagggg cgatcgatcc
     1141 tccagcctgc ttagcccaaa gccggtcttt ggtttgggca agtaacgcaa gggaacaaag
     1201 gaccaacgcg agtatattga gtagccttct catagatcgc tcctgaagtt ttcacagaat
     1261 atctgttgat ggagtgccac tagcaagggc ggtataaaca cacaccgcgg tcaaggctgt
     1321 gcttggctgg cctgctacaa gggattcctg tttcacggtt cctccagtaa agattaaaaa
     1381 gaaatcactg tttacatgtt tgaatatccc gataaagctg tcaacaaata tcggcgatcc
     1441 tgggaggaat atttttgctg ggactcggag cattatgcgt tacgggttat gcgttacgcg
     1501 gtttgcgtga tgcttcaccc aagcgtacac tcagttggcg cgaaaagcca acttggagtt
     1561 tattgcccct ggactgaatc ggcgtttatc tccttgcttg agggggcttt ggacttcaag
     1621 ctatatcgga gcaatcatgc ccgcggatgt gctggttgaa gtccaagcgg cccgtaaact
     1681 cgctgccata tatactctta gcgtggccgc ttgaactcca acgggtcaac ggcctcgctg
     1741 ttttagcgca agctcttcag ccggtcgtga tgctcacgct attagtaaac aaggggtgga
     1801 agttccaccc cttattacga tcaggataac ttatgctgtt tatcttccca ggatctcatt
     1861 tacccaggcg ttgagcgtgt ctttgagggt ggggctgtag cccttttcca ctccgcgcac
     1921 catgccttcg ccgtcgataa cgcagatgaa ggggatgcct tccacgccgt agaggctggt
     1981 cagttcttca gtgccaaaga gcaggtgcat ggcgtatttg ttgtcgttca taaacttgat
     2041 gacggcctgt ttgtcgtctt cccagacgtt gatggaaaat acttgcacgc cttcaggcat
     2101 gtctgtggcc atccattcgt tgatcagggg catggcgctg cggcagggcc cgcaccaggt
     2161 ggcccagaaa tccaggatca ggatctggcc gcgcaagtct ttgagggaga tgatgttgcc
     2221 ttcaggatca gggaggatcc attccggggc gggatacgct tcaaaaccct gggattcggc
     2281 aggcccgtca agctcagtag actcggcagg ttcggtaggc tcaagctcaa tgccggccag
     2341 ttgagcggca tattccatca gggcgggcca gttttcgtgg gccgcgatgg gttgttgttc
     2401 caggcctctg aggtcttcca gggcgatggt cttgcggtcc accatcatac gcaggatgtc
     2461 gatcacttcg ccatactcgc ccagctgata atgggaattc accatcatat actggatgtc
     2521 ttcataatcc atccaattgg ggctttcgta aacggtctcg gtgactttcg cgtaatagcc
     2581 gttggagtag agggcggagc aatacgccac cacttcatac atttcgcggt ccgcgccttc
     2641 cagcataccg tccgcgataa acttgtcgat ataggctacc ttgctgttcc acagcaggtc
     2701 aaattgaccg gcgtcggcca tgattttatc cacctggcgg atttcgttgg agaaaggcac
     2761 ctggttgagg actttgagat aatacttgta agcgcggtcg gcatttcccg cgatgatctg
     2821 agtgagcaga taggcggact gggctttgac gtcttccggg aagcggatgg agtaggccct
     2881 caggcggcca ttgtccttcc tgagccactt ggtccactgt gccctgaggc tgtcatcggg
     2941 cgcggcggtg gtgaaatact tccaataagt ctcacccagc aattgatagc cataaagccc
     3001 gcggggatag tcatctatga ggtcccgggc gtctttgatg gcctgggatt tatcatcagc
     3061 cagcctgttc agcagatagt gtgagagggg atccccatca tcctgaaggg ccgcgaaata
     3121 tacctcgcgg gcactggccg ggtcatagtt gatccaggca tcctggaagg cgatggtatc
     3181 cgtgagatcg tttgggtcca ccagatagag tggcaattct tccgtccgct ccagctcaaa
     3241 gaggcttgaa accagcgcga agcggttgta gtagtcatcc agagaggcat gatcattggc
     3301 caggagctcg taaactcttt catagttgag cgcgtcctca aagtagtagg gcaccagctc
     3361 cgctttgatc tcggcaaggg tgccagccca ttcttcctga gcttcctgat tgacgatgat
     3421 cccggccatg gtgaagagca gatcgtgaaa ctcttcgatg ggctgcagac gggtgggatc
     3481 gatctccacc atccagggat cctttgccat aaaagcctca tcgagcagat cgccggcctg
     3541 atcagacctg ccgccccggt tgagggccca gacacctgcc agacggtgat attcatcgtc
     3601 aggaaaattg tgaaaatagg tcatgaagtg gaccatgtcc agattcagca tctctttcac
     3661 ggcctcaggc tcgtcaaaat aatcctccag gggatagttt tcaaagtagc ccagcgtcat
     3721 aagacggtaa ccatagactt gatcgggata gttttcgatc agctggcggg cggcgttgat
     3781 cctggaaagg ggatcatcct gcctcagaaa ccagagatag aagaacttca agggggaagc
     3841 gggatcggag gttttggcct cgatccaggc agtgtaagct tccgggtttg cttcctgcca
     3901 tttggcggcg gccagttcca actcgcccag aggatggtcc tcttccaaaa actgaatgaa
     3961 gagcgcgtca cccatggcag tttcatctgc ggtgggcggg accacggcca ggctgtcaga
     4021 accgtccata gcggtgaagg acattacggt gggaacgtag ctctggtcca gcgctgtatc
     4081 caaatcttcc atcgagaccc cagccaggca tgctacaagc atcgccatca taaaaacgag
     4141 tagtggtttt ctcagtgtca tttttcctcc atgacaaata tctatgatca tatagagcta
     4201 tctattagac gcaaagccca gactctggac acgggcttag cccataaccg ttgccgggga
     4261 ctatatgtca agtacttttt cagcaaaaaa aagcagcggc ctccgctctt tttcttgacc
     4321 aaaagcgccc catctgaaag cgtggcctca tgaaattaaa caagcatttt ccaagttgga
     4381 tcatcctgat cgggttggtg gccggagcgg ttggctgcgc gctcatctca gcccgttccg
     4441 tgcaacaata cgctctggcg gacaatgaga tctctgaggc ctcaggtctg gctgccagcc
     4501 gcaaactgcc cgggattctg tatacccaca acgattcagg cggcgagccg atcgtgtatg
     4561 ccatcaactc gaaaggcatg ctggcggccc gtgtccggtt ggctgggatc aaaaaccgcg
     4621 attgggaaga cattgccgtg ggccctgacc ctgggaccca aaagcagtgc gtctttgtag
     4681 gggagatcgg agacaacgac gccgcccaca aatctgtctt tgtgtaccgt tttgaagagc
     4741 cggagctctc ggacaccctg ctcgtgatcg cggccccgga caagatcgag atcgtgtatg
     4801 aagatggccc ccgcgatgcc gaagctctgt ttgtggaccc ccgcagcggt gatatctgca
     4861 tcgtcagcaa acgtgaaacc gaggtaggcc tgtaccgggt aaaatatccc tattccctca
     4921 ccagtcccaa taccgcggtc aaagaagcca ccctgccaat gagctacgtc acggcggcgg
     4981 atatttctcc caacggcaag tatatcctga taaaaaccta ccctggaatc ctccgctata
     5041 aacgcgggtc ctcacaaagt atcgccgcag ccctggccgg caagcccaaa gagctgccct
     5101 accagcctga acctcagggg gaagcagtgg cctgggcttc agatggcaaa agctatttca
     5161 ccctcagcga aagtgccgcg aacaagccgt ccgcgctctt tcagtatgaa tagaggcccg
     5221 gtatcctgag ctccatgaca gccccagctt tgttgacagc atttaatgcc agccggcgct
     5281 cgctggtttg ggatcttcac ctatcactct ggccaggttt gggaaagtac cccaggcaga
     5341 tcttgccttg ccgtaaacgt actgaccggg aggagatgtg aaaggatcga ccctgctcct
     5401 gacaaccctg gcagtggtgg cagtggcggt aacggtgata aatctcctgc cggaagagct
     5461 gtggaagcgc ctacccggag ctccggacag tttgtctgcc ccggacagca gcaagctgga
     5521 tacgctcaag acctccagcc cggcccagga acgccagacc gacattcctt atgactttag
     5581 cagcatcgat ccggtccctc aacccgccag ggaagacatc cgtttttccc agccccggtc
     5641 ccggtccaga tacagcatcg agatccagaa aagcggattc aagctgatct tgaatcgtga
     5701 cgacaagccc tttagaatct acaatatcgc tgtgggaaac cagggttccg acaagctgga
     5761 caaagccgat ctgatcactc ctctgggcaa cttccatgtg gtcagcatcg aaaactcatc
     5821 agaacgtgaa ccaaagcggg gacgggctta cggcccctgg ttcattcgtc tgctgactgg
     5881 ccattttcag acagccagcg gcaaagcctg gagcgggatt ggcatccagg gcagcggcaa
     5941 ggcatccgag atcggacgca gcctggccac cgattgcgtg atcctgcagg acaaagatct
     6001 aatagaactc aaggaataca tagatgaaga ttacaaaaac acccttcttc cggtgcggat
     6061 tgtaccctaa gcggattgga ttggcccgct gcctgacagc cggcggattc attatcctga
     6121 tgctggtctc actgagtgcc tgtaaggcca aaaagcctga cgagcagaga cgctatgtgg
     6181 tcctttcccc tgaagtggcc gaaataatgg cagcactgga cctgggacag gacattgtgg
     6241 gcctcaccga tgagtgcacc tatcccccag agtttaaatc cattcccaag atcggcaaat
     6301 ttggcatggt gcgcaaagag accgtgctca agctggaacc gcagatcgtc ttcacttcgg
     6361 gactggagca ggatgccatc acggctgagc tcaagaagct gaagctggaa gtggtgagcg
     6421 tgtatccgcg ctctttggaa gatatgtatg ccgccatcat cagtatcggt gatgtgacag
     6481 gccgtcagga acgcgccagg accctggtct cggaactgca ggccagcatc aagaccacca
     6541 ccgacaaaag cacccgcatg atcaaaccca aagtatatat agagatcaac cgcgatcccc
     6601 tgatgagcgt gtccgatcaa tcctttgtgg gccagctgat cgaagccgcg gggggagaca
     6661 acatcttccc taccctggag cgcgattatg ccagagtcag tgctgaagac gtgatcaaag
     6721 ccgccccgga tatcatcatc tgctactctc aggacagcct ctccaatctg atcgcccgca
     6781 aaggctggca ggaaatcccc gccatcaaaa acggccttat ctataccgag caaaacctcg
     6841 atccggacct gattcagagg gctgggccac gtgcccgatt ggggatcaag cgactcaacg
     6901 atctattcct ggcctggggt aacgaccaga ggcaatgagg ccactgcggc tgatcctacc
     6961 gcttctgttg ggagcggcaa tcgcgatcct ctatctgagc tggggaagcc cgtccccgat
     7021 cattctctgg caggtgagag ttccccgcat gctgctgact ttgctgacgg ggatggtgct
     7081 ggggggagta ggttccgtct atcagctgat gctcgccaat cctctggccg aaccctatat
     7141 tttggggatt tcatcagggt ccgccctggg ctccatcctc ttgggagtga tgggtttggg
     7201 ccttatgatg cccctgggtg gatttgccgg ggctatgctg accatggtgc tggtctgggg
     7261 cctggcaggc catgggggca gctttgaaaa aggtcggctc ctgatcggag gagtgatcgc
     7321 cgggatgttt tttgccagcg ggatctcgct gttgatgtat ctctttcatg aagataccat
     7381 catcatcctg ggaacgctga tgggcaattt gggcagaatc ttcagcatca acgagtggag
     7441 agtgtttctg ggcctggctt tggcgtgtgc ggccatcctc tattggctct accgccaatc
     7501 caccactctg gacatcgtga gcagtggaga cgtctttgcc ggctcggtgg gtatagacgt
     7561 ggtggcattg cgcaaaaggg tgtttgtgct gagctccatc ctgattggga tcgtggtcag
     7621 ttatgccggg atcattggat tcgtgggact cgtggttcct catgtggcaa ggttttacat
     7681 gggttccagc cagaagcggg tctatccttt ctccatgttt ttgggggcct ttttcctgct
     7741 gggctgcgat ctgctggccc agcatctcac cgtgctggaa ttgccggtgg gagtgatcac
     7801 cgcggcgatc ggatgtccct tctttatgtg gctgatgctg agaggtggaa agtccgcccg
     7861 gagcgccagc ttctgatcca aagtcccacg gccggcatga tttcagccat gcgcggcagt
     7921 ctgtaaagcg aaaacgctat tcggctctga cgccccacag atccaaagcc ctgcgggcac
     7981 ggtcgttgta ggtgggatca tcactggctt cagccgccct gaccatatag ctcgtggccg
     8041 tggcaaaatc attgagcttg aggtggataa ggccgatgtg ataggcaatc tcggacggca
     8101 tatcctccat ttccatgacc ccggtgataa actcccgggc gtggagataa tcaccctcca
     8161 gataatagta ccaggctatg ctgtccagat agaaatgatt ggcggggtcc atgcccaggg
     8221 cctcaaaaat aagcgccgcg gcttcttccc tgtctccacc ctggaccagc aaggtatagc
     8281 cgagatcatt gtaccaggca tgctcatccg gaaagagctc tatccctttt ctgagcaatg
     8341 ccactctttc ccccatcagt cccttttgat aataggcggt caacagccag ctgaggtcat
     8401 cccgggtata gatatcctgc ccgataaagc tgtggatgag ctcttccttg gccaggctgt
     8461 agttgaggct cagggtggaa tcctgatctg gaattgccag gacggtgctg atcaggtatc
     8521 ggctcctggt ggtgtccttg aaacgtgagc ccagtttgag agcaagatcg ctgtagatag
     8581 aatccggtac cggcgcgctg ggatcctgca aagccgagct ctgggccagg atgtaaaact
     8641 gcacaatgtt gtccaggtcc tggctgctgc ccagacggtc cagcatcggc cccaggtcct
     8701 cagtctcgtt ttccagcagg gaattggcta tcagcaggga ggtcacgtag ttttgctcct
     8761 gctgaacaaa tacatcgtca tggtaatggg attccacggt ggacagcagc tgctggtctc
     8821 tggacatcag ggccgagagc gcgatcaaat agctgagttc ggaatctttg gtggcaatca
     8881 cctggccgga gacctggttc atcagctgca gttggcctgt ctgcagagct tcatccagga
     8941 tcaggtacat cagttccagg tcctggggat agatcagggt gctgaaatac tccgcgccgg
     9001 cagtctgatc tcccgcggcc acgattctgg cagccagcca gcctgccgta tccgtgccgg
     9061 gatctatctc atagagtctt ttggcggcca gtagagcgga gggagggtcc accatatcaa
     9121 agagctggct gagcgcgtgc agctcttcgg gatcgttacg tgccaggtcc agagcgggga
     9181 ccaggagagc ggaattaggg atcgagtcct gattgtaatt ggtgataaaa ttcagcacat
     9241 gggccctggc actgggaaat ctatccagca tggtttctat ggtccaatgg atgccctctg
     9301 aatcatccgt ttcgctgtaa aagctgtagg cggtgtccag gagctcctgg tcccgggcaa
     9361 tcaggtcccg gttctggtac atcagctttt tcacttccag gctgtcggtc ctttgctgca
     9421 gataatgata catggcgctg agcaagagct gacgtctgat cctgacactg ccgggatcga
     9481 tgctagaagc ccgttgaaag aggttgaggg ctgtgtcgta ggccccttcg taaaactgca
     9541 tggagcctgc cgagtagaaa tacatcgaga cgtgcgtggg cagctgcgag ggtgcctgct
     9601 ggggactggg ctgatgtttg ttggtggtac acgcgctcaa aaccagcaga atcaggaaga
     9661 tgactggcag acgcggtttc atttggtccc gaagctcgag gcgatcataa agagttccat
     9721 cagagtgggc agcttgtccc ccgccgggaa gaagaccatc gtgtccacca gccaggcctt
     9781 tttggttttg gaatcccaga aagcatagct ctgaaaggcc ccgcccgcca ttctgcccgg
     9841 attgatccag gcgccgctga gcctgtaacc gtcgtggccg gcaaacttga agcgctcgct
     9901 gcgtagggtc tccacgttga ggctgtctcc ctcaaagtga gtcttgccaa tacgactgcg
     9961 ttgagtggtg agccaggtct catccaccct gtcagtgtcc atgtcttcat aatagacgga
    10021 aatgaactta tctgggattt cccggttttg catcctggcc cggtagagaa agctgagaaa
    10081 gcggttgttg cggtcgttgg aaaatagctg gaaggtgtcc ggcaccttga ggctgaaggg
    10141 atagggggca aagaaactgg cagggatgac tttgccaagg taggcttgat gggccaggcg
    10201 ctgggtgtag cgctccagaa aggcgctgaa cagccggttg gcctggctgg aggccagttc
    10261 tttgagccgg gagtcgtcaa ggcccaccag gtggatgatt agctgatcgc gtgtgaagcg
    10321 gtttttgctg atgaagatat ctccaccgct ttggcttacc cggtcttgca gatccttgga
    10381 aagggtttcc ttgatgtgcc gcgatactgg atcgcctccc tccaggctgc ccacaatcag
    10441 caggttttta tagcgtgaaa tgccgctcat gtctttgata tcggcaaaga tgaggttaaa
    10501 gtatttctcc tgatagacca gcgccacttc gcgctctatg ctctctttga gcagcggttc
    10561 cagatgggag cggttttcta ttccgcaaaa cacgtagaca tcatcgtctt cacccagagc
    10621 tacaggcttg gagtggtcga taatgttgtc gaaagcggta tagcgggcgc tctgtcctga
    10681 gccacctcca cagcctccca agagggccag ggcgattatt aagatcggta tgtatttcat
    10741 ttcttagctc ccggagcttt agccattaga taattggccc cgctgaccac cgtgatcacg
    10801 gccaccagcc agaaccagat attgacgaga ctgcgcgcca aaggcgagat ctcagccatc
    10861 caggcccaga gcgcgtaggc cgctaccaag cctgtcattt gcatcacggt cttgatctta
    10921 ccgagcttgt cggcaggcac gatgatcccc cggcgcttgt aaacttcccg gaggatagtg
    10981 atcactatct cacggaccag gatgatggaa aagataagca tactcagcct gaatggcaaa
    11041 cgccaggtga tcccggccag agctgagagc accaatagct tatcggccag ggggtccatg
    11101 atcttgccaa aatcgctgat cacgttccac ttacgggcca gatagccatc caaccagtct
    11161 gtgatggagg ccgcggcgaa gacgaagagg gccgtctgaa tggccccggg gtgggtgctt
    11221 acaaacacca gccataaaaa ccaggggatg agcagcaccc tcagcatcgt aagaaggttt
    11281 ggcaggctac gcaaagctgt cttcctcagc agcatcgggc ctggtttcgg gcataaaggt
    11341 ccgatcgtga aaatacatgt tcagcacgat gaacaccacg gctgtggcgg ctaataaggt
    11401 cccggctgga atcgcgaacc accataaggg gatgatcgct ctggcgtaac cgctcaggtc
    11461 cagcaaaaag tatcccccca ccgtgatcag taagggcagt accagcattg cccaggtgtg
    11521 acgatggttt tcagtggaga ctctcagcac gtccgccact gaaagcaacc ggcgtttttg
    11581 caggagcaga aagcgaaact cgtaactgag acggataaag aaaaacagca atcccagcat
    11641 cacagccgtg atgatcgtat agctcaacca cctctgacgc agggcggcaa gctctttcac
    11701 agtctggccc caggcattgg actgggcgtc gatatcggtg agctcgatct gctgttccgc
    11761 gatggcacgc atcacagctg gcttgacctc taccgaggcc tgcaaaggtt tgaacaacag
    11821 cgtgatcaca atgggaaaac gatagtcggc cagagagccg gtgctgatgg gcagctgata
    11881 agcctggaca agctcgtccg cggcctgcag ccctgtctcc aggttgatat cacgcaattg
    11941 cggaaactct gccagtctct ccatcagggg agccactcta ctgctgtccg ccacatagat
    12001 atagacaggt aaatccgaca actgccggta cttttccagc ctggcattct catactgctg
    12061 ctccagccag acccacgcga ccagcagcaa gcagatcaac gcggtcatca aaaagaaacg
    12121 ggtcttcatg gctcatcccc tgagtttaaa gtgatagatc aaatatttgt ttgtttcagc
    12181 agttggcagg gcttgaatta tgtcaagcag atagttcccc gccagggatt tcaactcatc
    12241 cagacggctg gcgggataga ccagcacggc ctctccccca ggctgaaggt tgcgcttcag
    12301 acagctcatc acatctgccg ccgtgcagct gatctctctg cggctcagct ctctggcaat
    12361 gtctaaagac gccaatcctg atccggcctt cagccaggga ggattagcca cgatgagctc
    12421 gtaggcttcc gcagccctga aactacgcag gtcagcctca aagaaatcta tcctcaggcc
    12481 cagattggca gcgttgcttt gggccagacc aatcagatgg gcctggatat ccagacctga
    12541 gatattccag gcgggccgct gcaatcccag catgacagct acgattccgc tgccgcaagc
    12601 aaggtccagc accctgagcg gaccatggtc aaagcgatcc tccacatagg cttgcaaggc
    12661 tgccgcggca ctcgaaaccg gctgggcgcc ctcagcctgt cggaatgctg ctttgcccca
    12721 aggcaactta acagcctggc cctgattcag ttagacagct ccagcatgcg ctgcaggctt
    12781 tgcaccgcgc gcctggccac ctcagggtcc acccagacct gatgttgctc aaatctcagg
    12841 gcgtccagca cgtgggccag ggtggttttt ttcatgtttt tgcagatcgc tttggtgttg
    12901 aggtgaatga tgctcttggc gggataaagc ttgatcagat agtccaccag gcccttctcg
    12961 gtggcgatga tgagctggtc gtgatgtttc gcttcggcaa gcatgccgcc cgtggacatc
    13021 accgcgtcgg cagcttccac gatgccctcg tcgcattccg gatgggcgat cagcctgtga
    13081 tcgggatact ttttgcgcag ggctttcacc tgggcgatct cgaaacccca gtgatgaatg
    13141 gggcaaaaac catcccagag gattatctcc cggccagcct gtctggcagc ccaggagccc
    13201 agattttggt ccggaacgaa aagcacggcc tggtctttgg gcagagactg cagcactttc
    13261 acggcattgg aggaggtgca gcagatatcg ctttccgcct tcacttccgc gctggagttt
    13321 acataacaga cgatagctgc tgtcggatgc tcctcacgaa acttcttcaa ttggtcggcg
    13381 gtcaccatat ctgccatagg gcatccagca tccagggctg gcatcaacac tttggtccgc
    13441 ggattcagca tggcagccgt ttcagccata aaccgcactc cgcagaagat aatgaagtcc
    13501 gcctccacct tgctggcctc gatggaaagc tgcagcgaat cgcccacaaa gtcggccagc
    13561 tcgatgatat cggtggtctg gtagttgtgg gccaggataa tggcgttctt ttcccggcgc
    13621 aggctgttga tctcatttat cattttggcg ttcaattact gatctcctca tgtatcacgt
    13681 ctaaagctcg tgtcttttct actaaagaaa ataatcaaaa tctcgtggct aaccacgccg
    13741 cctcaaagct tcagaacctg ctcaatgacc ggtgtgtcca aatccgccct caccacgttc
    13801 cgtatccgtg agttcctcac tctcgatcag atgacaagag agtaccggac agatcaccat
    13861 ctgggcgatc cgcatgccgg gatgcactat cacttcatct tcaccgtggt tgatcaggat
    13921 gactttaagt tcgccacggt agtcaggatc gatggtgccg gggctgttca aaactcccag
    13981 tcccagtttg gcggccagtc cgctgcgggg cctgatctga gcttcgtgtc cggcgggaac
    14041 ttcgatggca atgccggtgg gaacaaggac ccgcttaccg ggtgggacca acagagggtc
    14101 agccaggtcc gcgcagagat cataaccagc gctgtgctcc gtcatacgtt gaggagcaat
    14161 ggccgacaca cttaacagcc gatagcggac cttcatgatt tgcgggtgag catatagtcc
    14221 gccagcacat cgagcagcat ggctttttct cccagtttga caatggcctg cttggccttt
    14281 tccgtgagtt ctctggcctg gcggcgggat tcttccactc cccacacgga agggaaggta
    14341 gctttttcca cctcgacgtc cttgccgacg cttttgccta gctcttccgg atcgccttcg
    14401 atatccagca ggtcgtccac gatctggaag accatgccca ggcagttgcc atactcttcg
    14461 atggcggcca attcttccgg tggagctcca cctgccaggg cgccaaagcg caggcagata
    14521 ttgatcagct tggcggtctt gttttcgtgg atgtattgca gcgtcttttt gctgactttt
    14581 ttgccttcgg actggatatc gaccatctga ccggctatga gaccggaaat accgcattca
    14641 cgggccagtt cacggatgaa gcggaccctg agctcggcac tgatctcagc gtaggtgatc
    14701 agctcaaagg cgctcaccag cagagaatct ccggccagca gggcttctcc ctctccaaac
    14761 aaggcgtggc agcttttttt accgcggcgg taatcatcgt tgtcaatatc agggaggtcg
    14821 tcatgaatca gggaaaaggt gtggatcatc tcgatcgctg ccgccacggg agtgatgggt
    14881 tccggattcc cggcaaagat ctggtaggcc agcatcgtga ggtagggccg cagacgtttt
    14941 ccgcccgcga acacactgta gcggatggct ttatgcaggt ttttgggata ctcgtccttg
    15001 cggggaagat agcggtccag gatgatgttg accagctcct gcttctcttt catgtccttt
    15061 ttgagcagta ctttcctgtc catcattccg tcacctccct gtcattaccg tggaaactgg
    15121 tttccatccc ttctttggcc agcttgtcgt tcagaatggt gatctgggcc tcagcttcgt
    15181 tgagcctgag cttgcagata ttcagataga ttacgccttc ctcatagatc ttgatcaggt
    15241 cagtcaaagc ctcgttggga ttggtcagcc tctccaggat cgcttccagc tctttgagag
    15301 cggcttcgaa actgagttcg gcagggttta gggagtggtt catcgttcct cgcaatactt
    15361 acttatagct gtcactatat gtaatatgag tgatttttgt caaccgattt ctgtgaggac
    15421 ggccaatgcc agtccactcg ccagccggat cgagacagtc tctgaggtga tctcgttgct
    15481 gatgccacgg ctcaattcag tcctcagggc cagtccggaa aggtcccatt tcagcccctc
    15541 ggaggcttca aacctggctt ccccctccca tgcgatcaga gaaaggaggc atcctttccg
    15601 gccagtgatc tcccagagcg gaggcagaaa aaacagctcc tgaccgccag tcaggatttt
    15661 tgcctgcaag ccctgtttat gagccagcag caagttttgg accagcccca gagcgtgatc
    15721 ggcccggccg ctcaggtcgt tgcagatcag caggtccctg atgcccaatt ccatacactt
    15781 ccgcagggcc agttccgtat ccgtttcgtt cttctcgcgt gggaagcgcc aaatctgccc
    15841 ggttggatag cttgcgatca gtccctcatc gacggaatcc agatctccgc agagatagtg
    15901 aggctcccaa gccagttcca gacagcgttt caggcctcca tccacggcaa tgatcaggtc
    15961 ctcaggactg ataccctgat agcctttcca gctttcggga ggggcctgag gtgtgatcag
    16021 ccaggccttg gaggggggct tcatcgtccc gcgaatttat cggtatcgat gcggttcaaa
    16081 aaggggctgg ggtcgagctt atcgccgttc tggatcactt cataatgcag atgcggcccg
    16141 gtggaaaggc ccgtgttgcc catcagagca atgatctggc ctttgcggac gggatcgcct
    16201 tccttcacct gataactgta aaggtgggcg tattgggtct cgtagccatc ctgatgggtg
    16261 atgaacatgc gtttgccata gccgttttca tagctgatat gcctcaccac tccatccgcg
    16321 gtggcataga ccggagtccc gatctgattg gcaatatcaa tcccggagtg aaattccagc
    16381 tcatccagaa tgggatggat tctcaagcca aatccgctgg tgatctctcc aaaggtggga
    16441 tagaccgagg ggatgccgtc tccgcttata ggggctggca cattcggttg gtctcctggc
    16501 atggccagag gaagatcagg gccgatctgg cccagaatgt agcccagttt gttgtctata
    16561 tcattgatgc ggctttccag cgtgagcctg gaagagcgtt gatcagtttg attggagcgg
    16621 gctggcacgt agggatgctc agggccgcct tggcccttga cgctctcctg gctggcctgc
    16681 agggcttcca cctttttata gatggtatcc acctgggcgg catagccttc cagcttgccc
    16741 cgcagcctgg cgttttcagc tttgagcgcg gccacttgtc tggcatccgc tccggaaccg
    16801 gagcctccga agagcaggat cagcgtggca atgaacataa acagcagaaa cagccccgcc
    16861 agcaccggta ccaccatggg cactcggatg ccgcgcttga tgtctcccac ctgcaggctg
    16921 atcttccagc gccggtccgg aaagatcaaa ggctggggaa tcagttcccc agggccttgt
    16981 tcctcagcgg ggaccgctgt ctctgtctct ggactggcag actgatattc ttctgatgtc
    17041 attcactgat ttccaatacg ttagtcgtta tgcgtgacgt ggtaatatat ttaattttgg
    17101 ccatgggata gccacaatgc tggcgtgatg attattcctc atggtgttgc gattgtgttg
    17161 caaattccga gccaaacatc atttacttgc cgttaagctg cgggagttgt atctcaaaac
    17221 caccccataa aaaaaatggc atcccgtagg ggaatcgaac ccctgttgcg tgactgagaa
    17281 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnaccaacga ctctctgctt aaaaggcaga tactctaccg ctgagttact ggacctgttt
    17401 ttgagtatgt tcgagcattg atttttgagg ccgtttttct gtcaagcaga aaatgctcag
    17461 gggcttatct aaactaatca ctggcccgtg gcgacgaaag atcgatcctg atcggcctat
    17521 ttgatcacca tcagtttacg tgatcctata tagtttcctt gctcatatac cctcaagaga
    17581 tagatgccgg attccaccct tttcccgttt ctgtctttca gattccatat tggctgatct
    17641 cccgaggagg cagacagcgt gtcgatcaat tgccccctga tattaaagat attgatgcta
    17701 agctcttctg tatcagtctc tccggtcttg aaactgacgt aatctctgca gggattgggg
    17761 taaacgtcaa gaacgcagcc tgcctctgac acaatgtcct cattggctga aaccggccca
    17821 tccaggaaat tgccattgcc gtcattgaag aggatgctca ttcgggtgct tgcacctacg
    17881 tgatatccgg caaagaccag atcaagggta ttatcaccat cgagatatgc gccccggacg
    17941 gaactgctgt agcaattgcc aaattccatc acatgcagat ctccaaagct gccatcatgt
    18001 tgattataaa ccagatagag gctgttcacc cagggtgctt ccaagatcat catcaggtat
    18061 atgatatctt gataaccgtc accattgaaa tcggattggt tgatcacgta ggccgcgtct
    18121 ggaaaagtga attccccgga atcggtatag gtgccatctc cgttattcag tttgatcttg
    18181 tatggcgcca aaaatcccgc tatgcctgtg tacatatgga gcagaatgtc gttgaacccg
    18241 tcattgttaa agtcatcgat gtagcagcaa ttgtagtaag agctccattg agtatcatct
    18301 atcagagtct cctgatactg gttctggact ttatgatatt gatacacgcc tgtggtggtg
    18361 cagaggatca cttcacacca attgtcggta tccatatgcc ccaccgcgta ggtcgagatc
    18421 cgtctgtcgg tcgtggtgac aaaagcgtcg gtaaacacgc cctgaccgtt gttcctgcaa
    18481 atgccaaaac ttccgccatc cgagggttga tagcgtttac aaaacaccgc gtccatatcg
    18541 ttgtcgctat cggcatcgat caaagagata tattcatagg gaaaatgcgt gaaagtgtgg
    18601 ataatcgtat aatcatcagt gattacgccg ttcacgttgt cgtaatataa tgccctggga
    18661 acgtcatcga tgcggccctg ggttaccagc tccggacgat cgtctcccga taaatccacg
    18721 aaatcaatct gtttcatcag atcaaagacc ggataatcca ccctggtgaa gttgcagtgc
    18781 ccgtcattat agaagaccag aagggtatca acacctgtga ccggacatcc caggatgata
    18841 tcgctatccc catccagatc aaaatccaaa actttggtat gcgtggcaga ataaggcaat
    18901 tcaaacacca gacccgctgc tccgctggcc ccaagcagcg ctagcgcgat caatatcagg
    18961 tgtttcaggt tcatggtttt cctcgctttc ttctgtggca tatcgtctga tcaaattcta
    19021 agccattgat tctcataata gttgaggcag tagataccgg ccttggattc atagtttctt
    19081 tggatgtttg cagtgtttat atagccgctt gtttcgtcaa ggtttcattg gcccaagttt
    19141 atttttttgc tgaatctata tcaaccctct ttaagactgt agaataattg taacactggc
    19201 ttcagagctt gtgcgaaaac tggcgaagcc aaaggctgac atagccccgt tggagttcaa
    19261 gcggccacga taaatgtatg catgtcaacg agtttgcggg
//
LOCUS       DAOSFN010000031        19105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2326_length_19105_cov_8.525302, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000031 DAOSFN010000000
VERSION     DAOSFN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 19105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19105
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2326_length_19105_cov_8.525302"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..>1305)
                     /locus_tag="PLO35_03455"
     CDS             complement(188..>1305)
                     /locus_tag="PLO35_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOH78752.1"
                     /translation="KTELTPRQSSIYAICMMLFVASMDGASLSRDLGLIWVFVETTTL
                     ASAMLISFEKHKQSIEAAWKYLFICSIGIALAFVGILLLIIAQPHSPSLFLDRIISNA
                     GSLNSFWLKISFVFILIGFGTKAGLAPMHFWLPDAHSEAPAPVSALLSGALLNTALLP
                     LLRMQQVMNISHLGTLASQLFLILGFASMFIAAVFIMKITNYKRLLAYSSIENMGIIL
                     IAFALGDLAGQAGLIHIMGHSLIKSAFFLTAGNVYFLFGKKDYNACGGLLGLHPASGW
                     LWLIAFMFIIGMPPSPLFFSELFIAFGFIKAGYIPALIVYFILLSAVAYGLGRAALGI
                     ATGTATERKHLPLSRILPQMALLALAVAVSFLVRSGL"
     assembly_gap    1306..1405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1422..2057)
                     /locus_tag="PLO35_03460"
     CDS             complement(1422..2057)
                     /locus_tag="PLO35_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78753.1"
                     /translation="MADILLVIFGVSLLAASITNMLSSIARILILQGLILFALTILKT
                     NELNTLEFIFVALETIVFKAVLIPWFITDTIRKNGITREVEPSVSNFFSLGSMTAIFA
                     FGFFIALWSGKSSPNIYPLHFGIAFSAILKGLFIIIANKKLITHLMGYLIMENGIFLL
                     SLAIGSEMPYIVSLGVSLDILLSILIAVLFINKIRNSFEDVDSDELSTLKD"
     gene            complement(2058..2975)
                     /locus_tag="PLO35_03465"
     CDS             complement(2058..2975)
                     /locus_tag="PLO35_03465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOH78754.1"
                     /translation="MIVNLVFILILPLWNMGLIAKTKAIWAGRKGPSLLQPWYNVAKL
                     LRKSEVHSHTAGFIFRVAPSLALAATLMAAMLVPFGASSAVFSFRGDFLIFMYLLALS
                     KGIMVLAAMEVGSSFEGMGASREISFTAFLEPAFFLTIGSMVYAGGFESMGDLFLRHT
                     VVLSNHWSVIIAVMTSIALFIMLLVEGARVPFDDPATHLELTMIHEVMVLDTSGVNLA
                     MVHYGAALKMQIFASLISYFLIPEGHSPLAITAIYLLVTTSLAILVGWVESLMPRYRM
                     NRNLELVVIPLSVSLLALAALIVRNLGGI"
     gene            complement(2972..>4434)
                     /locus_tag="PLO35_03470"
     CDS             complement(2972..>4434)
                     /locus_tag="PLO35_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOH78755.1"
                     /translation="AIYYLIMMHIGAAFLLLGFGLIYRQSGIFNYSGIEFQGAAKWLL
                     LIGFAFKAGFFPFYSWLPKAHPVAPSHLSGMMSGLMIKTGVFGIVYVLANSSWTMVEV
                     LVLTGVSLITAFNGIIHALAESHLKRSLAWSSIENIGIIGIGLSFWQLGLISSNSTMA
                     ALGFSGAMLHLINHSLFKPMLFYLSGNILLATHSLDLNTLGGLDKRMPRTAALFFLGT
                     MAISAMPVMNGFISEFAIFLSVLSGFNASDIGTAITGIVAGAIFAFVSALALIVFSKA
                     YSVAFSGSTRSSSADHASERRTGLLISPILLACLCLGLGIFGRAGLYLVYPLTGLAGI
                     DPNAILSLGHTLDQLSVILLIFITFATVVYLLKRRLGKVSKGPAWGCGYHRPDAKMQY
                     TGTAFIDPLAYFLKPLMHKSSDKSVLTGYFPQELSFEEEVHDYLDSGIFAWLTKGLRW
                     ILDLFDRIHNGKINSYITYLLGALIILLIWVLGVSK"
     assembly_gap    4435..4534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4638..5390)
                     /locus_tag="PLO35_03475"
     CDS             complement(4638..5390)
                     /locus_tag="PLO35_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOH78756.1"
                     /translation="MFELIKARIRHGWQAIPDPLHASIHPAFPGVPVLLGDHAEDFEA
                     AEQICPTHAIQNHHLDLGKCVFCGACERGFPETFSFEADFRLASSSREGLIIRWDEEL
                     NASSRPGVSPHPVMAKVCRNSLALRNVSAAGCNACELELGACSNVNFDMGRYGLDVVA
                     SPRHADALILTGPVSANMAMALRETWEAIPAPKVLILSGTCAISGGIFNGSKALSREW
                     LESITPALYIPGCPAHPLSIIHGLISMLGRNV"
     gene            complement(5400..6875)
                     /locus_tag="PLO35_03480"
     CDS             complement(5400..6875)
                     /locus_tag="PLO35_03480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOH78757.1"
                     /translation="METKSLTGNPLCLKPPSPASNADFFQAIRSSCAAGGYPLCYFGT
                     PGGKLLCVLKLPGSELAVTQTDFPADLSYPSLAKDIPAFHMFERELWEEYGIHPIGHP
                     WLKPLRFPGMEGLDHRDYPFFRSTSKELHEVAVGPVHAGVIEPGHFRFICHGERVEHL
                     EIQLGYQHRGVEKLFREGDLRHKSCLAEAIAGDTAVGHALAYCLLVEALAGVRLSPEV
                     HTVRMIALELERIGMHLADLSALCGDIAYLSGLNMFAALRTTVINSSLAICGSRYGKR
                     WLAPGGINYGISKLQNKVLRQTLEAVSQQIDRVARATFADPGVLNRFDSTGTLSRQAV
                     QELNMSGITAKAAGLARDARLDFPIFGDVISYPEPFAPLVQESGDVYARAWLRYQEAL
                     QSIAIIQYLLSTLPELKLQRIELPEPPQGALAIAVTEGWRGRIVHAARISSGWNTEFY
                     KIYDPSLHNWFGLALAVRGEGISDFPLCNKSFDLSYCGNDL"
     gene            complement(6958..7512)
                     /locus_tag="PLO35_03485"
     CDS             complement(6958..7512)
                     /locus_tag="PLO35_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78758.1"
                     /translation="MLILIASIGSACVMRPWQPLNGSGYGVEKHYAIVANDSLLIAVR
                     PLAYSGTAANVSSNFFSLWVQARNLSKRTVNLNRSSFGVISGGRQYDFVPLEYVLGSL
                     RSGYLIAEFEDPFNTDPLSPSNLERNNEKYNEAYFEVLNSHFSFGDLLPGGMKEGYLF
                     YNNDIGSARAFSVDIFGTRVDFAR"
     gene            complement(7619..10249)
                     /gene="gyrA"
                     /locus_tag="PLO35_03490"
     CDS             complement(7619..10249)
                     /gene="gyrA"
                     /locus_tag="PLO35_03490"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH78759.1"
                     /translation="MEDKTKIINIQIEEQLKQAYLDYSMSVIVSRALPDIRDGLKPSQ
                     RRIIYAMHELNLSPGGHFRKCAKIAGDTSGNYHPHGEQVVYPTLVRLAQPWIMRYPLI
                     DGQGNFGSIDGDPPAAMRYTEARLHKVTIEMLDELEKDTVDYKSNYDETRKEPTVFPA
                     RIPNLLINGSSGIAVGMATNIPPHNIGEVCDAMIALIDNPELESLDLRQYIKGPDFPT
                     GGFILGADGIRDYFETGRGRILNRGQADLETNDNEQEFIIIRSIPYQVTKTLLIERIV
                     DLVKEKKIEGISDIRDESGRDGMRLVIQVKRNHDGQVVRNLLYKYTQLQSTFGVINLC
                     LIDGVPQVVNMKDMLTNFIEFRHDVLLRRTLFELKNAEDRMHILEGLRIALENLDEVI
                     ATIRASQTPPEANEQLQLKFGLSEIQAKAILDMRLQRLTGLERDKIEAEYQELLITIA
                     RLRELVARRELRMELIKQETREIRDKFGDPRRTTILENYGGSLNIEDLIADELMVVTI
                     THAGYVKRLPVDTYRVQGRGGRGLNAANLKEDDFIQYVFVASTHSYILLFTDHGRCYW
                     LKVYEIPEAGRTARGKAIVNLVNFQPDEKIKAFVTVRGFHPQHNIVMCTRNGLIKKSA
                     LTAFSRPRSAGILAIKLVEGDELIDARISEGADDIILATRNGYCNRFNEKDFRATARF
                     TKGVRGIRLRENDYVISMTVISQDDMLENGETPTRTLLAVSENGFGKRTTISAYPTTR
                     RGSKGVITLKTTIRNGHLAALMLVDQSEELMIITQEGMIIRQNVSEISEISRNTQGVK
                     LIRLNDNDKVRDITNVPNEEDENVIEKSVEKIKSAPPIPTVIPASDEDEIEDLDDQEE
                     EIDDLDAEDEIDEPIEDENE"
     gene            complement(10445..12343)
                     /gene="gyrB"
                     /locus_tag="PLO35_03495"
     CDS             complement(10445..12343)
                     /gene="gyrB"
                     /locus_tag="PLO35_03495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOH78760.1"
                     /translation="MIDQNYNANNIKVLKGLEAVRKRPAMYIGGTSERGLHHLVYEVV
                     DNAIDEALAGYCDLIKVTITPEGVVEVDDNGRGIPVDMHPEMKIPAVQVVMTVLHAGG
                     KFDDKSYKVSGGLHGVGVSVVVALSEFLEVRIHKNGKIHYQRYERAKPVTDLQVLGDT
                     NRTGTVIRFKPDDTIFETVEFSFDYLTTRLRELAFLNRGVRIMLKDERNDRLHDFKYD
                     GGIVSFVEYLNQNKKPLGVKPILMEGSKEGMDVEVSLQYNEGYQENIFSFANNINTIE
                     GGTHLSGFKTGLTRAVNNYIKSADLLKNEKTSPTGEDIREGLTAVVSVKISDPQFEGQ
                     TKTKLQNSDVEGFVSSMVLDKLTTYFEEHPSEAKTVTMKSILAARSREAARKARELTR
                     RKSVLESGSLPGKLADCSISDPTKTELFLVEGDSAGGSAKQGRERSIQAILPLWGKML
                     NTEKARVDKVLNNDKIQPIILAIGAGIGQDFDVTKIRYHKIIIMADADVDGAHICTLL
                     MTFFYRYMRPLIEHGHVYIAMPPLFLVRKGKQKKYVFTEAERDQAVQEFGDKGVVIQR
                     YKGLGEMNPEQLWETTMDPSVRSLMSIKMDDAIEADRMFTILMGDEVEPRRDFIQANA
                     RYVKNLDI"
     gene            complement(12885..14807)
                     /locus_tag="PLO35_03500"
     CDS             complement(12885..14807)
                     /locus_tag="PLO35_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH78761.1"
                     /translation="MKKAYLVILLLIGLVSIFAIQIGMKGERYATEGLEYFHRGEYNK
                     AIERFKEADLAANGDVPYYFYWLGRLHIAVQDTTNAMVWLDRYVKSEDQEFRQQVDTF
                     IEIIGNQDKAFEKISLRAMPAYINSRNSDYGAVVDPAGKYLYFTSWRPGKADKENIWR
                     AEIFKSGYGKPQLVTDLSTDSNEAMGSFAADSVGAYVFGNYEKGKLDGDIYYTEWDGS
                     RWTKPSPQPWFNSPDVDTHPSVYNDSLMFFSSSRPGGYGEIDIWVSQKVDGVWTEPVN
                     AGPNINTPGIEQTPHLVHFTKDIVHEGRNVEYKETALYFSSNTHPGFGGFDLFKAVHK
                     GPTWQDWHLAQNLGLPINSIQDDRYFSTRPGSNEVYISSDRQAPGFEKVLLAFVDYTV
                     PGYFVTQDTTGTRVYEVNIPPYVPPADSLGEPIIEEPVVEEPPRMITFTGKVTDEKGE
                     PVATDITFTGLVDDETRKDVVTTNEDGTFEITVPYADPWTVVINPEGFMLYQQDIPAP
                     PEGQDAVNIDFAIERLVVKKVFVFNNIQFDFDKATLRKESFPILDDIVITMLNNPEIK
                     IEISGHTCNIGTAKYNQGLSQRRAKAVVDYLIKKGVEKERLSYKGFGLSKPLNKNKTK
                     AQRALNRRVEVKVVEE"
     gene            complement(14822..15787)
                     /locus_tag="PLO35_03505"
     CDS             complement(14822..15787)
                     /locus_tag="PLO35_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78762.1"
                     /translation="MKRKHRNTKVLLLAALLGILLTPLMAGPLNNSAGAYMRLGIGAR
                     EIAMGEAGSAISRDVAAGYWNPAGLNKIKDIEVGVMYNFSMNLDRSHQYASIAKPFDF
                     GTLAFNWINAGVGDIEGYDNAGNPTGTFSDNQHNISVSYANHWKDLDYGVTPKFYFST
                     LDGETKAGMGIDLGARYDVNQYLVAGLMLRDLLGGYAGDRVPIEMAMGVAAYPLIGVT
                     LAADLKWEQRENPYLAVGAEYWTSIGKDPEADSKLSVISVKERSAWEDVFSYAQTGLR
                     LGFNQGHFSAGTGIRFRNFQLDYVFRLNNHEIMSDDHIISMIFRF"
     gene            complement(15799..>19105)
                     /locus_tag="PLO35_03510"
     CDS             complement(15799..>19105)
                     /locus_tag="PLO35_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH78763.1"
                     /translation="SATYAIGSYVPVVATPVFYPLPGSFTAPVSVSIRTDTPEATIRY
                     TTDGSDPTPASDPYLGPINVPNHTVMTIKAIAYKDEWDPSQIAVGTYTVTGTVQDVVF
                     NPLGGSYTTAQNVVITTPTPGAAIFYTLDGSDPTESSSQYTQAVQLGLNSTTTIKARA
                     YVNGWTPSAIGSQTYVITGQVTIPGQVFTPVAGTYTTAQPVTISTTTLPAGATIRYTE
                     NGTDPTATSPIYTGQTISVPLNTVKTVKARAFLAGWDPSPVYEAVYNVTGTIVLPAAT
                     FTPVAGTYQTAQNITINPPAIPSGASLYYTLNGIDPTEASLLYQGPIALPENSGPTTI
                     KVRAYKTGWVPSAIASATYTITGTVAYMTPVFLPDPTVIYTSPVNVTINNVTPTDAIR
                     RYTLDGSEPSLTNGFTYPTTPINLALNTSTTIKTKAFKTDWTASQTYTATYTVTGQVS
                     ITAPVFTPPPGTFINPQSISVSNTTVPAGAVVRYTLDGSEPTATSPIYSAAIPAPAYA
                     TTRIKVKAFATNWQASPTYEGVYVINGAAIINGPVFTPPAGTYTTAQSLVISNNTLPS
                     NASIYYTTDGSDPTTTNGTLYTDAIPLNLDQQITVKVRAYGDANWDPSIVHSASYNMT
                     GQVIIPPLVFSPNPGVVYQTATLVTIGAPVPSSATIHYTTDGSVPTQASAVYSAPIEV
                     PLNTLMTIKARAYLASWDPSPVYEATYAVTGQVQLAASPFSPAPGTYTSAQTVTIAAP
                     VLPVQGAILRYTLTPDLADPADALDPDSTATQYTGPITVPLNSNWVIKIRGFARDWID
                     SEVIAVRYVVTGNVEAPDFYQNSEPITAELFHDPLTIELRSATEGAMIYYTVAVGSDP
                     AIPDETATLFDPAQPIVIPELSFDVRIRAKAFKTDWIPSSDVIHSFTVIPLPYDVRAF
                     TYGGYIRLLWNFESGKALDGFDIYRRKTSEAAWTKQNTTPVLTMLGTDYYYDDYAIQA
                     GTSYVYRVVAIYNGVESNPSETTTIEYQSGELDISPATYAYPNPAGSSTKIKLVLTRN
                     DNVQVAVSIYDFSGKKVRTLTVPPTNSNLIEIPWDLNNSSHKKVGRGTYFARIVANDG
                     VKKAERTIKISVK"
BASE COUNT         4189 a         4862 c         5472 g         4382 t
ORIGIN      
        1 tgcggtggaa gcgggatttt tggaggtggt ggaggggtat gaacggctga cgaatgccat
       61 gctggccgat tgaaaggatg gcaggactat caatattcat cggattgtaa ccgattgtag
      121 gtcctttata tacataatct tacattcctc tgtttgccct gctccttcag acgcttatcg
      181 caccaggcta tagcccgctc cggacaagaa aactgaccgc cacagccaaa gccagtaacg
      241 ccatctgtgg caagatcctg ctgagaggca gatgctttcg ttctgttgct gtgccggtcg
      301 cgatgcccag agcagcacgg cccaagccgt aagcgacggc gctgaggagg atgaaataca
      361 ctatcagggc agggatgtag cctgctttga tgaatccaaa ggcgataaac agctcactga
      421 agaagagcgg ggagggaggc atgccaatga tgaacatgaa cgcgatcagc cacagccatc
      481 cactggcagg atgcaggccc agcagtcctc cacaggcgtt atagtccttc ttgccaaaca
      541 gaaaatagac gttcccagcc gtgagaaaga aggcggattt gatcagagaa tggcccatga
      601 tgtggatcaa tccggcctgg ccggccaggt ctcccaacgc gaaggcgatc aggatgatac
      661 ccatattttc gatggaggaa taggccagga ggcgtttgta gtttgtgatc ttcatgataa
      721 agaccgcggc gataaacatg gacgcaaagc ccaggatcaa aaagagctgg gaggccaggg
      781 tgcccaggtg actgatgttc atcacctgct gcatcctcaa caggggcaga agtgccgtgt
      841 ttaaaagtgc tcctgaaagt agtgccgaca cgggtgccgg ggcttctgaa tgggcgtccg
      901 gtagccagaa atgcatgggt gccagccctg ctttggtacc gaagccgatc agaatgaaga
      961 cgaaggagat tttgagccag aagctgttga gcgagcccgc gttggatatg atccgatcca
     1021 aaaaaaggct gggagaatgc ggctgggcaa tgatgagcaa caaaatgccc acaaaggcca
     1081 gagcgatccc gatggagcag ataaagagat atttccaggc ggcttcgatg gactgcttgt
     1141 gtttttcaaa gctgatcagc atggcgctgg caagggtggt ggtttccaca aatacccaga
     1201 tcagacccag gtcgcggctc aggctcgcgc cgtccatgga ggctacgaag agcatcatgc
     1261 agatggcgta gatgctgctc tggcgaggtg tcagctccgt cttcannnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnaggta taacatcctg gtcaatcctt cagggtggaa
     1441 agctcgtcgc tgtccacatc ttcaaagctg ttcctgattt tgttgataaa gagcacggcg
     1501 atcagaatgg aaagcaggat gtctaaagac acacccaggc tcacgatata gggcatttcg
     1561 ctgccgatgg ccaaagacag gagaaagatg ccgttttcca tgatcagata tcccatcagg
     1621 tgggtgatca gtttcttatt ggcgatgata ataaagaggc ctttgaggat ggcggaaaag
     1681 gcgatgccaa agtgaagggg atagatgttg gggctgcttt tcccagacca gagggcgatg
     1741 aaaaagccaa aggcaaagat ggccgtcatc gaacccagag aaaagaaatt ggacaccgag
     1801 ggctccactt cccgggtgat cccgtttttt ctgatggtgt cggtgataaa ccaggggatg
     1861 agcaccgctt tgaagacaat ggtttccaaa gccacaaaga taaactccag agtgttgagc
     1921 tcgttagtct tgaggatggt gagcgcgaag aggataaggc cctggaggat gaggatgcgc
     1981 gcgatgctgg acagcatgtt ggtgatggaa gccgccagca ggcttacgcc aaagataact
     2041 aagagaatat cagccactta gatgcctccc agattcctga cgatcaaagc cgccagggcc
     2101 agtaaggaca cggaaagggg gatgaccacc agctccagat tgcggttcat ccgatagcgc
     2161 ggcatcaggc tctccaccca tcccaccagg atggccagag aagtggtgac cagcagatag
     2221 atagctgtaa tggcaagcgg actgtgccct tccggaataa ggaagtagct gatcagagag
     2281 gcgaatatct gcatcttgag ggcagcaccg taatgcacca tcgccaggtt cactcccgaa
     2341 gtatccaaca ccataacctc atggatcata gtgagttcca gatgagtggc ggggtcgtcg
     2401 aagggcactc tggccccctc caccaggagc atgataaaca gggcgatgga tgtcatcacc
     2461 gcgatgatca cggaccagtg attggagagc accactgtgt gccgcagaaa gagatcaccc
     2521 atgctttcaa agcctccggc ataaaccatg gagccgatcg tgagaaagaa agcgggctcc
     2581 agaaaagcgg tgaaagatat ctcacgggaa gctcccatcc cctcaaaact gctgcccacc
     2641 tccatggccg ccagcaccat aatccctttg gataaggcca gcagatacat aaagatcaga
     2701 aagtcccccc ggaaactgaa gaccgcggaa ctggccccaa agggtaccag catggcagcc
     2761 atgagagttg ccgccaatgc cagcgacggg gccacgcgaa agatgaagcc agccgtgtgg
     2821 ctgtggacct cgctcttgcg cagcaatttg gccacattgt accagggttg caaaaggctg
     2881 gggccctttc tgcctgccca gatggctttg gttttggcga tcaggcccat gttccagaga
     2941 ggcaagatga gaataaacac aaggttgact atcacttgga tactcccagc acccagatga
     3001 gcaggatgat caaggctccc agaagataag tgatatagct gttgatcttg ccgttgtgaa
     3061 tcctgtcgaa gaggtccaga atccatctga gacctttggt cagccatgca aagatgccgg
     3121 aatcgagata gtcgtgcact tcctcttcaa agctgagttc ctgagggaaa tagccagtta
     3181 gcactgattt atcggagctt ttgtgcatga ggggtttgag gaaataggca agcggatcga
     3241 taaaagccgt gccggtatat tgcatcttgg catccggacg atggtatccg caaccccagg
     3301 ccggtccttt tgaaaccttc cccagtctgc gtttgagcag ataaaccacc gtggcaaagg
     3361 ttatgaagat gagcagaata acgctcagct ggtccagcgt gtggcccaga ctcaagatgg
     3421 cattgggatc gatacctgcc aggccagtga gaggatagac cagatacaat ccggccctgc
     3481 caaagatccc caatcccaga cagaggcagg ccagcaggat gggactgatc agcagaccag
     3541 tccttctttc actggcatga tcggcactac tggaacgggt cgagccgctg aaggccacgc
     3601 tataggcctt ggaaaatact atcaaggcca gagcactcac aaaggcaaat atggccccgg
     3661 ccacaattcc tgtgatggcg gtgccgatat cgctggcgtt gaaaccactg aggacgctca
     3721 ggaaaatggc gaattccgag ataaatccgt tcatgacggg catggcagag attgccatgg
     3781 tacccaggaa aaacagagca gctgtgcggg gcatgcgttt atccaggcct cccagagtgt
     3841 tgaggtccag gctgtgagtg gccagcagga tgttgccgct gagatagaag agcatgggtt
     3901 taaagaggga gtgattgatg aggtgcagca tcgctccgct gaatcccaaa gccgccatgg
     3961 tgctgtttga gcttataagg cccagttgcc agaaactgag gccgatcccg attatgccaa
     4021 tgttttcgat cgaggaccag gccagggagc gcttgaggtg ggattccgcc agggcgtgaa
     4081 taatcccgtt gaaggcggtg atcagtgaga cacctgtcag taccaggact tcaaccatgg
     4141 tccaggagga attggccagc acatacacga tgccaaagac acccgtcttg atcatcagtc
     4201 cagacatcat gccggaaaga tgagatgggg ctaccggatg agctttgggg agccaggagt
     4261 aaaagggaaa gaatcccgcc ttgaaggcga aacctatgag cagtagccat ttggccgccc
     4321 cctgaaactc tatcccgctg taattgaaga ttcccgattg ccggtagatc aacccaaagc
     4381 ccagcagcag aaaagccgcg ccgatatgca tcatgatcag atagtagatc gcgcnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccaga accacggatg ccaggccagc
     4561 caggacggta aacaatccag ccttacgcgc cagggcggag agtatggcaa tgatgaaaat
     4621 gatgtaaccg atgccgatca cacattcctc cccagcatgg aaatcagtcc atggatgatg
     4681 gagagaggat gagcagggca gccgggaatg tagagggcag gagtgatgct ctcaagccac
     4741 tcgcggctta aagctttgct accgttgaat atccctccgc tgatggcaca ggttcccgaa
     4801 aggatcagga ctttgggtgc aggtatcgct tcccaggttt cccgcagcgc catggccatg
     4861 tttgcggata ccgggcccgt aaggataagg gcgtccgcgt ggcgtggcga agccaccaca
     4921 tccagtccgt agcggcccat gtcaaaattg acgttggagc aggcccccag ttccagttcg
     4981 caggcgttgc agccagcagc cgagacgttt ctgagcgcca gggaattgcg gcataccttc
     5041 gccatcacgg gatggggact cacacccgga cggctggagg catttaactc ctcatcccat
     5101 ctgatgatca agccctcccg gctgctggag gcgagcctga aatccgcttc aaaactgaaa
     5161 gtctcgggaa aaccgcgttc acaggccccg caaaaaacgc actttcccag atcgaggtgg
     5221 tggttttgga tggcatgggt gggacagatt tgttccgccg cttcaaaatc ctcagcgtgg
     5281 tccccgagca gaaccggcac acctggaaaa gctggatgga tggaggcatg gaggggatct
     5341 gggatggctt gccagccgtg ccggatcctg gctttgatga gctcaaacat gccgcctcct
     5401 cacagatcgt tcccgcaata tgagagatca aagctcttgt tgcacagagg gaagtctgag
     5461 attccctctc ccctcacggc cagggccaga ccaaaccaat tgtgcagaga ggggtcgtaa
     5521 atcttgtaaa actcagtgtt ccagccggat gaaatccgcg cggcgtgcac gatcctgccg
     5581 cgccatcctt ccgtgacggc aatcgccaaa gcaccttgag gaggttccgg aagctcgatc
     5641 cgttggagtt ttagctcagg caaagtgctg agcaaatact ggatgattgc tatggactgc
     5701 agtgcttcct gataacgcag ccaggccctg gcatagacgt ctccgctttc ctgcaccaac
     5761 ggtgcaaaag gctctggata actgattaca tctccaaaga tgggaaagtc cagccttgca
     5821 tcccttgcca gcccagcggc tttggcagtt attccgctca tgttgagctc ctggacagcc
     5881 tgtctggaaa gagtaccggt gctgtcgaaa cggttcagca ctcccggatc ggcaaaggtg
     5941 gcacgcgcca ctctatctat ctgctggcta acagcttcga gggtttgcct cagcaccttg
     6001 ttctgaagct tgctgatgcc atagtttatc ccgccgggag caagccagcg cttgccatac
     6061 ctgcttccgc agatggccag agaggagttg atcaccgtgg tcctaagggc cgcgaacata
     6121 ttgaggccac tgagataggc aatatctccg cacagagcgg atagatcagc caggtgcatg
     6181 ccgatccgtt ccagctccag agcaatcatc cgcacagtgt ggacctcggg gcttaatctc
     6241 acaccagcca aagcttccac cagcaggcaa taagccagag catggcccac tgccgtgtcc
     6301 ccggcgatcg cttccgccag gcatgatttg tgacgaagat caccttctct aaagagcttt
     6361 tccacgcccc gatgctgata ccccagctgg atttccagat gctccacccg ctctccatga
     6421 cagataaaac gaaagtggcc tggctctata actcccgcgt gcacagggcc caccgcgact
     6481 tcatgcagtt ctttggacgt ggaccgaaag aaaggatagt ctctgtgatc gagtccctcc
     6541 atgccaggaa agcgcagcgg tttcaaccag ggatggccga tggggtggat gccatactct
     6601 tcccacagct cccgctcgaa catgtgaaag gcgggaatat cttttgccag agagggatag
     6661 ctcaaatcag ccggaaaatc cgtctgcgta acagccagct cagaacccgg cagtttcagg
     6721 acacacagca gtttcccgcc aggggttcca aaatagcata ggggatatcc tcccgccgcg
     6781 cagcttgatc tgatggcctg aaaaaaatcc gcattggaag caggagaagg aggcttaaga
     6841 cagagtggat tgcctgtaag tgactttgtt tccatgacat gttactcgct tcgctgcacc
     6901 gcttctgtca atcgaaaatc cagctgaagc tcatttgagg tgttttaagc aggcaggtca
     6961 gcgcgcgaaa tcaacccggg tgccaaagat atccacgcta aaagctcgcg ctgagcctat
     7021 atcgttgttg tagaaaagat agccctcttt catgccgccg ggcagcaggt ctccaaagct
     7081 gaagtgggag ttgagcacct cgaaataggc ttcgttatac ttctcgttgt tgcgctccag
     7141 attactggga gacagaggat cggtattgaa aggatcttcg aactcagcta tcaggtagcc
     7201 gctacggagg ctgcccagca cgtattccag cggcacaaag tcatactgcc ggcctcctga
     7261 aatgacgcca aaggaggagc ggttgaggtt cacagtcctt ttgctgaggt tgcgggcctg
     7321 gacccagagg ctgaagaagt tggagcttac attggcggcc gttccggaat acgcaagcgg
     7381 tctgaccgcg atcagcaaag agtcgttggc cacgatggca tagtgctttt ccacgccata
     7441 acctgatccg ttcagaggct gccagggacg catcacgcag gcgcttccga tcgatgcgat
     7501 caagatgagc ataagtctgg aagccaacga ggaaagcatc cggaaacgag gatgtttccg
     7561 gccccacttg gccccctccc ccatttctga gggaggggca aaggggatat ttatggaatt
     7621 attcgttttc atcctcaatc ggttcatcga tctcgtcttc agcatccaga tcgtcgattt
     7681 cttcttcctg atcatccagg tcttcgatct cgtcttcatc actggcggga atgacggtcg
     7741 gaatgggagg cgcagatttg atcttttcca cgctcttttc gatcacgttt tcatcttctt
     7801 cattgggaac gttggtgatg tcgcgcacct tgtcgttatc attgagcctg atgagtttaa
     7861 cgccctgggt attgcggctg atctcagaga tttcactcac attttgccgg atgatcatac
     7921 cttcctgggt gatgatcatc agttcttcgg actggtccac cagcatcagg gcggccagat
     7981 gaccgttgcg gatggtggtc ttgagggtga tcaccccctt ggagccgcga cgggtggtgg
     8041 gatacgctga gatggttgta cgtttgccaa agccgttttc gctcacggcc aaaagagtac
     8101 gggtaggggt ttcgccgttc tccagcatat cgtcctgact gatcacggtc atggaaatca
     8161 catagtcgtt ttcacgcagg cgaatccccc gcacgccttt ggtgaagcgg gcggtggcgc
     8221 ggaagtcctt ttcattgaaa cggttgcaat agccattgcg ggtagccagg atgatgtcgt
     8281 cggcgccttc agagatgcgg gcatcgatca gctcgtcgcc ttccaccagc ttgatggcca
     8341 gaataccagc gctgcgggga cgtgagaacg cggtgagcgc gctcttcttg atcagaccgt
     8401 tgcgggtgca catcacgatg ttgtgctgag gatggaagcc cctcaccgtc acaaaggctt
     8461 tgatcttttc atccggctgg aaattgacca ggttgacgat tgctttgccg cgcgcggtgc
     8521 gtcccgcttc cggaatctcg tacactttga gccaatagca gcgtccgtgg tccgtgaaga
     8581 gcaatatata ggaatgggtg ctggccacaa agacatactg gataaagtcg tcttccttga
     8641 ggttggcggc gttgaggccg cggccacctc tcccctgcac tctgtaagta tctactggca
     8701 ggcgctttac gtatccggca tgagtgatgg tgaccaccat cagttcgtcg gcaatcagat
     8761 cttcgatgtt gaggctgcca ccgtagtttt cgaggatagt ggtacgacgg ggatcgccaa
     8821 acttatctct gatctccctg gtttcctgtt tgatcagttc catcctgagc tcgcgtctgg
     8881 ccaccaattc tctcagacgg gcgatggtga tcagcagttc ctgatactcc gcctcgatct
     8941 tatctctctc gagccccgtg aggcgttgta aacgcatatc cagaatagct ttggcctgga
     9001 tctcggagag cccaaacttg agctgaagct gttcattggc ctcgggagga gtctgggaag
     9061 cgcgaatcgt ggcaatcact tcatcgaggt tttccagagc gatgcgcaga ccttccagaa
     9121 tgtgcatgcg gtcttcggca ttcttgagtt caaagagggt gcggcgcaac agcacgtcgt
     9181 ggcggaattc gataaagttg gtgagcatgt ctttcatgtt caccacctgc ggcacgccat
     9241 cgatcaggca gaggttgatc acgccaaagg tgctctgcaa ctgggtgtat ttatatagaa
     9301 ggttacgcac cacctgacca tcgtgattgc gcttcacctg aatcacgagg cgcatgccgt
     9361 cgcggcccga ttcgtcacgg atatcactga tgccttcgat ctttttctct ttcaccagat
     9421 ccacaatgcg ttcgatcaac agagtcttgg tgacttgata cggaatagag cggataatga
     9481 tgaattcctg ttcgttgtcg ttggtctcca gatcggcctg accgcgattg aggatgcgac
     9541 cacggcccgt ttcaaaatag tctctgatcc catcggcacc caggataaaa cctcccgtgg
     9601 ggaaatccgg acctttgatg tattgacgca ggtccagaga ctccagctcg ggattgtcga
     9661 tcagggcgat catggcatcg caaacctcgc cgatgttgtg gggcgggata ttggtggcca
     9721 tgcccaccgc gataccggat gagccattga tgagcagatt ggggatgcgg gccgggaaga
     9781 cggtaggctc cttgcgggtt tcgtcgtagt tggacttgta atccaccgtg tccttttcca
     9841 gttcgtccag catttcgatg gtcactttat gcagccgggc ttccgtgtaa cgcatcgccg
     9901 cgggcgggtc gccatcgatg gagccaaagt tgccctggcc gtcgatgagg ggatagcgca
     9961 taatccatgg ctgggccagg cgtacgaggg tgggatagac cacctgctcg ccgtgagggt
    10021 gatagtttcc gctggtgtcg cccgcgatct tggcgcattt gcggaaatgg ccaccgggac
    10081 tgagattgag ctcgtgcatg gcgtagatga tgcgccgctg agaaggcttc aggccgtcac
    10141 ggatgtcggg cagggcgcga ctcacgatca cgctcattga gtaatcgaga taggcctgtt
    10201 tgagttgttc ttcaatctgg atgtttatta tctttgtctt gtcttccatt tatatctcca
    10261 tggttcctta agcgttacgc gttaagcgtt aggcgtggcg cgctgcgcta tagatatctt
    10321 taagttgagg gtcactcgtc ttatcctgat atatctgttc tagcatgttc gtctgagttg
    10381 gtatcacgct gtatcccagc tatccatggc tgcctcgaaa cgcttcacgc gaaacgcgta
    10441 acccttagat atccaggttt ttgacataac gcgcattcgc ctggataaaa tctctgcgcg
    10501 gttccacttc atcacccatg aggatggtga acatgcggtc ggcttcgatg gcgtcgtcca
    10561 tcttgataga catgaggctg cgaacgctgg ggtccatcgt ggtttcccaa agctgttcgg
    10621 gattcatttc acccagacct ttatagcgct ggatcaccac acccttgtct ccaaattcct
    10681 ggaccgcctg atcccgttca gcttccgtga acacgtactt tttctgtttg cctttgcgca
    10741 ctaaaaataa gggaggcatg gcgatataga catggccatg ctcgataagg ggccgcatgt
    10801 atctgtagaa gaaggtcatc agcagggtac agatatgagc tccgtccacg tccgcgtccg
    10861 ccatgatgat gatcttgtga tagcggattt ttgtgacgtc aaaatcctgt ccgatcccgg
    10921 ctccaatggc caggatgatc ggctggatct tatcgttgtt caatacctta tccactctgg
    10981 ccttttctgt gttgagcatc ttgccccaga ggggaagaat ggcctgaatg ctacgttcgc
    11041 ggccctgttt ggcagatccg cctgccgaat caccctccac cagaaacagc tctgtcttgg
    11101 tgggatcgct gatgctgcag tctgccagtt tgcctggcag ggagccgctt tccagcactg
    11161 atttacggcg cgtgagctca cgggctttgc gggccgcttc tctggaacgg gctgccagaa
    11221 tgctcttcat ggtgaccgtc ttggcttcgg aaggatgctc ttcaaagtag gtggtgagtt
    11281 tatccagcac catggagctc acaaagcctt ccacatcact gttttgcagc ttggtcttgg
    11341 tctggccttc aaactgggga tcgctgattt tgacgcttac aacggctgtg aggccttccc
    11401 ggatgtcctc acccgtggga ctggtctttt cattcttgag caggtcggcg ctcttgatat
    11461 agttgttgac ggcccgtgtg agccctgtct tgaagccact cagatgagtg ccgccctcga
    11521 tggtgttgat gttgttggca aagctgaaga tgttttcctg atagccttcg ttatactgca
    11581 gcgacacttc cacatccatg ccttccttgc tgccttccat caggatcggc ttgacgccca
    11641 gcggcttttt attctggttg agatactcca caaagctcac gatcccgcca tcatacttga
    11701 agtcatgcag gcggtcgtta cgctcgtctt tgagcatgat gcgcactccc cgattgagga
    11761 aagccagctc gcgcagacgg gtggtgagat agtcgaagct gaattccacc gtctcaaaga
    11821 tggtgtcgtc tggcttgaaa cggatcaccg tgccagtgcg gttggtgtct cccagcacct
    11881 gcagatcggt gacgggcttg gccctttcat agcgctggta atggattttg ccatttttgt
    11941 ggatgcgtac ttccaaaaat tccgacagcg ccaccaccac cgaaacgccc acgccgtgca
    12001 ggcctccaga gactttgtag ctcttgtcgt cgaattttcc gccggcatgc aacaccgtca
    12061 taaccacctg caccgcaggg attttcatct cgggatgcat gtccactggg atgccgcgac
    12121 cattgtcgtc aacctcgacc accccttccg gagttatggt gaccttgatg agatcacaat
    12181 atcccgccag agcttcatcg atggcgttat cgaccacttc atagacaagg tgatgcaggc
    12241 cgcgttcgct ggtgccgcca atatacatgg caggacgctt gcgcaccgct tccaggcctt
    12301 tcaacacctt gatgttgttg gcattgtagt tttgatcgat cataatctat ttactcctaa
    12361 taatttaagc aatcaaccag agtggcattc gtgagtccgt ccgccgcgtc cagcgttttg
    12421 atcgttatct gtatgcaaga aaatcagata tgggtttttt gtcaagccct aatgtttctt
    12481 tttttacgct tgtaatggcc tgtatagcag atagttacaa gttatgaaaa agcgatgctc
    12541 cgaaagcctg atttcccacc gtcaggatac atggtttccg agggcttcca gcacctcctg
    12601 acaagccttc aggcctcgcc cttgccacta tacccacgac agctccaaac caagcccgga
    12661 ccaagcccca tttgtccgaa gtgggtcggg tctgctatct ttctcattcc acccagagac
    12721 tcaggaagaa agcagccggt agaaccagag agacggttcg atattgcgga actcaagctc
    12781 tgaaacaagc gttgcaacgg cgttcacgcc agtttgtccc gcactccaaa gcaaaaaagg
    12841 gcccaccgcg agggtgagcc catatttgat ggttgtatat ctgtttattc ttccacgacc
    12901 ttcacttcca cgcgacggtt gagggcgcgc tgggctttgg tcttgttttt gttgagaggt
    12961 ttgctgagtc caaagccttt gtagctgaga cgttcctttt caacgccctt cttgatcagg
    13021 tagtccacca cggctttggc gcgacgctgg gaaaggccct gattgtattt ggcggtcccg
    13081 atgttgcagg tgtggccgga gatttctatc ttgatctcgg ggttgttgag catcgtgatc
    13141 acgatgtcat ccaggatcgg gaaagattcc ttgcggaggg tggctttgtc gaaatcgaac
    13201 tggatattat tgaagacaaa caccttcttg accaccagcc gctcgatcgc gaaatcgata
    13261 ttgacggcat cctggccttc cggaggagct ggaatgtcct gttgatagag catgaagcct
    13321 tccggattga tgaccaccgt ccagggatcg gcataaggca ctgtgatctc aaaagtgccg
    13381 tcctcgttgg tggtgaccac atccttgcgg gtttcgtcgt ccaccagacc ggtgaaggtg
    13441 atatccgtgg ccacaggttc gcctttttca tcggtaacct tgccggtgaa agtgatcata
    13501 cgcggcggct cttcgaccac gggttcttcg ataatcggtt cacccaaaga atcggctggc
    13561 ggcacatagg gtgggatgtt gacttcgtag acccgcgtcc cggtggtatc ctgggtcaca
    13621 aaatatcccg gaaccgtgta gtccacaaag gccagcagga ctttttcaaa gcctggggcc
    13681 tggcggtctg aggagatata tacctcgttg ctgccgggcc gggtggaaaa atagcggtcg
    13741 tcctggatgg aattgatcgg cagtcccagg ttttgggcca gatgccaatc ctgccaggtg
    13801 ggacctttgt ggacggcctt gaagaggtca aaacctccaa aaccgggatg ggtgttggaa
    13861 gagaaataga gggctgtttc tttgtactcg acattacggc cctcatgcac gatatctttg
    13921 gtgaaatgga ccagatgggg ggtctgttcg atgccggggg tgttgatgtt ggggccggcg
    13981 ttgaccggtt cggtccagac gccatccact ttctgagaca cccagatatc tatctcgcca
    14041 tatccgcccg ggcgtgagga gctaaagaac atcagcgaat cattatacac gctgggatga
    14101 gtatccacat caggggaatt gaaccacggt tgggggcttg gtttggtcca gcggctgcca
    14161 tcccattcgg tgtagtagat atcgccgtcc agcttgcctt tttcatagtt gccaaagaca
    14221 taagctccca cgctgtccgc ggcgaatgat cccatggctt cattgctgtc cgtggaaagg
    14281 tccgtcacca gctggggttt tccgtagccg gatttgaaga tctcggcccg ccagatattt
    14341 tccttgtcag ctttgccggg acgccaggaa gtgaaataga gatactttcc ggccggatcg
    14401 acaaccgctc cgtagtctga attgcggctg ttgatatagg ccggcatcgc tctcagactg
    14461 atcttttcaa aggctttgtc ctgattgccg atgatctcga tgaaggtatc cacctgctgg
    14521 cggaactcct gatcttcgct tttcacataa cggtccagcc agaccatggc attggtggta
    14581 tcctgcacgg caatgtggag ccggcccagc caatagaaat agtagggcac gtctccattg
    14641 gcggccagat cggcctcttt gaaccgttcg atcgccttgt tatactcgcc gcggtggaaa
    14701 tactccagtc cctcggtggc ataacgttct cccttcatcc cgatctggat ggcaaatata
    14761 cttaccaggc cgatcaggag caggatgaca agatatgctt tcttcatcgt gttgcccctc
    14821 atcagaacct gaagatcatc gagatgatgt ggtcatcgct catgatctcg tggttgttga
    14881 ggcggaacac gtaatccagc tggaagttgc gaaatctgat cccggtcccg gctgagaaat
    14941 gtccctggtt gaatccgagg cgcaagcccg tctgagcata ggaaaataca tcttcccagg
    15001 cactgcgttc cttgacggag atcacggaga gcttggaatc ggcttccggg tccttgccaa
    15061 tcgaggtcca atattccgct cccacagcca gataggggtt ttcacgctgt tcccatttga
    15121 ggtccgcggc cagagtgacg ccgattaaag gataggcagc tacccccatg gccatttcga
    15181 tggggacgcg atctcccgcg taaccgccca gtaggtctct cagcatgagg ccggccacca
    15241 ggtactgatt gacgtcatat ctggctccca agtcgatccc cattccggct ttggtttcgc
    15301 catccagagt gctgaaatag aacttgggcg tcacgccata gtccaggtct ttccaatgat
    15361 tggcatagct gacggagatg ttgtgctgat tgtcgctaaa ggtgccggtg gggtttccgg
    15421 cattgtcgta accctcgatg tctcccactc ccgcattaat ccaattgaag gccagggtgc
    15481 caaaatcaaa gggtttggcg atggaagcgt attgatggct gcggtccagg ttcatgctga
    15541 agttgtacat gacccccact tcgatgtctt tgatcttgtt gaggccggcg gggttccaat
    15601 atccggccgc gacgtcacgg ctgatggcgc ttccggcttc acccatggca atttcgcggg
    15661 ccccgatgcc gaggcgcata taagctccgg cggagttgtt gaggggacca gccatcagcg
    15721 gcgtgagcag gatgccgagc agggcagcga ggagcaggac tttggtgttt ctatgcttgc
    15781 gtttcatttt atgcctcctt atttgaccga gatcttaatg gtgcgttcgg ctttcttgac
    15841 cccgtcgttg gcaacgatgc gggcaaagta ggtgccgcgt cccactttct tgtggctgga
    15901 attgttgagg tcccagggga tttcgatcag attggaattg gtgggcggaa cggtgagggt
    15961 gcgtaccttc ttaccgctga agtcatagat actcaccgcc acctggacgt tatcgttgcg
    16021 ggtgagcacc agcttgattt tggtggatga gccggcggga ttgggatacg cgtaggtggc
    16081 ggggctgata tccagctcgc cggattggta ttcgatcgtg gtggtttcgc tggggttgct
    16141 ttccactcca ttgtaaatgg caaccacgcg atacacgtat gaagtgccgg cctggatggc
    16201 ataatcgtcg tagtagtaat ccgtgccgag catcgtgagt actggagtgg tgttttgttt
    16261 ggtccaggcg gcttcgctgg tcttgcgtct gtagatgtca aaaccatcca gagctttacc
    16321 gctttcaaag ttccagagca gacggatata gcctccatag gtgaaggcgc gcacgtcata
    16381 gggcaacggg atcaccgtga aggaatggat cacatccgaa gatggaatcc aatctgtctt
    16441 gaaggccttg gccctgatcc ttacgtcaaa gctgagctct ggaatcacga tcggctgggc
    16501 cggatcaaac agagtggcgg tctcgtccgg aatggccgga tcgcttccca ccgctacggt
    16561 ataatagatc atcgcgcctt cagtggcact cctgagctcg attgtgaggg gatcatggaa
    16621 gagctccgcg gtgatgggct ctgaattttg atagaagtca ggagcttcca cattgcctgt
    16681 cacgacatag cggaccgcta tcacttcact gtcaatccag tcgcgggcaa agccgcgaat
    16741 cttaatcacc cagttgctgt taagcggaac ggtgatgggg ccggtgtatt gcgtggcagt
    16801 ggaatcggga tcgagagcgt cggcgggatc tgccaaatcc ggagtgagcg tgtagcgtag
    16861 aatggctccc tgaactggca ggacaggcgc tgcaatcgtg accgtctgag cgctggtgta
    16921 ggtccctggt gctggactga agggactggc cgccagctgc acctgaccgg tgaccgcata
    16981 ggtggcttca tacacagggc tggggtccca actggccaga taggccctgg ctttgatcgt
    17041 catcagtgtg tttagaggca cttcgatggg agcgctgtaa actgctgaag cctgggtggg
    17101 caccgaaccg tccgtggtgt aatgaatcgt agcgctggac ggaacgggag caccgatcgt
    17161 gaccagggta gccgtctggt agacgactcc gggattggga ctgaagacca gcggcgggat
    17221 gatgacctgt ccggtcatgt tatagctggc actgtgcacg atgctgggat cccagttcgc
    17281 gtctccataa gccctcacct taacggtgat ctgctgatcg agattcagag ggatggcatc
    17341 ggtgtagaga gtgccgttgg tagtggtggg atcgctgccg tccgtggtgt aatagatgct
    17401 ggcgttggag ggcagagtgt tgttgctgat caccaggctc tgagcggtgg tgtaggtccc
    17461 ggccggaggt gtgaagacag ggccgttgat gatcgcggcg ccattgatca catatacacc
    17521 ctcataggtg ggactggcct gccaattggt ggcaaaggcc tttaccttga tccgggtggt
    17581 ggcataagcc ggggctggaa tcgccgcgct gtagatcggt gatgtagctg taggttcgct
    17641 gccgtccagg gtgtatctca ctacggcccc ggcgggaacg gtggtattgg agacggagat
    17701 ggactgcgga ttgatgaagg tgcccggggg cggtgtgaac acaggagctg taatgctcac
    17761 ctgaccggta acggtgtagg tagccgtata ggtctggctg gccgtccaat ccgtcttgaa
    17821 ggccttggtc ttgattgtgg tgctggtgtt taaggccagg ttgatcggag tggtgggata
    17881 ggtgaagccg ttggtgaggc tgggctcgct gccgtccaga gtgtagcgtc tgatcgcgtc
    17941 cgtgggagtg acgttgttga tggtcacatt caccgggctc gtatagatca ccgttgggtc
    18001 cggaaggaac actggcgtca tataggcaac ggttccggtg atggtgtagg tggccgaagc
    18061 gatggcgctg ggtacccagc cagtcttgta ggccctcacc ttgatagtgg tggggccgct
    18121 gttctcgggc agagcgatgg gaccctgata gagcagggaa gcttcggtgg ggtctattcc
    18181 gttcagagtg taatacaggc tcgcgccact gggaatggca ggaggattga tggtgatatt
    18241 ctgtgcggtc tgataggtgc ccgctacggg tgtgaaagta gccgcgggta acacgatagt
    18301 gccagtgacg ttgtacacgg cttcatagac cgggctgggg tcccatcccg ccaggaaagc
    18361 cctggctttc actgtcttca ccgtgttgag cggcacgctg atcgtctgcc cggtgtagat
    18421 tggtgaagtg gcagtgggat cagtgccgtt ctctgtgtaa cggattgtgg ccccggccgg
    18481 aagagtcgtc gtcgagatgg tcacaggctg cgcggtggta taggtgccag cgaccggagt
    18541 gaagacctgg ccgggaatcg tgacctgacc ggtgataaca tatgtctggc tgccgatcgc
    18601 gctgggagtc cagccgttca cgtaagcccg ggccttgatt gtggttgtgc tgttcaaacc
    18661 aagttgaaca gcctgagtgt attggctgga gctttcagtg ggatcgctgc catccagagt
    18721 atagaagatc gccgcgccag gagtgggagt ggtgatcacc acgttttggg cggtggtata
    18781 gctgccaccc agaggattaa agaccacatc ctgcacggtc ccggtgacgg tgtaggtccc
    18841 caccgcgatc tggctgggat cccattcatc cttgtaggcg atggctttga tcgtcatcac
    18901 ggtatgattg ggtacgttga tgggaccaag gtatggatcg gaagcggggg tgggatcgct
    18961 gccgtcggtg gtgtagcgga ttgtggcttc cggagtatcg gtgcggatgc tcacactgac
    19021 cggcgcggtg aaactgccgg gcaggggata gaacacaggc gtcgccacca ccggcacgta
    19081 tgaacctata gcgtaggtgg cgctc
//
LOCUS       DAOSFN010000032        18966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2355_length_18966_cov_9.199408, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000032 DAOSFN010000000
VERSION     DAOSFN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 18966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18966
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_18966_cov_9.199408"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLO35_03515"
     CDS             <1..512
                     /locus_tag="PLO35_03515"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011073447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOH78764.1"
                     /translation="ALIAAMDRNRVIGKDGKMPWHLPEDLHWFKTNTLGHPVLMGRRT
                     FESLPGPLPGRLNLVLSRDASYTVPPDVVKCSSLEDAFQALQQGAAESQTAGLSHPPL
                     FVIGGATLFSQFLPQAAFLYLSLIDADYEGDTWFPEYNSLGWNTLYEERIVSASGIPI
                     SFLILERNQ"
     gene            complement(677..2314)
                     /locus_tag="PLO35_03520"
     CDS             complement(677..2314)
                     /locus_tag="PLO35_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOH78765.1"
                     /translation="MATFKPSQIMAESFAELIIPGVDIVRLNASHFNQADLEEVLHKL
                     SKLPVKVMLDLPGPEYRIYGYAPEHELQDGQTLEIGNNTRAYHTDFYDWHLLRPQMRV
                     LIQDGQIEAGIISLKPDGAELCVTRGGKPRASAHLSFPDLPSEANSLTDKDKSLIRFA
                     LEHQIAQIALSHINHPAYIIQAREFISALAKDHKPALVVKIETKTALANLDEIISQSD
                     ILLLGRGDLGSQVPLWEIPLAQRRLIRQAQRHGKAVYIATQMLPSLFYDRVPSRSDVS
                     DIAFAVLDGAAGITLTDETVRHDDPRLAISWGRAIIEGVLQMHTAWDASAPLPDQELI
                     SKLSDIGTRIWARGWAEANAGNVSIRLTDYGDDPLAPITYLVSRTGSRYRQIADDPMQ
                     SLCLIEAQAEQWHSLSPEAKPTSEWSAHLKLHQLFRAQGNDIRVVLHAHPASVIAISH
                     LPIFQDETLFNLELAEILPELPLYLAKGVCCCPTYPPGSIELAEASASCLGERNAMIW
                     QKHGLLCFGKTLDEAFDYMEIVVKAAELWLTCHHRKD"
     gene            complement(2342..3871)
                     /locus_tag="PLO35_03525"
     CDS             complement(2342..3871)
                     /locus_tag="PLO35_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH78766.1"
                     /translation="MLQLHQRFIQTAKQHGNRIAVYDKATSKDYTYSRMLIASLILKE
                     HIGKIKDKYVGIMLPTTMGCMLGVIGTLMNGKIPVMINYSTGALDNCRYAREKCNFKT
                     ILTSRKLLEKLDLKAIDHMVFVEDILASVTAVAKLKAALISKLPFSILKGMVHSGPAS
                     EVSVILFTSGSEKEPKAVQLSHRNILHNVDAVPQMVRLDHTDTFICMLPLFHVFGLTT
                     NFWLPTILGATMVTYPNPLEYKTISDLVREYKVTFMTATPSFFYGYLQKSQPGDYASI
                     RFAIAGADKLPMKVYEGFLHKHNITVFEGYGATETSPVISSNYPGVHKTGSIGKPIPG
                     VHVRIVDIHTDKVLGPNQEGKIMVKGDLVMEGYLGDLEETSLRIRNGWYDTGDIGIID
                     EDGYLWHRGRLKRFVKVGGEMVSLVKVEDVLSRLLPEEVICCVVDVPNPTKGADVVAA
                     VANGDFDMKSVLAKLKKELPSIAIPRQFYVIEDIPMMASGKVNFREVERICRERNSNG
                     D"
     gene            complement(4004..4642)
                     /locus_tag="PLO35_03530"
     CDS             complement(4004..4642)
                     /locus_tag="PLO35_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78767.1"
                     /translation="MPEIMQVIGIWLAAFFTFCIFSFLYKDNPFYKLAEQIFVGLSAG
                     YGLIYVIYTTLLPNLFTKLFSDFGGNLILLIPFALGLMMLTRIVPKAQWISRYPLAIV
                     IGTSAAISLLRYLKSDLINQVGATMINPFANASIPEVIGNLMVIIGTLCGVYFFYFSK
                     KQEGLAKIPSKIGVYFLMISFGATFGYTVMARVSLLIGRLEFLLKDWLHIIN"
     gene            complement(4635..5492)
                     /locus_tag="PLO35_03535"
     CDS             complement(4635..5492)
                     /locus_tag="PLO35_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78768.1"
                     /translation="MSVNTSSHTERRVIFGIVFLAVMIPLIWPIGWTSEVSPIVRAAH
                     QLVDDTAASSYGLISFDYEASTITELHPMAVAQIEHAWSKGQKVIATALWPQGPQMAA
                     QCFEEALKNPVFKDKQYGVDYVNLGYKVGGLVTIQAMGSDIKSVYPKDSSGTDWDKLP
                     MLKDIRNLKDLAWISSLSSGVPGLREWIMIAHDVHKIPVTGGTTAVSAPGFLPYVNDQ
                     HQLTGLLGGLKAASEYEMLIDKKGPATTKMDAQSVAHIVILLFIALGNIKAWRRRNPA
                     APKPGGNNA"
     gene            complement(5489..6187)
                     /locus_tag="PLO35_03540"
     CDS             complement(5489..6187)
                     /locus_tag="PLO35_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78769.1"
                     /translation="MKRNLPLILAFIAGLVVLFSEFIPHRPFSAIVDTLQEWFMIISG
                     FAMLLGLISLLSMNASRISSKSQDWQYYLAGMISFALMVLMGALWGMEERPGMLGQGS
                     LVSSWLGKKPFDYLYDNAFVPLSATIFSLLAFFIASAAYRAFIIRTLESNLLMIAAVI
                     VILGRTGFGTMLTGWLPDSLKFWHLPNLADYIMQYPNSAAQRAIMISASLGIIGSGLR
                     IILGIERSYLGGDK"
     gene            complement(6184..7350)
                     /locus_tag="PLO35_03545"
     CDS             complement(6184..7350)
                     /locus_tag="PLO35_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78770.1"
                     /translation="MTRFAIIVLLLALHSLALGAQAAPDAIVPASRLVPPDISEASLA
                     VIDKPWDKGESLIIDWKLPAGIDSLRLQKADSLGLFVDLEASYSPEGSYTDGDLNPDK
                     QYRYRMIAYQKASGDSIILSALAQPKENWFYMDRLAYLLLFLIIGSAIVYYILAARRG
                     KDFFIRRIGGLDSMEEAIGRATEKGKPILFVPGISELDDIQTIASLSILGQLATRAAE
                     YDTELIVPCRFAMVLSAAREVVKEAYLKAGRADSYKEDRVFYLTDDQFGYVAGIDGII
                     VREQPAANFFLGSFYAESLILAETGLSTGAIQTAGTANAHQLPFFVVSCDYTLIGEEL
                     FAASAYLSRDPQQVGSLKGHDFGKLLVIILILIGVALEIAGLGWFKTFLMGAGS"
     gene            complement(7462..8265)
                     /locus_tag="PLO35_03550"
     CDS             complement(7462..8265)
                     /locus_tag="PLO35_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78771.1"
                     /translation="MKRTLLILMAILLAMTIVSCKGNKEAAKLPDDEIKAQVATYAPL
                     PSFKEVFRTLDQVPAKDFSAVIPQKLFKTKQEASRNAFALGVLTADAVMAAKARNKAK
                     LTEISQEMMNLTTLLNLEDEINRMGANLSTLIAQEKWEDLDASLDQVKKSVEDKLWEL
                     ENYEHYTLMILGGWTEALSNVSRLLANNYKAENTSAVNQKGTWNSLSANFGLVSTPAI
                     KDSAPYQAAVPLVQKVKDILNADTTKTYSKEQLQQLIEATDQIKAAWQQ"
     gene            complement(8277..11546)
                     /locus_tag="PLO35_03555"
     CDS             complement(8277..11546)
                     /locus_tag="PLO35_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH78772.1"
                     /translation="MPKSDKNLKLGDRQLKKTIDMITRLYVHVIKSEDQISASEIDIL
                     YSLLINLFRDVNISWEVYVRNIMETEDDFGQISQYLNQHLNVLDKIRIILSLIILANT
                     DNSIEISELTGIIALCRKFELSPEPFVELMDHFESGIQECVAIPHDIQYQHLQDSLFT
                     DYVSIGSSSLADMRFKDPGIGELDCILFGIDKSLFLSTSFSCTSKLNGLDLKPSCVIL
                     LPPEGTLEVRGLAYDIESLWKIYRVAEDDDDIEFKKPDYDFLISRHRHQYSILVNGGT
                     ITVNDKELVHGRKLELFYDDVLQIQGYAKFTVRDVIRERSSIGIDTMVPRELFLIAER
                     DFFYLSRVETERAIARIEVEKGKFYLHPPKRGWTIFHNQQRVKELTQFKLNTDILTIA
                     RRNFRINGFYDLVETPFEVQSLTIQDVKHYFQDGKLALDGISCDIRKGELIGILGQSG
                     CGKSTLVKTITSELVPTYGQVKIDGKVLYNNLSYYSQFFGYVPQDDLLYPNLTVYENL
                     WYRGRLRLPNISAASLKQKIENLLHQVNLSHRKDTQVGEPRRTMLSGGERKRLNVALE
                     LLFEPTVIVCDEPTSGLSWGDAEQVIDILKLLTAQGKIVILTIHQPNSSVFRKFNQVL
                     LMDLGGRQAFFGPPDECFDYFDYELAQLGYRREDIERKKQLHNSDYMYEVITYPQYDD
                     SNAPVYEQVNKLLQVKRKFPPEYWRDKYKRKMLFEIIQLEPADPPARTSIPKRRHRKL
                     DFRSRLVHLLAFITRSLTMKLRNRTNNLITFVEAPLLGLVISFILRHTPRSGPYNYND
                     NNNIGIFIFVSIIAFMFMGMSNSVEEILSERKIILREKLMNLKVSFYLASKLIALTFF
                     SLVQVLLYVLVASLVLEIKGMWGISILYLLLTSIVGFSLGLTVSSFLKDNKSIINLLP
                     LLLIPQIILGGAVIEYERMNRRLTLLERHPVPEVVQLIPSRWIFEGLTTAYAKNTVFH
                     RKLAEIEKKRLTYNAMFKDNRLDAAQYRKLIAGISNQRDYVADKWDPTKVTNANLNAS
                     VGLMDGQFHVDRRNVFLSSYKLIKGKIHRTWNYNVLMILLYVLGFNLITILKLKYYFK
                     E"
     gene            complement(11550..12845)
                     /locus_tag="PLO35_03560"
     CDS             complement(11550..12845)
                     /locus_tag="PLO35_03560"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOH78773.1"
                     /translation="MDERFLHHIWDGGHLKMELATVSGKHLRIGYSGQYNTNRGPDFL
                     NSGLVIDGLAVRGDVEIHQLTTDWHAHNHAEDHFYNKVALHVVFRHNSSRDYTIKENG
                     EAIEILELKEQLSEDIDKLLREHQPRLPKGRPAYCDLLSALDNDRLGLILRDIGLKRF
                     LGKVNRFNASLMFSGFDQILYEGLFEAMGYDKNKTAMLLLAQDIPLARMLEWSAEGLS
                     MIDLLAIYAIASGLLAKSQKLVPDQLTEILQQAWQAQNRFGERLSIDWQLFRIRPANH
                     PIYRLLALIPMVHRYAQDGLLSSLLRELHPRGTQKQWDKGFTELFSSTLLPGAEMLPR
                     PGQGVLSSIYVNICLPVFYLWGQKTSDNLLMTETRDAYLKHRGLAGNQLIRMMSDHTD
                     PRQQKLINSKAVYQQALIELHQRFCRYHICDECRTQRIG"
     gene            complement(12845..13546)
                     /locus_tag="PLO35_03565"
     CDS             complement(12845..13546)
                     /locus_tag="PLO35_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOH78774.1"
                     /translation="MGKLSSSSQTGKKRRPWLIRFIRLLWFCHLAFWGVVAFFCLLYI
                     FVNPPITPLMLQRYMIRGFDWHKREYIDLEKIPDSTVDMTIVVEDGNFYRHYGFAIEE
                     AKRAWKRNKTSGKIRFGASTISNQVARTIFLTTDRNYLRKYLEAQVTVIMEVLMTKNR
                     MLELYLNYVEWGKGIYGIETASLYYYGRSCSRISRDQSMKLVSILSSPIKYSPQTYSR
                     SASARQRYRMLNRYF"
     gene            complement(13604..13867)
                     /gene="rpsT"
                     /locus_tag="PLO35_03570"
     CDS             complement(13604..13867)
                     /gene="rpsT"
                     /locus_tag="PLO35_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOH78775.1"
                     /translation="MPQHAAPRKRMKTDKKRQARNNYVKRTLKTLSKQMQSELPAPEK
                     TALLDKIYSQLDKAAKKGVIHKRTASRRKSRVAALVNKLNKSE"
     gene            complement(14050..15348)
                     /locus_tag="PLO35_03575"
     CDS             complement(14050..15348)
                     /locus_tag="PLO35_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOH78776.1"
                     /translation="MNQRRLLLITYYFPPCGGAAVQRWLRLLPYLLKAGWEVHVLTTS
                     GGDYPQLDESLLASLPSELNIHRTPAPKLGRLWKLLLGKGSELPHGDLRSSPQDPLLR
                     RVLIWVRLNLIIPDLRIFWNPSAYRDAKAIIQERGIRTLITTGPPHSTHLLGLPLRKS
                     FKLRWIADWRDPWSTIHYLNLNPPSQFSLSAHKYMEGLVCAKADLNLVISRYLLNTLP
                     EGKKLLLMNGFDPQEIKRAKEEAGTIPTAGITAPGPQDFLISYVGQITAGQDLETMLA
                     ILADFRELPSIRVRFIGSRLSPEQQRSIDDRLGDVSETIGYLPHGTALVEMARSNLLL
                     MMINRYEGFEGMLTTKLFEYLGVGVPILAIGPRGGEAEILISTYQAGLCVDRDEVLAA
                     KAWIADLLRRHSLGERVIYDRDISALSSESQSRILLDALH"
     gene            complement(15348..>15749)
                     /locus_tag="PLO35_03580"
     CDS             complement(15348..>15749)
                     /locus_tag="PLO35_03580"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOH78777.1"
                     /translation="SVAGFFQRASLCVLPYRSASQSGVIALSYAFDLPVLASRLEGLE
                     QYVDEGRTGLLVNAVPEPRPEDLARELIRYFKGSLYETMTPYVSKKGAEFSWSRLGKI
                     IIGPGVNSSGKLASADSSADFIKSLHNSSES"
     assembly_gap    15750..15849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15850..16289)
                     /locus_tag="PLO35_03585"
     CDS             complement(<15850..16289)
                     /locus_tag="PLO35_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOH78778.1"
                     /translation="MKIAFLGPAPPLRGGISQFALSLASTWLAEGHQVKMFGFRRQYP
                     ALIFPGKSQTAEFILPSKLEIDNCYIPYRPDTWPQAVKKIRDYQPNILIVSYFIPFFS
                     PSYIMICRALKGVRITALLHNLVPHEPWLGARFLAQVFLERCDN"
     gene            complement(16488..17465)
                     /locus_tag="PLO35_03590"
     CDS             complement(16488..17465)
                     /locus_tag="PLO35_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOH78779.1"
                     /translation="MKKNILIILTGGTIGMKTSGNLGVVPGDELAGFLRQFPQLNNVA
                     NVDVLEYLNIPSPYMTPQKMLELAKLADLQVIDYDGAVITHGTDTMEETAFLCDLVLT
                     TRKPVVFTAAMRSGSELGLDGPRNIIDAVRVASHHESADKGVLVVMNDEIHTARDVVK
                     SDTGKIDSFMSPGFGPLGNVDPDMVVYHRAALYRESVWTDKIDTRVDLIKAVSGMDGR
                     LIDASLASGARAIVIEAMGRGNLPLETLPAIEKAISQGVLIVIASRAHTGRVLPEYGY
                     EGGGKHLSDIGAILAGDMQGLKVRLKLMALFGKYDDSDIVRRFFLQSRD"
     gene            complement(17462..18136)
                     /gene="queC"
                     /locus_tag="PLO35_03595"
     CDS             complement(17462..18136)
                     /gene="queC"
                     /locus_tag="PLO35_03595"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOH78780.1"
                     /translation="MDKAIVLLSGGMDSLVTAAVAVQECEQVCFLHASYGQKTQERER
                     QSFERLCQHYQPTDSKILDWSWLAEIGGSRLTDPDFASDRSLAESRIPDTYVPFRNAS
                     LICAAVAWAEAVNASRIYIGAVEEDSSGYPDCRKSFFEAMQKAIETGSRNLPLITIHT
                     PVIDLDKASIVRLGIQLNAPFELSWSCYFSSEEACGVCDSCRLRLKAFSLAGIADPIN
                     YRGRPK"
     gene            complement(18339..18923)
                     /locus_tag="PLO35_03600"
     CDS             complement(18339..18923)
                     /locus_tag="PLO35_03600"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOH78781.1"
                     /translation="MQQQPSCPWVGTNPAMIEYHDTEWGVPVHEDRKLFEFITLDAFQ
                     AGLSWSCILKKRDNFREALDGFDPSLIARYDDAKIASLLANPGIIRNRAKIKAAINNA
                     RVFLAIQEAQGSFDTYIWSFVGHQPIVNAWTEIAHIPVSSPESDAMSRDLKKRGMSFV
                     GTTICYAFMQAAGMVNDHLVSCHRYHSLQSAQMG"
BASE COUNT         4433 a         4785 c         5047 g         4601 t
ORIGIN      
        1 tcgctctgat cgcggccatg gaccgcaacc gggtcatcgg taaggacggc aagatgccct
       61 ggcacctgcc tgaagacctg cactggttta aaaccaacac cctgggccat cctgtcctga
      121 tgggcaggag gacctttgaa tcgctaccgg ggcccttgcc aggcaggctg aacctggtgc
      181 tcagccgcga tgcttcctac accgtcccgc ctgatgtggt gaagtgttcc agtctagaag
      241 acgcttttca ggcccttcag caaggtgctg cggagtcgca gactgccggg ctttcccatc
      301 cccccctgtt tgtgatcgga ggggccactc tcttctccca attcctgcct caggcagctt
      361 ttctatactt gagcctgatc gatgccgact atgagggcga tacctggttc ccggagtata
      421 actcactggg atggaatacc ttgtatgagg aacgcattgt atctgccagc ggcattccaa
      481 tctcgttcct gatccttgaa cggaaccagt agggcggcat atcggaccca tacctcagaa
      541 aagcgatttg ggtcgggctt ggtctgacta tccctgagct gtaccgattg ggaagaggcc
      601 tgaaactgga ccacgtgctt ctgatagggt gtcaccaccg gttaatccag cgctccggct
      661 gagttccggg acctccttag tctttgcggt gatggcaggt cagccacagc tccgcggctt
      721 ttaccacaat ctccatatag tcaaacgctt catccagggt cttgccaaaa cacagcaagc
      781 catgcttttg ccagatcata gcgttacgct cacccagaca gctcgcgctc gcctctgcca
      841 gttcaatcga tccgggtgga taggttggac agcaacatac tccttttgcc agatataaag
      901 gcaattccgg caatatttcc gccagttcca gattgaaaag tgtttcatcc tggaaaatcg
      961 gcaagtggga tatggcgatc acagaagcgg gatgagcgtg cagcaccacc ctgatatcat
     1021 tgccttgggc tctgaagagc tggtgcagct tgaggtgggc gctccactcc gaagttggtt
     1081 ttgcctcagg ggacaagctg tgccactgct ccgcctgggc ttcgatcaga cagagagact
     1141 gcatggggtc atcggctatc tggcgataac gcgatcctgt cctggaaacc agataagtga
     1201 tcggggccag gggatcatca ccgtagtctg tcaaacggat ggagacgttg ccagcgttgg
     1261 cttccgccca gccacgcgcc cagatgcggg tgccgatatc ggaaagcttg gagatcagtt
     1321 cctggtccgg gagtggggca gaagcatccc aagctgtatg catctgtagc accccttcaa
     1381 tgatagcccg gccccaggaa atcgccagcc tggggtcatc atggcgaacc gtttcgtcgg
     1441 tcagagtgat accggccgct ccatccagga cggcaaaagc gatatcggag acatccgatc
     1501 tggaaggaac ccgatcataa aacagcgagg gcagcatctg ggtggcgata tacaccgctt
     1561 tgccgtggcg ctgagcttgt ctgatcaagc gtctctgggc caaaggaatt tcccagagcg
     1621 ggacctgaga gcccaggtct cctctgccca ggagcagaat atctgattgg ctgataatct
     1681 catccagatt tgccagcgcg gttttggtct cgatcttgac caccagggcg ggtttatgat
     1741 cttttgccaa agcgctgata aactcgcgcg cctgaatgat ataagcaggg tgatttatgt
     1801 gggagagagc gatctgggcg atctggtgct ccagcgcgaa gcggatcagg ctcttatctt
     1861 tatctgtcag tgagttcgct tcagacggga ggtctggaaa gctgagatga gcgctcgcgc
     1921 gcggctttcc tcctctggtc acgcaaagct cggccccgtc aggcttcaag ctgatgatcc
     1981 cggcctcgat ctgaccatcc tgaatcagca ctctcatttg gggtctcaag aggtgccaat
     2041 cataaaaatc cgtgtgataa gctctggtat tattgccgat ttcgagggtc tggccatcct
     2101 ggagctcatg ctccggagcg tagccataga tcctgtattc aggtcccggg aggtccagca
     2161 tcactttcac aggcagttta gacagtttat ggagcacctc ttccaggtcc gcttgattaa
     2221 aatgagaggc attcaacctc acgatgtcca cacccggtat gatcagttca gcgaagctct
     2281 cagccatgat ctggctgggt ttgaaagtgg cgatcagctc tatatgactt gctttgatct
     2341 cttagtctcc gttggaatta cgttcgcggc aaatgcgctc tacttcacgg aaattgacct
     2401 taccgctggc catcatgggg atgtcttcga tcacgtaaaa ctgccgtggg attgcgattg
     2461 agggcagttc tttcttgagc ttggcgagca cggatttcat gtcaaaatct ccgttggcca
     2521 ccgccgccac cacatccgcg cctttggtgg gattgggaac atccactacg cagcagatca
     2581 cttcttccgg taaaagccgg ctgagtacat cttcgacctt gaccagagac accatttcgc
     2641 cgccgacttt cacaaagcgt ttcaatctgc cgcgatgcca gagatagcca tcttcatcga
     2701 taattccgat atcgccggta tcataccagc cgttacggat cctcagagag gtttcttcca
     2761 gatcacccag atagccttcc atcaccagat cacccttcac catgatcttg ccttcctgat
     2821 tggggcccaa gactttatcg gtatggatgt caacaatccg gacatggaca ccaggtatgg
     2881 gctttccaat ggaaccggtc ttgtggactc cgggatagtt tgatgagatg accggggagg
     2941 tctcggtggc gccgtaacct tcaaagacag tgatattgtg tttatgcaaa aagccttcgt
     3001 aaaccttcat gggcagcttg tcggcccccg caatggcaaa gcggatgctg gcatagtcac
     3061 caggctggga cttttgcaga tatccataga agaatgaggg agtggcggtc atgaaagtga
     3121 ccttgtactc tctcaccaga tcgctgatag tcttatattc caagggattg ggataagtga
     3181 ccatggtagc gcccaggatg gtcggcagcc agaaattggt ggtgaggcca aaaacgtgga
     3241 agagcggcag catgcagatg aaagtgtctg tgtggtccag cctcaccatc tgcggaacgg
     3301 cgtccacatt gtggaggatg ttgcggtggg agagctgcac agctttgggt tctttttcac
     3361 ttccactggt gaagaggatc acgctcactt cgctggcggg accgctgtgg accatgcctt
     3421 tgaggatgct gaagggcagc ttggaaatca gggccgcctt gagcttggca acagcggtga
     3481 ctgaggccag gatatcctcc acgaagacca tgtggtcgat agctttgagg tcaagctttt
     3541 ccagcagttt gcgagaggtc aggatggtct tgaaattgca cttttctctg gcgtagcggc
     3601 aattgtccag cgcgccggtg gagtaattga tcatgacggg aattttgccg ttcataaggg
     3661 tcccgatcac gcccagcatg caacccatgg tggtgggcag cataatgccg acatacttgt
     3721 ctttgatctt gcctatgtgt tctttcagga tcaacgaggc gatcagcatg cgtgagtagg
     3781 tatagtcttt ggaggtcgcc ttgtcataga cggcgatcct gtttccatgt tgttttgcgg
     3841 tttggataaa gcgttggtgt aattgcagca ttcaaaatct ccaggcagat agttctatgt
     3901 gccaggcaac aaaaaccatc tggaatagtt aatcagttca cactaattgt gtatggcatt
     3961 catgcaaaag ccgtgccatg cggtaataaa acttggattt cctttagttg atgatatgca
     4021 gccagtcctt gaggaggaat tccagcctgc cgatcaacag agacacgcgc gccatgacgg
     4081 tatacccaaa agtggcaccg aagctaatca tgaggaaata gacccctatc ttggagggga
     4141 ttttggccag gccttcctgc tttttgctaa aatagaagaa atacacccca cagagcgtcc
     4201 cgatgatcac catcaggttg cctatcacct ctggaatgga tgcgttggca aaaggattga
     4261 tcatagtggc gccaacctgg ttgatcaggt cgcttttcag gtaacgcagc agactgatgg
     4321 cggcactggt gccgatcacg atagccaggg gataacgact gatccactgg gccttgggga
     4381 cgatgcgggt gagcatcatc aatcccagcg cgaaaggtat cagcaggatc agattaccgc
     4441 caaagtccga aaacagcttg gtaaacagat tgggcaacag agtggtgtag atcacataaa
     4501 tcaatccgta acccgctgag agaccgacaa atatctgttc agccagctta tagaagggat
     4561 tgtccttgta gaggaagcta aagatgcaga aggtgaaaaa ggcagccagc cagattccga
     4621 ttacttgcat gatctcaggc attgttacct ccaggtttgg gagcggcggg gtttctgcgt
     4681 ctccaggctt tgatgttgcc gagggcgata aagagcagaa tcacgatatg ggccacggac
     4741 tgagcgtcca tcttggtggt ggcgggccct tttttatcga tcagcatctc atattccgag
     4801 gcggctttga ggccaccgag gagaccggta agctgatgct gatcgtttac atatggcaaa
     4861 aatcccggag cgctcacagc cgtggtgccg cccgtgacgg gaattttatg gacgtcatgg
     4921 gcgatcatga tccactctct cagtcccggc acgcctgagg acagagaaga aatccaggcc
     4981 aggtctttga ggtttctgat gtccttgagc atggggagct tatcccaatc ggtgccggat
     5041 gaatctttgg ggtaaacgga tttaatatct gagcccatgg cctgaattgt gaccaatccg
     5101 cctactttgt agcccagatt cacatagtcg acaccgtatt gtttatcctt gaaaacggga
     5161 ttcttcaacg cttcctcaaa acactgggca gccatctgag gcccttgagg ccacagggcg
     5221 gtggcgatca ctttctgccc tttcgaccag gcatgttcga tctgagctac tgccatggga
     5281 tgcagttctg tgatagtgga ggcttcataa tcgaaagaga tcagcccata gctgctggcc
     5341 gcggtgtcat ctaccagctg gtgggccgcc ctcacaatag gagaaacctc gctcgtccag
     5401 ccaatcggcc agatcagggg aatcatcacg gccagaaaca caatcccgaa gataacgcgg
     5461 cgttctgtgt gggagctggt gttcacgctc atttgtcgcc tcccagataa gagcgctcga
     5521 tacccagaat gattctcagg ccgctaccga tgatcccaag cgaagcgctg atcatgatag
     5581 cccgctgggc cgcggaattg gggtattgca tgatatagtc ggccaggttt ggcaggtgcc
     5641 agaatttgag gctgtctggc agccagcccg tcaacatggt cccaaatccc gtgcggccga
     5701 gtattacaat tacggcggcg atcatcagca gattggattc cagagtgcgg ataataaaag
     5761 cccggtaggc cgcggaagcg ataaaaaagg ccaatagcga aaaaatcgtg gcggacagag
     5821 gcacaaaagc gttatcgtaa agatagtcaa agggtttctt gcccaaccag gagctcacca
     5881 ggctgccctg tcccagcatc ccaggccgtt cttccattcc ccacagggct cccatcaaca
     5941 ccatcagcgc gaagctgatc atgccggcca gataatattg ccagtcctgg cttttagaac
     6001 tgatcctgga ggcgttcatg ctgagcagag atatcagccc caacagcatg gcaaatcccg
     6061 atatgatcat aaaccattcc tggagggtgt ctacaatcgc gctgaaggga cgatgaggga
     6121 taaattccga gaataaaacc accagcccgg cgatgaatgc caggatcaaa ggaaggttgc
     6181 gtttcatgat ccagctccca ttaaaaacgt tttaaaccat cccagtccag caatttcaag
     6241 ggctactccg atcaagatca ggatgattac taaaagtttg ccaaagtcat ggcccttgag
     6301 gctgccaacc tgttgagggt ctctggaaag gtaggccgag gcggcaaaaa gctcttctcc
     6361 gattagagtg tagtcgcaag aaaccacaaa gaacggcaat tggtgagcat tggccgttcc
     6421 ggctgtctga atggcaccgg tactgagacc tgtttccgcc aggatcagag attcagcgta
     6481 gaacgatcct aaaaagaaat tagcggcggg ttgctctcgc acgatgattc cgtcgatgcc
     6541 ggccacatag ccaaactgat cgtcggtgag gtaaaagacg cggtcttcct tgtaggaatc
     6601 agctcttcca gctttgagat aggcttcttt gaccacttct ctagcagcgg aaagcaccat
     6661 cgcaaagcga cagggaacga tcagctcggt atcgtattcc gcggcccggg tggccaattg
     6721 acccaggatg gagaggctgg cgattgtttg aatgtcatcc agctcagaaa tcccaggaac
     6781 aaagaggatc ggcttgcctt tttcagtggc acggccgatg gcttcttcca tggaatcaag
     6841 acctccgatc ctgcggataa aaaagtcctt tccacgtctg gcagccagga tgtaatagac
     6901 aatcgcgctg ccgatgatca gaaagagcag cagataggcg agtctgtcca tataaaacca
     6961 gttttctttg ggttgggcca aagcgctgag aataatgctg tctccactgg ctttctgata
     7021 ggctatcatc cgatatctgt attgcttgtc cgggtttagg tcaccatcgg tataggaacc
     7081 ctccgggctg taagaggcct ccaaatctac gaaaagtccc agagaatcag ctttctgcag
     7141 ccgcagggag tcaattcctg caggcagctt ccagtcaata atcaggcttt cacccttgtc
     7201 ccagggtttg tcaatcacag ccaggctagc ttctgaaata tctggaggca cgagtctgga
     7261 ggccgggaca attgcatcgg gagctgcctg cgctcccagg gccaaggaat gaagtgccaa
     7321 caggagcaca ataatcgcga aacgcgtcat atccacctct gagaatagct cataaaaata
     7381 caaaacccgg cctggttgtg acgcaggccg ggaacatcat taacctgaaa cggtaaagtg
     7441 cgattacctt gggcatcgcg actattgctg ccaggcggct ttaatctggt cggtggcttc
     7501 tatcagctgt tggagctgct ctttgctgta ggttttagtg gtatcggcgt tgaggatgtc
     7561 cttcactttt tgcaccagtg ggacggcagc ctggtaggga gcggaatctt ttatggcagg
     7621 ggtggagacc agaccgaaat tggcactcag ggaattccag gtgccttttt ggttcactgc
     7681 gctcgtattc tctgccttgt aattgtttgc caacaaacgg gatacattgc tcaatgcttc
     7741 ggtccagcca cccaggatca tcagtgtata atgttcataa ttctcaagct cccagagctt
     7801 gtcttccaca gatttcttta cctggtccag agaagcatcc agatcttccc atttctcttg
     7861 cgcgatcagg gtactgaggt tagcgcccat gcggttgatc tcgtcctcta agttcaggag
     7921 agtggtaaga ttcatcatct cctgagagat ctcagtcagc ttggccttgt ttctggcttt
     7981 ggcggccata accgcgtccg cggtcaggac tcccagcgcg aaagcgttac gggaagcttc
     8041 ctgtttggtc ttaaaaagtt tctgggggat tacggcggaa aaatccttgg ccgggacctg
     8101 gtccagagtc cgaaacactt ctttaaaact tggcagcggg gcataagtag ctacttgcgc
     8161 tttgatctcg tcatcgggga gcttggcagc ctctttattg cccttgcatg atacaattgt
     8221 catggccaat agaatcgcca tcaggatcag taaagttctc ttcatgtttt ctccttttac
     8281 tctttaaaat aatactttag cttcaagatg gtgatgaggt tgaaccccag cacatacagc
     8341 aatatcatca acacgttgta attccaggta cgatggattt taccctttat gagcttgtag
     8401 ctggacagga acacattgcg ccgatcaacg tgaaactggc cgtccatgag gcccacgctg
     8461 gcgttcagat tggcgttcgt caccttggtt gggtcccatt tatcagccac ataatcgcgt
     8521 tgattggaga ttcccgcgat cagctttcta tattgggcag catcaagacg attatctttg
     8581 aacatggcat tatatgtcaa ccgctttttt tcgatctctg ccagtttacg gtggaaaaca
     8641 gtgtttttgg cgtaggcggt ggtgagacct tcgaaaatcc agcgggatgg gatcaactga
     8701 accacttcgg gaacagggtg tcgctccagg agtgtgaggc gccggttcat ccgctcatat
     8761 tcgatcaccg cgcctcccag gatgatctgg gggatcagga ggagcggtag caggttgatg
     8821 atggatttgt tgtctttgag aaaggacgat accgtgagtc ccagtgaaaa tcccactatc
     8881 gaggtgagca gcaggtaaag gatgctgata ccccacatac ctttgatctc aagcaccaga
     8941 gacgctacca ggacataaag cagcacctgc accagcgaga aaaacgtgag cgcgatcagc
     9001 ttggatgcca gataaaaact caccttgagg ttcatgagtt tttctctgag gataatcttg
     9061 cgttccgaga ggatttcttc cacgctgttg gacatgccca taaacatgaa ggcgatgatc
     9121 gaaacgaaga taaagatgcc aatgttgttg ttgtcgttat agttataggg tcccgatcga
     9181 ggggtatgcc gtagaatgaa agagatcacc agccccagca ggggggcttc cacaaaagtg
     9241 atgagattgt tggttcggtt gcggagcttc atagtcaggc tgcgggtgat gaaagccagt
     9301 aaatgaacaa gtctgctgcg aaaatccagc ttgcggtgtc gtcgtttggg gatgctggtc
     9361 cgggcaggtg gatcagccgg ctccagctgg attatctcaa agagcatctt gcgtttgtat
     9421 ttatctctcc aatattcggg aggaaacttc cgctttactt gcaagagctt gtttacctgt
     9481 tcatagaccg gggcgttgct gtcgtcgtat tgaggatagg tgattacttc atacatgtaa
     9541 tcggagttgt gaagctgctt tttgcgctcg atatcttccc ggcggtagcc aagctgggcc
     9601 agttcgtaat cgaagtaatc gaagcattca tccggagggc caaaaaaagc ctgccggccc
     9661 cccaggtcca tcaagagcac ctggttgaat ttccggaaca cgctgctgtt gggctgatga
     9721 atcgtgagga tcacaatctt gccctgcgcc gtgagcagtt tcaggatgtc gatcacctgt
     9781 tccgcgtcac cccaggaaag accgctggtg ggctcgtcgc agacgatcac ggtaggttca
     9841 aaaagcagtt ccagcgcgac attgaggcgt tttctttctc ctccggacag catcgtgcgg
     9901 cggggctctc ccacctgggt atctttacga tggctgagat ttacctgatg aaggaggttt
     9961 tcgatctttt gcttcagcga ggcagctgag atattgggca agcgcaacct gccacggtac
    10021 cagaggtttt cgtatacggt cagattggga tagagcagat catcctgagg cacgtagcca
    10081 aaaaactgtg agtagtagct caggttgttg taaaggacct tgccgtcgat cttgacctgt
    10141 ccgtaggtag gtacgagctc gctggtaatc gtctttacga gcgttgattt cccacagcct
    10201 gattggccca ggatgccgat cagttctcct ttccggatat cacaggatat accgtcgagg
    10261 gctagcttgc catcctggaa atagtgtttg acgtcttgaa tcgtgaggct ctgcacctca
    10321 aagggggtct ccaccagatc gtagaagccg ttgatgcgga agtttcggcg ggcgatagtg
    10381 aggatgtcgg tattgagttt aaactgagtg agttccttca cccgctgctg attgtgaaag
    10441 atggtccatc cacgcttggg gggatgcagg taaaacttgc ctttttccac ttcaattctg
    10501 gcaatggcgc gctcggtctc tactctggaa agatagaaga aatcccgctc ggcgatcaga
    10561 aacagctccc tgggcaccat cgtatcgatt ccaatgctgg agcgctccct gatcacgtct
    10621 ctcactgtga atttggcata accctgaatt tgcaggacat catcgtaaaa gagctccagt
    10681 ttgcgcccgt gaaccagctc tttgtcgttg acggtgatcg tgcccccgtt taccagaatc
    10741 gagtattgat gccggtgccg gctgatcagg aaatcgtagt ctggcttctt gaactcgata
    10801 tcatcatcat cttcggccac acggtaaatt ttccaaaggc tttcgatgtc ataggccagg
    10861 ccccggacct ccaatgtccc ttccggtggt aaaaggatca cacaggaggg cttgaggtcc
    10921 agtccgttga gcttgctcgt acatgaaaac gaggtggaaa gaaagagaga tttatcgatg
    10981 ccgaagagga tacagtccag ctccccgatg ccgggatctt tgaaccgcat gtctgccaga
    11041 gaggaagatc cgatgctcac atagtctgta aacagagagt cctgcagatg ctgatactgg
    11101 atgtcgtgcg ggatggctac acattcctga atacctgatt caaaatggtc catcagctcc
    11161 acaaaaggct cgggactaag ctcgaatttc ctgcaaagag cgataatccc tgtaagctcg
    11221 gagatttcta tgctgttgtc ggtattggcc agaataatga ggctgaggat gatcctgatc
    11281 ttgtccagga cgttcagatg ctggttcaga tattgactga tctggccaaa atcatcctca
    11341 gtttccatga tgttgcggac atacacttcc caggagatat tgacgtccct gaacaggttg
    11401 atgagcagag agtagaggat atcgatctca gaggctgata tttggtcttc ggatttgatc
    11461 acatgcacat agaggcgcgt gatcatgtcg atggtctttt ttagctggcg atcaccaagt
    11521 ttgaggtttt tatcactttt tggcattatc tatccaatgc gctgcgtccg gcactcatca
    11581 catatatggt atcggcaaaa tcgctgatgt agttcaatca aggcttgctg atagaccgct
    11641 ttggagttga tcagtttctg ctggcgggga tcagtgtgat cgctcatcat gcggatcagt
    11701 tgattgcctg ccaggccgcg gtgtttgaga taggcatccc gggtttccgt cataagcaag
    11761 ttgtcagacg ttttttgacc ccataagtaa aagaccggca gacagatgtt gacgtatatg
    11821 gaactcaaga ccccctgacc tggtctgggc agcatctctg ctcccggcag cagggttgag
    11881 ctaaagagct ctgtaaaacc cttatcccat tgtttttgag tgcctcgggg atgcaactct
    11941 ctgagcagcg aacttaaaag gccatcctga gcgtagcggt ggaccatggg tatcaatgcc
    12001 agcaaccggt aaatggggtg attagctggg cggatccgaa aaagctgcca gtcgatggaa
    12061 agcctttccc caaaacggtt ttgagcttgc caggcctgct gcaatatctc ggtcaactga
    12121 tccgggacca atttctgaga cttggccaac aagccactgg caatggcgta gatggccagg
    12181 agatcaatca tagagaggcc ttcagcgctc cattcgagca tcctggccag gggaatatcc
    12241 tgggccagta acagcatggc agtcttgttt ttatcataac ccatggcctc aaacagccct
    12301 tcgtagagaa tctggtcaaa accgctgaac atcagtgagg cattgaacct gttgacctta
    12361 cccagaaacc tcttgaggcc aatatcgcgt aagatcagac ccagcctgtc gttgtccaga
    12421 gcggacaaca agtcgcaata agctggccgg cctttaggca ggcggggttg atgctcgcgc
    12481 agcagcttgt cgatatcttc actgagctgt tctttcagct ccaggatttc aattgcctca
    12541 ccattctctt tgatggtgta atcgcggctg ctgttgtgtc tgaaaaccac atgcagggcc
    12601 actttgttat aaaaatgatc ttcagcgtga ttgtgagcat gccagtcggt ggtgagctga
    12661 tggatctcca catctcctct caccgccaga ccgtctatca cgagtcctga gtttagaaag
    12721 tcgggtccgc gattggtgtt gtattgaccg ctgtatccta ttctgagatg cttcccgctc
    12781 accgtcgcca gttccatctt caggtgcccg ccatcccaga tatgatgcag aaaccgctca
    12841 tccattagaa ataccggttc aacatccggt agcgctgacg tgcggatgcc gatctggaat
    12901 aggtttgagg gctgtatttg atgggactgc tcaggattga taccagcttc atggattggt
    12961 ctctgctgat ccgggagcag cttcttccat aatagtagag tgaggcggtt tctattccat
    13021 agatgccctt gccccattcc acatagttca gataaagctc cagcatgcga ttcttggtca
    13081 tcaggacttc catgatcacc gttacctgag cttccaggta tttgcgcaga tagtttctgt
    13141 ccgtggtcag gaagatggtg cgggctactt gattgctgat tgtgctggcg ccaaaacgaa
    13201 tttttccgga agtcttgttt cgcttccagg cccgttttgc ctcttcaatc gcaaaaccat
    13261 aatgcctgta gaaattgccg tcctctacca cgatggtcat atcgaccgtg gaatcaggga
    13321 tcttttccaa gtcgatatat tcacgtttgt gccaatcaaa cccccggatc atatacctct
    13381 gcagcatcaa gggagtgatg gggggattga cgaagatgta gagcagacaa aaaaacgcga
    13441 ccacgcccca aaaggcaagg tggcaaaacc ataatagcct gataaacctg atcagccagg
    13501 gccggcgctt tttaccggtc tgtgaggagg aggaaagttt gcccaaacga tgtccctgaa
    13561 ggctgaccca ggagggccag ccaggactaa agatcgagaa gggttattct gacttgttaa
    13621 gtttgtttac caaagcggcg acgcgagatt tgcggcggga agccgtgcgc ttgtggatca
    13681 cgcctttttt ggcggcttta tcgagctggg agtagatctt gtccagcaga gcggttttct
    13741 caggggcagg aagctcggac tgcatctgct tggaaagggt cttaagggtg cgcttgacat
    13801 agttgttgcg cgcctgtctc ttcttatctg tcttcatccg tttgcggggt gcggcatgtt
    13861 gtggcatggt tacctcttta tgtttatgta ggttgtgata ctggctggca gggcagtgga
    13921 aacagtctgt caaccattca cacagcgctt gaagtcattg gggccggata gatccaaccc
    13981 tgactcttca cagctcatta gtgtttgtca cgaaattata tggttgcctt ttttgtcaat
    14041 accaattttt caatgcaagg catccagcag tatcctggat tgagattctg agctcaaagc
    14101 tgagatgtct ctatcgtaga tcacccgctc gcccaggctg tgtctgcgaa gcaaatccgc
    14161 tatccaggcc ttggcggcaa gcacctcgtc ccggtccacg caaagcccag cttgataagt
    14221 gcttatcagt atctcggctt ccccacccct ggggccgatg gccaggatgg gtactcccac
    14281 ccccaggtat tcaaacagct tggtggtcag catgccctca aaaccttcgt agcgattgat
    14341 catcataagc aaaagatttg atctggccat ttcgaccagg gctgttccat gcggcagata
    14401 tccgattgtc tcagaaacgt cccccaggcg atcatcaatg gagcgttgtt gttccggact
    14461 gagcctggag ccgataaacc gcaccctgat gcttggaagt tccctgaaat ccgcgaggat
    14521 agcaagcatg gtctccaggt cctgcccagc ggtgatttgg cccacataac tgatcaaaaa
    14581 atcctgaggg cctggcgcag tgatgccagc cgtgggaatt gtcccggctt cttctttggc
    14641 tctcttgatc tcctgggggt cgaagccatt catcagcaag agtttcttgc cctctggaag
    14701 agtgttgagc agataacggg agatcaccag attgagatcc gctttggcgc agacaagtcc
    14761 ttccatgtat ttatgagcgc tcaggctaaa ttgggagggg ggattgagat tcaagtagtg
    14821 gatcgtgctc cagggatcgc gccagtcagc tatccacctt agtttgaagc tctttctgag
    14881 tggcagcccc aacaggtgag tggagtgggg aggacctgta gtgatcaaag tcctgatccc
    14941 acgttcctga atgatagctt tggcatcgcg ataagcggat ggattccaga atatcctgag
    15001 gtctggaatg atgagattga gcctcaccca gatcaagacg cgccttaaga gagggtcttg
    15061 cgggctggat ctcaaatcgc cgtgcggaag ctccgagcct ttgcccagca gcagtttcca
    15121 caatctgcca agcttggggg cgggagtcct gtggatatta agttccgagg gcaagcttgc
    15181 caatagagac tcatccagct gaggataatc tccccctgag gtagtcaaaa catgcacttc
    15241 ccatccggcc ttcagcaaat agggcaataa cctcagccag cgttgtaccg cggctccacc
    15301 acagggtgga aaataataag tgatcagcag cagacgacgc tggttcatta gctttcactg
    15361 gagttatgca ggctcttgat aaagtctgcg gaactatcgg cagaagccag cttcccagag
    15421 ctattgacac caggcccgat aatgatcttt cccagtctgg accagctgaa ctcagcgcct
    15481 ttcttgctca cgtagggagt cattgtttca tagagggagc ctttaaaata cctgatcaat
    15541 tcgcgtgcca gatcttccgg acggggttca gggacagcat tcaccagcag gcccgtcctg
    15601 ccttcatcca catactgctc caacccttcc agccgcgagg ccaggactgg cagatcaaac
    15661 gcgtaactta gagcgataac cccgctttgg gatgcactgc gatatggcag cacacagagg
    15721 ctggcccgtt ggaaaaatcc tgccaccgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnna tgttgtcgca tcgctccaga aacacttgtg ccaaaaaccg ggctcccagc
    15901 cagggctcat gcggcaccag attgtgcaac agcgctgtta tcctgacgcc tttgagagca
    15961 cggcagatca tgatatagga aggggagaag aaggggataa aatatgaaac gatcaaaatg
    16021 ttgggttgat aatccctgat cttcttaacg gcttgaggcc aggtgtcagg ccggtagggt
    16081 atgtagcagt tatcgatctc cagtttggaa gggaggataa actccgcggt ctgggatttt
    16141 cccgggaaga tcagagcggg atattgcctc cgaaacccaa acattttcac ctggtgtcct
    16201 tccgccagcc aggtggaagc taaactgagc gcaaactgcg atatcccacc tctcagaggg
    16261 ggggctggtc ccaaaaatgc gattttcatg tatgcctcag tcctcttgtg cgtttcccgc
    16321 agattacgca gatcagcgcg cagatctcgc aaatatttaa taagcatggt tttaaaaagg
    16381 ttaaaaaagc tgagtaagtt gagtaagatg agggggctgg acactttgag ctgataatct
    16441 gcgaaatcag cggaaagatt tgcgtaatct gcgggaaata aaccatgtta atcccggctc
    16501 tgcaagaaaa aacggcgcac gatatcggaa tcgtcatact tgccaaacag ggccatcagt
    16561 ttgaggcgaa ccttcaggcc ttgcatgtcg ccagccagga tggcaccaat atccgagagg
    16621 tgtttaccgc ctccttcata accatactca ggcaggaccc gacctgtatg cgccctggaa
    16681 gcgatcacga tcaacacccc ttgggatatg gctttctcaa tcgccggcaa ggtttcaagg
    16741 gggaggttgc cgcgccccat agcctcaatc acgatagcac gcgctcccga ggccagagat
    16801 gcatctatca ggcggccatc catgccagaa actgctttga tgagatccac ccgggtgtcg
    16861 atcttgtcgg tccagacact ctcgcgatag agggccgccc gatggtagac caccatatcg
    16921 ggatcgacgt tgcccagcgg tccaaaacct ggagacataa aggaatctat cttgccggtg
    16981 tcggatttga ccacgtcccg ggcagtgtga atctcgtcgt tcatcaccac caggactccc
    17041 ttatcggcag attcgtggtg actggccacc ctcacggcat cgatgatgtt gcgggggccg
    17101 tccaatccaa gctcactgcc actgcgcatg gcagccgtga acaccaccgg tttgcgggtc
    17161 gttagcacca ggtcgcacag gaaggccgtt tcttccatgg tatcggtgcc gtgggtgatt
    17221 accgctccgt catagtcgat cacttgcaga tcagccagtt tggctagctc gagcattttt
    17281 tgaggagtca tgtaggggct gggaatgtta agatactcca gcacgtccac attggccacg
    17341 ttattcagct ggggaaactg tctcagaaat cccgccaact catcgcctgg caccactccc
    17401 aggttgccgg aagtcttcat gccgatggtt ccaccggtga ggatgattag gatgtttttc
    17461 ttcatttggg cctcccgcgg taattgatcg gatcagcgat cccggccagg gaaaaggctt
    17521 tcagccgcag acggcagctg tcgcaaaccc cacaggcctc ttcagaggaa aaatagcagc
    17581 tccaggaaag ctcaaagggg gcattcaact ggatacccag gcgcacgatc gaggctttgt
    17641 caagatcgat taccggagtg tggatcgtaa tgagtggcag attgcggctg ccggtctcga
    17701 tcgctttttg cattgcctca aagaagctct ttcggcagtc cgggtatcca gagctgtcct
    17761 cctccacggc accaatgtat atcctggaag cgtttacggc ttcagcccag gccaccgcgg
    17821 cgcagatcag actggcgttg cgaaaaggca cataggtatc ggggatgcgg ctctcagcta
    17881 agctccggtc agaagcgaaa tccggatcgg tgagccggga gcccccaatt tctgccagcc
    17941 agctccaatc caggatttta ctgtccgtgg gttgatagtg ctggcagagc cgttcaaaac
    18001 tctgtctttc ccgttcctga gtcttctggc cgtaggacgc gtgcaaaaaa caaacctgct
    18061 cgcactcctg gaccgcaacg gccgcggtaa caaggctatc catccctccg ctcaataata
    18121 ctatggcttt gtccatttat ccttacctgt ctataattaa tcatcagtgc tcacctaccg
    18181 atcctgctga tgctacccaa gtctgcgtaa agatcatact caggaagggc ctgattgtgt
    18241 caagcgcggc gtttcatgct ttggtcttta aagatagctg ccgcttgatg agaggcattt
    18301 tcggcttcaa cgcctcggga tgctgaaggc aagatagatc atcccatttg tgcggattgg
    18361 aggctgtgat agcggtggca gctcaccaga tgatcgttta ccatccccgc cgcttgcatg
    18421 aaggcataac agatggtcgt gcccacaaag ctcatacccc gtttcttcaa atctctgctc
    18481 atagcatccg attcaggact ggaaactggg atgtgggcta tctcggtcca ggcgttgacg
    18541 attggctggt ggcccacgaa actccagata taggtatcaa agctgccctg agcttcttga
    18601 atagctagaa aaactctggc gttgttgatg gcagccttta tcttggcccg gtttcgaatg
    18661 attcccgggt tcgcaagcag gctggcaatc ttggcatcgt catacctggc tatgagcgat
    18721 ggatcaaatc catccagggc ttcacggaag ttatcgcgtt tcttgaggat gcaagaccag
    18781 ctcagaccag cctggaaggc gtccaaggtg ataaactcaa agagcttgcg atcttcgtgg
    18841 acgggaacgc cccactccgt gtcgtgatac tctatcatgg caggattggt ccccacccag
    18901 gggcaactgg gttgttgttg cattttttcc acctttgaga ttttagtccc tgtaaacaga
    18961 cagccg
//
LOCUS       DAOSFN010000033        18958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2358_length_18958_cov_9.271703, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000033 DAOSFN010000000
VERSION     DAOSFN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 18958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18958
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2358_length_18958_cov_9.271703"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1759)
                     /locus_tag="PLO35_03605"
     CDS             complement(20..1759)
                     /locus_tag="PLO35_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOH78782.1"
                     /translation="MDKFTAQKQIAGLAWLRRDIIGRNMPIPTPYGERPLVYADFTAS
                     GRGLISIENYIGKVLRFYANTHTEDDFTGKTMTRLLHEAEHTIKDAVNAGPKGKIIFT
                     ESGTTGGIVKLLQILGVYWPPATRERVGQILKSCLIRNPRGIDCNQALHDFMHHNKPI
                     VFVGPYEHHSNEILWRQTLCEIVEVPMNKHAELDLERLEELVSDPEYANRLKIGSFSA
                     ASNVSGLKTPVYEVARILHRHGGLACFDFAACAPYVKIDMNRDAESYFDAIFLSPHKF
                     LGGPGTSGVLIFNADIYPSALPPTVPGGGTVDYVSPSKEEYVHDIETREKPGTPGILQ
                     ALRVALAFQLKDKVGHDVIEDLELYYYQKFMSAFEGDARITFLGVLDADKKVPIVPFN
                     IRHQDRILHPKFVTRLLNDLFGIQTRAGCSCAGPYGHYLMGIPQQVSDYYRCLIVNAR
                     YSGIKPGWVRLNLHYAMEECEVDYLIDAVKFVVEHGYKFLSVYEFDIHSGEWTHHANH
                     ILPALALDIEAAFAGVQDHHQGPEDACDLYRAALQAASEEAARLPQAFDLLSFEPELE
                     ALMYFYVNNMRKR"
     gene            1878..2783
                     /locus_tag="PLO35_03610"
     CDS             1878..2783
                     /locus_tag="PLO35_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78783.1"
                     /translation="MRIPAGLAILSHGEDILPAVDWSVYKRFDRVVNYIHKDRLLSVC
                     AASIPSGVDHLVLNVDDLEWLQSVQTLTDRVVLNGVAFPLDPCKLYRVPPLPLPASFD
                     HILTLESWLRAQAPPLSIPRLLHEAYRPGSFDAALGRVFEEGIDLLESREFLQATRLL
                     KGCGFGSTPSGNDFMSGYLMAIAWLTQAEWQTTGLPESGPSVGKRGRLPLHRGSQKNR
                     LSEIADLILYEILGTDPLCDAFARRARALKPDSDWKELLQVFADQSADARPALEAVLS
                     HGSTSGADALAGFFMGLRSYHMEVK"
     gene            2780..4300
                     /gene="fdrA"
                     /locus_tag="PLO35_03615"
     CDS             2780..4300
                     /gene="fdrA"
                     /locus_tag="PLO35_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HOH78784.1"
                     /translation="MIVKAMVIQGRYLDSVKLMLISRSLSSLPGVQDAVAILATQENR
                     GILKATDMLVDEVLHAAETDIVVVVKARDDDSALAAIQEASKTISDPSPTNGSRVRLA
                     LGMDEALKQGSDLCLISIAGRYAAREARKALQAGLHVMIFSDNVSLEDELSLKRLALS
                     KGLLMMGPDCGTAIINGVPLAFANAVPKGGIGIVSASGTGLQEISAGIANRGMGITQA
                     FGTGGRDGKEAIGGLMLKACLKYLIADPATQVIVLIAKTPHPEVRKELWELIGGTSKP
                     VIVNFMDHIEHPHLDNLHYAVSLQTCAHLACFHYARLHGLKYQETHEALPLHPTIQSD
                     KRRWLRGLYSGGTLCAEARLIYKHELGGEPYSNITNRADRQLKDPWRSLEDSLIDLGS
                     DEYTVGRPHPMIDLSLRIKRLAAEASDPEVAVILLDVVLGYGAHPDPASELAPAIATV
                     DKDILIVCSVLGTAGDPQDRASQMSMLREAGAVVFSSHHAAADYAIQAIKAIRGIS"
     gene            4297..5736
                     /locus_tag="PLO35_03620"
     CDS             4297..5736
                     /locus_tag="PLO35_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOH78785.1"
                     /translation="MSLFKLPDGKPRVLNLGLEAFAIDLESQGVEVTRVSWKPPMELA
                     PGALDKLLAARETIDAANAKALEIIQNGKPLLVGMGIARELIPGMHDDLILHAGPPIT
                     WDRMCGPMRGAVIGALIYEGRAKTASEAEALASSGRINYAPCHEHSAVGPMAGIISAS
                     MPVFVIKNERYGNLAYATQNEGLGKVLRYGAFSAEVIERLKWMESVLYPTLKRAVEHL
                     GQIDLMSLIAQALHMGDEVHNRNRAGTSLLIRQLAPALLKTMTNVENTARVLDFINGN
                     DHFFLNLSMPAAKCILDPAMNIPNSSVCVTMARNGTDFGIRVSGLGTSWFTGPALVPD
                     ALYFPGYTKADANPDIGDSAITETGGWGGFAIAAAPAIVQFVGGTAADALSYTNTMYE
                     ICAGESTRYQIPALDFRGTPLGIDVLKVVLKNITPFIDTGVAHKDPGVGQVGAGVVQA
                     PPEPFMRACEAFADLMDRQRSSFASSTET"
     gene            5765..6577
                     /locus_tag="PLO35_03625"
     CDS             5765..6577
                     /locus_tag="PLO35_03625"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOH78786.1"
                     /translation="MQMDQFLDFMQGLKMYDLTQRLSIHTPPWPSYMPLGIQYFKRIA
                     GAHMGQGANGQIITTSNHVGTHIDGEIHFHASGRTIGEVPMHEWVGPGVVVDISDSVG
                     DYDLYSPELLMKKADIRSGDILIINTGYHKYAWDQPESDELRYFVKHPGPDRNFHKWA
                     LEMKIKWIGVDCGSADHPMNTIIRTWHPKSFIEAENKLKATYGMSWDEMFPTEEYYQV
                     MHLKLFPKKLVHAENLGGDIAQVSNKRLWIGLFPLRGIELESSMCRVIAFEP"
     gene            6823..7719
                     /locus_tag="PLO35_03630"
     CDS             6823..7719
                     /locus_tag="PLO35_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOH78787.1"
                     /translation="MAIAPTFEYARPRNLEEALRLKDQYRERALVLAGGTDLIANLRE
                     DMIHPELLIDIKDIPGLDKIVIEAGTLKLGPLVTFTQLIEFGPLGKSFPMLLDASRTV
                     ASVGIRNRATLVGNICSAVPSLDSAPPLLCHEAVVHCLSLEGERHIRIDDWFTGPRKT
                     ALKPDEIVTGITLQPVFHSGSGIYLKLGRYGGEDLAQAGWGILIDGANHYRVAHCALG
                     PVPARASKIEALLDGNQLNEALIQQAMDLVPEEISPITDIRSSKEYRTHISKVILKRG
                     LLAARERLAGKDIDPRTLLGGI"
     gene            7719..8183
                     /locus_tag="PLO35_03635"
     CDS             7719..8183
                     /locus_tag="PLO35_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOH78788.1"
                     /translation="MKEIHLTLNHEERSAWVETNETLLEVLRDKLGVKSPKCGCERGD
                     CGACTVLMDGKAIRSCLVLAVEAEGHSILTVEGLSPDKLTALQETFIEFNSFQCGFCA
                     PGIILAITELLESHPKPTLEEIKESIAGNLCRCTGYTPIFDAVMAHIQREDK"
     gene            8180..10513
                     /locus_tag="PLO35_03640"
     CDS             8180..10513
                     /locus_tag="PLO35_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOH78789.1"
                     /translation="MKDFKYIGKDVVRIDAREKISGAAIYGDDIDFGPKLLYAEIVAS
                     TQAHAIIKGIDFSAALKVPGVYKVFTGKDFPFKFGLYMKDRYVFAQDKVRFVGEQVAA
                     VIARTPQAAKQGAKLVKVEYEPLPVVLDQMQALEEGAELLHPDLDSYPHVPWFFPKGG
                     TNIAHWRKTRKGDLDKGFSEAEHILEDTYRVPRYAHCAIETHVAIGMLDHGGRLTVWS
                     SSQSPHTQRHLFAEALGMSHKDVRVIAPHVGGGFGGKAGVTMEIIPAALATRLKGYAV
                     KLRYTREEEFINTYQRLGVVAKIRMGVDKEGRITALDHKIYWDAGAYVEYGANVVNAV
                     GLSAIGPYKIDNVSIDSVCVYTNLPPGGAYRGFGYSEMLFGLESHVTRMAHQIGMDEI
                     EFRRLNAIRENDLTAYGAHMNPNGLIECIDKVKDAIRWGDKPLPTRPHTRIGQGFSIF
                     WKAPAMPPNASSSSFLKFNEDASINLIISGMDIGQGYQTVMAQIAAEILSVPPHRIRV
                     ETPDTDRNPYEWQTVASHITWSSGNSVKAAALDAREQILDLITRVYHYPKDELYLEDE
                     AVKCHTQPNFILPLRDFVVNGIMTADGTFKGGPINARGMYMPEFSSTNGDPETSQGGH
                     PNVHYTVGAAALNLEIDEETGKMKVLKAALAIDAGKALNPDLVRGQITGGLLQGLATV
                     LYEDMRYDASGKLLNPNFTDYKIPTSMDVPDEIIPIIVEVPQPDGPFGARGIGEHTML
                     PAAPMIANAVEDALGVRIKSMPVTAEKVAMSLFGEDK"
     gene            10510..11700
                     /locus_tag="PLO35_03645"
     CDS             10510..11700
                     /locus_tag="PLO35_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOH78790.1"
                     /translation="MSPALTGIRVLDLTRVLAGPFCTMVLSDLGAEVVKVEIPGTGDD
                     SRAFGPFLHNRSLYFLSINRAKKSVSLNLKHPRGKELFKRLVPQFDVLVENYRPGTME
                     KLGLDYELLKELNPRLIYAASTGFGHSGPDSLKPAYDILAQARGGIMSITGQDPNHPT
                     RVGMSLGDITAALFTAIGINAALYQRQITGRGQKIDVSMLDSQVAILENALVRYQAEG
                     VSPQPLGTRHPTIAPFQAYRARDRYFVIAVGNDALWKTFCSALERLELTDDPRFSSNR
                     LRTDNLSQLNAILEPVFLTKDASDWLALFELTGIPSAPINDIEAVINDPQVLSRNMIV
                     SVEDELAGTVKIAGNPIKMSTLEESPTRDRVPEIGEHNQLIYGGWLGLSDAELTALKA
                     DGAI"
     gene            11900..13207
                     /locus_tag="PLO35_03650"
     CDS             11900..13207
                     /locus_tag="PLO35_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOH78791.1"
                     /translation="MDIESILRGPIKSQVQMLQSGEIPLDLWLSTLVERLPKLDQTVR
                     SFLKEPGRSERVFSQIDELPARWPHPSSRPPLYGIPFGVKDIFAVDGLPTRAGSSLPP
                     SALRMPQSSLVTRLVNAGAVVLGKTVSTEFAFYSPGPTRNPKHLDHSPGGSSSGSAAA
                     VSAAICPFALGTQTIGSIIRPASYCGVIGFKPSYGRVTLEGVFPFSPLMDHPGIICNR
                     LDDLIYLAPIFLADMKPLLVKDKPRLGIPHPKYLAKADAAVLDNYQNRISILKSQGFQ
                     VTQTDLFENIDHLEKAHHDICSWQFYETHAKLWHRYADRYSPQSVAYMQRGKLLGPGM
                     FSSLQDDLLLQRAAITKRLDNLGIDLIIAPSTTTAAPEGLDSTGSPLMSLPFSYYGLP
                     SITLPDGQDAKGLPLGLQIGAAPGRDEYLLIASQDLGDSIGSP"
     gene            complement(13248..14360)
                     /locus_tag="PLO35_03655"
     CDS             complement(13248..14360)
                     /locus_tag="PLO35_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78792.1"
                     /translation="MNRALAILIMLFLLVMTGCDLFNSDGEEGWVSLAGYDFRVYMGH
                     FDDIESTDEEGNYLGDMVLGGNGDTHDWEMRDYPWLIRPGAKIKKPRDAKSGFNVTAH
                     NPLGDELNAEIFRDGVSTGFFTPHLFVDSEFLLGVYTVSLYGIAVWDPDQYVVVDNSY
                     DQSVDFVAPLITDLSSFTVTLTANNFVKIAWISQSETEMLGYRIYRGESSDVASAVLI
                     TPVMIPATNTSSPQSYSITDNEVAIGSTYCYWLEAVNYASSAFYGPVSITVVGEVPPE
                     IPIVNGIYRAYPNPVSQGGSFNLHIAIKEHITATLLLLDSQAEVKKEYSLAPGVVNHL
                     TVPTQDLTPGLYRVFLYTSDRQYAYGDVLVQAPRVP"
     gene            14519..14725
                     /locus_tag="PLO35_03660"
     CDS             14519..14725
                     /locus_tag="PLO35_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78793.1"
                     /translation="MLLANDLDAAREAAHQITAAYVIADFSKAAEELILGEMQASNPP
                     GVSLSRSVVVPSVLPVGPSLPEFV"
     gene            complement(15757..16368)
                     /gene="wrbA"
                     /locus_tag="PLO35_03665"
     CDS             complement(15757..16368)
                     /gene="wrbA"
                     /locus_tag="PLO35_03665"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOH78794.1"
                     /translation="MKTLILIHSLWGHIYRMAQAVAEGAKEIEGMDVEIKRVPETLSP
                     DIIAMMGATEAQKAMEHIPVANLDDLEEADGIIFGTGTRFGNMSAQMKAFLDSTGGLW
                     AKGALVGKVGSVFTSSGTQHGGQESTLLTFHPVLLHHGMVIAGLPYAFGGQTTMSEIS
                     GGSPYGASTIAGGDGSRQPSQNELDGARFQGKYVAGIVKKLAQ"
     gene            16677..>18958
                     /locus_tag="PLO35_03670"
     CDS             16677..>18958
                     /locus_tag="PLO35_03670"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOH78795.1"
                     /translation="MPSLPLVWWLAPLGAVSALFFAWWFWRSVHRENPGNARMVEIAG
                     YVREGAYAYLRQQYKGVGIFFAVALTLLLFMAYGLRVLDPMIPWGFLSGGFMSALAGF
                     IGMNTATMASSRTAQACSESLNLGLKVAFRAGGVMGLVVVGLALFDLSAWFFILNSIG
                     YSLQKIAVVMLSFGMGASSQALFARLGGGIYTKAADVGADLVGKVEADIPEDDPRNPA
                     TIADNVGDNVGDVAGMGADLYESYAGSILATAALGMSAFLQLSAKHPQVQASLFNFIA
                     APMILAGIGALLSIVGIFLVSVKEDAGMKQLMGALNRSVYVSSILIAVASYFITRYLL
                     PPQYFFGVFISTLVGLAAGVLIGYFTEIYTSHSYSHTKSIAQQGDYGSATVILEGISV
                     GMKSTMAPVIVIAIGIMTAFTVSNGFEIVEMGLYGIGFGAVGMLATLGVTLAMDAFGP
                     IADNAGGNAQMSYLPKEVRERTDNLDAVGNTTAAIGKGFAIGSAALTAMALLAAFLEK
                     VRDAFAMMGARLGETQFLTINYGSFSEKVDASKASLINFIEYYQINPLNPKFLLGIFV
                     GAMVVFVFSALTIKAVGRAAGKMVKEVRRQFTTIPGILQGTAKPDYARCVVISTQGAQ
                     QEMVLPAILGILTPIVIGLIFGVAGVLGVLIGGLTTGFVLAVMMNNAGGAWDNAKKYV
                     ETGAHGGKSSPTHKATIVGDTVGDPFKDTAGPCINILIKLMSMVSIVFAGLIVSYSPM
                     IEALYTPKPSGVTVIRSEMPA"
BASE COUNT         4175 a         5634 c         5086 g         4063 t
ORIGIN      
        1 agctctcaca gcgtagctct cacctcttcc gcatattatt cacatagaaa tacatcagcg
       61 cttccagttc cggttcgaag ctcagcagat caaacgcttg cggcagtctg gccgcctctt
      121 cactggcggc ctggagagcg gcccggtata gatcacaggc atcttccgga ccctgatgat
      181 ggtcctgaac tccggcaaag gcggcctcga tatccaaagc cagggccggg aggatatggt
      241 tggcatgatg ggtccactct ccgctgtgga tgtcaaactc atagacagac agaaacttat
      301 agccgtgctc gaccacaaac ttgacggcgt cgatgaggta atccacctcg cattcttcca
      361 tggcgtaatg gagattcagc ctcacccagc caggcttgat gccgctgtag cgcgcgttca
      421 cgatcaggca gcgatagtag tccgacacct gttgcggaat gcccatcaga taatggccat
      481 agggtcccgc gcaggaacac cccgcccggg tttggatgcc aaagaggtca ttgagcaggc
      541 gggtgacgaa cttgggatgc aggatgcggt cctggtggcg gatgttgaag ggcacgatcg
      601 gcaccttttt atccgcgtcc agcactccca gaaaagtgat ccgcgcgtca ccctcaaacg
      661 cgctcataaa cttctgataa taatagagct ccaggtcttc gatcacatca tggcccactt
      721 tgtctttgag ctggaaggcc agcgccaccc tgagggcttg caggatgcct ggagtgcctg
      781 gtttttcgcg ggtttcgatg tcgtggacgt attcttcctt gctgggagac acataatcca
      841 ccgttccccc tcccggcacg gttggcggca aggctgaggg gtagatatcg gcattgaaga
      901 tcagcactcc gctggtgcct ggtccgccca aaaacttgtg gggggagagg aagatggcgt
      961 cgaaatagct ctccgcgtca cggttcatgt cgattttcac atagggagcg caggccgcga
     1021 agtcgaagca ggccaggcct ccatgccggt gcaggattct ggccacctca tagacggggg
     1081 tcttgagacc gctcacgttc gaggccgcgg aaaatgatcc gatcttgagg cgattggcat
     1141 attcaggatc gctcaccagc tcttccagcc tctccagatc gagctccgcg tgcttgttca
     1201 tcggcacttc cacgatctcg cagagggtct ggcgccagag gatttcgttg gagtggtgct
     1261 cataggggcc cacaaacacg atgggcttgt tgtgatgcat aaagtcgtgc agggcctggt
     1321 tgcagtcgat gcccctggga ttgcggatta ggcagctttt gaggatctga cccactcttt
     1381 cccgggtggc gggtggccag taaaccccca ggatttgcag gagcttgacg attccacccg
     1441 tggtgccgga ctccgtgaag atgatcttgc ctttggggcc agcgttgacg gcatccttga
     1501 tagtatgctc cgcttcgtgc agcaggcggg tcatggtctt gccggtaaaa tcatcctcag
     1561 tgtgggtgtt ggcatagaat ctcaggacct tgccaatgta gttctcgatc gagatcaggc
     1621 ctctgccgct ggcagtgaaa tcagcgtaaa cgagaggacg ctctccatag ggagtgggga
     1681 tcggcatatt tctgccgata atgtcgcgcc gcagccaagc cagcccggcg atctgctttt
     1741 gggcggtgaa cttatccata atgccaaaaa atgcaagggg cgatatttgg gcaagaaaat
     1801 agttgcatcc agccaaaaaa cttgccggaa gggtgatcag tccttgccgg aagaccctgg
     1861 agggatagat tgtatctatg aggattccag caggacttgc catactcagc cacggggagg
     1921 acatccttcc cgctgtggat tggagcgttt acaagcgctt tgacagagtg gtgaattata
     1981 tccacaaaga ccgcctgctc agcgtttgcg ccgcttctat accctcagga gtggatcatc
     2041 tggtgctcaa cgtggatgac ctggaatggc tgcaaagcgt tcagaccctt acagataggg
     2101 ttgttctgaa cggtgtggcg ttccctctgg atccctgcaa gctttacaga gtgcctcccc
     2161 tgcctcttcc ggccagcttt gatcatatct taaccctgga gagctggtta cgcgcgcaag
     2221 ctccacccct cagcattccc agattgctgc atgaagctta ccggcccggc agctttgacg
     2281 ctgcccttgg cagggtcttt gaggagggga tcgaccttct ggaaagcagg gagtttctcc
     2341 aggccacgcg gttgctgaaa ggctgcggct ttggcagcac tccctccgga aacgatttta
     2401 tgagcggata tctgatggcg atagcctggt taacccaagc ggagtggcaa acgactggtt
     2461 tgcctgagtc aggaccatcc gtcggtaaac ggggacgttt accgctccac agagggagcc
     2521 aaaaaaatag gttgtcagaa attgccgatc tgattttgta tgaaatcctg ggaactgacc
     2581 cgctctgcga tgcttttgcc agacgcgcca gggccctgaa gcccgattcc gactggaaag
     2641 agctgctgca ggtctttgca gaccaatcag cggatgcccg gccagctctg gaagcggttt
     2701 tatcccatgg atccacgtcc ggagccgatg ctctggccgg cttctttatg ggactgcgtt
     2761 cctaccatat ggaggtgaaa tgattgtaaa agcgatggtg atccagggcc gctacctgga
     2821 ttcggtgaag ctgatgctga tctcgcgatc actgagctcc ctgccaggag ttcaggacgc
     2881 cgtggcgatc ctggccacac aggaaaaccg cggcatctta aaagccaccg atatgctggt
     2941 ggatgaggtg ctccacgcag ccgaaacgga tattgtcgtg gtggtcaagg ccagggacga
     3001 tgatagcgcc ttagccgcta tccaggaggc cagcaagacc atctcggacc caagccccac
     3061 aaatggctcc agggtccggc ttgccctggg gatggacgag gcccttaaac aagggagcga
     3121 cctctgcctg atctcgatcg cgggacgcta tgccgcccgg gaagctcgca aagcactcca
     3181 ggccggcctc catgtgatga ttttctctga taacgtgagc ctggaggacg agctcagcct
     3241 caagcggctg gccctctcca aaggtctcct gatgatgggc cccgattgtg gaacggccat
     3301 catcaacgga gtcccgctgg cctttgccaa tgccgttccc aaaggcggga tcgggatagt
     3361 ttccgcatcc ggaacgggct tgcaggagat cagcgctggg atcgccaacc ggggcatggg
     3421 gatcactcag gcctttggga ccggtggcag agacggcaag gaggccatcg gaggcctgat
     3481 gctgaaagcc tgcctcaaat atctgatcgc cgacccggcc acccaggtga tcgtcctcat
     3541 cgccaagacc cctcacccag aggtccgcaa agagctctgg gaactgattg gcggcacttc
     3601 caaaccggtg atcgtcaact ttatggacca catcgagcat cctcacctcg ataacctcca
     3661 ctatgccgtt tccctgcaga cctgcgccca tctggcctgt ttccattacg cccgcctcca
     3721 tggcctcaaa tatcaggaaa cccatgaggc gcttcccctt catcccacta ttcaaagtga
     3781 taaaagacgc tggctgagag gactttacag cggcgggacc ctctgcgcgg aagcccgtct
     3841 gatctataaa cacgagctgg gcggcgagcc ctacagcaac atcacaaacc gggcggacag
     3901 acagctcaaa gacccctggc gctcccttga agacagcctt atagacctcg gttcggatga
     3961 atacaccgtg ggacgtcccc accccatgat cgatctcagc ctgcgcatca aacgcctggc
     4021 cgccgaagcc agcgatcccg aagtggccgt catcctcctc gacgtggtgc tgggctatgg
     4081 cgcgcatccc gatcccgcct cagaactggc tcccgccata gcgaccgtcg ataaggatat
     4141 cctcatcgtc tgcagcgtct taggcacggc cggcgaccct caggaccggg cttctcagat
     4201 gtccatgctt cgggaagcgg gtgccgtggt cttcagttcc catcacgccg cggccgatta
     4261 tgccattcaa gctatcaaag ccataagggg gatttcatga gccttttcaa actgccggac
     4321 ggcaagcccc gcgtcctcaa cctcggtctg gaagcctttg cgatagactt ggaaagccag
     4381 ggagtggaag tcacccgcgt gagctggaaa ccacccatgg aactggcccc cggagccctt
     4441 gacaagctgc tcgcggcccg ggaaacgatc gatgcagcca atgccaaagc cctcgaaatc
     4501 atccagaatg gcaaaccgct gctggtgggc atgggcatcg ccagagaatt gatcccaggt
     4561 atgcacgacg accttatcct ccatgccggg cctcccatca cctgggacag gatgtgcggt
     4621 cccatgcggg gtgccgtgat cggggccctc atctatgaag gccgcgccaa aacggcttcc
     4681 gaagcggaag cgctggcctc ctcgggacgg atcaactacg cgccctgtca tgaacattct
     4741 gccgtgggtc ccatggcagg aatcatcagc gcctccatgc ccgtcttcgt gatcaaaaac
     4801 gagcgctacg gcaacctggc ctacgccacc cagaatgagg gtttgggcaa ggtattgcgc
     4861 tatggagctt tctctgccga ggtgatcgag cggctcaagt ggatggaaag cgtgctctat
     4921 cccactctca aacgcgctgt cgagcatctg ggccagatcg atctgatgag cctcattgcc
     4981 caggccctcc acatggggga cgaggtccac aaccgcaacc gggccggtac ttctctgctg
     5041 atccgtcagc tcgctcccgc gctgttgaaa accatgacca acgtggaaaa caccgcccgg
     5101 gtgctggatt ttatcaacgg caacgatcac ttcttcctca atctctccat gccggccgcc
     5161 aagtgcatcc ttgatcccgc catgaatatc ccaaatagct ctgtctgcgt caccatggcc
     5221 cgcaacggca ccgattttgg catccgggtg agcgggctgg gaaccagctg gttcacaggt
     5281 ccggctttgg tgcctgacgc cctttacttt cctggatata caaaagcgga cgccaatccg
     5341 gatattggag acagcgccat caccgaaacg ggaggctggg gaggttttgc cattgccgcg
     5401 gcccccgcga tcgtgcaatt tgttggcgga accgctgctg atgccttatc ttatactaac
     5461 accatgtatg agatctgcgc tggcgagagc acccgttacc agatcccggc cctggatttc
     5521 agaggcaccc cattggggat agacgttctc aaagtggtgc tcaaaaacat cactcccttc
     5581 atcgataccg gcgtggccca caaggaccct ggcgtcggac aggtgggggc gggagtggtc
     5641 caggcccctc ccgaaccgtt catgcgggcc tgcgaggcct tcgccgatct tatggaccgg
     5701 caaaggtcct cgtttgcctc ttctactgag acataactaa aagaatatta aggagataca
     5761 acccatgcag atggatcagt ttctggactt catgcaaggc ctgaagatgt atgacctcac
     5821 tcagcgcctc agcatccata cacctccctg gccaagctat atgccgctgg gaattcaata
     5881 ctttaaacgt atcgccggag cccatatggg ccagggcgcc aatggccaga ttatcaccac
     5941 cagcaatcac gtgggcaccc acatcgacgg cgaaatccac tttcacgcca gtggacgcac
     6001 cattggcgaa gtgcctatgc acgaatgggt tggacccggc gtcgtggtgg atatctcaga
     6061 cagcgtgggg gattacgacc tctacagccc cgagctgctg atgaagaaag cggacatccg
     6121 ctccggcgac atcctgatca tcaacaccgg ctatcataaa tacgcctggg accagccgga
     6181 atcagacgaa ctgcgctatt tcgtcaaaca tcccggaccg gaccgcaact tccataaatg
     6241 ggccctcgag atgaagatca agtggatcgg tgtggattgc ggatctgccg accaccccat
     6301 gaacaccatc atccgcacct ggcatcccaa gagctttatc gaagccgaaa acaagctcaa
     6361 agccacctac ggcatgagct gggacgagat gttccccacc gaagaatact accaggtgat
     6421 gcatctgaag ctcttcccca agaagctggt acacgctgaa aacctgggcg gcgacatcgc
     6481 tcaggtctcc aacaaacgcc tctggatcgg cctcttcccc ttgcgcggca tcgaactgga
     6541 atcgtccatg tgccgcgtca tcgccttcga accgtaacgc tggctttagc cggccgtagc
     6601 actgacttca gtcggtgcgt gcaagatact gctatccagt gaatacctct attacaactt
     6661 cagagagagc tgagaaagca gagaagtttt tacaatgaga tatgagaata tgagtttgta
     6721 aaacaatgag ttatgagaac atgtcaaaga agcaaggcaa agagcgcagc gcataacgca
     6781 taacgcataa cgcgtaacgc ttcacgcccc caggagacca tcatggcaat agcccccacc
     6841 tttgaatacg cccggccccg caacctggaa gaagccctgc gcctcaagga ccaataccgc
     6901 gaacgcgctt tggtcctggc cggaggcaca gatctgatcg ccaatctgcg ggaggatatg
     6961 atccacccgg aactgctgat cgacatcaag gatatccccg gtctggacaa aatcgtgatc
     7021 gaagcaggca ctctcaagct cggtccgctg gtcactttca cccagctcat cgagtttgga
     7081 ccactcggca aatcctttcc catgctgctg gacgcctccc gcaccgtagc ctcggtggga
     7141 atccgcaacc gcgccactct ggtgggcaat atctgctccg cggttccctc tctggacagc
     7201 gctccgcctc tcttgtgcca cgaagccgtg gtccactgcc tcagtctgga aggagagcgt
     7261 cacatccgga tcgacgactg gttcaccggt ccccgcaaaa cagctctcaa accggatgaa
     7321 atcgtcacgg gaatcactct gcaaccagtc ttccactccg gctccggcat ctatctcaag
     7381 ctgggccgct atggcggtga agacctcgcc caggccggtt ggggtatcct catagatggc
     7441 gcaaaccatt atcgcgtagc tcattgcgcc ctgggtccgg ttcccgcccg cgcctccaag
     7501 attgaagccc tgctggacgg caatcagctc aatgaagcgc tcattcagca ggctatggac
     7561 ctggttcctg aggagatttc ccccatcacg gacatccgtt ccagcaagga ataccgcacc
     7621 cacatcagta aagtgatcct caagcgtggt ctgctggcgg cgcgtgaacg cctggccggc
     7681 aaagacatcg acccccgtac ccttttggga ggcatataat gaaagaaatc catctgaccc
     7741 tcaatcacga agagcgcagc gcctgggtgg aaaccaacga aaccctcctg gaagtgctgc
     7801 gagacaaact gggagtcaag agccccaaat gcggctgcga acgtggtgac tgcggtgcct
     7861 gtacagtcct gatggatggc aaggcgatcc gcagctgtct ggtgttggcc gttgaagcgg
     7921 aagggcacag catcctcact gtagaaggcc tctcgcccga taagctcact gccctgcagg
     7981 agacctttat cgagttcaac agcttccagt gcggtttctg cgcccccggc atcatcctcg
     8041 ccatcaccga actgctggag tcccatccca agccgactct ggaagagatc aaggaatcca
     8101 tcgccggaaa cctttgccgc tgcaccggtt acactcccat ctttgatgcc gtaatggccc
     8161 atatccagag ggaggacaaa tgaaggactt caagtacatc ggcaaagacg tagtccgcat
     8221 cgatgcccgg gaaaagatca gcggagcggc tatctacggc gacgacatcg acttcggccc
     8281 caagctgctc tacgccgaga tcgtagccag cacccaggcc cacgccatca tcaagggcat
     8341 cgatttcagc gccgctctca aagtgccggg agtatataag gttttcaccg gcaaggattt
     8401 ccccttcaag tttggcctct atatgaaaga ccgctacgtc ttcgcccagg ataaagtgcg
     8461 ctttgtgggt gagcaggtag cggctgtgat tgcccgcacc cctcaagccg ccaagcaagg
     8521 cgctaaactc gtaaaagtgg aatacgagcc cctgccagtc gtgctggacc aaatgcaggc
     8581 cctcgaggaa ggagcggaac tgctgcatcc cgatctggac agctatcccc atgtgccctg
     8641 gttctttccc aaagggggca ccaacatcgc gcattggcgc aagacccgta aaggcgatct
     8701 ggacaaaggt ttcagcgagg cggagcacat cctggaagac acctacaggg taccgcgcta
     8761 tgcccactgc gccatcgaaa cccatgtggc catcggcatg ctggaccatg gcggccgcct
     8821 caccgtctgg agttcatccc aatcccccca cacccagcgt cacctctttg ccgaagctct
     8881 tggcatgagc cacaaggacg tccgcgtgat cgcaccccac gtgggaggag gctttggagg
     8941 caaggccggc gtgaccatgg aaatcatccc cgcggccctg gccaccaggc tcaaaggcta
     9001 tgccgtcaag cttcgctaca cccgtgaaga ggaatttatc aacacctacc agcgcctcgg
     9061 agtggtggcc aaaatcagga tgggagtgga taaagagggc cgcatcaccg ctctcgacca
     9121 caaaatctac tgggacgcgg gagcttatgt ggaatacggt gccaatgtgg tgaacgccgt
     9181 gggcctcagc gccatcggtc cttataagat cgacaacgtc tccatcgatt ccgtctgcgt
     9241 ctatacaaac ctccctcccg gaggagctta ccgcggtttt ggttattccg agatgctctt
     9301 tgggctggaa tcccacgtca cccgcatggc ccatcagatc ggcatggacg agatcgagtt
     9361 tcgccgcctc aacgccatcc gcgagaatga cttgacggct tatggcgctc acatgaaccc
     9421 caacggcctg atcgagtgta tagacaaagt gaaggacgcc atccgctggg gtgataaacc
     9481 cctgcccacc aggcctcaca ccagaatagg ccagggcttt tccatctttt ggaaggcccc
     9541 cgccatgcca cccaacgcgt cctcctcgtc cttcctcaag ttcaacgaag acgccagcat
     9601 caatctgatc atttccggca tggatatcgg ccagggctat caaaccgtca tggcccaaat
     9661 cgccgcggaa atcctcagcg tgccgcctca caggatcagg gtggaaactc ccgacacgga
     9721 ccgcaacccc tatgaatggc agacggttgc ctcccatatc acctggagca gcggcaacag
     9781 cgtcaaggcc gccgccctgg atgccagaga gcagattctg gacctcatca cccgcgtcta
     9841 tcactatccc aaggatgagc tctatctgga ggacgaagcg gtgaaatgcc acacccagcc
     9901 aaactttatc ctgccgctca gggactttgt ggtaaatggc atcatgaccg ccgacggcac
     9961 cttcaaaggc ggccccatca acgcccgcgg catgtatatg ccggagtttt cttccactaa
    10021 cggagatccc gaaaccagcc agggcggcca tcccaacgtc cattacaccg tcggagcggc
    10081 ggccttgaac ctcgagatcg atgaagagac tggtaagatg aaagtgctca aagccgcgct
    10141 ggccatcgat gccggcaaag ccctcaaccc cgatctggta agaggccaga tcaccggagg
    10201 cctcttgcag ggcctggcca ccgtcctcta tgaggatatg cgctacgacg cttccggcaa
    10261 actgctcaat cccaatttca ccgattacaa aatccccacc tccatggacg ttccggacga
    10321 gatcatcccc atcatcgtag aggtgcctca gccagacgga ccgtttgggg ccagaggcat
    10381 tggagaacac accatgcttc cggccgcgcc gatgatcgcc aacgccgtgg aagacgctct
    10441 gggcgtgcgg atcaaatcca tgcccgtcac agccgagaaa gtggccatga gcttgtttgg
    10501 ggaggataaa tgagcccagc cctcacaggc atccgcgtcc tggaccttac ccgtgtactg
    10561 gccgggccct tttgcaccat ggtcctcagc gatctggggg ccgaagtggt aaaggtggaa
    10621 attcccggca cgggtgacga ttccagggct tttgggccct ttctccacaa ccgttccctc
    10681 tactttctca gcatcaaccg cgccaaaaag agcgtctccc tcaacctcaa acatccccgt
    10741 ggcaaagaac tcttcaaacg gctggtgccc cagttcgatg tcctggtgga aaactaccgt
    10801 cccggcacta tggaaaagct gggcctggat tacgagctgc tcaaagagct caatccccgc
    10861 ctcatctatg ccgcctccac aggctttgga cacagcgggc cagacagcct caaacccgct
    10921 tacgacatcc tggcccaggc cagaggcggg atcatgagca tcaccggtca ggaccccaat
    10981 catcccaccc gggtgggtat gagcctggga gacatcaccg ccgcgctctt caccgccatt
    11041 ggcatcaacg ccgcgctata tcaacgtcag atcaccggac ggggccaaaa gatcgacgtc
    11101 tccatgctgg acagccaggt ggccatcctc gaaaacgcgc tggtccgcta tcaagcggaa
    11161 ggcgtttcac ctcaaccact tggcacccgc catcccacca tcgctccctt ccaggcctac
    11221 cgggccagag accgctattt cgtgatcgcc gtgggcaacg acgccctctg gaaaaccttc
    11281 tgctccgctc tggaacgact cgagctgaca gacgatcccc gcttcagttc gaaccgcctc
    11341 aggacggaca acctcagcca gctgaatgcc atcctggagc ccgtcttcct taccaaagac
    11401 gcctccgact ggctggccct ctttgagctc accggcatcc cgtccgctcc catcaacgac
    11461 atcgaggccg tgatcaacga tccccaggtg ctaagccgca acatgatcgt gagcgtggag
    11521 gacgaactgg ccggcaccgt aaaaattgcc ggaaatccca tcaaaatgag cactttggag
    11581 gaatccccca cccgtgacag ggtgcctgag atcggtgaac acaaccagct gatctatggt
    11641 ggctggctgg ggctcagcga cgcggaactc actgccctga aagccgatgg agcgatctga
    11701 actcgttgac acattgagat aatgagcggt ttattgaaca atgagaaatg agaatatgag
    11761 gattaattga gaattgacaa cggagaatgc tctcactagt ggagccgcaa acgtcctcgt
    11821 ttgcgtggcc ggatcgtcta tgacaattcg gtgcgtgcct tgcttcacgc gtaacgcgta
    11881 acccctaaaa ggatacccca tggacataga aagcatcctg agaggtccca tcaagtctca
    11941 ggtccagatg ctacaaagcg gagaaatccc tctggacctc tggctgagta ccctggtaga
    12001 acgcctcccc aaactggatc aaaccgtgag gtccttcctc aaagaacccg gcaggagtga
    12061 acgcgtgttt agccagatcg atgagcttcc ggcccgctgg cctcacccct ccagccgtcc
    12121 tcccctttac ggcattccct ttggcgtcaa agacatcttt gccgtggacg ggcttcccac
    12181 ccgggccggt tcttccctgc cgcccagtgc tttacgcatg ccccagtcct ctctggtcac
    12241 caggctcgtc aatgccggcg cggtggtatt gggcaagact gttagcaccg agttcgcttt
    12301 ctacagccca ggccccaccc gcaatcccaa gcatctggac catagtccgg gaggctcctc
    12361 aagtggttcc gcggcagccg tttccgctgc catctgtcct ttcgctctgg gcactcagac
    12421 catcggttcc atcatccgcc ccgcttccta ctgcggcgtg atcggcttca aacccagcta
    12481 tggcagggta acgctggagg gagttttccc cttcagtccg cttatggacc atcccggcat
    12541 tatctgcaac cggctggatg acctcatcta tctggccccc atctttttag ccgacatgaa
    12601 accactcctg gtgaaggaca aaccccgtct gggtatccct catcccaaat acctggccaa
    12661 agccgatgca gccgtcctcg acaactacca aaacaggatc agtattctga aaagccaggg
    12721 cttccaggtg acccagactg acctctttga gaatatcgac cacctggaaa aggcccatca
    12781 cgacatctgc agctggcagt tttatgagac gcacgccaag ctctggcacc gctacgctga
    12841 cagatattcc cctcaatcag tggcttatat gcaaagaggc aagctgctgg ggccagggat
    12901 gtttagcagc ctgcaggatg atctgctcct ccaacgcgcc gccatcacaa agcgtctgga
    12961 caacctgggt attgatctca tcatcgcccc ttccacaact accgcggccc cagagggcct
    13021 ggacagcacc ggttctccgc tcatgagcct ccccttcagc tattacggcc tgccttccat
    13081 cacgcttccc gatggtcagg acgccaaagg actgccctta gggctgcaaa taggggccgc
    13141 tccgggacgg gacgagtatc tcctgatcgc cagccaggac ctgggggata gtatcggcag
    13201 tccttgattc ctccctggac ctttagaaac taatcagcga taacggacta tggtactctg
    13261 ggcgcctgga ccagcacgtc cccataggcg tattgccggt cgctggtgta caaaaacacc
    13321 ctgtagaggc ctggagtcag gtcctgagtg gggactgtca gatgattaac aactcctggt
    13381 gccaggctgt attccttctt gacctcagcc tgtgagtcga ggagcaaaag cgtggcggtg
    13441 atgtgctcct ttattgctat atgcagattg aagctaccgc cctgagagac cggattggga
    13501 tacgcgcggt aaataccatt cacgatgggt atttccggtg gtacttcccc caccacggtt
    13561 atgctcaccg ggccatagaa cgcgctgctg gcatagttca cagcctccag ccaataacaa
    13621 taggtgctgc cgatagccac ttcgttgtcc gtaatgctgt agctctgagg actgctggta
    13681 ttggtggcag ggatcatgac gggagtgatc agtacagcgg aggccacatc actgctctcg
    13741 ccacgataaa tccggtagcc cagcatctct gtctcggatt ggctaatcca ggcgattttt
    13801 acgaagttat tagccgtgag cgttaccgtg aaactactga gatcggttat cagcggcgcg
    13861 acgaaatcca cgctctggtc atagctgtta tccaccacca catattggtc ggggtcccaa
    13921 accgcgattc cgtataaaga gacggtgtaa actcccaata gaaactcgga atccacaaac
    13981 aggtggggcg tgaagaatcc cgtggagact ccatctctga aaatctcagc gttcagttcg
    14041 tctcccaggg gattatgggc cgtgacgttg aagccgcttt tagcatcccg aggttttttg
    14101 atcttggcgc cgggacggat aagccagggg taatcccgca tttcccagtc atgggtgtcg
    14161 ccattcccac ccaataccat atcgcccaga tagttaccct cttcatccgt ggattcgata
    14221 tcgtcaaagt ggcccatata gaccctgaag tcataacccg cgaggctcac ccacccctct
    14281 tctccatcag aattgaagag gtcgcatccc gtcatgacca gcaaaaacag catgatcaat
    14341 atggctaaag ctctattcat ctatcgctcc ctggcctcca gcgcgggcat gccaagcggc
    14401 tttggaccca gcctggcttg ctgcctgtaa accagttttc aagccagctc agcggcaagt
    14461 ttttgtcaac gattttcttt atctttaatc ctttacaatg atatcgacat aagcttctat
    14521 gctgctggcc aacgatctcg acgcggccag agaggcagcc catcaaatca ccgcggccta
    14581 tgttattgca gatttctcca aagcagcaga agagctgatc ctgggagaga tgcaggccag
    14641 taatccacct ggtgtatccc tgtcacggtc agttgttgtc ccgtcggttt tgccagttgg
    14701 tccttctctg cctgagtttg tttaaaatcc tggccctgat tgggtttctg tgctgtgtca
    14761 gactgtcgcc tgaaagtgaa tcccgtcttg ccagatcatc aagttgctca cttctttgcc
    14821 ggattcttga gttgaatgca tccttagctt tggggtccct gatgccggtc ctcagtgaga
    14881 tcatgctttc cccaatcgcc ttctccatcc cggctctggc atccagatac tctttaagga
    14941 gttttcgtgt cttcccttca tcaaagttct cgtccgtaag cgcgttcatg aaggcacgtc
    15001 tggaatcctg ggatttttgc agtttggctc ggtttcgctc ccgtgattcc aggaattggc
    15061 gtcgaagctc cagggtgtgc ttattcccca taccgtcccg ccagggaacg aaagtagtct
    15121 cgttttgttc gggaggtctg tcttcggtca accattgcac aatcaaaatg ccgattatgc
    15181 cggcattcat tgccatggac agcaccagtg ctatcagaat ccatcggcag tggcgaaggt
    15241 gttcaaatcg atcagttggt cgatcgtgcc gccggtattg taggccaggc tggccgtaat
    15301 cgtctccgcg gcgtcccttt caatggtcgg agtttggtct ggtccgtgct gttttcaccg
    15361 caagaactga ggctcagcaa catcattgag cttagcagaa gcagtgcaac catgtaaaat
    15421 ggagtcttgt tcatgatgat ctcctgtctt taataataac acattaacta caatcatcca
    15481 cccctcaata ccccggattt ttttccacca tcacatagtt cactgtctct gatatggtta
    15541 cgattaagac ctcttccctg caatcagcct ttatatcccc tgccggatat gccgggcctg
    15601 tctcacccct aacccacccc aaagcttggg ttaggggtgc gataagcgtg cgataagcgt
    15661 gggatagtga aggggaagga tgaagattat caattgtaaa aagtaaggag ccggaaatcc
    15721 ggctcccctt ttatgcctca gaacctcgat aatctgttac tgggctagtt tttttacgat
    15781 ccctgccaca tatttaccct ggaaacgggc cccgtccagt tcgttctggc tgggctggcg
    15841 tgatccgtcc cctccggcaa tagtggaagc tccgtaaggc gatccgccgc tgatttcact
    15901 catagtcgtt tgtccgccaa aagcataagg cagaccggcg atgaccatac cgtgatgcag
    15961 gagcacggga tggaaggtga gcagggtgga ttcctgaccg ccatgctggg tcccgctgga
    16021 agtgaacaca ctgcccactt tgcctaccag ggcccctttg gcccacaagc ctccggtgct
    16081 gtccaaaaag gctttcatct gagcgctcat gttgccaaag cgggttccgg tgccgaagat
    16141 gatgccgtcg gcttcctcaa ggtcatcgag gttggctacc ggtatgtgtt ccatagcttt
    16201 ctgggcttcc gtggcaccca tcatggcgat gatatccggg ctcagggttt ccggcacgcg
    16261 tttgatctcc acgtccattc cttctatctc tttggcccct tcggcaactg cctgggccat
    16321 gcggtatatg tgtccccaaa gggaatgtat caggataagg gttttcattg taagttctcc
    16381 gttaatggtt cgtttgcaga cataataatc gggattgcat accacgcaag tgggagtttc
    16441 tcaggcaaag ctgaagctgt gatatcccgg gcagaaggcg tctcttctac cccctcccaa
    16501 aaaaacaaat tgacaaatcg ccaaaggccg gctaaaattg cattacaaga taagtaattg
    16561 atcttataag cgattagctt gttaatcaga tacttgcggg ttaacggaga atagtttgat
    16621 ctgcgcggat gtgtccaatc cgtgagtaaa cacaaccttt ttacatgtga ggtaacatgc
    16681 cgagtctgcc gcttgtttgg tggttggcac ccctgggtgc cgtgtcagcg ttgttttttg
    16741 cctggtggtt ctggcgctca gtgcaccggg aaaatcctgg caatgccaga atggttgaga
    16801 tcgccggcta tgtgcgggaa ggggcctatg cctatctgag acagcaatat aaaggcgtgg
    16861 gaatcttttt cgcggtggcg ctaacgctac tgctctttat ggcctatgga ctcagggtgc
    16921 tggaccccat gatcccctgg ggctttttga gcggaggttt tatgagcgct ctggcgggat
    16981 tcatcggcat gaacactgcc accatggcct ccagccgcac tgctcaggcc tgttccgaaa
    17041 gcctcaacct gggcctcaag gtcgccttcc gggccggggg agtgatggga ctggtggtgg
    17101 tgggactggc cctctttgac ctcagcgcct ggtttttcat cctcaattcc atcggatatt
    17161 cgctccagaa gattgccgtg gtgatgctct cctttgggat gggggcctcc agccaggccc
    17221 tctttgcccg tctgggagga ggcatctata ccaaggccgc ggacgtggga gcagacctgg
    17281 tgggcaaggt ggaagcggat attccggaag acgatccccg caaccctgcc accattgccg
    17341 ataacgtcgg agacaacgtc ggagacgtgg ccgggatggg agccgacctc tacgaaagct
    17401 acgcgggctc gatcctggca acggcggctt tggggatgtc ggcctttctg cagttatctg
    17461 ccaaacaccc tcaggtgcag gcctcgctgt tcaacttcat cgccgctccc atgatcctgg
    17521 ctgggatcgg agcgctgctt tccatcgtgg gcattttcct ggtttcggta aaggaggatg
    17581 ccggcatgaa acaactgatg ggtgccctaa accgcagcgt ctatgtgagt tccattctta
    17641 ttgcggtagc ctcatacttc atcaccagat atcttttgcc gccccagtat ttctttgggg
    17701 tgttcatctc gaccctggtg ggactggcag cgggggtgct gattggctac tttaccgaaa
    17761 tttacacttc ccacagctac agccacacca aaagtattgc ccagcaaggt gattacgggt
    17821 ccgcgacggt gatcctggaa ggcatctccg tgggcatgaa atccaccatg gcacccgtga
    17881 tcgtgatcgc gatcgggatc atgaccgcct tcaccgtcag caacggcttt gagatcgtgg
    17941 agatggggct ctatggcatc ggcttcggag cggtgggtat gctggccact ctgggagtga
    18001 ctttggccat ggacgccttt ggtcccatcg cggacaacgc tggcggcaac gctcagatga
    18061 gctatctgcc caaggaagtg cgcgaacgca ccgacaatct ggatgccgtg ggcaacacca
    18121 ccgctgccat cggaaaaggc tttgccatcg gcagcgcggc cctcacggcc atggcccttt
    18181 tggcagcctt tctggaaaag gtgcgcgacg cctttgccat gatgggggcc cgcctgggtg
    18241 aaacccagtt tctcaccatc aactatggca gcttcagcga gaaggtggac gcctccaagg
    18301 ccagcctcat caacttcata gaatattatc agatcaaccc cttgaatccc aagttcctgc
    18361 tgggaatctt tgtgggagcg atggtcgtct tcgtgtttag cgcgctcacc atcaaagcgg
    18421 taggccgggc ggccggaaag atggtcaagg aagtgcggcg ccagttcacc accattccag
    18481 gcatcctgca agggaccgcc aagccggatt atgctcgctg cgtggttatc agcactcagg
    18541 gggcccagca ggaaatggtg ttgccagcca tcctcggcat cctgaccccc atcgtcatcg
    18601 gcctcatctt tggcgtggcg ggagtgctgg gcgtcctgat cggaggtctc accaccggtt
    18661 ttgtgctggc cgtgatgatg aacaacgctg gcggggcctg ggacaacgcc aaaaagtatg
    18721 tcgagaccgg tgcccatggc ggaaaatcct ctcccaccca caaggccacc atcgtaggcg
    18781 acacggtggg cgatcccttc aaggacaccg cggggccctg catcaacatc ctcatcaaac
    18841 tgatgagcat ggtctctatc gtctttgccg gattgatcgt gagctactca cctatgattg
    18901 aagcacttta cacccccaag cccagtggcg tgacggtgat caggagcgag atgcctgc
//
LOCUS       DAOSFN010000034        18859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2375_length_18859_cov_9.647681, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000034 DAOSFN010000000
VERSION     DAOSFN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 18859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18859
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_18859_cov_9.647681"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..91
                     /locus_tag="PLO35_03675"
     tRNA            4..91
                     /locus_tag="PLO35_03675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Leu,seq:cag)
     gene            178..690
                     /locus_tag="PLO35_03680"
     CDS             178..690
                     /locus_tag="PLO35_03680"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH78796.1"
                     /translation="MEDYRLLTDKLNLIPHPEGGFYRRNWLSLLSGDLKESTGKIVFP
                     SRSIGSSILYMLPAEMVSKWHRVKCDEMWHHYRGIPLKMYMLSAQKGLEELQLGSDID
                     KGQLPQLIVHRDTWFAAEPMESKGYALCGCTLWPSFTYSDFELAETAKLADDFPAHRD
                     LILRIQNHPQ"
     gene            706..2043
                     /locus_tag="PLO35_03685"
     CDS             706..2043
                     /locus_tag="PLO35_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOH78797.1"
                     /translation="MQKIFILDTNVLIHNPQSLFSFAEHRVVIPIVVIEEIDNFKKGV
                     DEKSRNARQIGRYLDSLRERGSLQDGVPTDHGGIIQVTVNKDVTDTASRLFFLDRNDN
                     LIIGTALYFKNKFPDHEVTLISKDANVRIKADSVGINAENYETDTIKFDEFYTGWETL
                     EVEPAVFDEIVARKYVANSFGDFYPHEFVRLIDKTDPSRARNLKYVKSSNTFYAPQSY
                     TGQEVFGVKARNYEQEMALDLLLDDSIRLVSLSGKAGTGKTLLAMAAGLQKVVEDSKY
                     SRLVVSRPISPLGKDLGYLPGSKAEKFNPWMQPIYDNMDILLSLHDDIHNDSPKGKKK
                     PSIFDFIDYGFLELEPLTYIRGRSLPDQFIIIDEAQNLTPHEMKTIITRAGENTKLVL
                     TGDPYQIDIPYLDSQSNGLSVAVEKLKGEDLVGHVTLEKGERSKLADLAAKYF"
     gene            2044..3939
                     /gene="alr"
                     /locus_tag="PLO35_03690"
     CDS             2044..3939
                     /gene="alr"
                     /locus_tag="PLO35_03690"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOH78798.1"
                     /translation="MMVNQIKDRSWVEIDLSAFRHNLRQLKSLLKPGQSFLQIVKADA
                     YGHGAFEIGRTALEEGASCLGVANAEEGKLLRIQGCKAPILILSPSLPEEAEEIISFG
                     LTPTISDPIFATLLEEVAARADQLVNVHLKLDSGMRRSGASLEAFPALCEHVSSMPHL
                     KIEGIFSHYAASESDPEFSQSQLDSFIRRVRPFKNQVRYLHIANSAALLNGLTKGSNL
                     IRLGILSYGTYTHPDQKSRIALKPVMTFRSHIAQIKELKTGEAIGYNLSWTASRDTIY
                     GIVPVGYADGYDFLLSNRGRALVNQSLCPVIGRVSMDMICIDLTDAPQARVGDGVTLL
                     GAGNDAIRAEALANSYQGSAYELLCQVGRRARRYFTEGGNISHSAPLARRDFVSSDFS
                     DSKLNQIIQSAISQRLQSEEIGELISREILRVFFFNRDQDIHYRQKFVHHIQLEHSDQ
                     PEYWQATTRLSFSKVLQNDYFLVACATSDEALKRYFKRKEVEYRWLMDDKFELIGAAF
                     QLTDVYVNDLKLDTLVRINNSCLEIRCQHPDLKQLVGQEVSFRINTRAYYPKASHQLS
                     VFITELTHGVKVRLDYPPELQNLEVVPVFSGQNRYPKVTRVPGSVTVSTQVQQWVFPL
                     SGVVFAF"
     gene            4009..5457
                     /locus_tag="PLO35_03695"
     CDS             4009..5457
                     /locus_tag="PLO35_03695"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOH78799.1"
                     /translation="MDLADVSPGRISYREGKAQRPGDVVYWMQQSQRVWRNPAFELSK
                     RLAVTKAKPLWVLFALMADAPEANSRHYRFMIQGLFECAQTLRELSIGMFLGVGAPAD
                     ILGKLQDKISILVMDKGYLNWQRDIRRDVKASLPAHEIYEVEADVHVPVEVTSSKEEY
                     AAATLRPKILKILPYDLVCADDCIDSSGLTHTSDLSIPGLINLAKIPSESELWKLTIE
                     AIQPDESVSPGHFEGGYRQAGKRLDLFLNDRIHGYSEARNHPGLRHFSDLSPYLHFGQ
                     ISAQEISQRALDHFDLSPDDMSALIKSKGAFNPAAKDLAGFLEELIVRRELSCNYCHF
                     NHAYDEFQSLPAWAKTTLYDHWADPRPRDYSLDRLENAETDDPYWNLAQREMLMTGKM
                     NNYMRMYWGKKLIEWLPDPELSFRLMLWLNNKYELDGRDPNAYAGVAWCFGKHDRPWQ
                     SRPVFGSVRYMNKAGLDRKFDMSLYAQMIEDL"
     gene            5459..7984
                     /locus_tag="PLO35_03700"
     CDS             5459..7984
                     /locus_tag="PLO35_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOH78800.1"
                     /translation="MIESPAPSGRFGKADYWVTPARWFLPVIIATAMLFAFSGLGAQT
                     NQPPKYVISGDYNYPPYEYLDENQQPAGYNVELSKAIGKILGIQFEIRLTKWSLARQG
                     LEKGDVDLLQGMALSAERARTMHFSIPHTQTWRSIFVANASKIKSLDDIINATLIMQQ
                     GDVSQEYLSVIGFKGQIEECPTQTDALRLLNRGLYDATIVNHVHGMYLMKKNSFTNIS
                     VLSDKIQTRDYCYASMDEELIKKINLALIEMGRTGQLKRLQDKWFSSYRPRLVAGSFF
                     DGQHLKWTLPILAILLSMLAYAIVLSRKMKKKNQELLVKVSLCRAQEEELKRERDLFR
                     FGPVVVYKLQIEPLQMLYVSSNVSQFGYSEDEVKTFQTGLMSIVHPDDLPELYSSLAN
                     QNKEAPQPTENRYRILTKSGDYVWVYDYSLYQINDNGHIISYGYLIDISLSKELEENL
                     IDEKLKAEEGSLAKSQFLAGVSHEIRNPLNGILGLINLLRDMKMSTELREIYDLIDAS
                     GHSLQRLINDILDLSRIESGQLQKMTSAFNPNYLLEDLVNTFARQKERPSVDIRLNIT
                     DELPEILMGDHVRTRQIITNLMHNAIKFTSEGWVQLNASIYSKTEDSIRILFSVQDTG
                     VGIAPEHHEHIFDLYYQDNASKGKGSGTGLGLAIVKRLIGLLGGVIWMESEVGKGSSF
                     YIILPYSLVSEPENNVPEPLEEAKPDTLPPMRVLIVESDPVSRRLLNNQLKAWGLETD
                     LADSGNAAIEKAARQHYDLLLIDMQLPDTDGAAAAKAIRASQTSAAVIPPIFIMSSSD
                     RKEDKAMCLEAGVNEFITKPIIFRDLHKMLEKLSDESQGENHA"
     gene            7977..9221
                     /locus_tag="PLO35_03705"
     CDS             7977..9221
                     /locus_tag="PLO35_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOH78801.1"
                     /translation="MRRGYLVTLVFLTALIALSANDLTIKRENSVLRSGPASYHKVLA
                     KLPVNTRVRELNRKDGWIEVMFKTQQGYIAPSSLNAKTLNNDPFAAIASSSANSRVAQ
                     HSVTAGVKGFAQYYDSSYGFAADQSFWELATAYETDLDEYSSFYTETYSSSAPMSFMG
                     GWSIPKNPKPDYFSESHEGFGLAVAAVIAKAGLYSNPLMNRYVNYVGQNLVLASDAGD
                     ISFRFFILDISQPNAYACPGGYIFITKGMLQTVANEAELAFALAHEIAHVTRFHGMIE
                     LKARENQIGAESLFAELDEARPDAFSEDAKAIEAELENDIMQMFESLIQGRLDEYELE
                     ADQLALIFMARSGYKPAAASALMSRLYGRGNESNNQHYRLESIATRVTGIDNELRKYR
                     DTGSHFLHSERLQRYKADAGLR"
     gene            9237..9857
                     /gene="udk"
                     /locus_tag="PLO35_03710"
     CDS             9237..9857
                     /gene="udk"
                     /locus_tag="PLO35_03710"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOH78802.1"
                     /translation="MKKAPAQLILIGGGTCSGKTTIARAIGKRIHDLKSVIISHDNYY
                     KDLSHLPPDAASRVNFDHPDAIDTKYLIRDVKQMLKGKAVNVPDYSFVHHQRGEGTLC
                     IANADVIILEGIFALYYPDLLELSDLKIFVDTDADIRLARRIQRDTLERGRDVESVLT
                     QYLETVKPSHQAFIDPSKKNADVIIPGDREFDKVLYMLNAYLKYTH"
     gene            10029..12044
                     /locus_tag="PLO35_03715"
     CDS             10029..12044
                     /locus_tag="PLO35_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH78803.1"
                     /translation="MFQFNSQVLTALKGEMIASALIANGISVFGHHHKDGSAQGIFCA
                     NGQCAKCTVIADGVAVKSCMTEVKQGMRVQSADGLPELPDSDYVSERPRIEHLVTDVL
                     IIGGGPSGLAAAIELGKRNVQTLLIDDKHILGGKLVLQTHKFFGSEEDSRAGTRGHDI
                     GQLLAAEVESLPSVEVWLKSTALFVFSDKKVGILKDGVYKIVSPQRILNAAGAREKFL
                     KFPGNHLARIYGAGAFQTLVNRDLIRPTNRLFIIGGGNVGLIAAYHALQAGIEVVGLA
                     EAMPVCGGYKVHADKLQRFGVPIYTSHSILCANGEETVESVTIAALDDKFKTINGTEK
                     SFKCDTILIAVGLDSLSEFTIEAEAAGIPVYAAGDAFEIAEASSAMFNGKIAGLKIAA
                     DSLAGQADVEAIPEEWYAKADLLKSHPGPVKGYQNHGAETGVFPIIHCLQEIPCNPCT
                     TVCPTNSIQTEDGGLMAVPLYKGSCIGCNKCVLICPGLAITLVDYRKDPEHPTVTIPY
                     EISNFKRQKGDLIDLVDIDGNDLGSYAIDTVLDFKDRHTQLVKVSVPKAIAKQVAAFR
                     VQDKQVSEPLAEAIIPQKLGDDAMICLCERVSVGQVRQLIRSGITDLNQIKAVTRAGM
                     GPCGAKTCETLIKGVLREEGIPTESVVPNTKRPVFIEVALDIFPDGV"
     gene            12046..13200
                     /locus_tag="PLO35_03720"
     CDS             12046..13200
                     /locus_tag="PLO35_03720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOH78804.1"
                     /translation="MNNTYDFIIIGAGSIGLPAALDLAEKGQKVLVIDPEHGPGQENN
                     KKAIGGVRATHSDYGKISVCQRSIDILKHWKQERGDDIGWMSNGYSYPAWEAKDADAL
                     LALMKVQHSFGLNIKWVSAEEYNELVPGINMENLIGSTYSPEDGSCSPLMLSSSIYFH
                     ALRKGVEFRFGEKVVSFSKREDRIISCKTDKDEYQAGGFVNAAGNYAREIGAMAGSDI
                     QVGPDNHEAGITEPVARFFGPMVIDMRKRPGSANFYFYQNHEGQVCFCYTPEPPILGV
                     DNRSTSEFLPHCSKRMLEVLPRLRYLKVRRTWRGQYPMTPDGFPIVGFKQECSNLLHA
                     VGMCGQGFMLGPGMGELISRICLKETSDSDARILKSFDPYRDFSGMEAFK"
     gene            complement(13579..15228)
                     /locus_tag="PLO35_03725"
     CDS             complement(13579..15228)
                     /locus_tag="PLO35_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78805.1"
                     /translation="MTHRILIAVTIALLLFSCTSNKVQTSSEPVPAFTGTVTKVAILP
                     LKAADSGSRNVVKIMSIRDLALAFNSHPKYSLLDMDVVAKQFKDSGFTNVDALEKEQL
                     MQMTKLTGSDVVIIGNVSESRPGLFAVNTRFYSSRSDELKQHTFNVVTNRDQRLAMMQ
                     EDFLPELDRFISNEVDKLLNIAVQNYVTQKYEDAVRGFNSVIGLNPDKGDAYYYLGAT
                     YYKQEKYTEAEQNLEKAITLNTEDQRAQLMLIEMYEITKQTDKRLKLMEAMAVKNPDE
                     ELWLAIGNLYDEAGNKAKAKESFQKSLEINPDYSQALIRLSFMLFEEGDYVGAIPQLE
                     KAADLFPDNQLVSDRLAASYFKANRLTEAITRYENILKSDPQNIQAYLTIAGLYRTQA
                     GESRDSKVTNELNTKAITALNQAKKIDPENAMVYLNLAAIYLSQKKNSEAESNANTAI
                     AKNPSLYQPYVILASVTQSKGTDQYNRFADLEKQAAKAVGKKANQLKKDRDAAKASAL
                     NFLRKAKEQLEAGRSRTTDSSALSDINSRISSINNMISQVQ"
     gene            complement(15459..16115)
                     /locus_tag="PLO35_03730"
     CDS             complement(15459..16115)
                     /locus_tag="PLO35_03730"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOH78806.1"
                     /translation="MEKQGMPLLGDKFPDMKVQTTRGEMELPKAMAGKWFVLFSHPAD
                     FTPVCTTEFVAFQNLYPKFRALNCELIGMSVDQVFSHIKWEEWIKENLKVDIQFPIIA
                     DTGAVASKLGLIHPGKGTNTVRAVFIVDDKAAIRIIFYYPQELGRNMEEILRAVEAMQ
                     FSDKNSCAMPANWPKNELVGDHIIIPPAKDVDSAQKRKEQAAKGEFECFDWWFCHKKI
                     "
     gene            complement(16350..17681)
                     /locus_tag="PLO35_03735"
     CDS             complement(16350..17681)
                     /locus_tag="PLO35_03735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOH78807.1"
                     /translation="MKIAVIGSGYVGLVSGTCFADMGNAVICVDKDPRKIDILNGGDI
                     PIYEPGLKEMVIANHQSGRLTFTTNLQSAVEASRIIFIAVGTPPDEDGSADLSHVIAA
                     AREIALYMNGSKIIVNKSTVPVGTADLVTRTVQSVLSERGVGHKFSVVSNPEFLKEGA
                     AIEDFMKPDRVILGVGDEETAAIMRELYKPFNRSADRVIVMAVRSAEMTKYAANALLA
                     TKISFMNELSRLCETLQADISEVRAGIGSDSRIGNKFIYPGVGYGGSCFPKDVKALIS
                     MHRENGSISSILDAVEDTNRRQKMVLVEKLISLWGDDMSGKTVALWGLAFKPQTDDMR
                     EAPAIVIARALIERGARVQAFDPVAMKVAAEVFRGLERIDLMPDEYTAVKDTDAMLLV
                     TEWHQFRYPDFERVKSLMKHPLILDGRNQYEPRSMKALGFDYYAIGRPRLS"
     gene            complement(17678..18619)
                     /locus_tag="PLO35_03740"
     CDS             complement(17678..18619)
                     /locus_tag="PLO35_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH78808.1"
                     /translation="MKILVTGGAGFIASHVTQAYLDQGHEVVVVDDLSTGYRHNLDPR
                     AKFYKLDIRDPQLAEIFASEQPDLVNHHAAQISVPLSIEDPQFDASVNVLGFINILQC
                     CVKYRVKKVIYISSGGAIYGEADEYPTTENYHPTPLSVYAINKMAGEHYLHLYHHQYG
                     LNYTVLRYANVYGPRQVSHGEAGVVSIFIEKLLQGLIPTVYAYPDEPEGMIRDYVFVA
                     DVVRANLLALHEGNNDRFNIGTCVETTTTQLYNTIQWKLGLDVPPHRGEARKGDLRRS
                     MLDYSKAYRVLGWSPIYNLEDGVKETIAYFKKQGAPS"
BASE COUNT         4647 a         4919 c         4862 g         4431 t
ORIGIN      
        1 cgtgctgaag tggtgaaatt ggtatacacg ctagtttcag ggactagtga gcgatgagct
       61 catgggggtt cgagtccccc cttcagcacc atttttttat ccacgaaaat aaaagcttga
      121 cgcctgatcg catctggtaa atccttgatc aaagatcaaa atgaaaaaga gcgtgttatg
      181 gaagactatc gcctgttgac ggacaagctt aatctcattc cccaccccga gggtggtttt
      241 taccgccgga attggctttc actgctaagt ggcgatctca aggaatccac tggaaagatc
      301 gttttcccct ctcgcagcat tggatcttcg atcctctata tgctccccgc tgaaatggtc
      361 agcaagtggc atcgggtcaa atgcgacgag atgtggcatc actacagggg tatcccgctc
      421 aagatgtaca tgctcagcgc tcagaagggg cttgaagagc tacaattggg ttccgatatc
      481 gacaaaggcc agctgcctca acttatcgtt cacagagata cctggttcgc tgcagaaccc
      541 atggaaagca aaggttacgc gctctgtggc tgcactttgt ggccctcctt cacctattca
      601 gacttcgaac tggccgagac cgccaaacta gctgatgatt ttccagctca cagagatctg
      661 attctccgca tccagaatca cccccaatag aaaaggtttt tagctatgca aaagatattt
      721 atcctggata caaacgtcct catccacaat ccccaatccc tgttttcctt cgcggaacac
      781 cgggttgtga tcccaatcgt ggtaatcgaa gagatcgaca acttcaagaa aggcgtggat
      841 gagaaaagcc gcaacgcgcg tcaaataggg cgctatctgg attcactgcg tgaacggggt
      901 tctctgcagg atggcgtccc cacggaccat ggcggcatca tccaggtaac tgtgaataaa
      961 gacgttactg atactgcctc ccgcttgttt ttcctcgatc gcaacgataa cctcatcatc
     1021 ggcacggccc tctacttcaa aaataagttt ccagatcatg aggtcactct gatctccaaa
     1081 gatgccaacg tccgcatcaa agcagacagc gtgggcatca atgctgagaa ttacgagacc
     1141 gacacgatca aattcgatga attctatacc ggttgggaga ccctggaagt ggagccagcc
     1201 gtatttgacg agatcgtggc caggaagtat gtggccaaca gctttggcga tttctatccc
     1261 catgagtttg tgcgattgat agataagaca gatccttcca gggcccgcaa cctcaaatat
     1321 gtaaagtcca gcaacacttt ttacgcgccg cagagctaca ccggccagga agtctttggc
     1381 gtcaaagccc gcaattatga gcaggaaatg gcgctcgacc ttctgctgga tgacagcatc
     1441 cgtctggtaa gcctttccgg caaagccggc acgggaaaaa ctcttctggc tatggcagcg
     1501 ggattgcaga aagtggtaga ggacagcaag tattccaggc tggtggtatc gcgtcccatc
     1561 tcccctctgg gcaaggattt gggttatctg cccggctcta aagcggagaa attcaacccc
     1621 tggatgcagc ctatctatga caacatggat atcctcctct ccctccacga cgacatccac
     1681 aacgactctc ccaaggggaa aaagaaacct tccatcttcg actttattga ctacggcttt
     1741 ttggagctgg aacccctcac ctacatcagg ggacgcagct tgccagacca gtttataatc
     1801 atcgacgagg ctcagaatct cactcctcat gagatgaaga ccatcatcac cagagcaggg
     1861 gaaaacacta aactggtcct gaccggcgat ccctatcaaa tcgacattcc ctatctggat
     1921 tctcaaagca atggtctgtc cgtcgcggta gaaaagctca aaggcgaaga cctggtaggc
     1981 catgtgacgc ttgaaaaggg agaacgctcc aagctggcag acctggcagc gaagtatttt
     2041 tagatgatgg taaaccagat caaggaccgt tcctgggtcg agatcgatct gagcgcgttc
     2101 aggcataatc tgcgacagct caaatctctg ctgaagccag gacagagctt tctgcaaatc
     2161 gtgaaagccg acgcctatgg ccacggggct ttcgagatcg gcaggaccgc tctggaagaa
     2221 ggtgcttcct gtcttggagt ggccaacgcg gaagagggca aactgctcag aatccagggt
     2281 tgcaaagcgc caatcctgat tctatctccc tccctaccgg aagaagcaga agaaatcatc
     2341 agttttggtc tcactcccac catctctgat cccattttcg ccacccttct ggaagaggtc
     2401 gcggcgcgcg cggatcaact cgtaaacgtc cacctcaagc tcgatagcgg catgagacgc
     2461 agtggggcca gtctggaagc ctttcctgcc ttgtgtgagc atgtaagctc gatgcctcac
     2521 ctgaagattg aaggcatctt ttcgcattac gctgccagcg aaagcgatcc tgaattcagc
     2581 caaagccagt tggatagctt catccgtcgc gtccggccct tcaaaaacca ggtcaggtat
     2641 ctgcacattg ccaatagcgc cgctctgcta aacgggctta ctaaaggctc caatttgatc
     2701 agacttggca tcctgagcta tgggacctac acccaccccg accaaaaatc cagaatagcc
     2761 ctgaaacccg tgatgacctt ccgctctcat atcgctcaaa tcaaggagct caaaaccgga
     2821 gaagcaatcg gttacaatct cagttggacc gcctcacgcg atacgattta tggcatagtg
     2881 cccgtgggtt acgccgatgg ctatgacttt ctgctttcca accggggccg ggctctggtg
     2941 aatcaaagcc tctgccctgt aattggcaga gtgagcatgg atatgatctg tatagacctg
     3001 acggatgctc cccaagccag agttggagac ggagtcacgc tgttgggagc gggaaatgat
     3061 gccatacgcg cggaagctct ggcaaactcc tatcaggggt cggcttatga gctgctctgc
     3121 caggtaggca gacgggcgcg ccggtacttt acagagggcg gcaatatctc gcacagcgct
     3181 cccctggcca gaagggattt cgtctcgagc gacttcagcg attccaagct caatcagatc
     3241 atccaatcgg ccatctcgca acgtctgcaa agcgaggaga tcggcgaatt gatctcgagg
     3301 gagatcctca gagtattctt tttcaaccgc gaccaggata tccattaccg ccagaagttc
     3361 gtccaccaca ttcaactcga gcattccgac cagcctgaat actggcaggc caccacaagg
     3421 ctgagcttct ccaaagtgtt gcaaaatgac tactttctgg tagcttgtgc cacatccgac
     3481 gaggctttga aacgttattt caaacgcaag gaagtggaat accgttggct gatggatgac
     3541 aagtttgaac tgatcggggc ggcttttcag ctcactgacg tgtatgtgaa cgacctcaag
     3601 ctcgacacac tggtcaggat caacaattcc tgccttgaga ttcgctgtca gcatccggat
     3661 ttgaagcagc tcgtaggcca ggaagtgagc tttcggatca acaccagggc ctattatccc
     3721 aaggcatcgc accagttaag cgtttttatt acggaactga cccatggagt caaagtcagg
     3781 ctggattatc cgcccgagct gcagaatctg gaagtagtgc cggtattttc aggccaaaac
     3841 cgctatccca aggtgacccg cgtgcctggc agcgttaccg tctccaccca ggtccagcaa
     3901 tgggtgtttc ctttatcggg agttgtgttt gcattttaaa cttagccatt ttgtaacagc
     3961 ttttatctcc attatgcggc atcgcctaaa ctggaaagag gccctgtagt ggatttagca
     4021 gacgtttccc caggcaggat atcatatcgg gagggcaaag ctcagcggcc gggggatgtc
     4081 gtttactgga tgcagcaaag ccagagagtg tggcgcaatc ctgcctttga gctctccaag
     4141 cgcttggccg tcaccaaggc caaaccactc tgggtccttt tcgcattaat ggctgatgcc
     4201 cccgaagcga atagccgcca ctatcgtttt atgattcagg gcctgtttga atgcgctcaa
     4261 acgctcaggg aattgagcat aggcatgttt ttgggggtcg gggctcctgc tgatatcctg
     4321 ggaaaactac aggacaagat atctatcctg gtgatggaca agggttatct gaactggcag
     4381 cgggatatca ggagagacgt gaaagcgtct ttacccgcgc acgagatcta tgaggtggaa
     4441 gcggatgtcc atgttcccgt ggaggttacc agttccaaag aagagtatgc cgctgccact
     4501 ctcaggccca aaatcctgaa aatcctgcca tatgatctgg tctgtgcaga cgattgtata
     4561 gactcttccg gattgacaca cacctctgat ctatccattc caggattgat aaacctggcc
     4621 aaaattccca gcgaatccga actctggaaa ctcacgattg aagccatcca acctgatgaa
     4681 agcgtgtcac cgggccattt tgaaggtggc tatcgccagg cgggcaagcg tctggacctg
     4741 tttctaaacg atcgtatcca cggttactca gaggcacgca atcatccagg cctgcggcac
     4801 ttcagcgatc tctctccata cttgcatttt ggccagatat cagctcagga gatcagccag
     4861 agagctttgg atcattttga cctgagccct gatgatatgt ctgccctgat caagtccaaa
     4921 ggtgctttca atccggccgc gaaagacctc gcgggctttt tggaggaact gattgtgagg
     4981 agggaactga gctgcaatta ctgccatttc aatcacgcct atgatgagtt tcagagtctc
     5041 cccgcctggg ccaaaaccac gctttacgat cattgggcgg atccccgccc gcgtgattat
     5101 tcacttgaca gattagagaa cgcggaaaca gatgacccat attggaattt ggctcaacgt
     5161 gaaatgctga tgacaggtaa gatgaataat tatatgcgta tgtactgggg aaagaagctc
     5221 atagagtggc ttcccgatcc ggaattgtca tttcggctga tgctgtggct gaacaacaaa
     5281 tacgagctgg acggtcgcga tcccaacgcc tatgccgggg tggcctggtg ctttggaaaa
     5341 cacgaccggc cctggcagag cagaccggtg tttggcagcg tgagatatat gaacaaagcc
     5401 ggcctcgata gaaagtttga tatgagtcta tacgcccaaa tgattgagga tttgtgagat
     5461 gattgaatcg cctgctcctt caggacgctt tggcaaagca gactactggg ttacccccgc
     5521 cagatggttc ttgccggtca taatcgcgac agcgatgctg tttgcttttt ctggattagg
     5581 agcacaaacg aatcagcctc ccaaatatgt gatcagcggc gactacaatt atcctcctta
     5641 tgagtatctg gacgaaaacc agcagcccgc gggttacaat gtggaactca gcaaggccat
     5701 cggtaaaatc ctcggcatcc agtttgaaat ccggctcacc aagtggtcgc tggcacgcca
     5761 gggcctggaa aaaggtgatg tagacctcct tcagggaatg gccctctcag cagaaagggc
     5821 caggaccatg catttctcca ttccgcatac ccagacctgg agaagtattt tcgtggccaa
     5881 cgcctcaaag atcaagtctc tggatgatat tatcaatgct actctgatca tgcagcaggg
     5941 agacgtatct caggaatacc tcagcgtgat cggtttcaag ggccagatcg aagaatgccc
     6001 aacccagaca gacgcgctca gacttctgaa ccggggcctg tatgacgcta ccatcgtcaa
     6061 tcatgtgcat ggcatgtacc tgatgaagaa gaacagcttt accaacatca gcgtcctctc
     6121 cgataagatc cagacccgtg actattgtta cgcttcgatg gatgaagagc tgatcaaaaa
     6181 gatcaatctg gcgctcatcg agatggggcg caccggacag ctcaaacgtc tgcaggacaa
     6241 atggttttcc agctatagac cgcggttggt ggccggctct tttttcgatg gccagcatct
     6301 caaatggacc ctgcctatcc tggcaattct gctctcaatg ctagcctacg ctatcgtcct
     6361 cagccgcaag atgaagaaaa agaatcagga actgctggtg aaagtaagtc tctgccgcgc
     6421 tcaggaagaa gagctgaaga gggagagaga cctgttcaga ttcggtccgg tggtagttta
     6481 caaactgcag atcgagcccc tccagatgct ctatgtatct tccaacgtca gtcagtttgg
     6541 ctactccgaa gatgaagtca aaaccttcca aacaggcctg atgagcatcg tgcatccaga
     6601 cgatctgcct gagctctatt cttctctggc caatcagaac aaggaagctc cccagcccac
     6661 tgaaaaccgc taccgcatcc tcaccaagag cggagactat gtgtgggtgt atgactactc
     6721 actgtaccag atcaacgata acggccacat tatctcatac ggttacttga tcgatataag
     6781 cctcagcaaa gaactggaag agaatctgat cgacgagaaa ctaaaagcag aggaaggcag
     6841 cctggcaaaa agtcagtttc tggccggtgt cagccatgaa atccgcaacc cgctcaatgg
     6901 tatcctgggt ttgatcaatc tattgcggga catgaaaatg agcaccgaac tgcgggagat
     6961 ttacgatctc attgacgctt ccggacacag cctgcaaaga cttataaacg acattcttga
     7021 cctctcgaga atagaatctg gccagcttca gaagatgaca agcgcgttca atcccaatta
     7081 tctgcttgaa gatctggtaa acacctttgc ccgtcagaaa gaaagaccca gcgtggatat
     7141 cagattgaac atcaccgacg aactgcctga aatcctgatg ggagaccacg tcagaacccg
     7201 ccagattatc accaatctca tgcacaacgc gatcaagttc accagcgaag gctgggtgca
     7261 gttaaatgcc agcatctaca gcaaaaccga ggactcgatc agaatcctgt tttctgtcca
     7321 ggacactgga gttggcatcg ctccagagca tcatgagcat atctttgatc tctattatca
     7381 ggacaatgcc agcaagggca aaggaagcgg aaccggtttg gggctcgcga tcgtgaagcg
     7441 gctgataggg ctcctgggtg gagtcatctg gatggaaagt gaagtgggca agggcagcag
     7501 tttttacatt atcctgccat actcgctggt ttctgagcca gaaaacaatg tccccgaacc
     7561 ccttgaagaa gcaaaacctg acacgctgcc ccccatgcga gttttgatcg tggagagtga
     7621 tcccgtgagc cgtaggcttt tgaacaacca gctaaaagct tggggtttgg agacagacct
     7681 tgccgattca ggcaatgccg ccattgaaaa agccgcgcgg cagcactatg atctcctcct
     7741 gatcgatatg cagttgccag atactgatgg cgcagccgcc gcgaaggcta tccgtgctag
     7801 tcaaacttcg gctgcagtca tcccccctat ctttattatg agttcatcag accggaagga
     7861 ggacaaggcc atgtgcctgg aggcgggtgt aaacgagttt atcacaaaac caataatttt
     7921 cagggacttg cacaagatgc tcgagaagct gagtgacgaa tcccaaggag agaatcatgc
     7981 gtagaggcta tcttgtcacc ttagtctttc taactgcact tatcgctctg agcgcgaatg
     8041 atttgaccat taagcgggaa aacagcgtcc tgcggtccgg tcccgcttct tatcataagg
     8101 tgctggccaa gctgcctgtc aataccaggg tcagggagct gaatcgcaaa gacggctgga
     8161 tcgaagtgat gttcaagacc cagcaaggct acatcgctcc cagctcgcta aacgccaaaa
     8221 ccctgaacaa cgatcccttt gccgcgattg ccagcagttc cgcaaactcg cgggttgccc
     8281 agcacagcgt tacagccgga gtgaaaggtt ttgctcaata ctacgacagc agctatggat
     8341 ttgccgcgga ccagagcttc tgggaattgg ctaccgcata tgaaactgac ctggatgaat
     8401 acagcagctt ttatacagaa acatactcca gttcggcacc catgagcttt atgggaggat
     8461 ggtcaatccc caaaaacccc aaaccggact acttcagtga atcgcatgaa ggtttcgggc
     8521 ttgccgtagc cgctgtaatc gccaaagcag gtctgtatag caacccgctg atgaacagat
     8581 atgtgaacta tgtaggccaa aaccttgtgc tggcatcaga tgccggagat atcagcttca
     8641 ggtttttcat cctcgatatc agccagccaa atgcctatgc ctgccctggc ggatatatct
     8701 tcatcaccaa agggatgctg caaacggtgg ccaacgaagc ggaactggcc tttgccctgg
     8761 ctcacgaaat tgctcacgtc actcgctttc acggcatgat cgaattgaaa gccagagaaa
     8821 accagattgg tgctgaatct ctcttcgccg agctggatga agcgcgtccc gatgccttta
     8881 gcgaagatgc caaagccatc gaagcggaac tcgagaacga cattatgcag atgtttgaat
     8941 ccctcattca gggtcgcctg gatgaatatg aactggaagc cgaccaactg gccttgatct
     9001 ttatggcacg ttccggctac aagcctgctg ccgcttcagc tttgatgagc agactgtatg
     9061 gccggggcaa cgaatccaac aatcagcatt accgactgga aagtatcgcc acccgcgtca
     9121 ctggaatcga caacgagctg cgcaaatatc gggatacggg gtctcatttt cttcactctg
     9181 aaagacttca gcgctataaa gcagacgccg gtttgaggta gctaagacag caggacatga
     9241 agaaggcccc cgcccaattg attcttatcg gaggtggcac ctgttcgggt aaaaccacca
     9301 ttgccagagc gataggcaaa cgcatccatg atctcaaatc ggtgatcatt tcgcacgaca
     9361 attattacaa ggacctcagt catttacctc cagatgccgc cagccgtgta aactttgacc
     9421 atcctgacgc gatcgacacc aaatacctga tcagggacgt gaagcagatg ctgaaaggca
     9481 aagccgtcaa cgttccggat tatagctttg tccaccacca gaggggtgag ggcactctct
     9541 gtatcgccaa tgccgacgtc atcatcctgg aggggatttt cgcgctctac tatcccgatc
     9601 tgctggagct ttccgatctc aagatcttcg tggataccga tgccgatatc agactggcca
     9661 gaaggattca gagagacact ctggagcgag gccgtgacgt ggaatccgtt ctgacacagt
     9721 atctcgagac cgtcaaaccc tctcatcaag ccttcatcga tcccagcaaa aagaacgccg
     9781 acgtgattat tcccggtgat cgtgaatttg acaaagtgct ctacatgcta aacgcttatc
     9841 tcaaatacac gcactaagag cagggcaaac agggcagcgg atattcacaa aaacagcggt
     9901 tttgcttgac accaggcccc cctctcactt tttgagcacc atacagcatc caaggagttt
     9961 ccaacagcat ggaaaatcgc agattagata gtcatcccat tcttcctgta cccccgcggg
    10021 aagagatctt gtttcagttc aattctcagg tcctgacggc cctcaaaggt gagatgatcg
    10081 cctccgcgct gatcgccaac gggatttcag tgtttgggca tcatcacaag gacgggagcg
    10141 cccaggggat tttttgtgcc aatgggcaat gtgccaaatg caccgtcata gctgatggtg
    10201 tggcagtaaa atcctgtatg acagaggtta aacagggaat gcgcgtccag agcgctgatg
    10261 gccttcctga gctgccggat tcggattacg tgtctgagcg tcccaggatc gagcaccttg
    10321 tgaccgacgt attgatcatt ggcggtggcc cttccggtct ggccgcggcg atcgaattgg
    10381 gcaaacgcaa cgtgcagact ctgctcatag acgataaaca tatcctgggc ggcaagctcg
    10441 tcttgcagac tcataagttt tttggctcgg aagaagacag ccgggcaggc accagaggcc
    10501 atgacattgg acaactgctg gccgcggaag tggagtctct tccctctgtg gaagtatggc
    10561 tcaagagcac tgccctgttt gtgtttagtg ataaaaaagt gggcatcctc aaagacggtg
    10621 tctataagat agtatcgccc cagcggattc tcaacgcggc cggggccaga gaaaaattcc
    10681 tcaagtttcc cggcaatcac cttgcccgca tttacggggc cggagccttt cagactctgg
    10741 taaatcgcga tctgatccgc cccaccaacc gcctcttcat cattggaggc ggcaacgtgg
    10801 gcctcatcgc tgcctatcac gctttgcagg cagggatcga ggtcgtgggc ctggcagaag
    10861 cgatgcccgt ctgcggaggt tataaagttc atgccgacaa gcttcaaaga tttggggtgc
    10921 cgatctacac ctctcacagc atcctctgcg ccaacggtga agagacggtc gaatctgtca
    10981 ctatcgccgc cctggacgat aagttcaaga ccatcaacgg cacagagaag agctttaagt
    11041 gcgataccat cctgatcgcg gtgggtttgg attccctttc ggaattcact atcgaagcgg
    11101 aagcggcagg catacctgtt tacgctgccg gagacgcttt cgagattgcg gaagcctcat
    11161 ccgccatgtt taacggcaag attgccggcc tgaagatcgc ggccgacagc ctggccgggc
    11221 aagcagacgt ggaagcaatt cccgaagaat ggtatgccaa agctgattta cttaaatctc
    11281 acccgggtcc cgtgaaaggc tatcagaacc atggcgccga gacgggtgtc ttccctatca
    11341 tccactgttt acaagagata ccctgcaatc cctgcaccac cgtctgcccc accaattcca
    11401 tccaaaccga ggatggcggc ctcatggcag ttccgctcta caagggctcc tgcatcggat
    11461 gcaacaaatg cgtgctgatc tgcccaggcc tggccataac tctggtggat taccgcaaag
    11521 atccggaaca ccccaccgtc actatcccct acgagatcag caatttcaag cgccagaaag
    11581 gtgacctgat cgatctggtg gatatcgatg gcaatgatct gggaagctac gcgatcgaca
    11641 ccgtgctgga tttcaaagac cgccacaccc aattggtcaa agtgtctgtt cccaaagcga
    11701 ttgccaaaca ggtagccgcc ttccgggtcc aggataagca ggtgagtgag cccctggcgg
    11761 aagccatcat ccctcaaaag cttggcgacg atgccatgat ctgcctctgc gaacgcgtca
    11821 gcgtaggcca ggtgaggcaa ttgatcagaa gcgggattac cgatttgaac cagatcaaag
    11881 cagttaccag agcgggtatg ggcccctgcg gtgccaaaac ttgcgaaacc ctgatcaaag
    11941 gcgtgctgag agaggagggg attcccaccg aatctgtggt ccccaacacc aaacggcccg
    12001 tctttattga agtcgccctc gatatcttcc cggatggagt ttaacatgaa caacacttat
    12061 gatttcatca ttatcggggc cggatcgatc ggattgccgg ccgctcttga cctggctgaa
    12121 aaagggcaaa aagtgttggt gatcgatccc gaacacggcc ctggccagga aaacaacaag
    12181 aaagccatcg gaggcgtgcg agccactcac agcgactatg gcaagatcag cgtctgccag
    12241 agatcgatag atattctcaa gcactggaaa caggagcgcg gcgacgatat cggctggatg
    12301 tcaaacggtt acagctaccc cgcctgggag gccaaagacg cggacgccct tttggccctg
    12361 atgaaagtgc aacacagctt tggactcaat atcaaatggg tgagtgccga ggaatacaac
    12421 gaactggtgc ctggcatcaa tatggaaaac ctcatcggct ccacctattc tccggaggac
    12481 ggttcctgct ctccactcat gctttcatca tccatctatt ttcacgccct gcggaaagga
    12541 gtcgaattcc gctttggtga aaaggtcgtc tcgttttcca agcgagagga tcggatcatc
    12601 agctgtaaaa ccgataaaga tgagtatcag gcgggtggat ttgtcaatgc tgccggaaac
    12661 tatgcccggg agatcggagc tatggctggt tccgacatcc aggtaggtcc agacaatcat
    12721 gaagccggca tcacagagcc tgtggcccgc ttctttggtc ctatggtgat cgacatgcgc
    12781 aagcgtcccg gatctgccaa cttctatttc tatcagaacc acgaaggcca ggtctgtttt
    12841 tgctacactc ccgagccacc catcctgggg gtcgacaacc gcagcaccag cgagtttttg
    12901 ccccactgct ccaaacgcat gctggaagtc ctgcccagac tgcgctacct caaagtgagg
    12961 cgcacctgga gaggccagta tcccatgact ccggacggtt ttccgatcgt gggcttcaaa
    13021 caggaatgct ccaatctact gcacgccgtt gggatgtgtg gacagggatt tatgctggga
    13081 cccggcatgg gagagttgat cagcagaatc tgcttgaaag aaaccagcga cagcgatgcc
    13141 aggatcctga aaagcttcga tccctacagg gattttagcg gcatggaagc ctttaagtag
    13201 aaatatcccc catctatcct taacctggcg ggtagtttaa ccgccaggtt ttttttgcca
    13261 gccgcctctc ctcacctatt cgtttaccgc ttggtcctct ggagctcacc ctacccatcc
    13321 cgaacttgat aggggatgcc ctgggctatc tataggcagg cgaagggtcc atgggtaagg
    13381 gcgaagaggg aaatcaagtt cggatgccca taaatcgcat cattgtcctt gccgattagc
    13441 cgctcttgcg atgccgtgcg ctctccggcg agatttccca ccggcggcac ggcagagcta
    13501 cggcatgaaa cgggaaagga atgtaaaaca gccggcccga tggttacggg ccggcttgac
    13561 tatatgattg attcgaggtt attggacctg cgaaatcatg ttgttgatgg aagagatgcg
    13621 gctattgatg tcgctcaggg ctgaggagtc agtagttctg gagcgacctg cttccagctg
    13681 ttctttcgcc ttgcggagga agttcagagc ggaagccttg gcggcgtcgc ggtctttctt
    13741 gagctggttg gcttttttac ccacggcttt ggcggcttgt ttttccagat cggcaaagcg
    13801 gttgtattga tcggttccct tgctctgggt cacagacgcg aggatcacgt aaggctggta
    13861 gagtgagggg tttttggcaa tggcggtatt ggcgtttgat tccgcttcgc tgttcttttt
    13921 ctgagaaagg tagatggcgg cgagattcag atagaccatg gcgttttcgg gatcaatctt
    13981 cttggcctga ttgagagcgg tgatggcttt ggtattcagc tcgttggtca ctttgctgtc
    14041 cctgctttca cccgcctggg ttctgtaaag ccccgcgatg gtcagatagg cctggatgtt
    14101 ttgaggatcg cttttgagga tgttttcata gcgggtgatg gcttccgtga gacgattggc
    14161 tttgaaatag gacgcggcca gacggtctga gaccagctgg ttgtccggga aaagatcagc
    14221 cgctttttcc agttgaggga tcgcacccac atagtcacct tcttcaaaga gcataaagct
    14281 cagacgtata agggcctgtg aataatcagg attgatctcc agagatttct ggaaggattc
    14341 cttggccttg gctttgttgc cggcttcatc atagaggttg ccgatcgcca gccacagctc
    14401 ctcatcggga ttcttgacgg ccatagcttc catgagtttc aagcgtttgt ccgtctgctt
    14461 ggtgatctca tacatttcga tcagcatgag ctgggcgcgt tggtcctcgg tgttgagcgt
    14521 gatggctttc tcgaggttct gttcagcttc agtatacttt tcctgtttgt aataggtggc
    14581 tcccaggtag tagtaggcgt cccctttgtc tgggttgaga ccaatcacgg aattgaagcc
    14641 gcgaacggcg tcttcatatt tctgggtcac atagttttgc accgcgatgt tgaggagctt
    14701 gtccacttca ttggagataa agcggtccag ctcaggcaaa aagtcttcct gcatcatggc
    14761 aaggcgctga tcgcgattgg taaccacatt gaaagtatgc tgtttgagct cgtcagaacg
    14821 gctgctgtag aaacgggtgt tgaccgcgaa gaggccagga cggctttcac tcacgttgcc
    14881 aataatcacc acgtccgagc cggtaagctt ggtcatctgc atgagctgct ctttttccag
    14941 ggcgtccaca ttggtaaatc ccgaatcttt aaactgcttg gccaccacgt ccatatccaa
    15001 aagcgaatat ttgggatggg agttaaaggc cagggccagg tctctgatag acatgatttt
    15061 tactacattg cggctaccgg aatcagccgc ctttaaggga aggatcgcca ccttggtgac
    15121 ggttccggtg aaagcgggta cgggctcaga actagtctgt accttattcg aggtgcagga
    15181 aaacagcaga agcgctatcg tcactgcgat caggatccga tgggtcatta ttcctccatg
    15241 ttcttatgct tttgatatcc gatttcaaaa acaggggcta tctgtcaagg aaaaagggct
    15301 tgttacccac tatcagtgag ttatgaactc acattgcagg ttctgaaccg atcatgggca
    15361 ttatttcatc gatggagcgg ggggagtgac ggtgccatcc aaaaacgcaa agggtgccac
    15421 cagatatagt gacaccccac agtcggaaga tgcttagatt agatcttctt atggcaaaac
    15481 caccagtcaa agcactcgaa ttcgcctttg gcagcctgtt ctttgcgttt ctgagcacta
    15541 tccacgtctt tggcgggagg gatgatgata tgatcaccta ccagctcgtt cttgggccag
    15601 ttggctggca tggcgcagga attcttgtcc gagaactgca tggcttccac cgctctgaga
    15661 atctcttcca tgttgcgtcc cagctcctga ggataataga atatgatcct gatggcggct
    15721 ttatcgtcca cgataaatac ggcgcggacg gtgttggtgc ccttgccggg atggatcagt
    15781 ccaagcttgg aagccactgc tcccgtatct gcaataatcg gaaactggat gtccactttg
    15841 agattttctt tgatccattc ttcccatttg atgtgtgaaa agacctgatc aacgctcata
    15901 ccaatcagtt cgcagttcag cgctctgaat ttgggataca gattttgaaa ggctacaaac
    15961 tcggtggtgc agacaggggt aaaatcagcc ggatggctga agagcacaaa ccacttgcca
    16021 gccatagctt tggggagttc catttcccca cgggtggtct ggactttcat atcggggaat
    16081 ttgtcgccca agaggggcat gccttgcttt tccatgtttt ccatgtgttt ctccttaatt
    16141 gatttgattt acatatatat tataagcaat aatccgggaa tgtcgaccgg tctggggagt
    16201 agctgagcac gacagccaca gaagcgctgg gggcttatca tcttgcgggc tgaatagaaa
    16261 ggcccaagca agtcaatcct gacggtggat tatcccacga gggaaacggg agtccacctc
    16321 attgggaata ttttatgtga ctaccctgat cagctaagcc ggggcctgcc aatcgcgtag
    16381 taatcgaagc ccagagcttt catactcctg ggttcatact ggtttctgcc atcgaggata
    16441 agaggatgct tcattaagct cttcacgcgc tcaaaatcgg gataacggaa ctggtgccat
    16501 tcggtcacca gcagcatcgc gtcggtgtct ttcaccgcag tgtattcatc cggcatcaga
    16561 tcgatccgtt caaggcctct gaaaacctca gcggccactt tcatggccac aggatcgaaa
    16621 gcctggacgc gagcccctcg ttcgatcaaa gccctggcga ttacgatcgc aggcgcttcc
    16681 cgcatatcat ccgtctgagg cttgaaggca aggccccaga gggctactgt cttaccgctc
    16741 atatcatctc cccacagcga gatgagcttt tctaccagca ccatcttttg gcgacggttg
    16801 gtgtcttcca ccgcgtccag gatgctggaa atggaaccgt tttcgcggtg catactgatc
    16861 agagctttca cgtccttggg aaagcatgaa ccaccgtaac ccactccagg atagataaat
    16921 ttgttgccta ttctgctgtc ggaaccgatc ccagccctca cttccgagat atccgcctga
    16981 agtgtttcac acagccggga aagctcgttc ataaaggata tctttgtggc cagcaacgcg
    17041 ttggccgcgt atttggtcat ctcagccgaa cggacagcca tcacgatgac tctgtcggcg
    17101 ctgcggttga agggtttgta gagctccctc atgatagcgg cggtctcttc gtcccctact
    17161 cccagaatca ctctgtccgg tttcataaag tcttctatgg cagcgccttc tttcagaaac
    17221 tcgggattgg agaccaccga gaatttgtgg cccaccccac gctctgaaag gacactctgc
    17281 accgtccggg tcaccaggtc cgccgttccc accggcacgg tggatttgtt gacgatgatc
    17341 ttggacccgt tcatataaag agcgatctca cgcgccgccg cgatcacgtg gctcagatct
    17401 gccgagccat cttcgtcggg tggagtgccg accgcgataa aaatgattct ggacgcctca
    17461 acggcacttt gaagattggt ggtaaaagtc aaacgcccag actggtgatt ggcgatcacc
    17521 atttctttga ggccgggttc gtagatggga atgtctcccc cgttgaggat gtcaatcttg
    17581 cgagggtctt tatccacgca aatcacggca ttacccatat cagcaaaaca tgtgccggaa
    17641 accaggccta catatccact gcctatcact gcaatcttca cgatggggct ccctgttttt
    17701 taaaatacgc gatggtctct ttgacgccat cttcgaggtt gtagatgggg ctccatccca
    17761 gaacgcggta ggccttggaa taatccaaca tggacctgcg cagatctcct tttcttgctt
    17821 cgcctcgatg aggcggcaca tccagcccca gtttccactg gatggtgtta tataattgag
    17881 tggtggtagt ttccacacag gtaccgatat tgaagcgatc gttgttgcct tcatgcagtg
    17941 ccagcaggtt tgccctcacc acatccgcca caaacacata atcgcggatc atgccctccg
    18001 gctcatccgg ataagcgtaa acggttggga tcaggccctg cagcaatttt tcgatgaaga
    18061 tagaaacgac tccagcctca ccgtgtgaga cctggcgggg accatagacg ttggcgtagc
    18121 gcaagacggt gtagttcagg ccatactggt ggtgatagag gtgcagataa tgctcaccgg
    18181 ccatcttgtt gatcgcgtat acggaaagag gagtgggatg atagttctct gtggtgggat
    18241 actcgtctgc ctcgccataa atagctccgc cagaagatat atagatcact ttcttcaccc
    18301 gatactttac acaacactgc agtatattta taaatcccaa tacattgaca gaagcgtcaa
    18361 actgaggatc ttcaatagag agcggtaccg atatctgggc ggcgtgatga ttgaccaggt
    18421 ctggctgctc gctggcgaaa atttccgcca gttggggatc gcggatatcc agtttataga
    18481 atttggccct gggatcgagg ttgtgtcggt agccggtgga tagatcatcc actaccacca
    18541 cttcgtgtcc ctgatcgaga taggcctgcg tgacatggga ggcgataaat ccggccccgc
    18601 cggtgactaa aatcttcatg taaacctcat atctttagtg agatggctac gccatgtgag
    18661 atacttcgta ggtgagatac tgtgtaggtg agatacttcg taggtgagat acttcgcagg
    18721 tgagagcagt actgcgagat gctgagcatg gaaaatcaat cggtctccac gacttcatat
    18781 tcgccgatga tcctgatgtt ttctggttca accttcacca gaacggcctg acgcaggata
    18841 ttgacctgca gacggattt
//
LOCUS       DAOSFN010000035        18787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2389_length_18787_cov_9.374386, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000035 DAOSFN010000000
VERSION     DAOSFN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 18787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18787
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2389_length_18787_cov_9.374386"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..1001)
                     /locus_tag="PLO35_03745"
     CDS             complement(135..1001)
                     /locus_tag="PLO35_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78809.1"
                     /translation="MRKLALLLTALLSLGCLMALSLDISGPDDKVWDIEQLRTQQLGS
                     FNTIRVKGIEKVEDNWQGLELIPWLEAQTDYVWHELEFVSVDAHSLRMSRLDLESKPV
                     WLALKDSQGWLQEGIRLVFPGQRDNIWIRGLDKIVLHGFTPLPAPRRIYSWEDVNLPQ
                     DESGISSVSDLVRSFFAESQGDLLIVSKDGTPLRLKYPEHLIEAQLVWKDGTLAIQPE
                     ALLLPGMPPALIYLQMGHQAILTPDGVKLLPDLARALQWPESPGWKAVRPLYEKYEPG
                     TRIPQDGWLERD"
     gene            complement(1231..2997)
                     /locus_tag="PLO35_03750"
     CDS             complement(1231..2997)
                     /locus_tag="PLO35_03750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH78810.1"
                     /translation="MKRFLILLALTGSLLWAQTPDPKAIIGKIDDKVYTYAEYETILS
                     DYFKHFNPTHKKLSVEEQARLNDQCWEELIGRYVYDQAIKAGKIKLTNAQVLAEAKKN
                     PPAAVKQIKDLQTKGKFDKQKYLDALNHAPEFKKAVIAEVRALYQYTKLLDTIKAEVD
                     ISADSVKAKWFKDNDTVDARIIYFDYHRLTEVVASEDEARRYYEERKDEFKKEDGRSL
                     RYVKFSKTPSAADSLAARQEADRLYEELLAGRDFAIAAQELSQDPGSGQNGGSLGWFG
                     RGRMVKPFEETAFSTPIGVISRPVLSNFGWHIIKVTGRREGQNGEEVEASHILLSVNA
                     SEKTLQDYKINSARLHAEAQQRGLEKAANELGFELTVTPAFFAKEGFIPDIGREAKLI
                     TFAYENPPNTLSDIYYASSGDAYVIETAEQFPVWYPDFETEKANFINRATTTKRMYTM
                     SQIAEGYISELKPTEYLSQADQDSLTVIQITGHKDGDTITSIGKVEELNAALFATPQG
                     QFTPLIKNNNRWFLAFIDKRSNPDIKAWEKLKTKEIASAREELRKQHLNNWYASERKK
                     VSIIDNRADYYDLSSTRKMIKL"
     gene            3432..6290
                     /locus_tag="PLO35_03755"
     CDS             3432..6290
                     /locus_tag="PLO35_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH78811.1"
                     /translation="MTKLTRLILPVLMLLASGALSAQFAQGLFFSEYVEGSSNNKAIE
                     IFNGTGQAVDLSSYTVKLASNGNTWSTTNIHAMTGTLAHNDVYVIANASANAAILGVS
                     DATSTVTYFNGDDALGLFQGDTLIDIFGIYQNDPGTAWPVAGVADATLNHTLVRKPTV
                     TSGVTDWSVAAGTNADDSQWIVQAIDFITDLGTHTFTPGGGNNTATPVFDPTGGAYTA
                     PVTVTITCATAGASIFYTSDGSTPSVASTPYTAPLNISTATTLKAIAIAPGMDPSYVA
                     TAVYSFPVTVPNLSALRSSPADGTTIYHVSGEVILSFKQIFRNQKFLQDAGAGILVDD
                     LNGVISTVYNVGDGITGITGKISEYGGMLQFVPTSNTPAASSTNNPIVPVVVNFDQMV
                     NAFDTYESRVVKVMGVEFVDPTGNFANGTVYACYDQDTDYNIRSSFYDVDYINTPIPT
                     SPKDIVGIPNSRTDGNYFTPRSLADFIDPAGTVAAPVFDPNGGVFLNPVSVSMSSATA
                     GASIHYTLDGSAPTAASTQYASPITIAATTTLKAIAVLPGSPSSGITTATYSFPVTVT
                     NLSALRSAPLNGLYRVQNEVLLSFKQSFRNQKFVQDDQAGILIDDLNGVITTNYQVGD
                     EIGNVVGTLTEFGGMLQLVPAQDFGQAVSTENPIQPILITIDQFINSFDTYESRLVTI
                     GNVIFSDPESNYANGTVYPFTNVTATQTANFRSTFYDVDYIGIHIQPWVINMTGIPNA
                     RTDGNYFTARSMADFQINDEMPPLLFNATVNDPHSVSFEIGFEAVNNVTLPVGLTGYK
                     LYRNSVAIQVLPADLHVTFTDTTMPNGTSTYYATAVYGPYESEPTSEYVHIITSTDDP
                     SAPASVTQLKGNWPNPFNPSTTISFSLKDAGQARVDIYNSRGQIVRTLLNETKAAGEH
                     SIEWNGKDDSGRELGSGIYFFRMLSGKYSSTRKMLMLK"
     gene            6304..7179
                     /gene="htpX"
                     /locus_tag="PLO35_03760"
     CDS             6304..7179
                     /gene="htpX"
                     /locus_tag="PLO35_03760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOH78812.1"
                     /translation="MKGFKRLGIFLLTNLLVMTMLSVVMSFINVPMDQLWGLLIMCAV
                     FGFGGSLISLLMSKFMVKMAYKVQLVDPASASGKLRFVVDTLARMAEYHKIRMPEVGV
                     FPSDAPNAFATGASKNKALIAFSSGLLASLSEEEIAAVAGHEMTHVIEGDMVTMSLMM
                     GLVNTFVMFLARVLAFALDMALRDNRGRGGLGYFGYMLVVIVLQNVLMLLAYIPISYF
                     SRQREFRADAGAAKLVSPGAMIDALKGISGNRQPVSSKATAADMAMIDNKRKVSLFST
                     HPSIEARIERLQKLL"
     gene            7176..8543
                     /gene="rlmD"
                     /locus_tag="PLO35_03765"
     CDS             7176..8543
                     /gene="rlmD"
                     /locus_tag="PLO35_03765"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOH78813.1"
                     /translation="MKIISGLRIEKHALGGYGLGFADGKAIFVPYAHPGDEIDAEIIR
                     ERKDMAFARVRHYRLRAEIPRDGLCTSFGPEAACGGCDWQDLSYASQLRYKLDLLTEL
                     FSGSGFEAVPKALIPSPQEVHYRNKVFMPVTSTSEGLIFGIFERNSHKVVPHHSCRIH
                     PPIFDTLALRVAQICQQAGVTAYNEKSHNGHLRHIGIRINQDESQLLLILVTRGSKLP
                     FSNLLIKKLTEEFPALVGIVQNINRERGNVILGDEEKPLWGKPYLSDTLAGKQFRVQY
                     RSFWQVNTPVTELIIARIRKQLEPGCTLIDAFSGCGSIGISLADKTRQTICVESNQAA
                     VTDGEHNATRNGVENIGFVCARCEDALPTLLEDPTINVAPLNIVLDPPRSGVLRPALD
                     AIIASNASKIIYLSCSPITLARDLKILVNEGGYRLSRLQPFDMFPQTWHIECLAFLEK
                     QTCVT"
     gene            8531..8959
                     /locus_tag="PLO35_03770"
     CDS             8531..8959
                     /locus_tag="PLO35_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOH78814.1"
                     /translation="MRYLIPILLVLSMLNSSCGISDPQIGAEQEIRDLIYDVTQAYNW
                     GDIDAIMELVHPEFRHEGMYSLQLRQLWLDRLARFPLLETNVTSVEVNGDYAVAHFSM
                     LFTSSTESILSTEPQDNGDLSYFFYDSGKWQIYGDQGWIK"
     gene            complement(9577..11385)
                     /gene="typA"
                     /locus_tag="PLO35_03775"
     CDS             complement(9577..11385)
                     /gene="typA"
                     /locus_tag="PLO35_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012861919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOH78815.1"
                     /translation="MRKIKNIAIIAHVDHGKTTLVDAMLRQAGVFRDNERLVERVMDS
                     NDIEKERGITIFSKCAALEYKGYKINLVDTPGHSDFGGEVQRIMKMVDSVLLLVDAFE
                     GPMPQTKYVLKNALDLGLQPIVVINKIDRPNARAYDVLDMIFELFLELNATHEQLDFA
                     VIYASGKDGYAVDDPNDEPLNIFPLLDMIIKEVPDTAGDRKQPFQMLTSAIEYDNYLG
                     KLGTGKIHSGTVKTGQEVMLIKRDGSIIPYKVNALFVYEGTHKKEVKTAYAGDIITIA
                     GLEQIDIGETIAHEDNLVPLPSISIDEPTIAIEFIINDSPFLGRSGKWVSSNKIWERL
                     QKELQTNVSLIAEKTESSDTFIVKGRGELQLSILMENMRREGYEFQVSKPQVLFKEKD
                     GKITEPIELAMIDVSEEFVGTVIDMMGGRRGELIHMTPPTDGYSRLEFKVPSRGLIGC
                     RNEFLTQTRGTGIMNQFFLEYDFYKGDINGANHGSLVSMETGIALGYSLNNFQARGTF
                     FINPNTEVYGGMVVGQHSKEKDLVINVCRGKKLTNNRAAGKDDAIKLIPPRIFSLEQA
                     IEYIGEDELLEITPDSIRMRKKILDHTERKRSEIAD"
     gene            11620..12678
                     /locus_tag="PLO35_03780"
     CDS             11620..12678
                     /locus_tag="PLO35_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOH78816.1"
                     /translation="MKTGGCRYGTHRVIEPQGVLPQPARILNNDMSEIWDNELLIDVI
                     RLNVDSASFHQIKNKLVAQGHTDIEKAFAEHAIDLTTRTGKHKNEDTGSGGMLIGRVA
                     AIGDKFEMKDKIKVGDKIASLVSLSLTPLKISKIKQVLLDKDQVEIEGQAILFSSGIY
                     AKLPDDMDENLALSVLDVAGAPAQVERLVKPGDTVVIIGANGKSGILCNAVARERAGI
                     SGKVIGVVRNQSYVPTCLETGCHEVIIASATDAITIQKEVSRLTDGKMADVVINVVNV
                     EDTELPTIMACKDRGIVYYFSMATSFTKAALGAEGIGADVDMILGNGYAHHHAMISID
                     LLRRNPVLMKLFKERYTD"
     gene            12689..13963
                     /gene="ablA"
                     /locus_tag="PLO35_03785"
     CDS             12689..13963
                     /gene="ablA"
                     /locus_tag="PLO35_03785"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOH78817.1"
                     /translation="MNPAKISGERELTIPIRSIATDAQWQDWHWQLRNRITDFDTLSK
                     YIKLLPDEKAVADSSVFSFRMAITPYYLSLIDPSNPNDPVRLQAIPRIAEGNISHVDM
                     ADPLHEDADAPVPGMTHRYPDRVLLLLTDQCAMYCRHCTRRRKAGEHDAPMPRENVDK
                     AIQYIKEHKEVRDVILSGGDPLTLGDERLDEVLGKLAEIDHVDIVRIGTRLPVVLPQR
                     ITDSLLGVLRKYKFVWLNTHFNHPNELAEDNVKALARLAETGVVMGNQSVLLKGVNDH
                     VDVMKQLLHKLVRNRVRPYYIYQCDLSEGIAHFRTPIAKGIEIMESLRGHTSGLCIPT
                     YVVDAPGGGGKIPVMPNYIISQMPGRVILRNYEGFITSYTEPGYEAQKDDNYRELCPE
                     ERRSSDGVMSLLRGKKVSIGPADTRRNLRRKQ"
     assembly_gap    14065..14164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14239..15285
                     /locus_tag="PLO35_03790"
     CDS             14239..15285
                     /locus_tag="PLO35_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78818.1"
                     /translation="MSFAWISDDGVRVIAIVGICKNAGKTSLLNHLLRACSDKNAYGD
                     VFESKIRFGVFSTGIDGEERDSVFNTAKPAVKLPANTLFCCDAMALDSLGGEVEIQAS
                     LSNPQRPLWIARSLYRLDTMITGPASVVDQIELSRKMLALGAGKILVDGSLDRKSIAL
                     SPDVDAVVLVLGASYGNPAKLETELKRLLRLAALPVFDTGPDEYKLLLGSDELLTSDG
                     RTWQSTSLQTLSGKESELDKMLRNNLSSSRLYIPGAVTNSSLDALLSLLPKRPYTLVL
                     RHPDCLKLDISSLQKLAERVELTTLIPFRLKGFAINSEGVGLASEDAARFRSRLRTSF
                     PQLDLIDIMELTHA"
     gene            15278..16192
                     /locus_tag="PLO35_03795"
     CDS             15278..16192
                     /locus_tag="PLO35_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOH78819.1"
                     /translation="MLERDKLTTTTVNLGLGANILLSFAKTLVGILGHSSALLADGIN
                     SSSDVVYYVAVKIFMHQARKPADAEHPYGHRQLETISAIVVGAFILTTGIAILWDSIN
                     KVFDLLSGYTVGRSASILALWIAIGTLALKLYLYHFTRRTSRRINNPTIRALANDHLN
                     DIMASLAVIIGVVLGQLGYFWMDPAAGAIVAIYIIKTGIGIIMDSSGELMDSVPDPEF
                     GKLVRSLALRVEGVRRIEELGVHRFGPYFTINMTIGVNGRIAVEEGHKIAHSVEAALL
                     NNFDSSLRSVHIHYHPAELTESNPMEKS"
     gene            16189..17457
                     /locus_tag="PLO35_03800"
     CDS             16189..17457
                     /locus_tag="PLO35_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOH78820.1"
                     /translation="MNPGRNILLGLVLFVLLGFSVYSVFDPDRPHRAKTHHISTGQDS
                     LKTDPWIVEIVPDGGSLFGIMEKHQIPLREIATVTYQFSDFIDVSTIQPGDTLKLKLS
                     PSKDKIEAMTFVQEPTIRHQFIVQGDSLVYNEEKLPVQEEIKLLAGKLEGTLSASLDR
                     LGLPNQDAQQIINALEAKINFRRDAKTEDEFQILAQLRYFEGKLIPGGKVLYAAYHGK
                     KAGKHELFRYEDPNPKSVLTGLYGPDGKIVRSTSGGGVGYPLGSIHVRSKFGRRIDPV
                     YGHWAMHQGVDYKASYGTPVMAVANGTVTEAHYAGGWGNQVRIKHPSGLTSQYAHLSS
                     IAVRRGQSVNKGQVIGRVGSTGKSTGPHLHFGLMKGGQWINPTNLKMVASEQLTVIQL
                     KDFTRQRDLIKAQLQQYLNPPAPVSATVKT"
     gene            17438..18604
                     /locus_tag="PLO35_03805"
     CDS             17438..18604
                     /locus_tag="PLO35_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOH78821.1"
                     /translation="MLQSKPDKSGYEYQRTGRYFIQVAGSLERLARAELLDLGANIHT
                     EVPRGFFVSCNLETLYRIIYTSRLCQRVLAPLVSFACHSEKYLARQSASLLDWTSLFE
                     VDSSFGIDVNLSGSKIKHSLYAGQLVKDAICDQFRAQSGRRPDFRTTNPDINFNLHIK
                     DNYATLALDLSGSMHKRGYRSGPSDAPLQETLAAAIVKLSGWDGRNPLSDPMCGSGTL
                     LCEALMSWCGIPAAILRENKYIERFPDHDPKLWKELRAQANQAMRELPDGLISGSDVS
                     ATALAVARASLDRLPYGRGVKLVKSRFQELPSQAGRCVITNPPYGVRLEESASVAKIY
                     NDLGDWLKQRCPSSESYILCGSADLASQLRLRAWWKKSLKNGDLEVKLAKILIR"
BASE COUNT         4352 a         5136 c         4865 g         4334 t
ORIGIN      
        1 ccgtcccgct gggactcaat acgcaatcat cctactagct ctatagccag ttatacaaat
       61 atgtgcagca gcatctgagc tgattcgcgg catgcggatg agatcacata tcaaacacat
      121 gaagacgttt gccgttaatc tctctccagc cagccgtcct gagggatccg ggtgccgggc
      181 tcatactttt catagagcgg cctgacggct ttccagcctg gtgattcagg ccattgcagg
      241 gccctggcca ggtcgggcag gagcttgacg ccgtcgggag tgaggatggc ctggtggccc
      301 atctggagat agataagcgc gggtggcatg cctggcagga gcaaggcttc aggctggatg
      361 gcaagggtgc cgtctttcca gacaagctga gcctcaatga gatgctccgg atatttgagc
      421 cggaggggtg tgccgtcctt cgaaacgatc aataagtctc cctgggattc ggcaaagaaa
      481 gatctgacca gatcagagac tgagcttatc cctgattcat cctgaggcag atttacatcc
      541 tcccaggagt agatgcgacg cggagctgga agaggcgtaa aaccatgcag gacaatctta
      601 tcaaggcctc tgatccagat gttgtcccgt tggccaggaa acaccagccg gatgccctcc
      661 tgcagccagc cctgggagtc tttgagggcc agccacacgg gtttggactc caggtccagt
      721 ctggacatcc tgaggctgtg tgcatccaca ctcacaaact cgagctcatg ccagacataa
      781 tcggtctgag cttccagcca gggaatcagt tccaggccct gccagttgtc ctccactttc
      841 tcgatgcctt tgacgcggat cgtgttgaag ctaccaagtt gctgagttct aagctgttct
      901 atatcccata ccttatcatc tggaccggag atatcgaggc tgagggccat caggcacccc
      961 agactcagca aagccgtcag gagcaacgcg agtttacgca agcccaagcc actcaggcgc
     1021 ggtgataagt ttcgcgcaga tctcgcagat ttcacgcgga tagcgcacat attgataagg
     1081 ttgataagta ccggcacgcg ctgtagcgtt gacctccgta ccatctgcga aatccgcggg
     1141 gaaaatctgc gtaatctgcg gaaacctgta ccgtcacaat cctgggagcc ggcaaagcag
     1201 gcagaattga gcttcaattg aggcatgatc tcacagcttg atcatcttgc gggtcgagct
     1261 cagatcgtag taatccgcgc ggttgtcgat gatggacacc ttcttgcgct cggaagcgta
     1321 ccaattgttg agatgctgct tgcgcaattc ttctctggcg gaggcaattt ctttggtctt
     1381 gagcttttcc caagctttga tatctggatt gctgcgcttg tctatgaagg ccagaaacca
     1441 gcggttgttg tttttgatca gaggggtgaa ctgaccctgg ggagtggcga agagagcggc
     1501 gttgagctct tccaccttgc cgatggaggt gatggtgtcg ccgtctttgt gaccggtgat
     1561 ctgaatcacg gtaaggctgt cctggtctgc ctgactaaga tactcggtag gtttaagttc
     1621 actgatgtag ccttccgcga tctggctcat cgtgtacatg cgtttggtgg tggtggcgcg
     1681 attgatgaaa ttggcttttt cggtctcaaa atcgggatac cagacgggga actgctctgc
     1741 cgtttcgatc acataagcgt cccctgaaga ggcgtaatag atatcagaga gggtgttggg
     1801 agggttttca taagcgaaag tgatcagctt tgcttcacgt ccgatgtctg gaatgaagcc
     1861 ctctttggca aaaaacgccg gtgtgacggt aagttcaaaa cccaactcgt tggctgcttt
     1921 ttccaggcca cgctgttggg cttcggcatg cagcctggcg ctgttgatct tatagtcctg
     1981 caaggttttc tcactcgcgt tcacactcaa caggatgtgg ctggcttcca cttcctcccc
     2041 attttggccc tcgcggcgac ctgtgacctt gatgatgtgc cagccaaagt tggaaagcac
     2101 cggtctggag atgacgccta tcggggtgga aaaggccgtt tcctcaaaag gtttgaccat
     2161 tctgccccgg ccaaaccagc ccagtgaacc gccattctga ccagaaccgg gatcttggga
     2221 aagctcctgg gcggcgatcg caaaatcgcg gcctgccaac agctcttcat agagtcggtc
     2281 agcttcctgg cgcgccgcca gagaatctgc cgcggagggg gtcttgctga atttcacata
     2341 ccgcaggctg cgtccgtctt cctttttaaa ctcgtctttg cgctcttcat aataccggcg
     2401 ggcctcatcc tctgaggcta ccacttcagt cagacggtgg taatcaaaat agatgatgcg
     2461 ggcatcgacg gtatcgttgt ctttgaacca cttcgctttg acgctatccg ccgagatatc
     2521 tacttcggcc ttgatagtat ccagaagttt ggtgtattga tagagggcac ggacttccgc
     2581 gatcacggct tttttaaatt ccggggcatg attgagggca tccagatact tctgtttgtc
     2641 aaatttgccc ttagtctgca agtctttgat ctgttttacg gccgcgggag gattcttttt
     2701 ggcctcagcc agcacctgag cgtttgtgag ctttatcttg ccggctttga tggcctggtc
     2761 atacacataa cggccaatca gttcttccca gcactggtcg ttgagcctgg cctgctcttc
     2821 cacgctgagc ttcttgtggg tggggttgaa atgcttgaag tagtcgctga ggattgtttc
     2881 atattcggca taagtgtaaa ccttgtcgtc tatcttgccg ataatagcct tgggatctgg
     2941 cgtctgggcc cacaagaggg aacccgtgag ggccagcaga atgagaaaac gcttcatcag
     3001 aaactcctgt gctgtatctg tatttcttta taacaatacc aacgggtgac caaaaaactg
     3061 cgcggcggcc tgaggtcaag attaattgag aatggaacgc agataaaggg aaaaagggcg
     3121 ggattagttt agaattgaga attgagaatt gatcgtgtaa atcatgtcat ccagttatct
     3181 gtgttcgatg tgattcaaat ccccaaaatc ccttttttcc cttgttctgc gttcagtttc
     3241 atgttccata ctcagttttc aattctccat tttctagaaa ctctgctttt ctctgggttg
     3301 aattgcaccg taagattttt cttgcctgaa attgggcttc cgttaaaatg cctacaaact
     3361 ggatttttgt gcccggaatc tctgtggttt cggatgaaat agtttaaaat cagatacttt
     3421 aggagtttat catgacaaag ctgactcgcc tgatcctgcc cgtcctgatg cttttggcaa
     3481 gcggagctct cagcgctcag tttgcccagg gcctcttctt ctcggaatat gtggaaggtt
     3541 cttccaacaa caaggccatc gagatcttca acggcaccgg ccaagcagtt gacctcagca
     3601 gctatacggt caagctggct tccaacggaa atacgtggag caccaccaat atccatgcca
     3661 tgacaggcac tctggcccac aacgatgtgt atgtgatcgc caatgcaagt gccaatgctg
     3721 ccattttggg tgtgtccgac gccacgtcca ccgttactta tttcaacggt gacgatgctt
     3781 tgggactgtt tcagggtgat acgctgattg acatctttgg catctaccaa aacgatccag
     3841 gcacggcctg gccggttgcc ggagtagcgg atgccactct gaaccacact ttggtccgca
     3901 aacccactgt tacctctgga gttacagact ggagcgtcgc ggccggcacc aatgccgatg
     3961 acagtcagtg gatcgtgcag gccatagatt tcatcactga tctgggaacc cacaccttca
     4021 ctcccggagg tggaaacaac accgccacac cagttttcga tcctaccgga ggagcttata
     4081 ccgctcccgt gacggtgaca atcacatgcg ccacggccgg ggccagcatt ttctacacca
     4141 gcgatggcag cactcccagc gtggcctcca ctccctatac cgctcccctc aacatctcca
     4201 ccgcgactac tttgaaggct atcgcgatag ccccaggaat ggacccatcc tatgtggcca
     4261 cagcggttta cagcttcccg gtcacggttc ccaacctcag cgctctcaga tcttctccag
     4321 cagacggaac caccatctac catgtgagcg gagaagtgat cctgagcttc aagcagatat
     4381 tcagaaacca gaaattcttg caagacgctg gagcgggaat cctggtggac gacctcaatg
     4441 gcgtgatcag cacggtctat aacgtgggag acggcatcac aggcatcaca ggaaagatca
     4501 gcgaatatgg cggcatgctg cagtttgtgc ctacctcaaa cactccggcc gcttcctcca
     4561 ccaacaatcc catcgtgcca gtcgtggtca actttgacca gatggtaaac gctttcgaca
     4621 cttacgaatc cagagtggta aaggtgatgg gagttgagtt tgtggacccc accggcaact
     4681 ttgccaacgg cactgtctat gcctgctacg atcaggatac cgattataat atccgcagct
     4741 ctttttatga tgtggactat atcaacaccc ccatccccac aagccccaaa gacattgtgg
     4801 ggataccaaa ttcgcgtacc gatggcaatt acttcacacc ccgctctttg gccgatttca
     4861 tcgatccggc cggaacagtg gcagctccgg tgttcgatcc caacggagga gtctttctga
     4921 atccggtttc ggtctccatg agcagcgcca ctgccggggc ctcgatccat tacaccctgg
     4981 atggcagtgc tcccacagcc gcttccacgc aatacgcgag ccccattacc attgctgcca
     5041 ccaccaccct aaaagccata gcggtgttgc caggctctcc ctcttcggga atcaccaccg
     5101 ccacatacag tttccccgtc acggtaacca acctcagtgc gctgagaagc gctcccctca
     5161 acggtctcta ccgggtccag aatgaggtgc tgcttagctt taagcagagc ttccgcaatc
     5221 agaagtttgt gcaggatgac caggccggca tcctgatcga cgacttgaat ggcgtgatca
     5281 ccaccaatta tcaggtcggg gacgaaatcg gcaacgtggt gggcaccctc accgagtttg
     5341 gaggcatgct gcagctcgtt cccgcgcagg attttggaca ggcggtgagc acggaaaatc
     5401 ccatccagcc gatcctcatc actattgatc aattcatcaa ttccttcgat acatacgaat
     5461 cccgcctggt gaccatcggc aacgtgatat tcagcgatcc tgagagcaac tacgccaacg
     5521 gcaccgtcta tcccttcacc aacgtgaccg ctacccagac tgccaacttt cgcagcactt
     5581 tctatgacgt tgactacatc ggtatccata ttcagccctg ggtgatcaac atgaccggta
     5641 tccccaatgc ccgcaccgat ggcaactact tcacagccag gagtatggca gatttccaga
     5701 tcaacgacga gatgcctcct ctgctcttca acgccacggt caacgatccc cacagcgtga
     5761 gctttgagat tgggtttgaa gcggtcaaca acgtcactct tccagtagga ctgacaggct
     5821 acaaactcta cagaaacagc gtagcgatcc aggtcctgcc tgccgatctg catgtcacct
     5881 tcacggatac aacgatgcca aatgggacca gcacctatta cgctacggca gtttatggcc
     5941 cctacgaaag tgagcccacc agcgagtatg tccatataat cacttccaca gacgatcctt
     6001 ccgccccggc cagcgtcact cagcttaaag gcaactggcc caatcctttc aaccccagca
     6061 ccacgatctc tttcagtctg aaggatgccg gccaggcccg cgttgatatc tacaactcca
     6121 gaggccagat agtgaggacc ttgctcaatg aaaccaaagc ggctggcgaa cacagcatcg
     6181 aatggaacgg caaggacgac tccggacgcg aattgggcag cgggatatat ttcttccgca
     6241 tgctgagcgg taaatacagc agcacgcgta agatgctaat gttgaagtaa ggagcttaaa
     6301 ctgatgaaag gattcaagag gttaggcatc ttcctgctca ccaatctatt ggtgatgacc
     6361 atgctgagcg tggtcatgag tttcatcaat gtcccgatgg accagctttg ggggctcttg
     6421 atcatgtgtg ccgtgtttgg ctttggcggc tcgctgatct cgctgttgat gagcaaattc
     6481 atggtcaaga tggcctacaa ggtccagctg gtcgatcctg cctccgcgag tggtaaactg
     6541 cgttttgtgg tggacaccct ggccagaatg gcagaatatc acaagatcag aatgccggaa
     6601 gtgggagttt tcccctccga cgctcccaat gcttttgcca ccggggccag taaaaacaaa
     6661 gccctgatcg ctttctccag cggactgctg gcttccctca gtgaagaaga gattgccgcc
     6721 gtggcgggtc atgagatgac ccatgtgatc gaaggggaca tggtgacgat gagcctcatg
     6781 atggggctcg tgaatacctt cgtgatgttt ctggcccgcg ttctggcttt cgcgctggat
     6841 atggcactac gtgacaaccg tggcagaggc ggtctgggtt attttggcta tatgctggtg
     6901 gtcatcgtgt tgcaaaatgt gctcatgctg ctggcctata tccccatcag ctacttttcc
     6961 cggcagcgtg aattccgcgc cgacgctggg gcagccaaac tggtgagtcc cggtgccatg
     7021 atagatgctc tgaaggggat cagtggcaac cgccagcccg tgagcagcaa agctaccgcc
     7081 gcggatatgg ccatgatcga caataagcgc aaagtctccc tcttttccac gcatccctcc
     7141 attgaagcca ggattgaacg cctgcagaag ctgctgtgaa gatcatatct ggtctcagga
     7201 tcgaaaaaca cgccctgggc ggttatggac tggggttcgc ggatggcaaa gctatctttg
     7261 tgccctatgc ccatccgggg gacgagatcg acgctgaaat catccgcgaa cgtaaagaca
     7321 tggcctttgc cagagtgcgg cattaccgcc tcagagctga aataccgcgg gacgggctgt
     7381 gcacatcttt tgggcctgaa gcggcctgtg gaggttgcga ttggcaggac ctcagctatg
     7441 cttcccagct ccgctacaag ctggacctgc tgacggagct cttttccggt tccgggtttg
     7501 aggccgttcc caaagctttg attccctcac cccaggaagt tcactaccgc aacaaagtgt
     7561 ttatgccggt cacatccacc agtgaaggtc tcatctttgg catctttgag cgcaattctc
     7621 acaaggtggt gcctcatcac agctgtcgca tccatcctcc catctttgac actctggccc
     7681 taagggtggc ccaaatctgc cagcaggctg gtgtgacggc ctataacgaa aaatcccaca
     7741 acggtcacct gcgtcatatc ggcatccgga tcaatcagga tgaaagccag ttgttgctga
     7801 tcctggtgac ccgaggttcc aaactgcctt tcagcaatct gctgatcaag aagctcacgg
     7861 aagaatttcc ggctttggtg ggcatcgtcc aaaacatcaa ccgcgaacgc ggcaacgtga
     7921 tcctgggtga tgaggaaaag ccgctttggg gcaaaccata tctgagcgat actctggccg
     7981 gaaagcagtt cagggtgcag tatcgcagct tttggcaggt caacaccccc gtcacggaac
     8041 tcatcattgc caggatccga aagcaactgg agcctggctg cacgctcatc gacgccttca
     8101 gtggctgcgg cagcattgga atctctctgg ccgataaaac ccgccagact atctgcgtgg
     8161 aatccaatca agcagccgtg accgatgggg aacacaatgc cacccgtaac ggagtggaaa
     8221 acatcggctt tgtctgtgcc cgttgtgaag acgctctccc caccctgttg gaagacccca
     8281 ccatcaatgt ggcgcccctc aatatcgtgt tggacccacc ccgctcggga gttctgagac
     8341 cggccctgga tgccatcatc gcttcaaatg ccagcaagat catctacctg agctgttcac
     8401 ccatcaccct ggcaagagac cttaaaattc tcgtaaacga aggcggctac cgtctttcca
     8461 gactgcagcc ctttgatatg tttccccaga cctggcatat cgagtgcctg gcatttctgg
     8521 aaaagcagac ttgcgttacc tgatcccgat cctgctggtc ctcagcatgt taaactcatc
     8581 ctgcggtatc agtgatcccc agatcggcgc ggagcaggaa atccgcgatc tcatctatga
     8641 cgtgacccaa gcctacaact ggggagatat cgatgccatc atggaattgg tgcatccgga
     8701 gttcaggcat gaaggtatgt atagtctgca gctacgtcag ttatggctgg accgcctggc
     8761 ccgctttccc ctcctcgaga ccaacgtcac atccgtggaa gtaaatggcg attatgccgt
     8821 ggcccatttc tccatgctct tcacctcttc cactgagagc atcctctcaa ccgagccgca
     8881 ggacaacgga gacctgtcct atttcttcta tgatagtggc aaatggcaga tttacgggga
     8941 tcagggatgg atcaagtagt ggcgcttttc caagggctat acatttaact attgagatac
     9001 agagaatttt attttttcac tacagagtta cgaaggaaac agaggagaga tacagagggg
     9061 gttgttttgg ggggggaaga tatttgtaac aaaggaaatg gagcttgtaa gggcggcaca
     9121 tgctgcttga tcgggatcct atcgtagagc agcacaccga tgctgcttga ccgatatccc
     9181 cattgtaggt ttgccgcttg agcataatcg ggtgttctgc tctacatgtg ggtttgcctg
     9241 attatcatga ggttctttat gccaagattt acgctgccat aacgcataaa tgtaaccccc
     9301 aagtccctgc ccagtcatat accgtagaga taccgtgcgc tctcccccga aaatcgccgg
     9361 aggccgcacc gcaactgcac ggcatgtgac tgggcaagga tatggctgga tgcggatgga
     9421 tcagggaaaa aatcatgctg tcgtagatgc ggccttctgt cgcatatctc ccagctatca
     9481 acgatgccaa agaaatcaca gcttacagcc acgcggttac aaaccgcgtc tgcgaaagca
     9541 ttgtgactcc ccagcgaaat actcgctaac taactgttaa tcagctatct cgctgcgttt
     9601 gcgctccgtg tggtccagga tctttttacg cattctgatg ctgtccggcg tgatctcaag
     9661 cagttcatcc tcgccgatat attcgatggc ctgttccagg ctgaaaatgc ggggcggaat
     9721 cagcttgatg gcatcgtctt ttccggcagc gcggttgttg gtgagctttt taccacggca
     9781 gacgttgatc accaggtctt tttccttgct gtgctggcca accaccatgc caccatagac
     9841 ttcggtgttg gggttgataa aaaaggtgcc gcgcgcctga aagttgttga gcgaatagcc
     9901 cagcgcgatc cctgtctcca tcgagaccag ggacccatgg ttggcaccgt tgatgtcgcc
     9961 tttgtaaaag tcgtattcca ggaagaattg gttcatgatg ccggtaccgc gggtctgggt
    10021 gagaaactcg ttgcggcaac cgatcagccc tctggagggg accttgaatt ccagacggga
    10081 ataaccgtcg gtgggaggtg tcatgtggat caattcaccc ctacggccac ccatcatatc
    10141 gatcacggtg cccacaaact cctcggaaac atcgatcatg gccagttcga tcggttcggt
    10201 gatcttgcca tccttctctt taaagagcac ctgaggcttg gagacctgaa actcatagcc
    10261 ttcccgccgc atattttcca tcaggataga gagttgcagc tcacccctgc ctttcacgat
    10321 gaaggtgtcg ctggactctg tcttttccgc gatcagcgag acgttggtct gcagttcttt
    10381 ctgaaggcgt tcccagatct tgttggagct cacccacttg ccgcttctgc ccaggaaggg
    10441 ggaatcgttg atgataaact cgatggcgat ggtgggttcg tcgatgctga tgctgggcag
    10501 ggggacgaga ttgtcttcat gggcgatggt ctcgccaatg tctatctgct ccagtccggc
    10561 gattgtgatg atatctccag cataggcggt cttcacttcc tttttgtggg ttccttcata
    10621 tacgaataga gcgttgacct tgtaggggat aatgctgccg tcgcgtttga tcaacatcac
    10681 ttcctgaccg gttttgacgg tgccgctgtg gatctttccg gtgcccagct tgcccagata
    10741 attgtcatat tcaattgccg aggtcagcat ctggaagggc tgttttctgt cgccggcggt
    10801 gtctggtact tccttgatga tcatgtccag cagcgggaag atatttaggg gttcgtcgtt
    10861 tgggtcatcc accgcgtagc catctttgcc ggaggcataa atcacggcaa aatcaagctg
    10921 ctcatgagtg gcgttgagct ccagaaagag ctcgaagatc atgtccaaca catcgtaggc
    10981 acgggcattg ggcctgtcaa ttttattgat caccacgatc ggctgcaagc ccaggtccag
    11041 agcgtttttg agcacatact tggtttgggg catgggaccc tcaaaggcat ccaccaacaa
    11101 tagtactgaa tccaccatct tcatgatgcg ctgcacttcc ccgccaaaat cggaatgacc
    11161 aggggtatca accagattga tcttgtagcc tttgtactcc agggccgcgc actttgaaaa
    11221 gatcgttatt ccgcgctctt tttcgatgtc gttggagtcc ataacccgct ccaccaggcg
    11281 ttcattgtct ctaaacaccc cggcctgacg caacatcgcg tccaccaggg tggttttgcc
    11341 atgatccaca tgggcgataa tggctatatt cttgattttc cgcattttat tcctttggaa
    11401 ttgtgtgatt tagagcctta caatatggga ggggtgtttt agtcaataga aagtgatgca
    11461 ggagggaaaa cccaagataa cacattgaac attttataca aacccgggct gagttcctcc
    11521 ccgcaaaagc attgacaaaa gcagggcctc caaaattatg gcagaacatc atattttgcg
    11581 tgtaatctat taaaaaataa agcagaactg gagataccaa tgaagacagg cggatgccgt
    11641 tatggaacac atcgcgtaat tgagccgcag ggtgttttgc cccaaccggc cagaattttg
    11701 aacaacgaca tgagcgagat ttgggataat gagctgctca tcgacgtgat tcgtctcaac
    11761 gtggacagcg cgtcttttca ccagatcaaa aacaaactgg tcgcgcaggg ccacacagat
    11821 attgaaaagg ccttcgcgga gcatgccata gacctcacca cccgcaccgg caaacacaaa
    11881 aatgaagaca ccggatcggg tggaatgttg attggccggg tggccgcgat tggcgacaag
    11941 tttgaaatga aagacaagat caaggtgggc gacaaaatcg ccagcctggt gtcgctttcc
    12001 ctcacccccc tcaagatcag caagatcaag caagtgctgc tcgataaaga ccaggtggag
    12061 atcgaaggtc aggctattct cttcagcagc ggtatctatg ccaagctgcc ggacgacatg
    12121 gacgaaaatc tggccctctc agttctggat gtggccggcg ctcccgctca ggtggaaagg
    12181 ctggtgaagc ccggtgacac ggttgtaatt atcggtgcca acggtaaaag cggcatcctc
    12241 tgcaacgccg tggccagaga acgggctgga atatccggca aagtgatcgg cgtggtgcgc
    12301 aatcagagct acgtccccac ctgtctggaa accggttgcc atgaagtgat cattgccagc
    12361 gccaccgatg ccatcaccat ccagaaagaa gtctcaagac tcaccgatgg caagatggcc
    12421 gatgtggtga tcaacgtggt gaatgtggaa gataccgagc tgcccaccat catggcttgc
    12481 aaagatcgcg gcattgtcta ttatttttcg atggccacct ctttcaccaa agcagctttg
    12541 ggcgcggaag ggattggcgc ggatgtggat atgatcctgg gaaacggcta tgcccaccat
    12601 cacgccatga tttctatcga cctgctacgt cgcaaccctg tgctgatgaa actctttaaa
    12661 gaacgttata ccgattgagg gaggatttat gaaccccgcg aagatttctg gcgagcggga
    12721 gctcaccata cctattcgca gtattgccac cgatgcgcag tggcaggact ggcactggca
    12781 gctccgcaac cgcatcacgg attttgacac cctgagcaaa tatatcaagc tgctgccgga
    12841 tgaaaaagcc gtggccgaca gttccgtctt ctcattccgc atggccatca ccccctacta
    12901 tctttcgctg atcgatccct ccaatcccaa tgatccggtg cgcctgcagg ccatccccag
    12961 aatcgcggaa ggcaatatca gccatgtgga tatggccgat cctctgcatg aggatgccga
    13021 tgctccggtg cctggaatga cgcatcgcta tccggacaga gtgctgctgc tgctcaccga
    13081 tcagtgtgcc atgtattgcc gtcattgcac ccgccgcagg aaagccggtg aacatgatgc
    13141 ccccatgccg cgtgaaaacg ttgacaaggc catccagtat atcaaagagc ataaagaagt
    13201 tcgcgatgtg atcctcagcg gtggggatcc cctcactttg ggagatgaac gtctcgatga
    13261 agtgctgggc aagctggctg agatcgacca tgtggatatt gtccgcatcg gtacccgcct
    13321 gcccgtcgtg ctgcctcaaa ggatcacaga cagtctcctg ggcgtgctgc gcaagtataa
    13381 gtttgtgtgg ctcaacactc acttcaacca tcccaacgag ctggcggaag ataacgtgaa
    13441 agccctggcc cgccttgccg aaaccggagt ggtgatgggc aaccagagcg tgttgctgaa
    13501 aggcgtcaac gaccatgtgg acgtgatgaa acaactgctg cacaagctgg tccgcaatcg
    13561 cgtgcgtcct tactatatct atcaatgcga cctttcagag ggcatcgctc atttccggac
    13621 ccccatcgca aaaggcatcg aaatcatgga atccctgcgc ggccacacct ccggcctctg
    13681 cattcccacc tatgtggtgg acgctccagg tggtggtggc aagatccccg tgatgcccaa
    13741 ttacatcata tcccagatgc cgggccgggt gatcctgcgc aattatgaag gctttataac
    13801 ttcctacacc gagcctggat acgaagctca aaaggatgat aactaccgcg aactctgccc
    13861 tgaagaacgc cgctccagtg atggcgtcat gagtctgctg cgaggcaaga aagtctccat
    13921 cggaccggcc gacaccagac ggaatctgcg gcgtaaacaa taatctcgta acgcgtaacg
    13981 cgtcacgggt cacgtttcac aatgagatat aagaccatga gagatgattc aacgcttgag
    14041 ccgcgggtat cgcgtaacgc gtaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnntaagac catgagagat gattcaacgc ttgagccgcg ggtatcgcgt aacgcgtaaa
    14221 gcgtaacgct taacgcctat gagctttgcc tggatttccg acgatggcgt gagagtgatc
    14281 gccatcgtcg gaatctgcaa aaacgccggc aagacttctc ttttgaacca tttgctgagg
    14341 gcctgtagtg ataaaaacgc atacggggac gtttttgaat ccaagatccg cttcggagtt
    14401 ttttccaccg gcatcgatgg tgaggagcgg gatagcgtgt tcaacaccgc caaacctgcc
    14461 gtaaaactcc ctgccaacac acttttttgc tgtgatgcca tggccctgga ctccctgggt
    14521 ggagaggtgg aaattcaggc atcgttatca aacccccaac gccctttgtg gatagcccgc
    14581 agcctctaca gactggatac catgatcacc ggtcccgctt cagtagtgga ccagattgag
    14641 ctcagccgga aaatgcttgc tctgggagcc ggaaaaatcc tggtggatgg ttctttggac
    14701 cgtaaatcca tcgccctcag cccagatgta gatgccgtgg tcctggtatt aggcgcttca
    14761 tatggaaatc cggctaagct ggagacggaa ctgaaacgct tgttgcggtt ggccgcgttg
    14821 ccggttttcg acaccggtcc ggacgagtat aagctgctgc tgggcagcga cgaactgctc
    14881 acgagtgatg ggcgtacctg gcagagcacc agcctgcaaa ccctatccgg caaagaatct
    14941 gaactggata aaatgctcag aaacaacctc agttccagcc gactttatat ccccggggcc
    15001 gtcaccaaca gcagtctgga cgcgctgtta agcctgctgc ccaaaagacc ttacaccctc
    15061 gtcctgcgtc atcccgattg cctgaagctg gatatatcgt ccctgcaaaa gctggctgag
    15121 cgggttgagc taacgacact tattcccttc aggctcaaag gctttgccat caactccgag
    15181 ggagtgggcc tcgccagtga agacgccgcg cgcttcaggt cacggctcag gaccagtttc
    15241 ccccagctgg acctgataga cattatggaa ttgacacatg cttgaaagag ataaacttac
    15301 gaccaccacg gtaaacctgg gactgggggc aaacatcctg ctctcctttg ccaaaacgct
    15361 tgtcggaatc ctgggtcact cgtcagcttt gctggcagac ggcatcaact cgtcttccga
    15421 cgtggtctat tacgttgccg taaagatatt tatgcaccag gcccgcaaac ctgccgacgc
    15481 cgaacaccct tacggacatc gtcagttgga gaccatctcc gccatcgtgg tgggcgcctt
    15541 tatcctcacc accgggatcg ctatcttgtg ggacagcatc aacaaggtct ttgacctgct
    15601 aagtggatac accgtagggc gttccgcttc catactggcc ctctggatag ccatcgggac
    15661 cctggctttg aagctctacc tttaccactt cacgcgcagg accagcagaa ggatcaacaa
    15721 tcccaccatc cgcgccctgg caaacgatca cctgaacgat atcatggcat cccttgcggt
    15781 gatcattggc gtggttttgg gccaattggg atatttctgg atggacccgg cggccggtgc
    15841 aatcgtggcc atctacatca tcaagactgg aatcgggatc atcatggatt cctcgggaga
    15901 gctgatggac agcgtcccag accccgaatt tggtaaactc gtccgttctc tggctttgcg
    15961 ggtggaaggt gtccgccgca ttgaagagct tggcgtgcac cgctttggac cttacttcac
    16021 gatcaatatg accatcggtg tgaatggtcg gatcgccgtt gaggagggcc ataaaatcgc
    16081 ccacagcgtg gaagccgcgc tcctgaacaa cttcgacagc tcccttcgca gtgtccacat
    16141 ccattatcat ccggctgagc tgacagaatc aaaccccatg gagaaatcat gaaccctggc
    16201 agaaatatct tgttgggcct cgtgctgttt gtgctactgg gcttctcagt ttacagcgtt
    16261 ttcgatccgg accggcctca cagagctaag acccatcata tcagcaccgg acaggacagc
    16321 ctcaagaccg atccctggat cgtggaaatc gttccggacg gtggcagcct gtttggtatc
    16381 atggaaaagc atcagatccc cttgagggag atcgccaccg tcacctacca gtttagcgat
    16441 tttatcgacg tctccacgat ccagccaggc gacactctca aattaaaact ctctccctca
    16501 aaggataaga tcgaagcgat gaccttcgtc caggagccca ccattcgtca tcagttcatc
    16561 gtgcagggcg acagcctcgt ctataatgaa gaaaagctgc ccgtacagga agagatcaag
    16621 ctgctggctg gaaagctgga aggtacgctc agcgcctctt tggacagatt gggactgccc
    16681 aaccaggacg cccagcagat catcaacgcc ctggaagcca agatcaattt ccgccgcgac
    16741 gccaaaaccg aagacgagtt tcagatcctg gcccaactgc gctactttga ggggaagctc
    16801 attcctggcg ggaaggttct ctacgctgcc tatcatggaa aaaaggccgg caagcacgag
    16861 ctctttcgct atgaagatcc caatcccaag tctgtactca cgggcctcta cgggccagac
    16921 ggaaagatcg tgaggtccac cagtggaggc ggagtgggtt atcccctggg atccatccac
    16981 gtgagatcca agttcggacg tcgcattgat cccgtttatg ggcactgggc catgcatcag
    17041 ggggtcgatt acaaggcttc atatggcaca ccggtgatgg ctgtggccaa tggcaccgtc
    17101 acggaagccc attacgcggg cggttgggga aaccaggtga ggatcaaaca ccccagtggc
    17161 ctgaccagcc aatatgccca tctttcttct atcgccgtgc gcagaggtca aagcgtcaat
    17221 aaaggtcagg tgatcggcag agtgggcagc accggaaaat ccacaggccc ccatctgcat
    17281 ttcggcttga tgaaaggcgg ccagtggatc aaccccacca atctcaaaat ggttgcctcc
    17341 gaacagctta ccgtgatcca gctcaaggat ttcacccgtc agcgtgacct tattaaagca
    17401 caattgcaac agtatctcaa tcctccggcc cccgtcagtg ctacagtcaa aacctgataa
    17461 atcaggctac gagtatcaaa ggaccggccg ctatttcatc caggtggcgg gttcgctgga
    17521 gcgcctggcc agagcagagc tgctcgatct gggggctaat atccacacgg aagttccccg
    17581 gggatttttt gtaagctgca atctggaaac tctttaccgg attatctata catcccggct
    17641 gtgccagaga gtgctggccc cactggtcag ttttgcctgt cacagcgaaa aatatctggc
    17701 tcgccagagc gcgtccctcc tcgactggac ctcgctgttt gaggtggatt ccagctttgg
    17761 catcgatgtc aacctctctg gcagcaagat caaacattct ctctatgcgg gacaactggt
    17821 caaggacgcc atttgcgatc aatttcgcgc ccaatctggc agaagaccgg actttaggac
    17881 cacgaatccg gacatcaact tcaacctcca catcaaggac aactatgcca ccctcgccct
    17941 ggacctcagc ggcagcatgc acaagcgggg atatcgctca ggcccttctg acgctcctct
    18001 gcaggaaaca cttgccgcgg cgatagttaa gctcagcggt tgggacggcc ggaacccact
    18061 ttcagacccc atgtgcggtt ccggaacgct gttatgcgaa gctctgatga gctggtgcgg
    18121 aatcccagca gctatcttga gggaaaacaa atacatcgag cgtttcccag accacgaccc
    18181 caagctctgg aaagagctgc gcgcccaagc caatcaagcc atgcgtgagc taccggatgg
    18241 attgatcagc ggttctgacg tcagtgcaac ggccctggcg gtggcgagag cttctctgga
    18301 cagactgccc tacggcagag gcgtaaaact ggtcaaatca cgcttccagg agcttccatc
    18361 ccaggcgggg cgctgtgtga tcaccaatcc accctatggc gttcgcctcg aagaaagcgc
    18421 ttccgtggca aagatataca acgatctggg cgactggctg aaacagcgct gtccatcctc
    18481 ggaatcctat atcctctgcg gttcagcgga cctggcatct cagcttcgcc tcagggcctg
    18541 gtggaaaaag tccctcaaaa acggcgatct ggaagtgaag ctggctaaaa tcctgatccg
    18601 ctgaacctgg ctgctcataa gccatagaac agcaaggccc gtgggatttt tccccgttgg
    18661 agttcaagcg gccaggataa agctatatat ggcagcgagt ttgcgggccg cttggacttc
    18721 aaccagcaca ctcgcgagat aaatgtcttc gatgtgctgc ttgaagtcca aagccccgtc
    18781 aagcaag
//
LOCUS       DAOSFN010000036        18463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2458_length_18463_cov_8.855932, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000036 DAOSFN010000000
VERSION     DAOSFN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 18463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18463
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2458_length_18463_cov_8.855932"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..365
                     /locus_tag="PLO35_03810"
     CDS             57..365
                     /locus_tag="PLO35_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOH78822.1"
                     /translation="MKIDLSFSDKVASMKIDGILNSENAHILQEKLSEVLKSESILLE
                     LDLFDCRNISSTGIGKILLFYKDFISKGGEIEVTRSSNSVYELLSMLKINQLFTVNLG
                     "
     gene            362..1282
                     /locus_tag="PLO35_03815"
     CDS             362..1282
                     /locus_tag="PLO35_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH78823.1"
                     /translation="MTQDSAARKILVVDDSVSIVFSIGKILELNGYAVDSAYNGSDAL
                     RKISQQVFDVVVCDIEMPGISGMNFLEKVRDEYDHDLDVILMTGYLDQDYFIQAIRLG
                     ASDFIRKPIESKQLLHSITRILERRRNKNDISNFFQCLYGSRMVFEIDPMRFWQAGIG
                     KTFNQFLRQSLNLKRNFANELLVCIDEMIYNALIHGTLSLSKEQRHLSHDQLRKLVNG
                     RLEEGLITDKSIRIGLDLNLKDGFISISVEDDGEGFDHESWLRRLQSEQEINLDAHGR
                     GISMLYHLSDHLEFDKNGRKVTITRALPKD"
     gene            1295..>1938
                     /gene="prmC"
                     /locus_tag="PLO35_03820"
     CDS             1295..>1938
                     /gene="prmC"
                     /locus_tag="PLO35_03820"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOH78824.1"
                     /translation="MKLANLFQDAMSAIKANNIPESALRLLLADTFGMGLAELNTRLN
                     MDLKDDILTRWNLRLERLLRGEPPQYISGQACFYGLQFKVCPGVLIPRPETEGLVELV
                     LERLHEHQLVLDCCCGSGAIALAIKHMRSDATVHASDLSSEAVTLAKQNAADLMLDIS
                     IYECDLFPTTTPGYDLIVCNPPYISDTEYQRLDPSVRDHEPALALRSGNDGLDH"
     assembly_gap    1939..2038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2041..3294
                     /gene="murA"
                     /locus_tag="PLO35_03825"
     CDS             2041..3294
                     /gene="murA"
                     /locus_tag="PLO35_03825"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOH78825.1"
                     /translation="MDRFIINGCRDLSGSITVSGAKNAILPIMAAALLAPGKSMLRNV
                     PNLIDLKTMAHLLRVLGARVEYEHGSMAIDSRDLCYNEAPYELVSKMRASVYVLGPLL
                     ARLKEARVSFPGGCAIGTRPVDLHLKAMEALGATISIEHGYIHAVADQLIGADIHFEK
                     SSVGATINALMAAVLAKGQTRMFNAAMEPEVDSTIDMLNKMGAHIEGKGSTTLCIQGV
                     ENLYPVEMSMIPDRIEAGTFLIAGALSTSPVTVTNCEPAHLTVLIDKLRATGCELAID
                     GSSITVTPPAKIKAVDILTLPHPGFPTDLQAQFTVLMTLAEGVSYVEDTIFPDRFMHV
                     AELNRLQANIRMERNVACVKGVKELSGAEVMATDLRASAALVLAGMVARGDTIVSRIY
                     HIDRGYDRIEEKLNGIGARITRENA"
     gene            3287..4252
                     /locus_tag="PLO35_03830"
     CDS             3287..4252
                     /locus_tag="PLO35_03830"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOH78826.1"
                     /translation="MPELILVTGGAGFIGSHLCEALLRDGKRVLCVDNFCDFYDPARK
                     HANLRGCLGNPLFALETLDVTDAMGLADVFDKYNITAVCHLAAMAGVRPSIQNPGLYL
                     HVNLSGTLNLLKLCQKHGIKSFVFASSSSVYGNGPKLPYSESDPVDHPISPYAASKKA
                     GELLCHTWHHLWGISILCLRFFTVFGPRQRPDLAIHKFATLLKAGRPLPVYGDGSSSR
                     DYTYIDDTVSGIMTALELTGKTSVYDVINLGNDQSVSLEHMVHYLERVSGIKALRQQL
                     PPQDGDVLHTRADISKAARLLGYNPQTSFEQGLEHFWRWFDQQSE"
     gene            4574..7759
                     /locus_tag="PLO35_03835"
     CDS             4574..7759
                     /locus_tag="PLO35_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH78827.1"
                     /translation="MRIAKRPEPTRTESLDLLMQAVVSIYLYMARSARLSGSEINVID
                     RMLRYLFGNEIPLYQIEHARLHTLPLREAANLLNRHLSHADKLKLILNLTALAYRDPN
                     KLHILGNVEIVELTDLLRVDVNILDAFYDVAEGLAASLALPVSSFLGSRHHILKGSML
                     WGAESADFPLTITSGEPLIFVMIENLVLVCHTASGQSLQSGCWLENRSDGSRTELTSG
                     LFYHFKASQQLVIRDDAKSLEIDLEDVWSIYRLPASGCQIPLRSENGDTRKLCWEAGR
                     LWLAPLKASSSSRNQKELALNDSLPGFQTSEPVLRLITRRGTALRQIATASELFVQRL
                     DGRHRLIHSRDGDCPLRLVRETEQWWLEALSSDPVFVNRTIVTQRVAFAFNNDVLTLD
                     NLSYLINRDLDLIEIPIEIRELRINDVYHEFSRDKTVALDGISFTLNQGSMMAIMGPS
                     GSGKTTLLKVLLGELEPQRASIEIDQMDFRKNYGFFRKFIGYVPQDDLLFANLSVYEN
                     VYFNLRLRLPQIKDPAQIKARIDNLLRRVGLFEQRDMIVGDVMNKRLSGGQRRRLNIA
                     LELVSNPMIIILDEPTSGLSSKDSENIAGFLAELKEQGKIILCTIHQPNATILKQFDR
                     VLLMDVGGTQVFNGDTESVFTYFDDELASSGTESQKLAEKKRLRMPEYFYDLIELREN
                     DHERRFQAQHWKQKWRDHRFRQALDLRSGNEAILEDSRLGVAKESRGSGLSNLALLLR
                     RAFINKSRSRLNLIMTLGVAPLLALLTSLILRSATGTEVYSFRENQNFPLFCFIQVII
                     FVFIGLANSVDDVLGEKRVILREKNLNIRVLPMIASKNVVLFAMTLVQSLLFYQISAW
                     VLGIRGSFWPLLLSLQLSGMLGYKMGLLSSSLINDRAAIINILPLILIPQIMFSGAVI
                     RFQDMNSALRIDKKREVPEFCQVIPSRWLFETSLLAQDKLNSRSRAFTHYKKLLSDMR
                     DPGYLDYVQAFDDCLLKRPESSHSNRVAQSLIREAHGRHLEEGRNVFLSHTSQCGSKS
                     LATLWIDLAVLMVMIMFLDLVLWLKLRFWYR"
     gene            7764..8189
                     /locus_tag="PLO35_03840"
     CDS             7764..8189
                     /locus_tag="PLO35_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOH78828.1"
                     /translation="MNIYIRSQDRDLCKYLDDRALGDLNRFVREAGYPIGAVICKQGE
                     RIDSFLVVIEGELALVSTTGKTLGHVYTGEMVNELHYLKDHPAPWEIRAVRPTRVAVI
                     SFTDMNSFVDKDPERAARIQAAINDSLCLKNIRLTHLEN"
     gene            8191..8619
                     /locus_tag="PLO35_03845"
     CDS             8191..8619
                     /locus_tag="PLO35_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOH78829.1"
                     /translation="MEHPLLRYLSDSEVAMIKAIAREVDFEAGDLLIQTGERSRDLVC
                     IDSGSVAVMVQTPDGQVKQVSELGSGSLIGEMNFVIPSRRTADVVAVTPTRITVFPYL
                     GLTELLKGHPVLAAKIFQAINEALATKYLNMLAKTNDSGE"
     gene            8788..9111
                     /locus_tag="PLO35_03850"
     CDS             8788..9111
                     /locus_tag="PLO35_03850"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOH78830.1"
                     /translation="MNEKFTKLKDLLKEGLTGYPVKKTILFGSQVNGNSDEQSDFDIL
                     ILVDDSLQTAAYNELKFSIHKLLIDSSFKYPVDLIVKHESAFETERHVFGALAYRVCR
                     EGIVL"
     gene            9108..9497
                     /locus_tag="PLO35_03855"
     CDS             9108..9497
                     /locus_tag="PLO35_03855"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOH78831.1"
                     /translation="MSKDYCTILIGKGDSDFKTAMLLMKADPSLHETAGFHIQQAIEK
                     YLKAYLISQHIEPPRSHNLFYLLNLCIDLDQSFSLFVDPVLSTINDFAVTFRYDVMPE
                     SDTLDLEHALHFTTRIRDLVMGLIPSA"
     gene            9568..10650
                     /locus_tag="PLO35_03860"
     CDS             9568..10650
                     /locus_tag="PLO35_03860"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOH78832.1"
                     /translation="MYYLPTRIIFGEHALPSSQAQIAGLGSKALIVTGKSSAARSGAL
                     DELLNFLAVQNPVIYDGINENPHLESVVMGADLMREQDCDYVIGIGGGSPLDAAKAIS
                     LAAANKLGIDDIYRTDLFKRAFPIVAVPTTSGTGSEATQYAVLTDSRIGKKAGWGHAL
                     AFPALAIVDPRYTLSLSYHVTLNTAIDALSHLLEGLYSRLREPLIFPLIHKGIELIVK
                     HLPIALNDPQNLEARTALSQASLYGGMTIAQGSTTLQHSIGYPLTTAFNVPHGLANGI
                     VMKQMMELYWPEVDGLIDSAFGSFDYNLQQFYDWLDKLELRAEMRIDEDFIASKVPEV
                     MASRNMANNPVPAGPDDVAKIYRSLM"
     gene            10650..10988
                     /locus_tag="PLO35_03865"
     CDS             10650..10988
                     /locus_tag="PLO35_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOH78833.1"
                     /translation="MKRTEFDAERERLNKLVLDNANLNTKRFFGLDHASYQDGALPVK
                     TKELLGLIASLVLRCDDCVRYHLGRCFDEGTSDEELREALQIGLIVGGSIVIPHLRRA
                     EEYWQSLTTS"
     gene            complement(10975..11304)
                     /locus_tag="PLO35_03870"
     CDS             complement(10975..11304)
                     /locus_tag="PLO35_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOH78834.1"
                     /translation="MKTILLLSISNIFMTFAWYGHLKYKDSPLFKVILISWLIAFFEY
                     CFQVPANRIGHGTFNAFQLKTIQEVITLVVFCAFSVLYLKENLRWNYIVGFGFIVLAV
                     VFIFKKW"
     gene            complement(11301..12170)
                     /locus_tag="PLO35_03875"
     CDS             complement(11301..12170)
                     /locus_tag="PLO35_03875"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF005491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOH78835.1"
                     /translation="MDYISKRVLAAHDLSGFGHTSLLAAIAILYRMGIRVAALPTAML
                     SANTDHEGFSFRPFDDGMKDFIAHWQELGLRFDAAYTGFLGSPDQVDSIIEAIKTLVD
                     PKGLVLVDPVLGDQGKLYSCFDLSMVKAMKKLIKHADLITPNATEAAILLNEDLLEDP
                     DVEWDAIACRLSRLGPSKVVITSIPTRLPDIRLCGVYDKSGDRWDEVIFPVNKGTHPG
                     IGDCFASFLLAGMLGKHSLKQSVRGAAGLINFALQIEEPKGYSWREGLQLEKLLALDY
                     DLDSFYRSSRRKK"
     gene            12335..12898
                     /locus_tag="PLO35_03880"
     CDS             12335..12898
                     /locus_tag="PLO35_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOH78836.1"
                     /translation="MNTSFDVHAIYQLMHDSHIQFSYKGPITQDVLISLGDTIKEELH
                     RDDCDAKVIRRVFSVLVEAAHNILKYSSEKTVLDKSKTVGIGLIGIGKTPEGCFVVFS
                     GNFVSPTEAEDIKTRLDYINTLSPSELTRNYQQQMRQGTISANGSAGLGLYEVARKSG
                     RPIDYSFTPGPDGMMFFEMKIVVEVED"
     gene            12901..13281
                     /locus_tag="PLO35_03885"
     CDS             12901..13281
                     /locus_tag="PLO35_03885"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOH78837.1"
                     /translation="MKKLHIEGTRYSPRIELDPETRTLLIEGDSYPENAVDVYGPLIT
                     ALDEYFEQPNTPLNIKMLSFFQNTSSSKMITDMLAKLQRFFDQGHPVELTWFYPSDDL
                     EIKDTFELFLEDASFPHSILQLEE"
     gene            13288..14286
                     /locus_tag="PLO35_03890"
     CDS             13288..14286
                     /locus_tag="PLO35_03890"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOH78838.1"
                     /translation="MGQNDLFGKEQELLDRAKALLESHKSEGCVTIEDFQALATAYSE
                     LLKQSSQLTRLSDKQQKRMNTLMDRLSRYVPPPIYKKITSGKEKVETGTTRRVKLSIF
                     FSDIKGFSWHSTKMEGEALTAILNSYLEEMTHIIFKHGGTLDKYIGDAIVVFFGDPDF
                     SSDAEHARRAVMMALEMRQRMTELQHKWFELGFPEPLHIRMGISTGFVNVGNFGSSEH
                     MDYTVIGAPVNMAARLQAEASEDQILISHETWSFVKDVLDCTEGRYYDLKGFYNPVLA
                     YEVKGLKLQSPERQRVIEDDSSGIYLRYDPQKLSRDTLLDIIHKLENDLSGDPSIE"
     gene            14311..>14498
                     /locus_tag="PLO35_03895"
     CDS             14311..>14498
                     /locus_tag="PLO35_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78839.1"
                     /translation="MRPKLSSIIVLICIVLLPLLTGASPEVQKAIEEYQRVQNTNRRV
                     RIFPFDEGNPSYLGYSFT"
     assembly_gap    14499..14598
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14609..18445
                     /locus_tag="PLO35_03900"
     CDS             14609..18445
                     /locus_tag="PLO35_03900"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOH78840.1"
                     /translation="MFEGYPDVERDSLLLGSNAQTYDGSIYVAGIHYLYKWQGHAWKR
                     YAFTKNDAIQFVKACGNKVLCIGKTSYCVLTGNSWTSYKAESWLLDGLDKQRFMITGM
                     DEWEPQYFSAVTLLPENQLVVCNIQGEYNLKTLAKRCKDDGVPVFINIGSNGKSDSTI
                     VLSSELKKQQIADVNLIEPQFHHGTGSDIWLYLRHTPYFYYFDSKTRSFKPSWYPEGT
                     EISGIMRFKEPLTISWKTKTLSIRELIIYTQNGKVVAESLGPVDTEGRFRIEGTLPDA
                     VPGMKYQFIISIDDTAYSSSYAQYPPDKQVQIAYHYDSKQNKLIMRDLSSLRSNVHNQ
                     LPFPDLGLTNSKTFVYNKRSITEGNIPGIHAFNALGLKDNERYDFSITDPGKRSEFSY
                     LDPVAKTALITTQYSHYLVPLAKPVKPLNAFDISQYSELETHRWPQNSFISSGMYSQF
                     LLRSRDIRPGQEADIHNIDGQSVTPLVREKWLEWSHNGANSFILVGRVEGSRKHIFEW
                     NLNSNRKLPITSTDKNTRIIQSTDGAGVILINDQDLGFQLWHKADPNSDFKLTSSGSF
                     DAAAKVLDTKLKSDQGWGYKILSFLKNGQTILLSDGKLACLPPLDLTMASDSSISYGE
                     RYYPSLDPKDRPREFTASKNNNEVSINVVPFIFNPVSGDLEFKPDWIEIYQLGSKTLV
                     SYYEMDDKNQYYYHSSVLSGWKADKSPNDFRILCANGKDPVRQRLADGDYVLYETNDG
                     KLYYCTQNGWNYLDIKGYTGDYGPLLEVISVSPMMTRSSHQISTLQPSTIARSTGPAM
                     YADDAVLALRFERGIVKYPLSGEVVSVITNTDGIEFNNRTGMVFDWQHQRLLVADDYG
                     IYEISSPSSSAKLHIPWLENNGNRIPRSDFIRIKYKQRDLRIPINILQSPDPRRFQIK
                     YQLIGYDKEARLREWSPYLEYNRLPAGKYTLSVIAYSPDGVPTDELKVPIRVLPPLWD
                     TWWARLIYAIAIFFSLRAIMRWRLKRLERRNKMLEQAVSERTIELSEKQRRMTESIQY
                     ASLIQRSILPQTAQLNELFSSHFVIWKPRDIVGGDFYWLHHKPESPRWLFAVIDCTGH
                     GVPGALITMTVNSILNHLVTDQGIEAPVTLLKELHSQLGAALHQEEDRTQQDGLEISM
                     IAVDKSKSELEFAGAGLHLIHWHPELEGPELIRGYKHGLGGLKRYKQLDLSSSVVSYR
                     QDSIFYMYTDGIIDQPRTNDERRIRFGHPQWLEFINRVAVYDLMRQQERFEGKLESLL
                     EIHEQRDDITVVGMRM"
BASE COUNT         4650 a         4781 c         4606 g         4226 t
ORIGIN      
        1 tgtttggcgt aacgcttagc caataacaaa cagaccaaat attaaaggag taagcgatga
       61 aaatcgatct aagcttcagc gataaggtag cttcgatgaa gatcgacggt attcttaaca
      121 gcgaaaatgc ccatattctg caagaaaaac tcagcgaagt gctcaaatcc gagtccattc
      181 tgttggagct cgacctcttt gactgtcgca atatcagcag cactggcatc ggcaaaattt
      241 tattgtttta caaagacttt atcagcaagg gcggtgagat cgaggtaacc cgcagttcca
      301 acagcgtcta tgaactgctc tcgatgctca agatcaacca gctcttcacc gtcaatttgg
      361 gatgacacag gattcggcag cccgcaagat tctggtggtt gacgacagcg tcagcatcgt
      421 cttcagtatt ggcaagatct tagagctcaa cggctatgcc gttgattcag cctacaacgg
      481 cagcgacgct cttcgcaaga tcagtcagca ggtgtttgac gtagtggtct gcgacatcga
      541 aatgcctggc atctcaggga tgaatttcct ggaaaaagtg cgcgacgaat atgaccacga
      601 cctggacgtg attctgatga caggttatct ggatcaggat tactttatcc aggcgatccg
      661 tctgggagcg tcagacttta tccgcaaacc cattgaatcc aagcagttgc tgcactccat
      721 cacccgtatt ctggagcgtc gtcgcaacaa gaacgacatc agcaactttt tccagtgcct
      781 gtatggctcc cggatggtct tcgagatcga tcccatgcgt ttctggcagg ctggtatcgg
      841 caagaccttc aatcaattcc tgcgccagag cctcaatctg aaacgcaatt ttgccaacga
      901 gctgctcgtc tgtatcgatg aaatgatcta caacgccctg atccacggta ccctcagcct
      961 cagcaaagag cagcgccatc tgagccacga tcagctgcgc aagctggtca atggccgttt
     1021 ggaagaggga ctgatcaccg acaaaagcat ccggatcggg ctggacctca acctgaaaga
     1081 tggattcatc agtatcagcg tggaggatga cggcgagggc tttgatcacg aatcctggct
     1141 gcgacggctg caatccgagc aggaaatcaa cctcgacgct catggccggg gaatttccat
     1201 gctctatcat ctcagcgatc acctcgaatt tgacaagaac ggccgtaaag tcaccatcac
     1261 cagggccctt cccaaggatt gatcccggac ttgcatgaaa ctggcaaatc tctttcagga
     1321 cgccatgtcc gcgattaaag ctaacaacat accggagtca gctttacgcc tgcttttggc
     1381 cgacactttc gggatgggtt tagctgagct gaacacccgg ctgaacatgg acctgaagga
     1441 tgacatcctc acgcggtgga atctaaggct ggagaggctt ttgaggggcg aaccgcctca
     1501 gtacatcagc ggccaggctt gtttttacgg cctgcagttt aaagtgtgcc ccggagtgtt
     1561 gatcccacga cctgagaccg agggtctggt ggaactggtc ctggaacggc ttcacgagca
     1621 tcagctggtg ctggattgct gctgcggcag cggagctatc gctctggcaa tcaaacatat
     1681 gcggtcagac gccacggtcc atgccagcga tctcagctcc gaggcggtga ccctggcaaa
     1741 acaaaatgcc gctgacctga tgctggatat cagcatctat gagtgtgacc tcttccccac
     1801 tacgactcca ggctacgatc tgatcgtttg caacccgcca tatatatctg atacggaata
     1861 ccagcgcctg gacccgtcag tccgagatca cgaaccggcc ctggcattgc gctctggcaa
     1921 tgatggactg gaccatatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     2041 atggatagat ttatcatcaa cggctgccgg gatttgagtg gcagcatcac cgtcagcgga
     2101 gccaaaaacg ccatcctgcc aatcatggcc gctgccctgc tggcgcctgg aaaatccatg
     2161 ttgcgcaatg tgccaaacct cattgatctc aagacgatgg cccacctcct gagagttctg
     2221 ggcgccagag tggaatacga acacggcagc atggccatcg acagccggga cctctgttac
     2281 aatgaagctc cctacgagct ggtgagcaag atgcgtgcct cggtgtatgt gctggggcct
     2341 ctgctggcca ggctcaaaga ggccagagtc tcgtttccgg gaggctgcgc gatcggcacc
     2401 aggcctgtag acctgcacct caaggccatg gaagctctgg gggccacgat cagcatcgaa
     2461 catggctata ttcatgccgt cgcggatcag ctcatcgggg ccgatataca tttcgaaaaa
     2521 tccagcgtgg gggccaccat caatgcccta atggcagccg tgctggccaa aggacagacc
     2581 cggatgttca atgccgccat ggaaccggaa gtggattcca ccatcgatat gctcaacaag
     2641 atgggcgctc atatcgaggg aaagggcagc accactctct gcatccaggg agtggaaaac
     2701 ctttacccgg tggagatgag catgatcccg gaccgcatcg aagccggaac tttcctgatc
     2761 gccggagctt tgagcaccag tcccgttacc gtcacaaact gcgagccggc ccacctcacc
     2821 gtcctgatcg acaagctcag ggccaccggt tgtgaactgg ccatcgatgg ttcttctatt
     2881 acagtcaccc cgcccgcgaa aatcaaagcg gtggacatcc tcactctccc ccatcccggt
     2941 ttccctaccg atttgcaagc tcagttcaca gtgctgatga cccttgccga gggtgtcagc
     3001 tatgtggagg acaccatttt cccggatcgc ttcatgcacg tggcggagct caaccggctc
     3061 caggcaaata tccgcatgga acgcaacgtg gcctgcgtga aaggagtgaa agaactcagc
     3121 ggcgcggaag tgatggccac agacctcagg gccagcgcgg cgctcgtgct ggcaggaatg
     3181 gtagccaggg gtgataccat cgtttcccgc atctaccaca tcgatcgcgg ctatgatcgc
     3241 attgaggaga agctcaacgg tatcggggca cgcatcacca gggaaaatgc ctgagcttat
     3301 attagtaacc ggtggggcag ggtttatcgg ctcccatctc tgcgaagcct tgctcagaga
     3361 tggaaagcgg gtgctctgtg tagataattt ttgcgatttc tatgatcccg ccagaaaaca
     3421 cgccaacctc agaggctgcc tcggcaaccc cttgtttgcg ttggaaacat tggatgtcac
     3481 ggacgccatg ggtttggcag atgtctttga taaatataac attactgctg tttgtcacct
     3541 ggccgccatg gccggagtga gaccttccat ccagaatccc gggctctatc tccacgtcaa
     3601 tctgagcggt accctgaacc tcctgaagct ttgccagaag catggtatca agagctttgt
     3661 gtttgcctcc tcctcctccg tatatggaaa tggccccaag cttccatact ccgaatccga
     3721 tcccgtagac catcccatct cgccctacgc ggcttccaaa aaagccggag agctgctctg
     3781 ccatacctgg catcatctat ggggaatatc catcctctgc ctcaggttct tcaccgtgtt
     3841 cggtcctcgc cagagacctg atctggccat ccacaaattt gccaccctgc ttaaggccgg
     3901 gaggccgctt cccgtgtatg gcgatgggtc cagcagccgc gactatacct atatagacga
     3961 taccgtttcc ggcattatga ccgctctgga actcacaggt aaaacctctg tctatgacgt
     4021 gatcaacctg ggcaacgacc agtctgtatc gctggaacat atggtccatt atctcgaaag
     4081 ggtgagtgga atcaaagccc tcaggcaaca attgccccct caggatggag acgtactgca
     4141 tacccgggcc gatatttcca aggctgccag gctcctgggc tataatcccc agaccagttt
     4201 tgaacagggg ttggagcatt tttggcgctg gtttgaccag cagtctgaat agtgttgctt
     4261 ccttagcatc cgagcttcta taccactgtg attcatggtc cttgcttatt cagccgccgt
     4321 agagatatcg tgcccccgga ggcgattttc gccggaggcc ccacggtatc tccacggcat
     4381 ctaagcggca aggatgcaga aattctgctt ctggcccaga tcatcggagc gctgagttgc
     4441 tggcgcgaaa taaatcctgc ctggctgctc tgcaggcctt ggggatgcaa caaaaatctt
     4501 gacgaaatcc atcgcgtcag aataggggtg aaagacggaa agatcgtaaa cccggtataa
     4561 caaggagacg acattgagga tcgctaaaag gcctgagccc accaggacag agtctttgga
     4621 cctgttgatg caggcggtcg tctctatcta tctatacatg gctcgcagcg ccagactgag
     4681 cggttccgag atcaacgtga tcgaccgcat gctgcgatac ctgtttggca acgagattcc
     4741 tctctatcag atcgagcatg ccaggctgca taccttgccc ctgcgggaag ctgccaatct
     4801 gctcaatcgc cacctcagtc atgccgacaa gctaaaactg atcctcaacc tcaccgctct
     4861 tgcctataga gatcccaaca agctgcacat tctgggcaat gttgaaatag tggaactcac
     4921 tgacctgctg agagttgacg tgaacatcct ggatgctttc tacgatgtgg cggaagggct
     4981 ggccgccagc cttgccttgc cagtcagcag ttttttgggc agccggcatc atatcttgaa
     5041 gggctcgatg ctatggggcg cggaaagcgc tgattttccg ctgacaatca caagcggcga
     5101 acctctgatc tttgtcatga tagagaatct ggtgctggtc tgtcacactg cctcaggaca
     5161 atccctccag agcggttgct ggctggaaaa ccgctccgat ggctcgcgaa ctgaactgac
     5221 atcgggcctg ttttatcact tcaaggccag ccaacagctt gtgattcgag atgatgccaa
     5281 gagcttggaa atcgatctgg aagacgtctg gagcatatac aggcttcctg cctctggatg
     5341 tcagattccc ctccggtctg aaaacgggga cacccgcaaa ctctgttggg aagcgggaag
     5401 gctttggctg gctcctttga aggccagttc aagctcccga aaccagaaag agctcgccct
     5461 caatgacagc ctgcctggat tccagaccag tgaacccgtt ctgagactga tcaccagacg
     5521 cggaactgcc ctgcgccaaa tcgcaactgc atcggagctc tttgtccagc gtctggatgg
     5581 caggcatcgg ctgatccatt ccagggatgg cgattgtcca ctccgtctgg tccgcgaaac
     5641 cgagcagtgg tggctggaag cgctgagttc cgacccggtt ttcgtcaacc ggaccatcgt
     5701 gacgcaacgg gtggcctttg cttttaacaa cgacgtcctc accctcgaca atctcagcta
     5761 tctgatcaac cgcgatctgg acctcattga gatccccatc gaaatccgcg aactcaggat
     5821 caacgatgtc tatcacgagt tttccaggga caaaaccgta gctctggacg ggatttcctt
     5881 taccctcaat cagggtagca tgatggccat aatgggaccc agcggcagcg gtaaaaccac
     5941 cctgctgaag gtccttttgg gagaactcga accccaacgg gcctctatcg agatcgatca
     6001 gatggacttt cgcaagaact atgggttttt ccgcaaattc atcggttacg tgcctcagga
     6061 cgatctgctc tttgccaacc tgagcgtgta tgaaaacgtc tatttcaacc tgaggctgcg
     6121 gctccctcag atcaaggacc cggctcagat caaggcaagg atagacaatc ttttgaggcg
     6181 ggtggggctc tttgagcaac gggacatgat cgtgggggac gtgatgaaca agaggctgag
     6241 cggcggtcag cgaagaaggc tgaatatcgc tctggaactg gtctcaaacc ctatgatcat
     6301 catcctggac gagcctacca gcggtctttc atccaaagat tccgaaaaca tcgcggggtt
     6361 tctggcagag ctgaaagaac agggcaagat catcctctgc accatccacc agcccaatgc
     6421 caccattctg aagcagtttg accgcgtttt gctgatggat gtgggtggca ctcaagtatt
     6481 taacggcgac acggaatctg tcttcactta ctttgacgat gaattggcca gcagcggaac
     6541 agaatcccaa aaactcgcgg aaaagaagcg gctcagaatg cctgagtatt tttatgacct
     6601 gatcgaactg cgtgaaaacg accatgagcg ccgtttccaa gctcagcact ggaaacagaa
     6661 atggcgcgac catcgctttc gtcaggccct ggacctaagg tccggaaatg aggccatcct
     6721 ggaagattcc aggctgggag ttgccaaaga aagccggggt agcggattga gcaatctggc
     6781 tttgctgctg aggcgggctt tcatcaacaa gagccgcagc cgcctcaacc tgatcatgac
     6841 tttgggcgtg gcaccgctac tggccctgct cacctctctg atcctgagaa gcgccaccgg
     6901 gacagaggtt tatagcttta gagaaaatca aaactttccg ctcttctgct ttatccaggt
     6961 aatcatcttt gtctttattg gtcttgccaa cagcgtggat gacgttctgg gcgagaaacg
     7021 cgtgatcctg agggagaaga acctcaatat cagggtgctg cccatgatcg ccagcaagaa
     7081 tgtggtgctg tttgcgatga ctctggtcca gagcctgttg ttttatcaaa tcagcgcctg
     7141 ggtacttggg atccggggct ccttctggcc gctgctgctg agcttgcagt tgagtgggat
     7201 gctgggctac aagatggggc tgctgtcttc atcgctgatc aatgaccgcg ccgccatcat
     7261 caacatcctg cccctcattc tcatccctca aatcatgttt agcggggcag ttatccggtt
     7321 tcaggacatg aattccgccc tgagaataga caagaaacgc gaagtacccg aattttgcca
     7381 ggttatcccc tcccgctggc tctttgagac cagtctgctc gcccaggata agctcaattc
     7441 ccgttcccgg gccttcactc actacaagaa actgctttcc gacatgcgtg acccagggta
     7501 tcttgactat gtgcaggcgt ttgacgattg tctgctcaag cgtcctgaat catctcacag
     7561 caaccgtgtg gcccaaagcc tcatccgcga agcccacggc cgacatctgg aagaagggcg
     7621 caacgtcttc ctcagccata cttcccaatg cgggtccaag tcattggcca ccttatggat
     7681 agacctggct gtactgatgg tcatgatcat gtttctcgat ctggttttat ggttgaaact
     7741 gcggttctgg tacaggtgag cgcgtgaata tctatatccg atctcaggac agagacttgt
     7801 gcaagtatct ggatgaccgc gcgctgggtg atcttaaccg ctttgtgaga gaggccgggt
     7861 atcccattgg agcggtcatc tgcaagcagg gagagcggat agacagtttt ctggtggtga
     7921 tagaagggga gctggccctc gtttcgacaa ctggaaagac cctgggccac gtctatactg
     7981 gagagatggt caatgaacta cactatctca aagaccatcc cgcgccttgg gaaatcagag
     8041 ctgtgcgccc cactcgcgtg gcagtgatct cctttacgga tatgaacagc tttgttgaca
     8101 aggacccgga acgcgctgcc aggatccaag ctgccatcaa cgacagcctt tgcctcaaaa
     8161 atatccgtct cacccacctg gagaattgat atggaacatc ctctgctgcg ttatctgagc
     8221 gattctgaag ttgccatgat caaagccatc gccagggaag tggatttcga ggcgggagat
     8281 ctgctgatcc agactggaga acgcagccgg gacctcgtct gcatcgatag tggctcagtc
     8341 gcagtaatgg tccagacccc ggatggccag gtgaagcagg tgagtgaact gggttccgga
     8401 agtctgatcg gcgagatgaa ctttgtgatt cccagccgtc gcaccgccga tgtggtagct
     8461 gtaactccca cccgcatcac ggtttttcca tatctgggac tcactgaact gctcaagggc
     8521 catcccgtcc tggcagccaa gattttccag gcgatcaatg aggccctggc gaccaagtat
     8581 ttgaatatgc tggcgaagac caatgatagt ggagaatgat aagttgaatc gagttttggc
     8641 aagagttcag tcactagttc ttcagcgcat ttgcaggatt tgatcaacga atcacgatga
     8701 ttaagcttga cagctgagtt catcttcaat agcttgccaa ccagccaaaa catgcattgc
     8761 aggcacaaaa ggaaggtcac actggttatg aatgagaagt ttactaagct aaaagacttg
     8821 ctaaaagaag gcttaacagg atatcctgtc aaaaagacga tcttgtttgg ttctcaggta
     8881 aatggcaatt cagatgaaca aagtgatttt gatatcctta tcctggtaga tgacagtctg
     8941 cagaccgcag catataatga gcttaagttt tctatccaca aattgctaat agactcgagc
     9001 tttaagtatc cggtagatct gatcgtcaaa catgaatccg cctttgaaac agaacgccat
     9061 gtcttcgggg ctttggcata cagagtttgc agagagggca tcgtgctatg agcaaggatt
     9121 actgcaccat acttatcggc aagggggata gcgatttcaa aacagctatg ctactgatga
     9181 aagctgatcc ttctctgcat gaaacagcgg gatttcatat ccagcaggct atagagaaat
     9241 atctgaaagc atatctaatc tcgcagcaca ttgagccgcc ccgttcgcat aacctcttct
     9301 atttgcttaa tctatgtatt gacttagacc aatctttttc attgttcgta gatcccgtgc
     9361 tatccacgat caacgatttt gccgtaactt tcaggtatga tgttatgcct gaatctgata
     9421 cgcttgatct ggagcacgcg ttgcatttca cgacaaggat cagagactta gttatgggac
     9481 tgattccctc agcgtgagct tcattttcct ggcaactaac aggcaccggg tttaaaccta
     9541 ctaacactct aatcccaagg ttaatccatg tattatctcc ccactcgaat aatctttggt
     9601 gagcacgcgc tcccatccag ccaggcccag attgctggcc tgggttccaa agctttgatt
     9661 gtgacaggaa aaagcagtgc cgcgcgcagc ggagctctgg atgaactgct caacttcctg
     9721 gccgtccaga atcctgtcat ctatgacggc atcaatgaaa acccccatct ggaaagcgtt
     9781 gtcatgggag ctgatctgat gcgggagcag gattgtgact acgtgatcgg catcggtgga
     9841 ggcagtcccc tggacgctgc caaagcgata tctctggccg cggccaacaa gctgggaatt
     9901 gatgacattt atcgtactga tctgtttaaa cgggccttcc cgatcgtggc tgtccccacc
     9961 acttcaggaa ccggatcgga ggctacgcag tatgcggtgc tcacggatag caggatcggc
    10021 aaaaaagccg gttggggcca tgctttggcc tttccagcgc tggctatcgt ggatccccgc
    10081 tacacgctca gcctctcata tcacgtcacc ctcaacaccg cgatcgatgc tctcagccat
    10141 ctattggaag ggctttacag ccgcctgcgg gaaccattga tctttcccct cattcacaaa
    10201 gggatagagc tgatcgtcaa acatttgcct atcgctctaa acgaccctca aaacctggaa
    10261 gccagaaccg cgctctctca ggcctccctg tatggaggta tgactatcgc gcagggcagc
    10321 accactttac agcactccat cgggtatcct ttaaccaccg ctttcaatgt tcctcacggc
    10381 ttggccaacg ggatcgtcat gaaacagatg atggagctct actggcctga ggtggacggg
    10441 ctgattgata gcgctttcgg gagctttgac tataacctcc agcagtttta tgactggctg
    10501 gacaagcttg aattgcgggc tgagatgcgg attgacgagg attttatcgc cagcaaagtt
    10561 ccggaagtga tggccagccg caacatggcc aacaaccccg ttccggctgg cccggatgat
    10621 gtggccaaaa tctacaggag cctgatgtga tgaaacggac agaattcgat gctgaaagag
    10681 agcgcctgaa caaattggtg ctggataatg ccaacctcaa caccaaacgc tttttcggcc
    10741 tggaccatgc cagctatcag gatggagcac tgcctgtgaa gaccaaagaa ctgctgggac
    10801 tgatagcctc actggtattg agatgtgacg actgtgtgcg ctatcatctg ggacgctgtt
    10861 ttgatgaagg taccagcgat gaggaactgc gcgaagcact ccagattggt ctgatcgtgg
    10921 ggggctcgat cgtgatcccc cacctgcgca gggctgaaga atactggcaa agcctcacca
    10981 cttcttgaag ataaatacca ccgccagcac gataaagcca aagcctacaa tatagttcca
    11041 gcgcagattt tccttcaagt agagcaccga gaaagcgcaa aataccacca gtgtaatcac
    11101 ttcctgaatg gtcttgagtt ggaaggcatt gaaggtcccg tgtccaatcc ggttggcagg
    11161 tacctgaaag cagtattcaa agaaggcgat cagccagctg atcaggatca ccttgaacag
    11221 cggggaatct ttatacttga ggtgtccata ccaggcaaag gtcatgaaga tgtttgagat
    11281 gctcagcagc aggatggtct tcatttcttt cttcggctac tccgatagaa gctatccaga
    11341 tcataatcca gagcaagaag tttttccaat tgcaagcctt cgcgccagga gtagcccttg
    11401 ggttcttcaa tctggagcgc gaaatttatc aacccggctg cgcctcttac agattgtttc
    11461 aggctgtgct tgccaagcat gcctgccagc aagaaagacg caaagcaatc tccaatgccg
    11521 ggatgagtac ctttattaac cgggaagatc acttcatccc atctgtcgcc ggatttgtca
    11581 taaaccccgc aaagcctgat atcaggtaaa cgggtgggga tgctggtgat cactactttc
    11641 gagggtccca gcctggagag tctgcaggca atcgcgtccc actccacatc aggatcttcc
    11701 agcaaatcct catttagcag gattgcggct tccgtggcgt tgggggtaat gagatcggcg
    11761 tgtttgatca gttttttcat cgccttcacc atgctcagat caaagcagct gtatagcttg
    11821 ccctgatctc ccaggacggg atccaccaac actaatccct tggggtccac aagggttttg
    11881 atagcctcaa tgatgctgtc cacctgatcc ggtgagccca aaaacccagt gtaggccgcg
    11941 tcaaaacgca aacccagttc ttgccagtga gcgatgaaat ccttcattcc atcatcaaaa
    12001 ggccggaaac tgaagccctc atgatctgtg ttggctgata gcatggcagt gggcaaagct
    12061 gccaccctga tccccatcct gtacagaatg gcgatggcgg ccagcagaga agtgtggccg
    12121 aagccggaaa gatcgtgggc ggcaagcacc cgtttactga tgtagtccat tggcaggttc
    12181 ccgtaatgtt ttgagacatt aaagtgaagt cgcctgtttt tgtcaatatg cctgtctctg
    12241 cctgttcgat aaaaccctgc cacttttaaa acttgacaaa tatcccgccc cgcaatctgc
    12301 ttgccgaaga tcagttcaac tataaggacg gaccatgaac acttcatttg acgtgcacgc
    12361 catctaccag ctgatgcacg acagccacat tcagttctct tataaaggtc ccatcacaca
    12421 ggacgttttg atctcactgg gcgataccat caaggaagag ctacaccggg acgattgtga
    12481 cgccaaggtg atccgcaggg tcttttccgt gttggtggaa gccgcccaca acatcctcaa
    12541 gtattcttcc gaaaagaccg ttctggataa aagcaagacg gtgggcattg gtctgatcgg
    12601 cattggcaaa actcccgaag gatgctttgt ggtgttcagc ggtaatttcg tgagtcccac
    12661 cgaggcggaa gacatcaaga ccaggttgga ttacatcaac accctttccc ccagcgaact
    12721 caccagaaac taccagcagc agatgcggca gggcaccatc agtgccaacg gctcagctgg
    12781 actgggcctc tacgaagtgg cccgcaaatc cggtcggcct atcgattaca gcttcacccc
    12841 tggtccggac ggcatgatgt tttttgagat gaagatcgtg gtggaagtgg aggattaatg
    12901 atgaagaaac tgcatataga aggcacccgt tacagcccca gaatcgagct tgacccagag
    12961 accaggaccc ttctgatcga gggtgacagc taccctgaaa acgccgtgga cgtctatggc
    13021 cctctgatca ccgctctgga tgaatatttt gaacagccca acacccctct caacatcaaa
    13081 atgctgagct tcttccaaaa caccagttcc agcaagatga ttaccgatat gctggccaag
    13141 ttacagcgtt tcttcgatca aggccatccg gttgagctta cctggtttta cccttcggac
    13201 gatctggaga tcaaggatac cttcgagctc tttctggagg atgccagctt ccctcatagc
    13261 attcttcaac tagaggaata ggcagcaatg gggcaaaatg acctgtttgg caaagaacag
    13321 gaactgctgg atcgggcaaa agcacttctg gagagccata aaagcgaagg ctgcgttacg
    13381 atagaggatt ttcaggccct cgctaccgcc tattcagaac tcctcaaaca gagcagccag
    13441 ctcacccgcc tcagcgataa acaacagaag cgcatgaaca ctctgatgga caggctgtca
    13501 cgctatgtgc cccctcccat ctataaaaag atcacttccg gcaaggaaaa ggtggagacg
    13561 ggtacgaccc gcagagtgaa actgagcatc ttcttcagcg acatcaaagg cttttcctgg
    13621 catagcacca agatggaagg cgaagccctc accgccatcc tcaactccta tctggaagag
    13681 atgacccaca tcatcttcaa acacggcggc accctggata aatacatcgg cgacgctatt
    13741 gtggtgttct ttggcgatcc ggactttagc agcgacgcgg aacatgcccg ccgggctgtg
    13801 atgatggcac tggaaatgcg gcaacgcatg acggaactgc agcacaaatg gtttgaacta
    13861 ggctttccgg agcccctgca catccgcatg ggcatcagca ccggatttgt gaacgtgggc
    13921 aacttcggat ccagcgaaca catggactac accgtgatcg gagcacccgt gaacatggcg
    13981 gccaggctgc aagcggaagc ctctgaagac cagatcctca tctctcacga aacctggagc
    14041 tttgtgaaag acgtcctgga ctgcactgaa ggccgctact atgatctcaa gggcttctac
    14101 aatcccgtgc tggcctatga ggtgaaaggc ctcaagctcc agtccccgga acgtcaacgc
    14161 gtgattgaag acgactccag cggcatctat ctgcgctacg atccccaaaa actgagccgg
    14221 gatacccttt tggacattat ccacaaactg gagaacgacc tcagcggcga tccctccatc
    14281 gaataattac ctctctgtaa aggagttgct atgcgaccca agctaagctc catcatagta
    14341 ttgatctgta tcgtcctctt gcctctgctg acaggtgcgt caccggaagt acagaaggcc
    14401 atagaggaat atcagagagt gcagaatacc aaccgcaggg tgaggatttt cccgtttgat
    14461 gaggggaatc cgagctattt gggttattct ttcacagann nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14581 nnnnnnnnnn nnnnnnnngt ttgtcgatat gtttgaaggc tatcctgatg ttgagcgcga
    14641 ttcattgcta ctcggtagca atgcacaaac atacgatgga tcaatctatg tggccggaat
    14701 ccactactta tataagtggc aaggccatgc atggaagcgt tacgcgttta ctaagaacga
    14761 tgccatccag tttgttaaag cctgtggaaa caaggtgctg tgtattggta aaaccagtta
    14821 ctgtgtctta acaggcaatt catggacaag ctataaagct gagtcatggt tacttgatgg
    14881 tttggataag cagcgcttca tgataactgg tatggatgag tgggaacccc aatatttttc
    14941 agcagtaacg cttcttcctg aaaatcagct tgtggtttgc aatatacaag gtgagtacaa
    15001 tttaaaaacg ttggccaaac gctgtaagga tgacggtgta cctgtcttca tcaacatcgg
    15061 atctaatggc aaatctgatt ctaccatagt cttgtcatct gaattaaaga aacaacaaat
    15121 cgctgacgtc aacctgatag aaccacaatt ccatcatgga acagggagtg acatatggct
    15181 ttacctccgc cacacaccct acttctatta ctttgactca aaaaccaggt ctttcaaacc
    15241 tagttggtat cccgagggaa cggagatctc agggatcatg aggtttaagg agcccttaac
    15301 catcagttgg aaaaccaaaa ccttatctat cagggagctg ataatctaca cacagaatgg
    15361 aaaagtggta gctgaatcac ttggtccagt cgacacggaa ggcagattca ggattgaggg
    15421 aaccctgccg gatgccgtgc ccggaatgaa gtatcagttc atcatatcaa ttgatgacac
    15481 tgcttacagc agcagctacg ctcaatatcc accagataaa caagttcaga ttgcctatca
    15541 ttatgacagt aaacagaaca agctcataat gcgtgattta tcatcgctga ggagcaacgt
    15601 tcataatcag ctgcctttcc ccgatctagg cttaacaaac agcaagacat ttgtatataa
    15661 taaaaggtct atcactgaag gtaatattcc ggggatacac gcattcaatg cccttggtct
    15721 gaaagataac gaaagatatg attttagcat tactgaccca ggcaagagat cggaattcag
    15781 ctaccttgat cccgtggcca aaacagctct aatcacaact cagtattcac attacttagt
    15841 accgcttgcg aagcctgtaa agccattaaa cgccttcgat atctcacagt atagcgagct
    15901 tgaaactcat cgttggcctc agaatagctt cattagctct ggaatgtact cgcaattttt
    15961 gttgagaagc agagacattc gccctggcca ggaagcagat atccataaca ttgacggtca
    16021 gagcgtcacc cctttggtaa gagaaaagtg gctcgagtgg tctcataatg gagcaaatag
    16081 tttcattttg gtaggtaggg tggaaggaag ccgcaaacat atctttgagt ggaatttgaa
    16141 tagtaacagg aagttgccga taacttcaac cgataagaat accagaatta tccaatccac
    16201 cgatggggca ggtgttatcc tcataaacga tcaggaccta ggttttcagc tgtggcacaa
    16261 agcagaccca aactcagatt tcaaattgac atcctcgggc agttttgatg ccgcagcgaa
    16321 agttttagat accaagctaa aatcagatca aggctggggt tacaagatac tgagtttcct
    16381 gaaaaatggg caaactatcc tcttatcaga tgggaagtta gcttgcctgc ctccccttga
    16441 tttaacaatg gcaagcgata gttccatcag ctatggagaa cgttactatc ccagtctcga
    16501 tcccaaagac aggccccgag agtttacagc cagcaagaac aacaatgagg tcagcatcaa
    16561 cgttgtacct tttatcttta atcccgtttc cggagacctt gaattcaaac ctgattggat
    16621 tgagatttac cagttaggat caaagacact cgtcagctat tatgaaatgg atgacaagaa
    16681 ccagtactac taccacagca gcgtcctatc gggatggaaa gcagataaga gccccaatga
    16741 cttccgaatc ctatgtgcca acggcaaaga tccagtccgg caaagattgg cggatgggga
    16801 ttacgtacta tatgaaacca atgatggcaa gctatactac tgcacacaga atgggtggaa
    16861 ctacttagat attaaaggct acactggtga ttatggtcca ttgcttgaag tgattagtgt
    16921 aagcccaatg atgacaagaa gcagtcatca gatttctacc ctccaaccat ccacaatagc
    16981 aagatctacc ggtccagcca tgtatgctga cgacgctgtc ctggctttaa ggtttgagcg
    17041 agggattgtc aaataccctc tctccggaga agtcgtttca gtgattacca acacggacgg
    17101 catagagttt aacaaccgta ccggcatggt ctttgactgg caacatcaaa ggctgctggt
    17161 ggctgatgat tacggcattt acgaaatcag ttcaccctcc agttcagcca aactccacat
    17221 cccctggctt gaaaacaacg gcaaccgcat ccccagatca gatttcatta ggataaagta
    17281 caagcaacgg gatctgagga tacccataaa cattctccaa tctccagatc ccaggcgttt
    17341 ccagatcaag tatcagttga ttggttacga taaggaagcc cgcctgagag agtggtcacc
    17401 ttacctggag tacaacaggt tacctgctgg taaatataca ctttccgtga tcgcctattc
    17461 acctgatgga gtgcctaccg acgagctcaa agtccccatc cgcgtccttc cacctctctg
    17521 ggacacctgg tgggccaggc tcatctatgc cattgccatt ttctttagcc ttagggccat
    17581 tatgagatgg cggctcaaac gtttggagcg cagaaacaaa atgctggagc aggctgtcag
    17641 cgagcgtacc atcgaactca gcgaaaagca gcgtcgcatg acggaatcca tccaatacgc
    17701 ttcgctgatc cagagatcta tcctgcctca gacagcccag ttaaacgagt tgttcagcag
    17761 tcatttcgtg atctggaagc ccagagacat cgtgggcgga gatttttact ggctgcatca
    17821 caagccggaa agcccgcgct ggctgtttgc cgtgatcgac tgcaccggtc atggcgtgcc
    17881 gggggccttg atcaccatga cggtaaactc catcctcaat catctggtca cggaccaggg
    17941 catcgaagct ccggttaccc tattgaagga gttacattct cagctggggg ccgctctgca
    18001 tcaggaagag gaccgcaccc agcaggatgg cctggagatt tccatgatcg cggtggataa
    18061 gagcaaatcg gagctcgagt ttgcgggggc gggattgcat ctcattcatt ggcatcccga
    18121 gctggaaggt cctgaactga tcagaggtta caaacacggg ctgggaggcc tgaagcgtta
    18181 taaacagctc gatctgagca gcagcgtagt cagctatcgg caagacagca tcttctacat
    18241 gtacaccgac ggcattatcg atcagcccag gaccaacgac gagcgtcgta tccgctttgg
    18301 ccatccgcag tggctggaat tcatcaaccg ggtggcagtc tatgacctga tgaggcagca
    18361 ggaacgcttt gaaggcaagc tggaaagcct gctggagata catgaacagc gggatgacat
    18421 cacggtggtg gggatgagga tgtgagagct ggcgcagtga gat
//
LOCUS       DAOSFN010000037        18202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2494_length_18202_cov_8.075219, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000037 DAOSFN010000000
VERSION     DAOSFN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 18202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18202
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2494_length_18202_cov_8.075219"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /gene="purL"
                     /locus_tag="PLO35_03905"
     CDS             complement(<1..1950)
                     /gene="purL"
                     /locus_tag="PLO35_03905"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOH78841.1"
                     /translation="MNDQIISPELISKHGISPDEYSYILNILGREPSLVELGIFSVMW
                     SEHASYKNSVKLLKTLPKEGPHMLAKAGEENAGVVDIGDGLAICFKIESHNHPSALEP
                     YHGAATGVGGILRDIFTMGARPIAALNSLRFGAPDDPHTRHLIREVVHGIADYGNCFG
                     VPTVGGEIYFEDCYEGNPLVNAMAVGVVAHDQIARSAAKGIGNLVVYVGAKTGRDGIH
                     GATFASVDLSDQSAEMRTAVQVGDPFYEKLLLEATLEVINAGLVVGIQDMGAAGLTCS
                     SSEMAGKGEVGIELDTALVPQRETGMTPYEIMLSESQERMLIIVRPDDFNALRDVFHK
                     WDLDAVTIGRVTDDKLLRIKHNGQTVAEIPPEYLVLGGKAPVYTRETEEPEYYQRLRN
                     TDLSALEIPGDFNAELLAMMAHPNLASRRKVFRQYDHMVQIGSTVEPGSDAAVVAIKG
                     TGKAIALSTDCNSRYCYLDPYHGARAAVAEAALNVACAGARPVAITNCLNFGNPLKPT
                     VYWGFSEVIRGMGDACRALETPVTGGNVSFYNENPTGAIYPTPVIGMLGILDDASKAL
                     TQHFKAAGDLICLLGALPTGLDASQYLEHRYHLVAGKVPQVDLAFHKRLIDLLVELSQ
                     QALVRSAHDVSDGGLAVALTECLLTH"
     gene            complement(1947..3080)
                     /locus_tag="PLO35_03910"
     CDS             complement(1947..3080)
                     /locus_tag="PLO35_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOH78842.1"
                     /translation="MKSLIAPSLRKLQKHLSKDDMTGKTILHYTGSMFGIGCKVSDAE
                     TIRRIVKLKQRDDNSGFIALVPDIRWFDDAEVHIPERLKPLLEQYWPGNLTAVFRCDH
                     PMFDKLRVNGKVAFRVPNDDLLRVFIEMLDEPIVSTSVNISTLPAENDLGRLNSFYAT
                     WFDFAIYPSPRTISDEPKASTIIEYLSSNDPANPEGVARIKCLREGSIPFYGIKKSFE
                     MPTVTFVCTANICRSPIAEKLFNKLARERRINVVADSCGLIEGGHMISANSMQLLMEQ
                     GIEEAQSHMSKQITPEIVSESWLLLTMEERQRDFIREKEPNSSHKVMTLNEIVGESGD
                     IKDPYGSELDNYRKTFILIEERVNRLLDMIQNKAITLKPGSGK"
     gene            complement(3099..4199)
                     /locus_tag="PLO35_03915"
     CDS             complement(3099..4199)
                     /locus_tag="PLO35_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOH78843.1"
                     /translation="MHRSLTLLILLVLAFSAPGDELSDKQKQLQKVQNQLQNAQKKVK
                     EAESRKKKADSEITRTASLKRRVDDNLNKLKKVEEVKKDSLDSIASRLENAETNLRAM
                     DMLQNITVEMLLRADRSYTPTHIKHRDQRLLARIAEYNDLSLLSLRGFRSVLAQERQL
                     RESDLGEVVTQVRVQATEANKMDKTISALRSESTKLSQQQKNLQAQITKLKKDASQLE
                     SLISRLTGSVKTSTPSTYKFTGKKISWPVRGKIIRSFGEESRAYGTSVSNNGIDIAVA
                     EGTAVVAADDGVVVFADRYGGQGNLVIIDHKNGFFTVYAYNSSISVSMGAKVKKGQTI
                     ARSGSTGSASEPSLHFELRKDGKSINPIPYLD"
     gene            4484..6091
                     /locus_tag="PLO35_03920"
     CDS             4484..6091
                     /locus_tag="PLO35_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH78844.1"
                     /translation="MEKLMLLGDEALAQGALDAGISGFYAYPGTPSTEIVEYVQRSRQ
                     AEAAGVHRKWSSNEKTAVESAIGMSYAGKRTMACMKHVGLNVAADPFMNSAFTGANGG
                     LLITVADDPSMHSSQNEQDSRFYGKFAMIPVLEPSNQQECYDMAFWGFELSEKYHVPV
                     LIRLTTRLSHSRSGVSRRNPLPQNQMKKPDDLFQFMLLPAIARKKYKHLISLQSAFQE
                     EAMKSDFNSFNMQAPDTSLGIIATGLAFNYLMDVYQGQPVPHPVLKICQYPLPERAVR
                     ELYDKCDALLVLEEGMPLVEDMIKGLAFSGSKPIKGRLDGTIDRDGELNPNKVARALS
                     LTDTYGEDVPAVVAARPPALCVGCPHADSYLALNEAVAEYGRGHVFSDIGCYTLGFMP
                     PYNAINSCVDMGASITMAKGASDAGLFPAIAVIGDSTFAHSGMTGLMDCVYENSNVVI
                     VILDNATTGMTGGQEYTAAGKLEDICQGIGVHPDHIRVFVPFKKNFDDMVRIYKEEIA
                     YQGVSVIIPRRECVQTLKKKNKMETPA"
     gene            6088..6660
                     /locus_tag="PLO35_03925"
     CDS             6088..6660
                     /locus_tag="PLO35_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOH78845.1"
                     /translation="MKKDIILAGVGGQGILTIAAILGTAAIKRGWHLKQAEVHGMSQR
                     GGDVQSHLRLSDQPIWSDLIPFGGADMILSVEPMESLRYLPYLSKEGWIIANRTPFTN
                     ISNYPNIEDIYAAITKHPKHVLIDADAISKEIKASRAMNIVMLGAAIGHIGFSEQEIS
                     DAIRETFSRKGETIVETNLRALQAGIQAQT"
     gene            6734..9730
                     /locus_tag="PLO35_03930"
     CDS             6734..9730
                     /locus_tag="PLO35_03930"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOH78846.1"
                     /translation="MKRKSLSGSFKWQAVCLILIIFFGTGLAAQSGDYRFTVIQDFES
                     GQIELTSWADEDVSPNSWQLTSSVTHQGSAWALQLSGNTWKQQTISPVVVDSGAVFEM
                     AARTSSGANVQGIGFCDGTHALFYSISGNRVLDLEEWIPVYQGAFSNGVWNSYQFPIA
                     DDWWSFFDYLPTITSLIYVNDLDGVNNRSVYFDNIINISTDIPTAPNVSISQQVTLNS
                     FVASGTRDVGVQFQAIVSDPDSDTFTYLWEFGDGQSSNLAAPYHLYTVADDHPYRVHL
                     KVTDATGRWGLASSLVNVDPGNGSLPLKLNFVGDIMLARRYEQGGGIIPTQGVNAIFA
                     PTLDLLGNAADITSANLEVVLSNVGSPHPTKSVVYRGSPNNISGLVYAGIDKVTTANN
                     HTLDYGLEAMQQMQGLLDQNGIIHNGAGANSYQAYTPAFTNRSGLNIAWLASCDRTGQ
                     YNNAQPFLQAGYNKPGFAYMTPYYMIQQLAAVEGIADLKIMELHGGSEYSLAPGAGYD
                     KNNPFLGDDQDEDYSHRTDVPHMWDLEIRRHAIDSGADLVIVHHPHIVQAMEVYNGKL
                     IAHSLGNFVFDLDYPETMPSLILYADADHNGFSNFLVRPVYIDAYVPKPANGRLGGYI
                     LEYLAHQSALLNTKLLIDYADMSAQVVMDDSEAQASYHSYQYNHEIAPQGAWNRSLPF
                     KLPRYGSITSVDQVSPVSDAQARFGTETIWYGNFEDEGSSLWDVSSFSTTDVLDGARS
                     AMIAPNYNQTSTATIKSRCKWYDNTKSFILHGWIKTRNATNANILIRYYNTRTGGQIG
                     NESITANINGSTDWTWYYKELTVPANAWYYDIRLTCTSNTSGTVQALFDNVGLIEWTP
                     WQNASELLQIMTPNYYHWCQLQTQENPKSLNFAFTELLFSPAGSRKETSVPALLKLKS
                     WPNPFSRELNIAFDLDAKGPVIVDVYNLRGQKLRSLISGELPAGKHQAVWDGRDSHGR
                     QVASGMYFIRLKQDKASAVSKVILLR"
     gene            9836..11182
                     /gene="xseA"
                     /locus_tag="PLO35_03935"
     CDS             9836..11182
                     /gene="xseA"
                     /locus_tag="PLO35_03935"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOH78847.1"
                     /translation="MEELMIFTVYEVSAHLRQVIETQIDELYVRGEVSNYTHHSSGHI
                     YFNLKDDNATLRCTFFRNANYRLNFQLKDGLEVICHGKLTTYEKGGTYNLNVQSVSLS
                     GKGDLGLRFEQLKQKLNEEGLFDARHKQALPRYPQRIGIVTSPTGAALQDIGNILKRR
                     FPVAVEVFPALVQGPDAPAQIIAGLRYFDSRDDIDLIIVTRGGGSQEDLWCFNDEALA
                     RTIFSCRLPVISAVGHEIDFTIADFVADLRAPTPSAAAEQAVPDKKELLSYLRSVSGR
                     MRLLAESRLDKATALYSNTRHAFLRFHPEARFQSMQQRFDLLVSEFEHKTDFLTARRH
                     QVALVSRDMAMTGRNLSLRISEKLNSLQRNEQIRLRELAGQLLEKNRNILDQKAEVLK
                     LNSPVELMARGYAFVSREGDHVNSIQQVSPRDELLIYLKDGRLRSSVIECLKDSTD"
     gene            11179..12660
                     /locus_tag="PLO35_03940"
     CDS             11179..12660
                     /locus_tag="PLO35_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH78848.1"
                     /translation="MKLLKNFLLLLLFLLPLGCLAGEIRAIWVLPWSITSPQQIDKVI
                     ADALAAGQTDLFVEVRYRSDALYTPNRVPDDYPNPELRSYILADNGFDPLEYTLRKAQ
                     TQKLRVHAWVVVFNATPTHQSYLQNNFIYRNHYEWITHDSSGRKMNSGEQYGYFIDPG
                     VPAVQDHVLNVLCDLVSGYPQLDGLHLDYIRYPSSVWGHHPISVQRYNNHVIQYGPKS
                     WNQWRTLQITEFVEKTYRQVKKINPALVVSAAVFANYDDATSSYAQDWKDWLDKGIVD
                     CLYPMAYHVDLNEFGRQLDFMKSLEQDDRFVIGIRAWDANGGSLSPSNYRAYAGYTIN
                     NIFPRIDIVRSFGFAGVALFSYDGLIKENALAQLAAVSFRRDLIDTIDAARNDFPIDV
                     SPSDMSKPFAADFKVTLSGKLYELQLLIPEEGNWVWEIWDKGDRLLYKRNRYYRKGHI
                     SDMWNGALSADSKIDPGAYIVRVYPEDGKYQYFIPITIDTLKQ"
     gene            12657..14132
                     /locus_tag="PLO35_03945"
     CDS             12657..14132
                     /locus_tag="PLO35_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH78849.1"
                     /translation="MMKDRTQQAGIITIAEFTRFGIKSVIGIVLARLITPAELGSYRQ
                     LFLIYTTFSTLMMLGIPQSLLYFLPKLASDEERRQHVGKIMNLSLVLSIVFALGLLGF
                     RGLIASKFSNPQLGFLLLVYAIYPVFMFSSSLYSYVMLGQGDAVASARFTLFSVATDA
                     ILILGTALWTRDLKLIVTGVIAAAFFQWLYTRVKLQRYRASFRLDPAFYKKQFAYALP
                     LGLSSIIGMLSIQLDKFVISGFFDPASFAIFSVGAMELPFISILTNSVNSVLLPGISA
                     QSDKTKMSEVFRAAVRKNALFIFPVALICYLFAPQIITLLYTDVYRGAIPFFRIYLGI
                     LPLRVATFGIIFMAIGKTRVILFNSIFTLICNLVLNLVLVRQYGMMGAAVATVIMTAI
                     SVLLYIGLVRWKYGFIIGRLIPSLALVKTALAVLLAGLACWSILSVTNSAGMQLLAVF
                     IFGSIYLAAAYLIGALLPYDLRTALDFVKGLMAKVLGSRAK"
     gene            14129..15490
                     /locus_tag="PLO35_03950"
     CDS             14129..15490
                     /locus_tag="PLO35_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH78850.1"
                     /translation="MKRILYISFYFPPIGAIASLRAAKMVKYLGRQGYEISVLCASAT
                     LMKTPRDASALRDIPPHVKVFRSLYPDPAWLYKLLYGIRLAGVVRWFDRQIFFPGSES
                     LWLPFAMTRLDTMLKNGFQPDLAIVSFGPPAVLKLALDIKRRYRIPYILDWRDEWTNN
                     PELTNSASYARASAREQNLEQKVLQNASGSVYLTTRMRDNFVNRYAWLKDLPHALIPN
                     GFDEEDFSTLKIKNQLNPSEGKSLRIHYSGSFYDRRQPDPLWQALLELIGEQQIPSDG
                     INIQIRGKNQPAFVLGRFFADPILSEVVKFLPFQPYRYNLREMMAADALLLYIPSGRN
                     TDSVLTGKIFEYLRCSKPVLAIIPEKGAAAELLREAGIGFIASDTDHAGIKNQIMRLY
                     RSWSKGDINSLAVDPEFLATYSRSAQAERLAQLIESLELDNHQADLRPDSVNSHHHEV
                     QVE"
     gene            15483..16547
                     /locus_tag="PLO35_03955"
     CDS             15483..16547
                     /locus_tag="PLO35_03955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOH78851.1"
                     /translation="MNKTKALLVLPSQSTFSELDRELLSRLCDLDCVYLEQNSSRKTY
                     LVRMIAMLGRILCGGHSLIIVWFADYHAAMAIGAAKLLGKKSLVFIGGYDAVCYPELG
                     MGVYCNAIRSLCASFALRNCSLIVANHESLLRSNNLYYRQQGHPDGVYNLIPALKTPA
                     RVIYNALSIPAPDPEELSKPRVDRFLCVGTTPRYEDTINKGYDLIAQAAKLNPDRQFV
                     IVGIADRWQARFSRQHGLETCPNLKIHSYLPHGEVLELMRTSRCFIQASISEGMPNAL
                     MEAMAMGCIPIGSRVAAIPTLIGEVGIIVEHRDLACLNAAISESSNLTISPTLISKRI
                     VDVYSQDRRYKELKAAIMEL"
     gene            complement(16572..18107)
                     /locus_tag="PLO35_03960"
     CDS             complement(16572..18107)
                     /locus_tag="PLO35_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78852.1"
                     /translation="MKYLLHGLRFPVCRDYDLAERVSAHLGLAPSSFRILKIIRRAVD
                     TRKHNLPFYDFSVELSIDTCVPEHKDLQAIKAVQTSQAQKIVVHDAHPLIAGMGPAGL
                     FCALGLVENGLKPILIDRGDDLDQRAKKVRQYWSGDDLDPESNLQFGEGGAGAWSDGK
                     LTSRSKDPLSERVFEIMTRLGAPAEIAWEALPHLGTDGIRALVKAIRKTLIEAGCEFH
                     YRSKLEDIRLQAGKLKAVKVNGEWHPSSLLILAPGNASRDSFRMLSARGVTMEAKPFA
                     AGFRIEQSQARIDLAVYGSHKWRELLGPASYRLTDRRTGTYSFCMCPGGMVVAAASEP
                     NTIVCNGMSYADRGAELCNSAIVCGVDEQIFGTGRFAGMEWQERVEKSAWKPGNLAPA
                     IRAEDFLKGRISNGVMTGSYLPGIRPADFSAFLPSTLLTRLRGGLSSFAAILRDFNRD
                     AVLIAPETRTSSPLRFPRDPVTMSCRDIPNLFVIGEGSGYAGGIISSAIDGYRLGSRV
                     VSE"
BASE COUNT         4285 a         4921 c         4584 g         4412 t
ORIGIN      
        1 atgggtaagc aagcactctg tgagggctac cgccaatcca ccatccgata catcatgcgc
       61 actgcggacc aaagcctgct ggctgagctc taccagtaag tctatcagac gtttgtgaaa
      121 agccaggtcc acttgcggca cctttccagc caccaggtga taacgatgct caagatactg
      181 cgaggcatcc aggccagttg gcagcgcacc cagcaggcag atcaggtctc ctgctgcctt
      241 aaagtgttgg gtgagagctt tggaagcgtc gtccaggatc ccgagcatac cgatcaccgg
      301 ggtgggatag atggcgccag tggggttctc gttgtaaaat gatacgttgc cgccagttac
      361 aggagtttcc aaggcccggc aggcatctcc cattcctctg atgacctcac tgaagcccca
      421 gtaaaccgtg ggtttgagag gattgccaaa attgaggcag ttggtgatgg ccacaggtct
      481 ggcacccgcg caggctacat ttaaggcagc ttccgcgacg gccgctcggg caccgtggta
      541 gggatcgaga tagcaataac ggctgttaca gtcggtgctg agagcgatgg ctttgccggt
      601 acctttgatg gcgaccacgg ccgcgtcgga accgggctcg acagtgctgc caatctgcac
      661 catatggtca tattgtctga aaactttccg cctggaagcc agattgggat gggccatcat
      721 ggccagcaac tcggcgttga aatccccggg aatctccaaa gcgctcagat cagtgtttct
      781 gaggcgctga tagtattccg gctcttccgt ctctcttgta tagacagggg ccttgccccc
      841 caggaccaga tattccggcg gaatttccgc cactgtctgc ccattgtgct tgatcctcag
      901 tagtttgtca tcggttaccc gaccgatggt aacggcatcg aggtcccact tgtgaaacac
      961 gtctctcagg gcgttaaaat catctggacg gacgatgatc agcatgcgtt cctgagattc
     1021 ggaaagcata atctcgtagg gcgtcatacc cgtctcgcgc tggggtacaa gagcggtatc
     1081 gagttcaatg cctacttcac ccttgccggc catttcagag ctggaacaag tgagcccggc
     1141 cgcccccata tcctgaattc ctaccaccag accggcatta atgacttcca gggtggcttc
     1201 cagcaggagt ttctcataaa aaggatcgcc cacctgtact gcggtgcgca tttcagcgga
     1261 ttgatcagaa aggtccaccg acgcgaaagt agcgccatga atcccgtccc ggcccgtttt
     1321 agcgcctacg tacaccacca ggtttccgat tcctttggcg gctgatctgg caatttgatc
     1381 gtgggccacc actcccacgg ccatggcatt taccaaagga tttccttcat agcaatcctc
     1441 aaaatagatc tcaccaccca cggtcggtac tccaaagcag ttgccgtaat cagctattcc
     1501 atgcaccact tctctgatca gatgacgcgt atgaggatca tccggagctc caaaccggag
     1561 ggaattgagg gccgcgatcg gacgggcacc cattgtgaaa atgtctctga gaatgcctcc
     1621 cacaccggtc gcggcaccgt ggtaaggttc cagagcggaa ggatggttgt gactttcaat
     1681 cttgaaacag atggccaaac cgtcaccgat atccaccaca ccggcatttt cttcacctgc
     1741 tttggccagc atgtggggtc cctctttcgg caaggtcttc agcaatttga ccgagttttt
     1801 gtagctggcg tgttccgacc acatgacgct aaaaatcccc aattccacca ggctgggttc
     1861 ccggcccagg atgttcagga tatagctgta ttcgtcaggc gagatgccgt gtttacttat
     1921 cagttccgga ctgatgatct ggtcgttcat ttcccgctcc cgggttttag agtgatagct
     1981 ttgttttgga tcatatccag caggcggttt acgcgttctt caattagaat aaaggtcttg
     2041 cggtaattgt ccagctctga gccgtaagga tccttgatat cgccgctctc gccgactatt
     2101 tcgtttagtg tcataacttt gtgactggaa ttgggttcct tttcacgaat aaaatcccgc
     2161 tgccgttctt ccatggtgag caacagccat gattcgctca ctatctccgg cgtaatctgt
     2221 ttggacatat gcgattgggc ttcttcgatg ccctgctcca tcaacagttg catcgagttt
     2281 gccgatatca tgtggccgcc ttcgatcaga ccacaggaat cggcgaccac gttgatccgc
     2341 cgctcccgcg ccagcttgtt gaagagcttt tcggcaatgg gactgcggca gatattggcg
     2401 gtgcagacaa aggtaacggt gggcatctca aaagactttt tgatcccgta aaaaggtatg
     2461 gacccctcgc gaaggcattt aatgcgtgcc actccttcgg gattggcggg atcgttggaa
     2521 gaaagatatt cgatgatggt ggaagccttg ggttcgtctg aaatcgttct gggagaaggg
     2581 tatatggcaa aatcaaacca tgtggcgtaa aagctgttca acctgcccag atcgttctca
     2641 gccggcaggg tggagatatt gacgctggtg ctcacgatgg gctcatccag catctcgata
     2701 aataccctca gaaggtcatc gttgggaacc ctgaaggcca ctttaccgtt tacccggagc
     2761 ttgtcaaaca tggggtgatc gcaacggaag acagctgtca gattccctgg ccagtattgt
     2821 tccagaaggg gtttgagcct ctccggaatg tgaacttcag cgtcgtcaaa ccatcggata
     2881 tctgggacca aagcgataaa accgctgtta tcgtcccgct gtttgagctt cacaatccgc
     2941 cggatggtct ctgcgtccga aactttacaa ccaatcccaa acatcgatcc cgtgtagtgc
     3001 aggatcgtct tgccggtcat atcgtctttg gataggtgct tttgcagttt tctaagcgat
     3061 ggggctatca gactcttcac acacgctcct aattctattt aatccagata cgggatgggg
     3121 ttgatggatt tgccatcctt gcgcagttca aaatgcaggg aaggttcgct ggcggaaccg
     3181 gttgaaccgc tgcgggcaat ggtctgacct ttcttgacct tggcacccat agagacgctg
     3241 atgctgctgt tgtaggcata gacggtgaag aagccgtttt tgtggtcgat gatcaccagg
     3301 ttgccctgtc cgccataacg atctgcaaaa accacaacac cgtcatcagc cgccaccact
     3361 gcagtgcctt ccgcgacggc gatgtcaatc ccgttgttgg atacactggt accgtatgct
     3421 cgggattctt ctccaaagct tctgatgatt ttcccccgca ccggccagga gatttttttg
     3481 cccgtaaact tgtatgtact gggggtagaa gtcttgaccg agccagtaag ccggctgatg
     3541 agggattcca gttgagaggc gtcttttttc agcttggtaa tctgggcttg aagatttttc
     3601 tgctgttggc tgagtttggt gctttcgctg cgcagagcgg aaatggtttt gtccatttta
     3661 ttggcttcgg tggcctgcac ccgcacttgc gtaaccactt cccccagatc agattcccgc
     3721 agctgtcgtt cctgggccag gacgctgcga aaacccctga ggctcagtag gctcaaatcg
     3781 ttgtattccg caatcctggc caggaggcgt tgatcacggt gcttgatatg ggtgggggta
     3841 taggaacggt ctgctctcag cagcatttcc acggtgatgt tctgcaacat gtccatcgcc
     3901 cgcagattgg tttcggcgtt ttccaacctg gacgcgatac tatccagaga gtcctttttg
     3961 acctcttcca cctttttgag cttgttcagg ttgtcatcca cccgccgttt taaagaggcc
     4021 gtgcgggtga tctcggaatc cgcctttttc tttctggatt ccgcctcttt gacctttttc
     4081 tgggcgttct gcagctggtt ttggaccttt tgcagctgtt tttgtttatc tgacagttca
     4141 tccccgggcg ccgagaacgc cagtaccagt agaatgagca gagtgagcga tctatgcata
     4201 agtataaacc tgttttacct tgtgaatcca gtttggctgg cggggctatg gtgtcaatac
     4261 aattttctgg gcttgtgcgc taaacaaccg aatccacgca agagccaatt tgcaggcctg
     4321 cattcaaccc cacgtgaaaa gtgcttgact gatttgcgcg gtttgggatc gtgtgagttg
     4381 agccaagtag ctggtcccaa actccggcac ggaacttgca tttgatgtac caagtgccga
     4441 tttaacatat aattccagaa taaaatacat ataggagtaa ctgatggaaa aactgatgtt
     4501 actgggtgac gaggcattgg cgcaaggcgc gctcgatgct ggtatctcag ggttctacgc
     4561 gtatcccggc actccctcca cggagattgt cgaatacgtt caacgcagca gacaagctga
     4621 agcggctggc gttcaccgca aatggagttc caacgagaaa accgcggtgg aatcggcgat
     4681 aggcatgagc tatgccggaa aacgcaccat ggcctgcatg aaacacgtcg gcctcaatgt
     4741 agccgccgat cctttcatga attccgcctt cacgggtgcc aatggcggct tgctgattac
     4801 cgtggcggac gatccatcca tgcattcatc ccagaatgaa caggattccc gcttttatgg
     4861 caagtttgcc atgatccccg tgttggagcc ctcaaatcag caggaatgct atgatatggc
     4921 tttttggggc tttgagcttt cagagaagta tcacgttccc gtactgatca gacttactac
     4981 cagactttct cactcccgca gtggggtatc cagacgtaat cccctgcccc agaatcaaat
     5041 gaagaagcct gacgacctgt ttcagttcat gctcctgccc gccatcgcgc gcaagaaata
     5101 caagcacctg atctcgctgc agagcgcgtt ccaggaagaa gcgatgaaat cggacttcaa
     5161 cagcttcaat atgcaagccc ccgataccag cctgggaatc atcgccaccg gcctggcttt
     5221 taactatctg atggatgttt accagggaca acctgtcccc catccggtgc tcaagatctg
     5281 tcagtatccc cttcccgaaa gggcagtccg cgagctttat gacaagtgtg acgccctcct
     5341 tgtcctggaa gagggcatgc cactggtgga agacatgatc aagggtctgg ctttcagcgg
     5401 cagcaagcca atcaaaggcc gtttggacgg taccatcgac cgagacggag agctgaaccc
     5461 caacaaggtg gccagagcgc taagtcttac agacacttac ggtgaagatg tgccagcggt
     5521 tgtggcagcc cgtccacccg cactttgcgt gggatgtcct cacgcggaca gctatctggc
     5581 cctcaacgaa gcggtggctg aatatggcag aggccatgtt ttcagcgaca ttggctgcta
     5641 tactctgggc tttatgcctc cctataatgc catcaattcc tgcgtggaca tgggtgccag
     5701 catcactatg gccaaagggg cttcagacgc tggtcttttc ccagctatcg cagtgattgg
     5761 cgattccact ttcgcccatt ccggcatgac gggattaatg gactgcgtgt atgagaacag
     5821 caatgtggtg atcgtgatcc tggataatgc caccactggc atgaccggcg gccaggaata
     5881 caccgcggct ggaaagcttg aggatatttg ccaggggatc ggagtacatc ctgatcatat
     5941 cagagtattt gtgccgttta agaaaaactt cgatgacatg gtaaggattt ataaagaaga
     6001 aatcgcctac cagggcgtct cagtgatcat tcctcgccgc gaatgcgtcc agacccttaa
     6061 gaagaaaaac aaaatggaga cacccgcatg aaaaaggaca tcatcctcgc aggagtaggc
     6121 ggtcagggaa tcctcacgat cgccgccatc cttggaaccg ctgccatcaa gcgtggctgg
     6181 catctcaagc aggccgaagt gcatggcatg agtcaacggg gcggagacgt ccaatcccat
     6241 ctgcggctat ccgatcagcc catctggagc gatttgatcc cctttggtgg agcggacatg
     6301 atcctcagcg tggagccgat ggagtctctg cgctatctac cctatctttc caaagaaggc
     6361 tggattatcg caaaccgcac ccccttcacc aatatcagca actatcccaa tattgaagat
     6421 atatatgccg cgatcaccaa acaccccaag cacgttctga ttgatgcaga cgctatctcc
     6481 aaggagataa aagcaagcag agcgatgaat attgtgatgc tgggcgcggc aattggacat
     6541 atcggtttct ccgagcaaga gatatcagac gccatccgcg agacattctc ccgcaaaggg
     6601 gaaaccatcg tggaaaccaa tctcagggcc ctgcaagcag gtattcaagc ccaaacataa
     6661 caaccattac aggggggatg gtggctgagc cccgtccccc tcgataatca cagcctggct
     6721 gtggggagat accatgaaaa gaaagtcttt gtctggatcg ttcaaatggc aggctgtgtg
     6781 tttgatcctg atcatattct tcgggaccgg gctggctgcc caaagtggag actatcgctt
     6841 taccgtgatc caggattttg aaagcggcca gattgagctg acttcctggg cagacgagga
     6901 tgtttcaccc aattcctggc aactgaccag cagcgttacc caccagggaa gcgcctgggc
     6961 tctgcaattg agcggtaaca cctggaaaca gcaaacgatc agtcccgtgg tggtggattc
     7021 gggggccgtg tttgaaatgg ctgcccgcac cagttccgga gcgaacgtac agggaatcgg
     7081 cttttgcgac ggcactcacg cgctcttcta ttccatatcc ggaaacaggg tactcgacct
     7141 cgaggaatgg ataccagttt accaaggggc attttccaac ggcgtgtgga atagctacca
     7201 gtttcctatt gccgacgatt ggtggtcttt ctttgattat ctgcccacca tcacctccct
     7261 catctacgta aatgatttgg atggagtgaa caaccgcagt gtctattttg acaacatcat
     7321 caacatcagt acagacatcc ccaccgcccc caatgtgagt atatcccaac aggtaacctt
     7381 gaacagcttc gttgcttccg gcacaagaga tgtgggagtc caatttcagg caattgtcag
     7441 cgatccggac agcgatacat tcacctacct ttgggaattt ggcgacggac aaagcagcaa
     7501 tctggctgct ccctatcacc tctacacggt ggctgacgac catccctacc gggtgcatct
     7561 gaaggttacc gacgcgactg gcagatgggg actggcgagc agcctggtga atgtggaccc
     7621 cggtaacggc agcttgcctt tgaagctcaa ttttgtggga gacatcatgt tggcccgtcg
     7681 ttatgagcag ggaggcggga tcatccccac ccagggagtg aacgctatct ttgcgcccac
     7741 gctcgatttg ttgggcaatg ccgctgacat caccagcgcc aatctggaag tggtgctctc
     7801 caacgtggga tcccctcatc ccaccaaaag cgtggtttac agaggcagtc caaataatat
     7861 aagcggtctg gtctatgccg gtatcgacaa ggtgaccact gccaacaatc acactctgga
     7921 ctacggtctg gaagcgatgc aacagatgca gggcctgttg gatcagaacg gtatcatcca
     7981 caacggagcc ggcgcgaact cctatcaggc ctacactcca gcattcacaa accgctccgg
     8041 gctcaacatc gcctggctgg ccagctgcga ccgcaccggt caatacaaca acgcccagcc
     8101 cttcctgcaa gccggttata acaaacccgg tttcgcttac atgactccct attacatgat
     8161 ccagcaactg gctgccgttg aaggcatcgc agatctgaag atcatggagt tgcacggtgg
     8221 aagcgaatat tctctggccc ctggagctgg ttatgacaaa aacaatccct ttctgggaga
     8281 cgatcaggat gaggactaca gccaccgtac cgatgttcct catatgtggg atctggagat
     8341 caggcgtcac gccatcgaca gcggtgctga cctcgtgatc gtccatcatc cccatatcgt
     8401 ccaggcaatg gaggtctaca acggcaaact gatcgcccac tcgctgggaa actttgtttt
     8461 tgatctggat tatccggaaa cgatgccatc cttgatcctc tatgccgacg ccgaccacaa
     8521 cggcttttca aactttctgg tccggcctgt ttatattgat gcctacgttc ccaaaccggc
     8581 taacggcagg ttgggaggct atatcctgga ataccttgct caccagagcg ctttactgaa
     8641 caccaagctg ctaatcgatt acgcggatat gtccgcccaa gtggtgatgg acgattcaga
     8701 agctcaggcc agctatcaca gctatcaata caatcatgag atagctcccc agggagcgtg
     8761 gaatcgcagc ctgcccttca aactgccacg ctatggatcg atcacatcgg tagaccaagt
     8821 gtctcctgtt tcagacgctc aggccagatt tggcactgaa accatctggt acggcaattt
     8881 cgaagatgaa ggcagttcct tgtgggatgt gagcagcttt tccaccacgg atgttttgga
     8941 cggagcccgc agcgctatga tcgcccccaa ctacaatcag acctcgacag caacgataaa
     9001 aagccgctgc aaatggtatg acaacaccaa aagcttcatc ctgcacggat ggatcaagac
     9061 tcgtaacgcg accaacgcca atatcttaat ccgctactac aacacccgca ccggcggtca
     9121 gatcggcaac gagtccatca ctgccaacat taacggctcc accgattgga cctggtatta
     9181 caaggaactc accgttccag ccaatgcctg gtactatgat attcgtctca cctgcaccag
     9241 taacaccagc gggaccgtcc aggcgctgtt tgacaacgtg ggcctgatcg aatggacacc
     9301 atggcaaaac gcgtctgaac tgctgcagat catgaccccc aattattatc actggtgtca
     9361 gctacagacg caggaaaatc ctaaatccct aaactttgcc tttacggaat tgctcttcag
     9421 tcctgccggc agccgtaaag aaacctccgt gccggcttta ttaaagctaa aaagctggcc
     9481 taatcccttc tcacgtgaac ttaatatcgc ttttgatctt gatgccaaag gacctgttat
     9541 tgttgatgtc tataatctca ggggtcagaa gctgaggtcg ctgatcagtg gagaactgcc
     9601 agctggcaaa catcaggcgg tttgggacgg tcgtgacagt catggccgcc aagtggcttc
     9661 agggatgtat tttatcaggc ttaaacaaga taaagccagc gcggtgagca aggtcatcct
     9721 gctgagataa tcatcccaga atgtgccagc ttgccgctcc aaacagctgc caataatcat
     9781 aagcagtcag aggggtgatt gacagaatct ctggcttgag atttgtggat cggatatgga
     9841 agaattgatg attttcaccg tttatgaggt ctccgctcat ctcagacaag tgatcgagac
     9901 ccagatagac gagctctatg tgagaggaga ggtgagcaat tacactcacc actccagcgg
     9961 gcacatatat ttcaatctca aagatgacaa tgccaccctg cgttgcacct tcttccgcaa
    10021 cgccaactac aggctaaatt tccagctaaa ggacggcctg gaggtgattt gccatggcaa
    10081 gctgaccacc tatgaaaagg ggggtaccta caatctcaac gtgcaaagcg tatcgctttc
    10141 cggaaagggg gatctgggcc tgcgctttga acaattgaag cagaaactca atgaggaagg
    10201 cctctttgat gcccgtcaca aacaagctct gccacgctat ccacaaagaa taggcattgt
    10261 cacttccccc actggcgctg ccttgcaaga tatcggaaac atcctcaaac gccgtttccc
    10321 cgtggcggtg gaagtgtttc ccgctctggt gcagggacct gatgctcccg cccagataat
    10381 cgccgggctg cgctattttg actcccgcga cgatatagat ctgatcatcg tcacccgggg
    10441 agggggttct caggaagacc tttggtgttt taacgatgaa gctttggcca gaacgatttt
    10501 ctcctgccgc cttcccgtga tctctgctgt gggccacgag atcgatttca ccatagccga
    10561 tttcgtagct gatctcaggg cccccactcc ttccgcggcc gcggagcaag ctgtcccaga
    10621 taaaaaagag ctgttgtcct acctgaggtc agtgtcagga aggatgcgtt tgctggcaga
    10681 atcaaggctc gataaagcca ctgctctcta ttcaaacacc cggcacgcat ttctacgctt
    10741 tcaccctgaa gccagattcc agagcatgca acagcgcttt gacctgctgg tatctgagtt
    10801 tgaacacaag accgattttc tgaccgcgag acgtcatcag gtggctctgg tttcgagaga
    10861 tatggccatg actggcagaa atctcagcct cagaatctcc gaaaaactga atagtctgca
    10921 aagaaacgaa cagattcgcc ttcgtgagct tgctggtcag ttgctggaaa aaaacaggaa
    10981 tatcctggac caaaaagccg aggttctcaa gctgaactca cctgtggagc tgatggccag
    11041 gggttatgcc tttgtctccc gggagggaga tcacgttaac tccatccagc aggtttcgcc
    11101 gcgggacgaa ctgcttatct atttgaagga tggcaggtta cgttcatccg tgatcgagtg
    11161 cttgaaggac agcacagatt gaaactcctc aagaacttct tactgctctt gctgtttctg
    11221 ctccctttgg ggtgtttagc tggagaaatc agggctatct gggtattgcc gtggagcatt
    11281 acaagtcctc agcaaattga taaagtaatc gccgacgccc tggcagccgg acaaaccgac
    11341 ctctttgtag aggttcgcta cagatcggac gccctctaca cgcccaatcg cgtgccggac
    11401 gactacccca accccgagct ccgaagctat atccttgccg ataatggctt tgaccccttg
    11461 gaatacacgc tgcgcaaggc ccaaacccag aagctcagag tgcacgcctg ggtggtggtc
    11521 ttcaatgcca cacccactca ccaaagctat ctgcagaata actttatcta tcgcaatcac
    11581 tacgagtgga tcactcatga ttccagcggc cgcaagatga acagcggtga gcagtacggc
    11641 tacttcatcg atcctggtgt ccctgccgtg caagatcatg tgctaaatgt attatgcgac
    11701 ctcgtaagcg gttatccgca actggacggc ctccatctgg actatatccg atatcccagt
    11761 tccgtatggg gccatcatcc catctcagtg cagagataca acaatcacgt catccaatac
    11821 ggccccaaat cctggaatca atggcgcact ctgcagatca ctgaatttgt ggaaaaaacc
    11881 taccgtcagg tgaagaagat caatcccgcc ctcgtagtca gcgccgcggt gtttgcaaac
    11941 tatgatgatg ccacttcctc ctacgcgcag gattggaagg actggctcga caagggaatc
    12001 gtggactgtc tctatccaat ggcttaccac gtggacctca acgagtttgg cagacagctg
    12061 gatttcatga agtcgctgga acaggacgat cgttttgtga tcggaatccg cgcctgggat
    12121 gccaacggag ggtccctgtc tccaagcaat tacagagctt acgcgggcta cactatcaac
    12181 aacatctttc cgagaattga tatcgtgagg agttttggtt tcgcgggagt cgcgctgttc
    12241 agttatgatg gcctgatcaa agaaaacgct ctggcccagc tcgcggctgt cagcttccgg
    12301 cgagacctca tcgatacaat tgacgcggcc agaaacgatt ttcccatcga tgtatcaccc
    12361 tctgatatga gtaagccttt cgcggcagat ttcaaagtaa cactcagcgg gaagctttat
    12421 gaattgcaac tgctcatacc agaagaaggt aactgggttt gggagatatg ggacaaaggc
    12481 gatcgattgc tctataaacg taaccgctac taccgcaaag gacatatcag cgatatgtgg
    12541 aacggcgcgt tgagcgcgga tagcaaaatt gaccccggtg cttatatcgt gagggtctat
    12601 cctgaagacg gcaaatacca atactttatt cccatcacga tcgatacact caaacaatga
    12661 tgaaagaccg cactcaacag gctgggataa tcacaatcgc agagtttacc cgctttggga
    12721 tcaaatctgt catcggtatt gtcctggcca ggctgatcac tcctgccgaa ctgggttctt
    12781 accggcagct ctttttgatc tacactacct tttccactct gatgatgctg ggcatcccgc
    12841 aaagtttgct gtatttcctg ccaaaactgg cctcggatga ggagaggcgt caacatgtag
    12901 gcaagatcat gaatctcagt ctggtcctct cgattgtttt cgctttgggg ctactgggat
    12961 ttcgtggttt gatcgcctca aagttcagca acccccaatt gggctttttg ttgctggtct
    13021 atgccatcta tccggtgttt atgttctctt catctctcta cagctatgtg atgctggggc
    13081 aaggagatgc agttgcttcg gccagattta cgctcttcag tgtagccaca gacgccatcc
    13141 tgattctggg aacggcgttg tggacccgcg atcttaagct gatcgtaacc ggggtgattg
    13201 cggcagcgtt tttccagtgg ctgtacaccc gtgtcaagct gcagcgctac agagcatcct
    13261 ttcgcctgga ccctgctttc tataagaaac aattcgccta tgctttaccg ctgggcctct
    13321 cttccattat tggtatgctg agcattcaac tggataaatt cgtcatttcc gggttctttg
    13381 atcccgccag ctttgccatt ttttcggtag gagcgatgga attgccattt atctcgatcc
    13441 tgaccaattc tgtgaattcc gtgctcctcc cagggatcag cgcccagagc gataaaacca
    13501 aaatgagcga ggtttttaga gcggcggtga ggaaaaacgc tctgttcatt ttcccggtcg
    13561 ccctcatctg ctacctcttt gcccctcaga ttatcactct tctttacaca gatgtttacc
    13621 ggggagctat accatttttc aggatttatc tgggcatcct gcctttgagg gtagccacct
    13681 tcggcatcat ctttatggcc atcgggaaaa cgagagtgat tctcttcaat tccatcttca
    13741 cgcttatctg caacctggtg ctcaatctgg tcctcgtgag gcagtatggc atgatgggag
    13801 cggctgtggc cactgtgatc atgacggcaa tctcggtctt gctctatatt ggtcttgtgc
    13861 gctggaaata tgggtttatt attggccgtc tgattccctc cctggccctg gtaaaaacag
    13921 ccctggcggt gttattggca ggcctggcat gctggtcaat acttagcgtc acaaattctg
    13981 ccggaatgca gctactggca gtgtttatat ttggaagtat ctatctggca gccgcttatc
    14041 tgatcggcgc gctgcttccc tatgatctgc ggacggccct tgatttcgtc aaaggtctca
    14101 tggccaaagt attgggttcc agggccaaat gaaacggatt ctgtatatat ccttctactt
    14161 tccacctatt ggagctattg ccagcctgcg agcggccaag atggtgaagt atctcggccg
    14221 gcagggctat gaaatcagcg tcctctgtgc ctcagccaca ctgatgaaaa ctcccaggga
    14281 cgcctcggca ctgcgagata tccctcccca cgtgaaggtg ttcagatcgc tttatcctga
    14341 tccagcctgg ctgtataagc tgctctacgg tattcgcctg gcaggcgtgg tccgctggtt
    14401 tgaccggcag atcttcttcc caggttcaga atcgctgtgg ctgcctttcg ctatgaccag
    14461 actggatacc atgctaaaaa atggcttcca gccagatctg gctatcgtca gttttggtcc
    14521 gcctgccgtt ctgaagctcg ctttggatat caaacgacgt tatcggatac cctacatcct
    14581 cgattggcgg gatgaatgga ccaataatcc cgagctgacc aattccgcct catacgccag
    14641 ggcctccgct cgggagcaga atctggaaca aaaggtgctg caaaacgcct ccggatctgt
    14701 gtatctgact acccggatgc gagataactt tgtcaatcgt tatgcctggc tcaaagatct
    14761 gccccacgcg ctcatcccca acggctttga tgaggaagac ttttcaaccc taaagatcaa
    14821 aaatcagctc aacccgagtg agggtaaatc tctccgaatc cactacagtg gaagctttta
    14881 cgatcggagg cagcccgatc cgctgtggca agctttgttg gaactgattg gcgagcagca
    14941 aatcccctca gatgggatca acatccagat cagaggcaag aaccagcccg cgtttgtgct
    15001 gggacgcttc ttcgcggatc cgatcctgtc tgaagtcgtg aaattcctgc ccttccagcc
    15061 ctaccgatac aatctcagag aaatgatggc agccgacgcg ctgttgctct acatcccatc
    15121 cgggcgaaac accgattcgg tgctgaccgg aaaaatcttc gaatatctcc gctgttccaa
    15181 accagtgttg gcaataatcc ccgaaaaggg agcggctgcc gagcttctga gggaagctgg
    15241 tattggcttt attgcatccg atacagatca cgccggtatc aaaaaccaga tcatgaggct
    15301 ttatcgatca tggtcaaaag gggatataaa ctcactggca gtcgatcctg aatttttggc
    15361 gacttattcc aggagcgctc aggcggaaag actcgcccaa ctgattgaaa gccttgaact
    15421 tgacaatcat caggcagacc tcagaccaga cagcgtcaat tcccatcacc acgaggtcca
    15481 ggttgaataa aaccaaggcc ttgttagtcc tcccctccca atcaactttt tccgagttgg
    15541 acagagagct cctgtccagg ttgtgcgatt tggattgcgt gtatctggag caaaacagca
    15601 gccgcaagac ctatctggtc aggatgatag ctatgctggg cagaattttg tgtggcggac
    15661 acagcctcat catcgtatgg ttcgcggatt atcatgccgc aatggcaatc ggggcggcta
    15721 agctgctggg gaaaaaatct ctggtattta tcggtggcta cgacgccgtc tgctatccag
    15781 aactgggaat gggcgtttac tgtaatgcta tcaggtcctt atgcgcttcc tttgctctgc
    15841 gcaattgctc tctgatcgtg gccaatcacg aatctctgct cagatcaaac aacctctact
    15901 atcgccagca aggacatcca gacggagttt acaatctcat tcccgcttta aaaacaccgg
    15961 cccgggttat ctacaacgcc ctcagcatac cggctcccga tccagaagag ctctccaagc
    16021 cccgggtgga cagatttctc tgcgtgggca ctactccccg ctatgaagac actatcaaca
    16081 aaggttacga cctgatcgcc caggcagcca agcttaatcc tgacaggcag tttgtgatcg
    16141 tgggcattgc ggatcgctgg caggcaagat tttcccggca gcatggattg gaaacgtgtc
    16201 caaatctcaa aatccactcc tatcttcctc acggggaggt gcttgaactg atgaggacca
    16261 gccgctgttt tatccaggcc tccatatcgg aagggatgcc aaacgccctg atggaagcca
    16321 tggcaatggg ctgcatccca atcgggtcca gagtagcggc gatacccacc ctcataggcg
    16381 aagtggggat tattgtggaa caccgggatc tcgcatgtct caacgcagcc atctcagaat
    16441 caagtaatct aaccatttct cccacattga tatctaaacg cattgttgat gtatattctc
    16501 aggatagacg ctataaagaa ctcaaagctg ccataatgga gctttagttt actttcggtt
    16561 tgttgggatc gttattcaga cactaccctg cttccaagcc tgtatccatc tatcgcggaa
    16621 ctgattatcc cgccggcata gccgcttccc tccccgatca caaagaggtt gggaatatcc
    16681 cggcaactca tggttaccgg gtcccgcgga aaccgtaaag gggaggatgt tctggtttcc
    16741 ggagcaatca gcaccgcgtc tctattgaag tctctcagga tagcggcaaa gctgctgagt
    16801 ccccctcgca atcttgtcag caaggtggat ggaagaaatg cggaaaaatc tgccggacgg
    16861 atgcccggga gatagcttcc agtcataacg ccgttactga tcctgccctt caaaaagtct
    16921 tcggctctga ttgccggggc cagatttcct ggtttccagg cacttttttc cacccgttcc
    16981 tgccactcca taccagcgaa acgtccagtg ccgaaaattt gttcatccac cccacagaca
    17041 atcgcggagt tgcagagttc tgcacccctg tccgcgtaac tcataccatt gcatacgatt
    17101 gtgtttggtt ccgaagcggc tgccacgacc ataccaccgg gacacataca aaagctgtaa
    17161 gtgcctgttc gcctatccgt aagcctgtat gaggcagggc ccagcagctc cctccattta
    17221 tggctgccat acaccgcaag atcgatccgg gcctgagatt gttcgatcct gaagccagcc
    17281 gcgaaaggct tggcctccat cgttacaccc ctcgcggaca gcattctgaa gctgtctctg
    17341 gaagcgttgc cgggggccag aatgagcagg gaactggggt gccattctcc gtttactttg
    17401 acggctttga gcttccctgc ctgcaatctg atatcctcga gtttggaacg ataatgaaat
    17461 tcgcagcctg cctcgatcaa ggtcttcctg atcgctttca ccagagccct gatcccatcg
    17521 gtgccgaggt gtggaagtgc ttcccaggca atttctgctg gggcccccag ccttgtcatg
    17581 atctcgaaaa ccctctctga aaggggatct ttggacctgc tggtaagttt gccgtccgac
    17641 caggcccctg ccccaccttc gccaaattgc aggttgctct ctgggtccaa atcatctccg
    17701 ctccagtatt gacgcacctt tttagccctc tgatccagat catcaccccg atcgatcagg
    17761 atgggtttca agccgttttc taccagtccc agagcgcaaa acaatccagc tggccccatg
    17821 cccgctatca agggatgagc atcatggacc acgatttttt gtgcctgaga ggtctggaca
    17881 gccttgattg cctgcaagtc tttgtgttct ggtacacaag tgtcaatact taattccaca
    17941 ctgaaatcgt aaaagggcag gttgtgttta cgcgtgtcaa ccgctctgcg gatgatcttc
    18001 aggattcgaa agctggatgg ggctaaaccc aaatgagcgc tcacccgctc cgcgagatca
    18061 taatcccggc agactggaaa gcggaggcca tgcaggagat atttcatagt ttatagcaaa
    18121 aaggcgcttc gcaagaagcg ccaaatctat ggtggcgaaa cccagaatcg aactggggac
    18181 acaaggcttt tcaggcctct gc
//
LOCUS       DAOSFN010000038        18011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2528_length_18011_cov_8.780352, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000038 DAOSFN010000000
VERSION     DAOSFN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 18011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18011
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2528_length_18011_cov_8.780352"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1305)
                     /locus_tag="PLO35_03965"
     CDS             complement(<1..>1305)
                     /locus_tag="PLO35_03965"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOH78853.1"
                     /translation="GNAAGNSYTAVVTVAKPEADTNTNVKLHAVVTESHIPQVWFNQT
                     TVDNVNRLMVPNQNGTEINLATGEQTTVNLQFTLNASWNVANCEVVFFLQNMTSKEIL
                     QGVKYSLASLVGYYPVSHTTIDFPDQSVGASATIPITITNFGSTTATGTITIDNPIFS
                     ANASTFNIPAAQSATVNVVFTPTAATTYNGNMTIVGNLFNHPNITIPISGTGFLNAPP
                     IAQDVVVSGPPILHQGITGSYVFSDPDGNTEGSTILKWYRIVNNVPQQIAGANTNFYQ
                     PEDADLGLALAFEVTPYDQHGLPGTPVMSGYTLPIDVLPPPQNFAALLNPPDTVILSW
                     DRPMYFDGRAFVGYRLYRNGLTISTITNSNTLTFTDTYVPVGTHEYWICSLFNNPMEL
                     SDPSNHVTVNIGVANDDQIAPPTSVSVFPNPFSDHSAFEIKTK"
     assembly_gap    1306..1405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1798..3504)
                     /locus_tag="PLO35_03970"
     CDS             complement(1798..3504)
                     /locus_tag="PLO35_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOH78854.1"
                     /translation="MDTSDKTNNEAGAKAPVPNFIRSIIETDLETGKHNKIVTRFPPE
                     PNGYLHIGHAKSICLNFGLARDFGGVCHLRFDDTNPSKEETEYVESIMEDVRWLGFDW
                     NDKLFYASDYFDRLYEYAEILVKDGKAYVCDLSPEQIREYRGTLTVPGKNSPYRDRSI
                     EENLQLFRGMRDGLYPEGSRSLRAKIDMASPNLNMRDPVIYRIKKTDHHRTGDKWMIY
                     PMYDYTHCISDALEGITHSICTLEFEDHRPLYDWFLYQLPVPCHPRQIEFARLNLSYT
                     VMSKRRLLELVETQIVNGWDDPRMPTLAGMRRRGFPPEAIREFADRIGVAKANSIVDF
                     ELLQFCVREELNRTAVRVMAVLDPIPLTVENYPEGLVEDLEAENNPEDPNAGTRKIPF
                     SRELYVDREDVSLNPPKGWFRLAPELEVRLKHAYYITLKRVEQDSQGRITHLYCSYDP
                     DSKGGWTKDGRKVKGTIHWVSAPHAISVEARLYDQLFTIADFNDVEEGKSYQDYLSPD
                     SLTVSSACVAEPSVAKAVPGQRFQFLRLGYFAADYDHNPETPVFNRIVGLRDSWAKKQ
                     PK"
     gene            3922..4257
                     /locus_tag="PLO35_03975"
     CDS             3922..4257
                     /locus_tag="PLO35_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78855.1"
                     /translation="MIARTTLKKLSLEGVIPRDRLDRSFNRAGLCAYCSRKFHCSLSA
                     DYGLVYECDDYDASDSDVPMLTFSSIGLSENKDDEDFSGLCHDCQKRDYCQLRKISGG
                     VWHCQEFIG"
     gene            complement(4626..6248)
                     /locus_tag="PLO35_03980"
     CDS             complement(4626..6248)
                     /locus_tag="PLO35_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH78856.1"
                     /translation="MRNKILAITLLAMLWTSTVCALGIDGIWISQADGSQYSFSGSSA
                     SISLLGFDLSAAKFKKHDIKYRDIGFDQKSGLYYGQNRVNDGSGNLHSWRLVSMSLPS
                     ADQMVITDVQSAASLTLVRSYWEDNIPAYYNSLNGAWKRGDDGSEYLFVQNQATLKSV
                     GPNLAKGKFQVNQVKLRNIIPLSLNNFMGENRQHKSDGTINSWDRVRISLMGDQLTIT
                     GSQAGDVLKLTRRVEPGNIVANPVVPVTNPSVSTPAVTPSPSPASTLSPSPDLVGGLP
                     KSKTQNPHAIAVVIGNRDYDESVISKVDFAINDADAIKYYLLNVFGYKESNILYYRNA
                     TLLDLYSVFGKENNHRGKLNSWVEASSVKPDVFVYYSGHGAPDVQSGSAYLVPVDCDS
                     PDRISVNGYSINVLYDNLSKLPYNTLTVVLEACFSGNSEKGLMLKSTSPVALKANVTA
                     PKVSGATIVTSTSGEQLAHWYPEKSQSLFTYYYLLGLKGEADANKDHKISTLELKTYV
                     EQNVNSYALRNRDRNQKPLFSTSEDRILVSFE"
     gene            complement(6281..7108)
                     /locus_tag="PLO35_03985"
     CDS             complement(6281..7108)
                     /locus_tag="PLO35_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78857.1"
                     /translation="MRYWLVPLLATMLGLLPLLLGAQTPAYADQLFKQRKFPAAILEY
                     ERFLYHEARSQEDSLYAQKQIMKSFYRAGMDEMLSEYGRESGLIERDREFTTRFLALS
                     ELRQGRYEVARLIPNREDGANALLMKGITELYLNRPDEAMACFGDLPSPGSGSVIYNK
                     ELLLSYCDRLKALPGRSAFLAGTLALIPGAGYAYNGKWQTALSSLLLQGAFFASAWEL
                     RRHQLPISSAMVALMGTAYYLGSIYGSASEAMRYNQEQRKKYLDSNLASWINCLEQE"
     gene            complement(7105..7356)
                     /gene="yidD"
                     /locus_tag="PLO35_03990"
     CDS             complement(7105..7356)
                     /gene="yidD"
                     /locus_tag="PLO35_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOH78858.1"
                     /translation="MWFTLYDEWISPVDGDRCRMSPSCSHYSREAIAKHGLWEGMLMT
                     LDRLQRCGYDLESYLRSYEDGIERFLDPVRGKKEQLQHP"
     gene            complement(7523..7966)
                     /locus_tag="PLO35_03995"
     CDS             complement(7523..7966)
                     /locus_tag="PLO35_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78859.1"
                     /translation="MIDSRITKLIAIVLLGAMSTSMLFSQFDVIYDTPAPVTATPMTM
                     PQQVGSASQGLLDGERDAKGNMAYGCGGFACNVFGFLAAALSNPQPSAAVVNQLAQSK
                     GADYTNAYKAGYAKKAKNQNMLYAGIGWVVGTAVLLTYFYSDPIE"
     gene            8119..8217
                     /gene="ffs"
                     /locus_tag="PLO35_04000"
     ncRNA           8119..8217
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLO35_04000"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8302..11208
                     /locus_tag="PLO35_04005"
     CDS             8302..11208
                     /locus_tag="PLO35_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOH78860.1"
                     /translation="MRLILTFIIALIIATPLIAFPVRISSWEADRDVKTLNAMHISID
                     SVNRSTGTIMAFVRHETEFQSLLAAGFDAIRLPNPAPELARKLNDPLRDGTPPQNEYY
                     SITQYLDFMVQTAAQYPNICILVTAGNSIQNRPLYFMKISDNASQDEAEPEFRYISSI
                     HGDEVVGYDMCIRLIQQLTTNYGTDTRVTQMVDNTEIWICPMMNPDGFVLGQRYNAAG
                     IDLNRNFPMPTGEQHPDGNATGQENTAIMNFSNSRGFQLSANFHGGAEVINYPWDYTY
                     TLAPDDALIQAAALTYSSHNSLLYNSTEFPQGITNGAQWYVITGSLQDWSYGYTDCID
                     LTAEIGNNKWPPASQLPTFWGYNQESMLALIEFAQKGARGTVTSVAGLPLLDATITVA
                     GNAKVTGTNPGLADWYRLLLPGSYSITAAAPGYIPQTVQLSVPTTGFVTHNFVLQLAA
                     NTYFSGQIRNADGSGISNLVVALNTVPERVVNADANGTFSFGSVPEGQYIISFRQAGQ
                     TFYSKSFLLTAENNRLVFIYSPPTTVFTDVFDNSNNWTATSPWGVTPYTGNNVLTDSP
                     GGNYANNINKSCRLTNPLNLSQIASPTLSYRTRYSLENGYDFVYVEASTNGTDWQQLT
                     SYTGSQTSWTDVSLSLAPYSGQNMHLRFRIQTDTSVNLDGIFIDDLVVKGISMANSYF
                     GDPDGDQIISSADMLIVLDYSVGLDPIPQIDPIPWNQILLFACDVDQNDVVNAVDAYL
                     IYKYITDPSYRFQAQNSLNYDFSGVTMDVDATPGFYQFSFSPSADLEVLETRLSSPDS
                     ISGWEVQFFPMGTGRCYAMNDQTKHFAWIKAQADLQYMNQSFQTPLGQIICDYALNGT
                     AGQVTLLTGSPNDDTELPAPIFSLHQNYPNPFNPNTTISFSVTDPASPSSLRIYNLKG
                     QLTKTLCHSRLETGIHKLNWDGKDDQGRSVSSGVYLYRLENGSHTQTRKMILAQ"
     gene            11205..11711
                     /locus_tag="PLO35_04010"
     CDS             11205..11711
                     /locus_tag="PLO35_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOH78861.1"
                     /translation="MKTRILSMILFLLLALTLVACFSCRPQAQEISETTAPKHVFRKD
                     GELRIKGPDGSVKGSFDIEIAETEEALQTGLKFRESMEPNQGMLFVFDGRQSYGFWMQ
                     DTYISLDMLFINYENKIFYIAENTTPFSEEPIEPDGFNLYTLELNAGTCQKLNIQKGD
                     TIEWKRTP"
     gene            11693..12655
                     /locus_tag="PLO35_04015"
     CDS             11693..12655
                     /locus_tag="PLO35_04015"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOH78862.1"
                     /translation="MEKNSLIPVLRVSALILIILLAISCGKKQGGSDASNNLQSESAE
                     YSDAVNGSDADSLKPQYYEGGASSSASAATADSTILKGFKYSWTDSKEIPPIIIIVDD
                     FGYADKDLLQGYADLPREVAFAVLPDLPATKKAAQIAQRTGHDVLIHAPMEAKQSKLK
                     PGERYIKTGQDAGTIASLIDAFHEQMPNAIALNNHMGSTATSDKTVMKAVLEHLARRG
                     LFFVDSVTSGETVGYNMANTLGQRAVRRNLFLDVPDNSDATLAAKISDLGRYKGRVEP
                     VVVITHCHNQAKLDALNKFIAQIKAMGLRLTSLSAYYGGGVALN"
     gene            12999..13676
                     /gene="cmk"
                     /locus_tag="PLO35_04020"
     CDS             12999..13676
                     /gene="cmk"
                     /locus_tag="PLO35_04020"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOH78863.1"
                     /translation="MKKVIIAIDGPAASGKSTTARLLAQRLGYVYLDTGAMYRACALR
                     AYRLGIAVDDVERVAAMVDTIVLRIDFTAGVNRVFLDGEDVSEQIRTPEISELASAIS
                     ALPPVRQKMVELQRRIAQGGGFILDGRDIGSYVFPNAEIKFFLTADPDERARRRQLEL
                     AAKGVKASLEEVRADLLQRDMNDRSRALAPLVQASDAILIDTTGLSIDAQVEKLRQIA
                     LQRLETL"
     gene            13673..14560
                     /gene="ispH"
                     /locus_tag="PLO35_04025"
     CDS             13673..14560
                     /gene="ispH"
                     /locus_tag="PLO35_04025"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOH78864.1"
                     /translation="MNIWLAEHSGFCFGVRRAIQMAREAASVGKPVCTMGELIHNPII
                     VDQLKSEGIDVCEDPFSLRDHTVILRSHGVTKDMMETLARNGNRLVDATCPYVRRAQQ
                     LVESMSEAGYQVIIMGNADHPEVIGMLSWGNASTQVLKADDRPELTGGQQLCLISQTT
                     EKPEHLADMVAWLAPRANELRVFNTICLATQQRQEASERLARKLDLVIVIGGLKSSNT
                     RQLHQLCSQYSRCFQIESEADLQAEWFQEFNSIGLAAGASTPAETIVAVYNQIKEING
                     EPDRVTAVGEIPLFKEESC"
     gene            14554..>16714
                     /locus_tag="PLO35_04030"
     CDS             14554..>16714
                     /locus_tag="PLO35_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOH78865.1"
                     /translation="MLTHDQNEVKTDQTEIDLMAGADQTSQTKEEERIPSQEATAEVV
                     NPELEPQEAEASATEEAPAPEPIEEPVVPAAEPEVEPVAEEAVPEPEAAEEPAPVEEI
                     VPEAPKPAPKPKAELSQEEKEHEELLKMYDQYTDSFESGQIKEGTVVGVTDKDVVVAI
                     GYKSDGVIPISEFAYSGIPELNSTIMVFINKVENGEGKLELSKKKADFIHNIEKIKHS
                     FTDGTILQGVVRRRVKGGMIVDVMGIEAFLPGSQMSLKPIPNLDQFIGKELSFKVVSL
                     DEDHRNIILSRKQVLEAEAAIKRADLLEKIQIGSELDGEVKNITQYGAFIDLGGIDGL
                     LHLTDMSWGKVNHPSEMLSIGDKVKVKVINFDTETQKISLGLKQLVPHPWETIELKFP
                     EGTRITGKVVSVKSFGAFVEIEPGVEGLVHISEMSWTKKIADARKILKIGDSINAIVL
                     ELDKENKRISLGMKQMDPNPWLTIEDRYPIGTVLTRKVKSLTAFGAFVEIEEGIDGLI
                     HISDISWTKRVYHPREVYRKGQEVEAVILSIDRALHRIALGVKQLSPDPWENLNIILP
                     VNTEVKGKVSKLIPKGVLVDIQLDNDVVEGFIPISHLAIPKLEHSEEAFFVGEVLPLK
                     VIELDMDNRRLILSVKAFFFSRDPKLQEEYIAIHEQYIRDRIAKNQKKKAEKQSREKQ
                     NSDKAKTDQNDEGGVAEEVMIEAETQADHVETAPALEE"
     assembly_gap    16715..16814
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16815..17132
                     /locus_tag="PLO35_04035"
     CDS             <16815..17132
                     /locus_tag="PLO35_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOH78866.1"
                     /translation="IEAETQADHVETAPALEERVTEAYSEEPIAEEVVETLAEEPVVE
                     EVVEAPIEAPVVEEVVEAHIEAPVVEEVVETLAEEPVVEEGVETIVTETEATLEDTET
                     KPV"
     gene            17140..>18011
                     /locus_tag="PLO35_04040"
     CDS             17140..>18011
                     /locus_tag="PLO35_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH78867.1"
                     /translation="MSEDIKNAMTAPQGRHPLFWLDLLLPVLLLALLTPLFHIFDWDM
                     ELQRWWFHSDTGWETGELGIFQLLYRFGNLPALILSLGAVFVLIRGFSSTRLSSWRKM
                     AIYLIAVMIIGPGLLVNTLLKDRWGRPRPRDVVEFGGKYAYEAPLQIDPTSPGKSFPC
                     GHATMGFFFFAPAFLLRKWGRKSSRSSLASSSYSLALAWFAGLFGILIGIARMAQGGH
                     FASDVVWAGTLVWIVAAGLYYLMRLDVAPFSRPRSKGNSTLKTGWKLLFWLLGLGLIA
                     GVGVATPYQSRRGY"
BASE COUNT         4222 a         4761 c         4790 g         4038 t
ORIGIN      
        1 tttggttttg atctcaaacg cggagtgatc gctgaagggg ttggggaaaa cgctcacgga
       61 agtgggcggc gcgatctggt catcattggc tacgcctatg ttgacagtca cgtggttgga
      121 tgggtctgag agctccatgg ggttgttgaa gaggctgcag atccagtatt cgtgagtacc
      181 gacaggcacg taggtgtccg tgaaagtaag cgtgtttgag ttggtaatgg tggagatggt
      241 caaaccatta cgatacaagc gatagcccac aaaagcgcgg ccgtcgaaat acatgggacg
      301 gtcccaggaa aggatcaccg tatcgggagg attgagcagc gccgcaaagt tttgaggagg
      361 cggcaacaca tcaatgggaa gggtatagcc actcatgaca ggggtgccag gtaaaccatg
      421 ctgatcgtat ggggtcactt caaaggcgag agcaaggccc aagtccgcat cttctggctg
      481 atagaagttg gtgttggcgc cagcaatctg ctgggggacg ttgttgacga ttctgtacca
      541 cttcaggatt gtgctgcctt cagtgttgcc gtcgggatcg ctgaagacgt agctgccagt
      601 gatgccctga tgcagaatcg gggggcctga aaccaccaca tcctgagcga tgggaggggc
      661 gtttaagaat cccgtgccag agatgggaat agtgatgttg ggatggttaa aaagattgcc
      721 cacgatcgtc atattgccat tgtaggttgt tgcggcggtg ggagtgaaga ccacgttcac
      781 ggtggcggat tgagcggcgg gaatgttgaa ggtggaggca ttggccgaga aaatggggtt
      841 gtcgatcgtg atcgtgccag tggcggtagt gctgccaaag ttggtgatag tgatcgggat
      901 tgtggccgac gccccgaccg actgatcagg gaagtcgatg gtggtatggg agacgggata
      961 atatcccacc aaagaggcca gagaatactt cacgccctgg aggatctctt ttgaggtcat
     1021 gttttgcagg aagaatacca cttcgcagtt ggctacattc cagctggcat tcaaagtaaa
     1081 ctggagattg acggtggtct gttccccggt agcaaggttt atttcggtgc cgttctgatt
     1141 ggggaccatc agcctgttga cattgtctac ggtggtctga ttaaaccaca cctgcgggat
     1201 gtgtgattct gtgaccacgg catgcagctt gacgttggtg tttgtgtccg cttccggttt
     1261 ggccacggtt acaacagcgg tatagctatt gccggcagcg ttgccnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnngccac cgcgtgacca ttctggagca ggtcatggca
     1441 tcccatggcc gcgcccggac agtaaccgca ccaggtgccg gtgccaactt ccacaaccac
     1501 caggttgcgg ggaacagcgc tcactgtggc caccaggcca agcagaagca gcaaagatac
     1561 taagtgtttc atattagtct ctcccatcta ggttgatatt cttcactatt aagccatagc
     1621 atttttcgtt ccgctatgat gaaatattac ggttattgac gtttgtcagc tctttatttt
     1681 tactgaaacg gatcgcaagg gctggcacca ttctttgggg gacgttttcc cggtgaaggc
     1741 cttgacagct gatcatcggg gtttgcattc tgtgcactct aatcatcaag atggcttcta
     1801 tttgggctgt ttcttggccc agctgtccct gaggcccacg atgcggttaa acaccggtgt
     1861 ttcagggttg tggtcataat cagcggcaaa gtatcccagc cgcagaaact ggaatctctg
     1921 tcccgggacc gcctttgcca cagagggctc tgccacgcag gcggatgaga cggtgaggga
     1981 atcggggctc agatagtcct gatagctctt gccctcttcc acgtcgttaa aatcggctat
     2041 cgtgaagagc tggtcataga gcctggcttc cacgctgatg gcgtggggag cggagaccca
     2101 gtggatggtg cccttcacct tgcggccgtc cttggtccag ccgcctttgg aatcgggatc
     2161 gtagctgcag tagaggtgag tgatcctgcc ctggctatcc tgttccaccc gcttgagggt
     2221 aatatagtaa gcgtgtttga ggcgcacttc cagctcgggg gccagtctga accagccctt
     2281 gggagggttc agggacacgt cttcgcgatc cacatacaac tctctggaga agggtatttt
     2341 gcgggtcccg gcattgggat cttcggggtt gttttccgct tccaaatcct caaccaggcc
     2401 ttcaggataa ttttccacgg tgagggggat ggggtccagc actgccatta ctctcacggc
     2461 ggtcctgtta agttcttccc tcacgcagaa ctgcagcagc tcaaaatcga cgatggaatt
     2521 ggctttggcc actccgatgc ggtcggcaaa ttcgcggatc gcttcgggag ggaaccctct
     2581 gcgcctcatc cctgcaagag tgggcatgcg gggatcatcc catccgttca caatctgggt
     2641 ttccaccagt tccaacaggc gccgcttgct catcacagtg taactgagat tcagccgggc
     2701 aaattctatc tggcgcggat ggcaggggac gggaagctgg taaagaaacc agtcatagag
     2761 agggcgatgg tcttcaaact cgagtgtgca gatggagtgg gtgatccctt ccagggcgtc
     2821 tgaaatgcag tgggtgtagt cgtacatggg atagatcatc cacttgtcgc cggtgcggtg
     2881 atgatcggtc tttttgatgc ggtagatcac gggatcgcgc atgttgaggt tgggggaggc
     2941 catgtctatt ttcgcgcgca gcgatctgga tccttccggg tagaggccat ctctcatgcc
     3001 acgaaatagt tgcagatttt cctcaatgct ccggtcgcga taggggctgt tcttcccagg
     3061 gaccgtgagg gtgccgcgat actctctgat ctgttcgggc gagagatcac agacgtaagc
     3121 cttgccgtct tttaccagga tttcggcgta ttcatagagc cggtcaaagt agtccgaagc
     3181 gtaaaagagc ttgtcattcc aatcgaatcc cagccagcgg acatcttcca taatagattc
     3241 cacatactcg gtctcttctt tgctggggtt ggtgtcgtca aatctgaggt gacagacgcc
     3301 cccgaaatct ctcgcgaggc caaaattgag gcagatagac ttggcgtggc cgatatgcag
     3361 atagccgttt ggttcaggcg gaaagcgggt cacgatcttg ttatgcttgc cagtctccag
     3421 gtccgtctca atgatggagc ggataaaatt gggcacagga gctttcgcgc ctgcttcatt
     3481 attggttttg tcgcttgtat ccatatgtta tacctttaaa atttacgtcg atggtcagct
     3541 ttcaggcgtg gcagttctcg tcaagtgcaa tatgcggctt tgaagccgtg agcttgaatt
     3601 gaggggactg attatgcctg tacgtgggga tttataagcc ttcaggcccc caagcgcgag
     3661 caagctttgc tccggcttag cggttccggt ggtggtggct gaagacctct ttggatgatt
     3721 gataagtgtg tcataatgta acacatggaa ggaatgacac gatttttgcc tgattgaggg
     3781 aggaaccaga gctgcgatat cagagacata agtaatagat aataaatcag atgcatcgtt
     3841 aagacagaga gtgggaccaa ttggccactt acttgcatac agtaatcatg tcagcatcaa
     3901 aaacgttggc gaggagtaac aatgatcgca agaacgaccc tgaagaagtt gagcctcgag
     3961 ggagtgatcc ccagagaccg cttggaccgg tctttcaacc gtgctggtct ctgcgcttat
     4021 tgttccagaa agtttcactg ctctctcagt gccgattacg ggctggttta cgaatgtgac
     4081 gattatgacg ccagcgacag cgatgtcccg atgctcacat tcagcagcat cggcttgagc
     4141 gagaacaaag acgacgagga ctttagcggt ctttgtcacg attgccaaaa gcgggactac
     4201 tgccagctgc ggaaaatcag cgggggagtc tggcactgtc aggaattcat cggataatca
     4261 atcatagaca catacctcaa cacacacact gggggctccc cgcccccttt ttcttttccc
     4321 cccacttttt cgtgaaaaat atgtacgcgt tattcgtttc aaacttcgga acagctcaca
     4381 tgctgccgat tgtccttact cactattgaa gtgattagcc tccatgcaag cattgtcttt
     4441 ccacgcttgc gcccttactc atggccctat tccctgagtg tatcatgtcg gactcatccc
     4501 gaaccagatt gggggtgggt acgatgagct cccggcaggc taaagtggaa ggaataaggg
     4561 cgggcaagca atgatatccc cccgctaacc atcatgccga gggctttagg cgggcagacg
     4621 tttctctatt cgaagctcac caggatcctg tcttccgagg tggagaacag aggtttctgg
     4681 ttgcggtccc tgtttctgag ggcgtagctg ttgacgtttt gctccacgta agtcttgagc
     4741 tcgagggtgc tgatcttgtg atctttgttg gcatcggctt cgcctttcaa acccagcaag
     4801 taataatagg tgaacaggga ttgggatttc tcgggatacc agtgggccag ttgctctccg
     4861 ctggtcgagg tcacgattgt ggcgccggac accttggggg cggtgacatt ggctttcaga
     4921 gcgacgggcg aagtgctttt cagcatcaac cctttttcgg aattgccgct gaagcaggct
     4981 tccaacacca ccgtgagggt attgtatggg agcttgctga gattgtcata cagcacgttg
     5041 atcgagtatc cgttcacgct gatgcggtca gggctgtcgc aatccaccgg caccagataa
     5101 gcgctgccgc tttgcacgtc cggagctccg tgaccgctgt agtacacaaa cacgtcgggt
     5161 ttcacgctgc tggcttccac ccagctgttc agctttccgc ggtggttgtt ttccttgcca
     5221 aatacggaat agaggtccag cagggtggcg ttgcggtagt agaggatgtt gctctcttta
     5281 taaccgaaga cgttgagcag gtaatacttg atggcatcgg cgtcgttgat ggcaaaatcc
     5341 actttgctga tcacgctttc atcataatcc cggttgccga ttacgacggc aatggcgtgg
     5401 ggattttggg tcttgctctt tggcaggcct cccaccaggt ccgggctggg tgacagtgtc
     5461 gaagcagggg atggggaagg ggtgaccgct ggggtgctga cgctggggtt ggtgaccggc
     5521 accactggat tggcgacgat gttgccgggt tcaaccctgc gcgtgagctt gagtacgtcg
     5581 cctgcttgcg atcccgtgat ggtgagctga tcgcccatca ggctgatcct cactctgtcc
     5641 cagctgttga tggtcccgtc agatttatgc tggcggtttt ctcccataaa gttgttcagc
     5701 gataacggga tgatgtttct gagcttgacc tgattaacct gaaacttacc cttggccagg
     5761 ttgggtccca cggatttcaa agtggcctga ttctgaacga agaggtattc cgagccgtcg
     5821 tcgcctcgtt tccaggcgcc gttgagggaa ttgtagtagg ctggaatgtt gtcttcccaa
     5881 taggaacgga ccaacgtgag ggaggcggcg ctctgtacgt ctgtaatcac catctggtcc
     5941 gcgctgggaa gtgacatcga gaccaagcgc caggagtgga gattgccgct accgtcattg
     6001 acgcggtttt gaccgtaata aagtccggat ttttgatcaa agcctatgtc ccggtatttg
     6061 atgtcgtgtt ttttaaactt agcggcactc agatcaaagc ccaggagact gatgctggcg
     6121 ctgctgccgc tgaagctgta ttggctgccg tcggcctggc tgatccagat tccgtcgata
     6181 ccaagcgcgc atacggtgct ggtccacagc atagccagca gcgtgattgc caaaatcttg
     6241 ttacgcatgc tatacctcct cggataagga cttacatgtt tcactcttgc tccagacagt
     6301 ttatccagga tgccagattt gagtcaaggt atttctttct ttgttcctga ttatatctca
     6361 tggcttcact ggccgatccg tatatgcttc ccagataata agctgtgccc atcagcgcta
     6421 ccatcgcgga agaaatcggc agttgatgac gcctcagttc ccaggcgctg gcaaaaaagg
     6481 ccccctgcag gagcagcgag gatagagcag tctgccactt gccgttatag gcatatccgg
     6541 cccccggaat caaggccagg gtgccagcca gaaatgccga tcttccaggc agggctttga
     6601 ggcggtcgca gtagctcagc agaagttcct tgttataaat aacactacca gatcccggtg
     6661 aaggcaaatc cccgaaacag gccatggctt cgtcaggacg gttgagatac agctccgtta
     6721 tgcctttcat gagcagtgcg ttagcgccat cttcccggtt tgggatgagg cgcgccacct
     6781 cataacggcc ctggcgcaac tctgacagag ccaaaaatct ggttgtgaat tccctgtccc
     6841 gttcgatcag tcctgattcc cggccatatt cagacagcat ctcatccatc ccggccctat
     6901 aaaaggactt cattatttgc ttttgcgcgt agagagagtc ttcctggctt ctggcctcat
     6961 ggtagagaaa tctctcatat tcgagaatcg cggcgggaaa cttgcgttgt ttaaagagct
     7021 gatctgcata agcaggcgtc tgggctccca acagcaaggg cagcagcccc aacatcgttg
     7081 ccagcagtgg aacaagccaa tatctcatgg gtgctgcagt tgctcctttt tgccccggac
     7141 cggatcgaga aagcgctcga tgccgtcttc ataggacctg aggtagctct ccagatcata
     7201 accgcagcgc tgcagccggt ccagagtcat cagcattcct tcccacaggc cgtgttttgc
     7261 gatcgcctcg cggctataat gagaacagga agggctcatt ctgcatctgt ctccatcgac
     7321 aggggaaatc cactcgtcat atagagtaaa ccacaggctg gcagaggatg tgggtttccg
     7381 cgtttcaggc tgagggggat aggtctttgc gagatcattc atacttacag cccggacggc
     7441 actgaagccg caaaaaacta caataagcag gaaaagtctg cttttttgta agctgtcagc
     7501 aggcatcaat ctgagcgaca acctactcga tagggtctga atagaaatat gtcagtaaaa
     7561 ccgctgtgcc cactacccag ccgatcccgg catagagcat gttttgattt ttagctttct
     7621 ttgcatagcc tgctttgtaa gcgttggtgt aatccgcgcc tttgctctgg gccaattggt
     7681 tcaccaccgc agcgctgggt tgggggttgg aaagtgccgc ggccagaaat ccaaatacgt
     7741 tgcaggcgaa gccaccgcag ccgtaagcca tgttgccttt ggcgtctctt tctccatcga
     7801 gcagcccctg agacgcggat cccacctgct ggggcatagt catgggagtc gcggttacgg
     7861 gtgcgggagt gtcgtagata acatcaaatt ggctgaacag catggaagtg gacatcgcgc
     7921 ccagcagcac gatcgcgatc agctttgtga tcctggaatc gatcattgat cctcctgaat
     7981 atatgcaaaa gatgattcat ccgaaactgg ggattggaaa ctccagctcc tccaggagca
     8041 aatctggcca tcaagcaatt tgtcaagcag aaagtgcttt tcaattgaca aaagcggcca
     8101 ctctcagaaa gtgacatctg ccaggcgcag cgcaatggat gcccgtgaac catgtcaggt
     8161 caggaatgaa gcagcattaa gcgacgtatt tcatgtgccg ttgttagcct ggcatttgtt
     8221 ttttatcaaa cggcatgaaa tatgcattca ggatgccagg cctttttata ggggccgcac
     8281 tcagtttatc agaggaatgg aatgagactt atacttacct ttatcatagc gctgatcatc
     8341 gccaccccgt tgatcgcctt ccctgtgagg atatcgagct gggaggccga tcgcgatgtc
     8401 aaaaccctca acgccatgca catcagcatt gacagcgtga accgctctac cggcacgatc
     8461 atggccttcg tgcgccatga aaccgagttt caaagtctgc tggccgccgg ctttgacgct
     8521 atccggctgc ccaatcccgc tccggaactg gctcgcaagc tcaacgaccc cctccgggac
     8581 ggcactcctc cccaaaacga gtattacagc atcacccaat acctcgattt tatggtccag
     8641 actgccgccc agtatcccaa tatctgcatc ctggtgacgg ctgggaactc gattcaaaac
     8701 cgccccctct atttcatgaa gatctcggac aacgcctcac aggacgaagc tgagcccgaa
     8761 ttcagatata tctcctccat ccatggcgat gaagtggtgg gctatgacat gtgcattcgc
     8821 cttatccagc agctgacaac taactacggc accgatacca gggtgaccca aatggtggac
     8881 aacaccgaaa tctggatttg tccgatgatg aatcccgacg gctttgtcct tggccaacgc
     8941 tacaacgcgg ctggcatcga tctgaaccgc aactttccca tgccaacggg tgaacagcat
     9001 ccggacggca acgcaaccgg acaggaaaat actgccatca tgaatttcag caactcccgt
     9061 ggatttcaac tctctgcaaa cttccacggc ggcgcggagg tgatcaacta cccctgggat
     9121 tacacctaca ccctcgctcc ggacgatgcc ctcatccagg ctgccgccct cacctattca
     9181 tctcacaaca gcctgctcta taacagcact gaatttcccc aggggatcac caacggagcg
     9241 cagtggtatg taatcaccgg ttcgctccag gattggtcat acggctatac cgactgcatc
     9301 gacctcactg ccgagatcgg caacaataaa tggcctcccg ccagtcagct gcccactttc
     9361 tggggataca accaggaatc gatgctggcg ctgatcgaat tcgcgcaaaa gggtgcgcgg
     9421 gggaccgtga cttccgtagc tggtctgccc ttgctcgatg ccaccatcac cgtggctgga
     9481 aacgccaaag tgacaggcac aaatcctggt ctggcagatt ggtaccgtct gcttttgcca
     9541 ggctcatatt ccattaccgc tgccgcgcct ggctacattc ctcaaaccgt gcagctctca
     9601 gttccgacca ccggatttgt cacccataac ttcgttttgc agctagctgc caacacgtat
     9661 ttcagtggcc agatcagaaa tgccgacgga agcggtatat caaatcttgt ggttgctttg
     9721 aacaccgtgc cggaaagagt cgtaaacgcc gacgcaaacg gaactttcag cttcggctcc
     9781 gttccggaag gccaatatat catcagtttc agacaggccg ggcagacttt ttacagcaaa
     9841 tccttcctgc tcacggctga aaacaacagg ctggtgttta tttattcacc gcctaccacg
     9901 gtgtttacag acgtgtttga caatagcaac aactggacgg ccaccagtcc ctggggagta
     9961 actccctata ctggcaacaa cgtccttacc gattctcccg gaggcaatta cgccaacaat
    10021 atcaacaagt cctgccggct caccaatccc ctcaacctca gccagattgc cagtcccact
    10081 ctgagttaca ggaccagata ttcccttgag aacggctatg actttgtgta tgtggaggcc
    10141 tccaccaacg gcacagactg gcaacaactc accagttaca ctggttctca aacctcctgg
    10201 acggacgtga gcttgtctct ggccccctac agcgggcaga acatgcatct gcgtttccgc
    10261 atccagacgg acaccagcgt caatctggac ggcatcttca tcgacgatct ggtggtgaaa
    10321 ggtatcagta tggccaacag ctactttggc gatccggacg gagatcagat catcagcagc
    10381 gcggatatgc tgatcgtgct ggattacagc gtgggacttg atcccatccc gcagatcgac
    10441 ccaatcccat ggaatcagat cctccttttt gcctgcgatg tggaccaaaa cgatgtggtg
    10501 aatgccgtgg acgcctatct gatctataag tacatcaccg atcccagcta tcggttccag
    10561 gcccagaact ctctcaatta tgatttttca ggcgtgacaa tggatgttga tgctacgccc
    10621 gggttttatc agtttagttt ttcacccagc gcggacctgg aagtcttgga aacccggctc
    10681 agttcacccg acagcattag cggatgggaa gtgcagtttt ttccaatggg gactggtaga
    10741 tgttacgcta tgaacgacca gaccaaacac tttgcctgga tcaaagcaca ggccgatctg
    10801 cagtatatga atcaatcatt ccagaccccc ttggggcaaa tcatctgcga ttatgcgcta
    10861 aacggaacgg cgggccaggt cactctgctc accggcagtc ccaatgacga caccgagctt
    10921 cccgccccga ttttctctct gcatcagaac taccccaatc ctttcaaccc aaacaccacc
    10981 atctctttca gtgtaactga tccagccagc cccagcagcc tgaggatcta caacctcaaa
    11041 ggccagctta ccaagacact ctgtcacagc cgtctggaaa ccggaatcca taagctcaac
    11101 tgggacggca aagatgacca gggccgctct gtgagcagcg gtgtctatct ttaccgtctg
    11161 gagaacggca gtcacactca aacccgcaag atgatcctgg cccaatgaaa acgcgcatcc
    11221 tatcgatgat cctgttcttg ttgttggccc tgacgcttgt ggcttgcttt tcctgccgcc
    11281 cgcaagctca agaaatctct gaaactacag ctccgaaaca cgtgttccgc aaggacggag
    11341 agctcagaat caaaggcccc gacggctcag tcaagggcag cttcgatatc gagatcgccg
    11401 aaacggaaga agctctgcaa accggcctca agtttcgcga aagcatggag cccaaccagg
    11461 ggatgctctt tgtcttcgac ggccgccaga gctacggctt ctggatgcag gacacctata
    11521 tatctttaga catgctcttt atcaattacg aaaacaagat cttttacatc gctgaaaaca
    11581 ctaccccctt cagcgaggaa cccatcgagc cggacggctt caacctctat accctggagc
    11641 tcaacgccgg tacctgccaa aaactcaata tccaaaaagg cgacaccata gaatggaaaa
    11701 gaactccctg atccccgtcc tgcgcgtcag cgctctgatc ctgattatcc tgctggctat
    11761 atcctgtgga aaaaagcaag gcggctccga tgcctcaaac aacctccaat ccgaaagcgc
    11821 ggaatacagc gacgccgtta acggaagcga cgcggacagc ctcaagcccc aatactatga
    11881 aggcggcgcc agttcctctg ccagcgccgc gacagctgat tccaccatcc tcaaaggctt
    11941 taaatacagc tggaccgatt ccaaagaaat ccctcccatc atcatcatcg tggatgactt
    12001 tggctatgcc gataaagacc tcctgcaagg ctacgccgat ctgcccagag aagtggcttt
    12061 cgccgttctg ccagatctgc ccgccacgaa aaaggcagcc cagatcgctc agcggacggg
    12121 gcacgacgtc ctcatccacg cgcccatgga agccaaacaa tccaagctga aaccgggaga
    12181 acgctatatc aagactggcc aggatgctgg caccatcgcc tcgctcatcg acgccttcca
    12241 tgagcaaatg cccaatgcca tcgccctcaa caaccacatg ggcagcaccg ccacctccga
    12301 taaaaccgtg atgaaggccg tgctcgaaca cctggcccgc agaggcctct tctttgtgga
    12361 tagcgtcacc agcggtgaaa ccgtgggtta taacatggcc aacactttgg gccagagggc
    12421 tgtgcgccgc aatctctttc tggacgtgcc ggacaacagt gatgccaccc tcgcggccaa
    12481 aatctcggac ctgggaagat acaagggcag agtggagccc gtggtcgtga tcacccactg
    12541 ccacaatcag gccaaactcg atgccctcaa caagttcatt gcccagatca aggctatggg
    12601 gctgaggctc acatcccttt ccgcgtacta cggtggggga gtggcgctga actgaccggc
    12661 agggcttaca gggttttagg atttggagtt tagggctgct cggccgcgat tacagagctc
    12721 caagccactc tggccactcc tccaatgtgt ccccacgttg agtttccgtg tccgcggttt
    12781 cctattctca ccattgcatt gctcaaagta cagcatagta aacatagagt ttggttgggg
    12841 gccatttttc tctaatcgga tgggttgtga aatcctgagc attaaactcc aagagggagg
    12901 acactccctt ggtcaatcac acgttcaaac aatcactcga aaaaagcatt gacttcaatc
    12961 agggcttgaa atctttggta aagttgggaa acgatctaat gaaaaaagtt atcattgcca
    13021 tagacggtcc ggcggcgtcc ggaaaaagca ccacggcgag gctattggcc cagcgattgg
    13081 gctatgtgta tctcgatacg ggggctatgt atcgtgcctg cgctttgcgg gcttaccgtc
    13141 ttgggatcgc tgtcgatgat gtggaacgtg ttgccgcaat ggtggatacg atcgttctgc
    13201 ggattgattt tacggcaggc gtgaaccgcg ttttcctgga tggtgaagac gtcagcgagc
    13261 agatccgcac ccctgagatt tccgaattgg cctccgcgat atcggccttg cctccagtgc
    13321 ggcaaaagat ggtggaattg caacgtcgca tcgctcaggg aggtggattc atcctcgatg
    13381 gccgcgatat aggcagctat gtgtttccca atgccgagat taagtttttt ttgaccgcgg
    13441 accccgacga gcgggccaga cgcaggcagc tggaattggc agccaaaggc gtgaaagcca
    13501 gcctcgaaga ggtccgagcc gatcttttac aacgtgatat gaatgaccgc agccgcgctt
    13561 tggcacccct ggtgcaggca tccgacgcga tcctgatcga caccactggc ctcagcatcg
    13621 atgcccaggt ggagaagctg cgccagatag cgctccagag gttggaaacg ctgtgaatat
    13681 ctggctggca gagcattccg gattttgctt tggagtccgc cgcgccatcc agatggccag
    13741 agaagccgcc tcagttggaa agcccgtctg cacgatggga gagcttatcc acaatcccat
    13801 catcgttgat cagctcaaga gcgaggggat agacgtctgc gaagacccat tctcgcttcg
    13861 cgaccatacg gtgatcctca gatcgcacgg cgtcaccaaa gacatgatgg agactctggc
    13921 ccgcaacggc aaccggcttg tggacgccac ctgtccttat gtgaggcgcg cccaacaact
    13981 ggtggaaagc atgtctgaag cgggctacca ggtgatcatc atgggcaacg ctgaccatcc
    14041 tgaggtgatt ggcatgctct cctgggggaa cgcttccacc caagtgctga aagcggatga
    14101 ccggcccgaa ctcactggag gacaacagct ttgcctgatc agccagacca ccgaaaagcc
    14161 ggaacatctg gccgatatgg tagcgtggct cgctcccaga gcgaacgagc tgagggtctt
    14221 caacactatc tgcctggcca ctcaacagcg tcaggaagcc tcagaacgcc ttgcccgcaa
    14281 gctggacctc gtgattgtga ttggcggctt aaaaagctcc aacacgaggc agcttcacca
    14341 gctctgctct caatacagcc gctgctttca gatcgaaagc gaagcggacc tgcaagcgga
    14401 atggtttcaa gaatttaact cgattggcct ggcagccgga gcatcgactc ccgccgagac
    14461 aatcgtggca gtttataacc agatcaaaga aataaatggg gaacccgaca gggtgactgc
    14521 tgtcggggaa attcccttgt ttaaggagga atcatgttaa ctcatgatca aaacgaagtc
    14581 aaaaccgacc aaaccgaaat tgacctgatg gccggcgcag accagacgtc gcagacaaag
    14641 gaagaagagc ggattccctc acaggaagct accgcggaag ttgtgaaccc agaacttgaa
    14701 cctcaggaag ccgaagcgag cgctactgaa gaagctccag ctcctgaacc aatcgaagaa
    14761 ccagtagtcc cagccgctga acccgaagtg gaaccagtgg ccgaggaagc cgttccagaa
    14821 ccggaagcag ctgaagaacc agctccggtc gaggaaatcg tgccagaagc tcccaagccg
    14881 gcccccaaac ccaaagccga gcttagccag gaggaaaagg aacacgaaga gctgctcaag
    14941 atgtatgatc aatacacaga cagctttgaa agcggccaaa tcaaagaagg gaccgtagta
    15001 ggcgttacag acaaggatgt ggtggtcgcg ataggctaca agtccgatgg cgtgatcccg
    15061 atcagcgaat ttgcctacag cggtattcct gagctgaatt ccaccatcat ggtctttatc
    15121 aacaaggtgg aaaacggcga aggcaagctg gaactctcca aaaagaaagc ggatttcatc
    15181 cacaacatcg aaaagatcaa gcactccttc acggacggaa ctatcctcca gggtgtggtg
    15241 cgccgcagag tcaagggcgg catgatcgta gatgtgatgg gaatcgaagc ctttttgccg
    15301 ggctcccaga tgtctctcaa acctatcccc aatctcgatc aattcatcgg caaggaactc
    15361 agcttcaagg ttgtgagcct cgatgaagat caccgcaaca tcatcctctc ccgtaaacag
    15421 gtattggaag ccgaggccgc catcaaaaga gcggacctgc tggaaaagat tcagatcggc
    15481 tctgaactgg atggggaagt gaagaatata actcaatatg gcgcctttat cgatctgggc
    15541 gggatcgacg gcctgctcca cctcactgat atgagctggg gcaaggtgaa tcacccctcc
    15601 gaaatgctca gtatcggcga caaggtcaag gtgaaggtca tcaacttcga taccgaaacc
    15661 caaaagatct ccctgggact caaacagctc gttccgcatc cctgggaaac gatcgaactc
    15721 aaattccccg aagggacccg catcaccggt aaagtggtga gcgtgaagtc tttcggcgct
    15781 tttgtggaaa tcgagcccgg cgtggaaggt ctggtgcaca tctccgagat gtcgtggacc
    15841 aaaaagatcg ccgatgcccg taagatcctc aagattggtg attcgatcaa cgccatcgtg
    15901 ctggaactgg ataaagaaaa caagcgtatt tccctgggta tgaagcagat ggaccccaac
    15961 ccctggctga ccatcgaaga ccgctatccc atcggcaccg tcctcacccg caaagtgaag
    16021 agcctcacgg ctttcggagc tttcgtggag atcgaggaag gcatcgacgg cttgatccac
    16081 atttcagata tcagctggac caagcgcgtc taccaccccc gcgaagtcta tcgcaagggc
    16141 caggaagtgg aagctgtgat cctctcaatc gatcgcgctc tccatcgcat cgccttgggc
    16201 gtcaagcagt tgagccccga tccttgggaa aacctcaaca ttatactccc cgtcaacacc
    16261 gaggtcaaag gcaaagtcag caagctcatc cccaaaggcg tgctggtgga catccaactg
    16321 gataacgatg tggtggaagg gtttatcccc atctcccatc tggccattcc caagctggag
    16381 cactccgaag aagccttctt cgtgggtgaa gtcctgcccc tcaaagtcat cgaactcgat
    16441 atggacaacc gcaggctgat cctctctgtg aaagccttct tcttctcccg cgatcccaag
    16501 ctgcaggaag aatacatcgc catccacgaa cagtatatcc gcgaccgcat cgccaaaaac
    16561 caaaagaaga aagccgaaaa gcagagccgt gagaaacaga actccgacaa agccaaaacc
    16621 gatcagaacg atgaaggcgg cgtggcggaa gaagtcatga tcgaagctga aactcaggct
    16681 gatcacgtgg aaactgctcc cgctcttgaa gagcnnnnnn nnnnnnnnnn nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn nnnnatcgaa gctgaaactc aggctgatca cgtggaaact gctcccgctc
    16861 ttgaagagcg ggtaactgaa gcctattccg aagaaccgat agccgaagaa gtggtggaaa
    16921 cccttgctga agaaccggta gtagaagaag tagtggaagc tcccatcgaa gcacccgtag
    16981 tggaagaagt agtggaagct cacatcgaag ctccggtagt ggaagaagtg gtggaaaccc
    17041 ttgctgaaga accggttgtg gaagaagggg tggaaaccat cgtgaccgaa acggaagcca
    17101 ctctggaaga taccgagacc aaaccagtat aaaccatata tgtcagagga cattaaaaac
    17161 gcgatgacgg cccctcaagg ccgtcatcct ttattttggc tggatttgct gttgccggtg
    17221 ctgctgctgg ctctactgac gcccctgttt catatctttg attgggatat ggagttacaa
    17281 cgctggtggt tccactccga caccggttgg gaaaccggag agctgggcat ctttcagctg
    17341 ctctaccgct ttggcaatct tccagcgctg attctgtctc tgggagccgt atttgtattg
    17401 attcgcggtt tcagttccac ccgcctgagc tcctggcgca agatggcaat ctatctgatc
    17461 gcggtgatga tcatcggtcc gggattgttg gtaaacacct tgctgaaaga cagatggggg
    17521 aggccccgtc cgcgggatgt ggtggaattt ggcggcaaat atgcctatga agcacccctg
    17581 cagatcgatc ccaccagtcc cggcaaatcg tttccctgtg gacatgccac catgggattc
    17641 ttctttttcg ctccggcctt tctgctcaga aaatgggggc gcaagagctc ccgttcaagc
    17701 cttgccagct cgtcttacag cctcgcttta gcatggtttg ccggcttgtt tggaatcctg
    17761 atcggcattg ccagaatggc gcagggcggt cattttgcca gtgatgtggt gtgggcggga
    17821 acgctcgtct ggatagtggc ggcgggcttg tattatttga tgaggctgga tgtggcaccc
    17881 ttctccagac cacgcagcaa aggcaattcc acccttaaaa ccggttggaa actgctgttc
    17941 tggctgctgg gactcggctt gatcgccgga gtgggagtgg cgacgcccta tcaatcgcgc
    18001 cggggataca a
//
LOCUS       DAOSFN010000039        17548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2638_length_17548_cov_8.602470, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000039 DAOSFN010000000
VERSION     DAOSFN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_17548_cov_8.602470"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLO35_04045"
     CDS             complement(<1..932)
                     /locus_tag="PLO35_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HOH78868.1"
                     /translation="MRCKCVVVMVMLLVLTGLMGTVSNRVIQVSECSSTEVRLSLTDL
                     TPEFKQEVLGGRSYNSPSLEGAEYSATEACPSLPLYSGYVAIPAQGDYEISYSYKSYK
                     TYPGVTPKPASGDDKTTPAPSAQVYEGQSAWPEDIVSFGESAWIRDFRVLPMNVNPMQ
                     YDPVSGSIRHYQGIEIRLRFEADAGPSLGYQNYSSEFDKIYEANILNFDNLRALIVAP
                     LQARILIVYGNSTDATFQAKLAEFVAWKRQKGFEVNTASTVQTGGSSTNAIKNYLQGQ
                     YNNLDTRPDYVIIIGDANGTYAIPAYFETESGYNG"
     gene            complement(993..2018)
                     /locus_tag="PLO35_04050"
     CDS             complement(993..2018)
                     /locus_tag="PLO35_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78869.1"
                     /translation="MPRKVLLLCALIGLILIGGCAKRNTAWQEPSILSFVREIPVIGN
                     PADISFDADNLYISLDQGGIMMVNTANWQTNWWTQVLPGGADAQLINVRRASLVPQHK
                     LLFLGEFVAADKIRIVDVSDPDSLRLIESITGGTDGLSQIFFKAIPNPTDANIIEGFF
                     GAGRSLHYCRFNGSLYLGTTWSIEAPATVSGFDTTDQLVVIAAQQRGLAIYNRANQQK
                     ISEIAVPGEALAVKVSGNYAYVACRQGGFYIVDITNPATPVLKDGYDTTGYATSVDLH
                     DNLALVSSGGGGVYVFDVSNPNSIVLKEALTSAGYTNNAIFYEDQVLVSSRDNGVQIY
                     SLDLPGR"
     gene            2261..3541
                     /locus_tag="PLO35_04055"
     CDS             2261..3541
                     /locus_tag="PLO35_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOH78870.1"
                     /translation="MRIAKYAKILILFTLVLSGCGKNVTLYNQAKKHYKAGEFESALE
                     SSAQSLQLKPGYLKAQDLLKEVYPRALKLRMDNVNRLKAANDALMWDKLVPEYEALIK
                     IQTTMSGLPRLVHPKTGEVFMYDPIDYNPQLKESRENAAEYHYQKGLQLSRQSEAPEI
                     QKEAAKEFKLAVEFVSGYKDSLARYEQARKKAVKRIAILAFEDVTGTKSRFGGLPDML
                     VDNIVGNLINDKASAEFLEIISRAQIDAVLQEQQLSASGLVDESSTANLGQLLGAHEI
                     LSGKILQIDSVPARTTSVQLKESATIEIEQGEAESDPESEEETEPVKIKQDVQCLYNK
                     FTKTASARITASYTIVDVSSGKIKIQNTYTGVFEWSETWARKESGDDRALTPATKALI
                     AKAEPFPPSDSQIVNNALKDLSDQFIKQIKGYVR"
     gene            3531..4898
                     /locus_tag="PLO35_04060"
     CDS             3531..4898
                     /locus_tag="PLO35_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78871.1"
                     /translation="MSDSSNRLVLLLSLGFLLFSCAANSKTPVWVKKEPTDPLFYSTV
                     VKVSKQGTLYKERAFETALKNISMQITVTVDASVTTRETEAFGLTASDFNSSIQTSSR
                     AQLSGVELAGSHETKKEYWAWYRLNKEAFRSARQMQCDTAVRTSLDQLSKFDAALQEA
                     SVDFSISAGFLIFALDQLSDFLDLDLRATYEGKEIQLYSELVHRVAELSAKTRISLDP
                     TRMTAVARRPSDLRSKIDCRYAHKESGQAVTALPLLAQFSKGTGDLSANLTSNQFGTA
                     ELIITRVTAFDASQLVGVKVDKELLIGMSGHPLVRKLLKGLNFSSASLELEVRRPRVW
                     VSSQTNQQENASVPLIEDRLRELEVEVTQEQGNSDYILRVEIESNPGSYIPNLKLYSA
                     QSNARLSLIDAVTGTTLGSESLISVKGTGPNPETAIRNSEQTSLKAISQDTLYLLISN
                     HIIGF"
     gene            complement(5093..6019)
                     /locus_tag="PLO35_04065"
     CDS             complement(5093..6019)
                     /locus_tag="PLO35_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOH78872.1"
                     /translation="MKRLAYTLTLILVVALFSGCGTNTDLISENPEDLIRYGTDDTFE
                     VLTWNLREFPLQSTQTLEQLAHIIPLMNVDLLAFQEINDQASFLELARMIPHYSAYVS
                     SATSSYRLAYLYDSRTVTVNDDYTIFNEDSNPFPRPPYVLDISWQGVDLLVVNNHLKA
                     FGDNYIDENDSWDEEYRRRLACQKLDQHIRSNWSDERVIVVGDMNDQIAEPVEYNVFN
                     AFLDRPAEYLFADMDIAQNPSPANVSYPPSFSHIDHILLTNELFDSFADLDSSCDTLP
                     VESLLGTWENYSNQLSDHRPVALKLKFPGLSD"
     gene            6170..9652
                     /locus_tag="PLO35_04070"
     CDS             6170..9652
                     /locus_tag="PLO35_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH78873.1"
                     /translation="MKLHIVLILLLALSLLPATVTERFDGTPQSIRGSISLSRQAPVA
                     QDQILDEEGIDSVTAFSRTFALPVQDVTLSVHSITWNVFDSQGNFLYQQSNRDQNSVV
                     VANRFQFREMRGFTITAKTQLADGDLMLGLAELDFSLSGQGSIPLPSSVSPAFIDAYK
                     ALADNWDTCYLRDLPVARPKMLIISHPNLTNYQTDFINWKRALGYDVMVVNRVDIGSS
                     VNEIKSYLVNHYQQHHCDYLLLLGDVSGSYSIPTAFFPSPEYAENDADDHQYTLIEGD
                     DYFPEMLVGRFSFNDIAEFMTMANKTVVYEKTPFMGNTNWMTRSLAVAGNYAEGGLRP
                     TTPVHMSRWLRDKMLTHGYAQVDTVFYPPTYPGTTSILSSINQGVQFISYRGWGDANG
                     WHYPSFHNPDLNSVLSYQRMPIVFSIVCNTGDFANSVNPCFGEKWMRMGSVSQPNGCI
                     AFVGPSDLHTKTRLNNSISSGAFRSILDNGVRGFGSSVLIGKIELYKNFPNDLAPDQY
                     VAFYYHVYNLLSDPSLNMWVLVPQTISQDLLPAEYLASDSHISFSAAHLNGAIVSGTK
                     NNTAFTYARVVDGVAYLPINPEEEGDLTITVSKPNFVPLVKTVTRVGDLGIGIIDNSV
                     LDTFLNPNQTVNGTIALRNYGSSEVVITNAVIGGSDKVSVNYTHSQFSILPGAIAQIS
                     FQLIGSANIIPREALTLNLQINSLPPKYFRLWGGGAEFTVVSTTGTYPIGQTSQVGFT
                     IMNTSGASLTNASVQVVSLTEAATFPAAPITLGSIGAGESKTFNASITVGANAWNGRH
                     LPLKLIVTDAGYSTQTFYSLTAGIAATTSPTGPCTYGYYAYDSFDLGFPSTPVYEWVE
                     IDPLLGGNATVFLNQDDGSRVVDLPFVFRFYGRDYDQITICSNGWISMGATDMFDFYN
                     HYIPAALGPYSMIAGYWDDLKGEQTGVDGDGNPVFADMRMLYWHDVANNRYIVQWNDA
                     YNQYNLTSLEKFQIILYPRAGMDGDIVVQYHTIDNPGMTTNYCTVGIENHNQLVGLTY
                     THGNVYPPTATPLQAGLAVRFTTTAPDTYVSNDDSVQSPVISLAQNFPNPFNPSTTIS
                     FELKRPSPASLSIYNMKGQLVRNLVNTDLNAGKHSFVWNGTNDNGQAVASGLYLYRLH
                     ADGYRAERKMLLMK"
     gene            9815..12130
                     /locus_tag="PLO35_04075"
     CDS             9815..12130
                     /locus_tag="PLO35_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78874.1"
                     /translation="MKRSLSLLLSCLILITLNATAREDYIIGAYSQYQLHHAHQTEAV
                     FSDLAELLKGAGYNTMLYSTRYEEAVNGRLELALTALNRYGIGSIIDDWAWQTDGPVG
                     VTAMANGNYLKMEAEYKWDHQGSKYSPDSFHNDRSDNDYYNIVFRHETGRRSPHQPER
                     FSNSYAWICDASVGDKAGVAASEPRFRWKPESRSFSRVLGYDLKFFPDAKDNRLYLKV
                     ALNWQDLPLDAKVAQISIKALKNAQMSSRDGVYGVYPEDAYQELELFSSQPDVYSTTI
                     FNQNYSGVDKDPLSGALIFEYWTPIPAPSSKLYTEVMSFDFFNHISPTVHWYGEGRLE
                     IDYLELEDELYRALRQAKHPLQDRLAARLEQIDKVPGSESILFFYGKDEPFQGQFATY
                     ARLEDFLKARDRGLITATHLENTFYKKPGGLPDYFHFGLFLQEGKPSVVMLDAYPLQE
                     WGPGPGARIRWNNDFDHELFVQNKIQSVVLANYHKLTSSVKHSADHSNTRLFYVPQSF
                     GEKYEPLETGEWRYFMPPLSMQKCLQLLPLCYAADGVIDFAITSNRDQGFPTGSRAFR
                     RVTPISHKANYLQPGSPDGNSYLRNLAEANAKIAVYGPIIRTLKWLDADSLMVNGSHP
                     KLDIKALNLKSLEVLSPANGPYEGYVQCGYYQDTNGLPAFMLVNRRAVYRSDGDGLVS
                     WDVEKHFVDAEPQTVKLELDPDQLKGKRYGLYDPFLNHLIISDDNTYEIEIAAGDGIM
                     LQLVSLPEDEQKQAPKPARRRSWIKRLFGIK"
     gene            12270..>12718
                     /locus_tag="PLO35_04080"
     CDS             12270..>12718
                     /locus_tag="PLO35_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH78875.1"
                     /translation="MKNILILGAAGQIGSELVPYLRNIYGDSHVVASDLHANVEAGLK
                     ASGPYEILDALDGSAMDALVRKYKVDTVFNLVAVLSAKGEANPVMAWKINMGALFNAL
                     EISRATHGAVFTPSSIGAFGSTTPMDKTPQDTVMRPSTVYGISKVAA"
     assembly_gap    12719..12818
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12819..13046
                     /locus_tag="PLO35_04085"
     CDS             <12819..13046
                     /locus_tag="PLO35_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOH78876.1"
                     /translation="AMSFDPEMITAAIRKHLPDFKLTYNVDPIKQAIANSWPNSMDDS
                     SARAEWGWKHDYDLDAMTADMLDKLRVRLAI"
     gene            13054..14043
                     /locus_tag="PLO35_04090"
     CDS             13054..14043
                     /locus_tag="PLO35_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOH78877.1"
                     /translation="MLKIAVVGVGHLGKHHARKFAAMPDVLLAGIYDQDTQRCQELAQ
                     ELEVKAFGNYQALLDACDGVDISATTTAHYDLAKTALLAGKHVFVEKPITAELGQARE
                     LVSLAAQQNLKLQVGHIERFNPVILAVEDEIANPMFIESHRLSTFHHRGTDVPVVLDL
                     MIHDIDLILDFVKSPVKDIRASGVGILTPSIDIANARLEFENGAIANVTSSRVSMKQE
                     RKIRFFQRDAYFSLDFGAKHATVMKRSPDILKYLPQILAGSTDIKAEQLVDVKHHDLS
                     LEAKDALNMELESFVTCIVKDKPPIVDGVAGTRALEVALRILEIIGHQPKTHI"
     gene            14054..15349
                     /gene="asnS"
                     /locus_tag="PLO35_04095"
     CDS             14054..15349
                     /gene="asnS"
                     /locus_tag="PLO35_04095"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOH78878.1"
                     /translation="MSYIQIKDIPQHLEREISMRGWVRNIRHSGKLLFIILRDGSGEI
                     QCVAFKPELGDEKFELARKLTMESSVIISGIARPHQKMQDQYELAVTDLMPVQIADEY
                     PISKKEHGPDFLLSNRHLWLRAPKQWALMRLRHTVYFAICDYLNQNDFFRFDSPILTP
                     NACEGTTTLFELDYFDEGKAYLSQSGQLYLETGIMSLGRVYDFGPVFRAERSKTRKHL
                     TEFWMMDAEAAWVEHEENMAIQEGLIRFVIRTVLERCDRELGILERDKEALKAADAPF
                     RRITHYEAIEYLRSKGSEITHGSDFGAAEEALLTEGSPVPVFVEKWPKEIKAFYMKRD
                     PENPNLVLGSDLIAPEGFGEIIGGSQREDDHELLLGRMKDEKMPIDDYQWFLDLRKYG
                     SVPHSGFGVGLERLVTWMSGIHHIRETIPFPRMIYRIYP"
     gene            15576..16787
                     /locus_tag="PLO35_04100"
     CDS             15576..16787
                     /locus_tag="PLO35_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOH78879.1"
                     /translation="MNYASDFSRVTQTMKSSLIRELVAATKSIPGLISFAGGFPSPAT
                     FPKQILAELYREVITQDGLDVLQYGASDGDAVLKRELVKWEGYDIAANQMMTTVGATN
                     AIYYYALSLLDPGDVVICEAPTFLGTLVALDALQAKLVSVPIDNDGISLEHLEAAVRS
                     CKAAGKKIKFIYTIPDFHNPGGVTMSMQRRRDLLNFAIEHQLPILEDNPYSRLRFDGK
                     PLPTLYRLMRQEFTGSEIVTEVVSFSKILGPGMRIAFVKGEASLLQKMESWQQKVNVT
                     PDCVSQRVAARFLADGFMEPHLQSVCEHYKPYLRKMLDCLEAYMPKGVSWTRPQGGIF
                     VWMELPEHQNADEIFHAAAQNKVSFIPGSKFYPAGQERFNTLRLNFTYSSLDQIDTGI
                     QRLAELLKALA"
     gene            16887..17330
                     /locus_tag="PLO35_04105"
     CDS             16887..17330
                     /locus_tag="PLO35_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78880.1"
                     /translation="MNKLAITLIALAVACLPLAAQIDLPKPDFSLMPRGGRSFLDTNK
                     LSMSHSMGFEAGSSSYGGYYLSRYTNHLKYSFNPKLDLQVDLSLINFGSASPAFKIND
                     DNSSRFVPGFTLDYHPSESFKLQVKYQQGYPMWGTIKPWYELERP"
     gene            17358..>17548
                     /locus_tag="PLO35_04110"
     CDS             17358..>17548
                     /locus_tag="PLO35_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOH78881.1"
                     /translation="MIILLAALGASLGSFFNVLIHRLPQGKSIIKPASSCAGCGYRIP
                     FYLNIPILSYIILKGRCKQ"
BASE COUNT         4321 a         4714 c         4363 g         4050 t
ORIGIN      
        1 ccgttgtaac cggattcggt ctcaaaatag gctgggatgg cataagtgcc attggcgtct
       61 ccgattatga tcacgtaatc tggccgggta tcgagattgt tgtattggcc ctggaggtag
      121 tttttgatgg cgtttgtgga ggagccgccc gtctggaccg tgctggcagt gttgacttca
      181 aaaccctttt gccttttcca cgccacaaac tctgccagtt tggcctggaa ggtggcgtct
      241 gtggaattgc cgtagacaat caatatccgg gcctggagag gggcgactat caaagccctg
      301 aggttgtcga aattcaggat gttggcttca tagattttgt caaactctga gctgtagttt
      361 tgatatccga ggctggggcc cgcgtcagcc tcgaacctga gtctgatctc aatgccctgg
      421 tagtgtctga tgcttccgct tacgggatcg tattgcatcg gattgacatt catgggcagg
      481 actctgaaat ccctgatcca ggcggactcg ccaaagctta cgatatcctc aggccacgcg
      541 ctctgtcctt catatacttg cgctgagggg gcaggagtgg ttttatcatc accgctggcg
      601 ggtttaggtg taacgccggg ataagtttta tatgacttgt agctgtagct gatctcatag
      661 tcgccttgcg ctggaatcgc gacgtaacca gagtaaagtg gcaaggaagg acaggcttcc
      721 gtggcggaat attccgcccc ttccagagag gggctattgt aacttctgcc ccccagaact
      781 tcttgtttaa actcaggggt caagtccgtc aaactcagac gaacttccgt agatgaacac
      841 tctgacactt ggatgacccg attggagacg gtccccatca gtcccgtcaa caccagcagc
      901 atcaccatca cgacaacaca cttacatctc attttttcct ctttatacgc aaaaggacct
      961 gaagctaact tcaggtcccc gataatgaca gtttatcggc ctggtaaatc caggctatag
     1021 atttgcactc cgttgtctct ggaggataca agcacctgat cttcatagaa gatcgcgttg
     1081 ttggtatatc cggccgaagt gagagcttcc ttgagcacta tgctgttggg attggaaacg
     1141 tcaaacacat acactccccc tcctcccgag cttacaagcg ccagattatc atggagatcc
     1201 acgctggtgg cgtaaccggt ggtatcgtat ccgtccttca aaaccggagt ggcgggattt
     1261 gtgatatcca ctatataaaa accaccctgg cggcaggcaa cataagcgta gtttccggat
     1321 actttgacgg ccagagcttc ccccgggacc gcaatctcgc tgatcttttg ctgattggcg
     1381 cggttgtaga ttgccagtcc acgttgctgg gctgcgatca ccacgagctg gtcggtggta
     1441 tcaaagccgg acaccgtggc gggggcctca atcgaccaag tggtgccaag atataacgat
     1501 ccgttaaagc ggcaatagtg caaagagcgt ccagctccaa agaaaccttc aatgatgttg
     1561 gcgtccgtgg gattgggaat cgctttgaag aatatctgac tcaggccgtc ggtcccacca
     1621 gtgatggatt cgatcagacg cagtgagtcc ggatcgctga catccactat gcgaatttta
     1681 tcagccgcga caaattctcc caggaacagc agcttgtgct gcggaaccaa tgacgccctg
     1741 cgaacgttta tcagctgcgc gtcagctcct cctggcagca cctgggtcca ccagttggtc
     1801 tgccagtttg ccgtgttcac catcattatg ccgccctgat ccagagagat atacaggtta
     1861 tccgcgtcaa agctgatgtc ggcaggattt ccgatcacgg ggatttctct tacaaagctg
     1921 aggatcgatg gttcctgcca ggcggtgttg cgtttggcac agccgccgat caggattaac
     1981 cctatcagtg cgcatagtaa caggactttt ctgggcattt ccattctcct tgaacagatg
     2041 aaattaatgg gcccccttaa cagagaccgc ctgatcgcag ccaggctgat gtaacttgag
     2101 caatatctgt tccgataaac agaaattatt atccaggccc aaggaaagaa accacacgtt
     2161 gaagctccag actgacggat cttgcgaaaa ctgcttgaca ctctgctccc cagtacatct
     2221 ttgggaaaaa aacataaaac cttatcttta tggaggaacg atgcggatcg ccaaatatgc
     2281 caagatcctg atcctgttta ctttggtcct ttccgggtgc ggaaagaacg tcacgcttta
     2341 caatcaggcc aaaaagcact acaaagcggg tgagtttgaa tccgcgcttg agtccagcgc
     2401 tcagtctctg cagttaaaac ccggatactt gaaggctcag gacctgctga aagaagtgta
     2461 tcccagagct ttgaaattga gaatggacaa tgtcaacagg ttgaaagctg ccaatgatgc
     2521 cctgatgtgg gacaaacttg tgcctgaata tgaagccctg atcaagattc agaccactat
     2581 gtccggtttg ccgagactgg tgcatcctaa aactggcgag gtgtttatgt acgatcctat
     2641 cgattacaac cctcagctca aagaaagccg tgaaaacgcc gccgagtatc attatcaaaa
     2701 gggcttgcag ctttcacgtc aatcagaagc tccggagatt cagaaagaag ccgccaagga
     2761 gttcaagctc gcggttgaat tcgtttccgg atacaaggac agtctggccc gttatgagca
     2821 agcccgcaag aaagctgtga aacggatcgc gatcctggca tttgaagatg tgacaggcac
     2881 aaagagccgc tttggcgggc ttccggacat gctggtggat aacattgtcg gcaacctgat
     2941 caatgacaaa gcctcagccg aatttctgga gatcatcagc cgcgctcaga tcgacgccgt
     3001 tcttcaggaa cagcagctca gcgcttccgg cctggtcgat gaaagcagca ccgcgaacct
     3061 gggtcagctg ttgggagccc atgaaatctt gtctggaaaa atcctccaga tcgattccgt
     3121 gcctgccagg accacttctg tgcagctcaa agaaagcgcc accatcgaga tcgagcaagg
     3181 cgaggcggaa tcagatcccg aatccgagga agaaaccgag cccgtcaaga tcaagcagga
     3241 cgtgcagtgc ctctacaaca agttcaccaa aaccgcctca gccaggatca cagcctccta
     3301 cacgattgtg gacgtgagca gcggtaagat aaaaatccag aacacttata ccggcgtctt
     3361 tgaatggagc gaaacctggg ctagaaaaga gagtggagac gaccgcgctc tcactcccgc
     3421 caccaaagct ttaatcgcca aggctgaacc gttccccccg tctgatagcc agatcgtcaa
     3481 caacgcactg aaagacctca gcgatcagtt tatcaaacag attaaaggct atgtcagata
     3541 gctctaaccg tctcgttctt ttgctcagcc tggggttttt gctgttttcc tgcgccgcca
     3601 acagcaaaac ccccgtctgg gttaagaaag aacctaccga tcccctcttc tactccaccg
     3661 tcgtcaaggt gtcaaaacag ggtaccttat ataaggaaag ggcttttgaa actgccctga
     3721 aaaacatctc catgcaaatc accgtgacgg tggacgcatc agtaacaacc cgcgagaccg
     3781 aagctttcgg attgacggcc tcggacttca attcgagcat ccagaccagc tcccgggcgc
     3841 aactgagcgg agtggaactg gccggcagcc atgagaccaa aaaggaatac tgggcctggt
     3901 acagactcaa caaagaagcc tttcggtctg cccgtcagat gcaatgtgac acggctgtcc
     3961 gcacttccct tgatcaactg agcaaattcg atgccgcgct gcaagaggcc agcgtagatt
     4021 ttagcatctc cgcgggtttt ctgatcttcg ccctggacca gctcagcgac tttctggacc
     4081 tggacctgcg cgccacctat gaaggcaaag agattcagct ctattccgag ctggttcacc
     4141 gcgtggcgga gctttctgcc aaaaccagaa tctcgctcga tcccaccaga atgacagctg
     4201 tggccagacg gccctctgat ttgaggtcaa agatcgactg ccggtatgcc cataaagaat
     4261 ccggtcaagc cgtgaccgcc ctcccgctcc tcgctcaatt cagcaaaggt acaggcgacc
     4321 tgagtgccaa tctgacttcc aaccaattcg gaaccgcgga actgatcatc actcgtgtga
     4381 ccgcttttga cgcatcacaa ctggtaggcg tcaaagtgga caaagagctc ctgatcggga
     4441 tgagcggtca tcctctggtc cgaaagttgc taaaaggcct gaatttcagc tctgccagcc
     4501 tggaattgga agtgaggcgt ccgcgcgtgt gggtatccag ccagacaaac cagcaggaga
     4561 atgccagtgt gcctctgatt gaagaccggc ttcgcgagct ggaagtggaa gtgacccagg
     4621 aacaaggcaa tagcgattac atactgagag tggagatcga atctaatccc ggcagctaca
     4681 tccccaatct gaagctatat tccgctcaaa gcaatgccag attgagtctt atcgatgcag
     4741 tgacgggaac cacgctggga tcggaatccc tgataagtgt caaaggtacg gggccaaatc
     4801 cggaaacggc catcagaaac tccgaacaga ccagcctcaa agccatcagc caggacaccc
     4861 tctacttgtt gatatccaat cacattatcg gtttctaagc cggatagcta tccccagacg
     4921 ctgtactccc ttatggctca gcgtctcttt tttatcaggg ttttattacc ttggctcaga
     4981 gccaggatgg cagcccccga aaacgatagg acgggtacag gtataccaag ccccacccaa
     5041 gccggacaaa tgcgctttgg gtccgggatg ctaccgccat gcccgctcta tcctaatcag
     5101 agaggccagg gaacttgagc ttgagagcca cgggacggtg atccgagagt tggtttgagt
     5161 agttttccca ggtgcccagt agactctcga ccgggagggt gtcacagctg ctatcgagat
     5221 cggcaaagct gtcgaatagc tcgttggtga gcaggatgtg gtcgatgtgt gaaaatgatg
     5281 gtggatacga gacgttagcc gggctgggat tctgcgcgat gtccatatcc gcgaagagat
     5341 actcggcggg gcggtccaga aacgcgttaa agacgttgta ttccaccggt tccgcgattt
     5401 gatcgttcat atctcccacc acgatcacgc gctcatcact ccaattgctc ctgatatgct
     5461 ggtccagttt ctgacaggcc agccgtctgc ggtattcctc atcccagctg tcattctcgt
     5521 ctatatagtt gtcaccaaag gccttgagat gattgttgac gaccagtaaa tctactccct
     5581 gccagctgat atcgagaacg tacgggggtc tgggaaaggg gttggaatct tcattgaaga
     5641 ttgtataatc atcgtttacg gttacagttc tggaatcata caggtatgcc agccgatagg
     5701 aactggtcgc gctggacacg taggcgctgt aatgagggat catcctggcc agttcaagaa
     5761 aggaggcctg gtcgttgatc tcctgaaagg ccagcaagtc aacgttcatc aagggaatga
     5821 tgtgggccag ctgttccaga gtctgagtgc tctgcagcgg aaactctctc agattccagg
     5881 taagcacttc aaaggtgtcg tcagtaccgt atctgatcag atcttccggg ttttcggaga
     5941 tgagatcggt attggtgccg cagccgctga aaagtgccac gaccaagatc agcgtgagtg
     6001 tataggcgag tcgtttcatt tatgttccag tagattgtta gtgcattatc tgcatgcaat
     6061 attttttcca attagatatt tataataggc gacgatattg attccgcgcc tggcagttaa
     6121 atcggaacga ttcttgcttt gagtagttaa tccgaaatag ggaggaataa tgaaacttca
     6181 tatagtactg atcttgttgc tggcgctaag cctgttgcca gccacggtca cagagcgctt
     6241 tgacggcact ccgcaaagca ttagaggcag tatcagcctt tccaggcaag ctcccgtggc
     6301 tcaggaccaa attctggacg aggaaggcat cgattccgtc acagcctttt caagaacttt
     6361 cgcgctgcct gtgcaagatg taactctgag cgtgcacagc atcacctgga atgtctttga
     6421 cagccaggga aacttcctct accagcagag caaccgcgat cagaacagcg tggtggtggc
     6481 aaaccgtttc cagttcagag aaatgcgggg tttcacgatc acagccaaga cccaactggc
     6541 ggacggcgat ctgatgctgg gcctggcgga gctggatttc agcctcagcg gtcagggcag
     6601 tatcccattg ccatcaagcg tatcaccagc ttttatagat gcttacaaag ccctggcaga
     6661 taactgggac acctgttatc tgcgagacct tccggttgcg cgacccaaaa tgctgatcat
     6721 cagtcacccc aatctgacta actatcagac cgatttcata aattggaaaa gggctctggg
     6781 ctatgatgtg atggtggtca accgggtgga cattggctca tccgtaaacg agataaaaag
     6841 ctatttggtt aatcattacc agcagcatca ctgcgattat ctgttgctgt tgggtgatgt
     6901 gagtggcagt tactccatcc ccaccgcctt cttcccttct ccggaatatg cggaaaacga
     6961 cgccgatgat catcagtaca cgctgatcga aggagacgac tactttcctg aaatgttggt
     7021 gggacgcttc tctttcaacg acatcgccga gtttatgacc atggccaaca agactgttgt
     7081 ctatgaaaag acccccttca tgggaaacac caactggatg accagatctc tggctgtagc
     7141 cggtaactat gctgaaggcg gcttgaggcc cactacccct gtgcatatgt cgcgctggtt
     7201 gcgggataag atgctcactc acggttacgc tcaggtggat accgtctttt atcctcccac
     7261 ttatccaggc accacttcga ttctcagttc catcaaccag ggcgtgcaat tcatcagcta
     7321 cagaggatgg ggagacgcca acggatggca ttatcccagt ttccacaatc ctgatctgaa
     7381 ctccgtgctc agctatcaga gaatgccgat cgtgttctca atcgtttgca acacaggcga
     7441 ctttgccaac tccgtaaacc cctgttttgg agaaaaatgg atgcggatgg gctccgtgtc
     7501 gcaacccaat ggttgcatag cgtttgtggg accgtcagac ctgcacacca agaccagatt
     7561 aaacaactcc atctccagtg gggcttttcg cagcattctg gataatgggg tgcggggatt
     7621 tggctccagc gttctgatcg gcaagatcga gctctataag aacttcccca acgatctggc
     7681 cccggatcaa tacgtggctt tctactatca tgtctataat ctgctctctg atcccagcct
     7741 caatatgtgg gtgctggtgc cgcagactat ctctcaggac ctcttgccgg ctgagtatct
     7801 ggcctccgac agccacatca gcttcagcgc cgcgcacctc aatggagcta tcgtttccgg
     7861 caccaaaaac aacactgcct tcacctatgc cagagttgtg gacggcgtcg cttacctgcc
     7921 gatcaatcct gaagaagaag gtgacctcac gatcactgta tccaaaccca atttcgtacc
     7981 gcttgtcaag actgtcacca gagtgggaga cttgggaatc ggaattatcg acaacagcgt
     8041 gctggatact ttcctcaatc ccaaccagac cgtcaatggc accatcgctc tgagaaacta
     8101 cggcagctct gaagtagtga tcacaaacgc tgtcatcggt ggttctgaca aagtaagcgt
     8161 gaattacact cactcgcagt tcagcatcct gcctggagcg attgctcaaa tcagcttcca
     8221 actcatcggc tccgccaata tcatcccacg tgaagcattg accctcaatc ttcagatcaa
     8281 cagcttgccc cccaagtatt tcaggctctg gggcggaggc gctgagttta ccgttgtaag
     8341 caccactggc acctacccga ttggccagac cagccaggtg ggattcacga tcatgaatac
     8401 cagcggcgcc tcactgacaa acgcctcagt gcaagtagtt tcgcttactg aagctgccac
     8461 tttcccggcc gcccccatca ctttaggaag catcggggcc ggagagtcca aaactttcaa
     8521 cgccagcatc actgtaggag ccaacgcatg gaacggcaga catttaccgc tcaagttgat
     8581 cgtcactgat gccggatact ccactcagac cttctattct ctgacggcag gaatcgccgc
     8641 taccacatca cccactggtc cctgcactta tggttattac gcctatgata gttttgatct
     8701 ggggtttcct tccacgccag tatatgaatg ggtggaaatc gatcccttgc tgggtggtaa
     8761 tgccacggtg tttctgaatc aggacgacgg ctcgagagtg gtggacctgc ccttcgtttt
     8821 caggttctac ggcagagatt atgaccagat caccatctgc tccaatggct ggatatccat
     8881 gggcgccacc gatatgttcg atttctacaa tcactatatc cccgccgccc tgggtcccta
     8941 ttccatgatc gcgggctact gggatgacct caagggtgag caaaccggcg tggacggtga
     9001 tggaaatccc gtctttgccg atatgagaat gctctactgg cacgatgtcg ccaataaccg
     9061 ttacatcgtg caatggaacg acgcctacaa ccaatacaac ctgacgtctt tggaaaaatt
     9121 ccagatcatc ctctacccca gggctggcat ggatggcgat attgtggtcc agtaccacac
     9181 tatagacaat cctggtatga ctaccaatta ctgcacggtg gggatcgaaa accacaatca
     9241 gttggtgggg ctcacctaca cccacggcaa cgtctatcct cccaccgcga cgcctctgca
     9301 agcaggcctg gcggtgcggt tcaccacaac cgcgcccgac acctatgttt ccaatgatga
     9361 ctcggtccaa agccccgtga tcagcctggc gcaaaacttc cccaatccct ttaatcccag
     9421 caccaccata tctttcgagc tcaaaagacc ctccccggcc agcttgagta tctacaatat
     9481 gaaaggtcag ctggtgcgca acctcgtcaa caccgatctc aacgccggca aacacagctt
     9541 tgtgtggaat ggcaccaatg ataatggaca ggcagtggcc tccgggcttt atctctatcg
     9601 ccttcatgcg gatggttata gagcagaacg caaaatgctg ctgatgaaat aattaagctc
     9661 agacaactct agctgaagcg gagatcggat tcggtctccg cttcactttt ggaagcagaa
     9721 aattgtctta actcctctct cctgcacttt tgccttgacg ctgagcgcta tccgcaattg
     9781 cgttgcactc aacaatatct cccaaggaaa catcatgaaa agatccctgt cactcctcct
     9841 ttcctgcctg atcctgataa ctttaaatgc caccgcaaga gaagattaca tcatcggtgc
     9901 atacagccag taccagttgc atcacgctca tcagaccgaa gcggtattct cagatctggc
     9961 tgaactgctc aagggggctg gctacaacac catgttgtat tccacccgct atgaagaagc
    10021 tgtcaatggg aggctggagc tcgctttaac agctttaaac cgctatggga taggttcgat
    10081 cattgacgac tgggcctggc agactgacgg cccggtgggt gtgaccgcca tggccaatgg
    10141 caactacctc aaaatggaag ccgaatacaa atgggaccat cagggcagta aatacagtcc
    10201 tgattcgttc cataatgaca gaagtgataa tgactactac aacatcgttt tccgtcatga
    10261 aaccgggaga cgctcacccc atcaaccaga gcgattttcc aattcctatg cctggatctg
    10321 tgacgcctcc gtgggagaca aagccggcgt cgctgccagc gagcccagat ttcgctggaa
    10381 gcctgaaagc cggtcgtttt ccagagtatt gggctatgat ctgaagtttt ttcccgatgc
    10441 taaagacaac cggctctacc tgaaagtagc gctgaactgg caagatcttc cgctggatgc
    10501 caaagtggct cagatcagca ttaaagcttt gaaaaacgcc cagatgagca gcagggatgg
    10561 cgtctatgga gtctatcctg aagatgcgta tcaggaactg gagttgtttt catcccagcc
    10621 agacgtttat agcaccacga tcttcaatca aaactatagc ggtgtggaca aagaccctct
    10681 cagcggagct ctgatctttg agtattggac tcccataccc gcgccatcat cgaagctgta
    10741 taccgaagtg atgagctttg actttttcaa ccacatctcc cccaccgttc attggtacgg
    10801 cgaaggccgc ctggaaatcg attatctgga actggaggat gagctctatc gcgccttgcg
    10861 gcaagcaaaa cacccgctgc aggacaggct ggcggcccgg ctggaacaga ttgacaaggt
    10921 cccgggatct gaatcaatcc tcttcttcta tggcaaagat gagccttttc aggggcaatt
    10981 tgccacctac gccagactcg aagattttct gaaagccaga gatcggggtt tgatcaccgc
    11041 gacgcacctg gaaaacacct tctataaaaa acctggagga ctgccagact actttcactt
    11101 tgggctcttt ttgcaggagg gaaagccttc ggtggtaatg ctggacgctt atcccctcca
    11161 ggaatgggga cccggaccgg gagccaggat tcgatggaac aatgattttg atcatgagct
    11221 ctttgtgcaa aacaagatcc agagcgtggt gctggccaat tatcacaagc tcaccagttc
    11281 tgttaaacac tcagccgatc attccaatac aaggctcttc tatgtgcctc agagctttgg
    11341 agagaagtat gaacctctcg agacgggaga atggcgctat tttatgcctc ccctcagcat
    11401 gcagaaatgc ctgcaactcc tgccgctttg ctatgccgcc gatggggtga tcgattttgc
    11461 cattacttcc aaccgcgatc agggctttcc gactggcagc cgggctttca gacgcgtcac
    11521 ccccatctct cacaaagcca actatctgca gcccggttct cccgatggca attcctatct
    11581 ccgcaacctg gctgaggcca atgccaaaat cgctgtttac ggtcctatca ttagaactct
    11641 caaatggctg gacgcggatt ccctgatggt gaatggaagc catcccaagc tggatatcaa
    11701 agccttgaac ctcaagagcc tggaagttct gagcccggct aacgggccat acgaaggcta
    11761 tgtacaatgc ggttactacc aggataccaa cggcctccca gcttttatgc tggtaaaccg
    11821 gcgagccgtt tacagatctg acggggacgg actggtttcc tgggatgtgg agaagcactt
    11881 tgtggatgcc gagcctcaaa ccgtgaaact ggagctggac ccagatcagc tcaaaggaaa
    11941 gcgctacggc ctctatgatc cctttctgaa ccacctaatc atctctgatg ataacactta
    12001 tgagattgag attgcggctg gagatggaat catgcttcaa ctggtaagct tgccggagga
    12061 tgaacaaaaa caagccccca aacctgccag gcggcggtcc tggataaaac gtctctttgg
    12121 catcaaataa tccccgcacc cgcctgaccg gatcagagcc tggaaaaggg tttccgatcc
    12181 gccccgcatc tataaaagca ttgacaaaag cggccgttca gaaagcgtgt gcgaaaaaaa
    12241 tatccacctt tttcagggag tgactcacga tgaagaacat cctcatctta ggcgcggccg
    12301 gacagatcgg ttccgaactg gtgccctatc tcagaaacat ctacggagac agccacgtag
    12361 tggcctcaga ccttcacgcc aatgtcgaag ccggcttgaa agcttcagga ccctatgaga
    12421 ttctggacgc tttggacggc agcgctatgg atgctttggt acgtaagtat aaggtagata
    12481 ccgtcttcaa tctggtagcg gtgctttcag ccaaaggtga agccaatccc gttatggcat
    12541 ggaaaattaa catgggagcc ctgtttaacg cccttgagat ttcccgcgcc acccatggtg
    12601 ccgtctttac gccatcgtcg attggagcat ttggatccac tacacccatg gacaaaactc
    12661 cccaggatac cgtgatgaga ccttccaccg tatatggcat ctccaaagtg gcggccgann
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc gatgagcttc gaccctgaaa
    12841 tgatcacggc tgctattcgc aaacacctgc cagattttaa gttaacttac aatgtcgatc
    12901 ccatcaaaca ggcgattgcc aacagctggc caaattccat ggatgacagc agcgccaggg
    12961 ccgaatgggg ctggaaacat gactatgacc tggacgccat gacagccgat atgctggata
    13021 aacttcgcgt ccgtctggct atctgaggtt gagatgctga aaatcgcggt agtgggagtc
    13081 ggtcaccttg gcaagcatca tgcccgcaag tttgccgcta tgcctgatgt gttactggcc
    13141 gggatttacg accaggatac ccaacggtgc caagaattgg cccaggagct tgaggtaaaa
    13201 gcgtttggaa actatcaggc gctgctggac gcgtgtgacg gagtggatat ctctgccacc
    13261 accacagctc attacgatct ggccaaaacc gccctgctgg ctggaaagca cgtctttgtg
    13321 gagaagccca tcactgccga gctcggtcag gcccgtgagc tcgtcagcct ggccgcccag
    13381 caaaatctca agctgcaagt gggccatatt gaacgtttta accctgtcat cctggctgtg
    13441 gaagatgaga ttgccaatcc tatgttcatt gaatcgcacc gcctttccac atttcatcac
    13501 cggggtaccg acgtcccggt ggtgctggac ctcatgattc atgacatcga cctgatccta
    13561 gattttgtaa aaagccccgt gaaagacatc cgcgccagcg gagtgggcat cctcactccc
    13621 agcatcgaca tcgccaacgc ccgcctggag tttgaaaacg gtgctattgc caatgttacc
    13681 tcttcccggg tgagcatgaa acaggaacgc aaaatccgct ttttccagcg tgatgcatat
    13741 ttctctctgg attttggcgc taaacacgcc accgtgatga agcgatctcc agatatctta
    13801 aaatacctgc ctcaaatcct ggctggcagc accgacatca aggccgagca gctagtggac
    13861 gtcaagcatc acgacctctc tctggaagct aaagatgctt taaacatgga gcttgagagc
    13921 tttgtgactt gcattgtcaa ggataagccc cccatcgtgg atggcgtagc cggcaccaga
    13981 gctttggaag tggcgctgcg cattctggag atcatcggcc accaacccaa aacacacatt
    14041 taaggttatc tatatgagct atattcagat aaaagacata ccccagcatc ttgagcgcga
    14101 gatcagcatg cgtggctggg tgcggaacat ccgtcacagc ggcaagctgc tcttcatcat
    14161 tctacgcgac ggcagcggtg aaatccaatg tgtggccttc aagccagaac tgggcgatga
    14221 aaagtttgag ctggcccgta aacttacgat ggaatcttcg gtgatcatca gcggaatcgc
    14281 caggccccat caaaaaatgc aagaccaata tgagcttgcg gttactgatt tgatgccagt
    14341 ccagatcgcc gatgaatatc ccatcagcaa aaaagaacac ggaccagatt tcctgctctc
    14401 caaccgtcac ctctggctga gagctcccaa gcagtgggcc ttgatgcgcc tacgtcacac
    14461 cgtctatttc gccatctgcg attacctcaa tcaaaacgac ttcttccgct ttgactctcc
    14521 cattctgact cccaacgcct gcgaaggcac caccactctc tttgagctgg actacttcga
    14581 tgagggcaaa gcatatctct cccagagcgg tcaactctat ctggaaacag gaattatgag
    14641 cctgggtaga gtgtatgact ttggcccggt gttcagggcc gaacgctcca agactcgcaa
    14701 gcacctcacc gagttttgga tgatggacgc tgaggccgcc tgggtggaac acgaagagaa
    14761 catggccatt caggaagggc tgatccgctt tgtaatccgc accgtactgg aacgctgcga
    14821 ccgggaactc ggcatcctcg aacgtgacaa agaagctctc aaagccgccg acgctccgtt
    14881 cagaagaatt acccactacg aggctatcga gtatctgcgc tccaaaggca gcgagatcac
    14941 ccacggcagt gattttggag cagcggaaga ggccctgctg acggaaggat caccggtccc
    15001 cgtctttgtg gaaaaatggc ccaaagagat caaggccttc tatatgaagc gcgatcctga
    15061 aaaccccaac ctggtgctgg gcagtgactt gatcgctccg gaaggctttg gagagatcat
    15121 cggcggcagt cagcgtgaag atgatcacga gctcctgctg gggcgcatga aagatgaaaa
    15181 gatgcccatc gatgattatc agtggttcct ggacctccgc aagtatggca gcgtccccca
    15241 cagcggtttt ggcgtgggcc tggaacgcct cgtgacctgg atgagcggca tccaccatat
    15301 ccgggagact atccccttcc cgcgcatgat ttatcgcatc tacccctgac atcacaggtt
    15361 gagaaggttg aatagtccaa cgggtttccc gcagattacg cagatcgatg cgcaaatatc
    15421 gcagatgttt agaatgttta aagggtttag gcccctgtct tctcaacctt ctcaaaattc
    15481 gcgagatctg cgcattaatc cgtgggaaac aagcctgctt tcattccagc gtgcttacgc
    15541 tagatctatt ataaataaaa catatagagg ttagcatgaa ttatgccagc gacttttcgc
    15601 gggtcacaca gacgatgaaa tcctccctga tcagagagct tgtggccgcc acaaaaagca
    15661 tcccgggatt gatttccttc gcgggtgggt ttccgtctcc ggccaccttc cccaaacaga
    15721 tcctcgcgga actgtatcgg gaagttatca cccaggacgg actggatgtt ttgcagtacg
    15781 gtgccagcga tggagatgcg gtgctcaagc gggaactggt caaatgggaa ggctatgaca
    15841 ttgccgccaa tcagatgatg accaccgttg gagctactaa cgccatatac tactatgcct
    15901 taagtttgtt ggacccgggt gatgtggtga tctgcgaagc tcccactttc ttgggcacgc
    15961 tggtagccct ggatgcgctg caggctaagc tggtaagcgt ccccatcgat aacgacggga
    16021 tcagtctgga acacctggaa gccgctgtca gaagctgtaa agcggctggc aaaaagatca
    16081 aattcatcta cacgatcccg gattttcaca atcctggcgg agtgacgatg agcatgcagc
    16141 gccgtcgtga cctgctcaac ttcgcgattg aacaccagct tcccattctt gaggacaatc
    16201 cctattccag attgcgcttt gacggaaaac ccctccccac tctatacagg cttatgcgcc
    16261 aggagtttac aggcagcgag atcgtgaccg aagtggtgtc gttttccaag attctgggcc
    16321 ccggtatgcg catcgctttc gtgaaaggcg aggccagtct gctccagaaa atggaatcct
    16381 ggcagcagaa agtgaatgtg actccagact gtgtatccca gcgggtggca gccagatttt
    16441 tggccgatgg ctttatggag ccacatttgc aaagcgtctg tgagcattac aaaccctact
    16501 tgcgaaaaat gctcgattgt ctggaagcct atatgccaaa aggggttagt tggactcgac
    16561 cccagggcgg aatctttgtg tggatggagc ttccggagca ccagaacgcc gatgaaatct
    16621 tccacgccgc cgctcagaac aaggtgtcct ttatcccggg cagcaaattc tatcccgctg
    16681 gccaggaacg cttcaacacc ctcaggctca acttcaccta ttcgtccctc gatcagatcg
    16741 ataccggaat tcaacgtctg gcggagctgc tcaaggcttt agcttgacaa gaatgccgat
    16801 cggcttatct tgtccttagt atctgattcc tgaacggata tgtgtaacag cgggtctttt
    16861 agcccgcgct tgaaaaggag ttacagatga acaagctcgc aattacgcta atcgcgctgg
    16921 cagttgcttg cctgcccttg gccgcgcaaa tagatctgcc caagcccgat ttcagtctga
    16981 tgccccgagg cgggagaagc tttctggaca ccaacaagct ctcaatgtca cattccatgg
    17041 gctttgaggc cggttcttcc tcttatggcg gatactatct gtcccgctac accaatcatc
    17101 tcaaatacag cttcaatccc aagctggacc tgcaagtgga cctcagcctg atcaattttg
    17161 gcagtgccag tcctgctttc aagatcaatg acgacaacag cagccgcttt gttcctggct
    17221 tcaccctgga ttatcatccc tccgagagct ttaagctcca ggtgaaatac cagcagggct
    17281 accccatgtg gggaaccatc aagccttggt acgagctgga acgtccctga ttgggagtac
    17341 atgatttgac agcggttatg atcatcttgc ttgctgccct gggagcttcc ctgggcagtt
    17401 tttttaacgt cctcatccat cgtcttcctc agggaaaatc catcatcaaa cccgcgtcca
    17461 gctgtgcggg ttgcggatac cgcatacctt tctacctcaa tattcccatt ctcagctaca
    17521 ttatcctgaa gggcagatgc aagcagtg
//
LOCUS       DAOSFN010000040        17417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2657_length_17417_cov_10.032830, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000040 DAOSFN010000000
VERSION     DAOSFN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 17417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17417
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2657_length_17417_cov_10.032830"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..2403)
                     /locus_tag="PLO35_04115"
     CDS             complement(343..2403)
                     /locus_tag="PLO35_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOH78882.1"
                     /translation="MYSRFRLPILLVLLSAITLLIAADEVPIPVSSQNRLNDQDILAE
                     FDGGTITRKDVDSRISKIPPNQQGRFRTVAGQTQVLDVMALEEVLMAKALQLGIDQNP
                     DVLSKIDAGLRQFYIQEYYTRNVTEKVQLTDADKRAYFNDNPQIFIVLPLVTINYIQA
                     ADEASALKALDELKSGAGWEQVSEAYNQNTYVKGLKGVIKNIRLNGNIPGVGNDTDLE
                     ALIQANTANLNALIGPVQTNTGWHIFTVVDFKEGRSKTYEEVIPEIEQRLRPVTEKNL
                     LDQIKSELMLKYQVSIDSAMIAKVDLNSQAKNDPIKDQPVLSSNHPELSFTVATLLEA
                     FNRLAPQEQVFYVKGEGAVQLIDQELIRALLYLDAKDNGYEQFFEQKDEYQQMRRFYI
                     LNTAYRQLVIDSVVVPQEEVRAYYDSHIQDYTTPASRAIQILWFKDQNSADRTWKKFQ
                     RMYKLGDEKRMQQLIDEESTNPALALLDNQYNNGVVTGIGPDAEFSKRIWDNPVGYLS
                     PVFTSARGDLVFFRTLRENPPVVKSFTEIEPRIFGILKKEKEAGQQDKINKELSEEFN
                     LVKYPERINLLLSAEELFTLADDAARMRNFKDAITFYDQIIQNYPNGNDDYKAYFMKA
                     FLVAEELKNNDLALDLFKSFLAKFPTGDLNESAQFMIDTLEGKLVLEIEEEPAIPEAS
                     QEME"
     gene            2578..3015
                     /locus_tag="PLO35_04120"
     CDS             2578..3015
                     /locus_tag="PLO35_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOH78883.1"
                     /translation="MLSKLKNQKGFTLIEILVVVIIVAILAALAVPRYLRYVEKSRST
                     EAQTAISTLRKVFDVKLQTDGTTEGFTKEQALKEARLGESTIKNWQFDITGTPPTKYI
                     ATSTADFPGGEGKQVWYDVKEAKFHGYGIDTWTNPESGGTETD"
     gene            3031..4086
                     /locus_tag="PLO35_04125"
     CDS             3031..4086
                     /locus_tag="PLO35_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOH78884.1"
                     /translation="MTIHELLRFTAEAGASDLHVASGSHPMVRVNGKMKRLNLPIQTL
                     EDIESLVYGVMNESQQELFRKHLEIDFSTKLSNDVRFRVNAFHQINGISAAFRVIPNE
                     IKHYEELHLPEVMKRLTTKEKGLILVTGPTGSGKSTTLATMIDTINENRFCHIITVED
                     PIEFVHRSKNCLINQRELGHDTWSFTAALRSALREDPDVILVGEMRDLETVSLALTAA
                     ETGHLVFATLHTGSCTKSIDRIIDMFPKEQQQQVRSMLSESLEAVISQTLMPTKDGKG
                     RVPAVEIMIANAAVRNLIREEKTYQIPSVIQSSTKEGMQTRDQSLYNLVMNNLVERTL
                     AEELADNPKLFATGAGF"
     gene            4208..4624
                     /locus_tag="PLO35_04130"
     CDS             4208..4624
                     /locus_tag="PLO35_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78885.1"
                     /translation="MNILRQTRGFTMIEALVLVVVIVILIVSLYIGIVYAEKTLATNY
                     RDRVVTLLLAGELEMEYYRHTRGYDFQLQNSQEYVIQEFDRGRPLTGRLTISSLSGME
                     TSNQRTLNYVALTATMTWIDPVSKRPRVMRMREDYF"
     gene            4624..5280
                     /locus_tag="PLO35_04135"
     CDS             4624..5280
                     /locus_tag="PLO35_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78886.1"
                     /translation="MKRLFSKAQIPLANQRGVSLVEVIAVMLIIVIMIVISAGGIALF
                     FRKYNELKAYAELQADGLEVINYIKNGIPVGSQEIRRANNGEMQFIRPKEYFGVNNAI
                     SIRFLQAPYGASQANGIRVVPIETSSIIAPTDFADFYLYDGTIRCNWRYRGIAQAAPV
                     TLFPKTGKRDYMFLDQITFRRINTDAEIWALEVSLKARVKVGPKQYKSIHYRTKMARK
                     "
     gene            5313..7205
                     /locus_tag="PLO35_04140"
     CDS             5313..7205
                     /locus_tag="PLO35_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78887.1"
                     /translation="MNEILTKPNGNISIAMLLMVISMMSGFTMASMALRDVISFQYDY
                     ESIQSQIMLRSEAYRGQAIMEKIGGVIVPLSTSERTVEISGTNMKKTFKIKSLLTPGG
                     FEMSSDDVVAGDQKQVTQVHSRVNTKAGIGQAAYTNPRRLIRKYGVFTLETETFAKFM
                     YFTDIESDPNGFPVRFYGPDVLYGRVHSNQDIWIRQAGGGNNNNWPTFWGWVSTCGVI
                     KVYPDGGHTFPETDVFRGGLSEGYPYTVFPEQATRIRRNGTVLGPSYFDVNKIVYADV
                     DGVSAQAMLGTIQDPIRQFADVYTNNSFPPGQGEPAWTNQYTVRDTMWVNLGSSSTIN
                     KSRFVYGRLWLKGNFSRYQTWGCGDTLFIIGDILLQGTPRGDSPSPNRRDVVGLVSEK
                     SILVKYGYRSPIDSLRYKETSGPTTGNPLGGVWIYAAMAALGDGHGNARKDGVFTFEY
                     QHPHPSTPDYRIGNTTFTKIDLHRRRYPQTSSAAWPPLLDYPWYNPLWPERFPYCERG
                     YINVHGSISQRRRGFVHRNYTDADYPNNAGIWNQPIDFCGGTSSPNGTLQTDPVLGFQ
                     MATQHAPGATGTGVGYQKNYHYDDRFYKTSPIDFPEINRKDETPFAAVKWEIRSPRLI
                     PSQIIN"
     gene            7226..8296
                     /gene="pilM"
                     /locus_tag="PLO35_04145"
     CDS             7226..8296
                     /gene="pilM"
                     /locus_tag="PLO35_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOH78888.1"
                     /translation="MKKQKTLKFKESVGIDLGSHSVKVVHLKRLHEGFKLLNYEIRPT
                     VAQGVEYIPSDLRPERYGPVLVEILKTLRINPKKVQHLVTSVGGDNTSIKQIKTIFLP
                     DEELESALFFEAKKHIPISGSDMVLDYQVLSVEEKTNNMNILLGATSKELLNEHTNIL
                     QTAGLSPNLVDIDSLAVANSFALNAYVEDGVYVLLNLGAHRTNMVIWGPEAKFFSRDI
                     PYGGYHFTRDIMRKRQLEWNDAENYKLEWGLMEDPSNPEVRTLSMLDITEKPTEDAIV
                     EEIRRSLRFYVKEAGNSDFRKVYIMGGTSKLRGLLEYIQEKVSIPTEVFMPFINVEMP
                     EKFMDKKDPQLALAIGLAMRAE"
     gene            8306..8908
                     /locus_tag="PLO35_04150"
     CDS             8306..8908
                     /locus_tag="PLO35_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78889.1"
                     /translation="MNNAFYFKINLNKYGDLKIQTEREKKNFYQAIAVFVLGTLIILG
                     VHFVLSSRLGAKVDSRRKFLKETERQLESFQTSDDYLSSQDLDRLEQTFNNRIFWANK
                     LVALSQEIDNKLAVRKFTFANGVLTLNGITPVDNNVRELDLINEFILRLKSNPEISND
                     FPQIKSGLITKQMVKDTAIMEFVIECYSKEVPSTPGGEQR"
     gene            8905..9459
                     /gene="pilO"
                     /locus_tag="PLO35_04155"
     CDS             8905..9459
                     /gene="pilO"
                     /locus_tag="PLO35_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOH78890.1"
                     /translation="MREKYIVFILLMVLIAVLIFTASANNLQSNLKKVREYDEQIKVA
                     QEKLNSARIMDEQLSQFSLIIDNSLSRSGGFTFDEINEFKKRIGELAHERKISINKLS
                     DSNKFSLPGLLETTYNLEMEATFVQMGQFLSDLEALDNIIKIHAIDVTPAQVEDGNKK
                     TVDTAVSKYRIMLELSIFKVKKEA"
     gene            9460..10002
                     /locus_tag="PLO35_04160"
     CDS             9460..10002
                     /locus_tag="PLO35_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78891.1"
                     /translation="MQLRYVKDFAITLIVIIFLVLGIRLYTNYFKAKDVPQQSIHSKE
                     SVSDTLLSKIKGIEKSIQDRKTFVFSINRDPLRQGNIIKDRLDLEREFVDNLRNTFRL
                     SSTSITENGNKIATIEYRDQNYYATIGDVVEGRRITEIGTDWIRYYFNGSTYTTQVAP
                     RPPMPDFSTINPSELQSGNF"
     gene            10035..11438
                     /locus_tag="PLO35_04165"
     CDS             10035..11438
                     /locus_tag="PLO35_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOH78892.1"
                     /translation="MRLTPSSKPFLRIILLLAMAWLAVSMMAQSNKLTTKVSYNADDA
                     TLSSVMNALARQSGTNIVLAVDPGQAGDKKEERRVTINIKDVPIETAVSLVARASGLA
                     YRVLGENTFLVGNKQNIMEESGERSNIIYLKNLDAQKVARSLENTNGVIVPLEGQNAI
                     MVYANPETFKNIQDLVTKIDLEQKQIEIRVRLIEIHLSNAKKMGIDWSRLNHLTTILA
                     EDPTNARGSGLPYNYTDVDGYLPHGDPTDFEQLPDEQYFQRINGLKDIGHFSRQLTAF
                     DITIDWLLENNAAKLLTDTRVTALNGTEATIHIGEVVPFVVTDNDKQIQVEREEVGII
                     LKVMPSVNQDGLITATIAPEVSSVTDLVGGYVPRTKVRRVTTSVTVPNEQKIIVGGLL
                     NSNISQKTSKLPFLGDLPFIGRLFQHRYEVVENTDLIMEITPRLVSITEPSPEPKVDE
                     RLTRALIEYEDNQNSQK"
     gene            11449..13722
                     /locus_tag="PLO35_04170"
     CDS             11449..13722
                     /locus_tag="PLO35_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78893.1"
                     /translation="MKTKRIPIITIMALLLVMLATSCDRRNIPVVIDQEPVPISSERY
                     IDDISATPDIIYADYGITYSLIKVLVEDGEGFGVPSQIVSFKTTLGRILTNVPTDSTG
                     VATTTLWDAGVAGEAQVTAVVRKYSDEDPTVVLSEATQTVTVNILPVPDIDSVTLEFQ
                     STLNPFPMTVMQTTEVSAAVKNVLDEYVPNNTLVTFTCSKGRFVDAAGNVLGVSVVAK
                     TTNGRATVNYNSGATSTTSPGMENALIRAAIGTVYSEREVVIRAGAPAVIDLRSYVFA
                     DGDSTETQTNPVDSANEIKLYATLNDLHGNACQTKPVKFSTDLGTFMNTTQLITVNTG
                     ADGKAITRLIPGLQAGAATIQASANGDTLITQLIFSVTSSEIHSISFTQDTQIDLSVA
                     NTGGTESAILRVKLRDINGNLIDTQKQVWFRLLWNNPNDPHPEGANLNNQPPNDSVMV
                     MSTGGEAQISVNSGPESGVLVVRASHIRESDSSWIRATKANILIHAGPPATITPFIGR
                     FNTGQNMGGGLWRVIAGAEVYDIHGNPVDKNTSVFFEIINNTTSCTIEGNGYVGNVSV
                     EDDSMAAIAYTIVTYSGVHTNEMITIQATTGSGTGAIVQGFATLPLPLNDPRFEIQAN
                     PQSLNFGDTSPDWKSTEIYCVLTDGQGCLIGNQEIMLVSTKGQFVAIPGFNNDYPAAP
                     VWMIKTDDGSYGTGNYAGWAWGQIRFHRLECPAGDPQAQSPGQTTGSIIGRILGTAVT
                     DQTDVAIYRYWTPAPPF"
     gene            13882..15591
                     /locus_tag="PLO35_04175"
     CDS             13882..15591
                     /locus_tag="PLO35_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOH78894.1"
                     /translation="MTYNPQFARIGEVLVHEGFVNEDQVKEAMIKQTNFGLKLGETLI
                     KLGYLREKDLLSALQMQLGYDIVEDRELMDLDLEIVSLIPEPYALENRVIALREEGDG
                     IVVGIADPENLTVLDSLKKLLGKNVKPELIGDSALHDTIEKYYKSIRTTSQVEDAVGG
                     FDFVAVDEDENEITIANASTSADAPVVKLVNLIINEAIKAGATDIHIEPLIKTSRIRY
                     RIDGALREVMTPPIGMHAGVVSLTKVMSKLNIAERRLPQDGHIALKTSMKSVDVRVSI
                     TPTVLGEKVVMRLLDKGDFGFKLGTLGFEEEDMAIFAKIIRRPYGIIIVSGPTGSGKS
                     TSLHAALKEIQNIESNIITVEDPVEYRLEGITQIETKEQIGLTFGSALRSVLRQDPDI
                     VLIGEIRDEETADIAIKFSLTGHLVFSTLHANDAPSTVTRLIDIGIKPYLVGSSLSLV
                     MAQRLIRKICPYCIDDYAPTAQEISDCGLTPEEASQIKFKMGKGCVHCDNTGFSGRTG
                     IFELLTVDAEIRRLVYEGGNQDLIRDAALRSGMRTLHDAAISKMKRGLTTIREVIKMT
                     VIE"
     gene            15617..16870
                     /locus_tag="PLO35_04180"
     CDS             15617..16870
                     /locus_tag="PLO35_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOH78895.1"
                     /translation="MPNFAYIVKDAKGARVEGILKADTLDMAVDKLAKEGSTIISVKA
                     AAEGAFKGKLSLFDKLMLQVYKWRTGVSLRTLVFFTRQLSTMFSAGLTIEKAITDLEK
                     EEKNKRFGRVLRKLSDDIRKGFSLSEAMEQHPGVFNPLYVALVKAGEVSGTLHTVLDE
                     LSDYLEKIEDTRRKVMSSMAYPIFILVFLGFVTWALFYYIIPLFAGVYGDFGADLPAP
                     TVMAIKVSNFIVANVFATILIVIAAVIGLFLVYLTDRGRYAMDAFKLKIPVIGRVLSN
                     SIMSKFSRTFSILMAAGVPIMDTMELTENVVQNAVVEGGVRKARVMVKEGYGVANAFR
                     KTGLFPPTLLQMIATGEETGDMDKLLGKAAQFYEKLVDSVIDRLTSLIEPLLIVIMAA
                     IVGGIIVIIYLPIFDLGDAISQGFN"
     gene            16986..17234
                     /locus_tag="PLO35_04185"
     CDS             16986..17234
                     /locus_tag="PLO35_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78896.1"
                     /translation="MIDQTQATGSELLSLVRKIMHDVNTPLCVINLSLNRLETVMDAQ
                     PHPELSKSYEEISASADKLLEIMSRLDQARAMIEESGI"
BASE COUNT         4280 a         4974 c         4377 g         3786 t
ORIGIN      
        1 gggacggcag tttagattag accagtacga tgataatgag ttataaatat atgcatttat
       61 ctatctaaac tgtcgtccct acaggactca tctttgtttg ggacaccgga tctatctaaa
      121 ctgtcgtccc gccgggactc gtaaggaatg atgccacgat tatcagtttc cgcacaagct
      181 cagaagccag tggcatgacc ttcagcaagg gtcttttatt ggcgcggcgc tttgaaatcc
      241 aggggggtga tcgtttacca cagccgcaag ctacgcatca actcccccac aaagaataag
      301 cctccgaatc ggaggcttat tatatcatag ggctgaggta tcttattcca tctcttggct
      361 ggcttcgggg atggcaggtt cctcttcgat ttccagcacc agcttcccct ccagggtatc
      421 gatcatgaac tgggcggatt cgttcagatc gccggttggg aatttggcaa ggaaggattt
      481 gaagagatcg agggccagat cgttgttttt gagctcttcg gctaccagga aagccttcat
      541 gaagtaggct ttgtagtcgt cgttgccgtt gggataattt tggatgatct ggtcgtagaa
      601 agtgatggcg tctttaaagt tgcgcattct ggcagcgtcg tcggccaggg tgaaaagttc
      661 ttcagcgctg agcagcaggt tgatccgttc cgggtatttg accaggttga attcctcgga
      721 aagctctttg ttgatcttat cctgctgtcc ggcttccttt tcttttttca gaatgccaaa
      781 gatgcggggt tcaatctcgg taaagctttt taccacgggt ggattttcac gcagggtgcg
      841 gaaaaacacc agatcgccac gagcggaggt gaagaccggg ctcagataac ccaccggatt
      901 gtcccagatg cgtttggaaa actcagcgtc aggaccgatg ccggtaacca caccgttgtt
      961 gtattggttg tccaatagcg ccagggcggg attggtggat tcttcatcga tcagttgctg
     1021 catgcgtttt tcgtctccga gcttgtacat gcgctggaac tttttccagg tgcgatccgc
     1081 ggaattctga tctttgaacc agaggatctg gatagccctg gaagcaggag tggtgtaatc
     1141 ctggatgtgg ctgtcataat aggcccgcac ttcttcctgg ggtaccacca cggaatcgat
     1201 caccagctgc cggtaggctg tgttgaggat atagaagcgc cgcatctgct gatattcgtc
     1261 tttctgttca aaaaactgct cgtagccgtt gtctttggca tccaggtaga gcagcgcgcg
     1321 gatgagttcc tgatctatga gctggaccgc gccctcgcct ttgacataaa agacctgttc
     1381 ctgaggagcg agacggttga aagcttccag cagggtggcc acggtgaagc tgagctccgg
     1441 gtgattggaa ctaaggacgg gctggtcttt gatggggtcg tttttggctt gggaattgag
     1501 gtccactttg gcgatcatag cgctgtctat gctcacctga tacttgagca tcagttcgct
     1561 tttgatctgg tccaaaagat tcttttcggt cacgggacgc agacgttgct caatctctgg
     1621 gatgacttcc tcataggtct tggagcggcc ttctttgaaa tccaccaccg tgaagatatg
     1681 ccagcctgta ttggtctgga cgggtccgat taaagcgttg agattggctg tgttggcctg
     1741 gatgagggct tccagatcgg tgtcgtttcc cactccgggg atgttgccgt tgaggcggat
     1801 gtttttgatc acgcctttga ggcctttcac gtaggtattc tgattgtagg cttcggaaac
     1861 ctgttcccag cccgccccgc tcttgagctc atcaagtgcc ttcagcgcgc tggcttcgtc
     1921 ggccgcctgg atataattga tggtcaccag gggcaaaacg ataaagatct gggggttgtc
     1981 gttgaagtag gcacgtttgt ccgcgtctgt caactgaacc ttttcggtga cgttgcgggt
     2041 gtaatattcc tggatgtaaa actgacgcag cccggcatct atcttgctga ggacgtcggg
     2101 attctggtct atgcccagct gcagcgcttt ggccatcaaa acttcttcga gggccatgac
     2161 atccagcacc tgggtctggc cggccacagt gcgaaaacgg ccttgctgat tgggcgggat
     2221 tttggaaatg cggctgtcca catccttgcg ggtgatggtt ccaccatcga attcggcaag
     2281 gatatcctga tcattcaggc ggttttgact gctgaccgga atgggcactt catcggctgc
     2341 tatcagcaag gttatcgctg agagcaggac cagcaggatc gggagtctga agcgtgaata
     2401 catattttac tccttaattg cgaggaactt tttgtgtgtg acccaaaaaa agattgacca
     2461 gataggcgtc aagtcattta ttggcttaaa agattccgcc acaatttatc ggcggtttgg
     2521 cacgtaatgt gcatagtatc gatttacgaa aaaacacttc ccaggaggaa aaccaaaatg
     2581 ctgagtaaac tcaaaaacca gaaagggttt acccttatcg aaatattggt ggtggtgatc
     2641 atcgtggcca tcctggccgc cctcgccgta ccccgttatc tgagatatgt ggaaaaatca
     2701 cgttccaccg aagctcagac cgccatcagc accctgcgca aagtattcga cgttaaactt
     2761 cagacagacg gaaccaccga aggattcacc aaagaacaag ctctcaaaga agcccgtctg
     2821 ggtgaaagca ccatcaaaaa ctggcagttc gatatcaccg gtaccccccc taccaaatac
     2881 atcgccactt ccaccgcgga tttccctggc ggcgaaggca aacaggtttg gtatgacgtc
     2941 aaagaagcca aattccatgg ctatggcatc gatacctgga ccaacccgga atccggaggg
     3001 accgaaaccg actgatctgg aggaactaag ttgactattc acgaactact acggttcaca
     3061 gccgaagccg gagcctcaga cctgcacgtg gcatccggct cgcatcccat ggtgcgtgta
     3121 aacggcaaga tgaaacgtct caatctgccg atccaaaccc tggaagatat cgaaagtctc
     3181 gtctatggcg tgatgaacga aagtcagcag gagctcttcc gcaagcatct cgagatcgac
     3241 ttttccacca agctgtccaa cgatgtgcgt ttccgtgtga acgcgtttca tcagatcaat
     3301 ggtatttccg ccgcctttcg tgtgatcccc aatgagatca aacactacga agagctgcat
     3361 ctgcccgaag tcatgaaacg cctcaccacc aaggaaaagg gcttgatcct ggtaacaggt
     3421 cccaccggta gcggcaaatc cactaccctg gccaccatga tcgatacgat caacgaaaac
     3481 cgcttctgcc acatcatcac cgtggaagac cccatcgagt ttgtgcaccg cagcaagaac
     3541 tgcctgatca accaacgcga actgggccac gacacctgga gcttcacagc ggcgctgcgt
     3601 agcgctctgc gtgaggaccc ggacgtgatt ctggtgggtg aaatgcgtga cctggagacc
     3661 gtttctctgg cccttacggc cgcggaaaca ggtcacctgg tgtttgccac cctgcatacc
     3721 ggaagctgca ccaaatccat cgaccgtatc atcgatatgt ttcccaagga acaacaacag
     3781 caggtccgct ccatgctttc cgaatctttg gaagcggtga tctctcagac cctgatgccc
     3841 accaaggacg gcaaaggccg cgttcccgcc gtggaaatca tgatcgccaa cgccgcggtg
     3901 cgcaacctga tccgtgagga aaagacctat cagatcccct ccgtgatcca gtccagcacc
     3961 aaagaaggaa tgcagacccg cgaccaatcg ctgtacaacc ttgtgatgaa caatctggta
     4021 gaacgcacgc tggccgagga gctggcagac aaccccaaac tctttgccac aggagctgga
     4081 ttctaagatg tctttgatca tgggtagcaa acaggtgccg gaaagcgcaa gctctttggc
     4141 cccctacgga gaaaaaccga ggtcacagag gcaaacggtg acgtttgcga ctccacaagc
     4201 gggacccatg aacatacttc gccaaaccag gggcttcact atgattgaag ccctggtttt
     4261 ggtggtggtg atcgtgatcc tgatcgtaag cctgtacatc ggcatcgtgt atgccgaaaa
     4321 gaccctggcg accaactatc gcgaccgggt ggtgaccctg ctgctggctg gcgaacttga
     4381 gatggaatat taccgccaca ccagaggtta cgactttcag ctgcaaaaca gccaggagta
     4441 tgtgatccag gaatttgacc ggggccgccc tctcactggc agactgacca tctcctccct
     4501 atccggaatg gagacctcca atcagagaac gctcaactat gtggccctca cagccacgat
     4561 gacctggatc gaccccgtct ccaaacgacc ccgggtgatg agaatgcggg aggattattt
     4621 ctgatgaagc gcctgttttc caaggcccaa atcccccttg ccaatcagag gggcgtttcg
     4681 ctggtagagg tgatcgccgt gatgctgatc attgtcatca tgatcgtgat ctcagcggga
     4741 ggaatcgccc tcttttttag aaaatacaac gagctaaaag cttatgccga actgcaggca
     4801 gatggcctgg aagtgatcaa ttacatcaaa aacggcatcc cggtgggctc acaggaaatc
     4861 cgccgggcca acaacggtga gatgcagttt atccggccca aggaatactt cggggtcaac
     4921 aacgccatca gcatcagatt tttacaggct ccctatggag cttctcaggc aaacgggatc
     4981 agagtggtcc cgattgagac ctccagcatc attgccccca ccgattttgc cgatttctat
     5041 ctttacgacg gcactatccg ttgcaactgg cgctaccggg gcatcgctca ggcggcccca
     5101 gtgaccctct tccccaagac aggcaagagg gattacatgt ttttggacca gatcaccttc
     5161 aggagaatca acaccgatgc cgaaatctgg gcactggaag tgagcctgaa agccagggtg
     5221 aaagtgggtc ccaagcaata caagagcatt cactaccgca ccaagatggc acgaaaataa
     5281 tcgtccacca agcttctaac cgggagtaac ttatgaatga gatattaaca aagcccaacg
     5341 gcaacatcag catcgccatg ctcttgatgg tgatctcgat gatgtccggc ttcacaatgg
     5401 ccagcatggc tttgagggat gttatctctt ttcagtacga ttatgaaagc atccaatctc
     5461 agatcatgct gagaagcgaa gcctaccgtg gccaggctat catggagaag attggtgggg
     5521 tgatcgttcc gctctccacc agcgaacgca cggtggaaat ctccggaacg aatatgaaga
     5581 agaccttcaa gatcaagtcc ctgctcacac ccggtggatt tgagatgtcc agcgatgacg
     5641 tggtggccgg agaccagaaa caggtcactc aggtccacag ccgcgtaaac accaaagctg
     5701 gaatcggtca ggcagcctac accaatcccc gcaggctgat ccgcaagtat ggagtgttca
     5761 cgctcgagac cgagaccttt gccaagttta tgtactttac cgatatcgaa tcagacccca
     5821 acggttttcc agtgcgcttt tatggcccgg acgtgctcta tggtcgcgtg cacagcaacc
     5881 aggatatctg gatcagacag gccgggggcg gcaacaacaa taactggccc accttctggg
     5941 gctgggtatc cacttgcgga gtgatcaaag tctatccgga cggcggtcac actttccccg
     6001 agaccgacgt cttcagaggc ggtctctccg agggttatcc ctatacggtc ttcccggaac
     6061 aagccacccg gattcgcaga aacggcacgg ttttggggcc cagctacttt gacgtcaaca
     6121 agatcgtcta tgccgatgtg gatggcgtga gcgcccaggc catgctggga accattcagg
     6181 accccatccg gcagtttgcc gatgtttaca ccaacaacag ctttcctccg ggacagggtg
     6241 aacctgcctg gaccaatcaa tacacggtgc gagacaccat gtgggtaaat ctgggctcct
     6301 cgtccacgat caacaaatcc cgctttgtgt atggtcgctt gtggctgaaa ggcaacttct
     6361 ccagatatca gacctggggc tgcggtgata ctctctttat tataggcgac attttattgc
     6421 agggcactcc ccgcggcgac agtccatccc ccaaccgcag agacgtggtg gggctggtgt
     6481 ctgaaaagtc tattctggtc aaatacggtt accgcagtcc gatcgactct ctgcgttaca
     6541 aggaaaccag cggtcccacc accgggaatc cccttggcgg agtctggatc tatgccgcta
     6601 tggccgccct gggtgacgga catggaaacg cccgcaagga cggtgtgttt acctttgaat
     6661 atcagcaccc ccatccctcc acaccggatt acaggatcgg caacaccacc tttacaaaga
     6721 ttgatttgca tcgcaggcgt tatccgcaaa cctcttcggc agcctggcct ccgctgcttg
     6781 attatccctg gtacaatccg ctctggccgg agcgctttcc ctattgcgag cgtggataca
     6841 tcaacgtcca cggctccatt tctcagcgcc gcagaggctt tgtccaccgc aactataccg
     6901 acgctgatta ccccaacaac gctggtatct ggaatcagcc cattgacttc tgcggtggca
     6961 ccagttcacc caacggcacc ctccagaccg atcccgtcct gggcttccag atggctaccc
     7021 aacacgctcc cggagctact ggcaccgggg taggctatca gaagaattat cattacgacg
     7081 acaggtttta caagacgtct cccattgact tcccagagat caaccgcaag gatgaaaccc
     7141 ccttcgcggc tgtgaaatgg gagatccgga gcccgagact cataccttct cagattataa
     7201 attaacgtca tagataagag gaactatgaa gaaacaaaaa acgcttaagt tcaaggagtc
     7261 ggtcgggatc gatctcggca gccacagcgt caaggtagtc cacctcaaga gactgcacga
     7321 aggctttaag ctgctcaatt atgagatccg gcccaccgtc gctcagggag tggagtatat
     7381 tcccagcgat ctgcgtcctg aacgttatgg tcccgtcctc gtggagatcc ttaagacgct
     7441 gcgcatcaac cccaaaaagg tgcagcatct ggtcacctcc gtcggcggcg acaacaccag
     7501 tatcaagcag atcaagacca tcttccttcc tgacgaggag ctggaatccg cccttttctt
     7561 cgaagccaaa aagcatatcc ccatttccgg atcggacatg gtgctggatt accaggtgct
     7621 gagtgtggaa gagaagacca acaacatgaa catcctgctg ggagcgactt ccaaggaact
     7681 gctcaatgaa cacaccaata tcctccagac tgccggactc tcacccaacc tggtggacat
     7741 cgattcattg gcggttgcca acagctttgc cctcaacgcc tatgtggaag acggcgtgta
     7801 tgtgttgctc aatctggggg cccatcgcac caatatggtg atctggggtc ccgaagccaa
     7861 attcttttcc cgcgacatac cctatggcgg atatcacttt acccgtgata tcatgcgcaa
     7921 gcgccagctg gaatggaatg acgccgagaa ctacaagctc gaatgggggc ttatggaaga
     7981 tcccagcaac cccgaggtcc gcaccctcag catgcttgac atcactgaaa agcccaccga
     8041 ggatgccatt gtggaagaaa tccgccgctc tttgcgcttc tatgtgaaag aggccggcaa
     8101 cagtgatttt cgcaaggtat acatcatggg cggcacttcc aagctgcgcg gtctgctgga
     8161 gtatatccag gaaaaggtca gcatccccac cgaggtcttc atgcccttca tcaacgtgga
     8221 gatgccggaa aaattcatgg ataaaaaaga ccctcagctg gcgctggcta tcggcctggc
     8281 catgcgggcg gaataggata taagtatgaa caacgcgttt tacttcaaaa taaacctcaa
     8341 caaatacggt gatctcaaga tccagaccga gcgcgaaaag aagaacttct atcaggctat
     8401 agccgtgttc gtgctgggca ccttgatcat cctgggcgtc catttcgtgc tgtcttcccg
     8461 tctgggagcc aaggtggata gccgccgcaa gttcctcaag gaaaccgaaa gacaacttga
     8521 gagctttcag accagcgatg actatctctc cagccaggac ctcgatcgcc tcgaacaaac
     8581 cttcaacaac cgtatcttct gggccaacaa gctggtggcc ctgtctcagg aaatcgacaa
     8641 caagctggcc gtgcgcaagt tcaccttcgc caatggagtg ctgacgctca acggcatcac
     8701 ccccgtggac aacaacgtgc gtgaactgga cctgatcaat gagtttatcc tgcggttgaa
     8761 atccaacccc gagatcagca acgacttccc acagatcaaa tccggcctga tcaccaaaca
     8821 gatggtcaag gatacggcga ttatggaatt cgtgatcgag tgttacagca aggaagtgcc
     8881 gagtactccc ggaggtgaac aacgatgaga gaaaagtaca tcgtattcat cctgctgatg
     8941 gtgctcattg ccgtgttgat cttcacagcc agcgccaata acctgcagtc caacctcaag
     9001 aaagtcagag agtatgacga gcagatcaag gtagcccagg agaagctcaa cagcgcccgc
     9061 atcatggacg agcagctctc ccagttctct ttgatcatag acaacagcct ttcccgttcc
     9121 gggggcttca cctttgacga gatcaacgag tttaagaagc ggatcggcga gcttgcccac
     9181 gaacgcaaga tctccatcaa caaactgtcc gacagcaaca agttctcgct gcccggactc
     9241 cttgaaacca cctacaatct ggagatggaa gccaccttcg tgcagatggg gcaattcctc
     9301 tccgatttgg aagctctgga caacatcatc aagatccatg ccatcgacgt cactcccgcc
     9361 caggtagaag acggcaacaa gaagactgtg gataccgccg tctccaagta tcggatcatg
     9421 cttgagctct ccatcttcaa ggttaaaaag gaggcctaag tgcagctgag atatgtgaaa
     9481 gacttcgcca tcaccttgat cgtgatcatc ttcctggttt tgggcatccg cctctatacc
     9541 aactacttca aggccaagga cgtgccccag caatcgatcc actccaagga atccgtctcg
     9601 gacaccctgc tgagcaagat caaaggcatc gaaaagtcca tccaggaccg caagaccttt
     9661 gtgttcagca tcaaccgcga tcccttgcgc cagggcaaca tcatcaagga tagactcgat
     9721 ctggaaagag agtttgtaga taacctcaga aacaccttcc gcctctcctc cacgtcgatc
     9781 actgaaaacg gcaacaagat agccacaatc gagtatcgtg accaaaacta ctatgccact
     9841 atcggtgacg tggtggaagg ccggcgcatc acagagattg gcacagactg gatccgctat
     9901 tacttcaacg gctccaccta taccactcag gtagcgccac gtccgcctat gccggatttt
     9961 agcacgatca atccttctga actccagtct ggcaactttt agatcaatat aatatggaat
    10021 atatgggaga accaatgaga ttaactccaa gctcaaaacc tttcctgcgg atcatcctgc
    10081 tgctggccat ggcctggctg gcggtatcga tgatggcgca gtccaacaaa ctcaccacca
    10141 aggtgagcta taacgccgac gacgccacgc tgtcttcggt catgaacgcc ctggcccgtc
    10201 agagtggcac caatatcgtg ctggctgtcg atcccggtca ggctggcgac aagaaagaag
    10261 aacgccgcgt caccatcaat atcaaagacg ttccgatcga aaccgccgtg tccctggtag
    10321 ccagagcttc tggccttgcc tatcgcgtcc tgggcgaaaa caccttcctg gtgggcaaca
    10381 aacaaaacat catggaagaa tccggcgaac gcagcaacat tatctacctc aaaaacctgg
    10441 acgcccagaa ggtggcaagg tcccttgaga acaccaacgg cgtgatcgtt cctctggaag
    10501 gtcaaaacgc tatcatggtc tatgccaacc ccgagacctt caagaatatc caggacctgg
    10561 tcaccaaaat cgatctggaa cagaaacaga tcgagatcag ggtccgcctc atcgagatcc
    10621 atctcagcaa cgccaaaaag atgggtatcg actggagccg cctcaaccac ctcaccacca
    10681 tcctggctga ggaccccacc aatgcccgcg gcagcggcct tccctacaat tataccgatg
    10741 tagatggcta ccttcctcac ggcgacccca ccgatttcga acagcttcct gacgagcagt
    10801 atttccagcg catcaacggc ctgaaagaca ttggtcattt cagccgccag ctcaccgcct
    10861 tcgatatcac catcgactgg ctgctggaaa acaatgccgc caagctgctt acagacacac
    10921 gcgtcaccgc tctcaacggc accgaggcca ccatccacat cggtgaagtg gtacccttcg
    10981 tggtgaccga caacgacaaa cagatccagg tggagcgcga agaggtaggc atcattctca
    11041 aggtcatgcc ttccgtgaat caggatggcc tgatcaccgc cactatcgct cccgaagtga
    11101 gctccgtgac tgatctggtg ggtggttatg tgccccgcac caaagtgcgc cgcgtcacta
    11161 cctccgtgac cgttcccaac gagcagaaaa tcatcgtggg cggattgctg aacagcaaca
    11221 tcagccaaaa gacttccaag cttcccttcc tgggagacct tcccttcatc ggcaggctct
    11281 tccagcatcg ttatgaagtg gttgagaaca ccgacctgat catggagatc actccccgcc
    11341 tggtctcgat caccgaaccc agcccggaac ccaaggtcga cgagcgtctc acccgtgctc
    11401 tgatcgaata cgaagacaat cagaattccc aaaaataagg ggtgaacaat gaagactaaa
    11461 cgcatcccca ttatcactat tatggccttg ctgcttgtca tgctggccac ctcatgcgac
    11521 cggcgcaaca tccccgtcgt tatagaccag gagccggtgc ccatctcatc cgagcgctat
    11581 atcgacgata tatccgccac cccggatatc atctacgcgg attatggcat cacctactct
    11641 ctgatcaaag tattggtgga agatggtgaa ggctttggcg ttcccagcca gattgtgagc
    11701 ttcaagacca ctcttggccg gatcctgacc aacgtgccca ccgacagcac cggtgtagct
    11761 accactactc tgtgggatgc tggtgtggcc ggtgaagcgc aggtcactgc cgtggtccgc
    11821 aaatactccg acgaagaccc cactgtggtc ctctccgaag caacccagac cgtgacggtg
    11881 aacattctcc ctgtgccaga tatcgatagc gtcactctgg aattccagtc cactctcaat
    11941 cccttcccga tgaccgtcat gcagaccacc gaggtgagcg cggccgtcaa aaacgtgctg
    12001 gacgagtatg tccccaacaa tacgctggtc accttcacct gctccaaagg cagatttgtg
    12061 gacgcggctg gaaacgtgct gggcgtatca gtggtcgcca aaaccaccaa tggcagggcc
    12121 acggtgaact ataactccgg cgctacttcc accacctcac ccggcatgga aaatgccctg
    12181 atcagagcag ctatcggaac cgtctatagc gagcgggaag tggtcatcag ggccggtgct
    12241 ccggctgtga tcgatctgcg ctcctatgtg tttgccgatg gcgactccac cgagacccag
    12301 accaatccag tggacagcgc caatgagatc aagctctatg ccactctcaa cgatctgcat
    12361 ggcaatgcct gccagaccaa gcctgttaag ttcagcactg atctgggtac ctttatgaat
    12421 actacccagc tcatcactgt gaacacaggt gccgacggca aggcaatcac aagactgatc
    12481 ccgggcctcc aggctggcgc ggccactatt caggcatccg ccaatggcga caccctgatc
    12541 acccaattga tcttcagcgt cacctccagc gagattcact ccatcagctt cactcaggat
    12601 acccagatcg acctcagcgt cgccaacact ggtggcaccg aatccgccat cctgagagtc
    12661 aaactgcgcg atatcaacgg caacctgatc gacacccaaa aacaggtctg gttcaggctg
    12721 ctgtggaaca accccaatga cccccatccc gaaggagcca acctcaacaa ccagcctccc
    12781 aatgattcgg tgatggtcat gtccaccgga ggtgaggccc aaatctcggt aaacagcggc
    12841 cccgaatccg gcgtgctggt agtgagggcc tctcatatca gagaatccga cagcagctgg
    12901 atccgggcta ccaaggccaa catcctcatc catgccggtc cacccgctac aatcacgcca
    12961 ttcattggcc gcttcaacac cggtcagaac atgggcggtg gtctgtggcg cgtgattgca
    13021 ggcgcggaag tgtatgacat ccatggtaat cccgtcgata aaaacacctc ggtgttcttt
    13081 gagatcatca acaacaccac cagctgtacc attgaaggta acggctatgt gggcaacgtg
    13141 agtgtggagg acgattcaat ggcagccatt gcctatacga tcgtcaccta ttccggtgtt
    13201 cacaccaacg agatgatcac tatccaggcc accacaggtt ctggcacagg ggccatcgtg
    13261 caaggttttg ccactttgcc tctgcctttg aatgatcccc gtttcgagat ccaggccaat
    13321 cctcaatcgc taaacttcgg cgatacatcc ccggactgga agtccaccga gatctattgc
    13381 gtgctgaccg acggccaggg ctgtctgatc ggcaatcagg agatcatgct ggtgagcacc
    13441 aagggacagt ttgtggccat accaggtttc aacaatgact atcccgccgc gccggtctgg
    13501 atgatcaaga ccgacgacgg cagctatggc actggcaact atgccggctg ggcatggggc
    13561 cagatcaggt tccaccggct ggaatgtccg gctggagacc ctcaggccca atctcccgga
    13621 cagacgaccg gctccatcat tggccgtatc ctgggtacgg cagtcacaga tcagacggac
    13681 gtggccattt atcgctactg gacgcctgct cctcccttct aaacaatagc taaatatata
    13741 tggcgccggg agatcccggt cttccggcgc tctttttcca ataacactat taaactggaa
    13801 ctcgacccta cgaggagatt tatagacccc aagctggccc caggccatta gatttaagaa
    13861 tacaacacag aggatgagtg aatgacctat aatccccagt ttgcccgcat cggcgaagtg
    13921 ctggtgcatg agggctttgt aaatgaagat caggtaaaag aggccatgat caagcagacc
    13981 aatttcggcc tgaaattagg cgaaacgctg atcaagcttg gatacctgag agaaaaagac
    14041 ctgctaagcg ccctgcagat gcaattgggc tatgacatcg tggaagaccg cgaactgatg
    14101 gacctcgatc tggaaatcgt gtctctgatt cccgagccct acgcgctgga aaaccgcgtg
    14161 attgctctcc gggaagaagg agacggcatc gtggtgggaa tcgccgatcc ggaaaacctc
    14221 accgtcctgg acagcctcaa aaaactgctg ggcaaaaacg tcaaaccaga gctgattggc
    14281 gattctgccc tgcatgatac catcgagaaa tactataaaa gcatccgcac tacatcccag
    14341 gtggaagacg ccgtgggcgg atttgacttt gtggccgtgg atgaagacga aaacgaaatc
    14401 accatcgcca acgcttctac ttcagccgac gctcccgtgg tcaaattggt gaacctgatt
    14461 atcaatgaag ccatcaaagc cggcgccacc gatattcaca tcgaacccct catcaagacc
    14521 tcccgcatcc gctaccgcat cgacggcgcc ctgcgtgaag tgatgacccc tcccatcggt
    14581 atgcatgccg gggtggtctc gctcaccaag gtgatgtcca agctcaacat cgcggaacgc
    14641 cgtctgcctc aggacggtca catcgcgctc aagacttcca tgaagagcgt ggacgtccgt
    14701 gtgtccatta ctcccaccgt tttgggtgag aaagtcgtga tgcgtctctt ggacaaaggt
    14761 gactttggtt tcaaactcgg caccctcggt tttgaagaag aggatatggc gatcttcgcc
    14821 aagatcattc gccgccctta tgggatcatc atcgtgtccg gtcccaccgg tagcggtaaa
    14881 tccacctcgc tgcacgccgc gcttaaagag attcaaaata tcgaatccaa tatcatcacc
    14941 gtggaagacc ccgtggaata tcgcctcgaa ggcatcaccc agatcgagac caaagaacag
    15001 atcggcctca ccttcggatc agctctccgt tccgtgctgc gtcaggaccc ggacatcgtg
    15061 ctcatcggtg aaatccgcga cgaagagact gccgacattg cgatcaaatt ctcgctcacc
    15121 ggtcacctcg tgttttccac cctccacgcc aacgacgctc cctccaccgt cacccgtctc
    15181 atcgatatag gtatcaagcc ttatctggtg ggctcttcgc tctcgttggt gatggcccag
    15241 cgcctcatcc gcaagatctg cccctactgc atcgacgatt acgctcccac cgctcaggag
    15301 atcagcgatt gcgggctcac cccggaagag gcttcccaga tcaaattcaa gatgggcaaa
    15361 ggctgcgtcc actgcgacaa cacaggcttc tcgggccgta ccggtatctt tgaactgctc
    15421 accgtggatg ctgaaatccg ccggctcgtc tatgaaggcg gcaatcagga cctcattcgc
    15481 gacgccgctc tgcgcagcgg gatgcgcacc cttcacgacg ccgctatttc taagatgaaa
    15541 cgtggcctga ccaccatccg cgaagtcatc aaaatgaccg taatcgaata agtttacaag
    15601 ataaaaggta agcactatgc caaatttcgc gtacatcgtt aaggacgcca aaggcgccag
    15661 agtggaaggc attctaaagg ccgatactct ggacatggcg gtggacaaac tggccaaaga
    15721 aggcagcacc atcatcagcg tcaaagccgc tgccgaaggt gccttcaaag gtaagctctc
    15781 cctcttcgac aaactgatgc tccaggtcta taaatggcgc accggcgtgt cgctgaggac
    15841 cctggtgttt ttcacccggc agctttccac catgttttcc gccggtctca ctatcgaaaa
    15901 ggccatcacc gacctcgaaa aggaagagaa aaacaaacgc ttcggacgcg tcctgcgcaa
    15961 gctatcagac gacatccgca aaggcttttc gctctctgag gcgatggaac agcaccccgg
    16021 ggtattcaat cccctctatg tggcccttgt gaaggccggg gaagtctccg gtaccctgca
    16081 caccgtgctg gatgaactct ccgactatct ggaaaagatc gaggataccc gccgcaaggt
    16141 gatgtcttcc atggcctatc ccatcttcat cctcgtcttc ctgggctttg tgacctgggc
    16201 cctcttctac tacatcattc cgctcttcgc gggggtgtat ggagacttcg gtgccgatct
    16261 gcccgctccc accgtcatgg ctatcaaggt gtccaatttc attgtggcca atgtgtttgc
    16321 cactatcctg atcgtgatcg ccgctgtcat cgggctcttt ttggtgtatc tcacggatcg
    16381 cggacgttat gccatggatg ccttcaaact caaaatcccc gtgatcggca gagtgctctc
    16441 caactccatc atgagcaaat tctcccgcac cttctccatc ctcatggctg ccggggtgcc
    16501 gatcatggac accatggaac tcaccgaaaa cgtggtccag aacgccgtcg tggaaggtgg
    16561 tgtgcgcaaa gcccgtgtga tggtgaaaga aggctacgga gtggccaatg ccttccgcaa
    16621 gacgggacta ttccctccca ccctgctgca gatgattgcc accggtgaag aaaccggaga
    16681 tatggacaag ctgctgggta aagccgctca gttctatgag aagctggtgg atagcgtcat
    16741 cgaccgcctc acctcactca tcgagccgct cctgatcgtg atcatggccg ccatcgtggg
    16801 tggcatcatc gtgatcatct accttcccat cttcgatctg ggcgacgcca tcagccaggg
    16861 ctttaattaa tggaaccgca aacgttcgcg cttgtctgaa tatctaaacc catcaataat
    16921 cacataatgc agggctgaat tcgcaatcaa gcgctttcag ccctccagct ttggcaggta
    16981 aacctatgat cgatcagact caagccaccg gatcagagct gctcagcctg gtccgcaaga
    17041 tcatgcacga cgtcaatact ccgctctgcg tgatcaacct ctccctcaac cgtctggaaa
    17101 ccgtgatgga cgctcaacct catcccgaac tcagcaaaag ctacgaagag atcagcgcgt
    17161 ccgccgataa actgctggaa atcatgtcca ggctggacca ggcccgagcg atgatagagg
    17221 agagtggcat ctgagctaag atcatcatcc ggatcattta acattccaag gtcatttatc
    17281 ccatgaaaag gagactacta tgaaacaagg cgcgctaatc gttttactgt tgctgctcgt
    17341 ggcattgggg ctggaggcag ctcctgtact ccccttgtct gtgctacggg ttccgcacgc
    17401 atgggtcctg ccccata
//
LOCUS       DAOSFN010000041        17313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2678_length_17313_cov_10.100881, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000041 DAOSFN010000000
VERSION     DAOSFN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 17313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17313
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2678_length_17313_cov_10.100881"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..843)
                     /locus_tag="PLO35_04190"
     CDS             complement(88..843)
                     /locus_tag="PLO35_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78897.1"
                     /translation="MKRLLLISLIALCLMDLSAQSDLSSSIRDGAQLNEDQKALSELE
                     KLKAEGHNHPDLYFNLGVCHNKLGRPGLATLYYLKTLSLDSAHPEARHNLKLLQDLSP
                     SKANQENNPFLVQLLYDILAWLNHARLAILVLVFLGLTVLCAHWLLHYSPDAEKGPPI
                     LLLSICTLLLVMASTVLIVKQQRSSRDNRAVVTVPLATIYKDEGTLSSSENLPESSIV
                     RIISQRRDLYQARLQDGSLVWIKRADLTRLQDI"
     gene            complement(840..2624)
                     /locus_tag="PLO35_04195"
     CDS             complement(840..2624)
                     /locus_tag="PLO35_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOH78898.1"
                     /translation="MKQLWLPFFILFTILGPLDAQSKVSVSNSVNKQVLYQSDLLLYN
                     LRVSASGSVQPEEPPAPNVSGLRFHTQTSSRESRANIVNGRLETSSSVTYSYYYYPTR
                     SGKITIPAQSVRVGRSVYRTNPITVEVKPDISSPPSRPSPSRPQRPSSPSYDPFGSFL
                     PPPPEAEGESYIVCLPETQSVYRSEPAIVSYYLYTTEDVRSLNLTEERDFDGYGKAVF
                     EQPGSLDFERVEYKGRSFRRSLLKRLVLYPHSTGTLKAPEMAGSIRLMTFMYQNRNLS
                     SRPASITVRELPSGAPAGFGGAIGSFAITENYSSQSVRLGEAIVYTVQISGQGNFNQF
                     TAPSMSSDARVQISSPQVRDQLNAGTKGTRILQYTIIPRVKGEIRLPRFKFSWLDSNS
                     GKYLSFNGREAVLNVKAGTSTVTTPDLDKMLDKLSMFSRIERDAYPPFRPWHLRWWFW
                     LLAGLMLLSLPVSAVRAKRKQARFRDPVGWKNREAAARLRDSFNQAKSLAASSSKDFY
                     QVAGNCLSAYLAERYDLTAHLGTGELATELASKGLSDDLISQIQQFLKDMQAALFAPS
                     ANSDNAIATDLPKLEKIVRALTEAGGKA"
     gene            complement(2621..3265)
                     /locus_tag="PLO35_04200"
     CDS             complement(2621..3265)
                     /locus_tag="PLO35_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78899.1"
                     /translation="MKPRTPSPNNHAKTRSWIRQALLVLIALILIYFALEFLLRRDAF
                     YNSIASGLYKLGRAEQASSLWEKRLDPDDADPVPEANYGKNAFRQGQYSEADKHISEA
                     LRDDPENPVWNYDQGNTQYREEKLDNALESYKKAMLADPSDQDAKSNYELVLNRKGYK
                     KPKPEDHQDEEKEQDKDPVPPSDYENTLDALDQKESNDRQEPGRPQEEPMERWW"
     gene            complement(3262..4236)
                     /locus_tag="PLO35_04205"
     CDS             complement(3262..4236)
                     /locus_tag="PLO35_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH78900.1"
                     /translation="MQFYNSGLLLLLLLIPVLLALLVRSRYRSRKRFASFADEHILGH
                     YLQRISPFNSTMKQLLLIAALGFVVLGLVRPQWDHELSDLESQGLDLIFCLDISRSMD
                     ASDLAPSRLERAKLQIHSLLDRMQEDRVGIIAFAGAATLECPLTDDYESVRMVLNSLN
                     TQSAVKTGTDLGEAFSLAEKAFDTASGSNILVLVSDGEDLEQRGGNRAARLAASGVKI
                     FAIGVGSPEGAVIRHPLSGVEAFTKPDLDFLAKVASEGQGQFYNLIETETATAQIITS
                     IRESSEGKRHGNRVNGLKDQYHFFALMAMLLLIAESLIIPLRRESRSQ"
     gene            complement(4240..5235)
                     /locus_tag="PLO35_04210"
     CDS             complement(4240..5235)
                     /locus_tag="PLO35_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH78901.1"
                     /translation="MLQFAKPWLLILLLFIPLYWMIWRQRQKRHQLRVPFPRLMEIAQ
                     TGRTGKFWKYFYPGLRTLILLCLVLALSQPRWGRGVRDVKQQGVDIVLAVDISGSMLA
                     EDFAPRNRLSAAVQVAMKLVRNRPNDRFSLVAFSEYALTQSPLTFDHHSVLDQLNRLE
                     VNMQASGTAIGMGLAKAVARLRKSTAKSKVVILITDGVSNTGEIDPISAANMARAYGI
                     RVYPIGVGSNGLVDFPFDDPLLGRVYRKTLIELDMQTLDRIAALTNTGKASLATDTGQ
                     LQSIVDQIDAMEKTEYRLRLHYLWSEKFMLLLWISFGLLLIELLSRLHFKPILPE"
     gene            complement(5219..6154)
                     /locus_tag="PLO35_04215"
     CDS             complement(5219..6154)
                     /locus_tag="PLO35_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78902.1"
                     /translation="MRSLLAALMLLLGIACLSADAGIYQSLENDDNLSVGDRFLFNIK
                     AEYSINRVVVPDTLSNFKVIDSQRVSEAGSPAWYRLTIVPLLPGYHSFPALKVEPVRP
                     DNSVAYTDRFRVNVIPVRAESDTTLVDIKPPLAYPMQAPGWLYLVLAGLVPLLALAYF
                     LSKPGKAKTEAPVQAPAPAPPVKPSNWKVALEQLDRLMTEDLLAQGMVALHHFQLAMI
                     LRSFLEKEFRIAALEMTTSELREAMQRVNVQRSSEVNFFLNFCDRAKFAKHIPTPEDS
                     HGMEQWLRDYLLGFEILAAQRMLDKPRGPANAPVR"
     gene            complement(6151..7044)
                     /locus_tag="PLO35_04220"
     CDS             complement(6151..7044)
                     /locus_tag="PLO35_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOH78903.1"
                     /translation="MLTQTPADILRRVRKIEIHTRTKVSEVFRGEYHSSFKGQGLEFA
                     EVREYQPGDSYRSIDWNVSARFGYPYVKLFQETRELNVIFLVDVSASQDFGTRIMLKK
                     EKLAELVALLSFSALSNNDKVGMIMYSAILEKYLPARKGRNRALEMLRDILYLEPKDK
                     RTDLKAAFRYADLVLKKRSVIFVLSDFLDHNYSEPLRMLARKHDVIAMQVLDEAELKL
                     PRAGVLNCYDPETGAAQYVNTADPVLRDIYEKQTRAAQLKLTEELQKARVDHLLFTAK
                     DEVTATLRDFFERRKRHARIR"
     gene            complement(7051..8037)
                     /locus_tag="PLO35_04225"
     CDS             complement(7051..8037)
                     /locus_tag="PLO35_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOH78904.1"
                     /translation="MSIEEIHRRVLDSSAILDEVREEIAKVIVGQDAVVRRLLIGILV
                     NGHVLLEGVPGLAKTLIVSTLAQVFDASFRRIQFTPDLLPADITGTLIYDQRTGEFNP
                     KRGPIFSSFILADEINRAPSKVQSALLEAMQERQVTIGDTTYPLPFPFFVMATQNPIE
                     QEGTYPLPEAQVDRFLFKLRIKYPSLEEERRIMDRMVIDQPIPVDKVLTPAKVKVMQD
                     VITDIHMEDKLKDYILHLVQATRHPERYPRVSSMNGLIEYGASPRASISMARAAKAHA
                     YLEHRGYVIPDDIKAIGRDVLRHRIILSYEAEAEQLTADEIINRIFDEIEVP"
     gene            complement(8197..8394)
                     /gene="rpmB"
                     /locus_tag="PLO35_04230"
     CDS             complement(8197..8394)
                     /gene="rpmB"
                     /locus_tag="PLO35_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOH78905.1"
                     /translation="MSRICDICGKSAQVGNHRSHAVNATKRRFYPNLHEIRALLPGGV
                     KRVKVCSSCLKANKVRKAVER"
     gene            complement(8459..9574)
                     /locus_tag="PLO35_04235"
     CDS             complement(8459..9574)
                     /locus_tag="PLO35_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOH78906.1"
                     /translation="MTEQQNIDEVGIFHGLSEDVVNGFAKDLQRVWHKKGEAIIMENT
                     QGDNLFYICHGRVEINKGLENEETPFAQLSILHPGEFFGEMSIINDEPRSATVVALDD
                     VELLVIPRDVFVNITFSHPVVMFNLIRTISGRLRNTNDKFIELMNQMISKNRLMAIGL
                     AASKIIHDIKSPLTVIVLTAQLLENLYPDGAEYTDSIIKQTKLVDQMVREILDFAKGT
                     EIEPLIQKVDLDAYFKDLKDTYGTSLKSRDINFVVENKVSELVHFDENKIKRVLLNLL
                     KNSSEALTDKGEIRIVASLSAGWLQISLIDDGPGIPEEIRNDLFKPFVTSGKAHGTGL
                     GLAICKKILQEHKGRLEYIPVQPHGARFDIRIPQSMK"
     gene            9807..12272
                     /locus_tag="PLO35_04240"
     CDS             9807..12272
                     /locus_tag="PLO35_04240"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOH78907.1"
                     /translation="MTKDLNDMITSLRLEGSSEGIVKRFVEHIKYQRGKDQFSATPQD
                     CFQAFATVIRDIMLDRWLVTQPEEYAKNSKRVYYLSMEYLIGRSLGNAILNLDLGLQS
                     LEALKKLGFEMSMLEELEWDAGLGNGGLGRLAACFLDSMANLRIPAYGYGIRYEYGIF
                     FQHIKNGEQTETPDNWLRYGSVWEIPRPERLFPVHFGGRIVNTVNDQGGGQPSWVPAE
                     SVMAMAYDYLVPGYQNGYVNTLRLWSAKSTRDFNLQYFNEGDYVQAVADKNSSEMISK
                     VLYPNDNNMAGKELRLKQEYFFVSATLQDVLRRFAKKHKDWSAFSGQAAIQMNDTHPA
                     IAVAELMRLLVDEKHLNWEVAWEITKATLAYTNHTILPEALEKWPVQVLERVLPRHLQ
                     IIYEINHRFLYEIRLTRKADDPTLRRLSIVEEEPVKSVRMAHLAIVGSHSINGVSALH
                     TQILKDKVFKDFYQYYPEMFNNKTNGITPRRWLMLANPLLADLLDDTIGTAWRQDSRK
                     LSKLNALLDDSTFLDELEKVKHQNKLDFAAYHQDVFQHKLDPDSIFDFQAKRFHEYKR
                     QLLNALHIIHLVLRIKDGETAGLVPHSFFFAGKAAPGYYMAKLVIRLICAIGTYIDRD
                     RDLSKYLKVVFLHNYRVTLAERIMPAAEVSQQISTAGTEASGTGNMKFAINGALTVGT
                     LDGANIEIRDAVGPDNFFLFGMTAEQVKQLKQTGYHPYGIAHGDPTIKRIIDFIGSGE
                     LDAFHPGLFQPILNSLLYQGDEYCVIADLADYTRVMEAVNDAYPQRRQWNRMSAANIA
                     NMGLFSSDVTIEAYAREIWGVIK"
     gene            12621..14432
                     /locus_tag="PLO35_04245"
     CDS             12621..14432
                     /locus_tag="PLO35_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78908.1"
                     /translation="MINANSAVVRLRFALLLILIIGIGASALQAQVLFSFNVNDLEAY
                     DRSGNWIYDADGPGFPNHDAQWFTFSPVVGYPLHYFAERYIHEGVTTTLTCLAENVAQ
                     ADNPGQLTMSFTQFNLVSFKRINTVNPLAPWNIPGQSGDERVYANAGGTISYNGTPVL
                     TMTDATFVITTPYPNQAQIQALSPMLATWTGDIGTGAPQTGFGYGDLDLAHSDPAWIP
                     LFAASNYKIELQMAGITSVVTPTAGYFDFTLNVLPATTSYIGGNVVVDLGNLPELHPI
                     PGTDATLGAIAGTPGGQNGDLRTLFLAEIGVPPSGNIPAGFNYSTKKHWELGGTLQTF
                     NVNVSLNLGLDDFQAKAPSDWRIVYRSNASQDWALWPDQMLSGPTTITAQNVTDFAYF
                     AVVSPFDETLPVVLSSFAAYVNSNSLAELKWTTASETDMTGFKVYSSSNTQLGSAICL
                     TPVVIPASNTSDGAHYSYTATELEGPGTFFFWLEALSMNGQSDFYGPVSVNLPADPGT
                     PVLPPRSELGIAYPNPFRSGSMTRIAVDIKEGETGTLEIYNIAGQKIKSQTMAAGSHN
                     IDWDGKDASGRQVASGVYYYKLSTGSYTQSRKLVIVK"
     gene            complement(14505..15944)
                     /locus_tag="PLO35_04250"
     CDS             complement(14505..15944)
                     /locus_tag="PLO35_04250"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH78909.1"
                     /translation="MEHLIKDIITKLNLPGVTFADARITYTDSEGIRFMNGFLRDYGS
                     SKASPALGVRVLINGCWGFAGSGDLSKASIDRVIKTAITNAKSGSGFIKDPVRFPMLP
                     ATIADYHFEPEIDPFLMDRAEKLEYLGRLASAIKPQGKIVHSFVAAQFQRQEKYYANS
                     EGSYSHTTVYNTLPYMEVIAADGKQMQTRTWPGHMSAGRGGFELFYQQMFEENTERII
                     KEATELLDAPGIEEERADIIIGGGHLALQLHESVGHATEADRIFGMEISYAGKTFVKP
                     AMLGSYQYGSEAVNIYSDSSDPRGMGYHPMDDEGVPGRRVDIIKDGILKDQQTSRHIA
                     HLLGLEPSSNMKASFADDFPLVRMTNFCLAPGSGSLNELIESTEHGYLLDFTKTWSID
                     DNRNNFQFTTEIGYRIDGGKITGIVKEPTYFGITKDFWNACDKVCGEQEWKFHGTFHC
                     GKGEPGQAMLLSHGVAPARFRNINVSVKI"
     gene            complement(15953..17278)
                     /locus_tag="PLO35_04255"
     CDS             complement(15953..17278)
                     /locus_tag="PLO35_04255"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH78910.1"
                     /translation="MDYLNYLASALAGQSGLKYELSSQNWSTDFLRFFHSQTNYNISK
                     DKLSLSVGLWRGKKSYRFGIDDPNQDKIDLALRDALAIIDQLPEDPDFVDLETDLSIA
                     PPRQLNNNIELIPLDTKTAILKELAEAAKPFDFEIYGTFICNYVQHRIVNSNGLDKSS
                     SSSPIYLEVKAVHNQSQITVLETFGGDDFNRFDPQDFKARLLQKIAYCANPVVDVEPG
                     YYDVILAPRCVAEYAQYLCWGMSAQAFDEHSSFFEDKLDKQVFPEWISITDDPRDPDL
                     IGQDYGSNGHIYNPLKLIDRGVFKAFLCNNYYAHKTGLPKNGNTASCLSIATGDKTLD
                     EMIGSVRRGLYISSLHYMNFINMKETSLTGLTRDGTFLIEDGKITRVVNSLRFTERIE
                     RIFNHVQALEDRGYTIPFSENYEEFDIESVKAPHALVKDFNISSSTHTI"
BASE COUNT         3743 a         4530 c         4899 g         4141 t
ORIGIN      
        1 ttcccgaaga gcattacgga ctcattacgg acttagtccg taatgagtcc gtaataagtc
       61 cgtaatgcca tgcggggagg aggcggctca gatatcctga agccttgtta gatcggctcg
      121 cttgatccaa accaggctgc catcttgcaa gcgggcttga tacaggtctc ttcgttggga
      181 gatgatcctg acaatcgagg attcaggcag gttttccgag gaactgagag tgccttcgtc
      241 cttgtatatg gtggcgaggg ggacagttac cacggcgcgg ttgtctctgg aggagcgctg
      301 ctgtttcacg atcagaacgg tggaagccat taccagcagc agggtgcaga tgctcaaaag
      361 cagaatagga gggccttttt ccgcgtcggg tgaataatgc agcaaccagt gagcgcaaag
      421 gactgtgaga cccagaaaaa cgaggactaa tatcgccagc cgcgcgtgat tcagccaggc
      481 caggatatcg tagaggagct ggaccagaaa gggattgttt tcctggttgg ccttcgaggg
      541 gcttagatcc tggagcagct tgagattgtg cctggcttcg gggtgagcgc tgtccagaga
      601 aagggttttg aggtaataga gcgtggccag accaggtctg ccaagtttat tgtgacatac
      661 ccccaggtta aaatagaggt cgggatgatt atgtccctct gccttcagct tctcgagttc
      721 ggacagtgct ttttggtcct cattgagttg ggccccgtcc cggatgctgc tgctgagatc
      781 gctctgggcc gataaatcca tgaggcacaa ggctatcagt gatatcagca gtaagcgctt
      841 catgcttttc ctcctgcttc tgtgagtgcc ctgacgatct tctccagttt tggcagatca
      901 gtcgcaatgg cattgtcgct gttcgcggaa ggagcaaata gggccgcttg catgtccttc
      961 agaaactgct ggatctggga gatcaggtca tccgaaagac ctttagaggc cagttccgtg
     1021 gccaattccc ctgtccccaa atgagctgtc aggtcatacc tctcagccag ataagcgctc
     1081 aggcaattcc cggccacttg atagaaatcc tttgatgagg aggccgctaa gctcttggcc
     1141 tgattgaaac tatctcgcag cctggcagcg gcttcacggt ttttccatcc taccgggtcc
     1201 cggaagcggg cttgtttgcg tttggcccgc acagcagaaa ctggcaggct caacagcatc
     1261 agtcccgcca gcagccagaa ccaccagcgt aaatgccagg gccgaaatgg tggataggca
     1321 tcccgttcga ttctggaaaa catgctgagc ttgtccagca tcttatccaa gtccggagtc
     1381 gtgaccgtgc tggtgccagc tttgacgttc agtactgcct ccctgccgtt gaaactgaga
     1441 tacttgccgg aattggaatc cagccagcta aacttgaagc gcggtagcct gatctctccc
     1501 ttgaccctcg gaatgatggt gtattggagg atccgggtac ctttggtacc ggcattgagc
     1561 tggtctctca cctgggggga ggatatctgc actctggcat cgctggacat ggagggcgcg
     1621 gtgaactggt tgaaattgcc ctgaccacta atctggacgg tgtaaacgat cgcttctccc
     1681 aatctgacgc tctgggatga gtagttttct gtgatagcaa agcttccgat agcgccacca
     1741 aagccagccg gggctccgga aggcagctcc ctcacggtaa ttgaggcagg cctggagctc
     1801 agattgcggt tttgatacat gaaggtcatc agtctgatcg agccagccat ttcgggagct
     1861 ttgagggttc cggtgctgtg gggatagagc accaaacgct tcaggaggct gcggcgaaag
     1921 cttctgcctt tatattccac ccgttcaaaa tccagagaac ccggctgctc aaacaccgct
     1981 ttgccatagc cgtcaaaatc ccgctcctcg gtgaggttta aagagcggac atcctcagtg
     2041 gtataaagat agtaagatac gatggcgggt tcgctgcgat aaaccgattg ggtttcgggc
     2101 aggcagacga tatatgattc gccctccgct tccggaggtg gtggaagaaa ggacccgaaa
     2161 ggatcatagg atggagagga tggtctttga ggcctggaag gagagggcct ggaaggaggg
     2221 ctggagatat caggtttcac ctctacggtg atggggttgg tcctgtaaac tgaacgcccc
     2281 acgcgtacgg attgggctgg gatggtgatc tttccgctgc gggtgggata gtaatagtag
     2341 ctataggtga cggacgagga ggtctcgagg cgtccgttaa cgatattggc acgtgattcg
     2401 cgtgagctgg tttgagtgtg aaagcgcaga ccagacacgt tgggggcagg gggttcttca
     2461 ggttgcacgc ttccgctggc tgaaaccctc agattataga gcaacagatc actctgatag
     2521 agcacctgtt tgttgacgga gttgctgacg ctcactttgg attgggcgtc gagcgggccc
     2581 aggatagtaa aaaggatgaa gaaggggagc cagagctgtt tcaccaccag cgctccatag
     2641 gttcttcctg aggtctgcca ggttcctgcc ggtcgttcga ttccttttgg tccagggcgt
     2701 ccagggtgtt ttcataatct gaaggcggca cagggtcctt gtcctgctcc ttctcctcat
     2761 cctgatggtc ttccggcttg ggttttttgt agcccttgcg gttcagcacc agttcatagt
     2821 tgcttttggc atcctgatcg gagggatctg ccagcatggc ttttttataa ctttccaggg
     2881 cgttatcaag tttttcctca cgatactgtg tgttgccctg gtcgtagttc cacacggggt
     2941 tttcggggtc gtccctgagg gcttcagaga tatgcttgtc tgcctcggag tattggcctt
     3001 gccggaaggc atttttgccg tagttggctt ccgggaccgg atcagcgtca tccggatcga
     3061 gacgcttttc ccacaaggag ctggcctgtt cggcgcggcc cagtttgtag agcccggaag
     3121 cgatgctgtt gtaaaaggca tcccgcctca ggaggaattc cagcgcgaag tagatcagga
     3181 tcagggctat caggaccagc agggcctggc gaatccagga tcgcgtcttc gcgtgattgt
     3241 tcggactggg ggtcctgggc ttcactggct tctgctctcc cggcgcagag gaatgatgag
     3301 gctttcggcg atgagcaaca gcatggccat caacgcgaaa aagtgatact ggtccttgag
     3361 accgttgaca cggttgccgt gccgttttcc ttctgagctt tcacggatgc tggtgatgat
     3421 ctgggcagtg gcggtttcgg tctcgatcag gttataaaac tgcccctggc cctcagacgc
     3481 cactttggcc agaaaatcca ggtccggctt ggtgaaagct tccactccgc tgagaggatg
     3541 acggatcact gctccctctg gtgatcccac cccgatggca aagatcttca ctccgcttgc
     3601 ggccagacgt gcagcccggt tgccgccacg ttgttccaag tcctctccat ccgacacgag
     3661 gacaaggata ttggacccgc tggcagtatc aaaggctttt tctgccaggg aaaaggcctc
     3721 tccgaggtcg gttccggttt tgacggcgga ttgggtgttg aggctgttga gcaccattcg
     3781 gacgctctca tagtcatccg tgagggggca ctccagggtg gccgctccgg caaaagcgat
     3841 gatgcccacc cggtcttctt gcatgcggtc caggagagag tggatttgca gcttggccct
     3901 ctccagcctg gaaggagcga gatcggatgc gtccatgctg cgtgaaatat cgaggcagaa
     3961 tatgaggtcc agcccctggc tttccaggtc gctgagttca tggtcccatt gcggacgcac
     4021 cagtcccagc accacaaaac ccagcgcggc gatcagcaac agctgtttca tggtggagtt
     4081 gaagggagaa atcctctgga gataatggcc cagaatatgc tcatcggcaa aggaggcaaa
     4141 gcgtttgcgg ctgcgatagc gtgaacggac caacagagcg agcagcactg gaatcaggag
     4201 cagcaggagc agaagaccgg agttataaaa ctgcatggct cactccggta aaatgggttt
     4261 aaaatggagg cggctgagta attcgatcaa cagcagcccg aagctgatcc agagcaacag
     4321 cataaacttc tcgctccaga ggtaatgcag ccggagccga tactccgtct tttccatggc
     4381 gtcgatctgg tccacgatgc tctgaagctg gcctgtgtcc gttgccagcg aggcttttcc
     4441 ggtattggtc aaggcggcga tccggtccag agtctgcatg tcgagctcga tcaatgtctt
     4501 gcgatagacc ctgccaagca gcggatcgtc aaaggggaag tccaccagtc cgttgcttcc
     4561 cactccgatg ggatagaccc tgattccata agccctggcc atattggcag cgctgatggg
     4621 atcaatctca ccggtgttgc ttacgccgtc cgtgatcagg atcactacct tgcttttggc
     4681 ggtggatttt ctgagccggg ccacggcttt ggccagtccc atcccaatcg cggtgccgga
     4741 ggcttgcatg ttgacttcca ggcggttaag ctggtctaag acggagtgat gatcaaaggt
     4801 gaggggactc tgggtgagag cgtattcgga aaaggccacc agagaaaagc gatcgttggg
     4861 acggttgcgc accagcttca tggccacctg caccgcggcg ctgagacgat tgcggggcgc
     4921 gaaatcctct gccagcatgg aaccgctgat gtccaccgcc aatacaatgt ccactccctg
     4981 ctgtttcacg tctctgactc ctcttcccca acggggttga gacaaggcca gcaccagaca
     5041 gagcaggatc aaggtcctga gcccgggata gaaatacttc cagaattttc ccgtcctgcc
     5101 ggtctgagcg atttccatca gtctgggaaa ggggactcga agctgatgtc gcttctggcg
     5161 ctgacgccag atcatccaat acagcgggat gaagagtaac aggatcagca gccagggctt
     5221 agcgaactgg agcatttgcg ggtcctctgg gcttgtccag catgcgttgg gctgccagga
     5281 tctcaaagcc cagcaggtaa tccctcaacc attgttccat cccgtggctg tcttcggggg
     5341 tggggatatg tttggcaaat ttggcccggt cacagaagtt gaggaagaaa ttgacctcgg
     5401 aagagcgctg cacgttgacc ctctgcatgg cttccctcag ctcggaggtg gtcatttcca
     5461 gcgcggcgat ccggaattcc ttttccaaaa agcttctcag gatcatggcc agctgaaagt
     5521 ggtgcaaagc taccatacct tgggccagca ggtcttccgt catcagcctg tcaagctgtt
     5581 ccagagcgac tttccagttg ctgggtttga ctggtggggc tggagcaggg gcctgaacag
     5641 gggcctcggt cttggccttg ccgggtttgc tgagaaaata cgccagggcc agcaatggga
     5701 ccaacccagc cagcaccagg tatagccatc cgggagcctg catcggatag gccagagggg
     5761 gtttgatgtc caccagcgta gtgtccgatt ccgctctgac ggggatcacg ttcacccgga
     5821 agcgatccgt gtaggccacg ctattatccg gacggaccgg ttccactttg agagctggaa
     5881 agctgtgata accaggcagc aggggcacaa tcgtgagtcg gtaccaggct ggcgaacctg
     5941 cctcgctgac tcgctggctg tcgatcactt taaaattgct cagggtatct ggtaccacca
     6001 cccggttgat ggagtattca gctttgatat tgaagaggaa gcggtctccc acgctgaggt
     6061 tgtcgtcgtt ttcgaggctc tgatatatcc ccgcgtccgc gctcaggcat gcaattccca
     6121 gtaacagcat caaggcagct aagaggcttc tcatcttatc cttgcgtgtc gcttgcggcg
     6181 ctcaaagaaa tcgcgcagag tggcggttac ctcatccttg gcggtgaaaa gcagatggtc
     6241 tacccgcgct ttctggagtt cctctgtcag tttgagttgc gctgcccggg tttgcttctc
     6301 gtagatatcg cgcaggacag gatcagcggt gttgacgtat tgggctgctc cggtttcggg
     6361 atcatagcag ttcaacactc ctgctctggg gagcttcagt tccgcctcat ccaatacctg
     6421 catggcgatc acgtcatgtt tgcgcgccag cattctgaga ggttcactgt agttatggtc
     6481 cagaaagtcc gagagcacaa agatcacgct gcgcttcttg agcaccaggt cggcataacg
     6541 gaaagcggcc ttgaggtcgg tgcgcttatc tttgggttcc agatagagga tgtcgcgcag
     6601 catttccagg gcacggtttc gccctttgcg ggcaggcaga tatttttcca ggatggcgga
     6661 gtacatgatc atgcccacct tgtcgttgtt tgacaaggcg gaaaaggaga gcagggccac
     6721 cagttcggcc agcttctctt tcttcagcat gatccgggtg ccaaaatcct gggacgcgga
     6781 cacgtccacg aggaagatga cattgagctc gcgggtctct tgaaagagtt ttacataggg
     6841 atagccaaag cgggcactca cgttccagtc gatgctgcga tagctgtctc cgggctggta
     6901 ttctctcact tccgcaaatt ccaggccctg acctttaaag ctggagtggt attctcccct
     6961 aaacacctcg ctcactttgg tgcgggtgtg gatctcgatc ttgcggactc gcctcaggat
     7021 gtcggccgga gtttgtgtga gcatctgccc ttatggtacc tcgatctcgt cgaagatgcg
     7081 gttgatgatc tcatcggctg taagctgttc ggcctctgcc tcataggaga ggatgatccg
     7141 gtggcgaagg acgtcgcgtc cgatggcttt gatgtcatcc gggatcacgt aaccgcgatg
     7201 ctccagatag gcatgggctt tggcggccct ggccatgctg atgctggcgc ggggcgaagc
     7261 tccatactcg atcagtccgt tcatggatga gacgcgtgga taacgttccg gatgacgggt
     7321 tgcctgcaca aggtgaagga tatagtcttt gagcttgtct tccatgtgga tatcggtgat
     7381 cacatcctgc atcactttga ctttggccgg tgtgaggacc ttgtccaccg ggatgggctg
     7441 atcgatgacc atgcggtcca taatgcggcg ttcctcttcc agactgggat acttgatgcg
     7501 caatttgaag aggaagcggt ctacctgagc ttccggcagg ggataggtgc cttcctgctc
     7561 gatggggttt tgggtggcca tcacaaaaaa cggaaaaggc agaggatagg tggtatcgcc
     7621 aatcgtgacc tggcgttcct gcatcgcctc cagaagagcg ctctgcactt tggagggggc
     7681 gcggttgatc tcgtcggcga ggatgaaact gctgaagatg ggcccccgtt tgggattgaa
     7741 ctcacccgtc cgctggtcgt agatcagggt gccggtgata tcggctggca ggaggtcagg
     7801 cgtaaactga atgcgtctga aagacgcgtc gaacacctgg gccagagtgc tcacgatcag
     7861 agttttggcc aggccgggaa ctccttccag cagcacatgg ccgtttacca gaatgccaat
     7921 caggagcctg cggaccaccg catcctgccc tactatcact tttgctattt cctcccggac
     7981 ttcatccagg atggcggaac tgtccagcac acgtctgtgg atctcttcga tcgacattga
     8041 taaccccctg tgtattacgt gccaagcttt ttggcggata atgaaaatcg ggaacagcgg
     8101 tcaaaaaagg ctattcccga tttcaaaacg gacgctttgt ttatcttatc atagcgcatc
     8161 gaataaataa gccggccggc tggcaaatgg agatattcag cgctccaccg ctttgcggac
     8221 cttgtttgcc ttgaggcagg agctgcagac tttgacccgc ttgacaccgc caggaagcag
     8281 agcgcgaatc tcgtgcaggt tcggatagaa acgacgcttg gtggcattca cagcgtggct
     8341 gcggtggttg ccaacctggg cgctctttcc acaaatatca catattcttg acatctttca
     8401 ccgtccataa ttgtttagat gatgccacaa aaatcggagc ggcttttttg acaagtcttt
     8461 atttcatcga ctgagggatc cggatgtcaa accgcgcgcc gtgaggctga acggggatgt
     8521 attccagccg gcctttgtgt tcctgcagga ttttcttgca gatggccagt cccagtccgg
     8581 tgccgtgtgc tttgccggag gtcacaaagg gcttgaaaag atcgtttctg atctcttccg
     8641 ggatgccagg tccgtcgtcg atcagactga tttgcagcca acccgcggac aagctcgcca
     8701 cgatcctgat ctcgcctttg tcggtaaggg cctcggagct gtttttcagc agatttagca
     8761 gcacccgttt aatcttgttt tcatcaaaat ggaccagttc ggacaccttg ttttcaacca
     8821 caaagttgat gtcgcggctc ttaagcgagg tgccgtaggt gtctttcagg tctttaaaat
     8881 aagcatccaa atccactttc tggatcagcg gttcgatctc ggtacccttg gcaaaatcca
     8941 ggatctctct caccatctgg tccaccagtt tggtctgttt gatgatgctg tccgtgtatt
     9001 ccgccccgtc cggatagagg ttttccagaa gctgagcggt gagcacgatc acggtgaggg
     9061 gggatttgat gtcgtggatg atcttgcttg cagccagacc gatggccatc aggcggtttt
     9121 tgctgatcat ctggttcatc agctcgataa acttatcgtt agtgttgcgc agtcgtcccg
     9181 atatagtgcg gatgagattg aacatcacca ccggatggct gaaagtgatg tttacaaaca
     9241 catctctggg aatcaccaac aactccacgt catccagtgc aaccacagtg gcggagcggg
     9301 gctcatcgtt gataatgctc atctcgccaa aaaattcacc cggatgcagg atggaaagct
     9361 gggcgaaagg ggtctcttcg ttttccaggc ctttgttgat ctcgacccgg ccgtggcaga
     9421 tgtagaagag attgtcgccc tgagtgtttt ccatgatgat ggcctcaccc tttttatgcc
     9481 aaaccctctg caggtctttg gcaaatccgt taaccacgtc ctcggaaagg ccgtgaaaaa
     9541 ttccaacctc atcgatgttt tgttgctctg tcattgttcc tcccggctca ggattgaata
     9601 gtatcacttc tccaaagaaa aaaccggggt ctagcttgtc aacgaaattc ttgctacggg
     9661 gcccattttc agcgttcagc cctgacgata ctgctgataa tcttcaactc atccgggcga
     9721 taattcagat cggtacatag cattagcgat ggctttgcgc gtgggcccgg atatattatc
     9781 tccagcacaa aacagaggag gcggcaatga ccaaagattt gaacgacatg atcacgtcgc
     9841 tacggctcga agggagctcg gaagggattg taaagagatt tgtggagcac atcaagtatc
     9901 agcgcggcaa agatcagttt tccgccacgc cccaggattg tttccaggct tttgccaccg
     9961 tgatcagaga tattatgctg gatcgctggc tggtgactca gccggaagaa tacgccaaaa
    10021 acagcaagcg ggtctattat ctttcgatgg aatacctgat cggccgcagc cttggcaacg
    10081 ccattctgaa cctggacctg ggactccaga gcctggaagc cctcaaaaag ttgggctttg
    10141 aaatgagcat gctggaggag ctggaatggg atgccgggct gggcaatggt ggcttgggac
    10201 gtctggcagc ctgttttctg gattccatgg ccaacctcag aattccggct tacggttacg
    10261 gtatccgtta cgaatacggg attttctttc agcatatcaa aaacggtgaa cagaccgaaa
    10321 ctccggacaa ctggctgcgt tatggctcgg tatgggaaat cccgcgtccg gaacggctct
    10381 ttcctgtcca ctttggcggc cggattgtga acaccgtcaa cgatcaggga ggcggacagc
    10441 ccagctgggt gccagcagaa tcggtgatgg ccatggccta tgattacctg gtgccgggct
    10501 atcaaaacgg ctatgtcaac actctgagac tgtggtcggc aaagagcacc cgtgacttta
    10561 acctgcagta tttcaacgag ggagattacg tccaggcggt ggccgacaaa aacagctccg
    10621 agatgatctc caaggtattg tatcccaacg acaacaatat ggccggcaaa gaattgcgcc
    10681 tcaagcagga atatttcttt gtgagcgcca cgctgcagga cgtgttgcgc cgctttgcca
    10741 aaaaacacaa ggattggagc gccttttccg ggcaggccgc catccagatg aacgacactc
    10801 atcctgccat tgccgtggcc gaacttatga ggctgctggt ggatgaaaag caccttaact
    10861 gggaagtcgc ctgggaaatc accaaagcca ctctggccta caccaaccac accattcttc
    10921 ccgaagccct ggaaaagtgg ccagtccagg tgctggaaag ggtcctgccc cgccatctgc
    10981 agatcatcta tgagatcaac caccgcttcc tctatgagat caggctcacc cgcaaggcag
    11041 acgatcccac cctgagacgc ctgtccattg tcgaggaaga gcccgtcaaa tccgtccgca
    11101 tggcccattt ggccatcgtg ggctcacaca gcatcaatgg agtttcagcc ctgcatactc
    11161 agatcctcaa ggataaggta ttcaaagact tctatcaata ctacccggag atgttcaata
    11221 acaagaccaa cggcatcaca cccagaagat ggttgatgct cgccaacccc ctgctggctg
    11281 atttgctgga cgacacgatc gggacagcat ggcgccagga cagtcgtaag ctatccaagc
    11341 tcaacgcttt gctggacgac agtacctttc tggatgaact ggaaaaggtc aagcaccaga
    11401 ataaactgga ctttgctgcc taccatcagg atgtctttca gcataagctc gatccggaca
    11461 gcatcttcga ttttcaggcc aagcgcttcc atgagtataa acgccagctc ctcaacgccc
    11521 tgcacatcat ccacctggtc ctcaggatca aggatggtga aacggccggc ctggtgccac
    11581 acagcttttt ctttgccgga aaggccgctc cgggatacta tatggccaag ctggtcatcc
    11641 gtctgatctg cgccatcggg acttacatcg atcgcgacag ggacctctcc aaatatctca
    11701 aggtagtgtt tttgcacaac tatcgcgtca ctctggcaga aaggatcatg ccggccgccg
    11761 aagtctcgca gcagatttcc accgcgggaa cggaagcctc aggaacgggc aacatgaagt
    11821 tcgccatcaa tggcgcactc acggttggca ctctggatgg ggccaatatc gaaatcaggg
    11881 acgcggtggg ccccgacaac tttttcctct ttggcatgac ggcagagcag gtgaaacagc
    11941 tcaaacagac cggctaccac ccctacggga ttgcccatgg cgatccaacc atcaaacgga
    12001 tcatcgattt catcggttcc ggagaactgg acgcgttcca tcccggattg tttcagccaa
    12061 tcctgaacag ccttctgtat cagggggatg agtattgcgt gattgccgat ctggcggatt
    12121 atacccgcgt catggaagca gtcaacgacg cctatcctca acgtcgacag tggaatagaa
    12181 tgtcggctgc caacatcgcc aacatgggcc ttttttcttc cgatgtgacc atcgaggcct
    12241 acgccagaga aatctggggc gtgatcaaat agaccgcgcg gcggcttaca cagcgcttcg
    12301 cgtccgcatt cttgctcatt ggttgccgtg gagatgccgt gcgccctccg gcgaaaaacg
    12361 ggggagaggg cacggtatct ccacggcaag tgattgggca ggaaagcggg aagcttgtgt
    12421 tgccaaagat cactggacgt cctccacgct gtcggggatc gcggtcgggg cagcagaaaa
    12481 gcgcgtcagg agtttattga tgacaaaccg agatgaagtg cttgcgtacg caggcagatg
    12541 cattagatta tgagacatgg ctgtctggcc atggggagct ttgtgcccca tcaatcatgc
    12601 cagagggtca aggagattgt atgataaacg ccaattccgc ggtagtccgg ctgcgttttg
    12661 cccttttact tattctgatc attgggattg gagcttccgc tctgcaagcc caggtgctgt
    12721 ttagcttcaa tgtcaacgat ttggaggcct acgaccgtag cggtaactgg atttatgatg
    12781 ccgatggacc tggctttcca aaccacgacg cgcaatggtt cacgtttagc ccagtggtgg
    12841 gatatccctt acattatttt gccgaaaggt atatccatga aggggttacc accaccctca
    12901 cctgcttggc ggagaacgta gctcaagcag ataatcctgg ccagctcacc atgagcttca
    12961 cccagtttaa cctcgtgtct ttcaaacgga tcaataccgt caacccgctt gctccatgga
    13021 acattccagg ccaatcaggt gatgaacggg tctatgccaa tgccggtgga acgatcagct
    13081 ataatggcac acctgtattg actatgacgg acgccacctt cgtgatcacg actccctatc
    13141 ccaatcaggc ccaaattcaa gctctcagtc ccatgctggc tacttggaca ggagacatcg
    13201 ggaccggcgc tcctcagacc ggttttggct atggcgatct ggacctggca cattccgatc
    13261 cggcctggat tccgctcttc gctgcttcca actataagat agaattgcag atggcgggga
    13321 ttacatcggt ggtcactccc acagcgggat atttcgattt caccctcaat gtgctgcctg
    13381 cgaccacttc ctacattggt ggcaacgtag tcgtggacct tggcaacctg ccggagctgc
    13441 atcccatccc tggtacggat gccaccctgg gagccattgc tggcaccccg ggaggccaaa
    13501 atggcgatct gcgcacccta ttcctggctg aaatcggtgt ccctcccagt ggaaacatcc
    13561 ccgctggttt caattacagc accaagaagc attgggaact gggcggcact cttcagacct
    13621 ttaacgtgaa cgtgtcactg aacctcggtc tggatgattt tcaggccaaa gctccctcgg
    13681 actggaggat cgtatatagg tccaatgcct ctcaggactg ggcgttatgg ccagatcaga
    13741 tgctctcggg gcctactacg atcacagctc aaaacgtgac ggattttgcc tatttcgcgg
    13801 tggtttctcc ctttgacgag accctgcctg tagtcctcag ctcctttgcc gcttatgtga
    13861 actccaactc cctggcggaa ttgaagtgga ccacggcctc cgagacggat atgacgggct
    13921 tcaaagtcta ttccagttct aatacccagt tgggctccgc catttgcctg accccggtcg
    13981 tgattccagc ctccaacacc tccgacggcg ctcactattc atacaccgcc accgagcttg
    14041 aaggtcccgg caccttcttc ttctggctgg aagcgctttc catgaacggt cagagcgatt
    14101 tctacggtcc cgtgtccgtg aatttgccag ccgatcctgg cactcctgtg ctgccacccc
    14161 gcagcgaact cggcattgct tatcccaatc ccttcaggag cgggtccatg acccggatcg
    14221 cggtggatat caaggaaggc gagactggca ctctggaaat ctataacatt gccggacaga
    14281 agattaagag ccagactatg gcggccggca gccacaatat cgactgggac ggcaaagatg
    14341 ccagcggtcg tcaggtggcc agtggtgtgt attattacaa gctcagcacc ggctcctaca
    14401 cccaatcccg caagctggtg atcgttaagt aaagcatccg cttcataaac attcagcccg
    14461 gtaaggatat ccctgccggg ctgatgcttt tatggcgttg ttgtctatat ctttacactt
    14521 acgttgatgt tcctgaatct ggccggggcc actccatgtg agagtagcat cgcctggccg
    14581 ggttcgcctt ttccacagtg gaaggtgccg tggaacttcc attcctgctc tccgcagacc
    14641 ttatcgcagg cgttccagaa gtctttggtg atgccgaaat aggtgggctc cttcacgatt
    14701 ccggtgatct taccaccgtc gatgcgatag ccaatttcgg tggtgaactg gaagttgttg
    14761 cggttgtcgt cgatgctcca ggtcttggtg aaatccagca ggtagccgtg ttcggtggat
    14821 tcgatcagtt cattgagact cccgcttccc ggggccagac agaagttggt catcctcacc
    14881 agcggaaagt cgtccgcgaa gctggctttc atgtttgatg agggttccag gcccaagagg
    14941 tgagcaatgt ggcgggaggt ttgctggtct ttgaggatgc cgtctttgat gatgtcgacg
    15001 cggcgtcccg gaactccttc atcatccatg gggtggtaac ccatcccacg gggatcactg
    15061 ctatccgaat agatgttaac cgcttcggag ccatattgat agctacccag catggctggt
    15121 ttgacgaagg tcttgccggc ataggatatc tccatgccaa agatgcggtc cgcttcggtg
    15181 gcgtggccta ccgattcatg gagttgcaag gccagatggc ctcccccgat gataatatcg
    15241 gcccgttctt cttcgatgcc gggagcgtct aaaagctcgg tggcttcctt gatgatccgc
    15301 tccgtattct cttcaaacat ctgctggtaa aagagttcga agcctccgcg tcccgcactc
    15361 atgtggccag gccaggtgcg cgtctgcatc tgtttgccgt ccgcggcgat aacttccata
    15421 tagggcaggg tgttatagac ggtggtgtgg ctgtagctac cctcagaatt ggcgtaatac
    15481 ttttcctgac gctgaaactg agcggctaca aaggagtgga cgatcttgcc ctgaggcttg
    15541 atggcagagg ccaggcggcc caggtattcc agcttctcgg ccctgtccat caggaaggga
    15601 tcgatctcgg gctcaaaatg gtagtccgcg atcgtggctg gcagcatggg aaagcggacg
    15661 gggtctttga taaaaccact gccgcttttg gcattggtga tggcagtctt gatgacgcgg
    15721 tcgatcgagg ctttggagag atctccgctg ccggcaaatc cccagcagcc gttgatcagc
    15781 actcgcactc ccagggctgg ggaggctttg ctgctgccat aatccctcaa aaagccgttc
    15841 atgaaacgga ttccttccga gtctgtataa gtgatccggg catccgcgaa ggtgacgcct
    15901 ggcaggttga gcttggtgat gatgtctttg atcagatgtt ccatgagagc tcctatatgg
    15961 tgtgggtgga agagctgatg ttgaagtcct tcaccaacgc gtgaggtgct ttgacgctct
    16021 cgatgtcaaa ttcctcatag ttttcggaga aggggatggt gtagccgcgg tcctcgaggg
    16081 cctgcacgtg gttgaaaatg cgttcgatgc gttctgtgaa gcggagggaa tttaccaccc
    16141 gggtgatctt gccatcctcg atcaggaagg tgccgtctct ggtcagaccg gtgagggagg
    16201 tttccttcat attgatgaaa ttcatgtaat gcagactgga gatatagagg ccacgtctga
    16261 cactgccgat catctcatcc agagtcttgt cgccagtagc gatactgagg caggaagcgg
    16321 tgttaccgtt tttgggcagg ccggtcttgt gggcatagta gttgttgcac aaaaaggctt
    16381 tgaacacgcc ccggtcgatc agcttgaggg gattgtagat atggccgttg gagccgtagt
    16441 cctgaccgat caggtccggg tccctgggat cgtccgtgat gctgatccat tccgggaaaa
    16501 cttgcttgtc cagcttgtcc tcgaagaagc tgctgtgctc gtcaaaggcc tgcgcgctca
    16561 tgccccagca gaggtactga gcgtattccg ctacacagcg gggtgccagg atcacatcgt
    16621 aatatccagg ctccacatcc accacgggat tggcgcagta ggcgatcttt tgcaggagcc
    16681 gtgctttgaa atcctgggga tcaaaacgat tgaagtcatc accaccaaat gtttccagca
    16741 cagtgatttg ggactggttg tgcacggcct tcacctccag gtagatgggt gaagagctgc
    16801 tggatttgtc caggccgttg ctgttgacga tgcggtgttg gacgtagttg cagataaagg
    16861 tgccataaat ctcaaagtca aagggtttgg cggcttcggc cagttctttg aggatggcgg
    16921 tcttggtgtc caaagggatc agctcgatgt tgttgttcaa ctggcgcgga ggtgcgatgc
    16981 tgagatcggt ctcaagatcc acaaagtcag gatcttcagg cagttggtcg atgatagcga
    17041 gggcgtccct gagggccaga tcgatcttat cctgattggg atcgtcgatg ccaaagcgat
    17101 agcttttttt ccctcgccac aagcccacgc tcaggctgag cttgtcttta ctgatgttgt
    17161 agttggtctg gctgtgaaag aagcgcagaa agtctgtgct ccaattctgg ctgctgagct
    17221 catatttgag cccactttgg cctgcgaggg cactagccag atagtttaga tagtccattt
    17281 atcctccagg agttgtatgg ttttatagtt tta
//
LOCUS       DAOSFN010000042        17265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2684_length_17265_cov_9.422022, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000042 DAOSFN010000000
VERSION     DAOSFN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2684_length_17265_cov_9.422022"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PLO35_04260"
     CDS             <1..1001
                     /locus_tag="PLO35_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78911.1"
                     /translation="GEYNVQNLGFNYTAIEQGGPHGALNDLVVLEQGHEILMGVPEEV
                     DEVHPKAWLLATTLESFTTDISFDLSGSGFGTSDQWVVLRKPENAQNWQIWPNVSYLS
                     EDVIRANNVTAFSEWAIGKLGDDTLPVQLSSFTGTATSQGSVVLNWTTQSESNLIGFR
                     IFRAETPYLDNSLMVGDLICASNSSNLTHYSFQDTESLPEHWYHYWLQVLDMDGNGSF
                     HGPVTVLCTQLDTDVEIPPLDQITGIQSIYPNPGHHQTIKIYLERDQGISMDIYNLKG
                     QKVKTIYRGGISMGQHNLDWDGTSDLGLDLPSGVYFVTFSSSSGKTLTKRKISLVK"
     gene            complement(1172..>1744)
                     /locus_tag="PLO35_04265"
     CDS             complement(1172..>1744)
                     /locus_tag="PLO35_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78912.1"
                     /translation="LFVLAACSNEPVMSAPEAPAQAELYRIEETTGPGRGISYPVSFD
                     SEARNQAGYMMISNDAQNLYLGYFMNDNWRLNKSRVFIGRSMEDIPLDDAGRPVVERF
                     TYGFELDRGMSSYTYRIPLAELKVRPGQFIAIGSYATVQQLDGDFSSPSSPGQDSSWW
                     FSSGYRLSRPVGVVITDDYEEIITRSNLEF"
     assembly_gap    1745..1844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1845..3304)
                     /locus_tag="PLO35_04270"
     CDS             complement(<1845..3304)
                     /locus_tag="PLO35_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOH78913.1"
                     /translation="MKNVSIAPDGKEYVMPTAEESAAERELLIKITEEQRKLGRQIVA
                     VQGLGFVGCVMATVVADATDKDGKPYYYVHGHQRASKRSYWKVPVINSGIPPVASSDP
                     EVPQIFHRTVVEKQNFRGTSEDFVYSLVDVVVVDIQLDATKPAFGEAEKGYCDITHFR
                     EGIRTLGQNIQPHCLVLVETTVPPGTCEKVVKPILEEEFTKRGIDIRINPPLVAHSYE
                     RVMPGSKYVASIRDFWRVFSGVNQKSIELCREFLSNCLDVDNYPLTQLESTNASELSK
                     TMENSYRATNIALTLEWARFAEQIGVDIFKVRDAIRKRKGTHDNLLRPSLGVGGYCLT
                     KDPVLANWAMQTLFGLEGSLDVAIRSVNINDTMPLHTVRLIEREYQTLRNIKVAVLGV
                     SYLENVGDTRHSPSKTFVEALRKDFAIVRTHDPYVEAWPELEDSCVESDLMAVLPEAD
                     VVVFAVGHDQYMKLKPEELIAMCGTKPLIVDCSNFLS"
     gene            3762..5189
                     /gene="pyk"
                     /locus_tag="PLO35_04275"
     CDS             3762..5189
                     /gene="pyk"
                     /locus_tag="PLO35_04275"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOH78914.1"
                     /translation="MNFRSHKTKIVCTIGPAIDSPEMMRQMIAAGMDIARLNYSHGDF
                     SYHAANILKLRKAAHEEGRRLTIMADLPGPKMRIGKLDRESYILRTGDELILTTESII
                     GNYQRVSVSFEPLPRVVKPGDKISLNDGIIQVEVAKVLGNDVVCLIKVGGEIRSNKGL
                     NILDIDLGIDVFTSHDRECLKSALENGVDAVSQSFVNRAQDILALREEAARLGYNPFV
                     IAKIERGEAVRGIDAICAVSDAIMIARGDLGVETDISRIALVQKHLTARANYFGVPVI
                     TATQMLESMVSNPLPTRAEVTDVANAILDGTDCVMLSEESAVGKNPVSAVQMLARIAE
                     HTEAQRNDPEIGKPRKDFFRGDADHISDLITHNVSISVEKLHPAMVVANTVTGHTARM
                     IARFKLPVWMLAVASEHSVCQNLQFSSGVFPYQVDDEPQDWDAFIRSVTCDMQLSSHL
                     LILVKGPSNLNPIANHLIEIIDLSN"
     gene            complement(5695..6273)
                     /locus_tag="PLO35_04280"
     CDS             complement(5695..6273)
                     /locus_tag="PLO35_04280"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOH78915.1"
                     /translation="MKARIAICMIAAMMFGVLYAADEELVPVVDFSISETPREAKMEE
                     MTVAGFQVMNTMEHDAMMKVWGDFMSVVGKLPCDPSAPYYGVNFFTKDYNPQDHTGFG
                     YMACAPVLSTEKLPEGVVARKIPARDYIVFEHKGPLKYLEESFNYIFYKYLPQSDYKA
                     LFTDVLEVYDCRFDAESLDSIIEIWMPVEPKE"
     gene            complement(6358..6489)
                     /locus_tag="PLO35_04285"
     CDS             complement(6358..6489)
                     /locus_tag="PLO35_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78916.1"
                     /translation="MWHAPLPDGLFMLLGETDLNQYIHSGGAVAPRAFYRVLTIDSE"
     gene            complement(6575..7954)
                     /locus_tag="PLO35_04290"
     CDS             complement(6575..7954)
                     /locus_tag="PLO35_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOH78917.1"
                     /translation="MKKEKLYVLEQMPITRSIIHLALPSVLSMLVNILYNLTDTFFIG
                     KLNDPYQVAAVSISLPLFTFQMAIAGIFGNGGGSYLSRLLGKKQYQEAHETATTAIFS
                     SAASSILLAVLGILSIPLFLKAVGASGMVKAPAYDYMFIILLGTPFIMLKFTLVQLLR
                     AEGAAKAAMAGLFIGTGVNIILDPLFIFVFKMGVTGAAVATVIGQGLGASFYLYYYLS
                     GRSLASPGLKYLCFKLETYRQILYIGIPASLSQIMMSIGNTISYKLAADYGDHAVAAL
                     GVAARVFSIPIFVFIGISVGVQALIGYNYGARNYPRMKKAIRTSITISLWLGAFFTLM
                     FALFPAQLITAFIKDVKIMELGTVILEAYVFAIPMAAIGMILMASLQAMGKALPALIV
                     ALSRQGIAYIPLIYLLTRLYGFEGLVFALPLADYLTTAVSFGFVWFILRGLKTQGLGP
                     VAERMPETL"
     gene            8135..10588
                     /locus_tag="PLO35_04295"
     CDS             8135..10588
                     /locus_tag="PLO35_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH78918.1"
                     /translation="MRRVLMSMLMLFMAGWLLADQNDPPAKGRFEFFPQSPYRVSKLE
                     QQAKGADRTTIWSTNFSSSTGWDLAYAWNMGTPTMGPDTVPDGNGCLATNLAGFYAND
                     SNIRALSPSIPIPAASYVELSFTEWFQLESDYDFGWVEIKQGSTYYSVDARSGTSGGQ
                     WRETSIDVTRWQGSSIQVVFHLTSDASVPALGWYLDCAAIDIVEPHPLELDITGINIS
                     NLPSVYLTASVRSPDGPVTTLTQSNFSVWENSSAQTNLFSVIAPDNSQTASSTDIVFV
                     LDVTGSMGDEIASVRANMQAFMNHLQGQNMDYRIGFVVFGDIVYVYNQYFFYTSFTEI
                     MNIISNIELGEHNIGSGGDGPENQLEAMAEGSVFSWRPGASRVMIMLTDATAHEADYV
                     TDWTVNDLLAQRLLPNEVMVFPIFNTNNSGQLAQYLPIAQQTNPSAACYYIYDNFNDI
                     ISEIGNYITSLYTVHYISPVQADDPMSRIVKLTASSGADSSEDYAFYLPGISPIITRE
                     AELEALDYTPVPAVWPLELEVVIEDRVPPGLLTQSIIWRHVGTSAWYSAPMTMIKEDY
                     YQAILPANQVSGAGIEYYISASDGQTSVTLPSSEPELHPFSIAVSPAGHASFGTPTAS
                     YSSSSQLAVSVTATAFTTPQLKLYYRPMGALIYEEAAMAQSGNNFSATINQNLGSLGV
                     QYYILCTQANQLITYLGNPDEPLYVTAGALAPQVSDAESLIFDSRAWPNPVSLSQRAG
                     YVSIGFSLKEETSVQARIYNLRGQLVKELSDKHLVPGQHTLWWDLKDKEGARVSSGVY
                     LYRLQADGYAASGKLLIAQ"
     gene            10599..12665
                     /locus_tag="PLO35_04300"
     CDS             10599..12665
                     /locus_tag="PLO35_04300"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH78919.1"
                     /translation="MKKLSIFLLLVLLAYSLFANGVLIKNGISGQYLTITGCRVETVI
                     TNQVATTVATMRFYNNYSVSTSTVFAFPHPPEASPTALSWFVNGAWHDAVIEPGAGGS
                     VVPPGEDMYPALDNYLGKTYLAFPVSEHVQPAAEMIFRVTYVQLLPYLNGRVEYDFNS
                     SYGYLTNQPLDSLSVDIQISGNRTISNVLLPGFTNVNIDIDGPTASVDLMASTFNLDL
                     DIELSYDLAMENLGASTFSTFIDHQYVPDDLGDGFFMSIVEPQPSADVIQKYFTFMLD
                     RSGMMNDASMQQAKDAASYMIQNLNAGDYFNIVDFATVAGTFAVNHVPFNPANRDLAL
                     SYIANLEPEGSCNISGAFDTAIPQFGGAPDEAAHVIVFLTIGHPSTGIIDTPTLVDHI
                     DNLVTATDRDVNIFCFGVGTNVAFQLLSMISAAHQGIATYVGLNELLDVLIDFYTKIR
                     NPILLDPELEVVGQTGNITQIYPDPLPNLYLGNQMILCGRYHQAANLVFNIGGYVLGS
                     HVNYHYEHALGQTSEPGMQFLMKIWAKLKIEHLMALYYQLDPYSPQAIALHDQIVQIS
                     IDYGVLCSFTSFSGGEVANPEDTVPPALHPIRITACGPNPFKNHVDLKVMVNDAKRGP
                     LTLNIYNARGQLIRSIIIYPDKAGEYIFRWDGRDAAGRSVASGVYLCRIGVTGFSQTA
                     RVVLIK"
     gene            complement(12877..16728)
                     /locus_tag="PLO35_04305"
     CDS             complement(12877..16728)
                     /locus_tag="PLO35_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78920.1"
                     /translation="MRRILTLAAVLLWVGLSALSTEVGSIRGFLYGSEPACAYDNWVS
                     HVAEGKVSWLNVYAPWDEQNNDFGDYHIPSTEELNQWAQVVQAWLALDLEGAQAVIDS
                     CGFPYDVVQFQDTDSGRLFYMLREQINAQLDTNDSEDPELYEYGSFDYGWGLYIYDPS
                     ASRPMMITAPHPCDDYPSPVISIEALLAWDARFLLVAGAGREVAYFPPYHSNNQSISD
                     PSRFADHPFNVTYQHAATQLRGLTGRTEFSVQLHTYDWNKYPNKAPVMLSAGNGRLHP
                     TLPVRDHRRAPNDLIHNTPWQVMEANSFGTHDAVDVSQYYSIYRASAYEIYYEQDGIS
                     AFIPQNTDLPGASENCQMLFTQQFNLYDSYSPFLHIEMDELPRCFDQSGDQRDLFYGY
                     SEEMGWPLATRYTRFLGFYWPVIEALDLCLDELLMMNDGNAPSNPENFRISGVSASAY
                     VLTWQRSYDYDFDTYEIQTRWHDGVSWQLSESDRNLYEDLAWQPLNEYMLSFPENSTM
                     IYVRMRARDKHGNVSAWTEELKVFRPASGMGSFAQFALQPDNGRIKVSFQANITAAVG
                     YNVNRGTGSGPISAYATAEEHPELQHNQQGVYEFWDNAATNGQVYRYQLSAVYADGSE
                     YWSPQILSAHAFKPITLYFRDLGNGQADSLKIAVNAFATDAQDALDIEKPGPGFSTVW
                     IASTLANPAATLARDVKAPYYPHSDSKPWTVKVFNSLAGQQLRLENDFNVTPQEGDLL
                     IKDNYSGLWHDFRTGPLTWTATSSGYRTFELWWGYKAPWIEFPALGLQRFEAGTPVTL
                     NWQVVNPARVTSVELWMVGPEDSILVCTGLSPTQTAFVWSGSGLPMIGSRLVVKGLAT
                     DGSELRGESPYLYDLLPHNMVYNGAAGHSLICIPIAGWSAGVDELFSAGTTVWELDSV
                     ANWQNVDTLTGNHSYLVWGPQAWQLSLPMTENLGLAYQNLNHGWNLVANPHYHRYALG
                     QLSFDLAGASYSWDELVAQEIISPRLYAFSETGWALTDHIEPLSAVLMWYQGSQPLNL
                     IFNPQVSGTAVNSWPLLWSLSLSAWDGFRGRDGVEIGVADDASETWDPQFDLSKALLM
                     PAQELSLALGTVATGPLQSEIKGLYPDNDQVTKTWTFNLEVANSGPVAFSYCSSDLPA
                     SYEVELYLDGQIRLLTPATYSWIDVSAGTHEGQIRVRNYQSASLYAARLPELKAWPNP
                     FRGSVALSVTSGKADFMKAEVYNLRGQKVRGLKAIPDGDKLTFSWDGKDSSGHSTASG
                     IYFIRVGHGAKAKTVRVLKLD"
BASE COUNT         3912 a         4804 c         4585 g         3864 t
ORIGIN      
        1 cgggtgaata caatgtgcaa aacctgggct tcaactacac ggcgatcgag cagggcgggc
       61 cccacggggc tttgaacgat ctcgtggtgc tggaacaagg ccatgagatc ctgatggggg
      121 tgcctgagga agtggacgaa gtgcacccca aagcctggct gctggctacc actctggaat
      181 ccttcaccac cgatatcagc tttgacctca gcggctcagg ttttggcacc tcggatcaat
      241 gggtagtgct cagaaaaccg gaaaacgctc aaaactggca aatctggccc aacgtgagct
      301 acctcagcga ggatgtcatc cgggccaaca acgtcaccgc tttcagcgaa tgggcgatcg
      361 gcaagctggg agacgatacc ctcccggtgc agctttcgtc cttcaccggc acggccactt
      421 cccagggatc ggtagtgctg aactggacca cccagagcga atcaaacctg attggcttca
      481 gaatcttccg agccgaaacc ccctatcttg acaacagctt gatggtaggc gacctcatct
      541 gtgcctccaa cagctccaac ctcacccatt acagctttca ggatactgaa tccctgccag
      601 agcactggta tcattactgg ctacaggtcc tggatatgga tggcaacggc tccttccacg
      661 gccccgtcac agtcttatgc actcagctcg atacggatgt cgagatcccg ccactggacc
      721 aaatcaccgg tatccaaagc atctatccca atcccggcca ccatcagacc atcaaaatct
      781 acctggaaag agatcagggc atcagcatgg atatctacaa cctcaagggc caaaaggtga
      841 aaaccatcta ccgcggcggc atcagtatgg gccagcacaa cctcgactgg gacggcacca
      901 gcgatctggg actggacctg ccctccggag tttactttgt gactttctct tcttcgtctg
      961 gcaaaaccct gaccaaacgc aaaatctccc tggttaaata accctctctc cagactcccc
     1021 cataaacagg cccgtcgcca ggcgggcctt tattttgcgc gggaatttat ggcagaactg
     1081 atctgtgtga tgatgaaaaa gccggaagct ttggaactcc cggcttatct caagggagga
     1141 tggtcctccc gtttaaagct gtgaaaggtg cttagaactc gagatttgac ctggtgatga
     1201 tctcctcata gtcatcggtg attacaacac ccacaggacg gctcagacga tagccactgc
     1261 tgaaccacca gctgctatcc tggccagggg agctcgggct ggagaagtct ccatctaact
     1321 gctggacagt ggcataggag ccaatggcga taaactgccc gggtcttact ttgagctcag
     1381 ccagcgggat tctgtaagtg taggagctca tccccctatc cagttcaaat ccataggtga
     1441 agcgctccac tacaggcctg ccggcatcgt ccagagggat gtcctccatg ctcctgccga
     1501 tgaaaaccct gctcttattc aagcgccagt tatcattcat gaaatagccc agatagaggt
     1561 tttgagcgtc gttgctgatc atcatatatc cggcctggtt tctggcttcg ctgtcaaagc
     1621 ttactggata actgatcccg cggccgggac cggtggtttc ttctattctg taaagctcag
     1681 cctgggccgg agcctcagga gctgacatca caggctcgtt ggaacaagct gccaggacaa
     1741 acagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngagagg aagtttgagc
     1861 aatccacgat gaggggtttg gtgccgcaca tggcgatcaa ttcttccggt ttgagcttca
     1921 tatactggtc atggccaacg gcgaagacca ccacatcggc ttccggcagc acggccatga
     1981 ggtcgctttc cacgcaggaa tcttccagtt ccggccaggc ttccacatag ggatcatggg
     2041 tgcggacaat ggcaaaatcc ttgcgcaggg cttccacaaa ggtcttggaa ggagagtgac
     2101 gggtgtcgcc cacgttttcc agatagctca ctcccagcac ggccactttg atgttgcgga
     2161 gggtctgata ttcgcgctcg atcagcctga cggtgtgcag gggcatggtg tcgttgatat
     2221 tgacggagcg gatggccacg tcgaggctgc cttccaggcc aaagagggtc tgcatggccc
     2281 agtttgccag cacgggatcc ttggtgaggc aataaccgcc cactcccagg ctgggacgca
     2341 agaggttgtc atgcgtgcct ttacgtttgc ggatggcgtc gcgcaccttg aagatgtcca
     2401 cgccgatctg ttcggcgaag cgggcccatt ccaaagtgag agcgatattg gtggcccgat
     2461 aggagttttc catggtcttg gaaagctcgg aggcgttggt gctttcaagc tgggtgaggg
     2521 gatagttgtc cacgtccagg cagttggaga ggaactcacg gcagagctcg atgctctttt
     2581 ggttcacgcc ggaaaagact ctccaaaaat cacggatgga ggccacgtat ttgctgcccg
     2641 gcatcacacg ttcataggaa tgggccacca aaggaggatt gatcctgata tcgatgccgc
     2701 gcttggtaaa ttcttcttcc agaatgggtt tcaccacttt ctcgcaggtt ccgggcggga
     2761 cggttgtctc caccagcacc agacagtgag gctggatgtt ctggcccaga gtgcggatgc
     2821 cttcccggaa atgggtgatg tcgcaatagc ctttttcggc ttcaccaaaa gcaggcttgg
     2881 tggcatcgag ctggatatcc accaccacca cgtccaccag gctatagaca aagtcttcgc
     2941 ttgtgccgcg gaagttctgc ttttccacca cggtgcggtg aaagatctgg ggcacttctg
     3001 gatcggaaga tgccaccggt ggaatgccac tgttgatgac cggcactttc caataggacc
     3061 gcttggaggc acgttgatgc ccgtgcacgt agtagtaagg cttgccgtct ttatcggtgg
     3121 cgtccgccac cacggtggcc atcacgcagc ccacaaagcc caaaccctgc acggccacga
     3181 tctggcggcc cagtttacgt tgttcttccg ttatcttgat cagtagttca cgttccgccg
     3241 cgctttcttc cgcggtcggc attacatact ctttcccatc cggggcgatc gacacattct
     3301 tcataaacca atgactcctt ggtgctgttt ttcactattt cgtgcaaaaa tcggaaaggc
     3361 tgatgttgtc aagcggaatc tcaagtgcgg cttatctaca cgtattcagt gtatatctat
     3421 acatcttgtt atccctcact attaatgcag taatccatag cttggacaag gtatttatgc
     3481 tataaggtgg tgtatggaga accatgtcct tctatcatca tcaggcttat tctaatgcta
     3541 atcatgaata gctatttcga gtatagcagg ctcagacata tttgaaatga cgtttagcag
     3601 ttttggatgc tatcagcttg ttttggaatg tgttacaatg gcaatggaca aatctgggag
     3661 tcatatccct gatttaacag gattacttga caatatacgg ggtgaacaga taattggcca
     3721 tcatgacagg gcgtaaaatc ccgcgcccaa aggagcccac catgaatttc cgctcccata
     3781 aaaccaagat agtctgcacg ataggccccg ccatcgactc tcctgagatg atgcgccaga
     3841 tgatcgcggc cgggatggat attgcccgcc tcaattattc tcatggggat ttctcctatc
     3901 acgccgccaa catcctcaag ctccgcaaag ccgctcatga agagggcaga aggcttacca
     3961 tcatggcaga ccttcccggc ccgaaaatgc ggataggcaa actggaccgc gaaagctata
     4021 ttcttaggac aggcgatgaa ctcatcctca ccaccgagtc catcatcgga aactaccaac
     4081 gcgtgtccgt gagttttgag cccttgcccc gtgttgtgaa acccggcgac aagatatctt
     4141 tgaacgacgg gatcatccag gttgaggtgg ccaaggtgct cggaaacgat gttgtctgcc
     4201 taatcaaagt cggtggcgag attcgctcca acaagggcct gaatatactc gacatcgatc
     4261 tgggcataga cgtcttcacc tcccacgacc gcgaatgcct caaatccgct ttagaaaacg
     4321 gagtcgatgc cgtcagccag tcttttgtca accgggccca ggacattctc gccctgcgtg
     4381 aggaagctgc caggctgggc tacaacccct tcgtgatcgc caagatagag cgtggcgaag
     4441 cggtccgggg gatcgatgcg atttgcgcgg tttcggatgc catcatgatc gccagaggcg
     4501 atctgggagt cgagaccgat atttcccgca tcgcgttggt ccagaaacat cttaccgccc
     4561 gggccaatta ctttggcgtt cccgtgatca cagccaccca gatgctggaa tccatggtct
     4621 caaatcctct tcccaccagg gctgaggtca cggatgtggc caatgccatt ctggatggca
     4681 ccgattgcgt gatgctgtct gaagaatccg cggtggggaa aaatcccgtc agcgcggtcc
     4741 agatgctggc caggatcgct gaacatactg aagcgcaacg caatgacccc gaaatcggca
     4801 agccccgcaa ggattttttc cggggcgacg cggaccatat atccgatctg atcacccaca
     4861 acgtttccat tagcgtggaa aaactacatc ctgccatggt ggtggccaat acggttaccg
     4921 gccacacggc caggatgata gccaggttca agctgccggt gtggatgctg gctgtggcct
     4981 ctgagcattc tgtgtgccag aatctgcagt tctccagcgg ggtttttccc tatcaggtgg
     5041 atgatgaacc ccaggattgg gatgccttta tcaggtccgt tacctgcgac atgcagctat
     5101 cttcccacct tctgatcctg gtgaaaggcc cctcaaacct caatccaatt gccaatcacc
     5161 tcattgagat catcgatctg agcaactgaa cggcatcgga tcacactcaa acgtcgcatc
     5221 cgactgattg acaagtgatt gcaccactcc gtcaactttg cgcgacgatc cctatctgat
     5281 tagatctcag acttttccct gtccttgcct ggtcacccgc ccttgagatg ccgtgggctc
     5341 tccgccgaaa atcgcgggag ggcgcacggc atctcaaggg cggctaaaga gcaaggatga
     5401 agacccagta gttgagcttc cgtttttgat ttgcctcttc gcccttactc gtggttcgct
     5461 tgcctgtccg tacctccccc aaggcatccc caatcaagtt cgggatgggt aggacatggt
     5521 agtgacagac acagggccca tggataaggg atgatgctgg aatagtaaga aagagagcct
     5581 catattctca tatctcatgg taaaatgttc tcatattctc tccatttctc tatgtaaaca
     5641 acaggccccg aagcatactc cggggcctga aacgcggtta gaaaagcggg tggattattc
     5701 tttgggctcg acgggcatcc agatttcgat gatggaatca agagattcgg catcaaagcg
     5761 gcaatcatag acttccagca cgtctgtgaa gagggccttg taatcactct ggggcagata
     5821 tttgtagaat atgtagttaa agctctcttc gagatacttg agagggcctt tgtgctcaaa
     5881 gacgatataa tcccgggcgg gaatcttgcg ggccaccact ccctcgggga gcttttcggt
     5941 gctgaggacc ggcgcgcagg ccatatagcc gaagcctgtg tggtcctggg gattatagtc
     6001 tttggtaaag aaattgacgc cataataggg agcggaagga tcacagggga gctttcccac
     6061 cacgctcata aaatcgcccc acaccttcat catggcatcg tgttccatgg tgttcatcac
     6121 ctgaaatccg gccaccgtca tttcttccat cttagcttcc cgaggggttt cggaaatgct
     6181 gaaatcaact acgggaacca gctcttcatc agcggcgtaa agcacgccaa acatcatcgc
     6241 ggcgatcatg cagatcgcga tcctggcttt catcttggcc tccttggcta atatttgatt
     6301 ccatccctgg agtcttatca tatttgtcaa gcacaatagg cttccaaaac ggaagggcta
     6361 ttcgctgtcg atcgtgagca ctctgtagaa cgctctggga gcgacagccc ctccggagtg
     6421 gatgtactgg ttgaggtctg tctctcccaa cagcataaac aagccatcgg gaagcggcgc
     6481 atgccacact ttgtatccat ccgccccggg gaccttatcc cactccagaa tgagatcggg
     6541 gtccgcgcgg ctcagttatc tgtaattgat tctatcagag ggtttccggc atccgctctg
     6601 ccacaggccc cagcccttgg gtttttagcc cgcgaaggat gaaccacaca aagccaaaag
     6661 acaccgcggt ggtgagatag tctgccagcg gaagcgcgaa cacgaggcct tcaaagccat
     6721 agaggcgggt aagcaaatag atgagcggga tataggcgat gccctgtctg gacagcgcta
     6781 cgatcagagc cggcagcgct ttgcccatag cctgaaggct ggccatcagg atcattccga
     6841 tggccgccat ggggatggca aaaacatagg cttccaggat gacggtaccg agttccatga
     6901 tcttgacgtc tttgatgaag gcggtgatca actgggcggg gaagagggca aacatcagcg
     6961 tgaagaaggc tcccagccac agggagatcg tgatcgaggt gcggatggcc tttttcatcc
     7021 tgggataatt gcgcgcgcca tagttgtaac cgatcagggc ctgtaccccc accgagatgc
     7081 cgataaacac aaagatgggg atgctgaaga ccctggcggc cacccccaaa gcggccacgg
     7141 cgtgatcgcc ataatcggct gccagcttgt aggatatggt gtttccgatg ctcatcatga
     7201 tctggctcag tgaggctgga atgccgatat agaggatctg gcgataggtt tccagcttga
     7261 aacacagata cttgagccca ggcgaagcga ggctgcgtcc gcttaagtag taatatagat
     7321 aaaaggaagc tcccagtccc tgaccgatca cggtggcgac ggcggccccg gtgactccca
     7381 tcttgaagac aaagataaac agcggatcga gaatgatatt gaccccagtc ccgataaaaa
     7441 ggcccgccat ggccgctttg gcagcccctt cagccctcag aagctgcacc agagtgaact
     7501 tgagcatgat gaaaggggtg cccagcagga tgataaacat atagtcgtaa gcgggtgctt
     7561 tcaccattcc gctggctccc accgctttca ggaacagcgg aatgctcaga atccccaaaa
     7621 ccgccagcag tatagaactc gcggcggatg agaagatcgc ggtggtggca gtttcatggg
     7681 cttcctgata ctgctttttg cccagcagcc tggaaagata gctgccgccc ccgttgccaa
     7741 agatgccggc gattgccatt tggaaagtga agagcggcag agagatcgat acggccgcca
     7801 cctggtaggg atcgttgagc ttgccgataa aaaaggtatc cgtgaggttg taaaggatat
     7861 tcaccagcat gctgagcacg ctgggcaaag ccaggtggat gatggaacgc gtaatgggca
     7921 tctgttccag gacgtagagc ttctcttttt tcatgcttgt gatatatttc cttgcgagtc
     7981 aaggaatttt gcggggttat tctgtcaagt caaacccccc ttcccatact tgagttatct
     8041 gtggccggtt tgaaaatcgg cagactttga gctttgcttg tcaaatggaa caatacttgc
     8101 tcccaagtag actctgacac aaaggaggct caagatgaga agagtgctta tgtcaatgct
     8161 tatgctgttt atggcaggct ggctgcttgc cgaccaaaac gatccgcccg ccaagggcag
     8221 atttgaattt tttccgcaaa gcccctaccg ggtttcaaaa ctggagcagc aggccaaagg
     8281 tgcagaccgc accacaatct ggagcaccaa cttcagcagt tccaccggct gggacctggc
     8341 ctacgcctgg aatatgggca cacccaccat gggaccggac accgtcccgg atggcaatgg
     8401 ctgtctggcc accaacctgg caggcttcta tgccaatgat tcaaacatcc gggccctgag
     8461 ccccagcatc ccaattcccg ctgccagcta tgtggaactg agtttcacgg agtggtttca
     8521 actggagagc gattacgact ttggctgggt ggagatcaaa cagggcagta cctattacag
     8581 cgtcgatgcc cgcagcggta cgagcggagg tcagtggcgt gaaacttcca tagatgtcac
     8641 ccgctggcag ggtagcagca ttcaggtggt gtttcacctc acttccgatg cctcggtccc
     8701 ggctttggga tggtatctgg actgcgccgc catcgatatt gtagagcctc atcccctgga
     8761 actggacatc accggcatca atatctcaaa cctgccttcg gtctacctga ccgcctcggt
     8821 gcgctctcct gatggtccgg tcaccactct cactcaatcc aatttcagcg tgtgggaaaa
     8881 ttccagcgct cagacgaatc tcttcagcgt catagctcct gataacagcc agaccgccag
     8941 ctccaccgat atcgtgttcg tgctggatgt gacaggcagc atgggagacg agatcgcttc
     9001 cgtgcgggcc aatatgcagg catttatgaa ccacctgcag ggccagaaca tggactatcg
     9061 catcggcttt gtggtctttg gagatatcgt ctatgtttac aatcaatact tcttttatac
     9121 cagcttcacc gagatcatga atatcatcag caatatcgaa ctcggcgaac ataacatcgg
     9181 aagcggagga gacggcccgg aaaaccaact ggaagcgatg gccgaaggca gcgtattcag
     9241 ctggcgccca ggagcttcca gagtgatgat catgctcaca gatgccaccg ctcacgaggc
     9301 tgactacgtc acggactgga ccgtcaacga cctcctggcc cagaggcttc tgcccaatga
     9361 agtgatggtg tttccgatct tcaacaccaa caatagcggt cagttggccc agtatttgcc
     9421 tatcgcccag cagactaatc ccagtgccgc ctgctattat atctacgata atttcaatga
     9481 catcatctca gagattggga actatataac ctctctctat acagtgcatt acatatctcc
     9541 agtccaggcg gatgacccca tgagccggat cgtgaaactc accgccagca gtggggccga
     9601 cagctcagag gattatgcct tctatctgcc tgggatatcg ccaatcatca cacgcgaagc
     9661 ggaactggaa gccctggact acactcccgt gcccgctgtg tggccactgg aactggaagt
     9721 ggtgattgag gaccgggtgc cgccaggcct cctcactcaa tccatcatat ggcgccacgt
     9781 aggcaccagc gcttggtatt cagctccgat gaccatgatc aaggaagatt actatcaggc
     9841 catcctgccg gccaaccagg tctccggggc tggaattgag tattacatca gcgccagcga
     9901 cggtcagacg agcgttactc tgccttccag cgaacctgag ctgcatcctt tcagcatcgc
     9961 ggtttctcct gccgggcatg ccagttttgg cactcccacg gccagctatt cctcctcgag
    10021 tcaactggca gtgagtgtga cggccacggc tttcaccact ccccagctca agctgtatta
    10081 ccgtcccatg ggtgccctga tctatgagga agcggctatg gcccagagcg gcaacaactt
    10141 cagcgccacg atcaaccaga atctcggctc cctgggagtg cagtattaca tcctctgcac
    10201 tcaggccaac cagttgatca cctatctcgg caatcccgat gagcctctgt atgtgacggc
    10261 tggcgctttg gcccctcaag tctctgatgc cgaatcgctt atctttgatt ccagggcctg
    10321 gcccaatccc gtttcgttat cccaacgggc cggatatgtg agcatcggtt tcagcctcaa
    10381 agaggaaaca tcagtccagg ccagaatcta caacctcaga ggtcaacttg tcaaagaatt
    10441 gtctgacaaa catctggttc ctggccagca caccctctgg tgggacctca aagacaagga
    10501 gggcgcacgc gtctccagtg gagtctatct ctatcgcctt caagccgatg gctatgccgc
    10561 cagcggaaaa ctgctgatcg cccaatgagg aggatatcat gaaaaagctt agtatattcc
    10621 tgttgctcgt cctgctcgct tacagcctgt ttgccaatgg cgtcctgatc aaaaacggca
    10681 tctccggtca gtatctgacc atcacaggct gccgggtgga gacggtgatc accaatcagg
    10741 tggccaccac cgttgccacc atgcgctttt acaacaacta cagcgtctcc accagcaccg
    10801 tgtttgcctt tcctcatccc ccggaagcca gtcccaccgc tctcagctgg tttgtcaacg
    10861 gcgcctggca tgatgccgtg atcgagcccg gagcgggagg ttcggtagtc ccgcccggcg
    10921 aagatatgta ccccgcttta gacaactatc tgggtaagac ctatctggct tttccggtgt
    10981 cggagcatgt gcagcccgct gctgagatga tcttccgcgt cacctatgtg cagctgctgc
    11041 cctatctgaa cggccgggtg gagtatgact tcaacagctc ctacggctat ctgaccaatc
    11101 aacccctgga ctcactgagc gtggatattc agatctccgg aaaccgcacg atcagcaatg
    11161 tgctgctgcc aggcttcacc aatgtcaata tcgacatcga cggcccgacc gcgtcagtgg
    11221 acctgatggc aagcaccttc aatctggacc tcgatatcga gctcagctat gatctggcca
    11281 tggaaaacct gggggccagc accttcagca cctttatcga ccatcaatac gtgcctgacg
    11341 acctcggcga cggcttcttt atgagcattg tggagccgca gcccagcgcg gacgtgatcc
    11401 agaaatactt tacctttatg ctggaccgca gcggcatgat gaacgacgcc agcatgcagc
    11461 aggccaaaga cgcggcctcc tatatgatcc aaaacctcaa cgccggcgat tacttcaaca
    11521 tcgtggattt tgccaccgtg gcgggaactt ttgccgtgaa ccacgtaccc ttcaatcccg
    11581 ccaaccgcga tctggccctt tcctacattg ccaatctgga gccagagggt tcctgcaaca
    11641 tctccggggc cttcgatacg gccattcctc agtttggcgg cgctccggac gaagcggccc
    11701 acgtgatcgt gtttctcacg atcgggcatc ccagcacggg gatcatcgac acccctactc
    11761 tcgtggacca tatcgacaac ctcgtcacgg ccacggatag agacgtgaat atcttctgct
    11821 ttggagtggg caccaacgta gccttccaac tgctttcgat gatctcggca gcccatcagg
    11881 gcatcgccac ctacgtggga ctcaatgaac tgctggatgt gctgatcgac ttctacacca
    11941 agatccgcaa ccccatcctg ctggaccccg aacttgaagt ggtgggccag accggcaaca
    12001 tcacacaaat ctatcccgat ccgctgccca acctctatct gggcaatcag atgatcctct
    12061 gcggcaggta tcatcaagcg gccaacctgg tcttcaacat aggcggctat gtgcttggca
    12121 gccatgtgaa ttaccactat gagcacgcgc tgggccagac ctcagagcct ggcatgcagt
    12181 tcctgatgaa gatctgggcc aagctcaaga tcgagcatct gatggcgctc tactaccagc
    12241 tggaccccta ttcccctcaa gcgatcgcgc tgcatgacca gatcgtgcag atcagcatcg
    12301 attatggagt gctgtgcagc ttcaccagtt tctctggtgg agaagtggcc aatcccgaag
    12361 acaccgtccc gcctgctctg catcctatca ggatcacggc ttgtggcccc aatcccttca
    12421 aaaaccatgt ggacctcaag gtgatggtca acgacgccaa acgcggtccg ctcacgttga
    12481 acatctataa cgcccgcggt cagttaatcc gtagcatcat aatctatccc gataaagctg
    12541 gagaatatat cttccgatgg gatggacgcg acgcagccgg cagatctgtg gccagcggag
    12601 tatatctctg ccgcattggg gtgacgggat tcagccagac ggccagagtg gtattgataa
    12661 aataaccccc tcaaccagtt gtgaatagca gacgcgggcc ctcaggtccg cgtcttttga
    12721 tagtggataa catgtgctgg ttgaagtcca agcggcccgc aaactcgttg tcatgcatac
    12781 ctttatcgtg gccgcttgaa ctccaacggg tgtatgtcag tctccggctt caccagtttt
    12841 cgcaaactcc tgcttcctgc cagcgcgctt gatctgtcaa tccagcttca aaaccctgac
    12901 agtcttggcc ttagcgccat gacccacccg gataaaatag atcccggacg cggtggaatg
    12961 acccgaggag tctttgccgt cccaagagaa ggtgagtttg tcaccatcgg gaatagcttt
    13021 gaggccacgg actttctggc ctctgaggtt gtaaacctcc gctttcatga aatcggcttt
    13081 gccagacgtc acactgaggg ctacggaacc gcggaaggga tttggccagg ctttcagttc
    13141 cggaagcctg gcggcataga ggctggccga ttgatagttt cggactctga tctgcccctc
    13201 gtgggtaccg gcggaaacgt ctatccagct ataagttgcg ggtgtcagaa ggcggatttg
    13261 tccatccaga tagagctcca cttcataact ggcgggtagg tctgaactgc agtaactgaa
    13321 ggccaccgga ccgctgttgg ccacttccag attgaaggtc caggttttgg tcacctggtc
    13381 gttatcggga tacaagcctt tgatctcact ctgcagaggg cctgtcgcga ccgtcccaag
    13441 cgccaggcta agctcctgag cgggcatcag aagggctttg gaaagatcaa actgcgggtc
    13501 ccaggtctcc gaagcgtcgt ccgccactcc gatctcgacg ccatcccggc ctctgaatcc
    13561 atcccaggcg ctcaggctca agctccaaag cagtggccag ctattcactg ccgtgccaga
    13621 tacttgcggg ttaaagatca ggttcagggg ctgagagccc tgataccaca tgaggaccgc
    13681 tgagaggggc tcgatatggt ccgtgagagc ccatcccgtt tcagagaaag cgtaaagccg
    13741 gggcgagatt atttcctggg ccacgagctc gtcccatgag taggaagcgc ctgccaggtc
    13801 gaaacttagc tgtcccagcg cgtagcgatg gtagtgagga ttggccacca gattccagcc
    13861 atggttcaga ttctggtaag ccaggcccag gttttcggtc atcggaaggc tcagttgcca
    13921 ggcttgaggt ccccacacca gatatgagtg gttacctgta agcgtgtcaa cgttttgcca
    13981 attggccacg ctgtccagtt cccagactgt ggtccccgcg gaaaataact catccacccc
    14041 agcgctccag cccgctatag gaatgcagat caggctgtgc cccgccgctc cattgtaaac
    14101 catgttatgt ggaagcagat catacagata gggagattcc cctctcaact ctgagccgtc
    14161 ggtggccagg cccttgacca ccagccggga tccgatcata ggcaagcccg acccactcca
    14221 cacaaatgcg gtttgggtgg ggcttagtcc agtgcagacc aggatactgt cttcagggcc
    14281 caccatccag agctctacgg atgtcactct ggcgggattg accacctgcc agttcagagt
    14341 cacaggcgtt ccggcctcaa agcgctgcaa acccagggct ggaaactcaa tccagggcgc
    14401 tttgtagccc caccagagct caaaagtcct gtagccagag gaagtggctg tccaggtgag
    14461 gggaccagtc ctgaagtcgt gccagagacc gctgtagttg tctttgatca gcaggtcccc
    14521 ttcctggggt gtgacgttaa aatcattctc cagcctcaac tgctgaccgg ctaaggagtt
    14581 gaagactttg acggtccatg gtttggagtc gctgtgcgga tagtagggag ctttgacgtc
    14641 ccgggccaga gtggccgccg gatttgccaa agtggaagcg atccagacag ttgaaaaccc
    14701 cggaccgggt ttttcgatgt ccagagcgtc ttgtgcatcg gtagcgaagg cattgacagc
    14761 gattttgagg ctgtcggcct ggccgttgcc gaggtctctg aaataaagag tgataggttt
    14821 aaacgcatgg gctgagagga tttgagggct ccagtactct gatccgtccg cgtaaacggc
    14881 gctgagctga tagcgataga cctgtccgtt ggtggcggcg ttgtcccaaa actcatagac
    14941 gccctgctgg ttgtgctgca gctcgggatg ctcctccgcg gtggcgtaag cagagatcgg
    15001 ccctgatccc gtgccccggt tgacgttata acccaccgcg gcggtgatgt tggcctggaa
    15061 ggatactttg atcctgccgt tatccggttg gagagcaaac tgcgcgaaag atcccattcc
    15121 ggacgcggga cgaaacacct tcagctcttc cgtccaggcg ctcacgttgc cgtgtttatc
    15181 tctggccctc attctcacat agatcattgt tgagttctct gggaaagaca gcatatactc
    15241 gttcaggggc tgccaggcca ggtcttcata gaggttgcgg tcgctttcgc tgagttgcca
    15301 gctcacccca tcgtgccagc gcgtctggat ctcataagtg tcgaaatcgt aatcataaga
    15361 gcgctgccag gtgagcacgt aagcggaagc acttactccg ctgatcctga aattttcggg
    15421 gttggaaggc gcgttgccgt cgttcatcat caacagctcg tccaggcaga ggtccagagc
    15481 ctcgatcacc ggccagtaaa aacccaggaa cctggtgtag cgcgtcgcga gcggccagcc
    15541 catttcctcg gagtaaccat agaacagatc ccgctgatca ccgctctgat caaagcagcg
    15601 gggcagttca tccatctcga tatgcaggaa ggggctgtag ctgtcataga ggttaaactg
    15661 ctgggtgaaa agcatctggc agttttccga agcgccgggg aggtcggtgt tttgaggaat
    15721 gaaagcgctg atgccatcct gctcatagta gatttcataa gccgaagcgc gatagatgct
    15781 gtaatactgg ctcacatcga cggcgtcgtg agtgccgaag ctgttggctt ccatcacttg
    15841 ccagggcgtg ttgtgaatga ggtcgttggg agcgcgtcga tggtctctga ccggcagggt
    15901 gggatgcagt cttccgtttc cggccgacag catcacggga gccttgttgg ggtatttatt
    15961 ccagtcgtag gtgtgcaact gcacgctgaa ttccgtccgt ccggtgagtc cccgtaactg
    16021 agtggcggcg tgctggtagg tcacgttgaa gggatgatcg gcaaagcggg agggatcgct
    16081 gatcgattga ttgttggagt ggtagggggg aaagtaggcc acttctctac ccgctccggc
    16141 caccagcaaa aagcgcgcgt cccaggccag gagggcctcg atactgatca cggggctggg
    16201 ataatcgtcg cagggatgcg gcgctgtgat catcatcggc cgtgaagcgg aagggtcata
    16261 gatatatagt ccccagccat aatcgaagct gccatattca tacagctcag ggtcttcgga
    16321 gtcgttggtg tctagctgag cgttgatctg ctccctcagc atgtaaaaga gccggccgct
    16381 atccgtatcc tgaaactgca caacgtcata ggggaagccg cagctgtcga tcacagcctg
    16441 ggccccttcc aaatccagag ccagccaggc ctgcaccacc tgagcccatt gattcagctc
    16501 ttcagtgctg gggatgtggt aatccccaaa gtcgttgttc tgctcgtccc agggagcgta
    16561 aacgttcagc cagctgacct ttccctccgc cacgtgagac acccagttgt cataggcaca
    16621 ggctggttcg gagccataga gaaatccgcg gatggatccc acttccgtgc tcagagcact
    16681 caggccaacc cacagcagca cagccgcgag cgtaagtatc cgtcgcatgg tttctcctcg
    16741 attttctctt tattccaacg tatctttgcc ctgcattgcc tgtcaagcag gaagttttcc
    16801 aaggtcaatc gaacaagcat gtaaatcccg gaatgcgaag tgcgatccct ctttccagcc
    16861 cgttgtgtag caaagagttg tctgtaagct ggattcccgt ctccgagctt gtgcgaaaat
    16921 ggcgtataga gtcccgtcag gggcgatatt ttagatagac aacccgctcc ctctctcatg
    16981 taactagccc cttcaggggc ggcattttag atgcgttgag cactgttagg caacggcttc
    17041 tgatgtgagg caagaatctg aaatatcgcc cctgacgggg ctcggacctg tgtctggcac
    17101 acgtaatcta gctaaaatat cgcccctgat ggggctcaat gcgccatttt cacacagcct
    17161 cctccccggg aatgacagca aggcttttca tgtgaactcc acttgatccc gttggagttc
    17221 aagcggccac gataaagcca tgcactacag cgagtttgcg ggccg
//
LOCUS       DAOSFN010000043        16728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2800_length_16728_cov_9.385414, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000043 DAOSFN010000000
VERSION     DAOSFN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 16728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16728
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2800_length_16728_cov_9.385414"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3757)
                     /locus_tag="PLO35_04310"
     CDS             complement(<1..3757)
                     /locus_tag="PLO35_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOH78921.1"
                     /translation="MKRNILFALLLIMGIGFCGNVFAQINVTIGNGTATNTTTGGPAP
                     YGTWYKNFRQQFLVLASEIEDAGGGPGNINSVAFNVAALNTCSPMTNYTIRLKTTTQT
                     ALTTTFEEGAYQTVFVQDGFLPVEGWNTHTFTTPFVWDGSSNILVDVVTSIIPGDWTQ
                     NASVYYTTTGFNSSLRYQSDSTEANTATTGSVSTNRSNIRFNMQALVVDDPPNPALIV
                     SPLDGAVNVMTSATLNWASGGGAPTGYKVHFGTTNPPPFIQNTTETSYTPTLATNTTY
                     YWKIIPFNAEGDAVNCPVWSFNTGGPVVLMTNGQQSIPDAMTYSFYDSGGPDGQYQNS
                     ENYTFTFSAANPTSIIHVMFTAFNCESGWDYLKIYNGPDISAPQIGPAAGYTGTTMPE
                     EIIGSNAVTFVFTSDSSVPYDGWAAAVSAVNLAHDLGAQSISGNTAPSVGTPTTYSIA
                     VKNNGSNTETTYTVKLKSSTGTELASVPGTSIAPQQIVNFDLTWTPSATGAMMIYGEV
                     VLANDGIATNNQTPNLNIAVQAAGVVAVTIGDGSAAARMPLDFFYKNSLSETIYPATD
                     MNIGGLITGVQYYNDFTYAAGLFAKPTKIWMGETAQTDLSGGWIASTNLTQVFDGVVD
                     YPTGQNNINITLQTPYVYGGGNLVVMAQRPMDTGWFSGVNFRTQTIGTARTLNVYSDS
                     TPFDPTAPPAGTAPTGMFPRTTFFLIVEDMGALTGTVFDGTTPLPGATVSITGTPLTT
                     TTDANGVYNFPYVAMGPQTVVATKHGYNVVTHAVTVIEDETVTQNFVLTQLPQVTVSG
                     RIVGSDQPTVGLANATISLTGYEPYTGTTNASGVFSITGVYASQTYEYTAHATGYQNA
                     TGQAVVGTTNLNMGDIIVNELAFPPLQVVATEAVNFSNVAVTWMAPDPNAVGEWIHYD
                     SGENNDSIGTGAAADFDVAIRYPASALTDYAGMSLYAVKAWPAQAGTFSVRVWTGGNA
                     DAPGAMVVDQAFTPAPLDAYNTIMLADPVTITGAEELWFGYRCNVTGGYPAGCDAGPA
                     TDGFGNMMYYQGAWSTLIELAPTLNYNWNIQGYVGFSAPSRAPELTPLASKPSFFDEA
                     SNVDRVLGGYRVYRLLAANQGNEALWTQLTPANITPTNYTDNAWAPLPSGVYKYAVKA
                     VYTNNVMSTPSFSNEIHKGMMGTLMGTVTEFGTNVPIEGATITAGDYSGTTNAQGQYS
                     FGVYQGTYNVTCSKVGYQAASQAGVVIVGLQTTTQNFVLTEITLPPGG"
     gene            complement(4089..4844)
                     /locus_tag="PLO35_04315"
     CDS             complement(4089..4844)
                     /locus_tag="PLO35_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78922.1"
                     /translation="MKYCLIVIILTLICHSAAFAEEAKRDPLSAEELFQQGLDLFEGQ
                     KTAKDIPAALQAWKDAADSNHVVAMYNLGVVYANGLDVTPDPALALHWYTRAAELGNS
                     DAMFNLGVLYGDRELAEMDSLKAIHWYERAGEAGDMRAQHNLGVMYEYGDLVAVDSLK
                     AASWYLKAAQQGMGEAQLKIGRWYCQEGVFKRNPMLAYVWLWLGSVNCRNQSGWIYED
                     DLQACEELLDDWQIEQARAVIEAVMECYERNRR"
     gene            complement(4991..5428)
                     /locus_tag="PLO35_04320"
     CDS             complement(4991..5428)
                     /locus_tag="PLO35_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HSP20 family small heat-shock protein"
                     /protein_id="HOH78923.1"
                     /translation="MKLVPYRRTNEIRPMSNMLSLFDEFFNRAFDEESNDENFRAMAM
                     DIVEHDKEYEILANLPGFKKDNVKISIHDNQLLVEATCDQKKEDRQGTMYRCERYSGS
                     YRRNLLLPENADFNKISAKMEDGILRLVIPKKEPSPQKEIVIQ"
     gene            complement(5569..8262)
                     /locus_tag="PLO35_04325"
     CDS             complement(5569..8262)
                     /locus_tag="PLO35_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOH78924.1"
                     /translation="MRVFKTFILMMATLGALSLTAAPHKALPIQVKQPDGRALTVYAS
                     GDEFHNWLHDENNFTIVRSDNGAYVYARQDGENVAPTGMLVGIDNPASRNLEPGINLS
                     PNLIRQKYDSLAHMRDYSNGRSPHTGQFNNLVIYIKFADDPDFSSPITYYDQMFNEEG
                     ENANSMKHYFQAASYNQLSVDSFHFPAPNGNSIVCYVDTHTRDYYRPYSAANPEGYSG
                     DSQRTQREQQMLMRAVTAVGPAIPTSLVIDGDNDNYVDNVCFIIQGSPDGWSDLLWPH
                     RWVLYAADAYIHGKQVWDFNFQLETSLFSSGASVLAHEMFHSLSAPDLYRYDDNTITP
                     IGSWDLMASNTNPPQHMSAWMKYRYGQWITEIPSITSSGTYTLSPVASSATNNFYRVN
                     SWRSNEYYVLEYRKPHGMYDGTVPGSGLLVYRLDTRESGNAQGPPDELYIYRPYGTNT
                     TTNGVLSLATFSEQSGRTELSEATIPNGFMGNNSSGGLNLYDVGMAGDTISFKIKISD
                     IQLTAPHGGEVWFSGTNKQISWKSKSTAGTVKLEYSTNSGADWTQIISGAPNSGSYTW
                     YNVPAMNTTAAHIKITLLTNNHEDSNVYPFSVISELAVPQGVYPSDGATGVPTNPQIS
                     WQSVAGASGYQFQLSSQQDFSSFIVNVIDHPESHYQLSGLQPNSHFYWRVASIGDIGI
                     SPFCETQHFQTGTISELPGVPALMLPAHMATGLTPPITFSWVASSLAESYLLQISTSP
                     YFAGSVIEVNNISGTQHSVGNLVPYTVYYWRVAANNVAGTSHFSQIRRFSTQQSSDSD
                     DPGTPVLDNELGPNYPNPFNPSTTIRLSVTDLRAPLSLEIFNVKGQLVRRLHSGLPKS
                     PRLSVVWDGRDDLGQAQPSGIYYYRMRSGAFTETRKMLMLK"
     gene            complement(8262..8615)
                     /locus_tag="PLO35_04330"
     CDS             complement(8262..8615)
                     /locus_tag="PLO35_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78925.1"
                     /translation="MKDTLPNQLGMSESAYQYLRGKDRFFASRARFARIVLNGVKCSG
                     ASFSLFHDFASDEDLRLRYKELTVLVEPEILERFGGFELDTERFFFATRILIKPRIDD
                     RNCDCNHKCDNNKEE"
     gene            9063..9938
                     /gene="dapA"
                     /locus_tag="PLO35_04335"
     CDS             9063..9938
                     /gene="dapA"
                     /locus_tag="PLO35_04335"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOH78926.1"
                     /translation="MLKGSYVALVTPFKNGEVDYSALEALLDFHLNKGTHGILLLGTT
                     AETAGLASDEKDALLRFAIHKLNRRVPVMIGTGTNNLHQTLANTMKAKELGADFALVI
                     TPYYIKPTQAGMYEYFGHIASRVDIPIVIYNVPGRTGVNILPATVVKLAKAYPNIVGI
                     KEASGSIVQATEIIRDAPDSFVVMCGEDAINLPLMAIGAKGCISVTANVVPGLMSEHI
                     QTCLNGDMSSAAVQHRRLQAINSVMFIETNPIPAKEALHMMGLINLEFRLPMCPLQDA
                     SREALAQVLKAYSLI"
     gene            10035..12959
                     /locus_tag="PLO35_04340"
     CDS             10035..12959
                     /locus_tag="PLO35_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH78927.1"
                     /translation="MKKPALKHGFVLKDSQEIGELKLSAHVYEHQKSGAELIHLACED
                     SNKVFCVAFKTIPEDSSGCPHIIEHSVLNGSRNYPAKNSFMELIKGSLHTFVNAMTSS
                     DMTIYPVASTNDKDFMNLSRVYMDAVFFPRIYEQPEIMHQEGWHYELNDPEGELKIRG
                     VVYNEMKGAYSSPDALIRYGSNQAQFPDTPYGLSSGGDPEVIPQLSYEKFLDFHRKYY
                     HPSNSRTLVYGNLDIDALLEMMDTDYLSHFDRDPEAVVMPAQKPFSKPVEKLVEYPID
                     EHKAPDGMYHLSLNYTWGSQSEMLDTAAFSVLSELLMMSTASPLKRKIMESGLAGDSY
                     AAPNTDILQPSLSIICKHVKEENLQPLVKLINTELKRIAKEGFDKKLIEAVLNSREFF
                     YREGQMQNFPKGLYFAWTMLGPWMHGADPLHPLRFEALLKDLRKGLTEPYYEAILEKA
                     LIKNKHHSIVTFKPVPGLLQTKESALKAELAKRKAQMKAPEIEALVKFNQELQTWQME
                     DDSPEDIARIPALSLDDIDRKAAEYPMEVEVWKEFTLLKHEVNTNGIVYLKAYFDLAH
                     ADEEDLPWLALYAYLAGKLDSENYSYADLANEIDIHTGGIDLRFSLLNSYQDPDVILP
                     KYIVAGKAVTAKSAKLMELAAEYALKPDFADHDRLRTLIRELKTRVESQLMMQGVMVA
                     INRMFAPFSQIHKWRDHSGGLQFFHWLVELETRLNEDIEAIIGELEWVRKTFFSQNNL
                     IISLTAGSDEIAEAFKHLVPVVANISSEVYAPVEHHFNTLNVNEGIMAPVQVQFCAKG
                     GNFFRKGYSYSGKLRVLNNVLSNEFLYKELRVKGGAYGAMSNFSLAGYLYFCSYRDPN
                     LRETLEAYNLVPDYLRSFDCSKRDMDKYIIGDISSLDYPKIPEAIGAQADEDYITGFT
                     QADRQQIRNEVLSTSVADIRSYADMIEAIMSKDHYCVFGNEEKVREAAELFNQLTPVY
                     PG"
     gene            13031..14095
                     /gene="rsgA"
                     /locus_tag="PLO35_04345"
     CDS             13031..14095
                     /gene="rsgA"
                     /locus_tag="PLO35_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOH78928.1"
                     /translation="MKQKDKKQPTPYSGRLRAISLPDVDVLDEGRESAHYKTQRAYSK
                     HAESFKEGLELIEGRVSEIHSNYAYIIESGGTYYNATLSGRLKQFLYGSQALAAVGDR
                     IQLDISQAPIYRVEHILPRSNTLSRYAGGSFQKEIVIAANIDQVIITSSWRMPMIKPG
                     LIDRYICIAALEGLKPVVVINKIDLCEYPEDLEEATDYYRRMGYSVILCSTLSGEGME
                     ELRQILKDRDSVFSGQSGTGKSSLINYLEPSLKLPTAEVSSFNEKGKHTTTQATLIPW
                     SFGGHLLDTPGIKTINLHANAKADIPHVFPGFESFAPHCRFRDCTHSHEADCAVLRAL
                     EEDVVPIERYDSYLRIMESL"
     gene            14095..14949
                     /locus_tag="PLO35_04350"
     CDS             14095..14949
                     /locus_tag="PLO35_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOH78929.1"
                     /translation="MQHFAVYLNPATDPVPIFKLLEQLGSQFNIRFWGTSAQRELLPH
                     YIKCCDPERGQMPRIDCVIVFGGDGTILRAKSLAIRTGAPILGVNLGYLGFLSETTLK
                     ELGASIQTLIQKRYKLISRMLINCQLRRDGKLIFQGLALNDAVIYKAETPRMITVRTY
                     SGGRFVFSARCDGVIAATPTGSTAYNLSAGGPLLTPEMRAIVVSHLNPHLLSIRPMVF
                     PATDKLCLKVTGLHEPAWLQIDGENVHQLKEMDELIVSDGRHQVEFVKLSNRTFFRIL
                     RNKLHLGK"
     gene            14949..16637
                     /locus_tag="PLO35_04355"
     CDS             14949..16637
                     /locus_tag="PLO35_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOH78930.1"
                     /translation="MLSAIRIQNYLFVPEARLEFGPGMTVLTGETGAGKSILVGAISL
                     IFGDGAYVPEPYDSSLPIYLEADFRIGKNSSLIAKLEELGHADSDELTLARQISVNGR
                     SVFYINGRKVAATLMKELRPLMIDFHHQRDQQRLLQASFQLEVLDAFAGTTSTRLQHG
                     SLLLQLRSRIKQLEELRLERERNAQLDELYRFQLEELEAAAVKTGEDIDLQHQYERLS
                     HTREIRETGDWVRHNLYEKEDSARDLIRSAINSLERFSILDERVAQLVQSLHDSLQIL
                     GDAAPEAEALCSASEDDPERLETIRLRLDQINSLLYKHRVKDIAQLLDLFEQRRLQIG
                     SFDALDGQIASLDAGIRTSFTELVLTGEQLGRQRAEAASRLSSELQNAIRALAIPEAR
                     FEISIDKKASSEMLMSDYLAAISSSGADLCEFRFSANPGIGLRPLADVASGGELSRVL
                     LAIKKVLAGRLEPRLMILDEIDAGIGGKTAEAVAAVIRELAQAHVVLCVTHLASIAAV
                     ADWHLALDKVATGTRTLVSMRLLDDEARPRELARMLSGSVTEASLRHASELMNK"
BASE COUNT         3738 a         4517 c         4817 g         3656 t
ORIGIN      
        1 cgccacccgg aggcagagtg atctcggtga gcacgaagtt ttgagtggtg gtctgcaatc
       61 caacaattac cacaccagcc tgtgaggcgg cctgatagcc aaccttggag caggtgacgt
      121 tgtaggtgcc ctggtaaacg ccaaagctgt actggccctg ggcgttggtg gttccgctgt
      181 aatctccggc agtgatggtg gcgccttcga tggggacgtt ggtaccgaat tcggtcacgg
      241 tgcccatcag ggtgcccatc atgcctttgt ggatctcgtt ggagaaggag ggagtggaca
      301 tcacgttgtt ggtgtaaaca gccttgacgg catatttgta gacgcctgaa ggcagcgggg
      361 cccaggcatt gtctgtgtag ttggtagggg tgatattggc aggagtgagc tgagtccaga
      421 gagcttcgtt gccctgattg gcagccagca gacgatagac acggtatcca cccaggacac
      481 ggtccacatt gctggcttcg tcaaagaagc tgggcttgga ggccagagga gtcagttccg
      541 gggcacggga aggagctgag aagcccacat aaccctggat attccagttg tagttgaggg
      601 tgggggccag ctcgatcaga gtgctccagg cgccctgata atacatcatg ttgccaaagc
      661 catcggtggc aggaccggca tcgcaaccgg caggataacc gccagtaacg ttgcagcggt
      721 agccaaacca gagctcttcg gcaccggtga tggtcacagg gtcggccagc atgatggtgt
      781 tgtaagcatc cagcggagcg ggtgtgaagg cctgatcgac aaccatggcg ccaggggcgt
      841 cagcattgcc acctgtccag acgcggacgg agaaggtccc agcctgcgcg ggccaggctt
      901 tcacagcgta gaggctcatg ccggcatagt cggtaagagc gctcgcgggg taacggatgg
      961 cgacgtcaaa gtcggccgcg gcaccggtac cgatgctgtc gttgttttca ccactgtcat
     1021 agtggatcca ttcgccgacg gcattgggat ccggggccat ccaggtaacg gctacgttgc
     1081 tgaagttgac agcttcggta gcgacaacct gaaggggcgg gaaggcgagt tcgttgacga
     1141 taatgtcgcc catattgagg ttggtggttc ctaccacggc ctggccagtg gcattctgat
     1201 agccagtggc atgggcggtg tattcgtagg tctggctggc atagacgcca gtaatggaga
     1261 acacgccgga agcgttcgtg gtgccggtgt agggctcata accggtgagg ctgatcgtgg
     1321 cgttggccag gcccacggtg ggctggtcac tgccgacgat acgtccgctc acggtcacct
     1381 gaggcagctg ggtgagcacg aagttctggg tgacggtttc atcttcgata acggtgactg
     1441 catgggtgac aacgttgtag ccgtgtttgg tagccacaac ggtctggggt cccatcgcca
     1501 cgtaggggaa gttgtaaacg ccgttggcgt cggtggtggt ggtgagaggg gttccggtga
     1561 tggaaacggt ggctcccgga aggggagtgg ttccatcaaa aacggtcccg gtgagggcac
     1621 ccatgtcttc cacgatcagg aagaaggtgg tcctggggaa cattccggtg ggagcggttc
     1681 cagcaggagg agcagtagga tcaaagggcg tgctgtctga atagacattc agagtacggg
     1741 cagtgccgat ggtctgggtg cggaagttga ctccgctgaa ccagccggtg tccatgggac
     1801 gctgcgccat cactaccagg tttcctccgc catacacgta gggggtctgg agggtgatat
     1861 tgatgttgtt ttgtccggta ggatagtcca ccacgccgtc gaagacctgg gtcaggttgg
     1921 tggaagcgat ccagccaccg ctgaggtcgg tctgagcggt ctcacccatc cagatcttgg
     1981 tgggtttggc aaagagaccg gccgcgtagg tgaagtcgtt gtaatactgc acgccggtga
     2041 tcaacccgcc gatgttcatg tcggtggcgg gatagatcgt ttcggacaga ctgttcttat
     2101 agaagaagtc cagaggcatg cgggcggccg cagatccatc gccgatggtg acagccacga
     2161 cgccggcggc ctgaacggcg atgttcaggt tcggggtctg gttgttggtg gcgatgccgt
     2221 cgttggccag gaccacttca ccgtagatca tcatcgcgcc agtggcggaa ggggtccagg
     2281 tgaggtcgaa gttgacgatc tgctgaggag cgatactggt accgggaacg gaagccagct
     2341 cggttccggt ggagcttttc agcttgaccg tataggtggt ctcggtgttg gagccgttgt
     2401 tcttcaccgc gatgctgtag gtagtggggg ttcccacgga gggcgccgtg tttccgctga
     2461 tgctctgagc gccaagatca tgggccagat tgacagcgct gactgccgcg gcccagccat
     2521 catatgggac tgagctgtca gaggtgaaga caaaggtcac ggcattggag ccgatgattt
     2581 cttccggcat ggtagttccg gtgtaaccgg ccgccggtcc gatctgaggg gcactgatgt
     2641 ccggaccgtt gtagatcttc aggtaatccc agccgctttc acagttgaag gcggtgaaca
     2701 tcacatggat gatcgaggtg ggattggcag ccgagaaggt aaaggtgtag ttttcggagt
     2761 tttgatactg gccgtcagga ccgccggaat cgtagaagga ataggtcatg gcatccggga
     2821 tagactgctg gccgttggtc atcaacacta ccggtcctcc ggtgttaaag ctccagaccg
     2881 ggcagttgac agcatcacct tcagcattga aggggattat cttccagtaa taggtggtgt
     2941 tggttgccag agtcggagta taggaagttt cggtggtgtt ttgaatgaag ggaggtggat
     3001 tggtggtgcc aaagtgcacc ttgtatccag tgggagcgcc accgccggaa gcccagttaa
     3061 gggtggcgga ggtcatgacg ttgacagccc catccagagg agaaacgatg agggccgggt
     3121 tgggtggatc atccaccacc agagcctgca tgttgaatct gatattggag cggttggtgc
     3181 tcacagagcc agtggtggca gtgttggctt cggtgctgtc gctctgatag cgcagggagc
     3241 tgttgaagcc agtcgtcgtg taatacactg aagcgttttg ggtccagtca ccagggatga
     3301 tggatgtgac tacatcgaca aggatgttgg aggagccgtc ccacacaaag ggagttgtga
     3361 aggtgtgggt gttccatcct tcgacgggga ggaaaccatc ctgcacaaag acagtctgat
     3421 aggccccttc ttcaaaggtg gtcgtcaggg cggtctgagt ggtggttttt agacggatgg
     3481 tgtagttggt cataggcgaa catgtattca gagcggccac attgaaggcc actgaattga
     3541 tgttgcctgg tccgccgccc gcgtcctcaa tctccgaggc aagtacgagg aactgctggc
     3601 ggaaattttt gtaccaggtg ccatagggtg ctggtccgcc agtggttgtg ttggtggcgg
     3661 ttccattgcc tatggtgacg ttgatctggg cgaaaacgtt gccgcagaag ccgatgccca
     3721 taatcagcag cagcgcgaaa agaatgtttc ttttcatagg ttgtctcctt gtcctatttt
     3781 gttagggttt actcacatta acagagaact ggcccggacc cggatattca aggcccccgc
     3841 cgtctctatg gaattcacaa aatctaattc aactcatttc acactataac ttacgaatca
     3901 ccaccccggg gagtggtcat ccaaaaacac cattaggaaa actacgcata tcatcccgct
     3961 tttctgtcaa gcaagaaatc tgtctcttgg caaaaaagga tatacgcaag gagtttccgt
     4021 gttcaattgg gtagcaagat ttaacccata tcgccagaaa agtgccggtt tggggtatgg
     4081 accggacttt agcgccggtt gcgttcataa cactccatca cggcctcgat cacagctctg
     4141 gcctgttcta tctgccaatc gtccaggagt tcttcacagg cctgcaggtc atcctcatat
     4201 atccagccgc tttgattacg gcagttcacg ctgcccagcc agagccagac ataggccagc
     4261 atgggattgc gcttaaacac tccctcctgg caataccagc ggccaatttt gagctgggct
     4321 tcgcccatac cctgctgagc ggccttcaaa taccaagacg cggctttgag actgtccacg
     4381 gccaccaaat caccatactc atacatgacc ccgagattat gctgggccct catatctcca
     4441 gcttcgccag cccgctcata ccaatgaatg gccttgaggc tgtccatctc agccagttcc
     4501 cggtcgccgt aaagcactcc cagattgaac atggcgtcgc tgttgcccag ttccgcggcc
     4561 cgggtatacc agtgcagggc aagggcggga tcgggtgtca catccaggcc gttggcatac
     4621 accactccca gattgtacat ggccaccacg tgattgctgt ccgccgcgtc cttccaggct
     4681 tgcagggcgg ctgggatatc tttggcggtc ttctggccct caaacaggtc aagtccctgc
     4741 tggaaaagct cctccgcgct gagcggatca cgctttgcct cctcggcaaa agcggcagag
     4801 tggcagatta gcgtcaggat gatgacgatc agacaatact tcaaatcagg ctcccgtttc
     4861 ggtttactgg tagacagaca ctatcaggac aggacgggat ttttgtcaag cgcgaatcgc
     4921 aatgagacct gtaaccagca aaaaggcgtg ccggactggc acgccttggg atagagggag
     4981 tatggatgct ttattggatc acgatctctt tctggggaga gggttccttt ttgggaatca
     5041 ccaggcgcag gatgccatct tccatcttgg cgctgatctt gttgaaatcg gcattttcgg
     5101 gcaggagcag gttgcggcga tagctgccgc tatagcgttc acagcggtac atggtgcctt
     5161 gcctgtcttc cttcttttga tcgcaggtgg cctccaccag cagctgattg tcgtgaatgg
     5221 agatcttgac gttgtctttc ttgaatccgg gcaggttggc cagaatctca tattctttat
     5281 cgtgttccac gatatccatg gccatagcgc ggaagttttc atcgttgctc tcttcgtcga
     5341 aagcgcggtt aaagaactca tcgaacaggc tcagcatgtt gctcatgggc ctgatttcat
     5401 tggtgcggcg atagggtacg agtttcattg ttaacctcct tgaattagct cgtggttgat
     5461 atctacccca tattgtaatc ggggttggaa gaaaggcaag aaaaaaatta gcagtccgct
     5521 atgtggactg ctaatttagc cggaggggct gccttgcaaa aagaggaatt acttcagcat
     5581 cagcatcttg cgcgtttcgg taaatgctcc ggaacgcatc cggtaatagt aaattccact
     5641 gggctgggcc tgtcccaagt cgtcccggcc atcccaaacc acactcagac gagggctttt
     5701 gggcagaccg ctatggaggc ggcgcaccag ctggcctttg acgttgaaga tctccaggct
     5761 gagaggggcc ctgaggtctg tgacgctaag ccggatagtg gtggagggat tgaaaggatt
     5821 gggatagttg gggcccagtt cgttgtccaa caccggggtg ccgggatcgt cgctgtcgct
     5881 ggattgctga gtggaaaacc tgcggatttg agagaaatga ctggtcccgg ccacattgtt
     5941 ggcggccact ctccaataat agactgtata gggtaccagg ttgccgacgc tgtgctgggt
     6001 accgctgatg ttgtttactt caatcacgga accggcaaag tagggactgg tgctgatctg
     6061 caggagatag gactccgcca gggaactggc cacccaggaa aaagtaatgg gaggggtgag
     6121 gcccgtggcc atatgagctg gcagcatcag ggccggcact cctggaagct cactgatcgt
     6181 gccggtctgg aagtgctggg tttcgcagaa ggggctgatc ccgatatctc caatggaggc
     6241 aacccgccaa tagaaatggc tgttgggctg cagaccgctg agctggtaat gactttccgg
     6301 atggtctatg acattgacta taaagctgct aaagtcctgc tgggatgaaa gctggaactg
     6361 ataaccagag gccccggcca cgctttgcca gctaatctgg ggattggttg gcaccccggt
     6421 cgccccatcg ctgggataca caccctgagg aactgccagt tcgctgatca cactgaaggg
     6481 atagacgttg gaatcctcat ggttgttggt gagcagggtg atcttgatat gggccgcggt
     6541 ggtgttcata gccggaacgt tgtaccaggt atagctgccg ctgttgggag cgccgctgat
     6601 gatctgagtc cagtccgccc cgctattggt gctgtattcc agtttgacgg tcccggcagt
     6661 gcttttggat ttccagctga tctgtttatt ggtcccggaa aaccacactt cgccgccatg
     6721 gggagcggtg agctggatat cggagatctt gattttgaag ctgatcgtgt cgccagccat
     6781 gccgacgtca tagaggttga gccccccgct ggagttgttg cccataaagc cgttggggat
     6841 ggtggcttcg gaaagctcgg tgcgaccgct ctgctccgag aaggtggcca gagaaagcac
     6901 tccattggtg gtggtattgg tgccataggg acggtaaata tacagctcgt cgggcgggcc
     6961 ctgagcgttt ccactttcac gggtgtccag ccggtaaacc agcagtccgc ttccgggcac
     7021 ggtgccgtcg tacattccgt ggggtttgcg gtattccaat acatagtatt cattggaacg
     7081 ccaggagttg acgcggtaga aattgttggt ggcagaagaa gccaccgggc tcagagtata
     7141 ggtgccggag ctggtgatgc tgggaatctc cgtgatccac tgtccatagc ggtatttcat
     7201 ccaggcgctc atgtgctggg gcggattggt gttgctggcc atcaggtccc agctgccgat
     7261 gggtgtgatc gtattgtcgt cgtatcgata gagatcggga gcgctgaggc tgtggaacat
     7321 ctcatgggcg agcaccgagg cgccagatga aaacagcgag gtctccagct ggaagttgaa
     7381 gtcccacacc tgtttgccat ggatataggc atcggcggca tagagcaccc agcggtgagg
     7441 ccagagcagg tcactccagc catccggaga accctggatg atgaaacaga cgttgtccac
     7501 atagttgtcg ttgtcgccat cgatcaccag tgaagttggg attgcaggtc ccaccgcggt
     7561 cacagctcgc atcagcattt gctgttcgcg ctgggtgcgc tggctatctc cactgtatcc
     7621 ttcaggattg gcagcgctgt agggacggta gtaatctctg gtatgggtat ccacatagca
     7681 gacgatgctg ttgccattgg gagcggggaa gtggaaggaa tccacgctga gctgattgta
     7741 ggaggcggcc tggaagtagt gtttcatgga gttggcgttt tcgccttcct cgttgaacat
     7801 ctgatcgtaa taagtgatcg ggctggaaaa atccggatcg tcggcaaact tgatatagat
     7861 caccaggttg ttaaactgcc cggtatgagg agagcggccg ttgctgtaat cccgcatgtg
     7921 ggccaggctg tcatacttct ggcggatgag gttggggctc agattgatgc cgggctccag
     7981 attccgggaa gcgggattgt ctatccccac cagcatcccg gtgggggcca cgttttcgcc
     8041 atcctgacgg gcatagacat aggctccgtt atcgctgcgc acgatcgtga agttgttttc
     8101 gtcgtgcagc cagttgtgaa attcatcccc gctggcatag actgtcaaag ctctgccatc
     8161 gggttgtttg acctggatgg gcagtgcttt gtggggagcg gccgtcaggc tcaaggctcc
     8221 cagcgtggcc atcatcagga taaaagtctt gaatactctc attattcctc tttgttgtta
     8281 tcacacttat ggttacaatc gcagttacgg tcgtcgatcc ggggtttgat gaggatgcgg
     8341 gtggcaaaaa agaaacgctc cgtatccagc tcaaaaccgc caaaccgctc cagtatttca
     8401 ggctccacca gcacggtgag ctctttgtag cggagccgca gatcctcatc cgaggcaaaa
     8461 tcgtgaaaga ggctgaagct ggcccccgaa cacttcactc cgttcagcac aattctggca
     8521 aaacgggccc gggaggcaaa aaacctgtct ttgcctctca gatactggta agccgattcg
     8581 ctcatgccta actggtttgg aagtgtgtct ttcatagtac ttgatatctg ttacaatttg
     8641 tgttccaatt ttatcaggaa atagcagttg tggtctgcgg gagacaatct ccgctttctt
     8701 gcctgatcac ccgccgtgaa gatgccgtgc gctctcccgg gtttttcggc ggagagcgca
     8761 cggcatctcc acggcaacaa atgagggagg atacagcgcc gcctgggtgt tatatccccg
     8821 caagcctggg aacccagccg gctggacacg cagcgtttgg atccggatat ccatgctggc
     8881 gtgaatggca agctgacagg gattggctga atatccgctc cgggctgccg atttttattg
     8941 actaaaaaac tgatatctgc ttttttggta tgaaatttgt aatctgtaaa gcatggtagt
     9001 ccagaccatt taacgcctcg ccgggacggg ctgccaaaag cttgcaaaac aaggagctaa
     9061 tgatgctgaa aggttcatac gtcgcgttgg tgactccctt caaaaacggg gaggtggatt
     9121 attccgccct cgaagctttg ctggactttc atttgaacaa ggggacccac gggatcctgc
     9181 ttttgggcac cacagccgag acggctggcc tggcctcgga tgaaaaggat gccctcttgc
     9241 gctttgccat tcacaagctc aatcgacggg tgccagtgat gatcggcacc gggaccaaca
     9301 acctccatca aactctggcc aacacgatga aggccaagga gttgggtgcg gatttcgctt
     9361 tggtgatcac cccctattac atcaagccca ctcaggccgg gatgtatgaa tactttggcc
     9421 atatcgcctc cagggtggat atcccgatcg tgatctacaa cgttccgggc cgcacgggag
     9481 tcaacatcct tcccgccacc gtggtcaagc tggccaaggc ctatcccaat atcgtaggga
     9541 tcaaagaagc cagtggcagc atcgtgcagg ccaccgaaat cattcgggac gcccctgaca
     9601 gctttgtggt gatgtgtggc gaggatgcca tcaacctgcc cctgatggcc atcggcgcca
     9661 aaggatgcat ctccgtgacc gccaatgtgg tacccggact gatgagcgag cacatccaga
     9721 cctgtctgaa cggggacatg agttccgcgg cggttcagca tcgccggctc caggccatca
     9781 actccgtgat gtttatcgag accaatccca tccccgccaa ggaagccctg cacatgatgg
     9841 gcctgatcaa tctggaattc cgcctgccga tgtgtcccct ccaggatgcc agccgggaag
     9901 ctctggcgca agtcctgaag gcctattccc ttatataaag caataataat aacagcagtt
     9961 ggggccggag tccttagcca ggccgtaagt taagcctgga ccgccggtta ccgctctaca
    10021 ggagatatac aaagatgaaa aagccagctc tcaaacatgg cttcgtcctc aaggacagcc
    10081 aggagatcgg cgagctcaag ctcagcgcgc acgtctatga acaccagaaa agcggcgccg
    10141 agctgatcca cctggcctgc gaagacagca acaaggtctt ctgcgtggct ttcaagacca
    10201 ttccggaaga cagcagcggc tgtcctcaca ttatcgagca ctccgtgctc aacggttcgc
    10261 gtaactaccc tgccaagaac tccttcatgg agctcatcaa aggcagcctg cacaccttcg
    10321 tgaacgccat gacgtcatcg gacatgacca tctatccggt tgcctctaca aacgataagg
    10381 atttcatgaa tctcagccgg gtatatatgg acgcggtgtt ttttccccgc atttatgagc
    10441 agcccgagat tatgcatcag gaaggctggc actatgagct caacgacccc gagggcgagc
    10501 tcaaaatcag aggcgtggtt tacaacgaaa tgaagggggc ctactccagc ccggacgcgc
    10561 tgatcagata tggcagcaac caggcccagt ttcccgatac gccctatgga ctttcttccg
    10621 gaggcgatcc tgaggtgatc ccccagctca gctatgagaa gtttctggat tttcaccgca
    10681 agtattacca cccctccaat tccaggaccc tggtctatgg caatctggat atcgacgccc
    10741 tgctggagat gatggataca gactatctca gccatttcga ccgcgatccc gaagcggtgg
    10801 tgatgcctgc ccaaaagccc ttctccaaac cggtggaaaa gctggtggaa tatcccatcg
    10861 acgaacataa agctccggac gggatgtatc atctgagcct caactacacc tgggggtctc
    10921 agtccgaaat gctggatacg gcagccttct ccgtgctcag cgagcttctg atgatgagca
    10981 ccgcctcacc cctcaagcgc aaaatcatgg agtctggcct ggcgggagat tcctacgcgg
    11041 cacccaatac agacatcctg cagcccagcc tgagcatcat ctgcaagcac gtcaaagagg
    11101 aaaacctcca gccgctggtc aagctcatca acaccgagct caaacgcatc gccaaagagg
    11161 gctttgacaa aaagctcatc gaagccgtgc tcaacagccg ggagttcttt taccgcgaag
    11221 gccagatgca aaactttccc aaggggctgt attttgcctg gacgatgctg ggaccctgga
    11281 tgcatggggc cgatccgctg catcccctgc gttttgaagc cctgctcaag gatctgcgca
    11341 aaggcctcac ggaaccatat tatgaggcca tcctcgagaa agcgctgatc aagaacaaac
    11401 accacagcat cgtgaccttc aaaccggtgc cgggcctgct gcagaccaaa gaaagcgccc
    11461 tcaaagctga actggccaag cgcaaagccc agatgaaagc gcctgagatt gaggccctgg
    11521 tgaagtttaa ccaggaactc cagacctggc agatggaaga cgacagcccc gaagacattg
    11581 ccagaattcc cgctctcagc ctggatgata tcgaccgcaa agcggctgaa tatcccatgg
    11641 aagtggaagt ctggaaagag tttaccctgc tgaagcacga ggtcaacacc aacggcatcg
    11701 tgtatctcaa ggcctatttc gacctcgccc acgccgatga ggaagatctg ccctggctag
    11761 cactctatgc ctatctggcc ggaaagctgg attccgaaaa ctattcctac gccgatctgg
    11821 ccaacgagat cgacatccac accggcggca tcgatctgcg cttcagcctc ctcaacagct
    11881 atcaggaccc ggacgtcatc ctgcccaaat acatcgtggc cggtaaggct gtgactgcca
    11941 agagcgccaa gctcatggaa ctggctgccg aatatgccct caagcccgac ttcgcggacc
    12001 acgatcgcct gcgcactctg atcagagagc tcaagacgag ggtggaaagc cagctgatga
    12061 tgcagggcgt gatggtggcc atcaaccgca tgttcgctcc cttcagccag atccacaagt
    12121 ggcgtgacca cagcggggga ctacagttct tccactggct ggtggaattg gagacccgtc
    12181 tgaatgagga tatcgaggcc attatcggtg agctggaatg ggtccgcaag accttcttca
    12241 gccaaaacaa cctgatcatc agcctcaccg cgggcagtga cgagattgcc gaagccttca
    12301 aacatctggt gccagtggtg gccaacatct cttccgaagt ttacgctccg gtggaacatc
    12361 atttcaacac cctaaacgtc aatgaaggaa tcatggctcc ggtgcaggtg cagttctgcg
    12421 ccaagggcgg caatttcttc cgcaagggct attcctattc aggcaagctt agagtgctca
    12481 acaacgtgct ctccaatgag ttcctctaca aggaactgag agtgaagggt ggcgcttacg
    12541 gtgccatgag caatttcagc ctggcgggat acctgtattt ctgctcctac cgcgatccca
    12601 atctgcgtga gaccctggag gcctacaacc tcgtgccgga ctacctccga agcttcgatt
    12661 gctccaaacg ggacatggac aagtatatca tcggcgatat ctcctctctg gactatccca
    12721 agatcccgga agccattggc gctcaagccg atgaggacta tattacgggc ttcacccagg
    12781 ccgatcgcca gcaaatccgg aacgaggtgc tgtccaccag cgtggcggat atccgttcct
    12841 atgccgatat gatcgaagcc atcatgtcca aagaccatta ctgcgtgttt ggcaacgagg
    12901 aaaaagtgag ggaagcggct gagctcttta atcagcttac acccgtgtat cctggctagt
    12961 gggaccgctg acagcctggt ttggacctca tggattcgac agtgccatcg tttgtggttg
    13021 actgacaaac gtgaaacaga aagataaaaa gcaacccacg ccctattccg gcaggctgag
    13081 agccatcagt ctgccggatg tggacgttct ggatgaggga cgcgaaagcg ctcattacaa
    13141 gacccagcgc gcttacagca agcacgccga gagctttaag gaaggcctgg agctcatcga
    13201 ggggcgcgtc agcgaaattc actccaacta tgcctatatt atcgaaagcg gcggcaccta
    13261 ttacaatgcc actctcagcg gcaggctcaa gcagtttctg tatggcagcc aggcccttgc
    13321 ggctgtggga gacaggatcc agctggacat ctctcaggcc cccatctatc gggtggagca
    13381 tattctgccg cgctccaaca ccttgtctcg ttacgcgggc ggttcttttc agaaagagat
    13441 cgtcattgcc gccaatatcg atcaggtgat catcacttca tcctggagga tgccgatgat
    13501 caaaccgggg ctcatcgacc gctatatctg catcgccgct ctggaaggct tgaagcccgt
    13561 ggttgtgatc aacaagatcg acctctgcga gtatcccgaa gacctggaag aagccaccga
    13621 ctactatcgc aggatgggtt acagcgtgat cctgtgttcc actctcagcg gagagggcat
    13681 ggaagagctg cgccagatat taaaagacag ggacagcgtg ttttccggcc agtccgggac
    13741 cggcaagagc tctctgatca attacctgga gcccagcctc aaacttccca ccgcggaagt
    13801 gagttccttc aacgaaaagg gcaaacacac caccactcag gccactctca ttccctggag
    13861 ctttggaggc cacctgctgg atactcccgg catcaagacc atcaatctcc acgccaatgc
    13921 caaggctgat attccccacg tctttcccgg tttcgagagc tttgcccccc attgccgctt
    13981 ccgggattgc actcacagcc acgaagccga ttgcgccgtc cttcgtgccc tcgaggaaga
    14041 tgtggtcccc atcgaacgct atgactctta cttaaggatc atggagtcgc tgtgatgcag
    14101 cattttgccg tctatctcaa tcctgccacc gatcccgtgc cgatcttcaa gctgctggaa
    14161 cagctcggta gccagttcaa catccgcttt tggggcacca gcgctcagcg tgaattgctg
    14221 ccccactaca tcaaatgctg cgatccggag cggggccaga tgccacgcat cgattgcgtg
    14281 atcgtgtttg gaggggatgg caccatcctg agggcaaaat ctctcgccat ccgcaccgga
    14341 gcacccatcc tgggagtcaa tctgggctat ctggggttcc tctccgagac caccctcaaa
    14401 gagctgggag cttctatcca gactctcatc caaaagcgct acaagctgat ctccaggatg
    14461 ctgatcaact gccagctcag gcgcgacggc aagctcatct ttcagggcct ggccctcaac
    14521 gatgccgtga tctacaaagc cgagacccct cgcatgatca cggtgcgcac ctacagcggc
    14581 ggacgctttg tcttcagtgc ccgctgtgat ggcgtaattg ccgccactcc aaccggttcc
    14641 acggcttaca acctctccgc gggagggcct ttgctcactc ccgagatgag ggccatcgtg
    14701 gtcagccatc tcaaccccca tctgctttcc atccggccta tggtgttccc ggccacggac
    14761 aagctttgcc tcaaggtaac cggtctgcac gagccggcct ggctgcagat cgatggagag
    14821 aacgtgcatc agctcaaaga aatggatgag ctcatcgtga gcgacggacg ccaccaggtg
    14881 gagtttgtca aactctccaa ccgcaccttt ttccgcatcc tgcgcaataa actgcacctg
    14941 ggaaagtgat gctgagcgcg atcagaatcc aaaactatct ctttgtgccg gaagcgcggc
    15001 tcgaatttgg gccaggcatg accgtcctca ccggtgagac cggagcggga aaatccatcc
    15061 tggtgggggc catctctctg atctttggag acggggccta tgtgccggaa ccctatgact
    15121 cttccctgcc gatctatctg gaagcggatt tccggatcgg caaaaacagc tccctgatcg
    15181 ccaaacttga ggagctgggc catgcggact cagatgagct cacgctggcc cgccagatct
    15241 ccgtaaacgg ccgttcggtg ttttacatca atggccgcaa ggtggccgcc acgctgatga
    15301 aagaactgcg ccccctcatg atcgattttc atcaccagcg ggaccagcaa cgcctcctgc
    15361 aggcttcctt tcaactggaa gtattggacg cctttgccgg caccacctcg acccgcctcc
    15421 agcatggcag cctgctgctg caactgaggt cccggatcaa acagctggaa gagctcaggc
    15481 ttgagcggga aagaaacgcc cagctggacg agctctaccg ctttcaactg gaagagctgg
    15541 aagccgcagc tgtcaaaacc ggcgaagaca tcgacttaca acatcaatac gagcgccttt
    15601 cccacacccg tgagatccgt gaaacgggag actgggtccg gcacaacctc tatgaaaagg
    15661 aagacagtgc cagagacctg atccgctccg ccattaacag tctggagcgt tttagcatcc
    15721 tcgatgaaag ggtagcccag ctagttcaaa gcctgcacga cagcctgcaa atcctgggag
    15781 acgcggcccc agaagccgaa gcgctctgct ccgcttcgga agacgatccg gaacgtctgg
    15841 aaaccatccg tctgcgcctg gaccagatca actccctgct ctacaaacac cgcgtcaaag
    15901 acatcgcgca actgctggat ctctttgaac aacgacgcct ccagatcggc agtttcgacg
    15961 ctctggacgg tcagatagcc agcctggacg caggcatccg gacatctttt accgagcttg
    16021 tcctgaccgg cgaacagctt ggcagacaaa gggctgaagc cgcttcacgt ttgagctcgg
    16081 agctgcagaa cgccatccgt gccctggcca ttccggaagc ccggtttgag attagcattg
    16141 acaaaaaagc aagcagtgaa atgttaatgt ccgattacct tgcggccatt tcctcaagcg
    16201 gtgccgacct gtgtgaattt cgcttcagcg ccaatcctgg aattggactc aggcctttgg
    16261 cagacgtggc ttccggggga gaactctccc gcgtgttgct ggccatcaaa aaagtgctgg
    16321 ccggccgcct tgagcctcgt ctgatgatcc tggacgaaat cgatgccggg atcggtggca
    16381 aaaccgctga agccgtggcc gcggtaatcc gtgaactggc ccaggcccac gtggtcctgt
    16441 gtgtgaccca ccttgccagc atcgccgcgg tggccgattg gcacctggcc ctggacaagg
    16501 tcgccacagg gaccaggacc ctggtctcga tgcgcttgct ggatgacgag gcccgcccgc
    16561 gtgagctggc cagaatgctc tccggcagcg tcacggaagc ttcactgaga cacgctagcg
    16621 aactgatgaa taaatagccc atagagagga aacagtggat aaaaaagcaa agaaaagagt
    16681 gcgcggagta gctatctttc tggtatttct ggcaatggcg atcacaat
//
LOCUS       DAOSFN010000044        16668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2820_length_16668_cov_8.406369, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000044 DAOSFN010000000
VERSION     DAOSFN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2820_length_16668_cov_8.406369"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..397)
                     /locus_tag="PLO35_04360"
     CDS             complement(113..397)
                     /locus_tag="PLO35_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78931.1"
                     /translation="MAGKFFNRFIPAFIPLLFEVARHFRRDTSHNIDIKKFDKSQEKL
                     GTIEHLLVRLEKKMIQHRDNAQAFNNRITWWLALNSALLVAIAVKLFFLQ"
     gene            complement(412..1254)
                     /locus_tag="PLO35_04365"
     CDS             complement(412..1254)
                     /locus_tag="PLO35_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78932.1"
                     /translation="MKKIILITILIIQTALLLAVFDDYIPSARARAMGGAYTGVVDDV
                     SAVFFNPAGLAQVHNGVQVGFAQLNGQKFADYRTAAAGFKLPKKLGTIGLGARVFGVS
                     YEDMDLMSEQTFSLSHGFVLQHDIHSTISIGYTANYYRLSFEDEDPDDAIGFDFGATA
                     LLHGRTRLGFSVTNLGQAVMGDANQNNLPAKLALGISYQPYDQVTTSIEVKKDFAQET
                     EFMGGVEAQLLEPLSIRFGVHQNPATWNAGVGIDIKGIEIDLSYTTHAVLPGTLYGNL
                     GYKF"
     gene            complement(1269..2708)
                     /locus_tag="PLO35_04370"
     CDS             complement(1269..2708)
                     /locus_tag="PLO35_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78933.1"
                     /translation="MNKPLLLAVMLVLIAGLSAQVRTCYEIQYTDNPNGASPFNGQSV
                     TVQGIVVAEVFYTGTSTSNRGFVISDPEGGPWSGLLIFTNTYNPARGDLVQVTGTITE
                     YYDFTEMSPTTGFQVISQGNPMPQPTVITTDQLDNAATAEQYESVFVRVQDVTITAVP
                     NSYGEFYVNNSTAACQVDDQCFPRSGFSWPQMNVGQSWARVQGVVDFSFSYYGINPRD
                     MQDLVQLDTVANATVSVQTTNATLNTEVDVPIITSRLKPNWYISSYSATIAVDPNKII
                     FQGIKTSGTTITPMDPQVTVSPAGDLITVVYQSQEPISSSADDIPLFYLVMEPQVYGE
                     AIIDLQSFSYDTTPINSLYDGKVLTKINKDIAFLNIGRAGSAKNSFNPELNEKIVIEY
                     GCRAVATGINTRAIVRIYDAQGRLVATPVNKTISSSLGIETIQWDGRDTNMKLLPIGL
                     YYCHLEVIDRASGAKEVTVQPIVIKSNLK"
     gene            complement(2709..3263)
                     /gene="lspA"
                     /locus_tag="PLO35_04375"
     CDS             complement(2709..3263)
                     /gene="lspA"
                     /locus_tag="PLO35_04375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOH78934.1"
                     /translation="MKKLNPAPAWILMSVLIVLDQITKVLTRLYTDLGQSIPILPSLF
                     GDTFRFTHLQNTGAAFSMSLSNPAINRVFFITASILAIIFIIYLLRKATHRLQVWAFG
                     FVLGGAFGNLIDRVFIGGVTDFVDVNIPDMLGMTRFPVFNVADSAIFVGMTLLIIDMI
                     FLKDEPQAAPNLEGQTIELDNKEL"
     gene            complement(3270..3671)
                     /locus_tag="PLO35_04380"
     CDS             complement(3270..3671)
                     /locus_tag="PLO35_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOH78935.1"
                     /translation="MAAKKYSAEKLKYFEELIQKELKESIAYLDDINRDQSKGARESS
                     GDLSSYAFHQADQGSDTNLMEQNVMLMEQERDKVGLLNDALHRLFEGQYGNCEMCGEL
                     ISEARLEMLPHAKYCINCKEKMEDKKRRQRR"
     gene            complement(3771..4583)
                     /locus_tag="PLO35_04385"
     CDS             complement(3771..4583)
                     /locus_tag="PLO35_04385"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOH78936.1"
                     /translation="MSAGYRETAAWLKDRLPAQPVAGLILGTGLNDLADSLEQLARFP
                     YSEIPGFAQSTAPSHKGVLVLAVLGGKPVLVLQGRFHFYEGHSMDAVVFPTRVLACLG
                     VKTLLVTNASGSLRKELEPGALVQITDHINFMGRNPLVGENDENLGERFPSMNQPYDP
                     AFLDRCSAIAASEGITLHRGVYIAVSGPSLETRAECAAFAMWGADLVGMSTVPEVIAA
                     RHAGMRVATFSIVTNYSNLFHDEAHSQQEIRDNAAKAAAGLRLVLSSLVAEL"
     gene            complement(4586..5170)
                     /locus_tag="PLO35_04390"
     CDS             complement(4586..5170)
                     /locus_tag="PLO35_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOH78937.1"
                     /translation="MSLTPIDVRHQEFSGSMFGYSKKEVRAFLEQLASEMEDYQNRQE
                     RELQRREQMQYEVKQEAAQASSAVEDLKRREELISRTLVFAEKTKADIIANARKEAEN
                     ILHEAELKAKRAIQEAKQYLGAMEHQYVHLKEQKRQFLMQFKSELQTFMDRIGKDPLL
                     TKDTEQLLDTEFRQIKEEIAPVGSESHPAGGRQD"
     gene            complement(5188..5451)
                     /locus_tag="PLO35_04395"
     CDS             complement(5188..5451)
                     /locus_tag="PLO35_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOH78938.1"
                     /translation="MSYTPLQVLLLFLIRLLQIYNWLILGRVLMSWIIRDPNNRFYHF
                     LYSITEPLLGRIRNLMPSMGLDLSPIIAYFILNLVSGMLGALL"
     gene            complement(5456..6016)
                     /locus_tag="PLO35_04400"
     CDS             complement(5456..6016)
                     /locus_tag="PLO35_04400"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOH78939.1"
                     /translation="MNIINQKTGWIEVICGSMFSGKTEELIRRIRRAEYARQRVLVFK
                     PAIDDRYDANNIVSHSQMQAPSIPIGEAGEIYQYITDDVKIIAIDEAQFFDASIVGIV
                     NDLADRGLRVIVAGLDQDYKGNPFGSMPELLAVAEYVTKNLAICVKCGNPANRTQRTI
                     HKGEQILVGSTEAYEARCRNCHEILD"
     gene            complement(6475..7221)
                     /locus_tag="PLO35_04405"
     CDS             complement(6475..7221)
                     /locus_tag="PLO35_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH78940.1"
                     /translation="MPDFLSKNRVSELAALKQKKQRRAQGLCVLEGLRLITQLAADGI
                     LPKEVFLLDGEEALPALASVPAFICDQRAMERICDTEHPQGLAALYELPKSDFKGFRR
                     AFYLDGISDPGNLGTIFRLAMAFGIDAVLLSEGCADPASPKVIRSSLGAVYKVPYAQM
                     NPIALKLPGLKVVGTSAEGDTALHDFQPPARAPMLIVIGSEAHGVTPEIARRCEQTLR
                     IEMAPGMESLNAAVAAGIVAHHLWVESLTS"
     gene            complement(7395..9200)
                     /locus_tag="PLO35_04410"
     CDS             complement(7395..9200)
                     /locus_tag="PLO35_04410"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOH78941.1"
                     /translation="MSRPKSFPTRLICVDPRENLRQSAGNRSVFSICLAGLLLLLSLN
                     LSAQSSETWTVLVYIAGDNNLWQNAVQDINDMESANLPEELTLVVQSDLPASSPYPGG
                     QRRLIRQDSSPDITSPLIQSLGTINSGKAQTLNDFARWGFNQYPSTRKALFIWGHGNS
                     WFKEDSPKWICPDDDAQEVMHVWNGSLRYAFSGLPYLDILLFDACSMQSLEVLSEVKD
                     AAWRIIGSEELVPAAGFPYQDFLDWFNGVFNAEQICYGIVDAYIASYQPGGSQNPGGA
                     NLRVTCSAVRGNEMNTFLGVLQGFNLKYRERATELLSYREGCWEMNDGYNDVDIYEYL
                     RAVRAKTSDPEMADDADTVINYWYLTRVLEANLNLPLSVGGAAIWFPWHRQYFDSLWT
                     YYVELDFSVSRWISLLNLAYGPDETAPYTPQVLSAQQSLGSLLLRIKLNPDPDELTLK
                     VQIQTADSLQTLDYPLDWGAEEVTLRIPVSGAGSVTVQSYDRSFNLSGPASASFQFKP
                     LPLLLEVHPNPLLKNSGGILRWTIPTGVTGEGSLEIYNLRGQKVLQRSLGQLSPGEGA
                     ALPATWPEFMDLGRGLYLLRLKVGKITARAKLTIL"
     assembly_gap    9365..9464
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9465..10046)
                     /locus_tag="PLO35_04415"
     CDS             complement(<9465..10046)
                     /locus_tag="PLO35_04415"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOH78942.1"
                     /translation="MSYRVLAINPGSTSTKIAVYDGDRPVFEKVLRHEPEELAPYPTI
                     IDQYDFRRQLVMEAMRENQVPPSTLHAVVGRGGLVRPVCGGTWKITPQMLEDLKDTSR
                     WGRIHASNLGAFIADAISKELNIPAFIVDPVVVDEFDEIARISGIPEIERKSLLHALN
                     LRYIARLCAQELGKDFHQTNQIGVHMGGGISVAA"
     gene            complement(10454..12442)
                     /locus_tag="PLO35_04420"
     CDS             complement(10454..12442)
                     /locus_tag="PLO35_04420"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH78943.1"
                     /translation="MIKRLIGFVCVMAAICLRAGIVVPLPSGDVMRLYSGYSALVIGN
                     SDYKHFPRLKGVKKDIQDVAKLFTKLGIPCEIKENLNAEQMLAALYQFVELKGSDPDL
                     GLILYYAGHGYTEETYDGSSVGYIVPVDAPLYRKDKNLFRQRSISMNKIKEMATIIRS
                     KHVLMIFDSCFAGTVFRGDPALPTEIDEKVSQPVRQFITAGDENEEVPDVSIFKATLL
                     KALGDGLADLNQDGFVTANELGHYLKSEVVNYSKGAQHPQYGKLYEPNYNRGDFVFAL
                     ANPPRQKKLDPAPKNDPEPKVETQWLYGKLEIESNVDATLYLNGAEQSEILAGQRSSF
                     ELGVGDYTVELRTASSTQKKLATVRTDKLDRLSFEFDKSAAALNTPKKASQKQSTGPK
                     VDTARTTPPKEFIRVGGGSFKRGCEQGYQDSKPVRDITVNSFYIYAREVTQRQWKEVM
                     PNNPSAFQNDDKPVDSVSWFDAVDYCNRRSRKEGLNPCYVIDKAGSNGIGQNAEDQSE
                     WKVSVNWNANGYRLPTELEWEYAARGGNQSRQSDYSGVGALSQLAWFSQNSAALGKDH
                     PDYGTHPVGTKDPNELGLYDMSGNVCEWVWDWYDAGYYAKASSVDPRGPVSGTEKVLR
                     GGSWLDYGPVSCHVAYRYSAKPMVSFQTFGFRVARSMK"
     gene            complement(12649..13866)
                     /locus_tag="PLO35_04425"
     CDS             complement(12649..13866)
                     /locus_tag="PLO35_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH78944.1"
                     /translation="MKHALVLIWLFLATALFAWELNPYGPQGMDLLCASFEGDVPVFG
                     AQFNNYHGICLDITGQWYFHQHWMQFFPVKAVQQLTPGVYMCAFGDGSFSDGIYNYDV
                     DEDSWILNEWFMWPNFIVKHNPTGSFFVGERDGLFRSDDAVNWYRVNGLPVQQCDSFA
                     WWGSNMVADIGSAVYYSSDAGQTWLQSTMPLLEGFRFASNGTLFAKMDAGSDSDGLWR
                     SDDRGATWNLEFYTTGLAKIGPVLGGTLPLGWRIPNEAGNYMALYDLQGNLEQMVHTS
                     LDSPVRQLDIFPLVNTPAFFVINANGCFFITSFPVEDSDPVQTPPLADFSLSVSPNPA
                     GSQISVTAFKAFNKPDVTLYDLRGRKLMNLAASLNLDGSLSCALPDLPTAVYLLRVRD
                     GVNRYAARICVRD"
     assembly_gap    13979..14078
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14083..15867)
                     /locus_tag="PLO35_04430"
     CDS             complement(14083..15867)
                     /locus_tag="PLO35_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOH78945.1"
                     /translation="MAKTVNVWIDGHHLEVPDTMTIIEAADKHGLYIPRLCYHPDLPP
                     TANCGVCVVELAGNPTPKRACCTPVTEGMKISTNSKKLRSYRKTLVEMILSNHEVVCP
                     TCVANNKCELQTLANNLGVDPDALPNILVKKPVDNSSLSIIRDTNKCIACGRCITVCN
                     EVQTVYALTMTDRGIESHPDTAFANGMANSPCVNCGQCTVYCPTGALRERSEIDAVWE
                     AILDPEKHVIVQEAPSIRVSLGEEFGMPLGSVTPKKMYAALRKIGFDSVMDTNFTADL
                     TIIEEGTECVTKLKAGDKRPLITSCSPGWIKFMETYYPDLADCVSTAKSPMSMFGVLS
                     KTYYAQEQGIDPSKIVSVAIMPCTAKKFEARRPEMRDSGFQDTDYVLTTRELLRMIKE
                     AGVDFSNLKDEDADEGMSFYTGAGTIFGATGGVMEAAIRSAYFLVTNTELEDLDIKAV
                     RGLEGVKEAALQVPGFGELRVAVAHGLSNARKVMDQVREGLATKGESPWHFIEIMACP
                     GGCVGGGGQPYGNDIASRARRGLALYEEDRNLPVRKSHQNPEVTRIYERFLGEPNSPL
                     AHKLLHTHYFERSVNTGQIVKEVTHKHH"
     gene            complement(15930..16295)
                     /locus_tag="PLO35_04435"
     CDS             complement(15930..16295)
                     /locus_tag="PLO35_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOH78946.1"
                     /translation="MLHTDMEVYKLSLQLVKSVYEYCVSFPKEEVYGLASQMKRAAIS
                     VPSNIAEGCGRSSDKELCRFLDISMGSLSELGTHMELSQLLGFNSDEKHLQELKKTIM
                     RVRQMTLNLKKSVAQRAYA"
     gene            complement(16359..>16668)
                     /locus_tag="PLO35_04440"
     CDS             complement(16359..>16668)
                     /locus_tag="PLO35_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOH78947.1"
                     /translation="GAILVMNEHQSIVDMLWSILRFFRHESCGKCAPCRNGCQQLYKL
                     ITKIKKGEGSMDDVELMLTIAETMQATSFCALGQSPIMGIRSAIENFKDEFIALCVTR
                     "
BASE COUNT         3538 a         4361 c         4713 g         3856 t
ORIGIN      
        1 tcatcaattc atcaatttca agccatccac actcccccgg ccgccaataa ctccgaaaac
       61 aacaaggaca ggccttgcgg cctgtcccct ttgatgaaga attgctccgc gtttactgca
      121 aaaagaagag cttgaccgct atcgccacca gcagggcgct gttgagggcc agccaccagg
      181 tgatgcggtt gttgaaggcc tgggcgttgt cgcggtgctg gatcatcttc ttttcgaggc
      241 ggaccaggag gtgctcgatg gtgccgagct tttcctggga tttatcgaat tttttgatat
      301 cgatgttgtg gctggtgtct ctgcgaaagt ggcgcgccac ttcaaacagc agagggatga
      361 aggccgggat gaagcgattg aagaattttc cagccataag tggtttcctc gttagaattt
      421 ataacccagg ttgccataga gggtacctgg caggacagcg tgggtggtgt aggagaggtc
      481 gatctcgata cccttgatgt cgatgcccac tccggcgttc caggtggcgg ggttttgatg
      541 gacgccaaag cggatggaga gaggctccag cagttgggct tccacgccgc ccataaactc
      601 ggtttcctgg gcaaagtctt ttttgacttc gatgctggtg gtcacctgat cgtagggctg
      661 ataagagatc ccgagggcca gtttggccgg caggttgttt tgattggcat ctcccatcac
      721 ggcctggccg agattggtga cgctgaagcc cagacgggtg cgtccatgca gcagagcagt
      781 ggccccaaaa tcaaagccga tggcgtcgtc ggggtcctca tcttcaaagc tgaggcgata
      841 gtagttggcg gtatagccga tgctgatggt ggagtggata tcatgctgga gcacaaagcc
      901 atgggagagg ctgaaggtct gctcgctcat gaggtccatg tcttcataag agacgccgaa
      961 gacgcgggcg ccgagtccga tggtgcccag ttttttgggc agtttaaatc cagccgcggc
     1021 ggtgcggtaa tccgcgaatt tctgtccgtt gagctgagca aagcccacct ggacgccgtt
     1081 gtggacctgg gccaggccag ccggattgaa gaagacggca ctcacgtcgt ccaccacacc
     1141 ggtgtaagct cctcccatgg ccctggcgcg agcggagggg atataatcgt caaacacagc
     1201 cagcaggagc gccgtctgga tgatcaggat ggtgataagg atgatttttt tcatgctttg
     1261 ctccccgctt atttgagatt ggatttgatc acgatgggct gcacggtgac ctctttggcg
     1321 ccgctggccc ggtcgatcac ttccagatgg cagtaataga ggccgatagg caggagcttc
     1381 atattggtgt cgcgtccgtc ccactgaatg gtctcgatcc ccagggaact gctgatggtc
     1441 ttattgacgg gggtggcgac cagacggccc tgagcatcat agatgcggac gatggcgcgg
     1501 gtgttgatcc cagtggccac ggcccggcat ccgtattcaa tcacgatctt ctcgttgagc
     1561 tcggggttga aggagttttt ggcgctgccg gcgcgtccta tattgaggaa ggcgatgtcc
     1621 ttgttgatct tggtgagcac cttgccgtcg tagagagaat tgatgggagt ggtgtcgtag
     1681 gagaagctct ggaggtcgat gatggcttcg ccatagacct ggggctccat caccagatag
     1741 aagagcggga tatcgtccgc gctggaactg atgggctcct gagactgata gacgaccgtg
     1801 atcagatctc cggcagggga aactgtgacc tgcgggtcca tcggagtgat ggtagtgccg
     1861 gaggttttga tgccctgaaa gatgatcttg ttgggatcga cggctatggt ggcgctgtag
     1921 ctggagatat accagttggg cttgaggcgg gatgtgatga tggggacgtc aacttcggta
     1981 ttgagagtgg cgttggtggt ctggacggag acggtggcgt tggccacggt atccagctgg
     2041 accaggtcct gcatgtcgcg ggggttgatg ccgtaatagc tgaaggaaaa atccaccacg
     2101 ccctgaacgc gcgcccagct ttggccgacg ttcatctggg gccagctgaa gccgctgcgg
     2161 gggaagcact gatcgtccac ctggcaggcg gcggtgctgt tgttgacata aaactcgcca
     2221 tagctgttgg ggacggcggt gatggtgacg tcctgcaccc tcacaaacac gctttcatac
     2281 tgctccgcgg tggccgcgtt gtcaagctgg tcggtggtga tgacggtggg ctggggcatg
     2341 ggatttccct ggctgatcac ctgaaaaccc gtggtgggag acatctcggt gaagtcgtaa
     2401 tactccgtga tcgtccccgt cacctgaacg aggtctcccc tggcggggtt gtaggtattg
     2461 gtgaatatca gcagaccgct ccagggaccg ccctccggat cgctgatcac gaagccgcgg
     2521 ttgctggtgc tggtgccggt ataaaagacc tcggccacga cgatgccctg cacggtcacg
     2581 gattggccgt tgaagggtga agcgccgttg gggttgtcgg tgtactggat ctcatagcag
     2641 gtcctcacct gtgccgacag gcccgctatc agcacaagca tcactgccaa aagaaggggc
     2701 ttgttcatct ataactcctt gttgtctaat tctatcgtct gtccctcgag gtttggggcc
     2761 gcttgtggct cgtccttgag gaagatcatg tcaataatca aaagcgtcat gcccacaaaa
     2821 atcgcgctgt ccgccacgtt aaacacggga aagcgcgtca tgccaagcat atccgggatg
     2881 ttgacgtcca caaagtccgt caccccgccg ataaacacgc ggtctataag gttgccaaaa
     2941 gcgccgccca gcacgaatcc gaaggcccag acctgcagcc ggtgggtggc tttgcgcagt
     3001 aagtatataa taaaaatgat ggccaggatg gaggccgtga taaagaaaac gcggttgatg
     3061 gcaggattgg agagcgacat actgaaggcc gcgccggtgt tttgcagatg ggtaaagcgg
     3121 aaagtgtctc caaacaggga gggcaggatg ggaattgatt gtcccagatc ggtatagagc
     3181 cgcgtgagca ccttggtgat ttgatcgagt acgatcagga cgctcatcag gatccaggcc
     3241 ggggcgggat tgagtttttt caccagaatt taccttctct gccggcgctt tttgtcttcc
     3301 atcttttctt tgcagttgat gcagtacttg gcatggggga gcatttccag ccgggcttcc
     3361 gagatcagct ctccgcacat ttcacaattg ccatactggc cttcgaagag acggtgcagg
     3421 gcatcgttga ggaggccgac tttgtcgcgc tcctgctcca tcagcatcac gttttgctcc
     3481 atgaggttgg tgtcggaccc ctggtcagcc tggtgaaagg cgtagctgga gaggtcgccg
     3541 ctgctttcgc gggcgccttt gctttggtcc cggttgatgt catccagata ggctatactt
     3601 tctttgagct ctttctggat gagctcttca aagtatttca gcttttccgc ggagtatttc
     3661 ttggcggcca tatatcgctc ctatgaatta cacgtttttt gaggttttgt cattgctgtg
     3721 aaatgccctc aaactgtcaa ccaaaaagat agaggactcg gcacggcacg tcagagctcc
     3781 gccaccaggc tactcaggac cagtctgagg cccgcggcgg ctttggcggc attgtcgcga
     3841 atctcctgct gggaatgggc ttcgtcatgg aagaggttgc tgtagtttgt cacgatcgaa
     3901 aaagtggcca ctctcatgcc ggcatgccgg gcggcgatga cttccgggac ggtggacatg
     3961 cccaccagat cggcgcccca catggcaaaa gcggcacact cggcccgggt ttccaagctg
     4021 ggaccgctca cggcgatata gactccccgg tgaagggtga tcccttctga ggcggcaata
     4081 gcgctgcaac gatccagaaa ggcgggatcg tagggctgat tcatggatgg aaagcgttcg
     4141 ccgagattct cgtcgttttc tcccaccaga ggattgcggc ccataaagtt gatatggtcc
     4201 gtgatctgca ccaaagctcc cggttccagc tcttttctga ggcttccgga ggcgttggtc
     4261 accagcagag tttttacccc caggcaagcc agcacccggg tgggaaagac caccgcgtcc
     4321 atgctgtggc cctcataaaa gtggaagcgg ccttgcagga ccagcactgg tttgcctccc
     4381 agtacggcca ggaccagcac gcccttgtga gagggagcgg tgctctgagc gaagccggga
     4441 atctcggaat aaggaaagcg ggccaattgc tccaggctgt ctgccaggtc attcaagccg
     4501 gtgccgagga tcaatcccgc caccggttgg gccggcaaac ggtctttgag ccaggcggct
     4561 gtctctctgt agccggcgct catcgtcaat cctgtctgcc cccggccgga tgggattccg
     4621 accctacagg ggcgatctct tctttgattt ggcgaaattc ggtgtcgagc agctgttcgg
     4681 tgtctttggt gagcaggggg tctttgccga tgcggtccat aaaggtctgc agttcgctct
     4741 tgaactgcat gaggaactgg cgtttctgct ctttgagatg cacatactgg tgttccatgg
     4801 cgcccagata ctgcttggct tcctggatgg cgcgtttggc cttgagttcc gcttcgtgaa
     4861 ggatattttc agcttccttg cgggcgttgg cgatgatgtc cgccttggtc ttttcggcaa
     4921 agacgagggt gcggctgatc agctcttcgc gtcgtttgag atcttcgacc gcggaactgg
     4981 cctgagcggc ttcctgtttg acctcatact gcatctgctc gcggcgctgc agttcccgct
     5041 cctggcggtt ttgatagtct tccatttcag aggccagctg ctccagaaaa gcgcgcacct
     5101 ctttcttgct gtagccgaac atagagccgc taaactcctg atggcgtacg tctataggtg
     5161 tcagggacat ggtgttcctc ccttaattta taacagagcg cccagcatcc cggataccag
     5221 attcaggatg aaataggcaa tgatgggtga caaatccagg cccatggagg gcatgaggtt
     5281 acggatgcgg cccaaaaggg gctccgtgat gctgtagaga aagtggtaaa aacggttgtt
     5341 gggatcgcgg atgatccagc tcatcaaaac ccggcccagg atcagccagt tatagatctg
     5401 gagcagacga ataaggaaaa ggagcaggac ctggagtggg gtgtaactca agctcttagt
     5461 ccaggatctc gtggcagttg cggcaacggg cctcataggc ctcggtgctg cccaccagga
     5521 tctgctcgcc tttgtggatg gtgcgttgcg tgcggttggc gggattgccg catttgacgc
     5581 agatggccag gtttttggtc acatattccg ccacggccaa cagctctggc atgctgccaa
     5641 agggattgcc cttgtaatcc tgatccagac cggccacgat cactctcaga ccccggtccg
     5701 ccaggtcgtt gacaatgccc acgatgctgg catcaaagaa ctgggcctca tctatggcaa
     5761 tgatcttgac atcatccgtg atgtactgat aaatctcgcc cgcctcgcca atcggaatag
     5821 agggggcctg catctgggag tgggaaacga tgttgttggc gtcgtaacgg tcgtcgatgg
     5881 cgggcttgaa gaccagcact ctctgcctgg catattcggc ccggcggatc ctgcggatca
     5941 gttcctccgt cttgccactg aacatactgc cgcagataac ttctatccag ccagtctttt
     6001 ggtttataat attcactctc tctcccatga cttttgatgc ggattacaag gctggaaatc
     6061 ctgcctattt tgtcaaatac tatccgtgga gggggcggga aaagttacgg ggtatgcgtt
     6121 acgcgttatg cggtacggcc acgccccgaa ttggaattcg acgctaaggc tcacggaaac
     6181 gaggacgttc ccggctccac tattgagagc attctaagtc ctccgggtct ctctctgtat
     6241 cctccgtggc ctctggggtg aaaagaaaaa ctcggtatct ccgcagtcca tcccaaaaac
     6301 cttgaaaccc ctcatcactt accatcaatt catcaaatcc gtgcccttca ctctccctgg
     6361 cgttttgcag gctcctctca aattgtcaat tctctgtccc cccaaaaaac tctgttcctc
     6421 tgttttgaaa aacctctgct tttctcttgt caaacctctg ctttactctg tgttttatga
     6481 agttaaactc tcaacccata gatgatgcgc cacaatgcct gccgcgacgg ccgcgttgag
     6541 agattccatc ccgggggcca tttcgatcct gagggtctgc tcgcagcgtc tggctatttc
     6601 aggagtgacg ccatgtgctt cggaaccgat cacgatcagc atcggcgctc tggcgggagg
     6661 ctggaaatca tgcagggcag tgtcaccctc agcactggtg ccgacgactt tgaggcccgg
     6721 cagtttgagc gcgatgggat tcatctgggc atatggtacc ttgtaaaccg ctcccaggga
     6781 agatcttatc actttgggag aggcaggatc ggcacagcct tcagacagca aaacggcatc
     6841 gatcccaaag gccatagcca acctgaagat ggtgcccaga ttgccgggat cggaaatacc
     6901 gtccagataa aaagcccgcc gaaacccttt gaagtcgctc ttgggcagtt cataaagcgc
     6961 tgccaggccc tgaggatgtt cggtgtcgca gatcctctcc atagcgcgct ggtcacagat
     7021 aaaagccgga actgaagcca gcgccggcag cgcctcttcc ccatccagca aaaagacttc
     7081 tttgggcagg atgccgtctg cagcgagttg agtgatgagg cggagtcctt ccagcacgca
     7141 gagtccctgg gcccggcgct gttttttttg tttgagggca gccagttcag agacgcggtt
     7201 tttgctcagg aaatcaggca ttcacactcc tttttttaag ctatgaattt gcatatatct
     7261 acacacttat ccacaatggc ggtggataat cccgccagcg tatatagttg atattgatag
     7321 gcttacaatt tccatccaca aaggcctaaa agttatccac atcttatcca cattgccagc
     7381 aagcctctga tccactagag tatagtgagt ttggcccggg ctgtgatctt ccccactttg
     7441 aggcgcaaga gatacaatcc tctgcccagg tccataaact cgggccaggt ggctggcaag
     7501 gcagctcctt ctcccggaga aagctgtccc agagagcgtt gcagcacttt ttgccctctg
     7561 aggttgtaga tttccaggct gccctcaccc gtcactccgg tggggatcgt ccagcgcaga
     7621 atcccgccgc tgtttttcaa caggggattg gggtggactt ccagcagcag cggcaagggc
     7681 ttgaattgaa agctcgcgga ggccggtccg gagaggttga aggaccggtc atagctctgc
     7741 actgtgacgc tccccgcacc tgaaaccgga atccgcaggg tgacctcctc agccccccaa
     7801 tccagtggat aatccagggt ttgcaggctg tctgccgtct ggatttgcac cttcagagtc
     7861 agttcatccg gatcgggatt gagcttgatc ctcagcagca gagatcccag cgattgctga
     7921 gcgctgagca cctgaggagt atagggcgcg gtttcatcgg gtccgtaagc cagattgagc
     7981 aggctgatcc agcgactgac ggaaaaatcc agctccacgt aataggtcca gagcgagtca
     8041 aaatactgcc ggtgccaggg aaaccagatg gcggccccgc ctacgctgag gggcaggttc
     8101 agattggcct ccagcaccct ggtcagatac cagtaattga tcacggtgtc ggcatcgtcc
     8161 gccatttccg gatcagaggt ttttgccctc accgctctaa ggtattcata gatatccaca
     8221 tcgttgtagc cgtcattcat ctcccaacag ccttcccgat aggagagcag ttccgtggcc
     8281 cgctcgcggt atttgaggtt gaagccttgc agcaccccca aaaacgtgtt catctcattg
     8341 ccccgcaccg cggaacaggt aacccgcagg ttcgctccac cgggattttg actgccgccc
     8401 ggctggtagg aagcgatata ggcgtccacg attccatagc agatctgctc ggcattaaag
     8461 acaccattga accagtccag aaagtcctga tagggaaagc cggccgcggg gaccagttct
     8521 tccgagccaa taatcctcca ggcagcgtct ttcacctcgc tcagcacttc caggctttgc
     8581 atgctgcaag cgtcgaagag caggatgtcc aggtatggca gcccactaaa agcgtatctg
     8641 aggctgccgt tccagacgtg catcacttcc tgagcgtcgt catcgggaca gatccacttc
     8701 ggggaatcct ctttgaacca gctgttgccg tgtccccaga tgaacagcgc tttacgggtg
     8761 gagggatact gattgaagcc ccagcgggca aagtcgttga gagtctgtgc cttaccgctg
     8821 ttgatggtgc ccagggactg aatcagtggc gaggtgatat cgggtgagct atcctggcgg
     8881 atgaggcgtc tctggcctcc gggataggga ctggaggcgg gcaggtcgct ctgcaccacc
     8941 agggtaagtt cctctggcag attggcgctt tccatgtcgt tgatgtcctg aacggcgttt
     9001 tgccagaggt tgttgtcgcc agctatatag accagcaccg tccaggtctc ggagctttga
     9061 gcgctcagat tgagggataa aagcagcagc aggcccgcga ggcatatact gaaaacagat
     9121 ctgtttcccg ccgattgacg cagattttcc cgcggatcga cgcagattaa ccgggtagga
     9181 aaagatttag gtctcgacac ggataatcca ttcaatctgc gaaatctgcg tatctatccg
     9241 cgtgatctgc gggaaacagg actatggtca aattctaata ctccatcgcc gcttcgaggc
     9301 cttccaaaac ccgcacgccg cccatgctga gggcttccat ttccttttcg ccggggtaga
     9361 gcatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggccgc cacggagatg
     9481 ccgccaccca tgtgaacgcc gatctgattg gtctgatgaa aatccttgcc caattcctga
     9541 gcgcaaagcc tggcgatgta acgcaggttg agagcgtgca gcagggattt acgctcgatc
     9601 tcaggaatac cggaaatccg ggcaatctcg tcaaattcat ccaccaccac ggggtccacg
     9661 ataaaggctg gaatattcag ctctttggag atggcgtccg cgataaaagc tcccagattg
     9721 gaagcgtgga tgcggcccca gcggctggtg tccttgaggt cttccagcat ctgaggagtg
     9781 atcttccaag tgccgccaca gacgggacgg accaagccgc cacgaccgac cacggcatgc
     9841 agagtggaag gcgggacctg gttttcccgc atggcttcca tcaccaactg cctgcgaaaa
     9901 tcatactggt cgataatggt gggatagggg gccagctctt ccggctcgtg acgcaacacc
     9961 ttttcaaaaa ccggacggtc tccatcatac acggcaatct tggtggaggt ggaaccgggg
    10021 ttgatggcga gtacgcgata ggacataagt tctcctgtgg aagtatttta tattttttgg
    10081 catgaaagct agggggcagg acttttgtca atgataaatc aaggcgaatt gagagttgag
    10141 aattgtcatt ggggaggaca tctgccagaa acgctcgcta ttggggttgg aagcgtcttc
    10201 gtttccatgg gcccgctttg tcgatttcca actcggcggc gtgctctaac tactgagcta
    10261 cagaggtttc tttttcacta ctgagatacc gaggaaccag agagagctac agagaatgtt
    10321 ttgattttga atgggcttgc cagtggagcc gaattccaat ctaccgagtg acccgatctc
    10381 gcaaacgaga acgcttgcta ctctataaaa cctgcctgaa gacgggcact caagcccaat
    10441 gaactgaggc ggatcatttc atgctcctgg caactctgaa cccaaaagtt tggaaagata
    10501 ccatgggttt ggcgctgtag cgataggcca catgacagga aacgggcccg tagtctaacc
    10561 agctgccgcc tctcagcact ttctccgttc cggaaaccgg tccgcgtgga tcaacggacg
    10621 aagccttggc ataatagccg gcgtcatacc agtcccaaac ccattcgcag acgtttccgc
    10681 tcatatcata gagacccagt tcgttggggt cttttgttcc cacggggtgt gtcccgtagt
    10741 ccggatgatc tttgccaagc gcagcggaat tctggctgaa ccaggccagt tgggagaggg
    10801 cccccactcc gctgtaatcg ctttgcctgc tttggtttcc accccgggcg gcatactccc
    10861 attccagttc cgtgggcagg cgataaccgt tggcgttcca gttcacgctc actttccact
    10921 cgctctgatc ttcagcgttt tgtccgattc cgttagatcc ggccttgtcg atcacgtagc
    10981 agggattgag gccctccttg cggctgcgtc ggttgcagta atcgacggcg tcaaaccagg
    11041 agacgctatc cacgggttta tcatcgtttt gaaaggcgga ggggttgttg ggcatcactt
    11101 ccttccactg cctctgtgtg acctcgcgcg cgtagatata aaacgaattg acggtgatat
    11161 cgcggacggg tttggaatcc tgatatccct gctcacagcc ccttttgaag ctgccccctc
    11221 ccacccgaat gaattctttg ggtggagtgg tgcgggccgt gtccaccttg gggccggtgc
    11281 tttgtttttg ggacgctttt ttgggagtgt tcagcgcggc ggcgctttta tcaaactcaa
    11341 agctcagcct gtccagcttg tccgtcctga cggttgccag cttcttttgg gtggaagagg
    11401 ccgtcctcaa ctccactgtg tagtctccca cccccagttc aaaactggac ctctggccgg
    11461 cgaggatctc actctgctcc gccccattca aatagagcgt cgcgtccacg ttgctttcta
    11521 tttccagttt gccatatagc cactgggttt ccactttcgg ctcgggatca tttttggggg
    11581 cggggtcgag ctttttttgc ctgggaggat tggcaagggc gaagacaaaa tccccccggt
    11641 tgtaattggg ctcgtacagc tttccgtact ggggatgctg agcgcctttg gagtagttta
    11701 ccacctcact tttcagataa tgtcccagct cgttggcggt gacaaatccg tcctggttta
    11761 aatcggcaag accgtctccc agggctttca gcagagtagc cttaaagatg ctcacatcag
    11821 gcacttcctc attttcatcc ccggccgtga tgaactgacg gacgggctgg gatacctttt
    11881 cgtctatttc tgtaggcaaa gccggatcgc ctcgaaaaac cgttccggca aagcaactgt
    11941 caaagatcat gaggacgtgt ttggacctga tgatggtggc catttccttg atcttgttca
    12001 tggaaatgct gcgctggcgg aagagattct tgtccttgcg atacaaggga gcgtccaccg
    12061 gaacgatata acctaccgag gaaccgtcgt aggtctcttc cgtgtagccg tgacccgcgt
    12121 agtatagaat gaggcccaga tcgggatcgg agcctttgag ctccacaaac tggtataggg
    12181 cggccagcat ctgctccgcg ttgaggtttt cctttatctc acaggggata cccagcttgg
    12241 taaagagctt cgccacatcc tgaatgtctt tttttacgcc cttgagcctg gggaagtgtt
    12301 tgtaatcgct attccctatc accagggccg aatagcctga gtatagacgc atcacgtcgc
    12361 cactgggcaa agggaccaca atcccggccc gcagacagat ggcggccatg acacacacaa
    12421 aaccgatcag ccttttgatc atcgctttcc cccaaatctg tgatgatccc caaatatcaa
    12481 aacgcgcctt tcgtgtcaag ttaatatcgc tgcctgtcct atctcctcta ccacctcagc
    12541 tgctccccac gtgcactcct cctttaccta ggcaaaggag aggcataggt ggagtatagg
    12601 tggagtaaag gaggctaaag gcaaaggact gggagacaag ctcgtgcgct aatcccgcac
    12661 gcagatcctg gccgcgtacc gattcactcc gtcccgcacc ctgagcagat aaaccgctgt
    12721 gggaagatca ggtagagcgc aactgagcga gccatccaga ttgaggctgg ccgccagatt
    12781 catcagcttc ctgccccgca gatcatagag ggtgacatca ggcttattga aagccttgaa
    12841 agcagttacg gatatttgcg acccggcggg attgggagat accgagaggc tgaaatcggc
    12901 aagcggaggt gtttgaacgg gatctgaatc ctccaccgga aagctggtga tgaagaaaca
    12961 gccgttggca ttgatcacaa agaaggccgg agtgtttacc agcgggaaga tatccagctg
    13021 gcggacagga ctatccagcg aggtgtggac catctgctcc agattgccct gcagatcgta
    13081 gagggccatg tagttaccgg cttcgttggg gattctccat cccaggggga gagtgccacc
    13141 caggacggga ccgattttag cgaggccggt ggtgtagaac tccagattcc aggtagctcc
    13201 acggtcatca gacctccaga ggccgtcgga atcgctgccg gcatccattt tggcaaagag
    13261 ggtgccgttg gaagcgaagc ggaagccttc cagaaggggc atggtggatt gcagccaggt
    13321 ttgaccggca tcgctggagt agtataccgc ggatccgatg tccgccacca tgtttgagcc
    13381 ccaccaggca aagctgtcgc attgctgaac aggtaagccg ttcacccggt accaattaac
    13441 cgcatcatcc gatctgaaca gcccgtcccg ctctcccaca aaaaaactgc cggtgggatt
    13501 gtgtttgacg atgaaattgg gccacataaa ccactcgttc agaatccagg aatcctcatc
    13561 aacatcataa ttgtagatac cgtcgctgaa agagccgtcg ccaaaagcgc acatgtagac
    13621 gccgggggta agctgctgaa ctgctttcac agggaaaaac tgcatccagt gctgatggaa
    13681 ataccactgg ccggtgatat cgagacagat gccgtgatag ttgttgaact gggccccaaa
    13741 gacagggacg tcgccttcga agctggcgca aaggaggtcc atgccctgag gaccatatgg
    13801 gttgagctcc caggcaaaaa gcgcggtagc caggaatagc cagattaaaa cgagcgcgtg
    13861 tttcataagg aactccttga gataaagcct tatcgctgtc tgaatatgaa gcaatatttg
    13921 ttccgcaaac cagaagaagg gcagacgtcc tcgtctgcca aggcgcgatc agcacctgnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg gatcagtgat gtttgtgggt
    14101 gacttccttg acgatctggc cagtatttac cgaacgttca aagtagtggg tgtgcagcag
    14161 cttgtgggcc agcggggaat tgggctcgcc caggaagcgt tcatagatgc gggtgacttc
    14221 aggattctgg tgagatttgc gcaccggcag gttgcggtct tcctcataga gggccaaacc
    14281 gcgacgcgct ctggaagcga tatcgttgcc atagggttgt ccaccgccgc caacgcagcc
    14341 accgggacaa gccatgatct cgataaagtg ccagggtgat tcgcctttgg tggcgaggcc
    14401 ttcacgcacc tggtccatca ccttgcgggc attggaaagt ccgtgggcta cggccacgcg
    14461 cagttcgcca aaaccgggga cctgcagagc ggcttctttc acgccttcca gaccgcggac
    14521 ggccttgatg tccagatctt ccagctcggt gttggttacc aggaagtagg cggaacggat
    14581 agcggcttcc atcacgccac cggtggcgcc aaagatggtg cctgcaccgg tgtagaagct
    14641 catgccttca tcggcatcct cgtctttgag gttggaaaaa tccactccgg cttctttgat
    14701 catgcgcagc agctcacggg tggtgagcac gtagtctgta tcctggaaac cgctgtcgcg
    14761 catttcaggc ctgcgggcct caaatttctt ggcggtgcag ggcataatgg ccacgctcac
    14821 gatcttggag gggtcgatgc cctgttcctg ggcatagtag gtcttggaga gcacgccaaa
    14881 catgctcatg ggagatttgg cggtggagac gcaatccgcg agatcgggat agtaggtctc
    14941 catgaacttg atccagcctg gcgagcagga ggtgatgagg ggacgtttgt ctccggcttt
    15001 gagcttggtc acgcattcag tgccttcctc gatgatggtg aggtcggccg tgaagtttgt
    15061 gtccatcacg ctgtcgaagc cgatcttacg cagagcggca tacatcttct tgggggtcac
    15121 ggagcccagg ggcatcccaa attcctcacc cagagacacg cgaatggaag gagcttcctg
    15181 aacgatgaca tgcttttcgg gatccaggat ggcttcccag acggcatcga tctcgctgcg
    15241 ttcacgcaac gcgccagtgg gacaatagac ggtgcactga ccgcaattga cgcaggggct
    15301 gttcgccata ccattggcaa aagcggtgtc cggatgggac tcgataccgc gatcggtcat
    15361 ggtgagggca tagacggtct gcacttcgtt gcagacggtg atgcagcgtc cgcaagcgat
    15421 gcacttgttg gtatcgcgga tgatggagag agagctgttg tccaccggtt ttttgaccag
    15481 gatgttgggc agggcgtcgg ggtccacacc caggttgttg gccagggtct gaagctcgca
    15541 tttgttgttg gccacgcagg tggggcacac gacttcgtgg ttggaaagga tcatttccac
    15601 gagggtcttg cggtaggagc gcagtttttt ggagttggtg ctgatcttca tcccttcggt
    15661 gacgggagtg cagcaagcac gcttgggagt gggattgccg gccagttcaa ccacgcaaac
    15721 accgcagttg gcagtgggcg gcagatcggg atggtagcac aggcggggga tgtagagacc
    15781 gtgcttgtcc gcggcctcaa tgatggtcat ggtatccgga acttccagat gatgtccatc
    15841 tatccagacg ttgactgttt tagccataat agtctttaac ctctttatct tactattcgt
    15901 ttcataggtt atgcgttacg cgttacgcgt tatgcgtagg cgcgctgcgc cacagatttc
    15961 tttaagttca gagtcatttg tcgaactctc ataatagttt tctttaactc ttgcaagtgc
    16021 ttttcgtctg agttgaaacc cagcaactga gataactcca tgtgagtacc cagttcactc
    16081 aaagaaccca tagagatatc gagaaagcga cagagctcct tatcactgct tcttccgcat
    16141 ccttcagcga tattactggg aaccgatatg gcagccctct tcatctgaga agccagacca
    16201 tatacttctt ctttaggaaa cgagacgcaa tactcataca cgctttttac gagctgtaaa
    16261 ctcagcttgt aaacttccat atcagtatgc aacattgtgt ctccagttca cttcagtaat
    16321 tccgcttcac accttggaat acgcgtcccg cgtaacgctt aacgcgtaac gcacaaggcg
    16381 atgaattcat ctttgaagtt ctcgatcgcg gagcgaatac ccatgatggg agactggccg
    16441 agagcgcaga agctggtagc ctgcatggtt tccgcgatgg tgagcatgag ttccacatcg
    16501 tccatgctgc cttcgccttt cttgatcttg gtgatcagtt tatagagctg ctggcaaccg
    16561 ttgcggcagg gagcgcattt tccacaggat tcatgacgga agaaacgcag gatggaccac
    16621 agcatatcca cgatgctctg atgctcgttc atcaccagga tggcaccg
//
LOCUS       DAOSFN010000045        16528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2853_length_16528_cov_9.068840, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000045 DAOSFN010000000
VERSION     DAOSFN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 16528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16528
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2853_length_16528_cov_9.068840"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(410..3454)
                     /locus_tag="PLO35_04445"
     CDS             complement(410..3454)
                     /locus_tag="PLO35_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH78948.1"
                     /translation="MRRLALILTLLGLIAVSYAALVWPDAVPIRQGVNIEWFRTGIET
                     HDGGAIYVWSDTKLGERDLWAQKVDAQGNMVWGEPLLVDGKPDRQEDPVVTRTSDNYY
                     VIAWIDFSDDLDGSVYAQKVNADGQLMWQTGGVPVCVVPGVQLSLNMEPDNAGGVYIV
                     WMDSRNPSKDLFGQRLSATGAPVWSVNGIPIADGIGDEIQNTMLPDGQGGMMIGYTHT
                     YSSNEDIYVKRFLPNGTMAWTQPLALATGTGNQGKVRMASIGNSEFIYTWQDQTNDDP
                     DILAQKVNINGQMVWGQHAVVYGDSGTPNFTPQLNPRIVATSDNGAIIIWEDHRLDNQ
                     DPDLFAQKISSAGNVLWGAAGIQLCVAEFAQIGPRMSSDGAGGCYVVWDDLRNGNTPN
                     DDIYAQHLNASGTALWEVNGRAVCTAGNTQNGSLVKFSGNHVYVNWMDIRNGSVGIYY
                     QVLDSSGTPLLEQNGREVFWGLSGDTPLENYLILPRADDTAIIWQDTRFANLGYQIFF
                     QYLNPDGSVDLETNGRPVTASTGEHQVTPAAAVTPDGHIAVAWEDKRHDNPKIYLQLL
                     SPTGEKLWGDTGMELTQNSPLRQKDPFISYDQATNSFYVGWSNYDQVGTGFYYHVYGQ
                     RIQNGQKMWGPNGKIISDLATAELNNECVIFDMRDGYYVWQRYNPMDGTQSIYVKLVD
                     ANGDPLPGWPAEGLRASTHNSFDTVQMVPTSARTDQGLFVMWRDQREDWVSNYYGQHI
                     SATAQRLWDPLGVNLADYGREQEKPVILTDGRTDLITFAWCENINGMHDIIAHRYSLD
                     GNPLWGNLGYYVVQKDSSQTNPTFASFDNNGIAVAWTEYLALESDIYYKYINGDGTFA
                     GSPGGDIVCAAGKAQYEPKLTSMNNHAYAIWADGRSSGKTEILGLYAQKISNDTVAND
                     DPTAPPPAGFRLGQNHPNPFNPSTAIQFSTVGPDRHYSLEIYNLKGQRIKTLHEGKLP
                     AGNHSLIWNGKDANGQDVASGVYLYRLSDGSHFQSRKMLMMK"
     gene            complement(3557..4780)
                     /locus_tag="PLO35_04450"
     CDS             complement(3557..4780)
                     /locus_tag="PLO35_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH78949.1"
                     /translation="MPKIRTNLINPISPDRAEFLADHVIAYDDGVISALRPYDLTLDR
                     DAESALDCMALPGFIDLHVHLSQYRARGRFEPALLPWLEKHIFPAEALSAHTDYALEI
                     AKDFFRALFAAGTTTTVIYTAPFRQACQAAFEVAEELGARALIGMTLMDRNSPDALLH
                     DTSYAYENSVALRERWHDRSTLLDYIFTPRFAPTCSTELMKLIGEYASAQSAFIQSHL
                     SENKDEIAWVKELFGKASYTQVYADLGILGPHTIMGHAIHLSEEEIAILAETQTAIAH
                     CPDSNFFLKSGEFPYHRMWDAGLRIGLGSDVGAGTTLSMPHHAQMANYRQSACPLTPP
                     RLLWHLTMASAAILGWQDRIGSLELGKDADLCVFRIPEGHSLDAGLLSSLFFYGAEFK
                     TCRCIVRGRTVYSQD"
     gene            complement(4761..7448)
                     /locus_tag="PLO35_04455"
     CDS             complement(4761..7448)
                     /locus_tag="PLO35_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HOH78950.1"
                     /translation="MKAILLMISIIMLSTAGAALSLEELGFLINRTDQSTLQGYASQA
                     DSLVFAKETNETTLKLLLEFARRGDLAALQAKCLYRLSVGYHDLSAAVDWLRMETPTH
                     PILKYYMLNELELRFASQADSLSLVHAAGRIADSLFFAQVTKLESYNDYIEAFAKESV
                     DAFAVERSDSLALVMIDEFETAFPGSGYSHYSYYYKLYHSLKQSNTILADSLIARCGD
                     LSPAHAMISAQIIMSKTYRQAGEQSLERILPRVERYLALADSLRANNESIELLFNLYD
                     PEHWKNTLACEQLTLRYLKLKELASGYPEERFYFPPPELASLLAMADSIQFKDNDQGE
                     QARLEYLKAGLYALVKVPPYQEQAARHYLYCLIKGAPRKRFDQEALEALGKIHRELGI
                     TQNLSQWSRTLMNYNGIVFEDSTRALGLEDQRFTRIAIADYDNDGWLDLLFNGSRLYR
                     NQLGLEFCDLSDSLNLASRPGSGGIWADFNKDGWLDIASISHGPDGIGDALMKNHQGL
                     RFINVNERAGDIDDGFPSEAAAWIDVKMNGYPSLYVANYEKWMERKGFPDYFWLNEAG
                     YFSERGKELGFRTAADTDNPGLAGRGVAPADFNDDGRTDILVTNYRLNRNFLFVNTPD
                     GFVDQAFSTGIAGSYKQGYYGHSIGADWGDYDNDGDLDLFIANLAHPRYIDISDVSML
                     LRNDGPADYRIGDRTFSFHSFTDVTKTAGITWDELHSDPLWFDADNDGWLDLFITSVY
                     ENDRSYLYKNNGDGTFTDITWLSGARVYNGWGNACGDLNRDGLLDLVVGSGNGTRILY
                     NATPTVNEAIFVKPVWNGDAIQLVNEPRDYAQNPNSPAFGSRVRISLLHENGTSSSLV
                     RELNSARGTCSQNAPELHFGIGTATVTGIELIQYAQDQN"
     gene            complement(7451..8323)
                     /gene="hslO"
                     /locus_tag="PLO35_04460"
     CDS             complement(7451..8323)
                     /gene="hslO"
                     /locus_tag="PLO35_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOH78951.1"
                     /translation="MSDLLWRGMALSDSFRIFAVEATETAQRARDLHDLSPIATILMG
                     KMMCAAALLSLDLKSDNGEVSVRVDGDGPLEGGLVICDHYGQLRGYIRNPSLQLDAIQ
                     ANFLPGKQLGNGTLTVIKQLPGTSPWMGSTELVTGEIAEDLAHFYLQSEQVPSAVSLG
                     ILVDREAQIRACGGFVIQQLPFADPDKTEALIHNLNLTPNLSDLMDMGLGLQDIFDRF
                     VFKDMDYTLKPAHPVSYRCNCSKERFARSLLALGKDELQDLRDGIEPICHYCNSTYLF
                     SPEDIATLLKELED"
     gene            complement(8320..9792)
                     /locus_tag="PLO35_04465"
     CDS             complement(8320..9792)
                     /locus_tag="PLO35_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOH78952.1"
                     /translation="MKPLFLFRLVLFVTMLAIATQANAAELIDGQLWLEVFLDSGTQI
                     AMKSRDARTPVFTITEIGSPISKQVEGNLKLSFMNWDKLPFWGLLNRVEPLTAASDDG
                     DFATRQSFTWIDGKLALKKEKLIFEPDSFNTQATAQRYAEELGIPRSQVQAIPMLNAT
                     VKVLAGNNEHYFETPLRIKSDRDIQIGGLNLAFGGEFIIKAIGEKLVITHFLTLENYV
                     GGVIQNEIGSSAPLEALKTQAIAARTHAVSLLLYNRHQGDGYDLCNSTHCQVYKGQHL
                     SNANIITAVQATEHEVLISNGRIADATYHSACGGKTDSSANIWKGAPISHLMGVTCIE
                     AADSLDLSTESGARLWLDSKLNDPEASNWEKSSLQWKRDISRTRLASNLGLTKLSKIE
                     IIKRGHSGRILQMRFTGSKTVTLDSEYKIRQAFGGLPSSFFYIASSGSTIVVKGKGAG
                     HGVGMCQIGVLRLARSGWSYPDILSMYYPGTLISKDWMPQ"
     gene            complement(9800..11815)
                     /gene="metG"
                     /locus_tag="PLO35_04470"
     CDS             complement(9800..11815)
                     /gene="metG"
                     /locus_tag="PLO35_04470"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOH78953.1"
                     /translation="MKYLVTSALPYANGKLHVGHVAGAYLPADVFVRWLRLHNQDVIY
                     ICGTDEHGTPISISADAEGVTPDVVVKRYHDSIKAGFDAIDIQFDNFSGTARPPHHET
                     SRRFFLDLYEKGHIQARETRQFYCEHDKRYLPDRYVEGICPRCKATGARGDQCDACGQ
                     IYDTVTLIDPKCKICGNMPVVRDTKHWFIQLDDFREQLREWIAHKDYWKENVRNFMLN
                     LLDQGLIERSITRDLSWGIPVPLPEAEGKVLYVWFDAPIGYISSTVEWALNQGQPDRW
                     KDYWLDPETQLIHFIGKDNIIFHALIWPAMLMGQNQTYCLPHDIPANEFMNLEGQKIS
                     TSHNWAIWVDEFAKDFPGDFLRYYLAVNAPEKGDSDFSFKDFQLRINGELNNVLGNLA
                     NRVFAFSVKNFDGIIPPISYSAAARSVLEEAEALLKQIDEGFREYQVKKNTRLIMDIA
                     RLGNRWFDERKPWAAIKEDREHVLETLRVCADLLAMVSIALYPILPGSMNRLRQMMGL
                     PEAATWDDASLHNDLAITLHDTHPLFSKVTDEQVEAQLTKLQNAHTASKPACDPLKPS
                     ISYDDFSALDIRVAKVLEASRVPKTDKLLRLKVDIGSEVRELVAGIAESYEPEQLLGL
                     SVAMVVNLEPRKIRGIESRGMILAVHSEGTLTVVGPQDPCLPGSVIK"
     gene            complement(11812..12978)
                     /locus_tag="PLO35_04475"
     CDS             complement(11812..12978)
                     /locus_tag="PLO35_04475"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOH78954.1"
                     /translation="MAMIVKKFGGTSVGSIGLIKNIAEKIAAEKREQDSLVLVVSAMA
                     KTTDELWAMAHAVSARPNRREMDMLLTAGERISMSILSFALQECGLEAISFTGSQSGI
                     ITDDHHGNARILNVNAFRILSELEKGKIVIVAGFQGVSLQKEITTLGRGGSDTSAVAL
                     AGYLKADKCEIFTDVEGVYSADPRVVERPRLLASIDPDIMLTLAYGGSKVIHPRAVEF
                     ALKYGIPVEVKSSFSFQPGTMLKKNTETNMELRVINAIAHKEDLLRYLLPGSDVLTSL
                     LKSWSFEIFRYYLEADRLELIVEAKYESEIDYLLGSHGISPLSKETGIGFVIPVGVGI
                     NLDPGFLARILEICAGMELIRVNHTERSIELMMPSRQVKECVRLLHNAFIGEES"
     gene            complement(13116..15185)
                     /locus_tag="PLO35_04480"
     CDS             complement(13116..15185)
                     /locus_tag="PLO35_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH78955.1"
                     /translation="MKRILLYAISVLLLLAGCMNKEFTNPVDPEHIQSNLLAPYGLQY
                     QLSGESIRLNWTDRCNIEAGYKIDRRVNGGSWQTGYKSVGKNSTFCWDHSITQQGSYE
                     YRVYPFRESYNGSHAQISLNMVLNTAAPSFSHLSGTYNNSFGLWIHSTTPGAQIRYTV
                     NGPTPNASSPLYTPPYNVTQTNATIKAIAVCAGRTPSTVVTHSYILKPLNPSFSLAEG
                     TYETAQTCTLTCATAGATIHYTTNGSIPSSSSPVYGSPINFSQSTTIKAIAVKSGFSN
                     SDVISKTYTISTTVATPVIQKLTGVYNNTFTTTISCSTANSTIRFTLDGTEPSTSSTL
                     YTGALTISNICTLKAKAWKTGLTPSEVATASYTMRAANPNFNIAQGTYQVSVNLAIGS
                     ATTNAAIRYTTDGSNPTPSSTLYTGQINISKPTIIKAQAHYGSWEPSAVIRATYMFDE
                     YVAGGTFNNGTGNMTLSSFYIGKTEITQALYQNIMGSNPSSGAGIGVFHPVYNVSWFK
                     AIEFCNRLSSHFGLSPCYKYNNVEDPDLWPSGWDTNSGNHIYLSCNWNASGFRLPTEA
                     EWHFAARGGNSSHNYTYSGSNDVAYVAYYNNNSLNSTWDVASKPVNEVDTHLMSGNVR
                     EWCWDIYAAYPTGSYTNYTGATSGSSRCYRGGGYSDTPSLCTVIYRSSITPTTNAFMG
                     FRVARKY"
     gene            complement(15188..>16528)
                     /locus_tag="PLO35_04485"
     CDS             complement(15188..>16528)
                     /locus_tag="PLO35_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOH78956.1"
                     /translation="YGKIANIALDKGDFVFALKQDAVPITPQQTRPPAYVPPPSLELE
                     TRAGAFRMTADDSGSVYVDGVFVETITAILQISKRDMAPGVHQVDFVGSKGILSRQVG
                     VSIGETIDIHFSFRDVGSSLYKLWTELSCDSSASINISGFPDNITFGNYIFASDTDKT
                     YSITISKPDHVTQKMDIRPDVSGKKIKTKLFSNYCGITVTSEPSGAVVSLNGMKLGVT
                     PLTVDPLIGRYPPGEYLFSVELKDYFPVSKSAKLSNSIDNNVFVKLDKISSGQFKIKK
                     IDPEISVTLVDDRSKSEIKYKNSLTLYSGGYVLQADRWGYIPRSFKVEVKPNRLSEMP
                     ELKLDPIDKAMLRKLGAWKLYRNSSVLAILGTTAAAAVTYYFAENNYNDYMNTMSPSE
                     AQSFRDRYNQLKKYYYVTLGADVFFLANYIFSATKTSSTYKSIDRAYRQYKLGE"
BASE COUNT         3708 a         4198 c         4569 g         4053 t
ORIGIN      
        1 cccgcaaact cgttgtcatg catagcttta tcatggccgc ttgaactcca acgggtcgat
       61 aaactccacc ctctttctcc aatccctccc ataacttgcc gttgagatgc cgtgcgaccg
      121 gaggcgattt tcggcggaga gcccacggca tcgcgaggac ggctaagcgg caaggataaa
      181 cgggcggcaa atcattcagc aagataggta tccgatttcg cccttaccgg tggaacggat
      241 gcctgccctg atatagccca aggcatcccc aatgttatag gggatgggta gggggagctc
      301 cgattggcca aacgggagat gaataagggc gcgcttaccg tgtcttcact ccaaacagaa
      361 agcgccggca gtcaaaccac cagcgctatt cttatgtgag gtgtgatatt tatttcatca
      421 tcagcatctt acggctctgg aaatggctgc cgtctgaaag gcggtaaaga taaacaccag
      481 aggccacatc ctggccgttg gcgtcctttc cgttccagat caggctatgg ttcccggctg
      541 gaagttttcc ctcatggagg gtcttgatcc gctggccttt aaggttgtag atttcgaggc
      601 tataatgccg gtcgggacct acggtgctga attggatggc cgtgcttgga ttgaaggggt
      661 tgggatgatt ctgtcccagt ctgaatcccg cgggtggagg cgcggtggga tcatcgttgg
      721 ccaccgtatc gttggagatt ttctgcgcgt acaagcccag aatctcggtt tttccactgg
      781 aacgtccgtc cgcccaaatc gcgtaggcgt ggttgttcat gctggtcagt ttgggctcat
      841 actgagcttt accagcggcg cagacgatat ctcctccagg gctgcctgcg aaggtgccat
      901 cgccattgat gtatttgtaa tagatatcgg attcgagggc cagatattct gtccaggcca
      961 cggcgatgcc attgttgtca aaggacgcga aagtgggatt ggtctgcgag ctgtctttct
     1021 gcaccacata atagccaagg ttgccccaga ggggatttcc atccaggctg tagcgatggg
     1081 cgatgatgtc gtgcatgccg ttgatgtttt cgcaccaggc gaaggtgatc aggtcggtgc
     1141 gtccatcggt gaggatgacg ggtttttcct gctcgcggcc ataatcagcc aggtttacac
     1201 ccagggggtc ccaaagcctt tgcgcggtgg cggaaatgtg ctgtccgtaa tagtttgaca
     1261 cccagtcttc acgttgatct ctccacatca caaagagccc ctgatctgtc ctggcagagg
     1321 tgggaaccat ctgtacggta tcgaaactat tatgagtgga agctcttaag ccttccgcag
     1381 gccagcctgg cagaggatct ccattggcgt caaccagctt cacgtagatg ctctgggtgc
     1441 cgtccatggg attgtagcgc tgccagacat agtagccgtc gcgcatgtca aagatcacgc
     1501 actcgttgtt gagttcggca gtggcaagat cggagatgat cttgccgttg ggtccccaca
     1561 tcttctggcc gttttgaatt ctctggccat agacatgata atagaagcct gtgcccacct
     1621 gatcatagtt tgaccagccg acgtagaagc tgttggtggc ctggtcgtag ctgatgaaag
     1681 gatctttctg gcggagggga gaattctgcg tgagttccat gcctgtatcg ccccagagct
     1741 tttccccggt gggagacaag agctgcagat agattttggg gttgtcgtgg cgtttatctt
     1801 cccaggctac agcaatatgg ccatcaggag tgacagcggc ggccggagta acctgatgct
     1861 ctccagtgga agcagtcacg gggcgaccgt tggtctcgag gtcaacgctt ccatcgggat
     1921 tgaggtattg gaagaagatc tgatagccga ggttggcgaa acgggtatcc tgccagatga
     1981 tagcggtatc atctgccctg ggcagaatca ggtagttttc cagaggagtg tcgccactga
     2041 ggccccagaa aacttcccgg ccattctgtt ccagaagagg ggtgccggat gaatctaaaa
     2101 cctgataata gatgcccacg ctgccgttgc ggatatccat ccaattcaca tacacgtggt
     2161 taccagagaa cttgaccagg ctgccgtttt gagtgtttcc agcggtgcaa actgccctgc
     2221 cgtttacttc ccacagggcc gtcccggagg cattgagatg ctgggcgtaa atatcgtcgt
     2281 tgggggtgtt gccgttgcgc agatcatccc agaccacata gcagccgccc gctccgtcgg
     2341 aagacatacg aggcccgatc tgagcaaact cggccacgca caactggatg cccgcggctc
     2401 cccaaaggac attgcccgcg gagctgattt tctgggcaaa aagatcgggg tcctgattgt
     2461 cgagccggtg atcttcccag ataataatag ccccgttgtc actggtggca acgatgcggg
     2521 ggtttaactg aggggtgaaa ttgggggtcc cggaatctcc atagacgacc gcgtgctgac
     2581 cccaaaccat ttgcccgttg atgttcactt tttgggccag gatgtcggga tcgtcattgg
     2641 tctgatcttg ccaggtgtag atgaactctg agttgccaat cgaggccatg cgtactttgc
     2701 cctggttgcc agtcccggta gccaacgcga gaggctgcgt ccaggccatg gtgccattgg
     2761 gcaaaaagcg ctttacataa atatcttcat tactggaata ggtgtgagtg tagccaatca
     2821 tcatgccacc ttgcccatca ggaagcatgg tgttttggat ttcgtctcca atcccgtccg
     2881 cgatggggat gccatttaca gaccagaccg gggccccggt tgcagagagg cgctggccaa
     2941 agaggtcttt gctgggattg cgggaatcca tccacacgat gtaaacgccg cccgcgttat
     3001 caggttccat attgagcgaa agctgaactc ccgggaccac gcagacaggt actcccccgg
     3061 tttgccacat cagctggcca tcggcattta ccttctgggc atacacgctg ccatccaggt
     3121 catcgctaaa atcaatccag gcgatcacat agtagttgtc gctggtacgc gtgacgacgg
     3181 gatcttcctg ccggtcaggc ttaccatcca ccaggagggg ttcaccccac accatgttac
     3241 cctgagcgtc cactttctga gcccagagat cacgctcgcc aagtttggta tccgaccata
     3301 catagatcgc tcctccatca tgagtttcga tcccggtacg gaaccattct atgttcacac
     3361 cctgacggat gggaaccgcg tctggccata ccaaagcggc atatgaaacc gcgatcaggc
     3421 caagtagggt cagtatcaac gcgagtcttc tcacgtttcc tccttatttt ctattgattt
     3481 attagtttta ccggaactgg agataccagt tccgcccttc atggatgcaa taatccttcc
     3541 aaagcgggag cagtgtttaa tcctgggagt aaactgtcct gcccctgact atgcagcggc
     3601 aggtcttaaa ctccgctcca tagaaaaaca gactcgataa aagcccggca tctaagctgt
     3661 gtccttcagg tatcctgaac acgcacaaat cagcgtcttt cccaagctcc aacgacccga
     3721 ttctgtcctg ccagcccaaa atcgcagcgg aggccatggt caaatgccac aagaggcgtg
     3781 gaggcgtgag gggacaggca gattggcggt agttggccat ctgggcatga tgtggcatgc
     3841 tgagcgtggt tccagccccg acgtcgcttc ccaagccgat tctgagccca gcgtcccaca
     3901 ttctatggta gggaaactcg ccactcttga ggaagaaatt ggaatccggg cagtgggcaa
     3961 tggccgtctg agtttctgcc agaatcgcga tttcctcttc cgagaggtgg atggcgtggc
     4021 ccatgatggt gtgggggcca aggattccca gatcggcata gacctgggtg taggaagctt
     4081 tgccaaacag ctccttaacc caggcgattt catctttgtt ctctgataag tggctctgga
     4141 taaaggcact ttgagcagag gcgtattcac cgatgagctt cataagttca gtggaacagg
     4201 tgggggcgaa gcgaggggtg aatatatagt ccagcagggt tgagcggtca tgccatcgct
     4261 cgcggagggc cacgctgttc tcataggcat aggaagtatc atgcagcaag gcatccgggc
     4321 tgttgcggtc catgagggtc atgccgatca aagctctcgc tcccagctct tccgcgactt
     4381 caaaggccgc ttggcaggcc tgccggaatg gggcagtgta gatcacggtg gtagtggtgc
     4441 cggcggcaaa gagagcccga aaaaagtcct tagcgatctc cagagcgtaa tccgtgtggg
     4501 ctgaaagggc ctcagcagga aatatgtgct tttccagcca gggcagaagc gcaggttcaa
     4561 agcgccccct tgcccggtat tgggaaaggt ggacgtgaag atcgataaac ccaggcaacg
     4621 ccatgcaatc gagcgcgctc tcagcgtccc ggtctagagt gaggtcataa ggtctgaggg
     4681 ctgatatgac accatcatcg taagcgatca cgtgatcagc cagaaactcg gcccgatcag
     4741 gtgatatggg attgatgagg ttagttctga tcttgggcat attgtatcaa ttcaatcccg
     4801 gttactgtcg ctgtcccgat gccaaaatga agctccggag cgttttgcga gcatgttccc
     4861 ctggcggagt tgagttcccg gaccaaagaa ctggaagtcc cgttttcatg caggagtgag
     4921 atcctgactc tgctgccaaa agcagggctg ttggggtttt gagcataatc acgcggctcg
     4981 tttacgagct ggatggcgtc tccattccaa accggcttta caaagatcgc ttcattgacg
     5041 gtgggagtgg cgttgtagag aatcctggtg ccgtttccgc tgcctaccac gaggtccaaa
     5101 aggccgtcac ggttgagatc tccacaggca ttgccccaac cgttgtaaac tcgggcccca
     5161 gaaagccagg tgatgtctgt gaaagtgcca tcgccgttgt ttttatatag atagctacga
     5221 tcattctcat acacggaagt gatgaagagg tccagccagc catcgttgtc ggcatcgaac
     5281 cagagaggat cggagtgcag ttcgtcccat gtgatccccg ccgtttttgt gacatcggta
     5341 aagctgtgaa agctaaaagt gcggtcgccg atgcgatagt ccgcgggacc atcgttgcgg
     5401 agcagcatgc tgacatcgga tatatctata tagcggggat gagcaagatt ggcgatgaag
     5461 agatcgaggt ctccgtcgtt atcataatct ccccagtcgg ccccaatcga atgaccatag
     5521 tatccctgtt tatagcttcc ggcaatcccc gtgctaaaag cttggtccac aaagccgtcc
     5581 ggggtgttca caaagagaaa attgcggttt aggcggtagt ttgtgaccag aatgtcggtg
     5641 cgtccatcgt cattgaaatc ggcaggggcc actcctctgc ctgccaggcc aggattgtct
     5701 gtatccgcgg ctgttctaaa accgagttcc tttccccgtt ctgagaaata acctgcctca
     5761 ttcagccaga aataatctgg gaatcccttt ctctccatcc atttctcgta attggccaca
     5821 tataaagagg ggtagccgtt catcttcaca tcaatccagg ctgcggcttc ggaagggaag
     5881 ccgtcatcga tatctccggc ccgttcattg acattgataa agcgaaggcc ctgatggttt
     5941 ttcatgagcg cgtcgccgat accgtccggg ccgtgggaaa tgctggcaat atccagccac
     6001 ccgtctttgt tgaagtccgc ccagatgcct ccgctgcctg gacgtgaggc cagattgagg
     6061 ctgtcgctga ggtcacaaaa ctccagaccg agctgattgc gatagagacg cgaaccgttg
     6121 aagagcaggt ccaaccagcc atcgttgtca taatccgcga tcgcaatcct ggtaaaccgc
     6181 tggtcctcga gtcccagggc acgggtagag tcttcaaaca cgatcccgtt gtagttcatc
     6241 aaagttctgc tccactgcga gaggttttgg gtgattccaa gctcccgatg gatcttccca
     6301 agggcctcaa gagcttcctg gtcaaaacgt ttgcggggag cccctttgat caggcagtac
     6361 agatagtgac gagcggcttg ttcctggtag ggaggcactt tgacgagcgc gtagaggcca
     6421 gctttcaaat actcgagcct ggcctgttcg ccctgatcgt tgtccttaaa ctggatagaa
     6481 tcagccatcg caagcaagga agcaagttcc ggagggggga aatagaaccg ctcttcagga
     6541 tagccagaag ccagctcttt cagcttcaaa tagcgcagcg tcagctgttc gcaggcgagg
     6601 gtgtttttcc agtgctccgg atcatagaga ttgaacagca attcgatgga ttcattattc
     6661 gcccgcaggg aatccgcgag tgccagatac ctttccactc tggggaggat tcgttccaaa
     6721 gattgctctc ctgcctggcg atatgtcttg ctcatgatga tctgagctga gatcatagcg
     6781 tgggcgggac tcaggtcacc acatcgggcg atgagactgt ccgcaaggat tgtatttgat
     6841 tgcttgagag aatgatagag cttgtagtaa taggaatagt gactgtagcc cgagccggga
     6901 aaggcagtct caaattcgtc tatcatgacc agggccaggg aatccgatct ctccaccgcg
     6961 aaggcatcaa cgctttcctt ggcgaaggct tctatatagt cgttatagct ctcaagcttg
     7021 gtgacttgag cgaagaaaag gctgtccgcg attctgccag ccgcgtggac caggctgaga
     7081 ctatcggcct gagaagcaaa gcgcaactcc aactcattga gcatatagta tttgaggatg
     7141 gggtgagtgg gcgtttccat cctgagccag tctacagcag cgctgagatc atggtatccc
     7201 acggacagcc tgtagaggca tttggcctgc agggcagcga gatctcccct ccgggcaaat
     7261 tccaacagca atttcagagt ggtctcgttg gtttccttcg cgaagacgag tgaatccgct
     7321 tgagaagcgt atccttgcag ggtgctctga tccgtgcggt tgatcagaaa ccccagttct
     7381 tccaagctga gagcggcccc agctgtcgat agcatgatga tgctgatcat cagcaaaatg
     7441 gctttcatag ctaatcctcc agttccttta acaaggtggc aatatcctcc gggctaaaca
     7501 ggtaagtgct attgcagtaa tgacagatgg gctcgatgcc gtctctcaga tcctgaagct
     7561 catcctttcc caaagccagc aaggacctgg caaaacgttc cttggaacaa ttgcagcggt
     7621 agctgacagg gtgagcgggc ttgagggtgt aatccatatc cttaaataca aaacggtcga
     7681 agatatcctg caggcccaaa cccatatcca tcagatctga gaggttgggt gtgagattca
     7741 gattgtggat cagcgcttcc gtcttgtcag gatctgcaaa aggcagctgt tgaatcacaa
     7801 atcctccgca agccctgatc tgggcttccc ggtccaccag aattcccagc gataccgcgc
     7861 tgggcacctg ttccgactgc agatagaaat gggcgaggtc ttccgcgatc tcaccggtga
     7921 ccagttcggt ggaacccatc caaggagagg tgcccggcag ttgtttgatc acagtcaggg
     7981 tgccgttgcc caactgcttg ccggggagaa agttggcttg gatggcatca agctggagag
     8041 aggggttgcg gatataccct ctcaattggc cataatggtc gcagattacc aaaccgcctt
     8101 ccagaggacc gtctccgtct actctgacac tcacttcgcc gttgtcgctt tttaggtcaa
     8161 gactcaggag cgccgcggca cacatcatct tgcccatcag gatggtggcg attggagaca
     8221 ggtcgtgcag atcacgcgct cgctgagccg tttcagtggc ttccacggca aagatcctga
     8281 agctgtcgct gagggccatt ccgcgccaga gaagatcact cactgtggca tccagtcttt
     8341 gctgataaga gtgccgggat agtacatgga gagaatgtct ggatagctcc atcccgatct
     8401 tgccaggcgt aaaactccaa tttggcacat acctactccg tgaccagcac ctttgccttt
     8461 aaccacgatt gtggagccgg aactggctat ataaaaaaag gaagacggca agccgccaaa
     8521 ggcctgacgg attttgtact ctgagtccag agtgacagtt ttgctgcctg tgaagcgcat
     8581 ttgtagaatc ctgccggaat ggcctctttt gattatctcg attttgctga gctttgtgag
     8641 cccgagattg gatgcgaggc gggtcctgga gatatccctt ttccactgca gggaggattt
     8701 ttcccagttg ctggcttccg gatcattaag tttggagtcc agccacagcc gcgctccgct
     8761 ttcagtgctt agatcaaggc tgtccgcagc ttctatgcag gtgactccca tcaaatgtga
     8821 gatcggcgcg cctttccaga tattggcaga ggagtcggtt ttaccaccgc aagcgctgtg
     8881 ataggtggca tcagcgattc tgccgttgct gatcaagact tcatgttccg tggcctggac
     8941 ggcggtgata atgttggcgt tggaaagatg ctgaccctta tatacctggc agtgggtgct
     9001 gttgcagagg tcatagccat cgccctggtg acggttgtag agcagcaggc tgacagcgtg
     9061 ggtgcgggca gcgattgcct gagtttttag cgcttcaagg ggagcgctgc tgccaatctc
     9121 gttttggatg acgccgccga cgtagttttc caaggtgaga aaatgagtga tgaccagctt
     9181 ttcaccgatc gctttgatga taaactctcc tccaaaggcc agattaagtc cgccgatctg
     9241 aatatcccta tcgctcttga tcctgagggg agtctcgaaa tagtgctcgt tattgccagc
     9301 caggacttta actgtggcgt tgagcatagg gatcgcctgc acctgagatc tgggaattcc
     9361 aagctcttcg gcatagcgtt gcgcagtggc ctgggtatta aaggaatctg gctcaaagat
     9421 aagcttttcc ttcttcaaag cgagcttacc gtctatccag gtaaagctct gccgcgtggc
     9481 aaaatctccg tcatcgctgg cggctgtcag aggttcgact ctgttcaaca gaccccaaaa
     9541 gggtagtttg tcccaattca tgaaactgag tttgagattg ccctccacct gtttggagat
     9601 gggagaaccg atctctgtga tcgtgaagac aggcgttctg gcgtcccggc ttttcattgc
     9661 tatctgagtg ccgctgtcca gaaaaacctc caaccacaac tgcccatcga tgagctccgc
     9721 ggcattggcc tgggtggcaa tcgccagcat tgtcacaaac aggaccaggc ggaacagaaa
     9781 gaggggtttc atcgctacct tacttgatca ccgaacctgg cagacaagga tcctggggcc
     9841 ccacaacggt gagagtgcct tcagagtgga ctgccaggat catcccgcgg gattcgatgc
     9901 ctctgatctt gcgaggttcc agattgacca ccatggccac cgataggcca agcaactgct
     9961 ccggctcgta ggattccgcg atgccggcca ccagttccct gacctcgctt ccgatatcca
    10021 ctttcagacg cagaagcttg tcggttttgg ggaccctgga agcttccagg actttggcta
    10081 ctctgatatc caaagcactg aaatcgtcat aactgatgga gggcttgaga gggtcgcacg
    10141 cgggctttga ggccgtgtga gcgttttgca gttttgtcag ttgggcttcc acctgctcgt
    10201 ctgtgacctt gctgaagagg ggatgggtgt catgcagagt gattgccaaa tcattgtgca
    10261 aactggcatc atcccatgtt gccgcctcag gcagtcccat catctgtctc agtctgttca
    10321 tcgatcctgg caggatggga tacaacgcga tgctgaccat agcaagcaga tcggcacata
    10381 ctcgcagagt ctcaagcacg tgttctctgt cttctttgat ggcggcccag ggtttccgct
    10441 cgtcaaacca gcggtttcca agcctggcga tatccatgat caggcgggtg tttttcttca
    10501 cctgatactc ccggaagcct tcatctattt gcttcagcag ggcttcagcc tcctccagaa
    10561 cggatcgcgc ggctgcggaa tagctaatgg ggggtatgat accatcgaag ttcttgacgc
    10621 tgaaagcgaa aacgcggttg gccagattgc ccagcacgtt gttgagctcg ccgttgatcc
    10681 ttagctggaa gtctttgaag gagaaatcac tgtctccttt ttcaggagcg ttgacggcca
    10741 gatagtatct cagaaagtca ccgggaaaat ctttggcaaa ctcgtccacc cagatagccc
    10801 agttgtggct ggtggagatc ttttgccctt ccaggttcat aaattcgttg gccgggatgt
    10861 cgtgaggaag gcagtatgtc tgattttgtc ccatcagcat ggctggccag atcaaggcat
    10921 gaaagatgat attgtctttg ccgataaagt ggatcagttg agtctcggga tccagccaat
    10981 agtccttcca gcgatcaggt tgaccctgat tgagggccca ttccaccgtg gatgagatat
    11041 agccaatagg ggcgtcaaac cacacataga gcacttttcc ctctgcttca ggcaagggga
    11101 cgggaattcc ccaactgagg tcgcgggtga tgctgcgctc tataagcccc tggtccaata
    11161 ggttaagcat aaaattgcgg acgttttctt tccaataatc tttgtgagct atccactctc
    11221 tgagctgctc ccgaaagtca tccagctgga taaaccagtg tttggtatca cggacaaccg
    11281 gcatgttgcc acagatcttg cacttgggat cgatgagggt gaccgtgtca tagatttgac
    11341 cgcaggcgtc gcactggtct cctctggcac cggtggcttt gcagcgtggg cagatgccct
    11401 ccacgtagcg gtccgggaga tagcgtttgt cgtgctcgca atagaattgc cgggtctcac
    11461 gcgcctggat gtgccccttt tcatagaggt ccagaaagaa gcgtcgcgag gtctcgtgat
    11521 gaggaggacg ggcagtaccg gagaaattgt caaactggat gtcgatagcg tcaaatcctg
    11581 ctttgatgct gtcgtgatag cgcttgacca ccacatctgg agtcacgcct tccgcgtccg
    11641 ctgatatgga aatcggagtg ccgtgctcat cggtcccaca gatatagatc acatcctggt
    11701 tgtgaagacg cagccagcgg acaaagacat cggcaggcaa ataagcacct gccacgtggc
    11761 cgacatggag tttgccattg gcgtagggca aagcgctggt caccagatat ttcatgactc
    11821 ctccccaata aaggcattat gaagcagtct gacgcattct ttcacctgac ggcttggcat
    11881 catcaattcg atactgcgct ccgtgtggtt gactctgatc agttccatgc ctgcgcagat
    11941 ttccaatatc cgggccagaa atcccggatc gaggttgatg cctactccca cgggaatcac
    12001 aaagccgatg cccgtctctt tggacagagg actgatcccg tgtgatccca ggagataatc
    12061 gatctcgctt tcgtattttg cttccacgat caactcaagg cgatcggctt ccagatagta
    12121 tctgaagatc tcaaaactcc agcttttcag caggcttgtg aggacatcgc tgcccggcag
    12181 caggtagcgc aataaatcct ctttgtgggc gatggcgttt ataactctta attccatatt
    12241 ggtttccgtg ttctttttca gcatagtccc aggctggaag ctaaatgatg acttcacttc
    12301 taccgggatg ccgtatttga gggcaaattc caccgctctg ggatggatga ctttagagcc
    12361 tccgtatgcc agagtgagca tgatgtcggg gtcgatcgag gccagcaagc gcggcctttc
    12421 gaccactctg ggatcggcgc tgtaaacccc ctccacgtct gtgaatatct cgcatttatc
    12481 ggccttgaga tagccggcca aggcaaccgc ggatgtgtct gagccgcctc tgccaagcgt
    12541 cgtgatctcc ttttggaggc tgactccctg gaatccggcc acgatcacga tcttgccttt
    12601 ctcgagctcg ctgaggatgc gaaaggcatt cacgttgagg atgcgcgcgt tgccatgatg
    12661 gtcatcggta atgatgccac tttgggaccc agtgaaagaa atcgcttcca acccgcattc
    12721 ctgcaaggca aaggagagta tggacatgct gatcctttca cccgcggtga gcagcatatc
    12781 catttccctg cgattaggcc ttgccgatac agcgtgtgcc atagcccaca gctcgtctgt
    12841 ggtcttcgcc atggcggaca cgaccagcac caacgagtct tgctcgcgct tctcagcggc
    12901 aatcttttcc gcgatgttct tgatcagacc gatactcccc acggaagtac cgccaaattt
    12961 ctttacaatc attgccatag gctttcctaa aattcttaaa ggggctgagt gtcaagagtt
    13021 ttgtctggaa ttagtcccaa acggagtgag attcaatcga gccctgaaaa aaagggagcc
    13081 gaagctccct taacagacag tgtttaatcg gtaagctaat acttacgcgc caccctgaaa
    13141 cccatgaagg cattggttgt tggggtgatg ctggatcgat agatcacagt gcacagagaa
    13201 ggcgtatcag agtatcctcc ccctctgtag caacggctgg agccactcgt tgctccggtg
    13261 taattagtat agctgccggt tgggtatgca gcgtagatat cccagcacca ttcacgaacg
    13321 ttgccactca tcaggtgggt atccacttca ttgaccggtt tactggctac atcccaggtt
    13381 gagtttaaag agttattgtt gtaatatgcg acataagcta cgtcgttgga accactgtag
    13441 gtataattgt gagaggaatt acctccccgc gccgcaaagt gccactccgc ttcagtaggt
    13501 aatctgaagc cgctggcgtt ccagttgcaa ctcaaataaa tatggtttcc cgagttggta
    13561 tcccagcctg agggccaaag gtccggatct tccacattgt tgtatttgta acaggggctc
    13621 aggccaaaat gagagctcaa ccggttacaa aattcgatgg ctttaaacca gctaacattg
    13681 taaacgggat gaaaaacccc aattcctgct cctgaagaag gatttgagcc cataatgttc
    13741 tgatagagtg cctgagtgat ctcagtcttg ccaatataga atgagctgag agtcatattg
    13801 ccggtaccgt tgttaaaagt tccaccagcc acatactcgt caaacatata ggtagccctg
    13861 atcaccgcgc tgggttccca ggagccgtaa tgcgcctggg ctttaatgat ggtgggcttg
    13921 gagatattga tctggccggt gtacagcgta gatgacggcg tgggattact gccgtcggta
    13981 gtgtaacgta ttgccgcatt agtggtggca gaaccgattg ccagatttac ggagacctgg
    14041 taggttccct gggcgatgtt gaagttggga ttagctgccc gcattgtata actcgcggta
    14101 gcaacctcgc ttggtgtgag acctgtcttc caggctttgg ctttgagggt gcagatgttg
    14161 ctgatcgtga gagctccggt atagagcgtg gaggaagtgc tgggctcggt gccgtcgagg
    14221 gtgaagcgga tcgtggagtt ggccgtagag cagctgatcg tggtagtgaa tgtattgttg
    14281 tatacacccg tcaatttctg aatcacaggg gtggcaacag tggtgctgat cgtatatgtc
    14341 ttgctgatga catcactgtt gctaaaacca gattttaccg ctatcgcttt gattgtggtg
    14401 ctttggctga agttgatggg actgccgtaa accggcgacg atgatgacgg aatgctgcca
    14461 ttggtggtgt agtgaattgt ggccccagca gtagcgcagg taagcgtgca ggtttgggca
    14521 gtttcataag taccctcggc gagtgagaag ctgggattga gcggtttcaa gatataactg
    14581 tgagtcacca cagtgctggg tgtgcggccc gcgcagacgg cgatcgcctt gatcgtagca
    14641 ttggtctggg tgacattgta gggaggggtg tacagcggcg agctggcatt gggagtgggg
    14701 ccgttgacgg tatagcggat ctgagcgccc ggagtggtgg aatggatcca gagtccgaag
    14761 ctattgttgt aggtgccact cagatgcgag aatgaaggcg cggctgtatt cagcaccatg
    14821 tttaaactga tctgcgcgtg agatccgttg taactctccc ggaagggata gacccgatac
    14881 tcatagctcc cctgttgtgt aatggagtgg tcccagcaga aggtgctgtt tttgcccacg
    14941 cttttgtatc ccgtttgcca ggagccgcca ttgacgcggc gatcgatctt gtagccggct
    15001 tcgatgttgc aacggtccgt ccagttgagg cgaatactct cacccgacaa ctgatattga
    15061 aggccataag gggcaagcag attggattga atatgctcag gatctacggg attggtgaat
    15121 tccttgttca tgcatcccgc caggagcagc aggacactga tcgcgtacag gagtattctc
    15181 ttcattctca ctctcccagt ttatactgtc ggtaagctct gtcgatgctc ttgtaggtcg
    15241 agcttgtctt ggtggcagag aatatgtagt ttgccagaaa aaacacatcg gctcccaacg
    15301 tgacatagta gtattttttc agttgattgt agcggtcccg aaaactctgt gcttcactgg
    15361 gactcatagt attcatgtag tcgttgtaat tgttttcggc aaagtaatat gtgactgccg
    15421 cggcggctgt ggttcccaga atggccaaca ccgagctgtt gcgatacagc ttccaggcac
    15481 ccagttttct cagcatggct ttgtcgattg ggtccagttt gagctcaggc atttcagaca
    15541 ggcgattggg cttgacttcc actttaaaac tgcggggaat gtagccccat cggtcagctt
    15601 gcagcacata tccacccgag tagagagtca gggaattctt gtatttgatc tctgatttgc
    15661 tgcggtcatc caccagggtg acgctgattt caggatcgat cttcttgatt ttaaactgac
    15721 cggaagatat cttgtcgagc ttgacgaaga cgttgttgtc tatactgtta gagagcttgg
    15781 cgcttttcga gaccgggaag tagtccttga gctcaacgct gaacaagtat tctccaggag
    15841 gatacctgcc aatcagtgga tccaccgtca gtggtgtaac ccccaatttc atcccgttga
    15901 gcgagacgac agccccgctg ggttcggaag tcaccgtaat cccgcagtag tttgaaaaca
    15961 gcttggtttt gatttttttt ccggagacat ctggcctgat atccattttt tgggtgacgt
    16021 ggtccggttt gctgatcgtg attgaataag tcttatctgt gtcgctggca aagatatagt
    16081 taccgaaagt gatgttatcc ggaaatcctg atatgttgat ggaagctgaa ctgtcgcagg
    16141 aaagctcggt ccacagcttg tagagcgagg aacctacatc ccggaagctg aagtggatat
    16201 ctatcgtttc gccgatggaa acacccacct ggcggctcaa aattcccttt gaacccacaa
    16261 aatccacctg atggacccca ggcgccatgt cgcgctttga aatctgcagg atggccgtga
    16321 tcgtctccac aaacacgcca tccacataca ccgatccgga gtcgtctgcg gtcatgcgga
    16381 aggctcccgc ccgcgtttca agttccagag aggggggcgg cacataagct ggaggtctgg
    16441 tttgttgtgg agtaatcggt accgcatcct gtttgagtgc gaacacaaaa tcgcctttgt
    16501 ccaaagcaat gttggcaatc ttgccata
//
LOCUS       DAOSFN010000046        16228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_2935_length_16228_cov_8.413281, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000046 DAOSFN010000000
VERSION     DAOSFN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 16228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16228
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2935_length_16228_cov_8.413281"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1178
                     /locus_tag="PLO35_04490"
     CDS             126..1178
                     /locus_tag="PLO35_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HOH78957.1"
                     /translation="MKKNQLRLNQTLAALVDEYIGSVEPVSSRILNEKYLTQVSSATL
                     RLDLMKLEQQSLICQPHTSAGRIPTIRGYRQYLRMIEPEHQRVRFERMNILRELLIGN
                     YKDTPRALHFIMQMLARETDQLSFVAEPEVSNGYLAKLDVFPIGSRKFIFVMSLDSGL
                     DKTVVMKCEYDITDTQLRKLVRYLNDELVGLRIYDIANRVLVEMRENMHQDNALVSIF
                     LRELHKAFIELTDFFINFDGSISFLEQPEFDSKMSILSFMNLIQRQDQLLKLMREHDS
                     GGRVKVLMGEDFNEPQWQEFCLIFGRYEVFGIPGFLGVVAPLRTDYRKLIPLIRDVIF
                     TITDTTKRGMIVPKEK"
     gene            1180..1785
                     /gene="grpE"
                     /locus_tag="PLO35_04495"
     CDS             1180..1785
                     /gene="grpE"
                     /locus_tag="PLO35_04495"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOH78958.1"
                     /translation="MSRKKRHGDDHKPEAGVTPERQIETEQEEMEIKIEDSGPVREAK
                     LEQEVAEWKDKYLRNMAEFENFRKRSNQEKADWIKLATQKLALDICDVLDNFERALAQ
                     TGEEHKEDKLIKGFAMIEQQLRTVLERENIRKIDALGKDFDPQWHEALAHIPSDYDEN
                     IVAAVIQNGYMIHDKVLRPARVAVSSGPLKAEPEEQDIEQE"
     gene            1807..3768
                     /gene="dnaK"
                     /locus_tag="PLO35_04500"
     CDS             1807..3768
                     /gene="dnaK"
                     /locus_tag="PLO35_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOH78959.1"
                     /translation="MGKIIGIDLGTTNSCVAVVEGGKPVVITNAEGGRTTPSVVAFTK
                     DGQRLVGQLAKRQAVTNPTNTVFSIKRFMGRAHGEVGNEIKQVPFKVTSGIKGQAAVH
                     IDTENKDFTPQEISAMVLTRMKETAEAYLGQTVTEAVITVPAYFNDDQRQATKDAGKI
                     AGLDVKRIINEPTAAALAYGMENKKEQKVAVYDLGGGTFDISILDISQGVVEVLSTNG
                     DTHLGGDDFDKRVIDWILENFKKQEGIDLSRDPMAMQRLREAAEKAKIELSGTQTTNI
                     NLPFITADATGPKHLSLDLSLAEFNRLTEDLVQRTLGPCRQALTDAKLSASEIQEVLM
                     VGGSTRIPAVGEAVEKFFGKKPNRSLNPDEVVAIGASIQGAILSGDDNVSEVLLLDVT
                     PLSLGIETLGGVCNTLIPRNTTIPTKKSQIYSTAADNQTAVTINVLQGERPMAQDNKS
                     LGRFDLVGIPAAPRGVPQIEVTFDIDANGILHVSAKDKGTGKEQSIVIDRAGSINKDD
                     IDRMIKDAEMHAEEDKKRQEFITAKNELDGLIFSTERSLQEYGDKLSESEKSELEAEI
                     KTAKDRMEKATDAAEMESIKSDLAKKAQKLGEIIYKQAQQQQGGAGFDPNAAGFNPED
                     FQQGPQAQDAGPKKEDGPIDADFEVVDDK"
     gene            3973..5130
                     /gene="dnaJ"
                     /locus_tag="PLO35_04505"
     CDS             3973..5130
                     /gene="dnaJ"
                     /locus_tag="PLO35_04505"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOH78960.1"
                     /translation="MAKRDYYEVLGVNKSADEAEIKKAYRKLAMQFHPDKNPDNKEAE
                     EKFKEASEAYEILSDKDKRQVYDQYGHAGIDRQFGGGGFSWDNFTHFDDISDLFGGGG
                     FSSIFETLFGGGGFGGRGSQRSSNRGEDLQIELSLTLKEIALGAEKTIKIGVKEACDR
                     CGGSGSEDGKVESCSQCHGTGQVRTVRQSLFGQMQTVSECPSCRGEGKIVKNKCSKCY
                     GEGRQSKVKEIQVKIPAGVEESQYIRLRGQGNIGPRSGSHGDILVLIHEKQDDVFERD
                     GNNIILEYPVTVSQAVLGDEVTVPTLTGQVKMKIPAGTQSGRLFRLKGQGIQGLHSYS
                     RGDQIVRAVVVTPTKLSRDETELYQKLKEFDRKREMKPGHSFLHKLRDIFN"
     gene            5140..5871
                     /locus_tag="PLO35_04510"
     CDS             5140..5871
                     /locus_tag="PLO35_04510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOH78961.1"
                     /translation="MPSVYFPNLTSSDRILVLSGEEFHHLARVARVRVGDVVMLNSGK
                     GMLARARVMEIGKREAKLEIDELMANMQFPAPYAIAFSLLKNRHDELLVEKCTELGAG
                     QFFPMVCDFSIRKSANDSRLERIALAAIKQCDNPILPVLEQPTELKAVLVKAREYGYT
                     PILCSELKPDLWLDDLKLPAETKPCFFIGPEGGWSESEIDLLQQENVTSISIGHLITR
                     AETAAITIAAQWVAYANRLRTSGTK"
     gene            complement(5835..7079)
                     /locus_tag="PLO35_04515"
     CDS             complement(5835..7079)
                     /locus_tag="PLO35_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HOH78962.1"
                     /translation="MVHADKIQLLCFFLVSYLLSRVFVRFELPKRLVWWLIEEKHLSI
                     SRLSWLIIAGTALLSMLIANVVTLMAMIPVLDLIQKEFKGSPQEHKRFSTLMMLSAVW
                     GANIGGMGMLTGTTTNGILIAMYEAYKFQISNQFTFLSWMSWGIPLAILLSVIGWLVL
                     MLVFRPARQLAGGELRAQLCCGDSNPQAQRLAVHLALLFLVSAAVLSSSMSFFPEHRL
                     TLMVISISWTLGFLYLLFVHKFALGVEEVRQILLPRSHIFHDLPRKGLLWILGGVVIT
                     AVLVALGFPDSVARASIQWISQDQSVILLLLIVGAITTFSTELVSNTVIQIAMFMSLF
                     PLSRVYPEISWQVMLIISLSSTCAFMSPIATPSNGLGFGSTRRVALPYMLMAGLLMNI
                     ASLLTITLWVHYFVPLVLSLFA"
     gene            complement(7084..8139)
                     /gene="lpxK"
                     /locus_tag="PLO35_04520"
     CDS             complement(7084..8139)
                     /gene="lpxK"
                     /locus_tag="PLO35_04520"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOH78963.1"
                     /translation="MLSNAFLERHLFQRSRLSDLLYPVSLVYSAIQKARGKKGLAQAI
                     RLPYPLIGIGNIVSGGSGKTPFTLALAKAVCADAYRPAVSHRGYKGKLEGTPSLISRG
                     EGPLFPAADCGDEAWLMAKTLSGIPVVVGKDRLRAVDLLSGLEPRPDIVLMDDVFQNH
                     KLIKDIDIVCFDAAVGIGNGRLIPAGYLREGLPALKRAGLVVITRKQVSIDPAPLIEA
                     IRPFCPWVEVLDYSLTGIEDSEGGAIETGSVQKSILVSGIAFPTSFERLAATAGITWT
                     RHFAFRDHYHFQSREALKPLLDYIRAERIEHVCCTAKDVAKLSQHKDLKPLLAVLVLG
                     PDQNRVDELWRRIKERL"
     gene            complement(8144..8524)
                     /locus_tag="PLO35_04525"
     CDS             complement(8144..8524)
                     /locus_tag="PLO35_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH78964.1"
                     /translation="MPKLICIVDDEDELIQNLKAEFGALRPEWDVRVFSNGMDALRLI
                     LSGGVDLVLTDIAMPDMDGYELYWRVKEHDPKIPVIMMTGFGYDPNHVLVRAKSDGLQ
                     DVLYKPFDVEDLAMKIDLRLAESQ"
     gene            complement(8524..9405)
                     /locus_tag="PLO35_04530"
     CDS             complement(8524..9405)
                     /locus_tag="PLO35_04530"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOH78965.1"
                     /translation="MYDKLRQLSLVKQSRQGKVRDIYDLGDTLLLVTSDRVSAFDVVF
                     PDLIPDKGIILNKIAVSVFNATRHIISNHLISDRVEDFPLEFKPFEDYLRGRSMLVRK
                     TRVIPFECIVRGYISGSAWSEYTRTGTVGGMLIAEEMKESQKFGQPLFTPSTKAEEGH
                     DVNISYREMLGHMDQWIAELIKNKSVELYNYGHELMLKHHIILADTKFEFGISGGEIV
                     LIDEALTPDSSRFWAVDDYEIGKAPKSFDKQFIRDHLNQSGWNKLPPAPQLPEEVIRQ
                     TRTKYLQIYQIMTGETL"
     gene            complement(9410..10402)
                     /locus_tag="PLO35_04535"
     CDS             complement(9410..10402)
                     /locus_tag="PLO35_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78966.1"
                     /translation="MERVKIAITDSDNTAKLPSGWCLMAFMEGDEVLHLGISPNAGKR
                     YAYLKERAKEDAGVDELLKTASSLQVEKVNSALDALIALKTGVHDARPRLQNSYRPWQ
                     NYVYLAIDAYHFPFIRITPDTNDDWIYLGPFRDRFFLADVLDCVGRILRLPRCESDVW
                     PCDKLDRGLCGGYCRGLDSSFREPDTDAGLSNDPKASGESDYSLEKLDSLLKESFLSP
                     SQGILEMLKHERDKYFDDLEFIKADLLDEEIALQQKYRDWLIFLYAARQLNWEDDRLL
                     VERGRIRRCTYRGRDYLFAPDSTPYRENESLALNLDSVDEARILYEYYKDYSQG"
     gene            complement(10635..11369)
                     /locus_tag="PLO35_04540"
     CDS             complement(10635..11369)
                     /locus_tag="PLO35_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOH78967.1"
                     /translation="MHKTMFILSILVLVGGLLWAQGYELRVLTENYPPLTYMEEGAIK
                     GYGADVVSAIQKLLGTEFPIELHMWEKAYELALNEPNILLFTMEKTAEREKLFQFVGP
                     LGANTAWFYVPAGSKIEIKDLDAARKLNAIATTTNWFTEQELIKQGFENLKSVADPIE
                     TIHMVLKGKVDASVYTDLTLPQLAKEAGVAPNELKPALELMRSEYFLAFSAKTDPEIV
                     KAWSEAFNKLQESGELKSIKANWDIP"
     gene            11514..12338
                     /locus_tag="PLO35_04545"
     CDS             11514..12338
                     /locus_tag="PLO35_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78968.1"
                     /translation="MKLARIVVIAVLLLCGLAAMGLKGDDRPALKVSGLAYPYKLDSV
                     DRFEDNGFDFGEGDLVDLGNHKTFSWDEVLRMVDSQKGAKTEYSLNWDEIQNLVYNMI
                     FVLSAGQIYRYHLGERLLHAMIHPQGSSKVPLGTHHIRLMLLDPTQEDVRREMRDRLI
                     ALLKDENTEALWYLQKTSYLSTNAKYIQEADPIFKASYRASIQNRLKELISAVDQTDY
                     QLKAVQSYPGQWELLEQKGNKLYSYTPRTLNLICYRLGKDAAQKLKSYAQQILDAF"
     gene            12706..15426
                     /gene="ppdK"
                     /locus_tag="PLO35_04550"
     CDS             12706..15426
                     /gene="ppdK"
                     /locus_tag="PLO35_04550"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOH78969.1"
                     /translation="MTDTKRVYLFGNGQAEGKADMKNLLGGKGANLAEMNLIGLPVPP
                     GFTITTDTCNEYTKLGRDAVSALLKSEVEAAIRHLEKLMNMEFGSDANPLLLSVRSGA
                     RASMPGMMDTILNLGMNDKAVQGIIKKTGNERFAWDSYRRFVQMYGDVVLGLKPASKE
                     EEDPFEVIIHKMKHDKGVTNDLDLSADDLKQLVALFKAAVKENTGHDFPESPWEQLWG
                     AIYAVFDSWNNDRAIFYRQLNNIPGEWGTAVNVQAMVFGNMGNSSATGVAFTRDAATG
                     ENLFNGEYLINAQGEDVVAGIRTPQQITKEGSQRWAKLAGVSEADRVAKFPSLEEAMP
                     TVYQELFAAQRKLENHARDMQDIEFTIQEGKLWMLQTRNGKRTGFAMVRIAMEMLRDG
                     MIDDQTALLRMEPNKLDELLHPVFSRQGLAAAKVITKGLPASPGAATGQIVFFADDAE
                     AWAAQGKKVVLCRVETSPEDLRGMNVAQGILTARGGMTSHAAVVARGMGKCCVSGAGA
                     LVIDYKLRTMSVDGNVYKEGDWISLNGSTGQVLEGKIETVEPELSGDFGALMELADKH
                     TRMKVRTNADTPKDARLARGFGAQGIGLCRTEHMFFEGDRIKAMREMILAEDETGRRK
                     GLHKLLPMQRSDFEGIFEAMDGFPVTVRLLDPPLHEFVPHEEEQQQELADELGISLRK
                     VKESVAALHEFNPMLGHRGCRLGNSYPEITEMQARAIIEAAINVTAKGIKVLPEIMVP
                     LIGTPAEFELQEEIIRATAEKVFAEKGTRVDYLVGTMIEIPRAALVADKIAVRAEFFS
                     FGTNDLTQMTFGYSRDDSGVFMPVYLAKNLLKHDPFQILDQEGVGQLVEMGTLRGRMT
                     RPELKVGICGEHGGEPSSVEFCHRVGMNYVSCSPFRVPIARLAAAMAAIR"
     gene            complement(15500..15817)
                     /locus_tag="PLO35_04555"
     CDS             complement(15500..15817)
                     /locus_tag="PLO35_04555"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOH78970.1"
                     /translation="MAGIPDRTELIEIINRYVELVSQRIRVESVYLFGSYVDGKPDDY
                     SDIDLLIVSPDFGEDLFEEQVRLMIARRQVDLRIEPHPVPSGELDSNILFAMAGDQML
                     RVV"
     gene            complement(15834..16181)
                     /locus_tag="PLO35_04560"
     CDS             complement(15834..16181)
                     /locus_tag="PLO35_04560"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOH78971.1"
                     /translation="MRKEELIKYWLDSAEMDFQSMKRLFDTGEFAWSLFLGQLVLEKA
                     MKAVYSQKVGNNAPRLHDLNRLALLAGLDVSAEDQAHLDTISLFNINARYPDVKLAST
                     KSAPRIMQKSILI"
BASE COUNT         4067 a         4384 c         4338 g         3439 t
ORIGIN      
        1 cgttttgatt ttttagcagt ctcactttta gattgacaat tttcctgtgt cgattatctt
       61 gtctcttgaa ttagcagttc cgctgccgga gcgctaagat tcaatgttgc ccaaggtgct
      121 accccatgaa aaaaaatcag ttacgtctga atcagaccct ggccgcgctg gtggatgaat
      181 acatcggcag cgtagagccc gtatcctcca ggatactgaa cgagaagtat ctcactcagg
      241 tgagttcggc cactttgcgc ctcgatctga tgaagctgga acagcagagc ctgatctgcc
      301 agcctcacac ttcggccggc cggattccca ctatcagagg ttatcgtcag tatctgcgga
      361 tgatcgagcc cgagcaccag cgggtgagat tcgagcgcat gaacatcctg cgggaactgc
      421 tgatcggcaa ttataaagac acgccccggg ctttgcattt tatcatgcag atgctggcca
      481 gggagactga tcagctcagc tttgtggccg aacccgaggt ttccaacggt tatctggcca
      541 agctggacgt cttccccatc ggatcgcgca aattcatctt cgtgatgagc ttggacagcg
      601 gactcgataa aacagtggtg atgaaatgcg agtatgacat caccgacacc caactgcgca
      661 agctggtgag atatctgaac gacgagctgg tgggcttgcg catctatgat atcgctaacc
      721 gggtattggt ggagatgcgg gaaaacatgc atcaagacaa cgccctggtc agtatatttc
      781 tgcgcgagct gcacaaggcc ttcatagagc tcaccgattt ctttatcaac ttcgacggca
      841 gtatcagttt tctggagcag ccggagtttg attccaagat gagcatactg agctttatga
      901 acctcatcca gcgccaggac cagctgctga agctgatgcg cgagcacgat tccgggggca
      961 gggtgaaagt gctgatggga gaagacttca acgagccgca atggcaggaa ttctgcctca
     1021 tctttggacg ctacgaagtg tttggaatcc cgggatttct gggagttgtg gcacctctgc
     1081 gcaccgatta cagaaagctg atacctctga tccgggacgt catcttcacg atcacagaca
     1141 ccaccaagcg cggcatgata gttcccaagg agaaatgaaa tgagcaggaa gaaaagacac
     1201 ggcgacgacc ataaaccgga agcgggagtg actcctgaac ggcagataga aacagagcag
     1261 gaagaaatgg aaataaagat cgaagacagt ggtccggtcc gcgaggccaa attggaacag
     1321 gaagtggccg aatggaagga caagtatctg cgcaacatgg cggagtttga aaacttccgc
     1381 aagcgcagca atcaggaaaa ggcggactgg attaagctgg ccacccagaa gctggccctc
     1441 gatatctgtg acgtactgga caattttgag cgcgctctgg cccagacggg tgaagagcac
     1501 aaggaagaca aactgatcaa aggctttgct atgatcgagc agcagctccg caccgtcctg
     1561 gagcgtgaaa acatccgcaa gatcgatgct ttgggaaaag atttcgaccc tcaatggcac
     1621 gaagccctgg cccatatccc cagcgattat gatgaaaaca tcgtggcggc cgtgatccag
     1681 aacggctaca tgatccatga taaagttttg aggcctgccc gcgtggcagt ctcatccggt
     1741 cccctgaaag cagaaccgga agagcaagac attgaacaag aataatatga gtaccatgga
     1801 ggaataatgg gaaagataat tggaattgac ctcggtacca ccaactcatg cgtcgcggtg
     1861 gtcgagggtg gcaaacccgt agtaataacc aatgccgaag gcggacgcac cacacccagc
     1921 gtggtggcct tcaccaaaga tggacagcgt ctggtaggcc aactggccaa gcgtcaggct
     1981 gtcaccaacc cgacgaacac agtgttctcg atcaagcgtt tcatgggccg cgcccatggc
     2041 gaagtgggca acgagatcaa gcaagtcccc tttaaagtga cctctggcat caaaggccag
     2101 gcggccgtgc atatcgacac cgaaaataaa gatttcaccc cacaggaaat ctcggctatg
     2161 gtgctcacca gaatgaagga aaccgccgaa gcctatctgg gccagacggt taccgaggcc
     2221 gtgatcactg tgcctgccta ttttaatgac gaccagcgtc aggccaccaa agatgccgga
     2281 aagatcgccg gactggacgt gaagcgcatc atcaacgagc ccacagccgc ggccctggct
     2341 tatgggatgg aaaacaagaa agagcagaaa gtggccgttt atgacctggg cggcggcact
     2401 ttcgacatct cgattctgga tatctctcag ggcgtggtgg aagtgctgag caccaacggc
     2461 gatacccacc taggtggaga tgactttgac aaacgtgtca ttgactggat actggaaaac
     2521 ttcaagaagc aggaaggcat cgacctatcc agggacccca tggctatgca gcgcttgcgt
     2581 gaagcggctg aaaaggccaa gatcgagctt tccggcactc agaccaccaa catcaatctg
     2641 cccttcatca ctgcagacgc caccggtccc aaacatttga gcctggatct cagtctggcg
     2701 gaattcaacc gcctcacgga agacctcgta cagcgcacct taggcccctg ccgtcaagct
     2761 ctgaccgatg ccaaactgag cgcttccgag attcaggaag tcctgatggt aggtggcagc
     2821 acccgcatcc cggcagtggg agaagcggta gaaaagttct ttggtaaaaa gcccaaccgc
     2881 agcctcaatc cggatgaagt ggtagcgatc ggagcgtcca tccagggagc catcctgtcc
     2941 ggagacgaca acgtgagcga agtgctgctc ctggacgtca ctcctctttc tttgggaatc
     3001 gagaccctgg gcggggtctg caatactctc atcccccgca acaccaccat tccaaccaag
     3061 aagagccaga tatattcaac cgccgcggac aatcagaccg ccgtcaccat caacgtcctg
     3121 cagggtgagc gtcccatggc ccaggataac aaatcattgg gccgctttga cctggttggc
     3181 atcccagccg ctcccagagg ggttcctcag atcgaagtca ctttcgatat cgacgccaac
     3241 ggtatcctgc acgtctcagc caaggacaaa ggcaccggca aggagcaatc gatcgtgatc
     3301 gaccgggccg ggagtatcaa caaagacgac atcgaccgca tgatcaagga cgctgagatg
     3361 cacgccgagg aagacaaaaa gcgccaggaa tttatcactg ccaaaaacga actggacggc
     3421 ctgatcttca gcaccgaacg cagcctgcag gaatatggcg acaaactcag cgaaagcgaa
     3481 aagagcgaac tggaagccga aatcaagacc gccaaggacc gcatggagaa agccaccgac
     3541 gcggctgaga tggaaagcat caagagcgac ctggccaaga aggcgcagaa actgggtgag
     3601 atcatctaca agcaagccca gcaacaacaa ggcggcgcag gatttgatcc caacgctgct
     3661 ggtttcaatc ctgaagattt ccaacagggc ccccaggctc aggatgccgg ccccaagaaa
     3721 gaagacggtc ccatcgacgc cgactttgaa gtggtggacg acaaataatc cgcatacagg
     3781 ggcgggtact tcccgcccca ctattaacct ggatattgat caccagttcg gccaatgaca
     3841 aactcacatc cgcaaacgag ggcgtttgcg gccctgtctg gaatgtcgga ttccggtctg
     3901 agaaacgtgt atcccttcaa tcgcaaacga ggacgtttgc ggttccactg taaaacatgc
     3961 ctgggagagt aaatggcaaa acgtgattat tatgaagtgc tgggcgtgaa caaaagcgcc
     4021 gacgaagcgg agatcaagaa ggcctatcgc aagctggcca tgcagttcca tccggataag
     4081 aatccagata acaaggaagc ggaagagaag tttaaggaag ccagcgaagc ctacgagatt
     4141 ctatctgaca aggataaacg ccaagtctat gaccagtatg gccatgccgg catcgatcgt
     4201 cagtttggcg ggggcggttt ctcctgggat aatttcaccc acttcgacga tattagtgac
     4261 ctctttggcg gaggcggttt cagcagcatc tttgagactc tctttggtgg cggcggtttc
     4321 ggaggacgcg gttcccagcg ttccagcaac cggggggaag accttcagat cgagctctcc
     4381 ctcacgctca aagaaatcgc gttgggcgcc gagaagacca tcaagatcgg tgtcaaggaa
     4441 gcctgcgacc gttgtggagg atccggcagc gaggacggca aggtggaatc ctgctcccaa
     4501 tgtcacggca ccggtcaggt gcgcacggta cgtcagagcc tgtttggcca gatgcagaca
     4561 gtctccgagt gcccctcctg ccgtggtgaa ggcaagatcg taaaaaacaa atgcagcaag
     4621 tgctacggcg aagggcgtca gagcaaggta aaggaaatcc aggtcaagat tcctgccgga
     4681 gtggaggaaa gccaatacat ccgcctgcgc ggacagggaa acatcgggcc ccggagcggc
     4741 agtcatggag atattctggt gctcatccat gaaaagcagg atgatgtctt tgaacgggat
     4801 ggtaacaaca tcatcctgga gtacccggtc actgtttctc aggccgtgct gggtgacgaa
     4861 gtcaccgtgc ccacgctcac ggggcaggtt aagatgaaaa tacctgccgg cactcagagc
     4921 ggcaggctgt tcaggctgaa gggacaaggc atccagggtt tgcactccta ttccagagga
     4981 gaccagatcg ttcgtgccgt ggtagttacg cctaccaagc tatcaaggga tgaaaccgag
     5041 ctatatcaga agctgaaaga gtttgaccgc aaacgggaaa tgaagcccgg acacagcttc
     5101 ctgcataagc tgcgggatat ctttaattga ggtaagccct tgccgtcggt atacttcccc
     5161 aacctaacat cctcagacag gatcctcgtt ctcagtggtg aagagttcca ccatctggca
     5221 agagtggcaa gagtgcgggt gggagacgtc gtcatgctca acagcggcaa aggtatgctt
     5281 gccagagcga gagtaatgga gatcgggaaa cgggaagcga agctggaaat tgatgaattg
     5341 atggcaaaca tgcaattccc cgctccctac gcgatagcgt tctcgctgct caaaaaccgc
     5401 cacgacgaac tgctggtgga gaagtgcacc gagttgggag ctggtcaatt cttccccatg
     5461 gtctgtgact tcagcatccg caagagtgca aacgacagtc gtttggagcg gatcgctctg
     5521 gcagccatca aacagtgtga caatcccatc ctgcctgtcc tggagcagcc aacagagctg
     5581 aaagcagttc ttgttaaagc cagagaatac ggctacacac ccatcctctg ttccgagctt
     5641 aaaccagatc tgtggctgga tgacctgaaa ttgcctgcgg agacgaagcc ttgcttcttt
     5701 atcggtccgg agggtggctg gtcagagagt gagatcgacc ttctacaaca ggaaaacgtg
     5761 acttcgatat cgataggcca cctgatcacc agagctgaaa ccgcggcgat caccatcgcg
     5821 gctcaatggg tggcctacgc aaacaggctc aggacgagcg gcacgaaata atgcacccag
     5881 agggtaatcg tcagcaggct ggcgatgttc atcaataatc ccgccatcaa catgtaaggt
     5941 aaggcgaccc ggcgggtgct gccaaaaccc agaccattgg agggagtggc gatcgggctc
     6001 ataaaagcgc aggtgctgga aagtgagatg atcagcatca cctgccatga aatctcggga
     6061 tatactcttg agagcggaaa caagctcata aacatggcga tctggatcac cgtattgctc
     6121 acgagttcag tggaaaaggt ggtgattgcc cccacgatca ggagcagcaa aatcaccgac
     6181 tgatcttggc tgatccattg gatggacgct ctggccacgc tatctggaaa acccagcgca
     6241 accaggacgg cggtgatcac aactcccccc aatatccaca acaacccttt gcggggcagg
     6301 tcgtgaaata tatgcgagcg aggcaggagt atctgccgca cctcttcaac ccccagggca
     6361 aacttatgca cgaacaagag atacaggaag ccaagagtcc agctgatact gatcaccatc
     6421 agcgtgagcc ggtgctcagg gaaaaaactc atgctcgatg acagaaccgc tgcagagacc
     6481 agaaacagca gggccagatg gacggccagc ctttgggcct ggggattaga gtctccacag
     6541 caaagctgag cacgcagttc tccaccggca agctggcggg cgggacgaaa caccagcatc
     6601 aacaccagcc atccaatcac gctcagcaag atcgccagcg gaatacccca gctcatccag
     6661 gaaagaaagg tgaattgatt gctgatctgg aacttatagg cttcatacat tgcgatcaga
     6721 atcccattgg tagtggtgcc ggtcagcatg cccattcctc cgatattggc accccatacg
     6781 gcagacaaca tcatcaatgt ggaaaagcgc ttatgctcct gcggggatcc cttaaactct
     6841 ttctggatga ggtccaaaac cggtatcatc gccatcaggg tgactacgtt ggcaatgagc
     6901 atggacagca gggctgtccc tgctataatc aaccagctga gtctggatat gctgagatgc
     6961 ttttcctcta tcagccacca caccaggcgt ttggggagct cgaaacggac aaacacacgt
     7021 gacagcagat aggataccag aaagaagcac agcagttgga tcttatccgc gtggaccaag
     7081 gtttcagagc ctctctttga tccttcgcca gagctcgtcc accctgttct gatcgggtcc
     7141 cagcaccagt actgccagca gcggcttcaa atctttgtgt tgagataact tggccacatc
     7201 tttagccgtg cagcagacat gctcgatcct ctcggcgcgg atgtaatcca acagcggttt
     7261 gagcgcttcc cggctttgga aatgataatg gtcacgaaac gcgaaatgcc gcgtccaggt
     7321 gatcccagcc gttgcggcaa gcctttcaaa gctggtgggg aaagctattc ccgataccag
     7381 gatggatttt tggaccgatc ccgtttctat agccccgccc tctgaatcct caatacccgt
     7441 aaggctgtaa tccaggactt ccacccaggg gcagaaaggc ctgatcgcct cgatcagagg
     7501 agcgggatca atggagacct gtttgcgggt tatcaccacc agtccggccc gtttcaaagc
     7561 aggaagcccc tcccttagat aaccagccgg gatcaatctg ccgttgccga ttcccaccgc
     7621 ggcgtcaaaa cagacgatat cgatgtcttt gatcagttta tggttttgaa acacgtcgtc
     7681 catcaacacg atatccggtc gcggttcaag cccggaaagc agatcaacag cccgcaaacg
     7741 atccttgcca accaccacgg gaatgccgga aagcgtcttt gccattagcc aggcttcgtc
     7801 tccacaatcg gccgccggga aaagaggtcc ctcgcccctg gaaatcaggc tcggagtgcc
     7861 ttccagcttg cctttgtagc cacgatggga caccgcggga cgataagcat cagcgcaaac
     7921 cgctttggcc aaagccagcg tgaaaggggt ttttccgcta ccgccactca cgatattacc
     7981 gatcccgatc aggggatagg gtaaccggat ggcttgcgcg aggccttttt tcccacgggc
     8041 tttctggata gcggagtaga cgagcgatac cggatacagc aggtcgctca ggcgcgagcg
     8101 ctgaaataag tgcctctcca gaaaagcgtt gctgagcact ctgctattga gattcagcca
     8161 gacgcaagtc gattttcatg gccagatctt ccacatcgaa gggtttgtaa agcacgtcct
     8221 gcagcccgtc tgattttgcc ctcaccagca cgtggttggg atcgtaacca aagccggtca
     8281 tcatgatcac cggaatcttg ggatcatgct ctttgacgcg ccagtaaagc tcatagccgt
     8341 ccatatcagg catggcgata tcggtaagca cgaggtccac tcctccagag aggatcaatc
     8401 ggagggcatc cattccgttt gagaagaccc gcacatccca ttcgggacgc agggcaccga
     8461 actcggcttt gagattctga atgagttcgt cttcgtcgtc gacaatacag ataagcttgg
     8521 gcatcagagg gtctctccag tcattatctg atatatttgc aggtatttag tcctggtttg
     8581 gcggatcact tcttccggga gttgaggggc cgggggcagc ttgttccagc cgctttgatt
     8641 cagatgatcg cggataaact gcttgtcgaa gcttttgggg gctttgccga tctcatagtc
     8701 gtccacagcc caaaacctgg atgaatctgg cgtgagagct tcatcgatga gcacgatctc
     8761 gcctccgcta atgccaaatt caaacttggt gtcggccaga atgatgtggt gcttcaacat
     8821 cagttcatgg ccatagttat acagctccac ggatttgttt ttgatcaatt cggctatcca
     8881 ctggtccata tggcccagca tttcacggta gctgatattg acgtcgtgac cctcctcggc
     8941 cttggtggaa ggggtgaaaa gcggctgtcc aaatttctgg ctttccttca tctcttcggc
     9001 gatcagcata ccgcccaccg tgccggtcct ggtatattca ctccaggcgg agccgctgat
     9061 atatcctctc actatgcatt caaagggaat cacgcgggtc ttgcggacca gcatgctgcg
     9121 gcccctcaga tagtcctcaa agggcttgaa ctccagcgga aagtcttcca ctctgtcgct
     9181 gatgagatga ttggagatga tatggcgggt ggcgttgaat acactcaccg ctatcttgtt
     9241 gaggattatc cccttatctg ggatcagatc tggaaacacc acgtcgaagg ccgacaccct
     9301 gtcgctggtg accagcagga gcgtatcgcc caggtcatag atgtctctca ccttgccctg
     9361 acgggattgt ttaaccaaac tgagttgtct tagcttgtca tacataaatc tatccttgac
     9421 tatagtcttt gtaatattca tagagaatcc tggcctcgtc cacgctgtcc agattgagcg
     9481 ccaggctttc gttttcccgg tagggagtgg aatccggagc aaagagataa tcccttcccc
     9541 gataagtgca gcggcgaatc ctgccacgtt ccaccaggag tctgtcatcc tcccaattga
     9601 gctgccgggc cgcgtacaga aagatcagcc agtcgcgata cttttgctgc aaagcaattt
     9661 cttcatccag gaggtccgcc ttgataaact ccagatcatc aaaatacttg tcgcgttcat
     9721 gcttcaacat ctcaagaatc ccctggctgg ggctcagaaa gctctccttc agcaggctgt
     9781 caagcttctc cagtgagtaa tcggattcac cggaggcctt gggatcattg cttaacccgg
     9841 catctgtgtc aggctcgcgg aagctggaat ccagccctct gcaatacccc ccgcaaagtc
     9901 ctctgtccag cttgtcgcaa ggccagacat ctgattcaca ccgcggcagc ctcaggatgc
     9961 gtcccacgca gtccagcacg tcagccagaa aaaaacggtc gcggaagggg cccaggtaga
    10021 tccagtcatc attggtgtcc ggcgtaattc tgataaaggg aaagtggtag gcgtcgatag
    10081 ccagatacac atagttttgc cagggacggt agctgttctg taagcggggc cgcgcgtcat
    10141 gaactcctgt tttgagagcg atcagggcat ccagagcgct gttcactttc tccacttgca
    10201 ggctggaagc tgtcttcagc agttcatcaa cgcccgcgtc ctctttggcg cgctctttca
    10261 aataagcata gcgcttcccg gcattggggc tgatccccaa atgcaggacc tcatcccctt
    10321 ccataaatgc catcaggcac catccggagg ggagtttggc tgtattgtcc gagtccgtga
    10381 ttgctatctt gacgcgttcc ataagagcca tcctttttag ctggcgcttt gtgtcaactg
    10441 aatcgttaag gttttagaag tgaggcaagt ttccggcaga ttacgcggat gggaacgcag
    10501 atctcgcagt ttgttgtcag gataagggag tggaatgggt ttagaaggtt gatagggttg
    10561 agagagttga gaaggcaggg ggtatcaacc ctctgaacct tcccaatcct ctcaacccaa
    10621 agagggaagt ttagttaggg aatgtcccaa ttggctttaa tacttttcag ctctcccgat
    10681 tcctgcagct tgttgaaagc ttcactccag gctttcacga tctcaggatc ggtcttggcg
    10741 gaaaatgcca gaaaatactc gctgcgcatc agttccaaag caggcttgag ctcgtttggg
    10801 gccactccgg cttccttggc aagctgaggc agagtgagat cggtgtagac ggaggcatcg
    10861 accttgccct tcagcaccat gtggatggtt tcgatgggat cggccacgct tttgaggttt
    10921 tcaaagccct gcttgatcaa ttcctgttct gtaaaccagt tggtggtggt ggcgatggcg
    10981 ttcagtttgc gggcggcatc aaggtctttg atctcgattt tgctgccagc aggcacgtag
    11041 aaccacgcgg tattggcacc caatggtcca acgaactgaa agagcttttc gcgttcggcg
    11101 gtcttttcca tggtaaagag cagaatattg ggctcattca gggccaattc ataggctttt
    11161 tcccacatat gcaactcaat ggggaattcg gtgcccagca gtttctggat ggcggatacc
    11221 acgtccgcgc cgtatccctt gatcgcacct tcctccatat acgtgagggg aggatagttt
    11281 tcagtgagga cgcgcaattc ataaccctgg gcccagagca ggcccccaac cagaacaagg
    11341 atactcagga taaacatagt tttgtgcatg aggttctcct gtagatatat ttttacaagt
    11401 gtgagcgttc cccgattccc gtcaagtttt ttatccgcgg atgttttttc ttgacagacg
    11461 ctgcccacca tcccactggg tttcagggat cggatgaccg gtcgggagga atcatgaagc
    11521 tcgccagaat cgtcgttatt gctgttctgc tcctctgtgg attagccgcg atgggactca
    11581 agggggacga tcgtcccgcc ctcaaggtgt ccggactggc atacccttac aagctggata
    11641 gcgtcgatag gtttgaagat aatggttttg atttcgggga aggcgatctg gtggatttgg
    11701 gcaatcacaa gacattttcc tgggatgagg tgctcagaat ggtggatagc caaaaaggcg
    11761 cgaagactga gtacagcctg aactgggatg agatccaaaa cctggtgtat aatatgatct
    11821 ttgtactcag cgccggacaa atctacagat accatctggg cgaaaggttg ctccatgcaa
    11881 tgattcatcc ccagggatcc agcaaagtcc ctctcgggac ccatcacatc cgcctcatgc
    11941 ttttagatcc tacccaggaa gatgtacgcc gggagatgcg cgatcggctg attgcccttc
    12001 tcaaagacga aaacactgaa gccctatggt atctccaaaa aaccagctac ctgtccacca
    12061 atgccaaata tatccaggaa gctgatccca tcttcaaagc cagctaccgc gcctctatcc
    12121 agaatcgcct gaaagaactg atcagcgctg tggaccagac ggactatcag ctcaaagccg
    12181 tgcaaagtta ccccgggcag tgggaacttc tggagcagaa aggcaacaag ctgtacagct
    12241 acactccccg caccctcaat ctgatctgct atcgactggg aaaagacgcc gcccaaaagc
    12301 tcaaatccta tgcccagcaa atccttgacg ctttctagct gatagattgt cctctctgat
    12361 ctctgcagca tcccccaccc acttcggaca aaagcgagct gggtccgcct tgccttggct
    12421 atgctcctga gcagcggctc agtaagggct ctgagcgtcc atcccggact tgctcccggg
    12481 gtttaatgat aatcccggcc agggtttaac agcaaaacag cctcctctcc accttcaccc
    12541 cgcctgggct ccactcatgc caaggcacag gagaggcaaa ggagaggcat aggtggtgta
    12601 aaggaggcta aggggattgt ggcagtttgc tattgacaga atcgccatgc aggaattttg
    12661 gtactctacc attaaactat caaaacatga taacatggag gataaatgac tgataccaaa
    12721 cgcgtttacc tattcggtaa cggacaggcc gaaggaaagg ccgacatgaa gaatctcctc
    12781 ggtggtaagg gagccaacct ggccgaaatg aacctgatcg gcttgccagt gccccccgga
    12841 tttaccatca ccacagacac ttgcaacgaa tacaccaagc ttggcaggga tgctgtcagc
    12901 gcgcttctga agtccgaagt cgaagccgct atccgtcacc tggaaaagct gatgaatatg
    12961 gaatttggtt ccgatgccaa tcccttgttg ctttccgtac gttccggggc ccgggcttcc
    13021 atgcctggca tgatggacac catcctcaac ctcggcatga acgacaaagc cgtgcagggc
    13081 atcatcaaga agaccggcaa cgaacgtttc gcctgggata gctatcgccg cttcgtgcaa
    13141 atgtacggag acgtggtgct gggcctcaaa cccgcgtcca aagaggaaga agatcccttt
    13201 gaagtgatca tccacaagat gaagcacgac aagggcgtga ccaacgatct ggacctcagc
    13261 gccgatgacc tcaaacagct ggtggccctc ttcaaagccg cggtgaaaga aaacaccgga
    13321 cacgactttc ccgaaagccc ctgggaacaa ctctggggag ccatctatgc cgtgtttgac
    13381 agctggaaca acgaccgcgc catcttctat cgtcagctca acaacatccc tggcgaatgg
    13441 ggtaccgcgg taaacgtcca ggccatggtg tttggcaaca tgggcaacag cagcgctacc
    13501 ggagtggcct tcacccgtga cgccgccacc ggagagaacc ttttcaacgg cgaatacctg
    13561 atcaacgctc agggcgaaga cgtggtggcc ggcatccgca ctccccaaca gatcaccaaa
    13621 gaaggttccc agcgctgggc caaactggcg ggagtctcgg aagctgatcg cgtggccaag
    13681 ttccccagtc tggaagaagc catgcccaca gtctatcagg agctctttgc cgctcagcgc
    13741 aagcttgaaa accatgcccg cgatatgcag gacatcgaat tcaccattca ggaaggcaaa
    13801 ctctggatgc tgcagacccg caacggcaaa cgcaccggtt ttgccatggt gagaatcgcc
    13861 atggaaatgc tgcgggacgg catgattgac gatcagaccg cgctcctgcg catggaaccc
    13921 aacaagctcg acgaactctt gcatcccgtc ttctcacgcc agggcctggc agccgcgaaa
    13981 gtgatcacca aaggcctgcc tgcttctcct ggtgcggcta ccggccagat cgtcttcttt
    14041 gcagacgatg ccgaagcctg ggcagctcag ggcaaaaaag tggtgctttg ccgggttgag
    14101 acctctccgg aagacctgcg cggcatgaac gtggcccagg gtattttgac ggcccggggc
    14161 ggcatgacct ctcacgccgc tgtcgtggcg cgcggaatgg gaaaatgctg cgtgagcgga
    14221 gctggagctc tggtgataga ctacaagctt cgcaccatga gtgtggatgg aaacgtctat
    14281 aaagagggcg attggatctc cctcaacggc agcaccggtc aggtgctgga aggcaagatt
    14341 gagaccgtgg agcctgaatt gagcggcgac tttggcgctc tgatggaact ggccgacaag
    14401 catacccgca tgaaagtgcg caccaatgcc gatactccca aagacgcgcg cctggcccgc
    14461 ggttttggag ctcagggcat aggtctttgc cgcaccgaac atatgttctt tgaaggtgac
    14521 cgcatcaaag ccatgcgtga aatgatcctc gccgaggatg aaaccggccg ccgcaaagga
    14581 ctgcacaagc tgctgcccat gcagcgctcc gactttgaag gtatcttcga ggccatggac
    14641 ggcttccccg tcacggtgcg tctgctggat cctcccctcc atgagtttgt ccctcatgag
    14701 gaagaacagc agcaggaact tgccgacgaa ctcggcatct ccctccgcaa agtcaaagaa
    14761 agcgtcgccg ccctgcatga gtttaatccc atgctgggac accgtggctg ccggctggga
    14821 aactcctatc cggaaatcac ggaaatgcag gctcgcgcca ttatcgaagc cgccatcaac
    14881 gtgaccgcca aaggcatcaa agtgctgccg gaaatcatgg taccgctgat cggcaccccg
    14941 gctgaatttg agctccagga agagatcatc cgcgcgaccg ctgaaaaggt cttcgcggaa
    15001 aaagggaccc gcgtggacta cctggtgggc accatgatcg aaatcccccg cgccgctctg
    15061 gtggccgata aaatcgccgt ccgggctgaa ttcttcagct ttggcaccaa cgatctcact
    15121 cagatgacct ttggttactc gcgtgacgat tcaggcgtgt ttatgccggt ttacctggcc
    15181 aaaaacctcc tgaaacacga tcccttccag atcctcgatc aggagggcgt gggtcaactc
    15241 gtggaaatgg gtaccctgag aggccgcatg acccgccctg aactgaaagt gggaatctgc
    15301 ggagaacatg gcggagagcc cagttccgtg gaattctgcc accgcgtggg catgaactac
    15361 gtgagttgtt cacccttccg cgttccgatc gctcgtctgg ccgcggctat ggcagccatc
    15421 cgttaaccaa caacttggtc catataaact ggaagcgccg gagtgaatcc ggcgcttcta
    15481 ttatgttgtt aataaatact tatacaaccc tgagcatttg atctcccgcc atagcaaaga
    15541 ggatgttgct gtcaagctca ccactgggaa cgggatgggg ttctattcta aggtcaacct
    15601 gtctgcgagc gatcatcaac cttacttgtt cttcgaagag gtcctctcca aagtcgggag
    15661 atacgatcag taggtcgata tcgctgtaat catctggttt gccgtccaca taagagccaa
    15721 acaggtacac ggactccacg cggatacgct ggctaaccag ctccacatac cggttgatga
    15781 tttctataag ttctgttcta tctgggatac cagccattgg taaaacctct cgattaaatc
    15841 aagatacttt tttgcataat cctgggtgca ctttttgtag aggcaagctt gacgtccgga
    15901 tagcgggcgt ttatgttgaa aaggctgatt gtatcaagat gggcttgatc ttcagcgctg
    15961 acatccaaac cggcaaggag ggccagacgg tttaggtcat gcagcctggg agcattattc
    16021 ccaacctttt gagaataaac agctttcatc gctttttcca ggactaactg ccccaaaaac
    16081 aagctccagg caaattcccc ggtatcgaac agacgtttca tcgattgaaa gtccatttct
    16141 gccgagtcca gccagtactt gatcaattcc tctttccgca tgcttccatt gtcacaagct
    16201 cgcttaatct gtcaagccga aaaaaaac
//
LOCUS       DAOSFN010000047        15587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3096_length_15587_cov_8.972186, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000047 DAOSFN010000000
VERSION     DAOSFN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 15587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15587
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3096_length_15587_cov_8.972186"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2649
                     /locus_tag="PLO35_04565"
     CDS             <1..2649
                     /locus_tag="PLO35_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH78972.1"
                     /translation="WTGQFYDGTCLAEALMRSRDASAEEARGAIAIYTAAPEQGVAPP
                     MEAQDHTVELLVNGTKQTIGGLCYNGAASMIDLYGEGGVYNFLAWNLFGDASLSLRTK
                     PAQTIAAILPVELPPYYANLDIDAGAPDILVSLSKDKLCVVSGFSGPDGMAHLVMQPY
                     PVPGDRYTLTLSGVDRIPVQRVLNCYQYGTHAVLDLELLPGEQFLEPEIEILKTLKIT
                     NRGDIASGNLSVRLQPDPLENHIYQYDGVSQIASLQPGESVQIQLSYKVHKGTPDLTT
                     VNYSFRIDQTGVSLPCSDVIHAPTIALEDVYRSPQINWTNPGDALDFVYKLRNEGSAG
                     LRDLQAQLSTDDSELIVTQQDERTLNIAPGCSDSLVVSAMVSCACPIYQTLSATLNLE
                     ADNAIGEFWHQWKVTDPAHTVESFESGDLQTFPWVYQSEHWNMSNHSLDGTKSLVSEA
                     SAADSIWLALGFHSNQTGELSFRYDLYRSAACLDGWTFWIDDNQVNELENSNWWNIKR
                     FSLSEGDHTLQWVGRKDPSMDVFASSFCLDLIEFPEGTLFDNARLETDVSQVHITLSP
                     GEIRDIPVILRSLDGKDIAYAAVLKRAEVSKRAGTPYLECNKTTFQAGSEELCLFSLY
                     NSDPVMQIKALSITLPERVLALQASSFIMSGQTSLPFTGSLGSYSSLDWSSEIGSLAD
                     SLRTAVRLALDASLEDLALPYRIETVDGRTFEDCINLERSDDQDECLVLSSDEGMVGD
                     LETRAIILRANQNLLGPEPASYYLDIYYNGSHLNRVPVGISYDSNPPGFYDEPRLSLY
                     PNPCRHGSTFAYGIPAPGKVQLVIYNLRGQRVRTVVNGNLEKGYYRSFWDAKDDQGQR
                     VGSGVYYCRLSTPAGGQKLIRCVVMK"
     gene            2757..4847
                     /gene="ovoA"
                     /locus_tag="PLO35_04570"
     CDS             2757..4847
                     /gene="ovoA"
                     /locus_tag="PLO35_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HOH78973.1"
                     /translation="MEAIKGIRTLNLKEPDPALLRERVRQYFNQTYTIEERLYEQLIG
                     DDTFYLRADPLRHPLVFYLGHTAVFYVNKLNIGKVISQRINPRFESMFAVGVDEMSWD
                     DLNESHYDWPPIPEVRAYRDEVRALVDNLISTLPLDEKGIEWDSPWWAIVMGIEHERI
                     HLETSSVLIRQLPLERLRQLDFWAPCEPSGTAPDNELLPVQGGKVGLGKTDEDPWYGW
                     DNEYGHAAYEVEDFSASKYLVSNAEFLKFVQAGGYEKQRWWTPEGWSWKSYKNAGHPQ
                     FWINDKGVWKLRLVASVISMPWNWPVEVNYLEAKAFCNWMTEQSGKAIRLPMEEEWYR
                     LRDLCIDTDQPLWNKAPGNINLEAYNSSCPVDIYENKGFYDVIGNVWQWTETPIYAYP
                     GFKIHPIYDDFSVPTFDGRHNLIKGGSWISTGNEALRNSRYAFRRHFMQHAGFRYIQS
                     EAPVVEHGDFYENDAGIINWCDASWGEPPLGDRHYSLGLIDGLRGLEFTGGKALHIGC
                     KTGRGTFELARHFDSVVGLDFTARLIRMATDMKEKGYIRYLRNDEGQNQSYIESRLES
                     FELSAEAAKVEFWQADPSNLIAKFDGYDFILAENVLAEAINPGRFLKEVISRLVPGGV
                     LAIADHYERSDGQALGGFRKDGEPFSHLEGLTEALTGTMAPLQGPKDVWQPLRSSERS
                     YEYRKLQLTLWRKL"
     gene            4851..6818
                     /locus_tag="PLO35_04575"
     CDS             4851..6818
                     /locus_tag="PLO35_04575"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOH78974.1"
                     /translation="MKRLALLLALLALALNSQGELVQIGSGSLVNQGLPIEPLSIYGY
                     SQQLYLASEIGGAGPITELSFQYSVDYNEFYDRNKALRIWLGHTQRNQITAWVPVDSL
                     SLYYDAIVPLTAFSGGLPGQGWMSITLDDPFLYDGSSNLLIAVMEDSPGHSGSTDEFL
                     NSASTTSRGIVCRSEVPLDPNNLPASGVYVRQAFPNLRLEIAITHLTPYLPSPANAAE
                     LVPIDTSFDWSSDAGLFDFWFGTGPEDLDLVQSGLAESAFFFTEPLGLLSTYYWQVIG
                     WHEGLSYPSPVWSFSTAGELLSPPQNFQAWWQDNEVRMSWEAPISGTVQDYLVFRNEQ
                     QLGICQATVYQDPNVAQGAVYVYYVKARNSLGQTSLPSNSGSVHIPSTEPNLILWKDF
                     ETEALWSSEIPGWTILDLDGSPTWSWDFVDFPNEGTACGWMVFAPGLTVPPLSEFQAH
                     SGARMLMAPDALNPPDDNWLISQPLYLGAGPSLSFWARSATADYGLERLRVLISSTGS
                     EPADFLPLHTQAWLAVPAAWTQYQYDLTAWAGQTVRFAWQSVSVDAMALFLDDLKFYG
                     EGGYTASDDPVVAVPCFRVWPNPSRGVFKASLEGDAFDLCVYDIRGRKICNHKALREF
                     DGSALDLASGIYILKASQRGKSYTARLVIIK"
     gene            6979..>9464
                     /locus_tag="PLO35_04580"
     CDS             6979..>9464
                     /locus_tag="PLO35_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH78975.1"
                     /translation="MRPSPHSQGINSFIEELLSLPHYYENIVARQELPEKQAQWSDWP
                     QAVQEGLKATLMELGYTRLYTHQAQAIEAALQGRDLVISTGVASGKSLCYQVPILQDQ
                     LIGSKTRALMLFPTKALAQDQRQKTQALLLALTQKLPQAPKLSCGIYDGDTPSEARRQ
                     VRSTARIIFSNPDMLHAAILTNQSLWASFFAGLRYVVIDEVHMYRGVFGSHFANVIRR
                     LKRICSIYGSSPQFICTSATVANAFQLASDILERPVKLIDQDGSPHGERLFCLLNPPM
                     ISKQLGIRRSSVIESSSLAKRWLKTDGQALLFSISRRSVEILFMYLQNRPDLMSRARS
                     YRSGYLPEERRKIEKELRDREIGLVISTNALELGIDIGGLDAVFMNGYPGTISATRQQ
                     AGRAGRIGNSSLCVLVASSNPLDQYICQHPDYLFDNNPEQALIAPDHPEILRQQLLCA
                     VHELALLDGEGFGSLGPEHIFPYLKVLEEDGLIHRQRDRWVGKLEAWPAGEVSLRNIS
                     SQMQINCKDQLIGYVDAASALWMTHPGAIYLDRGEMWMVSDLDLEHGKVEVEPVRVNY
                     LTQTTRQSEIELLSLLRREHNHLGSKCFGNVKVTSLITGFKKVRFFTQELVGYEELQL
                     PPSVLETQAWWFSLSQQVVQRVREQGLWRNDPKDYGRDWNRTKDSIRSRDRFRCGHCG
                     AAESGKAFDVHHITPLRMFTDLQAANHPDNLITLCPRCHRLAEEAVRIQSGLAGLAYL
                     LVNLAPFYVMCDRKDIDVIQEERSELAEGDPVIAIYDNIPGGIGLSRKLYELQDRILK
                     EALELAKACPCEDGCPACVGPVAENGAGAK"
     assembly_gap    9465..9564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9565..>11983
                     /locus_tag="PLO35_04585"
     CDS             <9565..>11983
                     /locus_tag="PLO35_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH78976.1"
                     /translation="WPTESYRYLGTQFQRGYQIAVINVFPYKYNPVTRELLVYEDISL
                     SLHASPDRDLSSRQANMLRRDANALRELAEMVVNPSEANSYAGAERFRTHVPQSRLID
                     LSTPKQMIVITDPTRVDWFTDYVQWREANGVSTGIFTTQDIYAQYTGIDNAAKVRAFI
                     IDAYQSWAATSTPLEYVILGGDDEIVPERGAYGQVGDTVDTRMPVDIYFSNLDGTWDA
                     NNNQIYGEILDDTDMVPEVHIGRFPAELQSEFQNMFRKIQYYVQNTTFSNNIALMFGE
                     NLNWNPVTWGGDYKDDVATHIPETYELRTLYQRDGTYNENLVWNAINDGANVMNHMGH
                     ANETFLMGQGNNTIEQLQNTEYGVLYTQGCYPAAFDQRTSGDGECIAEHMVTTAGGLF
                     SFIGNTRYGWYMPGGINGASQFYDRQYFIGLYETLNTELGKALTYSRAQNLNAALTND
                     VMRWCYYEVVLFGDPSISVKYPDPSLPLLSLESYEITDEDGDGDGSFNPGESLRIYPR
                     VRNQDGWAAAHNVSVTVTGMPYGVQLLGPCISIADLAPGALSSPDLYISFQLPYDLPY
                     GEYNLKLSIDARHPVTQLSIGVRQTTATFSITLIDNRFPWDCENSTHSAPIVYDFNND
                     DDLDIMYLDVFGETYLIGTDGEQFGGFDAPSEQNVMRSSAMGDINNDGNPDLVFSSRT
                     GTVYASTLSGTPIFNYDSGSSFLFTPVIADIDGDGTNEVLVHSLDKRLFAFRANGTQV
                     PGFPVSLTSTFPSELAAADLDNDGACEIILGTQNGQLHVIRGNGLAQTGFPVNVGGPV
                     TGSPLVLE"
     assembly_gap    11984..12083
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12084..12581
                     /locus_tag="PLO35_04590"
     CDS             <12084..12581
                     /locus_tag="PLO35_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH78977.1"
                     /translation="TSFNGNTPATLTDLDGDGILRLISGYSTGILVVNLRRPVTAKMP
                     WTVYRGGLMRQGSYAATGYVDNPNQTIPPLRDQLLQNYPNPFNPSTSISFSLSKASHA
                     SLRIYNLKGQLVRTLLDGQLSQGSHTLVWDGRDSSGTSVASGVYLYRLETPGSSFGKR
                     MLLMK"
     gene            12680..>13551
                     /locus_tag="PLO35_04595"
     CDS             12680..>13551
                     /locus_tag="PLO35_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOH78978.1"
                     /translation="MRPTLYLIDGTALLYRSHFAFIANPLVNSKGQHTSAIFGVINSF
                     LRLLELKHADHILISFDRKAPTFRHELSEAYKANRPPMPDDLISQIEPVKQFFELIGL
                     PEISLDGYEADDVLATLAERYKTDYDVVLVTSDKDYSQLVEAGVSIWDPAKDVTLDSD
                     AVFTKFGVRPQQFIDYLALVGDASDNIPGVKGIGPKGAVDLLTQFGSLDNIYTALEQV
                     PEKYRKKLEADKDAALLSRTLATIIRDVPIEPVHLKPFDASSLVKAADLLSAYELLNL
                     KRRMESRYSKPAIK"
     assembly_gap    13552..13651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13652..15427
                     /gene="polA"
                     /locus_tag="PLO35_04600"
     CDS             <13652..15427
                     /gene="polA"
                     /locus_tag="PLO35_04600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOH78979.1"
                     /translation="TPANFPELLSALQSASEISIDTETDSIDPIQANLVGISLCYSAQ
                     NAWYLPLGHQLQDNLLLSEVVPALSRALDGKLLSGHNLKYDLIVLERHGWKISNPIFD
                     TMLAAYVLEPGTNQYSLDACAARELHHEMIPITAIIGSGKNQITFDLADPVKAAEYAA
                     EDAWAVFRLTPIYRRRLEFTGLHPLYQDIELPLIRVLKRMEQNGVALDSSILRELSAS
                     INTELKRLTDLIYDYAGYQFNLNSTQQLAKLLFEEKGLPTKKKTKSGFSTDSAVLEEL
                     ADDHEIARLLIQYRMLSKLESTYVNTLPKLINPATQRVHSSFNQTVASTGRLSSSNPN
                     LQNIPIRTELGRQVRKAFVASAPNRVILAADYSQIELRLLALFSKDEVLLQAFRDGLD
                     IHRQTAAYIHNKPLEEVSSDERRGAKTINFGLLYGMGQKKLSRELGISLADAKEMIRN
                     YFERFPTIRDYIRSSIDAARQNRYCETLFGRKLYLPNINSNNQGLKSEAERVSVNMPI
                     QGSAADLIKIAMLDIHAQIKAEDEIRMVLQVHDELVFDVLADRVDEASHIIRSAMENA
                     LPQQYRSIIHLQTDIGVGRDWYESH"
BASE COUNT         3431 a         4442 c         4159 g         3255 t
ORIGIN      
        1 tggactggcc agttttatga cggcacctgt ctggccgaag ccctgatgcg aagccgggat
       61 gccagtgctg aagaagcccg gggagcgatt gccatctata ccgcggctcc cgaacagggc
      121 gttgctccac ccatggaagc tcaggaccac acagtggaac tgctggtgaa tggaaccaaa
      181 cagacgatcg gaggtctctg ctacaatggg gccgccagca tgatcgatct ctatggcgag
      241 ggaggagtgt ataacttcct ggcctggaac ctgtttgggg acgccagcct aagcctgagg
      301 accaagcccg cccagacgat agctgccatc ctgccagtgg aattgcctcc ctattatgcc
      361 aatctcgata tcgacgccgg cgcgcctgat atcctggttt ccctctccaa agacaagcta
      421 tgcgtggtga gcggtttctc cggtccggac ggcatggccc atctggtcat gcagccctat
      481 cccgttccag gcgatagata tacgctcaca ctgagcggag tggaccgcat cccggtgcag
      541 agggtgttaa actgctatca gtatggaacc cacgcggttt tggacctgga actgctgccg
      601 ggtgagcagt ttctggagcc ggagattgag attctgaaaa cgctcaaaat caccaataga
      661 ggcgatattg ccagtggcaa cctgagcgtt cgcctgcagc ccgatcctct ggaaaatcat
      721 atttaccagt atgatggagt gagccaaatc gcgtctcttc agcccggcga atccgtccaa
      781 atccagttat cctacaaggt tcacaaaggc acccctgatc tgaccacggt caattactct
      841 ttccggattg atcagacggg agtcagtttg ccctgctcgg atgtcattca cgctcccacg
      901 atagcgctgg aggacgttta caggtctcct cagatcaatt ggaccaatcc gggagacgcg
      961 cttgactttg tctataagct ccgcaatgag ggcagcgctg gcttgcggga tttacaagcg
     1021 cagcttagca ctgatgatag tgaattgatt gtcactcaac aggatgagcg cactctgaac
     1081 atagctccgg gctgcagcga ttctctggtg gtaagcgcga tggtttcatg cgcttgcccc
     1141 atctatcaga ccctgagcgc gacgctcaat ctcgaggctg acaacgccat tggggagttt
     1201 tggcaccagt ggaaggtgac cgatcccgct catacggtgg agagctttga atctggcgat
     1261 ctgcagacct tcccctgggt ctatcaatcg gagcattgga acatgagcaa tcacagcctg
     1321 gatggcacaa aaagtctggt gtctgaggcc agcgcggctg attccatctg gctggccctg
     1381 ggtttccaca gcaaccagac gggagagctc agcttccgtt atgatcttta ccgctccgcg
     1441 gcatgtctgg acggctggac tttctggata gatgataatc aagtgaacga gctggaaaac
     1501 agcaattggt ggaatatcaa gcgtttctct ctctccgagg gggatcacac tctccagtgg
     1561 gtgggcagga aagacccttc catggatgta tttgccagct ctttctgcct ggacctgatc
     1621 gagtttccgg agggtactct gtttgacaac gccaggctgg aaacagatgt ttctcaggtg
     1681 cacatcactc tgtctcccgg tgagatacgt gatattccag tgatcctgcg ctctctggat
     1741 ggcaaggata tcgcttacgc ggcggtactg aagagagcgg aagtctcaaa acgggctgga
     1801 accccttatt tggaatgcaa caagacgact ttccaggctg ggagcgagga actttgcttg
     1861 tttagcctct ataactccga tcctgtcatg cagatcaaag ctttgagcat caccctgccg
     1921 gagagggtat tggctttgca ggccagtagc ttcatcatgt ccgggcagac ctctttgccc
     1981 ttcactggca gcctgggcag ctactcgagc cttgattggt ccagtgaaat cggcagcctg
     2041 gcagacagcc tcagaacggc tgtcagattg gctctggatg ccagtctgga ggatctggcg
     2101 ctgccctatc ggatcgagac tgtggacggg cggacctttg aagactgcat aaatctggag
     2161 cgctctgatg accaggacga gtgcctggtc ctgagctctg atgagggaat ggtgggtgat
     2221 ctggagaccc gggcgattat cctgagggcc aatcaaaacc tgctggggcc agagccggcc
     2281 tcttattatc tggatatcta ctacaacgga tcgcatctca atcgagtgcc cgtcgggata
     2341 agctatgaca gcaatccccc cggtttctat gatgagccgc ggctgagcct ctatcccaat
     2401 ccctgtcgcc acggcagcac ctttgcctat ggcatcccgg cccctggaaa ggtgcaattg
     2461 gtgatctaca acctccgggg ccagagagtg agaactgtgg tgaatggcaa tctggagaag
     2521 ggttactacc gcagcttttg ggatgccaaa gatgatcagg gccagcgtgt gggcagtgga
     2581 gtctattatt gccgcctcag cactccagct ggcggacaga agctaatccg ctgtgtggtg
     2641 atgaaatgag ttcctggcac gggatgcgga taatttgtgc ttgacagacc cgtccgcctg
     2701 ggccagattg tcgaaagagc tatttcacag ccagccatcc agcaaggagc acagccatgg
     2761 aagccatcaa agggatccgc accctgaacc tcaaagagcc ggaccccgcc ctcctccgag
     2821 agcgggtaag gcaatacttt aaccagactt acacgatcga agagcggctc tacgaacagc
     2881 tgatcgggga tgatactttt tatctgaggg cagatcccct gcgtcatccg ctggtctttt
     2941 atctgggcca cacggcggtc ttttacgtca acaaactcaa catcggcaag gtgatcagcc
     3001 aaaggatcaa tccccgcttt gaatcgatgt ttgccgtggg cgtggatgaa atgagctggg
     3061 acgacctcaa cgagtcccac tacgactggc ctcccattcc agaggtgagg gcctacagag
     3121 acgaggtgcg tgccctggtg gacaatctca tcagcaccct accgctggat gaaaaaggga
     3181 tcgagtggga cagcccctgg tgggccatcg tcatgggcat cgagcatgaa cgcattcatc
     3241 tggagacctc atcggtgctg attcgtcagt tgccgctgga aagactgcga cagctggatt
     3301 tctgggctcc ctgcgagcct tcgggcaccg caccagataa tgaactgctg ccggtgcagg
     3361 gtggaaaggt aggcctgggc aagacagatg aggatccctg gtatggctgg gataacgagt
     3421 atggccatgc cgcgtatgag gttgaggact tctccgcgtc caaatatctg gtctccaatg
     3481 ccgagtttct caagtttgtc caagcaggcg gatatgagaa gcagcgctgg tggactcctg
     3541 agggctggag ctggaagagc tataaaaacg cgggacatcc gcagttttgg ataaacgaca
     3601 agggggtctg gaagctcagg ctggtggcct ccgtcatttc catgccctgg aactggccag
     3661 tggaggtcaa ctatctggag gccaaggcct tctgcaactg gatgaccgag cagagtggaa
     3721 aagctatcag actgcctatg gaggaagagt ggtaccggct cagagacctc tgcatcgata
     3781 ccgatcagcc cctctggaac aaggcccccg gcaatatcaa tctggaggct tacaactcct
     3841 cctgtccggt ggatatctat gagaacaaag gcttttacga tgtgatcggc aatgtctggc
     3901 aatggaccga aacccccatc tatgcctatc caggtttcaa gatacatccc atctacgacg
     3961 actttagcgt gcccaccttc gatggccgcc acaacctcat caaaggtggc tcctggattt
     4021 ccaccgggaa tgaagctctc aggaactccc gctatgcctt ccggcgtcat tttatgcagc
     4081 atgccggctt ccggtatatc cagagtgaag ctcctgtggt ggagcacggc gatttttatg
     4141 agaacgatgc cggtatcatc aattggtgcg acgcctcctg gggagagccc cctctgggag
     4201 acaggcatta tagcctgggg ctcatcgatg ggctcagggg ccttgaattc accggtggaa
     4261 aagccctgca tattggctgc aagacgggcc gcggcacttt tgaactggcc cggcatttcg
     4321 acagcgtggt ggggctggat ttcaccgccc gcctgatccg catggccaca gacatgaagg
     4381 aaaagggcta catccgctat ctccgcaacg atgaaggaca gaaccagagc tacatcgaaa
     4441 gccggctgga gagctttgaa ttgagcgctg aggctgccaa agtggagttc tggcaggccg
     4501 atccctccaa tctgatcgcc aagtttgacg gctatgactt tatcctggcg gaaaacgtgt
     4561 tggctgaggc gatcaatcca ggccgcttcc tcaaagaagt gatctccaga ctggtgccgg
     4621 gaggagtgct ggccattgcg gatcattatg aacgctccga tggccaggct ctgggaggct
     4681 ttcgtaaaga tggagagcct ttcagccatc tggaagggct caccgaagcg ctgacgggaa
     4741 ccatggcacc gctgcaaggg ccgaaagatg tctggcagcc cctgcgttcc tctgaaagaa
     4801 gctatgaata tcgcaaactg cagctcacgc tctggcgcaa actatagcag atgaaacgtt
     4861 tggcactgtt gctggctttg ctggccctcg ctttgaatag ccagggagaa ctggtgcaga
     4921 tcggctctgg cagtttagtg aatcagggat tgcccatcga gcctctatcc atctatggct
     4981 atagccagca gctgtatctg gcctccgaaa tcgggggcgc gggacccatc actgagctta
     5041 gctttcaata cagcgtggac tataacgagt tttatgatcg caacaaagca ctgagaatct
     5101 ggctggggca tacccagagg aaccagatca cagcctgggt tcctgtggac agcctgagcc
     5161 tctactacga tgcgatagtg ccacttacgg cattctcggg aggcttgcca gggcaaggat
     5221 ggatgagcat cacgctggat gaccccttcc tctatgacgg cagctctaat ctgttgattg
     5281 ccgtaatgga agacagccct gggcactctg gcagcaccga cgagtttctc aactcagcca
     5341 gcaccacttc aaggggcatc gtttgtcgca gtgaagtgcc tttggatccc aataaccttc
     5401 ccgcttcggg agtttatgtc cgccaggctt ttcccaatct gcgcctcgag atcgccatca
     5461 cgcatctgac accgtatctt ccatctccag ccaatgccgc agagcttgtt cccatcgata
     5521 cgagctttga ctggagcagc gacgcgggac tctttgattt ctggtttggg accgggcctg
     5581 aagatcttga tctggttcaa tccggcctcg ctgagagcgc tttctttttc accgagcccc
     5641 tgggcctgct gagcacatat tactggcagg taatcggttg gcatgagggg ctgtcctatc
     5701 ccagccccgt ctggagtttc agcacggccg gggagctcct gagccctcct cagaacttcc
     5761 aggcctggtg gcaggacaac gaggtccgca tgagttggga agccccgatc agcggaacgg
     5821 tgcaggatta tctggtgttt cgcaacgaac agcagttggg tatttgccag gctacggttt
     5881 atcaggatcc caatgtggct cagggagcgg tctatgttta ctatgtgaaa gcccgtaatt
     5941 cactggggca aacatctttg ccatcaaaca gcggcagtgt gcacatccca tccacggagc
     6001 cgaatctgat cctctggaag gattttgaaa ctgaagcgct gtggagctct gagattccag
     6061 gctggaccat cctcgatctg gacggcagtc ccacctggag ctgggatttc gtggattttc
     6121 ccaatgaggg aacggcctgt ggctggatgg tcttcgctcc cggactcacc gttcctcctc
     6181 tcagcgagtt tcaagcccac agcggagccc ggatgttgat ggccccggac gctcttaacc
     6241 ctccagacga caactggttg atctctcagc ctctgtattt gggagcgggg cccagcctca
     6301 gcttttgggc ccgtagcgcc actgcggatt acggactgga acggttgaga gtgctgatct
     6361 cgtccacggg gagcgaaccg gccgactttc tgcccctgca cacgcaagcc tggcttgccg
     6421 ttccggcggc ttggacccaa tatcagtatg atctgacagc ctgggctgga cagactgtga
     6481 gatttgcctg gcaaagcgtg tcggtagatg ccatggcgct gtttttggat gatctcaagt
     6541 tctatgggga agggggatat accgcgtccg atgatcctgt cgtggctgtt ccgtgtttca
     6601 gagtatggcc caatccctcc cggggagtgt ttaaggccag tctggaaggg gacgcgtttg
     6661 acctgtgcgt ctatgacatc agaggcagga agatatgcaa tcacaaagcc ttgagggagt
     6721 ttgacggcag cgcgctcgat ctggcctccg ggatctatat cctgaaggcg tctcagagag
     6781 gaaagtcata tactgccagg ctggtgataa tcaaatgaaa tccagcactt tagaaccgaa
     6841 accatcctca tttgtatatc agcataacac cctctccaat aataagaaac atctctgtac
     6901 ttctctctgt gacctccgtt acctctgtag tgaaaaagca attctctgta gctctgaagt
     6961 ggaaaagcaa ttagagcgat gagaccatca cctcactctc aaggtataaa cagcttcatc
     7021 gaagagctcc tgagcctccc tcactactat gagaacatcg tagcccggca ggagcttcct
     7081 gaaaagcagg cccagtggtc ggactggcct caggccgttc aggaagggct caaagccacc
     7141 ctgatggaac tgggctacac caggctttac actcatcagg cgcaagccat tgaagccgct
     7201 ctgcaaggcc gcgacctggt gatcagcact ggagtggcca gtggcaaaag tctttgctat
     7261 caggtgccca tcctccagga ccagctcatc ggctccaaaa ccagagcgct catgcttttt
     7321 cccaccaaag ccctggcgca ggaccaacgt caaaagaccc aggcattgct gctggccttg
     7381 acccaaaaac tgccccaggc ccccaaattg agctgtggca tctatgatgg agacactccc
     7441 tctgaggcca gacgtcaggt gcgctccacg gcccgcatca tcttttccaa tccggacatg
     7501 ctgcatgccg ccatcctcac caatcaaagc ctctgggcct ccttcttcgc ggggctcaga
     7561 tatgtggtga tcgatgaggt gcacatgtac cgcggtgtct ttggctccca ttttgccaac
     7621 gtgatcagga ggctcaagcg gatctgctcc atctatggct cttcccctca gttcatctgc
     7681 acttccgcca cggtggccaa tgcgtttcaa ctggcttcag acatcctgga gcgtcccgtg
     7741 aagctcatcg accaggatgg ctcgcctcat ggagagcggc ttttctgcct gctcaatcct
     7801 cccatgatca gcaaacaact ggggatccgg cgcagcagcg tgatcgaaag ctcttctctg
     7861 gccaagcgtt ggctcaaaac agacggccag gccctgctgt tcagcatttc aaggcgcagc
     7921 gtggagatcc tcttcatgta tctgcaaaac cgtcctgatt tgatgtcccg ggcgcgcagt
     7981 tatcgtagcg gctaccttcc ggaagaacgg cgcaagatag aaaaggaact gcgggataga
     8041 gagataggcc tggtgatcag caccaacgct ctggaactgg gcatcgatat cggtggcctc
     8101 gatgccgtct ttatgaatgg ctatccgggc acgatttccg ccacccgtca acaagccggc
     8161 cgggctggca ggatcggtaa ttcctcgctt tgcgtgctgg tggcctcctc caatcctttg
     8221 gaccaataca tctgccagca tcccgactac ctttttgaca acaatcccga acaggccctc
     8281 atcgctccgg accatccgga aatcctcagg cagcagctcc tctgcgcggt tcatgagctg
     8341 gcgctgctgg atggggaagg ttttggcagc ctgggtcctg agcacatctt cccctatctc
     8401 aaagtgctgg aagaggatgg cctcatccat cggcagcggg atcgctgggt gggcaagctg
     8461 gaagcctggc cggcgggaga agtctcgctg cgcaacatca gcagccagat gcagatcaac
     8521 tgcaaagacc agctgattgg ctatgtggat gccgccagcg cgctctggat gacccatccg
     8581 ggagctatct atctggatcg cggagagatg tggatggtgt ccgatctcga tctggaacac
     8641 ggcaaagtgg aagtggaacc cgtacgggtc aactatctga cccagaccac ccggcagagc
     8701 gaaatcgagc tgctgtcgct cctgcgcaga gagcacaacc acctgggcag caaatgcttt
     8761 ggcaacgtga aggtcaccag cctgatcacc ggattcaaga aggtgagatt ctttacccag
     8821 gaactggtgg gctatgaaga actgcagttg ccaccctccg tgttggaaac tcaggcctgg
     8881 tggttttcac tctctcagca agtggttcag agagtaaggg aacagggcct ctggcgcaac
     8941 gatcccaaag actatggcag agactggaac cgcaccaaag acagcatccg cagccgcgac
     9001 cgttttcgtt gtggtcactg cggcgcggct gagagcggta aggcctttga cgttcatcat
     9061 ataacccctc tgcggatgtt cactgatctg caggcggcca accatcccga caacctcatc
     9121 accctctgtc cccgctgtca cagactggct gaagaggcgg tgcgtatcca gagcggtctg
     9181 gccggcctgg cctacctgct ggtaaatctg gcccccttct atgtgatgtg cgaccgcaag
     9241 gatatcgacg tgatccagga agaacgcagc gaactggcag agggcgatcc cgtgatcgcc
     9301 atttatgaca acattcccgg ggggataggc ctctcccgca agctctatga actgcaggac
     9361 cgtatcctga aagaggcttt ggaattggcg aaagcgtgtc cctgtgagga tggctgccct
     9421 gcctgtgtgg gccccgtggc ggaaaacgga gcgggggcca agcannnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnntggccc acagagtctt accgctatct gggcacccag
     9601 ttccagcgcg gctatcagat cgccgtgatc aacgtctttc cttataaata caaccctgtc
     9661 actagagaac tgctggtcta tgaagatatt tcgctgagcc tgcatgccag tcccgatcgc
     9721 gatctcagca gccgtcaggc caatatgctc aggcgggatg ccaacgcgct cagagagctg
     9781 gccgaaatgg tggtaaatcc atctgaggca aacagctacg ccggggcaga gcgcttccgg
     9841 acgcatgtgc cccagagccg tctgatcgat ctttccactc ccaagcagat gatcgtgatc
     9901 acagacccca cccgggtgga ctggttcacg gattacgtcc agtggcgcga ggccaatgga
     9961 gtgagtaccg gcatcttcac cactcaggac atctatgccc aatataccgg catcgacaac
    10021 gccgccaaag tgcgggcttt catcatcgat gcctatcaga gctgggcggc cacttccacc
    10081 ccgctggaat acgtgatctt aggaggcgat gacgagatcg ttccggaacg cggagcttat
    10141 ggtcaggtgg gcgacaccgt ggataccaga atgccggtgg acatctattt cagcaatctg
    10201 gatggcacct gggacgccaa caacaaccag atctacggcg aaatcctcga cgacaccgat
    10261 atggttcccg aagtgcacat tggccgtttt cccgccgagc tccaaagcga gttccaaaac
    10321 atgttccgca aaatccaata ctacgtgcaa aacactacct tcagcaacaa catcgctctc
    10381 atgtttggcg aaaacctcaa ttggaacccc gtcacctggg gcggagacta taaagacgac
    10441 gtggccactc atatcccgga gacctacgag ctgcgcaccc tctaccagag agatgggacc
    10501 tacaacgaaa acctcgtctg gaatgccatc aacgacggcg cgaacgtgat gaaccacatg
    10561 ggccacgcca acgagacctt cctgatggga cagggcaaca ataccatcga acagcttcaa
    10621 aacacggaat atggcgttct ttacacccag ggctgctatc ccgccgcttt cgatcaacgc
    10681 accagcggag acggtgaatg catcgccgag cacatggtca ccacggcagg cgggctgttc
    10741 agcttcatcg gcaacacccg ttacggatgg tatatgccgg gcggcatcaa cggcgcgtcg
    10801 cagttctatg accgccagta ctttatcggc ctgtatgaga ccctcaacac cgagctcggc
    10861 aaagccctca cctattcccg ggcccaaaac ctcaacgccg cgctcacaaa cgacgtgatg
    10921 cgctggtgct attatgaggt ggtgctgttt ggcgatccct ccatcagcgt caaatatccc
    10981 gatccctccc tgccgctcct cagcctcgaa agctatgaga tcaccgatga ggatggcgac
    11041 ggagacggca gtttcaatcc cggagaatcc ctcaggatct atccccgcgt ccgcaatcag
    11101 gatggctggg cagccgccca caacgtgagt gtcaccgtca ccgggatgcc ttatggagtg
    11161 cagcttttgg ggccctgcat cagtattgcc gatctggccc cgggagcgct ttcctcgcct
    11221 gatctctata tcagtttcca gctgccctac gatctgccct atggagaata caacctcaag
    11281 ctcagcatcg atgcccgcca tcccgtcacg cagctctcta tcggcgtgag gcagaccact
    11341 gccacttttt ccattactct gatcgacaac cgcttcccct gggattgcga aaacagcacc
    11401 cacagcgctc ccatcgtgta tgatttcaat aacgacgacg acctggacat catgtatctg
    11461 gacgtctttg gagagactta tctgatcggg acggacggag agcagtttgg cggcttcgac
    11521 gctccaagtg agcaaaacgt gatgcgcagc tccgccatgg gagacatcaa caacgacggc
    11581 aatcccgatc tcgtgttcag cagccgcacc gggacagtct atgccagcac tctgagcggg
    11641 acgcctatct tcaactacga cagcggctcc agctttcttt tcactcccgt gattgccgat
    11701 atcgatggcg acgggaccaa cgaagtgctg gtccactcgc tggataaacg gctctttgcc
    11761 ttcagggcaa acggtactca ggtgccggga tttcctgtct cgctcacatc caccttcccc
    11821 agtgaactgg ctgccgccga tctggataat gacggggcct gtgagattat cctgggcacc
    11881 caaaacggcc agttgcacgt gatcagaggc aacggcctgg cccagactgg cttccccgtc
    11941 aatgtgggag gcccggtcac cggttcgccg ctggtgctgg aaannnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnaccagct tcaacggcaa cacccccgcc accctgacag
    12121 acctggacgg cgatggcatc ctgagactga tctcgggtta ttccaccggc attctggtgg
    12181 tcaatctgag gcgtccggtc accgctaaga tgccctggac cgtctatcgg ggcggattga
    12241 tgaggcaggg ttcctatgcc gccaccggct atgtggacaa ccccaatcag accatcccac
    12301 ccctgcggga ccaattgctt cagaattatc ccaatccttt caaccccagc accagcatca
    12361 gtttcagcct cagcaaagcc agccacgcca gcctcaggat ctataatctc aagggccaac
    12421 tggtgcggac tctgctggac ggccaactca gccagggcag ccataccctc gtctgggatg
    12481 gacgcgacag cagcggaaca tccgtggcca gtggagttta tctctaccgt ctggaaactc
    12541 ccggaagcag ttttggcaag cggatgctgc tgatgaaatg atcctgacta atggagtggc
    12601 agacgtcctc gtctgccgtg aagagagtta acagagctca aacggggacg tttgcggttc
    12661 cataagatta ggaggcctcg tgagacccac cctctatctg atcgacggta cggccctgct
    12721 ctaccgttcc cacttcgcct ttatcgccaa cccgctggtg aattccaagg gccagcacac
    12781 cagcgctatc tttggcgtga tcaacagctt tctgaggctg ctggaactca aacacgcgga
    12841 tcacatcctc atcagcttcg accgcaaggc ccccaccttt cgtcacgagc tcagcgaggc
    12901 ctacaaagct aaccgtcctc ccatgccgga tgacctgatc tctcagatcg agccggtgaa
    12961 gcagttcttt gagctcatcg gtctgccgga aatctctctg gacggctacg aggcggatga
    13021 cgtcctcgct accctggcgg agcgctataa gacagactac gacgtggtgc tggtgacctc
    13081 agacaaggat tattcccaac tggtggaagc gggtgtgagc atctgggacc ccgccaagga
    13141 cgtcaccctt gattcggacg ccgtctttac caagtttggt gtgcggcctc agcagtttat
    13201 cgactatctg gccctggtgg gtgacgcgtc agacaatatt cccggggtga aaggcatcgg
    13261 acccaaaggc gcggtggacc tcctcaccca gtttggcagc ctggacaata tctacaccgc
    13321 tctggaacag gtgccagaga agtatcgcaa aaagctggaa gcggataaag acgcggcctt
    13381 gctttcccgc accctggcca ccatcatcag agacgtaccg atcgagccag tccatctgaa
    13441 acctttcgat gcctcctccc tggtcaaagc cgccgatctt ttgagcgcgt atgaattgct
    13501 caacctcaag cgccgcatgg agtcacgcta ttccaaaccg gccatcaaag annnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naccccagcc aacttccctg agctgctcag
    13681 cgcgcttcaa tccgcctcgg aaatcagcat cgacaccgag accgactcca tcgatcccat
    13741 ccaggccaat ctggtgggga tttcgctctg ctacagcgct cagaatgcct ggtatctgcc
    13801 attggggcac caactccagg acaacctcct tttaagcgag gtggtcccgg ctttgtccag
    13861 agctctggat ggcaagctgc tcagcgggca caacctgaag tatgacctca tcgtgctgga
    13921 gcggcacggc tggaagatca gcaaccccat tttcgacacc atgctggccg cctatgtgct
    13981 tgagccgggg acaaatcagt attctctgga tgcctgcgcg gcgcgggaac tgcatcacga
    14041 gatgattccc atcacggcca tcatcggcag cggtaaaaac cagatcacct tcgatctggc
    14101 cgatcccgtc aaggcggccg aatatgccgc tgaagatgca tgggcggttt tcagactgac
    14161 ccccatctac agacgccgcc tggaattcac cggcctccac ccactctatc aggatatcga
    14221 gctcccgctc atccgggtgc tcaaacgcat ggagcagaac ggcgtggccc tggattcttc
    14281 catcctcaga gagctttccg cctccatcaa cacggaactc aagcgcctca ccgacctcat
    14341 ctatgactat gccggatatc agtttaacct caattccacc cagcaactgg ccaaactgct
    14401 ctttgaggaa aagggcctgc ccaccaaaaa gaagacaaaa agcggctttt ccaccgacag
    14461 cgccgtgctg gaagagctgg ccgacgatca cgagatcgcc cgcctcctga tccaataccg
    14521 catgctcagc aagctggaat ccacctatgt gaacaccctc cccaagctga tcaatccagc
    14581 cactcagagg gtgcactcct ctttcaatca gacggttgct tccaccggaa ggctctcttc
    14641 ctccaatccc aacctgcaaa acattcccat ccgcaccgag ctgggacgcc aggtccgcaa
    14701 agcctttgtg gcttccgctc ccaatcgtgt gatcctggcc gcggattatt ctcagatcga
    14761 gctgcggctg ctggccctct tttccaaaga cgaggtgctg ctgcaggcct tccgggacgg
    14821 cctcgatatc catcgccaga cggccgccta tattcacaac aagcctctgg aagaggtgag
    14881 ctctgatgag cggcgcggcg ccaagaccat caactttggc ctcctctacg gtatgggaca
    14941 aaagaagctc tcgcgcgagc tgggcatcag cctggcggac gccaaagaga tgatccgcaa
    15001 ctactttgag cgctttccca ccatccggga ttacatccgc tcctctatcg acgcggcccg
    15061 tcaaaaccgc tattgcgaga ccctctttgg ccgcaaactc tatctgccca atatcaacag
    15121 caataaccag ggcctcaaaa gcgaggccga acgcgtgagc gtcaatatgc ccatccaggg
    15181 cagcgcggca gacctcatca agatcgccat gctggatatt cacgctcaga tcaaagccga
    15241 agacgagatc cgcatggtcc tgcaggtgca cgacgaactg gtctttgacg tcctggcaga
    15301 ccgcgtggat gaggcctccc acatcatccg ctccgccatg gaaaacgctc tgccgcagca
    15361 gtatcgctcc atcatccacc tccagaccga tatcggagtg gggcgcgact ggtatgagtc
    15421 ccactgaggg agttggatag ttgtagggtt ttagagttgt agagttttgg agttgtgcta
    15481 atggagcggc aaaggttctc gtttgccaag gcgcgctcag cgcctgtgac aaagcctccc
    15541 agccccataa ggttttggga tatagcgcct tggagttcaa agcccct
//
LOCUS       DAOSFN010000048        15358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3160_length_15358_cov_9.386526, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000048 DAOSFN010000000
VERSION     DAOSFN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 15358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15358
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3160_length_15358_cov_9.386526"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLO35_04605"
     CDS             <1..361
                     /locus_tag="PLO35_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78980.1"
                     /translation="IMRGRLIIFLLVAATAVFFNFWNARKVLNYTRSMDHIEKTVAAV
                     KNINTELEIEYDDLCSGQNISSLVSVELSNFIPEQQQGRIIYVHEPRSAETRDSYCIV
                     DLIATKAQAKDIQIIPD"
     gene            365..2539
                     /locus_tag="PLO35_04610"
     CDS             365..2539
                     /locus_tag="PLO35_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOH78981.1"
                     /translation="MPSRYKFLVLVFALVATLWCVYLFGLQVLNPFDFSARITGRYIP
                     KKEILIPNRGPIYDAHGSLLVSSVRYYQVDIDRAAVDRWARYGQFSLDEGFARIAKAI
                     AEHSFMKAEDVMKRLKLGSRLTSIQIGNKFRSSELDRIIKAFDTQGLPGLSHSFASMK
                     RIYSKDIVAARLMGSVTAESDGFDPLTLSKSLYKLAGICGVEASHDHLLAGEYGWREY
                     VEDANHQRVPYPNLHEKRPVNGLGIRLTIDSNVQEVVENALREGLEKYSAQNAGAVVM
                     DPKTGKILAMAGVSIDDRAVDPNLVRVRANIPSTFMYEPGSTMKPLTMLAALDYKLVR
                     PDERIPCGVYQVGRRTISDTHFYGPLQPIDIISKSSNVGVARIAERIGPSRLYEKFIS
                     YGFGQKTPLNLYGETSGQFAKLANWDGYTLHSISFGQGLSVTALQQAIALSAIANGGK
                     MMKPMLVDAYLDERGRVLEQFEPAVLRQVSSQASADTMLTYLQAVVDRGTGRHIKMDY
                     ISLGGKTGTAQKNVEGTRGYSSGKYTSVFTGVFPIEDPRMVIVVFYDEPAPGFHYGST
                     SAAPTFRKIVEDILFMPNCDILAFNERLMQASLTMPDLKGKHLREAEAILNRYGFLYK
                     IEGADSSAVVVDQFPKANVAVERGHPITIKVGKSASTPGTAVPVGQMPDLKGMTLRKA
                     LLTASAHRVRLNIRGSGIVQSQSVLPGSKLYPGATCIVEASL"
     gene            2536..5391
                     /locus_tag="PLO35_04615"
     CDS             2536..5391
                     /locus_tag="PLO35_04615"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOH78982.1"
                     /translation="MRLAEILSLFQAHCLRPQASLSGDSDPANHLDLDFNGTPHTDNR
                     LLNSGDIFICIKGFSTDGHQFIPDAIARGASLIVCEQECQPGLPSIRVTDTRKAAALL
                     ARLYFNCPSSSFRLVGVTGTNGKTSTSLIIFEALRRLGYKAGWIGTLGYMINDGHFPT
                     GHTTPDIMELNRIFASMRDAEVSYVIMEVSSHAIALDRIYGIDFDLCLFTNLSRDHLD
                     FHGSMEEYGRVKASFFEAVRGGKSVAVINIEDAFGAQLAESLKGGLGAVFTVGAGAAA
                     YQIQDLVCDRHGILCKLQTRAERLEISSALIGDFNARNLALAAAALHLLSFDSRDISS
                     ALATVNAVPGRMQSVPNQRGIGVFVDYAHTPDAIETVLKTCRKLPHNRVLCLMGAGGD
                     RDQGKRPLMLGAALRYADAVIVTDDNPRTENPDKIILDIISGSHPDLPWWIIRDRALA
                     IHSILTLAQPDDLVVICGKGHETYQEIAGVKHPFNDVEVASAWLNSAAFTDQEALTLP
                     IDELLLRVLNQDLTALKDRGYNPPRSYTYISTDTRSIQTGSVFYALKGERFDAHAFLG
                     KALDEPANFAIGELALPEHDRYLQVESSLGSMARLLAKYLAMFDIYKLALTGSTGKTS
                     TKEFLANICASSGPTLKTLANENNLIGLCQTIRRVRPDHRFGVFELGTNHFGEIATMS
                     DILNPDAALVINIGPSHLEAFGDEEGVYKEKSVLLRQPLQIRLFDGDDPRFAEFYDSG
                     KGVGFSEACQYRISDLKTQGDSMAFKLGGQEYRIPSTVPHMAFNAAFGIAFGLEKGFT
                     RQQIQSAVLRPLSLAHRLETEIAGDKLLIVDCYNANPVSMQRALEYWIALEAPRPHVA
                     ILGDMLELGDMAAGYHQMVGAIMAEMNYAMLITVGKLSIHYQPVEMAVKTHHFPDVEA
                     AIAGLGQFSLPKGAVILIKASHGIHLEKLLPMLRKGV"
     gene            5392..6495
                     /gene="mraY"
                     /locus_tag="PLO35_04620"
     CDS             5392..6495
                     /gene="mraY"
                     /locus_tag="PLO35_04620"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOH78983.1"
                     /translation="MLYHLLFPLAKYSIVFNVFRYVTFRSIGAFITSLILSLLVGPAF
                     IRMLKRMAAVETIDDDVPEKHRIKAGTPTMGGLIILTALMISSLLWNNLTNSSILMMY
                     LTTVWLGVLGFLDDYLKNFVKAKKGLVPKYKLWGQISVALLLTLAIYYSRELGSNVSA
                     IQIPFIKNMYLELGIFFIPFIVFFIVGISNAVNLTDGLDGLAAGVMIFAALGLGVMAY
                     LKGNFVAAGYLNLEFLSSAGELTVFIAAMIGTLIGFLWFNSYPAQVFMGDTGSLTLGG
                     ILAVISILLKEQIFFVIIGLIFVSETCSVIIQRSWFKYTKKRYGSGRRVFLCAPVHHH
                     FELKGLHETKIVIRFWIVAILLVAIGLSTIKLR"
     gene            6512..7867
                     /gene="murD"
                     /locus_tag="PLO35_04625"
     CDS             6512..7867
                     /gene="murD"
                     /locus_tag="PLO35_04625"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOH78984.1"
                     /translation="MFDPSIHYGILGMARSGIAAAYKIRELGGTAFLSEKLERAMIPA
                     AEDLEADFECEYGGHSERLLDCPVWIVSPGIPLTVPIIQKGRAAGVRMISEIEFGWQI
                     KHPDSKIIAVTGSNGKSTTASLIHHILQGMGLKSILGGNIGAAFCSYPITKAGLDYIV
                     LEISSFQLDTIDSFRPDVAVLLNITPDHLNRYESFDHYAASKFRVFENQMPTDTAVIC
                     VDSAPIVQRRDQIVARQLSYSMINTHPDVEAWMNGVFIQLGPKHRISIHDLGIKGPHN
                     HANAMAALLSVDALTHDMDAATEAARGFAPLSHRLEFVATIGGVSFYNDSKATNTDSV
                     ISALVSFDKPIRVIMGGSDKGEDFGVMTDMLRKWTQKVYITGETEAKMRQAWLGKLPL
                     VCIPDFEQCVRAAFAESLAGDIIVLSPACASFDKFQNFEHRGNVFKEIIQSIALENEK
                     K"
     gene            7854..8993
                     /locus_tag="PLO35_04630"
     CDS             7854..8993
                     /locus_tag="PLO35_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOH78985.1"
                     /translation="MKRSRSQHYIITYDKVVVTCYLLLCLAGLLIMLDLTSIQDAMGF
                     FYRHAIFLAVSLVTAIFILYFFNMEKLRGLNGAFIAIVIGLLIAVLIVGRTVKGGTRA
                     INLGFISIQPSFLARVALIFYYAHHLDKKHDELIKALPRDLWAHFYPLIVATGLVYGL
                     IILERHLSTLVISGMTLLGLLTYAGIRKRFVLAVSAIGVLAVIGIIMFGAQFRSSRIE
                     TFKTYFLLTRTAPSDSSQADDYQVKESITALTRGGWFGTGMSRGRAKHFYLPESRTDY
                     IFTIIGEEFGFIGAFLVLALHCVLFFRAFKIAHSQPNLYLRFLCAGLAMNIFFNALVN
                     TGVAMSILPATGNTLPFISYGGTALLVDSASVGVILNISARRREL"
     gene            8990..10312
                     /locus_tag="PLO35_04635"
     CDS             8990..10312
                     /locus_tag="PLO35_04635"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOH78986.1"
                     /translation="MTTKLRITRYALWGPGGLESDTTAHGNEDVSGSTTGNILNSQFS
                     ILNSLSIVFGCGGTGGHIIPALAIAREFSALGHRIVFIGNRDGMEASLVKREGYPFHP
                     IRVQKLSRGGDLSLLRFPFLLIGSSINCFTYYRKLRPNAVVCTGGFVSGPVAIAAILA
                     RIPLFFHESNSMPGLTTRLLARFTRITFVSWQAALKRLQNAPVRMSAIPLLPRDGSSI
                     QNVKTETNPAGDKGSSSEPVDPHTGLSSLGIDSSQPVILVSGGSQGSLMINRCIDAAL
                     DAMLARGWQLIWQTGQSGYQEYSSRHKGKTGVHIFAFSPQLPLFYQAAAAAVTRAGAM
                     TIAEQEEYRVPAILVPLPSAAANHQYYNAIGQEQKGVAICLEQKHLGPDSLIAALDRI
                     LQNRDAYLARLLAIPANTASRDISAAIISNLSAGPQVPSSSKEQHGAR"
     gene            10302..11720
                     /gene="murC"
                     /locus_tag="PLO35_04640"
     CDS             10302..11720
                     /gene="murC"
                     /locus_tag="PLO35_04640"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOH78987.1"
                     /translation="MLGKTRKIHFIGIGGIGMSGIAEFLHNQGLEISGSDQKKTEITR
                     HLEAIGIRIAEGHDPGHIQDADVVVKSSAVREDNPEIIAAKALKIPVIRRAEMLAEIT
                     RMSFSIGISGTHGKTTTTSMAGMVLEAAGLDPTIIVGGKVKNFGSNNVMGSGKYIVVE
                     ADEYDHSFLSLTPCIAGITNIDTDHLDCYRNLDDIKGAFVEYANKVPFFGSVICCLDD
                     PGVQAILPRINKKVLTYGFSRQADIQAQTVRMEDFRCSYELSYKGYNLGKVQMNVTGR
                     HNILNSLLAVGMGLELDIPFSAIQAGLAKYSGVYRRFELKGTAGGITVYDDYAHHPTE
                     IRATLEGFKDSTPRRIVVLFQPHLYSRTRDLYHEFGNSFFSCDQLILAPIYPAREEPI
                     PNINSKLIADVAIQSGHHGVICLDRNDEIVAKTMAILKPDDILVTMGAGNVWQYGEEI
                     LTTLQKSIDRNSKVERTSNLER"
     gene            11787..12542
                     /locus_tag="PLO35_04645"
     CDS             11787..12542
                     /locus_tag="PLO35_04645"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOH78988.1"
                     /translation="MRSTRKRRGNSRYYVYFIMVLLVTGLLGTGLYLLLMNVSWFNIR
                     QISHSGNLVVPDSLISSVLERYEGQNLLKVRGSELRSHFQKIARVQDIDIHKGLPHKL
                     NVKFHERQGVLWIRSAEGELRPIDKDGMVLETFGGVAKEDLPIVGTYLTLSQLKAGTK
                     LEKPYIRSVLALHNRISKESPEFLPFVSEYYFIDNTIHMVDSRYGTSIIPAQKNIAAQ
                     LKRYLFVQDNGNIARRSTVDLRFDKQVVVKEAN"
     gene            12542..13816
                     /gene="ftsA"
                     /locus_tag="PLO35_04650"
     CDS             12542..13816
                     /gene="ftsA"
                     /locus_tag="PLO35_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOH78989.1"
                     /translation="MKHGIITALDIGSSFVRSIIAKVGADKRLEILGIGEAESRGIEK
                     GIVTDIQALADTIKTSLTEAETVAGCDAVNIFVNITGEHIKTHLGDGRISIPTLGPNE
                     PGEISSEHVDQVINDAKNSIKIQKGFERSKILHGLSQSFVIDGQDDIRNPVSMNGFHL
                     IARVYTIFADVTPMRNLSKCIELAGYEMDPENFVLSHIALSHSALSADEKRLGCILID
                     IGGGTCDIALFNRGTLEKILVVPMAGVNITEDLAIGLKTTLNNAEYIKTQYGIALAEA
                     VDPVAEVEVEGISGRASSRKSQQLVSHVIQHRVEEMLTLCYNKLKNSYTPELVTAGIV
                     ISGGTANLQRVADVVESAFNLHVKIAHPDINGMNGMITRLEDPAFCTAVGLLQYACSL
                     DREFKQSGFGKASIKDLKIIEKIKKIYKDFTA"
     gene            13837..15060
                     /gene="ftsZ"
                     /locus_tag="PLO35_04655"
     CDS             13837..15060
                     /gene="ftsZ"
                     /locus_tag="PLO35_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOH78990.1"
                     /translation="MIEFDEKPAQIGTNIKIVGVGGAGGNAVNTMIEHGLLGVEFIAA
                     NTDMTDLVKSKATMKLQIGKKLTRGLGTGADPEKGYRSAEESKDEIKSHLEGADMVFI
                     AAGMGGGTGTGAAPMVAKIARELGILTLGIVTTPFTFEGKKRHTNAEEGIRNLSEFVD
                     TLILIPNSKLSEIYHDISLVDAFRKADNVLYEAAKAVSDIINVSGYINVDFADVKTVM
                     ANMGYALMGTGSASGEDRAVLAARAAINNPLLADVSLSGCQSLLINITAGYDFKMSEF
                     EAVNSVVVNETGRAANIISGVITDETISGDVFVTIIATGLQRNESTNTLSFPSIMPTV
                     QTAQPVQPKPVMTPAVQISKAEPPLEEEMDTIFTRLKLSQSENKDNHEARSAEETNKP
                     LNPRTDLPSFLKALD"
BASE COUNT         3591 a         4406 c         3969 g         3392 t
ORIGIN      
        1 aatcatgaga ggaagactga tcatctttct attggtggct gctacagccg tattcttcaa
       61 cttttggaat gccagaaaag tgctcaacta cacccgctcc atggaccata tcgagaagac
      121 tgtggccgcc gtgaaaaata tcaataccga gctcgaaatc gagtatgacg acctctgcag
      181 cggccaaaac atctccagcc tggtgagcgt ggagctcagc aattttatcc cggaacaaca
      241 gcaaggccgg atcatctatg tgcacgagcc cagatctgcc gaaaccaggg attcctattg
      301 cattgttgat ctgatcgcca ccaaagccca ggccaaagat atccagatta ttccggattg
      361 accgatgccc tctcgctata agtttctggt gctggtcttt gccctggtgg ccaccctctg
      421 gtgcgtgtat ctctttggcc tgcaagtgct gaatcccttt gatttttccg cccgcatcac
      481 aggccgttac atccccaaaa aagagatcct gatccccaac cgcggaccaa tctacgacgc
      541 tcacggcagt ctgttggtga gcagcgtgcg ttattatcag gtggatatcg accgcgccgc
      601 cgtggaccgc tgggcccgct atggccagtt cagcctggat gaggggtttg ccagaatcgc
      661 caaagccatc gcggagcaca gcttcatgaa ggctgaggat gtgatgaaac gcctcaaact
      721 gggatccagg ctcacatcca tccagatcgg caacaaattc cgctccagcg agttggacag
      781 gatcatcaaa gccttcgaca ctcaaggact gcccggcctc tctcacagct ttgcctccat
      841 gaaacggata tattccaaag acatcgtcgc cgccaggctg atgggctccg tcacggctga
      901 atctgacggc tttgatcccc tcactctgag caaatccctc tataaactgg ccggcatctg
      961 cggggtggaa gcctcccacg atcatctgct ggccggtgaa tatggctggc gcgaatatgt
     1021 ggaggacgcc aaccaccagc gcgtccccta tcccaatctg catgaaaaac ggcccgtcaa
     1081 cgggctcgga atccgcctca cgatcgattc caacgtccag gaagtggtgg aaaacgccct
     1141 tcgggaaggc ttggaaaaat acagcgctca aaatgccggc gccgtggtga tggaccccaa
     1201 aaccggcaaa atcctggcta tggcaggcgt ctccatcgac gacagagctg tggaccccaa
     1261 cctggtgaga gtgagggcaa acatcccctc cacctttatg tatgagcccg gctccaccat
     1321 gaaacccctc accatgctgg cggccttgga ttacaagctg gtgcgccccg atgaacgcat
     1381 cccctgcgga gtatatcagg tgggcagacg caccatcagc gatacccatt tctatgggcc
     1441 cctgcagccc atcgacatca tcagcaaatc cagcaacgtg ggcgtggcca ggatcgccga
     1501 acgcatcggg ccatccaggc tctatgaaaa attcatctcc tacggctttg gccagaagac
     1561 tcccctcaac ctctatggcg agaccagcgg tcaatttgcc aaactggcga actgggacgg
     1621 atataccctg cactccatct cctttggcca ggggctttcc gtcacagccc tgcaacaagc
     1681 catcgccctc agcgccatcg ctaacggcgg aaaaatgatg aaacccatgc tggtggacgc
     1741 ctacctggac gaacgcggca gagtgctgga gcaatttgag cctgccgtgc tgcgccaggt
     1801 gtccagccag gcctccgccg acaccatgct cacctatctg caagccgtgg tggatagagg
     1861 caccggccgt cacataaaaa tggactatat ttccctgggc gggaaaactg gcaccgccca
     1921 gaaaaacgtg gaaggcaccc ggggatattc cagcggcaaa tatacttccg tgttcacagg
     1981 cgtcttccct atcgaagatc cccgcatggt gatcgtggtg ttttatgatg aacccgctcc
     2041 cggatttcac tatggcagca ccagcgcggc gccaaccttc cgcaagatcg tggaagacat
     2101 cctctttatg cccaattgcg acatcctggc cttcaacgaa aggctgatgc aggcgtcact
     2161 tacgatgcca gacctcaagg gcaagcatct ccgcgaagca gaggcgatcc tcaatcgtta
     2221 tggcttcctc tataaaatag aaggggccga ttcctcggcg gtggtggtgg accaatttcc
     2281 caaagccaat gtggcggtgg aacgcggcca ccctatcacc ataaaagtgg gaaaaagtgc
     2341 cagtacccca ggtacggcgg tcccagtggg ccaaatgcca gacctgaagg gtatgacgct
     2401 gcggaaggct ttgctcaccg cttcggcgca tagggtcagg ctcaatatcc gcggatccgg
     2461 gattgtgcag tctcagagcg tgctgcctgg gtcaaagcta tacccaggag ccacttgcat
     2521 cgtggaggcc tcgctgtgag gctggcggaa atcctttctc tctttcaagc ccactgcttg
     2581 aggccccagg ccagcctttc cggggattcc gatccggcca atcacctgga tctcgatttc
     2641 aacggaaccc cccacacgga taaccgcctc ctgaacagcg gggatatctt tatctgtatc
     2701 aaaggattca gcaccgatgg gcatcagttt atccccgatg ccatcgcccg aggggccagc
     2761 cttatcgtct gcgaacagga atgccagccc gggctgccgt ccatccgggt caccgatacc
     2821 cgcaaagccg ccgccctgct ggcaaggctc tatttcaatt gccccagttc ctccttccgc
     2881 ctcgtcggcg taaccggcac gaatggcaag acatccacct ccctgatcat ctttgaggcc
     2941 ttgagacggc tgggctataa agccggctgg atcggaaccc tgggctatat gatcaacgac
     3001 gggcactttc cgacaggtca cactacaccg gatattatgg aactgaaccg catctttgcc
     3061 tctatgcggg atgcggaagt aagctacgtg atcatggaag tttcatccca cgccatcgcc
     3121 ctggacagga tctatggcat cgatttcgac ctttgtctgt tcaccaatct cagccgtgat
     3181 cacctggatt ttcacggcag tatggaagag tatggcaggg tgaaagccag cttctttgag
     3241 gctgtaagag gcggtaaatc tgtggccgtg atcaacatcg aagacgcttt cggggcccag
     3301 cttgcggaaa gcctgaaagg cgggctggga gctgtcttca cggtgggagc cggagcagcc
     3361 gcttaccaga ttcaagacct ggtgtgtgac cgccatggga tcctttgcaa gctgcagacc
     3421 agggccgaga ggcttgagat cagttcggcc ctgataggtg atttcaatgc caggaatctg
     3481 gccctggcag ccgcggcgct tcatctgctg agcttcgact cccgcgatat cagctctgcc
     3541 ctggcaacgg tgaacgccgt tccgggcagg atgcaaagcg ttccaaatca acgtgggatc
     3601 ggggtctttg tggattacgc ccacaccccg gacgccatcg agaccgtctt gaagacctgt
     3661 cgcaaactgc cccataaccg agtgttgtgc ctgatgggag caggcggtga ccgggatcag
     3721 ggcaaacgtc ctcttatgct tggcgccgcc ttgcgttatg ccgacgcggt tatcgtcacg
     3781 gacgacaacc cccgcaccga gaatcccgat aagattatcc tggatatcat cagcggttcc
     3841 catcctgacc tgccctggtg gatcatacgg gaccgggccc tggccattca cagcattctg
     3901 acactcgctc agcctgatga tctggtggtc atctgcggca aaggccacga gacctatcag
     3961 gagatcgcgg gcgtaaaaca cccctttaac gatgtggaag tggcttccgc ctggttgaac
     4021 agcgctgcgt ttaccgatca ggaagccctc acgctgccca tcgacgagct gctgctgaga
     4081 gtcctcaatc aagacctgac tgccttaaaa gaccgcggct acaatccccc ccgctcttat
     4141 acatatatct ccaccgatac ccggtctatc cagacgggct ctgtgttcta tgctctcaag
     4201 ggagagcgct ttgacgccca cgcgtttttg ggaaaggctt tggatgaacc cgccaacttt
     4261 gctataggtg agcttgccct tccagagcac gaccgctatc tgcaggtgga atcatcgctt
     4321 ggcagcatgg cgcgcctgct ggccaagtat ctggccatgt tcgatatcta caagcttgcc
     4381 ctcacaggca gcaccggtaa gaccagcacc aaagagttcc tggccaatat ctgcgcttcc
     4441 agtggcccca ccctcaagac cctggccaat gaaaacaatc tgatcggcct ctgtcagacc
     4501 atccgtcgcg tcaggccaga tcaccgcttt ggcgtctttg aacttggcac aaaccacttt
     4561 ggcgaaattg ccactatgtc tgacatcctc aatccggacg cggccctggt gatcaatatc
     4621 ggtccctctc acctggaagc gtttggcgac gaggaaggcg tatataagga aaagagcgtg
     4681 ctgctcagac aacccctcca gatcaggctt ttcgatggag acgatccccg cttcgccgag
     4741 ttttatgaca gcggcaaagg ggtgggcttc agcgaagcct gtcaataccg gatcagcgat
     4801 ctgaagaccc aaggcgatag tatggctttt aagctcggcg gccaggaata ccggattcct
     4861 tctacggttc cccacatggc tttcaatgca gccttcggga tcgccttcgg gctggagaaa
     4921 ggctttaccc ggcagcaaat ccagtcagcc gtgttgcgcc ctctatccct ggctcacagg
     4981 ctcgagaccg aaatcgccgg cgacaagctt ctgatcgtag actgctacaa tgccaacccc
     5041 gtttccatgc aacgcgctct ggagtattgg atcgcgctcg aagcgccgcg cccccacgtc
     5101 gccatattgg gagacatgct cgaactgggg gatatggcag ccggttatca tcagatggtg
     5161 ggggccataa tggcagaaat gaactatgca atgctgatca cggtcggcaa gctgagcatc
     5221 cactatcagc ctgtagagat ggctgtcaaa acccaccatt ttccggacgt ggaggctgcc
     5281 attgcaggct tgggccagtt tagcctgcca aagggggcgg taatcctgat caaggcctcg
     5341 cacggcattc acctggaaaa gcttctgccc atgctcagaa agggagtctg aatgctttat
     5401 catctgctct tccccctggc aaagtacagc atcgtgttca acgtcttccg ctatgtgacc
     5461 tttcgctcca tcggcgcgtt cataacctcg ctgatcctct cgcttctggt gggaccggcc
     5521 tttatccgca tgctcaaacg catggccgcg gtggagacca tcgatgatga cgttcccgaa
     5581 aagcatcgta tcaaagctgg aacccccact atgggtggct tgatcatcct caccgctttg
     5641 atgatctcat ccctgctctg gaacaacctc accaattcct ccatcctgat gatgtacctt
     5701 accaccgtgt ggctgggagt gctgggcttc ctggacgact atctcaaaaa ctttgtgaag
     5761 gccaagaaag gcctggtgcc caagtataag ctgtggggac agatcagcgt ggccttgctg
     5821 ctcaccctgg ccatctacta cagccgcgag ctgggctcca acgtctcggc catccagatc
     5881 ccgtttatca aaaacatgta tctggagctg ggcatctttt tcattccctt catcgttttc
     5941 ttcatcgtcg ggatctccaa tgccgtcaat ctgaccgatg gcctcgacgg tctggcagcc
     6001 ggagtgatga tcttcgccgc gctgggtttg ggagtgatgg catatctgaa gggcaatttc
     6061 gtggcggccg gctatctgaa tctggagttt ttatcctccg cgggcgagct caccgtcttc
     6121 atcgctgcca tgatcggcac cctgatcggc tttttatggt tcaattccta tcccgcccag
     6181 gttttcatgg gagatacggg atccctcact ttgggaggca tcctggcagt gatctccatt
     6241 ctgctgaaag agcagatctt tttcgtgata ataggcctga tttttgtatc agagacctgc
     6301 tctgtgatca tccagcgttc ctggtttaaa tacaccaaga aacgctatgg ttccgggagg
     6361 cgcgtcttcc tctgcgcccc tgttcatcac cactttgagc tgaaaggctt gcatgagacc
     6421 aagatcgtca tccgcttttg gatagtcgcc atcctgttgg tcgccattgg cctcagcacc
     6481 atcaaacttc gctaacaagg aaaatcatcc aatgtttgat ccctcaatcc actacggcat
     6541 attgggcatg gcccgcagcg gcatcgcggc ggcttataag atccgtgaac tgggaggtac
     6601 cgccttcctg agtgagaagc tggaacgcgc catgatccca gccgccgaag atctcgaagc
     6661 tgacttcgaa tgtgaatatg gtggccacag cgagcggctc ctggactgcc cggtctggat
     6721 tgtgagcccc ggcataccgc tcactgtccc gatcatccag aaaggccgtg ccgccggagt
     6781 caggatgatc agtgagatcg agtttggctg gcagatcaaa catccagaca gcaagatcat
     6841 cgccgttacc ggttccaacg gcaagagcac cactgccagc ctcatccacc atatcctcca
     6901 gggcatgggc ttgaaatcta tcctgggagg caatatcgga gcagcttttt gcagctatcc
     6961 catcaccaaa gcggggctgg attacatcgt cctggagatc tcatcatttc aattggacac
     7021 gatcgacagc ttccgtcccg acgtagcggt actgctcaac atcactcccg atcatctcaa
     7081 ccgctatgaa agcttcgatc attacgccgc ctccaagttt cgcgtttttg agaaccagat
     7141 gcccacggac actgccgtga tctgtgtgga ttccgctccc atcgttcaac gcagagatca
     7201 gatcgtcgcc aggcagctca gctattctat gatcaacacc cacccggacg tggaggcctg
     7261 gatgaatgga gttttcatcc agttgggccc caaacaccgc atttctatcc acgatctggg
     7321 catcaagggc cctcacaatc acgccaatgc tatggctgcc ctgctgagcg tggacgctct
     7381 cacccatgac atggacgccg ccacagaagc ggccagggga ttcgcacccc tttctcaccg
     7441 cctcgagttt gtagccacga tcggaggggt gagcttttac aacgattcca aagccaccaa
     7501 taccgattcc gtgatcagcg ccctggtttc ttttgacaag cccatcaggg tcatcatggg
     7561 aggcagcgac aaaggtgaag actttggtgt gatgacggat atgctgcgta aatggaccca
     7621 gaaagtctat atcaccggtg agacggaagc caaaatgcgg caagcctggc tggggaaatt
     7681 acccctggtc tgtatcccgg attttgagca gtgcgttcgc gccgcttttg ctgaatctct
     7741 ggcaggagat attattgtgc tgtcacccgc atgcgccagt tttgacaaat tccaaaactt
     7801 cgagcatcgc ggcaacgtct tcaaggaaat catccaaagc attgccctgg aaaatgaaaa
     7861 gaagtagaag ccagcactac atcatcactt acgacaaggt tgtggtgacc tgctatctgc
     7921 tgttgtgtct ggctggtctg ttgatcatgc tggacctcac ttccatccag gacgccatgg
     7981 gctttttcta ccggcacgcc attttcctgg cggtctccct ggtaacggct atttttatcc
     8041 tttatttctt taacatggaa aagctgcgcg gcttgaacgg agcgtttatc gcgattgtga
     8101 tcgggcttct gatcgcggtg ttgatcgtgg gccgcaccgt taaaggcggc acccgtgcta
     8161 tcaacttggg cttcatcagc atccagccca gctttttggc ccgcgtggcc ctgatctttt
     8221 attacgccca ccacctggac aagaagcacg atgaactcat caaagcccta cccagggacc
     8281 tatgggctca tttctatcct cttatcgtgg ccaccgggtt ggtctatgga ctgatcatcc
     8341 tggaacgcca tctcagcact ctggtgatca gcggcatgac ccttttgggt ctgttgacct
     8401 atgctggcat ccgcaagcgc tttgtattgg cggtttcggc tattggagtg ctcgccgtga
     8461 tcggcatcat catgtttggc gctcagttcc gctcctcccg catcgaaacc ttcaagacct
     8521 atttcctgct cacgcggacg gctccctccg attcttccca ggcggacgac tatcaggtga
     8581 aagaaagcat cacagcactc acccggggcg gctggttcgg gacgggcatg agcagaggcc
     8641 gcgccaagca tttttacctg cctgaatcca gaacagacta tatcttcacc atcatcggcg
     8701 aggaatttgg cttcatcgga gcatttttag tcctggccct ccactgtgtc ttgtttttca
     8761 gggctttcaa gatcgcccat tcccagccaa acctctattt gcgctttctc tgcgcggggc
     8821 tggcgatgaa catctttttc aacgccctgg tcaatacggg agtggcgatg tccatcctgc
     8881 cggccacggg taacactctt cccttcatca gttacggcgg aacggccctg ctggtggatt
     8941 ccgcctcggt gggggtgatc ctgaatatca gcgccaggcg gagggaacta tgaccacaaa
     9001 gttacgcatt acgcgttacg cgttatgggg gcccggcgga ttggaaagcg acactacggc
     9061 ccacggaaac gaggacgttt ccggctccac taccgggaac attctcaatt ctcaattctc
     9121 aattcttaat tctcttagca tcgtctttgg atgtggcggg acgggaggac acatcatccc
     9181 cgctctcgcg atcgccagag agttttccgc gctggggcac cggatagtgt ttatcggaaa
     9241 ccgggacggg atggaagcca gtctggtgaa gcgggaaggc tatcccttcc accccatccg
     9301 ggtgcaaaag ctcagccgcg gcggggatct ttccctcctc agatttcctt tcttgctgat
     9361 cggcagcagt atcaactgtt tcacttacta ccgtaaactg aggcccaacg ccgtggtttg
     9421 caccggtggc tttgtatccg gtccggtggc catagcagcc attctggcca gaatacctct
     9481 ctttttccac gagagcaaca gcatgccggg gctcacgacc agactgctgg ccagatttac
     9541 acgcattacc tttgtatcat ggcaagcggc tctgaaacgt ttgcagaacg cccccgtccg
     9601 gatgtccgcc attcctctgc ttccgcgtga tggatcgtct attcagaatg tgaagacaga
     9661 gactaatcca gcaggtgaca aaggtagctc ttccgaaccg gttgacccac ataccgggct
     9721 tagctctctg ggtattgaca gctcccaacc tgtaattctg gtatcgggag gttcgcaagg
     9781 ctctctgatg atcaaccgct gtatcgacgc cgctctggat gccatgctgg cgcggggctg
     9841 gcaactgatc tggcagacag gccagagcgg atatcaagag tattccagcc gccacaaagg
     9901 caaaaccggc gtccatatct ttgccttttc tccccaactg ccgctatttt atcaagcggc
     9961 cgcggctgca gtcaccaggg caggggccat gacgatcgcc gagcaggagg aataccgggt
    10021 gccggctatt ctggtaccgc tacccagcgc ggcggccaat catcaatatt acaacgctat
    10081 cgggcaagaa cagaagggcg tggccatttg cctggaacag aaacacctgg gcccagacag
    10141 cctgatcgcc gcgctggacc ggatcttgca gaatagagat gcctatctgg ccaggttgct
    10201 ggctatccca gccaacacgg cctcgcgcga tatcagcgcg gctatcatat caaacctctc
    10261 agcgggaccc caagtcccct catcctcaaa ggagcaacac ggtgctaggt aaaaccagaa
    10321 aaattcactt tatcggaatc ggcggcatcg gaatgagcgg tattgccgaa tttctgcaca
    10381 atcaagggct tgagatcagc gggtccgacc agaagaagac ggaaatcacc cgccacctgg
    10441 aagctatcgg cattcggatc gccgagggcc atgatccagg gcatatacag gacgcggacg
    10501 tggtggtaaa atcctccgcc gtaagggaag acaatcccga gatcatcgcc gccaaagccc
    10561 taaaaatccc cgtgatccgc agggcggaaa tgttggctga gattacccgc atgagcttta
    10621 gcatcgggat atctggcacc catggcaaaa ccaccaccac ttccatggcg ggcatggtgc
    10681 tggaagcggc tggactggac cccaccatca tcgtgggcgg caaggtgaaa aacttcggca
    10741 gcaacaacgt gatgggctct ggcaagtaca tcgtggtgga agcggacgaa tatgatcatt
    10801 cctttctttc tctcaccccc tgcatagccg ggatcaccaa tatcgataca gaccatttgg
    10861 attgctatcg caacctggac gatatcaaag gcgcttttgt ggaatacgct aacaaggtcc
    10921 cctttttcgg tagcgtgatc tgctgtctgg acgatccggg cgtccaggcc atcctgccca
    10981 ggatcaacaa gaaagtgctc acatacggat tttcacgtca ggcggatatt caggcccaga
    11041 ccgtccgcat ggaggatttt cgctgctcat acgagctgag ctataagggc tataatcttg
    11101 gcaaggtcca gatgaacgtg accgggcgtc acaacatcct caattccctc ctggcagtgg
    11161 ggatgggcct ggagctcgat attcccttta gtgcaatcca ggctgggctg gcaaaataca
    11221 gtggcgtcta tcgccgcttt gagctcaaag gcacggccgg tgggatcact gtttacgacg
    11281 actatgctca ccaccccacc gagatcaggg caactctgga gggctttaaa gacagcactc
    11341 ccaggcgcat cgtggtcctg tttcaacccc atctttacag ccgtacccgc gatctttatc
    11401 acgaatttgg taattccttt ttctcttgtg atcagttgat tttggcgccc atctatcccg
    11461 cccgggaaga gcctatccca aacatcaaca gcaagttgat cgcggacgtg gccattcaaa
    11521 gcggtcacca tggcgtgatc tgcctcgaca ggaatgacga aatagtggcg aaaaccatgg
    11581 ctatcttgaa gcctgacgat atcttagtta ctatgggcgc cggtaatgtc tggcagtatg
    11641 gagaagaaat tcttactact ctgcaaaaaa gtattgacag aaatagtaag gttgaaagaa
    11701 ctagtaactt agagcgttga gaccattatg cgcgattcca gctgcagatt cctaagcact
    11761 ataaaaatca ataaggtatt aaattcatga gatcaacccg caaacgccgc ggtaatagca
    11821 gatactacgt ctatttcatt atggtcttgt tagttacagg gcttttaggg acgggtcttt
    11881 atctgctctt gatgaacgta tcatggttta atatccgtca aatttcacac agcggaaacc
    11941 tggtagttcc ggacagtttg atcagctccg tcctggaaag atacgagggc caaaacctcc
    12001 tgaaagtgag gggttcggag ctcaggtccc actttcaaaa gattgcccgt gtccaggata
    12061 tcgacatcca caaaggcctc cctcacaagc tcaacgtcaa gtttcacgag cgtcaggggg
    12121 tgctgtggat ccgcagcgcg gaaggtgagc tcagaccaat cgacaaagac ggcatggtgc
    12181 tggaaacctt tggcggcgta gccaaagagg atcttccgat cgtgggcacc tacctcacgc
    12241 tatcacagtt aaaggcaggc accaagctgg aaaagccata tatccgcagc gtgttagctc
    12301 tccacaaccg gatcagcaag gaatctccgg aatttctccc cttcgtatca gaatattatt
    12361 ttatagacaa caccatccac atggtcgatt cccgctatgg gaccagtatt atccctgccc
    12421 agaaaaacat cgcggcccag ttgaagcgtt atctcttcgt tcaggacaat ggcaatatcg
    12481 cccgccgctc cacggtggac ctcagatttg acaaacaagt ggtggtaaag gaagcaaact
    12541 aatgaagcac ggcatcatca ccgccctgga catcggctct tcctttgtgc gttccataat
    12601 tgccaaagtg ggagctgaca agcgtctgga aatactcggc atcggcgagg ctgaatcccg
    12661 cggcattgaa aagggcatcg tcactgatat ccaggccctt gctgacacca tcaagacttc
    12721 tttaaccgaa gcggagaccg tggccggctg cgacgccgtc aacatctttg tcaacatcac
    12781 cggagagcac atcaagactc atctgggaga cggaagaatc tccatcccca ccctgggtcc
    12841 caacgaaccc ggcgagatca gctctgaaca tgtggaccag gtgattaacg atgccaaaaa
    12901 cagcatcaag attcagaagg gattcgagcg ctccaagatt ttacacgggc tctctcaaag
    12961 cttcgtgatc gatgggcagg acgacatccg taaccccgtc agcatgaacg gcttccactt
    13021 gattgccaga gtctacacga tcttcgcgga tgtcacgccc atgcgcaatc tctccaaatg
    13081 catagagctg gcgggctatg agatggaccc tgaaaacttt gtgctcagcc acatcgcgct
    13141 cagccattca gctttgtcag ccgatgaaaa acgcctgggt tgcatcctga tcgatattgg
    13201 cggcggcact tgtgatatcg ctctttttaa tcgcggaacc ctggagaaga tcctggtggt
    13261 tcctatggcg ggcgttaata tcacagaaga ccttgccatt ggtctcaaaa ccactttgaa
    13321 caatgctgag tatatcaaga cgcaatacgg catcgcgctg gccgaagctg tagatccggt
    13381 ggccgaagtg gaagtggaag ggatcagtgg ccgcgcctcc agccgcaaga gccaacaact
    13441 tgtgagccat gtgatccagc atcgcgtgga agagatgctg accctgtgct acaacaagct
    13501 caaaaactcc tacacgccgg aacttgtcac cgccgggatc gtgatctcgg gtggaacagc
    13561 caatctgcaa agagtggccg acgtcgtgga aagcgctttc aacctccacg tcaagatcgc
    13621 ccatcccgac atcaacggca tgaacggtat gatcaccaga ttggaagatc ccgctttctg
    13681 caccgcggtg gggctgttgc aatacgcttg ctctctggac cgcgaattca agcaaagcgg
    13741 ctttggaaaa gcatccatca aagacctaaa gattatcgag aaaatcaaaa aaatatacaa
    13801 ggacttcacg gcctaagggg gtccaggggg aacaaaatga tagaattcga cgaaaaacct
    13861 gcgcaaattg gcaccaacat caagatcgtg ggggttggcg gagccggtgg caacgcggtc
    13921 aacaccatga tcgaacacgg ccttttgggg gtggaattca ttgccgccaa tacggatatg
    13981 accgacctgg tcaaatccaa ggccaccatg aaactgcaga tcggcaagaa acttacccgc
    14041 ggtttgggca ccggagccga tcctgaaaag ggataccgca gcgcggagga atccaaagac
    14101 gagatcaagt ctcatctgga aggcgcggac atggtgttca tcgccgccgg catgggtggc
    14161 gggaccggaa caggcgcggc acccatggta gccaagatcg cgcgcgagct tggtatcctc
    14221 accttgggaa tcgtgactac acctttcact tttgaaggca aaaaacgcca caccaacgca
    14281 gaagaaggca tccgcaacct cagcgagttt gtggataccc tcatcctcat tcccaattcc
    14341 aaactcagcg agatttacca cgacatcagc ctggtggacg ctttccgcaa agctgacaac
    14401 gtgctctatg aagccgccaa ggccgtctcg gacatcatca acgtgagcgg ttatatcaat
    14461 gtggactttg ccgatgttaa gaccgtgatg gcaaacatgg gctacgccct gatggggacg
    14521 ggcagcgcat ccggagaaga cagggccgta ttggcagccc gggccgccat caacaatccc
    14581 ttgctggcag atgtcagcct cagcggttgc cagtcactgc tgatcaacat cactgctggc
    14641 tacgacttca agatgagcga attcgaagcc gtcaacagcg tggtcgtcaa cgaaaccgga
    14701 cgcgccgcca acatcatatc tggcgtgatt acagatgaga ccatctccgg agacgtgttt
    14761 gtgaccatca tcgccaccgg actgcaacgc aacgaaagca ccaacaccct cagtttcccc
    14821 tccatcatgc ccaccgtcca gaccgcgcaa ccggttcagc ccaaacctgt tatgactcca
    14881 gccgtccaga tttccaaagc tgagccccct ctcgaagaag aaatggacac catttttacc
    14941 agactaaaac taagtcagtc agagaacaaa gacaatcatg aagcaaggtc cgccgaggaa
    15001 accaacaaac cactcaatcc ccgcaccgac cttccttcct tcctgaaggc cctcgactga
    15061 tccaataaag cttccctgct taggtctcac aacggacagc cctgtccgcg caatcgccgg
    15121 ctacccccca gccggcggtt ttttatgttg tagagttgta aggttttagg gttgtaaagt
    15181 tttgtggttt tggagttgtg ggattgtggg gttttagagt tttgggtttt gaggttttca
    15241 agttgtcgag atttcgaatt atcaggttga gaccgttgca caggatctgc cgaaaccaga
    15301 gactaacata gccccgttgg agttcaagcg gccacgataa gagtatatat ggcagcga
//
LOCUS       DAOSFN010000049        15098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3228_length_15098_cov_9.031842, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000049 DAOSFN010000000
VERSION     DAOSFN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 15098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15098
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3228_length_15098_cov_9.031842"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLO35_04660"
     CDS             complement(<1..737)
                     /locus_tag="PLO35_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HOH78991.1"
                     /translation="MGQLLKLAKASADQADIYWEEVSYDHIEFTDAKLEKADSSLSYA
                     LALRVIKDGKCGLAHTRNLLDAKELVKQALTSAKDGQAVGFQLPASWQAPDSDCLDTR
                     IETIDKALLIAQGKELLAYLKARSDAQVNLSFNYGSSRIGLQNSYDLDLAWKGSTYSV
                     WTQMVFPGSGAGLMELAIGRGPVELDKIQLDRMLELFKISSTQVVPPTCKMPVIFTPL
                     TVGALFSRFYSAISAVNIHNGISPLCN"
     gene            complement(737..2101)
                     /locus_tag="PLO35_04665"
     CDS             complement(737..2101)
                     /locus_tag="PLO35_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH78992.1"
                     /translation="MIDKLNKLIEPLDVDYCDIRYETRRVDRVVMNKGEVRSIGSNTG
                     DGYVLRVLKDGALATVCFTKPEQAELAIRRSLENAKVLAANMAEPKRMAPAPRVVDTV
                     KARLIEDPRAIDLAEKLAITRHYSDLVMAQPAIANAELEYYDVYRDKYYVNSEGTQIN
                     EELVTTKLGGSIIAQEGPLTQTVRMAFGGSNGFQKLRNRDAEALEKCKIAQELLSAEP
                     VQAGSYRMVLNPGMAGVFTHEAFGHFSEADIVRDLPALREKMKIGTQLGSDILNIIDD
                     ATMDGQLGYYKYDDEGVAVRRVNLMSAGVLTGRLHDRFTAGEMDEPLSGHAIAEDFQY
                     APIVRMGNIFIKPDRYSLEELLAAMDEGLYIGDPMGGQTSGENFTFGANYGYKVKGGK
                     IVGMIRDINIVGNLYQTLANVLMIGSDFVLKESGGCGKGQTNIRSCYGGPHVYFKMLT
                     VGGK"
     gene            complement(2138..2629)
                     /locus_tag="PLO35_04670"
     CDS             complement(2138..2629)
                     /locus_tag="PLO35_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOH78993.1"
                     /translation="MNQQHEDTLNRIASLDAGINNPARLMIVFLLSRTEALDYNSVLA
                     RTRLTSGNLTTHLIKLEELGYIKLNKSFINRKPHTLIQITTSGREAYLNWGESVSHAL
                     PEEIKARQRPPLYWPLGLLKRYPLSLREWSFALPDNRGFASLPDGFLSFDLPPIPGTE
                     MIL"
     gene            complement(2985..8987)
                     /locus_tag="PLO35_04675"
     CDS             complement(2985..8987)
                     /locus_tag="PLO35_04675"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOH78994.1"
                     /translation="MKTKLVILAILLSVCALSAEQMIVGTVSGNDQGHPLSSYRGYVR
                     SAAIYNPSELGAINLQIDELAWYCGVTTTAAVPVRIYLKATTATQLQACTWSSITFGA
                     TQVYSGTMNGTTANSWQVFSLDSVFRIDAGLSLLVLVETIYRVSGSGTAGGGYFGPKV
                     SYTWYGAGPVYYHQVWSQNHTPLIGDGTLERGRPVIRINYTPFTTSGLPCPPMLSYPL
                     DGELRVHPTSILSWSSGGGWPTGYKLYFGQDPVPSAHQDIGTAQLFDPDSLAFSHTYY
                     WQVIPYNALGDALDCPVWSFTVRPDSVISSFPHLETFDDPWVGYIAAPFDWKMVNNDS
                     DDNTWKKGNTNLSPTPSPPYAAVAQSARNDWLITPPINLSGVNAKIEWWERCSSSSAN
                     SYRVMLSTTDNSIGAFTVELGSFTCSLNSWARREISLFPYNGETIYLAFHNLQAPNIA
                     SNYGIDNFKIIQIYDCDLSAVSLATDPQPFMGSPSLGRVRVQNPGLETQSGYLVQIVS
                     STNQILASQPGTEVLPGEYLDLNINFVAPSAGVDSVFARVVLVGDSYQPNDQSPWSVY
                     QARLPEPYQQVIGSGNVDSTIPLGFSYRASLFQTIYSHQAIGNWGSIPEITLYTSSSV
                     ALYDKQIKIWMQSTDLGNLSEGWVPVRDQILVFNGLIDIQTGISTVTIPLDTPFNYCR
                     GSLLLTLERCYDSGALQTGVNIMSQSDLANRSRYARYISTWDPNNPQYQNLSPIYPKL
                     GLQINPFSQGPEPHVYESAHDFGVLAPGSNNSQTFRIYNIGASDLTITGASFNQGTDL
                     SIDELPVPINLIFGQSFDLTVRYRPTEPTTLAATLSIQTSPGNQEVSVQLQALVEDKL
                     NLGVPTLTQNLPFSLNYGNAVYETIYQAQSLLNFDGVIHGIRLYHSPAVVDINVPVTI
                     WLGPTDLNDLSTGWINAGQLNLVYDGPLLITQDASYFDILFSHPYQHNMQNLALLVHT
                     GLHPSISNPPGFMAMNGNSNQTRHNSSNTTVFDPHSLSAGSLSNVWPQTSFLVSSTLG
                     EPLLRISPANPTFTTVAGGWIQESLALSNYGGRGLRVTAVDLSGNPDLSIDPTLTLPI
                     DLQAGESAELLFTFAPADTLDLPGQITLSYIKDPLTQPVDASLQVNMTCNALTDIVIG
                     SGTTQARRPIDFSQASSLYQAIYYPSELEHFQGFVNGLSFYNNFASAFMDQPTQIWMG
                     ITSRVDMTEGPIPSSQMTLVFDGMVDYPAGQNTVTIEFDQPYIYLQDQNLVISVFRPM
                     SAEFNNNACNFLCQNGVGARAWIIDSSDEFPLPESPQGGDPIAAFPKTRLSVLPLQGP
                     AQFHINSTDAAFGELALGLSKVKDYLIQNTGTMPFTLNSISLAGSPVFNLSHQQDLPL
                     LLEPGDGFSFSLGFSAIQPGSHEALLTINDDLSRNPGARNRTQRTIPVSGTALSAIQV
                     GFNSEVSRMPVAMNYRNSIYENIFQQTELTGLTGYITGLRWSYRFMDNLADKPIRIWL
                     GNTPTANLAAGWIPATQLTLVYDGSQSYQSGIHDLDIGFDTPFLYQGGNLAMMVQRPM
                     DTQYFSAFDQFFCSTVGNNRSRKIQHDSTIYDPNSPPTTGSLLSGQFPNTIFFFDSAL
                     SVSVSGRILAQHSLNPIAGASIELSGVDTYVAVSATDGVFQFPQIPVNSAYQYTVSAP
                     GYQSISGNVSIAFSDNQMGDLLLLETAYPPANVSAELDVFSRHATITWQATDEGQAVR
                     VMPSRSLAVDSRSRVRSEHRILTGFHLYRLAAGQETVPDTWIHISELDVNTLGFVDSL
                     WIEEPLGEYRWAVQAIYTGANLSPVVFSNILSNHVQMGQVAGMVQDDESQPLIGAQVS
                     AGDYAAATDGDGNYLLELPAGVYTITATAAGYASASHSDVMVLPDQPLTLDFTLDAVS
                     NPGDEVPALVTALQSNWPNPFKHQTNIRFSLQIGSPVSIRIYDIRGRLLRSVINETRQ
                     AGIHNVVLDASDDQGRRLPAGVYLCRFQARGYEHTIKLLLLH"
     gene            9429..11051
                     /locus_tag="PLO35_04680"
     CDS             9429..11051
                     /locus_tag="PLO35_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78995.1"
                     /translation="MADTTTRLVYGLTDAILSRFKQQLFISAVVQWRNALLESEVSYF
                     FPNLAQTLQSVDPQSFQTSYRLWQQAIEQDLIESPLGILNYTVQRLESSKSIKIDELA
                     MLKELRVLLSDFYALRKVSNLHEFIVMATDGNSTHSPIFRLIGHTAKNMMDRSGELAI
                     ADLNTLFENSDKRDRYIEIVNQDIKDLRLFGDSLRITSTNYPAYQIIAQTVSDFFEKT
                     ADSGSDTITTEEGSTGKLDHSKLMSLYLNTCDKVMVQFYKLAATPNPPKWDIYYSTFI
                     KHRLDLVEIAKAAEAKNYKALLNRMIVTATKLATPDDPRPGSYGRFLNLIAAVAQSSE
                     DDKVSYRELMNSVLEPVGTYTYKRSSAFTLGINAYPGLGAGAEMLEADLDKAKGVGGL
                     ASPVGLELSWGRGLLGLRGIFFSALDLGAIATYRFSGETDASVNSPQVGWKQLVSPGV
                     YLLVQGKSHPLTLGFGAQLTPELRKVDTAGKQEDKNALRLGAFLSVDIPLLTLYVKHN
                     WFKGDNYESRAPRTWTADNPPADSGFSTASGN"
     gene            11098..11415
                     /locus_tag="PLO35_04685"
     CDS             11098..11415
                     /locus_tag="PLO35_04685"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78996.1"
                     /translation="MVEQALGIINLSGTARDWSKAVNLLEDAASLDDPRAQYYLGLNF
                     YNGEGVVKNYRKACFWFMLAAANDHAAASLSLEQCLRKLSEDDAELLSQQVHSWRQAH
                     ETR"
     gene            11402..12391
                     /locus_tag="PLO35_04690"
     CDS             11402..12391
                     /locus_tag="PLO35_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH78997.1"
                     /translation="MRRVKESWYRILLVLLSILSISLLAADPLMDYLERQAGAGDPEA
                     MFLMGRCFERGDRVEKNYETAVSWYQKSAGLGHSGAQTNLGSLTQMGRGIAQNDSAAV
                     KLFLLAAQQGNAFGAYNLALSLERGRGIEKDLTEAVKWFEVGASKRHLSSMIRLAGCL
                     ESGAGIEKDTDKAMSWYRLAAEAGSAATQMRLGSYYDNGIWVVSDQAEALKYYRMAAE
                     QGHADAQYNLGIKYARGEGVDEDQSQASYWILQAAQGGSPKAQELLAYRYLDGIGLPQ
                     SDTEAYFWLLIRSMDANAPMKSLRDKTEKKLSRALVNQVQERADRWVEKLTGP"
     gene            complement(12378..>15098)
                     /locus_tag="PLO35_04695"
     CDS             complement(12378..>15098)
                     /locus_tag="PLO35_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH78998.1"
                     /translation="ISIDSLQTVLLTSSADKSARLWDLSDGRLRQVLRPPIGLERIGR
                     LHAGALSPDKRFAAVGGHDEEGGLYVFALDTMDIVFRKGALADRINQIEFSPDGRYLA
                     VGLITREVLIMDSRDWSLVAVLEFGAVRVGGLSFAGDGRLAVTCQDGRVRIYSSDFSS
                     FSQYRFSGDSKPYMVRFSPDGSKLVVSFNESFRLAMLDAKDLKPLYEPDTSDMVGEIY
                     EYLIPSFSADSQTLYAGGSCQAASLPGSRYTIRAWKDQGRGEYADISVSNNSITDLAP
                     RHKGGIFFCGMAPDWGILNADNQIEMYHAPEINDFNLWDKLHLRVNASGSEVGFTSWG
                     SQPREFRLADRIVYEWPSAHPAFTDSSDVIKITNWDQSFRPRLNGRGMSFLRDEEQNL
                     CVDVVDGGQRLLFGTIWNLYCLDAQGAYLWEVSAPAMVMAVKASGDGRLAVAALSDGT
                     IRWYRMEDGQLLMTLFSSRDQARWILYTPEGYYDCSPGSETMIGWQLNQGYEQPSIFY
                     PVEKFREEFYRPDLMKTLLVTLDSRQSLMLASEETGEEISSKDIKSALPPRLEILSPI
                     DGSSYNSPEVTFTYRLTDPANHKVKAIKVKVDNFSVLNIKDPLLTNGVGSFTLKLQPR
                     NTRIKISAEGGRGIDVKELSLKWAGDESKIVSLPKPRLFVLAVGVAAYQNSIKRLNFP
                     PKDASDFANALQLMAKPMYDSLAVRIITDSRATLRGVIEGFDWIASLCGPSDVAMIFL
                     SGHGTADKKGMYHFVTVDADADSIESTCLSYSVLEEKLKAIAGKVVLFTDTCHAGGIY
                     DDPRFRLPDTTGLVNRVSSDEVSVVVFASSTASQLSQEKVAWNNGAFTKVLVEGLLGK
                     ADLLGKKEVTFKGLDYYIAAQVKELTQNSQSPVTMPARIPDFVMIKLRDQ"
BASE COUNT         3480 a         3957 c         4203 g         3458 t
ORIGIN      
        1 ctgttacaga gaggagagat gccgttgtgg atattgacgg ctgagattgc ggagtagaat
       61 cggctgaaaa gcgcccccac cgtgagaggt gtaaaaatga cgggcatttt gcatgtgggc
      121 ggcacgacct gggtggagga aatcttgaaa agctccaaca tcctatccag ctgtatctta
      181 tccagttcta ctggtcctct cccgatcgcc aattccatca gtccggcccc gctgcctgga
      241 aacaccatct gggtccagac ggaataggta gatcctttcc aggcaaggtc caggtcgtag
      301 ctattctgca agccaatcct ggaactgccg tagttgaaac tgaggttgac ctgagcatcg
      361 ctgcgggcct tcagatacgc gagcagttct ttgccctgag cgataagtaa ggccttatca
      421 atagtctcga tccgggtgtc cagacagtcg gaatcaggag cttgccagga tgccggaagc
      481 tggaatccca cggcctgtcc atctttggcg ctggtcaaag cctgttttac caattccttg
      541 gcatccagca ggttgcgggt gtgggccagg ccacatttgc catctttgat cactctcagc
      601 gcgagggcat aggaaagcga gctatcggcc ttttccagct tggcgtcggt gaattcgatg
      661 tgatcgtaac tcacttcctc ccaatagata tctgcctgat cggcagaggc tttcgccagt
      721 ttgagcagct gtcccattat tttcctccca ctgtgagcat tttaaagtaa acatgcggtc
      781 cgccatagca ggaacggatg ttggtctggc ctttgccaca gcctcccgat tccttgagca
      841 caaagtcgga accaatcatc aggacgttgg ccagggtctg atagagattg ccgacgatat
      901 tgatgtcgcg gatcatacct actatcttgc cgcccttcac tttataaccg taattggccc
      961 caaaggtgaa gttctctccg gaggtctggc cacccatcgg atcaccaatg tagagccctt
     1021 catccatggc cgccagcaat tcttccaggc tgtagcggtc cggtttgatg aagatattgc
     1081 ccatacgcac gatgggcgcg tattgaaaat cctcggcgat ggcgtgaccg ctgagcggct
     1141 cgtccatttc cccggctgtg aaacgatcgt gcaacctgcc tgtcaacaca ccggcgctca
     1201 tcagattcac tctgcgcacc gcgacgcctt cgtcgtcgta tttgtaatag cccagttggc
     1261 cgtccatggt ggcgtcgtcg atgatgttga ggatgtctga gcccagttgg gtgccgatct
     1321 tcattttttc acgcagagct ggaaggtcgc gcacgatatc cgcctcggaa aaatgcccga
     1381 aggcctcatg ggtgaaaacc cctgccatgc ctggatttaa caccatcctg tagctacccg
     1441 cctggacggg ttccgcgctc agcaattcct gggctatttt gcacttctcc agagcttcgg
     1501 cgtcccggtt tctcagcttc tgaaatccgt tgctgccgcc aaaggccatt ctcaccgtct
     1561 gggtgagcgg gccctcctga gcgatgatcg agccgcccag cttggtggta accagttctt
     1621 cattgatctg ggtgccctcg ctgttcacgt aatacttgtc ccgatagacg tcataatatt
     1681 ccagttcggc gttggcaatc gcgggctggg ccatgacgag atctgaatag tgacgggtga
     1741 tggccagttt ttcggccaga tcgatggcgc ggggatcctc gatcagcctg gctttgactg
     1801 tgtccacgac cctcggagcg ggagccatgc gtttgggctc tgccatgttg gcggccagga
     1861 ctttcgcgtt ttccagggac ctcctgatcg ccagctcagc ctgctccggc tttgtaaaac
     1921 acaccgtggc gagcgcgccg tccttcagga ctctcaacac ataaccgtct ccggtgttgg
     1981 aacctatgct gcgcacttcg cctttgttca ttacgacccg atcgactcgc ctggtctcgt
     2041 agcggatatc gcaataatcc acatcaagcg gttcgatcaa tttgttgagc ttgtctatca
     2101 tatcatctcc ttagcggcca tagccgcgtg atttggatca aagaatcatt tccgtgcctg
     2161 gtatgggtgg cagatcgaaa ctgagaaaac catccggcag ggacgcgaaa ccacggttgt
     2221 ccggaagcgc gaaactccat tcccgcaaag acaaaggata acgcttgagc agtcccaaag
     2281 gccagtagag gggaggacgc tgcctggctt tgatctcttc tggcagagcg tggctaacgc
     2341 tttctcccca attgagataa gcctccctgc cgctggtcgt gatctgaatg agcgtgtgag
     2401 gcttgcggtt gatgaagctt ttgtttaact ttatatagcc aagctcttcc agtttgataa
     2461 gatgcgtggt gaggtttccc gacgtgaggc gggttctggc cagcacgctg ttgtagtcga
     2521 gggcttcagt gcgtgaaagc aggaaaacga tcatcagtct ggcgggattg ttgatcccgg
     2581 cgtccaggct ggcgatgcgg ttgagcgtat cttcatgctg ttggttcata taagactccc
     2641 ggcggcgctt atgccgcaca agttaccttg tatttcaaag tagctttcct gtcaagataa
     2701 atctgcggcc ccacacaaga tttttcacac ccgctcccct aagcctcctt tacaccacct
     2761 atgcctcacc tttacatctc ctttgcctcg gcataggaga ggcaggtgag gagtaaatgt
     2821 agggaaagga tggcagagca ggctgttaga ggatttatcc ggctccggga atgccgaaat
     2881 agaacttgcc cgtcaggtag aggcaaactg cagctatctc acgacttcag atcgcgcctc
     2941 tcatcttctc atatctcatt gtgaaatatg acagccagag cagctcagtg caacagcagc
     3001 agcttgatcg tatgctcgta tccccgggcc tggaagcggc agagataaac ccctgcggga
     3061 agacgtcttc cctgatcgtc actggcatcg aggaccacat tgtggattcc tgcctggcgg
     3121 gtttcattga ttacagacct cagcagccgg cccctgatgt catagattct gatgctgacc
     3181 ggtgatccaa tttggagtga aaaacggata ttggtctgat gtttaaaggg attgggccaa
     3241 ttggactgca gggctgtgac caaagccggg acctcatccc ctgggttcga cacggcatca
     3301 agagtaaaat caagggtaag gggctgatcc ggcaacacca tgacatcaga gtgactcgcg
     3361 ctggcataac ccgctgccgt ggcagtgatt gtgtaaactc ctgccggcag ttccagcaga
     3421 taatttccgt ccccgtcagt ggcggcagca taatccccgg cgctcacctg agctccgata
     3481 agcggttgtg attcatcatc ttgcaccata ccagccacct gccccatctg cacatgattg
     3541 gagaggatgt tgctgaagac gaccggcgaa agattggcac ctgtatagat ggcctggaca
     3601 gcccagcggt attcacccaa gggctcttca atccagagcg aatcgacgaa gccgagggtg
     3661 tttacatcga gttccgagat atgaatccag gtgtctggca cagtttcctg acccgccgcc
     3721 agacgataga gatgaaagcc cgtcagaatc ctatgttctg atcgcacccg ggaacgggaa
     3781 tcgactgcca gagagcggct tggcatcact cgaacagcct ggccttcgtc agtagcctgc
     3841 caggtgatgg tggcatggcg gctaaacaca tccagttccg cggaaacatt agcaggggga
     3901 tatgccgttt ccaatagtag caagtctccc atctgattat ccgagaacgc tatagagaca
     3961 ttaccgctga tgctttgata gccgggagcg ctcaccgtgt attgataggc tgagttcacc
     4021 ggaatctgag gaaactgaaa gactccatcc gtcgcgctga cagccacata ggtatccaca
     4081 ccggagagct caatgctagc ccccgcgatg ggattcagac tgtgctgggc caatattctg
     4141 ccgcttactg acacgctcaa ggcactgtca aagaagaaga tagtattggg aaactggcct
     4201 gacagcagcg atcccgtggt gggtggcgag ttgggatcat agatggtgga atcgtgttga
     4261 attttacggg agcggttgtt gcccacggtg ctacagaaaa actggtcaaa cgcggaaaaa
     4321 tactgggtgt ccataggcct ctgcaccatc atcgccagat tcccaccctg atacagaaag
     4381 ggagtgtcga agccgatgtc cagatcatgg ataccgctct gataactttg gctgccatca
     4441 tagacaagcg tcaactgggt ggcagggatc caacccgcgg ccagattggc ggttggggtg
     4501 tttcccaacc agatgcggat gggcttgtcg gccaggttgt ccataaatct gtagctccat
     4561 ctcaggccgg tgatgtatcc cgtcagacca gtcagttcag tctgttggaa gatgttctcg
     4621 tatatggagt tgcgatagtt catcgccacc ggcatgcggg agacttcact gttgaatccc
     4681 acctggattg cacttaacgc tgtcccagag acaggaatag tccgctgggt cctgtttctg
     4741 gcccccgggt ttctgctcag atcgtcatta atagtcagca gcgcttcatg gctgcctggc
     4801 tggatggcac tgaaccctag cgagaaggaa aagccgtccc ccggctccag caacagagga
     4861 aggtcctgct gatgagaaag gttgaaaacc ggagaaccgg ccaggctgat ggagttgagc
     4921 gtaaaaggca tagtgcctgt gttttggatg agataatcct tcactttgga cagccccaaa
     4981 gccaactctc caaaagcggc gtctgtgctg ttgatgtgaa actgggcggg accctgtaac
     5041 ggcaagactg acaggcgggt tttggggaaa gcggcgattg gatcgcctcc ctgaggcgac
     5101 tccggaaggg gaaattcatc gctgctgtcg atgatccagg cccgggcacc cactccgttc
     5161 tgacacagga aattacaggc attattatta aactccgcgc tcataggccg gaagacgctg
     5221 atcaccagat tctggtcctg aagatagatg taaggctggt caaattctat ggtgactgtg
     5281 ttttggccag cgggataatc caccatccca tcaaagacca gggtcatctg gctggacggg
     5341 atgggcccct ccgtcatatc cactctgctc gtgattccca tccagatttg ggtgggctgg
     5401 tccataaaag cgcttgcgaa gttgttgtaa aagcttagcc cgtttacaaa gccttgaaaa
     5461 tgctccagtt ctgagggata atagatggcc tgatacaacg aagacgcctg ggaaaaatcg
     5521 atgggacgac gggcttgagt ggtaccgctg ccgatcacga tgtccgtcag agcgttacat
     5581 gtcatgttga cctgaaggct ggcatccaca ggttgggtga gggggtcttt gatgtagctg
     5641 agcgtgatct gaccaggcag atcgagcgtg tcggcaggcg caaatgtgaa aagcaattcg
     5701 gcactttcac cggcttgcaa atcgattggg agcgtgagag ttggatcgat gctcagatct
     5761 ggattcccac tcagatcgac ggctgtgacc ctcaaaccgc ggccgccata attactgaga
     5821 gcgaggctct cttgaatcca tccacccgcc acggtcgtga aggtcggatt ggcagggctg
     5881 attcggagca gcggttctcc cagcgtggac gagaccaaaa acgaggtctg gggccagacg
     5941 ttggacagag agcccgcgct gaggctgtgg ggatcaaaaa cagtggtatt ggaggagttg
     6001 tggcgggtct gattggagtt tccattcatg gccataaaac cgggaggatt gcttatcgag
     6061 ggatgcaggc ccgtatggac cagcagggcc agattttgca tattatgctg ataaggatgg
     6121 gagaacagga tgtcgaaata gctggcgtcc tgggtgatca agagcggccc gtcatagacc
     6181 agattgagct gtcccgcgtt gatccatccg gtactgaggt cgttgaggtc cgtgggcccc
     6241 agccagattg tgaccggcac gttgatatca accaccgctg ggctgtggta gagccggatc
     6301 ccatggataa caccgtcgaa gttaagcaga gactgcgcct ggtagatcgt ttcgtaaacg
     6361 gcgttgccgt aatttaacga aaaaggcaga ttctgcgtca gggtgggaac tcccaaattg
     6421 agcttatcct ccaccagggc ttgcagttgg acagagactt cctgattccc cggactggtc
     6481 tgaatgctga gcgtggcggc cagcgtggta ggttctgtgg gtctgtagcg caccgtgaga
     6541 tcaaagctct ggccaaagat caggttgatc ggaacgggca gttcatcgat gctcaggtcc
     6601 gttccctggt tgaaagacgc gcccgtgata gtgagatcgc tggccccgat attgtagatc
     6661 ctgaaggtct gggaattatt gctgccaggg gccagaactc caaagtcgtg agcgctttca
     6721 tagacgtgcg gttcggggcc ttggctgaag gggttgatct gcaggcccag tttgggatag
     6781 atggggctta agttttggta ttggggattg ttggggtccc atgtactaat atatctggcg
     6841 tagcgggacc ggttggccag atcactctgg ctcatgatat tcacgcctgt ttgcagggca
     6901 ccgctgtcat agcagcgttc cagggtcaga agcagagagc cacggcaata attgaaaggt
     6961 gtatcgagcg ggatcgtcac tgtggatatg cccgtctgga tatctatgag gccgttgaat
     7021 accaggatct gatctctgac tggtacccag ccctcactga gattgcccag gtccgtgctc
     7081 tgcatccaaa tctttatttg tttgtcatac agggccacgc tgctgctagt gtagagtgtg
     7141 atctccggga tggaacccca gttcccgatt gcctggtggc tgtaaatggt ctgaaacagc
     7201 gatgcccggt aggagaaacc cagagggatg gtgctgtcga cgttaccgct tccgatcacc
     7261 tgttgatagg gctccggcag cctggcctga tagacggacc aggggctctg gtcgttgggc
     7321 tgatagctgt ctcccaccag cacgactctg gcaaagacgc tgtccactcc agcgctgggg
     7381 gccacgaaat tgatgttcag gtcgagatac tcacctggta aaacctcagt tcccggttgg
     7441 gacgcgagta tctggtttgt ggaactgacg atctggacca gataaccgga ttgagtttcc
     7501 aggcccgggt tttgcaccct caccctgccc agagaggggg atcccataaa aggctgcgga
     7561 tcagtggcta aggacactgc actgaggtca cagtcataaa tctggatgat cttaaagttg
     7621 tcgatgccat agtttgacgc tatgttcgga gcttgtaagt tgtgaaaagc cagatatatc
     7681 gtttccccgt tgtagggaaa aagactgatc tcacggcgcg cccaactgtt tagagagcat
     7741 gtgaacgatc ccagttccac agtgaaagcg ccaatgctgt tgtccgtggt ggaaagcatt
     7801 acccgatatg aatttgccga agaagaactg catcgttccc accattctat tttggcattc
     7861 acgcctgata aattgatagg tggcgtgatt agccagtcgt tgcgggccga ttgtgccacg
     7921 gccgcatagg gtggtgaggg agtggggctc agattggtgt tacccttttt ccaggtattg
     7981 tcatcggaat cgttattgac catcttccag tcaaacggtg ctgctatata gcccacccag
     8041 ggatcgtcaa aggtctcgag gtgaggaaag ctggagatca ccgaatccgg cctcacggtg
     8101 aaactccaga ccgggcagtc caaagcgtcg ccaagcgcgt tgtatggtat cacctgccag
     8161 taataggtat gcgaaaacgc cagcgaatcg ggatcgaaga gctgggctgt ccctatatcc
     8221 tgatgagctg agggaaccgg gtcctggcca aaataaagtt tgtaaccggt aggccatcca
     8281 cctccactgc tccaactcaa gatgctggtg ggatgcactc taagctcgcc atccagggga
     8341 tatgaaagca tgggtggaca tggcagcccg cttgtggtga aaggagtgta gtttatcctg
     8401 ataacgggcc ggccgcgttc gagtgttccg tctccaatga ggggagtgtg gttttggctc
     8461 cagacctgat gataatagac gggcccggct ccgtaccagg tgtaggaaac cttggggcca
     8521 aaatatccgc cacccgcggt tccagatccg ctgaccctgt aaatggtctc gaccaggacg
     8581 aggaggctca aaccggcatc gatcctgaag acggaatcca ggctgaacac ctgccagctg
     8641 ttggctgtag tgccgttcat cgtaccgctg taaacctgtg tggcaccgaa tgttatgctg
     8701 gaccacgtgc aggcttgcaa ttgggtggcg gtggtagcct tgagatagat tctcacaggc
     8761 acggcggcag tggtggtgac cccgcaatac caggccagtt catctatctg gagattgata
     8821 gcacccaact cgctgggatt gtatatcgca gcgctgcgga catacccccg ataagaactc
     8881 aaaggatggc cctggtcgtt gccagaaact gtgcccacga tcatttgctc cgcgctcaaa
     8941 gcgcagactg acaacagtat agccagtatc accagctttg tcttcatcac ttatctcccg
     9001 agaattctcc cgtcttgaag agagctttct ttgaagaaac aacaaataca tcctctgtgt
     9061 atgtgtcaag gaatgttgat cataagcctg gctctgtgga acaggttcca cacagtatcg
     9121 cctaataaca agctgctcca agacagaatt atgttgacag aatcacggct tggagggagg
     9181 atgtgaagcg ggttcagaca gaatctattt gatacctctg cctgaacgaa ggagtgtcga
     9241 tgtacagatc agttatatgc ttatggttgt ttctggctgg gatgggatcg ctgctggcga
     9301 taggatatac aagcgcgttg gtgacaacgg aggacgagtt tctcgcagcg accaagagct
     9361 ttgaggacag cgcgcctcca ccagctgttc ccaatacagt gggtctcgcg acaggaggca
     9421 tggcaccgat ggccgatact actacccgtc tggtctatgg cctcaccgat gccatcttat
     9481 cccgattcaa acaacaactc ttcatctctg cggtggtgca atggcgaaac gctctgctgg
     9541 agtctgaagt tagctatttc tttcccaatc tggcccagac gcttcagagc gttgatcccc
     9601 agagctttca gaccagctat aggctttggc agcaagccat cgagcaggac ctgatcgaga
     9661 gccccctggg catactcaat tatactgtcc aaagactgga gtcaagtaaa agcatcaaga
     9721 tcgatgagct tgctatgtta aaggagctca gggtgcttct gagtgatttt tacgcgctgc
     9781 gcaaggtgag caacctgcat gaattcatcg tgatggccac tgatggcaac agcacccact
     9841 cccccatctt cagactgatt ggccataccg ccaaaaacat gatggaccgc agcggcgaat
     9901 tagcgatagc tgatctgaat actctgtttg aaaacagcga taaacgggac cgttatatcg
     9961 agattgtcaa tcaggacatc aaggatctca ggctgtttgg agattctctg cggatcacaa
    10021 gtacgaatta tcccgcctat cagataattg cgcagacggt gagcgatttc tttgagaaaa
    10081 cagcagacag tggctcagac accatcacta cagaggaagg aagcaccgga aaactggatc
    10141 acagcaagct gatgagcctc tatctaaata cctgcgacaa ggttatggtc cagttttaca
    10201 agctggccgc tactcccaat ccccccaagt gggatatcta ctacagcacc ttcatcaaac
    10261 accggttgga cctggtggag atcgccaaag ccgctgaggc caagaactat aaagctttgc
    10321 tcaacaggat gatcgtgacg gctaccaagc tggccactcc tgatgatccc aggccgggct
    10381 cctatggcag attcctcaat ctgatcgcgg cagtcgctca gagtagtgaa gatgataaag
    10441 tgagttacag ggaattgatg aacagcgtgt tagaacccgt cggcacctat acatacaaac
    10501 gctcttccgc tttcacgttg ggaatcaatg cctatccggg cttgggagcc ggagcagaaa
    10561 tgctggaagc ggacctcgac aaggccaaag gcgtaggcgg tctggccagt cctgtgggcc
    10621 tggaactgag ctggggacgt ggattgctgg gcctcagagg gattttcttc tccgctttgg
    10681 acctcggagc aatcgccacc tatcgtttta gcggagagac agatgcgtct gtaaactcgc
    10741 ctcaggtggg ttggaagcaa ctggtctcgc ctggcgtcta tcttttggta cagggcaaga
    10801 gtcatccgct cacgctgggt tttggagctc agctcactcc tgagctcaga aaagtagaca
    10861 ctgccggcaa gcaggaagac aaaaacgcgc tgcggctggg agctttcctg agtgtggata
    10921 tcccgctttt aaccctctat gtaaaacaca actggtttaa aggagacaac tatgagagca
    10981 gagctcccag gacctggact gctgataatc ctcctgctga cagcggtttt tccactgcca
    11041 gcggaaactg agtctgtccc gcgaccgcaa tccacagacc ctgaaaggtc tggtgccttg
    11101 gtagagcagg ccctcggaat catcaatctg agtggaactg ccagagattg gagcaaggcc
    11161 gtcaacctcc tggaagacgc tgccagctta gatgacccca gagctcagta ttatctgggg
    11221 ctgaacttct acaatggtga aggggtggtg aaaaactacc gcaaagcctg cttttggttt
    11281 atgctggccg ctgcaaacga tcacgctgcc gcgtcactga gcctggaaca atgtctgcgt
    11341 aaactcagcg aggacgacgc ggaactgctg tcccagcagg tccatagttg gagacaagca
    11401 catgagacgc gttaaagaat cctggtaccg catactgctg gtgctgctga gcatcctttc
    11461 catctctctg ctggctgccg atccgctgat ggactacctg gagcgccagg ccggcgctgg
    11521 cgatcccgaa gctatgtttc tgatggggcg ctgttttgaa cgcggcgacc gggttgaaaa
    11581 gaactacgag accgccgtaa gctggtatca gaaatccgcc gggttgggac acagcggcgc
    11641 tcaaaccaac ctcggcagcc tgacgcagat gggcagagga attgctcaaa acgacagcgc
    11701 cgcggtgaag ctctttctgt tggcagccca gcagggcaat gcctttgggg cctacaatct
    11761 ggcccttagc ctggaaagag gacgggggat agaaaaagac ctgactgaag ccgtaaaatg
    11821 gtttgaagtg ggagcttcca agcggcatct gagctcgatg atcaggctgg ccggatgctt
    11881 ggagagtggc gctggaatag aaaaagatac cgataaagcc atgagctggt atcggttggc
    11941 tgccgaagcg ggatcagccg cgacgcagat gaggctgggt tcttattatg acaatgggat
    12001 ctgggtagtt tccgatcaag cggaagccct gaaatactac agaatggccg cggaacaagg
    12061 ccacgccgat gcgcagtata acctgggaat caagtacgcc cggggagaag gagtggatga
    12121 agatcaaagc caggccagct attggatttt acaagctgca cagggcggat cgccaaaagc
    12181 tcaggagctc ttagcctaca gatatctgga tgggattggc cttccccaga gtgacacgga
    12241 agcctatttc tggctgctga tcaggtctat ggatgccaac gctcccatga aatcattgcg
    12301 tgacaaaact gagaagaagc tcagccgcgc gcttgtaaat caggtgcagg aaagggccga
    12361 tcgttgggtt gagaagctca ctggtccctg agcttgatca taacgaagtc agggatccgg
    12421 gctggcatcg tcaccgggct ttgagagttc tgagtgagct ctttcacctg ggcggcgatg
    12481 tagtaatcaa gacctttgaa ggtgacctcc ttcttcccga ggaggtccgc tttccctaac
    12541 aggccttcta ccagtacttt ggtaaacgct ccattgttcc aggcaacttt ctcctgggaa
    12601 agctgagacg cggtgctgga ggcaaagacc accacactca cctcatcgct cgagaccctg
    12661 ttcaccaggc cggtggtgtc tggaagccga aaacgcggat cgtcatagat tcctcccgcg
    12721 tggcaggtat cggtgaagag gaccactttg cctgctatgg ccttgagctt ctcctccaag
    12781 acgctgtagc tcagacaggt gctttcgatg ctgtctgcat cagcatccac cgtcacaaag
    12841 tgatacatgc ctttcttatc cgcagtccca tggccggaaa gaaagatcat cgccacatcg
    12901 ctgggaccgc agagcgatgc gatccagtca aaaccctcga tcactcccct gagtgtggcc
    12961 ctggaatcag taatgatcct caccgccagg ctgtcataca tcggttttgc catcaactgg
    13021 agcgcgttcg cgaagtccga ggcgtctttg ggagggaagt tgagccgctt gatactgttt
    13081 tgataggcgg ccacccctac tgccagaaca aacaacctgg gcttgggaag cgagacaatt
    13141 ttagactcgt ctcccgccca tttcaacgat agttctttta catcaatacc cctgccgccc
    13201 tcggcgctga tcttgattct ggtattacgg ggctggagct tgagggtgaa actacccact
    13261 ccgttagtga gtagagggtc tttgatgttg agcacgctga agttatccac tttcaccttg
    13321 atggctttga ccttgtggtt ggcgggatcg gtgagcctgt aggtgaatgt gacctcgggg
    13381 ctattgtagc tggacccgtc tatgggactg agaatctcca gcctgggagg aagcgcgctc
    13441 ttgatatctt ttgagctgat ctcttcaccg gtctcttcgc ttgccagcat caaggattgt
    13501 cgcgaatcca gagtgactaa cagcgtcttc ataaggtccg gacggtagaa ctcctcccgg
    13561 aacttctcca ccggatagaa tatggagggc tgttcatagc cctgatttag ctgccagccg
    13621 atcatcgtct cgctgccggg agagcagtca taataaccct cgggagtgta gagaatccag
    13681 cgggcttgat cccggctgga aaagagcgtc atcagaagct gaccgtcctc catccggtac
    13741 cagcgaatgg tgccatccga gagggccgcc acggcaagtc tgccatcccc ggaagccttc
    13801 accgccatca ccatggcggg agcgctgacc tcccagagat aagcaccctg agcatccaga
    13861 cagtagagat tccagatcgt cccaaacagc agacgctggc ctccatccac cacgtccacg
    13921 cagagattct gctcttcatc gcgcaggaag ctcattcccc ggccgttgag cctgggtctg
    13981 aagctttggt cccaattggt gatcttgatc acgtcactgc tgtccgtaaa tgcaggatgt
    14041 gcacttggcc attcatagac gatccggtcc gccagtctga attcgcgtgg ttgagagccc
    14101 caggaagtga agcccacttc gctgccgctt gcgttgactc tcagatgcag tttgtcccag
    14161 aggttaaagt cgttgatctc aggggcgtga tacatctcta tctggttgtc cgcattcagg
    14221 ataccccaat ccggcgccat gccgcagaag aatatcccac ccttatgacg cggggccaga
    14281 tctgtgatcg agttgttgga aacactgata tcggcgtatt cacctctgcc ctgatctttc
    14341 caggccctga tcgtgtagcg cgaacctggc agactggccg cctgacaaga tccacccgcg
    14401 taaagagtct gtgaatccgc tgagaaagag gggatgaggt attcatagat ctcgcccacc
    14461 atgtcacttg tatctggttc atagagcggc ttcaggtctt tggcatccag catggccaaa
    14521 cgaaagcttt cgttaaaact caccaccagt ttgctgccgt ctgggctaaa cctgaccata
    14581 tagggtttgg aatcaccgga aaagcgatat tggctgaagc tgctgaaatc gctggagtaa
    14641 atcctcaccc gtccatcctg acaggtcaca gccaaacgtc catccccggc gaaacttaat
    14701 ccccccacac ggacggcgcc aaactccagt actgccacca gactccagtc tctgctatcc
    14761 attatgagga cctctcgggt gatcagacct acggccaggt atctgccatc gggtgaaaac
    14821 tcgatctgat tgatccggtc agccaacgcg cctttccgaa acacgatatc catggtatcc
    14881 agggcaaaaa cgtagagccc accttcctca tcatggcctc ccaccgcggc aaaacgctta
    14941 tcgggagata gtgctcccgc gtgcagacgt ccgattcgct caagcccgat ggggggtctc
    15001 aggacctggc gaagccgtcc atcgctcaaa tcccagagcc gggcactctt gtcggccgaa
    15061 ctggtgagca ggacggtctg gagcgaatcg attgagat
//
LOCUS       DAOSFN010000050        15023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3248_length_15023_cov_9.463923, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000050 DAOSFN010000000
VERSION     DAOSFN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 15023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15023
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3248_length_15023_cov_9.463923"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLO35_04700"
     CDS             <1..206
                     /locus_tag="PLO35_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH78999.1"
                     /translation="AAIENSGGAVTIRIFGRGSNIETAWVTPTAGNLPQSFLSDVKKA
                     VESWSVSGVSGPFDYEFTMRFYN"
     gene            313..2676
                     /gene="priA"
                     /locus_tag="PLO35_04705"
     CDS             313..2676
                     /gene="priA"
                     /locus_tag="PLO35_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOH79000.1"
                     /translation="MYYIIYLPVNLDKDFSYSSQRIIQPGCRVLVSFNRKDMIGISGP
                     AVDQPPAQIKCKAILEVLDDSPLISKALMDLAQWMSSYYRCPVGVCLFAMLPALLVPD
                     LSALVRLKEGVPVQEPHLASLLADGAWHSPPELRIALPKLPVLKLLEEAEVRGEIEVD
                     RRIGPRDKPRFANFVKLNPDAVNPIGLPARQAEAMTMLAASGGEMTMAALSGTFSYSV
                     IKSLTGKGLVSVIARRVDAPAAFDEYALAPKKVNLTLEQHEALDAISRGHGRFEVNLL
                     YGVTGSGKTEVYIHTMRRYLDEGFNIIFLIPEIALTPQMVERFSSEFGSVLAINHSQL
                     SDRERLRQWKLIAKGDKRIVVGARSAVFAPLPNLGLIIVDEEHEQSYKQDNQPRYHGR
                     DLAIVRAMQAGAQVIIGSATPALESWRNQQTGKYRLQRLNTRPFDIRMPGVDILDMRQ
                     EEQEELLSQTLLEAIDQRLRSKQQVILFQNRRGYSSFLQCLKCGKPIHCPACDISMYY
                     HRDTEELRCHYCGYSIPSPRKCPSCGAFSFSYGAPGTQKLEQVLKLLFPSARILRLDS
                     DSARRQDAHKGMYKGMKNRELDILVGTQMISKGLDFPGVTLVGIVLADISLNVPDFRS
                     AERTFQLLTQVAGRSGRGDLPGQVLVQTYNPEHYAIVAAAAQDYDVFAAQELEYRQRM
                     NYPPYCRLGRLLWTCASEQLITEQMDLVAMIVKPLKDNHLAIPGLSVLGPVPAPFPKI
                     GGLLRHHLILKASDPGTLKKGIDLILKDFKAPATLRLQIDIDPVSLM"
     gene            3080..3409
                     /locus_tag="PLO35_04710"
     CDS             3080..3409
                     /locus_tag="PLO35_04710"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOH79001.1"
                     /translation="MSDKDEKDLHDHAECDCGDENCECENDIITLDMEDGTQKDFHVL
                     EVIEYEGSQYIALAEVESNEYDILRMDVQGETVELAVIEDDELFEKVAQKFDEIFNAE
                     ADEEEEA"
     gene            3539..4834
                     /gene="hutI"
                     /locus_tag="PLO35_04715"
     CDS             3539..4834
                     /gene="hutI"
                     /locus_tag="PLO35_04715"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOH79002.1"
                     /translation="MKADLIIYNCSGIATLAGNPGPRTGTAMADPRIISGGAIVIRDG
                     KIAACGHESQIMASYQADQLVNARGGIVTPGFVDPHTHPVFVHTREDEFALRLEGATY
                     VEISKRGGGIRSTIASTRDADEDLLFELAKTRIKRMTALGTTTLEAKSGYGLDTASEL
                     KQLRVIKGLAEDLPIDIIPTFMGAHEYPAEYQDDHEAYLSILTEDMIPAVARQGIAVY
                     CDIFTEAHVYSVDESRRVLNCAARHGLKLKMHADEIEAIGGAELAAELHCESADHLGA
                     ASDAGINALKVAGVIPVLLPATLFSLRSKTYARGRYMIDSGLPVAIATDYNPGSCNCD
                     SMPFTMTLACLQMGLSPAEALCAATINAAHAIGLGACKGSLEPGKDADLIIWDIPSIN
                     FIPYHFGSSHISQVFAKGQLIHSPVASDADPTCTESTRG"
     gene            4840..6786
                     /locus_tag="PLO35_04720"
     CDS             4840..6786
                     /locus_tag="PLO35_04720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HOH79003.1"
                     /translation="MDQARQDHAYTILETLHRSRLHTVYHAKHYVSQEHVVIKTIDAH
                     WKSDPMLARQLRYEAELGLRLNNRHIRRTRGVFEEDGRVYVVSDYIDGKALSGILGGV
                     GISLASEMVKHWLEGLLDALDYAHSQGIVHANVNPANIIIDQDGEPVLFNFGKTLKAW
                     MNEDALQQDIHSVLFLAPEVFLGHRPDPRSDIYSIGVLAYLMLCGKLPWHVDKNVSAQ
                     SQKQTSLRQPVIDPEILGRAIPRWLYTVIHKCLIIDPNRRFQSVSELREALNAQTDLP
                     LEQIHSGPAPSDNLPPREEQFSKFAPLPQTGQPETPGSISLDFSQAQPEKVQPPQRPQ
                     PVPPPKKEAVKPIEVPKAEDTVNPELKRWGRIMAIASLVVLVFAIVKYGFFNDKPAFS
                     DKQDIESDEEGPLMDPVANVAIKMVLVQGDSTVIGTLGPDADDDEAPPVKLRVPDFWI
                     SPYEITNEQWAMVYPEHFYNLKDKDLPVTNISFLEVLEYCNEKSYKDGFDPCYDFQSG
                     YVCDFSANGYRLPTEAEWEFAAKDRKSSGFDPYSGSDDPDKAGWHSENSDGKLREVGK
                     KRANALGLHDMSGNAYEWVWNWYGPYSSRGKPFEGPSKGTDRVIRGGSYSHDVKAMRV
                     TNRSYIKQFAKSPYLGFRVARGNR"
     gene            6953..7300
                     /locus_tag="PLO35_04725"
     CDS             6953..7300
                     /locus_tag="PLO35_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOH79004.1"
                     /translation="MTLNEIANLLEGEVLNPGVDLEKEVPCAFASDLISDILMCTKEP
                     TLLLTGLTNNQVIRLADMIDLVGIIFVRGKRPLQDILEMAEERQLPVIATGMTLYRSS
                     GLLYNAGLRSCKI"
     gene            7344..7799
                     /locus_tag="PLO35_04730"
     CDS             7344..7799
                     /locus_tag="PLO35_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79005.1"
                     /translation="MKAHFASPPEVEVDLVFVVPEKDFNAAGKGSSEVKNLLKRLGVD
                     SETLRRIAVASYEAEINVAAHSKGGTMICLVYDNLIHIRFEDKGPGIWDIKQAMVPGF
                     STADDLVREFGFGAGLGLPNIEKNSDALHIVSAKDNPTLLELIIFFGTP"
     gene            7796..>8001
                     /locus_tag="PLO35_04735"
     CDS             7796..>8001
                     /locus_tag="PLO35_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH79006.1"
                     /translation="MNEKERQYFHAIQILEDNCTGCTACVRVCPTEAIRVRDRKAYID
                     STRCIDCGNCVTACQFHAIIPRSDP"
     assembly_gap    8002..8101
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8102..8818
                     /locus_tag="PLO35_04740"
     CDS             <8102..8818
                     /locus_tag="PLO35_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOH79007.1"
                     /translation="DEDLGIFLIVPCVAHVTAVHQPEGAYKHLQDGAFSIGMIYGKVR
                     EYIKDLQNNPPDIDTYPKGLTWALSGVQAELVDCEDIRTLSVNGIENVMEILSRVEDH
                     YLDQYDYIVLRSCTNGCVGGCLNVENPFVAMSRIKKMIKDGDARLIDLQGMEKLYEDG
                     EFDVAPLAARPIMELDKDIKTAIKKMKRINEFLTMLPGLNCSACGSPTCYALAEDIVQ
                     GKASIEDCVVLLKRHSKEEE"
     gene            8840..9181
                     /locus_tag="PLO35_04745"
     CDS             8840..9181
                     /locus_tag="PLO35_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOH79008.1"
                     /translation="MTKLKELLPLIKATNHTPALESADVELSGGYVCDMLSDVMGSAQ
                     PGQIWITIMKHLNVIAVASMTGIPAIIFAKGVSPDANVIDKATEEGIVLLSSPLDTFS
                     ISGIVYQAFQG"
     gene            9193..9930
                     /locus_tag="PLO35_04750"
     CDS             9193..9930
                     /locus_tag="PLO35_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOH79009.1"
                     /translation="MRWFTADLHIHSVLSPCGGLEMAPDDLIARLKELEINWFAITDH
                     NSMANCPAYHAAAKTAGLEFSWGVELQTAEEIHLLVYFDDCDAALSFDQQLYAALPSL
                     PNDPDFFGDQVVIDENCNIIRMEQKALSNSTRWDLSEAVSIASAHGGYCVPAHIDAGA
                     NSILGQLGFMPQEPDFELLGITARLDLSAFLDQHPELRHRAFLRCSDAHYLADLGSGT
                     TRLKLERASLAELFKAVRGEPRTQIIV"
     gene            9956..10438
                     /locus_tag="PLO35_04755"
     CDS             9956..10438
                     /locus_tag="PLO35_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOH79010.1"
                     /translation="MTPDTQHDNQLYAKLKDVIEQKKELRNPLIEILRVAQEIFGYLP
                     VEVQEFIAREMNIPASKVYGVVTFYNFFSMKPRGKYTLNVCMGTACYVKGAPRLVQMI
                     TDTLSTQMGETTPDGRFTISAVRCVGACSLAPVFVIGEDTFGRIETKDQIVKILERYV
                     "
     gene            10448..11017
                     /locus_tag="PLO35_04760"
     CDS             10448..11017
                     /locus_tag="PLO35_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79011.1"
                     /translation="MQDISLHLLDIIENSVRAEARNIKVRVITDAQKNRLRLIVEDDG
                     VGMDTHTVEKAQDPFYTSKLEREKKVGLGIPLFKQNAELSNGCFKMASEPGFGTVLTV
                     DFELDHIDRMPLGNLKDTLLGAVIGHPEVDFYINLIHCERGKEQSFHFDTAAIKEELG
                     DIPLTYPDVIEYIDQSLFEGIQNTNMEDV"
     gene            11017..11382
                     /locus_tag="PLO35_04765"
     CDS             11017..11382
                     /locus_tag="PLO35_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOH79012.1"
                     /translation="MKTLADLKKIREKAQEEMKLRGANVRIKIVVGMGTSGIAMGARE
                     VMKTFLEEIANRNLTDVIVTQTGEKGLSSMEPVVDLVEEGKDKVTYGNMTPEKVKKVV
                     VEHIVNGRIVSDYVVATGN"
     gene            11396..13249
                     /locus_tag="PLO35_04770"
     CDS             11396..13249
                     /locus_tag="PLO35_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOH79013.1"
                     /translation="MSLKRIDLLICCGSGCVSAGALKIKERFHTVLTEKGLQTEVNII
                     ETGCMGPCDYGPVMVIYPEGIFYKTVTPEDVDEIVEEHFIKGRPVSRLMLKDEDKTIA
                     SQKEVPFYQKQVKVALANCGYIDPESLDEYIATGGYEALGMALTQMNPQGAIDVVKAS
                     GLRGRGGGGFPTHIKWQMVHDRKEDEKYIICNGDEGDPGAFMDRSLLEGDPHRILEGM
                     MIAAYSIGASQGFFYIRAEYPLAIKRIRQAIEQAREIGLMGKNIFGTDFCFEAEVRTG
                     AGAFVCGEETALIHSIEGNRGNPTPKPPYPAVSGLWGKPSVVNNVETFANIPTIFVKG
                     AEWFASMGTATSKGTKVFALTGDIKNTGLVEVPMGITLREMIFDVGGGMVGGHKFKAV
                     QLGGPSGGCLTVEHLDVPVDYESLKERGAMMGSGGVIVMNDSNCMVNVAKFFMEFCVE
                     ESCGKCSPCRIGLKQMYDILDRITSGNGKEGDIEELQRLGESVNKLSLCGLGQSAPNP
                     VLSTIRYFRDEYESHIRDHSCGTKVCLDLMHFDIDKSRCIGCSLCARKCPVNCITGSR
                     EEKYTIHQLDCIKCGNCQDVCPVKAVDRIPGTHPEVKEQRAKQQAHNSHDD"
     gene            13261..13716
                     /gene="nuoE"
                     /locus_tag="PLO35_04775"
     CDS             13261..13716
                     /gene="nuoE"
                     /locus_tag="PLO35_04775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOH79014.1"
                     /translation="MYKEICSKYAPTKDNLIYILHDIQDTHPQHYISEEAVDAVAKYL
                     NVPPNHIYGVLTFYTMYSTSPRGKNIIRLCESPPCYIKGSENILRKMKVLLGVETGET
                     TKDGLFTLELCACLGVCGNAPVMMINEDVYGDLTEEKVEEIIDKYRRAK"
     gene            13713..>15023
                     /locus_tag="PLO35_04780"
     CDS             13713..>15023
                     /locus_tag="PLO35_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOH79015.1"
                     /translation="MKFYRSHVLVSINDTSLNAGVQDFIKALRTELAKQNLAEEINIL
                     ETGPLGFFGRGICLSVYPENVNYQDLKTEDVQELVAEHFLKGRPVSRLMLDTAGKFSP
                     KLNYANRIVLRNSGIIDPESIDEYIGAGGYEAWEKALTQMEPMQIVEEVKQSGLRGRG
                     GAGFPAGVKWSFTAPLQAEQKYVVVNADEGEPGTFKDRLIMEGDPHQLLEGIMICARA
                     VGATKAWIYIRGEYKLCIQRLEKAIAQAREYGIIGTNIFESGFDLDIDIKIGAGAYVC
                     GEETALIESLEGNRGNPRWKPPFPGVEGLWRYPTVVNNVETLANVPYIIKNGAAEYRK
                     YGTPECSGTKVFTILGDVAHPGLCEVDMGTTLRTIINDYAGGMKKGFRFKAALVGGAA
                     GVILSERHLDVKMDFASLNQYAAVLGSGAILVMNEHQSIVDMLWS"
BASE COUNT         3658 a         4119 c         3974 g         3172 t
ORIGIN      
        1 ctgccgccat cgaaaacagt ggtggcgcgg tcaccatcag aatcttcggc cgcggatcca
       61 acatcgaaac cgcgtgggtg actcctactg cagggaatct gcctcaatcc ttcctctccg
      121 acgtaaagaa agcggtggaa tcatggagtg tttcaggcgt ttccggtccc ttcgactatg
      181 aattcaccat gagattctac aactaaaccc tcaattaata gccagacata cacagctccc
      241 cctgcccagg gggagctttt tcgtgatgag gattttcatt gacaaatatg acgcttcggg
      301 ccggataagc acgtgtatta catcatctat ctgccggtaa atctggacaa ggacttcagc
      361 tacagcagcc agcgaatcat ccagcctggc tgccgggtac tcgtcagttt caaccgtaaa
      421 gacatgatcg ggatcagcgg tcccgcggtt gaccagcccc ccgctcagat caaatgcaaa
      481 gccatcctgg aagtgctgga tgatagcccc cttatctcca aagctttgat ggacctggca
      541 cagtggatga gctcttacta ccgatgcccg gtgggtgtgt gtctgtttgc catgctcccc
      601 gcattattgg tcccagatct tagcgcgctg gtgaggctca aagaaggggt cccggtgcag
      661 gaaccccatt tggcttcctt gctggcagac ggggcctggc attcaccccc ggaactcagg
      721 atcgccttgc ccaaacttcc tgtgctcaag cttctcgagg aagctgaagt cagaggagag
      781 atcgaagtgg acaggcggat tggccccaga gacaaacccc gttttgccaa ctttgtgaag
      841 ctcaatccgg acgctgtgaa ccccatcggt ctgcccgccc gccaggctga agcgatgacc
      901 atgctggccg catcgggtgg tgaaatgact atggccgccc tcagcgggac cttttcatac
      961 tctgtgatca aatcactgac cggcaaggga ttggtttctg ttattgcccg tcgggtagat
     1021 gccccagccg cgtttgatga gtatgctctg gcacccaaaa aagtaaacct taccttggag
     1081 caacacgagg ccctggatgc aatctcaagg ggccatggac gctttgaggt caacctgctc
     1141 tatggagtca ccggcagcgg caagaccgaa gtctatatcc acaccatgcg ccgctatctg
     1201 gatgagggct tcaacatcat tttcctgatc ccggagattg ctctcacacc tcagatggtg
     1261 gagcggtttt ccagcgagtt tggttccgtg ctggccatca accacagcca gctctcagac
     1321 cgtgagcgcc tgcggcaatg gaaactgatc gccaaaggag acaaacggat cgtggtgggt
     1381 gcccgcagcg ccgtgtttgc tcccctgccg aatttgggac tgatcattgt ggacgaggag
     1441 cacgaacaga gctacaagca ggacaatcag ccccgctacc atggaaggga cctggcaatc
     1501 gtgagggcca tgcaggctgg cgcgcaggtg atcatcggtt ccgccactcc agccctggaa
     1561 tcctggcgca atcagcagac cggcaagtat cgtctgcagc gtttgaacac ccgtcccttt
     1621 gatatccgca tgccgggtgt ggacatcctc gatatgcgac aggaagagca ggaagagctg
     1681 ctttctcaga ccctgctgga ggccatcgat cagcgcctga gatcaaagca gcaggtgatc
     1741 ctctttcaaa accgccgggg ttattcatcc tttttgcaat gccttaaatg cggaaaaccg
     1801 atccactgcc ccgcctgcga tatcagcatg tattatcacc gggatacgga agaactgcgt
     1861 tgtcactatt gcggttacag catccccagc ccccgtaaat gcccatcctg cggggctttc
     1921 agtttcagct atggagctcc tggcactcag aaactggaac aggtactcaa actgctgttt
     1981 ccatccgccc gcatcctgag attggattcc gattcagctc gccgtcagga tgcccataaa
     2041 ggcatgtata aaggcatgaa aaaccgcgag ctcgacatcc tggtagggac tcagatgatc
     2101 tccaagggcc tggattttcc gggtgtgacg ctggtgggca tcgtgctggc ggatatcagt
     2161 ctcaacgtgc cggatttcag atcggcggaa cgcacctttc aactgctcac gcaagtggcg
     2221 ggacgttccg gcaggggaga cctgcccggc caggtattgg tgcagaccta taatcccgag
     2281 cactatgcca tagtggcggc cgcggcccag gattacgatg tcttcgccgc tcaggagctg
     2341 gaatatcgcc agaggatgaa ctacccgcca tactgcaggc tgggacgcct cttgtggacc
     2401 tgcgcttcag agcagcttat cacggagcag atggacctgg tggccatgat tgtaaaacca
     2461 ttgaaagata atcatctggc cattccaggc ctcagcgttc tgggccccgt ccccgcgcct
     2521 ttcccaaaga ttggaggcct gctgaggcat catctcatcc tcaaggctag cgatcctggc
     2581 actcttaaga agggtatcga tctgatcctg aaggatttca aggccccagc cacgcttcgt
     2641 ctccagatcg atatcgatcc cgtatctttg atgtgatttc caccaccttc cctattacct
     2701 gatatgagca tgggcataag ctccccggac ccaaaagcca tttcaggggt ttgggccggc
     2761 tcgggtctgg ggcagaatcg ctgcagcgat caagggagca gtaacatttt caactccaaa
     2821 cacctaagcc cctaaagcct ccccggtacc tccctctgat gcctctgtta ctctgtggtg
     2881 aaacaaagta atctctgtat ctctcagtat atcatccagt tcccaatcgc cacctttaca
     2941 ccacctatgc ccctccttta catctcctat gcctcggcaa aggagaggta ttagagatgt
     3001 aatggtggcg aggcagagaa tatttagctt gccaaaagcg gcctcttcaa aaaaatggct
     3061 aaaaaacaag gagttaatca tgagcgataa agacgaaaaa gacctccatg atcacgctga
     3121 atgcgattgc ggcgatgaaa actgcgagtg cgaaaacgat atcatcaccc tcgatatgga
     3181 agatggtacc cagaaagact tccatgtgct ggaagtgatc gaatacgaag gcagccaata
     3241 cattgccctg gcggaagtgg aatcaaacga gtatgacatc ctgcggatgg acgtacaggg
     3301 cgagaccgtg gaactggctg tgatcgaaga cgatgagctg tttgagaaag tagcccagaa
     3361 gtttgacgag atcttcaacg ctgaggccga tgaggaagaa gaggcctgat ccgggctgtg
     3421 aactatcgtt ctgggcttcg gcgaccccga agcccagaat cgtttgaggg aaaaacttct
     3481 gcttgaccaa aaagccccaa gcgtaaaatt gcgtaacaag caacgattag gactacgcat
     3541 gaaggctgat ttgatcatat acaactgctc cggcatcgcc actctggccg gaaatcccgg
     3601 tcccagaacc ggaactgcga tggctgatcc caggatcatt tcggggggcg ctatcgtcat
     3661 cagagatggt aaaatagcag cttgcgggca tgaatcccaa atcatggcgt cttaccaggc
     3721 cgaccagctt gtcaacgcgc gcggaggcat tgtcactcca ggctttgtgg atcctcacac
     3781 ccatcccgtc tttgtccata ccagggaaga cgaatttgcc ctgcgtctgg aaggtgccac
     3841 atatgtggaa atttccaaac ggggaggagg catcagaagc accatcgcca gcacccgcga
     3901 tgccgatgaa gacctgctgt ttgagctggc gaaaaccaga atcaaacgta tgacagccct
     3961 ggggaccacc acgctggagg ccaagagcgg atatggactg gacaccgcca gcgagctcaa
     4021 acagctcagg gtgatcaaag gtctggctga ggatctcccc atcgatatca ttcccacttt
     4081 tatgggcgcg catgagtatc ccgcggagta tcaagacgat catgaagcct atttgagcat
     4141 cctgacggaa gatatgatcc ccgctgtggc caggcagggc attgccgtct attgtgacat
     4201 ttttaccgaa gcccacgtat acagcgtcga tgagagccgc agagtgttga actgcgcggc
     4261 ccggcatggc ctcaaactaa agatgcacgc cgatgagatc gaagccatcg gaggggctga
     4321 actggcggcc gagctgcact gtgaatcggc ggatcatctt ggagcggcca gcgatgccgg
     4381 aataaatgct ctgaaggtgg ctggagtgat cccggtcttg ttgcccgcca cgctgttttc
     4441 gttgaggtca aagacctacg ccagaggacg ttatatgata gacagtggtt taccggttgc
     4501 catcgcgaca gactacaacc ccggtagttg caactgcgat tccatgccct tcacgatgac
     4561 tctggcctgc ctgcaaatgg gcctcagtcc agccgaagcc ctctgtgccg ctactattaa
     4621 cgctgcacac gccatcggtt tgggagcttg caagggttcg ctggaaccgg gcaaggatgc
     4681 cgatctgatc atctgggata tcccttccat caatttcatc ccttaccact ttggctcctc
     4741 tcatatcagc caggtttttg ccaaaggcca gcttatccac tcacctgtag cgtcggatgc
     4801 cgatccgaca tgtaccgaat ccacaagagg atagcgcaca tggaccaagc ccgtcaggac
     4861 cacgcctaca ccattctcga aaccctgcac cgttccagac tgcacaccgt ctatcatgcc
     4921 aaacactatg tcagccagga acatgtggtg atcaaaacca tcgatgctca ttggaagagc
     4981 gatcccatgc tggcgcgtca gcttcgctat gaagcggaac tgggcctgag gctcaacaac
     5041 cgccatatcc gtcgcacccg gggtgttttt gaggaggacg gaagggtcta tgtggtatcg
     5101 gattacattg atggcaaagc tctcagcgga atccttggcg gggtggggat cagcctggcc
     5161 agcgaaatgg tgaaacactg gctggagggc ctcctggacg cgctggatta cgcgcacagc
     5221 cagggaatag tccacgccaa cgtcaatccc gccaatatca tcatcgatca ggacggcgag
     5281 ccggtgctgt tcaattttgg caagacgctc aaggcctgga tgaacgagga tgccctccaa
     5341 caggacatcc acagcgtgct gtttttggcc ccggaagtct ttctgggcca ccgtccggac
     5401 ccccggagcg atatctattc gatcggagtg ctggcttatc tgatgctatg cggcaagctg
     5461 ccctggcatg tggataaaaa cgtctccgcc caatctcaaa agcagaccag cctgcgtcag
     5521 cccgtcatcg atccggaaat cttgggacgg gcgattcccc gctggctcta taccgtgatt
     5581 cacaaatgtc tgatcatcga tcccaacagg cgtttccagt ccgtttccga gctcagggaa
     5641 gctttgaatg ctcagacgga tcttccgctc gaacagatcc actctggtcc cgctcccagt
     5701 gataatctgc cgccccgcga ggagcagttt tccaagtttg cgccccttcc tcagacaggc
     5761 cagccagaaa ccccgggcag tatctctctg gatttctctc aggcccagcc agaaaaagtc
     5821 cagccgcctc aacggcctca accagttccg cctcccaaaa aggaagcggt caagccgatc
     5881 gaagtgccca aagctgaaga taccgtgaat cccgagctca agcgctgggg caggatcatg
     5941 gccatcgcca gcctggtggt gttggtcttt gccatcgtca agtatggttt ttttaacgat
     6001 aaacccgcct tcagcgataa gcaagatatt gaatctgatg aagaaggacc gctgatggac
     6061 cccgtggcca atgtggccat taaaatggtg ctggtccagg gagattccac cgtgatcgga
     6121 actttgggcc cggatgccga cgacgatgaa gctcccccag tgaagctgag agtgccggat
     6181 ttctggatca gtccctatga gatcaccaat gaacaatggg ccatggtgta tcctgagcac
     6241 ttttacaact tgaaagacaa agatctgcca gtgaccaata tcagctttct ggaagtgctg
     6301 gagtattgca acgaaaagag ttataaagat gggtttgacc cgtgttatga cttccagagt
     6361 ggctatgtgt gcgatttttc ggccaatggc tatcgcctgc ccactgaagc ggaatgggaa
     6421 ttcgcggcca aagaccgtaa gagttcaggg tttgatccct acagcggatc cgatgatccc
     6481 gacaaagccg gctggcactc cgaaaacagc gacgggaagc tgagagaggt gggcaaaaaa
     6541 cgggctaatg ctctgggcct ccatgatatg agcggaaacg cctacgaatg ggtctggaac
     6601 tggtatggcc cctatagctc caggggaaaa cccttcgaag gcccctccaa ggggacggac
     6661 agagtgatcc gcggcgggtc ttacagccat gacgtcaaag ccatgagggt cactaaccgc
     6721 agctatatca agcagtttgc caagagtccc tatctggggt tcagggtcgc tcgcggcaac
     6781 cgctgatcca agtatctgct tttcataagt tccagggcag cagaattgct ctcctttggg
     6841 gttccaatgc ctaccgtcaa actgggcatt cttattgaca ggcaagtatt tccttgactt
     6901 aaatgcctct ttcccagagc tgtcgctcta caaacaaaac aggtgaattc gaatgacctt
     6961 aaacgaaata gcaaaccttt tagagggtga ggtcctgaat cccggagtgg acctcgagaa
     7021 agaagtgcct tgtgccttcg cgagcgattt gatcagcgat atcctgatgt gcaccaagga
     7081 gcccaccttg ctgctaacgg gactcacgaa caaccaggtg atccgtttgg ccgatatgat
     7141 cgatctggtg ggaatcatct tcgtgagagg caaaagacct ctccaggaca tccttgagat
     7201 ggctgaggaa cgtcaactgc cagtaatcgc tacgggcatg accctgtatc gctccagcgg
     7261 tttgctttac aatgccggat tgcgtagctg taaaatttga aatggcagaa tactggcata
     7321 tagttcccca atttgaggag gctgtgaagg ctcacttcgc ctctcctccc gaagtggaag
     7381 ttgatcttgt gtttgtagtc ccagaaaagg atttcaacgc cgccgggaag ggctcatcgg
     7441 aagtgaagaa tctgctcaaa cggctgggag ttgattctga aacgctgcgc cggatagcgg
     7501 ttgcctctta tgaagcagag atcaacgtgg ctgcccactc caagggtggc actatgatct
     7561 gtctcgttta cgataacttg atccatattc gttttgaaga taagggccca ggcatctggg
     7621 acatcaaaca ggccatggtg ccaggttttt ccacggcgga tgatctggtg agggagtttg
     7681 gctttggggc cggcctggga ttgccgaaca tagaaaagaa ttcggacgcg ctgcacatcg
     7741 tgtccgccaa agacaatccc actctcctgg aactgataat attctttggt acaccatgaa
     7801 cgagaaggaa agacaatact tccacgccat tcagatcctc gaggacaact gcaccggctg
     7861 cacagcctgc gtgagagtgt gccccaccga agcgatccgg gtgcgtgacc gcaaagccta
     7921 tattgattcc acccgctgca tcgactgcgg taactgtgtg accgcgtgtc agtttcatgc
     7981 catcattcca cgcagcgatc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 ngatgaggac ctgggtatct ttctgatcgt accctgcgtg gcccatgtga ctgccgttca
     8161 ccagccggaa ggagcttata agcacctgca ggatggagct ttttccattg gcatgatcta
     8221 cggcaaagtg cgtgagtata tcaaagacct tcagaacaat cctccggata ttgataccta
     8281 ccccaaaggc ctcacctggg ccctctctgg agtccaggct gaactggtgg attgcgaaga
     8341 catccgcacc ctctcagtga atggcatcga aaacgtgatg gagatccttt ccagagtgga
     8401 agatcactat ctggaccagt atgattacat cgtgttgcgc agctgcacca atggctgcgt
     8461 gggcggatgc ctcaacgtgg aaaatccctt cgtggccatg agccgcatca agaaaatgat
     8521 caaggatggt gatgctcgcc tcatcgacct tcagggcatg gaaaagctct atgaagatgg
     8581 tgagtttgat gtggcaccgc tggccgcccg tcccatcatg gaacttgata aagacatcaa
     8641 gaccgctatc aagaagatga aacgcatcaa tgagtttctc accatgctgc cgggtttgaa
     8701 ctgcagcgcc tgcggttctc ccacctgcta tgccctggca gaggatatcg tgcagggcaa
     8761 agcctccatc gaagactgcg tggtgctgct caagcgccac tccaaagaag aagaataaag
     8821 acaaacagga tatagattta tgaccaagct caaggaactg ctgccgctca ttaaggccac
     8881 caatcacact cccgccctgg aatctgccga tgtggaacta tccggcggtt acgtctgcga
     8941 tatgcttagt gacgtgatgg gatcagccca gccgggccag atctggatca cgatcatgaa
     9001 gcacctcaac gtgatagccg ttgcttccat gacgggtata ccggccatta tcttcgccaa
     9061 aggcgtgagc ccggatgcca atgtgatcga taaggccacc gaagagggta ttgtgctgct
     9121 gagcagccct ctggatactt tcagcatcag cgggatcgtc tatcaggcct ttcaaggctg
     9181 atcactcagt ccatgcgatg gttcaccgcc gatctccata ttcactcagt cctctctccc
     9241 tgcgggggat tggaaatggc gccggacgat ctgatcgcca ggctcaaaga actggagatc
     9301 aactggtttg ccattacgga ccacaacagt atggccaact gcccagccta tcatgctgcc
     9361 gcgaaaaccg ctggattgga attcagctgg ggagtagagt tgcagaccgc tgaagagatc
     9421 catttgctgg tctatttcga cgattgtgac gcggctctca gctttgatca acagctctac
     9481 gcggccttgc cttcgcttcc caatgatccg gacttctttg gggatcaagt tgttattgac
     9541 gaaaactgca acattataag aatggaacaa aaggcgctat ccaattccac ccggtgggac
     9601 ctcagcgaag cggtctctat cgccagcgct catggcggct attgcgtgcc cgctcatatc
     9661 gacgcgggtg ccaacagcat cctgggccag ctgggattta tgcctcagga gcccgatttt
     9721 gagctgctgg gaatcaccgc gcgcttggat ctttccgcgt ttctggatca gcatccggag
     9781 ctgcggcaca gggcctttct gcgctgttcc gatgcccatt acctggcaga ccttggttcc
     9841 gggaccacgc gtctcaagct tgaaagagct tcgctcgcgg aactttttaa ggccgtccgg
     9901 ggcgagcctc gcacccaaat catagtataa aaataaagat aagcaaggag gagtgatgac
     9961 tcccgatacc cagcacgaca accagcttta cgccaagctc aaggacgtga tcgagcagaa
    10021 gaaagagctg cgcaatcccc tcatcgaaat cctgagagtg gcacaggaga tctttggcta
    10081 cctgcccgtc gaagtgcagg aattcatcgc ccgcgagatg aacatccccg ccagcaaagt
    10141 ctatggcgtg gttacctttt acaacttctt ttcgatgaaa ccccgcggca aatataccct
    10201 caacgtctgc atgggaactg cctgctacgt gaaaggcgcg ccaagactgg tgcagatgat
    10261 caccgatacc ctgagcaccc agatgggtga gaccactccc gacggccgtt tcaccattag
    10321 cgctgtaaga tgcgtgggcg cttgttctct ggccccggtg tttgtgatcg gggaagacac
    10381 ctttggccgc atcgagacca aagaccagat tgtcaaaatc ctcgagcgct acgtctaacg
    10441 gcccgccatg caagacatct ccctgcacct tctggatatc atcgaaaact ccgtgcgagc
    10501 ggaggccaga aacatcaagg tgcgcgtgat caccgacgct cagaagaacc gtctgcgctt
    10561 gatcgttgaa gacgacggag tgggtatgga cacccacacg gtggaaaaag cccaggatcc
    10621 cttttacacc agcaagctcg aacgcgagaa aaaggtgggg ctgggcatcc cgctcttcaa
    10681 gcaaaacgcc gagctttcca acggctgttt caagatggct tcagagccag gttttggaac
    10741 agtgctcacg gtggattttg agctggacca catcgaccgc atgccgctgg gcaacctcaa
    10801 agacaccctt ctgggcgctg ttatcggcca cccggaagtg gatttctata tcaacctcat
    10861 ccactgcgaa cgaggcaaag aacagagctt tcacttcgac acggccgcca tcaaagaaga
    10921 gctgggagat atcccgctca cctatccgga cgtgatcgaa tacatagacc aaagtttatt
    10981 tgaaggaata caaaatacca acatggagga tgtctgatga agacacttgc cgaccttaag
    11041 aagatccgcg aaaaagctca ggaagaaatg aagctccgcg gcgcaaacgt acgtatcaag
    11101 atcgtggtgg gaatgggaac ttccgggatc gccatgggcg cccgcgaagt gatgaagacc
    11161 ttcctggaag agatcgccaa ccgcaacctt acagacgtta tcgtcaccca aaccggtgaa
    11221 aagggccttt cgtccatgga accggtggtg gacctcgtgg aagagggcaa agacaaggtg
    11281 acctatggca acatgacccc cgaaaaggtc aagaaggtgg tagtcgagca tatcgtcaat
    11341 ggaagaatcg tctccgatta tgtagtggcc acaggcaatt gagaggagga accgaatgtc
    11401 tctcaaaaga attgacctgc tgatctgctg cggctcaggc tgcgtatcgg caggggcctt
    11461 gaagatcaag gaaagattcc acacagtgct cactgagaaa ggcctgcaaa ccgaggtcaa
    11521 tatcatcgag accggctgca tggggccctg tgattacggt cccgtgatgg tgatctatcc
    11581 tgaaggtatc ttctacaaga cggtcactcc cgaagacgtg gatgagatcg tggaagagca
    11641 ctttatcaag ggccgtcccg tttcccgtct gatgctaaag gacgaggaca agaccatcgc
    11701 gtcccagaaa gaagtgccat tctatcagaa acaggtgaag gtagccctgg caaactgcgg
    11761 ctacatcgat cctgaaagcc tggatgaata tattgccacc ggtggttatg aagccctcgg
    11821 catggccctc acccagatga atcctcaagg cgccatcgac gtggtcaaag cttccgggct
    11881 cagaggtcgt ggcggcggtg gattccccac tcacatcaag tggcagatgg tgcacgaccg
    11941 caaagaagac gagaaatata tcatctgcaa cggtgacgaa ggagacccgg gcgcgttcat
    12001 ggaccgttcc ctcctggaag gtgatcctca ccgcatcctg gaagggatga tgatcgccgc
    12061 ctattccatc ggtgccagcc agggattctt ctacatccgc gccgaatatc cgcttgccat
    12121 caaacgcatc cgtcaggcca tcgaacaagc ccgtgagatt ggactgatgg gcaagaacat
    12181 ctttggcacc gacttctgct ttgaagccga agtacgcaca ggtgccggtg ccttcgtttg
    12241 cggtgaagag actgctctca tccactccat cgaaggcaat cgtggaaacc ccacacccaa
    12301 gcctccctat ccagccgtca gcggcttgtg gggcaaaccc tccgtggtca acaacgtgga
    12361 gacttttgcc aacatcccca ccatctttgt gaagggggct gagtggtttg cttccatggg
    12421 aaccgccacc agcaaaggca ccaaggtctt tgccctcacc ggtgacatta aaaacaccgg
    12481 cctcgtggaa gtgcccatgg gcatcaccct gcgcgaaatg atctttgacg tgggcggtgg
    12541 catggttggc ggtcacaagt tcaaggccgt ccaactgggt ggcccctcag gtggctgcct
    12601 cacggtcgag catctggacg tcccggtgga ttatgaaagc ctcaaagaac gcggtgccat
    12661 gatgggttca ggcggtgtga tcgtgatgaa cgacagcaac tgcatggtca acgtggccaa
    12721 gttctttatg gagttctgtg tggaggaatc ctgcggaaag tgttcaccct gtcgcatcgg
    12781 gctcaaacag atgtacgaca tcctggaccg catcacttcc ggaaatggca aggaaggcga
    12841 tatagaggaa ctgcagcgcc tgggcgaaag cgtcaacaaa ctctccctct gcggtttggg
    12901 ccaatcggcc cccaacccgg tgctctccac tatccgctat ttccgtgatg aatatgaaag
    12961 ccacatccgc gatcacagct gcggtaccaa ggtttgcctc gatctcatgc attttgacat
    13021 cgacaaatcc cgctgcatcg gttgctcgct gtgcgcccgc aagtgtcctg tgaactgcat
    13081 caccggctct cgcgaggaga aatacaccat ccatcagctt gactgcatca aatgcggaaa
    13141 ctgccaggat gtatgtcccg tcaaggccgt ggacagaatt cccggcacgc atcccgaagt
    13201 taaagagcaa cgcgccaaac agcaagctca caacagtcat gacgattaaa aggggaatac
    13261 atgtataaag aaatatgctc gaaatacgct cccaccaaag acaacctgat ctacatcctg
    13321 catgatattc aggataccca ccctcagcac tacatctctg aggaagcggt ggacgcggta
    13381 gcaaaatatc taaacgtgcc gcccaatcac atttacggcg tgctgacctt ttacaccatg
    13441 tacagcacca gcccgcgtgg aaaaaacatc atccgcctct gtgaatcccc gccctgctat
    13501 atcaagggta gcgaaaacat cctccgcaag atgaaagtcc tgctgggtgt cgagaccggg
    13561 gaaaccacca aggacggcct tttcactctt gagctctgtg cctgcctggg tgtgtgtgga
    13621 aacgctcccg tgatgatgat caatgaagac gtctatggcg atctcaccga agagaaagtg
    13681 gaagagatca tcgacaaata caggagggcc aaatgaagtt ctatcgcagt cacgtcctcg
    13741 taagtatcaa tgacacctcc ctcaatgccg gagtgcagga ttttatcaaa gcactcagga
    13801 cagagctggc caaacagaac ctcgccgaag agatcaatat cctggaaaca ggtccgctgg
    13861 gtttctttgg acgcggcatc tgtctctccg tctatcctga aaacgtcaac tatcaggacc
    13921 tcaagaccga agacgttcag gaactggtgg cggagcactt cctcaagggc cgtcccgtga
    13981 gccgtctgat gctggacacg gcaggcaaat tcagccccaa actcaactat gccaaccgca
    14041 tcgtgctgcg caactccggt atcatcgatc ccgaaagcat cgacgaatat atcggtgccg
    14101 gtggatatga agcctgggaa aaagccctca cccagatgga accgatgcag atcgtggaag
    14161 aagtcaagca gtccggcctg cgtggtcgtg gcggtgcagg cttcccggct ggagtgaaat
    14221 ggagcttcac cgctcctctg caggccgaac agaaatacgt ggtcgtcaat gccgacgaag
    14281 gcgaacccgg cactttcaaa gatcgtctga tcatggaagg cgatccgcat cagttgctgg
    14341 aaggcatcat gatctgcgct cgtgccgtgg gggccaccaa agcctggatc tatatccgcg
    14401 gcgaatacaa gctctgcatc cagcggctcg aaaaggctat cgctcaggcc cgcgagtatg
    14461 gcatcatcgg caccaacatc ttcgaaagcg gctttgacct ggatatcgac atcaagatcg
    14521 gtgccggcgc ttatgtctgc ggagaagaaa ctgccctgat cgaatccctg gaaggcaacc
    14581 gcggcaatcc cagatggaaa ccacccttcc ctggcgtgga aggcctctgg agatatccca
    14641 ccgtggtcaa caacgtggag accctggcca acgtgcctta catcatcaaa aacggcgctg
    14701 ccgaataccg caagtatggc actccggaat gcagtggcac caaggtcttt accatccttg
    14761 gcgatgtagc ccatcccggc ctctgtgaag tggatatggg caccaccctg cgcaccatca
    14821 tcaacgacta tgccggtggc atgaaaaaag gcttccgctt caaagccgcc ctcgtgggtg
    14881 gagcagcggg cgtgatcctt tctgaacgcc atctggatgt caagatggac tttgccagcc
    14941 tcaaccaata tgccgcggtg ctcggttccg gtgccatcct ggtgatgaac gagcatcaga
    15001 gcatcgtgga tatgctgtgg tcc
//
LOCUS       DAOSFN010000051        14911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3293_length_14911_cov_8.640347, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000051 DAOSFN010000000
VERSION     DAOSFN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 14911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14911
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3293_length_14911_cov_8.640347"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4058)
                     /locus_tag="PLO35_04785"
     CDS             complement(<1..4058)
                     /locus_tag="PLO35_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOH79016.1"
                     /translation="MTAPTARLIMVFTLLLCTAAAFGISFDYGKQWKEIDALLGKGLP
                     VSADARIDSLLAAAYSDGIVEQQIKGLVYKLTILQKKEEFSAQKAIDAVRLRLESARF
                     PSSAILHSMLAQLYWSYYQSNRYLFSNRTQTTNFQRDDIATWDLATIAEETIKAYRLS
                     LQRPQDLQTISIWDYPAIINKGGADEKQLRPTLYDFLAWRALDFYVNDESGLTRPPDE
                     FKLDKSGYFSSASSFAAMSIDSPDSLSLNYLAVNLFQDLIGFHLADSDPAARMEANLD
                     RLSFVYRKSSLADKASLYEKALRLLLQDYSANPASALASYRLALLYQSLAASFKTELS
                     EEHRWDLKTAAEICQDAMKRFPSSYGARACRALYNTLNSRELSISGEDLVIPNTPVKV
                     LVSYKNIPRLEIRIYRIPYKDIQDRYTSEIWDWQNDKKTKLYSQLRKKAVWKQSFDLL
                     DEGDYRTHRYELALAKLPAGHYIMIAADGDLDRIEGKVLGFSLFSCTSISNITPHSEN
                     NTIHLANRDSGWPISGAQVRVYGDDNNNRQSVLAWSGSSDVDGMVRVEGDRGLWNKKM
                     EISTGADTLQIWSYSSSNPENSARLSGQCLLFTDRAIYRPGQTIHVKGVVYYTDGEKR
                     SALAPQSDVTLSFKDVNYQQIDSQKLKANEYGCFATSFIAPTGVLTGNMSIQAFFTIK
                     TNRDETGIAGLGGSSSTSISVEQYKRPRFEVKLDQPTQTYALNQSVDLKGKAVAYSGV
                     PIDNSAYSYRIYRQAQFPYWYWWWGPRPSSPEKEVASGTGSTDAMGEFSLSFIASADA
                     EALPAYNPYFTYRITVDVTDLNGETRSGALSLNVGMNELILGVELAAQIDIQADGLKL
                     PIQTSNLNGAPIAAKGKLTVSRLQAPDHIQKSRLWSAPDRNYLSREDFVKAFPNDPYF
                     TEDRINTWPVLEKVWTSDFSTPERGDVSVENLSRWQPGAYVLEAIASHKGKEVKVLRY
                     FTVYDSRSKDLPYPQTDWLVPVKTVCQPGETAKVLFGSSYAKVRLRYEVEKNHALAQS
                     EILVLNSEQRMFEVPVTEADRGGFYIHFTFIKDGRAYLSSQEISVPWTNKQLSIEYQS
                     FRDKLLPGNQEEWRLKLKDHTGGRVTAEVLASMYDASLDAFRSSTWTANIWKKIPKTS
                     GWHSGNFVNRADLYLVQYHNLDAYIPPRSFDSFNWYGYSLHYSRNWGVKMAADKVSVG
                     SSREIISDSLSEPSVSDMANVVSLQTAVAPPVSSPKPVDLAQVAARSNFAETAFFYPT
                     LRTDANGEVSFVFTVPDALTRWKFRAFALSSDLSIGLTESSAVTQKPLMVQPNLPRFF
                     REGDRISVSAKISALEDKDLKGKCQLFLLDAFT"
     gene            complement(4344..4742)
                     /locus_tag="PLO35_04790"
     CDS             complement(4344..4742)
                     /locus_tag="PLO35_04790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOH79017.1"
                     /translation="MIANFRKRIYGYECDLYGHLHNTNYLNILEAARSEALVDVGMPV
                     KRLMELNWHVYINKIEVEFLKGVGVDDIIEVRSQIYDRNKLRSSWNQEIYNEAGELCF
                     RAQIHVVHIHNGKPARVPEHIWEHFKLLGE"
     gene            complement(4812..8132)
                     /locus_tag="PLO35_04795"
     CDS             complement(4812..8132)
                     /locus_tag="PLO35_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH79018.1"
                     /translation="MIEVILNGQKVETEPGTSILDLARAHGCEIPTLCHDEELKPYGS
                     CWVCAVEVKGRRGFVTSCGTLVSPGMEIVTDSPEIHAARKMALELLISNHYADCEAPC
                     KIACPDHVDIQSYVSLIANGQYHEAVKVIKDTLPMPLSIGRVCPAFCEKECRRQIVEE
                     PVAIRQLKRYAADEDLGDVWNYIPEKEADTGKKIAIIGAGPSGLTCGYYLSNQGHAVT
                     VFEAAPQAGGWLRYGIPEYRLPKATLDAEIELMCSNGMKIEYGKALGKDLSLKDLAAA
                     YDAVYLALGAQKAVPMPVKGSDLQGCYLGVDFLKAHALGDTPHLGKKVAIVGGGNTAI
                     DCARTAIRLGCEVALIYRRTREEMPAEPFEIEAAGHEGTVFHYLCNPVEYFGEDGKLK
                     SVKIEKMKLGDPDSSGRRRPEPTGEFFTQEFDSIIAAISQVPEVDLFADDPNKIDGQI
                     IPISRWQTAIVDEGSMHTGLANVFAGGDFRRGAATAIEAIADGRAAAEAIHRYLNGLA
                     LPPARPKFDSKKGKKISEISAKEYSLFEKIARFKMPEIPIPQAKTSFDEVETGFTETD
                     ARAEADRCLECGCQVNETCALREYCTDYEVNALSFPGSINKHPIDYSHPYIVRDANKC
                     INCGRCVRTCAEIQGPAVLGYIYRGFAAVVAPEFGESLTQTTCESCGKCIAVCPVGAL
                     VERNLHYKLNPLPKTETLQNCGLCGTGCYIKVEDQSGVVTRIETPSEDDLQGFNGRDL
                     CFKGRFGWQVLLSEDRLRTPRAKENGVWRDISWQEAKELIASKATTAQKKRFDVSASL
                     AIEELLIAKKAAESCGARLQADPCCLPLGPESYFVAPDPFPFARLDEFEEYVVCGEID
                     LVTRTRLRLKQRIGKRLIFVGKSEAPWLRFADAVYPRLCDLPEASSRLYVYNQEQLSA
                     REAYGVWKLAALDKVYPRNVMAGSDLPNLGGLIAIEPCFNLPSTADFVIAWGTGLRDQ
                     DSSAFRTSIQLYKDDDSKADLLLPASSYLEIDGSTLGNNGAVSFFRDPAKANHICELM
                     KLLYELGWINPATADINHWNTLATELLDGLKSKPVKVFDFRNVDPDKLDASPRAIKTE
                     LQSRMTALHEDRKTPVTF"
     gene            complement(8143..9912)
                     /gene="nuoF"
                     /locus_tag="PLO35_04800"
     CDS             complement(8143..9912)
                     /gene="nuoF"
                     /locus_tag="PLO35_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014122564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOH79019.1"
                     /translation="MQAITVKVGLASCGIAAGADEVYRKLENYLMENVRPVKLQRTAC
                     IGMCFEEPIVELSGSEAGSVTLGKVTADNIATLVEEYLAGGVPSSHVILAQKYDAPRN
                     ALLANQKRIVLQNCGVIDPKSLEDYEASGGYQALRKALTMSGDEIITEVKESGLRGRG
                     GAGFSTGLKWSFAKNAQGPKKYVVCNADEGDPGAFMDRSVLEGDPHKIIEGMVIGAYA
                     MGADEGYIYCRAEYPLAIAHLKVAIEAAEQKNYLGKHILGSDFSFELHIKEGAGAFVC
                     GEETALMQSIEGKRGMPTIRPPFPAESGLWGKPTNINNVETWANITWIILNGAKAFHA
                     MGTEKSPGTKVFALAGKIAGSGLIEVPMGITIRDIIYKVGGGMKTEKPFKAVQLGGPS
                     GGCIPAELLDTTVDYDSINATGAIMGSGGMVVMDSGTCMVDVARYFLNFTQNESCGKC
                     TFCRIGTRRMLEILTRITEGKGVMQDIERLEDLATNIIKGSLCGLGQTAPNPVLTTLR
                     YFKHEYIAHIQEKRCPAGACTALLKYEIIPEKCVGCTACARKCPVNCISGSVKQPHVI
                     DQDKCIKCGACYKTCKFNAIVKG"
     gene            complement(9922..10386)
                     /gene="nuoE"
                     /locus_tag="PLO35_04805"
     CDS             complement(9922..10386)
                     /gene="nuoE"
                     /locus_tag="PLO35_04805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOH79020.1"
                     /translation="MQKDFVKLDALLERYRGKKGILIPMMQEVQDLEGFLSKDTMRYI
                     AAKMDIPAAEIFGVATFYSMFRLKAQGKHIIRVCKGTACHVSDADGIKDALVEHLKLP
                     DGENTTSDMRFTLMEVACLGCCSLAPVIMVDDQTYGKLTPEAIPGILEKYSF"
     gene            complement(10578..11378)
                     /locus_tag="PLO35_04810"
     CDS             complement(10578..11378)
                     /locus_tag="PLO35_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH79021.1"
                     /translation="MKRFLTLVLLLSLTLVSLSAQKALVIGNSGYASKALPQPARDAA
                     LIDSTLAQSGWTVTNHQNLGPLALKTALSQFNNTLAEGEMAMVYFSGAVIQLDGLNYL
                     VPVGIFKDAATFKTSAVELNWAISQLSKAGVKLIFLDGARSPANIGFKLAKPGMAALA
                     KLPGNTLLMYGSPLDTVLVDSPAPNSYFSKALAAEIIKPDLDLSVLPANISAAMAILQ
                     GGKTPPQPWSASSVPEGWTLNPSGENMPRYHFRGMFKMDIDGGGSYSF"
     gene            complement(11398..14286)
                     /locus_tag="PLO35_04815"
     CDS             complement(11398..14286)
                     /locus_tag="PLO35_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOH79022.1"
                     /translation="MTDDMIFGPGFFGLEEDNLTQKRKLQLREGELREVTVLFADIKG
                     FTNLSSRLDPEVIHARMDELLKVFSRCVTFYGGFVDKYIGDAIMALFGAKKASEQDTE
                     RAIRAGLKMLEQLQLYNHNLRQMPGYEDVELSVRIGINTGLVSVGRVGESREGDFTVY
                     GPEVNLASRMESNAPVGRIMVPDTTKKLVEQIFEFEPLGLKQVKGIDKAVDCWSPLGL
                     KTLEASLAERYGSSFIGRETELRALEEALDLIIAQTGKISPQAELPAPRPVIIGIRAD
                     AGIGKSRLVYEFRRSNSDKAEFIVAGCDGVSPTPLHLFARLLEQRFGLRTTEPVDAKL
                     AKLQAAFSALEATAEVELRSSLQDAFPLIAWLLEIRVADPRLKQGGKDLLQHLLLAVN
                     TVLEAVLVQCAQMGKPLVLVLDDLHWLDDSSSAVLEHLVNRFSQSKLPSLWLLPYRPE
                     FDPPSYLARMRGFNELSLEPLDEKSITELVLKHTRHLDLSERSIEQVVSLSAGNPFYL
                     EEWCNYVSSLGETQWQDMPVPASLNALILSRLDSLPQVLRVLLQKAAVIGQEFFVEIL
                     REVERNLEDPVDVDATLATLEDHSLIIKLLGFEFSSYLFKHITTREVAYQTLLITNRK
                     VLHELTAEAIEALFPDRLDDFSFNLGTHYLKAELPQKALPWLRKAADSAARVFDNNRA
                     LDLYCQLLDLLPQENYQQRGDILLQCIEIRWLTGAWKSLGADLDALNELGLKAGNPEL
                     CFHAQRLRGMIAFYKRQRDEAKVCWDQAAELAGRIENLMPRCMIENLMGIWYQDGGLW
                     DQALERHQSSIALARSLADPQREAKSLNNIGLLHLARQEYEPALEAFQQSLELAQNHR
                     YLKDESLAIGNLGHANIVMGRMNEAMPYLEKKLELANRMNDKIETIKALGNMGRVHAA
                     LQEYDKAIACYERIIRIKQYLGDENGIKDTTEVLEAIKASAAAPKQHQ"
BASE COUNT         3174 a         4049 c         4108 g         3580 t
ORIGIN      
        1 gtaaaggcat ccagcagaaa gagctggcac ttgcccttga ggtctttgtc ttccaaagcg
       61 ctgatcttgg cagacacgct gatccggtct ccttctctga aaaagcgggg cagattgggc
      121 tggaccatca aaggtttctg cgtgacggca gaggactccg tgagcccgat gctgagatcg
      181 gaactgaggg caaaagcgcg gaatttccag cgggtgaggg catccggaac ggtaaagaca
      241 aagctcactt ccccgttggc atcggtgcgc agagtgggat agaaaaaggc cgtctcggca
      301 aagttggacc gggctgccac ctgcgccaga tcaaccggtt tgggagagct aaccggcggc
      361 gcgacagcgg tttgcaagga tacaacattg gccatgtcgc tcactgatgg ttcagacagg
      421 ctatccgata tgatttccct ggagctgccc actgaaactt tatcagccgc catttttacg
      481 ccccaattgc ggctgtaatg cagcgaatag ccataccagt tgaagctgtc aaagctgcgg
      541 ggagggatgt aggcatccag attgtgatac tgcaccagat acaagtccgc cctgttcaca
      601 aagttgccgg agtgccagcc gctggttttg gggatctttt tccagatgtt ggccgtccag
      661 gtgctggagc ggaaggcatc cagcgaagcg tcgtacatgg aagccaacac ttccgcggtg
      721 acgcgtccgc ctgtgtggtc cttgagcttg aggcgccact cttcctgatt gccgggcaag
      781 agtttatctc taaagctctg gtattcgatg ctgagctgct tattggtcca gggtacgctg
      841 atttcctggc tgctgagata ggcccgcccg tctttgataa aggtgaaatg gatgtagaag
      901 ccaccccggt ccgcttccgt gaccgggacc tcgaacatac gctgttcgct gttgaggacc
      961 aggatttcgc tctgtgccag agcatggttt ttctccactt catagcgtag ccggaccttg
     1021 gcataactgc tgccaaacag cactttagcc gtctcgccgg gctgacagac cgtcttaacc
     1081 ggcaccagcc agtcagtctg aggatagggc aggtcttttg agcgggagtc atagacagtg
     1141 aagtagcgca acaccttgac ctctttgccc ttatggctgg caatggcctc cagcacataa
     1201 gctcccggct gccatctgct taggttttct acgcttacat caccgcgctc tggggtgcta
     1261 aagtctgagg tccacacttt ttccagcacc ggccaggtgt tgatgcggtc ttctgtaaag
     1321 tagggatcat tggggaaggc cttcacaaaa tcctcccggc tcagatagtt gcggtcggga
     1381 gcgctccaga gccggctttt ttgaatgtgg tccggggctt gaagccttga gaccgtcagc
     1441 ttccccttgg cggcaatcgg tgctccgttg agattactgg tctggatagg gagcttgagg
     1501 ccatccgcct ggatatcaat ctgcgccgca agttccactc ccagtatcag ttcattcatt
     1561 ccaacgttga ggctcaaagc accgctgcgg gtctcgccgt tgaggtctgt gacgtccacc
     1621 gtgatcctgt aggtgaaata gggattgtag gccggcaggg cttccgcgtc cgccgaagcg
     1681 atgaaggaaa ggctaaactc acccatcgcg tccgtgctgc ccgtcccgct ggcaacttcc
     1741 ttttcagggc tggaaggcct ggggccccac caccaatacc aatagggaaa ctgcgcctga
     1801 cgatagatcc tgtagctgta agcggaattg tcgatcggca ctccggaata ggctacggcc
     1861 ttgcctttga gatccacgct ctggttgaga gcgtaggtct gagtgggctg gtccagcttg
     1921 acttcaaagc ggggacgctt gtattgttca acgctgatgc tggtactgct gctaccgcca
     1981 agacccgcaa ttcctgtttc atcacggttg gtcttgatgg tgaaaaaagc ctggatgctc
     2041 atatttccag tcaatactcc cgtaggcgcg ataaagctgg tggcaaagca gccgtattcg
     2101 ttggccttga gcttctgaga gtcgatctgc tgatagttta catctttaaa gctaagcgtt
     2161 acatcgcttt gaggtgccag agcgctgcgc ttttcgccat cggtgtaata gactacaccc
     2221 ttcacatgga tagtctggcc gggacggtaa atggcgcggt ctgtgaacag cagacactgg
     2281 ccagacagcc tggcggaatt ttcgggattg gagctggaat aagaccagat ctgcagcgtg
     2341 tccgcccctg tgctgatctc catcttcttg ttccacagcc ctctatcacc ctccacgcga
     2401 accattccat cgacgtcgct ggagccgctc caggcgagaa cgctttggcg gttattgtta
     2461 tcatccccat aaactctcac ctgagcccca gagatcggcc agccgctgtc ccgattggca
     2521 agatggatgg tattgttctc ggagtgggga gtgatgttgc tgatcgatgt gcaactaaag
     2581 aggctgaaac ccagcacttt accttcgatt ctatccaggt ctccgtccgc cgcgatcatg
     2641 atatagtgtc cggccggcag ctttgccaga gcaagctcat atctgtgggt acggtaatca
     2701 ccttcgtcca gcagatcaaa gctctgtttc cacacagctt tctttctgag ctggctgtaa
     2761 agctttgttt tcttgtcatt ttgccaatcc cagatctcgc tggtgtatct gtcttgaatg
     2821 tctttataag gaatgcggta gatcctgatc tccagccttg ggatgttttt atagctgacc
     2881 agcactttga cgggagtatt ggggatcacc agatcttccc cactgatgct caactccctg
     2941 ctgttgaggg tgttatacag cgcgcggcat gctctggccc catagctgga cgggaagcgt
     3001 ttcatcgcat cctgacagat ctccgcggcc gttttgaggt cccagcgatg ctcctcagaa
     3061 agctcggtct tgaaagaagc ggccagactt tgatacaaca aagccagccg atacgatgcc
     3121 agagcgctgg caggattggc ggaataatct tgcaagagca ggcgcagagc cttttcatag
     3181 agcgaggcct tgtctgccag agaagattta cgatatacga agctcagcct gtccagattg
     3241 gcttccatgc gagccgcggg atcggaatcc gccagatgga aaccgatcag gtcctggaac
     3301 agattgacgg ccagatagtt gagagacaga gagtctgggc tgtcaatgct catcgcggca
     3361 aaactggagg cggagctgaa gtagccggat ttatcgagtt taaactcatc gggaggacga
     3421 gtgaggccgg attcgtcgtt gacgtaaaaa tccagggccc gccaggccaa aaagtcgtag
     3481 agagtgggcc tcaactgctt ttcatcggct cctcccttgt tgataatggc cggataatcc
     3541 caaatgctga tcgtttgcag gtcctgaggc ctttggagag acaagcggta ggccttgatc
     3601 gtttcttccg ctatggtagc cagatcccag gtggcgatgt catctctttg gaaattggtg
     3661 gtttgggtgc ggttcgagaa gaggtagcgg ttgctctgat aatagctcca ataaagctgg
     3721 gccagcatgg agtgcaggat cgcgctgctg ggaaagcgcg cgctttccag cctcaagcga
     3781 acggcatcga tggctttctg ggctgagaat tcctctttct tttgcaggat cgtgagctta
     3841 tacaccaggc ctttgatctg ctgctccacg attccgtcgc tgtaggcagc cgccaaaaga
     3901 gaatcgatcc tggcatccgc cgaaactggc aaacctttac ccagcagggc atcaatttct
     3961 ttccactgct tgccgtaatc gaagctgatt ccaaaggctg ccgcagtgca aagcagcagc
     4021 gtgaaaacca tgatcagtcg cgccgttggt gctgtcatgc tgtctcctca tggccgtcgg
     4081 aggctgagtt tttgattgat attattgcct cgcacgccat attcgcaaca atactgcgca
     4141 gggatgatac gtcaagcgat aaatctcacc ctggagtgga tcgtcacgcc acccagatcg
     4201 tcccctctat tcttccttat tcacccgccg tggagatacc gtgcgctctc ccgggttttt
     4261 cggcggaggc cgcacggcat ctccacggcg ggtgaccagg caaggatttg ccgtccagca
     4321 agataactgg tgtcttgaga gccttactcc cccagcagct taaagtgttc ccaaatgtgc
     4381 tccggcactc tggcgggttt gccgttatgt atatgcacca catggatttg agcacggaag
     4441 cagagctctc cggcctcgtt gtagatctcc tggttccaac tggagcggag cttgttgcgg
     4501 tcatagatct ggctgcgcac ttcgatgatg tcgtccactc ccacaccttt cagaaattcc
     4561 acctcgattt tgttgatata gacgtgccag ttcagctcca tcaggcgttt tacaggcatg
     4621 ccgacatcca ccagggcttc ggaacgggca gcctctaaga tattgagata gttggtgtta
     4681 tgcaggtggc cgtagaggtc gcactcatag ccatagatgc gtttgcgaaa gttcgctatc
     4741 atgataaatc tcctgataaa tcaagagggg acctccgaat ggggatcccc tctgcgctta
     4801 taccttgtag tttagaatgt tacaggtgtt tttctgtcct cgtgcaaggc cgtcatcctg
     4861 ctttgcagtt cggtcttgat ggctctggga gaggcatcca gcttgtcggg atccacgttc
     4921 ctgaaatcaa agaccttgac aggcttgctc ttcaggccgt cgagcagttc cgtggcgagg
     4981 gtgttccaat ggttgatatc tgccgtggcg ggattgatcc atcccagttc atagagcagt
     5041 ttcatcagtt cgcagatgtg attggctttg gcgggatcgc ggaagaaact cacggccccg
     5101 ttgttgccaa gcgtgctgcc gtcgatttcc agataggatg aagccggcaa cagcagatcc
     5161 gctttggaat catcatcttt atagagctgg atgctggtcc tgaaagcact gctgtcctga
     5221 tccctgagcc cggtgcccca ggcgatcaca aaatcggcag tggaaggcag gttgaagcag
     5281 ggctcaatgg cgatcagccc ccccagattg ggcagatctg agcctgccat cacgttacgg
     5341 ggatagacct tatccagagc ggccagcttc cagacgccgt aagcttctct ggcgctaagc
     5401 tgttcctgat tatagacata caagcggctt gaagcttccg gaaggtcgca tagacgtggg
     5461 tagacggcat ccgcaaacct cagccagggg gcttctgact taccgacgaa gattaaacgc
     5521 ttgccgatcc gctgtttgag tctcaggcgg gtgcgggtga cgaggtcgat ctcgccgcag
     5581 accacatact cctcaaactc atccaatctg gcaaagggga agggatcggg agccacaaaa
     5641 tagctctcgg gccctaaagg cagacagcag ggatcggctt gcagccttgc tccacagctt
     5701 tccgcggcct ttttggcgat gagcagctct tcgatggcga gactggcaga gacgtcgaag
     5761 cgtttcttct gagcggtagt ggccttggag gcgatcagct ctttggcttc ctgccaactg
     5821 atgtctctcc agacgccgtt ttcctttgct ctgggggttc ggagcctgtc ttctgacaag
     5881 aggacctgcc agccaaagcg acccttgaag cagaggtcac gtccgttgaa gccttgcagg
     5941 tcatcctcgg aaggggtttc aatccgggtc acgacgccgc tctggtcttc cactttgata
     6001 tagcagccgg tgccacagag tccgcagttt tgcagggttt cggtcttggg cagcgggttt
     6061 agcttgtagt gcagattgcg ctccaccaaa gctcccaccg ggcacacggc aatgcattta
     6121 ccacagcttt cgcaggtggt ttgagtgagt gattcgccaa attcgggtgc caccacagcg
     6181 gcaaatcctc tatagatata tcccaatacg gcagggcctt gaatctccgc gcaggtgcgc
     6241 acacagcgac cgcagttgat gcatttgttg gcatcgcgaa cgatgtaggg gtggctgtaa
     6301 tcgatgggat gcttgttgat actgcctgga aaggacaggg cgttcacctc gtaatccgtg
     6361 caatactccc tcagcgcgca ggtttcattt acctggcagc cgcactccag acagcggtcc
     6421 gcctcagctc tggcgtcggt ttcggtaaag ccggtctcca cctcgtcgaa gctggtttta
     6481 gcttgcggga tggggatttc cggcatcttg aaacgggcga tcttctcaaa gaggctatat
     6541 tctttggcag aaatctccga gatttttttg ccctttttag aatcgaactt tggccgggcg
     6601 ggtgggaggg ctaaaccatt gagatagcga tgaatggcct cggcggcagc ccgaccgtcc
     6661 gcgatcgctt caatggcagt ggcggctccg cgacggaaat cgccacccgc gaagacgttg
     6721 gccaggcccg tgtgcatact gccttcatcc acgatggcag tctgccagcg gctgataggg
     6781 ataatctgtc cgtctatctt gttgggatcg tctgcaaaga gatcgacctc agggacctga
     6841 gagatggcgg ctatgatgga atcaaactcc tgggtgaaga attcgccagt gggttcggga
     6901 cgtcttctgc cgctggaatc gggatcaccg agcttcatct tttcgatctt tacgctcttt
     6961 agcttgccgt cctcgccaaa gtattccacg gggttgcaga ggtagtgaaa cacggtgcct
     7021 tcatgaccgg cggcctcgat ttcaaagggc tcggcaggca tctcttcacg ggtgcggcga
     7081 tagatcaggg ccacttcaca acccagccgg atagccgttc tggcacagtc gatagcggtg
     7141 tttccacctc ccacgattgc tacctttttg cccaaatggg gtgtgtcgcc cagcgcgtga
     7201 gctttgagga aatcgactcc cagatagcag ccttgcagat cgctgccttt cactggcatg
     7261 ggcaccgctt tctgagctcc cagagccaga tagacggcat cgtaagcggc ggccaggtct
     7321 ttgagactaa ggtctttgcc cagtgccttg ccatattcta tcttcatgcc gttggagcac
     7381 ataagctcga tctcggcatc cagcgtggcc ttgggcaaac ggtattccgg gatgccgtaa
     7441 cgcagccacc cacccgcttg gggagcggct tcaaacacgg tgacggcatg accctgatta
     7501 ctcagataat atccacaggt aaggccagat ggaccggccc cgataatggc gattttcttg
     7561 cctgtatctg cttctttttc aggaatatag ttccacacgt cgccaaggtc ctcatcggcc
     7621 gcgtagcgtt tgagctgacg gatagcgaca ggctcttcca cgatctgacg ccggcattcc
     7681 ttttcacaga aggcggggca gactctgccg atagataaag gcatggggag agtatcttta
     7741 atcactttta cagcttcgtg gtattggccg ttggcgatca gcgacacata gctctggata
     7801 tccacgtgat cagggcaagc gatcttgcag ggagcttcgc aatcggcata gtgattggag
     7861 attagcagtt ccagggccat tttgcgggcg gcatggattt cggggctgtc tgtcacgatc
     7921 tccataccgg ggctcacgag ggtaccgcag gaggtcacaa atccacgtct gcctttcact
     7981 tccaccgcgc agacccagca ggaaccataa ggcttgagtt cttcatcgtg gcagagggtg
     8041 gggatctcac aaccgtgtgc tctggccagg tccagaatac tggtgccggg ctcggtttcc
     8101 actttttgtc cgttcaggat tacttctatc atcgcaggtc tcctatccct tcacaatggc
     8161 gttgaacttg caggttttgt agcaggcgcc gcatttgatg cacttgtcct gatcgatcac
     8221 atgtggctgt ttgacgctgc cggagatgca gttgaccggg cattttcgag cgcaggcggt
     8281 gcagcccaca cacttttccg ggatgatctc atatttgagc agagcggtgc aagctcctgc
     8341 cggacagcgt ttttcctgga tgtgggcgat gtattcatgc ttgaaatagc gcagggtggt
     8401 gagcacggga ttgggggccg tctgtccaag cccgcagagc gagcctttga tgatgttggt
     8461 ggccaggtcc tcgaggcgct cgatatcttg catcactcct ttgccttctg tgatgcgggt
     8521 gaggatttcc agcatcctgc gagtgccgat gcggcagaaa gtgcatttac cgcaggattc
     8581 attctgggta aaattgagga aatagcgtgc cacatccacc atacaggtac cggaatccat
     8641 caccaccata cctccggaac ccataatagc cccggtggca ttgatgctgt cgtaatccac
     8701 tgtggtgtca agcagttcgg cagggatgca accaccgctg ggacccccaa gctggacggc
     8761 cttgaagggt ttctctgtct tcatgccgcc acccactttg tagatgatgt cccggatcgt
     8821 gatgcccatc ggcacttcaa tcagaccgct gcctgcgatc ttgccggcca gggcgaagac
     8881 cttggtgccg ggagatttct ccgtgcccat agcatggaaa gctttggccc cgttcaagat
     8941 aatccaggtg atgttggccc aggtctccac gttgttgata ttggtgggtt ttccccaaag
     9001 tccactctcc gctggaaagg gaggacggat agtgggcatg ccgcgtttgc cttcaatcga
     9061 ttgcatcaga gcggtttcct cgccgcagac gaaggccccg gcgccctctt tgatgtgcag
     9121 ttcaaagctg aaatcactgc ccaggatgtg tttgccgaga tagtttttct gttcggcggc
     9181 ttctatggcc accttgagat gggcaattgc aagagggtat tccgcccggc aatagatata
     9241 accctcgtcg gcacccatgg cgtaagcgcc tatcaccatg ccttctataa tcttatgagg
     9301 atcgccttcc agcacgctgc ggtccatgaa agctcccgga tcgccttcgt cggcattgca
     9361 gaccacatat ttcttgggtc cttgcgcgtt tttggcaaag ctccacttga gtccggtgga
     9421 aaaacccgct ccacctctgc ctctgaggcc ggattcctta acttcggtaa tgatctcgtc
     9481 tccgctcatg gtgagggctt tgcggagggc ctgatagcca ccgctggcct cgtaatcttc
     9541 cagagacttg ggatcgatca cgccacagtt ttgcagcacg atgcgtttct gattggccag
     9601 cagcgcgttg cggggagcgt catatttttg ggccagaatg acatgcgaag atggtacgcc
     9661 gcccgccaga tactcttcca ccaaagtggc gatgttgtca gcagtgactt tgccaagcgt
     9721 gacggaacca gcctctgagc cgctgagctc cacaatcggc tcttcaaaac acattccaat
     9781 gcaggcggtg cgctgtagct tcacaggcct cacgttctcc atcagatagt tttccagctt
     9841 gcgatagacc tcatccgctc cagccgcgat gccgcaactg gccagtccta cttttacagt
     9901 gatcgcttgc attttccctc cctaaaacga gtatttttcc agaatgccgg ggatggcttc
     9961 cggagtaagc tttccgtagg tctgatcgtc caccatgatt acgggggcca gactgcagca
    10021 gcccagacaa gctacttcca tcagggtaaa acgcatatcg gaagtggtgt tttcgccatc
    10081 cggcaacttg agatgttcca ccagggcgtc cttgatcccg tcggcgtcgg aaacatgaca
    10141 ggcggtgcct ttgcagacgc ggatgatgtg tttgccctgg gctttgagcc tgaacatgga
    10201 atagaaggtg gccacaccaa agatctccgc ggcggggata tccatcttgg cggcgatgta
    10261 gcgcatcgtg tccttggaga gaaacccctc cagatcctga acttcctgca tcatagggat
    10321 aaggatgcct ttcttgcccc gatacctttc gagcagggca tccagcttca caaagtcttt
    10381 ttgcatagtt tgatcctttg caatcagata tttatctcac cctcacacac acatacatca
    10441 ggggcaagat cttggtttca caccaaaaat gagtcaaggg gaaatgtcaa acgatttatc
    10501 agccggcaag aaaagaagca tggaatccaa gcaaaacagg ccagcaccaa agcgccggcc
    10561 tgccatgtca ttatgggtta gaacgagtat gatcctcccc catcaatatc catcttaaac
    10621 atacccctga aatgatagcg aggcatgttt tcaccgctgg gattgagggt ccagccttcc
    10681 ggaacggaag aagcgctcca gggctgagga ggagtcttgc caccctgcag aatcgccatc
    10741 gcggctgaga tattggcagg caggacagac aagtccagat cgggtttgat gatctcggca
    10801 gccaaagctt tgctgaagta ggaattggga gcagggctat ccaccaaaac cgtatccaga
    10861 ggagagccat acatcagcag ggtgttgcca ggcagcttgg caagggcagc catgcctggt
    10921 ttggcgagct tgaaaccgat attggcaggg ctgcgggccc catccaggaa tatcagtttt
    10981 actcccgctt tggagagctg ggaaatcgcc caattgagct ccacagcgct ggtcttgaag
    11041 gtggcggcat ccttgaagat gcctaccggg accagatagt tcagaccatc cagttgaatc
    11101 acggccccgc tgaaatagac catggccatt tcaccctcgg cgagagtgtt gttaaactgg
    11161 ctgagagcgg tcttgagggc cagcgggccc agattctgat gattggtcac cgtccagccg
    11221 ctttgggcca gggtgctgtc tatcagagcg gcgtccctgg caggttgcgg caaggctttg
    11281 ctggcatagc cagaattacc gatcaccagt gctttttgag ctgagagcga gaccaaagtc
    11341 aggctcagca gcagcacaag tgtcaaaaat ctcttcatca tatcctccac gatgttatta
    11401 ttggtgttgt ttgggagcgg cggcactcgc tttgatggcc tcgagcacct cagtagtatc
    11461 ctttatacca ttctcatcgc ccaaatactg cttaattctg atgattcgct cataacaagc
    11521 gatggctttg tcatattcct gtaaggccgc gtgcaccctt cccatgttgc ctaatgcttt
    11581 gatagtctcg atcttgtcgt tcatgcggtt agccagttcg agcttctttt caagataagg
    11641 catggcttcg ttcattctgc ccatcacgat attggcgtgg cccagattgc cgatcgccag
    11701 cgattcgtct ttgagatagc gatggttttg ggccagctcc agactctgct gaaaggcctc
    11761 cagagcaggc tcatattcct gcctggccag atgcagcaac ccgatattat tcaaggactt
    11821 ggcctcacgt tggggatctg ccaggctcct ggccagagcg atgctggatt gatgacgctc
    11881 caaagcctgg tcccagagcc cgccatcctg ataccagatg cccatgagat tctctatcat
    11941 acagcgcggc ataagattct ctatcctgcc tgccaattcg gccgcctggt cccagcagac
    12001 tttggcctca tctctctgcc ttttgtagaa agcgatcatg cctctcagac gctgggcatg
    12061 gaaacagagc tcgggatttc ctgctttgag gcccagttcg tttagggcat ccagatcggc
    12121 gccgaggctt ttccaggccc ctgtgagcca tcggatctct atacactgca agaggatgtc
    12181 ccccctttgt tgatagtttt cctgaggcag cagatccaaa agctggcaat agaggtccag
    12241 cgcgcggttg ttgtcaaaga cccgggccgc ggaatctgct gcttttctca gccagggcaa
    12301 ggctttttgt ggcagctccg ctttcagata gtgagttccc aggttaaagc tgaagtcatc
    12361 caggcgatcg ggaaagaggg cctcgatggc ctcggccgtg agctcatgca acaccttgcg
    12421 gttggtgatc aacagcgtct gataggccac ttcccgggtg gtgatgtgct tgaacagata
    12481 gctggaaaac tcgaatccca gcagcttgat gatcaggctg tggtcttcca gagtggccag
    12541 ggtggcatcc acatccaccg ggtcctcaag gttgcgttcc acctctctga ggatctccac
    12601 aaagaattcc tggccgatca ccgcagcctt ttgcagcaac actctcagca cctggggcag
    12661 gctgtccagt ctggaaagaa tgagggcatt gaggctggcc ggaaccggca tatcctgcca
    12721 ctgagtttca cccagactgc tcacatagtt gcaccactct tccagataaa acggatttcc
    12781 cgccgagagg gataccacct gttcgatgct gcgctccgaa aggtccagat gcctcgtatg
    12841 cttcagcacc agctccgtga tggatttttc gtccagaggt tccaagctga gttcattgaa
    12901 accacgcatc ctggccagat aggagggcgg atcgaattcg ggccggtagg gcagcagcca
    12961 cagactgggc aacttgctct gggagaagcg gttcaccaga tgctccagaa ccgccgaaga
    13021 gctgtcgtcc agccagtgca gatcgtcgag caccagcacc aaaggctttc ccatttgggc
    13081 gcattggacc agcacggctt ccaaaactgt gttgacggcc agcagcagat gctggagcaa
    13141 gtctttaccg ccttgtttga gccggggatc agccactctg atttccaaaa gccaggcgat
    13201 cagaggaaag gcgtcctgga ggctggagcg cagttccact tcggccgtgg cttccagagc
    13261 ggaaaacgcg gcctggagtt tggcgagctt tgcatccacc ggctccgtgg tccgcagacc
    13321 aaaacgctgt tccagcagac gggcaaagag atgcagcggg gtgggactca ctccgtcaca
    13381 gccagccacg atgaattccg ccttatcgct gttggagcgc cgaaactcat agaccagcct
    13441 gcttttaccg attccagcgt cggctctgat gccgatgatc actggtctgg gagctggcag
    13501 ttccgcttgg ggagatatct tcccagtctg ggcaataatg aggtccaaag cttcttccaa
    13561 agctctgagc tcggtttccc tgccgataaa actactgcca tagcgctccg ccagactggc
    13621 ttccaacgtc ttgagcccca aaggactcca gcagtccacc gctttgtcta tccccttcac
    13681 ctgtttgagg cccaggggtt caaactcgaa tatctgctcc accaactttt tggtggtatc
    13741 cggaaccata attctgccca cgggcgcgtt gctttccatg cgggaggcga gattgacctc
    13801 gggaccgtaa acggtgaaat ctccctcgcg tgattcgccc acgcggccta cactcacaag
    13861 gccggtattg atgccaatcc tcacgctcag ctcaacgtct tcatagccag gcatttgcct
    13921 cagattatga ttgtagagct gcagttgctc caacattttc aagccggccc ggatggcacg
    13981 ttcggtatcc tgttcgctgg cttttttggc cccaaacaag gccatgatgg catcgccgat
    14041 atatttatcc acaaagccgc cgtaaaaggt gacgcagcgg gaaaatactt tcaagagctc
    14101 gtccatacgg gcatggatga cctccggatc gagacggctg gagagattgg tgaagccttt
    14161 gatatcggca aagagcactg tcacttcacg cagttctccc tcccgaagct ggagtttccg
    14221 cttctgggtg aggttgtctt cttccaggcc aaaaaagccg gggccaaaga tcatgtcgtc
    14281 tgtcaattcg gctcctgatt tgagtttccc attagtgaag atggcgttgg tgacaccttt
    14341 ttacaaagtg gggatgggag tcaagggagt tttttctgta gcgtcggatt tctatctgct
    14401 gccatacgga accggaaacg tcctcgtttc cggaggctgg acatagttta gccaatttgc
    14461 tgccaaaacg caggtctggc ttgccacgcc gcaggcgctg atcgcgcctt ggcaaacgag
    14521 gacgtttgcc gctccataat cgtgcccatt tgttgccgta gagatgccgt gcgctctccg
    14581 ccgaaaatcc cgggaaagcg cccgggatct ctacggcggg tgattaagca agcaagccgg
    14641 tagaacctca aaggtccgtt tttgcatccc cacagacaca tcccctgatg gtatgccagt
    14701 atctaaagag aggcagttcc cacccacttc ggacaaatgg cttttgggtg ggggatgctc
    14761 tgggtattgt cccctgcagt cagcaagaga cgtcagggtg tgagtcttga caggaatcta
    14821 aacgcgggat tcctataaac gagaacgggg tttctaacga gctcagaagc cattgtgacg
    14881 agcgtaaatc catttgtatc actggcttca g
//
LOCUS       DAOSFN010000052        14786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3327_length_14786_cov_7.977870, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000052 DAOSFN010000000
VERSION     DAOSFN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 14786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14786
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_14786_cov_7.977870"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2859
                     /locus_tag="PLO35_04820"
     CDS             <1..2859
                     /locus_tag="PLO35_04820"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79023.1"
                     /translation="MQIPMRLRMYNAQGFEQFSTFNLPIGTVSQTTPLGPDEYGYLIY
                     DVTDTGYPDCPSYSWDEISPTLGGSGSAITGFNDPSDGYDEGDIIGAVSTQVVPLPFT
                     FGFYGVDYEQITVCSNGFVAMGVTEDGDYRNVHIPGGQGPSPMIAAFWDDLCMAAGSA
                     IYKYHNAMEHTFVIQYQGLKNGYNGSSEETFQLIFYDPLYYPTGMGDGAIKIQYKVFN
                     NIDIGSNSGYTPLHGNYATIGIKDHTNTRGLEYSFNNQYPSAAAPLSNQKALFITTVP
                     VIHQNPYLVMGETYLYDSNQNGVVQPGETCELGLKLTNIGLDNATMVSVSISTLNPNV
                     TVTSGQSTYPDIASSQSQVNVLPFQFYVSPDCLDGSIVNFNCRVEIDGNSWNYPWTCT
                     VKKAHLELTSMFINDSQGNGNGILESGESFFLILNFQNDTPVDASNLISNIGCVSEHV
                     QILNPMVQIDTVPAGGFGQAVYPVALSDTTPLGTFLTFFVTFAGDEIASQNEQLIVSV
                     GTTGLNADFEYSDGAFIASPVTNGWQWGASNPAGAHSGTKVWGTRLNELYPNNVNWTL
                     TSPAVQLGTNYVLEFWHRYAMEATYDGGNVKISVNNGASWSLLTPEGGYNHSSVAALN
                     GPGYSGTQDWTEARYNLGAYANQQVRFRWTFASDTMIQGDGWFIDDVRTSGAMEYAGM
                     LSGTVTSDNPSAELDQVEITSSLGVGTKALPDGAYQLFLPLGTHTVQASQPGYQSETA
                     DNLILTPANSTVIRDFMLPYYAPVTGLSHQILEDQLSLSWVAPTQQSLTCTGFKVMRR
                     FNAGAFECVSTQTQTAYSETIISDGSYRYYVLATYDTGVSLASETWSFEYPVTPNPED
                     DLPQLVTGLYSNYPNPFNPSTTIRFSLAQAMKASLRIYNSRGQLVNTLLDAALPAGQH
                     SLVWNGKDGFGRDVASGVYFYRLSAGSRVMHRKMLLLK"
     gene            complement(2914..3393)
                     /locus_tag="PLO35_04825"
     CDS             complement(2914..3393)
                     /locus_tag="PLO35_04825"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOH79024.1"
                     /translation="MIELPSTDLHVVLYQPEIPANTGNIGRLCVGTGATLHLIKPFRF
                     LLTDRELKRAGLDYWPRLKIVTHDSLDEIFHTFPMRRIFLCTTKTAQSYAERSYREGD
                     VFVFGPETRGLPPELLERYPDQCIGIPMTAEIRSHNLANSVAIILYEAVRQIIGDKS"
     gene            complement(3444..4550)
                     /locus_tag="PLO35_04830"
     CDS             complement(3444..4550)
                     /locus_tag="PLO35_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH79025.1"
                     /translation="MIKVIAQKRLTALLLTMLLGLIILSGCGRKETKTKTQPGAKSQN
                     PPSEMSVWGLTRLRASGLDQTLFAEFSKKYNCKINLQLFETLPDLLDSLAHIDSTQTG
                     SCDLIMNLDSSFSSNEETRALFSPLPEISTLQLSRDLILDPQKRLIPYGYANLGVVFN
                     TRQFPKGPESFGELQDARYFRQLGICDPKTSGVGRSTLYWSLALFGARGYEYLWKSVR
                     KNVQEVYSDYESGLEALKNGKISMLIGYNTTPAWLEESQQTDKSFQYSMLKEGAWQYS
                     ELAGVPLRSTRPAMAAAFIKYLTEPEAQLMIIYKLGLFPANSKTMLPIRFARIPISSF
                     VVNRRLTEAQIQAGLPVWLEFWDELFSNRYPAFD"
     gene            complement(4547..5296)
                     /locus_tag="PLO35_04835"
     CDS             complement(4547..5296)
                     /locus_tag="PLO35_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH79026.1"
                     /translation="MSYRFLAGLGDYVIFTWAALKSVRSAPRRRQEILLQLKRIGFDS
                     LFLISLTAAFTGLVTALQAVYQSKGYIPINLLSVLIGKSTMVELAPVLTALVMTGKVG
                     ASIAAEIGTMKVSEQLDALRSMNISPAGFLFMPRILAGLITFPMLTVFADLVSIICAW
                     YFSWVRYGIHWHTFFNNMKSYFEPFDLWSGLIKSAVFGFLITTISCYFGDRSSGGAEG
                     VGKSTTLTVVYSSVTILIMDFIVAWILFGAM"
     gene            complement(5286..6659)
                     /gene="dnaB"
                     /locus_tag="PLO35_04840"
     CDS             complement(5286..6659)
                     /gene="dnaB"
                     /locus_tag="PLO35_04840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOH79027.1"
                     /translation="MAKKPDATEPAKISERGLPADVNAEAAVLSAMLIDSDVVSKGIE
                     MVKEEYLYKTAHKLIFRTIGELFNESVEIDVLTLIDRLQRNNLLEKVGGTPYLSELVD
                     YVVSSANFEYHLNIVVEKALLRHLILACNGIIESCYSSAKPVKTVVDEAEQAIFAIAE
                     LPTHQGFIKLDQISAEVLETIDTIASTKIPVVGVPSGFSDLDRLTGGFRPGQFIIIAA
                     RPAMGKTSLALNIATHAAVDLGKKVAIFTMEMAADEVVMRMFSSAAEVNMESMLKGYG
                     MNEEKLIRIMQASDVLSTRHIYIDESGTNTPLDIRAKTRRLAAEVGGLDLILIDYLQL
                     MTLPRDRENRQQEISEISRALKILAKDMKIPVVALSQLNRMLENREDKRPRLADLRES
                     GAIEQDADLVMFIYRDEYYYQDKSEKPGIAEIIVGKNRHGSTGAVDLGFVKEFTMFRN
                     VDTTHEL"
     gene            complement(6669..7151)
                     /gene="coaD"
                     /locus_tag="PLO35_04845"
     CDS             complement(6669..7151)
                     /gene="coaD"
                     /locus_tag="PLO35_04845"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOH79028.1"
                     /translation="MHRALYPGTFDPITLGHLNILEKAVRLFDEVILAVADYTGKNTS
                     IQLEERLRLCVESTRHIPNLKVLSFSGLVVELAEKLECNTMLRGLRAVSDFEYELSLA
                     LTNKKIKPQIETVFLVPSLKYMYLSSSMVRQMADLGAHLEDFVPEPVLQYFHDTKRAR
                     "
     gene            complement(7154..8383)
                     /locus_tag="PLO35_04850"
     CDS             complement(7154..8383)
                     /locus_tag="PLO35_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOH79029.1"
                     /translation="MSADLYIARREKLADSLQDGENCIVFAKSAENLAKFTQDNNFLY
                     LTGLNHPELIYVAGKFGAKFNEMLFIERSDPERVVWEGAKLGVEEAMQISGIKRVRYL
                     DEFYPVLSGMCPMMKVIHANIGFVALNRPLSLPMHMLEPIRTRHPQIQIRQLNDLIAP
                     LRKIKDDWELTQLQKAIDLTGKGIVDVMEQARSMMYEYELESILFYRLQRSGVRNWGF
                     APIIAAGVNAATLHYEKNECQIENGQLVLMDVGASYNNYSADITRCFPIGERFSERQK
                     QIYQIVLDAQKEIISMIKPGVKLSELNIRTRELLTESAIAIGLISDPDDIIKFYMHSV
                     SHFLGMDTHDLGGREAVLEVGNVITVEPGLYLAEEKLGVRIEDDVLVTENGHCVLSQN
                     IPKEIDEIEEIRRMALS"
     gene            complement(8508..9110)
                     /locus_tag="PLO35_04855"
     CDS             complement(8508..9110)
                     /locus_tag="PLO35_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79030.1"
                     /translation="MRKMMILGLLVLMGLLLMACEEDGELRIRNRTNAMVQARVDDST
                     PMDIDAWSGWSRYYTEDTTVKVSFEGLYVYPDSTTRSVIVGLPTTVNILPDAGALKIV
                     NDSTFAITEIYISAHDATEWGENQIADSLSVDAERIWTTDGGAWDIKIVSTQGTPLYK
                     MNQTVTINETLALDVSDFLPFAGLKKTGTQIRTTRRPIAR"
     gene            complement(9122..10366)
                     /gene="clpX"
                     /locus_tag="PLO35_04860"
     CDS             complement(9122..10366)
                     /gene="clpX"
                     /locus_tag="PLO35_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOH79031.1"
                     /translation="MDKYKNLSCSFCGRAETEVKTLIRGIAGNICDECIGMCHNIMET
                     SEPPATDQEFELPIPSRIKQYLDQYVIGQENAKMIISVAVYNHYKRIFRQSARDEIEL
                     EKSNILMIGPTGSGKTLIAQTLAHFLKVPFAISDATTLTEAGYVGEDVENILVRLLQA
                     ADYDVAKAERGIVYIDELDKISRKSETPSITRDVSGEGVQQALLKILEGTKVNVPPKG
                     GRKHPQQDFIEMDTKNILFIAGGAFFGLEKIIKERMDKKVIGFDVKLAEKTDETNIFE
                     HVGSSDLLKYGLIPELIGRMPVTCTLHELSEAALMDILTKPKNAICKQYEKYFQLDGV
                     VLHWEPEALKEIARIAIKQQTGARGLRSIMEKFMLRIMYEIPDRPSLKTCTITAESVK
                     RLKEPEYTETEALSTRGKTAVK"
     gene            complement(10495..11079)
                     /gene="clpP"
                     /locus_tag="PLO35_04865"
     CDS             complement(10495..11079)
                     /gene="clpP"
                     /locus_tag="PLO35_04865"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOH79032.1"
                     /translation="MNYIPMVIEQVGRVERAYDIYSRLLKDRIVFVSGVIEDNLANSI
                     VAQLLHLEGEDAERDIYMYINSPGGIISSGLAIYDTMQYIKPKVSTICIGQAASMAAM
                     LLAAGAPGKRTALPYSRVMIHQPLGGAQGQASDIEIQAKEILYLRRVMNELMQKHTGQ
                     PLERIEADTDRNFFMSADEAKAYGLIDEVISQRK"
     gene            complement(11076..>12384)
                     /locus_tag="PLO35_04870"
     CDS             complement(11076..>12384)
                     /locus_tag="PLO35_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOH79033.1"
                     /translation="FKENKVQTDIKQLDPVTKSITITIDAETAARDYQKFLSRSARDI
                     QIPGFRKGKAPLPMVERMYGETVKDYFLKDATDDYFNEVIKEHQLGYLLMPHVKDIQW
                     EKGSEFKAEIEIEHEPEVELSIPDPLVVPHEAISLDDEVERFLKKMAQENATYLQVEE
                     VSADDAVECEIKIAVNDETLTHTGVLFAGDAMPQRSVAELVGKKVGDVVETQLSGNSL
                     KLVTRTSIDKLDNDTSYPCSLMVNDIRRYVEPAIDDEFAKDMDHEDLASLKAEIASNM
                     KLRLEHNNFDAENNAIIHKFYADGPFDLPKRTIEYLAREEAAKIEDKRYQQYYEYQFR
                     MQFSQQMINVYIMKALNRLLNPTLDDAMLDQYIEHEAILEDIPVGAWKEKHAERLSTE
                     DFKDEARSWTMLRNLAARAEFVKPEPPAVEPNEENPEVNEENA"
     assembly_gap    12385..12484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12653..12738)
                     /locus_tag="PLO35_04875"
     tRNA            complement(12653..12738)
                     /locus_tag="PLO35_04875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12702..12704),aa:Leu,seq:tag)
     gene            complement(12811..13650)
                     /gene="panC"
                     /locus_tag="PLO35_04880"
     CDS             complement(12811..13650)
                     /gene="panC"
                     /locus_tag="PLO35_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOH79034.1"
                     /translation="MRVINSITEMKAVCQSLKREGSLGFVPTMGYLHEGHLSLVDQSV
                     SANRHTVVSIFVNPAQFGRNEDLSTYPRDLDRDLELLQSRGTDYVFYPDAAEIYPSEH
                     QTWVEVEKLSVPLCGKSRPGHFRGVATIVLKLVNIVQPDYMYMGSKDFQQVVVLERML
                     ADLNHDTVITRCPIIRETDGLAMSSRNSYLDADERIRARCLSQALITARHSFALGERG
                     TAVLIAEASRLIETAGANIDYVECVDPKLLTPVETAEADTRMVMAVFIGRTRLIDNGA
                     LGS"
     gene            complement(13651..14001)
                     /locus_tag="PLO35_04885"
     CDS             complement(13651..14001)
                     /locus_tag="PLO35_04885"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOH79035.1"
                     /translation="MLRYILLSKLHRGTVTECDLNYNGSIKIDPDLLDAAGMRPFEKV
                     EIFNVNNGARFSTYIIEGERGRGEIAINGAAARMAMVGDKVIIINYGLMDDKEIEGHQ
                     PRIVILDDHNQPVR"
BASE COUNT         3254 a         3901 c         3745 g         3786 t
ORIGIN      
        1 atgcagatcc ccatgcgcct gagaatgtat aacgcgcaag gttttgagca gttcagtact
       61 ttcaacctgc ccatcggaac ggtatctcag accactccgc tgggtcctga cgaatatggc
      121 tatctgatct atgacgtcac cgataccggc tatccggatt gcccttctta cagctgggat
      181 gagatcagcc ccacgctggg aggcagcgga tccgccatta ctgggttcaa tgatccgtcc
      241 gatggttatg acgagggaga tattattggg gcggtatcaa ctcaggtagt tccattgcct
      301 ttcaccttcg ggttttacgg ggtcgattat gagcagatca ctgtctgcag caacggattc
      361 gtagccatgg gtgtcaccga ggacggcgat tatcgcaacg tccatatacc aggtggacaa
      421 ggccccagcc caatgatagc tgccttttgg gacgatctgt gcatggcggc tggatcagct
      481 atctataagt atcacaacgc tatggaacat acctttgtca tccagtatca gggcttgaaa
      541 aacggttata acggcagctc cgaagagacc ttccaattga tcttctatga tcccctctac
      601 tatcccaccg gaatgggtga cggcgcgata aaaattcagt ataaagtatt caacaacatc
      661 gatataggca gcaactcagg ttacacaccc ctgcatggaa actatgccac cattggcatc
      721 aaggaccata ccaacacccg cggtctggag tatagtttca acaaccaata tccctcagcg
      781 gcggcccccc tctccaatca gaaagcgttg tttatcacta cagtcccggt cattcatcaa
      841 aacccctatc tggtgatggg ggagacctat ctctatgatt ccaatcaaaa cggagttgtg
      901 cagcctggcg agacttgcga actgggcctt aagctcacca atatcggttt ggataacgcc
      961 accatggtgt ccgtcagcat cagtactcta aaccccaatg tcacggttac ttccggtcag
     1021 agcacttatc ccgatatcgc ttccagtcaa tcgcaagtga atgtgttgcc cttccagttt
     1081 tacgtatccc ccgattgtct ggatggcagc atcgtaaact tcaattgcag ggtggaaata
     1141 gatggcaact cctggaatta tccctggacc tgtaccgtca agaaggccca tctggagcta
     1201 acctccatgt ttatcaacga ttctcaggga aacggcaacg ggatccttga atcgggagaa
     1261 agcttcttcc tgatcctgaa tttccagaac gacacgcccg tggacgcatc taacctgatc
     1321 agcaacattg gctgtgtgag tgagcacgtt cagatcttga accccatggt ccagatagat
     1381 actgtgcccg cgggaggttt tggacaagcc gtgtatccag tggcgttgag tgacaccact
     1441 cccttgggta ctttcctcac attctttgtt actttcgcgg gagacgagat cgcctcgcag
     1501 aacgagcagc tgatcgtgag tgtgggcacc acaggcctca atgccgattt tgaatacagc
     1561 gacggtgctt tcatcgcctc tcctgtcacc aacggatggc aatggggggc ttctaacccg
     1621 gctggagcgc attcgggtac caaagtatgg ggcacacgtc tgaatgaact atatcccaac
     1681 aacgtcaact ggacgctcac gtctcctgcc gtccagcttg gtacaaacta cgttctggaa
     1741 ttctggcacc gctacgccat ggaggctacc tacgatggcg ggaatgtcaa aatatctgtc
     1801 aacaacgggg cttcatggtc gctcctgact cctgagggcg gttataatca cagttcagtt
     1861 gccgctctga acgggccagg ttactccggc acccaggatt ggactgaggc gcgatacaat
     1921 ctcggcgctt atgccaatca acaggtcaga ttccgctgga cctttgccag cgacaccatg
     1981 atccagggag acggctggtt tatcgatgac gtaaggacta gcggggcgat ggaatacgcc
     2041 ggtatgctgt ctggcaccgt gaccagtgac aatccgtccg cggagttgga ccaggtggaa
     2101 atcacaagct ctttgggcgt aggcacaaaa gccttgccgg acggggctta tcagctattc
     2161 ttgcccctgg ggacccacac cgtgcaggcg tcccagcctg gctatcaaag cgagactgcc
     2221 gataacctca tcctgacgcc tgccaattcc actgtgatca gggatttcat gctgccgtat
     2281 tacgcgccgg tgaccggtct gagccatcag atactggaag accagcttag cctgagttgg
     2341 gtcgctccta cgcagcaaag cctgacctgc actggtttca aagtgatgag gcgtttcaac
     2401 gcgggagctt tcgagtgcgt cagcactcag acccaaactg cttactccga gaccatcatc
     2461 agtgacggca gctatcgcta ttacgtgctg gccacctatg ataccggtgt tagtctggcg
     2521 tctgaaacct ggagcttcga gtatcccgtg acgccaaatc cggaagacga tcttcctcaa
     2581 ttggtgacgg gcttgtactc caactatccc aatcccttca atcccagcac gacgatcagg
     2641 ttctctctag cccaggcaat gaaggccagc ctcaggatct acaattccag aggccagttg
     2701 gtgaacacac tgctcgatgc agccctccca gcgggtcaac actccctcgt ctggaacggg
     2761 aaagatggct tcggcagaga tgtggcttcc ggggtttact tttacaggtt aagcgccggg
     2821 agcagggtca tgcaccgcaa gatgttgctg ctcaaataac tagtccatcc ttattgtgcg
     2881 aggcgggacg gctttgtaaa agctgagccc gcctcagctt ttatcgccaa taatctggcg
     2941 aactgcctcg tagagaatta tcgccacgga gttggccaga ttgtggctgc gtatttcagc
     3001 cgtcatggga atcccgatac actgatcggg ataccgctca agcagttctg ggggcaatcc
     3061 tctggtctcg ggtccaaaca caaagacgtc gccctcgcga taggagcgtt cagcgtagct
     3121 ctgagcggtt ttggtggtgc atagaaaaat ccgtcgcatt ggaaaggtgt ggaaaatttc
     3181 atccaggcta tcgtgcgtaa cgatcttgag cctgggccaa tagtccaggc cggcccgttt
     3241 gagctcgcgg tctgtgagca ggaagcggaa aggtttgatc agatgcagcg tcgcgccggt
     3301 ccctacacag agtctgccga tgtttccagt attggcaggg atttccggct ggtatagcac
     3361 cacgtgcaag tctgtggagg gtagttcaat catagttgat cacatcagcc cgggcaggta
     3421 tcatcaattc ccgggactga ggttcaatca aaggctgggt agcgattgct gaaaagctca
     3481 tcccaaaact ccagccagac tggtagccct gcttgaatct gagcttcagt aagccgccgg
     3541 tttacaacaa acgaactgat gggaatcctg gcaaagcgga taggaagcat agttttggaa
     3601 ttggcaggaa acagacctag cttatagatg atcattaatt gggcttctgg ctcagtcaga
     3661 tatttgataa aagccgccgc catcgcggga cgcgtagatc tgaggggcac gccagccagt
     3721 tcgctgtact gccaggcccc ttccttcaac atgctatatt ggaagctttt atccgtctgc
     3781 tggctttctt ccagccaggc tggcgtggta ttataaccga tcagcatact gattttgccg
     3841 tttttaaggg cttccaaccc ggattcatag tctgaataaa cttcctggac gttttttcta
     3901 acgcttttcc agagatactc gtagccccgt gccccaaaca aggccaggct ccaatacagg
     3961 gtcgatctgc ccaccccact ggtcttggga tcgcagattc ccaactgcct gaagtatctg
     4021 gcatcctgca attctccgaa agactccggt cctttgggaa actggcgggt gttgaataca
     4081 acccccaaat tggcataacc ataggggatc aaccgtttct gcgggtcgag tatcagatcc
     4141 ctgcttaatt gcaacgtact gatttcagga aggggggaga agagggccct ggtctcctca
     4201 ttggagctaa agctgctgtc cagattcatg atcagatcac agctccccgt ttgggtgctg
     4261 tcgatgtgtg caaggctgtc gagcaggtca ggaagagttt caaacaattg cagattgatc
     4321 ttacaattgt atttcttgga gaattccgcg aacagcgtct gatccagacc actggcgcgg
     4381 agccgtgtga gcccccaaac actcatctcg gagggcggat tttgggattt tgcccccggt
     4441 tgcgtcttag ttttggtttc cttcctgccg catcctgaca ggattatcag gcccagcagc
     4501 atcgttagca gcagagctgt cagtcttttc tgtgcaatca ctttgatcac atcgctccaa
     4561 acaggatcca ggctacgata aagtccatga tcaaaatggt taccgagcta tataccaccg
     4621 tcagagtagt ggatttgccc acaccttccg ctccacctga agaacgatcc ccaaaatagc
     4681 aactgatagt agtgatcaga aagccgaata cggctgattt gatcaaaccg ctccagaggt
     4741 cgaagggctc aaaatagctc ttcatattgt taaaaaaagt gtgccagtga atgccgtagc
     4801 gcacccagga aaaataccag gcgcagatga tgctcaccag gtccgcgaag acggtgagca
     4861 tcggaaatgt gatcaatcct gccagaattc tgggcataaa gagaaacccg gccggtgaga
     4921 tgttcatgct gcgcagagca tccagctgct cgctcacttt catcgttcca atttcagcgg
     4981 cgatggaagc tcccaccttg cctgtcatga ccagagcagt gagcacgggg gccaattcta
     5041 ccatggtgga tttcccgatc aacacgctca gaaggttgat gggaatatat cctttggact
     5101 gatacacggc ttgcaaggct gtcaccaaac cagtgaaggc agccgtcaga gagatcagaa
     5161 acagagaatc aaagccgatc cgcttgagct gcaagagaat ttcctggcgg cggcgtgggg
     5221 ccgatctgac gcttttcaga gcggcccagg tgaagatcac atagtcaccc aggccggcca
     5281 gaaatctata gctcatgagt ggtgtcaaca ttgcggaaca tggtgaattc tttcacaaac
     5341 cccaagtcca ctgctccggt cgaaccgtgc cggttttttc ctactatgat ctccgcgatt
     5401 ccaggctttt cgcttttatc ctgataatag tactcgtccc gatagataaa catcaccagg
     5461 tcggcatcct gctcaatggc accggattct cgcaagtcag ccagtcgggg gcgtttatcc
     5521 tcgcggtttt ccaacatgcg gttgagctga gaaagtgcca ccacggggat tttcatgtcc
     5581 ttggccagaa tcttgagggc acgtgatatc tcagaaatct cttgctggcg gttttcccga
     5641 tcgcggggaa gggtcatcag ctgcaggtag tcgatcaaaa tgaggtccag tcctcccact
     5701 tcggcagcca ggcgtctggt tttagcccga atgtccagag gagtattggt ccccgattcg
     5761 tcaatataga tatgccgggt ggataaaacg tcggaggcct gcatgatcct gataagcttt
     5821 tcttcgttca ttccatagcc cttgagcatg ctttccatat tcacttcggc ggcggaagaa
     5881 aacatcctca taaccacttc atcggcagcc atttccatgg tgaagatcgc tactttcttg
     5941 cccagatcga cagccgcgtg ggttgctatg ttcaatgcca gtgaagtttt acccatagcg
     6001 ggacgggcgg caatgatgat gaactgacct ggcctgaatc ctcccgtgag ccggtccaga
     6061 tcagagaagc cggacgggac gcccaccact gggattttgg tggaagcgat cgtgtcgatg
     6121 gtttcaagca cttccgcgct gatctggtcc agcttgataa aaccctgatg ggtggggagt
     6181 tctgctatgg caaagatcgc ctgctctgct tcatcaacca cggtcttgac cggtttggct
     6241 gagctgtaac agctctcgat gatgccgtta caggccagga tcaaatgcct caacagggct
     6301 ttctcaacca cgatgttgag gtgatattcg aagtttgcgc tggaaaccac atagtcaacc
     6361 agttcgctca gatagggagt gccgcccacc ttttccagca ggttgttgcg ctgcagacga
     6421 tctatcaggg tgaggacatc tatctccacg ctctcgttga agagctcgcc gatggtgcga
     6481 aagatcagct tgtgagcagt cttgtagagg tattcctctt tcaccatctc gatccccttg
     6541 ctcacgacat cggaatcgat cagcatggcg gaaagaacgg cggcttcagc gtttacgtcc
     6601 gctggaaggc cgcgttccga gattttggct ggttcggtgg cgtcaggttt ttttgccatt
     6661 atggtttcct atctggctct cttggtgtca tgaaagtact gcagcaccgg ttctggcaca
     6721 aaatcttcca ggtgagctcc caggtctgcc atctgcctta ccatcgatga tgagaggtac
     6781 atgtatttca gactgggtac caggaatacc gtctcgatct ggggtttgat ttttttgttg
     6841 gtcagggcta gcgacagctc atattcgaag tccgaaaccg ctctcaggcc ccgcagcatg
     6901 gtgttgcatt ccagcttttc ggccaactcc actaccaggc ctgaaaaaga tagcactttc
     6961 aggttgggga tatggcgggt cgattccaca cacagccgca ggcgttcttc caactggatg
     7021 gaagtatttt tgccagtata gtccgccacg gccaggatca cttcatcaaa aagccggact
     7081 gctttttcga gaatgttcag atggcccaga gtgatcggat cgaaggtccc cggataaaga
     7141 gctctgtgca tgatcagctc agcgccatcc ggcgaatctc ttctatctca tcgatctcct
     7201 tgggaatatt ctgagacagc acgcagtggc cgttctctgt gaccaggacg tcgtcctcga
     7261 tcctcacgcc cagcttttct tctgccagat agagtcccgg ttccacggtg atcacgttgc
     7321 ccacttccaa aaccgcctct ctgcctccca gatcatgggt gtccataccc aggaagtgac
     7381 tcacgctgtg catgtaaaac ttgattatat cgtccgggtc cgagatcaga cctattgcga
     7441 tggccgattc ggtgagcagc tcgcgggtgc ggatgttcaa ctcgctgagt ttaacaccgg
     7501 gtttgatcat ggagatgatc tctttctgcg cgtccagcac gatctgatat atctgcttct
     7561 ggcgctcgga aaatctctcc ccgatcggaa aacacctggt gatatcagca ctgtagttgt
     7621 tgtaggaagc gccgacatcc attaaaacca actggccatt ctcgatctga cactcgttct
     7681 tttcatagtg cagcgtggcg gcattcaccc cagccgcgat gatgggagca aatccccagt
     7741 ttcttactcc gctgcgctgc agacggtaga atagaattga ctccagctca tattcataca
     7801 tcatgctgcg ggcctgttcc attacatcaa caatgccttt tccggtgaga tcgatggctt
     7861 tctgaagctg ggtcagttcc caatcgtcct tgatctttct gaggggagcg atcaggtcat
     7921 tgagctgtcg gatctggatt tgggggtgcc tggtcctgat cggttcaagc atatgcatcg
     7981 gcaggctgag gggtctgttt aaagctacaa aaccaatgtt ggcatggatc actttcatca
     8041 tagggcacat accagacagc acgggataaa actcgtccag atagcgcact cgcttgatgc
     8101 cgctgatctg catggcttcc tccacaccga gcttggcacc ttcccaaaca acccgttcag
     8161 gatcactgcg ctcgatgaag agcatttcat taaactttgc tccaaacttc ccagccacat
     8221 aaatcagctc cggatggttc aggccggtca aataaaggaa gttgttgtcc tgggtgaatt
     8281 tggccaggtt ctcggcagac ttggcaaaaa cgatgcagtt ttctccatct tgcaggctgt
     8341 ctgccagttt ttcacggcgt gcgatataca aatctgcgct cataactgtg ctccattgga
     8401 tagaaatatg tgaatcgatc tgatgcggtc gctctgaatc tttctaaatg gctgcgccgc
     8461 actcaaaaat caagcgggga tccctttgcc gggatcccca caatagttta ccgggcaata
     8521 ggtcttcgcg tggtcctgat ttgggttccc gttttcttca atcccgcaaa gggcagaaag
     8581 tcgctcacat cgagggcaag ggtttcgttg atcgtcacgg tttgattcat cttgtagaga
     8641 ggagttcctt gcgtgctcac gatcttgata tcccaagcgc cgccgtccgt ggtccaaatc
     8701 ctttcagcat ccacgctgag gctgtcggcg atctggtttt caccccattc agtggcatca
     8761 tgggctgaga tgtagatctc cgtgatcgcg aatgtagaat cattcacaat cttcaaagct
     8821 ccggcatccg gaaggatatt gacggtagtg ggcaaaccca caattaccga acgggtagtg
     8881 gaatccggat agacgtacaa accttcaaag cttactttta cggtggtgtc ttcagtatag
     8941 taacggctcc aaccgctcca ggcatcgata tccatgggcg tggagtcatc cacgcgggcc
     9001 tggaccatcg cgttggtgcg gtttctgatt ctcagttcac cgtcttcctc gcaagccatc
     9061 aacagcagcc ccatcaggac cagaagtccc aaaatcatca ttttacgcat ataaatctcc
     9121 tttattttac cgcggtcttg ccccgggtgg acaaggcctc ggtctcagtg tattcaggtt
     9181 ctttcagccg tttgacgctt tcggccgtaa tggtgcaggt tttgaggcta ggccggtccg
     9241 gaatctcgta catgatccgc agcatgaatt tttccatgat gctgcgcagt ccccgagccc
     9301 cggtctgttg cttgatggct attctggcaa tctctttgag ggcctcaggt tcccaatgca
     9361 gcaccactcc gtccaactgg aagtatttct catattgctt gcagatggcg ttttttggct
     9421 tggtgaggat gtccatcaaa gcggcttcgc taagctcgtg cagagtgcag gtaaccggca
     9481 ttctgccaat cagttccggg atgaggccat actttagcag gtcagaagag cccacatgct
     9541 caaagatgtt ggtttcgtcg gtcttctcag ccagcttgac gtcaaagcca atcactttct
     9601 tgtccatgcg ctccttgatg atcttttcca gaccaaagaa agctccaccg gctatgaaga
     9661 ggatgttttt ggtatccatc tcgataaagt cctgctgagg atgtttacgt ccgcctttgg
     9721 gaggcacgtt aaccttggtg ccttccaata ttttcaacag ggcctgctga actccttcgc
     9781 cggaaacgtc acgagtgatg gaaggagttt cggatttgcg gctgatcttg tccaattcgt
     9841 cgatatagac gatgcctcgt tccgctttgg ccacatcgta atccgctgct tgcagcagtc
     9901 ttaccaggat gttttccaca tcttcaccca cataacctgc ttccgtgagc gtggtggcat
     9961 cggagatggc gaagggtact ttcagaaagt gggccagggt ctgcgcgatc aaagttttgc
    10021 cgctgccagt gggccctatc atcaggatat tgcttttctc aagctcaatc tcgtctctgg
    10081 cgctttgcct gaagatgcgt ttataatggt tgtagaccgc caccgagatg atcatcttgg
    10141 cattttcctg cccgattacg tattggtcca gatactgttt gattctactt gggatgggga
    10201 gctcaaattc ctggtcggtt gcagggggct cgctggtttc catgatgttg tggcacatgc
    10261 cgatacattc atcacagatg ttgcccgcga ttcccctgat cagggttttg acttccgttt
    10321 ccgctctccc gcagaaggag cagctcaggt tcttatattt atccaaacat tcctccctgg
    10381 tttgcctggt tcgttgtatg taatgtcgga ttccggtacg actgattcgt gagtgtcgga
    10441 ttccgttccg gcagttcata tgcaaggtcg gctctggcgg ccttgctgtc aagtttattt
    10501 tcgttgactg atcacctcgt cgatcagtcc gtaggctttg gcctcatcag cgctcataaa
    10561 gaagtttctg tctgtgtccg cttcgatgcg ttccagaggc tgaccagtgt gtttttgcat
    10621 cagttcattc atcacccgtc tcagatagag gatttccttg gcctggatct cgatatcgct
    10681 ggcctgtccc tgagcgcctc ccagaggctg atggatcata acgcggctgt aaggcagagc
    10741 ggtccttttt ccaggagctc cagccgcgag cagcatggca gccatactgg cagcctgacc
    10801 aatgcagatg gtactcactt tgggctttat atactgcatc gtgtcataga ttgccaggcc
    10861 ggacgatatg atcccaccgg ggctgttgat atacatatag atgtcgcgct ccgcgtcttc
    10921 accttccagg tgcagaagct gggctacgat cgaattggcc agattgtctt caattactcc
    10981 gctcacaaac acgatccggt ctttgagcag cctggaatag atatcgtagg cccgttccac
    11041 gcggcctacc tgttcgatta ccatagggat gtagttcatg cgttctcctc atttacttcc
    11101 ggattctctt cattcggctc cactgcaggc ggttccggtt tcacaaattc ggccctggcg
    11161 gccaggtttc tcagcattgt ccagctacgg gcctcgtctt tgaagtcttc tgtactcagc
    11221 cgttcggcat gtttttcttt ccatgctccc acggggatat cttccagaat ggcttcatgc
    11281 tcgatatact ggtcaagcat ggcgtcgtcg agagtggggt tcagcaagcg gttcagcgct
    11341 ttcatgatat agacattgat catctgctgg ctaaactgca tgcggaattg atattcgtag
    11401 tattgctgat aacgtttgtc ttcgatcttg gccgcttctt ccctggcgag gtattcgatg
    11461 gtgcgcttgg gcagatcgaa aggaccatcg gcatagaatt tgtggatgat ggcgttattc
    11521 tcggcgtcga aattgttgtg ttccaggcgc agcttcatgt ttgaggcgat ctcagctttg
    11581 agcgaggcca ggtcttcatg gtccatgtct ttggcaaatt cgtcatcgat ggccggctcc
    11641 acatatctgc ggatgtcgtt taccatcagg ctgcagggat agctggtgtc gttgtcgagc
    11701 ttgtctatgc ttgttctggt gaccagtttg agggagttgc cgctcagctg ggtttccacc
    11761 acgtcaccca cttttttgcc aaccagctca gccactgagc gttgcggcat ggcgtctccg
    11821 gcgaagagga ctcccgtatg ggtcaaggtc tcgtcgttta ctgctatttt tatctcacac
    11881 tccaccgcgt catccgcgct tacttcctcc acctgaagat aggtggcgtt ttcctgggcc
    11941 atctttttga ggaagcgctc cacctcatcg tctaaagaga tggcttcatg cggtaccacc
    12001 agaggatctg gaatgctgag ctccacctcc ggttcgtgtt cgatctcgat ctcggcctta
    12061 aattcagagc ctttctccca ctggatgtct ttgacgtggg gcatcagaag atatccaagc
    12121 tgatgctctt tgatcacttc attgaaataa tcatctgtgg cgtctttgag aaaataatct
    12181 ttcacggtct cgccgtacat ccgttccacc atgggaagtg gggctttgcc cttgcggaat
    12241 ccagggatct ggatgtccct ggcgctgcgg ctgaggaatt tttgatagtc tctggctgcc
    12301 gtttccgcgt cgattgtgat cgtgatgctc ttggtgacgg gatcgagctg tttgatatct
    12361 gtctgcactt tattctcctt gaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnncttggt gacgggatcg agctgtttga tatctgtctg cactttattc tccttgaatg
    12541 aatctcttgc accccaaacg tcctcgtttg cggttgttgg tcagcgttcg tcgaatcgga
    12601 attcggcgtt ccatgtttcc acgggggtcc acacgtatgc gtctttctaa actggtgcga
    12661 aagaggggac ttgaacccct acaggataaa cctactggat cctaagtcca gcgcgtctgc
    12721 caattccgcc actttcgcac aatgggcatg gggatctctt gatccccgga tattttagtc
    12781 ggtgacaatt atttgaagag gccctggatg tcaagagccg agtgccccat tgtcgatcaa
    12841 tctggtccgc ccgataaaga cggccatcac catccgggta tctgcttcag ccgtttcaac
    12901 cggagtaagc agtttaggat caacgcattc cacatagtcg atgtttgcac ccgcagtttc
    12961 aatcaggcgg ctggcttcag caatcaacac cgcggtgccg cgttctccca gggcaaaact
    13021 atgtctggca gtgattaagg cctggcttag gcagcgcgcg cggattctct catcggcgtc
    13081 caaatagctg ttgcggctgc tcatagccag tccgtccgtt tcccggatga tcggacagcg
    13141 ggtgatcacg gtatcgtggt tgaggtccgc gagcatgcgc tccagcacca ctacctgttg
    13201 aaaatccttg ctgcccatat acatatagtc cggttgaact atatttacca gcttcaaaac
    13261 gatcgtcgcc acccctctga aatggccggg acggctcttt ccgcagagcg gaaccgagag
    13321 cttttcgacc tccacccagg tctgatgttc agagggatag atttccgcgg catcaggata
    13381 gaatacgtaa tccgtacccc tgctttgcag cagctccaga tcgcggtcca gatcacgagg
    13441 ataggtggag aggtcttcat ttctgccaaa ctgggcggga ttcacaaaga tggaaaccac
    13501 cgtgtggcgg tttgcgctca cgctttggtc caccagcgat aggtgtccct catgcagata
    13561 acccatggtg ggcacgaaac ccaggctgcc ttcccgtttc agagactggc acacagcctt
    13621 catttccgtg atgctgttga taacccgcat ttacctgact ggctggttgt ggtcgtccag
    13681 gatcacaatt cggggctgat gtccttctat ctctttatca tccataaggc cgtaattgat
    13741 gatgatcacc ttatcaccca ccatggccat tctggcggcc gcgccattga tcgcgatttc
    13801 cccgcgaccc cgttctcctt cgatgatata ggtggaaaaa cgcgcgccgt tgttgacgtt
    13861 gaagatctct accttctcaa aaggacgcat gccagccgcg tctaaaagat ccggatcgat
    13921 cttgatgctg ccgttgtagt tcaggtcaca ttccgtgaca gtgccacggt gtaacttcga
    13981 taaaagtata tatcttagca ttgaattctc caggttgtag cttgtgagtc atcattttag
    14041 acggcgcctt tgtgtcaagt gctttccctt atctatatat gtcacaccaa acttgagcac
    14101 caggcagaat ctcatggccg atacaaggta cgtgaagatg ctgatcgatg atttcctggt
    14161 tcctgctcat cacccgcggt agaactgccg tgcggcctcc cgggtttttc gccggagagc
    14221 gcacggtatc ccaacggcag ttgataagga aggatgtggg gaagagggag tcgtgtgatc
    14281 tctatcgaga ctatcagaat ctcgtttaca ggattgattt gcccgggacg gtgcttcctc
    14341 ccttacccat gtaccctttg ccgccccaaa tctcgtccta cccaaaccga gctttattgg
    14401 ggatgggtac gagatgctgt aaccggccac aggtgcaagc ggtaagtgcg gaacagccaa
    14461 gagcctgtaa ctcactcaaa gacatgaaca tattccggca atgaccaatg cggagttgta
    14521 tcgctggctt cagccggccg tagcactgac ttcagtcggt gcgagaaaat caggctataa
    14581 tctaatatga taagagttta cctattgaat accccgtcgc gtagggtacc ggctagagcc
    14641 ggcactacga ccggctaaag ccaatgttac ataatccttt acgccatatg gttgtcatca
    14701 gatcacaccc caaaacaaaa accccggaac ctgtgaaaag tcccggggct gtaatcagtc
    14761 agccttgcaa ggcgatcact tcatca
//
LOCUS       DAOSFN010000053        14518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3421_length_14518_cov_9.414160, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000053 DAOSFN010000000
VERSION     DAOSFN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 14518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14518
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3421_length_14518_cov_9.414160"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1292
                     /gene="tuf"
                     /locus_tag="PLO35_04890"
     CDS             108..1292
                     /gene="tuf"
                     /locus_tag="PLO35_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOH79036.1"
                     /translation="MAKEKFVRTKPHVNVGTIGHIDHGKTTLTAAITLYLSKKGGAKF
                     RSFDSIDNAPEEKARGITIATAHVEYESDNRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAYDGPMPQTREHILLARQVGVPAVVVFMNKCDLVEDPELLDLVEMEVRELLDK
                     YEFPGDECPVIRGSALKALNGDPEGEAQIQELVDAIDTYIPLPERATDKPFLMPVEDV
                     FSIPGRGTVATGRVERGVIKIQEKVERVGIRETVETTCTGVEMFRKLLDEAQAGDNIG
                     VLLRGFAKTDVERGMVLAKPKSITPHTKFSGQTYVLTKDEGGRHKPFQDGYRPQFYFR
                     TTDVTGTVHLPEGVKMVMPGDSVTVIGQLITPIAMEQGLRFAIREGGRTIGSGVVGTI
                     IE"
     gene            1299..1445
                     /gene="rpmG"
                     /locus_tag="PLO35_04895"
     CDS             1299..1445
                     /gene="rpmG"
                     /locus_tag="PLO35_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOH79037.1"
                     /translation="MRDIIVLACEDCKNRNYTTTRNKRKHPNRMELKKFCPTCRKHTV
                     HKQR"
     gene            1466..1541
                     /locus_tag="PLO35_04900"
     tRNA            1466..1541
                     /locus_tag="PLO35_04900"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1499..1501,aa:Trp,seq:cca)
     gene            1746..1931
                     /gene="secE"
                     /locus_tag="PLO35_04905"
     CDS             1746..1931
                     /gene="secE"
                     /locus_tag="PLO35_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOH79038.1"
                     /translation="MKFDKVTRFFHDVRSEMRSVTWPTKADLKEGTIVVIVMSALVAI
                     FLALVDLGFSQIVELVF"
     gene            1959..2657
                     /gene="nusG"
                     /locus_tag="PLO35_04910"
     CDS             1959..2657
                     /gene="nusG"
                     /locus_tag="PLO35_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOH79039.1"
                     /translation="MKPAKEPTPGIAPQDGPSTSPAEEKEDLGLGAPAGSQLERREDD
                     GAQSPQASVSNKKWYVVHTYSGHENKVKTAIEKGLEGTELKDLVGQILVPVRKTFIIR
                     EGKKIDRERKLFTSYVIIEAELNPALRAYILNIAGVTKFLGMAKDHKDPTPLPEDEVN
                     RLLGIADRDPEAKAFSFMPGDAVRVTAGPFSEFEGVVTKAADDGTKLVIDVTVFGRRT
                     PVELNSNQVEVIKK"
     gene            2677..3108
                     /gene="rplK"
                     /locus_tag="PLO35_04915"
     CDS             2677..3108
                     /gene="rplK"
                     /locus_tag="PLO35_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOH79040.1"
                     /translation="MAKPKDVVAMIKLQLPAGKATPAPPVGPALGQAGVNIPEFCRQF
                     NEKTADQPGMVFPVVISVAKNKSFTFEIKTPPASVLIKKAAGLAKGSSTPNKAKVGKL
                     TQDQLRDIARLKIQDMNCETLESAMSMIAGTARSMGVTTEN"
     gene            3149..3853
                     /gene="rplA"
                     /locus_tag="PLO35_04920"
     CDS             3149..3853
                     /gene="rplA"
                     /locus_tag="PLO35_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOH79041.1"
                     /translation="MKHSKRYTHAYAMYDRQKRFDLDEALKILKTIPASKFDETVEVH
                     FNLGVDPRKADQQIRNSLVLPHGTGKTVRVLVFAEGDKADEARKAGADYVGLDDLVEK
                     ITGGWFDFDMVITTPNLMGRIGKLGRVLGPRGLMPNPKVGTVTMDIVKAVDEAKGGKV
                     TYRVDKFANLHIPAGKISFSPEQLKENIKAILAAILKERPATMKGVFIKSITLCTTMG
                     PGIKLQVASATLEAKS"
     gene            3866..4402
                     /gene="rplJ"
                     /locus_tag="PLO35_04925"
     CDS             3866..4402
                     /gene="rplJ"
                     /locus_tag="PLO35_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOH79042.1"
                     /translation="MVQSVKYDLVTHLKERLADAKAIVLVDYKGINIEQVNQLRRTYR
                     AGKVDYFVQKNTLIKLALNDLGITELDAHLMGPTAVAVCHEDEVAPARVLVKFLKDVM
                     EDAPFPSFKAGFVSGQLLDAKQLQALAAMPSREELLAKVLGSAQAPITNFVSVTQGII
                     RKFVYAVDAIRKKNAEAS"
     gene            4436..4816
                     /gene="rplL"
                     /locus_tag="PLO35_04930"
     CDS             4436..4816
                     /gene="rplL"
                     /locus_tag="PLO35_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOH79043.1"
                     /translation="MSDKQQQVIELIKGMTVLELSELVKEMEEIFGVSAAAPVAAFAG
                     PVAGAAAEAAEEQSEFDVILANAGDKKINVIKVVREITKLGLKEAKDLVDGAPKMVVE
                     KVSKEEAESVKKKLEEAGASIELK"
     gene            5019..8807
                     /gene="rpoB"
                     /locus_tag="PLO35_04935"
     CDS             5019..8807
                     /gene="rpoB"
                     /locus_tag="PLO35_04935"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOH79044.1"
                     /translation="MRKIKSYSRISGRFAELGIPEVEIPNLLAMQVDSFNEFLQKDVH
                     PQRRAKQGLQAVFEAIFPIEDVKGNFLLEFMEYNVLQEKYSIDECRERNLSFQAPLKA
                     KMRLSVFEQNEGVREHKDTIEQDVFLGEIPLVTEQGTFVVNGAERVIISQLQRSPGVF
                     FSEEKHPSGKTLYSAKVIPYNGSWLEFDIDIHDVMYVHIDKRRKLPVTILLRAIGIST
                     NHDLRKVFYQSESLGLKEAKGRHLYQDITVTGVPEPIAFANEQITDSLIAILESHQIR
                     KVEVIDYNFEIARKVLENTIAKDPTTNQEDALHKIYSLIRPGEEAPLEAARQLVDRMF
                     FNEKRYNLGEVGRYKINTRLGIDIDPSVRTLCVDDFVHIFKTLINIYHDLDEVDDIDN
                     LSNRRVRTVGELLQEQYTSGLSMVARIIQERMSISNTDEITVHDLVNSNALISVVQSF
                     FLTGQLSQFMEQTNPLAALRHKRALSALGPGGLTRDRAGFEVRDVHASHYGRVCPIET
                     PEGPNIGLIVSPSIYSRINHLGFIETPYRKVVNGKVTDDYVYMDAAQEEKYIIGQSDI
                     DLDDDRRIMNETIFARERGEFIQVRPDQVEFMDVAPQQMVSVSAAMIPFLEHDDANRA
                     LMGSNMQRQAVPLINPQAPLVGTGMEKIVTMDTSTVAVAPYDGVVTNVTSAYVEIKPL
                     NEKDEAFYLSTANRVELKKFIRTNQDTCVNQRPIVRIGDTVRKGQAVSDGACVEDNRL
                     ALGTNMLVAFMPWYGYNYEDAIILSEKVAREDTLTSIYIEEMEVLVRNVKNGREELAY
                     DIPNVPAQALRNLDKTGIIRVGSVIHAGDIIVGKVTPKSIEIDPSPEENLMRALFGDR
                     AGDFTNSSLKAKPGMEGVVIDVKVFSRNEDGTEQDEDMDEKILKLKSDLNIRRKKVED
                     FKEEKLTAALLGEVAKTIWDEKSNVYFIAPGKKITADDIARINFRKLDLDVELVEDMA
                     KNELIYGQIILKIKQSLEQSDNVYRKARERIRHGDELQYGVRKMVKVYVAKKRKIEVG
                     DKMAGRHGNKGVISIVAPIEDMPFMEDGTPVDIVLNPLGVPSRMNIGQIMETHLGMAA
                     RALGFEVETPIFDGATVDDIQSELKNAGYAPDGKQVLYDGKCGEPFKERVTVGVIYMM
                     KLNHLVADKMHARSTGPYSLITQQPLGGKAQHGGQRLGEMEVWALEAYGAAHLLEEML
                     TIKSDDVDGRNNAFKAITRGDNPPPPGVPESFNVLISELKSLGFDIEFIKESEDTQGR
                     "
     gene            8809..>11999
                     /gene="rpoC"
                     /locus_tag="PLO35_04940"
     CDS             8809..>11999
                     /gene="rpoC"
                     /locus_tag="PLO35_04940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOH79045.1"
                     /translation="MLKDNRREIRPNDYDFVRIRIASPDTIVNSWSHGEVTKPDTLNY
                     RTLKPEKDGLFCERIFGPERDYECACGKYKKKRFQNTVCDRCNVLVTTSRVRRTRMGH
                     IQLAVPIAHIWFVKSAPSKIGTLLDMTVKDLERVLYYESFIVIDPGDSPYEKFELIEV
                     DEYYEIRDKVGDNFIAMMGAEAVRELLTRIDLKNEALNIRSRLKMEKATLHKQKLINK
                     LKIVDAFIKSGNKPEYMIMEALPVLPPTLRPLVPLEGGRFATADFNDLYRRVITRNNR
                     LKQLLEIRAPEVILRNEKRMLQEAVDALIDNSRKPRPVRGRGNRPLKSLTDQLKGKQG
                     RFRQNLLGKRVDYSGRSVITVGPELKLYQCGIPKDMAVELFKPYLIERLQKLGEVDKV
                     KNAKKLIEKKQPEIWSILEDVIKDYPVLLNRAPTLHRLGIQAFMPVLTDNKAIQLHPM
                     VCVPFNADFDGDQMGVYVPLGVEAQIEARVLMLSTRNLLLPANGRLAMAANQDIVLGC
                     YYLTMEAGDAPADTTSLRHFYGPVEVITAYEAEEQLCSVKGHSEVERNLDLHSWIRVR
                     IGSELITTTVGRVIFNQIIPQEVGYQNITYDKGKLNDLAMLSYDAVGQWRTAVMLDDL
                     KELGFRYATRAGVTFSFGDIVVPARKGEIIAQSEAEVKKVFDLHQKGGITENERVGRI
                     VDLWKKTTVRVTDEMMEELTHSRQGLNSIYMMYKSGARGSKDQIKQLGGMRGLMDKPS
                     KIGSTGGADVIETPIKSNFYEGLTVLEYFVSTHGARKGLADTALKTADAGYLTRRLVD
                     VAQNAIITLEDCGTGRGVHMSVLKEGNEIVQSLAERIQGRVSLEDVVDPVTGKVLVNA
                     GDEISNKMARTIQNHGIISVYVRSVLTCEAERGICAKCYGRNLATQKPATIGDPMGII
                     AAQSIGEPGTQLTLRTFHIGGAASTATELAEVVSNHDGIVKFDRMNTVTNIEGHLISV
                     SHLGKIILVEAEDETRVLEEYKVEYAATVHVQDGQKIVTNTRLLSWDQFNNPLISTAK
                     GVLHYDNFIKDITFKEEYNDITYSRDLTIIESK"
     assembly_gap    12000..12099
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12100..13155
                     /locus_tag="PLO35_04945"
     CDS             <12100..13155
                     /locus_tag="PLO35_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOH79046.1"
                     /translation="DIDGRVTIGGLKKTGRDIFVTPSNGLFAPADGKVLVQSDELRNR
                     VVLLTDKAILAANDGKVSIHEDSKSRTVTVTKRGGKGEEYKIPRSFQIIVAPDQSVKT
                     GAPLGGLVFDIPELQSPIVETGDAVQKGQALAGRKYSIPSGKRIIVHQGDYVESGDAL
                     SDGPLDPHDMLVKGIIPAQLMILNEIQEIYRKQGVKIDDKHVSVIIRQMFKKLRITDP
                     GSTSFLEGDIVDKTAVERENREVLQYGKAPAQSEQLLLGITKTSLLTQSWLSSASFQE
                     TTKVLTRAAIEGSVDHLEGLKESIILGHRIPVGTGTRIYNQRIEQAVGEGKTVAQIIN
                     SFAHPDTEPESEDIMDF"
     gene            13195..13551
                     /gene="rpsL"
                     /locus_tag="PLO35_04950"
     CDS             13195..13551
                     /gene="rpsL"
                     /locus_tag="PLO35_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOH79047.1"
                     /translation="MPTINQLIRKGRTEALMGCPQRRGVCTRVYTTTPKKPNSALRKV
                     ARVRLVNGFEVTAYIGGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGALDSAGVEKR
                     VNSRSKYGTKRPKVKK"
     gene            13565..14035
                     /gene="rpsG"
                     /locus_tag="PLO35_04955"
     CDS             13565..14035
                     /gene="rpsG"
                     /locus_tag="PLO35_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOH79048.1"
                     /translation="MPRKRKAVVREVLPDPKYNDVVLTKFMNCLMVKGKKSLAEKIVY
                     GAFDIIAAKTKQPPLEVFKAALDNVRPLVKVVSRRVGGSNYQIPMEINEKNGRALAFR
                     WLISYARNRSEKTMIEKLAGELMSAYKKEGSSIKKREDTHKMAEANKAYAHLRW"
BASE COUNT         3691 a         4125 c         3873 g         2729 t
ORIGIN      
        1 ttggtaagga ggaggtcatc ggttcgagtc cgatcttgag ctccactttc tttttgatta
       61 cgaaataaaa cacgacatgg agtgccggac gcgctccagg aggaacaatg gcaaaagaaa
      121 aattcgtacg taccaagccg cacgtcaacg ttggtacgat cggtcacatc gaccacggta
      181 agaccactct caccgcggcg attactttgt atttgagcaa aaagggcggc gctaaattcc
      241 gttcattcga cagtattgac aatgctccgg aagaaaaagc ccgcggtatc accatcgcca
      301 ctgcccacgt cgaatatgaa tctgacaatc gtcactatgc ccacgtggac tgcccaggtc
      361 atgctgacta catcaaaaac atgatcaccg gtgcggccca gatggacggt gccatcctcg
      421 tcgtgagcgc ttatgacggc ccgatgcccc agacccgcga acacatcctg cttgcccgtc
      481 aggtcggcgt gcccgccgtc gtcgttttca tgaacaaatg cgacctcgtc gaagatcccg
      541 aactgcttga cctcgtcgaa atggaagtcc gcgagctgct ggacaagtat gaattccccg
      601 gagacgaatg ccccgtgatc cgcggttccg ctctcaaagc cctcaacggc gatcccgaag
      661 gcgaagctca gatccaggag ctggtggatg ccatcgacac ctatatcccg ctgccagaac
      721 gcgccacgga caaacccttc ctgatgcccg tcgaagacgt cttctccatc cccggacgcg
      781 gcaccgtcgc taccggcaga gtggagcgcg gcgtcatcaa gatccaggaa aaagtggagc
      841 gcgtgggtat ccgcgagacc gtggaaacca cctgcaccgg cgtggaaatg tttcgcaagc
      901 tgctcgatga agcccaggcc ggcgacaaca tcggagtcct cctgcgcggc tttgccaaga
      961 ccgacgtgga acgcggtatg gtgctggcca aacccaaatc catcaccccc cacaccaaat
     1021 tctccggcca gacctacgtc ctgacaaagg acgagggtgg acgtcacaaa cccttccagg
     1081 atggctaccg tccccagttc tatttccgta ccacggacgt gactggcacc gtgcacctgc
     1141 ctgaaggcgt caaaatggtg atgcccggtg acagcgtcac cgtgatcgga cagctcatca
     1201 cccccatcgc catggagcaa ggcctccgct tcgccatccg cgaaggtgga cgcaccatcg
     1261 gcagcggcgt ggtgggaacc atcatcgagt aaaggaccat gagagatatc atcgtactcg
     1321 cttgcgaaga ttgcaaaaac cgcaattata ccaccacccg caacaaacgc aagcacccga
     1381 acaggatgga gctgaaaaaa ttctgcccca cctgccggaa acacacagtt cacaaacagc
     1441 gctaggcgct gataagatat gttgcaggac agtagttcaa ctggtagagc accggtctcc
     1501 aaaaccgggg gttgcgggtt cgattcctgc ctgtcctgcc acgttccttt taacgggttt
     1561 agaatgggga tttgtttccc gcggattacg cggattggta cgcagatctc gcagattttg
     1621 agatggtttg gagggtttaa gcctttggat gctggatctt ctcaaccttt acaaaatcag
     1681 agagatccgc gccatccacg gtcaaactac ccatccacct caagctcatt ttcgcaggta
     1741 taacaatgaa attcgacaaa gtaacccgct tcttccacga cgtgcgctcc gaaatgcgct
     1801 ccgtgacctg gcccaccaag gccgacctga aggaagggac catcgtggtg atcgtgatgt
     1861 ccgccctggt ggcgatattc ctcgctctcg tagatctggg attcagccag atcgttgaat
     1921 tggtttttta acttaagatg caagccaaaa agaaaagcgt gaaacccgcc aaagagccga
     1981 cgcccgggat cgctccgcaa gacggccctt ccacctcacc cgcagaggaa aaagaggatt
     2041 tggggcttgg agccccggcc ggttcacagc ttgagcgacg cgaagatgac ggggcccaaa
     2101 gcccccaagc cagcgtgtca aacaaaaaat ggtatgtggt acatacctac tccggccatg
     2161 aaaataaggt gaagaccgcc atcgaaaaag gcctggaagg cacggaactc aaagatctgg
     2221 taggtcagat tctggtcccg gtgcgcaaga cctttatcat ccgcgaaggc aaaaagatcg
     2281 accgcgaacg taagctcttt accagctacg tgatcatcga agcggaactc aatcccgccc
     2341 tgcgcgccta catcctcaat atcgccggag ttacaaagtt tctggggatg gcaaaggatc
     2401 acaaagatcc caccccgctt cccgaagacg aggtcaatcg cctcctggga atcgccgatc
     2461 gcgatcccga agccaaggcc ttcagcttta tgcccggaga cgcggtgcgc gtcacggccg
     2521 ggcccttttc agaatttgaa ggcgtggtca ccaaggccgc tgacgatggc accaagctgg
     2581 tgatcgatgt caccgtgttt ggacgccgca ccccggtgga attgaattcc aaccaggtag
     2641 aagtaattaa aaagtaatat catagaggtt atcataatgg caaagccaaa agacgtcgtc
     2701 gccatgatca agctgcagct tcccgccggg aaagccaccc ccgctcctcc cgtcggaccc
     2761 gctctgggac aggccggagt gaatattccg gaattctgcc gccagttcaa tgaaaagacc
     2821 gcagaccagc ccgggatggt gtttcccgtc gtgatctcgg tggcgaaaaa caaatccttc
     2881 acctttgaga tcaaaacccc tccggcctcc gtgctcatca aaaaagccgc cggcctggcc
     2941 aaagggagtt ccactcccaa caaagccaaa gtaggaaaat tgacccagga tcaactgcgc
     3001 gacatcgcgc ggctcaagat ccaggatatg aattgcgaaa cccttgaatc cgctatgagt
     3061 atgatcgcag gtactgcaag gagcatgggc gtcaccactg aaaattgatg cggcctgtgc
     3121 aggagaacat attcattaag gagagtcaat gaaacatagt aaaaggtaca cccacgccta
     3181 cgcgatgtac gaccgccaaa agcgcttcga tctggacgaa gcgctcaaga tcctgaagac
     3241 catcccggcc tcaaaattcg acgagaccgt ggaagtgcac ttcaatctgg gcgtcgaccc
     3301 gcgcaaagcc gatcagcaga tccgcaactc cctcgtgctc ccgcacggca ccggtaaaac
     3361 cgtgagagtg ctggtcttcg ctgaaggcga taaggcagac gaagccagaa aggctggcgc
     3421 cgattacgtc ggattggatg atctggtaga aaagatcacc ggaggatggt ttgactttga
     3481 tatggtcatc accactccca atctgatggg acggatcgga aaactgggaa gagtgttggg
     3541 tccccgcggc ctgatgccca atcccaaagt cgggaccgtt accatggaca tcgtgaaagc
     3601 ggtggacgag gctaagggcg gaaaagtcac ctacagggta gacaaattcg ccaatctgca
     3661 tattcccgcc gggaagatca gctttagccc cgaacagctc aaagaaaaca tcaaagctat
     3721 cctggccgcc atcctcaaag aacggcccgc cacgatgaaa ggcgtgttta tcaagagcat
     3781 caccctctgc accaccatgg gccccggcat caaactacag gtcgcgagcg caaccttgga
     3841 agctaagagc taagggagag atcgaatggt tcaaagtgta aaatacgacc tcgtcacaca
     3901 cttgaaagag agattggcag acgctaaagc gatcgtactg gtggattaca agggaatcaa
     3961 catcgaacag gtaaaccagc tccggcgtac ttacagggcc ggcaaggtgg attacttcgt
     4021 gcaaaaaaac accctgatca aactcgctct caatgatctg gggatcacag aactagacgc
     4081 tcatctgatg ggtcccaccg ccgtggcggt ttgccatgag gatgaagtag cgcccgcccg
     4141 ggtgcttgtg aaattcctga aagacgtgat ggaagacgct cccttcccca gcttcaaggc
     4201 tggtttcgta agcgggcagc tcctcgatgc caaacaactg caagccctgg ccgcgatgcc
     4261 ttcccgcgaa gaactcctcg ccaaggtcct cggcagtgct caggcaccca tcaccaattt
     4321 cgtgagtgtg actcaaggca tcatccgcaa gtttgtctat gccgtggatg ccatccgcaa
     4381 gaaaaacgcg gaagccagct aaccctaaaa gaattataat accctggagg aaaagatgtc
     4441 cgataaacaa cagcaagtaa ttgaattgat caaagggatg accgtgctgg aactctctga
     4501 actggtcaaa gaaatggaag aaatatttgg cgtgtccgcc gccgctcccg tggccgcttt
     4561 cgcgggtccc gtggccgggg ctgccgccga agccgctgaa gaacaaagcg aatttgacgt
     4621 gatcctcgcc aacgccggcg acaaaaagat caacgtgatc aaagtagtcc gcgaaatcac
     4681 caagctggga ctcaaagaag ccaaagacct cgtcgacggc gctcccaaaa tggtggtaga
     4741 aaaagttagc aaagaagaag ccgaatcggt gaagaagaaa ctcgaagaag ctggtgctag
     4801 catcgaactc aaatagggga tcacttcccg aaactgctgt ccaaaaaaag tccccaggga
     4861 ctttttttgg actcattatg ctcaagaacg gcaaacaatt cagcgcgggg cccggactca
     4921 ctgtcaaggt gacgtccggg acctttggcg cgcgtctcac aacccccaac gccagatacc
     4981 tctatgcgta ggtataacaa tcgcaaagga gtgagctttt gagaaagatc aaaagttatt
     5041 cccgtatctc cggaagattc gcggagcttg gaatcccgga agtggagatc cccaatctgc
     5101 tggccatgca ggtggattca ttcaatgaat tcctccagaa ggacgtccat ccccagcgcc
     5161 gggccaaaca aggtctccag gccgtcttcg aggccatctt cccgatcgaa gacgtcaagg
     5221 gcaatttcct gctcgaattc atggaataca acgtgttgca ggagaaatac tccatagacg
     5281 agtgccgtga acgtaacctc tcatttcagg cacccctcaa ggccaagatg aggctcagcg
     5341 tgttcgaaca gaacgaaggc gttcgcgaac acaaagacac catcgaacaa gacgtcttcc
     5401 tgggtgaaat cccgctggtt acagagcagg gcactttcgt ggtcaatggc gccgagcggg
     5461 tgattatctc tcagctccag cgctccccgg gggttttctt ttccgaggaa aaacatccca
     5521 gcggcaaaac cctctactct gccaaggtca tcccctacaa cggctcctgg ctggagtttg
     5581 acatcgatat ccacgacgtc atgtatgtcc atatcgacaa gcgtcgcaaa ctgcccgtca
     5641 ccatcctgct cagggccatc ggtatatcca ccaatcacga cctcagaaag gtcttttacc
     5701 aaagcgaatc gcttggtctg aaagaggcga aaggccgcca tttatatcag gatatcaccg
     5761 tcaccggggt cccggagccc atcgcgtttg ccaacgaaca gatcaccgat agcctcatcg
     5821 ccatcctgga atctcaccag atccgcaagg tcgaggtgat cgactacaat ttcgagatcg
     5881 cccgcaaggt gctggaaaac actatcgcca aagatcccac caccaaccag gaagatgctc
     5941 tccacaaaat ctacagcctc attcgccccg gtgaagaagc tcccctggaa gctgcccgac
     6001 agctggtgga ccgcatgttc ttcaacgaaa aacgttacaa cctcggtgaa gtaggccgct
     6061 acaagatcaa tacccgcctg ggcatcgata tcgatcccag cgtccgcacc ctctgcgtgg
     6121 acgactttgt gcatatcttc aaaaccctga tcaatatcta tcacgacctg gatgaggtgg
     6181 acgatataga caacctgtcc aaccgccgcg tccgcaccgt gggcgagctc ctccaggagc
     6241 agtacacatc agggctttcc atggtggcgc gcatcatcca ggaacgcatg tctatctcca
     6301 acaccgacga gatcaccgtg cacgatctgg tgaacagcaa cgccctgatc tccgtggtgc
     6361 agtccttctt cctcaccgga cagctctcgc aattcatgga acagaccaat cccctggccg
     6421 ctttgaggca caaacgcgcc ctctcagccc tgggaccagg tggcctgacc cgcgaccggg
     6481 ctggctttga agtccgggac gtgcacgcct cccactatgg cagagtgtgc cccatcgaga
     6541 ctcccgaagg acccaatatc ggtctgatcg tgtcgccctc catctattcc aggatcaatc
     6601 acctgggctt catcgaaacg ccctaccgca aagtggtaaa cggcaaggtg acagacgatt
     6661 acgtctatat ggacgccgcc caggaagaaa agtatatcat tggccagtcc gacatcgacc
     6721 tcgatgacga ccgccgtatc atgaatgaga caatctttgc ccgcgaacgg ggtgagttta
     6781 tccaggtgag gccagaccag gtcgagttta tggacgtcgc gcctcagcag atggtttccg
     6841 tttccgcggc catgatcccc ttcctggaac acgacgacgc caaccgtgcc ctgatgggct
     6901 ccaatatgca gcgccaggct gtgccgctga tcaatcctca ggccccgctg gtgggcaccg
     6961 ggatggaaaa gatcgtgacc atggatacct ctacggttgc cgtggccccc tatgacggag
     7021 tggtcaccaa cgtcacttcc gcctatgtcg agatcaaacc tctcaacgaa aaggacgagg
     7081 ctttctatct cagcactgcc aaccgcgtcg agctcaagaa gttcatccgc accaaccagg
     7141 atacctgtgt aaaccagcgt cccatcgtcc gcattggcga taccgtccgc aaaggccagg
     7201 ccgtttcaga cggtgcctgc gtggaagaca accgtctggc cctgggcacc aacatgctgg
     7261 tagcctttat gccctggtat ggttacaact atgaagacgc catcatcctc agcgagaagg
     7321 tggcgcgcga agacaccctc acctccatct acatcgaaga gatggaagtg ctggttcgca
     7381 acgtcaagaa cggccgggaa gaactggcct atgatatccc caatgtaccc gcccaggcac
     7441 tccgcaatct ggacaagacc ggcatcatcc gcgtgggttc tgtgatccac gccggcgaca
     7501 taatcgtagg taaagttact cctaaaagca tcgagatcga tccctctcca gaggagaatc
     7561 tcatgcgagc tctgtttggc gaccgggccg gtgactttac aaatagctcc ctcaaggcca
     7621 aacccggcat ggaaggagtg gtcatcgatg tgaaggtctt ctcccgcaat gaggacggca
     7681 ccgaacagga cgaggacatg gacgagaaga tactcaagct gaagtccgac ctcaatatcc
     7741 gccgcaaaaa ggtggaggac ttcaaggaag agaagctcac agccgccctg ctgggtgaag
     7801 tggccaagac catctgggat gaaaagtcca acgtttactt cattgctccc ggcaagaaga
     7861 tcactgccga cgacatcgcc cggatcaact tccgtaagct ggacctggat gtcgagctgg
     7921 tggaagatat ggccaaaaac gagctgatct acggccagat aatcctcaag atcaagcaat
     7981 ccctggagca gagcgacaac gtctatcgca aggcccgcga gcgtatccgt cacggtgacg
     8041 agctccagta tggcgtgcgc aagatggtca aagtctatgt ggccaagaag cgcaagatcg
     8101 aggtgggaga caagatggcc ggtcgtcacg gcaacaaagg cgtgatctct attgtagccc
     8161 ctatcgagga tatgcccttt atggaagacg gcactcccgt cgatatcgtt ttgaaccctc
     8221 tgggtgtgcc ctcccgtatg aacatcggcc agatcatgga gacccatctt ggcatggcag
     8281 cccgggctct gggatttgag gtggaaaccc ccatctttga cggtgctact gtggatgaca
     8341 tccaatccga gctcaaaaac gccggttacg ctccggacgg caagcaagtg ctatacgacg
     8401 gcaaatgcgg cgagcccttc aaggagagag tgacggtagg cgtgatctat atgatgaagc
     8461 tcaaccacct ggtggcggac aagatgcatg cccgctccac gggtccctat tccctcatca
     8521 cgcagcaacc tctgggcggc aaagcccagc atggcggcca gcgtctggga gagatggaag
     8581 tctgggccct ggaggcttat ggcgcggctc atctgctgga agagatgctc accatcaaga
     8641 gtgacgacgt ggatgggcgc aacaacgcct tcaaggctat caccagaggc gacaatccgc
     8701 cgcctccagg ggtgccggaa tcatttaacg tgctgatcag cgagctcaag tctttgggct
     8761 ttgacatcga attcatcaaa gaatcagaag atactcaggg gaggtaacat gctgaaagac
     8821 aaccgacgcg agatcaggcc caacgactat gactttgtgc gcatcaggat cgcctctccg
     8881 gacacgatcg tcaattcctg gtcgcatggc gaagtcacca aacccgatac cttgaactac
     8941 cgcaccctca agcccgagaa ggacggcctc ttttgcgaac gcatctttgg ccccgagcgg
     9001 gattacgagt gcgcctgcgg caagtataaa aagaaacgct tccaaaacac cgtctgcgat
     9061 cgctgtaacg tgctggtaac cacctcccgg gtgcgtcgta cccgcatggg ccacatccaa
     9121 ctggcggttc cgatcgccca catctggttt gtgaagagcg ctcccagcaa gatcggcacc
     9181 ctgctggaca tgacggtcaa agacctggaa cgggtgctgt attacgaatc ctttatcgtg
     9241 atcgacccgg gtgacagccc ctatgagaag tttgagctca tcgaagtgga tgaatactat
     9301 gaaatccgcg ataaagtggg cgacaacttc atcgccatga tgggagccga agcggtgcgc
     9361 gaactgctga cccgcatcga tctcaagaat gaagccctca acatccgcag ccgcctcaaa
     9421 atggaaaagg ccaccctcca caagcagaag ctcatcaaca agctaaagat agtggatgcc
     9481 ttcatcaaga gcggcaacaa gccggaatac atgatcatgg aagccctgcc ggtgctgcca
     9541 cccacgctgc gtcctctggt cccgctggaa ggcggacgtt ttgccacggc cgatttcaac
     9601 gatctctatc gccgcgtcat cacccgcaac aaccgtctca agcagcttct ggagatccgc
     9661 gcccccgagg tgatcctccg caacgagaaa cgcatgctgc aggaagcggt ggacgccctg
     9721 atcgacaact cccgcaagcc ccgtcccgtg aggggaagag gcaatcgccc cctgaagtcg
     9781 ctgaccgacc agctcaaggg caagcaaggc cgtttccggc agaatctgct gggtaagcgc
     9841 gtggactatt ccggccgctc ggtgatcacc gtgggcccgg agctcaagct ctatcaatgc
     9901 ggcatcccca aggacatggc ggtggaactc ttcaagcctt atctgatcga acgcctgcag
     9961 aagctgggcg aggtagacaa ggtgaaaaac gccaagaagc tgatcgagaa gaaacagccg
    10021 gagatctggt ccattctgga agacgtgatc aaagattacc ccgtgctttt gaaccgtgct
    10081 cccactttgc accgcttggg catccaggca tttatgccgg tgctcacgga caacaaggcc
    10141 atccagttgc atcccatggt ctgtgtgcct ttcaacgcgg actttgacgg cgaccagatg
    10201 ggcgtgtatg tgcccctggg cgtggaagcg caaatcgaag ccagggtgtt gatgctctcc
    10261 acccgcaacc tgctcttgcc cgcgaacggc cgtctggcca tggcggcgaa ccaggatatc
    10321 gtgttgggct gttattatct caccatggaa gctggagacg ctccagccga caccaccagc
    10381 cttcgccatt tctacggacc cgtggaagta atcaccgctt atgaggcgga agagcagctt
    10441 tgcagcgtca aaggccacag cgaggtagaa cgcaatctgg atctgcacag ctggatccgc
    10501 gtccggatcg gcagtgaatt gattacaacc acggtgggcc gcgtgatctt caatcagatc
    10561 atcccccagg aagtgggcta tcaaaatatc acctacgaca aaggcaagct caacgacctg
    10621 gccatgctga gctatgacgc ggtgggtcag tggcgtaccg cggtgatgct ggacgacctc
    10681 aaggaactgg gattccgcta tgccacccgg gcgggagtga ccttcagctt tggagacatc
    10741 gtggtgccgg cccgcaaggg tgagatcatc gcgcagtctg aagccgaagt gaagaaggtg
    10801 tttgacctcc accagaaagg tggcatcacg gaaaacgaac gcgtaggccg catcgtggac
    10861 ctgtggaaaa agaccaccgt ccgcgtgacg gacgagatga tggaagagct cacgcatagc
    10921 cgtcagggcc tcaattccat ttatatgatg tataaatccg gagcgcgcgg ttccaaagac
    10981 cagatcaagc agttgggtgg gatgcgcggt ctgatggaca agccctccaa gatcgggtcc
    11041 accggtggcg ccgatgtgat cgagacgccc atcaaatcaa acttttatga aggcctcacg
    11101 gtgctggagt atttcgtatc cacccacggg gcccgtaagg gcctggcgga cacggccctc
    11161 aagaccgccg acgccggcta tctcacccgc agattggtgg acgtggcgca aaacgccatc
    11221 atcactctgg aagattgcgg caccggccgg ggcgtgcaca tgtctgtcct caaagaaggc
    11281 aacgagatcg tgcaatctct ggcggagcgg attcaggggc gggtatctct ggaggacgtt
    11341 gtggaccccg tcaccgggaa ggtgctcgtc aatgccgggg atgagatcag caacaagatg
    11401 gcgcgcacca ttcagaacca cggcattatc agcgtctatg tgcgctcggt gctcacctgt
    11461 gaagcggagc ggggcatctg cgccaagtgt tatggccgca acctggccac gcaaaagccg
    11521 gccacgattg gagaccccat gggcatcata gcggcgcaga gcataggcga gccggggacg
    11581 cagctcactc tgcgcacctt ccatatcggt ggagcggcct ccacggccac ggaactggcg
    11641 gaagtggtgt ccaaccacga cgggatcgtc aagtttgacc gcatgaacac cgtcaccaac
    11701 atcgaaggtc atctgatttc cgtgtctcac ctgggcaaga tcatcctggt ggaagcggag
    11761 gacgagacca gggtgctgga agaatacaag gtggaatacg ccgccacggt gcacgtgcag
    11821 gatggtcaaa agatcgtgac caataccagg ctgctgagtt gggaccaatt caacaacccg
    11881 ctgatttcga cggccaaagg cgtgttgcac tatgacaact ttatcaaaga catcaccttc
    11941 aaagaggaat acaacgatat cacctacagc cgcgatctca ccatcatcga atccaaggan
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng atattgacgg ccgcgtcacc
    12121 atcggcggtc tcaagaagac cgggcgcgac atctttgtga cgccttccaa cggtctcttc
    12181 gctcccgcgg atggaaaagt gctggtgcag agcgatgaac tgcgtaaccg ggtggtgctg
    12241 ctcaccgata aggccatcct ggcagccaac gatggcaagg tgagcatcca tgaggatagc
    12301 aaatcccgaa cggtgacggt gaccaaacgc ggcggcaagg gtgaggagta taagatcccc
    12361 aggtctttcc agatcattgt ggcgccggat cagtctgtga agaccggagc accgctggga
    12421 ggcctcgtct ttgatatccc ggagctgcag agccccattg tggagaccgg agacgcggtg
    12481 caaaagggcc aggccctggc cgggcgcaag tattccatcc cctccggcaa aaggatcatc
    12541 gtgcatcagg gcgactatgt ggaatccgga gacgcgcttt cggacggtcc tctggaccct
    12601 cacgacatgc tggtgaaagg gatcatcccg gcccagctca tgatcctgaa cgagatccag
    12661 gaaatctatc gtaaacaggg cgtgaagata gacgacaagc acgtgagcgt gatcatccgt
    12721 cagatgttta agaagctgcg gatcaccgat cccggcagca cgtctttcct ggaaggggac
    12781 atcgtggaca agacagcggt ggagcgtgaa aaccgggaag tgcttcaata tggcaaggcc
    12841 ccggcccaga gcgaacaatt gttgctgggg atcaccaaga cctccctgct cacccagagc
    12901 tggctttctt ccgcctcttt ccaggagacc accaaggtgc tcacccgcgc cgccatcgaa
    12961 ggcagcgtgg atcacctcga gggcctcaag gaaagcatca tcctgggtca ccgtatccct
    13021 gtgggaaccg gcaccaggat ctataatcag aggatcgaac aagctgtggg cgaaggtaag
    13081 acggtagccc agatcatcaa tagtttcgcc catccggata ctgagccgga aagcgaagac
    13141 ataatggatt tctaacccca atcaaagcca tgaaaataaa actggaggta atcagtgcca
    13201 accattaatc aactgattag aaaaggcaga actgaggcgc ttatgggatg tccgcaaagg
    13261 cgcggagtat gcactcgtgt ttacacgacg acgcccaaaa aaccgaactc agctcttcgt
    13321 aaagtagccc gtgtccgtct cgtgaacgga tttgaagtta cagcttacat tggcggtgaa
    13381 ggccacaacc tgcaggaaca cagcattgtg ctggttcgcg gcggtcgtgt gaaagacctt
    13441 cccggcgtgc gctatcacat cgtgcgcggc gctttggatt ctgctggagt ggaaaagcgt
    13501 gtaaactccc gctccaagta tggcaccaag cgtcccaagg tcaaaaaata gggaggaatg
    13561 aaagatgccg agaaagagaa aagccgtcgt ccgtgaagtc ctgccggatc ccaaatacaa
    13621 cgatgtcgtt cttaccaagt ttatgaactg cctgatggtg aagggcaaaa agagcctggc
    13681 tgaaaagatc gtctatggcg cttttgatat tattgccgcc aagaccaaac agccccccct
    13741 cgaagtcttc aaggccgctc tggataacgt ccgtcccctc gtgaaagtgg tatcccgccg
    13801 cgtcggtggt tccaactatc agatccccat ggagatcaac gagaaaaacg gccgcgctct
    13861 ggccttccgc tggctgatca gctatgcccg taaccgcagc gaaaagacca tgatcgaaaa
    13921 gctggccggc gaactgatgt ccgcctacaa aaaagaaggc tcctcgatca agaaacgtga
    13981 agacacccac aagatggccg aagccaacaa ggcctacgcc catctgagat ggtagtttaa
    14041 acaaagccta acaaatcgct tgtcgcccca gtcactatgg ctggggcgat tcctttgggt
    14101 taagcgtaac gcgttacgcg tgaagcggtt gaccctgcgg ctatcccagc agcgatgcag
    14161 agaagttgtt tcactacaga gatacggagg tccttttcac cacagaggcc acagaggcca
    14221 cagaggagaa agacagagag gattacaggg gttgtgggtt ttcgaattat aaagcagcta
    14281 agtagtggcg ggacagatca ctcttccaaa attcaaaacc acccctccag tcacatcgcg
    14341 cagcgatctc accttacaca ggtatctcac atgcgcagca tctcacagcg cagctctcac
    14401 atggcatagg catctcactc tttgtcttga cagaaagccc cctcccaaaa gaaatgcgct
    14461 caagcgcgtc cctgtagctc agctggatag agtggcaccc tccgaaggtg aaggtcag
//
LOCUS       DAOSFN010000054        14359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3482_length_14359_cov_10.108361, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000054 DAOSFN010000000
VERSION     DAOSFN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14359
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3482_length_14359_cov_10.108361"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(293..1783)
                     /gene="brxL"
                     /locus_tag="PLO35_04960"
     CDS             complement(293..1783)
                     /gene="brxL"
                     /locus_tag="PLO35_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HOH79049.1"
                     /translation="MESKIPSSQLPVVDLDDKIKRVFPLESVLKDPASQAIFAGRNLP
                     SYIKDWLIKCFTRFDEFDAEACAEFMNEKFPPRGPGFRRRLLQSSGPIQIFSRLIINT
                     DLMRGQMNFAIPDLGISSREGVVSMWMEQEKLERLHEGEVWGVMTLLYVRPGDNSAGY
                     VELIDFRPFEPYRVELSYYLEAREQFGIHEWIDLLIRSMEVNPDYRGPDGKLEFTLNK
                     KLTMISRLLVHVEPNLNLIELAPKGTGKSYLFNNLSKYGWTVSGGFVSRAGIFYNLST
                     RTPGIINNYDFLALDEVETLKFANDQDILGAFKNYLENGRVVVGHYKNVSDCGLMLLG
                     NIALDRKLKPRYEDFFRHLPEFFHSSALVDRIHGFIPGWVLFRFTEDLKLRGYALNTE
                     YFSEVLHLLRVEPSYSQLVKEALDIPRHADTRDTKAVIKLCTGMLKLLYPHACSLEDI
                     PRGEFEQLILAPALRARGIIRQQLSVLDPEFNPELPDITISQTYPV"
     gene            complement(1790..3085)
                     /locus_tag="PLO35_04965"
     CDS             complement(1790..3085)
                     /locus_tag="PLO35_04965"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HOH79050.1"
                     /translation="MEQNHIDKQSSIMVLEAIRAGIPSRELVGQLPDLRQKQLSTIDE
                     DLQSLEAGKAVKGRVLWGEYGQGKTHFLKQVEKHILAKGYAVSYISLSPDLGLNNFTS
                     FFPALASRVITQDAQIPGLLNKLLDYRFPPQKWDELLDSKDKLSHPLPLLIFESFQNH
                     DSREMIVLYNSLMGKKENLTFAKHLVKQDRKYEFQAMPKFLLRDHYKAFMEFLPLLIQ
                     ALGYKGWVLLIDELEIIGRMGRVSRLNSYRNLSWLLRLGSEHRLPVYTLAASAKSLQE
                     EVFWGVKKHDANDMPRLAMERGDEATYKLLLDFFSQITDDRGLVLKQESVAKLTPLLD
                     ALLEVHQIAIPWRHEPPNRMVIDFLKRVDPRMKPIRQTIRMFIETLDIYSVYGEVPGK
                     FKEKLMELYDFDDTIGENGDDDPPARGFEETPLRDMFES"
     gene            complement(3087..4274)
                     /locus_tag="PLO35_04970"
     CDS             complement(3087..4274)
                     /locus_tag="PLO35_04970"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HOH79051.1"
                     /translation="MNKPSQSDLELLREGNPPRDPELLRHLTVNNEWLQVMKDYCLDQ
                     VVAAGGSKVKILFGSSGTGKSHFINDIRVSAEERGFYAISIDLARTGYRLTDAVALYR
                     ALAADLDLDALEQALGALLLKHLGYEPSQWEEHGGSLSDFLCEYEGADLPEARKHIRT
                     CIHEIVNPLELDFAFRKFLHSFMEAVAERDKDFKDIAKAWLRGEKVERTNKSATRLYE
                     TLARHNARGWTYSLTEIIKLMGYKGVLLLIDQFEAILPQSEALVHYTPLKRKDIYEFL
                     RQLIDDLDFFKNILVIVCGDKTIIEDEKNGLQSYHALWMRIQPGFEQQPRLNVYADLI
                     DADLIYRDLISASDLESLRVKLRELGVWDQDGEAKAKTQTADTYANFRQFVTMSGTGF
                     WGG"
     gene            complement(4267..5028)
                     /locus_tag="PLO35_04975"
     CDS             complement(4267..5028)
                     /locus_tag="PLO35_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79052.1"
                     /translation="MQKHKDLDKLHKHYSSLLTASKNHNILLGEASAQGILDLEAGFW
                     ERVNTYLEAVKTEINGLKELYTDSDDYTARLAELLTARSIPLEREGSSIDIGPINISA
                     NVEDYFIQIKIGRKKQQITDLEPTKVVKLIETLYKRLNSSFNASAFFKRLQKAYDFAN
                     TRMYACREPKYGYSVALKDLFDLFALSPAAADYKLENFLWDLGRLYSYGASYDKYRIE
                     LGFSRDVRKMYLIRTASGDTIKASTLTIHLIAEDE"
     gene            complement(5207..6343)
                     /locus_tag="PLO35_04980"
     CDS             complement(5207..6343)
                     /locus_tag="PLO35_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002703292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79053.1"
                     /translation="MKDKQLHIKRWIEDDIIRHIHRGKAIIILGPRQCGKTTLLRSLS
                     SRSMDVLTLNCDEINDRQALENQSIKELTAVVGKAKVVLIDEAQRVQNIGISLKILVD
                     NLPGLQIIATGSSSFELSNRINEPLTGRKYEYHLHPFSQQELAAHFGNWDEHKDLTRR
                     LVYGAYPDIVRHPEEAEDLLRTLVGSYLFKDVFIYQDLRKPELLTRLLKALALQIGSE
                     VSYTELAQITMTDHQTVQRYMHLLENAFIIFRLYNYSSNQRNEIKNSRKVYFWDNGVR
                     NSLASDFRSCDNRPDIGKLWENYLVSERLKYLMNNRIHRSSHFWRNVNQSEIDYLEAY
                     EDKLDAWEIKWNPKRRISSLAFRNQYPKAVVSRVDRDNYREFIS"
     assembly_gap    6669..6768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7028..8725)
                     /locus_tag="PLO35_04985"
     CDS             complement(7028..8725)
                     /locus_tag="PLO35_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH79054.1"
                     /translation="MIKPRSIMPLLMLAAWLLLILVPAMASNATKPAPKRYMGIDVTD
                     LTLASQRRLGLESTEGVLVTLVVQGSPADSCGLRVDDLIVSIDAVPIPNPKAFRDVLS
                     KYQPGDQARIKIIRDQTHLAFDLTFGVMPARPEGGRLSWSDSKAQLIGDLLDTIDIHN
                     QSMGSLAIADSQQIIFARALGYKYLNGDTRLTADPSTRYRIGSITKTFTAVMIFQLLE
                     EGRLSLDTRLVDFFPTVPNADSISIAMMLTHQSGIFSFTDIPNYLRWSLQPRTRDEVL
                     SRICSFPAAFNPGERHIYSNSNYLLLGYIIEALDGRDYAASLNTRITGKIGLDDTYYG
                     GDIDVSNNEAMSYLYMGYWEPGRMMNMDIPGGAGGIVSTASDLLRFVAALFKDKLISP
                     QSLKVMLECEEGFGSGIFNMPMGTHSYYGHTGSIDNFRSILLYSPEDGYSLCWLSNGE
                     ADPSLGLIGLISAILSDQHVNIPQYVKAEQLVELSTLVGNYHNEELAMQIEIMAIDGE
                     IYAQAADQPAIKLKAIDGRRFECPSVGLVIRFDPEGRSFILEQDEATLLFSREISTD"
     gene            complement(8885..9817)
                     /locus_tag="PLO35_04990"
     CDS             complement(8885..9817)
                     /locus_tag="PLO35_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79055.1"
                     /translation="MVLLRLKILLLAAVLTLCSVAWGQYRFIANSRYVSIYGTKETPA
                     STAELRAMLDAGIDLIQMETGVYLDDGPEIFIVPDRESYQRLSLGRDKVVEFSDAFYS
                     SIEERVYIHSREQIGQDFVKVLIHEYTHWFLDHLFDGTTLWFHEGLATLYANQLSVES
                     YLYFTQQRFFGRQSDLFQMSWEYPTQPSEWQNYYLTSYFAIRYLKDKKEQGWRRFWDA
                     VAAGRKEGHRLMFTRVFNASFNTTLLDFNAEFRAHCQGLAWQYLILGFNSILLACLPF
                     LLFFVARKRRRKLRSLPDLPLPVETEDEDKPSGD"
     gene            complement(9817..10080)
                     /locus_tag="PLO35_04995"
     CDS             complement(9817..10080)
                     /locus_tag="PLO35_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOH79056.1"
                     /translation="MRIDLLLNKLCLTKTRGIAKTACDKGLVSINGKPAKASATVNAM
                     DVIELKLYGFLHELRVDRLPEGNVAKKDAASYYSLLKREPIAD"
     gene            complement(10055..12967)
                     /locus_tag="PLO35_05000"
     CDS             complement(10055..12967)
                     /locus_tag="PLO35_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOH79057.1"
                     /translation="MYHNPTAFYKMLFEQSPVSIWVEDFTGVVEFIERHKELGVKDFR
                     AYFMTYPEKLLECVSQLKVVDVNDTTLQTYKARDKQTLVRGLPLIFRDEAIYCLMEST
                     IALSENKTHFDGQGINYDLQGNKLMFRISWNIPGPEMSDYKNVIVVMQDYTRLDQVRR
                     ELEESESLFRSIFEQSSEGLVLLDSQGSIRLVNQSFSSIIGAPQVDMAGKAIWDIYAK
                     FASENSLDRDLPLTADSLRVLLEHPEPEQQVYEYSFRDSRSKYHVHKLTIQPIQVNDQ
                     RLFTMVLTDLSAFNRSELVTTMLHRISHAVNAEVSLDELFHIIHESMGRLLDVSNFYI
                     ALYDSKSRLITFPYVVDEFEDDSPISIDFADSLTAQIISGAQTLLLEEEEVHQRTGLD
                     YGDSRLAKNYLGAPLILSGNVIGALAVQSYTLTNLYTEEDRLFLESISEQIAFALHKK
                     QSDENITVLIQAIEQAGEGIAIFSAQGFIYYVNTVFEKSIGYARAELLDKPLEFLPFD
                     RNSRKEMQTSWMRVRSSQPWRGKVDMIRKDGSKITLDMVVKPVIDHEGHLSSIIASCK
                     DVTYEILREEQLKRTQRLEAIGRLTGGIAHDFNNVLSAVIGYTELAAEDLDPYSDSAQ
                     KLVEVLKSANRAKEMIAHLISFSRQEEARTELIELVDHVKESVRFLGSYLPKNIEIAE
                     SYQVPNSTVMAVPGQVHQIVINLGTNAMHAIGQKQGHLEISVDQISFLSKDMVAFPEL
                     DQCEYLRIIVRDNGCGIEENLIQHIFDPYFTTKRSTEGTGLGLSIVHSIVQAHRGAIR
                     VESTVGVGTAFCIYLPVYKADRSIKPVEETIIDEEVSGVENIMFVDDEPMLVNVFKQG
                     LSRLGFNVEGFTDPRKALEYFDRNHHRVDMVLTDTTMPYMNGVDLAEKMLAIDPRMPI
                     IICTGFTTLISAEGAKQLGIRDFVMKPFKIKEIAQLIRDILDKEEKINDADRFAPE"
     gene            complement(12967..14169)
                     /locus_tag="PLO35_05005"
     CDS             complement(12967..14169)
                     /locus_tag="PLO35_05005"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOH79058.1"
                     /translation="MNILVLNCGSSSIKYQFINMKDQNVMAEGIAERIGEDIALFTYK
                     SPAYTKKKREMVIEDHEQGLQLIIDSLLDPAYGVIGSVREIDAVGHRLVHAGEHYSDA
                     VIVTDHVIDVMRECISLAPLHNPANLKGIEAVKKNMPDVPQCGLFDTAFHQTMPSQAY
                     MYPLPLEFYHTHKIRRYGFHGTSHKYVSMKAAEYLGRDLASLKIISCHLGNGASITAI
                     NGGKSVDTSMGLTPLEGLMMGTRCGDLDPAIPIHMQTQLGLSVDEVNSILNKKSGMLG
                     LSHISNDMREIEDEILLHSNPKAIQAHDVYCYRIKKYIGAYFAAMNGLDVLIFTGGVG
                     ERMPIMRAQVCADMEALGITLDPEPNALFNDSIEVLNRPEGKVSVLKVPTNEELMIAL
                     ETQRLLTK"
BASE COUNT         3256 a         3600 c         3785 g         3618 t
ORIGIN      
        1 ccggaagagt cctgggctct ggaatctacc tctacaaact ggaggccgac ggctatagca
       61 agttctataa gatgatcatg atgaagtagt tgtcatcgta tattgagagg gtggaaacac
      121 cttcgcaaac ggtcgcggca cagactacag ctctgccgcg accggttttt atcaggagtg
      181 tcgaatttta gcccagcccg tgaacgaccc tcttatatgc ctgctctact atcttccata
      241 gatgagcccc tcagtgtatc tatgcagaac ccgccttata gctaacgcgc agtcagaccg
      301 gatatgtttg gctgatagtg atgtccggca gctcagggtt aaactcagga tccaggacgc
      361 taagttgctg acggataatg cccctggccc tcaaagccgg tgccaggatc agctgctcaa
      421 actcccccct tggaatgtct tccaggctgc aggcatgcgg atagagcagt ttgagcattc
      481 cagtgcagag cttgatcaca gctttggtat cacgcgtgtc ggcatggcgc ggaatatcca
      541 gggcttcctt aaccagttgg gaataagatg gttccaccct tagcaggtgc agcacctctg
      601 aaaagtattc ggtattgagc gcatagccac gcagcttgag gtcttcggta aagcggaaca
      661 gcacccagcc ggggatgaaa ccgtgaatgc ggtccaccag ggctgaggaa tggaaaaact
      721 cgggcagatg gcgaaagaaa tcctcatatc tgggcttgag tttgcggtcc agagcgatat
      781 tgcccaatag catcagaccg cagtccgaaa cgtttttata gtggcccacc accaccctgc
      841 cgttttccag ataattcttg aaagcgccca ggatgtcctg atcgttggca aacttgagcg
      901 tctccacctc gtcgagggcc aggaagtcgt agttgttgat aatcccggga gttctggtgc
      961 tgaggttgta gaaaatcccc gcccgggaga cgaagccgcc gctcacggtc cagccatact
     1021 tggataggtt gttgaagagg taggatttgc ctgttccttt gggggccagc tcgatcagat
     1081 tgagattagg ctccacgtgc accagcaggc gcgagatcat ggtaagcttt ttgttgagag
     1141 tgaattccag cttgccatcc ggcccccggt agtcaggatt tacctccata ctgcggatca
     1201 gcaggtcgat ccactcatgg atgccaaact gctccctggc ctccagataa taactcagtt
     1261 ccacccggta aggctcaaag ggacggaagt caatcagttc cacatatccg gcgctgttat
     1321 cacctgggcg cacatacagc agggtcatca ctccccagac ctcaccttcg tgcagacgtt
     1381 ccagcttctc ctgctccatc cacatggaca cgacgccttc ccgcgagctg atccccagat
     1441 caggaatggc aaagttcatc tggcccctca tcagatcggt gttgatgatg aggcgtgaga
     1501 atatctggat aggaccgctg ctctgcagga gcctgcgtct aaaacccgga ccgcggggag
     1561 ggaacttctc attcatgaat tccgcgcagg cttccgcgtc aaattcatca aaacgggtga
     1621 agcacttgat cagccagtcc ttgatatagg aaggcaggtt gcgtcccgcg aagatggcct
     1681 gggaagctgg gtccttgagg acggattcca gggggaagac gcgcttgatc ttgtcatcca
     1741 ggtccacgac gggaagttgg gagctgggga ttttactttc cactatcgtt tatgattcaa
     1801 acatatcccg cagaggagtt tcctcaaatc ctctggccgg gggatcatca tctccgttct
     1861 caccgatcgt atcgtcaaaa tcatatagtt ccatcagttt ctctttgaat tttccaggga
     1921 cctctccata tacagagtag atatcgaggg tctcgataaa cattctaatc gtctgacgga
     1981 taggcttcat gcgtgggtcc acccgcttga ggaagtcgat caccatgcgg ttcggcggtt
     2041 catgccgcca gggaatggcg atttgatgta cctcgaggag ggcatctaaa aggggtgtga
     2101 gtttggcgac gctttcctgc ttgaggacca atcctctgtc atcggtaatc tggctgaaaa
     2161 aatccaacaa cagcttgtag gtagcttcat ctccgcgctc catggccagg cggggcatat
     2221 cgttcgcgtc gtgcttcttg actccccaaa acacctcttc ttgtaaggat ttagctgacg
     2281 cggccagggt gtagaccggc agccggtgtt cagaccccag cctgagcagc caggataggt
     2341 tgcggtagga attcaggcgg ctcactctgc ccatgcggcc aattatctca agttcatcga
     2401 tgagcagcac ccagcctttg tagcccaggg cctgtatcag caagggtaag aactccataa
     2461 atgccttgta atggtccctc agcaaaaact ttggcatggc ctgaaactca tatttccggt
     2521 cttgcttgac cagatgtttg gcaaaggtga gattctcttt tttgcccatc aggctgttgt
     2581 aaagaacgat catttccctg gaatcgtggt tttgaaagct ctcgaagatc agaaggggga
     2641 ggggatggct cagcttatcc ttactatcca gcagttcatc ccatttctga ggaggaaagc
     2701 gataatcgag gagcttattg agcagaccgg ggatctgagc gtcctgggtg ataactctcg
     2761 aggccagagc agggaagaag gatgtgaagt tgtttaaacc cagatcaggg ctcaaactga
     2821 tgtagctcac ggcgtaaccc ttggccagga tatgcttttc cacctgtttg agaaagtggg
     2881 tcttgccctg tccgtattcg ccccacagca ctctgccctt gaccgccttt ccagcttcca
     2941 ggctttggag gtcttcgtct atggtgctaa gctgcttctg tctgagatct ggcaactgcc
     3001 ctaccagttc ccgggatgga attcccgccc gaatggcttc caacaccatg atgctgcttt
     3061 gtttgtcgat atggttctgt tccatgtcac cctccccaga atccggtccc ggacatcgta
     3121 acgaactgtc tgaaattggc ataagtgtct gccgtctgcg tctttgcttt tgcttcgcca
     3181 tcttgatccc agacacccag ttccctgagt ttaaccctca aagattccag atcgcttgcg
     3241 ctgatcaagt cccggtagat gagatcggcg tcaatcaggt cggcatagac attcaagcgg
     3301 ggttgttgct cgaacccggg ttgaatcctc atccagaggg cgtgatagct ttgcagtccg
     3361 tttttctcgt cttcgatgat agtcttatct ccgcacacga tcaccaggat gtttttgaag
     3421 aaatccagat cgtctatcag ctgccgcaaa aactcataga tatccttgcg tttgagaggc
     3481 gtataatgca ccagcgcttc cgattggggc aaaatggcct caaattggtc gatgagcagc
     3541 aacactcctt tataccccat cagcttgata atctcagtaa gggagtaagt ccagccgcgg
     3601 gcattatgac gggcaagcgt ttcgtagagg cgggtggcgg acttattggt gcgctcaacc
     3661 ttttctcccc gcagccacgc tttggcgatg tccttgaagt ctttatccct ttccgccact
     3721 gcctccataa aagagtggag gaattttcta aaggcaaagt ccagttcgag ggggttgaca
     3781 atctcgtgga tacaggtcct gatatgtttg cgggcttctg gaagatcagc tccttcatat
     3841 tcgcacagga agtctgataa gctgccgcca tgctcttccc attgactggg ttcatagcca
     3901 agatgcttca acaacagagc cccgagggct tgttccagtg cgtccagatc aagatcggct
     3961 gccaaggcac gatacaaggc cacggcatcg gtcagtctgt atccggtcct ggccaggtcg
     4021 atgctgatcg cgtaaaatcc ccgctcttca gctgatacgc ggatatcgtt gatgaaatgg
     4081 ctcttgccgg taccgctgct gccaaagagg attttcacct tgctgcctcc agccgcgacc
     4141 acttggtcca ggcagtaatc cttcatcact tgcagccatt cgttgttgac ggtcagatgc
     4201 ctcaagagct ccgggtcacg gggaggattg ccctcccgca gtaactccag atcggactgg
     4261 ctgggcttat tcatcttcgg cgatgagatg gattgtgagc gtggaagctt taatggtatc
     4321 acctgaagcg gtacggatca gatacatctt ccggacgtct ctgctaaaac caagctcgat
     4381 cctgtattta tcatagctag ccccgtagct gtaaaggcgc ccgaggtccc agaggaagtt
     4441 ttccagttta taatctgccg cggccggaga aagagcaaag agatcaaaca gatctttgag
     4501 ggcaacgctg tagccgtact tgggttcgcg gcaggcatac atgcgggtat tggcaaagtc
     4561 gtaagccttt tgaaggcgtt taaagaacgc ggaggcattg aaggaactgt tgagtctttt
     4621 ataaagggtc tctatcagtt tgaccacttt ggttggctcc aaatctgtga tttgctgttt
     4681 cttgcgtccg atcttgattt ggataaagta gtcttccaca ttcgcgctga tgtttatggg
     4741 gcctatatcg atactgctgc cttcccgctc cagagggatc gaccgggcag ttagaagctc
     4801 ggccagtctg gcagtgtagt catcgctgtc ggtgtagagt tccttcaagc cgttgatttc
     4861 ggtttttact gcttccagat aggtgtttac ccgctcccaa aaacctgctt caagatccag
     4921 aattccctgg gcagaggctt cacccaggag gatgttgtgg ttttttgagg ctgtgagcaa
     4981 agagctgtag tgtttgtgaa gcttgtccag atccttgtgc ttttgcatat ctaactcctg
     5041 cagaagattt tgctctgtgc cccatatccg tctgaggggc aagagtgtta aactatttta
     5101 cctgttgccg tgtggcgcga aaaacgtcaa ggggaaaatc cgtctcttac ggggaaatgt
     5161 tcccggcagg gacctcaatc ccggaacggg agatttatcg cacgcgttac gagataaatt
     5221 cccgatagtt atctctatcc actctggaaa ccacggcttt gggatactga ttgcggaaag
     5281 caagcgagga tatcctccgc ttgggattcc atttgatctc ccaggcatcc agcttgtcct
     5341 cataggcttc cagataatct atctcgcttt ggttgacgtt acgccagaag tggctggatc
     5401 gatggatgcg attgttcatc agatatttaa gtctctcgga tacgaggtag ttttcccaca
     5461 acttacctat atctggcctg ttgtcacagg agcggaaatc cgaggccagg ctgtttcgca
     5521 ctccattatc ccagaaatag accttgcggc tgtttttgat ctcgttacgc tggttagagc
     5581 tgtagttgta gaggcggaag atgataaacg cgttttccaa aaggtgcata taacgttgca
     5641 cggtctgatg gtctgtcatg gtgatctggg ccagttccgt gtatgatact tccgagccga
     5701 tctggagggc cagggctttt agaagacggg tcaggagttc tggtttgcgg aggtcctggt
     5761 agatgaatac gtctttgaag agatagctgc ctacaagcgt tctgagtagg tcttcggctt
     5821 cttcaggatg tctgacaata tcaggataag cgccataaac cagtcgtcgg gtcagatcct
     5881 tatgctcgtc ccagtttcca aaatgagcag caagttcttg ctgtgaaaaa ggatgcaggt
     5941 gatactcgta ttttcttccg gtcagaggtt cgttgattcg gttgcttagt tcgaacgaag
     6001 aagaaccggt agctatgatc tgaagccctg gcaggttatc caccaggatc ttaaggctga
     6061 ttccgatgtt ctgcacccgc tgcgcttcat cgatcagcac cacttttgcc ttgcccacta
     6121 cagccgtaag ctctttaatt gattgatttt cgagagcctg acggtcattt atctcgtcac
     6181 aattcaacgt caatacatcc atgctgcgag acgatagaga cctgagcagg gtggtctttc
     6241 cgcattgacg cggtcccaga atgataatag ccttgcctct gtggatgtgc cggattatgt
     6301 cgtcttcaat ccagcgctta atatgaagct gcttgtcttt cataaacatc acctttatga
     6361 taatgaatcc atcggacagt atgagataaa tctgtcaagc aaaataaccc atgattacga
     6421 gattatattc gtgcaataag ggccctatta cgagattatg ttcttgtgat aggggatgtc
     6481 tatattatcg ggatggacct tgaacacctc aatgagctgc caaatcatac gagattaaaa
     6541 gcgctgagag cttagttctc cggctgtaat actcatgaaa gagtagatag ctcttcttga
     6601 ccaaaaagtt catgccttct catgaagtcc aatagttgac gacttgaatt cacttgggta
     6661 tccagacann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg tacataagcc
     6781 agcggatgcg ccagtagata ctctcggttg tgggagttgg catccctaaa tctataatcg
     6841 tgtaaacata ggtatctact gacgattgat tggcattgat gtgccaaagt caattcgtca
     6901 cttcataact cacctcttaa cagtgtgttg caagtaggca ctgtcgaact tgattcaaac
     6961 atatcccgca gcggagtttc cttaagctca ctggccgggg gatcatcatc tcagctctca
     7021 ccgaacctca atccgttgat atctccctgg agaacagcag cgtcgcctca tcctgttcca
     7081 gaatgaatga ccggccctcg gggtcaaaac ggattaccag accgacgctg gggcattcaa
     7141 atctcctgcc gtctatggct ttaagcttga tggctggttg atccgcggcc tgggcataga
     7201 tttctccatc tattgccata atctcgatct gcatcgcgag ttcctcgttg tggtagttac
     7261 ccaccagagt ggaaagctca accagttgtt cagccttaac gtattgaggg atgtttacat
     7321 gctggtctga caggatggct gagatcagcc ctatcagtcc cagactggga tccgcttcgc
     7381 cgttggagag ccagcagagc gagtagccgt cttcaggact gtagagcagg atagatctga
     7441 agttgtcgat cgaccctgtg tgaccgtagt agctgtgagt tcccatcggc atgttaaaaa
     7501 tgccgctgcc aaagccttcc tcgcattcca gcatcacttt cagactctgt ggggagatga
     7561 gcttatcctt gaagagcgct gccacgaaac gcaatagatc agacgcggtg gagactatcc
     7621 cacctgctcc accgggaata tccatattca tcatccggcc cggttcccaa tatcccatat
     7681 agagataaga cattgcctca ttattgctta cgtcgatatc gccgccataa taggtgtcat
     7741 cgagtccgat cttgccggta atccttgtat tcagggacgc ggcataatcg cgaccgtcca
     7801 gagcttcgat gatgtatccc agcagcaaat agttggagtt gctgtaaata tgtctttcac
     7861 caggattaaa tgccgcggga aaggagcaaa tcctggagag gacctcatcc cgggtcctgg
     7921 gctgtaggct ccatctgagg taattgggta tgtcggtgaa agaaaatatc ccgctttgat
     7981 gagtgagcat catggcgata ctgatagaat cggcattggg aacggtggga aaaaagtcaa
     8041 ccagtctggt gtccagggat aatcgtccct cttccagcag ctgaaagatc atcacggcag
     8101 taaatgtctt ggtaatggaa ccgatacggt aacgggtgct ggggtcggca gttagcctgg
     8161 tgtcgccatt caagtattta taaccgagcg ctctggcaaa aatgatctgt tggctatcgg
     8221 cgatggccag gctacccatg ctctggttgt ggatgtctat tgtgtccagc aaatcgccga
     8281 ttagctgtgc tttggaatcc gaccagctga gcctgcctcc ttcggggcgg gccggcatga
     8341 caccaaaagt gagatcaaat gccagatgtg tttgatccct gatgatcttg atcctggcct
     8401 ggtcaccggg ctgatatttt gacaatacgt cccggaaagc tttcggattg gggatcggca
     8461 cggcatcgat actcacgatc agatcatcga cgcgtaatcc gcaactgtca gccggactgc
     8521 cttgcacaac cagggtaacc aatacccctt cggtcgattc cagccccagt ctgcgttggg
     8581 aagcaagagt aagatcggtg acatcaatac ccatatagcg tttgggggcg ggcttggttg
     8641 cgttgctggc catagcggga accaggatga gcagaagcca tgctgccagc ataagtagcg
     8701 gcattatgga tcgcggttta atcatgttaa ctccttcacc agttcctacg cgtcaatcct
     8761 ttcaagaacc gggactaatg cctggcctgc tcgattgtgg aatgtggaca taacctggcc
     8821 gggtaattcc aacaccgatc cagtcttttc agccagtaac aactcagaag gatatcctga
     8881 ggcatcaatc cccggagggc ttatcctcat cctcagtctc aaccggcaaa ggaagatctg
     8941 gcaaggaacg cagctttcgg cgccgttttc tggcgacaaa aaagagcagg aaaggcaaac
     9001 aggccagcag gatgctgttg aagcccagga tcagatactg ccaggccaga ccttgacaat
     9061 gagctctgaa ttcagcattg aaatccagca gagtggtgtt gaaactggcg ttgaagacgc
     9121 gggtgaacat cagcctgtga ccttctttcc ggccagccgc taccgcatcc caaaagcgtc
     9181 tccagccctg ctctttctta tccttgagat agcggatggc aaagtaggag gtgaggtagt
     9241 agttctgcca ttctgagggc tgggtaggat actcccaact catctgaaac agatcgctct
     9301 gacggccgaa aaagcgctgc tgggtgaagt acagatagct ctccacactg agttgattgg
     9361 catagagggt ggcaagccct tcgtggaacc agagggtggt cccatcgaat aggtggtcca
     9421 gaaaccagtg cgtgtattcg tggataagca ccttcacaaa gtcctggcca atctgttccc
     9481 tggaatggat gtaaacccgc tcttcgatgc tgctgtagaa ggcatcgctg aattccacca
     9541 ctttatctct gccaagactc agtctctgat agctttcccg gtcaggaacg atgaagatct
     9601 caggtccatc gtcaagatag acacccgtct ccatttgaat caggtctatg cccgcgtcca
     9661 gcattgctct cagttcggcg gtcgaggcgg gtgtttcctt tgtgccgtag atacttacat
     9721 aacgtgagtt ggcaataaaa cggtattgtc cccaggcgac ggaacaaaga gtgaggaccg
     9781 cggccagcag caaaatcttg agccgcagga gaaccatcag tccgctatgg gctcccgctt
     9841 gagcagcgag taatagctgg cggcgtcttt tttggccaca ttgccctcgg gaagcctgtc
     9901 cactctcagt tcatgtaaga acccatagag tttgagttcg attacgtcca tggcgttgac
     9961 cgtcgccgag gccttggcag gcttgccgtt gatgctcacc aggcctttat cgcaggcggt
    10021 tttggcgatg ccgcgggtct tggtgaggca gagcttattc aggagcaaat ctatccgcat
    10081 cgttgatctt ttcctctttg tcgagtatgt ctctgatcaa ttgggctatt tccttgattt
    10141 tgaagggttt catcacaaaa tcccggatgc caagctgttt ggccccttcg gccgagatca
    10201 gggtcgtgaa tccggtacag atgatgatgg gcatccgggg atcgatagcc agcatctttt
    10261 cggccaggtc caccccgttc atatagggca tggtggtgtc tgtgagcacc atatcgacac
    10321 ggtgatgatt gcggtcaaaa tactcgaggg ccttgcgggg gtccgtgaat ccctccacgt
    10381 taaatcccag cctggaaagc ccttgtttga acacgttgac cagcatgggc tcgtcatcca
    10441 caaacatgat gttttccacc ccgctgactt cttcatcgat gatggtctct tccacgggtt
    10501 tgatagatct atcagctttg tagacaggca aatagatgca gaaggcggtt cctacgccca
    10561 cggtcgattc cactctgata gcaccccgat gagcctgaac gatgctgtgg acgatggaca
    10621 agcccagacc ggttccttcc gtactgcgct tggtggtgaa ataggggtcg aagatatgct
    10681 ggatgaggtt ttcctcgatg ccgcagccgt tgtcgcgaac gatgatcctg aggtattcac
    10741 actgatcgag ctccggaaaa gccaccatat ccttgctgag gaaggaaatc tggtccacgc
    10801 tgatctcaag atggccctgt ttctgaccaa tggcgtgcat ggcgttggtg cccaggttga
    10861 tcacgatctg atgcacctgt cccggcacgg ccatgacggt gctgttgggc acctgatagc
    10921 tctcggcgat ctcaatgttt ttgggcagat aagagcccag aaaacggacc gattccttga
    10981 cgtggtctac cagttcgatc agctcggtgc gggcttcttc ctgacggctg aaggagatca
    11041 gatgagcgat catctctttg gcccggttgg cgctcttgag cacttccacc agtttttgag
    11101 cactgtcgct ataggggtcc agatcttcag cggccagctc ggtatatccg atcacggcac
    11161 tgaggacgtt gttaaagtca tgcgcgatgc cacccgtgag acgtccgatg gcttccagac
    11221 gctgagtgcg tttgagctgc tcttcccgca ggatctcata agtgacgtcc ttgcagctgg
    11281 ctatgatgct ggaaagatgg ccctcgtgat cgatcaccgg cttgactacc atgtccaggg
    11341 tgatcttgga gccgtccttg cggatcatat ccactttgcc ccgccagggt tgagaagatc
    11401 tcacccgcat ccaggaagtt tgcatttcct tgcggctgtt ccgatcgaag ggcagaaact
    11461 ccaaaggttt gtccagaagc tccgcgcggg catagcctat ggatttttca aacacggtgt
    11521 tcacgtagta gataaagccc tgggctgaga agatggcaat gccctcaccg gcctgttcga
    11581 tagcctggat gaggacggtg atgttttcgt cgctctgttt tttatgcagg gcaaaggcga
    11641 tctgctctga gatggattcc agaaagagcc tgtcttcctc ggtgtagagg ttggtgaggg
    11701 tgtagctctg cacggccagc gcgccgatca cgtttccgga caggatcagc ggcgcgccca
    11761 ggtagttttt ggccagacgg ctgtcaccat agtccagccc ggtgcgctga tgcacctctt
    11821 cctcttccag gagcagggtc tgagccccgg aaatgatctg tgcggtaagg gaatcggcaa
    11881 aatcgatact gatggggctg tcatcttcaa actcgtccac cacatagggg aaggtgatca
    11941 gccggctctt ggaatcataa agcgcgatat aaaagttgct tacatccagc aaccggccca
    12001 tggattcatg gatgatatga aacagctcgt cgaggctgac ttccgcgttc acggcatggc
    12061 tgatgcgatg cagcatggtg gtgaccagct ctgaacggtt gaaggccgaa agatcggtga
    12121 gcaccatcgt gaagaggcgc tgatcgttta cctggatggg ctggatagtg agcttgtgga
    12181 cgtgatactt gctgcgggag tccctgaagc tgtactcata gacttgctgt tctggttcgg
    12241 gatgctccag caggactctc aaagaatctg ccgtgagggg cagatcgcgg tccaggctgt
    12301 tttctgaggc aaatttggca tagatatccc agatcgcttt accggccatg tccacctggg
    12361 gagcaccgat gatactggaa aatgactgat ttaccagcct gatgctaccc tggctgtcca
    12421 acagcaccaa gccttctgat gactgctcaa agatgctgcg gaagagggat tcgctctctt
    12481 ccagctcgcg gcgcacctgg tccagacggg tgtaatcctg catcaccacg atcacattct
    12541 tgtaatcgct catctccggg cccggaatgt tccaggagat gcggaacatg agcttattgc
    12601 cctgcaggtc atagtttatg ccctgtccgt caaagtgagt cttgttttca ctgagggcaa
    12661 tggtggattc catcagacag tagatggctt catcgcgaaa aatgagcggc aggcctctca
    12721 cgagggtttg cttgtcccgg gctttatagg tctggagcgt ggtatcgttt acatccacta
    12781 ccttgagctg agagacgcat tccagcagtt tctccggata ggtcatgaag taggcccgaa
    12841 agtctttcac gcccagttct ttgtggcgtt cgataaactc aacgacgcct gtaaagtctt
    12901 ccacccagat ggagactggc gattgctcaa agagcatctt gtaaaacgcg gtgggattat
    12961 gatacattat ttggtaagca gcctttgggt ctcaagagcg atcatcagct cttcgttggt
    13021 gggtactttg agcacgctga ccttgccttc cgggcggttc agcacttcga tactatcgtt
    13081 gaaaagggca ttgggttccg ggtcgagagt gatcccgagg gcttccatgt ctgcacagac
    13141 ctgagctctc atgattggca tcctttcacc cacgccgcct gtgaagatca gcacgtccag
    13201 tccgttcatg gcggcaaaat aagccccaat atacttcttg atgcgatagc aatagacgtc
    13261 gtgggcctga atggccttgg gattggagtg cagcagaatc tcgtcctcga tctcacgcat
    13321 atcgttgctg atatgagaga gacccagcat gccgctcttc ttatttagaa tggagtttac
    13381 ctcgtccacg ctgaggccca gttgagtctg catgtgaatg ggaatggccg ggtccaggtc
    13441 tccgcagcgg gtgcccatca tcaagccttc cagaggagtg agacccatgg aggtgtccac
    13501 gcttttgcca ccattgatgg cagtgatgga agctccattg cccaaatggc aggagatgat
    13561 cttgaggctg gccagatcgc ggcccagata ctcggcggcc ttcatgctca catacttgtg
    13621 ggaagtgccg tgaaaaccgt aacgccggat cttgtgagtg tgataaaact ccagcgggag
    13681 gggatacata taagcctggg aaggcatggt ctgatggaaa gcggtgtcaa acaaaccgca
    13741 ctgaggcacg tccggcatgt tttttttgac tgcttcaatg cctttgaggt tggcagggtt
    13801 gtgcaagggg gccagggata tacactcccg catcacatcg atcacgtgat cggtgacaat
    13861 cacggcatcc gaataatgct ctccggcatg gacgagccgg tgccccaccg cgtcgatctc
    13921 acgcacgctg ccaatcactc cgtaagcggg gtccagcaga ctgtcgatga tgagctggag
    13981 gccctgttca tggtcctcga tcaccatttc ccgcttcttt ttggtataag ccgggctttt
    14041 ataggtaaac agcgcgatgt cttcgccgat ccgttcggcg attccttcgg ccatcacgtt
    14101 ctggtctttc atgttgatga actggtattt gatggaagag cttccgcaat tcagcaccag
    14161 tatattcatg ggattcctct ctttagactt ttcaggatta gtatcggcaa acctgtaagc
    14221 agattataac aatttttgtt ccatatttca cacagaataa gggcgtgagc tcaaatcaag
    14281 gggggatgat gtggaatttc gtggaaaaaa tggtgggtga tactggattc gaaccagtga
    14341 cctctacgat gtgaacgta
//
LOCUS       DAOSFN010000055        14289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3508_length_14289_cov_8.859562, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000055 DAOSFN010000000
VERSION     DAOSFN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 14289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14289
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3508_length_14289_cov_8.859562"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..627
                     /locus_tag="PLO35_05010"
     CDS             184..627
                     /locus_tag="PLO35_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79059.1"
                     /translation="MKVTLKHRLGIYQGECDDLVYYYNSKLKTMVARRYTKPRASQSN
                     ARLSSNSKHLKALNPSAAFIADLKLYQLLHNSELKAGAKLINNHYALYTRLMYAQARA
                     LDIPVTELSRALIESAPLPCRSVKTAVEGGLIEEVEGYDRLTGLI"
     gene            complement(775..3189)
                     /locus_tag="PLO35_05015"
     CDS             complement(775..3189)
                     /locus_tag="PLO35_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOH79060.1"
                     /translation="MQIKTLLLALSMLFLPGMALAEVLSVGSDGTQPYTSIQAAINNS
                     THGDIIQVSPGEYFEHLDTMGKNLTIRSLYIFTQDEETIHGTIIRASFPNSCLLVHNH
                     ETVIIDGFNLVNNMPEVLSHPHVNGWNLKAGGIEVYEDSSIQITNCVIRNCYGQGAGG
                     VFFVGDNFYMSNTKIYECYGVDGPGGLAIGGTGLVSIVFDAVLPNSIYNNTSFNGMDI
                     LLADLPQPLNLVLDTFSVILSEPDWFYIQYDGAQDLSISVQHAYFDLVNHDLYVAPGG
                     DDGNSGLSPQDPLKTIARAAKIIASDSLAAKTIHLAAGEYNLTGSGQFFPLTLKSHTR
                     LRGDDPELVIFNPENSGRGCLSFFNQHNIRIENITFIPHVIETANTFPIFAARVHNLE
                     LRNLSWNGNIEDYFDDFTRIRIGYCDGVICENITARNATTNDHNNLAVSVLSSRNIFL
                     NNILIDNLVILSDGFHSGLLVYESDATVRNTVISNCYANCGTMFMYQSRVTEGVNLDL
                     SNMLFINNISTSADAGAPIYIFNQFERAQIRNSTFAYNNAWGRYVMLLRGYGDIYNCI
                     FYNPDNHLRDLLLNNVNDGDTFNPGVSYSLFTVPVGVQDSTAVSISNLLIGEDPLFEG
                     HGQPAWDTSLPASYQLSSISPCVNAGTPDVSGLNLPPTDLAGNLRIWDGRVDMGCFEY
                     GSVPYVSNPEPDIPSAPDQIVLSLFPNPVRATASRAAYVFIEFTLPKLAKEDAIVQIY
                     NLKGQRLRTLRPGIVPASTSGQGGIYSTVYDCRDESGRKLPSGIYLIRVESGKYHSTG
                     RFTIVK"
     gene            3724..4323
                     /locus_tag="PLO35_05020"
     CDS             3724..4323
                     /locus_tag="PLO35_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79061.1"
                     /translation="MNMSSRFGSRAITVIAILAALVLLSGCVPRLNVNPVLPDVVLVA
                     STPSTPETPDPNTPPPAGVADMHFVQPDDFFITEDALTDQGWIYARIGKMITPASEDT
                     KWQAQFLVTMDGSTVWTKYFKKTRIAKREDLVLGREVIFFDVTDQNGYYRYPESNQEG
                     RAGYWLNSRIVDVSELFKKVVMVAEGLKVNENAVRVIAE"
     gene            4492..>5174
                     /locus_tag="PLO35_05025"
     CDS             4492..>5174
                     /locus_tag="PLO35_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOH79062.1"
                     /translation="MLSTKDAIRDLQTREAKVRLMGGAKAVDKQVAGGKLTARQRLDL
                     LFDPGTFRELDMFVQHRCDNFGMEKIEIPSDGVITGHGMVDGRPVFAFSQDFTSRGGS
                     LGEMHAAKICKVMDMALKSGVPLVGIQDSGGARIQEGVDALKGYGDIFFRNSRASGVI
                     PQITAIMGPCAGGAVYSPAMTDFVFMVKNTSFMFITGPDVIRAVTGEETTQEELGGAM
                     SHNTHSGNA"
     assembly_gap    5175..5274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5275..5898
                     /locus_tag="PLO35_05030"
     CDS             <5275..5898
                     /locus_tag="PLO35_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOH79063.1"
                     /translation="IVGFARLNGRAIGIIANQPTVLAGCLDIDASDKATRFIRFCDAF
                     NIPLLTLVDVPGYLPGTHQEWNGIIRHGAKLLWCYSEATVPKLTVVTRKDYGGSYIAM
                     SSRHLGADMVFAWPMAEIAVMGAQGAANVIFRKEIDGAEDKAAKRAEMIAIYEQEFNN
                     PYVAASRGYVDAVIVPSETRARLIDALEILSTKSEALPPKKHGNIPA"
     gene            5910..6095
                     /locus_tag="PLO35_05035"
     CDS             5910..6095
                     /locus_tag="PLO35_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79064.1"
                     /translation="MMDKKELAAISAVMAIVNAEESLPFVERPEPQIQTAWQSWGARQ
                     TMQYRDITQRRIIKRTR"
     gene            6171..8162
                     /locus_tag="PLO35_05040"
     CDS             6171..8162
                     /locus_tag="PLO35_05040"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOH79065.1"
                     /translation="MDKHGILEMTAMRYEADRPQASHPIKIQDLSFRDGHQSLFATRG
                     RTEDLLHVAELMDQVGYYSMEVWGGATFDTMHRYLGEDPWQRIRTLKKYISKTPFSML
                     LRGQNLVGYQNYADDIVEAFVQRACDNGIDIFRVFDALNDFRNFQTAVKIILKNGKHF
                     QGTICYSLTEQRMGGEIYNTAYYVDKAKQLEEMGANSVCIKDMAGLVAPYDAYNLVKA
                     LKAAVKIPVHLHTHFTSGMGDLALFKAIEAGVDIIDTCLGPYAYRTSHPAVEPLVISL
                     LGTNRDTGFDIKLLNKISKEMEKDIPKYTQFADNTKYGIIDTDVIIHQTPGGMLSNLV
                     NQLRQMDNLDRLDDVFAEIPKVRKELGQIPLVTPTSQIVGIQAVNNALFDTKDGEYAR
                     ITEQVKDLCYGLYGKTTLPIDPEVQAKALKGYPRGETPITCRPGDVIEPGMPKVKEET
                     KGLAKDIDDELIVALYNLTGKKFLKIKYGLEPMPEDMKPTSLEEVKAREELVKKALAG
                     KLVEKSETPAPDKPEGLRQFDIFIGDEYFMVEVAEKGEAPRVVKRAPAPAPVAHTAAP
                     APAPAPAPKPAAVEAPKTAPVASGEGAPIPSPMPGMFVKYEVQVGDSVQVGQTVLVLE
                     AMKMYNNIPSPVKGTVVATPFSSGDTVAKGDALIVIKAD"
     gene            8173..8709
                     /gene="pyrR"
                     /locus_tag="PLO35_05045"
     CDS             8173..8709
                     /gene="pyrR"
                     /locus_tag="PLO35_05045"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOH79066.1"
                     /translation="MQIKSQIMDKAQMERSVHRMAHEIIEQNRGLAKICLVGIRSRGV
                     PLAQRLSEYLRLIEGQEIPVGMLDITLYRDDLSTIAHQPVIKGSQLDFDIEDAVIVLV
                     DDVLFTGRTVRAAIDALMDFGRPRQIQLAVLIDRGHRELPIKADYVGKNVPTSKEEII
                     KVEFEETDGSDRVCIVLA"
     gene            8675..9490
                     /gene="pyrF"
                     /locus_tag="PLO35_05050"
     CDS             8675..9490
                     /gene="pyrF"
                     /locus_tag="PLO35_05050"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOH79067.1"
                     /translation="MARTASASFWHKYRARWLEKGSLVCVGLDPDLDKLPDCVKHSPN
                     PIWEFNRSIIDATQEHACCYKPNLAFYLADGLRGLEALKKSIEYIPDAIPVILDCKVG
                     DIGSTMQGYVNAFFDELRVDAITINPLMGSDVLEPLLKREIGFAFALALTSNPSAADF
                     FTGAGMDKAVSQWMQSFPSQRLGAVVGATQTADLARMRALLPDRVFLIPGVGAQGGDL
                     KAVLQHAIASKANPDILINSSRGIIFASSKVDFAQAAATEAQKLYFEIQSLLR"
     gene            9533..11686
                     /locus_tag="PLO35_05055"
     CDS             9533..11686
                     /locus_tag="PLO35_05055"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOH79068.1"
                     /translation="MPQHLLRWYIRHYKHWSMLVIMAALLTLLMYALSDTRVMRNLEL
                     GSMDQRFVITPQPAKADTSIVMVAIDDASLKYVRDKLNMGWPWPRLNYGLITEFLADE
                     GARSTVFDISFEDPDLDRGDIDPALSDEDFALAIESSRNAVLAVTFTHYPTVGDSASI
                     NPAAFPPTYPYPDLITWQGALAPYPPFARVAHLGGINLASGDDSTIRRIPLIYRYKDG
                     YCPSLALAAYRIGRDSATEQRLILAQAQAPDGSMYLNWHGPAGPDGVFRYIPFSQLLE
                     CALAERNGFDPPRAKGWFKDRNVFIGAVASGLEDLKASPYTWGMPGMEVWATQLSNLL
                     GGNFVSFLPAWGDLLLIFLLSLLVMLLVTRLQGALASVSSFIVILVFTGVSILLFGSQ
                     RLALNYSSTLGALLISWLFTLSLSYTVEGRHKKELRRVFDRYLHPDLVKRIVDNPELV
                     SMGGEELQVTVMFSDIYNFTTFSEGKSPQDLVSYLNEYFRDLTGSVLDYHGLLDKYTG
                     DGLMAVFGAPIPREDHALLACKAALAHRRYSLAFAGLKELTPAQHFHLNTRLGINSGT
                     VVSGNIGSERRMEYTSIGDPVNLSSRLEGVNKVFQTHIIISDSTFQLVKDKMLCRELD
                     YLRVKGKHEATRIYELIEELDMIDLADYAWLDEFARALELYRQQDFPKAAAIFERLAA
                     PPLNDKPSLTMLERCQCLIKNPPEDWDGIFTLDEK"
     gene            11705..>12102
                     /locus_tag="PLO35_05060"
     CDS             11705..>12102
                     /locus_tag="PLO35_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79069.1"
                     /translation="MNQVIRPLLSAYVQWVTAYPIISAMIQFAILGTFGEIVSKWIIR
                     KSFKYPFSFALTLWKMIVWAILAVGIKYAFKGHTAMVAYLVEHQMLPPLDKFGTALAI
                     SVSLNTQFGLLLVIMHRVLDNLVEKHKNWAG"
     assembly_gap    12103..12202
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12203..>14289
                     /locus_tag="PLO35_05065"
     CDS             <12203..>14289
                     /locus_tag="PLO35_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOH79070.1"
                     /translation="GIIRISYRSPQDAILLKTLRERDHITQNAQFHIRKKLADKAYEL
                     RLTRLANSNFKQDLLLYHEDSLRVGTHYSAIVELLPIQRDPVLELFPSRYTARAYIRS
                     DLKSLGKEGHASLISQLRDWLNARLDRNAGDAAPIAKALLLSDQSAKKLYRDALTEGG
                     MVHLIVVSGLHVWFIYGILMLLLRSWLPRKPAELIFMVLISGFAALNFWAAPISRSMI
                     MILSVILARWMGRKVSSLQILSLSLFIITLAAPRQLFDLGLQLSFISVGVITLAVPRV
                     SLWSVKRHPLSGWRMASDRLLNYLLLSFMVSVAILPVTLYYFGRATLNGVIGNVVGVP
                     LVGMLLPLSLLVLVFSPQGLIGAGFNLAYRFGIWIFERWMLLCAALPFSLANAWIDLA
                     QVLGLLLLILPFMIWAKRKQTPLWRLFIPMWGLGLALLVLMPLVHPNQGGVWLFNCGT
                     ADCALIVSPTGHSILVDSGPSKSSWEAERDPSKALDTDSWASRKLVPWLKRRGIKHLD
                     ELIITHTHADHSGGVPALVTSLPVKRVIITDETRSSELWKLWRAQGWFDDSKLLLISD
                     TLSLDIARMKLSFLHPDRDWHTDSENDRSIVFRLDAKGKSFLFTGDIEAPSEAWLLAR
                     YPELLDVDYLKVPHHGSRTSNTAAFLKAVSPREAWISTAKRNRFDFPHPEALIHLRRH
                     SSQVRQTSDGTIH"
BASE COUNT         3225 a         4084 c         3735 g         3045 t
ORIGIN      
        1 aaatctctgt ccctccgtcc ctctgttttt aaaaaacctc tctgcttttc tctgtaacaa
       61 cctctgcttt tctctgtgtt gaagcaaacc ggcctaaacc agagttaggg gtacacgcaa
      121 gggcccttgc ccggcaggag ttcctcaaat cactaaacaa ctcaaaaata aggagaacaa
      181 gacatgaaag tcaccctcaa acaccgcctt gggatctacc agggcgaatg cgatgatctg
      241 gtctattact acaattccaa gctcaagacc atggtagccc ggcgctacac aaaaccgcgg
      301 gccagccagt ccaacgcccg tctgtcctca aacagcaagc acctgaaagc tttgaacccc
      361 tcggcagctt ttatcgcgga cctcaagctc tatcagctgc tccacaactc ggagctcaaa
      421 gccggagcta agctcatcaa caatcactac gccctctata cccgtctcat gtatgctcag
      481 gccagggctt tggacatccc agtcacggaa ctcagcagag ctctgataga gagcgctccc
      541 ttgccctgcc gctccgtgaa aaccgccgtg gagggtgggc ttatagaaga agtggagggt
      601 tatgacaggc tgacagggct gatctgagct gagctgggaa ctacattgcc acgggaagcg
      661 taaaggtccc tgttttcgtt ccgcgaattc cctcagcaga cgatgacgtc agccagtccc
      721 caagaccctg ccggcagcat ttatcaggtc ctgccaggaa atctcatcca ggcgtcattt
      781 gacgatagtg aatctgccag tgctgtgata ttttccggat tcaaccctga tcagatatat
      841 tccggatggg agtttgcggc cgctctcatc gcggcagtca tacaccgttg aatagatgcc
      901 accctgcccg gaagtcgaag cgggcactat gcccggtctg agagttctga gcctctgccc
      961 tttaaggttg tatatctgga ctatagcgtc ctctttcgcc agctttggaa gagtgaattc
     1021 gataaataca taagctgccc tggacgcggt cgcgcggaca ggattgggaa acagcgaaag
     1081 gacgatttga tcgggagctg aggggatatc cggctcggga ttggatacat aaggcacgga
     1141 gccatactca aagcagccca tgtccaccct gccgtcccag atccggagat tgccagccaa
     1201 atcagtgggg gggaggttca agccgctgac atccggagtc ccggcattga cgcagggcga
     1261 gattgaactt agctggtagg aggcaggcag agaggtatcc caggccggct ggccatgacc
     1321 ctcgaacagg ggatcttcgc caatcagcag attgctaatg ctgaccgcgg tggaatcctg
     1381 cacaccgacc ggaacggtaa acaggctgta ggaaactccg gggttgaagg tatccccgtc
     1441 attcacgttg ttgagcagca ggtcccgcaa atggttgtcc ggattataga aaatacagtt
     1501 gtagatatca ccgtatccgc gcagcaacat cacatagcgt ccccaggcat tgttgtaggc
     1561 aaaggtgctg ttgcggattt gcgcgcgctc aaactggttg aagatgtaga tgggcgcgcc
     1621 cgcatcggct gaagtggata tgttgttgat aaacagcata ttggaaaggt ccaggttgac
     1681 tccttccgtt acccggctct ggtacataaa catcgtgccg caattggcgt agcagtttga
     1741 gatcacggta ttgcgcacgg tggcgtccga ttcatagacc aacagaccgg aatggaaccc
     1801 gtcggacagg atcaccagat tgtcgatcag aatattgttc aggaagatat ttctggaact
     1861 caggaccgac acagcgaggt tgttatggtc gttggtcgtg gcgtttctgg ctgtgatgtt
     1921 ttcgcagatc acgccatcgc aatagccaat gcggatgcgg gtgaaatcgt caaaatagtc
     1981 ctctatattg ccgttccagc tcaggttccg cagttcgagg ttgtggacgc gagccgcaaa
     2041 aatcgggaaa gtgttggcgg tttcgatcac gtgagggata aaagtgatgt tctcgatcct
     2101 gatgttatgc tggttaaaaa atgacaaaca gcccctaccg ctattctcag ggttgaagat
     2161 cacaagctca ggatcgtcgc ccctcaaacg ggtgtgagat ttgagcgtca gcggaaaaaa
     2221 ctggccggaa ccggtaagat tgtattcgcc ggctgccaga tggatggttt tggctgccag
     2281 gctgtccgag gcgatgattt ttgccgctct ggcaatggtc ttgagcggat cttggggaga
     2341 cagaccggaa ttgccgtcat ctcctcccgg cgctacatac agatcgtggt tgaccaggtc
     2401 aaagtaagcg tgttgaactg aaatgctcag gtcctgggca ccgtcatact gaatgtagaa
     2461 ccagtccggt tcgctgagaa tcacactgaa ggtgtccaac accagattca ggggttgcgg
     2521 taaatccgcc agcaggatat ccatcccgtt gaaggaagtg ttgttgtaga tgctgtttgg
     2581 cagcaccgcg tcaaatacga tggaaaccag gcccgtgcct ccgatggcca ggcctccagg
     2641 tccgtctacg ccatagcact cgtatatttt ggtgttggac atatagaagt tgtcgccaac
     2701 aaagaaaact cctcccgcgc cctggccata acagtttctg attacgcagt tggtgatttg
     2761 gatgctggaa tcttcatata cttcgatgcc tccagctttc aggttccatc cgttcacatg
     2821 gggatgagac agcacttccg gcatgttgtt gacgagattg aacccgtcga tgatcacagt
     2881 ctcgtggttg tgcaccagca ggcaggaatt ggggaaactg gcccggataa tggtgccgtg
     2941 gatggtttcc tcatcctggg taaagatgta taaactgcgg atagtaaggt ttttgcccat
     3001 tgtgtccaga tgctcaaaat actctcccgg gctcacctga atgatatcac cgtgggtgct
     3061 gttgttgatc gcggcctgaa tgctggtgta gggttgcgtg ccatcgcttc ctacactcag
     3121 gacctcagcc agggccatgc ctggcagaaa aagcatggac aacgccagca acagcgtttt
     3181 gatttgcatc tgatacctcc tggcacctcg ccgcgggaat tttccccgca gccgaaaaca
     3241 catcggtgtc actataagtt cagcagcttc cgttgtcaag tgaaaggtta tggccggaga
     3301 gtaaaaccat ttggaggctg gtacttagaa cttcaaatca gtctcactgg gccaattgac
     3361 gctatcacat tatgggcaag caagatatga catccacacc tggtttgttg gactggggct
     3421 ttcccagatc aggctacccg gtttaatcac ccaaacttca aatctaccac catttactgc
     3481 cgcacttgga cacccctttg attctaccgg attcagtcct aaacagacaa taaaatgatc
     3541 tgaacgaagt tagcaaatct cgatcgttcg tctcgtctgg ctcagggtct tttgggcttg
     3601 gacatatgaa gtttttattg agggcaccta ggcggtctgc ccgtgggcaa aaaaagcttt
     3661 gacaagtatg gataccatgg caagtttgaa caagagacaa aaacatctgc aaaggagact
     3721 gttatgaaca tgtcctctcg ttttggatcc cgcgcgatca ctgtgatcgc tatcctggcc
     3781 gccctggttc tgctcagcgg ctgcgttccc cgcctgaatg tcaatccggt actgccggac
     3841 gtagtattgg ttgcttccac acccagcact cctgagaccc ccgatcccaa cactcctcct
     3901 cccgctggcg tagcggacat gcactttgtg cagcccgatg acttttttat caccgaagat
     3961 gccctcacgg accagggttg gatttatgcc cgcatcggca aaatgatcac tcccgcctct
     4021 gaagacacca aatggcaggc tcagttcctc gtcaccatgg acggcagcac cgtctggacc
     4081 aaatacttca aaaagacccg cattgccaaa cgcgaagacc tcgtgctggg cagggaagtg
     4141 atcttctttg acgttacgga ccagaatggt tattaccgct accccgaaag taatcaggaa
     4201 gggcgggctg gatactggct caacagccgc atcgtggatg tgagtgaact ctttaagaag
     4261 gtcgtgatgg tggccgaagg cctcaaggta aacgaaaacg ccgtcagggt catagctgag
     4321 taagcttttt taccctgcat caatctgaag ctgaagacgc aagcgtttgc gtcttcagct
     4381 ttttcttgac gaaataaggg gagtatccga ttctggttta tccggccccc aggctagtcc
     4441 acggggctca cacgcttatc ataatctact gatatacaag gggaaacact tatgctttct
     4501 accaaggacg cgatccggga cctgcaaacc cgcgaggcca aggtgcggct catgggcggc
     4561 gccaaagctg tggataaaca ggttgccgga ggtaaactta cggccaggca gcggctcgac
     4621 ctgctgtttg atcccggcac tttcagagag ctggatatgt ttgtgcagca tcgctgcgac
     4681 aattttggca tggagaagat cgagattcct tcggacggag tgatcaccgg ccacggcatg
     4741 gtcgatggcc gccccgtctt tgccttcagc caggatttca catcccgggg aggttctttg
     4801 ggcgaaatgc acgccgccaa gatctgcaag gtgatggata tggccctcaa gagcggtgtt
     4861 ccgctggtgg gcattcagga tagtggcggg gcccgcatcc aggaaggtgt ggatgccctt
     4921 aaaggctatg gagacatctt cttccgcaat tcccgcgcca gcggtgtgat ccctcagatc
     4981 accgccatca tgggaccctg tgccggtgga gcggtctatt ctcctgccat gacagacttt
     5041 gtcttcatgg tcaaaaacac ctctttcatg ttcattaccg gtccggacgt gatccgtgcc
     5101 gtcactggtg aagagaccac tcaggaagaa ctgggcggcg ccatgtctca caacacccac
     5161 agcggtaacg cgcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcgtg
     5281 ggatttgccc ggctcaacgg acgggccatc ggcatcattg ccaaccagcc caccgtgctg
     5341 gccggttgcc tcgatatcga cgcatcggac aaggccaccc gcttcatccg tttctgcgac
     5401 gccttcaaca tcccgctgct caccctggtg gacgttcccg gctaccttcc cggcacccat
     5461 caggaatgga atgggatcat ccggcatggc gccaagctct tgtggtgcta cagcgaggct
     5521 accgtgccca agctcaccgt ggtcacccgc aaagactatg gcggcagcta catcgctatg
     5581 agcagcaggc acttgggagc cgacatggta tttgcctggc ccatggctga aattgccgtg
     5641 atgggcgctc agggagcggc caacgtgatc ttccgcaaag agatagacgg agccgaggat
     5701 aaggcagcca agcgggccga gatgatcgcc atctatgaac aggagtttaa caacccctac
     5761 gtggccgctt cccgtggcta tgtggatgcc gtgatcgtgc cttccgagac ccgcgcccgt
     5821 ctcatcgacg cgcttgagat cctttccacc aagtctgaag ccctgccgcc caaaaagcac
     5881 ggcaacatcc ccgcttagga ccttctaaga tgatggacaa gaaagaactg gccgccatct
     5941 ccgcagtgat ggctattgtt aacgctgaag agtccctgcc ctttgtggag cgtcccgagc
     6001 cccagattca gacggcctgg caaagctggg gagcgcgcca gaccatgcag tatagagata
     6061 tcacccaacg cagaatcatc aagagaacga gatgagttca gaagcgcaaa cggggacgtt
     6121 tgcggttcca tatagacaaa agagttagat aaaaaaaagg agtatattag atggacaagc
     6181 acggcatcct cgaaatgacc gccatgcgct atgaggctga ccgcccccaa gcgtcccatc
     6241 ccatcaagat tcaggacctc agcttccgcg acggtcacca gtccctcttt gccacccggg
     6301 gcaggacaga agacctgctg catgtggccg agctgatgga ccaggtgggc tattattcca
     6361 tggaagtgtg gggtggggcc accttcgaca ccatgcaccg ttacctcggt gaagacccct
     6421 ggcagcgtat ccgcaccctc aaaaaataca tcagcaaaac ccccttctcc atgctgctgc
     6481 gtggccagaa cctggtggga tatcagaact acgcggacga tattgtggaa gccttcgtgc
     6541 agcgcgcctg cgacaacggc atcgatatct tccgcgtctt cgacgccctc aacgacttcc
     6601 ggaatttcca gaccgccgtc aagatcatcc tcaaaaacgg caaacacttc cagggtacca
     6661 tctgttacag cctcaccgaa cagcgcatgg gcggagaaat ctataacacc gcctactatg
     6721 tggacaaggc caagcaactg gaagagatgg gtgccaacag cgtctgcatc aaagacatgg
     6781 caggtctggt agccccctac gacgcctata atctggtcaa agccctcaaa gccgcggtga
     6841 aaatcccggt gcatctgcac actcacttca ccagcggtat gggagatctg gccctcttta
     6901 aagccatcga agctggagtg gacatcatcg acacctgcct gggaccctat gcctaccgca
     6961 cctcccatcc tgctgtggaa ccgctggtta tcagcctgct gggcaccaac cgcgacaccg
     7021 gtttcgacat caagctgctc aataaaatct ccaaagagat ggagaaggac atccccaaat
     7081 atacccagtt cgcggacaac accaaatacg gcatcatcga taccgacgtc atcatccacc
     7141 agacccctgg cggcatgctt tccaacctcg tgaaccagct gcgtcagatg gacaatctgg
     7201 accgcctcga cgacgtgttt gccgagattc ccaaggtgcg taaagagctg ggacagatac
     7261 cattggtgac tcccaccagc cagatcgtgg gtatccaggc cgtcaacaac gccctcttcg
     7321 acacaaaaga tggtgaatat gcccgcatca cagagcaggt caaagatctc tgttatggcc
     7381 tctacggcaa gacgaccctg cccatcgatc ccgaagtgca agccaaagcg ctgaaaggat
     7441 atcccagagg cgaaactccc atcacctgcc gtcccggaga cgtgatcgaa cccggcatgc
     7501 ccaaggtgaa ggaagagacc aaaggcctgg ccaaagacat cgacgacgag ctgatcgtgg
     7561 cgctctacaa cctcaccggc aagaaattcc tcaagatcaa atatggcctg gagcccatgc
     7621 cggaagacat gaagcccacc agcctggaag aagtgaaagc ccgtgaggaa ctggtcaaaa
     7681 aagccctggc cggcaagctg gtggaaaaga gtgagactcc cgctcctgac aagccggaag
     7741 gcctgcgcca gttcgatatc ttcatcggag acgaatactt tatggtggaa gtggcggaaa
     7801 aaggcgaggc ccccagagtg gtgaaacgcg ctcccgctcc ggctccggtt gctcacaccg
     7861 cggccccagc tccagcccca gctcctgctc ccaaaccggc tgccgttgag gctcccaaaa
     7921 ccgctccggt ggcttcagga gaaggtgctc ccatcccgtc ccccatgccc ggcatgtttg
     7981 tcaaatacga ggttcaggtg ggcgacagcg ttcaggtggg ccagaccgtc ctggtgctgg
     8041 aagcgatgaa aatgtacaac aacatcccct ccccggtcaa aggcactgtg gtggccacac
     8101 ctttctcctc aggagacacg gtggccaaag gcgatgccct gatcgtgatc aaagcggatt
     8161 aggagattta agatgcagat aaaatcccag atcatggaca aagcccagat ggaacgcagc
     8221 gtccatcgta tggcgcatga gatcatcgag caaaaccgcg gccttgccaa gatttgtctg
     8281 gtgggcatcc gctcccgggg agttccattg gcccagcgcc tttcggaata cctgcgcctc
     8341 atcgaaggcc aggagatccc ggtgggtatg ctggatataa ccctttacag ggatgacctt
     8401 tccaccattg cccatcagcc ggtgatcaag ggctcccagc tcgatttcga tatcgaggat
     8461 gccgtcatag tgctggtgga cgacgttctg ttcactgggc gcaccgttcg agccgccatc
     8521 gacgcgctga tggattttgg caggccacgc cagatccaac tggccgtgct gatcgaccgc
     8581 ggtcatcgtg aactgcccat caaggctgat tacgtgggca aaaacgtgcc cacctccaag
     8641 gaagaaatca tcaaagtgga atttgaggag acggatggct cggaccgcgt ctgcatcgtt
     8701 ctggcataaa taccgtgccc gctggctgga aaagggatca ctggtctgcg taggcttgga
     8761 tcccgatctg gacaagctgc cggactgcgt caaacactca cccaacccca tctgggaatt
     8821 caaccgttcc atcatcgatg ccacccagga gcatgcctgc tgctacaagc ccaatctggc
     8881 cttctacctt gccgacgggc tcagaggcct ggaggccctg aagaagagca tcgagtatat
     8941 cccggatgcc atccccgtga tcctggattg caaggtggga gacatcggca gcaccatgca
     9001 gggctatgtg aacgccttct ttgacgagct cagagtggat gccatcacga tcaaccccct
     9061 catgggctcg gacgtattgg agcctctcct gaaaagagaa atcggctttg cctttgccct
     9121 ggccctcact tccaaccctt ccgccgcgga tttctttacc ggagcgggta tggacaaagc
     9181 tgtgagccag tggatgcaaa gcttcccctc ccaaaggctt ggggccgtag tgggcgccac
     9241 tcagacagcc gacctggcca gaatgagagc gctgctgcca gaccgcgtct ttctcatccc
     9301 cggagtgggc gctcagggag gagacctcaa ggcggtgctc caacacgcca tcgcctccaa
     9361 agcaaacccg gacatcctga tcaattcctc ccggggcatt atctttgcct cctccaaagt
     9421 ggattttgct caggctgctg ccacggaagc ccaaaagcta tatttcgaga tacaaagtct
     9481 gctgcgatag gttacgcgta acgcgtgacg cgatagccag ggaggtttca ctatgccaca
     9541 gcatctattg cgctggtata taagacacta caagcactgg tccatgctgg tgatcatggc
     9601 ggccctgctc acgctgctga tgtacgctct gagcgacacc cgcgtgatgc gaaatctgga
     9661 actgggcagc atggaccagc ggtttgtgat caccccccaa cccgccaagg ccgacaccag
     9721 catcgtgatg gtggctatcg acgatgcctc cctaaaatac gtgcgcgaca agctcaatat
     9781 gggatggccc tggccgcgcc tgaattatgg tctgatcacc gaattcctgg ctgatgaggg
     9841 tgcccgctcc accgtcttcg atatcagctt tgaagatccc gatttggacc gcggagatat
     9901 tgaccctgcc ctttcggacg aggatttcgc gcttgccatt gagtccagcc gcaatgctgt
     9961 cctggccgtc accttcaccc attatcccac tgtcggagac agcgccagca tcaatcccgc
    10021 tgccttccca ccgacatatc cctatccgga cctgattacc tggcagggag ctctggcacc
    10081 ctatcctcct tttgcccggg tcgctcatct gggaggcatc aatctggcca gcggagacga
    10141 ctccaccatc cgtcgcatcc ccttgatcta tagatacaaa gacggctact gtcccagcct
    10201 ggcattggct gcttacagaa ttggaagaga cagtgccacg gagcagcggt tgatcctggc
    10261 tcaagcccaa gctccggatg gcagcatgta tctgaactgg catggtcccg ccggtcctga
    10321 tggtgtgttt cgttacatcc ccttcagcca gcttctggag tgcgctttgg cggaaaggaa
    10381 tggctttgat cccccccgcg cgaagggatg gttcaaggac cgcaacgtct ttatcggagc
    10441 ggtggccagc ggtttggaag atctcaaagc cagtccttac acctggggca tgccaggcat
    10501 ggaagtgtgg gccactcaac tcagcaatct gttgggaggc aactttgtaa gctttctgcc
    10561 cgcctgggga gacctgctcc tgatcttcct gctcagcttg ttggtgatgc tgctggtcac
    10621 gcgtctgcaa ggggccctgg cctcggtttc ttcctttatc gtgattctgg tcttcacggg
    10681 cgtcagcatc ctgctctttg gcagccagag actggcgctc aactacagct ccaccctggg
    10741 ggcgctgctg atctcctggc ttttcaccct cagcctcagc tacaccgtgg aaggccgcca
    10801 caaaaaagag ctgcgccggg tcttcgaccg ctatctgcat cccgaccttg tgaaacgcat
    10861 cgtcgataac cccgagctgg tttccatggg tggtgaagaa ctgcaggtaa ccgtcatgtt
    10921 cagcgatata tacaacttca ccactttctc cgagggcaag tccccccagg acctggtgag
    10981 ctatctgaat gagtactttc gggatctcac cggcagcgtt ctggactacc acggcctcct
    11041 ggacaaatat accggcgatg gactgatggc cgtgttcgga gctcccattc ccagagagga
    11101 tcacgccttg ctggcatgca aggcagctct ggcgcaccgg agatacagcc tcgctttcgc
    11161 gggcttgaaa gagcttacac ccgcccagca ctttcatctc aacactcgcc tggggatcaa
    11221 ttccggcaca gtggtgagcg gaaacatcgg cagcgagcga cgcatggaat acacctcgat
    11281 cggcgatccc gtcaatctct catcgcgcct ggaaggtgtg aacaaagtgt tccagaccca
    11341 tatcattatc agcgacagca ccttccagct tgtcaaggat aagatgctct gccgggaact
    11401 cgactatctg agggtcaagg gcaagcatga agccacccgc atctatgagc tgatcgagga
    11461 actggacatg atagacttgg cggattatgc ttggctggat gaattcgcca gagctctgga
    11521 gctttaccga caacaagatt ttccgaaggc cgccgccatc tttgagcgcc ttgccgctcc
    11581 gcctctgaac gacaagccca gcctcaccat gctggaacgc tgccaatgcc tgatcaaaaa
    11641 tcccccggaa gactgggatg ggatcttcac cctggatgag aaataaacca ataaaggaca
    11701 catcatgaat caagtcatca gacccctcct gagtgcttac gtgcaatggg tcacagccta
    11761 tccaatcatc tccgccatga tccagtttgc catcctgggc acctttggag agatcgtctc
    11821 caaatggatc atccgcaaga gcttcaagta tcccttcagc tttgccctca cgctctggaa
    11881 gatgatcgtg tgggccatcc tggccgtggg tatcaaatac gccttcaaag gccacaccgc
    11941 catggtggca tacctcgtgg aacatcagat gctgccgcct ctggacaagt ttggcaccgc
    12001 cctggcaatc tccgtctcgc taaacaccca gtttggcctg ctgctggtga tcatgcaccg
    12061 ggtgctggac aacctggtgg aaaagcacaa aaactgggct ggnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nngggatcat caggatcagc taccgttccc ctcaggatgc
    12241 cattctcctc aagacgctga gggaacggga ccacatcacc caaaatgctc agtttcatat
    12301 ccgtaaaaag ctggcagaca aggcgtatga gctgcgcctg accaggctgg cgaacagcaa
    12361 cttcaaacag gacctgctgc tctaccacga ggactctctg agggtgggca cccactacag
    12421 cgctattgtc gagctgctgc ccatccagcg cgaccccgtt ctggagctct ttccatcccg
    12481 ttatactgcc agagcttaca tccgttcaga cctcaaatct ctgggtaaag aaggccatgc
    12541 gtccctgatc agccaactcc gcgattggct caatgcgaga ttggatagaa acgccggaga
    12601 cgctgctccc atcgccaaag ccctgctgct ctcggaccag agcgccaaaa agctctaccg
    12661 ggacgcgctt acggaaggag gaatggtcca tctgatcgtg gtcagcggtc tgcacgtgtg
    12721 gttcatctac gggatcctga tgttgctgct cagaagctgg ctgccacgca agccggccga
    12781 gctgatcttt atggtgctga tcagcgggtt cgcggcgcta aacttttggg ccgcgcccat
    12841 cagccgttcc atgatcatga tcctgagcgt gatcctggcc agatggatgg gcaggaaggt
    12901 ctccagcctc caaatcctca gcctcagcct ctttatcatc actctggccg ctccccgtca
    12961 gctctttgac ctcggtctgc agctctcgtt cattagcgtg ggagtgatca ccctggcagt
    13021 gccgcgcgtc agtctgtggt ccgtcaagcg tcatcccctt tcaggctgga gaatggcctc
    13081 agaccggctg ctcaactatc tgctgctcag cttcatggtc tcggttgcca tcctgcccgt
    13141 caccctctac tattttggca gggctaccct caacggtgta attggcaacg tcgtgggagt
    13201 accgctggtg ggaatgctct tgccgctttc cttgctggtg ctggtctttt caccccaggg
    13261 cttgatcgga gcgggcttca acctggccta tcgctttgga atatggatat ttgagcgctg
    13321 gatgctgctc tgcgcggcct tgcccttcag tctggccaac gcctggatcg atctggccca
    13381 ggtgctgggc ctgctgctgc tcatcctgcc attcatgatc tgggccaagc gtaaacaaac
    13441 tcctctctgg cggttgttta tccccatgtg gggtctggga ctggctttgc tggtcctcat
    13501 gccgctggtc catcccaatc agggaggcgt ctggctattc aattgcggga ctgccgattg
    13561 cgctctaatc gtgagtccaa ccggtcacag cattctggtg gacagcggcc ccagcaaatc
    13621 cagttgggaa gccgaacgcg atccctccaa agctctggat accgatagct gggcgtcccg
    13681 caaactggtc ccctggctca aacgacgcgg catcaagcat ctggacgagc tgatcatcac
    13741 ccacacccat gccgatcaca gtggaggcgt gccggctctg gtgacctctc taccggtgaa
    13801 aagggtcatc atcacggacg agacccgctc ctcagagctt tggaagctct ggcgcgcgca
    13861 aggctggttt gatgattcca aactcctgtt gatcagtgac accctcagcc ttgatatagc
    13921 caggatgaag ctcagtttcc tgcatcccga tcgcgactgg cacacagaca gcgaaaacga
    13981 ccgcagcatc gtcttccgcc tcgatgccaa aggcaagagc ttcttattta caggagatat
    14041 cgaagccccc tcggaagcct ggctcctggc cagatatcct gaactcctgg acgtggacta
    14101 tctcaaagtg ccccaccacg gcagccgtac ttccaacacc gctgcctttc taaaagccgt
    14161 ttcccctcgc gaagcatgga tcagcaccgc caaacgcaac cgcttcgatt ttccacatcc
    14221 tgaagcgctg atccacctga ggcggcacag ctctcaggtt cgccagacat cggacgggac
    14281 tatccactt
//
LOCUS       DAOSFN010000056        14170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3560_length_14170_cov_9.904640, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000056 DAOSFN010000000
VERSION     DAOSFN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14170
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3560_length_14170_cov_9.904640"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..725
                     /locus_tag="PLO35_05070"
     CDS             279..725
                     /locus_tag="PLO35_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOH79071.1"
                     /translation="MQDHEKLFTQYLATRGLRLTKPRRQILNTVFAMHEHFNAEQLYD
                     RMRILSSDVSRATIYRTLPLMMDAGLIQHSLRFAAKDVYEHIFGHPKHLHWICRKCGN
                     VTETSLSAILPKLEAAAREQHFKLEDYNLNLQGLCWKCRQSENESQ"
     gene            729..2852
                     /gene="feoB"
                     /locus_tag="PLO35_05075"
     CDS             729..2852
                     /gene="feoB"
                     /locus_tag="PLO35_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOH79072.1"
                     /translation="MSTNNPVIALAGNPNVGKTTLFNALTGSHQVVGNWPGVTVEHKQ
                     GYYLYQGKRYEVIDLPGIYSLSGGTPDELVARDFILHNKPDLVINIVDSSNLERNLYL
                     SVQLMELGAPMLMLLSMCDITVRNGIRVDDQHLSSHVGIPVLPATLNHGFDSSALLER
                     IACTLKDAPKPALIRYDEVVETALDRIVPQLRSMDSETGPIQLRWQALKLLEQDPALE
                     LKLNDELRLLVEKEIKAIEKHRGQASPNLIADDRYGFIRGLAKDVITRASENKLTYSD
                     RVDKIALSGVLGLPLFFFVMYLVFLLAVRASGPIIDLIDGGLSWLLVEQWGALLRSLH
                     LPEGLVFMLSDALGGGIVTIGTFVPPIFFIFASLSILEDSGYMARAAFIADKFMRRIG
                     LPGKAFIPLLVGFGCTVPAIMATRTLESKRDRVFASLLSPFMSCGAKLPVYTYLAMVF
                     FPRRADLVIFGLYFFGVLMAMIFGLMLKKTLFKTQPGNFVMELPPYHFPTFNGIMLHT
                     WHRLKDFVLRAGKTILTVILVMNLLQVITLPSASSPEGKISVLELAGKGLTPLLAPMG
                     IRSENWQAGVALVSGLFAKEAIVGTLQGLYQGSGTEEAAETALLSGFGSTAAVLAYLV
                     FVLLYSPCAAALAMLFKEHGWRWMAFSFAYLTLLAWIMATLVYQLLVFSAASFIWIGI
                     ALLMGLGFYFSMQLLGRSKHHAFQS"
     gene            2836..3138
                     /locus_tag="PLO35_05080"
     CDS             2836..3138
                     /locus_tag="PLO35_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOH79073.1"
                     /translation="MLSNRNRARLRQFGRRFFYNPRPCNCKRGDCVPLTELHENDSAI
                     ITRNNDLRIIERGMYHGMRISVFRNEPDEPNLIIAVGDSRYVLDRRIAALIMVRVD"
     gene            complement(3342..4454)
                     /locus_tag="PLO35_05085"
     CDS             complement(3342..4454)
                     /locus_tag="PLO35_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOH79074.1"
                     /translation="MESKLIYAVIIMLTMLLPCACVKAEPQKPASQEGTSGEQSTVWF
                     TNEISPQAVSRIYAKLSGELNGKIAIKLHFGEEGNQNFLPASLIKDLCLNLDATLVET
                     NVLYGGPRSKTASHIALARKHGFDFAPIDILDDEANLAFEARAKHFSKVYTGSHFTDY
                     DGYLIFSHVKGHGNAGFGGAIKNIAMGLASREGKRFQHQGNYPVYDSEKCISCGICVS
                     SCPETAITIDPVNIDHTACIACGKCISVCPVKVFSQSPGGRSAEAFQERLVEYAKVLS
                     SQRKMVYINVLANISSACDCMSHSPKPFVKDIGILASTDIVAIEAASYDLVNKAHNCE
                     DAFLKESRVSGRHQIDYAHAIGMGHKDYKLVDIDAK"
     gene            complement(4481..6772)
                     /locus_tag="PLO35_05090"
     CDS             complement(4481..6772)
                     /locus_tag="PLO35_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOH79075.1"
                     /translation="MRLAALIILLTAILALGAQNLPTCYYTYAQISQMLSDHEAQHPD
                     IAKKVQIGVSQQEGLPIYAMRISDNVNNDEQEPAILLVGQVHAEEVLGVQITMSNIAE
                     ILANRYQLPYNQWINQLDIWLIPSLTPEGHNVVTANLDTSYRKNKRDNNNNGVFDYSN
                     LVGYDIDGVDINRNFSFNWVQGDTLMQPGGLEVWDYYRGPAPMSESEVQAVYNLAEQY
                     RFVYSIVWHSSRTGNLSEKCYYPFNWKEVRPSPDVNQAAAISAGIAAQITKEAGGATY
                     ESLPNLSRKGAFHDWMYQQYGTIQILIECGTRNLQPDSLLMVNTVQRCSNGVRWLLNR
                     ALPYSSDVPTSAMLTGIVKDSVTNQPLEAEVIIQQHHADWLRPRLSYATNGRYYRSLQ
                     TGNYTVIGKKQGYFDTVLPSVTINNTNWTPRDLILTPRQPATLSGHVQSGGIDIPALI
                     VLGNTWVDSLVVNGAYVYQAFEGEYPITIHADGYYPYQGSVTLTPGANHRSFDLSPAT
                     VNFSEDWEDGTANWQIEGPWVVQNEQSVSGYAITDSWGGRGFYAQNCDVWIQTVNPIS
                     IPASGDPLLSFDSHLYTEPVHDPVKVQISADGSTWTTLWESSGRKDWWQRQYVDLSDY
                     PGSYYLRFRLTDESIHIELTDPGWTIDNIRVITGSAVADGQLLNPALPSLALYPNYPN
                     PFNPSTTIAFANSQSTDMKLEIFNLKGQRVRLLVDEQLPAGNHQAVWNGQDDAGRSVA
                     SGMYMYRLTGQGYSKTYKMMLLK"
     gene            complement(7026..7631)
                     /locus_tag="PLO35_05095"
     CDS             complement(7026..7631)
                     /locus_tag="PLO35_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOH79076.1"
                     /translation="MHKLAKMNTPVFWVEGHPGKLDRESWTLEVTGLCDQPRTFSWQE
                     LMKLPKSTADARLTSVTRWSVRGIWGGVALSDLLREVGMRDSCKYIRFWSHGLVYDTS
                     IPLDIALKEKTIVAWEFDGELLTEDYGGPIRAFTPYLWGYKSAKSIVRIELMDHYVPG
                     FWELRGYTDSAEIEAGPMRDINDGGKVKQIPGGEVLDFISR"
     gene            complement(7739..9259)
                     /locus_tag="PLO35_05100"
     CDS             complement(7739..9259)
                     /locus_tag="PLO35_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOH79077.1"
                     /translation="MIPILTVEQMRAVDGKAITELGIPSLVLMENAGRGCVTKILDHF
                     KLSKDGYVLIVCGPGNNGGDGAVIARWFNILGYNTLIILTDSEKASPDQKHNLELCEK
                     LKIKIMSMPEKESELKFYLDNAALIVDAIYGTGFQAMMPDHVIQLVERINESRTPVCS
                     VDIPSGIHGDNGLTGICVEAELTLAMETYKPGHFFGRAKLYCGKLERVPIGINETMYE
                     DISHTWLANYEDLPSDGRFATAYKSMYGRVVIIGGREGYTGASLLATKAALRTGAGYV
                     YVYHRKELASLYASRLTEALYVSVPEDDEGLPEAQQLTELWKDASVILIGPGLGRDPW
                     ALRILNIVLEQHATPLVIDADALNLISENRDMLPLLDRYELVITPHWGEFCRLADINM
                     DDLYNDPIGKLRSFLPESPVTILLKSHFSLIRHEEDVFITNSGNDGLATGGSGDVLAG
                     IVASRIAQNEHMALSAIAGSLILGRAAEQLATKRGTASILPSDVVEEILVHPRTES"
     gene            complement(9256..10815)
                     /locus_tag="PLO35_05105"
     CDS             complement(9256..10815)
                     /locus_tag="PLO35_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79078.1"
                     /translation="MKPVFRNSTQSGTSRKSTNRFQTLRLYLIIGTVVIFVAFAIYTE
                     ILIRNARREQEYVPRIFAQYIAYTDSYLRQAEQYEDLLSEVRTHYFQFASQRNFQEAL
                     WDYISLEFMQKNPIPVIITDEAYTPLFWNKVGISQDSLYSELSYRSQALLQEQMANMN
                     QTPLTDEDMVTGYAFYAKPVSLQQFISKVDYSIIVTDRQKVPLFWRNVDIPEVARYSE
                     IGENDRQHLRDRIDAMAEIPIASEADSLGYIYFSLPRSLSQIRNFVVLELLIAVLLIA
                     FSSYGLFLLRRTEKDTLWIGLAKETAHQFGTPITSLMGWIDYLKSTAPGSEPQMPTGQ
                     IIDFMTTDLEHLRNIASRFGKVGSQVKLQPAPMHDILSETVEYFRHRMPHLGSRIDIH
                     LISKIEGVNVMLDAELFKWTLENLIKNCVDAMTSRGGNIILTATRKDKWVYLQIRDEG
                     KGIPRSQWKKIFEPGVTTKKRGWGLGLSLAKRIIEEYHQGHIKVLESTVNEGTTFEIK
                     LHTEDHKKEHL"
     gene            complement(10760..11527)
                     /gene="mazG"
                     /locus_tag="PLO35_05110"
     CDS             complement(10760..11527)
                     /gene="mazG"
                     /locus_tag="PLO35_05110"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOH79079.1"
                     /translation="MQEFDDLVQIIARLRDPVSGCPWDIKQTPKSLTANFIEELYEAV
                     EAIEDEDDVSLSEELGDLMLHIIFQAQIAVEEDRFDIREVLSKINDKLVRRHPHVFAD
                     HHVEGAEAVKMNWERIKKREKKERKSVLEGIPRSLPGLIQAQRTQEKAASVGFDWPDL
                     QPVLAKLEEEQEEFHAALASGQLENIREELGDLLFTIVNLARKLHIDAESALKATTRK
                     FAKRFNYIEDHYESSGKDIHEASLQELDAVWDLAKKH"
     gene            complement(11514..12809)
                     /locus_tag="PLO35_05115"
     CDS             complement(11514..12809)
                     /locus_tag="PLO35_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79080.1"
                     /translation="MKQHKSLSSLILMAFGLYALVEFYPFHPALFYAPVIMVALFCLA
                     WVLIYPQTRLIDAVNARPLLFLALWFIYNLLSYLWAVDSKEVLLYALLIARYSVMFMA
                     FDRLLRKPGLLKLAPWFLSGILLLYVLTALWELTSFHHLPSSRLYGDWHYVPTGPFYN
                     ENNLAAYMMLFTPFLLFLPKLSGKWWLGILAALITVIVLVLMTIQGARIGILVMAVFL
                     AWFFIWRVGLRQKLILGLAVLMLGGALWLRFHKEIKLVLLVLKHQTSSIGSESGSVYM
                     SSIKIRQQLIGETVLMAASTGFMGVGGGNYEPYMSADALLRTGWITNPHNFLMELFGN
                     WGIIILAAFLYLYGHWIWRLFKLARGSDGKERAWNLMWLSSLILFIFASILPSSIRWN
                     YFVWIYLAAVNVAIHIPFNKIYPVTLKAETPEEEALAGI"
     gene            complement(12806..14005)
                     /locus_tag="PLO35_05120"
     CDS             complement(12806..14005)
                     /locus_tag="PLO35_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOH79081.1"
                     /translation="MRKELGLFDLLYVIAKHKWLVIVFTAVFAVGSIIYALVTPKHWV
                     SKTVIVPVADNASLSGLDSGLLGMIGSGLLSGSQTDPAVEFVSIMRSRSFRERVINEF
                     DLLKYFKLTDNPRDEAMELAVFKVSSKLAKINADEESKMITVRIETKDKLFSKKIAEF
                     YLAELQDYLRNNRGSKSKLQREFLESQVAVTKLTIDSLATAVKQFQQKNRAIGLDEQT
                     ASLITLYSESISDFYKAEMEYNVALKQYGEGSPVLEELASRRDYLKTKVKELEGSGSK
                     LMPEYVIQIDRIPDLSLQFAQLKLNLEIQKKVFEYLYPQYEIARLDEARDMPSFDILD
                     PPSMAGLRSKPKRALLVSIVTFMAFLLACFVAIVKENVINTHKAEIDRVLSALKSRPA
                     SDSDSQM"
BASE COUNT         3071 a         3765 c         3951 g         3383 t
ORIGIN      
        1 cggggcagcg tgacacatga tcaggccaga gctttctgcc cggcagttca ttacgctggg
       61 atttgagcca gctagataag cagcctcacg gacttcagaa tcccaatcca gttcctcatt
      121 cccgcctcaa gatattgatg tatatacttt ttttactagt gagttaggac attagtttag
      181 tatgatgatt cacccccggt atggcctggt ctgcagatat ctattgacag aatcagcccc
      241 gaaataatat gcgactgaat ctcattaagg ataaagccat gcaagatcac gaaaagctct
      301 ttacccagta tctggctacc cgcgggctga ggctgacaaa gccccgcagg cagattctga
      361 acacggtgtt tgccatgcac gagcatttca acgccgagca gctctatgac cggatgagga
      421 ttctgagctc agacgtctcc agggccacga tctaccgcac cctgcccttg atgatggatg
      481 ccgggctgat ccagcattcg ctacgctttg ccgccaaaga cgtatatgag catatcttcg
      541 gtcaccccaa acacttgcac tggatctgcc gcaaatgcgg aaacgtgacc gagacgtctt
      601 tgagcgctat tcttcccaag ttggaagcag cggccagaga gcagcacttc aagctggaag
      661 actataacct caaccttcag gggctctgct ggaagtgccg gcaatctgag aatgagagtc
      721 aataaagcat gagcacaaac aaccccgtca tcgccctggc cggcaacccc aacgtgggca
      781 aaaccaccct gttcaacgcc ctcacgggaa gccatcaggt ggtgggaaac tggcccggcg
      841 tgaccgtaga gcacaagcag ggctactacc tctatcaggg caaacgttat gaagtgatcg
      901 acctcccggg catctattcc ctgtcggggg ggactcccga tgagctggtg gcacgggact
      961 ttatcctgca taataagcca gacctcgtga tcaacatcgt ggatagctcc aatctggagc
     1021 gcaacctcta tctcagcgtc caactgatgg aactaggcgc tcccatgctg atgctgcttt
     1081 cgatgtgtga catcacggtc cgcaacggca tcagggtgga tgaccagcac ctcagttcgc
     1141 atgtgggaat tccggtttta cccgccaccc tgaaccacgg cttcgacagc agcgccctgc
     1201 ttgaacgtat tgcctgcact ctgaaagatg cccccaaacc cgctcttatc cgctatgacg
     1261 aggtagtgga aaccgctctg gacaggatag tgccacagct ccggtccatg gatagcgaga
     1321 cgggcccgat tcagctccgc tggcaagctc tcaagctgct ggaacaggac cccgccctcg
     1381 agctgaaact gaacgacgag ttgcgcctcc tggtggaaaa agagatcaag gccatcgaaa
     1441 aacaccgcgg ccaggcatcg ccaaacctca tcgccgatga ccgttacggc ttcatcaggg
     1501 gtctggccaa agacgtcatc acccgcgcca gcgaaaacaa gcttacctac tccgaccggg
     1561 tggacaagat cgccctcagc ggggtgctgg gactgccact tttctttttt gtgatgtatc
     1621 tggttttcct gctggcagtc agggccagcg ggccgattat cgacctcata gacggtggcc
     1681 tttcctggct ccttgtggag cagtggggag ccctgctgag gtcactgcat ttgcctgaag
     1741 ggcttgtctt tatgctctcg gatgctctgg ggggaggcat tgtgacgatc gggacttttg
     1801 tgccgcccat cttcttcatc tttgcctcgc tctccatttt ggaggacagt ggttacatgg
     1861 caagggcggc ctttatcgcg gataagttta tgcgccgcat cgggttgccg ggtaaagcct
     1921 tcatccctct gttggtagga tttggctgca ccgttccggc catcatggcc acccgcactc
     1981 tggaaagcaa gcgggaccgc gtgtttgcct cgctgctttc tcccttcatg agctgcgggg
     2041 ccaagctgcc cgtttatacc tatctggcca tggttttctt tccccgcagg gcggacctgg
     2101 tgattttcgg cctctatttc tttggggtgc tgatggcaat gatctttggc ctgatgctca
     2161 aaaagaccct cttcaaaacc caacccggca attttgtgat ggagctgccg ccttatcact
     2221 tccccacctt caatggcatc atgctgcaca cctggcaccg gctcaaagac tttgtgctga
     2281 gggccggcaa gaccattttg accgtgatcc tggtgatgaa tctgctgcag gtgatcactc
     2341 tgcccagcgc ttccagcccg gaaggcaaaa tcagcgtgct ggaactggcc ggaaaaggtc
     2401 tcacgccgtt gctggcaccc atgggcatca ggagtgaaaa ctggcaggcg ggagtggcgc
     2461 tggtttcagg tctgtttgcc aaggaagcca ttgtgggcac cctgcaggga ctctaccagg
     2521 gctccgggac cgaagaggct gccgaaaccg ctctgctgtc gggctttggc tccaccgcgg
     2581 ccgtgctggc ctatctggtg tttgtattgc tctattcacc ctgcgcggcg gctctggcga
     2641 tgctctttaa agaacacggc tggcgctgga tggccttttc ttttgcctat ctcaccttgc
     2701 tggcctggat tatggccacg ctcgtatatc aattgcttgt cttcagcgcc gcctctttta
     2761 tctggatcgg gatcgcgctg ctcatgggcc tgggcttcta tttttccatg caacttttag
     2821 ggagatcaaa acatcatgct ttccaatcgt aaccgcgcca gattgcgcca gttcggtcgc
     2881 cgtttctttt acaaccccag gccctgcaat tgcaagcggg gagattgtgt accgctcacc
     2941 gagctgcatg aaaacgacag tgccatcatc acccgcaaca acgatctacg gatcatcgag
     3001 cggggaatgt atcacggcat gcggatcagc gtcttcagaa acgagcctga tgagcccaac
     3061 ctcatcatcg ccgttgggga ttcgcggtat gtgctggatc gcaggatcgc cgctctcatc
     3121 atggtgcggg tggattgagt gtgagtggcg gggttttagg gttttagggt tttaggggtt
     3181 taggggttag caggctacct gccatctcaa ccatataacc ttccctataa gccacccatg
     3241 cttatcccgt gcttatcgca cgcctatcgc gtgcttaagc atgggtggag tatgggagac
     3301 gggaaggagg cgttcaggat actggcaagc aggccagcgg gttatttggc gtctatatcc
     3361 accagtttat agtctttgtg gcccattccg atagcgtggg catagtcgat ctggtggcgt
     3421 ccgctgacgc ggctttcctt gaggaaggcg tcttcacagt tgtgggcttt gttgaccagg
     3481 tcataggaag cagcttcgat ggccacgata tccgtggagg ccagaatgcc gatatccttc
     3541 acgaaaggtt tgggagagtg agacatacag tcacaagcgc tgctgatatt ggcgagaacg
     3601 ttgatataga ccatcttgcg ctggctggac agcactttgg catattccac cagtcgctcc
     3661 tgaaaggcct cggcactgcg acctcccgga gattgcgaaa acaccttcac cgggcacaca
     3721 gaaatgcatt ttccgcaggc tatgcaggct gtgtgatcga tgttgacagg atcaatggtg
     3781 atggcagttt ccgggcatga acttacgcag attccgcagg agatgcattt ctcgctgtca
     3841 tacactggat agttgccttg gtgctgaaag cgttttccct cccggctggc caggcccatg
     3901 gcaatgtttt tgatggcccc gccaaacccg gcattgccat gacctttcac gtgagaaaag
     3961 atcaaatagc cgtcgtaatc agtgaaatgg ctaccagtgt aaactttgct gaaatgctta
     4021 gccctggctt cgaaagccag attcgcttca tcatccagga tatcgatggg ggcaaaatca
     4081 aagccgtgct ttctggccaa cgctatgtgg ctggcagttt tggagcgggg accgccataa
     4141 agcacgttgg tctccaccag ggtggcgtca agattgaggc aaaggtcttt gatcagggag
     4201 gcggggagga agttctggtt gccttcttcc ccaaagtgga gcttgatggc gatcttgcca
     4261 ttcagttcac cgctcagctt ggcatagatt ctgctcacgg cctgagggct gatctcgttg
     4321 gtgaaccaaa cggtggattg ctcgccagat gtgccctctt gtgaagcagg tttttgcggc
     4381 tcggctttca cgcaagcgca aggcaggagc attgttagca taataatgac ggcataaatc
     4441 aatttgcttt ccatgcaggt gtctccaaat cgctgggttg ctatttgagc agcatcatct
     4501 tgtaggtctt gctatatccc tgtcccgtaa ggcgatacat atacatgcca ctggctacgg
     4561 acctgcccgc gtcgtcctgg ccgttccaga ccgcctgatg gtttcctgcc ggcagctgtt
     4621 catctaccag caaacgaacg cgctggcctt tgaggttaaa aatctccagc ttcatatccg
     4681 tggactggct attggcaaac gcgatggtgg tgctgggatt gaagggattg ggataattgg
     4741 gatagagcgc caggctgggc agagcgggat tgagcaactg accatcggcc accgcggaac
     4801 ctgtgataac gcggatgtta tcgatggtcc agccgggatc ggtgagctcg atatggatgg
     4861 attcgtctgt gagtcggaac cgcaggtaat atgatccggg atagtcactt aggtccacat
     4921 actgccgttg ccaccagtcc ttgcgtcccg agctttccca gagagtggtc caggtggagc
     4981 catcggcaga gatttgcact ttcacgggat cgtgcacagg ctcggtgtag aggtgagaat
     5041 caaagctcag gagcggatct ccgctggcag gaatgctgat gggattgacg gtctgaatcc
     5101 agacgtcgca gttttgggcg tagaatcctc ttccgcccca gctgtctgta atcgcgtagc
     5161 cagaaacgct ttgttcgttt tggaccaccc aggggccttc aatttgccag ttggcggtgc
     5221 cgtcttccca gtcctctgag aagttgactg tggcgggact gagatcaaaa ctgcggtgat
     5281 tggcaccggg agtgagggtc acgctgccct ggtagggata atagccatcg gcgtggatgg
     5341 tgatgggata ctcgccttca aaggcttgat agacgtatgc tccgtttacg accagactgt
     5401 ccacccaggt attcccaagc acgatcagag cgggaatgtc gatcccaccg ctttggacgt
     5461 ggccactcag agtggcaggc tggcgcggag tgagtatcag gtcccgcggg gtccagttgg
     5521 tgttgttgat cgtcactgag ggcagcaccg tgtcaaaata tccctgcttt ttgcctatca
     5581 ccgtgtagtt tcccgtctgc aaagaccgat agtatctgcc attagtggca taggacagac
     5641 gcggtcgcag ccaatcggca tggtgctgct ggataatgac ttcagcttcc aggggctgat
     5701 ttgtgacgga atcctttaca atgccggtaa gcatcgcgct ggtgggcacg tcggaggaat
     5761 atggcaaagc ccggttgagc agccagcgca ctccattgct gcagcgctgg acggtgttga
     5821 ccatcagaag gctgtccggc tggagattgc gggtgccgca ctcgatcagg atctggatgg
     5881 tgccgtattg ctgatacatc cagtcgtgga aagcgccttt gcgactgaga ttgggcaaag
     5941 attcataggt ggccccacca gcttccttgg tgatctgggc cgctattccg gcagagatgg
     6001 cagcagcctg attgacgtcc ggagaggggc gcacctcttt ccaattgaag gggtagtagc
     6061 atttctcgct gaggttgccg gtgcgggagg aatgccagac gatggaatag acaaaccggt
     6121 attgctccgc caggttatag actgcctgca cttcgctttc gctcatggga gcggggcccc
     6181 ggtaataatc ccacacttcc aggcctcctg gctgcatgag ggtgtcgccc tgcacccagt
     6241 tgaagctgaa attacggttg atatccactc cgtcgatgtc gtatcccacc agattgctgt
     6301 agtcaaaaac accgttgttg ttattgtcgc gcttgttttt acgatatgag gtgtccaggt
     6361 tggcggtcac cacgttgtgg ccttccgggg tgaggctggg aatcaaccag atatccagct
     6421 ggttgatcca ctgattatag ggcagctgat agcggttggc cagaatctcg gcaatgttgc
     6481 tcatcgtgat ctgaacaccc aggacctctt cggcatgcac ctggcccacc aagagaatcg
     6541 cgggctcctg ttcgtcgttg ttgacgttgt cggaaatacg catggcatag atgggcaggc
     6601 cttcctgctg ggaaacaccg atctgcactt ttttggcaat atcgggatgc tgtgcttcat
     6661 gatcggaaag catctgagat atttgggcat aggtgtaata acaggtgggc agattctggg
     6721 ccccaagggc taagatagct gttagcagta tgataagcgc ggctaatcgc atttggactc
     6781 caagagattt gttatgtttt ccatttatta agcaatttcc agaccagcag aagggctaat
     6841 tggagcagag gctggttttg gattacgggt caagcgtttc catcaaaccg cgactctgtc
     6901 ccaccgctcc tctgttttga gtgtgataaa cctcaaagtg ctctaacgtc tacagcacaa
     6961 atcagatctg ccattgaagt gatgatgtag atcctctcag aatccttccc cggcatgctg
     7021 gtggcttatc tggagataaa atccagcacc tctccaccag gtatctgttt cactttgccg
     7081 ccgtcgttga tatcacgcat ggggccagct tcgatctctg cgctgtccgt gtagcctctc
     7141 agctcccaaa agccaggcac atagtggtcc atgagctcta tcctgacgat cgatttggcg
     7201 gatttgtagc cccagagata aggtgtaaaa gcacggatgg gaccgccata atcctcggtg
     7261 agcagttcgc cgtcaaattc ccaggccacg atggtctttt ccttgagtgc aatgtccagc
     7321 ggaatggaag tgtcatagac caggccgtga gaccagaagc ggatgtattt gcagctgtcc
     7381 cgcatgccca cttctctgag gaggtcggat agggccactc ctccccagat gcctctcaca
     7441 gaccatctgg tcacactggt gagcctggcg tcggctgtgg attttggcag cttcatcaat
     7501 tcctgccagc tgaaggtgcg gggttggtca cacaggccgg tgacttccag ggtccagctt
     7561 tcccggtcca atttgccggg atgaccttcc acccagaaga cgggggtgtt cattttggcg
     7621 agtttgtgca tggtgtttcc tttgttggga ttacagctca gagatgatag actgagaatg
     7681 atagtcaggc ctgacaacag gtatttaaca attgaggagc ggggacgcag cagggctttc
     7741 acgattccgt ccggggatgc accaggatct cttctacaac gtcgctgggg aggatggaag
     7801 ccgtaccgcg ctttgtggcc agctgttcgg ccgctctgcc aaggatgaga gaaccggcta
     7861 tggcactgag ggccatatgc tcgttctggg ctattcggga ggccacaatt ccggccagga
     7921 catctccgct tccacccgtg gcaaggccat cgtttccact gttggtgata aagacgtctt
     7981 cttcatgtct gatcaggctg aagtgacttt tcagcaggat ggtgacgggg ctttctggca
     8041 ggaaagaccg cagcttgccg atggggtcat tatacagatc gtccatattg atatcagcca
     8101 ggcggcaaaa ctcaccccaa tgaggggtta tgaccagttc ataacgatcc agcagaggca
     8161 gcatatcccg gttttccgag atgagattga gcgcgtcggc atcgatcacc agaggggtgg
     8221 catgctgttc cagcacgata ttcaggattc tgagggccca gggatcgcgt cccaggccag
     8281 gaccgatcag aatcacgctg gcgtccttcc agagctccgt gagctgttga gcttccggaa
     8341 ggccctcgtc atcctcaggg acgctcacat agagggcttc cgtgagcctg gaggcgtaga
     8401 ggctggcaag ctctttgcgg tgatagacat acacatagcc agctcccgtc cgcaaagccg
     8461 ctttggttgc cagcagactg gccccggtat agccttcccg gccgccaatg atcactactc
     8521 tgccatacat gcttttatag gctgtcgcaa aacggccgtc ggacggcagg tcctcataat
     8581 tggccagcca ggtgtgactg atatcttcat acatcgtttc attgatgccg atcggcactc
     8641 tctccagctt tccgcaatac agcttggccc ggccaaagaa atggccgggt ttgtaagtct
     8701 ccatggccag agtaagttcc gcttccacgc aaatcccggt gaggccatta tctccatgga
     8761 tgccggacgg aatgtccacg ctgcaaaccg gtgtcctgga ttcgtttatc cgctcgacaa
     8821 gctggatgac gtggtcgggc atcatagcct gaaagccggt cccatatatg gcatccacga
     8881 tcagggccgc gttgtccaga taaaacttga gctcgctttc tttttcgggc atggacatga
     8941 tcttgatttt gagcttttca cacagctcca ggttgtgctt ttgatccggc gaagctttct
     9001 cggaatcggt gaggatgatg agggtgttat aacccagaat gttgaaccag cgagcgatca
     9061 cagctccatc gccgccgttg tttccgggac cgcagacgat caggacatag ccgtccttgc
     9121 tcagcttgaa gtgatccagg atcttggtca cgcagccccg gcccgcgttt tccatcagga
     9181 ccagggacgg gataccaagt tcggtgatag ccttgccgtc cacggcccgc atttgttcta
     9241 cagtcaggat cgggatcata gatgctcctt tttatggtct tcggtgtgca gtttgatctc
     9301 gaaggtagtg ccttcattga cggtgctttc cagcactttg atatggcctt gatgatactc
     9361 ttcgatgata cgtttggcca ggctgagtcc cagaccccag ccccgctttt tggtggtcac
     9421 cccaggctcg aaaatcttct tccactggct tctgggtatg cccttgccct cgtcccggat
     9481 ctgcaagtag acccatttat ccttgcgggt ggcggtgagg atgatgtttc caccccggga
     9541 ggtcatggcg tccacgcagt tttttatcag gttttccagg gtccatttaa agagttccgc
     9601 gtccagcatc acgttcacgc cctcgatctt gctgatcaga tggatgtcta tccgcgatcc
     9661 caaatggggc atgcggtggc gaaagtattc caccgtctcg ctgaggatat catgcatggg
     9721 agcgggctgc agttttacct gagagcctac tttgccaaag cgggaggcga tgttgcgcag
     9781 gtgttccaga tcggtggtca tgaaatcgat gatctggccg gtgggcattt gtggttcaga
     9841 tccgggagca gtgctcttca gatagtcgat ccaccccatc aacgaagtaa taggcgtgcc
     9901 gaactggtgg gccgtctctt tggccaggcc aatccagagc gtgtcctttt cggtacgccg
     9961 cagcaggaag agcccatagc tggaaaaggc gatcagcagc acggcgatca gcaactccaa
    10021 caccacaaag ttgcggatct gggaaagcga tcgcggcagg gaaaagtaga tatagccaag
    10081 gctgtcggcc tcgctggcga tagggatctc tgccatcgcg tcaatgcggt cccggaggtg
    10141 ttgccggtca ttctctccga tctcggaata tctggctact tcaggaatat cgacgtttct
    10201 ccagaacagc gggaccttct gacggtccgt cacgatgatc gaataatcca ccttgctgat
    10261 aaattgctgc agcgataccg gtttggcata aaacgcgtag cccgtgacca tatcctcatc
    10321 ggtaagagga gtttggttca tgttggccat ctgctcctgc agcagcgcct gggatcgata
    10381 cgagagctcg gagtatagcg aatcctgtga aatccccact ttgttccaga acagaggcgt
    10441 gtaggcctcg tctgtgatga tcaccggaat ggggtttttt tgcataaact ccagactgat
    10501 ataatcccac agcgcttcct gaaagttgcg ctgggaagca aactggaagt agtgggtgcg
    10561 gacctcactg agcaggtcct catactgctc cgcctggcgt aagtagctat ccgtgtatgc
    10621 gatatactga gcaaagatac gtggcacgta ttcctgctcg cgtctggcat tgcggatcag
    10681 aatctcagta tagattgcga aggcgacaaa gatcaccacc gtgccgatga tgagatagag
    10741 acgcagggtc tgaaagcggt tagtgctttt tcgcgaggtc ccagactgcg tcgagttcct
    10801 gaagactggc ttcatggatg tccttcccgg agctttcata gtggtcttct atatagttga
    10861 aacgtttggc aaacttgcgg gtggtggctt taagcgcgct ttccgcgtcg atatgcagct
    10921 tgcgggccag attcactatg gtgaagagca ggtctcccaa ttcctcacgg atgttttcca
    10981 actgcccgga tgccagagcg gcgtgaaact cctcctgttc ctcttccagc ttggccagga
    11041 cgggttgcag gtctggccag tcgaagccca ccgaggcggc tttttcctgg gtccgctggg
    11101 cctgaatcag gcccggaagc gaacgtggaa tgccttccaa cacgctttta cgctctttct
    11161 tttcgcgctt cttgatgcgt tcccagttca ttttcactgc ttccgcacct tccacatggt
    11221 gatcggcaaa gacgtgcggg tggcgtctga ccagcttgtc gttgatcttg gacagcacct
    11281 cgcggatgtc aaaacggtct tcttcaacgg cgatctgggc ctggaagatg atgtgcagca
    11341 tgaggtctcc cagttcctca ctcagggaca cgtcatcctc gtcctcgatg gcttccacgg
    11401 cttcatagag ttcctctata aagttggcag tcaaggattt gggggtctgt ttgatgtccc
    11461 agggacatcc ggatacggga tcgcggagcc gggcgatgat ctggacgaga tcgtcaaatt
    11521 cctgcaaggg cttcctcctc gggagtttca gctttcagtg taacaggata tatcttgttg
    11581 aaaggtatat ggatggccac attgaccgcc gccagataga tccacacaaa gtagttccag
    11641 cgaatgctgc tgggcagaat gctggcaaag ataaacaaga tcagggagct cagccacatc
    11701 agattccagg cgcgctcctt tccatccgag cccctggcca gtttaaacag cctccaaatc
    11761 caatgcccat acagatacag gaaggccgcc aggatgatga tcccccagtt gccaaacagc
    11821 tccatcagaa agttgtgggg gttggtgatc cagccggtgc gcagcagggc atcagcgctc
    11881 atatagggct cgtaatttcc acctcccacg cccataaaac ctgtggacgc ggccatgagg
    11941 accgtctcgc cgatcaactg ctggcggatt ttgatcgagc tcatatacac cgaaccagat
    12001 tcggaaccga tgctactggt ctgatgtttg agcaccagca acaccagctt gatctctttg
    12061 tgaaaacgca gccagagcgc gcctcccagc atgagaacag ccagccccag gatcaatttc
    12121 tgccgcaaac ccactctcca gatgaagaac caggccagga aaaccgccat caccaaaatg
    12181 ccaatgcgag cgccctggat cgtcatcaat accaatacga tgaccgtgat cagggccgcc
    12241 agaattccca gccaccattt tccgctcaac tttggcaaaa agagcagaaa gggcgtgaac
    12301 aacatcatat aggcggctag gttgttttcg ttgtagaaag gcccggtggg cacatagtgc
    12361 caatcgccgt agagccgtga tgagggcaga tggtgaaaac tggtgagctc ccacagcgcc
    12421 gtgagcacat agagcagcaa aatcccgctc aaaaaccagg gggccagctt cagcagaccg
    12481 ggttttctca agagccggtc aaaggccata aacatcacgc tgtagcgtgc gatcagcaaa
    12541 gcgtagagca gcacttcctt gctgtccacg gcccagagat agctgagcag gttgtagata
    12601 aaccacaggg ccagaaacag caggggtctg gcattgaccg catcgatgag gcgggtctgg
    12661 ggatagatca gcacccaggc caggcagaat agcgccacca tgatcacggg agcgtagaac
    12721 agagcgggat gaaagggata gaactccacc agcgcgtaga ggccaaaggc catcaagatg
    12781 aggctggaaa gggatttatg ctgcttcaca tctggctgtc tgaatctgag gcggggcggg
    12841 atttcagggc tgagagcacg cggtcaatct cggctttgtg ggtattgatc acgttttctt
    12901 tgacgatagc cacaaaacag gccagcagaa aggccataaa ggtcacgata ctcaccagca
    12961 gagcgcgttt gggcttggag cgcagacctg ccatcgaggg cgggtcgagg atatcgaagc
    13021 tgggcatgtc acgcgcttca tccaacctgg cgatctcgta ttggggatag agatattcaa
    13081 acaccttttt ctggatctcc agattgagct tgagctgcgc gaactggagg ctgagatcgg
    13141 ggatgcggtc gatctggatc acatactccg gcatcagttt agagccgctg ccttccagct
    13201 ctttgacctt ggtcttcaga tagtcccggc ggctggccag ctcttccagc acgggagagc
    13261 cctctccata ctgcttgagc gccacgttgt attccatctc ggccttgtaa aaatccgaga
    13321 tactttcgct gtacagagtg atcagagagg cagtctgttc gtccagaccg atggcgcggt
    13381 tcttttgctg gaactgcttg actgccgtgg ccagggagtc tatcgtgagc ttggtgactg
    13441 ctacctgaga ctccagaaat tcgcgctgga gcttgctttt gctgccccgg ttgttgcgca
    13501 gatagtcttg cagctctgcc agatagaact ccgcgatctt tttggaaaag agcttgtctt
    13561 tggtctcgat ccgcaccgtg atcatcttgc tctcttcatc agcgttgatt ttggcgagct
    13621 tgctggatac cttgaagacg gccagttcca tggcttcgtc gcgcgggtta tccgtgagct
    13681 tgaagtattt gagcaggtca aactcgttta taacccgctc tctgaaactg cggctgcgca
    13741 ttatcgacac aaactccacg gccggatcgg tctggctacc ggacaaaaga ccgctgccga
    13801 tcatacccag caaaccgctg tccaatcctg acagtgacgc gttgtccgcc accggaacga
    13861 tcaccgtttt ggagacccag tgcttgggcg tcaccagcgc gtaaatgatg ctccccacgg
    13921 caaatacagc cgtgaagacg atcaccagcc atttatgctt cgcgatcacg tagaggaggt
    13981 cgaaaagacc cagttccttg cgcataatca ccacttcatc attattttgc gtttggcggt
    14041 cacaatgacg cgggtgggcg gaatctgtca actgatattt gccgcggggg aactgaagtt
    14101 ctgagcttat ggttgtatgt ttggggtata ggaatggtaa tcaatctcca aagaatggat
    14161 attcatggag
//
LOCUS       DAOSFN010000057        13917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3641_length_13917_cov_10.042346, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000057 DAOSFN010000000
VERSION     DAOSFN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3641_length_13917_cov_10.042346"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..1136)
                     /gene="rnhC"
                     /locus_tag="PLO35_05125"
     CDS             complement(243..1136)
                     /gene="rnhC"
                     /locus_tag="PLO35_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOH79082.1"
                     /translation="MRGIRVEEQSSIAYGEQLRLSSLGEKATLNLYWSARKGISFVIS
                     AKPGSGIKAILETIRDQTELDLQDQAPELAKHDWRLWIGSDECGKGDYFGPLVVCAFK
                     ASHEQTAELRALGVRDSKQLRDSQIIQIATSLYQQFPGRMSGIVLKPARYNQIMDDMK
                     SNSRNLNDLLAWQHCGVIQNLLDASQPVDGILVDQFSKSKKVAAAFKTKQPDLRVVER
                     SGAESDLAVAAASILARYQFLGAVKELSKRYKIDLPLGAGSNVDKAAREFVKLHGLDR
                     LNEVAKLHFKNTLRVQATPTP"
     gene            complement(1205..3427)
                     /locus_tag="PLO35_05130"
     CDS             complement(1205..3427)
                     /locus_tag="PLO35_05130"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOH79083.1"
                     /translation="MDTSIKIGIDGMHCASCSARVEKALAGLAGVKSAHVNLALEEAS
                     VEYDGRQLKPELFETTIRALGFSVREELEKSEDQLVLEARRARSRMWQAWGVAVIVSV
                     FMVSHMVFDQMLLGHLTDNWTIFILSLAAMIFPARRVYVSAWRSIRSGAANMDVLIAM
                     GTLASLLVWPLSFVVKGVESHAFSGIAAMILAFHLTGRYLESQARGKASEAIRKLMDL
                     GAKTAILYQDGVEKEVSIRKVQAGDILIVKAGLKVPTDGVILEGSANLDEALVTGESM
                     PVNRKPGDRVLGATLNLDGLIRIKAEKVGNDTFLAQVIRLVQDAQHSKVPIQLLADKV
                     TAIFVPVIIILALLTFAGWMLFPDVAQSISSFILRFLPLNSPETGLAAALMASIATLV
                     IACPCALGLATPTALMVGSGIGAKKGVLVRNGEALQTMKDVKAIVFDKTGTLTHGKPD
                     LIETITRDLDPDQALSIAAALESGSSHPLGQALVKAARSKNMKPLAIKDFSYHTGKGI
                     TASVAEVAYRIGSLEFIRENGLEPDGMRISAAASYASQVWLADHSRVLATFYIADGIR
                     PEAAEVVKTLKSMGIEPIMLSGDKQETAQAIANQVGIARFFAQALPEHKANMIKTLQK
                     EYGRVAMVGDGINDAPALKTADIGIAMGQGTDIAIEAADITIVRDDLRSLPLAVELSI
                     QTFRKIRQNLFWAFFYNLIAIPLAVLGILHPIIAEIAMASSSVTVVTNANLLRRKIKT
                     "
     gene            3599..>13917
                     /locus_tag="PLO35_05135"
     CDS             3599..>13917
                     /locus_tag="PLO35_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOH79084.1"
                     /translation="MKTSTKLLILVALLITASNLFATLRSVDPLILPQWHSSDPWNGR
                     CPGTAGNRANAGSHALALAKTMKYWAYPSSGTGNVSYVDDDYGDINSNFNPTINWDGM
                     SNTLVFQTTQRYIFMCGASVFTDYEQQNSSSTLANVRTSLINYFSYDPAMQIRSRGEF
                     TNFYWKAMIRAELDAARPVIYTVTLASGREVALLVDGYDEAGLFHINWSDVNVPDAWV
                     ELNTLSYMDEAIPETAQQMLTGIRPSLGPVNIDENFETDFSEFNWQFSGHANWTISTE
                     AAYYGTQSAKSGNINDNQTTSMFIQINVSQPDTISFFKKISCEAEPNHLYDHLAFLID
                     GVEQQRWSGDGAWEYHEYPVTPGVHEFRWTYNKDGASDYFGDCAWVDAVDLPEGSTPL
                     NPPRFVEAQLFAGNDVTVNWDPPMGSNPSLIGYRVYRNGTQIVQYNNPAQNFHNDYNL
                     ANGEYSYHLRAVYAEGISGPSNASSVEVEVPYAPTNLVAELSGVDTGHLTWLAPPLLR
                     DRALMGYKLYRDELLLAAIESPETLEYFDHNLAQGAYYYKISAIYASGESAFSNTAVL
                     AVGVPEPPLGLQAVVNGDDVSLSWQQVSDPQFLSGFKIYRNNSLIHQSTTPDLLGYTD
                     PDLANGSYSYYLRAVYGDTESGNSSTVTVSVEVPYPPTGLTATVNGDDVSLSWTNPLS
                     GRALTHYYIYRNAQVIAAVFNPNTLSYQDLNLPNGLYSYTISAFYSGVESAPSAPATA
                     LVEVLYPPTALAATVNLADVQLSWNIPATQGGLRAFLGYKVYRDGTLIATVMGGSNHQ
                     YTDVSLPNGSYNYAVSAYYNSGESSQALLNGVVVQVLYPVAVLNYQINSDDVQLWWSA
                     PASSPGRLSAPGRDPLEYRLYRDGALLSQSALTSYDDLDLANGLYQYWVTVIYPSGES
                     QPSPTVQLEIEVLYPPSNLSHTVNGANVNLSWDIAATSAGRGLLGYKIYRDESFIGSV
                     IANSYTDSALANGSYQYKVSALYAGGESSFTNTVTAVVEVLYAPQVLQYNVLDRNDVA
                     LHWQLPQSAPRALLAFKLYRDASLIYQGSELSFTDTDLADGVYVYQVTALYDSGESAP
                     SNTVSPFIEYPWPPTGLVTSVDQDDVSLNWDVLPGTQVLYQLYRDGVQIAELTTTTYL
                     DANLPNGVYSYHLTSSNASESGVSDPSLPVNATVEVLYGPSGLVYSLEDDDVLLSWNA
                     AAYTGGTRLLLGYDVWRDGAQIARTAELSYRDASLPNGVYQYYITAAYDTGNSNPSNS
                     VTVYLELLYAPSNLVQSVNGNDVSLSWSAAPSSGGLRSFNGYNLYRDGILIGNTTALS
                     YDDLNLANGTYQYYVKALYSSGESSSSNTVTAYVEVLYPPSNLSYAIADDDVTLSWNA
                     APNQERGLLGYRIYKDGALLDQTVQTTYTDASLPNGIYHYYVSALYDSGESLPSNTVT
                     VNLELLYPATNLTHSVAGDDVTLIWTAPVSSGGLRALLGYKVIRDGNQIALVNTTSYD
                     DLNLANGLYTYSVIATYDSGDALPTNAVTAMVEVLYAPSALSHTVIGDDVSLSWTAAP
                     TQSRAFQGYNIYRDGSLIGNTTDLNYSDPNLANGVYIYYVTALYGSGESSASNSVSAI
                     VEVLYPPTGLSYSVDDDTIILNWTAAVSSGGLRNLLGYDIHRDGLIIARTAVQSFSDP
                     NLPNGVYQYYITAAYDSGSSSPSNTVTVNLELLYPPSLLAHTVTGDDVSLSWTAAPAS
                     GGLRSFNGYNIYRDGNLIANTAALSYEDLNLANGVYQYYVTALYASGESAASNTATAL
                     VEVLYPPSNLSLQADADDISLTWSAPQTAPRGFLAYKVYRNGTFHAQTTATSWQDLNL
                     ANGVYSYQVSALYDSGESILIGPVSAEIDIPYPVTTLAASVNEDDVTLTWTLPSTTPP
                     RAFQGYFIYRNGSLHQVLANPSLSSWTDSGLANGDYSYYLIAVYDAGLSLPSNTVYIT
                     VNLVPDLPPPLALGLDMLAARDLRLSWQAPSPNVLSYHIYRNDTQIATTTGLGYDDLD
                     LPNGSYQYYVKALYAEGLSSASNRVVANIMIASPPSGTGDISASGNSVSFSWNAPGQG
                     EIGYIIYRNGIEVLYIPNPLSTSFTDSNLPNGNYLYQVAAVYPGVISSPVTIGSVRIL
                     VAYAPSGLVTSGSADTVTLSWTAPADLGLFSFYNVYRDGNFLAQSALASYTDTGLANG
                     SYGYYISSQYQELESPFSNVESYTVAVAYAPIALNASVNQDDVTLSWTGVSDLGGFIH
                     YRIYRDGILAGTSNLSSWQDNDLANGSYGYQITALYSFGESALSNSAAVEIELAYPAT
                     GLSYQLSGDDVQLSWTAPVNSGGLRGLLGYKVYRDGSFLAQTALPSYTDPDLVNGNYL
                     YYVTATYSSGDAAPTSTLNVTVELLYAPSGLAYSVVQDDVTLNWSPVSSAATNLLGYR
                     IYRNSSLIATTASTTYLDANLPNGSHDYVVKAFYPNGESDASNPVTAVIEVPYAPLNL
                     DYTVTDNDILLTWQAPPIQDRGLQGYQLYRNDVQIAFVTGLSYSDPDMPNGNYNYYLI
                     AQYSSGDSSPGNTITVPVFVPYTPTGVTVTGTGSGRTITWTRLNQMETHYRVYRNSML
                     IATIPASQPTSYSDPGISNGIHSYQVAAIYPGALISPLSESVQTTVMVAYPPTALNHN
                     FNTGDQNLTIVWTPPTEQTFLIGYRVLLYEDLTQVGQLLVDHPTTEATFTNTPNAMYQ
                     VYVQSRYSDGTDTIESLQAGPIYPWVHAAYPPSGLTGTIDGDDILLNWNPPVQTGLLL
                     DYVVYHRRDQGSWQLVNVLPTDTSYTLMNMQNGQYTIMVTARFGSTNNWSQSDYSNPI
                     TPSLEAPYPPSNLVATVEGNDVTLNWTGTAVPYGFQHYVILRNSTQLATTTNITYSDS
                     DLPNGNYSYQVYAVYGGGNSNPGNIQEVTVTQAYQPQSAELVLTGNNVLISWTAPSDQ
                     QFLTGYLVYRDSGLIATLGNQLSYTDSGLDNGDYGYGVIAVYGSTLSLVTNAGIVHVE
                     VPYPPADLVLEVADSSIEASWTATPDLGFFQHYTIFLNGNQIFEQQSTSYNFGVLPNG
                     NYIVGVEAVYQSTVSDRILSESQSLIVAYPARDLSAVVDSNHVSLSWQPPIDAFGLQE
                     YQIWRDSNLLSTLTALTYEDIAVPNGAHHYQIKTVYSGSASAAGNFALAEVLVAYPPS
                     NLSYNVSEENVQLIWQAPSDTGYLTGYKVYRDGAFFCFQNGPGMTDGPLHNGTYRYYV
                     TAVYADTLETTATNEVSVTIFMTYAAESAQAIQQESSFLISWEHPSSGNLPDFYKVWF
                     LTDGLQSDPAAWTYVGASDSTSIVDSIHGDLMEGDFLWAVIAVFGELEAQATFTDILP
                     VDLNPPIPTVTKLLGNYPNPFNPNTQIVFWLKQDGRVKLEIYNSRGQKVRTLHNGSMT
                     AGRHMLPWDGKDSSGRVLGSGIYLYKLEADG"
BASE COUNT         3099 a         4131 c         3592 g         3095 t
ORIGIN      
        1 atctttgttt gggacaccgg atctatctaa actgtcgtcc cgctgggact cgtaaggaat
       61 gataccacga ttattagttt tcgcaccagc ttcttcgcgg caatgacagc aaggccggcg
      121 cgctttacca tcatcctcct atcccgtcca tcacgcacgc gccacccatg ccaaagcacg
      181 cgataggcgt gcgataagcg tgcgataagc acgggatagt cgcaagagac aaaggggaga
      241 gcttatgggg ttggagttgc ttgcacccgg agcgtattct tgaaatgcag cttggccact
      301 tcattcaaac ggtccaggcc atgcagcttc acaaattccc gggcggcctt gtccacattg
      361 gatcccgcac ccaaaggcag atcgatcttg tagcgcttgc tgagttcctt cacggccccc
      421 agaaactgat atctggccag gatcgacgcc gcggccacgg ccagatcgct ttccgcgccg
      481 ctgcgttcca ccactctgag gtcgggttgt ttggtcttga aggcggctgc cacttttttg
      541 cttttggaaa actggtccac caggatgcca tccacgggct ggctggcatc cagcaggttc
      601 tggatcacgc cgcagtgttg ccaggcgagg aggtcgttga ggttgcggct gtttgatttc
      661 atatcatcca tgatctggtt gtagcgggct ggtttgagga cgatgccgct catgcggcca
      721 ggaaactgct ggtagagaga ggtggcgatc tggatgatct ggctgtcgcg cagctgtttg
      781 ctatctctca ctcccagggc cctgagttcc gcggtctgct catggctggc tttgaaagca
      841 cagaccacca gggggccgaa gtagtctccc ttgccgcatt cgtcggagcc gatccaaagc
      901 ctccagtcgt gttttgccag ctccggggcc tggtcttgca ggtccagctc cgtctggtcc
      961 cggatggttt ccagaatggc cttgatgccg ctgccgggtt tagcgctgat cacgaacgag
     1021 atccccttgc gggccgacca atagaggttg agggtggcct tttcccccag gctggagagc
     1081 cgcagttgtt ctccgtaggc gatggaagac tgctcttcca ctctgatgcc ccgcatagcc
     1141 agttggggca ggatttcggc gagtttggct tcgatgtgtt tatccagttt cagattctcc
     1201 tgatctaggt ttttatcttg cggcgcagta aattggcgtt tgtgaccacg gttacggaag
     1261 aactggccat ggcgatctcg gcgatgatgg gatgcaggat gcccagcacg gccagaggga
     1321 tggcgatgag gttgtaaaag aaggcccaaa ataggttttg gcggatcttg cggaaggtct
     1381 ggatggagag ttccacggcg aggggaagac tgcgcagatc gtctctcacg atcgtgatat
     1441 cagccgcttc gatggcgatg tcggtgccct ggcccatggc gattccgata tcggcagttt
     1501 tgagggcggg agcgtcgttg atgccgtctc ccaccatggc cactctgccg tattcctttt
     1561 gtaaggtctt gatcatattg gccttatgct caggaagcgc ttgggcaaaa aagcgcgcga
     1621 tccccacctg attggcaatc gcctgagcgg tttcctgttt gtcgccgctc agcataatcg
     1681 gctctatgcc catggatttg agcgtcttaa ccacttccgc ggcctcgggc ctgatgccat
     1741 cggcaatata gaaggttgcc agcactctgc tgtggtcggc cagccatacc tggctggcat
     1801 aggaagccgc ggcggagatt ctcattccat cgggttccag gccgttttcc cggatgaatt
     1861 ccaggctgcc gatgcggtat gccacctcag ctaccgaagc tgtgattccc ttgccggtgt
     1921 gatagctgaa gtctttgatg gccaaaggct tcatgttttt ggaacgggcg gctttaacca
     1981 gggcctggcc cagcggatgc gatgatccgg attccagggc ggccgcgatg ctcaaagctt
     2041 gatcgggatc gaggtcccgg gtgatcgttt cgatcaaatc aggcttgccg tgagtgagcg
     2101 tgccggtctt gtcaaacacg atggccttca cgtccttcat ggtctgcaga gcttcgccgt
     2161 tgcgtaccag gacgcccttt ttggccccaa tcccgctgcc caccatcaga gccgtgggtg
     2221 tggccaatcc cagagcgcag gggcaggcaa tcaccagagt ggcgatcgag gccatcaaag
     2281 cggctgccaa tccggtttcg ggagaattga gcggcaagaa tctcaggatg aaagaactaa
     2341 tgctttgcgc cacatctgga aagagcatcc agccggcgaa ggtgagcagg gccagaatga
     2401 tgatcaccgg cacgaaaatg gcggtaactt tatctgccag aagctgaatt ggcactttgg
     2461 aatgctgggc atcctggacc agtctgatta cctgggccag aaaggtgtcg ttgcccactt
     2521 tttcggcttt gatcctgatc aaaccgtcca gattgagggt ggcgcccaag acccggtctc
     2581 ccggcttgcg gtttaccggc atcgattctc ccgtcaccaa agcttcatcc agattggcgc
     2641 tgccttccag gatcactccg tcggtgggaa ctttcaggcc cgcctttacg atcaaaatat
     2701 caccggcctg gaccttgcgg atggatactt ctttctccac gccgtcttga tagaggatag
     2761 ccgttttggc gcccaggtcc atcagtttgc ggatcgcctc ggaggccttt cctctggcct
     2821 ggctttccag atatctgccg gtgaggtgga aggccagaat catggccgcg ataccgctaa
     2881 aagcatggga ttcaacgcct ttgactacaa aggaaagggg ccacaccagc agtgaggcca
     2941 gggtgcccat ggcgatcagc acgtccatat tggcagctcc cgatcggata gaacgccagg
     3001 ctgatacata gacgcgacgg gccgggaaga tcatagccgc caggctgagg ataaagattg
     3061 tccagttgtc ggtgaggtgg cccagcagca tttggtcaaa aaccatgtgg ctcaccataa
     3121 atacactcac gatgacggcc actccccagg cctgccacat gcggcttctg gctcggcggg
     3181 cttccaatac cagctggtcc tcactctttt ccaattcctc acggacgctg aatcccagcg
     3241 ccctaatggt ggtctcaaag agctcaggtt tgagctgtcg gccgtcgtat tccacgctgg
     3301 cctcttccag ggctagattc acgtgggcgc ttttcactcc cgccaaacct gccaaagcct
     3361 tttccactct ggcactgcag gacgcgcagt gcatcccgtc gatgccgatc ttgatgcttg
     3421 tatccatgct tgattcctta taatatcata cctacaagat atgcgtaatg gctcatgatg
     3481 acaagcacgc tgtttccgat gaggaaatcg gcccgggata gaacattcct caaacttggc
     3541 actaaacttg cattgaaatc tcctgaacag acataagtcc gtttgaccag aggttcaaat
     3601 gaaaacaagc accaaactgt tgattcttgt cgcgctgcta attaccgcgt caaacctttt
     3661 tgccaccctc cgctcggtgg atcccttgat cctgccgcaa tggcacagca gcgatccctg
     3721 gaatggccgc tgcccaggca ccgctggaaa ccgtgccaac gcgggttctc atgctttggc
     3781 cctggccaag acgatgaaat actgggccta tccctcctcg ggaacgggaa acgtatccta
     3841 tgtggatgat gattacggcg acatcaactc caatttcaat ccgaccatca actgggacgg
     3901 gatgtccaac actctggtgt ttcaaactac tcagcgctat atatttatgt gcggcgcttc
     3961 ggtgtttacg gattatgagc agcaaaattc cagctccact ctggccaatg tccgcacttc
     4021 tctgatcaac tatttcagct atgatcccgc catgcagatc aggtcccgcg gggaatttac
     4081 caatttctac tggaaagcaa tgatccgggc cgaactggat gccgcccgtc ccgtgatcta
     4141 caccgtcact ctggccagcg gccgggaagt agctctgctg gtggacggtt atgacgaagc
     4201 gggcctcttt catatcaact ggagcgacgt caatgtgccg gacgcctggg tggagctcaa
     4261 caccctcagc tatatggatg aagctattcc tgaaacggcc cagcaaatgc tgaccgggat
     4321 ccgtcccagc ctggggcctg tcaatatcga cgagaacttc gagacggatt tcagcgaatt
     4381 caactggcag ttttccggcc acgccaattg gaccatttcc accgaggcgg cctactatgg
     4441 cactcaatcc gccaaaagcg gaaacatcaa cgacaatcag accacctcaa tgtttatcca
     4501 gatcaatgtc tctcagccgg atacgatcag cttctttaaa aagatctcct gtgaagcgga
     4561 acccaatcat ctctacgacc atctggcctt cctgattgat ggagtggaac agcagcgctg
     4621 gagtggagac ggtgcctggg aatatcacga gtatcccgtc acgccgggag tgcatgaatt
     4681 ccgctggacc tataacaaag acggggccag cgactatttt ggagactgcg cctgggtgga
     4741 tgcggtcgat ttgccggaag gcagcacccc tttgaatcca ccgcgttttg tggaggctca
     4801 gctttttgcc ggaaacgatg tgacggtgaa ctgggaccct cccatgggca gcaatccttc
     4861 cctgatcggc taccgcgtct atcgcaatgg cacccagatc gtgcaataca acaatcccgc
     4921 ccaaaacttt cacaacgact acaacctggc caatggagaa tacagctacc acctgagagc
     4981 ggtttacgcg gaggggattt ccgggcccag caacgcgagc agcgtcgagg tggaagtgcc
     5041 ttacgcgcct accaatctgg tggctgagct cagcggtgtg gataccggcc acctgacctg
     5101 gctggcccca cccctgctaa gagacagggc cctgatgggt tacaagctct atcgggatga
     5161 actgctgctg gccgccatcg aaagtcccga gacactggaa tactttgacc acaatctggc
     5221 ccagggggcc tattactata agatcagtgc catttacgct tccggggaat cagctttcag
     5281 caacaccgcc gtcctggctg tgggagtgcc ggaaccgcct ctgggcttgc aggctgtcgt
     5341 gaatggcgac gatgtctccc tcagctggca gcaagtgtcc gatccgcagt tcctcagcgg
     5401 tttcaagatc tatcgcaaca acagcctgat ccaccaatcc accactccgg acttgctcgg
     5461 ctataccgat cccgatctcg ccaatgggtc ctactcatac tatctgaggg ctgtgtatgg
     5521 agatacggaa tcgggaaatt catccacggt gacggtaagc gtggaagtgc cctatcctcc
     5581 aactggattg acggccactg taaacggcga cgatgtgtcg ctatcctgga ctaaccccct
     5641 ctctggcaga gcattgactc attattatat ctaccgcaac gcgcaggtga tcgctgctgt
     5701 cttcaatccc aacaccctca gctatcagga cctcaatctc cccaatggcc tctatagcta
     5761 cacgatctca gctttctact ccggggtgga atctgccccc tcagctcctg ccacggctct
     5821 ggtggaggtg ctctatcctc ccaccgcttt ggctgccacc gtcaatctgg cagacgtaca
     5881 gctatcctgg aacatcccag ccacccaagg tggattgagg gcgtttttgg gttataaagt
     5941 ctatcgggat ggtactttaa tcgccaccgt catgggtggt tccaaccacc agtacaccga
     6001 tgttagcctc cccaacggca gctataacta cgccgtgagc gcgtattaca acagcgggga
     6061 atcctctcaa gctctcctca acggtgtggt ggtgcaggtg ctctatccgg tggcggttct
     6121 gaactatcag atcaactcgg atgatgtgca gctgtggtgg agcgcgcccg cttcctctcc
     6181 gggacgcctt tccgctcctg gacgcgatcc tctggaatat cgtctctacc gcgatggagc
     6241 attgctgtcg caaagcgcgc tcaccagtta tgacgatctt gatctggcca acggcctcta
     6301 tcagtattgg gttacggtga tctatcccag cggagaatct cagcccagcc ccaccgtcca
     6361 gctcgagatc gaagttttgt atccccccag caacctatcg cacacagtga acggcgctaa
     6421 cgtgaacctc agctgggata tagctgccac cagcgctggc agaggactgc tgggctacaa
     6481 gatctaccgg gacgaatctt ttatcggtag cgtgattgcc aattcctaca cggacagcgc
     6541 tttggccaac ggcagctatc aatacaaggt gagcgctctc tacgcgggtg gggaatccag
     6601 cttcacaaac acggtgacgg ctgtcgtgga ggtgctctac gctccccagg tgctacaata
     6661 caacgtgctg gaccgcaacg atgtagccct gcactggcag cttcctcagt ccgcgccccg
     6721 cgccttactg gccttcaagc tctatcggga cgccagtctc atttatcaag gaagtgagct
     6781 cagctttacg gataccgatc tcgcggacgg cgtctatgtg tatcaggtca cagcccttta
     6841 cgacagcgga gaatcggctc cctccaacac cgtgagcccc tttattgaat acccctggcc
     6901 ccccacaggc ctggtgacca gtgtcgatca agacgacgtg agcctcaact gggacgtcct
     6961 gcctggcacg caggtgcttt atcagcttta ccgggacgga gttcaaatcg cggaactgac
     7021 cactacaacc tatctggacg ccaacctccc caacggagtt tacagctatc atctgaccag
     7081 ctcaaacgct tcggaatccg gggtttcaga tccgtctctg ccggtaaacg ccacggtgga
     7141 agtgctctat gggccttcgg gactggtcta cagcctcgag gatgacgatg tgctgctcag
     7201 ttggaatgcc gcggcttaca ctggcgggac cagactcctg ctgggatatg acgtttggcg
     7261 ggatggggct cagattgcca ggacagcgga gctcagctat cgcgacgcca gccttcccaa
     7321 tggcgtctat cagtattata tcacagccgc ttacgacact ggaaactcca acccctccaa
     7381 cagcgtaacg gtctatcttg agctgctcta tgctccctcc aatctggtcc aaagcgtgaa
     7441 cggcaacgac gtcagcctca gctggagcgc cgctcccagt tccggaggcc tcagaagctt
     7501 caacggctat aacctctacc gggacggcat cctgatcggc aataccacag cgctcagtta
     7561 tgacgatctc aacctggcca acgggaccta tcaatactat gtaaaagccc tctactccag
     7621 cggagagtct agctccagca ataccgtaac cgcctatgtg gaagtgctgt atcctcccag
     7681 caatctgagc tacgcgatcg ctgacgatga cgttactttg agctggaatg ccgctcccaa
     7741 tcaggaacgg ggcctgctgg gctaccgaat ctataaagac ggcgctttgc tggatcagac
     7801 cgtccagacc acctataccg atgccagcct gccaaacggg atctatcatt actatgtgag
     7861 cgcgctgtat gacagcggag aatcactgcc gagcaacacc gtgacggtaa atctggaact
     7921 gctgtatccc gctaccaacc tcactcactc tgtagctgga gacgatgtta ccctgatttg
     7981 gaccgcgcct gtttcatcag gtggattgag agctttgctg ggctacaagg tgattcgcga
     8041 cggcaaccag atcgcgctgg tcaacactac cagctatgac gatctcaatc tggccaacgg
     8101 tctctatacc tacagcgtga tcgccactta cgacagcgga gacgctctgc ccaccaacgc
     8161 ggtaactgct atggtggaag tgctttacgc tccttcggca ttgagtcaca ccgtcattgg
     8221 cgacgacgtc agtctgagct ggaccgccgc gcccactcag tcgcgagcct tccagggcta
     8281 caatatctat cgggatggca gtctgattgg caataccacg gacctcaact acagcgatcc
     8341 aaacctcgct aacggcgtct atatctacta tgtaacagcg ctttacggca gtggagaatc
     8401 ctctgcttcc aactccgtga gcgctatcgt ggaagtcctg tatcctccca ccggactcag
     8461 ctacagcgtt gatgacgata ccattatcct gaactggacc gcggccgtca gctctggcgg
     8521 actgagaaac ctgctgggtt acgacatcca cagagacgga ctaataatag ccaggaccgc
     8581 cgtacaaagc tttagcgatc ccaatctgcc caacggcgtg tatcagtatt atatcacagc
     8641 cgcttacgac agtggcagtt caagcccctc caatacggtg acggtgaatc tggaactgct
     8701 ctatccgcct tccctgcttg cacatacagt cactggagac gacgtcagcc tcagctggac
     8761 ggccgctccc gcatcgggag ggcttagaag cttcaacggt tacaacatct atcgggatgg
     8821 caatctgatc gccaacaccg ctgccttgag ctatgaggac ctcaacctcg ccaacggcgt
     8881 ctatcaatac tatgtaacag ccctttacgc cagtggggaa tcggctgcca gcaacaccgc
     8941 caccgccttg gtagaagtgc tttacccacc ttcaaacctc agtctgcaag ccgacgcgga
     9001 cgatatcagc ctgacctgga gcgctcctca gaccgctccg cgcggatttc tggcctacaa
     9061 ggtttaccgc aacgggacat tccatgccca gacgactgct acaagctggc aggacctcaa
     9121 tctggccaac ggcgtctata gctatcaggt ttcagcgctt tacgacagcg gcgagtctat
     9181 tctgatcggg cccgtaagcg ctgaaataga catcccttat cccgtcacca cgctggcggc
     9241 ttctgtaaat gaagacgacg taactctcac ctggactttg ccttccacta ctccccccag
     9301 ggcttttcag ggctacttta tctatcgcaa cgggtctttg catcaggtgc tggccaatcc
     9361 cagcctcagc agctggacag acagcggcct ggccaatggc gattacagct actatctgat
     9421 tgccgtatat gacgctgggc tatccctccc ctcaaacacg gtctatatca cggtcaacct
     9481 tgtgccggac ctacccccac ctttggctct tgggttggac atgctggcgg ccagagacct
     9541 gcgactgagc tggcaagcac cgtcccccaa tgtgttgagc taccatatct accgcaacga
     9601 tacacagata gccacaacca ccgggctcgg ttatgacgac ctcgatttac ccaacggatc
     9661 atatcaatac tacgtgaaag ccctctacgc ggaggggctg tcttccgctt ccaaccgtgt
     9721 ggttgccaac attatgattg cctcgccgcc ttccgggacc ggagatatca gcgccagcgg
     9781 taacagcgtc agcttcagtt ggaacgctcc gggccagggt gagataggtt atatcatcta
     9841 ccgcaacggg atagaggtcc tctatatccc caatcccctg agcaccagct tcacggacag
     9901 caatctgccc aatggaaact acctgtatca ggtggcggcc gtgtatccgg gtgtgatttc
     9961 cagccctgtc acgataggca gcgtcaggat cctggtggcc tacgctccca gcggactggt
    10021 cacaagcggt tccgctgaca cggttacgct cagttggacg gccccggctg acctgggcct
    10081 cttcagtttt tacaatgtgt accgcgatgg caacttcctg gcccagagcg ctttggccag
    10141 ctatacagac acgggattgg ccaatggcag ttacggctat tatatcagtt ctcagtatca
    10201 ggaactggaa tcacctttca gtaatgtcga aagctatacg gtggcagtcg cttacgctcc
    10261 cattgccttg aatgccagcg tgaatcagga cgatgtgacc ctctcctgga ctggggtaag
    10321 cgacctgggt ggcttcatcc attaccggat ctacagggat ggaatcctgg ctggtaccag
    10381 caaccttagc tcctggcagg acaatgactt ggccaacggc agctacggct atcagatcac
    10441 ggccctctac agctttggag agtccgcgct gtcaaacagc gccgcggtcg agatcgaact
    10501 ggcctatccg gccacaggcc tcagctatca gctcagcggc gacgacgtcc aactgagctg
    10561 gaccgctcct gtaaacagtg gaggattgcg tggcctgctg ggttataagg tctatcggga
    10621 tggcagcttc ctggcccaga ccgctctgcc ttcctatacc gatccggacc tcgtcaacgg
    10681 caactatctc tattacgtga cagccacata cagcagcgga gatgcggccc ccacttccac
    10741 cctcaacgtg acggtggagc tgctctacgc tcccagcggt ctggcctaca gcgtggtcca
    10801 ggatgatgta accctgaact ggtcgccggt ctcatccgcc gccacaaacc tcctgggata
    10861 caggatttac aggaattcca gcctcatcgc caccacggca agcactacgt atctggatgc
    10921 caacctgccc aacggctctc atgactacgt ggtaaaagcc ttttatccca acggggaatc
    10981 ggatgccagc aaccctgtaa ccgctgtcat agaagtgcct tacgctcccc tgaacctgga
    11041 ttacaccgtt acggacaacg atatcctcct cacctggcag gcgcctccta tccaagatcg
    11101 cggcctgcaa ggctatcagc tctaccgcaa cgacgtccag atcgcctttg tgacaggcct
    11161 cagctattcc gatccagata tgcccaacgg caattacaac tactatctga tcgcacaata
    11221 cagcagcgga gattcgtctc cgggaaacac gatcacggtg ccggtgtttg tgccatatac
    11281 tcctaccgga gtgacagtta caggaacagg aagcggccgt acgatcacct ggacccgcct
    11341 caaccagatg gaaacccact accgcgtcta tcgaaacagc atgttgatag ccaccatacc
    11401 tgcttcacaa ccaaccagtt acagcgatcc tggaatctca aacgggattc acagttatca
    11461 ggtggcggcc atctatccgg gggccttgat ttccccgctc tctgaatcgg tccagactac
    11521 tgtgatggtg gcctatcctc ccaccgcttt gaaccataac ttcaatacgg gagaccagaa
    11581 tctgaccatt gtttggactc ctcccaccga acagaccttc ctcattggct accgggtatt
    11641 gctgtatgaa gacttaacgc aggttggcca actgctggtg gatcatccca ccacagaggc
    11701 tacgttcaca aacaccccca atgccatgta tcaagtctat gtccagagtc gctattcaga
    11761 cggcacagac actatagaat cattgcaggc gggcccaatc tacccttggg tccatgccgc
    11821 ctatccgccc agtggcctga ccggaactat cgatggggac gacatcctcc tgaactggaa
    11881 cccgcccgtc cagacaggtc tgctgctgga ctatgtagtt tatcatcgca gagaccaggg
    11941 ctcctggcag ctagtcaacg ttcttccaac ggataccagt tacactttga tgaacatgca
    12001 aaacggccag tacacgatca tggtcaccgc tcgctttggg tccaccaata actggagcca
    12061 atcagactac agcaacccca taaccccctc tctggaagca ccataccctc ccagcaatct
    12121 ggtggcaacg gtggaaggaa atgatgtgac cctcaactgg acaggtacgg ccgtccctta
    12181 tggtttccag cattatgtga tcctgcgcaa ttccacccag ctggccacca cgaccaatat
    12241 cacttacagc gattcagacc tgcccaacgg aaactacagc tatcaggtat atgccgtgta
    12301 tggaggaggt aactccaacc ccggcaacat tcaggaagta actgtaacgc aagcctacca
    12361 gcctcagagc gctgagctgg ttctcacggg aaacaatgta ttgataagct ggaccgcgcc
    12421 atccgatcag caattcctca ccggctactt ggtgtatcgg gacagcggac tgatcgccac
    12481 tttgggtaac caactcagct ataccgacag cggacttgac aatggggatt acggttatgg
    12541 cgtgatcgct gtctatggca gcaccctttc tctggtcacc aatgccggga tcgttcatgt
    12601 ggaagtgccc tacccgcctg cagatcttgt gcttgaggta gcggacagct cgatcgaggc
    12661 ctcatggaca gctactccgg acctgggatt cttccagcac tatactatct tcctgaacgg
    12721 caatcagatc ttcgagcagc agagcacttc ctacaacttt ggcgtcctac ccaatggaaa
    12781 ctacatagta ggcgtggaag cggtgtatca atccaccgtc tcagacagga tactctctga
    12841 aagccaaagt cttatcgttg catatccagc ccgtgatctg agcgcggtgg tggacagcaa
    12901 ccatgtgagt cttagctggc agccacccat tgatgcattt ggccttcagg aatatcaaat
    12961 ctggagagac agcaacctgc taagcaccct cacagctctc acatatgaag atatagcggt
    13021 ccccaacgga gctcatcact atcagatcaa aacggtatat tccggctcag ccagcgctgc
    13081 tggaaacttt gccttagccg aggtcctggt ggcctatcct cccagcaatc tgagctataa
    13141 cgtcagcgaa gaaaacgtgc agctgatctg gcaggcgccc tcagatacgg gatacttgac
    13201 tggctacaag gtctatcgcg atggcgcttt cttctgcttc caaaacggtc ctggcatgac
    13261 ggacgggcct ctgcacaatg gaacttaccg ctactatgtg accgctgtgt atgccgatac
    13321 cctggaaacc actgccacca atgaagtaag cgtgacgata ttcatgacat acgccgcaga
    13381 atctgctcag gccatacagc aggaaagcag cttcctgatt tcctgggagc atccctccag
    13441 cggcaatctg cctgatttct acaaggtctg gttcctcacg gatggcctgc aaagcgatcc
    13501 agccgcctgg acctatgtgg gggccagcga ttccaccagt atcgtggata gcatccacgg
    13561 cgatctgatg gaaggggatt tcctctgggc tgtcatcgcc gtatttggag aactggaagc
    13621 gcaagcgacc ttcaccgata tcctgccagt ggacctcaat ccacccattc caaccgtcac
    13681 caagctgctg ggtaactatc ccaatccctt taaccccaac acccagatcg tcttctggct
    13741 caaacaggat gggcgagtca agctggaaat ttacaactcc cgcggccaga aggttcgcac
    13801 cctgcataat gggtctatga cagccggacg gcacatgctt ccctgggatg gcaaagactc
    13861 ctccggaaga gtcctgggct ctggaatcta cctctacaaa ctggaggccg acggcta
//
LOCUS       DAOSFN010000058        13864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3668_length_13864_cov_9.890434, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000058 DAOSFN010000000
VERSION     DAOSFN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_13864_cov_9.890434"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1047)
                     /locus_tag="PLO35_05140"
     CDS             complement(64..1047)
                     /locus_tag="PLO35_05140"
                     /inference="COORDINATES: protein motif:HMM:NF018378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HOH79085.1"
                     /translation="MVIAIVLHAFIWQYAETRLLKLGEELSQLVRGRPVNQVQRLDSD
                     GIPYQTYADGSQHYNPLFVASVANQLYEELPDSLARARFIKLSDWLGKAMSQKGKALW
                     LSYDFDYPKFEMKAPWHSALAQAEAMTVCARRWKLTRERVWLHRSQALAQSLKPPSEL
                     TITMSINTMEDPGHKRRDGLWFMEYPGDKAPYVLNGHAAVLLELHRTWELTSDPGLEK
                     LFDRGFRGLREAIRRFDKKGFSLYSAEGELAGRAYHQKHIKQLMELIQIRPDPELDGY
                     LDRWQRSDGYPVIVQLFFNPRPNRILAFGISLILLWAQLYILLRIVSRGPH"
     gene            complement(1065..1931)
                     /locus_tag="PLO35_05145"
     CDS             complement(1065..1931)
                     /locus_tag="PLO35_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOH79086.1"
                     /translation="MELRFSQKPLLILHSQFSTLNSPFSMLSYSFLVRALLAALLSSV
                     SLAMFSPFVTLRRISYLGEALSHMAFAGIAIALLVGLDIQFTALAFVVLVALAIGWLS
                     QKHKLEEANTITIFLSVSMALGIILINLKKSYSFDLASYLFGNVLLVSQADLIRVGIL
                     ALLSLGFILLLYKELFYLSYNSEMAGFYRVPVVLVNRLFLALLAANIVINLKAAGIIL
                     VSAQLILPAATAFNLARRLTIVIVLSALVAVIAALGGFALSWWLDLPTGANIVMLEFV
                     LFILSLVIRRRF"
     gene            complement(2147..2875)
                     /locus_tag="PLO35_05150"
     CDS             complement(2147..2875)
                     /locus_tag="PLO35_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79087.1"
                     /translation="MIRIKDLNHAIAGIPILEDIQLEIAEGEFVAIIGPNGAGKSTLI
                     KLILGLIPLQQGSISIDGEPHLQWLKTHAMGYLPQREEYDRRFPARALDIVMLGLAGE
                     LKLGQRFSRQQRDRAQEAMRTTGCQEQARKPIGGLSGGEMQRVLLARAIVGGSKYLIL
                     DEPEASVDQPGVRSFFELLKELNKQGRTIITISHDLHTLTDYCSFLVCLNRRLHCHDH
                     AEMINAELIHKTFGDAVRIIEKRY"
     gene            complement(2872..3828)
                     /locus_tag="PLO35_05155"
     CDS             complement(2872..3828)
                     /locus_tag="PLO35_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOH79088.1"
                     /translation="MKNNCSQRSAWIRVIIMVTLIAGLALLGCRPRVKDDRPLIVTSI
                     HPYKLLIQEIVGDSIQVRSLIPPKASPHTWTAKPADMKSLDDAKLLVINGLGLETELT
                     GAFEERSAKLIDVSKLLKLEDQAQVQADSTLAVETDHAHAGMNPHIWLSPQLMLRAVI
                     MLSDELQTRFPSQAATIRANTSRMINDLAAVHRQITSERAAIAVPILITYHDSFHWFA
                     KDYDVQIMGTVQSSPGQEPTARELTLLGAVIKTNKIKAIFVEPQMDKRSAKVLADEFG
                     LALLELDPLGQSHGSARLIDLIWSNWERMKLSWNQEKPSVKP"
     gene            complement(3926..5404)
                     /locus_tag="PLO35_05160"
     CDS             complement(3926..5404)
                     /locus_tag="PLO35_05160"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOH79089.1"
                     /translation="MKIDLLRKLPEHMDTVVLLLDDKSKIESIEYLPESYKELVTAYI
                     KSEGYNPDYAALKSFQTVQDGHRANVVICGAGDPAALTPDRLRKLFADITRAAIRFNA
                     KLIYLFPGFQIPIGDVQFGHVLAEAALLTEYKFTKYLSTDKQHAIEALHLAVNLKNTR
                     HINRGILEGRIFAETTNMARQLVNEPANVIYPETLAEAAKKAALQYGFNIEIYTADKI
                     RRIKMDAFLAVGKGSKAEPRLIIMRYNGNPDAKNKTIGLIGKGLTYDSGGYCIKTPQG
                     MVNMKNDMAGAAAVIGAMSAAATLKLKVNLVGIVAACENMISGDAYHTGDLIRSMAGK
                     TIEVINTDAEGRLTLVDAIHFAISKEHVDKVLDIATLTGAAVGALGNQISAVVTNNDA
                     WLDKLKAASDFSGEKIWQLPAHEDYKELIKSQIADLKNTGGPFAGTITAALFIREFVE
                     DKPWLHIDIAGTALRDGESGMYSVGATGVGVRLITQLLREME"
     assembly_gap    5427..5526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5527..7766)
                     /locus_tag="PLO35_05165"
     CDS             complement(<5527..7766)
                     /locus_tag="PLO35_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79090.1"
                     /translation="MSHYKTEGQRDRGVEKTVIAILALLLLSSFVTLNAYSFGQNKVN
                     AVDSNWSMIRSMHFDVYFPAGNDDFGRLATLMAEETYYALKQDLRFPLSSRVPLIIYG
                     TKTEFQGTNIIYPLLTEGIGGFTESLRNRVVIPFEGSYAKLEELMAHELTHAYTNAIE
                     RGSSLVSSLRPASFPFWFSEGLPEFLSIGGEDDYNNMFILDMVVNESLPPLEYSDGYL
                     AYRLGESFLSYIAEVWGREKVSDYYFALRSSRGTDEATKKVFGMELEELESRWRYHLK
                     RLYFPAVNQHGTPKEDMEQRTFSARDGSYFNYMPRFSPDGQNYVYFSNQGARYSVWMS
                     GTHGLSRPRKLITGEATGKMEEFYYFRANLSWFPDSRRVAFGAKNSVGDVIHILDTHT
                     EKIVSSISIPELRGIYELDVSPDGTRILISGQKDMQSDIFVYDIASKTLGRLTDDLYW
                     DAQARWAPDGQSIVFASERILNPEGKRRGFFANYRSAIYQLHLASGELTQMSHEPGNC
                     SYPMLDAGGKKLLYISSDQGVANFRVLDLEAGTKAAVTNVLAGAYSGDLSRDGRYLLV
                     SNYFNGAWDIYFSYNPLDSLEYQPVMAPQPYSGKDDMWDGVDLSQLDLFGRRPKARPK
                     RINPAAQYDARRPLIGEEPKFEFTRQDSLLFFQDFSYDDRPTQPGPKLPRTEKYRSGF
                     AIEHLWGGLAYSSSVGALGYVELGLADIMGNHGIGINAGFSGILEENDILLSYLYLKK
                     RSDFGIG"
     gene            complement(7784..8686)
                     /locus_tag="PLO35_05170"
     CDS             complement(7784..8686)
                     /locus_tag="PLO35_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOH79091.1"
                     /translation="MKKFYPDIRSAQIKVGLFTIIISILLVLGYLWLTNRVSTRSLQD
                     LSVSFEDIAGLEVGDKVMYRGMEVGRIRQVEQRDDHVIVKARIHKDIRLREGSSFQIG
                     DSSLMGGTALSINPGPGPGWLSLSKVLPGDAPSGIMSIVAKASATMQELNLVMAELRK
                     ERGVIDKSASLLDDAGTAVRNVDNLASGLGKDFSLTLKRLDTLTAQIDALVDANADSL
                     NKSIGSAPSAIAGLHGTLDSLRVLSGQLNKAAGKLNSGTGTAGRLMSDEELYTRLLES
                     VNNLDALVKDVKAHPKKYVKFSLF"
     gene            complement(8694..9872)
                     /locus_tag="PLO35_05175"
     CDS             complement(8694..9872)
                     /locus_tag="PLO35_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOH79092.1"
                     /translation="MDISQTNHLKAYGVAFAALQKQHVVKWLLNYRGGSFLMPSDPAM
                     EALCNIRGVRYETIGGAEVAAIMAEIQDANMEAVVLEKEPKIAIYIPPNTLPWDDAVT
                     LALEYAEIGYDRFWDEEVLEGKLRDYDWLHLHHEDFSGQYGKFYGSYRHTPWYQNDVR
                     VNEAMAARHGYSKVWQLKHAVADRIRDFVFEGGFMFAMCAATDTYDIAMAAHGIDIVD
                     AAIDGDGIDPDFQNKLDYSRCFAFEGFKLRTSPYEYEFSDLDASDYSRLRGAEADYFQ
                     LFDFSAKYDPVPTMLTQCHVNVVDGFLGQTTSFFKDKVKKSVIILGEVPGANEVKYIH
                     GNIGKGTFTFYGGHDPEDYQHKVGDPETILDLFKNSPGYRLILNNILFPAAQKKELKT
                     "
     gene            complement(9947..11332)
                     /locus_tag="PLO35_05180"
     CDS             complement(9947..11332)
                     /locus_tag="PLO35_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOH79093.1"
                     /translation="MKLEELRLRIRAAIGDSVLLTKSYFAGGSVRDMLLGLTDNDPQD
                     VDIAVEAENGGEELARLLHERLGGSAPVVYNSFGTASIVLEGLRLEFVHTRRESYRSL
                     NRKPSVSLGTLSDDALRRDFGINALYMSINDGGILDPSARGLKDLQDRVIRCVDHPER
                     VFREDPLRLLRALRFSVRFGFEIEAATWEGILNNAGWVKHLSWERIASEVNAMLTVPD
                     PDKSVRAIRLLQESGLLAYILPELNDLRGLTQNRYHHLDAFEHSLEVLRHSAADPVAR
                     WAALLHDIGKASTLKLKADGTHSFIGHETVSATRALGILRRFKLPKQQRDLVSQLIGG
                     HMLFKQSGPEGMAMKDSSLLRIADRFQDALWQLLDLAEADNLAHAREHRNPDQIPGLR
                     RRFAAFKTSLPKFSLTGRDLIQEFGISQGIRIGKLLNLAKQAWYEDPGLGREQLLELT
                     RRHLEQQEETN"
     gene            complement(11329..12789)
                     /locus_tag="PLO35_05185"
     CDS             complement(11329..12789)
                     /locus_tag="PLO35_05185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HOH79094.1"
                     /translation="MGQNLPAGPLAVYLHFPFCLKPCPYCSFFKRPWSRSAHERYLQA
                     LLQEAELWEKRFGRRVPASSLYFGGGTPSLLKGEEILRLCECFDLKQRDRGTEAFYSM
                     RNERTRGNDKQRWSSSGHDKGMYDSEFAGRLDFNQHARNPDCSDGHAGKHDYSEVLLE
                     VQSPFRRRDKRITCLGALSSKVADCDPVTRNALRITSFPEITLEVNPIQITPDFVKGL
                     RTTPVNRLSIGVQSMRDEALGYLDRAHKAASIPERIRLLRDQGYDNISLDLIYGLPGM
                     DLDDLKRDLDAFVALGPEHISCYLLTLDEDSELARAIAEGKSPSLPDDESCALQYELI
                     CQVLKKAGFIHYEISNFARPGFEGRHNLSYWHSSPYLALGTAASGWLPPKRYCNPASL
                     DEYYRLIEAGELMPGAETLSPAQSEADEIMMGLRLLEGIDLKNFETRHLHPFERGRER
                     ELERLIELELLERGDGRIRLSERALFISNAVIGELL"
     gene            complement(12759..13703)
                     /gene="lepB"
                     /locus_tag="PLO35_05190"
     CDS             complement(12759..13703)
                     /gene="lepB"
                     /locus_tag="PLO35_05190"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH79095.1"
                     /translation="MKDRPIAKNLRQKIDSKELAYTITPLPEGTKIKRRKPWLQDWIE
                     AILFAFVVAMIIRNYTFQNFVIPSSSMEKTLLVGDYLVANKLKYFVKDPQREDIVTFR
                     YPKIEEGTPEHPKSKDDFVRLFHPLYLNKKYAFGKFPLTFFHLTYYARKNVVKRVIGM
                     PGDIVEVRDKIVYLNGKRFERGYECYDVADPAPPLAPHRVFQHRLAPGNYDYVSQNPW
                     YQPYIVKPAGADSSAVLVSNGIFNRDWFGPIKVPEGKYFVLGDNRDVSEDSRYWGFLD
                     RSDITGTPWLIFFSKGIEYNKINDVPHTRWDRIFRLAR"
BASE COUNT         2974 a         3757 c         3845 g         3188 t
ORIGIN      
        1 ttggagcgga gatgtgagag ctacgctggt gagagccgtt ccggctggtt agagctccgc
       61 gagctagtga gggccgcgac tgacaatgcg aagcaggata tacagctgtg cccacagcag
      121 gataaggcta atcccaaaag caaggatgcg attgggccgc ggattgaaaa agagctgaac
      181 tatgacggga tagccgtccg acctttgcca gcgatccaga tatccgtcca attctggatc
      241 gggacggatc tgaatcagtt ccatcagctg tttgatatgc ttttgatgat aggcccggcc
      301 ggccagttcg ccctcggcgc tgtagaggct aaatcctttc ttatcaaagc gtctgatcgc
      361 ttcccggaga ccccggaaac cccggtcaaa caacttctcg agaccgggat cggatgtgag
      421 ctcccaggtc ctgtgcagtt ctaaaaggac cgcggcgtga ccgttgagca catagggggc
      481 cttgtctccg ggatattcca tgaaccagag gccgtcccta cgcttgtggc caggatcttc
      541 catagtgttg atggacatgg tgatagtgag ttcggaggga ggtttgaggc tctgggccag
      601 agcttgggaa cgatggagcc agacgcgctc ccgggtcagt ttccagcgcc gcgcgcaaac
      661 cgtcatcgct tccgcctgag ccaaggcgct gtgccaggga gctttcatct caaatttggg
      721 ataatcgaaa tcgtaagaga gccagagagc tttgcccttc tggctcatag cttttcccag
      781 ccagtcactt agcttgataa aacgggccct ggccagtgaa tccggcagct cttcatacaa
      841 ctgattggcc acagaggcca cgaagagggg attgtagtgc tgggagccgt ccgcgtaggt
      901 ctgatatgga atgccgtcgc tatccagacg ctggacctgg tttactggcc gtccacggac
      961 aagctggctg agttcctctc ccagcttgag cagacgcgtc tcggcatact gccagatgaa
     1021 agcgtgcagg acgatggcga taaccaacgc caaagaccag ggtctcagaa tcgtctcctg
     1081 atcacgaggc tgaggatgaa gagcacaaac tccagcatca cgatattggc tccggtagga
     1141 aggtccagcc accaggacag cgcgaagcct cccagcgcgg ctatcactgc caccaacgca
     1201 ctgaggacga tcacgatcgt gagccgccgg gccagattga aagcagttgc cgcgggcaga
     1261 atgagttgcg cgctcaccag gatgatcccg gccgctttga gattgatcac gatgttggca
     1321 gccagcaaag ccagaaagag ccggtttacc aaaaccaccg gcacccgata gaatcccgcc
     1381 atttcagagt tgtaagatag atagaaaagt tccttgtaga gcagcaggat aaatcccagg
     1441 ctcagcagcg ccaggatgcc cacccggatc aggtctgcct ggctgacaag cagcacattg
     1501 ccaaacagat agctggccag atcgaagctg tagctctttt tcagattgat cagaatgata
     1561 cccagggcca tcgagacgga caaaaagata gtgatcgtgt tggcttcttc aagcttatgc
     1621 ttttgggaaa gccagccgat ggccagagct accagcacca cgaatgccaa agcggtgaat
     1681 tgaatatcca ggcccaccag caacgcgatc gcgatcccgg cgaaggccat atgtgacaag
     1741 gcttcaccca gataggagat tcgccgcaga gtcacaaaag gcgaaaacat ggccagagaa
     1801 accgaagaca gcagagctgc cagaagcgcg cggaccagaa agctgtagct aagcatcgag
     1861 aatggtgaat tgagagtgga aaattgagaa tggaggatta gcaggggttt ctgagaaaat
     1921 ctcagttcca tgtcctgatt ggtccaggtg gagcagtttc atctgtgtca tccgcttgct
     1981 attcattagt ttaaagcaga ggacaagagg tggcatgcag gcccgaatgc cgatttggca
     2041 cctgatctcc agccaagcct ggtcgctccg gttctggttc tgcgggcaag ctgtaaaccc
     2101 tttatgctct ctctctctgt gcctctgttt taaaagaacc cgcatctcag tacctcttct
     2161 cgatgatgcg caccgcgtct ccaaaagtct tatggatgag ctcggcgttg atcatttcgg
     2221 catgatcgtg gcagtggaga cggcggttga ggcagaccag gaagctgcag taatcggtga
     2281 gggtgtggag atcgtgggag attgtgatga tggtgcggcc ctgcttgttg agctctttga
     2341 ggagctcaaa aaagctgcgc acgccaggct ggtcaaccga ggcttccggt tcatccagaa
     2401 tgaggtattt gctgccgccc acgatggccc tggccagcag gacccgctgc atttctccac
     2461 cggaaaggcc tccgatcggt ttgcgggctt gttcctggca cccggtagtc cgcatggctt
     2521 cctgggcccg atcgcgttgt tgacggctaa agcgctgccc cagtttcagt tctcctgcca
     2581 ggcccagcat cacgatatcg agcgcccggg ctgggaagcg gcggtcatac tcctcccttt
     2641 gaggaagata gcccatggca tgggttttca gccattgcag atgaggctcg ccgtctatgc
     2701 tgatgctacc ttgctggagc gggatgaggc ccaaaatcag tttaatgagg gtggatttac
     2761 cggccccatt gggaccaata atcgctacaa actcgccttc ggcgatctcg agctggatat
     2821 cttccaggat gggaatcccc gcgatggcat gattgagatc tttgatccgg atcatggttt
     2881 gacggagggt ttctcctgat tccagctcaa tttcatgcgt tcccagttac tccagatcag
     2941 gtcgataagg cgagccgagc catggctttg gcccagaggg tccagttcca gtaaagccag
     3001 gccaaattca tcggccagca ccttggcgga gcgtttgtcc atctgaggct caacgaagat
     3061 ggccttgatc ttgttggttt tgatcactgc ccccaggaga gtcagttccc tggcagtagg
     3121 ttcctgtccg ggagagcttt gcaccgtgcc catgatctgg acgtcgtagt ctttggcaaa
     3181 ccagtgaaag ctgtcgtgat aggtgatcag gatcgggacc gcgatcgcgg cacgctcgct
     3241 ggtgatctga cgatgcacgg cggccagatc gttgatcatg cgggaggtgt tggccctgat
     3301 ggtggctgcc tgggacggga agcgggtctg aagctcgtcg ctgagcatga ttaccgccct
     3361 cagcatgagc tggggagaaa gccagatgtg gggattcatc ccggcatggg catggtctgt
     3421 ttccactgcc agggtgctgt cggcctgaac ctgagcctga tcttccagct tgagcagttt
     3481 ggaaacatcg atcagtttgg cggagcgctc ctcgaaggct ccggtaagtt cggtttcaag
     3541 tcccagtcca ttgatcacca gcagtttggc atcatccagg gacttcatat ccgccggttt
     3601 ggccgtccag gtgtgaggtg aggctttggg tggaatcagg ctgcgcacct gaatgctgtc
     3661 acccacgatc tcctggatca ggagtttgta gggatgtata gaggtcacga tgaggggacg
     3721 gtcgtccttg acgcgaggtc tgcaccccag caaggccagg cccgcgatca aagtgaccat
     3781 gatgatcacc cttatccatg cggagcgctg cgagcagttg tttttcataa attgttacct
     3841 tcgtttacca gagcataagc tgggagttga ttgaatcttc gggattgatc gcggggccag
     3901 accgcaatcc agccccgcta tcggattatt ccatctctct gaggagttga gtgatcagac
     3961 gcacacccac tccggtagcg ccgacgctat acatgccgga ttcaccgtcg cggagggctg
     4021 tgccggctat atctatatgc agccagggtt tgtcttccac aaattcacgg atgaagagag
     4081 ccgctgtgat ggtgccggca aagggaccac cggtgttttt gagatcggcg atctggctct
     4141 tgatgagctc tttatagtct tcgtgggcgg gcagttgcca gatcttttcg ccgctaaagt
     4201 cggaagccgc tttcagcttg tccagccagg catcgttgtt ggtgaccacg gctgagatct
     4261 gattccccaa agcacccacc gcggctccgg taagggtagc gatatccaac accttgtcca
     4321 cgtgttcttt gctaatggca aagtggatgg cgtccaccaa agtgagacga ccttcagcat
     4381 cggtattgat cacttcgatg gtcttgccgg ccatagagcg gatcaggtct ccggtgtgat
     4441 aagcgtcacc cgagatcatg ttttcacagg cggccacgat gcccaccaga tttaccttga
     4501 gcttgagagt ggcggcagcg ctcatggcac ctatcaccgc tgcggctcca gccatgtcgt
     4561 ttttcatgtt gaccatgccc tggggggtct tgatgcagta tccaccgctg tcataggtga
     4621 gacctttccc gatcaggccg atcgtcttgt ttttggcgtc gggattgccg ttgtagcgca
     4681 tgatgatgag ccgcggctcg gctttggagc ctttgcccac cgccagaaag gcgtccatct
     4741 tgatgcggcg gatcttgtcc gcggtgtaga tctcgatatt gaagccatat tggagggcgg
     4801 cttttttggc tgcttcagcc agggtttccg gatagatcac gttggccggt tcgttgacga
     4861 gctgacgcgc catattggtg gtctcggcaa agatgcgtcc ttccaggatg ccgcggttga
     4921 tatggcgggt gtttttgagg ttgactgcca ggtgcagcgc ttcgatggca tgctgtttgt
     4981 ccgtgctcag atacttggtg aatttatact ctgtcagcag ggcagcttca gccaggacgt
     5041 gaccaaactg cacatccccg atcggaatct ggaaaccggg aaacaggtat atcagcttgg
     5101 cattgaaccg gatagcggca cgggtgatat cggcaaacag ctttctcagc ctgtccggtg
     5161 tgagggcggc aggatcacct gccccgcaga tcacgacgtt ggcccgatgg ccatcctgga
     5221 ctgtctggaa gctcttcagg gccgcgtaat ccggattgta gccctcgctt ttgatatagg
     5281 cggttacgag ttccttgtag ctttcgggca ggtactcgat agactcgatt ttgcttttgt
     5341 cgtccagcag cagtaccacg gtgtccatgt gctccggaag cttgcgcaag agatcgatct
     5401 tcatgtgggg ttctccttgt atgtttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnccga tgccaaaatc ggagcgcttt ttcagataga gatagctgag caggatatcg
     5581 ttttcttcca ggatgccgga aaatccggca ttgatgccaa taccgtggtt gcccatgata
     5641 tctgccagcc ccagttccac atagccaagc gcccccaccg aagaggaata ggccagcccg
     5701 ccccagaggt gttcgatggc aaaaccgctg cggtatttct ccgtcctggg tagtttgggt
     5761 cccggctggg tggggcggtc atcgtagctg aaatcctgga aaaacagcag gctgtcctgg
     5821 cgggtgaatt caaacttggg ttcttcgccg atcaaaggac ggcgggcgtc gtattgggcg
     5881 gcgggattga tccgtttggg cctggcttta gggcggcggc cgaagaggtc cagttggctc
     5941 aggtccacgc cgtcccacat gtcatccttg ccactatagg gttgcggggc cataaccggt
     6001 tggtattcaa ggctatccag ggggttgtag ctgaagtaga tgtcccaggc gccgttgaaa
     6061 tagttcgaga ccagcagata gcgtccgtcc cgggaaagat ctccgctata ggcccctgcc
     6121 aggacgttgg tcacagcggc tttggttcct gcttccagat ccaggacgcg gaaattggcc
     6181 actccctgat cactggagat atagagtagt ttcttgccgc cggcgtccaa catcggatag
     6241 ctacagttgc ctggctcgtg actcatctgg gtgagctctc cggaagccag gtgcagctga
     6301 tagatcgccg agcggtaatt ggcaaagaaa cctctgcgct tgccttcggg attcaggatc
     6361 cgctccgacg cgaagacgat ggactgtcca tcgggagccc agcgggcctg agcgtcccag
     6421 taaagatcgt ctgtgaggcg tccaagtgtt ttgctggcaa tatcatatac aaagatatcg
     6481 ctctgcatat ccttttgccc cgagatcaat atcctggtcc catccggcga aacatccagc
     6541 tcgtagatgc ctctcaactc gggaatactg atgctggaga cgattttttc ggtgtgggta
     6601 tccagaatgt ggatcacatc gccaactgag tttttggccc cgaaagccac tctgcggcta
     6661 tccggaaacc agctcaggtt ggcgcggaaa tagtagaatt cctccatctt gccggtcgct
     6721 tcaccggtga tcagcttccg gggcctggaa agcccatggg tgccagacat ccaaacgctg
     6781 taacgggctc cctggttgga gaaatacaca tagttctgtc catccggaga aaaacggggc
     6841 atgtagttga aataggagcc atcgcgagcg ctgaaagtac gctgctccat gtcttccttg
     6901 ggagtgccgt gctggttcac ggccgggaaa tacaagcgtt tgagatgata gcgccagcgg
     6961 gattccaact cctcaagctc catgccaaag accttcttgg tagcctcatc ggtaccgcgg
     7021 gaactcctga gcgcgaagta gtagtcgctc accttttctc tgccccagac ctccgcgata
     7081 taggacagga agctttctcc cagccgatag gccagatagc cgtccgaata ctccagagga
     7141 ggcagactct cgttgaccac catatcgagg atgaacatat tgttgtagtc atcttccccg
     7201 ccgatcgata gaaactccgg caggccttcc gaaaaccaga agggaaatga ggctggtctg
     7261 agcgagctca ccaggctgga gccgcgctcg atggcgttgg tatatgcgtg ggtaagctcg
     7321 tgggccataa gctcttccag cttggcatag ctgccctcga agggaatcac cacccggttg
     7381 cgaaggcttt ccgtgaagcc accaatccct tccgtgagca gcggatagat gatattggtg
     7441 ccctgaaact ccgtcttggt gccatagatg atgagcggga ccctggaaga cagtggaaaa
     7501 cgcaggtctt gcttgagcgc gtaataggtc tcctcagcca tgagggtggc caggcggcca
     7561 aagtcatcgt tcccggcagg aaaatagacg tcgaagtgca tgctgcggat catgctccag
     7621 ttgctgtcca cggcattgac tttgttctgg ccaaagctgt aggcgttaag tgtaacaaag
     7681 ctgctcagga gcagcagggc caggatggca atcacggtct tctccactcc tctgtccctc
     7741 tgtccctctg ttttataatg actcatctaa ccaaccctat cgtctaaaac agactgaact
     7801 tcacgtattt cttggggtgg gccttgacgt ctttcaccag cgcgtcgagg ttgttcaccg
     7861 attccagcag gcgggtgtag agctcttcat cgctcatcag gcgcccggcc gtaccggtgc
     7921 cggaattgag ttttccagcc gctttgttga gctgaccgct cagcactctc aggctgtcca
     7981 gggtaccgtg cagtcccgcg atggctgaag gcgcggatcc gatggattta ttgaggctgt
     8041 cggcattggc atccacgagg gcatcgatct gggccgtgag ggtatccaaa cgcttgagcg
     8101 tgaggctgaa atccttgccc agaccactgg cgaggttatc cacattgcgg accgccgtgc
     8161 cagcatcatc cagcagactg gcacttttgt cgatcacgcc tcgctccttg cgcagttccg
     8221 ccatcaccag attgagctcc tgcatagtag cgctggcttt ggccacgatg ctcatgatcc
     8281 cgctgggagc gtctccgggc aacactttac tgagggaaag ccagccgggc ccgggaccgg
     8341 gattgatgct gagcgccgtt ccacccatga gagacgaatc tccgatctga aagctgctgc
     8401 cctcccgtag acggatgtct ttgtggatgc gggctttgac gatcacgtgg tcgtcccgtt
     8461 gctccacctg gcggatcctg cccacttcca tgccgcggta catcactttg tcgccaacct
     8521 ccaggccggc aatatcttca aagctgacgc tgaggtcttg cagggagcgg gtggaaaccc
     8581 ggtttgtgag ccataaatag cccaatacca gaaggatgga gatgatgatg gtaaacagcc
     8641 ccaccttgat ctgagcgctg cggatatcgg gatagaattt tttcatgagg ccttcaggtc
     8701 tttaattcct tcttctgcgc ggcaggaaag aggatgttgt tgaggatcaa ccggtagccc
     8761 ggagagtttt tgaacaggtc aagaatcgtc tcgggatcac ccactttgtg ctgataatcc
     8821 tcgggatcgt gaccaccgta aaaggtgaag gtgcccttgc cgatattgcc atggatatat
     8881 ttaacttcat tggcaccagg cacttcgcca aggataatga cgctcttttt gaccttgtcc
     8941 ttaaagaaag aggtagtctg gcccaaaaag ccatccacca cgttcacgtg gcattgagtg
     9001 agcatggtgg gcacgggatc gtatttggcg ctaaagtcaa agagctgaaa gtaatccgcc
     9061 tccgcgcccc tgagcctgga atagtcactg gcgtccaaat cggaaaactc atattcataa
     9121 ggactggtgc gcagtttgaa gccctcaaag gcaaagcagc ggctgtagtc cagcttgttc
     9181 tgaaagtccg gatcgatacc gtctccgtcg atagccgcgt ccactatatc gatcccgtgg
     9241 gccgccatgg caatgtcata ggtgtcggtg gccgcgcaca tggcaaacat aaatccgccc
     9301 tcaaacacaa aatcccggat gcgatccgcc acggcatgct tgagctgcca gaccttggaa
     9361 tagccgtgcc gggcagccat agcttcattg acccgcacgt cgttttgata ccagggagtg
     9421 tgacgatagc tgccgtaaaa cttgccatac tgaccgctga agtcctcatg gtgcagatgc
     9481 agccagtcat agtccctgag cttgccctcc agcacctcct catcccagaa gcgatcatac
     9541 ccgatctcgg catactcaag tgccagagtg acggcgtcgt cccagggcaa agtattggga
     9601 ggaatataga tggcaatctt gggctctttc tccagcacca ccgcttccat attggcgtcc
     9661 tggatttcgg ccatgatggc agccacttcc gcgccgccaa tcgtctcata gcggactcca
     9721 cggatgttgc aaagcgcttc catggctgga tcggatggca tcagaaagct accgccgcga
     9781 tagttgagca gccattttac cacgtgctgc ttctgcagcg ccgcgaaggc aactccatag
     9841 gccttgaggt ggttggtctg gctgatatcc atcggcacca gtagctccgc ggtcagcatg
     9901 ttgaccgtca agatcagcgc caggagcatc agccccgtct ttggcttcaa ttggtctcct
     9961 cttgttgttc cagatgccgg cgcgtcagct ccagcagctg ttctcttccc aggccgggat
    10021 cctcatacca agcctgtttg gccaggttga gcagttttcc tattctaata ccttgggaaa
    10081 tgccaaattc ctgtatcaag tctctgcctg tcaagctaaa ttttggcaag gatgttttga
    10141 acgcggcaaa acgacgtctc agaccgggaa tctgatctgg attgcgatgc tccctggcgt
    10201 gggccaggtt gtctgcctcg gccaagtcca atagctgcca gagcgcgtcc tgaaagcgat
    10261 cggcaatcct cagcaagctg ctgtccttca tggccatacc ttccggtccg ctctgtttga
    10321 agagcatgtg cccgccgatc aactggctaa ccagatcccg ctgctgtttg ggcagcttga
    10381 agcgtctcaa aatccccaaa gccctcgtcg cgctcaccgt ctcatgaccg ataaaactat
    10441 gggtgccatc ggctttgagc ttcagggtgg aggccttgcc gatatcgtga agcagggccg
    10501 cccatctggc tacgggatcg gcagcgctgt gtcgcagcac ctccagactg tgctcgaagg
    10561 catccaggtg atggtagcgg ttctgggtaa gtccccgtaa atcatttagt tccggcagga
    10621 tataggccag cagaccgctt tcctgcaaga gccggatagc acgcaccgac ttatccggat
    10681 cgggaaccgt cagcatggcg tttacttcag acgctatcct ctcccaggag agatgcttta
    10741 cccagccggc gttgttcaaa atcccttccc aggtggcggc ttcaatttcg aaaccaaaac
    10801 gtacgctgaa gcgaagggcc ctcagcagcc tcagagggtc ctcccgaaac actctttcag
    10861 gatggtccac acagcggatc accctgtctt gcaaatcttt gagcccgcgc gcgctggggt
    10921 ccagtatccc cccatcattg atactcatgt agagcgcgtt gatcccaaaa tcccgcctga
    10981 gcgcgtcgtc tgatagtgtt cccaggctca cactgggctt gcggttaagg gaccggtagg
    11041 attccctgcg ggtgtgcaca aactccagac gcaggccttc caaaacgatg gaggcggtac
    11101 caaagctgtt atagaccacc ggagcgctgc cgcccaggcg ctcatgcagc agcctggcca
    11161 gctcttcccc accgttttcg gcttcgacgg caatatcgac gtcctgaggg tcattatcag
    11221 tcaggcccag gagcatatct ctcacactgc cccccgcgaa gtagctttta gtcagcagca
    11281 cggaatcgcc aatcgccgcg cggatcctca gcctcagctc ttccagcttc ataagagctc
    11341 accgatcacg gcattggaga taaacaaggc acgttcagag agcctgatcc gtccatcgcc
    11401 acgctccagg agttccagct ctatcagccg ttccagttcc cgttcccttc cccgttcaaa
    11461 tgggtgtaag tgtctggtct caaagttttt caggtctatc ccctccagca gcctgaggcc
    11521 catcatgatc tcatccgctt cactctgcgc ggggctcagg gtctcggctc cgggcatcag
    11581 ttctcccgct tctatcagac ggtaatactc atccagggaa gcgggattgc agtagcgttt
    11641 gggaggcagc cagccgcttg ccgcagtccc cagggccaga taggggctac tgtgccaata
    11701 gcttagattg tgccggccct caaatccagg ccgggcaaaa ttggagatct catagtggat
    11761 gaatcctgcc tttttaagga cctggcagat cagctcatac tgcagcgcgc agctttcgtc
    11821 gtcagggagg ctgggacttt taccttccgc tatcgctctg gccaattcgc tatcctcatc
    11881 cagggtgagc agatagcagg atatatgctc cggacccagg gccacaaagg catccaggtc
    11941 ccgcttcaga tcatccaggt ccattccggg caggccgtag atgaggtcca gactgatatt
    12001 gtcataaccc tgatctctga gcagcctgat tctctccgga attgaggccg ctttgtgagc
    12061 gcggtccaaa tagccgaggg cctcgtcccg catgctctga acgccgatgg aaagccggtt
    12121 tacgggtgtc gtgcgcaatc ccttgacaaa atctggggtg atctggatgg gattgacctc
    12181 gagagtgatt tctgggaagg acgtgatgcg taacgcgtta cgtgtcacgg gatcgcagtc
    12241 agccaccttg gagctcaaag cccctaaaca agttatgcgt ttatctctac gcctgaaagg
    12301 gctttggact tcaagcagta cttcggagta atcatgttta ccagcgtgcc catcggaaca
    12361 atcaggatta cgagcgtgct ggttgaagtc caagcggccc gcaaactcgc tgtcatacat
    12421 acctttatca tggccgcttg aactccaacg ttgcttgtca ttccctctcg tcctctcatt
    12481 tctcattgaa taaaaagcct ctgtccctct gtccctctgt tttaaatcaa aacactcgca
    12541 cagccgcagg atttcctccc ctttcagcag cgatggagtc ccccctccaa agtagaggct
    12601 ggaggctgga acgcgccttc caaagcgctt ttcccacagc tccgcttctt gcaacagcgc
    12661 ttgcagatag cgttcatggg cgcttctgga ccagggcctc ttgaagaatg aacaatatgg
    12721 acagggtttg aggcagaaag gaaagtggag ataaaccgct aacgggccag ccggaagatt
    12781 ctgtcccatc gagtgtgggg aacgtcgttt atcttgttgt attctatgcc tttggaaaag
    12841 aagatcagcc aaggagtacc agtgatatca ctcctgtcca gaaagcccca gtagcggctg
    12901 tcttcactca cgtcgcgatt gtcacccagc acaaagtatt tgccctcagg cactttgatg
    12961 gggccaaacc aatcccggtt gaagatcccg ttgctcacca acacggcaga gctgtccgct
    13021 ccggctggct tgacgatata gggctgatac caggggtttt ggctcacata gtcatagttg
    13081 ccgggggcca gacgatgctg gaagacccga tgcggtgcca ggggtggagc tggatcggcc
    13141 acatcgtagc attcatagcc gcgctcaaag cgtttcccgt tgagatacac gatcttatcc
    13201 cgcacttcca caatgtcgcc aggcatgccg attacccgct tcaccacgtt ttttctggca
    13261 tagtaagtga ggtgaaagaa ggtgagcgga aacttgccaa aggcgtactt tttattcaga
    13321 tagagcggat gaaaaaggcg cacaaaatcg tctttggatt tggggtgctc aggggtgcct
    13381 tcttcgatct tgggatagcg gaaggtgacg atatcctcgc gctgcggatc tttgacgaaa
    13441 tacttcagct tgttggccac cagataatcc cccaccaaca gagtcttttc catcgaggag
    13501 gaggggatca cgaaattctg gaaggtgtag ttgcggatga tcatcgccac cacaaaggcg
    13561 aagaggatgg cttcaatcca atcctggagc cagggcttgc gacgcttgat tttagtaccc
    13621 tccggtaaag gagtgatcgt gtaggcaagc tctttgctat ctatcttttg gcgaaggttt
    13681 ttggcgatgg gtctgtcttt catggttgct tttcctgccg attttagcat tttttggtac
    13741 aatcctgata gggctctcat ttgtcaagcc ggaaaagcca aagctcattt cagcggcttt
    13801 cgcaacaccc taccccggct ccactctgag tattgccaac aacaagacca tttctatttg
    13861 ggcg
//
LOCUS       DAOSFN010000059        13654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3736_length_13654_cov_8.022575, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000059 DAOSFN010000000
VERSION     DAOSFN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13654
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3736_length_13654_cov_8.022575"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..1893
                     /locus_tag="PLO35_05195"
     CDS             295..1893
                     /locus_tag="PLO35_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH79096.1"
                     /translation="MKNICVIGLLVIALLAAGCSTWRGKKQRPELDFYVGGETATAYE
                     NYIPIVAMEAPMPEMNTDEFAAIEPNRFQRADLQPLSTFSIDVDTGGYSIVRKFLNDN
                     ELPPANAIRSEELINYFSYDYPKPDGKHPFSVYTELASCPWNDQHQLVHIGLKGMELE
                     TKDLPSSNLVFLIDVSGSMDEPNKLPLVQSSLSMLTDQLRENDYVSIVVYAGAAGLVL
                     PPTSGMHKDKIKKALYDLEAGGSTAGGEGIQLAYKTAADNLIKEGNNRVILCSDGDFN
                     VGISSTAELVDYAKKQRDSGIFISILGYGMGNYKDNRMEQIANQGNGNYAYIDDILEA
                     KKVLVNEMSGTLLTIAKDVKIQVEFNPAVVGAYRLIGYENRMLQAEDFKDDTKDAGEM
                     GAGHSVTALYEIITLESGENLPDIDSLRYAKPAVPADASTAEMLFVKLRYKLPDGDES
                     IPLDQPLPNKSLPWQESSQNFRWAAAVAVYSMLLQENPNQGMADWDLVMSLAKSSKGE
                     DPNGYRAEFIRLAEKASLMKPIPK"
     gene            2241..2741
                     /locus_tag="PLO35_05200"
     CDS             2241..2741
                     /locus_tag="PLO35_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOH79097.1"
                     /translation="MSNQIKKWIATILGLHMLFIAPVIAESVAGLGSDEIIQDHSALV
                     IDSLHTQEPPGNPPGERMVASYYSGKFHGRKTASGERFDQYALTCAHKTLPFQTILKV
                     TNPKNGKSVTVRVNDRGPFTRGRDVDLSYAAAKEIDMIQAGVLAVEVLVLPPSETTED
                     AIASSR"
     gene            2825..3766
                     /locus_tag="PLO35_05205"
     CDS             2825..3766
                     /locus_tag="PLO35_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOH79098.1"
                     /translation="MKTHPGIPLSCSEAVSQALESNQPIVALESTVITHGLPYPQNFS
                     LLQRMEAIITDLGAVPATIIVWEGKAHIGIGPKLLDEIKATLSLTQSAFMKLGSRELP
                     LAFARGVNGGTTVSATMLLAQLAGIEVFATGGIGGVHRGWQQSMDISMDLEALSSIPV
                     MVVSAGCKAILDVPATLEKLETLAVPILGWQTGSFPLFYTATSPYPIDRCDSIDQIVR
                     AWRFHKALQTSSSGMLIANPIPAEHSIPSSDIDPYIDTAIEAAAQAGAHGKALTPFLL
                     DYLARATEGASIRANLALLENNARLAARIARALKDDI"
     gene            3763..4605
                     /locus_tag="PLO35_05210"
     CDS             3763..4605
                     /locus_tag="PLO35_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOH79099.1"
                     /translation="MKLYLSLDMEGMPGTFSWEHEKTDRSAVKRYFRAHVQNVLETIL
                     SHPASASLEEIRIADSHSAGDNLDYDITALDPRISLISGSPRPLYMMPALERGFDQVW
                     LLGYHAGTGALRGNMDHTYSNSRIHKIFINGQAMNEALINAAYAGYMGVPVALVSGDT
                     TLQAELHSKAALPWIQYVPTKTAVAKFAARSFSSLKVAEYVQTAVNQALVTPKAELPL
                     YRFDAPITLKIEFHSTAMTDQACLMPHVKRLDGRVIEYSDPDYGVIFEAIMALVTLAS
                     TANM"
     gene            4656..5447
                     /gene="pyrE"
                     /locus_tag="PLO35_05215"
     CDS             4656..5447
                     /gene="pyrE"
                     /locus_tag="PLO35_05215"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOH79100.1"
                     /translation="MDNYKTIVRFEDIFEAELAKSLLIDNGIDASLLNERMMSMAPGL
                     ASSKLNLELQVPESQEAAALEILAGSVSENETENMLKSEGAILEGHFQLTSGRHSNLY
                     VEKIRLLQNPAVAKLLCSKLAELISEHEFDCVVGPAYGGIALAFEVASLLDKSFVFCQ
                     RQDGMMSIRSGFDLSGVKHAAVIEDIVTTGGSVKEVIACLNARGVEVSVVAALVDRGS
                     GKVDFGLPFVSLLQLDIPSWEPSDCPLCAEGIPIRKPGSSDKVSI"
     gene            5459..6886
                     /locus_tag="PLO35_05220"
     CDS             5459..6886
                     /locus_tag="PLO35_05220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH79101.1"
                     /translation="MPRIDLTASTASEAKLLAELLSFFDPPEGVRLRFLCPPEARKDI
                     ELKHKWLEIIIDKPKPEHQVQVVCSSSAGNLARESLQLWSSNALSCIELGRDPSLFKQ
                     AREFCVGYRFYCLDSERLNLKGIEPLQVWPELSTGIASQAKTDILVTRSPELRQALFI
                     DSPEDLWHILITAEGNLLDLRLDDSAEAYLRAYRQLMALCQALSESQQISLEALRRNY
                     DKIHGSYSFFAEFYDRYMAHVDYSAWLTMILSWARRFYAGPLVRALELACGTANIAEQ
                     LVFKGMEVDACDLSPFMLREAARKCFKPHLFRASLDTPLPHRDCYDLVLCLFDSVNYL
                     LDIAELKATLTHVRAALKPGALFVFDISTLQNSRENFDDLVQHHRFGNALIVHHASFD
                     ELGMRQKSSLTLYRKRHLGFVMSEEHHTQRVYRHREILSVIASSQMELLGIFNPDTRS
                     NLLSKRHQNLDEQYSRLFYMLRRPL"
     gene            6883..7530
                     /locus_tag="PLO35_05225"
     CDS             6883..7530
                     /locus_tag="PLO35_05225"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOH79102.1"
                     /translation="MSELYNRDLALLTDHPLLMGVDEAGRGALAGPVVCAAVILNYGE
                     QIEGLNDSKLVSAKRRDQLYDKILANAMAFKIINISPAYIDAHNILQATMEGMRQAID
                     GIACGQCLCLIDGNQLPFKHCQSRHPQLVQQTVVDGDALHACIAAASILAKVTRDRLM
                     IELHPQNPFYGFDRHKGYGTTAHLKALRQHGPCEAHRMSFGPIREVLQIMDIFHP"
     gene            7970..8767
                     /locus_tag="PLO35_05230"
     CDS             7970..8767
                     /locus_tag="PLO35_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOH79103.1"
                     /translation="MRYRVAVLQYEPRLLEPQANLEKLLDMLRGLETDLVVLPELALS
                     GYVFASREEVAKVSESIPDGACFQALRDISITRDFSLVYGFAEHTNGCFYNSSALINP
                     DGSWHVYRKTHLFNREKLFFTPGDTGFEVHKAKHEVPIGMMICFDWQFPEAARTLSLK
                     GARIICHPSNLVLPWCQQAMITRSLENRVFSITANRVGTERNGDLEETFTGASQILST
                     KGEILVRMETTGSGVSTCEINPDLAASKKVTDRNDAFADRRPEFYQL"
     gene            8764..10755
                     /gene="ligA"
                     /locus_tag="PLO35_05235"
     CDS             8764..10755
                     /gene="ligA"
                     /locus_tag="PLO35_05235"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOH79104.1"
                     /translation="MNKDALISEISRLKAEIERHDVLYYQFANPSISDYEYDQMARRL
                     KALEAELNETPPEDSPTQRVGNDLKQGSGTIPHKQRMYSLDNAYSIEEARDWYHKLSQ
                     ELGAPPVCDLELKIDGFSINLYYQNGQLQYASTRGDGITGEDVTVNVKTIESIPDRIE
                     FQGSIEIRGEIYMPVAEFIALNEARAANEEKTFANPRNAAAGSIKLKDSQAVRKRKLQ
                     ALFYSYGYADPLPASTQSGLLEWLHGQGFPVSDASRTAVSFSEIEDFCGEWESKRYDL
                     PFEIDGVVIKIDDFVIQKRLGFTAKSPKWAIAYKFKPEIKETTLLEVQFQVGRTGAVT
                     PVAIMEPVYISGSTVSRATLHNEDEIRRLDLHLGDRVRLIKSGEIIPKIIEAVPAHRS
                     ETALAIEFPSHCPACATQLSKDEDGAITYCPNAICPAQMQRRVEHFASRDALDITGLG
                     EAMVQRLLEYDFIKDITGIYRLDYDRLASLEGYGAKSAANLKTAVENSKDRSFDRVIF
                     ALGIRHVGSVTATALAAHFGSIEALMNADIDDLTAIPDVGPRVAQSITAFFSIEENRR
                     LVEELEAIGLNMSWQSSQVSNALDGKTFLITGSLENYGRKEMEAMIVSHGGKILGSVS
                     KQLDYLIVGSKPGSKLAKAQKTEGVKILTEDEALDMMEH"
     gene            10759..12087
                     /locus_tag="PLO35_05240"
     CDS             10759..12087
                     /locus_tag="PLO35_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOH79105.1"
                     /translation="MKILSRYILKEHVAPFFISLLVVTMVLLIDRIIDLLNLIIEKKL
                     AASIVLELFGLSLPYMLALSIPMAVLVATILAFGRMTVDRETIAIKSSGVNVYSMIKP
                     LIVVAILLTGLMVYFNHWFLPETNHKLKNLMIKIAYYKPMTIIKAGEFNQVTDYTVFV
                     KANEGEILRDLLIYDRSQTTFPRLISAESGVVKQMDRGNSLQLTLFNGEMHERGEKDP
                     AKYSLRRFQTYVVNIRNLGNELDIGESGYRSDREMTYGQLRASMKEKGAELKQKRSEV
                     SSLDARIKNLENRPRNYEEDVEYRRLGIMRKMAMDRVSELNQDNRSLQVEFHKKFALS
                     FAIIIFILIGVPLGLMTRSSGIGMAFSVSSIIFLVYYVALNGGENLADKGYMNPFTSM
                     WISNIVFLILALALIWASIKEKSLINLNLLAWRLTHLKSKPSSAPDELIH"
     gene            12095..13186
                     /locus_tag="PLO35_05245"
     CDS             12095..13186
                     /locus_tag="PLO35_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOH79106.1"
                     /translation="MIRKLDRYIIREFLRTYLIIFFSFAVVFIVIDVIDNLPRLMRAG
                     ATTQLAITYYLLRLPFLVVLTSPVTVLLTGLFMMNSLSKHNESVAIRAAGVSIKRAML
                     PLFAIGLLISIGVAVFGEYLLPWAESKRSYVYNVQIKGEQPEDKMLKARVHYQGKNND
                     FYYFGFFDGYQKTLRVIDLVKLDPKTREVTQHLTASAATWNGKNWVLMDCGIRRFSGG
                     RQTGWEYYPNTDKDLLDVEPDDFIRITKKTIALNFMELRDYIARLEKMGEDANREIVD
                     LHMKLAFPLTNLIVIFFFIPVATSNIRSKGRGLIFLLGLVVCFTYLIVVRVIQSLGYN
                     GVIPPIWAAWLPNAAFTLLGFGFLRKAEV"
BASE COUNT         3331 a         3765 c         3474 g         3084 t
ORIGIN      
        1 ttggggtcaa ccgtcatttt gggcatggtt gcgctccttt ccattcgtat ttttttactg
       61 atttcataaa gcctgacagc cccttttttt gtcaaacctt ttcttgtccg ctccgtcgca
      121 gtcagctttg agagcggcag tatcctgcgc gtaatgatga atgccgttat tggagatgaa
      181 atataacttg actaccaggc ggggattgtt aatctgtcta tattcacact gcccgatccg
      241 aaacctcgac agcgtgggat tgggccccat ccttatatat acatggagga tcagatgaag
      301 aatatttgtg taatcggact gctcgtgatc gccttactgg cggcgggatg ttcaacctgg
      361 cggggaaaga aacaacgtcc ggagcttgat ttttatgtgg gaggcgaaac tgccacagcc
      421 tatgagaatt acatacctat cgtggccatg gaagctccca tgccggaaat gaataccgac
      481 gaatttgccg ccatcgagcc caaccgcttt cagagggctg acctgcagcc gctctccacc
      541 ttttccatcg atgtcgacac cggaggctac agcatcgtcc gtaagtttct gaacgacaac
      601 gagctgcctc ctgccaacgc tatccgcagc gaggaactga tcaactactt cagctacgat
      661 taccccaaac cggatggcaa acatcccttc tccgtttaca ccgagctcgc cagctgcccc
      721 tggaacgacc agcaccagct tgttcacatc ggcctcaaag gcatggaact ggaaaccaaa
      781 gaccttccca gctccaatct ggtgtttctg atcgacgtct ccggctctat ggacgagccc
      841 aacaaactgc cgctggtgca aagctcgctc agcatgctca cagatcagct cagagaaaac
      901 gactatgtgt cgatcgtggt ctatgccggg gcggcgggat tggtgttgcc gcccacctca
      961 ggaatgcata aagataagat caagaaggcc ttatacgatc tggaagcggg tggttccact
     1021 gccgggggag aaggtatcca gctggcctat aaaaccgccg cggataacct catcaaagaa
     1081 ggtaacaacc gtgtaatcct ctgcagcgac ggcgacttca acgtgggaat ctccagcacc
     1141 gccgagctgg tggactatgc aaaaaaacag cgggattccg gcatctttat cagcatcctg
     1201 ggctacggca tgggaaacta taaagacaac cgcatggagc agatcgccaa tcagggcaac
     1261 ggcaactatg cctatatcga cgacatcctc gaggccaaaa aggtgctggt gaacgaaatg
     1321 agcggcacct tgctcaccat cgccaaagac gtgaaaatcc aggtggaatt caatcccgcc
     1381 gtggtgggtg cctatcgcct gatcggttac gaaaaccgca tgctgcaggc ggaagatttt
     1441 aaagacgaca ccaaggatgc aggtgaaatg ggcgcgggac atagcgttac agcgctctat
     1501 gagatcatca cgctggagag tggagaaaac ctgccggata ttgactcgct ccgctacgcc
     1561 aaaccagccg tccctgcaga cgccagcacc gcagagatgc tgtttgtgaa actgcgctac
     1621 aagctgcccg acggcgatga aagcatcccg ctggaccagc ctctgccaaa taagtcactc
     1681 ccctggcagg aaagcagcca aaacttccgc tgggctgctg ccgtggcagt ctattccatg
     1741 ctgttgcagg agaatcccaa tcaaggcatg gcggattggg acctggtgat gagcctcgcc
     1801 aaaagcagca aaggcgagga tcccaacggc taccgggcgg aatttattcg cctggccgaa
     1861 aaagcctccc tgatgaagcc aattccaaaa taatcagcct ggagatccca gacccccgat
     1921 caggcttaaa cgctttcccg gagggcattt gggctatgag gccctggcga ggcgttgacg
     1981 tttgtcaaca gccatatcct tcaccaaaac cctccagatg atgaaacagt cgctatatca
     2041 gtcacaacta tctccatgcg gagtagtcag ttaatcttcc tcgtccggaa gtggccccct
     2101 cggaagctcc tctctgagcg caagcgcatc cctctatccc gcaatatctt aaacgctttt
     2161 cgagcttttt tttcttgaca gcatctcccc tctgaaaaat aaggggctca gacctaagca
     2221 aaacaaaacg gaaaaacatt atgagtaatc aaatcaagaa gtggatcgcc acgattttag
     2281 gattacatat gctctttata gcaccagtaa tagctgaaag cgtggccggt ctgggcagcg
     2341 atgagatcat ccaggaccac agtgccctgg ttatcgattc cctgcacacc caggaacccc
     2401 caggaaatcc acctggcgaa cgcatggtag cctcctatta ttccggaaaa ttccatggac
     2461 gtaaaaccgc gagcggcgag cgtttcgacc aatacgccct cacctgtgcc cacaaaaccc
     2521 tcccctttca aaccatcctg aaagttacca atcccaaaaa tggtaaatcc gtgaccgtgc
     2581 gcgttaacga ccgcggtccc ttcacccgtg gcagagatgt ggatctctcc tacgcggcgg
     2641 ccaaagagat cgacatgatc caggccggcg tactcgcggt ggaagtgcta gtgcttccgc
     2701 cctcagaaac taccgaagac gccatagcaa gctcccggtg agtttccctg ggatcaatcc
     2761 ttcctcaggt ggtttgaaga atgattgatc ccattaattt tttctccccc taggtatcgg
     2821 ttctatgaaa acgcatcccg gcattcctct atcctgttct gaagccgtct cacaggccct
     2881 tgagagcaat cagcccattg tggctttgga atccaccgtc atcactcacg gtctccccta
     2941 cccccagaat ttctcgctcc tgcagcgcat ggaagccatt atcacggatt tgggagccgt
     3001 tcccgccacc atcatcgtgt gggagggaaa agcccatatc ggtatcggcc ccaagctgtt
     3061 agacgaaatc aaagccacac taagcctgac ccaaagcgca tttatgaagc ttgggtccag
     3121 ggagctccca ctcgcctttg cccgtggagt taacggaggg accaccgtct cagccactat
     3181 gctgcttgct caactggctg gcattgaagt atttgccact ggtggaatcg gaggcgtgca
     3241 tcgcggctgg caacagagca tggatatttc catggacctg gaagcgcttt ccagtattcc
     3301 tgtaatggtg gtttcggcag gctgcaaggc gattctggac gttccagcca ctctggaaaa
     3361 gctggagacc ctggcagttc ccatcctggg atggcagacg ggctcctttc ccctattcta
     3421 cacagccaca agcccttatc ccatcgaccg ttgtgacagc atcgatcaga tcgtgcgagc
     3481 ctggcgcttt cataaagctt tacaaaccag ctcaagcggc atgctgatcg ccaatcccat
     3541 tccagcggaa cacagcattc catcctctga catcgatccc tatatcgaca ccgctatcga
     3601 agccgccgcc caggccggag cccatggaaa ggccctcacg ccttttctgc ttgactacct
     3661 ggcgcgtgcc acagaaggtg catccataag agccaatctg gccctcctgg aaaacaacgc
     3721 gcgtctggcg gcaaggatcg cgagagcgct aaaggatgat atatgaagct ttatctgtca
     3781 ctggacatgg aaggaatgcc cggcaccttc agttgggaac atgaaaagac agaccgctcc
     3841 gccgtgaaaa gatacttccg cgctcatgtg cagaacgtgc tggagactat cctcagtcac
     3901 ccagcttccg caagcctgga agagattcgc atcgcagatt cccacagcgc cggagacaac
     3961 ctcgattacg acatcacagc cctcgatcca cgtatatcgc tgatttccgg ctcgccgcgt
     4021 cctttatata tgatgccggc tttggaacgt ggttttgacc aggtatggct cctgggctat
     4081 catgccggaa cgggtgcatt gcgcggcaac atggaccaca cctactccaa cagccgcatc
     4141 cacaagatct tcatcaacgg ccaggccatg aacgaagctc tcatcaatgc ggcctatgct
     4201 ggctacatgg gagttcctgt cgctctggta agcggcgaca ccaccttgca ggctgaactg
     4261 cacagcaaag ccgccctgcc gtggattcaa tacgtaccca ccaaaaccgc cgtggccaaa
     4321 ttcgcggccc gcagtttttc atccctgaag gtggcagaat atgttcaaac agctgtcaat
     4381 caagcgcttg tgaccccaaa agctgagctt cctctgtata gatttgatgc tcccatcacc
     4441 ctcaaaatcg agttccactc caccgccatg accgatcagg cttgtctgat gccccacgtg
     4501 aaacgtctgg atggacgcgt gatcgaatac tctgatcccg attacggcgt gatctttgag
     4561 gcgattatgg ccttggtgac gctggccagc accgccaata tgtgaatcct gtaaatagat
     4621 aaaccaacat acttaatgta acgccggaga ttattatgga taactacaag accatagtta
     4681 gatttgaaga tatttttgaa gccgaactgg ccaaatcact gttgatcgac aacggaatcg
     4741 acgccagctt gctcaatgaa cgcatgatgt ccatggcgcc cggtttggca agctccaagc
     4801 tcaatctgga gctgcaggtg ccggaaagcc aggaggccgc cgctctcgag attctggctg
     4861 gctccgtctc cgaaaacgaa accgagaaca tgctcaagag tgaaggcgct atcctggaag
     4921 ggcattttca gctcacttca ggacgccaca gcaacctcta tgtggaaaag atccgtctgc
     4981 tccagaatcc cgccgtggcc aaactgctat gctccaaact ggcggaactg atttctgaac
     5041 atgagtttga ctgtgtagtg ggccccgctt atggcggcat cgctctggcc tttgaagtgg
     5101 cctccctgct ggataaaagc ttcgtgtttt gccagcgcca ggatgggatg atgagcatcc
     5161 gtagcggttt tgacctcagc ggcgttaaac atgctgccgt gattgaagac atcgtcacca
     5221 cgggaggcag cgtcaaagaa gtaatcgcct gcctcaacgc ccgcggtgtg gaagtgagcg
     5281 tggtggccgc cctcgtcgat agaggcagtg gcaaagtgga tttcggcctc cccttcgtga
     5341 gtctgctgca gctcgatatc cccagttggg agccatccga ctgtcccctt tgcgcggaag
     5401 gcatcccaat ccgcaaaccc ggcagcagcg acaaggtaag tatctgaggt agagcacttt
     5461 gcccaggatt gatctgacgg catccactgc ttcggaagcg aagctcctgg ctgaactgct
     5521 gagctttttc gaccctccgg aaggcgtcag gttacgattc ttgtgtcctc ccgaagcccg
     5581 caaagacatt gagctgaagc ataagtggct ggaaatcatc atagataagc ctaaacctga
     5641 gcatcaggtc caggtagtat gttcctcttc cgctggcaat cttgcccgcg aatctctcca
     5701 actatggagt agtaacgctc ttagctgcat agaactgggc cgcgatccga gcctctttaa
     5761 gcaagcccgt gagttctgtg taggctaccg cttttactgt ctggactcgg aacgtctgaa
     5821 tctcaaaggg atagagccct tgcaggtttg gccagagctg tcaaccggca tcgccagtca
     5881 ggcaaaaaca gacatcctcg tgacgcgctc tccggaactg cggcaagcac tgtttatcga
     5941 ctctcccgaa gacctctggc atatcttgat cacagcggaa ggcaatctcc tcgatctgag
     6001 gttggatgat agcgcggaag cctatctgag ggcttatcga cagctgatgg cgttatgcca
     6061 ggcgctctcg gaatctcagc aaatcagtct ggaagccctg cggcgaaact atgataaaat
     6121 tcacggatct tactcctttt tcgcggaatt ctatgaccgc tacatggccc atgtggatta
     6181 ctccgcctgg ctgactatga tcctttcctg ggcgcggagg ttttacgctg gccccctggt
     6241 gagggcgttg gaactagcct gcgggaccgc caatatcgct gagcagcttg tatttaaggg
     6301 catggaagtg gatgcctgcg atctttcgcc ctttatgttg agggaagcgg cccgcaagtg
     6361 tttcaaaccc catctcttca gagcgtcact ggatacgcct ctgccccacc gggactgcta
     6421 tgatctggtt ttgtgccttt tcgacagcgt caattacctg ctcgatattg cagagctgaa
     6481 agccaccctc acacacgtca gagcagccct caagcctggc gctctgttcg ttttcgatat
     6541 ctccaccctg cagaacagcc gtgaaaactt cgacgatctg gttcagcacc accgtttcgg
     6601 taacgccctg atcgtccatc acgccagctt cgacgaactg ggaatgcgcc aaaaatcttc
     6661 cctgacactc taccgcaagc gtcatctggg ctttgtaatg agcgaagagc atcatactca
     6721 gagggtttac cgccaccggg agattctttc cgtgatcgcg tccagccaga tggagcttct
     6781 gggtatcttc aatcccgata cgcgctccaa tcttctcagc aaaaggcatc agaacctcga
     6841 cgagcaatac tcgcggctct tttacatgct cagacgtccc ctatgagcga gctctacaac
     6901 agagacctcg cactcctcac agatcatcct ctccttatgg gagtcgatga agcgggaaga
     6961 ggtgctctgg caggtccggt ggtatgcgcg gcggtgatct tgaactatgg ggagcaaatc
     7021 gaaggcctca atgacagcaa gcttgtaagt gccaaacggc gggatcagct ctacgacaag
     7081 attcttgcta acgctatggc tttcaagatc ataaatatct ctcccgctta tattgatgcg
     7141 cataatatcc tccaggctac tatggaaggt atgaggcagg cgatcgacgg catcgcttgc
     7201 gggcagtgtc tctgcctcat agacggaaac cagcttccct tcaaacactg ccagtcccgt
     7261 catccacagc ttgttcagca gacggtggtg gatggagacg ctcttcatgc ctgcattgcg
     7321 gccgcttcca tccttgccaa agtgactcgt gaccgcttga tgatcgaact ccatccccaa
     7381 aacccctttt atggctttga tcgccataaa ggctacggta ccaccgctca tctgaaagcg
     7441 ctccggcaac atggtccctg tgaagcgcac cgcatgagtt tcggaccgat ccgggaagtt
     7501 ctgcagataa tggatatctt ccatccctga aatagattag ggatatgccg cgccatattc
     7561 ctgtatgtgt tgtgatgccc gtaacccttc tgaactgagc gtgcacttcc cgaacgtcct
     7621 acgggaggtg tgaggtcaga tcagcagagg cataagactc ccgagtaagc ttacaggcgc
     7681 tgagacaaca aagcacttga ccattctgcc ccctctatga gagtggtaaa agtaacgctg
     7741 gctttagccg attgtaacgc tggctttagc cgattgtaac gctggcttta gccggccgta
     7801 gcactgactt tagtcggtgc gtgatgtgat gtctatttgc ttgtatggca tccagttgtt
     7861 acatccagga tggtcgcgtg gggtgccagc tgaagactgg cacaacgacc ggctaaagcc
     7921 agtgctacat attacgtatg atacagataa ttatatgagg agaagagaga tgcgataccg
     7981 ggttgcggtg ctgcagtatg agcccagact tctggagccg caggcaaatc tagaaaagct
     8041 gcttgacatg ctgagaggac tggagacgga ccttgtggtg cttccggagc tggctttgag
     8101 cggatacgtg ttcgccagcc gggaggaagt ggccaaagtg agcgagtcta tccccgatgg
     8161 agcttgtttc caagcactta gagatatctc cattaccagg gatttcagcc tcgtatacgg
     8221 cttcgcggaa cataccaatg gctgtttcta caactcctca gccctgatca atccagacgg
     8281 gagctggcat gtgtatcgca agacgcatct cttcaaccgg gaaaagctgt ttttcacccc
     8341 cggtgatacc ggttttgagg ttcataaagc caagcacgag gtccccatag gcatgatgat
     8401 ctgtttcgac tggcagttcc cggaagcggc ccgaaccctc tctctgaaag gcgcgcggat
     8461 catttgccat ccctccaatc tcgtattgcc ctggtgccag caagcgatga tcacccgcag
     8521 cctggaaaac cgcgtcttca gcatcaccgc aaaccgggtg ggcacagaac gcaacggcga
     8581 tctggaagag accttcaccg gcgccagcca aatcctgagc accaaaggcg agattctggt
     8641 ccgaatggag accaccggca gcggtgtctc tacctgcgag atcaatcccg atctggctgc
     8701 ctccaagaaa gttacagatc gcaatgacgc ttttgccgat cgccgtccgg aattttatca
     8761 gctatgaaca aagatgctct gatatctgag atcagcaggc tgaaagccga aatagagcgt
     8821 cacgacgtcc tctattacca gtttgccaat ccctccatca gcgattatga atacgaccag
     8881 atggccagac gtctcaaagc gctggaagcg gagctaaacg aaactccccc agaggattca
     8941 cccactcaga gggtgggcaa cgatctgaaa caaggcagcg ggacaatccc tcataaacag
     9001 aggatgtaca gcctcgacaa cgcctactcc atcgaggaag ccagagactg gtatcataaa
     9061 ctcagccagg aacttggcgc gcctccggtc tgcgatctgg agctcaagat cgacggattt
     9121 agcatcaacc tctattatca aaacggtcaa cttcaatatg ccagtacaag aggtgacggc
     9181 atcaccggag aagacgtcac cgtaaacgtt aaaacgatag aaagcattcc tgacaggatt
     9241 gagttccaag gctccataga aatccgcggc gagatctata tgcctgtggc ggagttcatt
     9301 gccctcaacg aagccagggc ggccaacgaa gaaaagacct ttgccaatcc ccgcaacgcc
     9361 gcggcgggat ctatcaagct aaaagacagc caggccgtgc gtaagcgcaa actgcaggcc
     9421 ctgttttaca gctatggcta tgccgatccc ctgcccgcgt caacgcaatc tggcctgctg
     9481 gaatggcttc acggccaggg atttccggtt tcggatgcct cccggacggc tgtctccttt
     9541 tcagaaatcg aggatttttg cggagagtgg gaaagcaagc gctatgatct acccttcgag
     9601 atcgacggag tggtgatcaa gatcgacgat ttcgttatcc agaaacgcct cggcttcacg
     9661 gccaaaagcc ccaagtgggc tatagcgtat aagtttaagc ccgaaatcaa ggaaaccact
     9721 ctgctggaag tgcaattcca agttggccgc accggtgccg tcactcccgt tgccatcatg
     9781 gagccggtat atatctccgg cagcaccgtt tcccgcgcaa ctttgcacaa tgaggacgag
     9841 atccgccggc tcgatctgca cctgggagac agagtgcgcc tgatcaagtc tggagagatc
     9901 atcccgaaaa taatcgaggc agttccggcc caccgttcag aaacggctct tgctatcgag
     9961 ttccccagcc actgccctgc ctgcgcgact cagctatcaa aggatgagga cggagctatc
    10021 acatactgcc ccaacgcgat atgccccgct cagatgcaga gaagggtgga gcattttgcc
    10081 tccagagacg cactggacat caccggtctg ggtgaagcga tggtgcaaag actgctggaa
    10141 tacgacttca tcaaagacat cacaggtatc taccggcttg attatgatag acttgcatca
    10201 ctcgagggct acggcgccaa gagcgcggcc aatctgaaaa cggcggtcga aaactccaaa
    10261 gacagatctt tcgacagagt tatctttgcc ctggggatca gacacgtagg ctctgtgacg
    10321 gccaccgcgc tggcagctca ttttggcagt attgaagctc tgatgaatgc cgatattgat
    10381 gatctgaccg cgattccgga tgtgggaccc agagtggcgc aatccatcac cgcctttttc
    10441 tccatcgaag aaaatcgtag actggtggag gagctggagg cgatcggtct caacatgagc
    10501 tggcagagtt cacaggtatc aaatgccttg gacggaaaga cctttctgat caccggcagc
    10561 ctggaaaact acggccgcaa agagatggaa gccatgatcg tctcccacgg tggaaaaatc
    10621 ctcggcagcg tgagcaagca gctggattat ctgatcgtgg gctccaaacc aggttccaag
    10681 ctggccaaag cccagaaaac cgaaggcgtc aaaatcctca cggaagacga ggcgctggat
    10741 atgatggagc actaagcggt gaaaatcctc tccagataca tcctcaaaga gcatgtggcg
    10801 ccctttttca tctcgctgct ggtggtcacg atggtgctgc tcatcgatcg catcatagat
    10861 ctgcttaacc ttataataga gaagaagttg gcagcctcaa tagtcctgga gctgtttggt
    10921 ctgtcgcttc cctatatgct ggctttgtcg attcccatgg cggtgttggt ggccacgatc
    10981 ctggcgtttg gccggatgac ggtggatcgc gagacgatcg ccataaaatc aagcggggta
    11041 aacgtttatt ccatgatcaa gccgctcatc gtggtggcaa tcctgctcac aggattgatg
    11101 gtgtatttca atcactggtt cttgccagag accaatcaca agctcaaaaa cctcatgatc
    11161 aaaatcgcct actacaaacc catgacgatc atcaaagccg gcgaattcaa tcaggtgacg
    11221 gactataccg tgtttgtgaa agccaacgag ggtgagatct tgcgtgacct cctgatctac
    11281 gaccgcagcc agactacctt tcccagattg atcagcgccg agagcggagt cgtgaaacag
    11341 atggacagag gtaacagcct gcagctcact ctcttcaacg gcgagatgca cgaacggggc
    11401 gaaaaagatc ccgccaaata ctctctcaga cgcttccaga cttatgtggt caacatccgc
    11461 aacctcggca acgaactcga catcggtgaa agtggctatc gcagcgaccg cgagatgacc
    11521 tatggccagc tgcgcgcttc catgaaggaa aagggtgccg agctcaaaca aaaacgctct
    11581 gaggtctcat ccctggatgc caggatcaag aatctggaaa accgtccccg taactatgaa
    11641 gaggacgtgg agtaccgcag attgggcatt atgcgcaaga tggcgatgga cagagtgagc
    11701 gagctcaacc aggataatcg ctctctgcag gtggagtttc acaagaaatt cgccctctcc
    11761 tttgccatca tcatctttat tctgatcggt gtcccgctgg gattgatgac ccgctccagc
    11821 ggaatcggga tggcgttctc agtctcatcc atcatcttcc tcgtctatta cgttgctctc
    11881 aacggtggtg aaaaccttgc cgataaagga tatatgaatc cctttacttc gatgtggata
    11941 tctaatattg tgttcctgat tttggctttg gcgctcatct gggcctcgat caaggaaaag
    12001 agcctcatca acctcaactt gctggcctgg cggctcacac atctcaagag caagccaagc
    12061 tccgcgccgg atgaattgat ccattgaggc agccatgatc cgtaaactcg accgctacat
    12121 catccgtgaa ttcctgcgca cttatctgat cattttcttc tcattcgccg tggtctttat
    12181 cgtcatcgac gtgatcgaca acctcccaag actgatgaga gcgggcgcta ccactcaact
    12241 ggcaataacc tattaccttc tcaggctgcc gtttctggtg gtgctgacct ctccggtaac
    12301 ggtgcttctg acgggacttt tcatgatgaa ttcgctctcc aagcataacg agagcgtcgc
    12361 tatccgggcc gcgggagtga gtatcaagag ggccatgctg ccactgtttg ccatcggtct
    12421 gttgatcagc atcggggtgg ccgtgtttgg agaatatctc ctcccctggg cggaatccaa
    12481 acgcagctat gtgtataacg tgcagatcaa aggagagcaa ccggaagata agatgctgaa
    12541 ggcgcgggtg cattatcagg gtaagaacaa cgatttctat tacttcggct tctttgacgg
    12601 ttatcagaaa actctgcggg tgatcgatct ggtgaagctt gaccccaaaa cccgggaagt
    12661 aacccagcac ctcactgcca gcgccgccac ctggaatggc aaaaactggg tgctgatgga
    12721 ttgcggcatc cgaagattct ctggtggcag acagacgggc tgggagtatt atcccaacac
    12781 cgataaagac ttgctcgatg tggagcccga cgacttcatc cgcatcacca aaaagactat
    12841 cgcgctgaac tttatggaac tgcgcgacta tatcgccaga ctggaaaaaa tgggcgaaga
    12901 cgccaatcgc gaaatagtgg acctccacat gaaactggcg ttcccgctca ccaacctgat
    12961 cgtgatcttc tttttcatcc cagtggccac atccaacatc cgctccaaag gcagaggcct
    13021 catcttccta ctgggactgg tggtctgctt cacctacctg atcgtggtga gagtgatcca
    13081 aagccttggc tataatggcg ttatcccacc tatctgggct gcctggctgc cgaatgccgc
    13141 gttcacgctt ttggggtttg ggtttcttag aaaagcggaa gtgtagaagg gtgagatggc
    13201 ttcgccaggt gagatacctg cggtaggtga gatacctgcg gtaggtgaga tacctgcggt
    13261 aggtgagata cttcgtaggt gagatacttc gtaggtgaga tacttcgtag gtgagatact
    13321 gcgtaggtga gatactgcgt aggtgagatg ctgcgcatgt gagagctgcg cgtgtgagct
    13381 cttcgagctg gtgagagccg ttccggctgg tgagagcact gcagctggtg agcatgcatg
    13441 tagattcctg gtattcacaa atcaaatcct gtaaatcctg tcatcgtgtc atctgcgttc
    13501 tatttcaaat ccgtgtaaat cagttttatc tgtgtcatct gcgttctata actcctcagt
    13561 tctcaattaa tcataaatcc tgatcatccc ctttttccct ttttctgcgt tccattaatt
    13621 ctcaatcccc aaaaagctat tgacagattt ttag
//
LOCUS       DAOSFN010000060        13580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3767_length_13580_cov_9.230610, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000060 DAOSFN010000000
VERSION     DAOSFN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13580
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3767_length_13580_cov_9.230610"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLO35_05250"
     CDS             complement(<1..346)
                     /locus_tag="PLO35_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOH79107.1"
                     /translation="MKSDCCDYLDFGRLSFNRKTMRDKLPQDVFFALQQTIENHQPLD
                     PALANAIAHGMKEWAMENGATHYTHWFQPLTGFTAEKHDSFITADRDGTVFARFSGNQ
                     LIMSEPDASSFPS"
     gene            complement(494..1183)
                     /locus_tag="PLO35_05255"
     CDS             complement(494..1183)
                     /locus_tag="PLO35_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOH79108.1"
                     /translation="MTLVAALLLCPYLWGEAAKALDTDVYTPESDVPLPCVVICPGRG
                     YHKDLPLVKDLASRLKEEGFATLSFNWSFFTAKTQPSQDGSAELADIDAAIEMAKGIP
                     GVDSSRIYLVGKSLGSVYGYHAFQKHPELKACLLYTPVIPEPDLGSVYYPDLKDESRP
                     VAFILGNKDQDNCPLGNLYAYLAGITRDIPLVVLSGGHSFEESDDYTAPINVYNTKLA
                     IEMGVYWLKRM"
     gene            1450..1671
                     /locus_tag="PLO35_05260"
     CDS             1450..1671
                     /locus_tag="PLO35_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79109.1"
                     /translation="MHKYLIAAIEWPIIALALLLIWEGFGLQGWVGIEALGGLFLAFV
                     GFELHLIYHRKEREENQRKKQEPLKKKPK"
     gene            complement(1699..2256)
                     /locus_tag="PLO35_05265"
     CDS             complement(1699..2256)
                     /locus_tag="PLO35_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79110.1"
                     /translation="MKQILSVILIILLLGVSGRLFAETQTSPALSMADFDPPPKTHLI
                     SGFWITGKALSDFDINLKGLMAESTLLIMDKFCVSGFYLDREGFDIPFYQTKAYGVLV
                     GYCDTVRDRMPIRGSISVGVSHNNSDSAHSEKTYYGIPIKLDATAIYGNFLGFTIGIL
                     VDINNHSSYGNIFYGIAIGSFGVRK"
     gene            complement(2381..7381)
                     /locus_tag="PLO35_05270"
     CDS             complement(2381..7381)
                     /locus_tag="PLO35_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79111.1"
                     /translation="MKLYAVLLFLLAGMLGNLMADVYHSTTMGGWWMDPNTWQSTGYP
                     DADDDVFIHGIVFVIGENSCNHLDIQGSDAMIKCGDNASGTLTVHGNLTTSGWVTSSA
                     SYNLTLNLYGNLSINYNFVPHNFNWLGTGVKTLSCGSSSFGLRTRNITSISASIEAIQ
                     AISDIYFLPGGTYTPVIKGNGGHVDVRLFDPETRTTYNLNSRSCRLEYLKIQGDGSSE
                     FNFNNDSNLGNVWMSYCELENLLTSGTQMFDNGCSVTDITNNGSIYNAASGSRDLYVY
                     GPFTNNGQVGQAPHGYNFNVYSYGDISNTGLFRPSNLYLRGTDARTLFSTAANPIQAS
                     ISMTGEAELGNVYAGDQLHFANINNVTGPLNFKAFSQSNQARTLTFNNVKIYSAVIEG
                     IAGSSLNGTDLLLQNTPISNLDLQGSLFFNAPASLTNVVNHGTLQNYNSSGSVTIAVY
                     GDFANYGSVANYGGMYFTVNAWDDVRNYGTWTAYRLNLYGDDAQEICFGAAHPFAGSY
                     CYDMDPTSSIYVVEEDLYINCPYMDLNYSTLYLNPGGFDLHLSGTELIEANIVSELGN
                     ILNMSNNARLNNVGFQSVTNAGTMNMTTTTSFSGSLINNGTVQDWGASVSLSVAGDIT
                     NNGSICNSGGYLLTLNGGGNLINNGSWTNYSTRLNSPNPQIIHFPEEHPYRGSYFYDL
                     NADSSIQSDADLWFVNTTTIDSNYSIWRLDLGNWDLHLDNCIMVETYIHATEASVITM
                     VNGGHLTSCTFQSITVEGTLNLNSNTPFSGNLINNGMIQNHGNSITLSVTGNCVNNGT
                     IRSNEGYHLYVHLGGDAINNGNWGGYSLTLNGSTTQLIHFPSGHGFTGSLFNDNNSAS
                     SVVADSDQYITNATIDLNSSPLQLTGGHDLWLDNCILVDVPVLSGQSSQLSMVNGGYI
                     SNCSFQGITMNGTISLNSETSVSETLVNLGILQSHTSSVSLIISGGLDNQGTIRNNPG
                     GYHLYLYCGQNIVNSGVISTHHLYLTGASPQSISSNGSIGCSNVTDNNAISSVTLLTD
                     LQLSANVDFNGATLILNDGSRIGKTLTLSGGYFIEATVVGGNTSRLVTGNGNWMSSLA
                     FDDIIWEGVVTLNSSVSVNNLVNRATVQCTGNSSATLAVNGRLDNMAEGVFQNNNYNL
                     TLNCYGDIYNHGQLRNYDIYFRSGENQSIWQSASADTIRCGHLRKTNTTGTVTMLSNL
                     FLKNCHITFNCQDLIMESGRTVFSLSLYGSYLESIDLVSTGYATLNLAGGAYIGSGVT
                     GANMIWTGYLPLISNTTINNLINQGVIQNQSGYYGYLYVNGRLDNYGTISNASSYPLD
                     VYSYGDVYDYGNIGTRYFYFNGTGTQAIFQSPEADVISSAAFRKTVGSGDVMMLSDLR
                     LQNCSINLNTRSLFMNGGGMDHTLYMSGAHIENTYLASPGNATLDFSLVTRLNNINGG
                     NLTLRGTVYIYGTCNFGSIINYGVTRNLNNEYAHLYVNGDLVNHGTFHCDGYPLYIYV
                     TGHVTNNGTMAHRRVHITGTADQNVQLNGSETIDALRLQSNIGSAVWYRDGSPSGLTG
                     SYIDLAMNNPLLMGVWQAYVTSTNTWDRIITVSPVGTLNAPENLSISLSGDDLLLRWN
                     QVNSASSYTIFTCETPEGVFSVLMSGITDPNPGDGIVEQTLPPSSQRRFYRVTASN"
     gene            complement(7670..8515)
                     /locus_tag="PLO35_05275"
     CDS             complement(7670..8515)
                     /locus_tag="PLO35_05275"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOH79112.1"
                     /translation="MPEKPSSILIKKASGHTEPFDTSKLRASLKNAGARAEAIDAVVA
                     EIESWVYDGVSTSQIYSRAFKIYRQLSTSGALLYRLKQAILEMGPSGYPFEHFVGELF
                     KRQGYQVEVGIVMEGASVHHEMDVVASKDRKLILGECKYSHKQGFSVSVQVPLYVHSR
                     VNDIVDKLLQQQQYKGFTFIPWIFTNGRFTSDSTQYSHCKGINLMSWDHPRGQALKDL
                     IEKERMFPITILSNLTKSQKSKLLMEGIVTCEQLLKHSDKLDDEGLEKTKRAVLKELE
                     ALAEI"
     gene            complement(8659..11142)
                     /locus_tag="PLO35_05280"
     CDS             complement(8659..11142)
                     /locus_tag="PLO35_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOH79113.1"
                     /translation="MLKYTFTSLVLMLLVTLLTATQLDGAGFGSQDYAHDLIKIKLSR
                     EAVEMADLPDLPYASNPVFGEQELDKILALTGGIAVIRAHIRPKDRIWEAATGFDRWF
                     LIRLNGKTTVEVALAAFEQSPLVEFAQPEYLAYLSDMPNDPLNSLNWGHQNTAQLPAW
                     SPPGFSGGSHSGTPVGLVGFDSNIIPAWDQAQGYGSQDIVIAVIDSGVDINHPDLDLV
                     TGYDYGDNDPDPTDTNGHGTAVSGIVAAIADNGIGAAGVAGNTKVMPLKVLNSANALL
                     FTNIANAVIHAADNDADLINLSLGGAGSEGANPSMDAALEYAYSNAGIPIFASTGNNN
                     QSTILYPSNHNKVISVGAATPTGGRKTTITQDGEYWWGSNYGSTVQDSPTAIDIMGPT
                     ILPTTDILGAGGYSNSDYYSWFNGTSCASPYVAGVAALLLSRDPSLSPAEVREALVNS
                     ATDMMPEGGTGWDRFTGYGLVNANAALNYIADPAQITWAPTSIETTLAAGQTASIPIQ
                     IGNAGENLLEFSLSRTPIVETAFSEGFENNGYESQGWSSVINSGNQHWLWGSGGAYGS
                     NPSSAYEGSINARFFNAGSSVVSASLISPPLDLSGVASATLSFYHTQAYRFGQDRLTV
                     AYRNSASGPWTTLAFYTGNIEAWTQRTIELPNLSSEYFLAFSGFCPSSAGWGVTLDAV
                     SISVVSGYEAPWISIAAGTSQIGEISSGNSLNLSVDLDASDLEEGTYTSNLLLSSNSS
                     PNPLVQIPVTLTVGANAALGSPQVSISQSSGVILLQWPVVPNAARYLVYRADQALGDY
                     DLIAETAGLQHQISAGPDMGFFRVTASNP"
     gene            11445..13469
                     /locus_tag="PLO35_05285"
     CDS             11445..13469
                     /locus_tag="PLO35_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOH79114.1"
                     /translation="MQYPTTRKDDIKEESFGTLVQDPYRWLEDDHSDETIAWLKAQQE
                     LTESYLNSFPGRKAMLERVRALNDYPKQTCPTKHGDWYYHYKNDGLQNQWVMWRRKAG
                     EETEELFFDPNTISEDGTTRAYPIGWSKDYRYVCFNYSQAGADLGEFRIMDLATKEFL
                     PDVLKDMRHTNVAWYKDGFFYGRYDKSQDYQAQDCDQKIYFHRLGDPVSEDKLVFEDP
                     EHPLRFLHTYVSDDEKTLFLYQSQGTHGNQVHFRPAGDDKAPWTLLFEGFEYDGWPTD
                     GFEEGIFYLFTNKGAKNFRLLKVDLTKPQEDNWIEIIPERDYPLDSVIPVGGKLIAIY
                     TKDVQSQIEVLDADGKFLYPIQMPYQGGTQFTCGRSTDREGYFYFESFIKPSESYHYD
                     VDTNTLSFYYRDPVPAELDALVSKQIFFSSKDGTRVPMTLIYKEGLQLDGSHPLLLYG
                     YGGFNISLQPGFSASRIALIEQGFIYVVVNLRGGGEYGENWHEQGMLLNKQNVFDDFC
                     AAAEYLIREGYTSADRLAINGGSNGGLLVGACLTQRPELFRAAVPSMGVLDMLRFHKF
                     TCGWGWMVEYGDPGQEEQFRNLLSYSPLHNIKPGVKYPATLVTTADHDDRVIPGHSFK
                     FAATLQEHADPARPVLLYTQFSSSHGPSNLNKTLELWADIWSFLMLELKR"
BASE COUNT         3173 a         3375 c         3678 g         3354 t
ORIGIN      
        1 cgctgggaaa cgaagaggcg tccggttcac tcatgatcag ctggtttccg ctgaatctgg
       61 caaagacggt cccatccctg tctgcggtga taaatgaatc atgcttctct gccgtgaatc
      121 ctgtaagggg ttgaaaccaa tgggtatagt gggtcgcgcc gttttccatg gcccattctt
      181 tcatgccatg agcgatggca tttgccagag cgggatcgag gggctggtga ttttctatgg
      241 tctgttggag cgcgaaaaag acatcctgag gcagtttgtc gcgcatggtc ttgcggttga
      301 atgacaacct gccaaaatcc agataatcgc agcagtctga tttcatacac atacctctat
      361 atgtttatac agaagggctc aaagtctggc aatactaaga agcgtcaagc actttctggc
      421 ttcaggttgc aactgattaa ctggcaggag attgccagaa gcgaacctgg aatctaccct
      481 gtgaagatga gggttacatc cgtttcagcc agtaaactcc catctctata gctagcttgg
      541 tgttatagac gtttatgggg gcagtgtaat catcagactc ctcaaaggaa tgacctccac
      601 tgagcaccac cagcgggatg tctcttgtta ttcccgcaag ataggcgtag aggttgccca
      661 gtgggcagtt atcctggtcc ttgttaccca gaataaaggc cacgggacgc gattcatcct
      721 tcaagtcggg ataataaaca gagcccaaat ctggttctgg aatgacagga gtgtagagca
      781 ggcaggcttt gagttccgga tgtttttgaa aggcatggta tccatacact gagcctaaag
      841 acttgcccac cagatagatt ctgctgctat ccactcctgg aatgcccttg gccatctcaa
      901 tcgcggcgtc gatatcagcc agctcagcag agccgtcctg agagggctgg gtcttggcgg
      961 tgaaaaagct ccagttaaag ctcagcgtgg caaagccttc ctccttgagc ctcgatgcca
     1021 ggtcttttac caggggcagg tctttgtgat agcctctgcc cggacagatc actacacagg
     1081 gtaacggcac atctgattcc ggggtatata catcggtatc cagtgccttt gcggcctcac
     1141 cccagagata ggggcataat aacagcgcgg ctactaaggt cattaacagc ctcttaggta
     1201 gtgatttcat agggctgact cctgtaatgg tttgtatcca aagtaccttt gcttcagaca
     1261 tcagaatacg gttttagcgg gtgtaatcct gtcaaggtct ttctggctca gactgattcg
     1321 accaagctgc tcaataacgg cgatgcatga atatgcttat cgggcagaca catgatatca
     1381 aacaattcat ttgacagtcg atcccacagc agtaggctgg cacgtacagc aatcatcagg
     1441 agacacatca tgcataaata tctcatagcc gccatcgaat ggcccatcat cgctttggca
     1501 ttactgctta tctgggaagg ctttggcctg cagggctggg tgggcatcga ggccctggga
     1561 ggtctgtttc tggcttttgt gggctttgaa ctgcacctca tctatcatcg caaggagcgc
     1621 gaggaaaatc agcgcaaaaa gcaggaacct ctcaagaaaa aacccaagtg attttctcaa
     1681 tctcctgctg gaatatactc acttacggac gccaaaactt ccaatcgcta tcccgtagaa
     1741 tatgttacca taggagctat gattgttgat atccaccagg attccaatcg tgaaacctaa
     1801 gaagttgccg tatatcgcgg tagcatcgag cttgattgga atcccatagt aggttttctc
     1861 gctatgggca ctatcagagt tgttgtgtga aacgccgaca gaaatcgagc cccggatggg
     1921 catccgatcc cgcaccgtat cgcaatatcc taccaagact ccatacgctt tggtttgata
     1981 gaagggtatg tcaaatcctt ccctgtccag ataaaaacct gaaacgcaga acttgtccat
     2041 aatcagcaag gtcgattcag ccataagacc tttcaaattg atatcgaaat cagacaatgc
     2101 ctttcctgta atccagaaac cgcttatcag gtgggttttt gggggaggat cgaaatccgc
     2161 catactaagt gctggtgatg tctgggtttc agcgaacaac ctgccagaca ctcccaggag
     2221 aagtatgatc aagatcactg acagtatttg cttcattaag cctcctaaaa gaacgaaact
     2281 cagttattgt gaagttatgc cgggtcgccg gcatccaggc agaaacgtag atcaatacca
     2341 aactgatacc tgattgaatc cattagtaac agccaggact tcagttactg gccgtcaccc
     2401 gataaaacct tctctgtgag ctgggcggca gggtctgttc cacgatcccg tccccgggat
     2461 tgggatctgt gatcccgctc atcaatacgc tgaaaacacc ctcgggagtt tcacaagtga
     2521 atatggtata tgagcttgcg ctgttcactt gattccatct cagcagcaga tcatcaccgg
     2581 acaggctgat gctcaggttt tcaggcgcgt tcaaagtacc tacggggctg accgtgatga
     2641 tcctatccca ggtatttgtt gatgtcacat aggcttgcca tacccccatc agcagaggat
     2701 tgttcatcgc caggtctatg tagctgcccg tcagtccgga aggagaaccg tcgcgatacc
     2761 agacggctga gccgatattg gattgcagcc tgagggcatc gatagtctcg ctgccgttca
     2821 gctgaacgtt ctggtccgct gtgccggtga tatgcactct ccggtgggcc attgttccgt
     2881 tgttggtaac gtgcccagtc acatagatat agaggggata gccgtcgcaa tggaaagtgc
     2941 cgtggttgac gaggtctccg tttacataga gatgggcgta ttcgttgtta aggttgcggg
     3001 taacgccata gttgatgata gacccaaagt tgcaggtgcc ataaatatac acggtacctc
     3061 ttaaagtcag attgccgccg ttgatgttgt tcagccgggt taccagtgaa aagtcgagcg
     3121 tggcgtttcc aggtgaggcc agataagtgt tttcgatatg agctccactc atatacaggg
     3181 tatggtccat cccgccgccg ttcatgaaca ggcttctggt gttgagattg atggagcagt
     3241 tttgcagcct caggtcggaa agcatcatca catctcccga tcccacggtt ttgcggaagg
     3301 ccgcggagct tataacatcc gcttccgggg actggaaaat tgcctgggtg ccggtaccgt
     3361 tgaaatagaa gtaacgggtg ccgatattgc cataatcata aacgtctccg taggagtaaa
     3421 catctagcgg gtaagagctt gcgttgctaa tcgtgccgta gttatccagg cgaccgttca
     3481 catacagata gccatagtaa cccgactggt tctgaatgac gccctgattg atcaggttgt
     3541 ttatcgtcgt atttgagata agcgggagat aaccagtcca gatcatgttt gcgccggtga
     3601 cgccggagcc tatgtaagcc cccccagcca ggttgagagt ggcgtatccg gtagatacca
     3661 ggtcgatgct ttcaaggtat gaaccataga gagacagcga gaaaactgtc cgcccgcttt
     3721 ccatgatcag gtcctggcag ttaaaggtga tgtggcagtt cttcaggaag agatttgaga
     3781 gcatagtcac tgtgccagtt gtgtttgtct ttctgaggtg tccgcagcga atggtgtcag
     3841 cggaagcgct ttgccagatg ctttgatttt ccccggagcg gaagtagatg tcataattgc
     3901 gcaactgacc gtgattgtag atgtcgccat agcagttgag ggtgaggttg tagttgttgt
     3961 tttggaacac gccttcggcc atgttgtcca gtctgccgtt gacggcaagg gtcgctgagc
     4021 tgttgccagt acactgcacc gtggcgcggt tgacaagatt gttcacgctc acgctggagt
     4081 tcagagtgac aacgccttcc cagatgatgt cgtcgaaggc cagagagctc atccagttgc
     4141 cgttccctgt taccagtctc gatgtattgc ctcccaccac cgtagcctca ataaagtaac
     4201 ctccagacaa cgtgagtgtt ttaccaatgc ggctgccgtc gttgaggatg agggtcgcgc
     4261 cgttgaaatc cacgttggcg ctcaactgca ggtctgtgag cagggtcacg gaactgatgg
     4321 cattgttgtc ggttacattg ctgcaaccga ttgatccatt gctgcttata ctttgtggtg
     4381 acgctcccgt caaatagaga tgatgagtgc tgatcacacc ggaattgacg atattctgtc
     4441 cgcaatacag atagaggtgg tagccacccg gattgttgcg gatcgtgccc tggttgtcca
     4501 gaccacccga aataatcaaa cttactgagg aggtgtggct ttgcagaatg cccaggttca
     4561 ccagggtttc cgagactgag gtttccgaat taaggctaat tgttccgttc atagtgatgc
     4621 cctggaaact gcagttggag atataacctc cattcaccat gctcaattgt gagctctgac
     4681 cagacagaac cggcacgtcc accagaatgc aattgtcaag ccacaaatca tgcccgccgg
     4741 ttagctgtaa ggggctgcta ttgaggtcta tcgtggcatt agtaatgtac tggtcgctat
     4801 ctgcaacgac gctcgacgcg gagttgttgt cattgaataa ggaaccagta aaaccatgtc
     4861 cagaagggaa atggattagc tgtgtagtgc tcccgttcag agtgagagag tatcctcccc
     4921 aattgccgtt gtttatggca tctccaccca gatggacgta gagatggtat ccctcattac
     4981 tgcgtatcgt gccgttgttg acgcagtttc ccgtcacgct gagcgtgatg ctgttaccat
     5041 ggttttggat catgccgtta ttgatcagat ttccgctgaa gggggtgttg gagttcagat
     5101 tcagggtccc ctccacagtt atggactgga aagtgcagct ggtcagatgg cctccgttta
     5161 ccatggtgat gacactggcc tcagtggcgt ggatataggt ttccaccata atgcagttat
     5221 ccagatgaag gtcccagttt cccaggtcca gcctccagat ggagtagttg ctgtcgatcg
     5281 tggtggtatt tacaaaccag agatcggcat cgctctggat ggatgaatca gcattcagat
     5341 catagaaata ggaaccccga taggggtgct cctccgggaa gtgaatgatc tggggattgg
     5401 gagaattgag cctggtgctg tagtttgtcc aactgccgtt gttgatcaag tttccaccgc
     5461 cattgagggt gagcagatag cctccggaat tacagatgga gccgttgttg gtgatatcgc
     5521 cggctacgct gagggatacg cttgcccccc agtcttgcac ggtgccgttg tttatcaggc
     5581 tgccgctgaa gctggtggta gtggtcatat tcatggtgcc ggcgttggtg acggattgaa
     5641 atcccacgtt gtttagccgc gcgttgttgc tcatattcag gatattgccc agttcggaga
     5701 cgatattggc ttcgatcagc tcggtgccgg agaggtggag gtcaaaacca ccaggattca
     5761 ggtagagagt ggagtagttc aggtccatgt aggggcagtt gatatatagg tcttcctcca
     5821 ccacatagat gctggaagtt gggtccatat cataacagta ggatccggca aagggatgag
     5881 cggctccaaa acagatttcc tgagcgtcat cgccatacag gttcagccgg taagccgtcc
     5941 aggtgccata gttgcgcaca tcatcccaag cgtttaccgt gaaatacatc ccgccataat
     6001 tcgcgacgct gccataattt gcgaagtccc cgtaaacggc gatggtgaca gaaccgctgg
     6061 agttgtagtt ttgcagggtg ccgtgattca ccacattggt gaggctggca ggggcgttga
     6121 aaaagaggct gccctgcagg tccagattgc ttatgggagt gttttgcagc agcaggtctg
     6181 tcccgttcag gctggagccc gcaatacctt caatgacggc agagtatatc tttacgttgt
     6241 tgaaggtgag cgtcctggcc tggttagact ggctgaaagc tttgaagttg agcggaccag
     6301 tgacgttgtt gatattggca aagtggagct ggtccccagc gtaaacgttt cccagttctg
     6361 cttcgccggt cattgagata gaggcctgaa tggggttggc ggcggtgctg aaaagcgtcc
     6421 tggcatcagt gcctctcaga tagaggttgc tgggtctaaa gaggccggtg ttgctgatgt
     6481 ctccgtagga gtatacgttg aaattatatc catggggcgc ttggcctacc tggccgttat
     6541 ttgtgaatgg accatataca tacaggtccc ggctcccgga agcggcgttg tagatgctgc
     6601 cgttgttggt gatatccgtg accgaacagc cattgtcaaa catctgagtg ccggatgtca
     6661 gcagattttc cagctcacag taagacatcc agacattgcc gaggttgctg tcgttgttga
     6721 agttaaactc gctgctgccg tctccttgga tctttagata ctccagacgg cagctacgag
     6781 agttgaggtt gtaggtggtt ctggtctcag ggtcaaacaa cctcacatcc acatgacctc
     6841 cgtttccttt gatcacgggt gtgtaagtcc ctccaggcag gaaatagata tcactgatgg
     6901 cctggatagc ttcaatactt gcagaaatgg aggtgatgtt tctggttctc agcccaaagc
     6961 tgctactgcc gcagctcaaa gtttttacgc ccgttcccag ccagttaaag ttatggggca
     7021 caaagttata gttgatgctc agattgccgt agagattcag ggtgagattg tagctggcgc
     7081 tgctggtcac ccagcctgaa gtggtcagat tgccatggac ggtcagagtc ccgctggcat
     7141 tgtctccaca cttgatcatg gcgtccgaac cctgaatatc caaatggttg caggagttct
     7201 ctccgatcac aaatacgatg ccgtgaataa acacgtcatc gtcggcgtcc ggatatccag
     7261 tgctttgcca ggtgttggga tccatccacc atccacccat tgtggtagaa tgatacacat
     7321 ccgccatcag atttcccagc atgcccgcca gcaagaaaag caagactgcg taaagtttca
     7381 tgacactcac ctcgttgtaa gaattgcctg aacctttggt aatcgattcc cggaaagatg
     7441 tcaagcattt tatgcgattg tcaatgccga caatccggaa tcaaagatgc atttcttcca
     7501 cagcgggctg atgatccggc ttctcattcc cgggttctga tctcctgtgc ccctcccgtg
     7561 cctctccttt acccaggcaa aggagaggca taggagaggc ataggtggtg cataggtggc
     7621 tgattgggag ggttgcttga aaatacgtgc ccgagtgaga ctagcagcct cagatctcag
     7681 ccagcgcttc cagctctttc aggactgcgc gtttggtctt ttccaagccc tcgtcatcca
     7741 gcttgtcgga gtgcttcaac agctgttcgc aggtgacgat cccttccatt agcagttttg
     7801 acttctgact tttggtgagg ttgctgagga tcgtgattgg gaacatcctt tccttttcta
     7861 tcaggtcttt cagggcctgg cctctggggt ggtcccagct catcagattg atgcctttgc
     7921 agtggctgta ctgggtggaa tctgaagtga aacgcccatt ggtgaagatc caggggataa
     7981 atgtgaaacc cttatactgc tgttgttgga gcagcttatc cacaatgtcg ttgacgcggg
     8041 aatggacgta tagcggtacc tggacgctca cgctaaagcc ttgtttatgg gaatacttgc
     8101 actctcccaa gatgagtttc cggtccttgg aggccacgac gtccatttcg tggtgcacgg
     8161 aagcgccttc catcacgatc cccacttcca cttgatagcc ctggcgcttg aaaagctcgc
     8221 ctacaaagtg ttcaaaggga tatccggagg ggcccatctc gaggatggcc tgtttgagcc
     8281 ggtaaagcaa agcccctgat gtgcttagct gccggtagat cttgaaggcg cgggagtaaa
     8341 tctgggaagt gctgaccccg tcatagaccc agctttcgat ctcggcaact actgcatcga
     8401 tggcctctgc ccgggccccg gcgttcttga gcgaggccct aagtttgctt gtgtcaaatg
     8461 gttcagtgtg accggaggct tttttaatga gaatggaaga gggtttttca ggcatatctg
     8521 gctccttgcg ttatagagcc aatataactg gaatcctgag ataagccaga gccagaaccc
     8581 cgggacccaa ccaggatccc gggatggcaa agagcgatat caagccggtt tatgtgggag
     8641 gattgggccg gcagcgtttt aggggtttga ggctgtcacc cggaaaaatc ccatgtctgg
     8701 acctgcactg atttgatgtt gcaaaccagc cgtctccgca atcaagtcat aatctcccaa
     8761 ggcttggtcg gcacggtaga ccagataacg ggcagcatta ggaacaacgg gccactgcaa
     8821 taaaataact ccagagcttt gtgatatgga cacctgaggg gatcccaacg cggcattggc
     8881 gccaaccgtc aaagttacag gaatctgcac caaaggattg ggggaggaat tgctgctgag
     8941 cagcagattg ctggtgtagg tcccttcctc cagatcactg gcgtccaggt ccacactcag
     9001 attcaggctg ttgccagaac taatctcacc gatctgagag gttcctgcgg ctatgctgat
     9061 ccagggtgcc tcataaccgc ttaccacgga tatagatacg gcgtccagcg tgacacccca
     9121 gcccgcgctg gaagggcaaa aacctgaaaa agccaggaag tattcggaac tgagattggg
     9181 gagttcgata gttcgctgag tccaggcctc gatgttgccg gtatagaagg ccaaggtggt
     9241 ccagggccct gaggccgagt ttctgtaagc cactgtaagt ctgtcctgcc caaacctgta
     9301 agcctgtgtg tgatagaagc ttagggtggc ggaagctact cccgacaggt ccagcggagg
     9361 gctgatcaat gaggccgata ctactgaaga ccccgcgttg aagaatctag cattaatgct
     9421 gccttcatag gccgaggaag gattcgagcc gtaagctcct ccgcttcccc acagccaatg
     9481 ttgattgccg gagttgatca cgctgctcca gccctggctt tcataaccgt tgttttcaaa
     9541 tccttctgag aaggccgttt ccacaattgg agtgcggctg agcgaaaact ccagcagatt
     9601 ttctccggcg ttgccgatct gaattggtat cgaggcagtc tgcccagccg ccagcgtggt
     9661 ctcgatggaa gtgggggccc aggtgatctg ggctggatcg gcaatataat tcaaagcggc
     9721 attggcattt actaaaccgt atccggtgaa acggtcccag ccggtaccac cttcgggcat
     9781 catgtccgtg gcgctgttaa ccagggcttc acgcacttct gcggggctta gagaagggtc
     9841 tctggataac agcagcgccg ccactccagc cacataagga gaggcgcagg aagtgccgtt
     9901 gaaccagctg taatagtctg agttgctgta tcctcccgcg cccaggatat cggtggtcgg
     9961 caggatggtg gggcccatga tgtcgatcgc ggtaggagag tcctggaccg tcgatccata
    10021 gttcgatccc caccagtatt cgccatcctg agtaatggtg gtttttctac ccccggttgg
    10081 ggtcgcggca cccactgaga tcactttgtt gtgattggag gggtagagaa tggtggattg
    10141 attgttgttg ccggttgagg caaaaatcgg gattccggcg tttgaatagg catattccaa
    10201 agcggcatcc atggatggat tagcgccttc tgatccagcc ccgcccaggc taaggttgat
    10261 caggtcagcg tcgttgtcgg cggcatggat aacggcgttt gctatattgg tgaagagcaa
    10321 ggcattggcg ctatttagca ccttaagggg catcactttg gtattgccag ccactccggc
    10381 tgcgccaatt ccgttatcgg cgattgcggc gactatacct gaaacggcag ttccatgtcc
    10441 gtttgtgtcg gtgggatcgg gatcgttgtc gccataatcg tagcccgtta ccagatccag
    10501 atcgggatgg ttgatatcca caccgctgtc tatgaccgcg atcacgatgt cttgcgagcc
    10561 gtagccctgt gcttgatccc atgccgggat gatgttactg tcaaaaccca ccaggcctac
    10621 tggagtcccg gagtggcttc caccactgaa tcctggtggt gaccaggcgg gaagctgagc
    10681 tgtgttttga tggccccagt tgagtgagtt caggggatca tttggcatat cgctcagata
    10741 ggccagatat tctggctggg caaattccac cagggggctt tgctcgaaag cggcaagtgc
    10801 cacttccact gtggttttgc cgttcaggcg gatcagaaac cagcgatcga atccggtggc
    10861 agcttcccag atcctgtctt tgggacggat gtgagccctg atcaccgcta ttccacctgt
    10921 cagagcaagg attttatcca gttcctgctc tccgaaaacc ggattgcttg cgtatgggag
    10981 atcaggaaga tctgccattt ccacagcctc tctgctcagt tttatcttga tcagatcatg
    11041 agcgtagtcc tggctgccga agcccgcgcc atccaattgc gttgccgtca gcaatgttac
    11101 cagcagcatc aaaacaagcg aagtaaacgt atattttaac atgaggtact cctattaaat
    11161 tgctaggtga ttcatataat aatccagcgt caggttccag gcaagaggat actgataatc
    11221 actactaaat aattatctaa tatgcgctta ggctgccagt aaatgaatcc cgttctcatg
    11281 ctcaggttgg gattagcaag cagcggatgg cagaataaaa cttgacaaaa caggctcgac
    11341 aatcgcaagg gtttagagcg atttcctcag tttcgtatgg ccaggggcta actcactggc
    11401 ctgtagttga tagctggaaa ccccataagg acaaggagcc ccccatgcag tatccaacca
    11461 cccgcaaaga cgatatcaaa gaggagtctt tcggcactct ggtccaagat ccctaccgct
    11521 ggctggaaga cgatcacagc gacgaaacca tcgcctggct gaaagcccag caggagctca
    11581 cggagtctta cttaaactca tttccaggcc gcaaggcgat gctggagcgc gtcagagcgc
    11641 tgaacgacta tcccaaacag acctgcccca caaagcacgg cgactggtat taccactaca
    11701 aaaacgatgg tctgcaaaac cagtgggtaa tgtggcgccg caaagctggc gaggagacgg
    11761 aagagctgtt tttcgaccca aatactatct cagaggacgg gaccacaagg gcttatccca
    11821 tcggctggtc caaggattac cgctatgtct gcttcaacta ttcccaggcg ggagccgatc
    11881 tgggagaatt caggatcatg gacctggcca caaaagagtt tctgccagac gtcctgaagg
    11941 atatgcgcca caccaacgtt gcctggtata aggacggctt tttctatggg cgctatgata
    12001 aaagccagga ttatcaggct caggactgcg atcaaaagat atatttccat cgtctgggtg
    12061 atcccgtttc cgaggacaaa cttgtctttg aagatcctga gcacccgctc cgctttcttc
    12121 acacctacgt ttccgacgac gaaaagaccc tctttctcta ccaatctcag ggcacgcatg
    12181 gcaatcaggt gcacttccgt cccgctggtg atgacaaggc cccctggacg ctcctgtttg
    12241 agggctttga gtatgatggc tggccgaccg atggttttga agaggggatc ttctatctct
    12301 ttaccaacaa aggggccaag aacttccgtc tgctgaaagt cgatctgacc aaacctcagg
    12361 aagataactg gatcgagatc attcctgagc gggattatcc cctcgattca gtcattcccg
    12421 tgggcggaaa gctgatcgcc atctatacaa aggacgtaca aagccagatc gaggtgctgg
    12481 acgcggatgg caagttcctc tatcccatcc agatgcctta tcagggcgga actcagttta
    12541 cgtgtggacg aagcacagac cgggaggggt atttctattt tgagtctttc ataaagccca
    12601 gtgagagcta ccattatgat gtcgatacca ataccctgag cttttattat cgtgatccgg
    12661 tcccggctga gctggatgcg ctggtctcga agcagatctt cttcagcagt aaagacggaa
    12721 cccgggtgcc gatgaccctt atctataagg aagggttgca gttggatggc tctcatccgc
    12781 tcttgctcta cggctatgga ggctttaaca tatcgctgca accgggattt agtgccagtc
    12841 ggattgccct gatagaacaa ggcttcattt acgtggtcgt aaacctcaga gggggcggcg
    12901 agtatggaga aaactggcac gaacagggga tgctcctgaa caagcagaac gtgtttgacg
    12961 acttctgcgc ggccgcggaa tatctcatca gagagggata tacgtctgcc gacagactgg
    13021 cgatcaatgg tggttccaac ggaggattgc tggtgggggc ctgcctcact cagagaccgg
    13081 agctgttcag ggctgccgtg ccttctatgg gagtgctgga tatgctgcga ttccataagt
    13141 ttacctgcgg ctggggatgg atggtggagt atggcgatcc gggccaggaa gagcagtttc
    13201 gcaaccttct cagctactca cccctgcata atatcaagcc gggagtgaag tatcccgcca
    13261 ccctggtcac caccgccgat cacgacgacc gcgtgatccc aggccactct ttcaagtttg
    13321 ccgccactct tcaggaacat gccgatccgg ccagaccggt gctgctctac acccagtttt
    13381 ccagctccca cggccccagc aatctaaaca agactctgga gctctgggcc gatatctgga
    13441 gctttctgat gctggagcta aagcggtaag cgtgaagcgt tacgcgttac gcgttacgcg
    13501 ttacgcgagg cccgcagcgc agatctctca cctaccgcag gtatctcacc taccgcaggt
    13561 atctcaccta cgcagtatct
//
LOCUS       DAOSFN010000061        13481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3806_length_13481_cov_8.454119, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000061 DAOSFN010000000
VERSION     DAOSFN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13481
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3806_length_13481_cov_8.454119"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1268)
                     /locus_tag="PLO35_05290"
     CDS             complement(393..1268)
                     /locus_tag="PLO35_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH79115.1"
                     /translation="MDKVKALLILGGGSALGLAHIGVISVIEEHYEIAGVVGTSVGAI
                     VGAMTCLGFSSAELLRIASNDLAPLKIFSPLSLDSRISGIFNGKPVVKLFESWTSAGL
                     IEDSLIPYLAVSFDLHTGRSILIDKGRFCDAMRASSSLPVIFTPYELGKHLCIDGGVE
                     HPLPLAFANRIPADVVIAVNVLPKTEPKPLMADPYAEAGKPERFMRYDVLLRSLHHNQ
                     SFLALQAIARYEPDIVINAGKPNGKPFDFHKAEDFYRYGRQQAIKTLQEYGKPDFITL
                     IRRRYKNLLARYGKD"
     gene            1381..4296
                     /gene="uvrA"
                     /locus_tag="PLO35_05295"
     CDS             1381..4296
                     /gene="uvrA"
                     /locus_tag="PLO35_05295"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOH79116.1"
                     /translation="MKTKIIIKGASEHNLKNISLEIPRNELVVFTGVSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGQMEKPKVDYIEGLSPAISIEQKAASKNPRSTVGTV
                     TEIYDYLRVLYARVGEQHCHVCGDKVGSQTVDQMIEHLMQLPAGSKIQIMAPLVQNRK
                     GEHKEELEELRHQGFVRVMINGVVRNLEEEIVLDKKSKHSIDVIVDRVVIKAGVESRL
                     TDSLELALKLTEGLVKIQDPDSGEEQVLSALNACPRCNIGFEELSPQHFSFNSPIGAC
                     PVCNGLGYKLEFDPDLVVPDPQRSIMDGAVAAWGRLEDKKDSWTLTAVQNLARAYNFS
                     LTQPWYQLPDLVRELILFGSKGRKFKVAWQNSRGSGEFQMKHEGVINQLKRRMHETTS
                     EDMRRYYMQFISDKPCDECGGRKLKASSLAVLVGGKDIGVATSMGVREAYQFFSKLKL
                     KGNQLLIASEILKEITARLGFLESVGLHYLTLDRRSPSLSGGESQRIRLASQIGSQLV
                     GVMYILDEPSIGLHQRDNAKLVEMLCRLRDLGNSVIVVEHDEETMRSANMIVDFGPRA
                     GVFGGEIVTTGTLDQIMASKVSLTGAYLSGRLCIPLPPRRVKPDSRLIKISGASHNNL
                     KNIDVDIPIGLMVCVTGVSGSGKSSLINQTLSPHLHNHFFRSTHKVGKHASISGLEHI
                     DKVISIDQQPIGRTPRSNPSTYIKLFDPIRNLFAELPASKMRGYKAGRFSFNVKGGRC
                     EACEGAGVKQIEMHFMADIFVTCEVCKGKRYNNETLSIRYKGKNIADVLDMDVQEACE
                     FFESIPSLMQKLRLLKEVGLDYIKLGQSSTTLSGGEAQRIKLSKELSRASTGNTLYLL
                     DEPTTGLHFDDINKLLKVLQKLVAMGNTVVVIEHNLDVIKCADWVIDLGPEGGDQGGR
                     VVVQGTPEEVAHCDQSYTGKHLREYLQRQRPSCEDPNLEEVSEPKARRGRPSKAKPA"
     gene            4305..6488
                     /locus_tag="PLO35_05300"
     CDS             4305..6488
                     /locus_tag="PLO35_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH79117.1"
                     /translation="MADIRFTYQALTAGIHETGIAGDFTSWEILEMKDLGGVHVITLP
                     VETGKYRYKLIVDGVWMPDPSNPLREPDPFGGENSILIVKAPEAINLTWEEVYNDPAL
                     LGERIEHYIDIVRTGPESYDIRINWYPGLQCSVELLIDGLAMPCHRLGVVGNREVYHS
                     IVRHEGKTFSLAARFSQRYQELYYGSFGFVSTPGDLVPHQVAAQELEIFDVPDWVAGS
                     VIYQIFPDRFCNLDPSLNPDFREWYYDNCKTPPPPGEYLPPHREYYHFVEDWKDCNGL
                     SQNPLLPDSKPDWWSFYGGDLPGVISKLDYLRELGVNLIYFNPLWTAKSNHKYDSADY
                     RSIDPHFGSEAIMKELCSKAKELGIRIILDVAFNHTGETFWAFRDCVEKGEDSPWWNW
                     YDWKKWPLPDPLPEDFDPKEYYQCWWGIKDMPDLNYDLSRPHPAENYIRDIRKAVPNH
                     ALVEYLLDCVSWWLVDIGIDGFRLDVPDEVPYWFWELFRKHVKAIKPDAWLVGEIWHN
                     AQGWVDRRYFDSVMNYAYFKNPLLDYFILKISAMSEFKTRIEEGLARYPFHACAAMMN
                     LLGSHDTWRIASLAKDKLPRLKLAIVFQMCFVGAPHVYYGDEILMEGSRDPDNRRPFN
                     WDWQSDPRAVEHRDLIRELVSLRKQHNLLQTGEFAWLDASEDVIAFSRYSHQGKLGIW
                     INLSDKEYSIPQDCCLEPLFRFGNVSQKRSGLILSAHAAVICPIP"
     gene            6722..7942
                     /gene="coaBC"
                     /locus_tag="PLO35_05305"
     CDS             6722..7942
                     /gene="coaBC"
                     /locus_tag="PLO35_05305"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOH79118.1"
                     /translation="MSKILLCVSGGIAAYKAIDLASRLQKTGYEIRTVLTQNALRFVS
                     ALNFSAITGGSVHSSLFDDADPIPHIHLADWADLIVIAPATANLIAKAAHGLADDLCS
                     TILLAHTKPRLWIPAMNVNMYSHPATEANLATLKARGDHVLEPVTGLLACGYAGKGKY
                     PPNEEVEAAINCYLKYGKDLEGIRVLLTAGAGEEPIDPMRKLTNNSSGKMGIALARAL
                     ALRGAKVRLVHGRISVSVPYYLEEAVEANDAHTMLEAVMSRRQDADWIIKCAAVSDFT
                     PLNKELSKIPKQTSLQLELISTPDILAMLGTMKAPGQKLIGFAAQTEDLIKNAKAKLK
                     KKRLDMICANLLKTAGQDSTEIVVIKANHSNKNAYAHLSGTKDEVAHAIIDHIKSLDI
                     EKHDRDMELDTHDG"
     gene            7932..8684
                     /locus_tag="PLO35_05310"
     CDS             7932..8684
                     /locus_tag="PLO35_05310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH79119.1"
                     /translation="MTDSGYLIIDGANGQLGSYLASYPQLQVYPQLLLYHRRKDRLAG
                     WHDTPERLIRSCDLLDPGALEAVISEANDHFGAYPSRLIHTAAIRSSDAQSVADCDPE
                     IFYNTMRVNLMSAMHMMKAVLKYMPARGFGRMVFLGSSVTATGLSRGAAYAASKAAIV
                     NLVRSAALENAEHGILINCLSPAPVETELSDDYSGDYLEFRKRYFADHIARTPTHKLV
                     SLQELAVLSVLLLDNALQNLTGQEIYVNGGSL"
     gene            8681..9439
                     /locus_tag="PLO35_05315"
     CDS             8681..9439
                     /locus_tag="PLO35_05315"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOH79120.1"
                     /translation="MKRFYLSMIVMLLLLPLGSNVAFRDGEKLVFTVKYGMVHAAEAI
                     LSVKSGTYSGQPVWLLSTDARTYPFFDAFFKVRDKVESWWDKDTLLPHKFVKNLQEGS
                     YKQRRIHTFNHAKGTTTYQKWSFKNSKFATEDMKMLSKTQDVLSAFYYVRNQKLTPGQ
                     KINLNITSDGRSMNTDVLVHKREKVSTIFGSINCLVIEPKLKSGAVFKQSGRIQIWVT
                     DDQYKIPVKLESAVSFGSFVAVLKEAYNVPYKVK"
     gene            9436..>9826
                     /locus_tag="PLO35_05320"
     CDS             9436..>9826
                     /locus_tag="PLO35_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOH79121.1"
                     /translation="MTDLLALSTYDYELPEELIAQYPLQQRSSSRLMRVSRHNGRIEH
                     LSFDRIVDLLNPGEVLVLNDSKVIPARLYGLKENGTRVEVLLLNELEPLVWKCMVHPG
                     KRLKQAQWLSFSPLLRAYVSAADEDGLR"
     assembly_gap    9827..9926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9927..10565
                     /gene="queA"
                     /locus_tag="PLO35_05325"
     CDS             <9927..10565
                     /gene="queA"
                     /locus_tag="PLO35_05325"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOH79122.1"
                     /translation="LQSALDAFWHELEAVGHVPLPPYIKREDELPDRQSYQTVYARER
                     GSVAAPTAGLHFTSALLAALEAKGVRIVKLTLHVGMGTFLPVKQDRIDLHKMHSEFCT
                     VSPDAAKTVNEAKASGKRVIAVGSTSARTLESFFTGSELLSGSRWTDIFIYPGKEMRV
                     VDALLTNFHLPKSSLLMMISAFAGIELIKNAYREAVLERYRFFSYGDAMLIE"
     gene            10570..11274
                     /locus_tag="PLO35_05330"
     CDS             10570..11274
                     /locus_tag="PLO35_05330"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOH79123.1"
                     /translation="MKANYLFDTVESTMLEYEKIRGISSEYANITVRAVSQSGGMGRG
                     GHRWHSPQGGLWFTFDFAHQEAVESFALYAGHCLHKLLRRLYRLDDLKIKWTNDIYYQ
                     DKKIAGILCRYQIVEHFYVIGLGLNTNNTVDPDIIPTQAVPLREILSYDVSNEYLMKL
                     FIAEMNDSKHLLDQPSSYLEYCDANLYGKGRVATLEQGRDLIEGRILGLDEYGHLRLD
                     CGELKTISYGTLVNIG"
     gene            complement(11534..12982)
                     /gene="malQ"
                     /locus_tag="PLO35_05335"
     CDS             complement(11534..12982)
                     /gene="malQ"
                     /locus_tag="PLO35_05335"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOH79124.1"
                     /translation="MKRKGVLLHFSSLPGDYGIGDLGPAATSFAGFLADQGYNIWQVL
                     PLNHRGYGNSPYSPISAFALDPLLASPELLYTRGLIEAEDLETARLPGSDRVAFEQVL
                     RRKGRLLEIAAAAYLKRHDIAAFIHDQAAWIKPYIAFLTLDRLYGDSHWSFWQPQHRS
                     YSETLWQELLRGFEPFMMSQAAVQAILRDQLTELRQALRQLNIELWGDLPIYLAYHSA
                     EVWAHPELFQMDQAGRRLQVAGVPPDAFCEDGQLWGNPIYRWDEQRSEVFELFENRIS
                     DALGYLDRLRLDHFIGYVNYWSVPCPPAGQDNEPKMPDHARDGAWIPAPGEQLFERLT
                     QRFTAYPFIAEDLGILTEEVCRVRERFGFPGMIVLQFCWQHPDPKVAEYPSDRIIYPG
                     THDNLTSRQWFEELDPEAGEYRNFVSYARNRLNWPEAPTASNAASLMMEIALGSGCET
                     CIFPMQDLLGLGANARMNIPGTPLGNWEWRMK"
     gene            complement(12979..>13481)
                     /locus_tag="PLO35_05340"
     CDS             complement(12979..>13481)
                     /locus_tag="PLO35_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79125.1"
                     /translation="SKVELDFANAANELQKLCLLTGNQRIGVKEVERGLGTSRVGTLA
                     DFYRALGRRDLKATLITVQRMLDSEWEALQVLFQFNKFFAVIHKVLALRKNHVSLAEI
                     SAKHLNDLYMDQRQIFLEGSANYSLDTLRTINAILLETDSALKLSLAPNLTLLELCAF
                     RIMAGK"
BASE COUNT         3254 a         3585 c         3591 g         2951 t
ORIGIN      
        1 tacacatcca tctctttatg tctgagcgct ttggacttca agcagcgcat cggagcgagt
       61 gattccccct tcggtgggct ggttgaagtc caagcggccc gcaaactcgc tgccatatat
      121 agcattatcg tggccgcttg aactccaacg gggatcggcc cgcaaactcg ttgtcacgca
      181 tagttttatc gtggccgctt gaactccaac ggggtcactt caaacctctg caccaacggg
      241 gaggaggcca tcccacaagc gctgatcgcg ccttagcaaa cgaggacctt tgctgttcca
      301 caagcatgcg cgagaatcgc ataccgcgta acgcataccg catcacgcat actgggtaac
      361 ccgtatatat atagagggtt gattatcggg aatcagtctt ttccatagcg tgccagcagg
      421 ttcttatagc gtctgcggat cagggtgata aaatccggct tgccgtattc ctggagggtt
      481 ttgatggcct gctggcggcc atagcggtag aaatcctccg ctttgtgaaa gtcgaaaggt
      541 ttgccgtttg gtttgccggc gttgatcacg atatcgggtt cgtatctggc aatggcctgc
      601 agggccagaa aggattgatt gtgatgcaga gaccgcagta aaacgtcata ccgcatgaac
      661 ctttcaggtt ttccagcttc ggcataggga tcggccatga gcggtttggg ctccgtcttg
      721 ggcagcacat tcaccgcgat caccacatcg gccgggatgc ggtttgcgaa agccagcggt
      781 aaaggatgtt ccactcctcc gtcgatgcaa agatgtttgc ccagctcata gggtgtgaag
      841 atcacgggaa gcgaggatga ggcgcgcatg gcgtcgcaga atctgccttt atcgatcagg
      901 atgcttctgc cggtgtgaag atcgaaggag acagcgagat aggggatcag cgaatcctcg
      961 atcaggcccg cggaagtcca gctttcaaac agctttacca ctggtttgcc gttgaatatt
     1021 ccactgatgc ggctgtccag actgagaggt gaaaaaatct tgagcggggc cagatcattg
     1081 gaagcgatcc tcagcaactc cgcggagctg aaccccaggc aggtcatagc tcccacgatg
     1141 gcccccacgc tggttcccac cactccggcg atttcgtaat gctcttcaat cacggagatg
     1201 acgccaatgt gggccagccc caaagcagag ccgccgccca gtattaacaa tgcttttact
     1261 ttatccatat caagtaccgc gcgcatgttg aaaggcgcgc gtttgatgtc aagtgaaaag
     1321 tgtttttcgg ttgacaacaa gcatgtccgc agaaattggt tcgccatacg aggaaaaata
     1381 gtgaaaacaa agatcatcat caaaggcgct tccgagcaca atctcaaaaa catcagtctg
     1441 gaaattcccc gcaacgaact ggtggtattc acaggcgtga gcggatcagg aaagtcttcg
     1501 ctggctttcg ataccctcta cgcggaaggg caacggcgct atgtggaatc gctttccgct
     1561 tacgcgcgtc agtttctggg ccagatggaa aagcctaagg tggactacat cgaagggctt
     1621 tcacccgcca tctcgattga gcagaaagcc gccagcaaga atccccgctc cacggtgggc
     1681 accgttacag agatatatga ctatctgaga gtgctttatg ccagagtggg cgagcagcac
     1741 tgccacgtct gcggtgacaa agtcggctct cagacggtgg accagatgat cgagcatctc
     1801 atgcagttac ccgccggttc caagattcag atcatggccc ctctggtcca aaaccgcaaa
     1861 ggagagcaca aagaagagct ggaagagcta cgccatcaag gctttgtgag agtgatgatc
     1921 aacggggttg tccgcaacct cgaagaggag atcgtcctcg acaagaaaag caagcactcc
     1981 atcgatgtga tcgtcgatcg cgtcgtgatc aaagcgggag tggaatcacg gctcaccgat
     2041 tcattggaac tggccctcaa gctcacggaa ggcctggtga aaatccagga tcccgacagc
     2101 ggcgaagagc aggtcctctc tgccctaaac gcctgtccgc gttgcaatat cggatttgag
     2161 gagctttcgc cccagcattt ctctttcaac agcccgatcg gggcctgtcc agtgtgcaac
     2221 ggcctgggct acaagctgga attcgacccc gacctcgtag ttccggatcc ccagcgcagc
     2281 atcatggatg gtgccgtggc ggcctgggga cgtcttgagg ataaaaagga cagctggacg
     2341 ctcacggccg tccaaaacct cgcgagagct tacaactttt ctctcacgca gccctggtat
     2401 cagcttcccg atctcgtcag agagttgatt ttattcggct ccaaaggcag gaagttcaaa
     2461 gtcgcctggc aaaactcccg tggctccggt gagtttcaga tgaagcacga aggcgtgatc
     2521 aatcagctta agcgtcgcat gcatgaaacc acctcggaag acatgcggcg atattatatg
     2581 cagtttatca gcgacaaacc ctgcgatgaa tgcgggggcc gcaagctcaa agccagttct
     2641 ctggcagtct tggtgggtgg aaaagacatc ggagtggcca cctcgatggg agtcagggaa
     2701 gcctatcagt ttttctccaa actcaagctc aaaggcaatc agctcctgat cgcatccgaa
     2761 atcctcaaag agatcaccgc ccggctggga ttcctggaaa gcgttgggct gcattatctt
     2821 acgctggaca gacgttctcc ctcgctttcc ggaggagaaa gtcagcgcat caggctggcc
     2881 agtcagatcg gctctcaatt ggtgggcgtg atgtatatcc tggacgagcc cagcattggt
     2941 ctgcatcaac gcgacaacgc caagctggtg gagatgcttt gccggctgcg agacttgggc
     3001 aattccgtga tcgtggttga gcacgatgag gaaaccatgc gtagcgccaa tatgatcgtg
     3061 gatttcggtc ccagagcggg agtctttggg ggagagatag ttacgacggg cactctggac
     3121 cagatcatgg ccagcaaggt ctctttgaca ggggcgtatc ttagcggccg gctctgcatt
     3181 ccactacccc ccaggagagt aaaaccggat agccggctga tcaaaatcag cggggccagc
     3241 cacaacaacc tcaagaacat cgacgtggat attcccatcg gtctgatggt atgcgtgacc
     3301 ggggtttcgg gaagcggtaa gagctcgctg atcaatcaaa ccctctcgcc ccatctgcac
     3361 aatcactttt tccgcagcac ccacaaagtg ggtaaacatg cttccatcag cggattggaa
     3421 cacatagaca aggtgatctc catcgatcag caacccatcg gccgcacccc tcgctccaat
     3481 cccagcacct atatcaaact gtttgacccc atccgcaatc tctttgccga actgcctgcc
     3541 tccaaaatgc gtggttacaa ggcgggccgc ttttccttca acgtcaaagg cggacgctgt
     3601 gaagcctgcg aaggagcggg cgttaagcag atcgagatgc actttatggc cgatatcttc
     3661 gtgacctgcg aagtctgcaa aggcaagcgc tacaacaacg aaactctctc tatccgctat
     3721 aaaggcaaga acatcgcgga cgttctggat atggacgtac aggaagcctg cgagtttttt
     3781 gagagcatcc cgtccttgat gcaaaaactg agactgctga aagaagtagg cctggactat
     3841 atcaaactgg gacaatcatc taccacttta tctggagggg aggcgcagag gataaagctc
     3901 tccaaagaac ttagcagagc cagcacggga aacaccttgt accttctgga tgaacctacc
     3961 accggcctgc attttgacga catcaacaag ctgctcaaag tgctgcaaaa gcttgtggcc
     4021 atgggaaata ccgtggtggt gatagaacac aatctggacg tgatcaaatg cgccgattgg
     4081 gtgatcgacc tcggccccga aggtggagac cagggcggca gagttgtggt gcagggcact
     4141 ccggaagagg tggcacactg cgatcaatcc tacaccggca agcacctgag ggagtatctc
     4201 caacgccagc gacccagctg cgaagacccc aacctggaag aggtttcaga gcccaaagcc
     4261 cgcagaggca gaccttccaa agctaaacca gcctaaggaa gccagtggcc gatattcgtt
     4321 ttacttatca agcccttaca gccggcatcc atgaaaccgg catcgcggga gacttcactt
     4381 cctgggagat cctggagatg aaagacctgg ggggtgtgca cgtgatcact ctgccggtgg
     4441 aaacaggcaa gtatcgctac aaactgattg tggacggcgt ctggatgccc gatccctcca
     4501 atccgcttcg ggaaccggat ccctttgggg gcgagaattc gatcctgatc gtcaaggctc
     4561 ctgaggcaat aaacctcaca tgggaagagg tatataacga tcccgccttg ctgggcgagc
     4621 gcatcgaaca ctatatcgac atcgtcagaa ccggccccga gagctatgat atcaggatca
     4681 attggtatcc gggtttgcaa tgctccgtgg aactgctcat agacggtctg gcgatgccat
     4741 gtcaccggct gggcgtggtg ggaaaccggg aagtttatca cagcattgtg cgacacgaag
     4801 gtaagacttt cagcctggcg gcccgtttct cgcaacgcta tcaggagctt tactatggca
     4861 gttttggctt tgtaagcacg ccgggagacc tggtgcccca tcaggttgct gctcaggaac
     4921 ttgagatctt tgatgtgccg gattgggtgg caggcagcgt gatctatcag atcttcccag
     4981 atcgcttttg caatctcgat cccagcctca atcctgattt tcgcgagtgg tattatgaca
     5041 actgcaaaac tcctcctccc ccgggtgagt atctcccccc gcaccgggaa tactaccatt
     5101 ttgtggaaga ctggaaagac tgcaacggtc tcagccagaa tcccctgctc ccggattcaa
     5161 aacccgactg gtggagtttt tatgggggag accttccggg cgtgatctcg aagctggact
     5221 atctacggga attgggagtc aacctcatat atttcaatcc cttgtggacc gctaaatcaa
     5281 atcataaata cgactcggcg gattacagga gcatcgatcc ccactttggg agcgaagcca
     5341 tcatgaaaga actttgctcc aaagctaagg aactggggat cagaatcatc ctcgatgtgg
     5401 ctttcaacca caccggcgag accttttggg cttttaggga ctgcgtggaa aagggagaag
     5461 actcaccctg gtggaactgg tacgactgga aaaagtggcc gctgccggat cccctgcccg
     5521 aggattttga ccccaaagag tattatcaat gctggtgggg tataaaagac atgccagatc
     5581 tcaattatga cctcagtaga ccccatcccg ccgaaaacta catccgcgat atccgcaagg
     5641 ccgttcccaa tcacgctctg gtggaatatc tgctggattg cgtgagctgg tggctggttg
     5701 atattggcat cgacggcttc cggctcgacg tcccggatga ggttccctac tggttttggg
     5761 agctgtttag aaagcacgtg aaggctatca aaccagacgc ctggctggta ggggagatct
     5821 ggcacaacgc ccagggctgg gtagaccgcc gttacttcga ttccgtgatg aactacgcct
     5881 atttcaaaaa ccccctcctc gactacttca tcctcaaaat cagcgccatg agtgagttca
     5941 aaaccagaat tgaagagggg ctggcgaggt atcccttcca tgcctgcgcg gcaatgatga
     6001 accttctggg cagccacgat acctggagga tcgcgtctct ggccaaggat aaactcccgc
     6061 gtctcaagct ggccatcgtg tttcaaatgt gctttgtggg cgctcctcac gtctattacg
     6121 gtgatgaaat cctcatggag ggtagccgcg atccggataa ccgcagacct ttcaattggg
     6181 actggcaatc agacccccgg gcagtggagc atcgcgatct gatcagagag cttgtcagcc
     6241 tcagaaagca gcacaatctg ctccagacgg gagaattcgc ctggctggac gcctctgagg
     6301 atgtgatcgc cttttctcgt tattcccatc aaggcaagct tggcatctgg atcaatctct
     6361 ctgataagga atattcaatc ccgcaggatt gttgcctgga acccctgttc cgctttggta
     6421 atgtgagcca gaagcgctcc ggtctgattc tctcagccca cgcggcagtg atatgtccaa
     6481 tcccctgaac tgccatttac accatccgga accaggcctt gggggctgtt tgctgtccgt
     6541 ctatggtgca ggttccctgc ctgatacctg ccgtggagat gtggtgctcc cgggggcgaa
     6601 aaacggcgga gagcgcacgg catctctacc gcgactgaca agcaaggaag ctctatgacg
     6661 aatctgattg acattttcaa tgcctttgcc agattggata aaactgagaa gaggcatgtt
     6721 gatgagtaag atactacttt gcgtgagtgg cggtatagcc gcttacaaag ccatagatct
     6781 ggccagccgg ctccaaaaga ccggctatga gatcaggacc gtgctcacac aaaacgctct
     6841 gcgctttgtt tcggcgttga atttcagcgc catcacgggc ggaagcgtcc attcttcttt
     6901 gtttgacgac gctgacccca tcccgcatat tcatctggcg gactgggcgg atttgatcgt
     6961 gatcgctccc gccacagcaa acctgatcgc caaagctgcg catggactcg cggacgatct
     7021 ctgctccacc attctcctgg ctcatacaaa gccccggctc tggattcccg ccatgaacgt
     7081 caacatgtat tcccatcctg ccaccgaggc taatctcgcc accctcaaag cgcggggaga
     7141 tcatgtgctg gaaccagtta cgggattact cgcttgcggc tatgcaggca agggaaagta
     7201 tcctcccaac gaggaagtcg aggctgccat caactgctat ctgaagtatg gcaaagactt
     7261 ggaaggcatc agagtactgc tgacggccgg ggctggtgaa gaacctattg atcccatgcg
     7321 caagctcacc aacaactcca gcggcaagat gggcatcgcc ctcgccagag ctttagcgct
     7381 acggggcgcg aaagtgcgcc ttgtgcatgg aaggatcagc gtttcagtac cttactatct
     7441 ggaagaggct gtggaggcca atgacgcgca cactatgctg gaagcagtga tgagcagacg
     7501 ccaggatgcc gattggatca tcaaatgcgc cgcagtgtct gattttacac ctctcaataa
     7561 agagctaagc aagatcccca aacagacatc cctgcagctg gaattgatct caactccaga
     7621 catacttgcc atgttgggca ctatgaaagc tcccggacaa aagctgatcg gctttgccgc
     7681 ccaaactgag gacctaatca aaaacgccaa agcaaaactc aagaaaaagc ggctcgatat
     7741 gatctgtgcc aacctgctca aaacggctgg tcaggacagc acggagatcg ttgtgatcaa
     7801 ggcaaaccat tccaataaaa atgcttatgc gcacttatcc ggtaccaagg atgaggtggc
     7861 acatgctatc atagaccaca tcaagagctt agacattgaa aagcatgaca gagatatgga
     7921 gttggatacc catgacggat agcggttacc tgatcatcga cggcgctaac ggtcagctgg
     7981 gatcatacct ggcaagctat cctcagcttc aggtctatcc acaattgctg ctttaccata
     8041 gacgcaagga cagattggca ggatggcacg atactcctga gcggttgatc aggtcttgcg
     8101 atctgctgga cccgggtgct ctggaagcag tcatctccga ggccaatgat cactttggag
     8161 cttatccctc caggcttatt cacacggccg ccattcgcag cagcgatgct caaagcgtgg
     8221 cggactgcga cccggagatc ttttataaca caatgagggt caatctgatg agcgccatgc
     8281 atatgatgaa ggcggtgctc aaatatatgc cggccagggg attcggcagg atggtgtttt
     8341 tgggctcttc cgtcacggcc acaggtctgt cgcggggggc agcgtatgct gcttccaaag
     8401 cggcaatcgt caatctggtc agaagcgccg ccctggaaaa cgcggagcac ggcatcctga
     8461 tcaactgtct ttccccggct cctgtagaga cagagctaag tgacgattac agcggcgact
     8521 atcttgagtt tagaaaacgg tattttgccg accatattgc cagaactccc acccacaaac
     8581 tggttagttt gcaggaattg gcggttttga gtgtattatt gttagacaac gccctgcaaa
     8641 atctgacggg gcaggaaatc tatgtgaatg gaggttcgct gtgaagcgct tttacctgtc
     8701 tatgatcgtg atgctgctgc tccttcccct gggctcgaat gtggctttca gggatggaga
     8761 gaaactggtc tttaccgtca aatacggtat ggtccatgcc gctgaggcga ttttgagtgt
     8821 taaatccggc acctactctg gccagccggt atggctgttg agcaccgatg cccgcaccta
     8881 tccctttttt gacgctttct tcaaagtgcg ggacaaggtg gaatcctggt gggacaaaga
     8941 tacgctgctc ccccacaagt ttgttaaaaa cctgcaggaa ggcagttata aacaacgccg
     9001 catccacacc ttcaaccacg ccaaaggtac taccacctac cagaaatgga gctttaaaaa
     9061 ctccaagttt gccaccgagg acatgaagat gctcagcaaa acccaggatg tgttgagtgc
     9121 cttctattac gtgcgcaatc aaaagctcac acccggacaa aagattaatc taaacataac
     9181 ctccgatggc agatccatga atacggacgt gttggtgcac aagcgtgaaa aagtcagcac
     9241 catctttgga agcatcaatt gcctggtgat cgagcccaag ctgaagagcg gagctgtatt
     9301 caagcagagc ggccgcatcc agatctgggt gacggacgac cagtataaaa tccccgtcaa
     9361 gctggaaagc gccgtttctt ttggctcttt tgtggcagtt ttgaaggaag cctacaacgt
     9421 accctacaag gtgaaatgac cgatcttctg gccctgtcaa cctatgacta tgagctccct
     9481 gaagagctga tcgcccaata ccctctccag caacgaagca gctcacgttt gatgagggtt
     9541 tccaggcata acgggcgcat cgaacacctg agctttgaca ggatcgtgga cctgctgaat
     9601 cccggggaag tattggtgct aaacgacagc aaggtaattc ctgctcgcct gtatggcctc
     9661 aaagaaaacg gcacccgggt ggaagtcctg ctcctgaacg aactggaacc gctggtctgg
     9721 aagtgcatgg tccatcccgg caaacgtttg aaacaagccc aatggctgag tttctctcct
     9781 ctgctccggg cttatgtgtc tgcagccgat gaggatggcc tgaggcnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnncttc aaagcgccct tgacgctttc tggcatgagc
     9961 tggaagcggt gggccacgtt cccctgcctc cctacatcaa acgtgaagat gagcttcccg
    10021 accgccagtc ctaccagacc gtctatgccc gtgaacgtgg atcggtggcg gccccaactg
    10081 ccggactgca cttcacgtcc gcgctactgg ctgctctgga agccaagggg gtccggattg
    10141 tcaagctcac tctgcatgtg gggatgggta cctttctgcc ggtcaagcaa gacaggatcg
    10201 atctccataa gatgcacagc gagttttgca ccgtgagtcc agatgccgca aagactgtaa
    10261 atgaagccaa agcaagcggc aaacgcgtca tcgcggtggg cagcacatca gcgcgcaccc
    10321 tcgagagctt tttcaccggg tcagagctgc tcagcggatc gcgctggacc gatattttta
    10381 tctaccccgg caaagagatg agggtggtgg atgccctgct caccaacttc catttgccca
    10441 aatcaagctt gttgatgatg atctccgctt tcgcggggat cgaactgata aaaaacgcct
    10501 atcgtgaagc cgtgctggag cgttacaggt ttttcagcta cggggacgcg atgctgatcg
    10561 agtgagttta tgaaagccaa ttatctcttt gacaccgtcg aaagcaccat gctcgagtat
    10621 gaaaagatcc gtgggatcag tagcgaatat gccaatatca ccgtgagggc cgtatctcaa
    10681 tccggtggga tgggacgggg cggtcatcgt tggcactccc ctcagggtgg tttgtggttc
    10741 acctttgatt ttgcccatca ggaagcggtg gagtctttcg ctctgtatgc cggacactgt
    10801 cttcacaagc tgctccgcag gctctaccgc ctggatgatc tcaagatcaa atggacaaac
    10861 gatatctatt atcaggacaa gaagatagcc ggtatcctct gtcgttatca gattgtcgag
    10921 cacttctacg tgatcggtct gggattgaac acaaacaaca ccgtcgatcc tgatatcatt
    10981 cccactcagg ccgtgcccct ccgggagata ttgagctatg atgtatccaa tgaatacctt
    11041 atgaaactct ttatcgcgga gatgaatgac agcaagcacc tgctggatca gccctccagc
    11101 tatctggagt attgcgacgc caatctctat ggaaagggac gcgtagccac tttagaacaa
    11161 ggaagagacc tgatcgaagg cagaatcctg ggtctggacg aatatgggca tctgcggctc
    11221 gactgtggag agctaaagac aatcagctac gggacgctgg tgaatattgg gtgagagctg
    11281 aagcctgtga gatacctggc cacgccacaa agtcactagc cttagaggct cactactcag
    11341 tagtgtctta tcgcctggtc atgggcaagc ccggacaaag tcggaagcag acccctgaaa
    11401 tggcctttgg atcgggtatg ctcgtccgcg ggattacccc catcagcaag aggtccttag
    11461 cagaggaatc gggatgccgt aaaacacaaa gttgtatggc taaccacttt atctgcaaat
    11521 agatggatga tatctatttc attctccatt cccaattgcc aagcggagta ccggggatgt
    11581 tcatcctggc gttggccccc aaacctaata ggtcctgcat agggaaaatg catgtttcgc
    11641 aaccacttcc cagggcgatt tccatcatca gggaggcggc gtttgaggcg gtgggggctt
    11701 ccggccagtt cagtctattg cgtgcgtagc tcacaaagtt gcgatattcg cccgcttccg
    11761 gatcaagctc ctcaaaccat tgacgggaag tgagattgtc gtgggtgcca gggtagatta
    11821 tccggtcgga tggatattcc gccactttgg gatcgggatg ctgccagcaa aattggagca
    11881 cgatcatgcc gggaaagcca aagcgttctc tcacccggca gacttcctcc gtcaggatgc
    11941 caagatcttc cgcgataaaa ggataagccg tgaaacgctg ggtcagccgc tcaaacaact
    12001 gctcaccggg agctgggatc caggcaccgt cgcgagcgtg gtctggcatt ttgggctcat
    12061 tatcctggcc agcaggtgga cagggcacgc tccaatagtt cacatatccg ataaagtggt
    12121 ctaaacgcag gcggtccaga tagcctagtg cgtccgatat cctgttttca aagagctcga
    12181 atacctcaga gcgttgctcg tcccagcggt aaatcggatt tccccagagc tgtccatcct
    12241 cgcaaaaagc atcgggaggc acgcccgcta cctgtaaacg tctgcccgcc tggtccatct
    12301 gaaacagctc cggatgagcc caaacttcag cactgtggta ggccagatag atgggaagat
    12361 cgccccaaag ctcgatatta agctgcctga gagcctgcct cagttccgtg agctggtccc
    12421 tcaaaatagc ctggacagcg gcctgggaca tcatgaacgg ttcaaagccg cgcagcagct
    12481 cctgccacag cgtttcgcta taggaccggt gctgaggctg ccagaaactc caatgcgagt
    12541 ctccatagag cctgtcaagt gtcaaaaaag cgatgtaggg cttgatccag gcggcttgat
    12601 cgtggataaa agccgcaatg tcgtgccttt taagataagc cgccgcggca atttccaaca
    12661 gccttccctt gcgcctgagc acctgctcaa aagccactct gtcactgcct ggcagcctgg
    12721 ccgtttccaa atcttccgcc tcgatcaagc cccgcgtata cagcaattcc gggctggcca
    12781 gcaaaggatc aagcgcgaac gcggaaatgg ggctgtaggg agagtttcca tagcctcgat
    12841 gattgagcgg cagcacttgc cagatgttgt aaccctgatc tgccaggaag cccgcgaatg
    12901 atgtggcagc cggccccaga tcgccaatcc cataatctcc gggaagggag ctgaaatgca
    12961 gcaggacccc tttgcgcttc attttccagc cattatcctg aacgcgcaga gctccaggag
    13021 cgtgaggttt ggggccagac tcagtttgag cgcgctgtct gtttccagca gaatggcatt
    13081 gatggtcctg agcgtatcca aggagtaatt ggcgctgcct tccagaaata tctggcgctg
    13141 gtccatgtat aaatcgttga gatgcttggc gctaatctcc gcgaggctta catggttctt
    13201 gcgcagggcc agcaccttgt ggatgacggc aaagaattta ttaaactgaa ataatacctg
    13261 cagagcttcc cactcggaat ccagcattct ctgaacggtg atcaaggtgg ctttgaggtc
    13321 tcttctgccc agagcgcggt aaaaatctgc cagggtgccc accctggaag ttcccaagcc
    13381 gcgctctacc tctttcacac cgatgcgctg attgccggta agcagacaca gcttctgcag
    13441 ttcgttggcg gcattggcaa aatccagctc taccttggag a
//
LOCUS       DAOSFN010000062        13338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3879_length_13338_cov_9.012949, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000062 DAOSFN010000000
VERSION     DAOSFN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13338
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3879_length_13338_cov_9.012949"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..679)
                     /locus_tag="PLO35_05345"
     CDS             complement(179..679)
                     /locus_tag="PLO35_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOH79126.1"
                     /translation="MLDIAILNKIPLFKDLRDDQLSRLQPFLKKVEYPKGSYILRENT
                     TGDNIFLLVEGVAVVTKDLVMGFDEDRASTEKVLHTMRSEHLPTFGENGILGHAPRTA
                     NIIAQTDCVLYTLSKEDFDSYAAEDYQAGYYLVLNIARVISQRLSNTDINLVKLATAL
                     YIAVRQ"
     gene            complement(691..1875)
                     /locus_tag="PLO35_05350"
     CDS             complement(691..1875)
                     /locus_tag="PLO35_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOH79127.1"
                     /translation="MKTQRSGLKKLRQAILRKIPVAADHRRLAFFFTLVCLIIVALLL
                     ACALLVWVFEAQDPAHRNITSFWDGIWWAIVTIATVGYGDRFPVTYPGRVVGLVLIVV
                     GYSLLSVFAGLIASLFVEDRMKGAKGLKQIRTQDHIVICGWNNTAEFLLRALLEKQLQ
                     DVEICLLINQPPEFFEAIESRYPNLQLRFVRGEATSEEVLKRASVDKAAQVIILADHN
                     LAAQSADDRSIIVANAMRYLVPKERITIQLNNPENRNLLNRIGITRVMVFDDLGGYML
                     ANNVTEDNSIELFSHMIKNHQTQIITLDIPESFVGKSFEALREHFWKDGRRILLGLMA
                     KEPQLELDDIFADDGSAIDQFIKSTLGKVRAAGQEDKSSVVLKPPPEHLIQVNDLAIV
                     LN"
     gene            complement(1880..2458)
                     /locus_tag="PLO35_05355"
     CDS             complement(1880..2458)
                     /locus_tag="PLO35_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79128.1"
                     /translation="MIFLKSLTFILWNMAIGTIIVLKVDWLLFNLKRRKFLGIHIPFT
                     PGFVVSKREWVFNKARDILHDYLEQAEDKLRKNGYLAKWERLVRDTVYDKTNFVDEWM
                     FLPRKWKDKIHDLIANSVKDIVSKILRRTVPHFVEQWRIEHRIDEFDEQFNAEFLRKY
                     WRKYVLKYMLYAFLAINFLIGITNLIWFLIIA"
     gene            complement(2459..3220)
                     /locus_tag="PLO35_05360"
     CDS             complement(2459..3220)
                     /locus_tag="PLO35_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOH79129.1"
                     /translation="MWPLLLLALLALGLGASEIPAPQGFVNDYARLLSEETRVRLNDW
                     AIELKEKTDVELAIGIFESIGTMDENSHAVKVFETWKIGNQRDEGVLIMLALKERRLK
                     IEVGYGSEPYVTDSYSGDVYRAMRDMLPKGREDWDGAFTQGMLLLLQRIAREKGVTIS
                     GVSEFSKGTNNIRADKAKGVVGLFGVLIFIFLIIVTRGRILEVLLWMWILGGRGGGGS
                     WGGGSSGGGGGFGGFGGFGGFGGGRSGGGGAGGGF"
     gene            complement(3228..4451)
                     /locus_tag="PLO35_05365"
     CDS             complement(3228..4451)
                     /locus_tag="PLO35_05365"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOH79130.1"
                     /translation="MTNLHTENIFASVDEAVKIIAAGGMLIVVDDENRENEGDLVMAA
                     DLVNTEAVNFMISHAKGLLCVPMREEALQRLDIPMMTTSSSDRHGTKFTVSVDAMEGT
                     TTGISASERALTIKGLADPDSQARDFMRPGHIFPLLADKGGVLKRAGHTEAAVDLAVM
                     AGRAPVGAICEIIREDGEMARLDDLVIFARKHALKIFTIAELIRYRRHQECLVERVSV
                     AKLPTRYGLFDIYTYISTISDEYHLALVMGKIDPTQAVLTRVHSECLTGDALHSLRCD
                     CGDQLRRAFEMISEEGRGVILYMRQEGRGIGLLNKIKAYHLQDGGKDTVEANLDLGFP
                     ADLRDYGIGAQILKQLGIKKLRLLTNNPKKIVGLQGYGLEIVERVPIEIEAGAENLDY
                     LKTKKRKMGHILSDV"
     gene            complement(4466..5485)
                     /locus_tag="PLO35_05370"
     CDS             complement(4466..5485)
                     /locus_tag="PLO35_05370"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH79131.1"
                     /translation="MRQRIKIRFEDTQAQRLDKYLSDLRIQELYSRTFIEKLIEEDRV
                     LVNLFPVKKSYLLQAGDEVDISLPEPEPQEIVPQDIPLEVVYEDEHLAIINKEPGLIV
                     HPGFGNPDRTLVNAILFRWGSELSSGRAPDRPGIVHRLDSGTSGLMIIAKNDMAQSKL
                     NDMFAYRDVVKTYLAVSCGIPEEAAGSIETNIARSLTNPRKMCVAAEGRHAVTHYKIV
                     HWFHYFALHKIQLETGRMHQIRVHFAHMGYPLLGDLLYNTRAQVHALVPQNMKRRVTE
                     LLTTHLRRQALHAWRLQFVHPISRKPIDVKAPLPEDLLYALDWLDSNFAIDKPGLDLE
                     AILND"
     gene            complement(5469..6212)
                     /locus_tag="PLO35_05375"
     CDS             complement(5469..6212)
                     /locus_tag="PLO35_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79132.1"
                     /translation="MKRSFRRRYHVLLMILLGIILLQSGLIWFTLSQATDLTDLVSDV
                     QSILIIFFFAVFVYMIVIYNYVPFRLRRAFRDIGNLIEEISHGNYQIDIDSSTYDQDA
                     DIQELIMALQKMLNILMRFDMVKADKIYEHHQRITQMINILPFGILIGSVNGDVVYCN
                     DKLREAYPNITETVNLNELIFTNPFDSRLFHVIGESMRYGKNLYQDIVADDKEDRQAA
                     VDGSIIRNRKGVATGAVFVIHFMDNASAD"
     gene            complement(6216..6920)
                     /locus_tag="PLO35_05380"
     CDS             complement(6216..6920)
                     /locus_tag="PLO35_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOH79133.1"
                     /translation="MARYAVIGLGRFGMTVATILAEYGMDVLAIDKSQELIDEVSGKV
                     ASAVCMDSTDENTLRTLNLNEVDAVIIGIGSNIQECILTAAILKKIGVSVIYAKVENR
                     LHGRIMELIGVQNILLPEEIVGNQLAKKLISTNILEYTNLSSGHTMIELIAPKDFIGK
                     TLQELSLPLTRGINVIAIKYSHLSVTEDGRNVIEKRLNDMPGANDVVNEGDVLILLGP
                     KGNVDKLIYDTSARRD"
     gene            complement(6934..8625)
                     /locus_tag="PLO35_05385"
     CDS             complement(6934..8625)
                     /locus_tag="PLO35_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOH79134.1"
                     /translation="MLKSITRGLLARIELVAYLLAIWSFVVLFMESVISFYADFSIVE
                     HLTALANILLLFLTIINRLITREPGKGNNLLIFDGSMLALGTLLFFFDAKFVIFFLLI
                     RQTWYILEFFLFKAFEGRFFKQLTANPPATLMLSFALVISIGTILLMMPAASSSNQVT
                     NIVDALFTATSATCVTGLTVVDTGSHFSFFGQLVILVLIQIGGLGIMTISTAFAIMLG
                     QRITLKLESVMYDVVGGSQSLNVLQLLKSIVLATLLIESLGAVLLYTRFSAHYLPLEA
                     FYMSVFHSVSAFCNAGFSLMSENMMGYVSDIVMNLGITFLIILGGLGFTVIVDLNRYL
                     FYRERVKKLSLHSKIVLITTGSLLLLGVVTFYWAEYYGTMEGFTIAKRLLASWFQSVT
                     TRTAGFNTIDTSVLSKASILITLALMFIGASPGSTGGGIKTTTFAVLSLSITSLLKGK
                     RELSIFNRKIPSTNFREATSLITLSAAIIFVILFLLLLVEPFPFDKLFFEAVSAFGTV
                     GLSTGITPLLSQTGKVLITLLMYVGRIGPLTMIYALAIKRHVINLDYAEEKIAIG"
     gene            complement(8638..9261)
                     /locus_tag="PLO35_05390"
     CDS             complement(8638..9261)
                     /locus_tag="PLO35_05390"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOH79135.1"
                     /translation="MQCIVIFGIQGSGKGTQAKLLSESLGYQHVNIGDLLRDQVSRGT
                     ELGNEVKAVIARGELVEDELIFRLIANAIQANAKGIVFDGFPRTEAQAEHLGKHFTVR
                     KVFYLDLSEEEAISRIGARRVCQSCGENFNLNSKKPAKEGVCDRCGGKLGIRQDDRPE
                     AIRKRMIEFYAQTQGLTSWFEAHGLLARIDAGKSVEAVFRILMEGVS"
     gene            complement(9316..9690)
                     /locus_tag="PLO35_05395"
     CDS             complement(9316..9690)
                     /locus_tag="PLO35_05395"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOH79136.1"
                     /translation="MIVGIGTDIIKVERIQKSIEANPRFGEKLFTPAETAYCESRASK
                     YQSYAARFAAKEAVMKALGTGWNELVHWLNIEIVREGDGAPKVLLHNETNAFAASLGI
                     QAIHLSIAHEKEYATAFAVLEK"
     gene            complement(9687..10190)
                     /locus_tag="PLO35_05400"
     CDS             complement(9687..10190)
                     /locus_tag="PLO35_05400"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOH79137.1"
                     /translation="MKAKKHSYINQTPSPAERISLYYNQGCFHFKLELEGDLVVRVGF
                     LKPDGPEAAEPNSAHPYIDMLDCYLRGINQRLELPHALRVTDFARKVYTATSDIPPGE
                     TSTYGAIATRIGAPQAARAVGAALRANPLPLIVPCHRVVGASGKLTGFAGGLELKLRL
                     QELEKRS"
     gene            complement(10449..10802)
                     /gene="rplS"
                     /locus_tag="PLO35_05405"
     CDS             complement(10449..10802)
                     /gene="rplS"
                     /locus_tag="PLO35_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOH79138.1"
                     /translation="MDILHTVGRDQIRTDFPEYRVGDTVKVHYKIKEGSKERVQVFQG
                     IVIQKRGAGVSKTFTVRKVSNGIGVERIFPQHSPNIDKLEIVRFGQVRRAKLFYLRSA
                     KGKAGRIKERRRFTA"
     gene            complement(11370..12053)
                     /gene="trmD"
                     /locus_tag="PLO35_05410"
     CDS             complement(11370..12053)
                     /gene="trmD"
                     /locus_tag="PLO35_05410"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015881368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOH79139.1"
                     /translation="MTIDVLSLFPRGFDGFKDVSIVARAIQKGAVKLNFTDFREFSAD
                     KHRKVDDYAYGGFPGLVLQPQPIWDALVHLLEDGFAPVIYFSPQGRPLTQPILEFYAQ
                     LPRVILLCGHYKELDQRVRDLAISDELSLGDFVLSGGEIAAMAFIDGVARLQDGVLTD
                     AASARSDSFSDGKTKLGFPCYTRPDTWMGIEVPAVLKNGDHAKIEAWAEERSQAMTHA
                     RRPELLTKR"
     gene            complement(12060..12578)
                     /locus_tag="PLO35_05415"
     CDS             complement(12060..12578)
                     /locus_tag="PLO35_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79140.1"
                     /translation="MEVSDLIGIGRLGGMDAQGFFQVLIKPRYRPVFEDAKCVFLIFK
                     SDRVFYVTISERKVSDKKIMVRFIEDGIDIERRLHREVLIALEPEFLDEDEGLDHLLG
                     YEVLFEDRSLGTVKDYFHNHAQYVLVIGTPTGKELLVPYVDHYVQAEIPDPGVLLLQS
                     AQPLIDALDSDS"
     gene            complement(12579..12809)
                     /locus_tag="PLO35_05420"
     CDS             complement(12579..12809)
                     /locus_tag="PLO35_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOH79141.1"
                     /translation="MKDLIEFMVKALVDDPSEVNITEIAGDRITLYELRVSKSDIGKV
                     IGKRGRTAGAIRTIINAVSTKQGKRAELEIIE"
     gene            complement(12822..13121)
                     /gene="rpsP"
                     /locus_tag="PLO35_05425"
     CDS             complement(12822..13121)
                     /gene="rpsP"
                     /locus_tag="PLO35_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013507147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOH79142.1"
                     /translation="MMVKLRLRRMGANDQPFYRIVAVDARVKRDGKYIECVGWYDPKP
                     NPSKINIETDRAIYWLSVGAQPSDTVRSLLRKAGVLQIWHERKIAKQAATEIDTQ"
BASE COUNT         3051 a         3508 c         3547 g         3232 t
ORIGIN      
        1 atattatgac cgccatatgt atcaatatgt tacgaaatta aacgcgaaag ttgggttaat
       61 cgcaatcaga gccagggttc agcctaccca atccggactt tgtaagggat gccccggagc
      121 tgccatactc cctgcagaga gtaatgaata agagagaagc gtggctctgc caggcaagct
      181 attgtctgac ggctatatag agcgcggtag ccagcttcac gagattgata tcggtgttgc
      241 tgaggcgttg cgaaatgacg cgcgcgatat taaggaccag gtaatatccc gcctgataat
      301 cctccgccgc gtagctgtca aagtcttctt tggaaagggt atagagcacg cagtcggtct
      361 gcgcgatgat attggcagtg cggggagcgt gccccagaat gccgttttcg ccaaaggtgg
      421 gcaaatgctc gctgcgcatg gtgtgaagca ccttttcggt ggaggcgcgg tcttcatcga
      481 agcccatcac aaggtctttg gtgaccaccg ccacgccctc caccagcagg aagatattgt
      541 ctcctgtagt gttctccctc aggatataac tgcctttggg gtattcaact tttttcagga
      601 aaggctgcag gcgggaaagt tgatcatccc gcaggtcttt gaagagaggg attttattga
      661 ggatggcgat atccaacatg tccggctccc ttaattcagc acgatggcaa ggtcgttcac
      721 ctggataaga tgctctggcg gaggcttgag caccacgctg ctcttgtcct cctgtcccgc
      781 cgcccgcact tttccgaggg tggatttgat gaattgatcg atggcgctgc catcatcggc
      841 aaagatgtca tccaactcca gttgtggctc tttggccatg agacccagca aaatccgccg
      901 tccgtccttc cagaagtgct ctctcagagc ttcaaaactt ttgccaacaa agctttcagg
      961 gatatccagg gtaatgatct gagtctggtg gtttttgatc atgtggctaa agagttcgat
     1021 gctgttatcc tcggtcacgt tgttggccag catatagcct cccaggtcgt cgaagaccat
     1081 cactctggtg ataccgatgc ggttgaggag gttgcggttt tctgggttgt tgagctggat
     1141 ggtgatgcgt tccttgggca ccagatatcg catggcgttg gcaactatga tggaacggtc
     1201 atccgcgctt tgggcggcaa ggttgtggtc cgccaggatg atcacctggg ccgctttatc
     1261 caccgaggcg cgtttgagta cctcttcaga ggtggcctct cccctcacga aacggagttg
     1321 gagattggga tagcgtgatt cgatcgcctc aaaaaactcg ggcggctggt tgatcaggag
     1381 gcagatttcc acgtcctgca attgtttttc cagcaaagcc ctcaacagga attccgcggt
     1441 gttgttccaa ccgcagatca cgatgtggtc ctgggttctg atctgtttca aacctttggc
     1501 tcctttcatt ctatcctcca caaagaggga ggcgatcaaa ccggcaaaca ctgagagcag
     1561 cgaatagcct accacgatga ggacgaggcc taccacgcgg cctggatagg tgacgggaaa
     1621 acgatcgcca taaccgacgg tcgcgatggt gacaattgcc caccagatgc cgtcccagaa
     1681 ggatgtaatg ttgcgatggg cgggatcctg ggcctcgaaa acccacacca gcaacgcgca
     1741 ggccagcagc aaggcgacaa tgatcaggca gacgagagtg aagaagaaag ccagtctgcg
     1801 atggtccgcg gccacgggaa tcttgcgtaa gattgcttgc cgcagcttct tgagaccaga
     1861 gcgctgggtt ttcatggatc tacgcgatga tcaggaacca gataagattg gtgatgccga
     1921 tcaggaagtt gatcgctaag aaggcataga gcatatactt gagcacatac ttgcgccagt
     1981 atttgcgcag aaattcggcg ttgaactgct cgtcaaactc gtcgatgcga tgctcaatac
     2041 gccactgctc cacaaagtgg ggtacggtgc ggcgcaggat cttgctgacg atatccttga
     2101 cgctgttggc gatgagatcg tggattttat ccttccattt gcggggcagg aacatccatt
     2161 catccacgaa gttcgtcttg tcatagactg tatcccgcac cagtctttcc cacttggcca
     2221 gataaccgtt cttgcggagt ttgtcttcag cctgttccaa atagtcatgc agaatatcgc
     2281 gggctttgtt gaaaacccat tcccgtttgc tgactacgaa tccaggcgtg aagggtatgt
     2341 ggatgcccaa aaactttctg cgcttgagat tgaagagcag ccaatcgacc ttgaggacga
     2401 taatcgtgcc gatcgccata ttccatagga taaacgtaag tgatttgaga aatatcattt
     2461 aaaaacctcc tcccgcaccc ccgccaccag aacgtcctcc tccgaaacca ccgaagccac
     2521 cgaagccgcc aaagcctccg cctccaccac ttgatcctcc accccaggaa ccgccacctc
     2581 cgcgaccgcc cagaatccac atccacaaaa gcacttccag aatgcgaccc cgggtgacga
     2641 tgatcaaaaa gatgaagatc aggacgccaa acagccccac gacgcctttg gccttgtcgg
     2701 ccctgatatt gtttgtgccc ttgctgaatt ccgacacgcc gctgatggtg acaccttttt
     2761 ctctggcaat ccgttgcagc aggagcagca tcccttgagt gaaagctccg tcccagtctt
     2821 cacggccctt gggaagcata tcacgcatgg cgcgatagac gtctccggaa tatgaatcgg
     2881 tgacataggg ctccgaaccg tagcccacct cgatcttgag gcgccgctct ttgagggcca
     2941 gcatgatcag cacgccctca tcccgttggt tgcctatctt ccaggtttca aagactttga
     3001 cggcatggct gttctcatcc atagtaccaa tactttcaaa gatgccaatg gccagctcca
     3061 cgtccgtctt ttccttgagt tcgatagccc agtcgtttag acgcacgcgc gtttcttccg
     3121 agagcagcct ggcatagtcg ttcacaaagc cttggggcgc ggggatttcg ctggcgccca
     3181 ggccgagtgc cagcaaggct aacagcaaca ggggccacaa tttggctcta cacatcgctg
     3241 aggatgtgcc ccatcttgcg tttcttggtt ttgagataat ccaggttttc agcgccggct
     3301 tcgatctcga tcggcacccg ctccactatc tccaggccgt aaccttgcag gcccacaatc
     3361 tttttgggat tgttggtgag caagcgcagc tttttgatgc caagttgttt gaggatttgc
     3421 gctccgatgc cgtaatctcg cagatcggca gggaagccca ggtccagatt ggcctcaacg
     3481 gtatcctttc ctccgtcctg aagatggtag gctttgattt tattcaaaag accgatgccg
     3541 cgtccttcct gcctcatgta gagaatcacg cccctgccct cttccgaaat catttcaaag
     3601 gcccggcgca actggtctcc acaatcgcag cgcagtgagt gcaaagcgtc tccggtcagg
     3661 cattcagagt ggacgcgggt cagtaccgct tgagtgggat cgatcttacc catcacgagg
     3721 gccagatgat attcgtcgct gatagtggag atataggtgt agatgtcaaa gaggccgtat
     3781 ctggtgggga gttttgccac cgagacgcgc tccaccaggc attcctgatg acggcgatat
     3841 cggatcaact ccgcgatcgt gaaaatcttg agggcgtgtt tgcgcgcgaa gatcaccagg
     3901 tcgtccaggc gggccatttc tccatcctcg cggatgatct cacagatagc tcccaccgga
     3961 gctctgcctg ccataactgc aaggtccaca gccgcttcgg tgtggccggc tcgtttcagt
     4021 acccctccct tgtccgccag gaggggaaat atgtggcccg gtctcataaa atccctggct
     4081 tgggagtcgg gatcagctaa acccttgatc gtgagggcac gctcggaagc ggaaatccct
     4141 gtggtggtgc cttccatcgc gtccaccgag acggtaaact tggtgccgtg gcgatcgctg
     4201 ctggaggtag tcatcatggg aatatccaga cgctggagcg cttcttccct catgggaacg
     4261 caaagcaggc ctttggcatg cgagatcata aagtttaccg cttcggtatt gacaaggtct
     4321 gccgccatta caagatcacc ctcattttca cggttttcgt cgtcaaccac aatgagcata
     4381 ccgcccgcgg cgatgatttt caccgcctcg tcaacgctcg cgaagatatt ctctgtatgt
     4441 aagttagtca tctgtatcac tcttattagt catttaaaat agcttccaaa tcaaggcccg
     4501 gtttgtcaat agcaaagttt gagtccagcc agtcgagcgc gtaaagcagg tcttccggca
     4561 ggggagcttt gacgtcgatg ggcttgcggc tgatggggtg cacaaactgc aaacgccagg
     4621 cgtgcagggc ttgacgacgc agatgggtgg tgagtaactc tgtgacccgg cgcttcatgt
     4681 tttgaggcac taacgcgtgg acctgcgccc tggtgttgta aagcaaatct cccaggaggg
     4741 ggtatcccat atgggcaaaa tgaactctga tctggtgcat tctgccagtc tcgagctgaa
     4801 tcttgtgaag ggcaaaatag tgaaaccaat gcacgatttt gtagtgagtc acggcatgtc
     4861 tgccctcggc agcgacacac atcttgcggg gattggtcaa cgagcgggcg atgttggtct
     4921 cgatgctgcc cgcggcctct tcaggaattc cgcaactaac agccagatat gtttttacta
     4981 catcacgata ggcaaacatg tcgttcagtt tgctctgcgc catatcgttt tttgcgatga
     5041 tcatcagtcc gctggtacca ctgtccaggc ggtgcacaat cccaggacga tctggagctc
     5101 ttccgctgga aagctctgag ccccaacgaa ataaaatggc gttcacgaga gtgcgatcgg
     5161 gatttccaaa gccgggatgg acgatcaggc caggctcttt gttgatgatt gccagatgct
     5221 cgtcttcata taccacctcc agagggatat cttgaggcac gatctcctga ggctcgggtt
     5281 ccggcaggct gatgtccact tcgtcgcccg cttggagcag atagcttttt ttaactggaa
     5341 agagattcac caggacgcgg tcttcttcaa tgagcttttc gatgaaggtg cggctgtaga
     5401 gctcttgtat cctcagatcc gagagatact tatccaatct ctgagcttga gtatcctcaa
     5461 acctgatctt aatccgctga cgcattgtcc atgaagtgaa ttacaaagac agcacccgtg
     5521 gctacgcctt tgcggttgcg gatgatagac ccgtcgacgg cagcctggcg gtcttctttg
     5581 tcgtcggcaa cgatgtcctg atagaggttt ttgccatatc gcatggattc tccaatcaca
     5641 tggaaaagcc ggctgtcaaa cgggttggtg aagatcagtt cgttgaggtt gactgtttcc
     5701 gtgatgttgg gataagcttc tcgcagttta tcgttgcaat acactacgtc accgttcacg
     5761 ctgccgatca ggatgccgaa gggcagaatg ttgatcattt gagtgatgcg ctgatggtgt
     5821 tcatagatct tgtccgcctt caccatatca aaacgcatca ggatgttgag cattttttgc
     5881 agcgccatga tgagttcctg gatgtcggca tcctgatcat aggtgctgct gtcgatatcg
     5941 atctgataat taccgtgcga gatctcctct atcagattgc cgatatctct gaaagccctc
     6001 ctgagcctga agggcacata gttgtatatc actatcatat agacaaatac agcaaagaaa
     6061 aagatgatca ggatggattg tacgtcgctg accaaatccg tgaggtcggt ggcctgactg
     6121 agtgtaaacc agatgagacc agactgcagg aggatgatgc ccaaaaggat catgagcaac
     6181 acgtgatagc gtcttctgaa agagcgtttc atggcttaat ccctcctggc ggaagtatcg
     6241 tagatcagct tgtccacatt gcccttggga cccagcagga tgaggacgtc gccctcatta
     6301 accacatcat tggcgccggg catatcgttg agccgttttt cgatcacgtt tctgccgtct
     6361 tcggtcacgg agagatggct gtatttgatg gcgatcacgt tgattccccg tgtgaggggt
     6421 aaagacagct cctgcagagt cttgccaatg aagtctttgg gcgcgatcag ctcgatcatg
     6481 gtatgtccgc tggagaggtt ggtgtattcc aggatgttgg tggagatcag tttcttggcc
     6541 agctgattgc ccacaatctc ttccggcagc aggatgttct ggactccgat gagttccata
     6601 atccggccat gcagccggtt ttccaccttg gcgtaaatca cgctcacgcc aatctttttg
     6661 aggatagcgg cggtgaggat gcactcctga atgttgctgc caatgccaat tattacggca
     6721 tcgacctcgt tgagattgag cgtgcgaagc gtgttttcat cggtggagtc catacagact
     6781 gccgatgcca ccttgccact cacctcatcg atgagttcct ggctcttatc tatggccaat
     6841 acatccattc catattccgc caggatagtg gccaccgtca ttccaaatct gcccagtcct
     6901 attacagcgt aacgggccat gttaatctcc tcattatcct atcgcgatct tttcttcggc
     6961 gtagtccaga ttgatcacat gccgtttgat cgcgagggcg taaatcattg tgagcgggcc
     7021 gattcggccc acatacatca ataaggttat caagacttta ccggtttgac tcagcagcgg
     7081 tgtgatgcca gtggagaggc ccaccgtgcc aaatgccgag acggcttcga aaaagagctt
     7141 atcaaaagga aagggctcca ccagcagcaa gagaaacagg atcacaaaga tgatcgccgc
     7201 ggaaagcgtg atcagggatg tggcctcccg gaagtttgtg gacgggatct tgcggttgaa
     7261 gatgctcaat tcccgcttgc ccttcaggag ggacgtgatc gaaagtgaaa ggaccgcgaa
     7321 ggtggtggtc ttgatccctc cacctgtgga tccgggagac gctcctataa acatcagcgc
     7381 cagcgtgatc aggatactgg ccttgcttag gacactggta tcgattgtgt tgaatccagc
     7441 cgtgcgggta gtgacactct gaaaccagga tgccagcagc cgcttggcta tggtgaaacc
     7501 ttccatcgtg ccatagtatt ccgcccaata gaaggtcacg actcccagca acaacagact
     7561 cccggtagta atcagcacga tcttggagtg cagcgatagc ttcttaaccc tctcgcgata
     7621 aaacaaatac cggttcaaat ccacgatcac ggtgaagccc agcccgccca gaatgatcag
     7681 aaaagtgatg cccaggttca tgacaatatc gctcacatag cccatcatat tttcactcat
     7741 cagggagaaa cccgcgttgc aaaaagccga gacggagtga aacacactca tatagaatgc
     7801 ttccagaggc agataatgag ccgaaaaacg ggtgtagagt aacactgctc ccaggctttc
     7861 aatgagaagg gtcgccagca caatgctttt caagagctgc aggacgttta gagactggct
     7921 gccgcccacc acgtcataca tcacgctttc cagcttgaga gttatcctct ggcccagcat
     7981 gatggcgaag gcggtggaaa tcgtcattat gcccaaaccg ccgatctgga tcagcaccag
     8041 gatgaccagc tgcccaaaga agctgaaatg gctgccggta tcaaccacgg tcaaaccggt
     8101 tacacaggtg gcagatgtgg cagtaaacaa agcgtcaacg atattggtca cttgattgct
     8161 ggatgaggcg gctggcatca tcagcagtat ggtcccgata ctgatcacca gcgcaaaact
     8221 gagcatcaaa gtggcgggag gattggctgt cagctgtttg aagaaacgtc cttcaaacgc
     8281 tttgaagaga aagaactcga ggatatacca ggtctggcgg atcagcaaga agaagatcac
     8341 aaacttggcg tcgaagaaga acagcaaggt gcccagagcc agcatggaac cgtcgaagat
     8401 gagcagattg ttgcctttgc ccggttcgcg ggtgatcaga cggttgatga tcgtgaggaa
     8461 gagaagcagg atgttggcca gagcggtaag gtgttctaca atactgaaat cggcgtagaa
     8521 cgatatcacc gactccataa agagcaccac aaagctccag atcgccagta gataggcgac
     8581 cagctcgatt ctggccagca ggccccgggt gatcgatttc agcatctcag ctccggttta
     8641 gctgacccct tccatcagaa tcctgaatac tgcttccacg ctctttcccg catcgatcct
     8701 ggccagtaat ccatgcgcct caaaccagct ggtgagcccc tgggtttgag cataaaactc
     8761 gatcatgcgt ttacggatgg cttcgggacg gtcgtcctgc cggatgccaa gtttgcctcc
     8821 gcagcggtcg caaacaccct ccttggcagg ctttttgcta ttgaggttaa agttctcgcc
     8881 acaagactga cacactcttc tggctccaat gcgggatatt gcctcttcct cagacagatc
     8941 gaggtagaag accttgcgca cggtgaaatg cttgccgaga tgttcagcct gggcttctgt
     9001 gcgaggaaaa ccgtcgaaca cgatgccttt ggcatttgcc tggatggcgt tggctatgag
     9061 gcggaagatc agctcgtctt ccacaagctc gcctctggcg atcacagctt tgacctcgtt
     9121 gccaagctca gttccacggg acacctggtc tctcaacaga tcgccaatat tgacgtgctg
     9181 gtagcccagt gactcgctca gcagcttggc ctgggtgcct ttaccgctgc cctggatacc
     9241 gaaaatgacg atgcattgca taaaaaccac cttttcggag cagtttccca agccgcggtc
     9301 ctgttgtcaa gaaatctatt tttccaggac cgcaaaagcc gtggcgtatt ccttctcgtg
     9361 agcaatgctg agatggatgg cctgaatgcc aaggctcgcg gcgaaagcat ttgtttcatt
     9421 gtgcaacagc accttaggcg cgccgtcacc ctctctgacg atctcaatat tgagccagtg
     9481 gaccagttcg ttccagccgg tccccaaagc cttcatcaca gcttcctttg ccgcgaaacg
     9541 agccgcgtag gattggtatt tactggccct tgattcacaa taggctgtct ctgccggggt
     9601 gaagagcttt tctccaaagc ggggattggc ttcgatgctc ttttggatgc gctccacttt
     9661 gataatgtca gttccgattc caacgatcat gatcttttct ccagctcctg cagtctaagc
     9721 ttaagctcaa gtccacccgc gaagcctgtc agtttgccac tggctcccac cactctgtgg
     9781 cagggaacga tcaacggcag gggattggcc ctgagggctg ctcccactgc tctggcagcc
     9841 tggggagcac cgatgcgggt ggcgatggca ccataagtgg aggtttcgcc tggaggtata
     9901 tcactggtag cggtatagac cttgcgcgcg aagtcagtca ccctgagggc gtgaggtagc
     9961 tcgagtcgct ggttaatacc gcgtaaatag caatccagca tatctatata aggatgagcg
    10021 gaattgggct cagcggcttc aggaccatcg ggcttgagga agccgacgcg gaccaccaga
    10081 tctccctcga gctcgagctt gaaatggaaa cagccctgat tgtaatagag acttatccgc
    10141 tccgcggggc ttggtgtttg gtttatgtaa ctgtgtttct ttgctttcat ttttgagtcc
    10201 agattgaata tatgcctatc aaggcctgat tgggcctgat ccttgtttcc agcgtccctg
    10261 ccaaattcac ccagcaccgg ccgaatgaag cacgggatgt ttgaggtgaa tcaaccccag
    10321 gcgcaagctc cagggagaag gcggctttca tcagcaatag aggaaggtgt tactgaccat
    10381 aaataaatat ggagcccgct gcttaataag tacacaatcg ggctccataa tgagggtgtt
    10441 atcagcaact aagcagtgaa tctccggcgt tctttgatcc ttccggcttt acctttggcg
    10501 ctgcgcaggt aaaagagttt ggccctgcgg acctggccaa agcgaacgat ctcgagcttg
    10561 tcgatattgg gagagtgttg tgggaaaatc ctctccacgc cgataccgtt cgacacttta
    10621 cgcactgtga aggttttgga gacgccagcc ccgcgtttct ggatcacaat gccctgaaac
    10681 acctggacgc gttctttact gccttcttta attttataat ggaccttcac ggtatcgccg
    10741 acgcggtact cgggaaagtc ggttctgatt tggtctctgc caactgtgtg cagaatatcc
    10801 atggttcctc ccatatatgt tctcggaaac caggcggtcc aaaatgacgg caacggcgct
    10861 gcgcacggat aagtggttat agtttcccgc tccggttatg ggagccagga cgtggtcagc
    10921 ggcagcgtga atctcttcgc atagtccata gccagtgcca aagataagca atatgggcct
    10981 ggccgggagg cgaagagccc gcagatcttc aaatccaatc tgcggctgcc tggtgcgcgc
    11041 cgtcgttgta ataataatgg gacgcgtagc ttcctgcgtt gtaatcattt gtatcagttc
    11101 atcaaagtca tggcatgggc ggataaggtt cagggcctct gagcgctcag ggttgtaggg
    11161 caaatccgga gcttgtttcc agaagtttct gatttcttcc agcagatcga tctggacctt
    11221 atcaggatgc acgatccaat atcccgcgat accataagtc tttgccgctc ttgcgatatc
    11281 gtggatatcc agattggtga gcgaggatgt gatccgggcg ccgttcttat tcagcaccgg
    11341 ataatgcaaa agggcaatat ggatggggct catcgcttag tgagcagttc cgggcgccgc
    11401 gcgtgagtca tagcctggga gcgttcttca gcccaagcct caatcttggc gtgatcgcca
    11461 ttctttaaca cggccgggac ctcgatcccc atccacgtat cgggacgggt ataacaggga
    11521 aatcccagct tggtcttgcc gtctgaaaag gaatcgctcc tggccgaagc ggcatccgtg
    11581 agcactccat cctgaagtct ggctacgcca tcgatgaagg ccattgccgc gatctctcct
    11641 cccgagagca caaaatctcc cagggaaagc tcgtccgaaa tggccagatc tctcacccgc
    11701 tgatcgagtt ccttgtaatg accgcagagt aaaatcaccc gcggcagttg agcatagaac
    11761 tccaggattg gctgagtgag tggacgtccc tgaggactga agtagatcac cggcgcgaag
    11821 ccatcttcaa gcagatgtac gagggcgtcc cagatcggct gcggctgcag cacgagcccg
    11881 ggaaaaccac cataggcgta gtcgtccacc ttgcggtgct tgtccgcgct gaactcccga
    11941 aagtcagtaa agttcagctt cactgcccct ttctggatgg cacgggccac gatgctgacg
    12001 tccttaaagc cgtcaaaacc gcgcggaaag aggctgagca catcgatggt catgggggtt
    12061 cagctatcgc tgtccagcgc gtcaatcaga ggctgtgcgc tctggagcag caacactccg
    12121 ggatcgggaa tctctgcctg gacgtagtgg tccacatagg ggaccagtag ttccttaccg
    12181 gtgggagtgc cgatcaccag cacatattgg gcatggttgt gaaagtaatc cttcacggtg
    12241 cccaaagaac ggtcttcaaa cagcacttca taacccagca ggtgatccag accttcgtcc
    12301 tcgtcgagga attcaggctc aagggcgatc aggacttcgc ggtgcagacg acgctcgata
    12361 tcgatgccgt cctcaataaa gcggaccatg atcttcttgt ctgatacctt tctctcactg
    12421 atagttacgt aaaacactct atcgctttta aagatcaaga agacacactt ggcgtcttcg
    12481 aagacaggcc gataacgagg cttgatcagt acctgaaaaa agccctgggc gtccatgccg
    12541 cccaacctgc cgattccgat cagatcggaa acttccattt attcgataat ttccagctcc
    12601 gcccgctttc cttgtttggt gcttacggcg ttgattatgg tgcggatggc ccccgcggtg
    12661 cgaccgcgct tgccgatcac ttttccgata tcgctctttg agactctcag ttcgtaaagg
    12721 gtgatcctgt ctccggctat ctcggtgatg ttcacttcgc tgggatcgtc aaccaaggct
    12781 ttaaccatga attcgatcaa gtctttcatc tttcacctct tttattgagt atcaatttct
    12841 gtagcggctt gcttggctat cttacgttcg tgccagatct gcagcacacc cgctttacgc
    12901 agcagggagc gtactgtatc gctcggctgg gcaccaacgc tcagccaata gatggcacga
    12961 tcggtctcga tattgatctt ggaagggttg ggcttgggat cataccagcc tacgcattca
    13021 atgtatttgc cgtcgcgttt gacgcgggca tcaactgcca caatgcggta gaagggctgg
    13081 tcgttggcgc ccatccgtct cagtctcagc ttgaccatta tgtctcctta gttattctct
    13141 ataagataat tctgataata tgtccaagaa ttccaagtgg ctattttcgt caagctctat
    13201 tttgggggga gcctacggct ggttagagcc tttggctggt tagagccttt ggctggttag
    13261 agcctttggc tggttagagc ctgcggctgg ttagagcctt tggctggtta gagcactgcg
    13321 tgctggttag aggcagtt
//
LOCUS       DAOSFN010000063        13250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3917_length_13250_cov_8.923986, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000063 DAOSFN010000000
VERSION     DAOSFN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3917_length_13250_cov_8.923986"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            440..721
                     /locus_tag="PLO35_05430"
     CDS             440..721
                     /locus_tag="PLO35_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOH79143.1"
                     /translation="MLKVNSYFEDKVRSIGLDNAEGRSTVGVMEPGEYEFGTSTVEYM
                     TVISGLLTVKLPGETVWKDFGKGQTFVVAANAKFQLKVPEQTAYHCRYE"
     gene            complement(804..1598)
                     /locus_tag="PLO35_05435"
     CDS             complement(804..1598)
                     /locus_tag="PLO35_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH79144.1"
                     /translation="MKTFKVFAGDYWTDGGAMMGVLPWAIWGTRVPTDERRRKKLSLN
                     LLLIVNYDRVILVDSGLGNRLSERQMDIFKPSEFLLPASLAELGYRDVDVTDVIMTHL
                     HFDHAGGLITAFSDGDRLTFPNATVWVQHSEWETAKHPDGLNRAAYDFDHQLALVESS
                     GLLRLIDGSVEIAPGVKVDKVGGHSVGSQIVEIDAGDKFWIYAGDIIPTMFHTSLAIT
                     SAYDICRKDTFRAKQRVYQVLKDRGGYLLLDHDNSQWEIPISELKV"
     gene            complement(1600..2520)
                     /locus_tag="PLO35_05440"
     CDS             complement(1600..2520)
                     /locus_tag="PLO35_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOH79145.1"
                     /translation="MTKIEKTGSSIESVIAAFRREHKIKDWELKYDVVKKPSSGIFGL
                     FANKTAVLRFDLPELQDRVAMFLDRLLTKMGVAFDQIKTKKEGKSVYLEVCGCKDPGF
                     FIGKNGSMLETIQFFLNRIFEADRNLDSIYLDTEGYREKRDSQFLRPFLPMIAKVRGS
                     GKPLTLDPMSASDRRVIHRHVEGQNGLRTLTIGEGDKKRIVIFSSKQSEKEVLSQTGG
                     KAAKKESEHGPDSSPEPRSPHPQRKPQPAQERKFPPRQPRRPRPSTNPEKPDQPSQPE
                     DPAPVETPAPRAPRRRPPRRPSEAKKTQGE"
     gene            complement(2517..4166)
                     /gene="yidC"
                     /locus_tag="PLO35_05445"
     CDS             complement(2517..4166)
                     /gene="yidC"
                     /locus_tag="PLO35_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOH79146.1"
                     /translation="MDKRTLTALLLMFILFLVFQQYVWKPKPVAQTQPTSPEQPAQAA
                     QPAADSLPKPGLALPDSLIDPNLEAARIILSNQVMKVSFSSRGAVIQQVEMKDHKVDK
                     ATPVRLIPPDSGIAGLKVFYPATQSDLSGAVFNYRVSADSSKIDFWLGDEANPKVKRS
                     YSLDSGYGIGMKIRIDNLGTIHGLGLDFGAGIADSETYTKYKAQDYKFVYYANNEIKK
                     TTLASLKKKPAGGKVNTFRWAALRSKYFSIAVKENEPYLTSEFQATVNPKTGNPSMEL
                     ASKSSSGKLIWEQDYLVYAGPADYDLLSGYNSQMQNVAERGPGWLRWLANLIAGFLKF
                     LHGFIPNYGVVIIVFSMILSVILTSIQHPLTRKGMEANLKMQAIQPHMDAIRKRYPND
                     MNAQRVEMAKLQKEHGVNMFGGCIMWVPLLFNMPILISLYSVLRYTLDMRNASFVLWW
                     QDLSLPDKYYVLPILMGATMVIQSRFMKPPSPPEDQMTEQQKQAQQMGKTMNLIMPVM
                     MFFIFRGLPAGLVLYYTVYSIFAAIQQYHLQKKFRNKDTVK"
     gene            complement(4179..4409)
                     /gene="yidD"
                     /locus_tag="PLO35_05450"
     CDS             complement(4179..4409)
                     /gene="yidD"
                     /locus_tag="PLO35_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015961252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOH79147.1"
                     /translation="MTRVTRLPNLLFIALIRFYRMALSPLMVPSCRFYPSCSQYGLEA
                     FRKYNIFKAIGLTIWRVLRCNPFNRGGYDPLP"
     gene            complement(4406..4729)
                     /gene="rnpA"
                     /locus_tag="PLO35_05455"
     CDS             complement(4406..4729)
                     /gene="rnpA"
                     /locus_tag="PLO35_05455"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOH79148.1"
                     /translation="MARLITRHSEYGSLFPPLFYVRSEHFSAAVQVADQEFALGITIG
                     KKIGKAVRRNQLRRRIKAWVRLADSTLPKRFKLNLIARPGAGELSWPQLCDQLQALVQ
                     KLGNQ"
     gene            complement(4733..4867)
                     /gene="rpmH"
                     /locus_tag="PLO35_05460"
     CDS             complement(4733..4867)
                     /gene="rpmH"
                     /locus_tag="PLO35_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011708966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOH79149.1"
                     /translation="MKRTYQPSNRSRKNTHGFRSRMATHNGRKVLARRRAKGRKTLSV
                     "
     gene            complement(4988..6205)
                     /locus_tag="PLO35_05465"
     CDS             complement(4988..6205)
                     /locus_tag="PLO35_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH79150.1"
                     /translation="MELAELKYWERIWNDYEKDPATAVALLGIFRLLSNPDRKLTEYG
                     QSPSAIGSSPFNHLFDEALSLREVANRVLKLVKLKPETGKTIKTVIKGVLEPSSKSGS
                     SFEVAIKDSQIRQIGLKRKKVNNNAHQATPPVSGAPVVSWTSRPVNGMMPNLYQTVKA
                     AVLDGAFSTPPFQIRIEGRHWRYPVYQRKQSFNIMLVLDVSNSVRWVLKFMEQIIGML
                     TAQANACKDKLGLIIFNDDRAHVMHYPTSNTRHVIGTINTLSPKGKTPLADGMRLAFQ
                     TLEQSRFRVTGMSNAIVLLSDCFPEPIVGGYEDQLDEPVCQQILALCDKISEAAIKLL
                     VISPAIKGLPDYEKHIGYRLGKLAAERAKGNFLSLMAEPTGSVFSDQESYRIPEASVK
                     ELMREIGGFRMDQ"
     gene            complement(6304..7431)
                     /locus_tag="PLO35_05470"
     CDS             complement(6304..7431)
                     /locus_tag="PLO35_05470"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79151.1"
                     /translation="MTKLRFPFMAIIGQEELSLALILNLVNPRIGGVLIRGTKGTGKS
                     TAVYGLPQIAPQIQVHQDCAYNCSPDEPEKWCATCKGRQENPIIQKEIDVINLPMNIS
                     EENLSGRIDVEQLLKRGEKVFVPGLIAKAHRQILYIDEVNLIPDHVTDSLLDVVASGV
                     NIVEREGFSVEHPARFLLIGTMNSEEGELRPQILDRFPLSVTIQTVSNLEDREEIIRR
                     NFAYEEQPETFLASFRDVSELMKTSILEARKLLPETTISQNLTRKVLDFCVHHNIDGH
                     RSEIVIVKAARAYAALELKRQVEQKHVMLAMKITLLHRTREGGLKKPVTYSDIEAWWQ
                     DSKDTSGVDQDFSLQYDPENLSLLKGGYVNPSKKSGTVSRD"
     gene            complement(7460..8173)
                     /locus_tag="PLO35_05475"
     CDS             complement(7460..8173)
                     /locus_tag="PLO35_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008198401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOH79152.1"
                     /translation="MNLSDEMVQILQAEKDPLRAVGTAKFFQAQPGGYGEGDQFWGIK
                     VPIQRKISRIWCAQLSADELASLLSHPVHEVRLTALMALVLLFQRAGAKDQKALIVEL
                     YLANSAGVNNWDLVDSSAHQILGEWLCDKDWSLLTELAVSPELWKQRIAMIASYAFIK
                     KGAFGPTLALARLLIDHRHDLIHKAVGWMLREVGNRDQQVELSFLKDHYHFMPRTMLR
                     YAIEKFPEALRQDFLTGRA"
     gene            8355..8744
                     /locus_tag="PLO35_05480"
     CDS             8355..8744
                     /locus_tag="PLO35_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79153.1"
                     /translation="MKQIYKTMLWVGGSTLVAYQGWRAAKAIMGVRKLDKALPEFLEN
                     IYGERPKVDINLMMNVSTVMVIKLSFSADLLTKSPDIEDEVRDYIRDFYPGLAKYKTR
                     IKLMPTEEPAPDHETAVASEPLVEPVD"
     gene            8809..9582
                     /locus_tag="PLO35_05485"
     CDS             8809..9582
                     /locus_tag="PLO35_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOH79154.1"
                     /translation="MDITLWSWNVNGLRAVLSKDFISVINTEKPDILGLQETKLQEHQ
                     IPEELTNLSGYRDFWSHAHRKGYSGTAVFSAFDPLKAEYGIGKEEFDTEGRVVILEYE
                     KFIFFNVYFPNGQKDEERLDYKLRFYDRMLEVMQAKRATGKMVIVTGDYNTAHHPIDL
                     ANPKENEKTSGFLPIERAWLDKISSLGWIDTFRLFHPEPQRYSWWSYRAGARPRNVGW
                     RIDYFWVDAEHQAWVKDADIRDDIMGSDHCPVTLKITLP"
     gene            complement(9569..10351)
                     /locus_tag="PLO35_05490"
     CDS             complement(9569..10351)
                     /locus_tag="PLO35_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOH79155.1"
                     /translation="MRSIMIWLLLVCVTGLSAATKMTVAEFEQEIFRLTNEQRAKYGL
                     AALQPEAGLAELARRHGRNMAANDFFEHKDPEGLEVGGRKKKYYSQLMTHSIGENLAF
                     FEHSLKRFSPSKVVNGWMNSPGHRANILEPGYTHLGVAVIVTGNKLYAVQNFARPIVR
                     LLSSLPKSYSQNKVYRLEFLYMDSQDARNLEVLLNLPDATAEVPIGGNMYVVGVQPLN
                     ITWGEGKRFSVDVPFQYGKGIYSLAFGWGGGHYNSEFIFKVK"
     gene            complement(10362..10820)
                     /locus_tag="PLO35_05495"
     CDS             complement(10362..10820)
                     /locus_tag="PLO35_05495"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOH79156.1"
                     /translation="MRDTYKLLAMVLLLGFITPCLGQSKPQPTRAMLYSALLPGGGQV
                     YNRAWVKAGLVVGVQGWLIGSAIYNDNKKDEFHNLASSAAQSNDQLYYKAMETQYRNR
                     FNNDIWWIGITAALSMVDAYVDAHLSDFEEQEKKLRLRFSETGLSLQYRF"
     gene            10996..13107
                     /gene="fusA"
                     /locus_tag="PLO35_05500"
     CDS             10996..13107
                     /gene="fusA"
                     /locus_tag="PLO35_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH79157.1"
                     /translation="MSSDADSPLHKIRNIGIMAHIDAGKTTTTERILFYSGFLHKMGE
                     VHDGNTFTDWMEQERERGITITSATVTCLWKDKRINIIDTPGHVDFTAEVERSLRILD
                     GAVGVFCAVGGVEPQSETVWHQADRYHIPRLAYVNKIDRSGADYERAIQMIHERLTPK
                     AIPINIPMGMEDKNEGIVDLITMKLWTFDPLTQGGITQLREIPADFLEEAERQREALL
                     EAVAEYDDELMELFLEARTIPEEIIRRAIRNGTIHHGLIPVLCGSSLKNKGVQLLLDA
                     ICDFLPSPLEVEECTGFHPATNDALCVVPDPASPFVALAFKVQIDKYVGKLVYIRVYS
                     GTLKKGNTIVNQTNGKRERVARILQMMSNRKTDLDELHAGDIAALVGTRFLITGDTIT
                     DGTVDIHLSKMHFPDAVISIAIEPKTKADQENLGNALARLEEEDPTFKVHIDKDSGQT
                     LISGMGELHLDIIVDRLRREFNVSANVGNPQVSYKETISRTVTGEEIFQREMNGKGNY
                     AHVVFRLSSLALSDLDGEEKNRFRIAVPAEKIPSEYWKAIEEAALNALNDGPLISGNV
                     ERVLVELIDGSFNPVDSNELAFRIATSLAMGKALRASEPGIMEPVMLLTVLSPEDFVG
                     DIIADINAKRGRIGILRHHNEFQQEIVAEVPLSELFGYATRVRSISQGRAVYTLEFKK
                     YEITPSSIQSSILKRIRGYAE"
BASE COUNT         2981 a         3444 c         3648 g         3177 t
ORIGIN      
        1 gagctgcgca tgtgagatac ctgcggtagg tgagatacct gcggtaggtg agatgagccc
       61 cgttggagtt caagcggcca cgataaagct atgcgtgaca gcgagtttac gggccgcttg
      121 gacttcaacc agcacatcgc aggaatgaca caaccatctc aaccacctca agtacctcaa
      181 ccctcccgac atgataaccc gctacttttc cccagccgga tatcccgcag gcatctcagc
      241 cctatcccat gcctaagcct gggataggcg tgcgataagc gtgcgataag cacgggatag
      301 tgaagaggaa gacggagcaa tcccgccaac gttgatctat tagcggcagt cctgggaaag
      361 agggtctgat tattgtgctt tacaaatctc tcggggtggc aaaaatggca attatcaaaa
      421 acatcccaag gagttcatca tgctgaaagt aaacagctat tttgaggaca aagtgcgttc
      481 cataggcctg gacaacgctg aagggcgttc caccgtggga gttatggagc cgggagaata
      541 tgagttcggt accagcacag tggagtatat gaccgtgatt tcaggtctgc tcaccgtgaa
      601 gctgcccggt gaaaccgtct ggaaggattt tggcaaaggt cagaccttcg tggtggccgc
      661 gaatgccaaa ttccagctca aagtgccgga acagacggcc taccattgtc gttatgagtg
      721 atagatcaac ggggagcttc aggctccccg ttctttttca aatggtgatt agttgtttac
      781 aagggtctgg aggggcgtcc cgcttagact tttaactccg atatggggat ttcccattgg
      841 ctgttgtcat ggtccagcag cagatagcca ccgcgatctt tgaggacctg atagacgcgc
      901 tgtttggccc ggaaagtatc cttgcggcag atatcgtagg ctgaagtgat ggccaaagaa
      961 gtgtgaaaca tggtggggat gatatctcca gcataaatcc agaatttatc acccgcgtcg
     1021 atctccacga tctggcttcc gacgctgtgt ccgcctacct tatcgacctt gacgccggga
     1081 gcgatttcca cgctgccgtc gatcaacctc agcaggcctg agctttccac cagggccagc
     1141 tgatggtcaa aatcataagc ggcgcggttg agtccatccg gatgtttggc agtttcccat
     1201 tcggagtgct ggacccagac agtggcattg ggaaaagtga ggcggtcgcc atcactgaag
     1261 gctgtgatca gccctccggc atggtcgaag tggagatgag tcataatgac atcggtgacg
     1321 tccacatccc ggtaacccag ctccgccaat gaagctggca ggagaaactc cgagggctta
     1381 aagatatcca tttggcgttc gctgagcctg ttgcccaaac cgctgtccac caggattacc
     1441 cggtcgtagt tgacgatcag cagcagattg agggacagtt ttttgcggcg tctttcatcg
     1501 gtgggcaccc tggtgcccca aatggcccat ggcaacaccc ccatcatggc gccaccgtct
     1561 gtccaatagt cgcccgcaaa cactttgaag gttttcactt tactctccct gggtcttttt
     1621 agcctcactg gggcgtctgg gaggacggcg tctgggagct ctgggagcgg gggtttccac
     1681 tggggccgga tcttcaggct gggatggctg atcaggtttc tcaggattgg tcgagggcct
     1741 ggggcgacgc ggctgtctgg gtgggaactt gcgttcctga gccggctgag gctttctctg
     1801 aggatggggg cttctgggtt ccggactgct atcgggaccg tgttcagatt cctttttcgc
     1861 ggctttgcct cccgtctggg aaagtacttc tttctctgat tgtttggagg aaaagatcac
     1921 gatgcgcttt ttatctccct ctccgatcgt gagggtgcgc agtccgtttt ggccttccac
     1981 gtggcgatgg atcactctgc ggtcggaagc gctcatgggg tccagggtga ggggtttgcc
     2041 actgccgcgg accttggcga tcataggcag gaagggacgc aggaactggg aatccctctt
     2101 ctcgcgatag ccctcggtgt ccagatagat gctgtccaga ttgcggtcag cttcaaaaat
     2161 gcggttgaga aagaattgga tggtttccag catagagccg tttttcccga tgaaaaagcc
     2221 gggatctttg cagccgcaaa cttccaggta aacagacttg ccttcttttt tggttttgat
     2281 ttggtcaaac gccaccccca tcttggtgag cagcctgtcc agaaacatcg ccacccggtc
     2341 ctgaagctcg ggcagatcaa atcttaaaac ggcggtttta ttggcgaaga ggccaaaaat
     2401 cccggaactg ggttttttga cgacgtcgta tttcagttcc cagtctttaa tcttgtgttc
     2461 tctgcggaag gcggcgatca ctgattcgat gctgcttccg gtcttttcaa ttttggtcat
     2521 ttaacagtgt ccttgttacg aaactttttt tgcagatggt attgctggat ggccgcgaaa
     2581 atgctgtaaa cggtgtagta taacaccagt ccggccggca atcccctgaa gatgaagaac
     2641 atcatcaccg gcatgatcag gttcatggtc tttcccatct gctgggcttg tttttgctgc
     2701 tcggtcatct ggtcttccgg tggagagggc ggcttcataa agcggctctg aatcaccatg
     2761 gtggcaccca tcaggattgg caggacgtag tatttatccg gcagcgagag gtcctgccac
     2821 cacaacacga agctggcgtt gcgcatgtcc agagtgtagc gcagcacgct gtagagggag
     2881 atcaggatcg gcatgttgaa gagcagcggc acccacatga tgcagccgcc aaacatattg
     2941 accccgtgtt ccttctggag tttggccatc tcgacccgct gggcgttcat atcgttggga
     3001 taacgtttgc ggatggcgtc catgtggggt tggatggcct gcattttgag gttggcctcc
     3061 atgcctttgc gggtgagggg atgctggatg gaggtgagga tcacggaaag gatcatcgag
     3121 aagacgatga tgaccacccc gtaattggga atgaagccat gcaggaattt gaggaagccg
     3181 gcgatcaggt tggccagcca tctcagccat cccggtccgc gttccgccac gttttgcatc
     3241 tggctgttgt aaccagacaa gaggtcgtaa tctgcgggac ccgcgtagac cagataatcc
     3301 tgctcccaga tcagcttgcc gctggagctc ttggaggcaa gttccatgga gggatttccg
     3361 gttttggggt ttaccgtggc ctggaactcg ctggtgaggt agggctcgtt ttccttgacg
     3421 gcgatgctga aatacttgct gcgcaaggcg gcccaacgga aggtgtttac tttgccgccg
     3481 gcgggtttct ttttcaggct ggccagggtg gtttttttga tctcgttgtt ggcataatag
     3541 acgaacttat agtcctgagc tttatacttg gtataggttt cgctgtccgc gatccctgcg
     3601 ccaaaatcca gaccgaggcc atgaatggtg cccaggttgt cgatcctgat tttcatgccg
     3661 atcccgtagc cggaatcgag gctgtagctg cgcttcactt tgggattggc ttcgtctccc
     3721 agccagaaat cgatcttgct ggaatcggca gatacccgat agttgaacac ggccccgctc
     3781 agatcgctct gggtggcggg ataaaacact ttgagccccg cgatcccgga atctggaggg
     3841 atgaggcgaa cgggtgtggc cttgtccacc ttgtgatctt tcatctccac ctgttggatc
     3901 acggctccac ggctggaaaa gcttactttc atgacctggt tgctcaggat gatccgggcc
     3961 gcttccagat tggggtctat caggctgtcc ggcaaagcca atccaggttt gggaaggctg
     4021 tccgcggcag gctgagcggc ctgagcaggc tgctccggtg aagtgggctg ggtttgagcg
     4081 acgggtttgg gtttccagac atactgctgg aagaccagaa acaggataaa catcagcagg
     4141 agggcagtta aagttctctt atccaattga ttgcctcatt atggaagggg gtcgtatcca
     4201 ccccggttaa aaggattgca gcgcagcacc cgccagatcg tgagccctat tgctttgaaa
     4261 atgttgtatt tacggaaggc ttccagacca tattgactgc agctgggata gaagcggcag
     4321 ctgggtacca tgagcggaga gagggccatc cgatagaatc tgatcaaagc gatgaacagc
     4381 aggttgggca gacgggtgac gcgcgtcatt gattgccaag cttttgcacg agggcctgca
     4441 gttgatcgca gagttgtggc catgataatt ctccggctcc cggacgcgct ataagattga
     4501 gcttaaagcg cttgggcaag gtggaatccg ccaggcggac ccaagctttg atccggcgcc
     4561 tgagctgatt gcgccgcact gctttcccga tctttttgcc gatcgtgatc cccaacgcga
     4621 attcctgatc agcgacctgg acggccgccg agaagtgttc agaccggaca tagaacagcg
     4681 gagggaacag gctcccatat tcgctgtgtc gcgttattaa acgcgccatg gctcatacgc
     4741 tgagagtctt tcttcctttg gcgcggcgac gggcaaggac cttgcgaccg ttgtgagtag
     4801 ccatgcggct gcggaacccg tgggtgtttt tgcgggaccg gttggaaggc tgatatgtgc
     4861 gtttcattta taaagctcct tgattgatat aggtatttgt gcttgaaaag ccacgatttt
     4921 cacgggggct ctggtgtcaa gccaaatctt ggtacttgga cagagggcct gccagtgaaa
     4981 ggccgtgcta ttggtccatc ctgaatcctc caatctctct catcagttct ttgacgctgg
     5041 cttcggggat gcggtagctt tcctgatcag agaacacaga acccgtgggc tctgccatca
     5101 ggctcaggaa attgcctttt gccctttctg cagccagctt tccaagcctg tagccgatgt
     5161 gtttttcata gtccggcaga cctttgatgg cgggactgat caccaggagt ttgatggcgg
     5221 cttccgagat tttatcgcat agagccagaa tttgttgaca gacaggctca tcgagctgat
     5281 cttcatatcc gcccacaatc ggttcgggaa aacaatctga gagcaacaca atggcgttgg
     5341 acattccggt gactctaaac ctgctctgct ccagagtctg aaacgccagt ctcatcccgt
     5401 ccgcgagggg agttttgccc ttgggagaga gagtgttgat cgtcccgatc acgtgtctgg
     5461 tgttgctggt gggataatgc atgacgtggg cgcgatcatc gttgaaaatg atcagtccca
     5521 gcttgtcttt gcaggcgttg gcctgagcag tgagcatccc gatgatctgc tccataaact
     5581 tcagtaccca tctcacggaa ttgctgacat ccagcaccag catgatgttg aagctctgtt
     5641 tacgctgata gactggatag cgccaatggc gtccctcaat tctgatctgg aagggaggag
     5701 tggaaaatgc tccatccaga acggccgctt tgacggtctg atagaggttt ggcatcatgc
     5761 cattgaccgg gcgggatgtc cacgagacca ctggcgcacc tgagacagga ggggtggcct
     5821 gatgcgcgtt gttattcact ttcttgcgtt tcaaccctat ttgtctgatc tggctatcct
     5881 tgatcgccac ctcgaagctg gaaccagact ttgaactggg ctccagcaca cctttgatca
     5941 cggtcttgat ggtcttgcct gtttccggtt tcagcttcac cagctttaga accctgttgg
     6001 ctacctctct caggctcaag gcttcatcaa agaggtggtt gaagggagaa ctgcctatgg
     6061 cagaggggct ttgaccatac tcagtgagtt tccggtccgg atttgacagc agtctgaaga
     6121 tccccagcag ggccacggcg gtcgcggggt ccttttcata atcgttccag atcctttccc
     6181 agtatttcag ctctgccagt tccacctcag tcaggggttg attctggact ttcttttcta
     6241 tcagccttac cagattacgc cttacggcat aagcatcgtc agcctcgcgg gccagaatca
     6301 gatttaatcc cgcgagactg tcccggattt ttttgatggg tttacatagc cccctttcaa
     6361 caaactcaga ttttccggat cgtattgcag agaaaagtcc tgatcaaccc cggaagtatc
     6421 tttggaatcc tgccaccagg cttcgatatc ggagtaggtc acaggttttt tgagtccgcc
     6481 ttcccgggtg cggtgcagga gcgtgatctt catggccagc atcacgtgtt tttgttctac
     6541 ctgccgctta agttccaaag ccgcgtaagc cctggcggct ttgacgatta cgatctcgga
     6601 gcgatgaccg tcgatgttgt gatgcacaca gaaatccagg accttgcgtg tcagattttg
     6661 ggatatcgtg gtctcaggca gcagtttacg cgcctccagg atggaagttt tcatcagttc
     6721 actcacatcc ctgaaactgg cgagaaaggt ctccggctgc tcctcgtagg caaaattgcg
     6781 tctgatgatc tcttccctgt cctcaagatt gctgaccgtc tggatggtga cggaaagggg
     6841 aaagcggtcc aggatctggg ggcggagctc accctcttcg ctgttcatgg tcccgatgag
     6901 caaaaaccgg gcaggatgct ccaccgaaaa gccttcccgt tccacgatgt taactcccga
     6961 ggccaccacg tccagcagac tgtccgtgac gtggtcgggg atcaggttga cctcgtcgat
     7021 atagagtatc tgtctgtgcg ccttggcgat aagacccgga acaaagacct tttcccctct
     7081 tttgagcagt tgttccacat cgatgcggcc cgagaggttt tcctctgaga tgttcatggg
     7141 cagattgatc acgtctatct ctttctggat aatggggttt tcctgtctgc ctttgcaggt
     7201 ggcgcaccat ttttccggct cgtcgggtga gcagttgtaa gcgcaatcct gatgaacctg
     7261 gatctgcggg gcgatttgag gcaggccata aaccgcggtg cttttgccgg tacccttggt
     7321 gccacgaatg agcacccctc cgatgcgggg attgaccaga ttcaagatca aggccaggct
     7381 gagctcctcc tgtccgatga tagccataaa cggaaacctg agttttgtca tcctttctcc
     7441 tttgtttagt gattgattcc taagcccttc cggtgaggaa gtcctgtctg agcgcttcgg
     7501 gaaacttttc tatcgcatag cgcaacatgg tgcgcggcat gaagtggtaa tggtccttca
     7561 gaaagctgag ctcaacctgt tggtcccggt ttcccacctc tctcagcatc cagccaacgg
     7621 ctttgtggat gagatcgtgc cggtgatcga tcagcagcct ggccagcgcc agagtgggtc
     7681 caaaggcacc tttcttgatg aaagcgtagc tagcgatcat ggcgatcctc tgtttccaca
     7741 gctctgggga aacagccagc tccgtcagca agctccaatc cttgtcacac agccactctc
     7801 ccaggatttg atgcgcgctg gaatccacca ggtcccagtt gtttacgccg gcagagttgg
     7861 ccagatagag ctccacaatc agggctttct ggtcttttgc tccggcccgc tgaaacaaga
     7921 ggaccagagc catcagagcg gtgagacgga cttcatggac gggatgggac agcaagctgg
     7981 caagctcatc cgcggaaagc tgcgcgcacc aaattcggct gatctttctt tggatgggca
     8041 ccttgattcc ccaaaactgg tcgccttctc catagccgcc tggttgagct tgaaaaaact
     8101 tcgcggtgcc cacagccctg aggggatctt tctccgcctg gaggatttgg accatctcgt
     8161 ctgacaagtt catcgtaact cctctggttc tggatatttg ggcaggctgt caagctatgc
     8221 gggatttgtc aatgcttttg tttgctcctt tccaggtaag tgatggaaaa acttgctcta
     8281 cttccggaga gacttataat gtccctatac tagaataatg atcgacgggg ctagttcccg
     8341 tgtggaggta tatcatgaag cagatttaca agaccatgct ctgggtggga ggatcgaccc
     8401 tggtggccta tcagggctgg cgcgccgcca aagctattat gggagttcgc aagctggaca
     8461 aagccctgcc ggagtttctg gaaaatatct atggcgagcg tcccaaggtg gatatcaatc
     8521 tgatgatgaa cgtctcaacc gtcatggtga tcaagctgag cttcagtgcc gatctgctta
     8581 ccaagagccc agacattgag gatgaagtaa gagactatat ccgggatttc tatccaggtc
     8641 tcgccaagta taagacccgc atcaagctca tgcccacgga ggagccagcc ccggatcacg
     8701 agaccgcggt ggcctccgaa ccgctggtgg agccagtgga ctgatccgca agacaattaa
     8761 gctgaattgc ggtctgaata ctaacacgcc ataggagcac aaccattgat ggacatcact
     8821 ctctggagct ggaacgtgaa cggcctgagg gctgtattga gcaaagactt catctctgtg
     8881 atcaacactg agaaaccaga catcctgggc ttgcaggaaa ccaagctgca ggaacatcaa
     8941 atccctgagg aacttacgaa tctgagcggc taccgggact tttggtctca cgcccaccgc
     9001 aaaggttatt caggaacggc cgtcttctct gcctttgacc ctctcaaggc agaatatggc
     9061 atcggcaaag aggagtttga caccgaaggc cgcgtcgtga tcctggagta tgagaagttt
     9121 attttcttca acgtttattt ccccaacgga cagaaagacg aggagcggct ggattataag
     9181 ctgcgcttct atgaccggat gctggaagtg atgcaggcca aacgggctac cggaaagatg
     9241 gtgatcgtga cgggagacta caacaccgcc caccatccca ttgacctggc caatcccaaa
     9301 gaaaacgaaa agacctcagg atttttgccg atcgaacggg cctggctgga taaaatttcc
     9361 tcccttggct ggatagacac tttccggctt ttccatccag agccccagcg ctattcgtgg
     9421 tggagctaca gagccggcgc caggcccaga aacgtgggct ggagaatcga ttatttctgg
     9481 gtggatgcag agcaccaggc ttgggtaaaa gacgccgata tcagagacga cataatgggc
     9541 tctgatcact gtccggtgac cctgaagatc actttacctt aaagataaac tccgagttgt
     9601 agtgtccccc gccccagccg aaagccaggc tgtagatacc tttgccatac tggaagggca
     9661 catcgacgct aaagcgcttc ccctcccccc aggtgatgtt gagcggctga actcctacca
     9721 catacatatt tcccccaatg ggcacctcgg cagttgcatc cggcaggttg agcaacactt
     9781 ccagattgcg ggcgtcctgg ctatccatgt ataagaattc cagccggtaa accttgtttt
     9841 gagaatatga tttgggcagc gaggacagga gcctgacaat cggacgcgca aagttttgca
     9901 cggcgtagag cttgttgccg gtcactataa ccgccacccc caaatgagtg taaccaggct
     9961 ccagaatatt ggctcggtgg ccgggtgaat tcatccaacc gttcactacc ttggaggggc
    10021 tgaaacgttt gaggctgtgc tcaaagaagg ccaggttttc tccgatgctg tgggtcatca
    10081 attggctgta atacttcttt ttgcgccctc ccacttccaa accttcgggg tccttgtgct
    10141 caaagaaatc gttggcggcc atgttgcggc cgtggcgtct ggccagttct gccagaccgg
    10201 cttccggctg caaggctgcc agaccgtatt tggccctctg ttcgttggtg agcctgaata
    10261 tttcctgttc aaactcagcg acggtcattt tggtcgcagc gctcaggccg gtcacacaca
    10321 ccaacagcag ccagatcatg attgatctca tcatatctcc ttcagaacct gtattggagg
    10381 ctcaggccgg tttccgaaaa ccttagccgg agcttttttt cctgctcttc aaagtcactt
    10441 agatgcgcgt ccacataggc atccaccata gaaagcgctg ccgtgatccc gatccaccag
    10501 atgtcgttgt tgaaacggtt gcggtactgg gtctccatag ctttataata tagctggtca
    10561 ttggactggg ccgcgctgga cgcgagattg tgaaactcgt cttttttatt atcgttgtaa
    10621 atggcactgc caatcagcca tccctgcacg ccgaccacca gaccggcctt gacccaggca
    10681 cgattgtaga cctgtccccc gcccgggagc agggcagaat agagcatagc tctggtgggc
    10741 tggggtttag actgccccag gcagggcgtg ataaaaccaa gcagcagaac catggccagc
    10801 agcttgtatg tgtctctcat tctcatgttt ggcattttac acaaggcccc gctttgtcaa
    10861 ctaaattctg ccccgcctct cccagaacga tctttctggg gctcagaggc atttttttat
    10921 ggacaaagag cggcgattgt tatttctggc tttgatcgtt tacaaacggt aaatactaat
    10981 agcatggaaa ggattatgag ctccgacgcg gacagtccgc tgcacaagat cagaaacata
    11041 ggtatcatgg cgcatatcga cgccgggaaa accaccacca ccgaacggat cctcttctat
    11101 tcaggattcc tgcacaagat gggtgaagtc catgacggca acacctttac ggactggatg
    11161 gaacaggagc gggaacgcgg gatcacgatc acctcagcca ccgtcacatg cctctggaaa
    11221 gacaagcgga tcaacatcat agacactccc gggcatgtgg atttcaccgc tgaggtggaa
    11281 cgcagcctga ggattttgga cggcgcggtg ggagtttttt gcgcggtggg aggagtggag
    11341 cctcaatcag agacggtctg gcatcaggcg gaccgctatc acatcccgcg gctggcctat
    11401 gtaaacaaga tcgatcgcag cggggccgat tatgaacgcg cgatccagat gatccatgag
    11461 cgcctgactc ccaaagccat tcccatcaac atccccatgg gcatggaaga caagaacgaa
    11521 ggcatcgtcg atctcatcac gatgaagctc tggacctttg atcctctcac ccagggtggg
    11581 atcacccagc tcagagaaat cccggctgat tttcttgagg aagccgaacg gcaacgggaa
    11641 gctttgctgg aagcagttgc ggagtatgac gacgaactga tggagctatt tctggaagcg
    11701 cggaccatcc ctgaggagat catccgccgg gcgatcagaa acggcactat ccatcatggc
    11761 ctgatcccgg tgctctgtgg atcttcgctt aaaaacaaag gtgtccagct gcttttagac
    11821 gcgatctgcg acttcttgcc ttcccccctc gaggtggaag aatgcaccgg ttttcatccc
    11881 gctaccaacg acgccctctg tgtggtccct gatcccgctt cgccctttgt ggcactggct
    11941 ttcaaggtgc agatcgacaa gtatgtgggc aagctggtct atatcagagt atattccggc
    12001 acccttaaaa aaggcaatac gatcgtcaat cagaccaatg gcaagcgtga acgcgtggcc
    12061 agaatcctgc agatgatgag caaccgcaag accgatctgg atgaactgca cgctggagac
    12121 atcgcggctt tggtaggaac ccgctttttg attacgggag atacaattac ggatggcacg
    12181 gtagacatcc acctctccaa aatgcacttc ccggatgccg tcatttccat cgccatcgag
    12241 cccaagacca aagcagatca ggaaaatctg ggcaacgcgc tggcccgtct ggaagaagaa
    12301 gatcccactt tcaaagtgca catcgataaa gacagcggtc agaccctgat ctcaggtatg
    12361 ggagagttgc atctggacat catcgttgac cgtctgagaa gagaattcaa cgtgagcgcc
    12421 aacgtcggca accctcaggt ttcatataaa gagacgatct ccaggaccgt gaccggtgaa
    12481 gagattttcc agcgggagat gaacggcaaa ggcaactacg cgcatgtggt gttcaggctt
    12541 agttcattgg ccctcagcga cctcgacgga gaagaaaaga atcgcttccg gatcgcggtc
    12601 ccagccgaga aaatcccctc tgaatactgg aaggcaatcg aagaggctgc cctcaatgcc
    12661 ctcaatgacg ggcccctgat cagcggtaat gtggaacggg tgttggtaga gctgatagac
    12721 ggctccttca accccgtgga ttccaatgag ctggccttcc ggattgccac ctcactggcc
    12781 atgggcaagg cgctcagagc ctctgaaccg gggatcatgg aaccggtgat gctgctcacc
    12841 gtgctgagcc ccgaagactt tgtgggagat atcattgccg atatcaatgc caaacgtgga
    12901 cgcatcggca tcctgcgcca tcataacgaa ttccagcagg aaatcgtggc agaagtgccg
    12961 ctcagcgagc tctttggtta tgccacccga gtccgttcca tcagccaggg aagagccgtt
    13021 tacacactgg aattcaagaa atacgagatc actccttcca gcatccaaag cagcatcctg
    13081 aaacgcattc gcggttacgc ggaataaact cttgacactg aaatggcttg ggatagaata
    13141 ggtttttttg agaattgaga attgagaatt gagaattgag aattgccgcg taccatgttt
    13201 ggcgtaacgc ttagccaata acaaacagac caaatattaa aggagtaagc
//
LOCUS       DAOSFN010000064        13241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3921_length_13241_cov_8.979145, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000064 DAOSFN010000000
VERSION     DAOSFN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13241
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3921_length_13241_cov_8.979145"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..2270
                     /locus_tag="PLO35_05505"
     CDS             183..2270
                     /locus_tag="PLO35_05505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOH79158.1"
                     /translation="MSSPSNPLLWDKNDHRLKAIPFPEFKTEHFLPAIEAALEEAKAE
                     IEAIKRDPAPPTFENSILALELAGERLNHVAGIYFNLHSAHSDADFKALAQQISPLLA
                     QYSVSISTDPDIFARVKAVYEAEVAGQPKPSLPKDFKDKDALKKAERYRLIDRSYTGF
                     IRGGALLSDEQKKRFTEISMELSKLSPQFSDNVLAAMNAFELHITDPADVEGMPEGVL
                     ASAAHTAKQKGKSEGWVFTLQPSSMVPLVTYCRNRSLREKINRAYGSRAFRDQFDNQE
                     IILKTLRLKKERAALLGYETHADYVISDRMAASHQNVTGFLDKIYKVAYPAAQQEVAE
                     VAALAKELDGLDELMSWDFGYYSNKLKEKLFAYDPEELRPWFKVENVLEGLFVVAERI
                     YGIKVKQVTDVPVYHPDVTTWEVHDADGNYLGLMYMDLFPRDTKRGGAWMTSFQSQGL
                     HKDGVRRPMVSIVASLTPSTPERPSLLRMDEVRTIFHEFGHGLHGLLADGYYHSLAGT
                     SVLWDFVELPSQIMENWLLEEEALGLFARHYESGEPLPKELLDKVIAARNFQAGVANV
                     TQLRYASMDLKWHSSDPHSIQDVDAFEKEATAAYQLMPAVEGSNTSCAFSHIFAGGYS
                     AGYYSYKWAEALEADAWSLFQEKGIFDQETAAAFRKYILARGNAFHPMDLFEAFRGRK
                     PDPDALLKRDGLL"
     gene            2527..2907
                     /gene="gcvH"
                     /locus_tag="PLO35_05510"
     CDS             2527..2907
                     /gene="gcvH"
                     /locus_tag="PLO35_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOH79159.1"
                     /translation="MLVLDDLYYTESHEWIRVQDGLATIGITDFAQHELGDIVFVELP
                     DMGAKVSADEPCGSIEAVKAVEDIICPVSGKVEERNGDLEDSPDMINKSPYEDGWLIK
                     VRMSSPEELEKLLSALDYRKIVEA"
     gene            2935..3549
                     /gene="gmk"
                     /locus_tag="PLO35_05515"
     CDS             2935..3549
                     /gene="gmk"
                     /locus_tag="PLO35_05515"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOH79160.1"
                     /translation="MIAKSKNFLIILSAPSGGGKSTILSEVRKLNSEVDYSVSYTTRA
                     PRGTEQNGIHYHFVDEAEFCRRKDEGDFLEHANVFGKWYGTSISYIQSRLDARKHVIM
                     DIDVQGAALIGETSIPYVKIFIIPPSMDVLRERLINRGTDSRDEINKRLNIARNELKY
                     IPSYQYLVINDDLAQAVQEVLAIIKAEENRVNRYHNPVSEFLGE"
     gene            3554..3967
                     /locus_tag="PLO35_05520"
     CDS             3554..3967
                     /locus_tag="PLO35_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79161.1"
                     /translation="MIDKKIENFLEKTDMNYLFCLLSKLEAGRLSQLPVYVRNKLGEK
                     LSVLSMQHVSDNEIPDYISEIAEAELAMAQAQSPFDDDDGSDSDLGEETYDDSLRLTD
                     EPEPEPVNPFDVVDDDDDEDIFGDQDDLDEADDDD"
     gene            3967..4620
                     /locus_tag="PLO35_05525"
     CDS             3967..4620
                     /locus_tag="PLO35_05525"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOH79162.1"
                     /translation="MSNRAIRQHLELLLNSGIRELYEPSSQARTSLEQLANQYANCQK
                     CALHEGRVKFVYGEGDPHAGIMVIGEGPGEQENLSGRPFVGRAGDLLNKMLAAINLRR
                     EQVYIANIVKCRPPGNRNPLPEERIACLPYLIEQIQIIQPQIFLMMGLVAAQTLLSTN
                     EAMGVLREREHVFMDRPAFVTYHPAALLRNEHWKRPAWEDLQRFQKAWLEMASGQRP"
     gene            4703..5470
                     /locus_tag="PLO35_05530"
     CDS             4703..5470
                     /locus_tag="PLO35_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH79163.1"
                     /translation="MCLAVQGLAGTNLEARIAIYGDTRGDNATHARVVKAISTFSPDL
                     VFHTGDLSTQGKSADEFDAFKSVIKALGNAEFYPVRGNHERDEQLFLSYFTQLCGSSR
                     YVLERDSLVFIALDSSIGIKPGSEQLVWLQQQLFRHQDQPVILLVHHPVFSSGAHGDE
                     LGLGVWLPALLENSSVKAVISGHEHSYERSEYQGIPYLVSGGGGSPFRETRNPNPYSR
                     LFIISHHFIIMDREGPVLKCRVFDPDLKLLDSFDIDL"
     assembly_gap    5495..5594
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5919..8705)
                     /locus_tag="PLO35_05535"
     CDS             complement(5919..8705)
                     /locus_tag="PLO35_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79164.1"
                     /translation="MKKSWLIIIALMIASAMFAYEVVAWQEDFETGATGWTHYDGAQS
                     PNNWHVYDNGGTQGNCWWMGDPALASGTNIGGYYDHQYLVLDTPARTLTAGNATLTFK
                     LRHNLEDPAGATAPYTGWDSANVRMSVDNGVTWTPISGTPAYNITSSYAFGFEHGEGP
                     NIPAWGGVQANWTNATFDLSAYVGQSVKIRFAFASDPAYSTGDAPAMYGVMVDDIAFG
                     GYSNNGVDDGQMTWSSLVPLGGDLWAIATDPAAPSPTHIMKCQNASGSYNVNMLNYLV
                     TPPITLPSSGDIRADFMIMGGFTDPNTFPEVDYFGWEISNNNGQTWYAMSNPYGNPAG
                     TNYVYSDAPDVWSSMTASYSVDGYISDYAGQTIQLRWYFKSDADTPSGIGIMIDDVKI
                     FNDVFIAAPENLTATVAGSNVTLNWTTPGGGGGGEPGWLSYDGENAGNSIGTNAAADF
                     DVAAKWDPVGEHGISPWVGMNVTKIKFFPAEPTTLCAYTVRLWTGATGNLAYEQAVAN
                     PTIDAWNEIVLTTPWTIPSGTIVMAGYRCNATGGYPAGCDEGPQVEGYGNVIRFNNAW
                     TTLTALGATLTYNWNIRIYVEDANGREYEITQLPTNEQHNSGTLAVSTSERSNRDVTA
                     YKIYRDQVQIDQVAGTVLTYTDMNVEGGMHTYHVTAMYGANESLPSNIVTTFVMPAMH
                     TELSHDDGTAEQGYTVGSTRQMAVKHNFNQSVTVKYAKIFVHTPGSAGIIVRVFDNDG
                     TGGMPGTQMAQYQYPAASITPGWNYVTLPAGIIAADGQFYIAILETTNASQIGLDTAS
                     NGFSYTRTGTTWDPLTTGELMIRAIVEYTTDNDDNVAPVFTLDARNFPNPFNPETTIS
                     FSVPVSGPTTLKVYNLKGQVIRTLVNDAREAGRHSVVWNGKDDNGNSVSSGMYFFRVH
                     NDGKHVTRKMLLSK"
     gene            complement(8843..11005)
                     /locus_tag="PLO35_05540"
     CDS             complement(8843..11005)
                     /locus_tag="PLO35_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79165.1"
                     /translation="MHFGGGGGRGGFQSDDIKGKIYDRRLYGKLLAYLKPYLKWVILS
                     FAILMLVAGAEVVLPLIQRTAIDDHIVSDRSIAVFENGSVLEDFYTKYKSLKLQRFES
                     GNRGFLILRAKDRNRIDKLDLDRLREQGYLSAKTVYILEDKPQHIEVLNRYMSEVSAP
                     HGRKGGLNGWFRIGNAKLAVPRDLLNKMPGKDRLAVRAESAHQLVWLALIFFGIILLR
                     LISGYSQSVLTAYFSQMAMNDLRHDVFAHLQKMPVKYFDQNPVGRLVTRVTNDIRAID
                     EMLASGVITIVQDAILIVAIVALMLVLNWKLALVSFIILPFVIFMISQFRRRTRVIYR
                     EVRKHLAALNATLAEHISGQKIIQLFNQYSNKRNEFADINQQYFATSFKQLRLFAFFR
                     PIIHVSSQIAVALIIWYGGGQILRNVITIGLLMAFTQYIHKLFEPINDFSEKFNVLQG
                     ALAGAERIFDLMEEEPEDYRSDKLRNLKLEGGIEFDHVWLAYNPGEWVLRDVSFKIKP
                     GEKIALVGHTGSGKTSIVNLMLGMYPYQRGEIRIDDRPLSSYAIKDVRSNIGIVQQDV
                     FIFSGNVHDNIALNNDKLSSEDIIKVAKYVNADRFISQLPAKYEEPVMERGATLSTGQ
                     RQLIAFARVLAYDPSIFILDEATSNIDTETEILIQDALKKIIEHRTSIMIAHRLSTIQ
                     HVDRILVLHKGELVEEGSHFELLDKKGLYYDLYRLQYT"
     gene            complement(11005..12756)
                     /locus_tag="PLO35_05545"
     CDS             complement(11005..12756)
                     /locus_tag="PLO35_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79166.1"
                     /translation="MRTFDAILPYLKKSYGKIAWGIVMLILVDVVQLAMPKVMQYAID
                     GIQERRVDQNGLIWVGLIIFGLAIAVMVLRYFWRTLIIGNSFVIERHLRQDFYDHLLR
                     MSQNFFNRSKIGDLMAHATNDLTAVRMLFGMGLIAAMDIILMTIASFSFMGAINWRLT
                     ALAVIPLPFLSITIGYFGKRMHKTFGRVQASFSELSGRIQESISGIRVVKAFVQEESE
                     LKKVDAVSLDFVKQNIKLARISGIFHPFMSFVISLSVIITLLFGGRAAIRGEISIGEF
                     IAFFQYLSMLVWPMIAIGWIVDMYQRGTASLKRLNEIFTVNPEITDAEADHSITRLEG
                     GIEIGDLNFRYGEELPLVLKHVTTSIANGKTLAIVGPTGCGKTTLIELLARVYNPPAG
                     SIYIDGHPLHSIPLKVLRRDMVLVPQDIFLFSETIADNIRLGNPDSSLESVYEAARVA
                     QVYDEIMEFEHKFETIVGERGLTLSGGQKQRVAIARALLTDPQVLILDDALSAVDTKT
                     ERYILEKLIEVRRGKTTIIIAHRISSIQHADHIIVLGAGDVRESGDHNSLLKLNGLYR
                     DLFEKQRIRAKLEGEEL"
BASE COUNT         3078 a         3495 c         3614 g         2954 t
ORIGIN      
        1 ccttcggcct tcgggttatg agcccgacga gctaccagct gctccacctc gcgtcaatgt
       61 tctgccacaa aatccggggc ggggtttttg tcaagaactt tctgcgccag cctttttctt
      121 gcgcgctaaa gccgcccgga ttatcttgtc tcaaaaagta tcattgcaaa aaaggaattc
      181 atatgagctc accctccaat cccctccttt gggacaaaaa cgaccaccgt ctcaaagcta
      241 ttccctttcc cgagtttaag accgagcact tccttccggc catcgaagcc gctctggaag
      301 aggccaaagc cgagatcgag gcgatcaagc gcgatcccgc tcctcccact tttgaaaaca
      361 gcatcctggc cctcgaactg gctggcgaac ggctcaacca cgtggccggc atctatttca
      421 acctccattc cgcccactcc gacgccgatt ttaaagccct cgcccagcag atttctcccc
      481 ttttggccca gtacagcgtg agcatttcca ccgatccgga tatctttgcc agagtgaaag
      541 ccgtctatga agcggaagtc gctggccagc ccaaaccctc cctgccaaag gacttcaagg
      601 ataaagacgc gctaaagaaa gccgaacgat atcgcctcat agaccgcagc tacactggtt
      661 tcatcagggg cggagcgctg ctttctgatg aacaaaaaaa gcgttttact gaaatctcca
      721 tggagctttc caagctaagt cctcaattca gcgataacgt cctggctgcc atgaatgcct
      781 ttgaactgca tatcaccgat cctgccgacg tggaagggat gccggaaggc gttttggcct
      841 ccgcggccca tacagccaaa caaaaaggca aatccgaagg ctgggtcttc acccttcagc
      901 cttccagcat ggtgccgctg gtcacctact gccgcaaccg ttccctgcgc gaaaagatca
      961 accgcgccta tggctccagg gctttcagag atcagtttga caaccaggag atcatcctca
     1021 agaccctcag gctcaagaaa gaaagggccg ctctgctggg ttatgagacc catgccgatt
     1081 acgtgatctc agaccggatg gccgccagcc atcaaaacgt aacaggcttt ctggacaaga
     1141 tttacaaagt ggcctatccc gccgcccaac aagaagtggc ggaagtggcc gccctggcca
     1201 aagaactgga tggcctcgat gaactgatgt cctgggattt tggctattat tccaacaagc
     1261 tcaaggagaa gctctttgcc tacgatccgg aagagctgcg tccctggttc aaagtggaaa
     1321 acgtgctgga aggcctcttt gtggtggcgg agcgcatcta tggcatcaag gtgaaacagg
     1381 tgacggacgt tccggtctac catccggatg tgactacctg ggaagtccac gacgccgatg
     1441 gcaactatct gggcctcatg tatatggacc tcttcccgcg ggataccaaa cgcggagggg
     1501 cctggatgac ctccttccag tctcagggct tgcacaaaga cggggtgcgc cgtcccatgg
     1561 tctcgatcgt agcttcgctc acaccctcca ctccagagcg gccgtcgctg ttgaggatgg
     1621 atgaagtgcg caccatcttc cacgagtttg gccacgggct gcatggtctg ctggcggatg
     1681 gttattatca cagcctcgcg ggcaccagtg tgctgtggga tttcgtggag cttcccagcc
     1741 agatcatgga aaactggctg ctggaagaag aggctctggg gctctttgcc aggcactacg
     1801 aaagcggtga accgctgcct aaggaactgc tggacaaggt gatcgcggcc agaaacttcc
     1861 aggccggcgt ggccaatgtc acccagctcc gctatgccag catggacctc aaatggcaca
     1921 gcagcgaccc gcatagcatc caggacgtcg acgccttcga aaaggaagct acggcagctt
     1981 atcaactgat gcccgcggtg gaaggctcca atacttcctg cgctttcagc cacatcttcg
     2041 ccggtggata ttccgcgggc tactacagct acaaatgggc cgaagccctc gaagctgacg
     2101 cctggagcct ctttcaggaa aaaggcatct ttgaccagga aactgccgcc gccttcagaa
     2161 agtatatcct ggcccgcggt aacgcgtttc atcccatgga cctctttgaa gccttccggg
     2221 gccgcaaacc tgatccggac gccctgctga aacgggacgg actgctgtag tttactgatg
     2281 attttcccgc agataacgca gatttagacg cggattacgc agatagtaat gaagtagcgc
     2341 tgcaatgagc gtcgatactt ctcaaacctc tcaacctctg cgagatcagc gcttaaatct
     2401 gagaaatctg cgggaaagct tgtgaaatct gcggggaacc cgagcaccca agttctgctt
     2461 gacacaaact gccccccggc agacttggct cactatatta tatcacaccc gcgtatggag
     2521 gataaaatgc tggtattaga cgatctttac tacaccgaga gtcatgaatg gataagagtc
     2581 caggacggac tggccaccat cgggatcacc gattttgccc agcacgaact gggagacatc
     2641 gtttttgtgg aattgcccga tatgggtgct aaagtctctg ctgatgagcc ctgcggttcc
     2701 atcgaagccg tcaaggcggt ggaagacatc atttgcccgg tttccggtaa agtggaagag
     2761 cgcaacggcg atctggaaga cagccccgat atgatcaaca aatctcccta tgaggacggc
     2821 tggctgatca aagtgagaat gagctctccg gaagagctgg aaaagctgct gagcgcgctc
     2881 gattatcgca agatagtgga agcctaggcc ttataaactt tcagcccagc gtttgtgata
     2941 gccaaaagca agaatttcct gatcattcta tccgctcctt cgggtggagg caagagcacc
     3001 atcctctctg aggtgcgcaa gctcaactcc gaggtggact actcggtttc ctacaccacc
     3061 cgcgccccca gaggaacgga gcaaaacggt atccattatc actttgtgga cgaggcggag
     3121 ttttgccgcc gcaaggatga aggcgacttt ttggagcacg ccaacgtctt tggcaagtgg
     3181 tatggcacct cgatcagcta tattcaaagc aggctcgatg cccgcaaaca cgtgatcatg
     3241 gatatcgacg tccagggtgc cgctctcatc ggtgaaacca gcatccctta tgtgaagata
     3301 ttcatcatcc cgccctcgat ggatgtcttg cgggagcgtc tgatcaaccg gggcaccgac
     3361 agccgcgatg agatcaacaa acgcctcaat attgcccgca acgagctcaa gtatatccca
     3421 tcatatcaat atcttgtgat caacgacgat ctggcccagg ccgtgcagga agtgctggcc
     3481 attatcaaag ccgaagaaaa ccgcgtgaat cgttatcaca accccgtttc tgaattccta
     3541 ggagaataag cccatgatag ataaaaagat cgaaaacttc ctggaaaaga cggacatgaa
     3601 ctacctgttc tgcctgcttt ccaagcttga ggccggccgc ctctctcaac tccccgttta
     3661 cgtgcgcaac aagctgggag aaaagctttc cgtgctctcc atgcagcacg tttcggacaa
     3721 cgagatcccg gattacatca gtgagatcgc ggaagccgaa ctggccatgg cccaggccca
     3781 atcacccttc gacgatgatg acggctccga cagcgatctg ggagaagaga cctatgacga
     3841 ctccctcagg ctgactgatg aacccgagcc cgagcccgtc aatccctttg acgtcgtcga
     3901 tgatgacgac gatgaggata tctttgggga ccaggacgat ctcgacgagg cggatgacga
     3961 cgactgatgt ccaaccgggc catcagacaa caccttgagc tgctgttaaa cagcggcatt
     4021 cgcgagcttt acgaacccag ctcccaagcc agaacctctc tggaacagct cgcaaaccaa
     4081 tacgccaatt gtcagaaatg cgctctgcat gaaggccgcg tcaagtttgt gtatggagag
     4141 ggcgatcccc atgccgggat catggtgatc ggagaggggc ctggtgaaca ggaaaacctg
     4201 agcggcagac cctttgtggg cagggccggt gaccttctca acaagatgct ggccgccatc
     4261 aatctgcggc gagagcaggt ctatatcgcc aacatcgtca aatgccgtcc tcccggcaac
     4321 cgcaatccct tgccggaaga gcggatagcc tgccttccct acctgataga gcagattcag
     4381 atcatccagc cccaaatctt cctgatgatg ggcctggtgg ccgctcagac cctcctgagc
     4441 accaatgagg caatgggcgt tctgagagag cgggaacacg tcttcatgga ccggcctgct
     4501 tttgtgacct atcatcccgc cgcgctgctc agaaacgagc actggaaacg tcccgcctgg
     4561 gaagacctgc agcgctttca aaaagcctgg ctggaaatgg cttccggaca gcgcccttaa
     4621 ccgttgaact aaagctatgc caagtgtaaa cggagccatc cccaagcaac ttaggtttgg
     4681 gtgggagatg ctgttcctga gcttgtgctt agccgtccag ggcctggcag gcaccaatct
     4741 ggaggccagg atcgcgatct atggagacac ccggggagac aacgcgaccc atgccagagt
     4801 cgtgaaggca atatccacct tttcgccgga cctcgttttc cacaccggag atctcagcac
     4861 tcagggtaaa agcgcggatg agtttgacgc tttcaaatcg gtcatcaaag cgcttggaaa
     4921 tgcggaattc tatcccgtga gaggcaacca cgagcgggac gaacagcttt tcctcagcta
     4981 tttcacgcag ctctgcggca gcagcagata cgtgctggag agggattcgc tggtctttat
     5041 cgctttggac agcagcatcg ggataaaacc cggatcagaa cagcttgtct ggctgcagca
     5101 acagcttttc aggcatcagg accagcctgt gatcctgctt gtgcaccatc ccgtcttcag
     5161 cagtggagcg catggcgacg aactgggact gggcgtctgg ctgccagcgc tgttggaaaa
     5221 cagctctgtg aaagcagtga tcagcggcca cgagcattct tatgagagat ctgagtatca
     5281 gggcatccca tatctcgtct ccggaggggg tggctcacca tttagggaaa cacgtaaccc
     5341 caatccctac agcaggctgt tcatcatcag ccaccatttc atcatcatgg accgggaggg
     5401 ccccgtcctc aaatgcaggg tttttgatcc agatctgaag ctgctggata gctttgatat
     5461 cgatctttaa tttcatctgc taaacttggg gtttnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnaactcc aacggggcag ctcttttcac gcaccgacta aagtcagtgc
     5641 tacggccggc taaagccagc gttacgatct cacggctgat ctttcctggt caaagagctc
     5701 tcaatgctct cgttccgtct gtctttatct cattcttccc aataacttgc cgtgacaata
     5761 ccgtgcgctc tcccgcgatt ttcaccggag gccccacggt atcccgacgg caacaaatgg
     5821 gagagtaaac agcggtaaaa taaaaacccc ggtccgaaga ccggggccga tatgtcagag
     5881 actggcgtga ggccacgtct ccgttttaat gatggggttt atttggacag gagcatcttg
     5941 cgggtgacgt gctttccatc gttgtgaacg cggaagaagt acatgccgct ggagacgctg
     6001 ttgccattgt catctttacc gttccagacc acgctgtgac gaccggcttc gcgggcatcg
     6061 ttgacgaggg tgcggatcac ctggcccttg aggttgtaga ccttgagggt ggtgggacca
     6121 ctgacgggaa cgctgaagga gatggtggtt tcagggttga aggggttggg gaagttgcgg
     6181 gcatccagtg tgaagacggg agccacgttg tcatcgttgt cggtagtgta ttccacgatc
     6241 gcacggatca tcagctcacc agtggtgagg ggatcccagg tggtgccggt gcgggtatag
     6301 ctgaagccgt tgctggcagt atccagaccg atctgggagg cgttggtggt ctccaggata
     6361 gcgatataga actggccatc ggccgctatg attccggcag gcagggtcac atagttccag
     6421 ccgggagtga ttgaagcggc gggatactga tactgagcca tctgggtgcc gggcatgccg
     6481 ccagtgccgt cgttgtcaaa cacgcggacg atgattccag ccgatccggg agtgtggacg
     6541 aagatcttgg catacttgac ggtgacgctt tgattgaagt tgtgtttcac ggccatctga
     6601 cgagtcgagc ccacggtgta gccctgttcg gcagtaccgt catcgtggct gagctcagta
     6661 tgcatggcag gcatcacgaa agtggtcacg atgttggaag gcagcgattc gttagcgcca
     6721 tacatggcag tgacgtgata agtgtgcatt ccaccttcca cattcatgtc ggtgtaggtg
     6781 agcacggtgc cggctacctg gtcgatctgg acctggtcgc gatagatttt gtaggcggtg
     6841 acgtcacggt tggagcgctc ggaagtgctg accgccaggg tgccgctgtt atgctgttcg
     6901 ttggtaggca gctgagtgat ctcatattca cggccgttgg cgtcttccac atagatgcgg
     6961 atattccagt tgtaggtgag ggtggcgcca agagcggtga gggtggtcca ggcattgttg
     7021 aaccggatta cgttgccgta accttccacc tgaggacctt catcgcaacc ggcaggatat
     7081 ccgcctgtag cgttgcagcg gtaaccggcc atcacgatgg tgccggaagg aatggtccag
     7141 ggagtggtga ggacaatttc gttccaggca tcgatggtgg gattggcaac agcctgctcg
     7201 taagcaaggt ttccagtggc accagtccag aggcggacgg tgtaggcgca aagagtggta
     7261 ggctcggctg ggaagaattt gatcttggtg acgttcattc ccacccaggg gctgatacca
     7321 tgctcgccga cgggatccca tttggcagcc acgtcaaaat cggctgcggc attggtgccg
     7381 atggaattac cggcattttc gccatcatag ctcagccagc caggttctcc accaccaccg
     7441 ccaccaggag tggtccagtt gagggtgaca ttggagccgg cgactgtagc cgtaaggttt
     7501 tcgggagcgg cgatgaagac gtcgttgaag atcttgacat cgtcgatcat gattccgata
     7561 ccgctaggag tatcggcatc gctcttgaaa taccagcgca gctgaatggt ctggccagcg
     7621 tagtcgctga tataaccatc cacgctgtag ctggcagtca tggaactcca cacatccgga
     7681 gcatcggaat acacatagtt ggttccggcg ggattgccat aaggattgct catggcatac
     7741 caggtctgtc cgttattgtt tgagatttcc caaccaaaat agtcaacttc ggggaaggtg
     7801 ttgggatcgg tgaaaccgcc catgatcatg aagtccgctc tgatgtctcc actggaggga
     7861 agcgtgatgg gaggagtcac caggtagttg agcatattta cattgtagct gccggaggcg
     7921 ttttggcatt tcatgatatg agtgggtgag ggagcggcgg gatcggtagc gatggcccaa
     7981 aggtcaccac ccagcggaac gaggctggac caggtcatct gaccgtcgtc aacgccattg
     8041 ttgctgtatc cgccaaaggc gatgtcatca accatcacac catacatggc aggagcgtca
     8101 cccgtgctgt aagctggatc ggaggcaaag gcaaagcgaa tcttcacgct ctggcccaca
     8161 taagcgctga ggtcaaaggt agcattggtc cagttggctt gcactccacc ccaggcaggg
     8221 atgttggggc cttcaccatg ctcgaaaccg aaagcgtagg aagatgtgat gttgtaagcg
     8281 ggagtcccgg aaatgggggt ccaggtcaca ccgttatcca cggacatgcg aacgttggca
     8341 gagtcccatc cggtgtaagg agctgtagcg ccggcgggat cttcaaggtt gtgacgcagt
     8401 ttgaaagtga gagtggcatt gccagcggtc aaggtgcggg cgggagtgtc caacaccagg
     8461 tactgatggt cgtaataacc accgatgttg gtgccggaag ccagagcggg gtctcccatc
     8521 caccagcagt taccctgggt gccgccattg tcatagacgt gccagttgtt tggtgattgc
     8581 gcaccgtcgt agtgggtcca tccggtggcg ccggtttcaa aatcttcctg ccatgctacc
     8641 acttcgtagg caaacatggc cgaagcgatc ataagggcta tgatgataag ccagcttttt
     8701 ttcattgatc ctcctatgaa tcaagagata ttttcataaa cagctatata cgcaagccgc
     8761 gtgattttgt caacactttt ttatagtgtt gtagtattat aagcatgatt cgagccatat
     8821 ccactgcctt gatatccagc tcttaggtgt attggagcct gtaaaggtcg taatacaggc
     8881 ccttcttgtc cagcagctca aagtgagagc cttcctccac caattcacct ttgtgcagca
     8941 ccaggatgcg gtccacgtgt tgaatggtgg aaaggcggtg ggcgatcatg atcgaggtgc
     9001 ggtgttcgat aatctttttc agggcgtcct ggatcaggat ctcggtttcg gtatcgatat
     9061 tggaggtggc ctcatccaga atgaagatgg agggatcgta ggccagcact ctggcgaagg
     9121 cgatcaattg acgctgtcca gtggacaggg tggcacccct ttccatcact ggttcttcat
     9181 atttggcggg gagttgcgag ataaaccggt cggcattgac gtatttggcc accttgatga
     9241 tgtcttcgct gctgagcttg tcgttgttga gggcaatgtt gtcgtggacg ttgccgctaa
     9301 agataaacac gtcctgctgc acgatcccga tgttggaacg gacgtctttg atagcatagc
     9361 tgctgagggg acggtcatcg atgcggattt cacctctctg atagggatac atgcccagca
     9421 tcaggttcac gatcgaggtc ttgccgctac cggtgtggcc cacgagggct atcttctctc
     9481 ccggcttgat cttgaagctc acatccctga gcacccactc gcctggattg taggccagcc
     9541 acacgtggtc aaactcgata ccgccttcca gcttgagatt gcggagctta tcagagcggt
     9601 agtcctcagg ctcttcttcc atcaggtcaa agatgcgctc agctcctgcc agggctccct
     9661 gcagcacatt gaacttctca gagaagtcgt tgatgggctc aaagagtttg tggatatatt
     9721 gggtgaaggc catcagcaaa ccgatggtga tcacgttgcg caggatctgc ccgccgccat
     9781 accagatgat aagagcgacg gcaatctgag acgacacgtg gatgatcgga cggaaaaagg
     9841 caaagaggcg caactgcttg aaagatgtgg cgaaatactg ctggttgata tcggcaaatt
     9901 cgttgcgttt gttggagtac tggttaaaaa gctggatgat cttttggccg ctgatgtgtt
     9961 ctgccagagt ggcattcagg gcggcaaggt gctttctgac ttcacgatag atcactcggg
    10021 tgcggcgtct gaactgggag atcataaaga tcacgaaagg caggatgata aagctcacca
    10081 gggccagttt ccaattgagc accagcatca atgccacaat cgccacgatc aggatggcgt
    10141 cctgaacgat ggtgatcaca cccgaagcca gcatctcatc gatggcgcgg atatcgttgg
    10201 tgactctggt gaccagcctg cccaccggat tctggtcaaa gtatttcact ggcatctttt
    10261 gcagatgggc aaagacgtcg tgccggaggt cgttcatagc catctgcgag aaataggctg
    10321 tcagaacgct ctggctataa ccgctgatca atctgaggag gatgatccca aagaagataa
    10381 gcgccagcca gacaagctga tgagcggatt ctgcccgcac tgccagacgg tctttgccgg
    10441 gcatcttgtt cagaagatcg cgcgggacag ccagcttggc gttgccgatc ctgaaccatc
    10501 cgttcaggcc gcctttacgt ccatggggcg cgctgacctc gctcatatag cggttcagca
    10561 cctcgatgtg ctggggttta tcttccagga tgtaaacggt cttggcgctc aggtagccct
    10621 gctcgcgcag ccggtccaga tcgagtttgt cgatgcggtt gcgatctttg gctctcagga
    10681 tcaaaaatcc cctgttgccg ctctcgaaac gctggagctt gagacttttg tacttcgtgt
    10741 agaaatcttc cagaacactt ccgttttcaa aaacggcaat ggagcggtcg gacacgatat
    10801 gatcgtcgat agcggtacgc tggatgaggg gaagcaccac ttccgcgccg gccacgagca
    10861 ttaaaatcgc aaaagagagg atcacccatt tcagataggg tttcaggtag gcaagcagct
    10921 tgccgtaaag acggcggtcg tagatcttgc ctttgatgtc gtcagattgg aagcctccgc
    10981 ggcctcctcc acctccaaag tgcattatag ctcctcccct tccagtttgg cgcggatccg
    11041 ctgtttttca aacaggtcgc gatacagccc gttcagcttg aggaggctgt tatgatcgcc
    11101 gctctcacgc acgtcaccag ctcccagcac gatgatatga tcggcgtgct ggatggaaga
    11161 gatacggtga gcgatgatga tggtggtctt gccgcggcgt acttcgatca gcttttccag
    11221 gatgtaacgc tcggtcttgg tatccacagc tgagagggcg tcgtccagaa tcaggacctg
    11281 aggatcggtc aaaagcgctc tggcgatggc cacgcgctgt ttttgtcccc cggaaagagt
    11341 gagtccccgc tctcccacaa tggtctcaaa cttgtgctca aattccatga tctcgtcata
    11401 gacctgcgcc acccgggccg cctcatagac gctttccaag ctgctgtcgg gattgccgag
    11461 gcggatattg tcggcaattg tttcggaaaa gaggaagata tcctgaggca ccagcaccat
    11521 gtctctgcgc aatactttga gagggatgct atgcagggga tggccgtcga tgtaaatgct
    11581 gcccgcggga ggattataaa ccctggccag cagctcgatc agagtggtct tgccgcatcc
    11641 cgtgggtccc acgatggcca gcgtcttacc gttggctatg ctcgtggtga cgtgtttcaa
    11701 caccagcggg agttcttcgc catagcggaa gttgaggtcg ccgatctcga tgccaccttc
    11761 cagacgggtg atgctgtggt cagcttcggc atcagtgatc tctggattca cggtaaaaat
    11821 ctcgttgagt cgcttcagag atgccgtgcc gcgctgatac atatccacaa tccatccaat
    11881 ggctatcatc ggccacacca gcatactgag atactggaag aaggcgatga attcaccgat
    11941 gctgatctct ccacggatgg cagcccggcc gccaaagagg agggtaatga tcacagaaag
    12001 gctgatcaca aagctcataa agggatggaa gataccggag atcctggcca gcttgatatt
    12061 ctgcttcaca aagtccaaag agacggcatc cacctttttg agctcagact cttcctgcac
    12121 gaaggctttt accactctga tgccggaaat agactcctgg attctaccgc tcagctcaga
    12181 aaaactggcc tgcactctgc caaaggtctt gtgcatgcgc ttgccaaaat agccgatggt
    12241 gatggacagg aagggcagcg ggatcaccgc cagggccgtg agccgccaat tgatggcacc
    12301 catgaaactg aaggaagcga tcgtcatcaa tatgatatcc atcgcggcga tgagtcccat
    12361 cccaaacagc attctcacag cagtcaggtc attggtggcg tgggccatca aatcgccgat
    12421 cttgctgcga ttgaagaaat tctggctcat acgcagaagg tggtcataaa aatcctgccg
    12481 caagtgacgc tcgatcacaa aggaattgcc gatgatgaga gtgcgccaga aatagcgcag
    12541 caccatcacg gcaatggcca gaccaaagat gatcaggccc acccagatga ggccattctg
    12601 atcgacccgc cgctcctgga tgccatcgat ggcatattgc atcaccttgg gcatggccag
    12661 ctgcaccacg tccaccagga tcagcatcac tatgccccag gcgattttgc cataactctt
    12721 cttcagataa ggaagaatgg cgtcgaaagt tcgcattaat gctccgtcgt cgtattttat
    12781 cgactcaagc cgcgctgaag ctctgtgatt gtctgcaatc atcgtggatt gagtggataa
    12841 aaccctattt tgagctgagg gacaggctca gatgcaggcc cattttgtca acaacaatct
    12901 gagccgcaga tatatcagca attcacatga cagcatgaac aaaacttcac tctcagatat
    12961 acatgaacca ggttcttgcc ggatgcgtta atttgatcca ataatatact aatatcattg
    13021 cgcattcaac ttgtaatcat cccatgtatg gaagcaaagc tacagattct ccccaaactg
    13081 ttatggagag ccgaaaaaag cgcttgagca aaagcaataa gtgtcatcag ctttgttcag
    13141 gaaacttgtc aggcgtggac aattctgcgc tattttatga taatcaacta gtgtctcgtc
    13201 aagcatcgat tgtcggatag agacgcttca gttttgttct t
//
LOCUS       DAOSFN010000065        13186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3941_length_13186_cov_9.384129, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000065 DAOSFN010000000
VERSION     DAOSFN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3941_length_13186_cov_9.384129"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLO35_05550"
     CDS             <1..756
                     /locus_tag="PLO35_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79167.1"
                     /translation="PAPQPVQVRKEASGMVYVAGGSFEMGNNTGRQDERPVHTVTLSA
                     FYMAKFPVTQEQYQAVMKLNPSQFKGNKHPVEKISWREAIRYCNMLSKIEGLKPCYLT
                     GSSGMSPDNPECDWLANGYRLPTEAEWEYAARGGVYAQKGGPVATLDLRQEGGKTNDA
                     MHMTQAVGLAPANELGITDMLGNVNEWCWDWYMVEYYAHSIARNPIGPMQGMYKVCRG
                     GSWKNLSMSVTTRFSFELNQFYNNVGFRVVRSG"
     gene            753..1205
                     /locus_tag="PLO35_05555"
     CDS             753..1205
                     /locus_tag="PLO35_05555"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOH79168.1"
                     /translation="MSVSNSVKHQLQDPGRSLIRPVPNFIDSFRFLSLLFLLILTGFL
                     LLFRFTEPGALGPGFRCPVHQYTGLLCPGCGSMRALHSLVHGHFIPALRANFMMVLLL
                     PGLLYSFAYGLLTGREPGSNPRLKARLSNMLIIVLLVFTIGRNLLGGV"
     gene            1205..2353
                     /locus_tag="PLO35_05560"
     CDS             1205..2353
                     /locus_tag="PLO35_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOH79169.1"
                     /translation="MFYPGDSIQGFTIIRQLGKGGMGEVYLGEDAHLARKVAIKELSS
                     ALGSDESFTQRFKHEAQLQARLSHPNIVSLYSFFEEGGRYYMVLEYAEGITLRDLIRQ
                     TGPVPYQRATTIIRQVLEALEYAHRQKIIHRDIKPVNIIVGANDQVKILDFGISKIMG
                     ERGLTGTGQMLGTVNYMSPEQVKALKDIDGRSDLYSLGVTFFEVLSGRLPYDTLTDSD
                     YEIMNQIVQAPMPDPRSYYPGIPEKTVAILFKMLAKDRSQRYSSAWEVMQDFEAPVVS
                     MPVQAETPARMVDPRPQPQIEVPANAWAPGDIKNYMTQAILVTLFCFIPTGIPAIVNA
                     SRVNGLIATGRVDEAMAASKKAHNWIISSLVINLSFWVFGIIMSIITQ"
     gene            2390..3031
                     /locus_tag="PLO35_05565"
     CDS             2390..3031
                     /locus_tag="PLO35_05565"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOH79170.1"
                     /translation="MKCPKCNEENSSTAAYCKNCGEPLRLYKTCKNGHNFDAGLENCP
                     YCPSGSQTMGGARTQIDMSSGGGDKTVIDMSPPKLSPTQPGMASAAGDKTMIFGVPVA
                     GSATSETPQAGIRKLVGWLVTYDINPAGIDFKLSVGRHKIGSNPKCDIVIAQPGISED
                     HALLLYRDEKFLLQDNLSTNGTFVNEVLIEDKLELKNDDVIRIGNIKLKLKVI"
     gene            3053..4612
                     /locus_tag="PLO35_05570"
     CDS             3053..4612
                     /locus_tag="PLO35_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH79171.1"
                     /translation="MKHFLLTTLTSITVLGLYAGAVNVKHVKSVPDNFPSMVSTVQVL
                     DSSEEPIPGLKSGNFSLAVDASQVDSIRVTSYGETGLGLNIMLCIDTSGSMRGAPIQM
                     VKEAILPFIDRVRSVDKIGISIYADDYRLLTDFTSDKELLKRVVKDIQPQGSYTALYY
                     GAYKALQHLKNSETQGGKILVLMGDGKNENPSESYNENDVIKVASEEGIPIFSIGYTK
                     VETIYLQALERMADSTGGSYYYAPNAADLKAHFERLNRQISNIYILSYLVTGVAGDGK
                     EHNLMIKASNEMGSQEARARFTAPAGRKAASASKKGGKGKLGLGLILIIVGGLIVIGA
                     GGYFLLRSAKRKRLAEEQRLQELEDQRRREVEAERQRRLELEKELQRKNMPNPDQGPP
                     TQPKVPPMSADRERTMILGPGGQTSKPGGQATQLRMEIVLGNMAGKIFTIDSSGATIG
                     RVGDNRIILNDNTVSAHHARISYEGGSFQITDLGSSNGTYINGNKISSFRITDGCSFK
                     IGAMEGTFSLL"
     gene            4621..5790
                     /locus_tag="PLO35_05575"
     CDS             4621..5790
                     /locus_tag="PLO35_05575"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOH79172.1"
                     /translation="MNKEKAPDFVIGNRSDVGSVRSENQDYYGSYSGAFGQLVIVCDG
                     MGAYEGGEIASRLAVEAISGHFARLSASYDPRTELETAFQLAQGSIREHGALHPETAG
                     MGTTAVLLLIRDGKWWVANLGDSRLYLKREGKARQVTKDHSWVQGMVDSGIITAAQAA
                     DHPKRNIITKALGTEHFIPEVQGPYTLGRGDTFLLCSDGLYHYFSLGELEKHMEADPQ
                     TACQRLVDLANQRGGDDNITVQILKSNLGEKPRPAIYKKLPMLGVLLLCLVAIGMSAY
                     WLVVYLRKPVQTEKAPVAQKEKPFQNGRPQTLVPEDKTEETKEPLSGSKPEDKARSTG
                     DKKKSPPAPVKAPVQEQTKSPGTNSSATDSNPNPGSSASVPSTGTPSTAGETSGP"
     gene            5787..6140
                     /locus_tag="PLO35_05580"
     CDS             5787..6140
                     /locus_tag="PLO35_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79173.1"
                     /translation="MKRAYIIAATLFLVLLAACDLKLSSLKREQFEVLKRNVVEILGE
                     EYKVKKIRISNEGYSDDAKNEYIVDFSFDLAKPLAFLPSSNLPGKLIFQRDSEGEWNC
                     SFNSGNPSELFNLLQ"
     gene            6137..6565
                     /locus_tag="PLO35_05585"
     CDS             6137..6565
                     /locus_tag="PLO35_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79174.1"
                     /translation="MKAFRSKSFWLLLSALLLAFLAWNAYQLTRLQKETHISAAGAIL
                     SKGEVRISIPNRSVLPFTLLPFRLEIRDKGGKHLFDAQSFSPVRIKALATTEVPLQID
                     LNARPVDLFRAASGVRVSGEVKARLLGITLKRRISQDYAL"
     gene            6555..8822
                     /locus_tag="PLO35_05590"
     CDS             6555..8822
                     /locus_tag="PLO35_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOH79175.1"
                     /translation="MRCNKCSQELEADSKFCPFCGARIMASNGSQPQIIRIGRSKSND
                     FVLDHGKVSSRHCQLSIYGDGNYVLEDLGSTNGIYVNGRKVQIQRVGLTDKVTLGRDV
                     PLDLARVIGSASRPAVREPVTPPGGIDLRAKSDITIGREEGNDIVVRNIRVSRHHARL
                     MRSGPGWELSDLGSANGTFVNGRRVTKALITERDSIVVGGVPLDLESLFRQRKQDWNA
                     DLNLLAQDLSFRVPEKIIVDDINLRMCPGQFIGLIGPSGCGKTTLMMMLNGYLRPSRG
                     SVLLNGVSLHHNLQSFQGQLGYVPQDDIIHRELTVRESLDFTSQLRLGRQIPAGERQR
                     QISHILDSLGLSATENTLIGSPEKKGISGGQRKRVNMAQELITEPQFFFLDEPTSGLD
                     PRSDREVMKLLGEISDNGHLVLLTTHNIDAVNFAILSHLIVIAPGGRLAYYGPSSEVL
                     SYFGVQNPEDIFEVLEKRDPDGLKDRYMASRYYSEYSRPPDRELAPRAAFSDTQGLRK
                     GRSDAFHQFLVLCRRAFLVKTRDVFSAAVLLLQAPIIGLFTHLVFKDAENISSLFFVL
                     VIAAIWLGCSNSAREIVTEQSIFRREQKASLSIDAYLWSKLCVLALLCLVQCLILTGF
                     AAITMDLSFFELFGVLSLVSITSLSMGLALSAVVKTGETAMALVPVALIPQVILGGLI
                     VPFGNIPEAVKALAGLMLSRWSFELLLVLHDDSLLTESIGFNMDNLGPDIAVMAAMCI
                     FFIILTRVALKRKVR"
     gene            8858..10162
                     /locus_tag="PLO35_05595"
     CDS             8858..10162
                     /locus_tag="PLO35_05595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOH79176.1"
                     /translation="MKLEPGQMIRDYQIIRSLGEGGMGEVWLAQEDLLERQVAIKRLN
                     PSLTQEAQFVQRFQNEARIQAQLTHENIVHLISFFSEEDSHYMVLEYVPGITLRDLIL
                     QTGPIPEARSLNILRQITSALVHAHSKGIVHRDIKPSNIMIDTEHGDRVKIMDFGIAR
                     LMTDGHLTRTGTKMGTIMYMSPEQVLALKDIDHRSDIYSLGVVFYEMLCGKTAYQVDT
                     DSDFLVQRAIVDTPIPDPREIYAHISDESVKVLTGMTSKDREERIGLEEVYNLITGYE
                     GANNIRPVFQIKHPKSQTKDNKAIEYDDLFWQIQKAKEQKKHTKKLPLILLATTLIEH
                     LILWFGYDEWEYIDGFLPAYIVLVCLSVLQYIATIRQIKNLPSKGALMISVGTFIVVL
                     LVAGGLCTRLSSEAQSLKPSIVFLFAQIVMASLVGFFIYHEH"
     gene            10377..11186
                     /locus_tag="PLO35_05600"
     CDS             10377..11186
                     /locus_tag="PLO35_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HOH79177.1"
                     /translation="MTINDVKSMVSPDFDFDAYSRQNGDAYWLASELMDLLGYASMQS
                     FRKVINRAMIVLNNLEIDISANIITMTSEDGTQDYKLSRFACYLCAMNADVRKKQVSQ
                     IQAYFIAFTEAFTQWVEHEQAIERVYIRGEISEHERQISGVAKQHGIQAYALFQNAGY
                     RGLYNMSLEKLKMIKGIPENRSPLDFMDTTELAANLFRITQTEERIRNQNLKGQTALE
                     RAAETVGKDVRGLMIKNGGTAPENLPTVQDIKEVRKSIKKTHKKMLGKDKK"
     gene            complement(11207..12931)
                     /locus_tag="PLO35_05605"
     CDS             complement(11207..12931)
                     /locus_tag="PLO35_05605"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH79178.1"
                     /translation="MKKYLALLSMVLLLAGLFAARKAVVIGNSTYSPYLNDLSNPVND
                     ALLLQKTLTNTGFQVSLLTDASRRQIRDLVQEVSQGLMDGDELFFYYSGHGIQVNGVN
                     YLLPIGADIQDEIDCEDEAISANWMLGKLSAAGTVIFILDACRDNPFLRTKSTVNKGL
                     AVMKTLSGKNQIIVYATEENQLAQDGSGGNSPFAEVLARHIDSSEQKFQEMLPSIKRE
                     VLAKTNNAQSPTAYGILLNDFYFREPKANDPQPQGILYTSFTTEEPYLSFRYPQGWFI
                     EESGHEISIYLRNGDEDPVIIFDLAEDLPNPDQGFAAWAKAYLIMLLEEEDMSYSIQS
                     EEGGRALGFEQFRCQMLAIEGGVSRRYDIVMLNLGGEVLLIAYIVEPHVYDRYHSGFE
                     NILQTLRIDSGYLARLQSSREDPATGYSIENYYPPWWEDRADADFLLVNGSGTSTSES
                     SSLDRAKASALFALPQELEYELKYWIKNFQEEAAINDPVLLSSSALLVSSVLSLDYSD
                     WNFGNTETRRVNIDGDIMYNSYIQLKIPRSTYLGNLCKAISRDPILYARMKKSATFLE
                     IEKAYRSK"
BASE COUNT         3219 a         3520 c         3621 g         2826 t
ORIGIN      
        1 cctgcccctc agcccgtaca agtgagaaag gaagccagcg ggatggtgta tgtggcggga
       61 ggaagctttg agatgggcaa caataccggg cgtcaggatg aaagaccggt gcacacggta
      121 actctgtcgg cgttttatat ggccaaattc cccgtcaccc aggagcaata tcaggctgta
      181 atgaagctca atccctccca gtttaagggc aacaagcacc cggttgaaaa gataagctgg
      241 agggaggcca ttcgttattg caacatgctg agcaaaattg aggggctcaa accgtgctat
      301 ttgactggtt catctggaat gtctccagat aatcccgagt gtgactggct cgccaatggc
      361 tacaggctcc ccaccgaggc tgaatgggaa tatgccgcca ggggaggcgt ttacgctcaa
      421 aaaggcggcc cggtagcgac tttggacctc agacaggaag gtggtaaaac aaacgatgcc
      481 atgcacatga cgcaagcggt gggtttggcg cctgccaatg agctgggaat aactgatatg
      541 ctgggcaatg taaatgaatg gtgctgggac tggtatatgg tggagtatta cgctcacagt
      601 attgcccgca acccgattgg gcccatgcag ggcatgtata aagtatgcag aggcgggtcc
      661 tggaagaatc tatccatgtc tgtcaccacc aggttttcct ttgaactgaa ccagttttat
      721 aacaatgtcg ggtttagagt tgtgagatcg ggatgagcgt ttctaattcg gttaaacacc
      781 agttgcagga tcccgggagg agtctgatca ggcctgtccc caattttatc gattccttcc
      841 gcttcctcag cctcctcttt ctcctcattc tgactggctt tctgctgctg ttccgcttca
      901 cggagccggg cgctttgggg ccaggtttca ggtgtccggt gcatcaatac accgggctgc
      961 tgtgtccggg ttgcggaagc atgagagcgc tgcacagcct cgtccatggc cacttcatcc
     1021 cggccctgag ggccaacttt atgatggtgc tgctcttacc ggggctgcta tatagttttg
     1081 cctatggtct gctgacgggc cgggaaccgg gatccaatcc ccgcctcaaa gcccggttga
     1141 gtaatatgtt gataattgtc ctgctggtct ttacgattgg cagaaatctc ttgggaggag
     1201 tctgatgttt taccccggcg attcgatcca gggtttcacg atcatccgcc agcttggcaa
     1261 aggcggcatg ggagaagtat atctgggtga ggatgcccat ctggcccgca aggtggcgat
     1321 caaagaactg agctccgcgc tgggctccga cgaaagcttt acgcagcgct tcaaacacga
     1381 agcccagttg caagcccggc tcagccatcc caatatcgtg tctctctaca gcttttttga
     1441 ggagggagga cgctattaca tggtgctgga atacgctgag gggatcacgc tcagagacct
     1501 gatcaggcag acgggcccgg ttccctatca aagggccaca actatcatcc ggcaggtttt
     1561 ggaagcactc gagtatgccc accggcaaaa gatcatccat cgcgacatca agccggtcaa
     1621 tatcatcgtc ggggccaatg accaggtgaa aatcctggat tttggtatct ccaagatcat
     1681 gggcgagcga ggtctcaccg gcacgggaca aatgcttgga acggtgaact atatgagccc
     1741 ggagcaggtg aaagccctca aggatatcga cggccgcagc gatctttaca gcctgggcgt
     1801 gaccttcttt gaggtcctga gcggcaggct gccctacgat accctgacgg acagcgacta
     1861 tgagatcatg aaccagatcg tccaggcccc catgccggac ccgaggagct actatcccgg
     1921 cattccggaa aagacggtgg ccatcctctt caagatgctg gccaaagaca ggagtcagcg
     1981 ctatagctcg gcctgggaag tgatgcagga ctttgaggcc ccggtggtta gcatgccggt
     2041 ccaggctgaa actcccgcac gaatggtcga tccccgtccc caacctcaga tcgaggtccc
     2101 tgccaatgcc tgggccccgg gggatatcaa aaactatatg acccaggcga tcctggtgac
     2161 cctcttctgc tttattccca ccggaattcc tgccatagtc aacgcgtcca gggtaaacgg
     2221 tttgattgca acgggaaggg ttgacgaggc catggcggct tccaaaaaag cccataattg
     2281 gatcatcagt tctttggtaa tcaatctcag cttctgggtc tttggtatca tcatgtcaat
     2341 catcacacaa tgacaagcct attagatatt tacaattaaa ggagttacca tgaagtgccc
     2401 caagtgtaac gaggaaaaca gcagcacggc cgcctattgc aaaaactgcg gagaaccgct
     2461 gcgcctctat aagacctgca aaaacggcca caactttgac gcgggcctgg agaactgtcc
     2521 ctactgccca tccggttcac agaccatggg aggggcccgc acccagatcg acatgagctc
     2581 cggaggggga gacaagaccg tcatcgacat gagccctccc aagctgagtc ccacccagcc
     2641 tggcatggcc agcgcggccg gagacaagac catgatcttt ggagtgccgg tggccggttc
     2701 cgctacctcc gagactcctc aggccgggat ccgtaagctg gtgggctggt tggtgaccta
     2761 tgacatcaac cccgccggga tcgatttcaa gctgtctgtg ggacgtcaca agatcggcag
     2821 caatcccaag tgcgacatcg tgatagccca gccagggata tcggaagacc acgccctgct
     2881 gctgtatcgg gacgagaagt ttctgctgca ggacaatctc tccaccaacg gcaccttcgt
     2941 gaacgaggtc ctgatcgaag acaagctgga acttaaaaac gacgatgtga tccggatcgg
     3001 caacatcaag ctcaagctga aagtaatcta agcctttggg cagggagcag atatgaaaca
     3061 ctttctattg acgaccctga cctcgataac ggtcctgggc ctatatgctg gcgcggtaaa
     3121 cgtcaagcac gtaaaatccg tgccggataa cttccccagc atggtgagca ccgtgcaggt
     3181 gctggatagc tcggaggagc ccatcccggg cctgaagagc ggaaacttca gcctggccgt
     3241 cgatgccagc caggtggatt ccatccgggt gaccagctat ggcgagacgg gtctgggact
     3301 aaacatcatg ctctgcatcg atacatcggg gtcgatgcgc ggcgcgccca tccagatggt
     3361 caaggaggcg atcctgcctt tcatagacag ggtgaggagc gtggacaaga tcggcatctc
     3421 gatctacgcg gacgactatc ggctgctgac ggacttcact tccgataagg aactgctcaa
     3481 acgggtggtg aaagacatcc agcctcaggg cagttacacg gcgctctatt acggggccta
     3541 caaagccttg cagcatttga agaacagcga gacccagggc ggcaagatcc tggtgctgat
     3601 gggcgacggc aaaaacgaga atccctccga atcctacaac gaaaacgacg tgatcaaagt
     3661 ggcctccgag gaaggcatcc ccatcttcag catcggctac accaaggtgg agaccatcta
     3721 cctgcaggcc ctggaacgca tggcagacag cacgggcggc agctattact acgctcccaa
     3781 cgcggcggac ctcaaggccc actttgagag gctaaaccgc cagatcagca acatctatat
     3841 cctcagctac ctggtgacag gtgtggcggg agacggtaaa gagcataacc tgatgatcaa
     3901 ggccagcaat gaaatgggca gccaggaagc cagggccagg ttcaccgctc cggctggcag
     3961 aaaagcggcc agcgcgtcta aaaaaggcgg aaaaggcaag ctgggcctgg gattgatctt
     4021 gatcatcgtc gggggcttga ttgtgatcgg cgcgggaggg tatttcctcc tgagaagcgc
     4081 caaacgcaaa cgcctggccg aagaacaacg cctgcaggaa cttgaggacc aaagacgccg
     4141 ggaagtggaa gcggaacggc agcgccgtct ggaattggag aaagagctgc agcgcaagaa
     4201 tatgcccaat cccgaccagg ggcctcccac ccagccaaaa gtcccaccca tgagcgcgga
     4261 ccgcgaacgc accatgatcc tcggccccgg aggccagaca tccaaaccgg gaggacaggc
     4321 tactcaactg aggatggaga tcgtgctggg caatatggcc ggcaagattt tcacgatcga
     4381 ctcatccggg gccacgatcg gccgagtggg agacaaccgc atcatattga acgacaacac
     4441 ggtatcggct catcacgcca ggatatccta cgagggcggc agcttccaga tcacggacct
     4501 gggctcttca aacggaacct acatcaacgg caacaagatc tccagcttcc ggatcaccga
     4561 tggctgcagc tttaagatcg gcgccatgga aggaactttc agcctgctat aggagggcgc
     4621 atgaataagg aaaaagcccc tgatttcgtg atcggcaacc gctccgacgt gggcagcgta
     4681 agaagcgaga atcaggatta ttacggcagc tacagcggag cgtttggcca gctggtgatc
     4741 gtctgcgacg gcatgggagc ctatgaaggg ggtgagatag cctccaggct ggccgtggaa
     4801 gccatttccg gccactttgc caggctgagt gcttcctatg accccaggac cgagctcgag
     4861 acggccttcc aactggccca gggcagcatt cgtgaacacg gcgccctcca tcccgaaacc
     4921 gctggcatgg gtactaccgc ggtgctgcta ttgatccggg acggcaagtg gtgggtggcc
     4981 aacctgggag acagccgtct ctatctgaag cgggaaggca aagcccgtca ggtgaccaaa
     5041 gaccactcct gggtgcaggg tatggtggat agtggcatca tcactgccgc gcaggcagcc
     5101 gatcatccca agcgcaatat catcaccaaa gcgctgggca cggagcactt tatcccagag
     5161 gtccagggcc cctacacgct gggcagggga gatactttcc tgctttgcag cgacgggctc
     5221 taccactatt tcagcctcgg cgaactggaa aagcacatgg aagccgaccc ccagacagcc
     5281 tgccagcgtt tggtggacct ggccaatcag cgcggcgggg acgacaatat caccgtccag
     5341 atcctcaaat ccaacctggg agaaaagccc cggcccgcga tctataagaa gctgcccatg
     5401 ctgggagtgc tgctgctgtg cctggtcgcg atcgggatgt cggcctactg gctggtggtg
     5461 tatctcagaa agccggtaca gacagagaaa gctcccgtcg ctcaaaaaga gaagccattc
     5521 cagaacggca gacctcagac cctggtgcct gaggacaaga cagaagagac aaaagaaccg
     5581 cttagcggct caaagcctga agataaagcc aggagcacag gagacaaaaa gaaaagcccc
     5641 ccagctcctg tcaaagctcc tgtacaagag cagactaaga gcccgggaac caattcctcg
     5701 gccacggata gtaacccgaa tccaggaagc tctgcttcag tcccatctac cgggacccca
     5761 tcaaccgcag gagaaacctc cggcccatga aacgtgcgta tatcatagcg gctactctgt
     5821 tcctggtgct cctggcggcc tgcgatctga agctctccag cctcaagcgc gagcagtttg
     5881 aagtcctcaa gcgcaacgtg gtggagattc tgggcgagga atacaaggta aagaagatca
     5941 ggataagcaa cgaaggctac agcgacgacg ccaaaaacga atacatcgtg gatttcagct
     6001 ttgacctggc caaacctctg gcctttctgc ccagcagcaa cctgcccggc aagctgatct
     6061 tccagcgcga ttccgaaggg gagtggaact gcagcttcaa ctccggcaac cccagcgagc
     6121 tctttaacct cttgcaatga aagcgttccg ttccaagtcc ttttggctgc tcctgagtgc
     6181 gttgctgctg gccttcctgg cctggaatgc ctatcaactg acgcggctgc aaaaggagac
     6241 acacatcagc gcggccggcg ctatcctcag caaaggcgag gtcaggatca gcatccccaa
     6301 ccgctccgtg ctgccattta ccctgttgcc cttccggctt gagatcagag acaaaggcgg
     6361 caaacatctc tttgacgccc agagctttag tccagtccga atcaaagccc tggccaccac
     6421 cgaagttccg ctgcagatcg acctcaacgc caggcctgtg gacctcttca gggcagcttc
     6481 aggagtcaga gtaagcggag aggtgaaagc cagactgctg ggcatcactc tcaaacgaag
     6541 gatcagccag gattatgcgc tgtaacaagt gttcccagga gcttgaggcg gattccaagt
     6601 tctgcccctt ctgcggtgcc aggatcatgg cctccaatgg atcccagccc cagatcatca
     6661 ggatcggccg gtccaaaagc aatgacttcg tactcgacca tggcaaggtc tcgtcccgtc
     6721 attgccagct aagcatttac ggcgatggca actacgtgct ggaagacctg ggctccacca
     6781 acggcatcta tgtcaacggt cgcaaagtgc aaatccagcg ggtggggctg acggacaagg
     6841 tgaccctggg ccgggatgta cctctggatt tggccagagt gataggatcg gcatccagac
     6901 cggcggtacg cgagcccgtt acccctccgg gagggataga tctgagggct aaaagcgaca
     6961 tcacgatcgg cagggaagag ggtaacgaca tcgtggtgcg caacatccgc gtctcccgtc
     7021 atcatgccag gctgatgagg tctggtccgg gctgggagct cagcgatctg ggcagcgcca
     7081 acggcacctt tgtcaatggc cgccgggtga ccaaagccct gatcactgaa cgagacagca
     7141 ttgtggtggg cggcgtgccg ctggacctgg aaagcctgtt ccggcagcgc aagcaggatt
     7201 ggaacgccga tctgaacctt ttggcccagg acctcagctt cagagtgccg gaaaagatca
     7261 tcgtggacga catcaacctc aggatgtgcc cgggccagtt cattggtctg atcggccctt
     7321 ccggttgcgg caagaccact ctgatgatga tgctgaacgg ctatctgagg cccagccgtg
     7381 gatcggtgct cctcaatgga gtctcgctgc atcacaacct ccagtccttt cagggtcagc
     7441 tgggctatgt gcctcaggac gacatcatcc atcgcgagct cacggtgcgc gaatcgctgg
     7501 atttcaccag ccagctcagg ctgggccggc aaatcccggc cggggaacgt cagcgccaga
     7561 tcagtcacat cctggattct ttgggtctga gcgccacgga aaacaccctg atcggctctc
     7621 ccgaaaagaa aggcatcagt ggaggccagc gcaaacgcgt caacatggcc caggaactga
     7681 tcacggagcc gcagttcttc tttctggacg agcccaccag cggactggac ccacgcagcg
     7741 accgggaagt gatgaagctc ctgggtgaga tttccgacaa cggacacctg gtgctgctca
     7801 ccactcacaa catcgacgcg gtcaactttg ccatcctcag ccacctcatc gtcatcgctc
     7861 cgggaggcag actggcttac tatggtccct cctcggaagt gttgagctat tttggggtcc
     7921 agaatcccga ggatatcttt gaagtgctgg aaaagcgtga tccggacggg ctaaaggaca
     7981 gatacatggc cagccgctat tacagcgagt attccagacc gccggacaga gagctggccc
     8041 caagggccgc cttctcagac actcagggcc ttcgcaaggg acgctccgac gcttttcacc
     8101 agttcctggt gctctgcaga cgcgccttcc tcgtgaaaac cagagacgtg ttcagcgccg
     8161 cggtattatt gctccaggcc cctatcatag ggctttttac ccatttggtc tttaaagacg
     8221 cggaaaacat ctcctccctg ttttttgtgc tggtgattgc ggctatctgg ctgggctgca
     8281 gcaattccgc ccgcgagatc gtgacggagc agagcatctt tcgcagagaa cagaaggcca
     8341 gcctcagcat cgatgcctac ctctggtcca agctctgcgt cctggccctt ctctgtctgg
     8401 tgcagtgcct catcctgaca ggcttcgcgg ccatcaccat ggatctgagc ttctttgagc
     8461 tcttcggtgt gctcagcctg gtgtccatca cctcgctcag catgggtttg gcactttctg
     8521 cagtggtgaa gactggcgag acggccatgg cgctggtgcc ggtggccctc attcctcagg
     8581 tcatcctggg agggctgatc gtcccctttg gcaatatccc ggaggcggtc aaagcgctgg
     8641 ctggactgat gctgagccgt tggtccttcg agctgctgct ggtgttgcat gacgactctc
     8701 ttctgaccga aagcatcggc ttcaatatgg acaacctggg cccagacatc gcagtcatgg
     8761 cagcgatgtg catattcttc ataatcttaa cccgcgtggc gctgaaacgc aaggtccgct
     8821 gaaactatgt atgtatcatc tctaagggag gcaaaacgtg aaacttgaac ccggacaaat
     8881 gatcagagac taccagatca tccgttccct cggcgagggt ggaatggggg aagtatggct
     8941 ggcccaggaa gacctgttgg agcggcaggt ggctatcaag cgcctcaatc cgtctctcac
     9001 acaagaggct cagtttgttc agagattcca aaatgaagct cgtattcagg cccaactcac
     9061 gcatgaaaat atcgtgcacc tgatctcttt ctttagtgag gaggactctc actacatggt
     9121 gctggaatat gtgccaggta tcacgctgag ggatttgatc ttacagacag ggccgatacc
     9181 agaggcaagg tccctgaaca tcctcaggca gataacctcc gctttggtcc acgctcattc
     9241 caagggcatc gtccatcgcg atatcaagcc ttccaacatc atgatcgata ctgagcacgg
     9301 agaccgggta aagatcatgg acttcggcat cgccagactg atgacggatg gccatctcac
     9361 tcgcaccggg accaagatgg gcaccatcat gtatatgagc cctgaacagg tgctggccct
     9421 caaagacatc gaccaccgca gcgatatcta ttcgctgggc gtggtatttt acgagatgct
     9481 ctgcggtaaa accgcctatc aggtggatac ggacagcgac tttctggtgc agagggcgat
     9541 agtggataca ccaattcccg atccgaggga gatctatgcc catatctctg atgagtctgt
     9601 taaagtactg acggggatga ccagcaaaga cagagaagaa agaataggac tcgaagaagt
     9661 gtacaacttg ataacaggtt atgagggcgc caataacatt agaccagtat ttcagattaa
     9721 acaccccaaa tcccaaacaa aagataacaa ggcgatcgaa tatgacgacc tgttttggca
     9781 gatacagaaa gctaaggagc aaaaaaaaca cacgaaaaaa ctgccgttga tacttcttgc
     9841 cacgacgctt atcgaacacc tgatcctttg gtttggctat gatgagtggg aatacataga
     9901 cgggtttctg cctgcgtaca ttgttcttgt ttgcctgtct gtgcttcagt acatcgctac
     9961 aatcaggcaa atcaaaaatc tcccatcaaa aggcgcatta atgatatctg tgggcacttt
    10021 tattgttgta ttattagtgg ccggagggtt atgtaccaga ttgagtagtg aagctcagtc
    10081 attaaaacca tctattgtgt ttctattcgc ccagattgta atggcatcgc tagtcggttt
    10141 ttttatatac catgaacatt agccagtata tttttgatga tccctttaac tcatatagaa
    10201 aactaagatc cattagttat tttgtaattc ttttttcacc aattttggct gctatcctgc
    10261 tgctcattcc tgatgtataa ttctgctaag ctctcttcta agctttataa agtaggaatt
    10321 gacaaacaaa gaccctgcaa tttaatgtct tctaaacata tgatggaggt atcccaatga
    10381 caataaatga cgtaaaatcc atggttagcc cggattttga ttttgacgct tactcacgtc
    10441 agaatggaga tgcctactgg ctggcttcgg aactaatgga ccttttgggg tatgcctcca
    10501 tgcaaagctt ccgcaaggtg atcaatcggg ctatgatagt cttgaacaac cttgagatag
    10561 acattagcgc caatatcatc acaatgacat ccgaggatgg gacccaggac tacaagttat
    10621 cacgatttgc gtgttatctt tgcgccatga acgcggacgt cagaaagaag caggtttctc
    10681 agatccaggc ctatttcatc gccttcacgg aggcttttac acaatgggtt gaacatgaac
    10741 aggccatcga aagagtctat atcaggggcg agatttctga gcatgagcgc cagatttctg
    10801 gtgtcgccaa gcaacacggc attcaagcct acgccctttt tcaaaacgcg ggttatcggg
    10861 gtctttataa catgagcctg gaaaagctga aaatgatcaa gggaatccct gaaaaccgca
    10921 gtccattgga ttttatggac accactgaat tggctgccaa cctgtttaga atcacccaga
    10981 cagaggaaag gatcagaaat caaaacctca aagggcagac tgcattggaa agagcggcag
    11041 agacagtcgg taaagatgtc cgcggactga tgattaaaaa cggaggtacg gccccagaaa
    11101 accttccaac cgtgcaggac ataaaggaag tgagaaaaag catcaagaag acccacaaaa
    11161 agatgctggg taaagacaaa aagtaaaccg tctttctggt caattctcac ttggatctat
    11221 aggccttctc gatctccaga aaggtggcgg atttcttcat gcgggcatag agtatggggt
    11281 ctctggagat ggctttgcag aggttgccga ggtaggtgga gcgcgggatt ttcagttgga
    11341 tatagctgtt atacatgatg tccccgtcta tgttgacgcg cctggtttcg gtattgccga
    11401 agttccagtc gctgtaatca aggctgagga cggagctcac cagcaaggcc gatgaactca
    11461 gcaagacggg atcgttgatg gcagcttctt cctggaagtt ttttatccag tatttgagct
    11521 catactccag ttcctgaggc agagcaaaga gagcggaggc cttggcacga tccaaagaac
    11581 tgctttctga agtggaagta ccactgccgt tgacgagcag gaagtccgcg tcggcccggt
    11641 cttcccacca ggggggataa tagttttcga tagaataacc cgttgcagga tcttccctgg
    11701 aagactgcaa gcgagccagg taaccggaat cgatcctcag agtctggagg atattctcaa
    11761 agccggaatg atagcggtca tagacatgag gttcaacaat gtaggctatc aagagcactt
    11821 caccacccag gttgagcatc acgatatcat agcggcggga tactcctcct tcgatggcca
    11881 gcatctggca gcggaattgc tcaaaaccca aggcccggcc tccttcttca ctttggatgg
    11941 agtagctcat atcttcttct tccagcagca tgatcagata ggctttggcc caggcggcga
    12001 agccttgatc cggattggga agatcttccg ccaggtcgaa gatgatgacc ggatcctcgt
    12061 caccgttcct gaggtaaatg ctgatctcat gcccggattc ctcgatgaac cagccctggg
    12121 gataccgaaa gctgaggtag ggctcctcag ttgtgaagct ggtataaagg atgccctggg
    12181 gctgggggtc attggccttt ggttctctga aataaaagtc gttcagaagg ataccgtagg
    12241 cggtggggct ttgggcattg ttggtcttgg ccagcacctc tcttttgatg gatggcagca
    12301 tttcctgaaa cttctgctcc gaactatcga tatgccgggc cagcacttcc gcgaaaggac
    12361 tgttaccacc actgccatcc tgggccagct gattttcctc ggtggcatag acgatgatct
    12421 ggtttttgcc ggatagggtc ttcatcaccg ccaagccttt attgacggtg cttttggtgc
    12481 gcaggaaggg attatcccgg caggcgtcca ggatgaagat caccgtcccg gcagctgata
    12541 gctttcccag catccagttg gccgagatag cctcgtcctc gcagtcaatc tcatcctgaa
    12601 tgtccgctcc gatgggcaac aggtagttca ctccgttcac ctggatgccg tgaccgctgt
    12661 aatagaaaaa cagctcgtcc ccatccatga ggccttggga tacttcctgg accagatcgc
    12721 ggatttgtct tctggaagcg tctgtgagca gcgaaacctg gaagcctgta ttggtgaggg
    12781 ttttctgcag caggagggcg tcgttgacgg ggttgctgag gtcattgagg taagggctgt
    12841 aggtggaatt gccgatcacc acagctttgc gggcggcgaa aagccctgcc agcagcagga
    12901 ccatgctcag cagcgcgaga tacttcttca tgttttatgg cgctccctaa agggtttttg
    12961 gctgctcttc cggtatgagg catccttatc ctcctgatgg aatggatcat gatgtaagat
    13021 atctataaac ataccgttta gcatattctc accagtgagc gggaacggaa caatcggtca
    13081 agcaaaatat ccgcaaccca ggctggattt tttgtccttg actgaaatat aaggtttcat
    13141 taagcttgcg ccatcaatgc tgggagtgtt cagatggtta ttgatc
//
LOCUS       DAOSFN010000066        13119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_3974_length_13119_cov_10.125842, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000066 DAOSFN010000000
VERSION     DAOSFN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13119
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3974_length_13119_cov_10.125842"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLO35_05610"
     CDS             <1..309
                     /locus_tag="PLO35_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79179.1"
                     /translation="GISPTEGISPTEGISPTEVISPTEVISPTEVISPTEVISPTEGI
                     SPTEGISPTEGISQRSSHIPIPDAFGKKVIDNIGRFNADAIKLLNDYNLTQEIAYEVI
                     "
     gene            296..3190
                     /locus_tag="PLO35_05615"
     CDS             296..3190
                     /locus_tag="PLO35_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79180.1"
                     /translation="MRLSNRLLKMFLLLGLLAAAGLIIAQKETASKQNVVEDLTVEDL
                     PADDGSGLILSWKPLHRSKRIIEYRIYRGIDPDTLFFLQSVQVNVNTGVAADRMYYYD
                     SGGGDFIDLSSPGKLRKEKQQDDKSPLYRNVPRDMELAARLSEKFDIISYVERPAFYK
                     HGRKVWSADPADSTAYAGFKFKHQNLQAGLRAKETYYYTVVAVNERNQFQDYAKPVAG
                     TPVGNPPDPATALYGSWLADTKEYMFEWDYPLVKDDILQYQIYRVKDMPDSLWARLKT
                     LPDTLGIYRQPLGDPGPVQGGQLPNYTRFAMPEMSSEEVASYRYAIELADMEGSSLSD
                     LVTIQIADSKALPAKPSFYTEDKPNDKGDRLTVIWDKPITFVTKTTSLNAKNTRLKIN
                     YQLNKAETQKVTNIYFSLYIPGQSKPFVTINEFYQDLSFKIKVPAGYDYRKGFRVRIT
                     MKGKDIDPRNYILEQDLEWDTAMMALMPGKNLWRNGEEVSRVQNVVYRRGIRSPNYYL
                     VKRNTSFDSNLDVNISYPTMVPRLVSGFNFVKGNTLHTYMNGKRFSRPLKAGESKAGY
                     ALVSPVIDFTFDKDKEALINTSIFKDEALKSMDLLKKEVGDLKAQVAGLPANADSTLG
                     LELKAKLEAQEKKLALFTENKHMQAALKRGSDRSWIKYIAGVREPESRNHSYQIVKTN
                     GRGLFTVSDPDTSATGEVNFYYPISNWFAKEKLITLFAVLLYGALVVSFVTLAKRGKD
                     LYIRPIAGLDEIDNAVGRATEMGRPMLYCMGSGSLSDVSTLASMGILTQVAKRAAEYD
                     TKLIVPCYDYIVMPVVQEIVREAHYSVGRPDTYDKNNIFYLTNSQFAYVAGVNGIMIR
                     EKMATNFFLGFFAAEALLMTETGNTVGAVQIAGSDAITQIPFFITTCDYTLIGEELYA
                     ASAYLNREPMLLGTLKAQDYLKIIIVFLVLVGTILSTLQVTNLLNILPTK"
     gene            3204..3833
                     /locus_tag="PLO35_05620"
     CDS             3204..3833
                     /locus_tag="PLO35_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79181.1"
                     /translation="MKKTVPLIIVIVGGIVFLLWVFIPHKIMADDFYYQFYTPWSRAM
                     APFAMLLGVMSLSMMHNAKIKRRAPKWQFSYFFFAGFIVTSLAGFIGGTQKGGLYMWM
                     FETMAMPMSATMFSLLAFYMASAAYKAFRARSVEATVLLVAAIVVMLAQVPLGVKISK
                     HLPTISQWILDVPNLASKRGIMLGVGLGSVATTLKIMLGIERSYLGGGD"
     gene            3847..5034
                     /locus_tag="PLO35_05625"
     CDS             3847..5034
                     /locus_tag="PLO35_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79182.1"
                     /translation="MNIFERIQKLDRRWIYIVVALAIVIPLAIPFNSDNVTSPSTENL
                     YQFIDSYAGRQDRAILLSFYHDASTMPELFPMEVAILRHCFERNVKVFMLTWFPQGAP
                     IIDYAINTVKEEYPNIKSGVDYCNFGYIPAALAMPTILGMGDNIAAAVKTDAEGRKLE
                     NLEIIKGVNNYSEMNLVVEFSGSSAGVTWLYYARPKFGLNIGVGVTAVMAADEYPFLQ
                     SGQKIGMLAGLKGAAEYEKLVDVFAAYREPGSPITVTVDEDGNKIVPGRAFSSDILRE
                     ESTKQLINITTQTVAKFSADEYSKFVGKYPDKAPIFDKLKTEKNGQVEIDVTKVTDDQ
                     KEALGYTAMADLNRMTRNIIYKFKVARIGMNAQSVAHIMIIVFIVLGNIGYFIQKARQ
                     ARQ"
     gene            5045..5647
                     /locus_tag="PLO35_05630"
     CDS             5045..5647
                     /locus_tag="PLO35_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79183.1"
                     /translation="MTFELFSNLVGAFFTLSIFSFLYKDNPFYQMSEQLLVGISLGYG
                     LVLTYERVFIPYLWQPIVLKHQFILIIPALLGAFYLFRFSRKLSWLSRYPIAFSMMYV
                     GASVALAMHSYILVQMRQAMVPIENVNLLLIFIGTVSVLLYFFFSKAHTGAYGRFVAV
                     GKWYMMVGFGASFGLTVMARISLLIGRIQFLVNDVFGKIF"
     gene            5741..8575
                     /locus_tag="PLO35_05635"
     CDS             5741..8575
                     /locus_tag="PLO35_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79184.1"
                     /translation="MKKAAIVTAMALLGILMLFSGSGAGFESGGLIEGLVAMDAPNDS
                     GQGVILKWKPLDKSHRIIQYKIYRGASPDSLFYFNNLDVDPVLGVIGNEITFSDQDYQ
                     PLFEFETAPNKLKKERHQPSGSPLYRAVPRDPKVIGSLIPHFEVLGAINHRVFNHHSR
                     RIDSADGSMAGYKLNQFDFIYANPLPGKTYYYSVVAVNERGKHLPGAKVVSATPVDNR
                     PSPDAELAATWVEDKAEIGFEWSMPEMGYDLQEYSGWLLPKDQAPLFKAQQELNSSAP
                     DSLFNAQWKASAIPMFQVAADPNVSTLYQKVNLKEAGIQLSRPATEYLPLLTYSDTSG
                     FQNASLADTLYVKNSSQYPPLPAFSVHNKKNNKGDNLLISVGKPVAYITQASYTSGSK
                     TKLKFNYDLLENELYPIERLRFTFTDTHGKELATITEYYPDKILYLKVPQGFKGTKKF
                     MVSTRVMLRSKKAYVEPAATQDIVFDDATRRYVGKNLSLHGHRLDKVYLDVLTRKNLD
                     QVFKPGMRSNGMLRAQDHTISFPDVLYKSVTGYDAKTRSLTIDPTISVAMNAEKGFAF
                     QASLYRSDFEKDLKEMSAKADSLRGVVKQLAAQGDTLSQAYLAAKEEGDYYSGSYGFI
                     TAHPAYKQAQKARSESAWRRILLAESVRNNRSFSYQFLLTDGHGFFQQTDTYNRDGET
                     VFTPKPVWFNTTMWATLFGSIIFGILIVYALFQARRKDLYIRPIAGLEDLDNAVGRAT
                     EMGRPVMFVPGWGTLGEPCTISFLMILSQIAKKTAEFDVRLISPHVDYFVVPIAQEIV
                     QTSYSEVGRPDAFNREDIFFISDSQFAFAAGVNGITIRERVATIMYMGYFNAEALLMT
                     ETGNQTGAIQIAGTDAITQVPFFITTCDYTLIGEEFYAASAYLSKNVELVSMLKGQDY
                     FKMVMITLIVIGSILSTFHWHGLLHFLPFE"
     gene            8580..9209
                     /locus_tag="PLO35_05640"
     CDS             8580..9209
                     /locus_tag="PLO35_05640"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOH79185.1"
                     /translation="MEKITIPTSTLKRLPQYLEVLKQLKRAGLKSVTATKISVFADIH
                     QTLVRKDLSFAGVTNIPQSGYKIRDLAAAIEKLLNWNDTSSSYLVGVGHLGSALLGYQ
                     EFSQKGLAIMAAFDNDPDRIGSVVHGVPVHSVLDFTAMATRNNIRIGIITVPPEAAQL
                     IADIMVHSGIKAIWNFTPQKIQVPPDIIVEYVDMFASLAVLSRRLAEKG"
     gene            9213..10622
                     /locus_tag="PLO35_05645"
     CDS             9213..10622
                     /locus_tag="PLO35_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH79186.1"
                     /translation="MPLKVLLVSPKATTGGLEGMRKGHQIHQGLLFVAAATRDAGHRP
                     VVVSADSISIHRFIRRYKPDVVGFSCVTATYPMTRDLIKLVKKAYPSITTVIGGHHVT
                     FMYKETIEESGVNYVCRGEGEEVFPALLNSLEVGERYPAIPGMVFKKQGKFHNDEIIA
                     LVEDINTLPRITMDLVAPDFSFTPKIVSSRGCPFFCSYCSISAFYGGKYRQRRVEDVI
                     KDIEDYISWGYNHFWFHDDNLTVDAKWVNSFCELVEEKQLKFTWNSMSRVDLICRDPV
                     LISRMARCGCRMLSIGIESGVPEVLESIHKKINLDQIHMAVKIMQSVGISYDWFMLLG
                     SGDQFDTPGIIEKNIQFFSKLPGMILVSILTPFPGTEVFERLRKEDRIRHYNWEDYDI
                     THCVYNPLGMSYKQMEAYLPIAYRRIYLAKGLGLVPLFIHGVRTKAIRLDMVTSGIKS
                     LFQTFVLKKDFYSSIRKTH"
     gene            10946..>13119
                     /locus_tag="PLO35_05650"
     CDS             10946..>13119
                     /locus_tag="PLO35_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79187.1"
                     /translation="MKKTLLLVAAVLLVCTLLQADIMPPGQIKPTLESPRGISMPGTR
                     EVPAYTFTRVPNSIIVNYYDYMIGAYSSLPLRVIPSSAGGGYFITYHGRRQATSTRRV
                     FYTYMNPQGDVISNNEITSVQNHEGFPSMVIDPVSGKPLYAWHANADADANLETQFVS
                     DAFMAGFAGLWNDIQLTADAPTSITAPNGTTTTDNEFIWPTMTIGPSPIAGKRRVYIS
                     QRNFISHAANGSPSENVYIAYTDFNGDDIESGVPLVWNHTTLPEYDAWNVDTAEFRRP
                     TSSITADMSGNIYYIGYHAAMDLDNNSLDEPDLDVFICPNYGEGTWSRMTRYSKLPAW
                     NPNSAPSDTTGYYTDPDNGDIPYGDNEIFFQVANSSHMNSVVDQRGRVHTAALWALSS
                     YNDYYYPALQFVKHMVYNPDSNQIQVNDLYPQKDPSDTFNQTFMPWDTEAPFGEVDEY
                     GDDGAGGYYPLMVTDWPFPHWDATAHTDAMMFHYNGVRISEANDHGMMAAVWQDSQRA
                     RMFNYYSDTDYSQFANTPEIYISVSPNNGDTWSEPIVLNNVETPELAGRKPMYVYPAD
                     KVIFTGMQGNQEVGKLGILFYDDYTWGSNVNAPAYHPTPDGGEVVFMELQIVFPYGVD
                     SDDPVVTPAPSILHPNFPNPFNPSTTISFDMPKSGHARLSVYNVKGQLVRALLNEQRG
                     FGSHSVVWDGTDANGNTVPSGLYFYRLNTDNHSETRKMMLMK"
BASE COUNT         3176 a         3781 c         3232 g         2930 t
ORIGIN      
        1 ggtatctcac ctaccgaagg tatctcacct accgaaggta tctcacctac cgaagttatc
       61 tcacctaccg aagttatctc acctaccgaa gttatctcac ctaccgaagt tatctcacct
      121 accgaaggta tctcacctac cgaaggtatc tcacctaccg aaggtatctc acagcgcagc
      181 tctcacattc ccatccctga cgccttcggg aaaaaagtga ttgacaatat agggcggttt
      241 aatgccgatg ccataaagct tttaaatgat tataatctaa cccaggagat agcgtatgag
      301 gttatctaac cgattgttga agatgttttt actgctcggt ttgcttgcag ccgcggggct
      361 gatcatcgcg cagaaagaga ctgcttcaaa acagaatgtg gtagaggatc ttacggtaga
      421 agatcttccc gctgacgatg gcagcgggtt gatcctgagc tggaagcccc tgcaccgctc
      481 caaacgtatt atcgaatatc gcatctatcg cgggatcgat ccggacaccc tgtttttcct
      541 gcaatccgtg caggtaaacg tgaacacagg tgtggcagcg gacaggatgt attattatga
      601 cagcggtggg ggagatttta tcgatctcag ttcccccggc aaactgcgca aggaaaagca
      661 gcaggatgac aagagcccgc tctatcgcaa tgtcccgcgg gacatggagc tggccgctcg
      721 tctgagtgaa aagtttgaca tcatctccta tgtggagcgt cccgcttttt acaaacacgg
      781 ccgcaaagtc tggtctgccg atccggctga ttcgactgcc tatgccggct ttaagttcaa
      841 acaccagaat ctccaggccg gtctgcgcgc caaagagacc tattactaca ccgtggtggc
      901 cgtcaacgaa cgcaaccagt tccaggatta tgccaaaccc gtggccggaa ctccggtggg
      961 caatcctccc gatccggcaa ccgcgctgta tggaagctgg ctggcagaca ccaaggagta
     1021 tatgtttgag tgggattatc ccctcgtcaa ggatgatatt ctacagtatc agatctatcg
     1081 ggtcaaagat atgccagaca gcctgtgggc caggctcaag accctgccgg acaccctggg
     1141 catctatcgt caaccgcttg gagatcctgg acccgtgcaa ggtgggcagc ttcccaatta
     1201 caccagattt gccatgccgg aaatgagcag tgaagaggtg gcatcatacc gttatgccat
     1261 tgaactggca gacatggaag gttcctcgct ttctgatctg gtgacaattc agatcgcgga
     1321 cagcaaagca ttgccggcca agcccagttt ctatacggaa gacaaaccca acgacaaggg
     1381 cgaccgcctc accgtgatct gggacaaacc catcaccttt gtgaccaaga ctacctcgct
     1441 aaacgcgaaa aacaccaggc tcaagatcaa ctatcagctc aacaaggccg agacccagaa
     1501 ggtgaccaac atctatttca gcctctacat ccctggccag agcaagcctt tcgttacgat
     1561 caacgagttt tatcaggacc tttctttcaa gatcaaagtg ccggccggtt atgattaccg
     1621 caagggtttc cgggtccgga tcacgatgaa aggaaaggac atagacccca gaaactatat
     1681 cctggagcaa gacctcgagt gggataccgc catgatggcc ctcatgccag gaaaaaatct
     1741 gtggcgcaac ggcgaagagg tatcccgcgt ccaaaacgtg gtataccgcc gcggtatcag
     1801 gtctccgaac tattatctgg tcaaacgcaa cacctccttt gacagcaacc tggacgtcaa
     1861 tatctcctat cccactatgg ttccgcgtct ggtgtcgggc tttaactttg tgaaaggcaa
     1921 cacgctccac acctacatga acggcaagcg cttctcgcgt ccgctcaagg ctggagaaag
     1981 caaggctggc tacgctctgg tgtcgccagt tatagacttc acctttgaca aagataaaga
     2041 ggctttgatc aacaccagta tctttaagga cgaagccctc aagagcatgg acctgctcaa
     2101 aaaagaggtg ggcgatctca aggcccaggt agctggcttg cctgccaatg ctgattccac
     2161 ccttgggttg gaactgaagg ccaagctgga agctcaggaa aagaagctgg ccctctttac
     2221 ggaaaacaaa cacatgcagg cagctctcaa gcgtggcagc gaccgttcct ggatcaagta
     2281 tatcgcgggt gtccgtgagc ccgaaagccg caatcacagc taccagatcg tcaagaccaa
     2341 cggccggggc ctctttaccg tctctgatcc agataccagc gctaccggag aggtcaactt
     2401 ctattatccg atctcaaact ggtttgccaa agagaaactc atcaccctgt tcgcggtgct
     2461 gctgtatggc gccttggtgg tgagcttcgt gactttggcc aagcgcggca aagatctcta
     2521 tatccgtcct attgccggat tggatgagat cgataatgcc gtgggccgcg ccactgaaat
     2581 gggccgtccg atgctctact gcatgggcag cggatcactt tcggacgtat ccaccctggc
     2641 atcaatgggc attctgaccc aggtggccaa acgcgccgcg gaatatgaca ccaaattgat
     2701 cgtgccttgt tacgactaca tcgtgatgcc ggtggtgcag gagattgtga gagaagctca
     2761 ttatagcgtg ggccgacctg atacctacga caaaaacaat atcttctacc tcaccaacag
     2821 ccagtttgcc tatgtggccg gtgtcaatgg gatcatgatc cgtgagaaga tggctaccaa
     2881 cttcttcctg ggcttctttg ccgcggaagc tctgttgatg actgagactg gaaacacggt
     2941 gggtgccgtt cagatagccg gctcggacgc catcacccag atccccttct tcatcaccac
     3001 ctgcgactac actctgatcg gcgaagagct ctatgccgcc tccgcctatc tgaaccgtga
     3061 gcctatgtta ctgggcaccc tcaaggcgca ggactatctg aagatcatca tcgtcttcct
     3121 ggttttggtg ggcaccatcc tgtccacgct ccaggtgacg aatctgctaa atatacttcc
     3181 taccaagtga tagaggacct tatatgaaaa agaccgtacc gttgatcata gtcatcgtcg
     3241 gtggcatcgt cttcctcttg tgggtgttta taccccacaa gatcatggcc gatgacttct
     3301 actaccagtt ctacacaccc tggtcacgtg ccatggcccc gtttgccatg ctgctgggcg
     3361 tgatgagcct ctccatgatg cacaatgcca agatcaagcg ccgcgcgccc aaatggcaat
     3421 tcagctattt cttctttgcc ggattcatcg tcacctccct ggccggattc attggaggca
     3481 cccagaaggg cggcctctat atgtggatgt tcgagaccat ggcgatgccg atgagcgcca
     3541 ccatgttttc cctgctggct ttttatatgg cctccgcggc ctacaaggcc ttcagggcca
     3601 gaagcgtgga agccaccgtc ctgcttgtgg cagcgattgt ggtgatgctg gcccaggtgc
     3661 ctctgggagt gaagatatcc aaacacctgc ctacgatttc gcaatggatc ctggacgtgc
     3721 caaacctggc ttccaagcgt ggaattatgc tgggggtagg cctcggttcc gtggccacca
     3781 ctctcaagat catgctgggt attgaacgct cctatctggg cggcggggat taaaggaagg
     3841 aaaaagatga atatattcga acgcatccaa aagctggacc gccgctggat ctatattgtg
     3901 gtggccctgg ccatcgtgat tccattggcg atccctttca attcagacaa cgttacctct
     3961 ccttccactg aaaacctcta tcaattcatc gattcctatg ccggccgcca ggaccgcgcc
     4021 atcctgttga gcttttatca cgacgcctcc accatgccag agctctttcc catggaagtg
     4081 gccatcctga ggcactgctt cgagcgcaac gtcaaggtgt ttatgctcac ctggtttcct
     4141 cagggcgctc ccatcataga ctatgccatc aacaccgtca aagaggaata ccccaatatc
     4201 aaatccggag tggactactg caactttggc tatattcctg ccgccctggc catgcctacc
     4261 attctgggta tgggagacaa catagccgcc gcagtaaaaa ctgacgccga aggacgcaag
     4321 ctggaaaacc tcgagatcat caaaggggta aacaactaca gcgaaatgaa cctggtggtg
     4381 gagttttccg gctcctcagc cggtgtgacc tggctctatt atgccaggcc caagtttggc
     4441 ctcaatattg gtgtgggtgt cacagccgtg atggctgccg acgaatatcc cttcctgcag
     4501 tccggacaga agatcggcat gctggcaggg ctcaaaggcg ccgcggaata tgaaaagctg
     4561 gtggacgtct ttgccgctta ccgcgaacct ggcagtccca tcaccgttac cgtggatgaa
     4621 gacggcaaca agatcgtgcc cggcagagct ttcagcagcg atatcctcag ggaagaatcc
     4681 accaaacaac tgatcaatat caccactcag acggtggcaa aattcagcgc tgacgaatat
     4741 agcaaattcg tgggcaagta tcccgacaaa gcccccatct ttgacaagct gaaaactgaa
     4801 aagaacggcc aggtggagat cgacgtcacc aaggtaaccg acgatcaaaa ggaagccctg
     4861 ggctataccg ccatggctga tctgaaccgc atgacccgca atatcatcta caaattcaag
     4921 gtggcccgca tcggtatgaa tgcccagtcc gtggcccaca tcatgatcat cgtgtttatc
     4981 gtcctgggca acatcggcta ctttattcag aaagcccgcc aggccaggca gtaaggagga
     5041 aaaagtgaca ttcgaactat tcagcaatct cgtgggtgcc tttttcaccc tctccatatt
     5101 ctccttcctc tataaggaca atcctttcta ccagatgtcc gaacagctgc tggtggggat
     5161 ttctctggga tacggtctgg tgcttaccta tgaacgtgtg ttcatcccct atctgtggca
     5221 gccgatcgtc ctgaaacatc agtttatcct catcattccg gccctcctgg gtgccttcta
     5281 cctgttcagg ttctcacgca agctgagctg gctttcccgc tatcccatcg ctttctcgat
     5341 gatgtatgtg ggcgccagtg tggctctggc catgcatagc tatatcctgg tgcagatgcg
     5401 tcaggctatg gtgccaatcg agaacgtcaa cctgttactg atcttcatcg ggaccgtttc
     5461 ggtgctgctg tatttcttct tttcgaaagc tcacaccggt gcctatggca ggtttgtggc
     5521 agtcggaaaa tggtatatga tggtgggatt cggggccagt ttcgggctta ccgtgatggc
     5581 acgtatctct ctgttgatcg gtcgcatcca gtttctggtc aacgatgtct ttggcaagat
     5641 attctagcct gagctgatct atcctgaagc cgcgggagct ttttccaaaa aggctcccgg
     5701 gggtttgggg acccggaaac gggatcgagg aaataattaa atgaaaaaag cagctatagt
     5761 aaccgccatg gccctgctgg gcatcctgat gctcttctca ggaagcggag cggggtttga
     5821 gtccggtggc ctgatcgaag gccttgtggc catggacgct cccaacgaca gcggccaggg
     5881 cgtcatcctc aaatggaaac cgctcgacaa atctcaccgg atcatccagt acaagatcta
     5941 ccggggcgcc agtcctgaca gcctgttcta tttcaacaat ctggatgtgg accccgtgtt
     6001 gggagtgatc ggcaacgaga tcactttttc agatcaggat taccagcctc tcttcgagtt
     6061 tgagacggcc cccaacaagc tcaaaaagga aaggcaccag ccctccggat ctccgctcta
     6121 ccgtgccgtg ccacgtgatc ccaaagtgat aggatcgctg atcccccatt ttgaggtgct
     6181 gggagccatc aatcatcgcg tgttcaacca ccattcccgc aggatcgact cggccgatgg
     6241 cagcatggca ggttataagc tcaaccagtt tgattttatc tatgccaatc ccctgcccgg
     6301 caagacctat tattacagtg tcgttgccgt caacgaacgc ggcaaacatc tgcccggggc
     6361 caaagtggta tccgccacac cggtggacaa ccgtccgtct ccagatgccg agctggccgc
     6421 cacctgggtg gaagacaaag ctgagatcgg tttcgagtgg agcatgccgg aaatgggcta
     6481 cgacctccag gaatacagcg gctggctgct tcccaaagac caggccccgc tcttcaaggc
     6541 ccagcaggag ctcaactcca gcgctccgga cagccttttc aacgctcaat ggaaagccag
     6601 tgccatcccg atgtttcagg tggccgccga tcctaatgtc tcgaccttgt atcagaaggt
     6661 aaacctgaag gaagcaggca tccagctcag ccgtcccgcc actgaatatc tgcccctgct
     6721 cacctatagc gacacctccg gcttccaaaa cgcttccctg gctgacactc tgtatgtgaa
     6781 gaattcatcc cagtatcctc ccctgccggc tttcagcgtg cacaacaaga aaaacaacaa
     6841 gggagacaac ctgctgatct cagtgggaaa accggttgcc tacatcactc aggcctctta
     6901 tacgtcgggt tccaagacca aactcaagtt caactacgac ctgctggaaa acgagctcta
     6961 tcccatcgag cgcttgcgct tcacatttac cgacacgcat ggcaaagaac tggccacgat
     7021 cacagaatac tatccggaca agatcctcta cctcaaggta ccgcagggct ttaagggcac
     7081 caaaaagttc atggtgagca cccgggtaat gctgcgctcc aaaaaagcgt atgtggagcc
     7141 tgctgccacg caggacatcg tgtttgacga tgccacccgt cgctatgtgg gcaaaaatct
     7201 cagcctgcat ggccatcgcc tggacaaagt gtatctggat gtgcttacca gaaagaacct
     7261 ggaccaggtt ttcaagcccg gcatgcggtc caacggaatg ctgagggccc aggaccacac
     7321 aatcagcttc ccggatgtgc tctataaatc cgtcaccggc tacgacgcca agacccgcag
     7381 cctcaccatc gatcccacca ttagtgttgc catgaatgcc gaaaaaggat ttgccttcca
     7441 agcctctcta tataggagcg actttgagaa agacctcaaa gaaatgtctg ccaaagccga
     7501 cagcctcagg ggagtggtca aacagctggc cgcccagggt gacactctca gccaggcata
     7561 tctggcagcc aaagaggaag gtgactacta tagcggcagc tatggtttta tcaccgctca
     7621 tccagcttac aaacaagccc aaaaggccag gtccgaatcc gcctggcgca gaatcctgct
     7681 ggctgaatca gtccgcaaca accgcagctt cagctatcag tttctactca cggacggcca
     7741 tggtttcttt cagcagaccg atacctacaa ccgcgacggt gaaacggttt tcactcccaa
     7801 accggtatgg ttcaacacca ccatgtgggc caccctgttt ggcagtataa tctttggcat
     7861 cctgatcgta tacgccttgt tccaggcccg ccgcaaagac ctgtatatca ggcccattgc
     7921 cggacttgag gacctcgaca acgccgtggg ccgtgccact gaaatgggcc gtcccgtgat
     7981 gtttgtgccc ggctggggca ctttgggaga accctgcacc atctcctttt tgatgatcct
     8041 cagtcagatt gccaaaaaga cggctgagtt tgatgtaagg ctgatctctc cgcatgtaga
     8101 ctactttgtg gtgcccatcg cccaggagat cgttcagacc tcttacagcg aagtgggcag
     8161 accggatgcc ttcaaccggg aagacatctt cttcatctcg gattcccagt ttgcctttgc
     8221 cgctggcgtc aatggcatca ccatccgtga acgggtggcc accatcatgt atatgggata
     8281 tttcaacgcg gaagctctgc tcatgaccga aaccggtaat cagaccggcg ctattcagat
     8341 tgccggcacc gatgccatca ctcaggtgcc attctttatt accacctgcg attataccct
     8401 gatcggcgaa gagttttatg ccgcgtctgc ctatctctcc aaaaacgtcg aactggtcag
     8461 catgctcaaa ggccaggatt actttaagat ggtgatgatc acgctgatcg taatcggcag
     8521 catcctctcc accttccact ggcacggact tttgcacttc ctgcctttcg agtagcctta
     8581 tggaaaagat cactattccc accagcactc tcaagcggct ccctcagtat ctggaggtgc
     8641 tcaagcagct caaacgcgct ggcctgaaaa gcgtgacagc caccaagata tcggtattcg
     8701 cggatattca tcagaccctg gtgcgcaaag atctctcttt cgcgggtgtg acaaacattc
     8761 cccagagcgg atacaaaatc cgcgatctgg cggcagccat cgaaaagctg ctcaactgga
     8821 atgataccag ttccagctat ctggtgggag tggggcactt gggttccgcg ctgctgggat
     8881 atcaggagtt ctctcaaaaa ggcctcgcca tcatggccgc ctttgataac gatccagacc
     8941 gcatcggctc ggtagtgcat ggcgtgccgg tccactcagt gctggatttc actgccatgg
     9001 ccacccgcaa caacatccgg atcggcatta ttaccgttcc tcccgaagcg gctcagctga
     9061 tcgccgatat catggtccac agcggaatca aggccatctg gaattttact ccccaaaaga
     9121 ttcaggtgcc gcctgatatc atcgtggagt atgtagatat gtttgcttcg ctggcggtgc
     9181 tgagtcgccg gctggcagaa aaaggctgat ttatgcccct caaagtattg ctcgtttccc
     9241 ccaaggccac cacgggtggc ctggagggaa tgagaaaagg ccatcaaatc catcagggcc
     9301 ttctgtttgt agccgccgcg accagggatg ccggtcacag acctgtggtc gtaagcgcgg
     9361 acagcatcag catccatcgt tttatccgtc gttacaagcc agacgtggta ggtttctcct
     9421 gcgtgacggc cacctatccc atgacccggg acctcatcaa gctggtcaaa aaggcgtatc
     9481 cttccatcac caccgtgatc ggaggccatc atgtgacctt tatgtacaaa gagaccatcg
     9541 aggaatccgg agtgaactat gtctgccgcg gcgagggcga agaggtgttt cccgcgctct
     9601 tgaactcgct ggaagtggga gaacgctatc ccgccattcc gggaatggtc ttcaaaaagc
     9661 aaggcaagtt tcacaacgac gagatcatcg ccctggtcga ggatatcaac actctgcccc
     9721 gcatcacgat ggacctcgtg gctccggatt tcagcttcac ccccaagatc gtgagcagcc
     9781 ggggttgtcc cttcttttgt tcctattgct ccatctcagc tttctatggc ggcaagtatc
     9841 gccagcgcag agtggaagac gtgatcaagg atatcgaaga ctacatctcc tggggctaca
     9901 accacttctg gtttcacgac gacaacctta ccgtggatgc caaatgggta aacagcttct
     9961 gcgaactggt ggaggaaaag cagcttaaat tcacctggaa cagtatgagc cgcgtggacc
    10021 tcatctgccg cgatcccgtt ttgatctcca gaatggcccg ctgcggatgc aggatgctgt
    10081 ccatcggcat cgaaagcggc gtgccggaag tgctggaaag catccacaaa aagatcaacc
    10141 tggaccaaat ccacatggcc gttaagatca tgcaaagtgt gggcatcagc tacgactggt
    10201 ttatgctgct gggcagcgga gaccagtttg acacccccgg gataatcgaa aagaacatcc
    10261 agtttttcag caagctgccc ggcatgatcc ttgtctccat tctcacaccc tttcctggca
    10321 cggaagtctt tgagcgcctc agaaaagaag accgcatccg tcattacaac tgggaggatt
    10381 acgacatcac ccactgcgtt tacaaccccc tggggatgag ctacaagcag atggaggcct
    10441 acctccccat cgcctaccgc aggatttacc tggccaaggg actgggactg gtgccgctct
    10501 tcattcacgg agtgcgcacc aaggctatcc ggctggatat ggtcaccagc ggcatcaaat
    10561 ctctctttca gacctttgtg ctgaaaaaag atttctacag ctcgatccga aagacccatt
    10621 agtctggtta actggattaa acagctggta aacatatctg ttagaatcct tgtgccaggt
    10681 ctttatcccc aggccgtaag gcctgataaa aataggaatt gacacaaaat actcatatgt
    10741 gattagttgt taaacgccga atggaaattt gtccacagtc cgtcctgaaa tattcaggaa
    10801 gggcttgcag attataaaaa agtaattaat aatgtcttga gcctgataag agaaccggcg
    10861 cgaagtttac agcaccggat gagacagagt aaccatcacg gctctttgag ccgcgaacaa
    10921 agcaagccat atacatggag gaacgatgaa aaagacattg cttttagtgg cagccgtgct
    10981 gctagtctgc accctgcttc aggccgacat catgccaccc ggccagatca agcctaccct
    11041 cgaaagcccc aggggcatct cgatgcccgg cacccgcgaa gtgcccgctt acacctttac
    11101 ccgcgtcccc aattccatca tagtgaatta ctatgactac atgatcgggg cttacagcag
    11161 cctgccgctg agggtgatcc cttccagcgc tggcggtgga tactttatca cctatcatgg
    11221 acgtcgtcag gctaccagca cccgccgggt gttttacacc tacatgaacc cccagggtga
    11281 tgtgatcagc aacaacgaga tcacaagtgt ccaaaaccat gaaggctttc ccagcatggt
    11341 gatcgatccc gtctccggca aaccgcttta tgcctggcat gccaacgcgg acgccgatgc
    11401 caacctcgaa actcagtttg tctccgatgc ctttatggct ggttttgccg gtctgtggaa
    11461 tgatattcag ctcaccgctg acgctcccac ctcgatcaca gctcccaacg gcaccaccac
    11521 caccgacaac gaattcatct ggcccaccat gacgataggc ccctcaccca tagctgggaa
    11581 acgccgcgtc tatatctctc agcgcaactt catcagtcat gccgccaacg gcagccccag
    11641 cgaaaacgtc tatatcgcct acaccgactt caacggcgac gacatcgaat ctggcgtccc
    11701 tctggtatgg aatcacacta ccctgccaga atatgacgcc tggaacgtgg ataccgcaga
    11761 gtttcgccgt cccacttcct cgatcacggc agacatgtct ggaaacattt actacatcgg
    11821 atatcacgct gccatggacc tcgacaacaa cagcctcgac gagcccgatc tggacgtctt
    11881 tatctgcccg aattacggag aaggtacctg gtcccgcatg acccgttaca gcaagctgcc
    11941 agcctggaat cccaacagcg ctcccagcga caccactggc tattataccg atccggacaa
    12001 tggcgacatc ccttatggcg acaacgagat cttcttccag gtagccaatt cctcccacat
    12061 gaactccgtg gttgaccagc gtggcagagt gcacaccgcc gctctttggg ccctctcttc
    12121 ttacaacgat tattattatc ccgctttgca gtttgtgaag cacatggtct acaatccgga
    12181 ttccaatcag atccaggtca atgacctcta tccccagaaa gatcccagcg ataccttcaa
    12241 tcagaccttt atgccctggg ataccgaagc tccctttggt gaggtggacg agtatgggga
    12301 tgacggtgcc ggtggatact atcctttgat ggtgacggat tggccattcc cgcattggga
    12361 tgcgaccgcc cacaccgatg ccatgatgtt ccactacaac ggagtgagaa tctccgaggc
    12421 caacgatcat ggcatgatgg cagccgtgtg gcaggattcc cagcgcgcca gaatgttcaa
    12481 ctactattct gatacggact attcccagtt tgccaacact cccgagatct acatctccgt
    12541 gagccccaac aatggcgaca cctggagcga gcccatcgtg ctcaacaacg tggaaactcc
    12601 tgaactggcc ggccgcaagc ccatgtatgt ctatcctgcc gacaaggtga tcttcaccgg
    12661 tatgcaaggc aaccaggaag tgggcaagct cggcatcctc ttctacgatg actatacctg
    12721 gggatcaaac gtcaacgccc ctgcctatca tcccactccc gatggcggcg aagtggtgtt
    12781 tatggaactg cagatcgtct tcccctacgg agtggacagc gacgaccccg tggtcacacc
    12841 cgctccctca attctgcatc ccaacttccc caatcccttc aaccccagca ccaccatcag
    12901 ctttgacatg cccaaatccg gccatgccag actgagcgtc tataacgtca aaggccagct
    12961 ggtgcgcgct ctgctcaacg aacagcgcgg cttcggcagc catagcgtgg tttgggacgg
    13021 caccgatgcc aacggcaata ccgtgcccag cggactctat ttctaccgcc tgaatacaga
    13081 caaccattcc gaaacccgca agatgatgct gatgaaata
//
LOCUS       DAOSFN010000067        12393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4286_length_12393_cov_10.012158, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000067 DAOSFN010000000
VERSION     DAOSFN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12393
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4286_length_12393_cov_10.012158"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            611..859
                     /locus_tag="PLO35_05655"
     CDS             611..859
                     /locus_tag="PLO35_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOH79188.1"
                     /translation="MNTDKVKSLAMNDNGFIFDPASGFSYSANETGLYIMKQLGAGLS
                     KEQIIAQLMEDYEVREDDCASDLEHYFLMLESLDLIER"
     gene            856..1959
                     /locus_tag="PLO35_05660"
     CDS             856..1959
                     /locus_tag="PLO35_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOH79189.1"
                     /translation="MNSYPDLSNLDITIAVSGLKTGDNPQPGVPVIRSLRAAGFKGKV
                     VGLVYDSMESGVYVDGLVDAAFLMPYPSAGAEAFLNRLDQILPSQSIDVVIPTLDAEI
                     MQYIRLEDELKRRGIKAFLPTEECFLLRDKTKLANYFPPKGIAVPRTLLVSEPERIPE
                     AKDDLPYPMMVKGRYYEAFKAHNRFEGIKYFHEISERWGLPIIFQKLVPGEEYNIVVV
                     GDGDGGMLGMVAQKKLVITDKGKGFGGVVIKNAQLEDFAQRIISALKWRGPCELEVMM
                     GHDGVCYLIEINPRFPAWVRLAEGAGQNQPALAVLGALGETLKPLPEAKAGTMFIRHA
                     EDLIADIATMGELTVRSELIKHGQADKFAGPQR"
     gene            1966..3315
                     /locus_tag="PLO35_05665"
     CDS             1966..3315
                     /locus_tag="PLO35_05665"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOH79190.1"
                     /translation="MKKQYIAPNIERNSAGNINKYGAKSVVRHQESIDGIAIKDLVSQ
                     FGSPLLVFSESQLRKKYRRMQDIMQMHYPHLQQAWSYKTNYLGAICNIYHQEGSWAEV
                     VSGMEYDMARRLGIPGNQIIFNGPGKQAEDITKAVREGARLHIDHLDELYLIEKIARA
                     EGIEPEVAIRVNMDAGIVPIWSRFGFNYENGEAYRAIQRLLSGKAMKLVGLHTHIGTF
                     ILDAHAYYYAAKALLELAARVETELGVVVSYIDLGGGFASQNTLLGSYAPGEFSSPSF
                     DQYASAIGDAFNESHFVASHNPTLILETGRALVDEAGYLISSVLGKKNLPTGERAVIL
                     DAGVNTAITAWWYKLRVIPTRPFPGAYQNTVFYGPLCMNIDVIRSAVPFPDLQTGEAV
                     LIAPMGAYNNTQWMQFIEYRPAIVLISQTGEAELIRQRETLKDIVGRELIPDHLKSF"
     gene            3324..5528
                     /locus_tag="PLO35_05670"
     CDS             3324..5528
                     /locus_tag="PLO35_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOH79191.1"
                     /translation="MKPKPLAFLKSIPHAYATVMFSDHLGVGLALMLLTLVSPVVGLA
                     GLAGLIIGLLSSRILGFEGWESSSGVLGFNSLLIGLTLGYYYPWALMQHNPLPFIALL
                     TIGAVFTQLLYVGLSSLTMSWFKMPSMSLAFSIAATLLWFYLVRSGLFGGTGFVKPGL
                     WDLSFSLPWFWREYFLSMANIVFVTDLLTGILVALVLLFISRIGFMLSLLGWSVCAFL
                     LRFSDMGDTYGMFFPGFNMILIALTLGSVFLIPGKSSYLLAGLGTAAGFLIAHALSGR
                     YTFAAEMAGRVQTLWVPMFAFPMNIVVILTVFALRLRLKQRSPVMNDYGVLHPEKALD
                     AYLSRYKRFSSAGIPQIMMPVSGEWTITQGHEGAHTHKSQWAFAWDFEITDKQGHKCS
                     ENEQDLRDYYCYGKPVQAAAAGYVAKVVHSIPDNPIGVSNTHDNWGNYISIFHSYGFY
                     TLYAHLKEGSVKLKEGDWVKQGEKIGQVGSSGRSPIPHLHFHAQSGPEAGSPTVFSHI
                     VNYRKRDADGSFELVSSGVPAERERVSTLVPEQDLAAILRLGLGQEQSFEVKSGQDIW
                     TETWKVAVDLLGVHRIESDRGTVLEFSIYNGIFNALNLKGRRDSALAAFALAASRLPW
                     AENQLLSWEDEPALSLLLDPVAKNLALFLFPFFKPVRLSYHATVDSAQGTLKLESRAS
                     LKGLGMRLREAQTRVNLSRQDGILDIILTRDQDPVLEAKQILTSDINQEVTQ"
     gene            5525..6124
                     /locus_tag="PLO35_05675"
     CDS             5525..6124
                     /locus_tag="PLO35_05675"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH79192.1"
                     /translation="MKTNLFIVLVLMLAAGALRALPNIEDSYNQENKGYYASALDVMM
                     ELQAADAKEPFYQVRIAWLQYLLGRYEEAIASYRKALALLENLDARTGIINCLLALGR
                     YPEAIAEVDEQLKTHRQNPTLISKGAYAAYMKKDYARAVTYYQTMIAIYPWDFESRGY
                     LVNNLFLAGQNEEGQRQLLLLKKYYPQSAIVQQYKDKLK"
     gene            6128..7384
                     /locus_tag="PLO35_05680"
     CDS             6128..7384
                     /locus_tag="PLO35_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79193.1"
                     /translation="MRKGLLLLIMILMGCRLAAQGLVSDPLSEARKAVAYDPVNDGKR
                     LDLAWNLMLAGQYEEALVHYRLVSSRDSSNTDAATGVLWSLSAQNQHQELIKEADKLI
                     SVMPEAGPLYYYRGLARLHQGKANKARTDEKQALRSAPDSFWQDLAARALSDSYLALD
                     DRPAAKSTLNKHAPQAILPPARKALTIELTAGAKGDSTYIIAAGLKASLGTTRLGFKA
                     EELRLGAEHFRWMFQASALQQFHHFDLFAELNWLEGKDAKVYPAKGTRLGLTPKIYAG
                     PLIFRPRLSQSVLITPRLNAYQSDAGLRLSMPALNLSYSLSWIYLDKEIVDADTSDLA
                     HSADASFSFGRGWELGIYGGSGALAWHNTPYGGVIDDFTPDLSYAGASIYAPIGKRLG
                     LLLYGQLSFDDGDSTPFYYLRGSYRV"
     gene            7377..10088
                     /locus_tag="PLO35_05685"
     CDS             7377..10088
                     /locus_tag="PLO35_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79194.1"
                     /translation="MCKLLPFLLLSLFLSACNLRENALLPPNLDPKDYVESSTILVYS
                     DHLIKSENDASYLYIPKQSIADAALNYGDRISFHRAEALLERDSLAFETDARAFTSTY
                     QIDIIRSGASILLDSIPGFATLYTDMESPASLNDAALVQSAWVLDNSSVNVYPYGSGR
                     CFFDLDGNGDLALMDLGHSDTLELEASGKATQALISRPGGDIHIWFPGDYLSSQLTLS
                     VSDSLSSADLSLTQGLFEGFGLGTPVLSVQTNYQGAAVPILKIESGGKGVFATQWTRI
                     TDGRVQGWPSGDDTWLYEGNTLISFLQGNGRYFLLTPMATQDQITLPLDGTFDQLYLQ
                     DLWLDLNDLNLPNHSLSIDLQPQTQDLIAAYFTGAPFQIGNAWQAFAVTLKEGSATIA
                     SLPDDLWLELGLRAKHSNWAESRLTRVFRTASEDLISYKSYGDAYDEQHFSQSNGFVY
                     SGIAASGTYLLAPISESTSSLNVPCLKPQVYIQTQRLELSWEDSSLPCSSLSFQFDAT
                     QPLGHPWLSGYPYTLSNRKSIMKIEALGRKRAVDTLPEGLFLRHRMNVAPQEVVNFNH
                     SSTFPQWYRYRSVSAFEHNGFVMDGNILQISPAVPGYIFNAANLSHSGTSYGLMSYAR
                     MSWDERGYEVLLDSSTAMPSNSILEITPRTGFTDRLNILASQYQLSLLAPVYAFHMSG
                     NSTFYNDFQPLIRLRQSSRRANHLFSITDGAYYRIFTYDQATQADGWHFGLSDGHVSF
                     YLAYDGEFGAASDQDPHSSVEVTALSNANPLIASLYQAQADIPAQLIGAGMPLGARMI
                     LSEAINPPANAIGSQRVDFRTAQGAILSPNFYNVPLEVPVPYIYVPIDNYSPGMELRM
                     FYRDLSGTVTEFTRVQSFSADPVDEFIVVGNCAVCFINNSGLFYLTP"
     gene            10171..11934
                     /locus_tag="PLO35_05690"
     CDS             10171..11934
                     /locus_tag="PLO35_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOH79195.1"
                     /translation="MQTYLFAILGVAAIWWLWGKVRPDLVALIALFALVYFNVIPAHE
                     AFAGFSHPAVITLAAMMVMSKALQVSGVLDYLARVLNYLGPDPLGQILVLCALTAFAA
                     AFMSNLGAMVIMIPIAIHISGKHGIPVSKVLMPLSFASILGGMSTLVGTAPNLIISSF
                     KESSGSTAFSLFSFIPVGGAVALTGVIFLGLIGWRLLPERHKRRSADDDLSIEDYITE
                     VKVLRESRLAGSTLRKIMQNAAYEVTVIGLAREKLLLEVIDPDEELLPDDILIIEADS
                     ENLKTFMDFTQAKLIGGRKFHKDAEGARNISMREVVILQDSRLVGKSAADINLRSRYG
                     INLLAISRGGRKTIRRVSKSRFRVGDVLLIQGRTQILGEVVSAMGCMPLHERGMQQAD
                     PYRAVLSLGIFGVAIAMMLLGLLPAHMSLAMAALLLALTNVIAPREIYHHIDWSTIVL
                     LATLIPVANALLNGGGAQSIAGLILHLATGTPVWVSVGLIIALCMLLANLISNVATAV
                     LMAPVAILTAQLSGASSEMFLMAVAIGASSTFVTPFRHQSNTIVMGPGSYRFGDFVKL
                     GLPMQVLVLLSATPLLYWYWH"
BASE COUNT         2688 a         3575 c         3394 g         2736 t
ORIGIN      
        1 gtctgggcgc tttggacttc aagcaggata tcggagcaat aatgttcgag agtgtactgg
       61 ttgaagtcca aacggcccgc aaactcgttg tcatgcatac atttatcgtg gccgcttgaa
      121 ctccaacggg tcgtgcacgt acgcaccgac taaagagctt gtgaaaacag cgtattgagg
      181 gagggatttt cagtgagaat catgcgtccc atgatcccga tgattcccac cagggactca
      241 gaccgttgac cgggtagtat gccgctggtt attcaggttc cgatcacctg agcatcactc
      301 gtgcctaacc catgccaaag cacgcgatag gcgtgcgata agcacgggat aagcgtgcga
      361 tagtcaagtg ggagcaatgg tccaggatcc ggaccggaag agggaacatc cagccagcag
      421 acaggttcga tcccatcacg ttgttaaata ctgattgaca gatatatggc tttagaattg
      481 caatgccaaa cttccctgtt tataatgaca actatatttt gagtgcggta atgttgtagt
      541 ccgcaagtat tattagagac gtgccaccct gtgtgtgggg aggcagcact aaaagtatcc
      601 gggaggatta atgaacaccg acaaagttaa gagccttgcc atgaatgaca acggctttat
      661 tttcgacccg gccagcggct tcagctacag tgccaacgag acagggctgt acatcatgaa
      721 gcagctcggg gccgggctat ccaaagagca gatcatagct cagctgatgg aagattatga
      781 ggtgcgggaa gatgactgcg ccagcgatct ggaacactac tttttgatgc tggaatctct
      841 ggacctgatc gagcgatgaa cagctatcct gacctgagta atctggacat cacgattgcc
      901 gtctccggtc ttaaaacggg agacaatccc caacccggag ttccggtaat ccgcagttta
      961 agggccgccg gtttcaaagg caaagtggtg ggcctggtgt atgattcgat ggaatccggc
     1021 gtctatgtgg acggtctcgt ggatgcggct tttctgatgc cctatccctc cgcgggcgct
     1081 gaggctttcc tgaatcgcct ggaccagatt ttgccctccc agagcatcga tgtggtgatt
     1141 cccaccctcg atgccgaaat catgcagtat atccgcctcg aagacgaact gaagcggcgc
     1201 ggcatcaaag cctttctacc cacggaggag tgtttcctcc tgagagacaa gaccaagctg
     1261 gccaactatt tccctcccaa agggatagct gtcccgcgca cgctgctggt ctccgaaccg
     1321 gaacgcatcc ccgaagccaa agacgacctg ccctatccga tgatggtcaa aggccgttac
     1381 tatgaggctt tcaaggccca caaccgcttt gaggggatca agtatttcca tgagatcagc
     1441 gagcgctggg gcctgcccat catctttcaa aagctggtgc ccggcgaaga gtataacatt
     1501 gtggtagtgg gagatggaga tggcggcatg ctgggcatgg tggcccagaa aaagctggtg
     1561 atcacagaca aaggcaaagg ctttggcggc gtggtgatca aaaacgccca gctggaagat
     1621 tttgcccagc gaatcatctc tgccctcaaa tggcgcgggc cttgtgaact ggaagtgatg
     1681 atgggtcacg atggcgtctg ttatctgatc gagatcaatc cccgtttccc tgcctgggtt
     1741 cgtctggccg aaggggccgg acagaatcag cccgctctgg cggtgctggg agcgctggga
     1801 gaaactttga aacctttacc ggaggccaag gccggaacga tgtttatccg gcacgccgaa
     1861 gacctgatag cggacatcgc caccatgggc gaactcacgg tccgcagcga attgatcaag
     1921 cacgggcaag cggacaagtt tgccggtcca cagaggtaag tgactatgaa aaagcaatat
     1981 atcgccccca atatcgaacg caactccgcg ggaaacatca ataaatacgg cgccaagagt
     2041 gtggtccgtc atcaggaaag catcgacggc atcgcgatca aagacctggt gagtcagttt
     2101 ggctcgccac tgctggtttt ttccgaatcc cagcttcgca agaagtatcg ccgcatgcag
     2161 gatatcatgc agatgcacta tcctcatctg cagcaggcct ggagctataa gaccaactat
     2221 ctgggcgcca tctgcaacat ctatcatcag gaaggttcct gggccgaagt ggtttccgga
     2281 atggaatatg acatggcgcg acgcctcggc atccctggaa atcagatcat tttcaacggc
     2341 ccgggcaagc aggctgagga tatcaccaaa gccgtgaggg aaggtgccag gctgcacatc
     2401 gaccacctgg acgagctcta tctgatcgaa aagatcgccc gcgccgaggg catcgagcct
     2461 gaagtggcga tccgcgtcaa tatggatgcc ggcattgtac ctatctggag ccgttttggc
     2521 ttcaattatg aaaacggaga agcctaccgg gccatccagc gtctgctttc cggcaaggcc
     2581 atgaagctgg tgggcctgca cacccacatc ggcaccttta tcctggacgc ccatgcctat
     2641 tattatgccg ccaaagccct gttggagctg gccgccaggg tcgaaaccga actgggggtg
     2701 gtggtgagct acatcgatct ggggggtggt ttcgcttccc aaaacactct gctgggctct
     2761 tacgcgccgg gtgaattcag ttccccctcc ttcgatcaat atgccagcgc cattggcgac
     2821 gccttcaatg aatcccactt tgtcgcctcc cacaatccta ccctgatatt ggaaacgggt
     2881 cgcgcgctcg tggatgaagc gggctatctg atcagcagcg tgctcggcaa gaaaaacctg
     2941 cccaccggtg agcgggctgt gatcctggac gccggggtca atacagccat cacagcctgg
     3001 tggtacaagc tgcgcgtcat ccccacccgt ccttttccag gggcctatca aaacaccgtg
     3061 ttttacggtc ccctctgcat gaatatcgac gtcatccgtt ccgctgtccc cttccccgat
     3121 ctgcagaccg gagaggcggt attgatagct cccatgggcg cttacaacaa tacgcagtgg
     3181 atgcagttca tagaataccg gccagccatc gtgctgattt cccagaccgg agaggcggaa
     3241 ttgatccgcc agcgggaaac cctgaaggat atcgtggggc gggaattgat cccagaccat
     3301 ctgaaaagct tttaggatag ctggtgaagc ccaaaccgct ggcctttctg aagtccatcc
     3361 cgcatgccta cgccacggtg atgttttctg atcacctggg tgtggggctg gccctgatgc
     3421 tgctcacgct ggtatctcca gtggtgggcc tggcgggttt ggcgggattg atcattggcc
     3481 tcttgagctc cagaatcctg ggctttgaag gctgggaatc atccagcggg gttttggggt
     3541 ttaacagcct cctgatcggc ctcactctgg gctactatta tccctgggct ctgatgcagc
     3601 ataatcccct gcccttcata gccttgctga cgattggggc cgtctttacc cagctgctct
     3661 atgtgggcct cagttcgctc accatgagct ggtttaagat gccgtccatg agcctggctt
     3721 tttccatcgc cgctacgctg ttgtggtttt atctggtgag aagcggtctc tttggcggga
     3781 cgggatttgt gaagccgggg ctttgggacc tcagtttcag ccttccctgg ttttggcggg
     3841 agtactttct cagcatggcc aatatcgtgt ttgtgactga tctgctcacc ggcatcctgg
     3901 tggcgctggt gctgctcttc atctccagga tcggctttat gctttcgctg ctgggctgga
     3961 gcgtatgcgc tttcctgctc agattcagcg acatgggtga cacctacggg atgtttttcc
     4021 cgggtttcaa catgatcctg atcgcgctca cgctgggctc ggtgtttctg atccccggca
     4081 agagcagcta cctgctggca ggcttgggaa cggcggcggg ctttctgatc gctcacgctc
     4141 tttcgggacg ctacaccttc gcggctgaaa tggccggaag agtgcaaacc ctctgggtgc
     4201 cgatgtttgc cttcccgatg aatatcgtgg tcatcctcac cgtctttgcc ctccggctga
     4261 ggctgaaaca gcgttcgccg gtgatgaacg actatggagt gttgcatccc gagaaagcac
     4321 tcgatgccta cctgagccgc tacaaacgct tttccagtgc gggaatccct cagattatga
     4381 tgccggtgag cggagaatgg accatcacgc aaggccatga gggagctcac actcacaaaa
     4441 gccagtgggc ctttgcctgg gattttgaga tcacagacaa gcagggccat aaatgctccg
     4501 aaaacgagca ggacctgcgg gattactatt gctatggaaa gccggtccag gctgccgcgg
     4561 caggttatgt ggccaaagtg gtgcacagca ttccggacaa ccccatcggg gtctcaaaca
     4621 ctcacgacaa ctggggaaac tacatctcca tcttccacag ctatggcttt tacaccctct
     4681 atgcccatct caaagagggc tctgtcaagc tcaaggaagg cgactgggtg aaacaaggtg
     4741 aaaagattgg ccaggtgggc tcttcaggcc gctctcccat cccccatctg cactttcacg
     4801 ctcagagcgg tcccgaggcc gggtcaccca ccgtgttcag tcacatcgtc aactatcgca
     4861 aacgcgacgc ggacggcagc ttcgagctgg tcagcagtgg cgttccagca gaacgggaac
     4921 gcgtctccac cctcgttccg gagcaggacc tggccgccat cctgagattg ggactggggc
     4981 aggaacagag ctttgaagtc aaatccggcc aggacatctg gaccgagacc tggaaggttg
     5041 cggtggacct gttgggagtg caccgcatcg aaagcgaccg cgggaccgtg ctggaattct
     5101 ccatatacaa cggcattttc aacgccctca acctgaaagg caggcgcgac agcgctttgg
     5161 cagcgttcgc actggccgct tccagattgc cctgggcgga aaatcagttg ctgagttggg
     5221 aggacgaacc agccctgtct ttgctgcttg acccggtggc caaaaacctg gccctctttc
     5281 tcttcccctt cttcaagccg gtgcgcctgt cctaccacgc gaccgtggat tcagcccagg
     5341 gaactctgaa gctggaatcc cgggcgagtt tgaaaggctt gggcatgaga ctgcgggaag
     5401 ctcaaaccag ggtaaatctt agccgtcagg atgggatcct ggacatcatc ctcacccgag
     5461 atcaggaccc cgtcctggaa gcaaaacaaa tactcaccag cgatatcaat caagaggtaa
     5521 cgcaatgaag acaaatctgt ttatcgtgct ggtcctgatg ctcgccgctg gagctctgag
     5581 ggccctgccc aacatcgagg atagctacaa tcaggaaaac aaaggctatt acgcctccgc
     5641 tctggatgtg atgatggaac tgcaggccgc cgatgccaaa gagccctttt accaggtcag
     5701 gatcgcctgg ctgcagtatc tgctgggccg ctatgaagaa gctatcgcct cctatcgcaa
     5761 ggctttggca ttgctggaaa acctcgacgc ccgcaccggc atcatcaact gcctgctcgc
     5821 tctgggcaga tatcctgaag cgatcgcgga agtcgatgag cagctcaaga cccatcgtca
     5881 gaaccccacc ctcatctcca aaggggctta tgccgcttac atgaaaaagg actacgccag
     5941 ggccgtgacc tattatcaga ccatgatcgc gatctatcca tgggactttg aaagccgtgg
     6001 ctatctggtg aacaacctgt ttctggccgg gcagaacgag gaaggccagc gacagctgct
     6061 cctgctcaaa aagtattatc cccagtctgc cattgtgcag cagtacaagg acaaactcaa
     6121 gtagtctatg cgcaaaggct tactgcttct gatcatgatc ctgatgggct gcaggcttgc
     6181 ggcccagggc ctggtttctg accccctcag cgaagccaga aaagcggtgg cctatgaccc
     6241 tgtcaacgat ggcaagcggc tcgacctggc ctggaacctg atgctggccg gccagtatga
     6301 ggaagcgcta gttcattatc gcctggtgag ttccagagac agcagcaata ccgatgccgc
     6361 gaccggggtg ctgtggtctt tgagcgctca aaaccagcat caggagctca tcaaagaagc
     6421 tgacaagctt ataagtgtga tgcctgaggc gggaccgctg tactattatc gcggcctggc
     6481 cagattgcat caggggaagg caaacaaggc cagaacggac gagaagcagg ccctcaggtc
     6541 cgcgccggac agcttctggc aagacctcgc cgccagggca ctcagtgaca gctatctggc
     6601 cctggacgac cgcccggcgg ccaaatccac cttgaataaa cacgctcccc aggccatcct
     6661 gccaccagcc cgaaaggctc tgacgatcga gctcaccgca ggcgcgaaag gggattccac
     6721 ctacatcata gctgctggcc taaaagcctc tctgggcacc accagactgg gcttcaaagc
     6781 ggaagagctc aggctgggcg ctgaacattt cagatggatg ttccaggcct cagcgctcca
     6841 gcaattccac cactttgacc tctttgcgga gctcaactgg ctggagggaa aggacgccaa
     6901 ggtttatccc gccaagggaa ccaggcttgg tctgacgccc aaaatctatg ccggtcccct
     6961 gatcttcagg ccccggctga gccagtctgt cctgatcacc ccgcgcctca atgcctatca
     7021 aagcgacgcg ggcctaaggc tgagcatgcc agccttgaac ttatcctaca gtctgtcatg
     7081 gatatacctc gataaagaga tcgtggatgc cgatacctca gacctggccc actctgccga
     7141 tgccagcttc agttttggca gaggctggga actgggcatc tatggcggca gcggtgccct
     7201 ggcctggcac aacactccct acggcggagt gatcgacgac tttactcccg acctatccta
     7261 cgcgggcgca tctatttatg cccccattgg caagcgcctg gggctgctgc tctatggcca
     7321 gctgagcttt gatgatggcg attccactcc attctattac ctcagaggtt cctaccgtgt
     7381 gtaaactttt gcctttcctg ctgctgagcc tgttcttaag tgcctgcaac ctcagagaaa
     7441 acgccctcct gcctcccaac ctcgatccca aggactatgt ggagtccagc accatcctgg
     7501 tctattccga tcatctgatc aagagcgaga acgacgccag ctacctctac atccccaaac
     7561 agagcatcgc ggacgcggcc ctgaactatg gagaccggat cagttttcac cgcgcggaag
     7621 ctctgctgga gcgggacagc ctcgctttcg agaccgatgc cagggccttc accagcacat
     7681 atcagattga tattatccgc tccggagcga gcattctgct ggattccatc cccggtttcg
     7741 cgacactcta tacggacatg gaaagtcctg ccagcctgaa cgatgccgct ctggtgcaga
     7801 gcgcctgggt gctggacaac agctccgtga acgtctatcc ttatggttcc gggcgctgtt
     7861 tcttcgatct cgacggcaat ggagacctgg ctttgatgga cctgggccac agcgacaccc
     7921 tggagctgga agccagcgga aaagctactc aagccctgat ttcaaggccc ggaggcgata
     7981 tccatatttg gtttccgggg gactatctga gctcgcaact tactctctct gtatcggata
     8041 gcctcagcag cgccgaccta agcctcaccc aaggcctctt tgagggcttt ggtctgggca
     8101 cgcctgtgct gagcgtgcag acaaactatc agggagcagc ggtgccgatc ctgaagatcg
     8161 agagcggcgg caaaggcgtt tttgccaccc agtggaccag gatcaccgac ggcagagtgc
     8221 agggatggcc ttccggagac gatacctggc tctatgaggg caacactctg atcagcttcc
     8281 tgcagggcaa cggacgctat ttcctcctga cccctatggc tacccaggac cagatcaccc
     8341 tgcccctgga cggaaccttt gaccagctct atctgcaaga cctctggctg gacctcaacg
     8401 atctcaattt gcccaatcac agcctcagca tagatttaca gcctcagacg caagacctga
     8461 tcgccgccta ttttaccggt gctcccttcc agataggaaa cgcctggcag gcctttgccg
     8521 tcaccctgaa agagggctcc gccaccatcg caagcctgcc ggacgacctg tggctggagc
     8581 tgggactgag ggccaaacac tccaactggg cagagagccg tctcacccgt gtcttcagga
     8641 cggcgtctga ggacctgatc agttacaaga gctatggcga cgcctatgac gagcagcact
     8701 tcagccagtc caacggattc gtgtacagcg ggatagctgc cagcggcacc tatctgctgg
     8761 cccccatctc ggaatccaca agcagcctca acgttccgtg cctgaaaccg caggtctata
     8821 tccaaaccca gcgtctggaa ctcagctggg aggacagctc tctgccctgc agctcgctca
     8881 gcttccaatt cgatgccacc cagccgcttg gccatccctg gttgagcgga tatccctaca
     8941 cgctttcaaa ccgcaagtcc atcatgaaga tagaggccct gggccgcaaa cgcgcggtcg
     9001 ataccctgcc ggaaggtctc ttccttcgcc accgcatgaa cgtggccccg caggaagtgg
     9061 tcaacttcaa ccattccagc acgttccctc aatggtaccg ctaccggtcc gtctcagctt
     9121 tcgaacacaa cggctttgtg atggacggca atatcctgca aatctcacca gccgtgccag
     9181 gttatatctt caatgccgcc aatctttcgc acagcggaac aagctatggg ctgatgagtt
     9241 acgctcgcat gagctgggac gaacgcggct atgaagtgct gctcgacagc tccactgcca
     9301 tgccctccaa cagcattctg gaaatcactc ccaggactgg ctttacagac cgcctcaaca
     9361 tcctggccag ccaatatcag ctcagtctgc tggccccggt ctatgctttc cacatgagcg
     9421 gcaacagcac attctacaat gatttccagc cgctgatcag gttgcgtcag agctcccggc
     9481 gcgcgaatca tctcttcagt atcacagacg gagcttatta ccggatattc acctatgatc
     9541 aagccacgca agcggatggc tggcattttg gcctcagtga tgggcacgtg tctttctatc
     9601 tggcctacga tggtgagttt ggcgcggcct cagaccaaga ccctcacagc agtgtggagg
     9661 tgaccgcgct cagcaacgcc aatccgctga tcgcctcact ttatcaggcc caggcggata
     9721 ttccggccca gctgatcgga gcgggtatgc ctctgggggc caggatgatc ctgagcgagg
     9781 ctataaaccc tccggccaat gccataggct ctcaaagagt ggatttcagg acggctcagg
     9841 gcgctatcct cagccccaat ttctacaacg tcccgctgga agtgccggtg ccctatatct
     9901 atgtgccgat agacaattac agccccggaa tggagctcag gatgttctac cgcgatctca
     9961 gcggcaccgt gacggagttt accagggtgc agagcttttc ggccgatccc gtggatgagt
    10021 ttatcgtggt gggaaactgc gcggtttgct ttatcaacaa ctccggtctt ttctatctga
    10081 cgccgtgatc cggaataata gttgcccagg gctgatttgt cgatatcctt tctctatgga
    10141 aagctataag caccggggag tcggatgatc ttgcagacat atttattcgc cattttaggg
    10201 gtggctgcca tctggtggct gtgggggaag gtgcgtccag atttggtggc actcatcgcg
    10261 ttgttcgcgc tggtctattt taacgtgatt cccgcccatg aagccttcgc gggtttttcc
    10321 catcccgccg tgatcactct ggccgccatg atggtgatga gcaaagcctt gcaggtttcc
    10381 ggagtgctgg actatctggc cagggtcttg aactatctgg gccccgatcc cctcggccaa
    10441 atcctcgtgc tctgtgctct cactgcgttc gcggccgctt ttatgagcaa tctcggtgcc
    10501 atggtgatca tgatccccat cgccatccac atcagcggca aacacggcat cccggtttcc
    10561 aaagtgctga tgccgctgtc atttgcctct attttaggag ggatgagcac tctggtgggc
    10621 acagccccca acctcatcat ctccagtttc aaagagagct ccggatcaac tgctttcagc
    10681 ctcttttctt ttattcccgt gggtggagcg gtggccctga cgggggtgat cttcctgggc
    10741 ctgatcggct ggcgtctctt gccggaacgc cataaacgcc gctccgctga tgatgacctt
    10801 tccatcgagg attacatcac cgaggtcaag gtgctgagag aatcgcgtct ggcaggctcc
    10861 accctcagaa agatcatgca aaacgccgcc tatgaggtca ccgtcatcgg tctggccaga
    10921 gaaaaactgc tgctggaagt gatcgatccc gacgaagaac tgctcccgga cgacatcctg
    10981 atcatcgaag cggattctga aaacctcaag acctttatgg acttcacgca ggccaaactg
    11041 atcggcggcc gcaagtttca caaggatgcc gagggcgcca gaaacatctc catgcgcgag
    11101 gtggtgatcc ttcaggattc acggctggtg ggcaaatccg cggccgacat caacctgcgt
    11161 tcccgctatg ggataaacct gctggccatc tcgagaggag gacgcaagac catccgccgg
    11221 gtgagcaaaa gccgcttccg ggtgggagac gtgctgctca tccagggccg cactcagatt
    11281 ctgggggaag tggtctctgc catgggctgt atgcctctgc atgagagagg tatgcaacag
    11341 gcggacccct accgggccgt cctcagtttg ggtatctttg gcgtcgcgat agccatgatg
    11401 ctgctgggac tcctgcccgc ccatatgtct ctggccatgg cagcgctgct gctggccctc
    11461 accaatgtga tcgcccccag ggaaatctat caccacatcg actggtccac gatcgtcctg
    11521 ctggctactc tcatcccggt ggccaatgct ctgctcaatg gaggaggggc ccaaagcatt
    11581 gctggattga tacttcacct ggctacggga acccccgtgt gggtgagcgt ggggctcatc
    11641 atagcgctct gcatgctgct ggccaacctg atcagcaacg tggccaccgc ggtgttaatg
    11701 gcccccgtgg ccatcctgac cgcccagctc agcggcgcgt cctctgagat gtttctgatg
    11761 gctgtggcca tcggggccag ttccaccttc gttaccccct tccggcatca atccaacacc
    11821 attgtcatgg gccccggctc ctatcgcttt ggagattttg tcaagctggg cctgccgatg
    11881 caagtcctgg tgcttctgag cgccacgccc ttgctgtatt ggtattggca ttgaggcacg
    11941 tgctggattg tttcccgcag atttcgcgga tttagcgcgg atcacgcaga tttgaaaagg
    12001 ttgagtttgg ggttgagagg cttgaggcac gcgccggatt ggaatcaaaa tgccaagagt
    12061 tgcggaatct acgggaatct gtagtggtgc tcgcctttac atgtgcagat agcgtctctc
    12121 acccagccgg tcgatcagtg ccaaagtgat atccggcaaa gactttagcc ctctccagga
    12181 cctaatcgta cagctgggtc tggctcattg ttaacagccg tgtaatctca tgtgttgcca
    12241 tgattcctcc atttttgatt ggcctcgcga cgggatgcct ctacaatact atatgccggt
    12301 tggaggctac ggccctgaca aatatctccc cgcttcctat tactggatca ttgcgggatc
    12361 attgtgggtt tgacgcgtaa tgatcacgta atg
//
LOCUS       DAOSFN010000068        12370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4296_length_12370_cov_8.998132, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000068 DAOSFN010000000
VERSION     DAOSFN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12370
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_12370_cov_8.998132"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            753..1097
                     /locus_tag="PLO35_05695"
     CDS             753..1097
                     /locus_tag="PLO35_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH79196.1"
                     /translation="MAVNYIKNLEAGKALKLEALVQYSQGQIVSRTLAQNKAVSLTLF
                     AFWQGEEISSHDSKGDALISVLDGEGEITIGDQTHMVKKGESILMPAEIPHAVKAVTN
                     FKMYLTVVFPDQ"
     gene            complement(1308..2228)
                     /locus_tag="PLO35_05700"
     CDS             complement(1308..2228)
                     /locus_tag="PLO35_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOH79197.1"
                     /translation="MPPKEQISIRLQNRLKRPLIKTLLYVLKVLITAYILYIVFKDID
                     LRELLKSMLRLPLWMFIVLCGGSILRHWAQLRNWRHALSLNPSYKMNDRDVLKSYLIG
                     LPLRFAVPGGPASLGKVFWVDNSSHMASVLAFGIERGFLTWATWSFASGAALFYFTAI
                     PLWLRVIVFTLCLVAPVLGIPILKAWPKGRQALPAYKQLAPRMALLQVGAALIAYSQY
                     WLILNTHIQISWPESLKLMSLTNFSNSIPITLAGLGLRESFAVHFLGEAGFSAAQAIS
                     TTLTLFVVQDIIPALIGLLVFLTRNKPVRV"
     gene            complement(2238..>3314)
                     /locus_tag="PLO35_05705"
     CDS             complement(2238..>3314)
                     /locus_tag="PLO35_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOH79198.1"
                     /translation="YLGGCDAIALCVNNRDANGKPDPARLIEFVKMLEPSVGAVNLED
                     ISQPNCYRVLDELREACQIPVWHDDAQGTACVTLAGVINALELTGKTMGNIKAVLFGA
                     GASNTTIASFLLQSGLDPARLTVFDSKGGIHLGRKDLEMDPTRYKQWALAQNTNPLRN
                     NTMEEAMRDADLLIALSTPGPNTIKPDWIRLMAPKAIVFTCANPVPEIYPYDAKAAGA
                     YIVATGRGDFANQVNNSCGFPGILKGALTVRATKITDSMAIAAAHSLALFARNRGIDP
                     ESIVPTMDETEVFAHEAADVAMQAIADGVAQIQLSREEVFKIAMEDITASRSLTHKMM
                     EDGYLSPPPQDLINQALAACGIED"
     assembly_gap    3315..3414
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3560..3811)
                     /locus_tag="PLO35_05710"
     CDS             complement(3560..3811)
                     /locus_tag="PLO35_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOH79199.1"
                     /translation="MKSEGKTRPSKGTLSSDPINDPINDPINQFLTLLAANPGITYDQ
                     LAQHTGMSTSTVKRHLQRLKAMGLVTRKGSNKNGTWEIK"
     gene            complement(3848..4069)
                     /locus_tag="PLO35_05715"
                     /pseudo
     CDS             complement(3848..4069)
                     /locus_tag="PLO35_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            4302..5624
                     /gene="trmFO"
                     /locus_tag="PLO35_05720"
     CDS             4302..5624
                     /gene="trmFO"
                     /locus_tag="PLO35_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOH79200.1"
                     /translation="MKKTISIIGAGLAGSEAALQLAKRGWEVALYEMRPGVMTPAHQT
                     GLAAELVCSNSLKSTRLDTGAGMLKAEMALLGCQLLPLAEKCKVPAGHALAVDRQLFS
                     EAVEDALKAQDSITRVNEEIKDLPDGPVIISAGPLATDNLMRSLSALIGDQHLYFFDA
                     IAPIVDAASLDSGIVYMKGRYDKGEADYINCPFTKEQYLDFVEALLSGEQHQAHEFEN
                     EFFHDPKFSYYENCTPIEELARRGVDTLRHGVMRPMGLEDPRGGGKPYAVLQLRAENT
                     DLTAYNLVGCQTMLRYGEQKRVFRLIPGLDQAEFLRYGSIHRNAYLDGPKVFNADLSL
                     QARPDVWVAGQLSGVEGYVECIASALIVSRIISEGLALLPETSILGQLWRRLITPQDR
                     KFQPVNANFGLLPDLDSKIRDKKLKKELMAQRGLEAMQAYIDQGEKDE"
     gene            5617..5949
                     /locus_tag="PLO35_05725"
     CDS             5617..5949
                     /locus_tag="PLO35_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOH79201.1"
                     /translation="MNKYLSACGLVCPDCECYKATLSGDLAQKQDIAERWSKNYQASL
                     TVDDIHCEGCMSSGRKFSWCDKCPIRACVVEKGYQSCAECDSFPCQTNAFLYEHVPAA
                     KENILSLR"
     gene            complement(6002..6871)
                     /locus_tag="PLO35_05730"
     CDS             complement(6002..6871)
                     /locus_tag="PLO35_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79202.1"
                     /translation="MTSYLDYLARWFRVLMPAILLLGLGLSHASALKFADQLYSQGDY
                     TAAATEYKRILYQEADNSADSLYAVQMLMRSLFQASDRAGMFQVSHQYAGYLTPADLA
                     ARYNALLMIRDGYYLPAYSLLDRSADPRAKLLRGLSLEYLGKSEEAVTQLRELSLSDS
                     ATARLAEEALRIRQKTTKPHTPSPLLAGAFGILPGLGYAVTGRFETAIASLVINSALL
                     ISSIELYQNDLQWSAGIVFSVFTGFYLGNIYGSASAAAKDRAIYKAAILDEFLKINVD
                     ELLSAPGTIADSP"
     gene            complement(6855..7130)
                     /gene="yidD"
                     /locus_tag="PLO35_05735"
     CDS             complement(6855..7130)
                     /gene="yidD"
                     /locus_tag="PLO35_05735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOH79203.1"
                     /translation="MRVVPSSDYASLIGFYRDYISPVDGERCTMYPGCSSYSAQAVDQ
                     YGAIKGGLMTFDRLIRCGRDGYLYPSIWIEGAELHLDEVKRNNDQLP"
     gene            complement(7379..7885)
                     /locus_tag="PLO35_05740"
     CDS             complement(7379..7885)
                     /locus_tag="PLO35_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79204.1"
                     /translation="MIRTLFIAIMVLLSCIVAPLFSQTAVYDVVPAATPLTATYNDGF
                     NMGSMSASGGTWWGVGSLVAGCCVGGVMPVSLGWGVPLVSAAAGSIPIAVAAAGNPQP
                     QPVMLMTLEQKGPDYINGFRAGYSQAQKKKNVSSAAIGMGISVALAVVAVLIIENAPE
                     DDELKWAP"
     gene            complement(8171..8827)
                     /locus_tag="PLO35_05745"
     CDS             complement(8171..8827)
                     /locus_tag="PLO35_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79205.1"
                     /translation="MRRIDWIILAVLAIALGVGGTVLYKTFTAPMETPASETEALDLK
                     PDPIQSDATGHGLPVISMGGRQIFLWAQAKYSITGQLVSKNRYTNGFMSDLSPWDYAL
                     AWGDVGTYLDQLRFKQMVRFCLFQPKKDAVVDFNYVNTHMSNNHLIPASKNVRKALSL
                     PKKGDIVKLDGFLVNVDAAKNGQTVATWNTSTTRSDTGNGACEIVYVTRLQIGERVFE
                     "
     gene            9118..10452
                     /locus_tag="PLO35_05750"
     CDS             9118..10452
                     /locus_tag="PLO35_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79206.1"
                     /translation="MKRGLFVLFVLAMLSLGSLVWGQNNALFFDGVNDCVAIGNMQSQ
                     ITTTYTVEAWINTKTVAVGSGELATYGRTIIASSSGNGKPLWVSLRGNQILVRSFNGN
                     NAQITYTIAGMTIDTWYHIAVSATRSGTVRLYVNGLPVASASAGTQFVWNNTMCIGDL
                     RPGRQVAFWGSIDDVRVWNDIRSDAEILANFDVPLSAPFDANLVGYWMLDESAGTTAY
                     DSVNNFDGTLRNGTSVQPTPQWVDGNPTLPIELSSFSATITSQYFVQLHWITQSETDV
                     MGYIVLRNNHNDLTTALQVSPLVEATNTTTQVSYSYTDSEVTPGIWYYWLQSIDLNGD
                     HQYHGPVMATVSEDSGSSAPQPAVINGINKLYPNPFNPSLNISFSLDKTADVSFKIYN
                     SRGQMVHSNVLGNRDAGTYNFVWNSGDLPTGFYLIRMQANNKVYTQKATLSK"
     gene            10614..12359
                     /locus_tag="PLO35_05755"
     CDS             10614..12359
                     /locus_tag="PLO35_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOH79207.1"
                     /translation="MITVHINGKATSVAKNTKVLHACAKAGYPVPHLCYHEDLPAFGN
                     CGVCMVEVNGRALRACSTPCEEGMQIKTTGKKLLDLRRDALEIILSNHPNNCPECVKN
                     GRCELQDLAQELAIRHMNLKKVRRKYKGRDESSPSITLDPSYCINCGRCTYVCCEIQD
                     VHALESSERGFNTYVGPTFNRPLEESECVKCGQCSAHCPVAAIYEVDDSDPVWKALDD
                     PDMVLVAQEAPAVRVALGEEFGYKPGTNVVGKMYTALREMGFHYVFDTNFGADLTIME
                     EASEFVEIFTKHPDHFPLITTCCPSWVDYLEKFHADLIPHFSSSKSPHQMVGTMVKTY
                     WAEKMKIDPTKIFLVSVMPCTAKKYEIERMEDMYASGHKDVDLTITTRELARMLKTRG
                     IDLAKLPDGQADNPLGEYSGAGTIFGATGGVMEAALRTAYYLATGTELPDPRIEFVRG
                     GLGIKKGKINILGTEVRIGVASGLGNVNKLMEEIRSAKAKGDEPPYHFVEVMACRGGC
                     VGGGGQPYRSTNRVRVARAKGLYKEDEGMTHRESHNNPSITKLYKDYLGKPNSAKARK
                     LLHTSYIARPMKIVK"
BASE COUNT         3028 a         3104 c         3274 g         2864 t
ORIGIN      
        1 cagagaaagc ggagaaagca gagaaatcag aggtttgata aacaatgaga tatgataaaa
       61 tgagagccaa attggaatgg caaacgtctc gtttgcctgc ttggtctcgc ctagagacta
      121 tctcatcaaa ggaacagagt ctattgatta gcttagagaa atccctcttt ttcccctttt
      181 tccctttttc tgcgttctat atgaaactca aaacaaacct cagagaaagc ggagaaagca
      241 gagaaatcag aggtttgata aacaatgaga tatgagagaa tgagggttca tttggattgg
      301 taaaagtcct ccaatggcat gcccgttctc gcgtaccgcg taaccagtaa ccagtaacca
      361 gtaacctgta accataaaac cgcattcgtg ttatcctgct taatcacacg cagtagagat
      421 gccgtggccc ctccggcgaa aatccccgga gagcgcacgg catcttcacg gcgggtaata
      481 gggaagctat atgggaattt gaacctgaaa cacgatacta acggactgag atgttttcta
      541 actatcactt gatacttgag cagaacttga tcatgatctc aactgaccca tagcccattt
      601 ttgagctttg ggtggggcat ggttggactg agaatgaact atgtctatag gggacaattg
      661 attatttggg tcagatcaat tatcatgtgt caaagtatgc tggcagaatt agtatgtctt
      721 ttgtaagtga tttgaaaaca gaaggtggaa tcatggcagt gaactacata aaaaacctgg
      781 aagccggcaa agcattgaaa ctggaagcac ttgtgcaata cagtcaggga cagatcgtga
      841 gccggaccct ggcccagaat aaagctgtaa gtctcactct cttcgcgttt tggcaaggag
      901 aggagatcag cagccatgat tccaaaggtg acgcgctgat cagcgttttg gatggagaag
      961 gcgagatcac gatcggggac cagacccata tggtgaaaaa aggtgaatcc atcctgatgc
     1021 cggctgagat tccccatgcc gtgaaagcag ttacaaactt caagatgtat ctgacggtgg
     1081 tgtttccgga tcaatgacta tttgctttca cggtattcaa agtgaatctt agcgctatct
     1141 gaactgccgt cctgatggga ctctgatggt cgagggatct cgtggttcta tctgtactgt
     1201 cttcccgatg ggactcaaag cgccaattat tcgctggctc ttaagccagt gatactaatc
     1261 aagctacagc tatattctgg cagaatagtt tacagcaggg tgggagctca gacgcggaca
     1321 ggcttattcc gggtgaggaa taccagcaga ccgatcaagg cggggataat atcctggact
     1381 acaaagaggg tgagggtggt ggagatggct tgcgcggcgc taaaaccagc ttcaccaagg
     1441 aaatggacgg cgaaactttc acgcagtccc agtccggcca gcgtgatggg aatgctgttt
     1501 gagaagttgg tgagggacat gagcttgagg ctctcgggcc aggaaatctg aatgtgggta
     1561 ttcaggatca gccagtactg gctgtaggcg ataagtgcag cgcccacttg cagtaaggcc
     1621 atgcgaggtg cgagctgttt gtacgctggc agggcttgac gtccttttgg ccaggctttc
     1681 aggatgggga tgcccaaaac gggggctacc aaacagagtg taaagacgat caccctgagc
     1741 cacaagggta tagctgtgaa ataaaagagc gccgcacccg aggcaaagct ccaggtggcc
     1801 caggtgagga agccgcgctc gattccgaag gccagcaccg aagccatgtg gctggaatta
     1861 tccacccaga agaccttccc caggctggct ggtccgcccg gaacggcaaa acgcaaaggc
     1921 aagccgatca ggtaggactt cagcacgtcg cggtcgttca ttttgtagga gggattgagg
     1981 ctgagggcgt ggcgccagtt gcggagctgg gcccaatgcc gcaaaatgga acctccgcag
     2041 aggacaataa acatccagag gggcagtctc agcatggatt tcagcaactc tctcaagtct
     2101 atgtctttaa agactatata gagtatgtac gcggtgatga ggaccttgag aacgtagagc
     2161 agggtcttga tcagagggcg ttttagccgg ttctgcagtc ggatggatat ctgctcttta
     2221 ggtggcattc tgccgcttca gtcctcaatt ccacaagcgg ccaaagcctg gttgataagg
     2281 tcctggggcg gagggctcaa atagccgtct tccatcatct tatgagtgag agagcgggaa
     2341 gcggtgatgt cctccatggc gatcttgaac acctcttctc tgctaagctg aatctgggcc
     2401 actccatcgg cgatggcttg catggccacg tcagccgcct cgtgggcaaa gacttccgtc
     2461 tcgtccatgg tgggaacgat gctttctgga tcaatgccgc ggttacgtgc aaagagagcc
     2521 agagaatggg ccgcggcgat ggccatggag tctgtgatct tggtagccct cactgtgagc
     2581 gcgcctttga ggataccggg aaaaccgcag gaattgttga cttgattggc aaaatcgcct
     2641 ctgccggtgg caactatata cgcgccagcg gctttggcat catagggata gatttccggt
     2701 acgggattgg cgcaggtgaa aacgatcgcc ttgggggcca tcagcctgat ccagtcaggt
     2761 ttgatagtat tgggaccggg tgtggaaagt gcgatcagca agtcagcgtc ccgcatggct
     2821 tcttccatgg tgttgttgcg caggggattg gtattctgtg ccaatgccca ttgtttgtag
     2881 cgagtgggat ccatctcaag gtctttgcgg cccagatgaa tgccgccctt gctgtcgaaa
     2941 acggtaaggc gggcagggtc taaaccgctc tgcaagagga agctggcgat ggtggtgttg
     3001 gaggccccgg caccaaagag caccgctttg atgttgccca tagtcttgcc ggtaagctcc
     3061 agagcgttga tcacgccggc cagagtcaca caggccgtac cctgagcgtc gtcatgccag
     3121 acggggatct ggcaggcctc gcgcagttcg tccaaaaccc tgtaacaatt gggctgggaa
     3181 atatcttcca ggttcacggc ccccacgctg ggttccagca ttttgacaaa ttctatcaat
     3241 ctggcgggat cgggtttgcc attggcatcg cggttattga cacagagcgc gatggcgtcg
     3301 cagccgccca aatannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacggtc
     3421 tgcatcttgc ctctgaaatg cgagtgcgtg agttccgaga gctttttgag aaaagccgtc
     3481 tgcgctccgg ctacttgggc ggccggaact ttggcggcaa aaaattcctt catgttgctg
     3541 aggtcctggc ggagttgggt cattttatct cccatgtacc gtttttattt gaacccttac
     3601 gggtcaccaa tcccatggct ttcaatctct ggagatgcct tttcacggtg gaggtgctca
     3661 tcccagtatg ttgagcaagc tgatcatagg taatcccggg gttcgcggcg agtaaggtca
     3721 ggaattggtt tattgggtca tttattgggt catttattgg gtcactggat agagtaccct
     3781 tgctggggcg ggtctttcct tcagacttca taggtttatc agtcacatcg attacgtgct
     3841 ctggtgattt gctgagaata gttgatctca gataatcgtc agtagcttca aagtgaggct
     3901 gggtattgtt aaactctttc aggatcggga tcactttggt gcgtattccc attccccggg
     3961 catccacata ttggtaatct ctgagtactt ccatgatcag ggtgtttctg ggagtgcgtc
     4021 ttcctccgat catcttttcc acggtcatcg aattgggcaa ggctcccggc ctaatatcgt
     4081 cacgcttgaa ctcgatttct gagttctcac cgtttttgat aatctcaagt agctctttct
     4141 ttagcatata atcctcttta ccaacttcaa actgctcttg tttgggtcca actcatgttg
     4201 cgtttttttt gcaggctgtc gccatgctgt cactcagccc gattctgtca agcctggcaa
     4261 atggagcttg acagcaaagg ccttctggag gggatggaac catgaaaaag acaatatcga
     4321 tcatcggagc cggactggcc ggcagcgaag cagccctcca actggcaaag cgaggttggg
     4381 aagtggcgct ttacgagatg cggccggggg tgatgactcc agctcatcag actggcctgg
     4441 cggcggaact ggtctgcagc aactcactca aatccactcg attggataca ggagctggaa
     4501 tgctcaaagc ggaaatggca ctgctgggct gccagctctt gcctctggcc gagaagtgca
     4561 aagtgcctgc cggtcacgct ctggctgtgg accgtcagct cttttccgaa gcggtggaag
     4621 atgccctcaa agcgcaagac tccatcaccc gtgtgaatga ggagatcaaa gaccttccgg
     4681 atggacctgt gatcatcagt gccggtccgc tggccacgga taatctgatg cgctcgctca
     4741 gtgcccttat cggtgatcaa catctctatt tctttgacgc cattgctccc atcgtggatg
     4801 ccgccagcct ggatagcggc attgtctata tgaaaggacg ctatgacaaa ggcgaggccg
     4861 attatatcaa ctgccccttt accaaagagc agtatctgga ctttgtggag gccctcctga
     4921 gcggcgaaca gcaccaggcg catgagtttg agaacgagtt ttttcatgat cccaagttta
     4981 gctactacga aaactgcacg cctatcgagg aactggccag acggggagtg gacacgctgc
     5041 gccatggagt gatgaggccc atgggcctgg aagacccgcg cgggggtggc aaaccctacg
     5101 ccgtcctgca gttaagagca gaaaataccg atttgaccgc ctataacctg gtgggatgtc
     5161 agaccatgct gcgttacggc gagcagaaaa gagtgttcag attgatcccg ggactggatc
     5221 aggcggaatt tttgcgctac ggctccatcc atcgcaacgc ctatctggat ggtccgaaag
     5281 tatttaacgc ggatctatcg ctccaggccc gtccggatgt atgggtggcc ggccaactga
     5341 gtggagtgga aggttatgtg gagtgtatcg ccagcgcttt gatcgtgagc aggatcatat
     5401 cagaggggct ggcgttgctg ccggaaacca gtattttggg ccagctttgg cggcgtctga
     5461 tcacccctca ggaccgcaaa tttcagcctg tcaatgccaa ttttggcttg ctgcctgatt
     5521 tggacagcaa gataagagac aaaaaactta agaaagagct tatggcccaa cgcgggctgg
     5581 aagccatgca agcatatatc gatcaaggag aaaaagatga ataaatacct atccgcctgc
     5641 ggactcgtct gtcccgattg cgaatgctac aaggccaccc tgagtggcga tctggcccaa
     5701 aaacaggata ttgctgagcg ctggagtaaa aactatcagg cctccctgac cgtggatgac
     5761 atccactgcg aaggctgcat gagctctggc cgcaaattca gctggtgcga taaatgtccc
     5821 atccgggcct gcgtagtgga aaaagggtac cagagctgtg ccgaatgcga cagcttcccc
     5881 tgccagacca acgcttttct ctatgagcat gtgccggcgg ctaaagagaa tatacttagc
     5941 ttgaggtgag aaacaccgcg cgcggttgaa atctctttaa acttgattgt ggctttctca
     6001 gtcagggaga atcagcaata gtcccaggag cgcttagaag ctcatccacg tttatcttaa
     6061 ggaattcatc caggattgcg gctttgtaaa tcgctctatc ctttgctgcc gcggaagcgg
     6121 aaccatagat attgccaagg taaaagccag taaaaaccga aaacactatt ccagccgacc
     6181 attggagatc attctgatag agctcgatac tggatatcag caaagccgaa ttgattacca
     6241 gggacgcaat ggccgtctcg aatcggcccg tgacagcgta acccagtccg ggtaagatgc
     6301 caaaagctcc tgctaataga gggcttgggg tgtgaggctt ggtggttttc tgtcggattc
     6361 tgagtgcttc ttcggccaga cgagctgtcg cactgtcaga taagctcaac tctcgcagtt
     6421 gagtaaccgc ctcttctgat tttcccagat actccaggct cagccccctc agcagctttg
     6481 ctctgggatc ggcagaccta tccagaaggc tgtaggctgg cagataatat ccatccctga
     6541 tcatcaagag ggcgttgtat ctggcagcca gatctgcagg tgtgagatac ccggcatatt
     6601 ggtgagaaac ttgaaacata ccagctctgt cggaagcctg aaacagtgat cgcatcaaca
     6661 tctgcacggc gtaaaggctg tccgctgagt tgtcagcttc ctgatacaga attcttttat
     6721 actcagtagc agcggcagtg taatcaccct gggaatagag ctgatcggca aacttgaggg
     6781 cactggcatg gcttaacccc aaaccaagca gcaatatagc cggcatcaag acgcgaaacc
     6841 atcgggcaag gtagtcaagg taactggtca ttgttcctct tcacttcatc caagtgcagc
     6901 tcagctcctt caatccagat ggaaggatat agatagccgt cccttccgca tctgatcaag
     6961 cggtcaaaag tcataagacc cccttttata gccccatatt gatccaccgc ttgagccgag
     7021 tagctggaac aaccagggta catggtgcat ctttctccat cgacggggga gatgtaatcg
     7081 cggtaaaagc ctattagcga agcataatca gaacttggca ctactctcat tgactgcata
     7141 gggtcattct ttgcgcacga aactccacca gttagagcaa ttgcaatgac aactgcagtt
     7201 agcatggatc tcagatgtct cttcattaaa ctcctgtcaa gcgtgcttac caaaattgat
     7261 ggcggccaaa cacagcaata gccgattgag aacgaaaaaa cagctcagcc tgaaaaaatg
     7321 ttctttgacc agggctgcgt ttaagaaact gggtggattg tatgaatgag ttaagcgatc
     7381 agggggccca tttgagctca tcgtcttctg gagcgttttc gatgatcaaa accgctacga
     7441 cggctaaagc cactgaaatt cccatgccga tggcggcgga ggacacattc ttcttcttct
     7501 gagcctggct gtatccggcc ctgaaaccgt ttatgtaatc cgggcctttt tgttccaaag
     7561 tcatcagcat gaccggttgc ggttgtggat tgccagcggc ggctactgca attggaatgc
     7621 ttcctgcagc ggcagaaacc agtggaacac cccagcccag actcacaggc attacgcctc
     7681 ccacacagca tccggcaacc agagaaccaa ctccccacca agtaccaccg cttgcggaca
     7741 ttgaacccat gttgaagcca tcattatatg tggcggttaa tggagtagcg gcaggcacta
     7801 cgtcatagac tgccgtttgt gaaaaaagcg gcgcaactat acaagatagc aacaccatga
     7861 tagcgatgaa tagtgttcga atcatcactt acctccagaa atagaattat ccaatcgatc
     7921 agattgaact atgaacaccg tatctgggag aacaatttat gtcaagccaa ttctggactc
     7981 aggtaaaaaa cgtttatacg agcagggttg cagataaacc atcacaaccc tgccactcta
     8041 ttgaatagca tcctcagcag gatattgcca acggggagga ttatatctct gtggctccgg
     8101 ttattttgtg gtgggtgagg tacttatggc aggcttaaaa tgaggtcagc gacatcgcat
     8161 gattacgaga ttattcaaac accctctctc caatctgcag cctggtcaca tagacgatct
     8221 cgcaggcccc gttgccagtg tcgctgcggg tggtggacgt gttccaggtg gccacggttt
     8281 gcccgttttt ggctgcgtcg acgttcacta gaaagccgtc cagcttcacg atgtctccct
     8341 ttttgggcag gctgagagct ttgcggacgt ttttggatgc ggggatgagg tggttgttgg
     8401 acatatgagt gttcacatag ttaaaatcca ccaccgcgtc ctttttaggt tgaaagaggc
     8461 agaaacgcac catctgtttg aagcggagct ggtccaggta ggttcctaca tctccccagg
     8521 ccagagcata gtcccagggg gagagatcgc tcataaaccc gttggtatag cggtttttgc
     8581 tcaccagctg gccagtgatg gagtattttg cctgagccca gagaaagatc tggcggcctc
     8641 ccattgagat gacgggtaag ccgtgaccag tcgcgtcgct ctggatggga tccggtttca
     8701 ggtccagcgc ttcggtttca gaggccggag tttccatagg agcggtaaag gttttataca
     8761 acaccgtgcc gcccacccca agcgctatgg ccaaaacggc cagaattatc cagtcaattc
     8821 gtcgcatccc agtccttaat tatgtcagag tcttacagct aagacaatcc tggcggccgg
     8881 acgactgctg tcaagcttta atcgtttgaa gaatctaaac agcagctctc atggttgatt
     8941 tgatgccctg attggggttt agtgccatgg caccgtcaca atccaaagca ttaagaatta
     9001 accggaatct gaaaaaaaag gcttgacgaa tttggtgcct gccatataaa cggcgcatca
     9061 ggtgtaagtg cgcctgtaag tatcgaaaat agcgtgaacg acatcaagga ggatatcatg
     9121 aaacgcggat tgtttgtttt atttgtgttg gcaatgttgt cactgggttc cctggtttgg
     9181 ggccaaaaca acgcgttgtt ttttgacggt gtgaatgact gtgtagcaat tggcaacatg
     9241 caatctcaaa tcacgacgac atacacggtg gaagcatgga tcaacactaa aacggttgcg
     9301 gtgggaagcg gagaactggc cacttatggc aggacgatca ttgcttcttc ctccggtaac
     9361 ggcaaacccc tgtgggtgtc tcttagggga aatcagattt tggtaagatc ttttaacggc
     9421 aacaacgctc agatcacata caccattgcc gggatgacaa ttgatacctg gtatcacatc
     9481 gccgtatcag ccacccgatc cggcacagtg aggctttacg tgaacggctt gccggttgct
     9541 tctgcttccg ccgggaccca gtttgtctgg aacaacacca tgtgtatcgg tgacctccgt
     9601 cccggcagac aggtcgcctt ttggggctct atcgacgatg tgcgggtgtg gaacgacatc
     9661 cgcagcgatg ctgagattct ggccaatttc gatgttccgc tcagcgctcc ctttgatgcc
     9721 aatctggtgg gttactggat gctggatgaa agtgccggca ccactgccta tgacagcgtc
     9781 aataacttcg acggcaccct cagaaacggg acctcagtgc aacccactcc gcaatgggtg
     9841 gatggcaacc cgactctgcc gatcgagctt tccagcttct cggccaccat tacttcgcag
     9901 tacttcgtgc aattgcactg gatcacccag tccgagacag acgtgatggg ctatattgtg
     9961 ctgcgcaaca accacaacga tctgaccact gctttgcagg ttagccccct cgtcgaggcc
    10021 accaacacca ctacccaggt ttcttacagc tacacagaca gcgaagtcac ccccggaatc
    10081 tggtattact ggctgcaaag catcgacctc aacggagatc accagtatca cggtcctgtg
    10141 atggccaccg tatctgaaga ttccggcagc tcggcacccc aacccgctgt gatcaatggt
    10201 atcaacaagc tgtatcccaa tcctttcaac cccagcctca atatctcgtt cagcctggac
    10261 aagacggccg acgtgagctt caagatttac aattcccgtg gacagatggt gcacagcaac
    10321 gtccttggta acagagacgc gggaacctac aacttcgtgt ggaacagcgg tgacctgccc
    10381 accggattct atctgatccg catgcaggcc aacaacaaag tgtacaccca gaaggcgacg
    10441 ctctccaagt aagccgattt tcctcctgat ttcatacagg gtccccggtc ctgcagattg
    10501 ggggctcttt ttttatggcc aaatatccca aacctgtctg aattctgtgc ttgacagaaa
    10561 aagcagttta tagacaggag attttcatca aaacatcccc ctgaggagag ataatgatca
    10621 cagtccatat taacggcaag gccacctccg tggccaaaaa caccaaggtt ttacacgcct
    10681 gcgccaaagc tggctatcct gtcccgcacc tctgttatca tgaagacctg cctgcctttg
    10741 gcaactgtgg agtctgcatg gtagaggtca acggacgggc tttgagggcc tgctccactc
    10801 cctgcgaaga gggcatgcag atcaagacca ccggcaaaaa actgctggac ctgaggcgtg
    10861 acgctctgga gatcatcctc tccaaccatc ccaacaactg tcccgaatgc gtcaaaaacg
    10921 gtcgctgcga gctgcaggac ctggcccagg aactggccat ccgccacatg aatctcaaga
    10981 aagtgcgccg caaatataag ggccgcgacg aatcctcgcc ctcaatcacc ctcgatcctt
    11041 cctactgtat taactgtgga cgctgcacct atgtgtgctg cgaaatccag gatgtccacg
    11101 ctttggagag ctccgaacgc ggcttcaaca cctatgtggg ccccaccttc aatcgccccc
    11161 tggaagagag cgaatgcgtg aagtgtggtc agtgctcggc ccactgtccc gtagccgcga
    11221 tctatgaggt ggatgattcc gatcccgtgt ggaaggcttt ggacgatccg gacatggtgc
    11281 tggtggccca ggaagctcct gccgtgcggg tggctttggg cgaagaattt ggttataagc
    11341 ccggcaccaa cgtggtgggc aagatgtata ccgccttgcg ggagatgggc ttccactatg
    11401 tgtttgacac caatttcggt gccgacctca ccatcatgga agaggcatcc gagtttgtgg
    11461 aaatcttcac caagcatcct gatcactttc cgctgatcac cacttgctgt ccctcctggg
    11521 tggattatct ggagaagttc catgccgatt tgatcccgca tttctcctcc tccaaatccc
    11581 ctcatcagat ggtgggcacg atggtgaaga cctattgggc cgaaaagatg aagatcgatc
    11641 caaccaagat ctttctggta tcggtaatgc cttgcaccgc caagaaatat gagatcgagc
    11701 gcatggaaga tatgtatgcc agtggccaca aggacgtgga cctcaccatc accacccgag
    11761 aactggcccg catgctcaaa acccgcggca ttgacctggc caaattgcct gatggccaag
    11821 cggataatcc tctgggagaa tacagcggcg ccgggacaat ttttggagcg acaggcggag
    11881 tgatggaagc ggcgctcaga accgcctact atctggctac cggcacagaa cttcccgatc
    11941 ccaggatcga attcgtgcgc ggtggcctgg gcatcaagaa aggcaaaatc aatattctcg
    12001 gcacagaagt ccgcattgga gtggcttccg gacttggcaa cgtcaacaag ctcatggaag
    12061 agatcaggtc cgccaaggcc aaaggcgatg aaccacctta ccactttgtg gaagtaatgg
    12121 cctgccgggg tggctgtgtg ggtgggggtg gacagcccta ccgctccacc aaccgcgtca
    12181 gagtagcccg cgccaaaggc ctctacaaag aggacgaagg catgacgcat cgtgagtccc
    12241 acaacaatcc ctccatcacc aagctctata aagactatct cggcaagccc aattccgcca
    12301 aagccaggaa gctgttgcat accagctata tagcgaggcc gatgaagata gtgaagtgag
    12361 atgccttcgg
//
LOCUS       DAOSFN010000069        12333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4313_length_12333_cov_9.244502, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000069 DAOSFN010000000
VERSION     DAOSFN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 12333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12333
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4313_length_12333_cov_9.244502"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..5041
                     /locus_tag="PLO35_05760"
     CDS             320..5041
                     /locus_tag="PLO35_05760"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOH79208.1"
                     /translation="MTRKMTLLLLVLAGLLCVGLRAEEVTVGSGADLASVPVDMYYRN
                     SLFECLFYNGDLGFENGTITALKFYNSFVSNLSNKPIRIWLGGTTLTNLSAGWIPSSQ
                     LTLVYDGAVNFPSGSNIIEIPLTVPYTYTGGILAMLVQRPMDGNFYSVSDRFQAQTVG
                     TIRARKAQSDSNVLDPANPSAPGTVSGTFPQTTFVYSLTALANDLVCESVSGDQSPSV
                     GNPTAYQVRIRNNGSQSQSVYSVRLMKQGGVELASLPGQSIAPQQTLDLSLEWTPDLA
                     GPTSIYARVELAGDQALLNNQSLSLDLNVQPEGTYSVTVGSGNENARLPIDFYHSASL
                     CETIFLAPEINTNGLLHGIKLYNNFIVNIPYQLIYIWMGETNLADLSSGWVSANALSL
                     VFSGTVDFPAGHNEIVINFTAPFHYAGGNLVMMIEHPPGSQYSVNENYFATQSGGLGP
                     RSRMVSGSGIDYNPDSPPAAPTTPSFPKTSFLFIDNGLGSLSGTVYGPNGLGLGGATV
                     NVIGNSLSCLTSINGSYSFPHVSAGSYQVRAQKHGYESEDLPVTIMEDQASILDFNLD
                     ALPSVNVTGRVVVNGDPNSGIAGAGVTLEGFYNYQTLTDGLGYFSFPAVIQNHSYQYD
                     VSKTGYQNATGVIAVGTQDLNMGNIMLIEIAYPPSDVMASIAGGQVNLSWTGPAAVNG
                     QWVHYDNGANLTAIGMGGVANFDVAVRYPASALVPYTGMSLRAVRVWVASEGSFTIKV
                     WRGGSSAQPGQLWTTQIFTPVLDAWTTVILSNPVPVTGQEELWFGYNCNVYSGFPAGC
                     DSGPVADGLGNLIWYQNAWGTLLQLSDQLHYNWNIQGYLSFTGFPEAVGNAPQISKGT
                     EAPSGRSLNGYQVWRLAQGQESNEQLWTLLTPEPVTSTSFIDSGWGQLEDGIWTWAVK
                     ARYSGGVYSLPAFSNGLAMYNQSGFIAGLVRSEYALPIQGASVTCGPVTVSTDANGAF
                     SILVPSGTYSVTASHPQYVPVTQSGVQVTVGNTTTLYFALNGAQTLFSDGFEDYSSWS
                     LNFPPWMTVDHDLSPTYSLPATVWPHINEPQAFIVFCPVGAVPPAGSVIPHGGIMVAA
                     CFASTDGPNDDWLISPQLNGVTRFSFWAKSHNSQYGLERFKLAVSTTGPVPTDFVAIS
                     GPDPIEVPAVWTRYEYDLSSYGNTYIRVGINCVSDDAMALFIDDVSFIGQAVQGFSQN
                     INLSSGWNLVSLNVAPVDHSVAALISEIAGSVVQIKGSEGVYIPGNPYNTLNQFTDGR
                     AYSIKMLSPAVWVVSGNQLSLATNIVLNQGWNLAAYLPQQSMPASTATQGIADWLLEM
                     KGTQGIYIPGNPYNTLTSMQPGQGYWMKLSEPRVFNYPNPQPSQSSPGQADKDGEPVV
                     LPSSMTVLARCDFASEGDILQAWVGDELRGQETLIAPEAFAACLIQIYTEIPGEQVRF
                     RILAPEGELPVQTVIYSLPGSSLGEYPQFVVLEAGSSGSADEIPIPTRLNAVWPNPFN
                     PSAQIGFSVGRDNLPLRINVYNLRGQKVITLADQSYSRGSHSVCWDGRDAHGKPVSSG
                     VYIIELQSPGIRMSAKAVLAK"
     gene            5085..8405
                     /locus_tag="PLO35_05765"
     CDS             5085..8405
                     /locus_tag="PLO35_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79209.1"
                     /translation="MKSRMLFIMALLMAVGSLRAEPMPCSQGFQAIDMPQIPGPLAER
                     VPPDYTFTRTPVPIMNSYYDYMIGSYNNLPLRVIPASAEGGYFLTHHGSRASTSLRRV
                     FYTYLDASGVLINNIEINPYNIQEGYPSLAVDPLTGKPMYAWHANADTDTALETQFVA
                     DAFMAGISGLFNDIQVIHNNPITITAPNGTVTTDNQFIWPTAQVGPSPIAGKRRVYVL
                     ARNSVSHSPGESPNVRIGYADFDGDNLENGIPLVWSYTHIPMLDQWNIDQIWRQPFLA
                     LAVDNAGNLYYIGYHTTWDQNGYVSEEDIDVFRCDNYGQGTWSRQSFWGHLPTWNPPS
                     VPGGNSGYFTNGSGVPYTNSQLFFKLMNSGHLNAGLDALGRIHALGSWALSNSDFGYY
                     PELQFMKDLIYDPGAQQIQIREVYPRKDVNDLTNSCYTPWDLQYPYGQVDAYDAEGPV
                     MSGDWPFPHWDATAHEDKMMLLYNNPKITNANSEGMMAAVWQSSQRARNFNYYSDGQY
                     AAYANAPEICISVSGDNGNTWSDPIVLNKVDTPQFANLKPMWAYPADQIIYTGMQGNH
                     RVGKLGLMFYDDNTWGANVISPPYHPSPDGGRIMFCELQIQFPVQSNPTDPFGTPLVL
                     DHSMQVRASFQSSGVAAQAGDVLAAFVKVNNIPQLRGKATVYQQDNVSHANLNVACLS
                     DDELVYFKLWDASSSSVYLIAETLLTQINGSVGVWPNDPFWVHTIIPGQQVLELQSGW
                     NMSSVNVHPVNQNISNVFGGIMPLVQMIKCPDGIFVPNNPYNTLTDLTDGKGYFIRLS
                     QPGSLYLNGAQMEPGTAIPLLEGWNLVGFTPIIAQSVDHAVSSLGPYLLQIKGAEGIY
                     IPGNPYNTLNSLAPGRAYWMLLSQPYSLFYPNPLRDAIPQEILSCPQYGSPMIKPQSQ
                     AILTRLGTYAAEGDLLAAFVGDELRGLSSVKAVDNSLGSLMQVFTESAGEELAFRLLK
                     AGSDAEVELLPGLSSAPGTTLGDYQSGSYLDLSPSQDIPPVQSTRLISAYPNPFREGC
                     AIKLQVAKDAPAISLEIYNLRGQKVACVYKGQLSQGLHSLVWSGLDDTGRKLGSGIYL
                     CRLNTGKSSQNLKLMLVK"
     gene            8417..11059
                     /locus_tag="PLO35_05770"
     CDS             8417..11059
                     /locus_tag="PLO35_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH79210.1"
                     /translation="MRLIPILLALLLLAGGLHAIDPWGEPLILTGSMTVMAQVRINGF
                     ATTPGDVLAAFVSVNGVPELRGKQAITVNSNISGCLIQIYTDANDEEIRFMVWDESSQ
                     QVYVCTQALASMVNGIVGSWPSNLYQINVSIPLNTDPWGAPTILSSSMSVMAQVSVNG
                     SGAEWGDILAAFVSIDGTEHLRGKTPILINDGVSGCLLQVYTESSGEPITFKVWDANT
                     QTIYVCPQTLSSVVNGSVGSWPANLYQIDASQPALTDPWGIPTVLSGSMSVMARVLIN
                     NQDASNGDVLSAFVDVQGTEQLRGKATVAINANEPGCLIQIFTDSNDETISFKVWDYS
                     AQEIIPVNNILPSQLNAMIGGWPDNLYQINAGPGVHELTNPIFDPPDGYYVQSQTVSI
                     SCFIPGAQIYYTLDGSEPTLSSPLYTGPIAVNTSTTIKARAWLAGWSPSAISTAHYTI
                     TGQAEAPSFNPAPGTYTAPQNIVISAPGTGLTIRYTLDGSEPGPGSPAYVMPINLAQG
                     VHTLKARVWRPGWNPSPTTTGVYTITGTVAMPFVNPPGGTYTVAQTITISCSTPGSQI
                     RYTTDGSEPNDNSTLYTGPFTVSSSLTLKAKGFKADWAPSQTASVVYTITGTVAMPSA
                     DPPAGVYTQPVTVSLSCSTPGAVIRYTTNGSNPSVGSTPYTTPILIGSSTPLKAKGFL
                     SGWNASPTLTANYAITGTVATPSFSHPSGTYNRHLTFSIACATPAAQIRYTLDGTDPD
                     SGSALYTDSIYIGATTTVKARGYKTGWEPSEIDSVVYQISLANPDAPDTPLPPGITSA
                     WPNPFTRRITIAVRLKEASPVAKLGIYNLRGECVRSFEATFIGEYEFEWDGCDQRGRR
                     MPSGIYLLRLKSGRLAQTRKIVFK"
     gene            11074..12204
                     /locus_tag="PLO35_05775"
     CDS             11074..12204
                     /locus_tag="PLO35_05775"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOH79211.1"
                     /translation="MKHSLLTLAFLSVCCLILATGPQELVTDNPTRTVIFGDNFDSYP
                     NFATTFAPWTLVDQDLSNVFGFPGMTVPNIGNPMAYMIFNPSATQPPMTAVSAHSGTK
                     MAASFAATNPPNSDYLITPRLQLGNFSSLSFWARSHNNSFGLERFRVGVCVSLNPVPA
                     AFNWLSANYVEAPAYWTQYSYDLGAWDGQQIYIAIRCVSDYSTAFYVDDFNVMSDVAQ
                     PPQLIGLNAGWNLVSLRVSPSDHSIPTLIQPIQNFVEQIKGTEGVYIPGNPFNTLTQL
                     SDGKAYLIKMSAAANWSVSGFQIPTGTPLPLTSGWNLVAYLPQSSMAVNIATQSIESW
                     LLAVKGSDGMYYPGNPYSTLTTMYPGKGYWMRLSEARSLVYP"
BASE COUNT         2711 a         3815 c         3265 g         2542 t
ORIGIN      
        1 tttttcatat cttattgtaa aatagcctct gctttctctg ctttctccgc tttctctgaa
       61 gcttgttgtc tggcaccccg gcaaaaaaaa gtgaaaatct gcgagattaa gcttgacagc
      121 agtatgaaca tcaataacgt tgacaaaaga tagaatgggt ggggattatc cagccaggcc
      181 ttgcgcaagc atcgtgaaag agactgagtt ccagagcggt acagagcatg gttcccgtca
      241 cggaaagctg gttgataccc aaaccccaga gctgcgctaa gacatagcga agcggacctc
      301 attgaccggg gaggtgacca tgacgcgaaa gatgacgctg ctcctgctgg tgctggcggg
      361 attgctgtgt gtggggctgc gtgccgaaga ggtgacggta ggctccgggg ccgatctggc
      421 ctccgttccg gtggatatgt attatcgcaa ttcgctgttt gagtgtctct tttataatgg
      481 cgatctgggc tttgagaatg gcacgatcac ggccctgaaa ttctacaaca gctttgtgag
      541 caatctgagc aacaagccca tccggatctg gctgggcggc accactttga ccaacctgag
      601 cgccggctgg atcccatctt ctcagcttac tctggtttac gacggggcgg tgaacttccc
      661 cagtggcagc aacatcatag agatcccgct cacggtccca tacacctaca cgggcggcat
      721 tctggccatg ctggtccagc ggcccatgga cggcaatttc tatagcgtca gcgatagatt
      781 tcaggcccag accgttggca caatccgggc ccgcaaagct caaagcgaca gcaatgttct
      841 cgaccccgcc aatccatccg ctccgggcac ggtgagcggc acctttcccc aaaccacctt
      901 tgtctatagc cttacggccc ttgccaacga cctcgtctgc gagagtgtaa gcggagatca
      961 gagccccagc gtgggcaacc ccaccgccta tcaggtgagg atcaggaaca acggctccca
     1021 gtctcagagc gtttacagcg tccgtctgat gaagcaggga ggggtggaac tggccagtct
     1081 gccaggccag agcatcgctc cgcaacagac tctggacctc agcctggagt ggactcccga
     1141 tctggctggc ccgaccagta tttatgccag agtggaactg gcaggagatc aggcgcttct
     1201 aaacaaccag agcctcagcc tcgacctgaa cgttcagcca gagggaactt acagcgtgac
     1261 agtgggttcc ggaaacgaaa acgctcgcct gccgatcgac ttttaccaca gcgcttccct
     1321 ctgcgagacc atcttcctgg ccccggagat caacaccaac ggcctcctgc acggcatcaa
     1381 actatacaac aactttatag tcaatatccc gtatcagctc atctacatct ggatgggcga
     1441 gaccaacctg gcggacctct ccagcggctg ggtcagcgcc aacgcgctca gcctggtgtt
     1501 tagcggcacg gtggattttc cggccgggca caacgaaatc gtgatcaact tcacggcccc
     1561 gtttcattac gcgggcggaa atctggtgat gatgatcgag catccgcccg gcagccagta
     1621 ttctgtgaat gaaaactact ttgccaccca gtccggaggc ttgggacccc gttccagaat
     1681 ggtctccggc agcggcatcg attacaatcc ggacagccct cccgccgctc ccaccacccc
     1741 cagctttccc aagaccagct ttttgtttat agacaacggc ttaggttcac tgagcggaac
     1801 ggtctatggc cccaatggat tgggactggg aggcgccact gtgaatgtta tcggcaacag
     1861 cctcagttgt ttaacctcta taaacggcag ctatagcttc cctcacgtct ctgctggcag
     1921 ctatcaggtg agagcccaga aacacggcta tgaaagcgag gacctgcccg tcacgatcat
     1981 ggaagaccag gccagcatcc tggattttaa tctggacgcg ttgccctctg tgaatgtcac
     2041 aggccgggta gtggtcaacg gcgatccaaa cagcggaatt gccggcgcgg gtgtaactct
     2101 ggagggattt tataactatc agacccttac ggacgggctg ggctacttca gctttcccgc
     2161 cgtcatccaa aaccactctt atcaatatga tgtgagcaaa accggctacc agaacgccac
     2221 aggagtgatt gccgtgggca cacaggacct gaatatgggc aacatcatgc tgattgagat
     2281 cgcctatccg ccctcggatg tgatggcttc catcgctggc ggccaggtaa acctgagctg
     2341 gactggcccg gcggccgtca atggacaatg ggtccattac gacaacggcg ccaatctgac
     2401 agccatagga atggggggag tggccaattt tgacgtggcc gttcgctatc ccgcctctgc
     2461 tctggttccc tatacaggca tgtctctgag ggcagtgaga gtatgggtgg ccagcgaggg
     2521 gagcttcacc atcaaggtct ggcggggtgg atcttccgct cagcccggcc agttgtggac
     2581 cacccagatc ttcacccccg ttttggatgc ctggaccacc gtgatcctca gcaaccccgt
     2641 cccggtcaca ggtcaggaag agctgtggtt tggatataac tgcaacgttt acagcggttt
     2701 ccctgccgga tgcgatagcg ggcccgtggc cgacggactg ggcaacctga tctggtatca
     2761 gaatgcatgg ggcactctgc tgcagctttc agaccagctg cactacaact ggaatatcca
     2821 aggctacctg agcttcaccg gtttcccgga agccgttgga aacgctcctc agatcagcaa
     2881 aggcacggag gctccttccg gacgcagcct gaacggctac caggtctggc gtctggccca
     2941 ggggcaagag agcaatgaac agctttggac gctgctcaca ccagagcccg tcacatctac
     3001 aagctttatc gatagcggct ggggacagct cgaagatgga atctggacct gggccgtgaa
     3061 agcgcgttac agtggcggag tgtattcgct gccagccttt tccaacggtc tggccatgta
     3121 taaccagagc gggttcatcg ctggcctggt gcgcagcgaa tatgccctgc ccattcaggg
     3181 ggccagcgtc acctgtggcc ctgtgaccgt cagcaccgat gccaacgggg ccttcagtat
     3241 actggtcccg tccggcacct atagcgtgac tgcctcgcat cctcaatatg tgcccgtcac
     3301 tcagagcggg gtgcaggtca cggttggcaa taccaccacc ctctattttg cgctgaatgg
     3361 agcgcagacg cttttcagcg atggatttga ggactacagc agctggagcc tgaacttccc
     3421 gccctggatg actgtggacc atgatctgag ccccacctac agtctgccag ccacagtctg
     3481 gcctcatatc aacgaaccgc aggcttttat cgtcttctgc ccggttggcg ctgttcctcc
     3541 agccggatcg gtaataccac atggcggtat catggtggcg gcctgttttg ccagcacgga
     3601 cggtcctaat gacgactggc tgatctctcc gcagctcaat ggcgtcaccc gtttcagctt
     3661 ctgggccaaa agccacaaca gccaatacgg tttggaacgc tttaagctgg cggtttccac
     3721 cacgggtcca gtgcccacgg actttgtggc gatcagcggt cccgatccga tcgaggtacc
     3781 ggctgtctgg acccgctacg agtatgacct cagctcctat ggaaacacct atatccgcgt
     3841 gggcatcaac tgcgtgtccg acgatgccat ggccctgttt atcgacgacg tcagtttcat
     3901 cggtcaggcc gtacagggct ttagtcagaa catcaatctg agctcaggct ggaatctggt
     3961 ttcgctgaac gtggcccctg tcgatcactc cgtggcggct ttgatctcag agatcgcggg
     4021 ctccgtggtc cagatcaaag gcagcgaggg agtttacatc cccggtaatc cttacaatac
     4081 attgaatcag tttacagacg gccgggctta cagcatcaaa atgctctctc cagccgtatg
     4141 ggtagtgagc ggcaatcagc tctcgctggc caccaatatt gtcttgaatc agggctggaa
     4201 cctggcggcc tatctgcctc agcagtccat gccggcatct acagccactc agggcattgc
     4261 ggactggttg ctcgaaatga aaggcactca ggggatttat atccccggca atccctacaa
     4321 cactctcacc tcgatgcagc ctggccaggg ctactggatg aagctctcgg agccccgggt
     4381 gttcaactat cccaatcctc agccctctca aagctctccc gggcaggcag ataaggatgg
     4441 cgaaccggtg gtgctgcctt ccagcatgac cgtgctggcc cgctgcgact ttgcctcgga
     4501 gggagatatc ctccaggcct gggtgggaga cgaactgcgg ggccaggaaa ccctgatcgc
     4561 tccggaagct tttgccgcct gcctgattca gatatatacc gagatacccg gagagcaggt
     4621 ccgcttcaga atcctcgctc cggaagggga actgcctgtc cagaccgtga tttacagcct
     4681 tccgggctcc tcgctgggag agtatccgca gtttgtggtc ctggaggcgg gcagctcggg
     4741 gtccgcggat gagatcccta tccccacacg gcttaacgct gtgtggccca atcccttcaa
     4801 tcccagcgcc cagatcggct tcagtgtggg ccgtgataat ctgccgctgc ggataaacgt
     4861 ctataacctc agaggccaga aagtgatcac cctggcagac cagagctatt ccagaggcag
     4921 ccacagcgtc tgctgggatg gccgggacgc gcatggaaag cctgtctcct cgggagttta
     4981 tatcatagaa ttgcagagcc cgggtatccg gatgagcgcc aaagcggtgc tcgccaaata
     5041 gattttgcgt ggcctcgcag atgtgaggcc gccaaggagc aaagatgaaa agcaggatgc
     5101 tgttcataat ggcgctgctc atggctgtgg gcagcctgag ggcagagccg atgccttgct
     5161 cgcagggctt ccaggccata gatatgcctc aaattcccgg cccgcttgcc gagcgggtgc
     5221 ctccagatta cactttcacc cgcaccccgg tcccgatcat gaacagctat tacgactata
     5281 tgatcggcag ctacaacaat ctgcccctca gagtgatacc tgcctccgcg gaagggggat
     5341 acttcctcac ccatcatggc agccgggctt ccaccagcct gcgcagagtc ttctatacct
     5401 atctggatgc cagtggcgtg ttgatcaaca acatcgagat taacccatac aacatccagg
     5461 aaggctatcc cagcctggcc gtcgatcccc tcaccggcaa acccatgtat gcctggcatg
     5521 ccaatgccga taccgatacg gctctggaga ctcaatttgt ggccgatgcc tttatggctg
     5581 ggatttccgg cctcttcaac gatatccagg tgatccacaa taaccccatc accatcaccg
     5641 ctcccaatgg cactgtgacc accgacaacc aatttatctg gcccacggcc caggtgggtc
     5701 cctcacctat tgccgggaaa cgcagggtct atgtgctggc ccgcaacagc gtgtcccaca
     5761 gccccggaga atccccaaac gtcaggatag gctacgcgga ttttgacggc gataaccttg
     5821 agaacggcat tcccctggtc tggagctata cccacatccc tatgctggac caatggaaca
     5881 tagatcagat ctggaggcag cccttcctgg ccctggcagt ggataatgcc ggaaatctct
     5941 actatattgg atatcacact acctgggacc aaaacggcta cgtcagcgag gaagatatcg
     6001 acgtcttcag atgcgacaac tacggccagg gcacctggag cagacagagc ttttggggcc
     6061 atctgcccac ctggaatcct ccttccgtac caggtggaaa ctcaggttac ttcaccaacg
     6121 gctccggagt tccatacacc aacagccagc tctttttcaa gcttatgaac agcggccatc
     6181 tcaacgccgg tctcgacgcc ctgggcagga ttcacgccct gggaagctgg gctctctcca
     6241 acagcgactt tggctattat ccggaactgc agtttatgaa agacctcatc tacgaccccg
     6301 gcgctcagca aatccagatc agggaagtgt atccccgcaa ggacgtcaac gacctcacca
     6361 acagctgtta cactccctgg gacctgcaat atccctatgg ccaggtggat gcctacgacg
     6421 cggaaggtcc tgtgatgagc ggcgactggc ctttcccgca ttgggacgcc acggcccatg
     6481 aggataagat gatgctcctc tacaacaatc ccaaaatcac caacgccaat tccgagggca
     6541 tgatggcagc cgtgtggcag agctcccaac gcgcccgcaa tttcaactac tactccgacg
     6601 gtcaatatgc cgcttatgcc aacgcgcctg aaatctgcat cagcgtgagc ggagacaacg
     6661 gcaacacctg gagcgacccc atcgtgctca acaaggtgga tacccctcag tttgccaatc
     6721 tcaagcccat gtgggcctat cctgccgacc agatcatcta taccggcatg cagggcaacc
     6781 acagagtggg aaagctggga ttgatgtttt atgacgacaa cacctgggga gccaatgtca
     6841 tctcgcctcc ttatcatccc agtccggatg gaggcaggat aatgttttgc gagttacaga
     6901 tccagtttcc ggtgcaaagc aaccccaccg atcctttcgg aactcccctg gtcctggacc
     6961 acagcatgca ggtgcgcgct tcctttcaga gcagtggtgt ggctgcccag gcgggagacg
     7021 tcctggcggc tttcgtaaag gtcaacaaca tcccccagct cagaggcaag gccactgtgt
     7081 atcaacagga taatgtctct catgccaatc tgaatgtggc ctgccttagc gatgacgagc
     7141 tggtctattt caagctctgg gatgccagca gcagttccgt ctatctgatc gcggagacac
     7201 ttctgaccca gatcaacggc agcgtgggag tctggcccaa tgatcccttc tgggtccaca
     7261 ccatcatacc gggccagcag gtattggaac tgcagagcgg ctggaacatg agctcggtca
     7321 acgtgcatcc ggtcaaccag aacatctcaa acgtctttgg cggcatcatg cctctggtgc
     7381 agatgatcaa atgtccggac ggcatcttcg ttcccaacaa tccttataat acactcactg
     7441 acctcacgga cggcaagggc tatttcattc gtctcagcca gcccggcagc ctctatctaa
     7501 acggagctca gatggaaccc ggcactgcca ttccactcct ggagggatgg aacctggtgg
     7561 gcttcactcc catcatcgct cagtccgtgg accatgccgt ctcctctttg gggccctatc
     7621 tgctccagat caaaggcgct gagggtatct acattcctgg aaatccctac aacactctca
     7681 acagccttgc tccgggcagg gcatactgga tgctgctcag ccagccctat agcctctttt
     7741 atcccaatcc cctccgcgat gccatccctc aggaaatcct gagctgccct cagtatggca
     7801 gtcccatgat taagcctcag agccaggcca tcctcacccg gctgggaacc tacgcggctg
     7861 agggtgatct gctggccgcc tttgtgggtg atgaactgcg ggggctcagc tcagtcaaag
     7921 cagtggacaa cagcctgggc agcctcatgc aggtgttcac agaaagcgcg ggtgaagagc
     7981 ttgcctttcg cctgttaaaa gccggttcgg atgccgaagt ggaactgctc cccgggctga
     8041 gcagcgcgcc tggaaccact ctgggagatt accagagtgg atcatatctg gacctctcgc
     8101 cctcccagga tatccctccc gtgcaaagca cccgactgat ctcagcttat cccaatccct
     8161 tccgcgaggg ctgtgccatc aaactgcagg tggccaaaga cgcgccagcc atcagcctgg
     8221 aaatctacaa tctgcggggc cagaaagtgg cctgtgtata taaaggccag ctcagccagg
     8281 gcctccacag cctggtctgg agcggtctgg atgacaccgg ccgcaagctg ggctccggca
     8341 tctatctctg ccgtctgaat accggcaaga gctcccaaaa cctgaaactg atgctggtga
     8401 aatgaaggag gcgactatga gacttatacc tatcttactg gccctgttgt tattggcggg
     8461 agggctgcat gccatcgatc cctggggtga gcctctcatc ctcactggca gcatgaccgt
     8521 catggcccag gtgcgcatca atggctttgc caccactccg ggagacgtcc tggccgcctt
     8581 tgtgagtgtg aacggagtgc cggaactgcg tggcaaacag gcaatcacgg tcaatagcaa
     8641 catctccggc tgcctgatcc agatttacac agacgccaat gacgaagaga tcaggtttat
     8701 ggtatgggac gaaagctccc agcaggtgta tgtctgcacc caggccctgg cctcgatggt
     8761 caacgggata gtgggaagct ggccctcaaa cctctatcag attaacgtga gcattcccct
     8821 caacacggat ccctggggcg cccccaccat tctgagctcc agtatgagcg tgatggccca
     8881 ggtgagcgtc aacggcagcg gagctgaatg gggagacatc ctggcggcct ttgtgagcat
     8941 cgacggcaca gaacacctgc ggggcaagac tcccatcctg atcaacgacg gagtgtccgg
     9001 atgcctcctg caggtctata ccgagtccag tggcgagccc atcaccttca aggtgtggga
     9061 tgccaatacc cagacgatct acgtgtgtcc ccagaccctg agctcggtgg tcaacggctc
     9121 ggtgggaagc tggccggcca acctgtacca gatcgatgca tcccagccag ccctcacgga
     9181 tccctgggga atcccaaccg tcctgagcgg cagcatgagc gtgatggcca gagtgttgat
     9241 caacaaccag gacgcgtcca atggagacgt cctgagcgct tttgtggatg tgcagggaac
     9301 cgaacaactg agaggcaagg cgaccgtggc catcaatgcc aacgagcccg gctgcctgat
     9361 ccagatcttc accgacagca atgatgagac catcagtttc aaggtgtggg actacagcgc
     9421 tcaggaaatc atcccggtaa acaacattct cccttcccag cttaatgcca tgattggcgg
     9481 ctggccggac aacctctatc agatcaacgc cgggcctgga gtccatgagc tcacaaaccc
     9541 catcttcgat cctccggatg gatactatgt ccagtctcag acggtgagca tctcttgctt
     9601 catccctggc gcccagattt attacacctt ggacggctcc gagcccacgc tcagcagccc
     9661 tctctacact ggtcccatcg ccgtcaacac cagcaccact atcaaagccc gggcctggct
     9721 cgcaggctgg tctcccagtg ccatttccac cgcccattac actatcaccg gccaggctga
     9781 ggcccccagc ttcaatcctg cccccgggac ctatacagcg ccccagaaca tcgtcatcag
     9841 cgctcccggg acgggcttga cgatccgcta caccctggac ggcagtgagc ccggcccggg
     9901 atcgccagcc tatgtgatgc cgatcaatct ggcccaggga gtccataccc tcaaggccag
     9961 ggtctggcgc cccggatgga atccaagccc taccaccacc ggtgtttaca ccattaccgg
    10021 cacggtcgcc atgccctttg tcaatccccc cggcggcacc tataccgttg cccagacgat
    10081 cactatctct tgctccaccc ccgggtccca gatccgctac acgacggatg gcagcgaacc
    10141 caatgacaat tccaccctct acacgggtcc ctttacagtc tcaagttccc tcaccctcaa
    10201 agcaaaggga tttaaggcag actgggcccc cagccagact gcctcggtag tttacaccat
    10261 caccggcacg gtggctatgc cgtcagccga tccacccgcg ggagtctata cccaacccgt
    10321 gacggtgagc ctcagttgca gcactcccgg agccgtgatc cgctatacca ccaacggcag
    10381 caatcccagc gtgggctcca ctccctatac cactcccatc ctgatcggca gcagcactcc
    10441 gctcaaagcc aaaggctttc tgagcggctg gaatgccagc cccaccctga ctgccaacta
    10501 cgccatcacc ggcaccgtgg ccacacccag tttcagccat ccctccggga cctacaaccg
    10561 gcacctcaca ttctcgatag cctgtgccac acccgccgcc cagatcagat acaccctgga
    10621 tgggaccgat cccgacagcg gttcggcgct ctacacggat tccatctata tcggtgccac
    10681 caccaccgtc aaggccagag ggtacaaaac cggctgggaa cccagcgaga tcgacagcgt
    10741 ggtctatcaa atctccctgg ccaatccgga cgcgccagat acacccttgc cacctggcat
    10801 caccagcgcc tggcccaatc ctttcacccg gcggatcacc atcgccgtca ggctcaaaga
    10861 agcttcccct gtcgccaaat tggggatcta caacctcaga ggcgagtgcg tgaggagttt
    10921 tgaggccacg ttcatcggtg aatacgagtt cgagtgggac ggctgtgatc agcgcggcag
    10981 gagaatgccc agcggaatct atctgctgag gctgaagagc ggccgcctgg cccaaacccg
    11041 caaaatcgtg ttcaaataga ggagggaccg gatatgaaac actcactgct caccctcgcc
    11101 ttcctctccg tctgctgttt gatcttagcc acagggcctc aggaacttgt gacggacaat
    11161 ccgacccgca ccgtcatctt tggcgacaac tttgattcct atcccaactt tgccaccact
    11221 ttcgcgccct ggactctggt ggaccaggac ctcagcaacg tgtttggctt tccgggcatg
    11281 accgttccca atatcggcaa tcccatggcc tacatgatct tcaacccctc ggccacccag
    11341 cctcccatga cagccgtgag cgctcacagc gggaccaaaa tggccgccag ttttgccgcc
    11401 accaaccctc ccaacagcga ttatctgatc acacccaggc tgcagcttgg caacttcagt
    11461 tcgctgagct tctgggccag gtctcacaac aacagctttg gtctggagcg cttccgggtg
    11521 ggcgtctgcg tgagcctcaa tcctgtcccg gcggcattta actggctgag cgccaactat
    11581 gtggaggctc ccgcctactg gacccaatac agctatgacc tcggcgcctg ggatggccag
    11641 cagatctata tcgccatccg ctgtgtatca gactattcca cggcctttta cgtggacgat
    11701 ttcaacgtga tgagcgacgt ggcccagcct cctcagctca taggcctgaa cgcgggctgg
    11761 aatctggtgt cattgcgagt gtctccctca gaccacagca tccccaccct cattcaaccc
    11821 attcagaact tcgtggaaca gatcaaaggc accgagggag tttacattcc cggcaatcct
    11881 ttcaacacgc tcacccagct cagcgacggc aaggcctatc tgatcaaaat gagcgcggcc
    11941 gccaattgga gcgtgagcgg attccagatt cccaccggca cacctttgcc tcttaccagt
    12001 ggttggaatc tggtggccta tctgcctcaa agctctatgg ctgtgaatat tgccacccag
    12061 agcatcgaga gctggctgct ggccgtcaaa ggcagcgatg gaatgtatta tcccggcaat
    12121 ccctacagca ctctcaccac catgtatccc ggcaagggct actggatgag gctcagtgag
    12181 gcgcgctcgc tggtgtatcc ttaggcgggg taaagaggat ctggaagtgg ccatggatcc
    12241 ggaaacgtct cggttccgct gggtgggtag tcttcagccg tgtttattga tataagccag
    12301 gataggaggc catgccacag gcgctgatcg cgc
//
LOCUS       DAOSFN010000070        12035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4481_length_12035_cov_8.625042, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000070 DAOSFN010000000
VERSION     DAOSFN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4481_length_12035_cov_8.625042"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(434..>3210)
                     /locus_tag="PLO35_05780"
     CDS             complement(434..>3210)
                     /locus_tag="PLO35_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH79212.1"
                     /translation="SHQQELLSPARRALTLFLLFLPILLSAANLSQRVDLAPASHDET
                     SLNSAGFGWQSAPGTLRLPVKTINLLLPPGAELQSWTAGLGSTVSLAGAAPQINPAFS
                     DGERSLNSPRRDNSSQRCIYLGLRNWGDLRFASFRVLPALWDEATERWLWSRDISLSV
                     EFTALEKAVAVVPPSFRQLDKRASFFANASDLDKWYSASGSKFYDILYVGTPASYAAI
                     SALEAFRNSQGFITSFANIATILAETAGSNPADKLRNYLISEYLAHPFTYLVLLGDYD
                     VVPVAMLTPEPDGYETVPSDFFYGDLSSIIDTDGDLLLGEYSSAEGNQDWGVDFTPEV
                     FVGRFSTNSATELEAIAARVVAFEQSEAAWKNNALLPAAFLNYQNEPETGFLQTDGAD
                     FMEYVKASVLSGMNCSTMYEQTGVVLSHPSDLALDYDLLKNLLSSQSYGLLNWSAHGS
                     STSSSRKVWINDDNANLLPDSWEMDWMNLVNRQSFNGLVNQDGMVIFMASCYNGMIDH
                     TGLSLAEHALVKKGVAVFGATRTGWYKIGWINPGWGGLSSYNYHVLENYVPHQMSVGA
                     AAAWTNLLHTQYYLFGDPVDSGGIIWPELQNVYTYLLYGDPVVGHTAAQTAPEGEILV
                     WEPMGNDAFKVINALHTSGRWNVVHTDKLIPDYDYIDRFDAVFALFGRGDTAYILNPA
                     SLEYNLLNSYLENGGKLYLEGMVGWDPMDTFWGKFGTHAPLDMLARIDAVGFNGSGID
                     YVWQYDQTDTYTDALLPFSASAIPIFHTVNVDYPQANIGIWNSDGQYRSIASSFSLGD
                     ILPGNPGIDELIQVICDTLDVGESAVVANDDPTTPAAMAGLKAWPNPFGGSVNISLEL
                     KYAAPVRMEVFNLRGQKLRTLNLPVAKGIRALSWDGRDASGTTCPTGIYILRATASGQ
                     PQTLKVLKIK"
     assembly_gap    3211..3310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3438..4451)
                     /locus_tag="PLO35_05785"
     CDS             complement(3438..4451)
                     /locus_tag="PLO35_05785"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOH79213.1"
                     /translation="MSFIKPANERQQEIYDMCLQYLYDHENPQVMIKNAPLYKEGYDA
                     FGLSDAEQRELRQMVLDRFNPSPQEIADLGKIFLSTHKFEFGLLAVQLIKKHRPRVDR
                     YVYEAVFEWLEQGIENIAHADLLATKITPVFLELGIASLDDFETWRTATSKWTRRVAA
                     LTMLYLRDKASPERLLEYIQPLLKDPEKVVQQGVGIFLRELWALHSDEVEEFLLNHRD
                     NTAPTVMQYATEHMQRDKKKRFGRAAMNRPPQSGNQPRKQNQPQNKRQNPNQRNNAQP
                     KPNSPHHAAGRVKPLRGRDMTPPLKKNNNKKNKLKPNVIVPEVPEMNDDNIPDWDSFD
                     DKF"
     gene            complement(4455..5237)
                     /locus_tag="PLO35_05790"
     CDS             complement(4455..5237)
                     /locus_tag="PLO35_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH79214.1"
                     /translation="MNSLCIDPNLVHIILVEPIYEGNVGAVARIMNNFGFHKLRVVGS
                     VPSKNDFYLAMHSEDILTEAEIFDDLAAAVQDLDRVIAFSRRVGKLKPIDLDPPAMAS
                     YALRSRHLRLGLVFGRETFGLTDAEADLCALRCHIPANPAFPSINLAQAVAIAVWELY
                     RLPLSERFETDSLPTTAAKKPAPAVSGKELDRLRDYMLEVMSGSGFFKDQESTNWDLF
                     LRKMLAQLNPGKTMAWRLRQMFNRWHVLATGKGKGYAHDTQE"
     gene            complement(5246..6067)
                     /locus_tag="PLO35_05795"
     CDS             complement(5246..6067)
                     /locus_tag="PLO35_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOH79215.1"
                     /translation="MIKEIGRLNLDDYKLMDAQVEKIFRNLDKDKIDLARKQITEMAN
                     TPNYFVREELGKRLANYDGPGAMEDVCGELLEDHIYGIRATALFYFYYNRRSDPAVII
                     RILEKTVESVPWESETILFELWKASPDLMKSTMPVWAQSPSAKKRAMSMHGMENIAIR
                     SPQFVLTFIENLIDDESEEVQDKISHVLTQVGRYRPLHTYVNIRRWLKDGDENRARVL
                     WNTMKKLASLIAQRNRKDQSPEFVSVTQRAIQDWRVDSAPIVSQMGNRLAQIIRN"
     gene            complement(6390..>8437)
                     /locus_tag="PLO35_05800"
     CDS             complement(6390..>8437)
                     /locus_tag="PLO35_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79216.1"
                     /translation="TQDSYARGNVSGGDRVGGVAGCTINGAVFRTYSTGYLNRGIVPG
                     APLPNWEGGWGGIVGLTSGSLPPGLGGGGGSGSVQNSYWLEQDNDDGYTNTDTASRTQ
                     TQLQDQSNVATNFPTWDYTNIWGWGGNDNYPILRATSSSVYYRSFATSDWDTASNWRT
                     DTTENGTYPTTPSVPPNAYNSLKITIQSGHTMTVGTDRTIDQTLVNSGGKILVNSGVT
                     LSLVDGTGTDLLIDGEVEHNGGLSLGLSTLTTVNGILKTNSGSTLNLEGDLVVNGAHT
                     ITSGANLSYATNSHKYFTGTTAQNAGTAFPSSVWDLTVNNPAGVSFPNNFTVNSVLYL
                     TSGSYSISGGLLPAVNGFVSPVVKYFDMKKLTVPTTGFSVDTNTSQSDPNYIERQWTV
                     TGHVNDASAFYRTKELTFYWTNDDDNNMNWGVRIPKVYVNDVGFNPKAGTFVIDGNNR
                     SIKFDYEFPQTESKATRNFKLGLGNDQTLPVQLSSFTAVVHQGNSVMLQWVSQTENNL
                     VGYRILRSTDEDAMNAILLDGLIEATNTSQAQSYVYFDREIYEPGTYWYWLQSLEMEG
                     SSQYHGPVSISYAPPQPETPELVLPAGFNTLYPNPFNPDLNIRFTVDQPGRATIEIYN
                     LRGQMLHRLMDSNLVKGSHKVIWDGRDAKGSLVSSGVYQLVLRSGSQRFSTRAVMMK"
     assembly_gap    8438..8537
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8538..9481)
                     /locus_tag="PLO35_05805"
     CDS             complement(<8538..9481)
                     /locus_tag="PLO35_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79217.1"
                     /translation="MNKTILNMILPVLMMTAIGLQAQTSVGNILELWNVRNNRSGSYT
                     QTGNIDLAVTDPAILQDWSNSQSYSVGEIREYTDGYAYYCIQASPAGTLPTNTAYWTK
                     LWEASKGWEPIGRTSATAFTGDYNGAGYQILNLYINRGASAVANNVYPSDGEDNVGLF
                     GYVENKLGSDTYIRSVTVVNPRVTGRRATGALVGRILLPITTPARSFTVYIERNAVYG
                     DGSSYVMGFGAVGGMVGANNSVAKQRVPVVRYNWANITVSATHPNNYTPNPNDRVGNT
                     NVYNPYNIKYGGLVGCNEIGITQDSYARGNVSGGDRVGG"
     gene            complement(9765..10790)
                     /locus_tag="PLO35_05810"
     CDS             complement(9765..10790)
                     /locus_tag="PLO35_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOH79218.1"
                     /translation="MDYTQYAVEKILELCRVPSPSGFTHLVRETLLKELQKLGFEPWT
                     TNKGSVLATLGGEGNHLVLAAHIDTLGAMVRAIKGNGRIRFTKIGGYPENNIENENVI
                     IHTRDGRTYTGTVYINGPAAHVYADSGSAKRDDNGMEIVLDEIVKSKDDTLKLGISAG
                     DFISLDSRTILTPSGFIKSRHLDDKASAGVQLALAAMVARGDLKLKRKLSILFTAYEE
                     VGHGASSGIPEDTVEMISVDMGAVGDDLGTDEYKVSICAKDSGGPYDYEVTSELINTA
                     KEQNLQYAVDIYPYYGSDVEGTLRAGYDLRHGLIGPGVFASHGYERTHTQAVENTLKL
                     LAAYVKA"
     gene            complement(11216..>12035)
                     /locus_tag="PLO35_05815"
     CDS             complement(11216..>12035)
                     /locus_tag="PLO35_05815"
                     /inference="COORDINATES: protein motif:HMM:NF027116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79219.1"
                     /translation="DSNGNCYVTGVFVGTATFGTLSINLSGGNNVFITKTGLPYPLIT
                     TNIPETVEFGNVYLDTSSPPFPVWIKNPGMADLVINTLSLQPTSSAFSIQPITLPFTI
                     AARDSVSIDLLFTPQGLGNATDILMISNNSQNQALYALQLSGTGIYAEPMPPANVTTH
                     INGYDYIITWDDVTQTVANTPITPDFYFIYFNGSPDPLGLFYFLGRSFNNQFTHYDVA
                     LGAEHMFYRVTAIKLTGRNVDMDWIDAQIRSGMTEGEVSAILKTLERPQPSAVK"
BASE COUNT         2583 a         3050 c         3334 g         2868 t
ORIGIN      
        1 cccgccggga ctcgtaagga atgatgccac gataatcagt tttcgcacaa gctctttagc
       61 cggtcgttgt gccggcttta gccggtatcc ctcaagaaag actataaagc gttataccaa
      121 tgccgtgaaa gcagataggg tcttatcacg caccgactaa agtcagtgct acgaccggct
      181 gaagccagtg atacggttat gtgcaactgt ctctgagata tgagaatatg agaaatccat
      241 catttgcatc aagtgcatcc atgagcgcct gcgtaaccca taacgcgtaa cgggtaacgc
      301 gtatcgcatc ccgcttattc acccgcggtg gagatgccgt gcgctctccc gggtttttcg
      361 ccggaggcgg gacggtatct tcacggcata tgatcaggca agcatggcgg ggatgatcaa
      421 cgcgctggat ggactactta atcttcaata ctttgagggt ctggggctgg ccgctggctg
      481 tggccctgag gatgtatatt ccagtgggac aggtggttcc ggatgcatcg cgcccatccc
      541 agctgagtgc tctgatgcct ttggcgacag gcagattaag ggtgcggagc ttctggccgc
      601 gcagattgaa gacttccatc cggaccggag cggcgtattt gagttccaga ctgatgttta
      661 ccgacccccc aaagggattg ggccatgctt tcaggcctgc catcgctgct ggtgtggtgg
      721 gatcgtcgtt ggccaccaca gcgctttcgc ccacatcgag ggtgtcgcag atcacctgga
      781 tcaattcatc aattccaggg tttccgggca ggatatctcc caggctgaag gatgaggcaa
      841 tgctgcgata ctggccgtcc gaattccaga tgccaatatt agcctgggga taatccacat
      901 tgacggtgtg gaagattggg atggccgacg cgctaaatgg aagcagggca tcggtatagg
      961 tatcggtctg atcgtattgc cagacgtaat cgatcccgga accgttaaaa cccaccgcgt
     1021 cgatgcgggc cagcatgtcc agaggcgcgt gagtgccaaa tttgccccag aatgtgtcca
     1081 tcgggtccca gcccaccatg ccttccagat agagttttcc gccgttttca aggtaggaat
     1141 tcaagaggtt gtattccagc gaagcgggat tgaggatata ggccgtgtct ccccttccga
     1201 agagcgcgaa gaccgcgtca aagcgatcga tatagtcata atccgggatc agtttgtcgg
     1261 tgtggaccac gttccagcgt ccggacgtgt gcagagcgtt gatcaccttg aaagcgtcgt
     1321 ttcccatagg ttcccagacg aggatttctc cctcaggagc tgtttgagcg gcagtgtgtc
     1381 ccaccaccgg atctccatag aggagatagg tatacacgtt ttgcagttcg ggccagatga
     1441 tgccacccga atccaccgga tcgccaaaga ggtaatactg ggtgtggagc agattggtcc
     1501 aggccgcggc cgctccaacc gacatctggt gaggcacata gttttccagc acgtgatagt
     1561 tgtagctgct gagaccaccc cagccgggat tgatccagcc gattttatac cagccggtgc
     1621 gggtagcgcc aaagacagcc acgccttttt tgacgagagc gtgctccgcc agggaaaggc
     1681 cagtgtgatc gatcatgccg ttgtaacagg aagccatgaa gatcaccatg ccgtcctgat
     1741 taactaaacc gttgaagctc tgacggttca cgagattcat ccagtccatt tcccagctgt
     1801 cgggtagcag attggcgttg tcgtcgttga tccagacctt gcgggatgag gaggtggaag
     1861 agccatgggc gctccagttc aacaggccgt aactttggga actgagcagg tttttaagga
     1921 ggtcgtaatc gagggccaga tcgctgggat gggaaagcac cactccggtc tgctcataca
     1981 tggtgctgca gttcatgcca gatagaacgg aggctttgac gtattccata aagtcggccc
     2041 cgtcggtctg cagaaatccg gtttccggct cattttgata gtttagaaaa gcggccggca
     2101 ggagggcatt gtttttccag gcggcttcag actgctcaaa agcgaccacg cgggccgcga
     2161 tggcttccag ttcggtggcg gaattggtgg agaagcgtcc cacaaagact tccggggtaa
     2221 aatccactcc ccagtcctga ttgccttcgg cgctggagta ctcacccagg agcaaatcgc
     2281 cgtccgtgtc gataatgctg ctcaggtcgc catagaagaa atccgagggc accgtctcat
     2341 agccatccgg ttcgggagtc agcatggcca ccggtaccac gtcataatct cccagcagca
     2401 cgagataagt gaagggatgg gcaagatatt ctgagataag atagttacgc agcttatccg
     2461 ccggattgga gccagccgtt tcggccagga tggtggcgat attggcaaag gaggtgatga
     2521 agccctgaga gttgcggaag gcctccagag ctgagatggc ggcataggaa gctggcgtgc
     2581 ccacatagag gatgtcataa aacttgctgc cgctggctga gtaccatttg tcgagatcgc
     2641 tggcattggc aaagaaggaa gcgcgcttgt caagctgccg gaagctggga gggaccactg
     2701 ccacggcctt ttctaaagct gtaaactcca cgctcaggct gatgtctctg ctccagagcc
     2761 agcgctccgt ggcctcatcc caaagggcgg gaagcactct aaaagaggca aaccgcaggt
     2821 ctccccagtt tctgaggccc agatagatgc agcgctgcga gctattgtct ctgcgagggg
     2881 aattcaagct ccgttcgcca tcgctgaagg cgggattgat ctgaggggcg gcacctgcga
     2941 ggctgacggt agagcccaga ccggcagtcc aggattgcag ttcagcgcca gggggtaaaa
     3001 gcaggttgat tgtcttgact ggcagtcgta aagtcccggg agcgctttgc cagccaaagc
     3061 cggcggagtt cagagaggtc tcgtcatggc tggcaggggc caaatccact ctctggctta
     3121 agttggcagc agacagcagg atagggagga aaaggagaaa taaagtgaga gctctgcgag
     3181 ctggtgagag cagttcctgc tggtgagagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn ggtctaccgg aaacgaggat gtttccggct cagttagtga aagcaatttc
     3361 aattcccaac aaacaagcat cccgatcatc ccttttttcc ccgtttcttc gttctataaa
     3421 ttctcaattc tcaatttcta aaacttgtcg tcgaagctgt cccaatccgg gatgttgtca
     3481 tcattcattt caggcacttc cggcacgata acgttgggct tgagcttgtt ctttttgttg
     3541 ttgttctttt tgagcggagg ggtcatatcc ctgccacgca gaggtttgac tcttcccgcg
     3601 gcgtgatggg gagagtttgg tttgggttgc gcgttgttgc gttgattggg attctgtcgt
     3661 ttgttttggg gctggttctg ctttctgggc tggtttccag actggggcgg gcgattcatg
     3721 gcggccctgc caaagcgctt tttcttgtcc cgctgcatat gctccgttgc gtattgcatc
     3781 acggtgggag cggtgttgtc ccggtggttg agcaaaaact cctccacttc gtcagagtgc
     3841 agggcccaga gctctctcaa aaagattccc actccctgct gcaccacctt ttccgggtcc
     3901 ttcaaaagag gctggatgta ttccagcagg cgttctggag aggctttgtc tctcagataa
     3961 agcatggtga gcgcggccac tctgcgggtc cacttgctgg tggccgtcct ccaggtctca
     4021 aaatcgtcca gcgaggcgat ccccagttcc aaaaagacag gcgtgatctt ggtagccagt
     4081 agatcggcgt gggcaatgtt ttcgatgccc tgttccagcc attcaaacac cgcctcatag
     4141 acatagcggt ccacgcgggg acggtgtttc ttgatgagtt gcacggccag gaggccaaac
     4201 tcaaacttgt gggtggagag gaagatcttg cccagatcgg caatttcctg aggactggga
     4261 ttgaagcggt ccagcaccat ctgtctcagc tcgcgctgtt cggcgtctga gaggccaaag
     4321 gcgtcgtaac cttccttata caggggggcg tttttgatca tgacctgggg gttttcgtgg
     4381 tcgtaaagat attgcaggca catatcatat atttcctgct gcctttcgtt ggcgggcttg
     4441 ataaagctca tggcttactc ctgagtatcg tgggcatagc ctttcccctt tccggttgcc
     4501 agcacgtgcc agcggttgaa catctgtcgc agcctccagg ccatggtctt gcctggattt
     4561 agctgggcca gcattttgcg cagaaagagg tcccaattgg tgctctcctg gtccttgaaa
     4621 aagccgctgc cgctcatcac ctccagcata tagtcacgca ggcggtccag ctctttgcct
     4681 gaaacggcag gggccggttt tttggcagca gtggttggca gggagtcagt ttcaaagcgt
     4741 tccgaaaggg gaaggcggta tagctcccag acggcaatag ccaccgcctg ggcaagattg
     4801 atggatggaa atgccggatt ggcggggata tggcatcgga gggcgcaaag atcagcctcg
     4861 gcatcggtga ggccaaaggt ctcgcgtcca aacaccagcc ccagcctgag gtgccggctc
     4921 ctgagcgcgt aggatgccat ggcgggtgga tcgaggtcga tgggcttaag cttgcccacc
     4981 cgtctggaaa aagcgatcac cctgtcgagg tcttgcacgg cagcggccag atcatcaaag
     5041 atctcagctt cggtgaggat atcctcagag tgcatcgcca gataaaagtc gttcttgctg
     5101 ggcaccgatc ccaccactct gagcttatga aagccaaagt tgttcatgat tctggccacg
     5161 gcgcccacgt taccctcata gatgggctcc accagaatga tgtggacaag attgggatcg
     5221 atgcagagtg aattcaatcc agagctcagt ttcggatgat ctgggccagg cggttgccca
     5281 tctgagatac gatgggagcg ctgtccactc tccaatcctg gatagcgcgc tgggtcacgg
     5341 agacaaactc agggctctgg tccttgcggt tgcgctgagc tatcaggctg gcgagcttct
     5401 tcatcgtatt ccagagcacc cgggcccggt tttcatcgcc gtctttcagc cagcgacgga
     5461 tgtttacata ggtgtgcaaa ggacggtaac gccctacctg agtgagcaca tgggatattt
     5521 tatcctgcac ttcctcactc tcgtcatcaa tcaggttttc gataaaggtg agcacaaatt
     5581 ggggactgcg gatggcgatg ttttccatgc cgtgcatgct catggcgcgc ttcttggcgc
     5641 tgggagattg cgcccagacc ggcatggtgc ttttcatcag gtccggactg gccttccaga
     5701 gctcgaagag gatggtttcg ctctcccagg gcacactttc caccgtcttt tccaaaatcc
     5761 tgatgatcac ggccggatcg ctgcgccggt tgtagtaaaa gtaaaagagc gcggtggcgc
     5821 gaatgccgta aatgtggtcc tcgaggagct cgccgcagac gtcttccatg gcgccggggc
     5881 catcatagtt tgccagcctt ttgcccagtt cttccctcac aaagtagttg ggggtgtttg
     5941 ccatttccgt gatctgtttg cgggcaaggt cgattttatc tttatccaga ttgcggaaga
     6001 tcttttccac ctgggcgtcc attagtttgt aatcgtccag atttagacga ccaatttctt
     6061 tgatcatgtg cgtatgctcc agctatgaat gtctcgcgcg ccgcaaaaca cattgttaca
     6121 tataaatcag ggaatcatct ctcccgcggc gcttgatgta aggcattttg ttttatgatg
     6181 ttatcatcgc tctttggtgg ccgaggcggc cgtttttgag atagcgatct gagaacccat
     6241 gttacctggg ggaaaagata atcgaggccg caaatctgtc aagcgctttc tgccagccag
     6301 gcaaagaaca ggctctcaga cctcagggtg tccataatca aaaaaggccg aaccctgcgg
     6361 gaacggcctt tctattcagg agttatagtt tatttcatca tcacggcacg ggtgctgaag
     6421 cgttggctgc cgctccgcaa caccaattga tacaccccgc tggaaacgag gctgcccttg
     6481 gcgtcgcgtc catcccatat cactttgtga ctgcccttga ccaagttcga gtccatcagc
     6541 cggtgcagca tttgaccgcg cagattgtag atttcgatag tcgcccggcc aggttgatcc
     6601 actgtgaagc ggatattgag atcgggattg aagggattgg gatagagagt gttaaatcca
     6661 gccggcaaca ccagttcagg cgtctcaggt tgcggaggag cgtagctgat gctgacaggc
     6721 ccgtgatact gagaagagcc ttccatctcg aggctttgca gccaatacca gtaggtgccg
     6781 ggctcataga tctcacggtc gaaatacacg tagctctgcg cctgggatgt attggtggct
     6841 tcgataaggc catccagcag gatcgcgttc atggcatctt cgtctgtgga cctcaggatc
     6901 cggtagccca ccagattgtt ttctgtctgg ctcacccatt gcagcatcac gctattgccc
     6961 tgatggacca cggccgtgaa gctggaaagc tgcaccggca gggtctgatc gttacccagg
     7021 cccagcttga agttgcgggt ggctttggac tcggtttgcg ggaactcata gtcgaacttg
     7081 atgctgcggt tgttgccgtc gatcacaaaa gtcccggctt tgggattgaa gccgacgtcg
     7141 ttcacataca ctttgggaat cctcactccc cagttcatgt tgttgtcatc gtcattggtc
     7201 cagtagaacg tgagttcttt ggtgcgatag aaagcgctgg cgtcgtttac atgaccggta
     7261 accgtccact ggcgctcgat gtagttggga tcgctctggc tggtgttggt atccactgag
     7321 aagcctgtgg tgggtaccgt gagctttttc atatcaaaat acttcacaac cggagacaca
     7381 aacccgttca cggcaggcag caagccaccg gaaatgctgt aggacccgct ggtgagatag
     7441 agcacgctat tgacggtgaa attgttgggg aaactcacac ccgcgggatt gttgaccgtg
     7501 aggtcccaaa cactggatgg gaaagccgta ccagcgtttt gggcagttgt tccggtgaaa
     7561 tatttatggg aattggtagc atagctgaga ttagcaccgc ttgtaatcgt atgagcccca
     7621 ttgaccacca ggtctccttc cagattcagg gtagagccgc tgttagtctt cagaatcccg
     7681 ttcacagtgg tcagggtgct caatcccagg ctcagtccac cgttatgttc cacttcacca
     7741 tcgatcagca gatcggtgcc ggtcccatct accaggctga gagttacgcc actgtttacc
     7801 aatatcttgc cgccgctgtt cacaagcgtc tgatctatgg tgcgatcggt gccgacagtc
     7861 atggtgtgtc cgctctggat agtgatcttg agagaattgt aagcattggg aggcacactt
     7921 ggagtggtgg gataggtccc gttttcggtg gtgtcggtgc gccagttgga agctgtatcc
     7981 cagtcactgg tagcaaaaga cctgtaatac acgcttgagg atgtggctct caggatagga
     8041 taattgtcgt ttccacccca gccccagata ttggtatagt cccaggtggg aaagtttgtg
     8101 gctacattgg actggtcctg aagctgggtc tgagtacgtg aagcagtatc agtattagta
     8161 taaccgtcat cattgtcctg ctccagccaa tagctgtttt gcacgcttcc tgatccacct
     8221 cctccaccca aaccaggagg aagtgatccg ctggtgaggc caacgatacc accccagcca
     8281 ccttcccaat ttggaagagg agcgcctggc actattccac ggttcagata accggtggaa
     8341 taggtgcgaa agacagcccc gttgatggta cacccagcca ccccgcccac acggtctcct
     8401 cccgagacgt tgccacgggc atagctgtcc tgggtgannn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnccg cccacacggt ctcctcccga gacgttgcca cgggcatagc
     8581 tgtcctgggt gatgccgatt tcgttgcaac ccaccaaacc accgtatttg atgttgtagg
     8641 ggttgtaaac attggtattc cccactctgt cgttggggtt gggggtatag ttattggggt
     8701 gagtagcgct cacagtgata ttggcccaat tgtatctcac caccgggacg cgctgtttgg
     8761 ccacgctgtt gttggccccc accatcccac ctaccgcacc aaagcccatc acataggaag
     8821 aaccgtcgcc ataaacggcg ttgcgctcga tatagacggt gaaagatctc gcgggagtag
     8881 tgataggcag caaaatcctg cccaccaggg ctccggtagc tctgcggcca gtgacgcgcg
     8941 ggtttaccac ggtcacactc cggatatagg tatccgaccc cagtttgttt tccacatagc
     9001 caaagaggcc cacattgtct tctccatcag agggataaac gttgttggcc acggctgagg
     9061 ccccgcggtt gatgtaaagg ttcaggattt ggtatcccgc cccgttgtag tctcccgtaa
     9121 aggcagtggc agaggtcctg ccgatgggct cccagccttt ggaggcttcc cacaacttgg
     9181 tccaataagc cgtgttggta ggcaaggtcc ctgctggaga tgcctgaatg cagtaataag
     9241 cataaccatc cgtgtattct ctgatctcgc caaccgaata cgattgcgag tttgaccagt
     9301 cctgaagaat ggctggatct gtaaccgcca ggtctatatt gcctgtctga gtgtatgatc
     9361 cactgcggtt gttgcgtacg ttccagagtt ccaggatgtt tcctacactg gtttgggctt
     9421 gcagcccgat cgccgtcatc atcaatacgg gcagtatcat attcaatatt gttttgttca
     9481 taatataact cctgcggggc cgagatcggt atttgacttt ccacgataat ctcaacacca
     9541 cgcttagaca atatattaaa atatcacagt acgcgcaaga aaaaaaacgc tgaagccctc
     9601 tggacagcga tatatgtgct acaattaatg agaaggattg gcaggattca tcgcgcccgg
     9661 agctcaaagc gccgccacac caaacacatc cctctctgta tggtttggcg cttgcggctt
     9721 caatcagtgc ggaagaaaaa gtgctggttg aattcccgcc gtttctaagc tttcacatag
     9781 gcggccagca gtttgagggt gttttccacc gcctgggtgt gagtgcgttc ataaccgtga
     9841 gaggcaaaga caccgggccc gatcagacca tgacggaggt cgtaacccgc tctcagagtg
     9901 ccttccacat ccgagccgta gtagggatag atatccaccg cgtattgcag gttctgctct
     9961 ttggcggtat tgatcagctc actggtcacc tcatagtcat agggcccacc ggaatccttg
    10021 gcgcagatgg agaccttgta ttcatcggta cccaggtcgt ctcccacagc tcccatatcc
    10081 acgctgatca tctctacagt gtcttccgga atgccggagg aagcgccatg acccacttcc
    10141 tcataagcgg taaagaggat gctgagcttg cgcttgagct tcagatcacc cctggcaacc
    10201 atagccgcca aagccaattg cactcccgcg ctggctttgt catccagatg acggctcttg
    10261 atgaacccag aaggagtgag gatggtgcga ctgtccagac tgataaaatc accggcgctg
    10321 atgcccagct tgagggtgtc gtctttgctt tttacgatct catccagcac gatttccatg
    10381 ccgttgtcgt cccgtttggc gctgccggaa tcagcataga cgtgcgcggc cggaccatta
    10441 atatagacgg tgccggtata ggtcctgccg tcacgggtat gaatgatgac gttttcgttt
    10501 tcgatattgt tttccggata gccaccgatc ttggtgaaac ggatgcgtcc gttgcctttg
    10561 atggccctga ccatggcacc cagggtgtcg atgtgggcgg ccaggacgag gtgattgcct
    10621 tcccctccca gagtggccag caccgatcct ttgttggtgg tccagggctc aaaacccagc
    10681 ttttgcaatt cttttagcag cgtctccctc accagatggg tgaatcctga tgggctggga
    10741 acgcggcaca gctccaaaat cttttcaacg gcatattgag tgtagtccat gatagctccc
    10801 gctaatgttt tgatgtggcc aggctggcga agagcggaat tatggcaagg tttttttgtg
    10861 ggtgccacct gccggtgaga gccttgcggg aggcataacg cagcttcggc aaaagattta
    10921 tgatcacgtc ctccacattg gcaaaactga cgaaggcgcg ctttttgctg cagtggagga
    10981 ggggttccgg cccttttttc gggctggctt gccaaggtat ctcttccaga agtgaatcct
    11041 tggagctcag catctgaatc cggccaggga aacatgagaa tccacccaga tttccgtttt
    11101 cctgcctggt actctctgca tcctattacg gactcattac ggactaagtc cgtaatgagt
    11161 ccgtaatgac tccgtaatgc taatcagaga ggaggtggaa acaaatagcc tccagctact
    11221 tgaccgccga aggttgtgga cgttccagtg tcttcaggat tgcggatacc tccccctccg
    11281 tcattcccga tctgatctga gcgtcaatcc agtccatgtc cacattcctg ccggtaagtt
    11341 tgatagctgt cacccggtag aacatatgtt cggcgcccag ggcaacgtca taatgcgtga
    11401 actggttatt aaatgaccgc cccagaaagt agaataagcc cagaggatca ggcgaaccat
    11461 tgaaatagat gaagtagaaa tcgggcgtga tgggggtatt tgctacggtc tgggttacat
    11521 catcccatgt gatgatgtaa tcgtaaccgt ttatatgagt ggtcacattg gcgggtggca
    11581 tgggttcggc ataaattccg gtgccactta gctggagggc atacagggct tgattttggg
    11641 agttgttgga gatcatcagg atgtccgtcg cgttccccag cccttgaggt gtgaacagca
    11701 gatcgatgct tacgctgtct cttgccgcaa tagtgaaggg caaggtgatc ggctggatcg
    11761 aaaaggctga gcttgtcggt tgcaaactga gtgtattgat caccagatca gccatgccag
    11821 ggttcttgat ccacacggga aaaggcgggg aacttgtatc cagataaacg ttcccgaatt
    11881 ccactgtttc tgggatgttg gtggtgataa gcggataagg aagtccggtt ttggtgatga
    11941 agacattatt gccaccgctg aggttgatcg ataaagtgcc aaaagttgcg gtgccgacga
    12001 atactccggt cacatagcag ttgccattgg agtcg
//
LOCUS       DAOSFN010000071        11888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4537_length_11888_cov_8.690188, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000071 DAOSFN010000000
VERSION     DAOSFN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4537_length_11888_cov_8.690188"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLO35_05820"
     CDS             complement(<1..417)
                     /locus_tag="PLO35_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79220.1"
                     /translation="MSKTLSLVTLMLLALSLLSAGDYIIGSDTSTESYVPVYGYNHYG
                     WSKFFYTAAEMQAAGFTTPQQITRLAFFVDNSPVDYVMDDQRVYMTHFYDSAYGSTAA
                     GYPNPSTYTYVFNGSVTWSGPGWNEIVLTTPFNYDPS"
     gene            complement(429..>1361)
                     /locus_tag="PLO35_05825"
     CDS             complement(429..>1361)
                     /locus_tag="PLO35_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79221.1"
                     /translation="AQYQGILAYASTGTVSAKMYYFATALSDFLDLGTQSSPKNLWFA
                     SDGLASNQHAHPNSSSIRRMMSGYFRTHYVASGLGGGSNGLGGPDSFSYEHGTIKALP
                     GTQVGMNGEEYPVYANSPDCIFPNDAAGDPYYDEVPYPAIGAIYNYAFEDGPFGGQAY
                     LYHGVCATQVDTPSYRTMYFSFDLSQLTSAAHRLEWMSDLMDWWDVGPVSNEDPQIPA
                     LRTGIDSVFPNPFNPSTTIRYSLDSAQALDLAIYNLKGQRVKTLARDLKNAGTHSAVW
                     NGLDDSGRPVSSGVYLLRLNTKARQQTQKITIIK"
     assembly_gap    1362..1461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1462..3563)
                     /locus_tag="PLO35_05830"
     CDS             complement(<1462..3563)
                     /locus_tag="PLO35_05830"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOH79222.1"
                     /translation="MKHILMIALLLTASSLLLGQWHIEEGFEGITTLPAGWTIFDDGD
                     GMTWRNLSHANAHTGSRAAFVDNYLPNENADWLVTPQITVSPGDSLFFFTRSWVSTED
                     LKVFVSTAAPNPSALNQLLVHLTNIGTPYQQVSLPLNQWAGQSIYLGFYWECNTYGIL
                     VDDIKIGQPLNVTPELDLPQSISFFESDTYTLDLIPHIVCTDLAHTSIAVAPNPNLNV
                     SVSGLDLVLSSNGFTGSTQLYVTLTDLLNNLSAADSMEVLVLSDPAVDLYVSQVLAPR
                     VYEFVGMPFTPQVRVINLGTSSFNDQIEISLSIYDNNGSPVHSDIAIQNVDLGPQQST
                     DLLFPLDFTPLSEGTFSYNLTINTPDGNPGNNQTQASFSILNRITLGGPDAFGYRFLD
                     SNNPLGPEYEWTDISQTGTSTISYGVNGWGGDDNFSEPIPLGFNFPFYGSSYDQAYVD
                     INGEILLAPNTWYNAYPGLNWDNDGNMFNYMYPIPGYTQMPGLIAVYWDDLHADEGTG
                     NIYYQTFGEAPDRYTVFQWHDLRFHAGTGGTPQLKFQAILFEDGNIKLQYHTVATGQT
                     GASVPHDNGFSATVAIQNQAANAGLPYLREIVQNSQYQGIEPAGNVLHPQLAVLFYAG
                     TDQQAPIITHRPMGNTLNQNLELVARIIDMSALPTTHLHYNTGAGWQVLSPHSIQQND
                     YYFMLTDLPLGSTVQYYF"
     gene            complement(3575..4939)
                     /locus_tag="PLO35_05835"
     CDS             complement(3575..4939)
                     /locus_tag="PLO35_05835"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOH79223.1"
                     /translation="MIKNLQIISKHSQVKELGNALEFDMLKELIRSAATCQQLATAFS
                     VSKQKVHYNLTKMMEEGLIEVVEEPGNGKEVYYRATAKNYVLDFSLGEHLGGGLINSR
                     GVINNILEGEYNLNLSAIAAKLLKHSLKLRSRQKLLIVTGKYNLPLVEKILLEAGRMN
                     IRCTTIYQDSQLLKAKYEEYSLTAFHADYDHFNRLLKAHDAYLNLNGEARHIQLSDPE
                     KIQIRAMAYAASHGILKDRKIRVAMMPGLLNDTLSENSIESELQFWKALDVDYEKLSQ
                     ETMSICSNFASAGEIKLSSYEASLTFQTERVLAESGSFGESPCQSPVINYPGGEVLIL
                     PKSASAHGRIRGDVAYAFGEKIIQPSLEIRNGEISSFSAAVNQDLLARAIAEGGSDGR
                     KVALICMGTNPNVSLSNIDMSFSQKSRGLVTVWWGDNRSIGGQVTGGCEWFVQIEKPS
                     LKSL"
     gene            complement(5153..5578)
                     /locus_tag="PLO35_05840"
     CDS             complement(5153..5578)
                     /locus_tag="PLO35_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOH79224.1"
                     /translation="MSDKRLPANDRRPGNSLGRVFLIIGGSLALGLALLGVILPLLPT
                     TPLLLLAAVCYGRSSARLYHWMHHNALFGEYLRRYQAREGIPVRVKLFTLGLLWAGIL
                     SSALLFIPGRLWPVRIMLLLVAVGVSIHVLTIKTFRGKN"
     gene            complement(5591..7063)
                     /locus_tag="PLO35_05845"
     CDS             complement(5591..7063)
                     /locus_tag="PLO35_05845"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOH79225.1"
                     /translation="MDEKKKKKKLTTVAGTPVVDNQNIMTAGPRGPLLMQDVWLVEKL
                     AHFDREVIPERRMHAKGSGAFGHFTVTHDISQYTKAKIFSEIGKQTEVFARFSTVAGE
                     RGAADAERDIRGFAVKFYTEEGNWDLVGNNTPVFFIRDPLKFPDLNHAVKRDPRTNMR
                     SAKNNWDFWSSLPEALHQVTITMSDRGIPYSYRHMNGYGSHTFAMYNKDNTRVWVKFH
                     LKTLQGIKNLTDAEAEAIIATDRESHQRDLYESIEKGDFPRWEMSIQVMSEEEATQVP
                     FNPFDITKVWSKKHYPLIKVGILELNRNPANYFADVEQAAFTPANIVPGLGFSPDRLL
                     QARLFSYGDAQRYRLGINHHQIPVNRPKAEAHSYHRDGQMRVDGNYGSTLGYEPNSYG
                     EWQDQPDFKEPPLDLAGAADHWNHRDDADYYSQPRALFQLMSPEQQQVLFENTARNMG
                     DAPLEIKLRHIRNCHKADPKYGEGVAKACSIPMEEVLKGI"
     gene            complement(7056..7550)
                     /locus_tag="PLO35_05850"
     CDS             complement(7056..7550)
                     /locus_tag="PLO35_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOH79226.1"
                     /translation="MINKQLVSQRIERFKMLMIEKGYKLTQQRLEIYKALAAREDHPS
                     AEEIHKRLRKRLPTLSLDTVYRTLWTFRDLGIIATLNPHHGVARFDANHSHHHHFVCE
                     RCGAVQDYDNHEFNAIPLPADLGNMGEAKSLHVEIRGLCRKCLADKDEINSPTDQDKE
                     QGNG"
     gene            complement(7673..>9842)
                     /locus_tag="PLO35_05855"
     CDS             complement(7673..>9842)
                     /locus_tag="PLO35_05855"
                     /inference="COORDINATES: protein motif:HMM:NF021831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ser-Thr-rich GPI-anchored membrane family
                     protein"
                     /protein_id="HOH79227.1"
                     /translation="AQNVPLVLTAPNGGEQWQAGSVYDITWEQTNLAGAVSLHLISPL
                     SSQPPLAIAMNIPVQAQLFHWQIPASIMPGDFYKVMISMASNPSNSFSDISDAPFTIL
                     GGDPPPPPVQITVQNPNGGEIWPLGSTQTINWTSQGLEGDVVITLVQANGVADYQIAA
                     QVPIMPGNFTWTVPDDIMPGNGYRVHVMWLSDLTVYFGDVSDLPFSIVGSDPPPPQNL
                     QLISPNGAEQWETGTTRPIHWVCSSTAAMPVTLKLYNGLNTNTPPILIAQNLPPFPGF
                     FHWQIPATLAPSEQYYMQVSSLSPDGTIQMDFSDGPFSILNGTPPPPPQMLQILSPNG
                     GETWVAGTMHPITWVQGFNAILPVTLTLHDGMDPAVAPIPIAVNIPSFPSLFHWMIPS
                     GLPLSNQYLVRISTPMADGTLVEDWSDSSFSIVNGNPPPPQTLRVISPNGGEHWMAGT
                     MHPIRWHAPQQQGPVEIALMRGDDPQPALVIAPGLPIPGMFRWHIPMNLEAASDYRIR
                     IRSLNANLADLSDAPFTILAHQAPPPSNVTLTSPNGGELWQAGNSYTITWNAPDLNVP
                     MQLWLLREGQRERYRHIISSSLPNTGSFAWTIPPGVPSGDNYKVMVRAIHAPGQMDIS
                     DAVFSIVTNPAKLKATTNKGSDAVTISLDSESGIQAQVQVYNIRGQKVRDLGTLALGS
                     TKSVIWDTRDDRGQKTSPGLYLIRVKVDREYLSQRVLLTK"
     assembly_gap    9843..9942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10205..11833
                     /gene="groL"
                     /locus_tag="PLO35_05860"
     CDS             10205..11833
                     /gene="groL"
                     /locus_tag="PLO35_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198340.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOH79228.1"
                     /translation="MAKQMLYAHDARTSLKKGVDQLADAVKVTLGPRGRNVVLDKKFG
                     SPTITNDGVTIAKEIELENEFENMGAQLCKEVAEKTHDVAGDGTTTATILAQAIIEEG
                     LKHVTAGVNPMYLKRGLEKASKVIVDQIHKFSKEIKSSEEVAQIATISANNDPEIGKL
                     IAEAMDSVGREGIINIEEAKSIDTGLEKVEGMQFDRGYLSPYFVTNPEKMIAELEDPF
                     ILIYDKKISVMKDLLPILQETAQTGKPLLIISEDIEGEALATLVVNKLRGILNVVAVK
                     APGFGDRRKAMLEDISILTGATLISEDMGRKLDSCTLTDLGRAKKVIVEKENTTIREG
                     AGSQDAIDGRVKQIKAQVEETTSDYDKEKLQERLAKLSSGVAVIRIGAATETEMKEKK
                     ARVDDALHATRAAVEEGIVPGGGVTLIQAAKALKEMKDLSHEEQMAVEILVKALAKPA
                     YQIATNAGEEGAVIVEKLKEYTDVHMGYNAANGKFEDLFKAGIIDPAKVVRSAVQNAT
                     SIAALLLTTECIVTDIKEPEAPAPMPNPGMGGMY"
BASE COUNT         2640 a         3010 c         3424 g         2614 t
ORIGIN      
        1 ggagggatcg taattgaagg gagtggtaag aactatttca ttccaccccg ggccactcca
       61 ggtgacagag ccattgaata cataagtata agtggatgga ttagggtagc ctgccgcggt
      121 tgagccgtag gcagaatcat agaaatgggt catataaacc cgctggtcgt ccatgacata
      181 atcaaccggg ctgttgtcaa caaagaaagc taaacgggtg atctgttgtg gggtggtaaa
      241 tccagcagct tgcatctcag ccgctgtgta gaagaactta ctccagccgt aatggttgta
      301 tccatacacc ggcacataac tttcagtact ggtatcgctg ccaataatgt aatccccggc
      361 gctcagcaag ctgagggcca gtagcatcag ggtgacgagt gaaagtgttt tggacatctt
      421 gactcccttt atttgatgat agtgatcttt tgggtttgtt gtctggcctt ggtattgagc
      481 ctcagcagat atactccgct ggagacggga cggccgctgt cgtccaggcc gttccaaacc
      541 gcgctgtgag tcccggcgtt tttgagatct ctggccaggg tcttcaccct ctggcctttg
      601 aggttataga tggccaggtc cagggcctga gcgctgtcca ggctgtagcg gatggtggtg
      661 ctggggttga agggatttgg gaagacggaa tcgatccccg tccgcagagc cggtatttga
      721 gggtcttcat tactcacggg accgacatcc caccaatcca tcaggtcgct catccattcc
      781 aagcgatggg ccgcgctggt gagctgcgaa aggtcgaagc tgaaatacat ggtgcggtag
      841 gaaggagtat ccacctgggt ggcgcaaact ccatgataga gataggcctg gccgccaaag
      901 gggccgtctt caaaggcgta gttatagata gcgccgatgg caggataggg cacttcgtca
      961 tagtaaggat ctccggctgc gtcgtttgga aagatgcaat cgggtgaatt ggcatagacg
     1021 gggtattcct ctccgttcat ccccacctgg gtgccaggca gggctttgat ggtgccatgc
     1081 tcatagctaa aactgtctgg acctcccagg ccattagacc cccctcccag tccagaagcg
     1141 acataatgag tgcggaagta tccactcatc atccttctga tgctgctgga attggggtgg
     1201 gcgtgttgat tgctggccaa tccgtctgag gcgaaccaga ggtttttggg tgagctttgc
     1261 gtgccgagat caagaaaatc tgaaagggcg gtggcgaagt aatacatctt ggcgcttacc
     1321 gttccagtgc tggcataggc caggatgccc tgatactgag cnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn ntcaaagtaa tattggactg tggagcccag aggcaaatcc
     1501 gtcagcatga aataatagtc gttttgctgg atggaatgag ggctgaggac ctgccatccg
     1561 gcaccggtgt tgtagtgcag atgggtggtg gggagggcgg acatatcgat gatcctggcc
     1621 accagctcca ggttctggtt gagagtgttg cccatggggc ggtgtgtgat gatgggggct
     1681 tgctgatcgg tgcccgcgta gaacagcacc gccagctggg gatgcaagac gtttccggct
     1741 ggctcaatgc cttgatactg gctgttttgc acaatctctc tcagataggg aagcccggca
     1801 ttggcagctt gattctggat ggcgacggtg gcggaaaaac cgttgtcgtg agggacggaa
     1861 gctccggtct gacctgtggc cacggtgtga tactgcagct tgatgttgcc atcttcaaag
     1921 aggatggcct gaaacttgag ctggggcgtt ccacccgtcc cggcatggaa tctcagatcg
     1981 tgccactgga acacggtata acggtctggg gcttcgccaa aggtctgata atagatgttt
     2041 cccgtgccct catcggcatg caggtcgtcc caatagaccg cgatcagacc aggcatctga
     2101 gtgtaacccg ggatgggata catatagttg aacatattgc cgtcgttgtc ccagttcaag
     2161 cctggatagg cattgtacca ggtattgggt gccagcagga tttctccgtt gatatccaca
     2221 taggcctggt cgtaggagct gccgtagaac ggaaagttga agcccagagg gatgggctcg
     2281 ctaaagttat cgtctccgcc ccagccgttt acgccatagc tgatggtgga agtgccggtc
     2341 tggctgatgt ccgtccattc atattcagga cccaggggat tgttgctgtc caaaaagcgg
     2401 tagccaaacg cgtccgggcc gcccagggtg atccggttga gaatactgaa gctggcttgc
     2461 gtctggttgt tgccagggtt gccatcggga gtgtttatgg tgagattata gctgaacgtc
     2521 ccttcactca ggggtgtaaa gtccaggggg aaaagcagat cagtgctctg ctggggtccc
     2581 aggtccacat tctggatggc gatgtcgctg tgaaccgggc tgccattgtt gtcatagatg
     2641 ctgaggctga tctcgatctg atcgttgaag ctggaggtac ccaggttgat aaccctcacc
     2701 tggggtgtaa aaggcatccc cacaaactca tagacgcggg gagccagcac ctgggagaca
     2761 tacaaatcca ccgcgggatc gctcagcacc agcacttcca tcgagtcagc cgcgctgagg
     2821 ttgttgagca ggtcggtgag ggtgacatat agctgggtgc tgccagtaaa gccattggaa
     2881 ctgaggacca ggtccagtcc gctcacgctg acgttcaggt tgggattggg ggctacagcg
     2941 atgctggtgt gggccaaatc ggtgcagacg atatggggta tgaggtccag ggtgtaggtg
     3001 tcactctcga agaaacttat ggactgaggc aggtccagtt ccggagtcac gttgaggggc
     3061 tggcctattt tgatgtcatc caccaggatg ccgtaagtgt tgcattccca gtagaaaccc
     3121 aggtagatgc tctgtcctgc ccattgattt agaggcaggc tcacctgctg gtatggggtg
     3181 ccgatgttgg tgagatggac cagaagctgg ttgagagcac tgggattggg ggccgcggtg
     3241 gatacaaaga ccttgaggtc ttcggtactc acccaggagc gggtgaagaa aaacaacgaa
     3301 tcgcccgggc tcacagtgat ttgaggggtc accagccagt cggcgttttc attgggcaaa
     3361 tagttatcca caaaagcggc acggctgccg gtgtgggcat tggcgtggct gagattgcgc
     3421 caggtcatgc catcgccgtc gtcaaagatg gtccatccgg ccgggagggt tgtgatgccc
     3481 tcgaagcctt cctctatatg ccactgtccc agcagcaggc tcgaggctgt caacagcagt
     3541 gcgatcatta atatgtgttt catgatatag ctccttacag gcttttcagg gagggttttt
     3601 ctatctggac aaaccattca cagccacccg taacctggcc accgatggaa cggttgtctc
     3661 cccaccagac tgtgaccagt ccgcgggatt tttgggaaaa agacatatcg atgttggaaa
     3721 gtgagacgtt gggattggtg cccatacaga tcagggccac cttgcgtccg tccgaacctc
     3781 cttccgcgat agctctggcc aggaggtcct gattcacggc ggcggaaaag ctgctgatct
     3841 cgccattcct gatttccagg gaaggctgga tgatcttttc tccaaaggca taggccacgt
     3901 ctcccctaat cctgccatgg gccgaagcgc tcttgggcag gatgaggact tcgccgccgg
     3961 gatagttgat cacggggctc tgacaggggc tttcgccaaa ggaaccgctt tccgccagaa
     4021 cccgttccgt ctggaaggtc agggaggcct cgtaactgct gagcttgatt tccccggctg
     4081 aggcaaagtt ggagcagatg ctcatagttt cctggctcag cttctcatag tccacgtcca
     4141 gcgctttcca aaactgcagt tcgctctcga tggagttttc gctgagcgtg tcgttcagca
     4201 gccccggcat catcgccact ctgatcttgc ggtctttgag gatgccatga ctggcggcat
     4261 aggccatggc gcggatctgt atcttttcgg gatcggaaag ctggatatga cgggcttcgc
     4321 cgttgaggtt cagataggcg tcgtgagctt tgagcagacg gttgaagtga tcgtagtctg
     4381 catggaaggc cgtgagcgag tattcctcat atttggcctt taaaagctga gaatcctggt
     4441 agatggtggt gcagcggata ttcatgcgtc cagcctccaa aaggatcttc tccaccaggg
     4501 gcaggttgta tttgccggtc acgatcaaga gcttttgccg ggaccggagc ttgagggaat
     4561 gcttcaggag tttggccgcg atggcggaaa gattgaggtt gtactcccct tccaggatgt
     4621 tgttgatcac gccccggctg ttgatcaagc cgcctccaag atgttctccc agagagaaat
     4681 ccagcacata gtttttggcg gtggcgcgat aatagacttc tttgccattg ccgggctcct
     4741 ccaccacttc gatgagaccc tcttccatca tcttggtgag attgtaatgc accttttgct
     4801 tgctgacgct gaaagcggtg gccagttgct gacaggtggc cgcgcttctg atgagctctt
     4861 tgagcatgtc aaactcgagc gcgttgccca gttctttcac ctgggagtgt ttggagatga
     4921 tctgaagatt ttttatcatt tctggcttat ccttgtttaa gcttgctgcc acccacgtag
     4981 cgtcaccatc ccctatatct gcataaacac gatattgata gtgacgaatc gggaagcaga
     5041 gccagattag gataagccta gatattgtca agaagttttt gactatatcc gcagataccc
     5101 tcagaaatgc agcggcattt cgagacggga tcaggccggg aacacgctca gttcagttct
     5161 tgccgcgaaa agttttaatc gtcaggacat ggatgcttac tcccacagcc accagcaaca
     5221 gcatgatccg gacgggccaa agtctgccgg ggatgaacag cagcgcggag ctgaggatac
     5281 cggcccagag gaggcccagg gtaaacagct tgacccgcac cgggatgcct tcccgggcct
     5341 ggtagcgacg cagatactct ccaaacaggg cattgtggtg catccaatgg tagagccttg
     5401 ccgagctcct gccataacag acggctgcca ataacagcaa aggagtggtt ggcaacaacg
     5461 gcaaaatgac tcccagcagt gccaatccca gggccaggct gccaccaatg atcagaaaaa
     5521 cccgccccag agagtttccg gggcggcgat cgttggctgg gagtcgtttg tcagacatga
     5581 gtttgagggc ttaaatgccc ttcagcactt cttccatggg aatggagcag gctttggcca
     5641 ctccttctcc gtatttggga tcggctttat ggcagttgcg gatgtggcgc agcttgattt
     5701 cgaggggagc gtctcccatg ttgcgggcgg tgttttcaaa gaggacttgt tgctgctccg
     5761 ggctcatcaa ttggaacagg gcgcgcggct gagaatagta gtccgcgtcg tcacggtggt
     5821 tccagtgatc agcggccccc gcgaggtcca gaggcggttc tttgaaatcc ggctggtcct
     5881 gccattcgcc atagctgttg ggctcgtaac ccagagtgct gccatagttt ccgtccaccc
     5941 gcatctggcc gtcgcgatga tagctgtggg cttcggcttt aggacggttg accgggatct
     6001 ggtgatgatt gatgcccagg cggtaacgtt gcgcgtcgcc ataggagaag agcctggcct
     6061 gcagcagccg gtctggtgag aatcccaggc cgggaacgat gttggcaggg gtgaaggcgg
     6121 cctgttccac atcggcaaag tagttggccg ggttgcggtt gagctccagg attcctactt
     6181 tgatgagggg ataatgcttt ttggaccaca ccttggtgat atcgaaagga ttgaagggca
     6241 cctgagtggc ctcctcttcg ctcatcacct gaatgctcat ttcccagcgg ggaaaatcac
     6301 ctttttcgat gctttcatag aggtcccgct gatggctttc acgatcggtg gcgatgatag
     6361 cttcagcttc ggcatccgtg aggttcttga tgccttgcag ggtcttgaga tggaatttga
     6421 cccacacgcg ggtgttatct ttgttataca tggcaaaagt gtggctgcca tagccattca
     6481 tgtgacgata ggaataggga atcccgcggt cgctcatcgt gatggtgacc tggtgcaggg
     6541 cctcaggcag cgaactccag aaatcccagt tgttcttggc gctcctcata ttggtgcggg
     6601 gatcgcgttt cactgcgtgg ttgaggtctg gaaacttgag cgggtcacga ataaagaaaa
     6661 cgggggtgtt gttgcccacc aggtcccagt ttccctcttc cgtgtagaat ttgacggcaa
     6721 agccgcgaat gtcgcgttcg gcatccgcgg cgccacgttc tcctgccaca gtggagaagc
     6781 gggcaaagac ctcggtctgt ttgccgatct ctgagaagat tttggccttg gtgtactggc
     6841 tgatatcgtg ggtgacggtg aaatgaccaa aagcacccga gcccttggca tgcatgcggc
     6901 gctcaggaat cacttcccga tcaaaatggg ccagcttttc caccagccac acgtcttgca
     6961 tcaagagcgg gccacgcggt ccggccgtca tgatgttttg attgtccacc accggtgttc
     7021 cggctacggt ggtcagtttc ttctttttct tcttttcatc catttccttg ctccttgtcc
     7081 tgatctgttg gtgagttgat ttcatccttg tctgccaagc atttgcggca aaggcccctg
     7141 atctctacat gcagcgattt tgcctcaccc atattgccaa gatcggcagg cagggggatg
     7201 gcgttgaact catgattgtc ataatcctgg acggcaccac agcgttcgca gacaaagtga
     7261 tgatgatggc tgtgattggc atcaaaacgg gcgacgccgt gatggggatt gagggtggcg
     7321 atgataccca aatcccggaa ggtccagaga gtgcgataaa ccgtatcgag cgagagggtt
     7381 ggaagcctct tgcgcagacg cttgtggatt tcctcggcgc tgggatggtc ctccctggca
     7441 gccagagctt tgtatatctc caggcgctgc tgggtgagct tataaccctt ctcaatcata
     7501 agcattttaa agcgttcaat ccgctgcgat acaagctgtt tattgatcat aaccattcct
     7561 aataaggatt tattcctaag gataggttaa gccatttgag gctgctgcca agcataaaaa
     7621 taccaagccc cggaatgact gtcccggggc ttggaaacat ggccaagaag cgttacttgg
     7681 tgagcaacac tcgctgagac agatattcgc ggtccacttt gaccctgatc agatagagtc
     7741 ccgggctggt cttctggccc cggtcgtctc tggtgtccca gatcacagac ttggtgctgc
     7801 cgagcgccag agtgccaaga tcgcgcacct tctgaccgcg gatattgtaa acctgcacct
     7861 gggcctggat gccagattcg ctgtccagac tgatggtgac cgcatccgag cctttgttgg
     7921 tggtcgcttt cagcttggcg ggattggtca cgatgctgaa cacggcatcg ctgatatcca
     7981 tctgaccagg agcgtgaatg gcgcggacca tcaccttata gttgtctccg gatggaacgc
     8041 caggcggtat ggtccaagcg aaactgccgg tattgggaag actggagctg atgatatgac
     8101 gatagcgctc ccgctggcct tccctgagca gccagagctg catggggacg ttgaggtcgg
     8161 gagcgttcca ggtgatcgta tagctgttgc cagcctgcca gagttctcct ccgttgggac
     8221 tggtgagcgt cacattggat ggaggcgggg cctgatgggc caagatcgtg aagggggcat
     8281 cgctgagatc ggccagattg gcgttcaggc ttctgatccg gatgcgataa tcactggcgg
     8341 cctcgagatt cataggaata tgccatctga acatcccggg aatgggcagc ccgggagcga
     8401 tcactagagc gggctgagga tcgtctcccc tcatcagggc tatttcgacg gggccttgct
     8461 gttgaggagc atgccagcgg atgggatgca tggtgccggc catccagtgc tctccaccat
     8521 tgggcgagat gactctaagt gtctgaggcg gagggggatt gccgttcacg atactaaaag
     8581 atgagtccga ccaatcctcc accagagtgc catccgccat gggagtagaa atccggacca
     8641 gatattgatt tgaaagggga agaccgctag gtatcatcca gtgaaagagg gagggaaagg
     8701 aagggatatt gaccgcgatg ggaataggtg ccaccgcagg gtccattcca tcatgcaggg
     8761 tcaaagtgac cggcaggatc gcgttgaagc cctgcaccca ggtgatggga tgcatggtgc
     8821 ccgccaccca ggtttctcca ccgttgggag atagaatctg cagcatctga ggcggaggag
     8881 gtggagtgcc attcaggata ctgaaaggac cgtcggaaaa atccatctgg atagtcccgt
     8941 ccggcgaaag cgaagatacc tgcatgtaat actgctcaga aggggccagg gtggcgggaa
     9001 tctgccagtg aaaaaaacct ggaaaagggg gtagattctg agctatcagg atagggggcg
     9061 tattggtgtt taaaccattg taaagcttca gggttacagg catggcagcg gtggaagagc
     9121 agacccagtg aatgggccgc gtcgtgcccg tctcccactg ctcagctccg ttgggagaga
     9181 tcagctgcag gttttgagga ggaggcgggt cgctgcccac gatgctgaag ggcagatcac
     9241 tcacgtcgcc aaaatagacg gtgagatcgc tgagccacat cacatggacc ctgtatccat
     9301 tgccaggcat gatatcatcc ggtaccgtcc aggtgaaatt tcccggcatg atcggcactt
     9361 gagcggcgat ctgataatcg gccacgccgt tggcctgcac taaagtgata accacatccc
     9421 cctcaaggcc ttgcgaggtc cagttgatcg tctgggtgct gcccagaggc cagatctctc
     9481 cgccattggg attctggacc gtgatctgca ccggtggagg aggaggatcg cctcccagga
     9541 tggtgaaagg cgcgtcgctg atgtcgctga agctgtttga gggattggag gccatgctga
     9601 tcatgacctt atagaaatcc cccggcatga tgctggcggg gatttgccaa tggaacagct
     9661 gcgcctgaac ggggatgttc atcgcgatcg ccaaaggtgg ctgtgagctg agcgggctga
     9721 tcagatgcag gctcaccgcg ccagcaagat tcgtctgctc ccaggtaata tcataaacag
     9781 agccggcctg ccactgttct cctccattgg gtgccgtgag caccagaggg acattttggg
     9841 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncacggcat cactacggcg
     9961 ggtgaataag caagaatgag acgcgccttc caagctgaac ttcatctaaa ggcggaattt
    10021 tgcccgtcac tctgccagca tcttacagac acgcgattag acctattgga tcaagctccg
    10081 gggcggataa aacgcaaaaa aagattttgt ccttgacaga aatcggctta tactaatttt
    10141 agcactcaac taatctgact gctaagaaaa cgaagtcaat acatgattat ggaggtaaac
    10201 tcaaatggca aaacaaatgt tgtacgcgca cgacgcccgc acttccctca agaagggtgt
    10261 ggaccagctc gcggatgccg taaaagtcac ccttggaccc aggggcagaa atgtggtatt
    10321 ggacaaaaag ttcggttccc ctaccattac taacgacggc gtgaccatcg ccaaagagat
    10381 cgagctcgaa aacgaatttg aaaacatggg cgcccaactc tgcaaagaag tggcggaaaa
    10441 gacccacgac gtagccggag acggcaccac cacggccacc atcctggctc aggccatcat
    10501 tgaagaaggc ctcaagcacg tgaccgccgg cgtcaatccc atgtatctga aacgcggcct
    10561 ggaaaaggcc agcaaagtga tcgtggacca gatccacaaa ttcagcaaag agatcaagag
    10621 cagcgaagaa gtggcccaga tagccaccat cagcgccaac aacgatcccg agatcggcaa
    10681 gctcatcgcc gaagccatgg atagcgtagg ccgcgaaggc atcatcaata tcgaagaagc
    10741 caagtccatc gacaccggcc tcgagaaagt ggaaggcatg cagtttgacc gcggctatct
    10801 ctctccttat tttgtcacca accccgaaaa gatgatcgcc gagctcgaag atcccttcat
    10861 cctgatctac gacaagaaga tcagcgtgat gaaagacctc ctgcccatcc tgcaggaaac
    10921 cgctcagacc ggcaagcccc ttctgatcat ctctgaggac atcgaaggcg aagcgctggc
    10981 caccctcgtg gtcaacaagc tgcgcggcat cctcaatgtc gtggccgtca aagctcccgg
    11041 ttttggcgat cgccgcaagg ccatgctgga agacatcagc atcctcaccg gtgccacttt
    11101 gatctccgaa gacatgggcc gcaagctgga tagctgcacc ctcaccgatc ttggccgcgc
    11161 caagaaagtg atcgtggaaa aagaaaacac caccatccgc gaaggtgccg gttcccagga
    11221 cgccatcgat ggccgcgtga aacagatcaa ggcccaggtg gaagagacca cttccgatta
    11281 tgacaaggaa aagctgcaag agcgccttgc caagctgagc agcggcgttg ccgtgatccg
    11341 cattggtgcc gccaccgaaa ccgagatgaa agaaaagaaa gcccgcgtgg atgatgctct
    11401 gcatgccacc cgcgctgccg tcgaggaagg catcgttccc ggcggtggag tcactctcat
    11461 ccaggccgcc aaagccctca aggaaatgaa agaccttagc cacgaagagc agatggccgt
    11521 ggagatcctc gtcaaagccc tggccaaacc cgcctatcag atcgccacca acgctggcga
    11581 agaaggcgcc gtgatcgtgg aaaaactcaa agaatacacc gacgtgcaca tgggctacaa
    11641 cgcagccaac ggcaagtttg aagacctctt caaggccggc atcatcgacc ccgccaaggt
    11701 ggtccgctcc gccgtccaga acgccacttc cattgccgct ctgctgctca ccaccgagtg
    11761 catcgtcacc gatatcaaag aacccgaagc tcctgcccca atgcccaatc ccggcatggg
    11821 cggaatgtac taaaacactc caataccatc catacatcca gcctctcctc cccggagagg
    11881 cttttttt
//
LOCUS       DAOSFN010000072        11848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4556_length_11848_cov_9.564911, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000072 DAOSFN010000000
VERSION     DAOSFN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4556_length_11848_cov_9.564911"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLO35_05865"
     CDS             complement(<1..242)
                     /locus_tag="PLO35_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOH79229.1"
                     /translation="MGYIGEIFVMAMTALFIQNFVLSRFLGLCPYLGVSKKIDSAFGM
                     GMAVIFVMTLTSSITYLINRYLLANFMYLQTIAFILT"
     gene            complement(242..832)
                     /locus_tag="PLO35_05870"
     CDS             complement(242..832)
                     /locus_tag="PLO35_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOH79230.1"
                     /translation="MKLLSEVTKGIIKENPIFVIVLGMCPTLAVSTSVDNALGMGAAA
                     TFVLICSNMIISLIKGIVPSSIRIPIYIVVIAAFVSIVDMSMAAFLPDLHKALGLFIP
                     LIVVNCIILGRAEAYASKNNVINSMADGLGMGLGFTLSLVIVATIREILGSGTWLKMQ
                     VMPSSYEPMLVAILAPGAFITLGFLMALMNMMKEKK"
     gene            complement(829..1437)
                     /locus_tag="PLO35_05875"
     CDS             complement(829..1437)
                     /locus_tag="PLO35_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOH79231.1"
                     /translation="MMYYIKLGGILLIICVIATGILAYVNSVTEPKIIALKAAEAVKS
                     REALIPGADFEEVKVSDDFSYFIAYKKGSKELLGYTFTAGKTGYSSKVLTMAGLDPQF
                     NIINIKVIDQAETPGLGANCTAPSFSDQFKGKTQEQMLVDKDGGPIKSLSGATITTRA
                     IANSLMEQIQVLKKDVDSHLRGNDTEIGNDVTLAKDKQEVGQ"
     gene            complement(1434..2537)
                     /locus_tag="PLO35_05880"
     CDS             complement(1434..2537)
                     /locus_tag="PLO35_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOH79232.1"
                     /translation="MKDKVIVSPAPHLHDRTSIPNVMWNVVLALTPALIASVYFWGFK
                     SLLLTLLGAVAAVCTEALIQKLRKVPIAVADGSAFLTGMLLAYNIHANAPWWLPVVGS
                     AFAIAVGKQVFGGLGNNPVNPALLGRAFLLASWPTLMTNGWVATKAGSMNGITNLKLI
                     HDNLQSIAPKAYDLVTSATPLKVAQTLRDSSFVNSLTPDPGTNLDLVNRIFIGLTDGS
                     TLQNLFWGNIGGCIGEVSVFALLIGAAWLLWKNIIEWRIPLFYLATVAVLTLLFGGIK
                     GSGVSLSVPVFHLFSGGLMLGAFFMATDYTTTPMTKKGRIIFGIGCGLLTVVIRLVGG
                     YPEGVSYSILFMNVMTPLIDALTVPKAFGKVKK"
     gene            complement(2552..3877)
                     /gene="rsxC"
                     /locus_tag="PLO35_05885"
     CDS             complement(2552..3877)
                     /gene="rsxC"
                     /locus_tag="PLO35_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOH79233.1"
                     /translation="MRLKSFPGGVHPHDHKHYSAAAKIEELAAPAKVVIHLSQHIGSP
                     STPVVAVGDEVKTGQLIAEASGFVSIPQHATISGKVTRIDRFPHPCGTKQIAIEITSD
                     GKDEWVEITDEPRFMDLPASEMKERVAAAGICGMGGAGFPTVVKLSPPADKPIDTVIL
                     NGVECEPYLTSDYRLMLERAEDVITGLKLIMKIVSAAKGAIGIEANKPDAIALMEKLC
                     AKEQNISVVPLKLRYPQGAEKQLIYAATRRRVPNAGGLPSAVGVVVQNVGTAVAVYEA
                     VRFRKPLVDRIITVTGHIVKTPKNFKARIGTLYSELVEACGGLTEEAGKYISGGPMMG
                     FAIPNLEAPVAKGSSGLVIFDKKEANILEEHPCLHCARCVDVCPLNLMPSLIAAQVKA
                     KNLDMAVRFGLNDCMKCGSCSYVCPAHIRLVQWIDTGKIRWSEAQRAKK"
     assembly_gap    3985..4084
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4646..8086
                     /locus_tag="PLO35_05890"
     CDS             4646..8086
                     /locus_tag="PLO35_05890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOH79234.1"
                     /translation="MPFVHLHNHTQYSLLDGACRVDRMIALAKEYEMPAVAITDHGNL
                     FGAIDFYKTARKAGIKPIIGIEAYIINGELGTEQSKNDPRYHLVLLAKDYSGYKNLMK
                     LSSRSYIDGFYYKPRISKSLLKQHSEGLICLSACVKGEIPTLLLNERTTEAKEAVRWF
                     LDIFKEDYYLEIQDHGLDSERQVMPKVITLAKEMGIPLVLTNDCHYLHKEDNEAHDIL
                     LCIQTGKTYNDPARMRYNTNQLYFKSPDEMKELFPDEAEAYANTLKIAEKVDLELRYD
                     EFLLPRIDTPPEYPDMGSYLKALCEEAAKHKYPDMTQEVRDRIDFELDVIHRMGFDGY
                     FLVVRDLIENSRKQDVPVGPGRGSAAGSIIAYLLDITLVDPIKYGLLFERFLNPERIG
                     MPDIDIDFCAQGRGKVIDYVVQKYGRNSVTQIITFGTLGAKSVIKDVARVLMVSATDA
                     NNITKTIPFGVKDLEEAYQQAPEFSALINGNELYQSIYKHSLVLEGLIRQTGIHAAGL
                     VIAPGDLTDYVPLACGTQKEGESTILVQYEGKWLDELKFLKMDILGLKTLTLIKKTID
                     LVKQSHGNDIDINTIDITDQKVYKLLGKGETDGIFQFESEGMRKNLIELKPNMFEDLI
                     AMVALYRPGPMRFIDTYIARKHKREKVVYDHPIMESTLKETYGVTVYQEQVMRISREM
                     AGFSRGDADTLRKAMGKKILELMAKFEKKFIEGATANQVPLNVAQKIWEDWKKFAEYA
                     FNKSHATCYALVAYQTAWLKAYYPVEFMAALLSLEDNPADVPVKIEVCKSMGIKIIPP
                     NINRSDSEFSVHGKEVLFGLRAIKNLGEAAMRAIIEERSANGPYLNLFNFCSRLDSGA
                     VNKTVLESLIASGAMDELEGSRSQKWEAIETALSFSSSEQRERRKGQTSLFDLIAEDE
                     GEEDYTPPLPTVDEWSYMHQLEMEKSVLGFYLSGHPLSQYRQSVKFYTTSCASVKREN
                     GAQDLVLAGIVTNLAKKKDAKGNPIAFVEMEDLSGRYEVPLFSRDYQDYFQIVSSGKV
                     LAVIGSRSSYNGNDDGMLRVNPQKIMPIEELPRYLKGELQLELPASKLSKALVDEVER
                     CVKSTTGQFTLRTTVQNEEDSFVLESGKRFFPENRFLEWLDKHGLSFSLRVITNGKAD
                     "
     gene            8070..9173
                     /locus_tag="PLO35_05895"
     CDS             8070..9173
                     /locus_tag="PLO35_05895"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOH79235.1"
                     /translation="MAKLISLILLLLTGLAVNAQEIIFERYEEGTDFWVIVPYNTISF
                     KKNTDRVSYQLAIEIKNSKKKVAASFARTLDLPKRDWLRDTAIPVRFTARLEPGKYEA
                     ILQLKNLVLGDKTKLKKTFTLGKLTEIGQPYLLVTREDITYLPPNLDLAGAEIQSCEI
                     VQSFAVIADSIAVEMDDRRILHVLPHSPVTTSLLHDSQLPAKLNISFYEGNIHYRMEA
                     FTYSPWFSYNLRYSFKDQLLQLRYLANQNEWKSLSALPEKQLSEGIELFWQRNDPSPG
                     TIRNEAREDFYRRVLKADELFTVHKKLKGWNSDRGRIYIKYGEPDEILTDVLPLGAYP
                     SIVWIYYKHNREFVFNDTGGYGQYILRNKDAEY"
     gene            9241..10497
                     /locus_tag="PLO35_05900"
     CDS             9241..10497
                     /locus_tag="PLO35_05900"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOH79236.1"
                     /translation="MFIESNRFLDGAGNTVMHIDYQVPYRNLMFLAHKGGYFAELKIN
                     VSMTRDDSLVVVRDLTDNVGISNKDDAVSDKTYLNRLSFLLDSQVRKLIFTALDVNSQ
                     RSFSWEFEASPLTSQDIFSDIQLCSEVLADSSSYLRSFHRGAILYKTEPSLIFDKTMV
                     PEVFLYLEIYPAPELIGQSQLLTLYLEKDSLMVRDDYIDFTPGSATEGITLKIPLEDL
                     KPGKYIGNLAMQLGEAYLEREFEFFVTESVEQQYAILTDPEEEIVLLRYFSGNQVPSD
                     WARYDDSVKQRYISSIWKRMASTGRIRPEDLLSSVRERVDYANRNFKYFKDGWKTDMG
                     RIYIRNGKPDEVEKDTTMDDTRYVRKDYQIWKYRGRLNAVYAFVDMQMNGNYQLVYVN
                     NDDMERSNPDYLRYLGDDFDTSKLGD"
     gene            complement(10529..10915)
                     /locus_tag="PLO35_05905"
     CDS             complement(10529..10915)
                     /locus_tag="PLO35_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOH79237.1"
                     /translation="MDRITQNAFFHIGEDIAAKFLLNKGYELLCKNFRIRNGEIDIIM
                     RSDTHLIFVEVKTRRFHSIDAALQTVTWTKRRNISRTAQEYINQNPDYAKLQTRFDIV
                     LVFYEQASNSFSVKHFPDAFYPVFDR"
     gene            11002..>11848
                     /locus_tag="PLO35_05910"
     CDS             11002..>11848
                     /locus_tag="PLO35_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79238.1"
                     /translation="MKRVLLSVFVLASLLSVLDAAPFQTLGMLRTPDAYVLPHKAAEI
                     LLVGYYRDVAKPSYVDPDMSDKKIVPYGMLGVGLGDRVELGFFGGDKVYYFNAKLKLI
                     QETLRIPQVSVGVDNVLSPVNRHRAQDWTPANAADWDYSDHPDKVDYEYWSPYLVASK
                     QMVFGGVNWMFNLGVGSNRFTGQVPRSRLFNGIFYSIELSPFKNFFIQGEYSGHDFNA
                     GLKYNIGNFGIKVAGQAIEDLAKDNGYEENLRLALGVSYLFDKYAKAKRRPDMRELAK
                     IDDELL"
BASE COUNT         2970 a         2927 c         3190 g         2661 t
ORIGIN      
        1 gtgaggataa aggctatcgt ctgcaaatac atgaagttgg ccagcagata gcgattgatc
       61 agataggtga tggacgatgt gagggtcatc acaaagatca cagccattcc catgccgaag
      121 gcggaatcga tcttcttgga gacacccaga tatgggcaga gacccaaaaa gcgggataac
      181 acgaagttct ggataaagag ggcggtcatt gccatcacga agatttcgcc aatgtatccc
      241 attatttttt ctccttcatc atgttcatca aggccattag aaatcccaaa gtaatgaatg
      301 caccgggagc caggatggcc acaagcattg gttcataact ggaaggcatc acctgcatct
      361 tgagccaggt tccactgccc aggatttcac gtatggtagc cacgatcact agcgagaggg
      421 tgaatcccag ccccatgcca agtccgtctg ccatggaatt gatcacattg tttttgctgg
      481 cataggcttc ggcgcgtccc aggataatgc agttaaccac tatcaatggt ataaacaacc
      541 ccagagcctt gtgtagatct ggcaaaaaag ctgccatgga catatccacg atgctcacga
      601 atgccgcgat caccacgatg tagatgggga tgcggatgct ggagggcacg atgcctttga
      661 tgagcgagat gatcatgttt gagcagatca acacaaatgt ggccgcggct cccataccaa
      721 gggcgttatc cacagaagtg gaaactgcca gagtggggca catgcccaac acgatcacaa
      781 agatcgggtt ttcctttatg atgccttttg tgacttcaga gagcagcttc attggcccac
      841 ctcctgttta tcctttgcca gggtaacgtc gttcccaatc tcagtgtcat tgccgcgaag
      901 gtgggaatct acatccttct tcaacacctg aatctgttcc atgagggagt ttgcgatcgc
      961 gcgggtggtg attgtggctc cgctaaggct tttaatcggg ccgccgtctt tatccaccag
     1021 catctgttcc tgggttttgc ccttgaactg atcagagaag cttggagcgg tacaattggc
     1081 tcccagacca ggagtctcag cctgatcgat caccttgata ttgattatgt taaactgggg
     1141 atcgagaccg gccatggtga gcactttgct gctgtagcct gtcttgccgg ctgtgaatgt
     1201 gtaacccagc agttccttgc tgcctttctt ataggcaata aaataactga agtcgtctga
     1261 taccttgacc tcctcaaaat cggctccggg aatcagagct tcacgacttt tcaccgcttc
     1321 agcagctttg agggcgatga tcttgggctc ggtaacggaa ttgacataag cgaggatgcc
     1381 ggtggcgatc acgcagataa tcagcaggat gccgccaagt ttgatatagt acatcatttc
     1441 ttcaccttcc cgaatgcctt tggaaccgtg agggcgtcta tcagaggcgt catcacgttc
     1501 ataaagagga tggaatagct cacgccttcc ggataaccgc ccaccagtct gatcaccact
     1561 gtgagaagtc cgcagccgat gccaaagatg atgcggccct ttttggtcat gggagtggtg
     1621 gtgtaatcgg tggccatgaa aaaggcaccc agcatcagtc cacccgaaaa gaggtggaac
     1681 accggcacgg agagcgaaac gccgctgccc ttgatcccac cgaagaggag ggtcaaaaca
     1741 gccacggtgg ccagatagaa gagcggtatg cgccactcga tgatattttt ccacagcagc
     1801 caggcagccc cgatcagaag ggcaaaaacc gacacttcac cgatgcagcc accgatattt
     1861 ccccaaaaga ggttttggag ggtgctgcca tctgtaaggc caataaatat cctgttgacc
     1921 agatcgagat tggtgccggg atcgggtgtc agagaattca cgaaactgct gtcccgcagg
     1981 gtctgggcca ctttaagagg ggtggcactg gtgaccagat cataggcttt gggagcgata
     2041 ctttgcaggt tgtcgtggat gagcttgaga ttggtgatac cgttcatgga accggctttg
     2101 gtggccaccc atccattggt catcagcgta ggccaggacg ccagcaggaa ggcgcgtccc
     2161 aggagggccg gattcacagg gttgttaccc aggccaccaa atacctgctt tcccaccgcg
     2221 atggcaaaag cggaccccac cactggcaac caccacggag cattggcatg gatgttgtag
     2281 gccagcagca tacctgtgag gaaggcagag ccatccgcca cggcgattgg gaccttgcgc
     2341 agtttttgga tgagggcttc ggtgcagact gccgcaacgg cacccagcag ggtgagcagc
     2401 agagatttga agccccagaa atagacggag gcgatcaaag ccggagtgag ggccagcacc
     2461 acattccaca tcacattggg gatggaggtg cggtcatgca gatgcggcgc gggggatact
     2521 attactttat ctttcataat tcgttaacct gctacttttt ggcgcgctgg gcttcagacc
     2581 agcggatctt accggtatcg atccattgca ccaaacgaat atgggcgggg cacacatagg
     2641 aacagctacc gcacttcatg cagtcgttga ggccgaatct gactgccatg tccaggtttt
     2701 tggctttgac ctgagcggcg atcagactgg gcatcagatt gaggggacac acatccacgc
     2761 agcgggcgca gtgcaggcag ggatgctctt ccaggatatt ggcttccttt ttgtcaaaga
     2821 tcaccagacc ggagctgcct ttagcaacgg gagcttccag attggggatg gcaaagccca
     2881 tcatgggacc cccggaaata tacttgccgg cttcctctgt gagaccgcca caggcttcca
     2941 ccagttcgct gtaaagggtc ccgatcctgg ccttgaagtt tttgggcgtc ttcacaatat
     3001 gaccggtgac ggtgatgatc ctgtccacca gcggtttgcg aaaacgcacg gcctcataga
     3061 cggccacggc tgtgcccacg ttttgcacca ccactcccac cgcgctggga aggcctccgg
     3121 cattggggac gcgacgacgc gtggcagcgt aaatgagctg tttttcagcg ccttggggat
     3181 agcgcagctt gagcggtacc acgctgatgt tctgctcttt ggcacaaagc ttttccatca
     3241 gagcgatggc gtccggcttg ttggcttcga tgccgatggc acctttggca gcggagacga
     3301 tcttcatgat cagtttaagc ccggtgatca cgtcttcggc tctttccagc atcaacctgt
     3361 agtccgaggt gaggtagggc tcgcattcca cgccgttgag gatcacggta tcgattggtt
     3421 tatcggctgg gggagatagc tttaccactg ttgggaagcc cgctccgccc attccgcaaa
     3481 ttccggcagc ggctacgcgc tctttcattt ctgaggctgg aaggtccata aatcggggtt
     3541 cgtcggtgat ttccacccat tcatccttgc cgtcggatgt gatctcgatg gcgatctgtt
     3601 tggtgccgca gggatgcgga aaacggtcga tcctggtgac tttgccgctg atcgtggcat
     3661 gctggggaat ggaaacaaaa cctgaggctt cggcaatgag ctggccggtc ttgacttcgt
     3721 cacccactgc cacaaccggt gtggacggag aacctatgtg ctgggataaa tggatgacca
     3781 cctttgccgg agcagctagt tcctcgatct tggccgccgc ggaatagtgt ttgtggtcat
     3841 gaggatgcac ccctcccgga aacgatttca acctcattaa cgctcctgtg gactcaccaa
     3901 agatgagcca gattattttt tggtgcccaa aatcctggca ggggcataaa aggcaagttt
     3961 ttttttgggg gagaggcaga gtcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnntcagcc tcctttacac cacctatgcc tctcctatgc ctcacctgtg cttaggcatg
     4141 ggaggggcac aggagaggtg caggtgtgga aaacctggca tggcagcttg gtgagtgcgt
     4201 ctatgttact gtaaaaatga ctaatagggc tattgacatc attgtccccc gccccaaaaa
     4261 aagaaacccc tccggaggga ggggttacag gtttgactta ggaggttgcg tcgccccggg
     4321 ttgaggagga cccaaggctt aaggcagggc tgccttttat ggactcacga tatagtaggg
     4381 agcaaattac gtgccaattg catgtaaata gctataagtc tattgggcag atagttacgg
     4441 aatggagaga tcgatttgaa ggcggtaacg cgtggaaata aaaaggcaaa tatgtggaaa
     4501 agattccaca tgaatggaga tcgtgaggtg tggagaggga cagatcaatg gtgtgattct
     4561 gcctccttcc caatgcggca atttatcctt tgacaaaaac ccgcgggatg ctagtttgcc
     4621 atccagaaga atcgggagag acctaatgcc ttttgtacat ctgcataacc atacgcaata
     4681 cagtttattg gacggtgcct gccgggtgga ccgaatgata gctctggcta aagaatatga
     4741 gatgcctgcc gtggcgatca ccgaccacgg taatctcttt ggggcgatag acttttataa
     4801 gacggcccgc aaggcaggga tcaaaccgat catcgggatt gaggcttaca tcatcaacgg
     4861 cgaactgggc accgagcaga gcaaaaacga tccgcgttat catcttgttt tgctggcgaa
     4921 agactacagt ggctacaaga atctcatgaa actctcctcc cggtcctaca tcgacggttt
     4981 ttactataaa ccccgcatct ccaagagcct gttgaaacag cacagcgaag gtctgatctg
     5041 cctgagtgcc tgcgtcaagg gagaaatacc cacgctgctc ttaaacgagc ggaccacgga
     5101 agccaaagag gcagtaagat ggtttctgga catttttaaa gaggactact acctggagat
     5161 tcaggaccac gggctggatt ctgagcggca ggtgatgccc aaagtgatca ctctggccaa
     5221 agagatgggc atcccgctgg tgctcaccaa cgactgccat tatctgcaca aggaagataa
     5281 cgaggcccat gacatcctgc tctgcatcca gaccggcaaa acctataatg atcccgcgcg
     5341 catgcgatac aataccaatc agctttattt caagagcccc gatgagatga aagagctgtt
     5401 tccggatgag gcggaggcct atgccaacac cctgaaaatc gcggaaaaag tagatttgga
     5461 gctccgatac gacgaatttc tgctgcccag aatcgatacc ccgcctgaat atccggacat
     5521 gggatcatac ctgaaagcgc tctgcgaaga ggcggccaaa cacaaatatc cggacatgac
     5581 ccaggaagtg agagaccgca tagattttga gctcgatgtg atccaccgca tgggctttga
     5641 tggctacttt ctggtggtgc gcgacctgat cgaaaactcc cgcaaacagg acgtacccgt
     5701 gggtccgggt cgtggatcgg cagcgggcag catcatagcc tatctgcttg atatcacact
     5761 ggttgacccc atcaaatatg gcttgctgtt tgagcgtttc ctcaatccgg agcgcatcgg
     5821 catgccggat atcgatatag acttttgtgc ccagggccgc ggtaaggtga tcgactatgt
     5881 agtgcagaaa tacggccgta acagtgtgac ccagattatc accttcggca ctttgggagc
     5941 taaatccgtg atcaaggacg tggccagggt gctgatggtc tcggccaccg atgccaacaa
     6001 catcaccaag accattccct ttggggttaa agaccttgag gaagcttatc agcaagctcc
     6061 ggaattctcc gctttgatca acggcaacga gctctatcag agcatctaca aacacagtct
     6121 ggtgctggag ggcctgatcc gtcagaccgg tatccacgcg gccgggctgg tgatcgcgcc
     6181 aggagatctg acagactacg tgcccttggc ctgcggcact caaaaagaag gggaaagcac
     6241 catccttgtt caatacgagg gcaagtggct ggatgagctg aagtttctca agatggacat
     6301 cctgggcctc aaaaccctca ccctcatcaa gaaaaccatc gatctggtga agcaatcgca
     6361 cggcaatgat atagacatca atactatcga tatcacggac cagaaggtgt ataagctgtt
     6421 gggaaaaggg gagacggacg gaatctttca gtttgaatcg gaggggatgc gcaaaaatct
     6481 catcgagctc aaacccaaca tgtttgaaga cctcattgcc atggtggccc tctaccgtcc
     6541 cggcccgatg cgtttcatcg atacctacat cgcccgtaaa cacaagcggg agaaggtggt
     6601 atatgatcat cccattatgg agagcaccct caaggagacc tatggcgtca ccgtctatca
     6661 ggaacaggtg atgcgcattt ccagagagat ggcaggattc tccagaggtg acgcggacac
     6721 cctgcgtaag gccatgggca aaaagatcct ggagctgatg gccaagtttg aaaagaaatt
     6781 catcgaaggt gccactgcca atcaggtgcc gctcaacgtg gcccagaaga tttgggagga
     6841 ttggaagaaa ttcgccgaat atgccttcaa caaaagccac gccacctgtt acgctttggt
     6901 ggcttatcag accgcctggt tgaaagccta ttatccggtg gagtttatgg ccgctctgct
     6961 ctcactggaa gacaatcctg ccgatgtgcc tgtcaagatc gaagtatgca agtccatggg
     7021 catcaagatc attcctccca atatcaaccg cagcgacagc gaattcagcg tgcacgggaa
     7081 agaggtgctg tttgggctga gggcgatcaa gaatctcggc gaagcggcca tgagggccat
     7141 cattgaagag cggagcgcca atgggccata tctgaatctc ttcaatttct gctcccgtct
     7201 ggatagcggc gcggtgaata aaaccgtgct ggaaagcctg atcgcttcgg gtgccatgga
     7261 tgaactggaa ggcagccggt cccagaaatg ggaagccatc gagacggccc tttcattcag
     7321 ttccagcgag cagcgggagc gccgcaaagg gcagaccagc ctctttgacc tgatcgctga
     7381 agatgaggga gaagaggatt acactccgcc tctgccaacc gtggatgagt ggagctatat
     7441 gcatcagctc gaaatggaga aaagcgtgct ggggttttat ctcagcggtc atcccctcag
     7501 ccagtatcgt caatcagtaa agttttatac cacctcctgc gcttccgtca aacgggaaaa
     7561 tggcgcgcag gacctggtcc tggcagggat cgtgaccaac cttgccaaga aaaaggacgc
     7621 caagggcaac cccatcgcct ttgtggaaat ggaagacctc agcgggcgtt atgaagtccc
     7681 cctgtttagc cgtgattatc aggattattt tcagatcgta tccagtggca aggtgctggc
     7741 cgtgatcggc agccgctcat cctataacgg caatgacgac gggatgctga gagtgaatcc
     7801 gcaaaagatc atgccgatcg aggaattgcc ccgctatctg aaaggagagc tgcaactgga
     7861 gcttcctgcc tccaaactca gtaaagcttt ggtggacgag gtggagcgct gcgtcaaatc
     7921 cacgaccggc caattcacgc tccggacaac ggtccagaac gaggaagaca gtttcgtgct
     7981 ggaatccggc aagcggttct ttccggaaaa ccgctttctg gagtggctgg acaaacacgg
     8041 actgagtttc agcctgagag tgatcacaaa tggcaaagct gattagcctg atcctgctac
     8101 ttttgacggg cctcgcggtc aacgctcagg agatcatttt cgagcgctat gaagagggga
     8161 cggacttttg ggtgatcgtg ccctataaca ccatctcgtt caaaaagaac acagatcggg
     8221 tctcctatca actggccatc gagatcaaaa actccaaaaa gaaagtggcg gccagttttg
     8281 cgcgtaccct ggacctgcca aagcgagact ggctaagaga tacggccata cccgtgagat
     8341 tcaccgccag gctggaaccc ggaaagtatg aggcgatcct gcagctcaaa aacctggtgc
     8401 tgggtgataa gaccaagctc aagaagactt tcactcttgg caaacttact gagataggac
     8461 agccttatct gctggtaacc cgggaagata tcacctacct gcctccaaac cttgacctgg
     8521 cgggagcgga aatccagagc tgcgagatag tgcagagctt tgcggtgata gcagacagta
     8581 tcgcggtgga gatggatgac agacgtatcc tgcatgtgct tccacacagc ccggtaacaa
     8641 cttcgctttt gcatgattcc cagctgccgg ccaagctcaa catcagtttc tatgagggta
     8701 acatccatta ccggatggag gctttcacat atagcccctg gttttcctac aacctgcgct
     8761 acagcttcaa ggaccagttg ctgcaattgc gttaccttgc caaccaaaac gagtggaaaa
     8821 gtctgagcgc tctgccggag aaacagcttt cggaggggat agagctcttc tggcaaagaa
     8881 acgatcccag tcctggcact atccgcaacg aggcccggga agatttttac cgtcgggtgc
     8941 taaaagcgga cgagctcttt acggtgcaca aaaaactgaa gggatggaac tcggacagag
     9001 gtagaatata catcaaatac ggcgagcctg acgagatcct cacggacgta ttgcctctgg
     9061 gagcttatcc ttccatcgtg tggatctatt acaaacacaa ccgggagttt gtatttaacg
     9121 atacaggtgg ctacggccaa tacatattga ggaacaaaga tgcggaatac taacagatgg
     9181 atggcgactt tgcttttgct ggcaacagct tgtggcctgg cggcacaaaa caggctgaac
     9241 atgtttatcg aatcaaaccg gtttctggat ggggccggca ataccgtgat gcatatagac
     9301 tatcaggtgc cttatcgcaa tctgatgttt ctggcccata aaggaggcta ctttgcggag
     9361 ctgaagatca acgtcagcat gaccagagac gattctctgg tggtggtgag agatctgacg
     9421 gataatgtgg ggatatccaa caaagacgac gcggtgtctg acaagaccta tctcaaccgc
     9481 ctcagtttcc tcctggattc tcaggtccgc aagctgatct tcacggcttt ggatgtgaat
     9541 tcccaacgca gttttagctg ggagtttgag gcctcgcctc ttaccagtca ggatattttc
     9601 agtgatattc agctttgttc cgaggtgctg gcagacagca gttcatacct cagaagcttc
     9661 caccggggag cgattcttta taaaacagag ccctcattga tcttcgataa aaccatggtc
     9721 cccgaggtct ttctctatct ggagatatat cccgcccccg aactgattgg gcaatcacag
     9781 ctgctcaccc tatatctgga gaaggacagc ctgatggtgc gtgacgatta catcgatttt
     9841 acgcctggct ctgctacgga agggatcacc ttgaagattc ccctggagga tctgaaacca
     9901 ggcaagtata tcggaaacct ggctatgcag cttggcgaag cctatctgga acgcgagttt
     9961 gagtttttcg tcacggaaag cgtggagcag cagtacgcga ttttaaccga cccggaggaa
    10021 gaaattgtgc tgttgcgcta tttcagcgga aatcaggtcc cgtctgactg ggccagatat
    10081 gatgactctg tcaaacagcg ctatatctct tccatttgga aacggatggc ctccacgggg
    10141 agaataagac ccgaagacct gctgtccagc gtgcgggaaa gagttgatta tgccaatcgc
    10201 aatttcaagt acttcaagga cggctggaaa actgacatgg gcaggatata catcagaaac
    10261 gggaaaccgg acgaggtgga aaaagacacc accatggacg acacccgcta tgttcgcaag
    10321 gattatcaaa tctggaaata ccggggaagg ctaaacgcgg tttacgcgtt tgtggatatg
    10381 caaatgaatg gcaactatca gcttgtctat gtgaacaacg atgacatgga aaggtctaat
    10441 ccggattatc tgagatatct tggcgatgac ttcgatacct caaaactggg agattaacac
    10501 gaatcaggca aacagccgga taactttttc agcgatcgaa aacagggtaa aaagcgtccg
    10561 ggaagtgctt gacagaaaag ctgttgctgg cttgttcgta gaacacgagc acgatatcaa
    10621 agcgagtttg cagtttggcg taatcgggat tctggttaat gtattcttgt gccgttctgg
    10681 agatgttcct gcgtttggtc caggtgacag tctgcagggc ggcatctata gagtgaaatc
    10741 gacgtgtttt cacctcgaca aagatgagat gagtatcact gcgcatgatg atatcaattt
    10801 ccccgtttcg gatgcggaaa tttttacaca gcagttcgta acctttgtta agcaggaact
    10861 tggcggcgat atcttcgccg atgtgaaaaa aagcattttg cgttattctg tccatggggc
    10921 aagaaaacgc ttgacgtatt tcaagtcaac gatattttgt gaaccacaga ttagaaacaa
    10981 caccgaaagg tggagggaag tatgaaaaga gtattattaa gtgtatttgt gctggcatcg
    11041 ctattgagcg tgttggacgc ggccccgttc caaaccctgg gaatgctgcg cacaccggat
    11101 gcctatgtgt tgccccacaa ggcggcggag attctgctgg tgggttatta tcgggatgtc
    11161 gccaagccaa gttatgtgga cccggacatg agcgacaaga agatcgtccc ctacggcatg
    11221 ctgggagtag gcttgggaga cagagtcgag ctgggattct tcggtggaga caaagtctat
    11281 tatttcaacg ccaagctcaa gctcattcag gagacgcttc gcatccctca agtgtctgtg
    11341 ggggtggaca acgttctctc tcccgtaaac cgtcacagag ctcaggactg gactcctgcc
    11401 aacgcagctg actgggatta ttccgatcac cccgacaagg tggactatga atactggtct
    11461 ccctatctgg tggcctccaa acagatggtc ttcgggggcg tgaattggat gtttaacctg
    11521 ggtgtcggtt ccaaccgctt caccggacag gttccccgct ccaggctttt taacggcatc
    11581 ttctattcga tcgagctttc tcccttcaag aacttcttca ttcagggtga gtatagcgga
    11641 cacgacttta atgccggact caaatacaat atcggcaatt ttggcatcaa ggtagccggt
    11701 caggcgatcg aagacctcgc caaggacaac ggctacgagg aaaacctccg gctggcactg
    11761 ggcgtatcct atctcttcga taaatacgcc aaagccaaac gtcgccctga catgagagag
    11821 cttgccaaga tcgatgatga gcttctgc
//
LOCUS       DAOSFN010000073        11763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4604_length_11763_cov_9.133926, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000073 DAOSFN010000000
VERSION     DAOSFN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4604_length_11763_cov_9.133926"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..2206)
                     /locus_tag="PLO35_05915"
     CDS             complement(422..2206)
                     /locus_tag="PLO35_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH79239.1"
                     /translation="MNLRDILSPFYVWKRAFEKPYSINTLSAPRPGAPRYRGFHKNDL
                     DKCIGCGTCAEICQNATIDMVPVREAKDGDSGLRPRVDYGRCCWCALCVDICPSGSLT
                     MSNAYTWVEEGLDLDVRETYCYIPGHDPKPWDNEELGYQRPEGYELLDLKRIPMPHLH
                     PEERLGSFIEMVKGYSRELAIMEADRCIQCGICTATCPAHMNIPSYIKAVREDNIAEG
                     LRILYDTNPLPGVCGRICTRRCEDVCALSHRGEAISIRWLKRYLTDCVEAKDYTRILE
                     QEVTPTGKNVAIIGSGAGGLSAAYYLRRMGYEVNIYEQNGAAGGALRYGIPEYRLPYD
                     KLDKDVDFITSLGVNIHYNTKVGHDINFDAIYEKHDAVFISTGLFEPYHIGVQGDDLP
                     MVISGLKVLADVTNQVPIELGNHVIVVGGGNVAMDAARTSRRLGSEVTVLYRRRLEDM
                     PADKEEIDEAMEEGVRFIFQAVPTGIEALSDGKARFIYSLAEMVDQGDGKRPQPIPIS
                     GSRETLDTDTVISAIGQGMNFNWMDGEAFAKIKFGKWSLEADRWGQTSDTKVFIGGDM
                     FNRTADAITAIADGHRSARAIDKVLSGS"
     gene            complement(2211..3368)
                     /locus_tag="PLO35_05920"
     CDS             complement(2211..3368)
                     /locus_tag="PLO35_05920"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOH79240.1"
                     /translation="MDTQYPPIVNGASQYDLDSGKYVRVWQGPQHPGVTGNMSLELDI
                     CGDQVVWCKTHVGYLHRGFEKLMERRRYINCFPIVCRICVPEPDTNEYLFAAAVEDLL
                     GIEVPEKAKWLRTLNLELSRLGSFLMWIGGQSGAFGMGTVAQWSITFRDYVLDLFEEL
                     SGARVYHMYMIPGGVRGDLPDGWKGRTLELMTRVEKLLDEIRHVMLNNAIFKQRSVGL
                     GIVTADMVDRFGVVGPVARGSGMKRDVRKDSPYLIYDQLDFEAVTEDGCDAYARTLVR
                     WREMYQSIELIRQILDRMPTEGEVHLKLPNVLHLKVPAGETYVRAESTRGEYGYYMCS
                     DGSAFPRKVTVRGPSYCHAMSLLEHLIVGVNIADVHGLMLSLHTYPPEIER"
     gene            complement(3370..3894)
                     /locus_tag="PLO35_05925"
     CDS             complement(3370..3894)
                     /locus_tag="PLO35_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOH79241.1"
                     /translation="MKELYELLKDRFRLSDFSQKQPVQAWLKVPSDQAVALITHLRDI
                     HGFSHLVMLTCVDWIEQGKFQLTYILHNYHDHKDLGVKVDIDRESASMESIHHLWEQA
                     RTYQRELYEMFGISFPGSPGLTDPFILEGWDDMPVMRRDFDTKAYSETHYSHRPREIH
                     EPEEHMKQRMYPED"
     gene            complement(3901..4644)
                     /gene="nuoB"
                     /locus_tag="PLO35_05930"
     CDS             complement(3901..4644)
                     /gene="nuoB"
                     /locus_tag="PLO35_05930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOH79242.1"
                     /translation="MIEDKDLDIQEETPPPESVIPAEAGTDPTEPASRYPFHNTEWRS
                     EDCKLRNEGLPEEERDWFADARPQPARTSNQIMEKFFNWCRAHSLWILAFGTGCGAIE
                     LRPLVTSRYDISRLGMQMRPTPRQASVFVIGGYVSIKTLKRIVRSYEQMQGPKFVVAV
                     CSCAINGGMYWDSYNTIKRIDEYIPVDVYVTGCMPRPEAVMAGFTKLKELIMAGKAEG
                     QNLYAEKFDWYKANQKQVIKDWNMPDYNW"
     gene            complement(4653..5561)
                     /locus_tag="PLO35_05935"
     CDS             complement(4653..5561)
                     /locus_tag="PLO35_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOH79243.1"
                     /translation="MSLTLRLVWAFISLGVVTVWGLTLTGIVAKIFARVQGRLGPPVW
                     QPFIDIIKNNAKRTAITHGVMFFLGPVFRIAGGIGTYMFIPVLFGNIYFSNFSMSGDL
                     LLVMYFIFFGQLGMALGAGEGGHPYSAIAVARGLAQMTAFEVPFALSVISIAIQYGSL
                     NIIDIVAAQQGGFLTWTLFTNPLAVIAAMIAMLGMNMHNPFSLVIAPNEIPMGPPTEY
                     QANYLGLLATNRGIFNGAKLVLFMNLFFGGATNIPVLMAKTFGIYMFSVFVGAAFPRF
                     RAEDSIRFFLKYPTLIGIVAVVIWTI"
     gene            complement(5558..8704)
                     /locus_tag="PLO35_05940"
     CDS             complement(5558..8704)
                     /locus_tag="PLO35_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOH79244.1"
                     /translation="MVNPLYLIALFLASAFAIALFDKVKREIGLGLLYATLAAALVLV
                     GFRLWELSQNPALQSQFFTAGFEVPLSIGLSFGFQEVILLIVVLVMGFLGSIAFGRRF
                     FLDGAQPMALYMLLLLGASGLILTRDIFNAFVFMEILSIASYALIGIRQTRTSLASGI
                     KYMMAGGISSALLLIGIIFAYRFTGTLNLDMWILNPVAESLAYRVALFLIAMAVFIDL
                     KPFPANGWALDVYQSAHGGMGAILSSVHSTALIFLMYKLLPLMDYGMLALFAGAGLVS
                     FVMANLIALRQTDAKRLLGYSSTAQTGLLIFIIYGLSNLGVPGYYSLVIAIGFIISNS
                     LAKGGLFWLSEMVEGKSLKSWSALKADSGLLICFGIFTIALVGLPPFPGFFAKWELVR
                     VLFTIDQPYLVIPILIGSLLEAFYMFRWLGFAAKGVSPEGYRPGAHRYMPVVLGVLTL
                     FVVSYLIPTGLLKLPVNSLYPVLGLLVFSLLDFFPARLKGLMAILGLGAYGYLQVWPV
                     LPPMGQLFAVILVGGAIVQIFGFMHRWGKAEGLFPVLMMLILSLANLLTAGSKLEFFL
                     AWEFMTVSSWMLIMRGRHARAAGLSYITFSLGAAFLIMGGLMLMPEFPLTYPLIWQLS
                     FVQIGFTPLVLMLAGFLIKMGALGVHVWLPGAYAEAEDDSTALISSVLSKAGVFGMVL
                     VLGMSVKSIGTGVLPIALGWIGVFTALGGALMAVFQEDVKKLLAYSSMSQLGYVVTGL
                     SLMTHLGWMSALYLALTHMMFKALIFMAIAGVIYRTGTRKLYEMGGLIKKMPISFISV
                     LMGIIAVSGVPPLTGFGSKWFTYTALIEQGRYLQTAVAFFSSAVAFLYLYKLIHTVFL
                     GQPKPSQAHVKEAPIWFLIPQGVFIMLIMGASMFPNLITQPISKAIADFIPKPEWLSW
                     TGYNISLASPNLQAYWNGNLVMYVTMGVFMVPLIWLMLVIAKPQKVKQFNIVYAAERP
                     YKPWTTHVAYNMFAHYQKALGSWVKPRAVSFWNGVGEWSQSLGATFGRLYTGNGQTYL
                     LHIFLYTIVMFMILGVIK"
     gene            complement(8698..9069)
                     /locus_tag="PLO35_05945"
     CDS             complement(8698..9069)
                     /locus_tag="PLO35_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HOH79245.1"
                     /translation="MILLITGMLLILAGIWAILVKKDIIRMIIGFTVVDTGVHLLIVA
                     IGYVKGGTAPIIDRAVSTADASFAGMQAISKVVDPVPSALVLTAIVIGLAVTGLMLAY
                     AVRLNKATGSTNIDDHEEQKW"
     gene            complement(9066..>9517)
                     /locus_tag="PLO35_05950"
     CDS             complement(9066..>9517)
                     /locus_tag="PLO35_05950"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOH79246.1"
                     /translation="LSSSAVKTRTEGSEILKTGSNLLVPMIVVLGIYIFTHGHLSPGG
                     GFQGGVVIASGILMLIMAEVRFHLSHLLLHISESLSGLAYVAMGLAGLILLGVHHFLD
                     PRYLLAGKFLALFSAGAVPVIYSLIGIKVGSELASVLASIQHEGEDL"
     assembly_gap    9518..9617
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9618..10006)
                     /locus_tag="PLO35_05955"
     CDS             complement(<9618..10006)
                     /locus_tag="PLO35_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79247.1"
                     /translation="MIKYLLVFAAILGMGWILWQLGVEFQTAQELNPLASEYVRGSTS
                     DLNMPNAVTAVVVSYRGLDTLGEVTVLFLATAGIGFLLKRKKRSAGLEIDDADGAANV
                     PVCDESALAGLEFDATGTTDPQNNLSNS"
     gene            complement(9999..10256)
                     /locus_tag="PLO35_05960"
     CDS             complement(9999..10256)
                     /locus_tag="PLO35_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOH79248.1"
                     /translation="MFETITLIFLGLISVAAAIFAIYTKRVVSAVIASGVISLSASIM
                     FLLAAAPDVAMTEATIGAGLTTVIFLYALKHIRASREGKDD"
     gene            complement(10249..10599)
                     /gene="mnhG"
                     /locus_tag="PLO35_05965"
     CDS             complement(10249..10599)
                     /gene="mnhG"
                     /locus_tag="PLO35_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOH79249.1"
                     /translation="MQIAGAIVTLLGSILLFLGGLGVLRMPDVYNRMQAGTKATSLGS
                     ILVLIGIGLILPAWLPRLILLVLFILFTNPISSHAMSRASHRLGIKLTDRSVRDELAE
                     RGDEAFEDGEECNV"
     gene            complement(10599..10868)
                     /locus_tag="PLO35_05970"
     CDS             complement(10599..10868)
                     /locus_tag="PLO35_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOH79250.1"
                     /translation="MDSTVMLLMQSSFYIMLAGMLLAFIRFLKGPTCADRVVALDVMT
                     VLGISVIAMLALHAGRIIYLDVALVYGLLSFLGGIVVARYMERGL"
     gene            complement(10861..11355)
                     /locus_tag="PLO35_05975"
     CDS             complement(10861..11355)
                     /locus_tag="PLO35_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOH79251.1"
                     /translation="MHKLKAFVLSTVLLFALWIALGGTARDELIVAAIVAPLISLIFL
                     PRISQLGDLRLTPLSLIYIPVYILVFLRELMKSTLDVAFRVVSPSLPINPGIVKVKTR
                     LKSPLGRIVLANSITLTPGTMTVETDGEDLYIHWIDIQSSDVDDATRKIVSTFEKYLE
                     VIFG"
BASE COUNT         2858 a         3260 c         3064 g         2481 t
ORIGIN      
        1 tacgatgata atgagttata attatatgca tttatctatc taaactgccg tccctgcagg
       61 actcatcttt gtttgggaca ccagatctat ctaaactgtc gtcccgctgg gactcgtaat
      121 gaataatgcc acgataatca gttttttcac aagctcttca gccagtgata caattattga
      181 acagtcttgt atatacatgg cagatgttgg caggcttaag atcgccccaa tcagtcaaat
      241 ctgacccgtt ggagttcaag cggccacgat aagagtatat atggcagcga gtttgctggc
      301 cgcttggact tcaagcagta catcggaggt atttatctcg cgggtgtgct gcacgcaagt
      361 ccagactttc tcagatcatg gacaagccgg atttaacgga acgaggcttg aagtgtatta
      421 tctaactacc gcttagcacc ttgtctatcg ctctggcact gcgatggccg tccgcgatag
      481 ctgtgatggc atccgcggtg cggttgaaca tatcaccgcc gatgaagacc ttcgtgtctg
      541 aagtctgacc ccaacggtca gcttccaggc tccacttgcc aaacttgatt ttggcaaagg
      601 cttcgccgtc catccagttg aagttcatac cctgaccgat agctgagata accgtatcgg
      661 tatcaagggt ttcacggctg ccggaaatgg gaatgggctg cggacgtttg ccgtcaccct
      721 ggtccaccat ttctgccaga ctgtagatga aacgggcttt gccgtccgaa agcgcttcga
      781 tgccagtggg cacggcctgg aagataaatc tcacgccttc ttccatggct tcatcgattt
      841 cttccttatc ggccggcatg tcttccagac ggcgtctgta gagcaccgtg acttcagagc
      901 ccaggcgtct ggatgtgcgg gcggcgtcca tggccacgtt tccgcctccc accacgatca
      961 cgtgatttcc gagctcgatc ggcacctggt ttgtgacgtc agccaggacc ttgaggccgg
     1021 agatcaccat gggcaggtca tctccctgca cgccaatgtg atagggctca aagagcccag
     1081 tgctgatgaa gacggcgtca tgcttctcat agatggcgtc aaagttgatg tcgtggccta
     1141 ccttggtgtt gtagtggata ttaacgccta aagaagtgat gaaatccacg tctttatcga
     1201 gcttgtcata aggcagacgg tattccggaa tgccgtagcg gagtgctcct cccgcggccc
     1261 cgttttgctc atagatgttc acctcatagc ccatgcgcct caggtagtag gcggcactca
     1321 gtcctcccgc tccggaaccg atgatagcta cgttcttgcc ggtgggtgtc acttcctgct
     1381 ccaggatcct ggtgtagtcc ttagcttcca cgcaatccgt gagatagcgt ttcagccagc
     1441 gaatgctgat cgcctctccc ctgtgagaca gagcacagac gtcttcacac ctgcgggtgc
     1501 agattcttcc gcaaactcct ggcagggggt tggtgtcgta gaggatcctg aggccctcag
     1561 cgatgttatc ttcgcggacg gcctttatat aggaaggtat attcatgtga gcgggacagg
     1621 tggcggtgca gatgccgcat tggatgcagc ggtctgcttc catgatggcc agttctctgg
     1681 aatagccttt caccatctcg atgaaagagc caagccgctc ttccggatgc agatgcggca
     1741 tgggaatccg cttaaggtcc agaagttcat aaccttccgg acgttggtag cccagttctt
     1801 cgttatccca gggcttggga tcgtggccgg gaatatagca gtaggtttcc ctcacatcca
     1861 ggtccaggcc ctcttccacc caagtataag cgttgctcat agttagcgaa ccgctggggc
     1921 agatatccac gcagagggcg caccaacagc aacgaccgta atcgacgcgg ggcctcagac
     1981 cgctgtctcc atcttttgct tcccgcacgg ggaccatgtc gatggtggca ttctggcaga
     2041 tttccgcgca ggtaccgcag cctatacact tgtccagatc gtttttatga aatcctctgt
     2101 aacgtggagc accgggtctg ggagcactga gggtgttgat ggaatagggc ttttcgaagg
     2161 cgcgtttcca tacgtagaag ggtgaaagta tatctctcag attcatgatc ttacctctcg
     2221 atctccggtg gataggtgtg cagggaaagc atcaggccat gcacgtccgc gatgttgacg
     2281 cccacgatca gatgttccag cagcgacatg gcgtggcaat aggaaggccc ccgcacggtg
     2341 accttgcgag ggaaggcgga accgtctgaa cacatgtagt agccgtattc tccgcgggtg
     2401 ctttcggccc ggacgtaggt ctccccagcc ggcaccttca gatgcaacac gttgggcagc
     2461 ttgaggtgga cctcaccctc tgtgggcatg cggtccagga tttgacggat cagttcgata
     2521 ctctgataca tctctctcca gcgcaccagg gtgcgggcat aagcatcgca accgtcttcc
     2581 gtaaccgctt caaaatcaag ctgatcatag atcagatagg ggctgtcctt gcgcacgtca
     2641 cgcttcattc cactgcctct ggccacagga cccaccactc caaagcggtc caccatatcg
     2701 gctgtaacga tgccgaggcc cacagagcgt tgcttgaaga tagcgttgtt gagcattacg
     2761 tggcgaatct catccagcag cttttccact ctggtcatca gttccagggt gcggcccttc
     2821 cagccatcgg gaaggtcgcc tctcactcct ccgggaatca tatacatgtg ataaacacgg
     2881 gcaccgctga gttcttcaaa gaggtccagc acatagtcac gaaaggtgat gctccactgg
     2941 gccacagtac ccatgccaaa ggcgccgctc tggccaccga tccacatcag gaagctgccc
     3001 agacggctga gctccagatt gagggtcctc agccatttgg ccttttcggg aacttcgatt
     3061 cccaaaaggt cttccaccgc ggcggcaaag agatattcgt tggtgtcagg ttccggcacg
     3121 cagatacgac agacgatcgg gaagcaattg atatagcggc ggcgctccat gagcttttca
     3181 aaaccgcggt gcagatagcc cacgtgggtc ttgcaccaga ccacctgatc tccacagata
     3241 tccagctcca ggctcatgtt tcctgtcacg cctgggtgtt gggggccctg ccagacgcgc
     3301 acgtatttac cgctgtccag gtcgtattgg ctggctccgt ttacgatggg gggatattgt
     3361 gtgtccatct caatcctccg gatacatgcg ttgtttcatg tgctcttccg gctcatggat
     3421 ttcacgcgga cgatggctgt aatgggtttc gctgtaggcc tttgtgtcga aatcgcgtct
     3481 catcaccggc atgtcgtccc atccttccag aataaaggga tcagtaaggc ccggactacc
     3541 tggaaagctg atgccaaaca tttcatagag ctcacgttga taggtgcggg cctgttccca
     3601 gagatgatgg atgctttcca tgctggctga ttctctgtcg atatctactt ttacgcccag
     3661 gtctttgtgg tcgtgatagt tgtgcaggat gtaagtaagc tggaacttgc cctgctctat
     3721 ccagtccacg caagtgagca tcaccagatg gctgaatcca tggatgtctc tcagatgggt
     3781 gatcagggcc accgcttggt cggatggcac tttcagccag gcctgaacgg gttgcttctg
     3841 gctgaagtca gatagccgga agcggtcttt cagtagttca tatagttctt tcatcatcac
     3901 ctaccagttg taatccggca tgttccagtc tttgatcacc tgtttctgat tggctttgta
     3961 ccagtcaaac ttctcggcat agaggttctg gccctcagcc ttgccggcca tgatcagctc
     4021 cttgagcttg gtgaaaccag ccatcacagc ttccggacgc ggcatgcaac ccgtcacata
     4081 gacatccaca ggaatgtatt cgtcgatgcg cttgatggtg ttgtagctgt cccaatacat
     4141 gcctccgttg atggcgcaac tgcacactgc caccacaaac ttggggccct gcatctgttc
     4201 ataactgcgc acgatccgct taagcgtctt gatggataca taaccgccta tcacaaacac
     4261 agatgcctgc ctgggagtgg ggcgcatctg catgcccaga cggctgatat cgtaacggga
     4321 ggtgaccaaa ggccgcagct cgatggcccc gcaacctgtc ccaaaagcca gaatccagag
     4381 gctgtgagcc ctgcaccagt tgaaaaactt ctccatgatc tggttgctgg tcctggctgg
     4441 ctgaggacgc gcgtccgcga accagtctcg ttcttcctct ggaagtcctt cgttgcggag
     4501 tttgcagtct tccgagcgcc attccgtatt gtggaatgga tagcgggaag ctggttcagt
     4561 cggatcggtt ccagcctccg cgggaatgac agactctggc ggaggagtct cttcctgaat
     4621 gtctagatct ttatcttcta tcatcataac tcctaaatag tccagatcac cactgctacg
     4681 atgccgatca gggtgggata cttgaggaag aagcggatgc tgtcttcagc cctgaacctt
     4741 gggaaggcgg cccccacaaa cacgctgaac atatagatcc cgaaagtctt ggccatcaaa
     4801 accgggatat tggtagcgcc gccaaagaag aggttcataa acagcaccag cttggcaccg
     4861 ttgaaaatgc cacggttagt ggccaacagc cccagataat tggcctgata ttcagtgggt
     4921 ggccccatcg ggatttcatt gggcgcgatc accagcgaga agggattatg catgttcatg
     4981 cccagcatgg caatcatggc ggcaatcaca gccaggggat tggtaaacag cgtccaggtg
     5041 aggaaaccac cctgttgggc ggctacgata tcgatgatat tgaggctccc atattggatg
     5101 gcaatactga tcacgctgag cgcgaagggt acttcaaaag cagtcatttg ggccaggcct
     5161 ctggccaccg cgatggcgga atagggatga cctccctctc ctgctccgag ggccatgccc
     5221 agctggccaa agaagataaa atacatcacc agcagcaggt ctccggacat gctgaaattg
     5281 ctgaagtaga tattgccaaa cagcaccggg ataaacatat aggtaccaat cccacccgcg
     5341 atgcgaaaca ccggtcccag gaagaacatc actccatggg tgatggcagt acgcttggcg
     5401 ttgtttttga tgatatcgat gaagggctgc cagacgggag gcccgagccg accttgcaca
     5461 cgggcaaaga tcttggccac gatgccggtg agggtgaggc cccacacggt gaccactccc
     5521 agagaaatga aagcccatac aagtcttaag gtcaggctca tttgatcacc cccagaatca
     5581 taaacattac tatggtatag agaaagatat gcaagaggta ggtctgcccg tttccggtat
     5641 agagcctgcc gaaagtggcc cccagagact gagaccactc tcccacaccg ttccaaaagc
     5701 tcactgccct cggtttcacc caactcccca aagccttctg gtaatgggca aacatgttgt
     5761 aagccacatg ggtggtccaa ggtttataag gacgctcggc ggcatagacg atgttgaact
     5821 gcttcacctt ctgaggcttg gcgatcacca gcatcagcca gatcagtggc accataaaca
     5881 ctcccatggt cacatacatc accagatttc cgttccagta ggcctgtaaa ttgggagatg
     5941 cgaggctgat gttgtaaccc gtccagctca accactccgg cttgggaatg aaatccgcga
     6001 tcgcctttga gatgggctgc gtgatcagat tgggaaacat ggaagcaccc atgatcagca
     6061 tgatgaagac tccctgaggg atcaggaacc agatgggagc ctctttcaca tgagcttgac
     6121 tgggtttggg ctgccccaga aataccgtgt gtataagttt atagagatag aggaaagcca
     6181 cggcactgga gaaaaaggcc accgcagtct gcagataacg tccctgctca atgagggccg
     6241 tgtaggtgaa ccacttggag ccaaaacccg tgaggggtgg cactcccgat accgcaatga
     6301 tgcccatcag cacactgata aaggagatcg gcatcttttt gatcaatccc cccatctcat
     6361 agagcttgcg cgtcccggtt ctatagatca ctcccgctat cgccatgaag atcaaggctt
     6421 tgaacatcat gtgggtgagg gccagataaa gagccgacat ccagcccagg tgcgtcatca
     6481 gagaaagacc cgtgactaca tagcccagct ggctcatgct ggaataggcc agcagcttct
     6541 tcacatcttc ctggaagaca gccattaggg cacctcccag agcggtaaac acgccaatcc
     6601 aacccaacgc gatgggtaaa acccctgttc cgatgctctt gacgctcatg cccagcacca
     6661 gcaccattcc aaagacaccc gccttggaaa gcaccgatga gatcaaagcg gtggagtcat
     6721 cctccgcttc agcataagct cccggcagcc aaacatgcac tcccagtgct cccattttga
     6781 tcaggaaccc tgccagcatc agcaccagag gcgtgaagcc gatctgcaca aagctgagct
     6841 gccagatgag cggataagtg agcgggaatt ccggcatcag catcagccct cccatgatca
     6901 ggaaagccgc gcccagcgag aaagtgatat agctgagacc cgcggccctg gcatggcggc
     6961 ctctcatgat cagcatccag gaggagacgg tcataaactc ccaggccagg aaaaactcca
     7021 gcttggagcc agccgtcaac aggttggcca gggagagaat cagcatcatc aacacaggaa
     7081 agaggccttc cgcctttccc caacggtgca tgaagccaaa gatctgcacg atcgccccac
     7141 ccaccagaat cacggcaaag agttggccca tgggtggcaa aacaggccag acctgcaaat
     7201 atccataagc ccccaaaccc aggatggcca tcaagccctt gagcctggca ggaaagaaat
     7261 ccaggaggct aaagaccagc agacccaata caggatagag agaattgacc ggcagcttga
     7321 gcaaaccggt ggggataaga taggagacca caaacagggt gagcactccc agcaccacag
     7381 gcatatagcg atgcgcgcca ggacgatagc cctcggggct gactcctttg gcagcaaagc
     7441 ccagccatcg gaacatatag aaggcttcca acagcgagcc gatcaggatg ggaatcacca
     7501 gatagggttg atcgattgta aatagcaccc gcaccagttc ccacttggca aagaagcccg
     7561 ggaagggtgg caaccccacc agcgctatcg tgaagatacc aaagcagatc agcagtccgc
     7621 tgtccgcttt gagcgcgctc caggacttca gagacttgcc ttccaccatc tcggaaagcc
     7681 agaacaggcc tcccttggcg aggctgttgg aaatgatgaa accgatggcg atcacgaggc
     7741 tgtaataacc cggcactcca agattgctga gcccgtagat aatgaagatt agcaggccgg
     7801 tctgagctgt ggaagaatac cccagcaggc gtttggcgtc cgtttgcctc agcgcgatca
     7861 gattggccat cacgaagctc accagacccg ctccggcaaa gagggccagc atgccataat
     7921 ccatcagcgg caacagctta tacatcagga agatcagcgc cgtgctatgc accgaggaga
     7981 ggatagctcc catgccgcca tgcgcgcttt gatagacgtc cagggcccaa ccgttggcag
     8041 ggaaaggctt tagatcgata aagacggcca tcgcgatcag gaacagcgcc acccggtagg
     8101 ccagagactc agccacggga ttgaggatcc acatatccag attgagagtg cctgtaaagc
     8161 ggtaggcaaa gatgatgccg atcaggagca gcgcgctgct gatccctccc gccatcatat
     8221 acttgatccc cgaggccagg gacgtcctgg tttgtctgat cccgatcagc gcgtaggatg
     8281 cgatggagag gatttccatg aatacaaaag cgttgaagat atcccgcgtg agaatcaatc
     8341 cactagcccc cagcagcaac agcatataca atgccatggg ctgggctcca tccagaaaga
     8401 acctgcgccc aaaggctatc gaacccagga atcccattac caggaccacg atcagcagga
     8461 tgacttcctg aaagccaaaa ctgaggccga tggagagcgg gacctcaaag cccgccgtga
     8521 aaaactggct ctgcagggct ggattctggc taagctccca aagccggaat cccaccagga
     8581 ccaatgcggc ggccagagtg gcgtagagca ggcccaatcc tatctcacgc ttcactttat
     8641 caaagagcgc gatggcaaac gccgaggcca gaaacagggc gatcaaataa aggggattta
     8701 ccatttttgc tcctcgtggt cgtcgatatt ggtgctgccc gtggctttat tgagccgcac
     8761 cgcgtaagcc agcatcagtc cagtcacggc caggccgatc acgatcgccg tgagcaccag
     8821 ggcagacgga acggggtcca cgaccttgct gatggcctgc attcctgcaa aagatgcatc
     8881 ggcggtcgaa acggctctgt cgataatcgg ggccgtcccg cctttcacat agccgatggc
     8941 cacgatcaga agatgcacgc ccgtgtccac aacggtgaac ccgatgatca tcctgatgat
     9001 gtctttcttg accaggatgg cccagattcc agccaggatc aacagcattc cggtgatgag
     9061 cagaatcata gatcctcccc ttcgtgctgg atggaggcca gcacactggc cagttcgctg
     9121 cccaccttga tacctatcaa tgaatagatg accggcacgg cacctgctga aaagagggca
     9181 agaaacttcc cggccagcaa atagcgggga tccaggaagt gatgtactcc caacaggata
     9241 agccctgcca gccccattgc cacataggct aggccagaca gcgactcact gatatgcagg
     9301 agcagatgag ataggtgaaa acggacctca gccatgatca acatcaggat gccggaagcg
     9361 atcacgactc cgccctggaa acctccccca gggctcaaat ggccgtgggt gaagatatag
     9421 atgcccaaca ccacgatcat cggcaccagc aggttcgatc ccgtcttgag aatctcggag
     9481 ccttccgtgc gtgttttaac tgctgagcta ctgagggnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnngag ttactgagat tgttctgggg atctgtcgtt cccgtagcat
     9661 cgaattccaa tccggcgagt gctgattcat cgcaaacggg gacgtttgcg gctccatcag
     9721 catcgtcaat ctccaatcca gcgcttcttt tctttctttt caacaaaaac cctatccccg
     9781 ccgtggccaa aaacagcacc gtcacctctc ccagggtgtc cagtccgcgg tagctcacca
     9841 ccacagcggt caccgcgttg ggcatattga gatcactggt ggagcctcgc acatattccg
     9901 aggccagagg gttaagctct tgcgcggttt ggaattccac tcccagttgc cagaggatcc
     9961 agcccatgcc aaggatggcc gcaaagacca ataaatactt aatcatcttt gccctccctg
    10021 ctggcgcgga tatgcttgag ggcatagagg aagatcaccg tggtaagtcc cgctccgata
    10081 gttgcttccg tcatcgccac atccggcgcg gcagccagca gaaacattat ggacgcggaa
    10141 aggctgatca ctcccgacgc gatcacggcg ctcaccactc ttttggtata gatggcaaag
    10201 atggccgccg ctacgctaat caggcccaaa aagatcaagg taatggtttc aaacattgca
    10261 ttcctccccg tcctcaaagg cctcatcgcc ccgctcggca agctcgtccc tcacgctgcg
    10321 gtccgtgagc ttgatgccca ggcggtgcga agcccggctc atggcatgag aactgatggg
    10381 attggtgaaa aggatgaaca gcaccagtaa aatgaggcga ggcagccagg cgggcaggat
    10441 caatccgatc ccgatcaata ccaggatcga acccagcgaa gtggctttgg tgcctgcctg
    10501 catacggtta tagacgtccg gcatcctcaa tactcccagt ccgcccagga aaagcagaat
    10561 agagcccaga agagtgacaa ttgctcccgc gatctgcatc agagccccct ttccatatag
    10621 cgcgccacca cgatcccacc cagaaaactg agcaggccat agaccagcgc cacatccaaa
    10681 tagatgatcc tgccagcatg gagggccagc atggcaatca cagagatgcc caacacagtc
    10741 ataacgtcca gcgccacaac ccgatccgcg caggtgggcc ccttcaaaaa acggataaag
    10801 gccagcagca tcccggccag catgatgtaa aacgagctct gcatcagcag cataacggtg
    10861 ctatccaaag atcacctcca gatatttctc aaaggtgctc acaatcttgc gggtggcatc
    10921 gtccacatcg ctggactgaa tatcgatcca gtggatgtag aggtcttccc catccgtttc
    10981 caccgtcatc gttccagggg tgagcgtgat cgaattggcc agcacgatgc gccccagagg
    11041 gcttttcagc ctggtcttca ctttcacgat gccagggttg atcggcaggc tgggactcac
    11101 cacccggaag gccacatcca gggtgctctt catcaattca cgcagaaaca ccaggatata
    11161 gacggggatg taaatcaagg agaggggtgt gagcctcaga tctcccagct gggaaatccg
    11221 cggtagaaaa atcaaggaaa tcagcggtgc cacaatggca gccacgatca gctcatccct
    11281 cgcagtgcct cccaacgcga tccaaagcgc gaacagcagc acagtgctga ggacaaaggc
    11341 tttgagcttg tgcataaaac ctcgcttata tcaactattg catggggtga aattctgtac
    11401 ttaaatagac ttgtttacaa gtttggccat accatcaaat ctgtcaagag agaattgtct
    11461 ttcagaccaa aatctgctta ataagaactt taaccactta tgccatgtcg ctgttcagcg
    11521 gccaggatca ctcggagagc tatcattgct tggcacataa aaaatgagcg acaggtgtaa
    11581 acaacctcac actataagaa gccgatgcac atggtgtatc actggcttca aagcttgcgc
    11641 aagaactggc attttgagtt ccgtaggaac ggtagtttag atagatacgc gtgctcctat
    11701 caataatggg agtcccatgg ggacggcagt ttagattaga ctattacgat gataatgagt
    11761 tat
//
LOCUS       DAOSFN010000074        11735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4615_length_11735_cov_8.682962, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000074 DAOSFN010000000
VERSION     DAOSFN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11735
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4615_length_11735_cov_8.682962"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PLO35_05980"
     CDS             complement(<1..1127)
                     /locus_tag="PLO35_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOH79252.1"
                     /translation="MTLHQLLKALDFERLAGTDGEARGRTVISDYLKRLGLEPQVEAF
                     EIHGFDTGTATVSGPSSSWEATPYGLCGDTDVEGELVYLENADALMYNPGAYDDKIVL
                     YYHSDKRLYDLCEYSRVKAFIGISAPHKKAYSYSHRQNRERQEIFPAVMIRYDYAEKL
                     MKLAGQQVKLSIRQTAQTKTAHNIVLDIPGTDPDQNLTLLVGHYDSVARSHGSSDNAG
                     GSVCLIKTAQYFAAHPPKRDLRIIWFSAEELGLLGSFEYVRAHQEEIVQRVKLVINVD
                     LAGDPIGRNVMFVLGSKELMGYAGGVLREAGLLFSENLSIYSSDCMPFCVHEIPSINL
                     ARVGGKALFYIHTEDDVAKHTSEYGLRDVYEATRTLLKRVL"
     gene            1357..2100
                     /locus_tag="PLO35_05985"
     CDS             1357..2100
                     /locus_tag="PLO35_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOH79253.1"
                     /translation="MPQIRDNVLAVTAAIKNKLSALGRDPEDAILVAVTKTHPVEAIE
                     AALEAGLGHFGENKVQEAQRKVPFITRPYEGFHFIGHLQTNKIKALLELKPCLIHSID
                     SLYLAESLNAALARFNRVQDILVQVNTTQEASKSGVDLSNAIEMVGQISQLPFLRVKG
                     LMTIGLMSIDAERTRPHFQKLKQVFDQINTMIEEGSFASGDPGSSAVEMRWLSMGMSD
                     DFLVALEEGSNMLRLGSVLFGARNYGSGK"
     gene            2097..2741
                     /locus_tag="PLO35_05990"
     CDS             2097..2741
                     /locus_tag="PLO35_05990"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOH79254.1"
                     /translation="MIHLLALGIVIIAYMYGCFSTARIIAKSFRSLDIYKVGTGLADT
                     ENIYSHISRPLGVLVGGLDVAKAYLFMKVVELLLIMLSGSDLMTGVEMIYAPNYMLVY
                     GLAMLVGHCLPVTHRWKGGRGIFTYTGFMVYFAFWPMIITLVIAWLLIAIYKQIRFAQ
                     YLIVILPVILAQVFYSFIPYFRKELPPYFIGMMLGVALFMGILNFVVSKKLGEL"
     gene            2769..3887
                     /locus_tag="PLO35_05995"
     CDS             2769..3887
                     /locus_tag="PLO35_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH79255.1"
                     /translation="MLQIKEVKSKKDVKAFVMLPFRLYEGDPNWVPPLIKDQIKFLNP
                     LHNPFFEHSEASLYLAIRDGRVVGRISAHTNTRHNLEHKDEVGFFGFFECEEDPEAAK
                     ALLNTALQWNRARNRSAVRGPLNFTINDECGMLVDGFDSPPMIMNRHDKPYYQHLLEG
                     CGFVKAMDMYAYLSIRTEMPERIDKVAALIEKRAGVKIRPLSRNKAARAKDIETIFHI
                     YSKAWEYNWGNVPMTQAELKHLASELLPLADPDMIFIAEIEGQPVGFSLAMPNYNEVL
                     KVMNGRTHLWALIKAMIAKRRIGSARVVTMGLIEEYRKRGIDTLFYYYSYKNGLPKGY
                     IRGEFSWVLENNTMMNRVAEMLDAQRYKTYRIYEHPID"
     gene            3868..4788
                     /locus_tag="PLO35_06000"
     CDS             3868..4788
                     /locus_tag="PLO35_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH79256.1"
                     /translation="MNTPSTELSLRRNWLSPIDIITLVFTGWIMLYMGIGHPRVIDLG
                     THFPSYMSVFAGVLLTAWLQKLWEGREGWFYSAFRLLRSMYPVLLFGYFYSSNYAFNR
                     VLVPNWQDPFFYKIDEAIFGYLPSLVWGQKYSHWAVSELFHLAYFCYYPMIFGLPLYL
                     YLKNRNAFAELIFAVTFTFYLSYFIYSLLPTIGGRYFPEALELTKVYRAGPFTHIMAY
                     IYNTTHHWGGAFPSSHVGVTIVLTIAGLRFTKVMGYVFVVISFFLALSIAYCHYHWFI
                     DAVGGIFLGIGGYYLAGWLRTKLSPVENSN"
     gene            4788..5840
                     /locus_tag="PLO35_06005"
     CDS             4788..5840
                     /locus_tag="PLO35_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79257.1"
                     /translation="MKRSILIILAVGIASLAAQQAALPITQYDYAIPENNVSPIAIGM
                     GGLNLTYSGDPYCSYSNPALLAEVEQSSLVTSFRLANDEEMSILEATSLSNTLKDKQF
                     KYFSLSTKKAAFTYQPMSRVNISEIIAENGVNYNLYHDFMLDKVQLSIAAKDEERSAL
                     SAGLNLKYLSGRLVYLKERRIGSTTFTREAFIDDKVRGFSTDLGFTLDRETFKFGLTA
                     YDLFSRLYWENYSSKPIQSRIGLGAAWVDDNMLISMGLQGKISKSTDTTYHLGFQNSW
                     SWGQNYNQAGRPIQHSILLRLGLYSQDFYGTKNIHFTMGSGYNYSIFRFDFSVNSAGM
                     ILKESEYLFSLGVGLP"
     gene            5844..7841
                     /locus_tag="PLO35_06010"
     CDS             5844..7841
                     /locus_tag="PLO35_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HOH79258.1"
                     /translation="MFQLGFLHSGLLIFAAATVLPLIIWLIARRKPPRVIFSSLRFIK
                     LSQEQEKKRSKLKNILLLIIRMLIILLIALAVARPQVYSSRLSKSSKHPPTALAIVLD
                     TSFSMDYLHESRTLLDRAKEALSRINGLCGPSDKISLITLDESWNTLHSQIFAGSIPA
                     DLISALRVTHTPIRMEEALQMARGRLIESGLPNRELYLITDGQQNQYPEDLDIPAYLI
                     PLDTPKSVANLSVKDVQPRPQLVDKTRLQSLDFTLINHGPTDRKDVLIRAILGDTKVA
                     EKFVTINARQEIRESILVELREDGWQTGYVEVVDEGLLHDNRSWFAFPHRVAPSVAVI
                     SQKPSLPFYLDSALRVYTGSAGSLKVMDPATLTMADLDRYQNFVIYAAGNPNARLKEL
                     IDALIKAERGALVLLDEGLSPEAKSMWESTFGCRIGSFIQQPKDIASLNPHHYISALL
                     SGKDMAQKRISHYWSCRAGNSASLASAGGDDLVMVKGRMVLFAFDPAALSNSFFIGPI
                     FPVLTYRALEHSGLAQASGSRIKLGDLITADTLVLPDGSSLELARRAYRANFPGIYQS
                     KVGSFDPIAIAVDHDPAESDITPHDFSRLKYVKPLKSNWSEQVFRSRLGHDIWKILLM
                     IALALFLLEIVIVKLSQSSSGLPGDQAIPQPGSGLNQPSKE"
     gene            7844..9706
                     /gene="dxs"
                     /locus_tag="PLO35_06015"
     CDS             7844..9706
                     /gene="dxs"
                     /locus_tag="PLO35_06015"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOH79259.1"
                     /translation="MLQDIKDPAQIRKLTMAEMVLLAKETRQRIVDVVSKTGGHIAPS
                     LGAVEIAIALLNIFDFNLDRVVWDVGHQSYAWKILTGRNERFETLRVLDGISGFTNRD
                     ESPYDAFSTGHSSTSISAALGLSCASRLLQEKKAHHIAVIGDGALTGGMSFEALNHAG
                     HLQPDRFIVILNDNAMSISKNVGGLQKYMARMMASRSYNTLKQQIWELSNSLPDNIRR
                     RFIYGAQKLEESMMNILVPNIIFEDLGFKYVGPIDGHDLKQLTSIFKRVRDNMVGPVL
                     LHVVTQKGKGYSPAEKNASLFHGVGPFESSSGKQVCSGKESWSEVFGNSLLRLAERQP
                     NIVAVTAAMSAGTGLSAFEARFPDRFFDVGIAEQHAVTLAAGMACKGIKPFVAIYSTF
                     MQRALDQVIHDVALPRLPVVFCIDRAGLVGEDGATHHGAFDLSFLSGVPGLIILAPSS
                     AEDLDAMLAWAATYQKGPVAIRYPRGTAFCSDAGIDEFMPGMARLVDDNGQIALVGVG
                     DAFHLAADVHKLLQDEGIPTRLVDLRSVKPMDEILLRRLGDECAYIFSCETGSIIGGA
                     GARIAQLLWDKPSKVINFGYPDSFVPHGKTELLKQRIGFTPEALAERIIQITQT"
     gene            9703..11064
                     /gene="mnmE"
                     /locus_tag="PLO35_06020"
     CDS             9703..11064
                     /gene="mnmE"
                     /locus_tag="PLO35_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOH79260.1"
                     /translation="MRLDLKEPICALITPAGTAAINVIRISGEGSIGIVAAHFDSPHK
                     LLKAKGKQIVYGVFLDADKQPLDEVLCSIFRAPHSYTGDDTVEISCHGNPRIAARILE
                     LLLSSARLANPGEFTLRAMHNNKIDLIQAEAINDLISAPTSRSGSAALSQTRGVLSNS
                     LKSILERITHARLRCEMAIDFADQDLPQIDTEALINDLARLDSEVSQLCEQGSQGRYI
                     REGIRICLAGAPNSGKSSLFNAFLKHNRAIVTPHPGTTRDYLEETLSLRGYAIILYDT
                     AGLRDTSDEVEQQGIDRSRELMFDADLVLFLIDASRLAEHKEDDIPQGLGNKIQIVIS
                     KADLLEKDDLEGLLGTGSIPASAISPDGLDELSEAILERFQLAEPLPERPLVTNTRHL
                     AALRSCGEGLQNALVALRSGLGFEFAAFDLISASAALEQILGVITPDDLLERIFADFC
                     IGK"
     gene            11080..>11735
                     /locus_tag="PLO35_06025"
     CDS             11080..>11735
                     /locus_tag="PLO35_06025"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HOH79261.1"
                     /translation="MSNWIYSKNGKEYGPMSTSKVADAILRGELELDDYVLSAETQLW
                     KKAKDVQAIMDIVHKPLPNNLFDDVNREEFSSFVKGDESVYAEPIFFNMPYTRLMILQ
                     VLTLGFFQTYWFIRQYMYLIRRPGRKPMRISLMVFAFAFYIFNEVEHDRELNSAYRAP
                     WSSWRMAILWYLVIPLATFGLQTNNPILEIVLSLPVVVFGTSLVLIPVQKYINECNEK
                     L"
BASE COUNT         2725 a         3128 c         3160 g         2722 t
ORIGIN      
        1 ttgaggacgc gtttcaggag ggtccgggtg gcctcataga cgtcgcggag gccgtattcg
       61 ctggtgtgtt tggccacgtc gtcctcggtg tggatgtaga agagggcctt gccacccacg
      121 cgggccaggt ttatagaagg gatctcatgc acgcagaaag gcatgcagtc gctgctgtag
      181 atgctgaggt tttcgctgaa gagcagaccc gcttccctga gcactccacc cgcgtaaccc
      241 atcagctctt tggagcccag aacaaacatc acgttgcggc cgatggggtc tccggccagg
      301 tccacattga tcaccagctt cactctctgg acaatctctt cctgatgggc cctcacatat
      361 tcaaagctgc ccagcaggcc aagttcttcg gccgaaaacc agatgattct gaggtcgcgt
      421 ttgggagggt gggcggcaaa atactgggcg gttttgatca ggcaaacgct tccgcccgcg
      481 ttgtctgaag aaccgtgcga cctggccacg ctgtcgtaat ggcccaccag cagggtgaga
      541 ttctggtcgg gatcggtgcc agggatgtcc agaacgatgt tgtgagcggt tttggtctgg
      601 gcggtctggc ggatgctgag tttgacctgc tgtccggcca gtttcatcaa tttctcggca
      661 tagtcgtagc ggatcatcac cgccggaaaa atttcctggc gttcacgatt ctggcggtgg
      721 gaatatgaat aggctttttt atgaggggcg gaaatgccga taaaggcttt gacgcgagag
      781 tattcgcaaa ggtcatacaa acgcttgtcg ctgtgataat aaagcacgat tttatcgtca
      841 taggcgccgg ggttatacat gagggcgtcg gcgttttcca gatagaccag ttcgccttcc
      901 acatcagtat cgccgcagag gccataggga gtggcttccc agctggaaga cgggccactc
      961 acggtggcgg tcccggtgtc aaagccatgt atttcaaaag cttccacctg gggttccaga
     1021 cccagccgct tgagataatc gctgatcaca gttctgcctc tggcctctcc gtccgtgccg
     1081 gcaaggcgtt caaaatccag tgctttgagc agttgatgta aggtcataat cgcgctcctg
     1141 atctagtttt ttagtttcgg tcactctacg cccaaacggg tttggctgtc aagctaaaag
     1201 gcgtggatga tattctgctt gacaggctgg gggcttggaa cagattggac aaatccctct
     1261 aatgatacag gtgtatctgc ctggggatat ggcgccaggt gtccaactgc ttaacagcca
     1321 gagacataga cgccgcacaa aagattagga gtattgatgc cccagatccg ggacaatgta
     1381 ttggcggtca cagccgcaat aaagaataaa ctctcagcct tgggccgcga cccggaagac
     1441 gctatcctgg ttgcggttac gaagacccat ccggtggagg cgatcgaagc cgctctggag
     1501 gcggggctag gacattttgg cgaaaacaag gtgcaggaag cccagcgcaa agtgcccttc
     1561 atcaccaggc catatgaggg ttttcacttt atcggccacc tccagaccaa caagatcaaa
     1621 gcgctgctgg agctcaaacc ctgcctcatc cacagcatcg attcactcta tctggccgaa
     1681 agcctcaacg ccgctctggc cagattcaat cgggtgcaag acatcctcgt gcaggtcaac
     1741 actactcagg aagcctccaa gagcggagtg gacctaagta acgccataga gatggtgggg
     1801 caaatctccc agcttccgtt tctgagggtt aaaggcctga tgacgatagg tttgatgagt
     1861 atcgacgcgg agcggacccg tccccacttt cagaaactga agcaggtgtt tgatcagata
     1921 aatactatga ttgaagaagg ctcattcgcc agcggggacc ctggcagttc cgctgtcgaa
     1981 atgcgctggc tctcaatggg catgagcgac gattttctgg tggccctgga agagggttcc
     2041 aacatgttgc gcctgggatc cgtgctgttc ggggcacgta actatgggag tggcaaatga
     2101 ttcacctgct ggccttgggg atcgtgatca tagcttatat gtacggttgt ttttccacgg
     2161 cccgcattat tgccaagagc tttcgttctc tggatattta taaggtgggg actggcctgg
     2221 cggatactga aaacatctat tcccatatca gcagaccttt gggagtgttg gtggggggac
     2281 tggacgtggc caaagcctat ctgtttatga aggttgtgga gctgctgctg attatgttgt
     2341 cgggcagcga tctgatgacc ggagtggaga tgatctacgc gcccaactac atgctggtct
     2401 atggacttgc catgctggtg ggacactgtc tgcccgtcac acatcgctgg aaggggggcc
     2461 ggggcatctt tacctatacg ggattcatgg tctatttcgc gttctggccg atgataatca
     2521 ctctcgtcat cgcctggttg ctgatcgcca tctacaaaca gatccgcttc gcgcagtatt
     2581 tgatcgtgat cctgcctgtg atcctggcgc aggtgttcta ttcctttatc ccctatttcc
     2641 gcaaagaact gccgccatat tttatcggca tgatgctggg agtagccctg tttatgggta
     2701 tcctcaactt tgtggtgtcc aagaaactgg gcgagctgtg atcctgaatt aaccccaagg
     2761 atattgatat gttgcagatc aaggaagtga agagcaagaa ggatgtcaaa gcctttgtca
     2821 tgctgccttt ccggctctac gaaggagacc ccaactgggt gccgcctctc atcaaagacc
     2881 agatcaagtt tctaaacccc ctccacaatc ctttttttga gcactccgag gccagtctgt
     2941 atctggccat ccgggacggc agagtagtgg gaaggatttc cgctcacacc aatacccgtc
     3001 ataacctgga gcataaagac gaagtgggtt tctttggatt ctttgagtgt gaagaagatc
     3061 ccgaagcagc caaagcgttg ttaaataccg cgctgcaatg gaatcgggcc agaaaccgca
     3121 gcgccgtgag aggtcccctc aattttacca tcaacgacga atgcggtatg ctggtggatg
     3181 gtttcgacag ccctcccatg atcatgaacc gtcacgataa accctactac cagcatctgt
     3241 tagagggttg tggcttcgtg aaggcgatgg acatgtatgc ttatttgagc atccgcaccg
     3301 agatgcctga aaggatcgac aaagtggcgg ccctgatcga aaaacgcgcg ggcgtcaaaa
     3361 tccgcccgct cagtcgtaac aaagcggcgc gggccaaaga tatagaaact atctttcata
     3421 tctattccaa agcctgggag tataactggg gcaacgttcc catgacccag gcagagctca
     3481 aacacttggc atcagagctt ttaccgctgg ccgatccgga catgattttc atcgccgaga
     3541 tcgaaggcca gccggtgggt ttcagcctcg cgatgcccaa ttacaacgaa gtcctcaaag
     3601 tgatgaatgg ccgcactcat ctgtgggccc tgatcaaagc tatgatcgcc aaaaggcgga
     3661 ttggctccgc ccgggtggtg accatgggtc tgatagagga gtatcgtaaa cgcggcatag
     3721 acacgctatt ttattactat tcatataaaa acggcctccc caaaggctat atccggggcg
     3781 agttctcctg ggtgctggaa aataacacga tgatgaacag agtggctgaa atgctggacg
     3841 cgcaaagata taagacctac agaatctatg aacaccccat cgactgagct atccttgcgg
     3901 agaaactggc tgagtcccat cgatatcatc acgcttgttt tcacaggctg gatcatgctc
     3961 tacatgggga ttggccaccc gcgggtgatc gatctgggca cccatttccc cagctacatg
     4021 tctgtgttcg cgggagtgtt gctgacggcc tggctgcaaa agctctggga gggcagggaa
     4081 ggctggtttt acagcgcttt ccggctgctg aggagcatgt atccggtgct gctcttcggt
     4141 tatttttact cttccaacta tgctttcaac cgcgtgctgg tccccaattg gcaggatccc
     4201 ttcttctata agatcgatga ggctatcttt ggatatctgc cttcgctggt ctgggggcaa
     4261 aagtattccc actgggcggt ttcggagctc ttccacctgg cctatttctg ttattatccc
     4321 atgattttcg gcctgccgct ctatctctat ctgaaaaacc gcaacgcctt cgcggagttg
     4381 atcttcgcgg tgaccttcac cttctatctc agctatttca tttattccct gctgcccacc
     4441 attggaggca ggtattttcc tgaggcactg gagcttacca aggtgtatcg ggctgggccc
     4501 ttcacccaca tcatggccta tatctacaac actacgcatc actggggcgg cgcttttccc
     4561 agttctcatg tgggggtcac gatcgtactc accatcgcgg ggctgagatt taccaaggtg
     4621 atgggctatg tgtttgtggt gatatccttt ttcttggcgc tgtcaatcgc ctactgccat
     4681 tatcactggt ttatagatgc cgtcggaggt atctttttgg ggatcggagg ctactatttg
     4741 gccggctggt tgagaacaaa gctatcgccg gtggagaaca gcaattaatg aaaagatcga
     4801 tattgataat cctggcggtg gggatcgcaa gtctggcggc ccagcaagct gctctgccga
     4861 tcactcaata tgactacgcc attccggaga acaacgtctc tccgatcgcg attggcatgg
     4921 gcggcttgaa tctcacttat tccggagatc cctattgcag ctattccaat cccgccctgc
     4981 tggcagaagt ggagcagagc agcctggtaa cctccttccg gctggccaat gatgaagaga
     5041 tgagcattct ggaagccacc tcgctttcca ataccctcaa ggataagcaa ttcaaatact
     5101 tcagcctcag caccaaaaag gccgccttca cctatcagcc catgagccgg gtgaatatca
     5161 gcgagatcat tgccgaaaac ggagttaact acaacctcta tcatgacttt atgctggaca
     5221 aggtgcagct atccattgcc gccaaagatg aagaacgctc cgccctgagc gcgggcctca
     5281 acctcaagta tttgagcgga cgtctggtgt atctgaaaga acggcgcata ggctctacca
     5341 cctttaccag agaagctttt atcgatgaca aagtgagggg tttttccacc gatctgggct
     5401 tcactctgga ccgcgagact ttcaagtttg ggctcacggc ttacgacctc ttctcacgcc
     5461 tctactggga aaactattcc tccaaaccca tccagtccag gattgggctc ggagccgcgt
     5521 gggtggacga taacatgctg atcagcatgg gtttacaggg aaagatcagc aaaagcaccg
     5581 acaccactta tcatctgggc tttcaaaaca gctggagctg gggccagaac tacaatcagg
     5641 ctggccgtcc cattcagcac tccatcctct tgaggctggg gctttacagc caggattttt
     5701 atggcaccaa aaacatccat ttcacgatgg gatcaggcta taactacagc attttccgct
     5761 ttgacttttc cgtgaacagc gccgggatga tacttaaaga gagcgagtat ctcttttctc
     5821 tgggggtggg gctcccctaa gctatgttcc agcttggatt tctgcacagc ggcctgctga
     5881 ttttcgcggc ggccactgtc ctgcccttga tcatctggct gatcgccaga cgcaaaccgc
     5941 cccgggtgat cttcagttca ctgcggttta tcaagctcag ccaggagcag gaaaagaagc
     6001 gcagcaagct caaaaacata ctgctcttga tcatccgcat gctgatcatc ctcctgatcg
     6061 ctctggccgt ggccaggcct caagtgtatt ccagccggct gagtaagagc tccaaacatc
     6121 ctcccaccgc tttggcaatc gtgctggaca cttctttcag catggactat ctgcatgaat
     6181 ccaggaccct gttggacaga gcgaaagagg cactttccag gattaacggc ctctgtggtc
     6241 cctctgataa aatctcgttg atcacccttg acgagagctg gaacaccttg cattcacaga
     6301 tcttcgcggg gtccattcca gctgatttga tctcagccct ccgcgtcacc catactccca
     6361 tcagaatgga ggaggccctg caaatggcca ggggccgtct gatcgaatcc gggttgccca
     6421 atcgcgagct ctatctgatc acagacggcc agcaaaacca gtatcctgaa gatctggata
     6481 tcccggccta tctgatccct ctggatactc caaaaagcgt tgccaacctt agcgttaaag
     6541 acgttcagcc caggcctcag ctagtggata agacccgcct gcagagcctg gattttaccc
     6601 tgataaacca tggccccacc gatcgtaagg atgtgttgat cagagcgata cttggcgata
     6661 ccaaggtggc cgagaagttt gtgaccatca acgcccgtca ggaaatcaga gaaagcatcc
     6721 tggtggaact gcgtgaagat ggctggcaga caggctatgt ggaagtagtg gatgaagggc
     6781 tcctgcacga caaccgctcc tggtttgctt ttcctcatcg tgtggccccc agcgtggctg
     6841 tgatcagcca gaaaccctct ctaccatttt atctggacag cgcgctaaga gtctataccg
     6901 gtagtgccgg aagcttgaaa gtgatggacc ccgccacttt gaccatggcc gatctggata
     6961 gatatcagaa tttcgtgatc tacgcagccg gaaaccccaa cgctcgtctt aaagaactga
     7021 tagacgcgct cataaaggct gaaaggggag ccctggtgct tctggacgaa ggcctgagcc
     7081 cggaggctaa aagcatgtgg gagtccacct tcggctgcag gataggtagt tttatccagc
     7141 agcccaaaga catcgccagc ctcaatccgc atcactatat cagtgccttg ctcagcggta
     7201 aagacatggc ccagaagcgg ataagccatt actggagttg ccgggcgggg aactctgcca
     7261 gccttgcttc agccggtgga gacgatctgg tgatggtgaa aggcagaatg gtgctgttcg
     7321 cgtttgatcc cgcggccctc tccaatagct ttttcatcgg tcccatcttt ccagtgctga
     7381 cttacagagc actggagcac agtggcttgg cccaggccag cggatcgcgg atcaagctgg
     7441 gagatctgat cacggcggac actctggtct tgccggacgg ttcttcgcta gaactggcca
     7501 gacgtgccta tcgggccaac tttccgggta tctatcaaag caaagtgggt agtttcgatc
     7561 ccatcgccat cgcggtggac cacgatcccg ctgaaagtga tataacccca cacgatttca
     7621 gccggctgaa atacgttaaa cctctcaaaa gcaattggtc agaacaggtc ttccgctcca
     7681 gattgggaca cgatatctgg aagatcctct tgatgatagc tttggccctt ttcctgctgg
     7741 agatcgtgat cgtcaagctc agtcagtcca gttcaggtct gcccggagac caggcaattc
     7801 cgcaacccgg cagcggttta aaccagcctt ccaaggagta atcatgctgc aagatatcaa
     7861 agaccccgct cagatcagaa agctgaccat ggcggagatg gtgctgctgg ccaaagaaac
     7921 ccggcagcgc atcgtggatg tggtttccaa aactggcggg cacatagccc ccagcctggg
     7981 tgccgtggaa attgccatcg cgcttttgaa tatcttcgat ttcaatctcg accgggtggt
     8041 ctgggatgtg ggacatcaaa gctatgcctg gaagatactt actggccgca atgagcgctt
     8101 tgagaccctc agggtcctgg atggcatcag cgggtttacc aaccgggatg aaagtcccta
     8161 tgatgccttc agcactggtc acagcagcac ttccatttcc gcggcgctgg gtctgtcctg
     8221 tgcctccaga ctgctgcagg agaaaaaggc gcatcacatt gccgtgatag gagatggagc
     8281 gctcacaggg ggcatgagct ttgaggctct caaccatgcc ggtcacctgc agccggaccg
     8341 ttttatcgtg atcctcaacg acaacgccat gtccatctcc aaaaacgtgg gcggcctcca
     8401 gaaatacatg gccaggatga tggcctcgcg tagctacaac accctcaaac aacagatctg
     8461 ggagctcagc aacagcctgc cggacaacat tcgccgccgc ttcatctatg gcgctcaaaa
     8521 gctggaagaa tcgatgatga acatcctggt gcccaacatc atcttcgaag atctcggctt
     8581 caaatatgta gggcccatcg atggtcatga cctcaaacag ctcacctcca tcttcaaacg
     8641 agtgcgggat aacatggtcg gaccggtact gctgcacgtc gtcacccaaa aaggcaaggg
     8701 ttacagtcca gccgaaaaga acgcctctct ctttcatggc gtggggccct ttgaaagcag
     8761 cagcggcaag caggtctgca gcggcaagga gagctggagt gaagtgtttg gcaacagctt
     8821 gctcagattg gctgagcgcc agccaaacat cgtagccgtc acggccgcca tgagtgcggg
     8881 gaccgggctg agcgcttttg aagccagatt tccggatcgc tttttcgatg tcggcatagc
     8941 cgaacagcat gccgtcaccc tggcggcagg catggcctgt aaagggatca agcccttcgt
     9001 ggccatctac agcactttta tgcagagggc tttggaccag gtgatccacg atgtggcgct
     9061 gccacgtctg ccagtggtgt tttgtatcga cagagctggc ctcgtgggag aagacggtgc
     9121 cacacatcac ggtgctttcg atctcagctt cctgagcgga gtgcctggac tcataatcct
     9181 ggccccctcc agcgctgaag acctggacgc catgctggcc tgggccgcga cctatcagaa
     9241 aggtcccgtg gcgatccgtt atcccagagg gacagcgttc tgttcagacg ctggaattga
     9301 tgaattcatg ccagggatgg ccaggcttgt agatgataat ggccagatag ctttggtggg
     9361 tgtgggtgac gcgtttcatc tcgccgcgga tgtccacaaa ctcttgcagg atgaaggcat
     9421 cccaaccaga ctggtggacc tgcgcagcgt aaagcctatg gatgaaattc ttttacgtcg
     9481 attaggcgac gaatgtgctt acatcttcag ctgtgaaacg ggcagcatca taggtggggc
     9541 gggagccaga atagctcagc tgctctggga taaacccagc aaggtcatca acttcggata
     9601 tcctgacagt ttcgtgcccc acggcaagac ggaactgctc aaacaacgga ttggcttcac
     9661 gcccgaagcc ctggccgaaa ggatcatcca gatcacccaa acatgagact cgacctcaaa
     9721 gagcccatct gcgctttgat cactccagcc ggaacggctg ccatcaacgt gatcaggatc
     9781 agcggagagg gctcgatcgg gatcgtggcc gcccattttg attcccccca taaactgctg
     9841 aaggccaaag ggaagcagat cgtttatggc gtctttctgg atgcggacaa acagccgctg
     9901 gacgaggtcc tctgcagtat cttcagagcc cctcacagct atacgggaga cgacacggtg
     9961 gaaatctcct gccatggcaa tcccagaatc gcggcccgga tcctggaact gctgctgagc
    10021 tcagcccgcc tggcaaaccc aggcgaattt accctcaggg ctatgcacaa caacaagata
    10081 gacctcatcc aggcggaagc gatcaatgac cttatctctg cccccaccag cagatctgga
    10141 tccgcggccc tcagtcaaac ccgcggagtg ctgtcaaata gcttaaaaag catcctggag
    10201 cggatcacgc atgcccggct gcgttgtgaa atggccatcg attttgccga tcaggacctc
    10261 cctcagatcg atacggaggc tctgataaat gatcttgcca ggctggacag cgaggtcagc
    10321 cagctctgtg agcaaggcag ccagggacgc tatatcagag aggggatcag aatctgcctg
    10381 gcaggggctc ccaattcagg taaaagctcg ctcttcaacg cttttcttaa acataaccgc
    10441 gccatcgtaa ctccccatcc cggcactacc agagactatc tggaagagac cctttccctg
    10501 cgaggttacg cgataatttt atatgacaca gccgggttga gagataccag cgacgaggtg
    10561 gaacagcagg gcatcgatcg cagccgggaa ctgatgtttg atgccgatct ggtcctgttc
    10621 ctgattgacg ccagcagact ggcagaacac aaggaagacg atatacccca ggggcttggc
    10681 aataaaatcc agatagtgat cagcaaggct gatctgctgg aaaaagatga tctggaaggg
    10741 ctgctcggca cgggatcgat acccgcgagc gcgattagcc cggatggact ggatgagcta
    10801 tctgaggcta ttctggagcg gtttcaactg gccgaacccc ttccagaacg gcctttggtg
    10861 accaataccc ggcatctggc tgccctcaga agctgcgggg aaggattgca aaacgccctg
    10921 gtagctttga ggtccgggtt gggctttgaa tttgccgcct tcgatctgat ttctgccagc
    10981 gcggccctgg agcaaatcct gggtgtaatc actccggatg acctgctgga gagaattttt
    11041 gccgatttct gcattggcaa ataggacaag gagataatca tgagcaattg gatctattcc
    11101 aaaaacggga aagagtatgg tcccatgagt acttccaaag tggctgatgc catcttgaga
    11161 ggcgaactgg aactggacga ctatgtcctg agcgccgaga ctcagctctg gaagaaagcg
    11221 aaggacgtac aggcgatcat ggatattgtg cataaacccc tgcccaacaa cctctttgac
    11281 gatgtcaacc gggaagagtt ttccagcttt gtcaagggcg acgaatctgt ctatgctgaa
    11341 ccaatctttt tcaatatgcc ctacacgcgt ttgatgatcc tgcaggtgct caccctgggt
    11401 tttttccaga cttactggtt catccgtcag tatatgtatc tgatcaggcg cccgggccgg
    11461 aaacctatgc ggatctcctt gatggtgttc gcgttcgctt tttatatctt caacgaggtg
    11521 gagcacgacc gggagcttaa ttctgcatac cgggcgcctt ggagttcctg gagaatggcc
    11581 atcctctggt atctggtgat tcccttggcg acgtttggat tgcagaccaa caaccctatt
    11641 ctggagatag tgttatcctt gccggtggtg gtctttggca cttccctggt gctcataccg
    11701 gtccagaaat acatcaacga gtgcaacgaa aagct
//
LOCUS       DAOSFN010000075        11559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4691_length_11559_cov_8.973835, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000075 DAOSFN010000000
VERSION     DAOSFN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4691_length_11559_cov_8.973835"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /gene="rplE"
                     /locus_tag="PLO35_06030"
     CDS             <1..414
                     /gene="rplE"
                     /locus_tag="PLO35_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOH79262.1"
                     /translation="QNKALLDNAVKDMEQICGRKVLVTKARKSISNFKLRQGMPIGCK
                     VTLRDEVMYEFYDRLISIVIPRIRDFRGIPADSFDGRGNFSFGLKEQTVFPEIEYDKV
                     DAIRGLNITIVTTAHTDEECRELLSELGMPFQKNQ"
     gene            425..610
                     /locus_tag="PLO35_06035"
     CDS             425..610
                     /locus_tag="PLO35_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOH79263.1"
                     /translation="MAKKSLIIKQQRTPKFQVRKYNRCMICGRPRAFMRDFGMCRLCF
                     RKYASMGQIPGITRSSW"
     gene            760..1173
                     /gene="rpsH"
                     /locus_tag="PLO35_06040"
     CDS             760..1173
                     /gene="rpsH"
                     /locus_tag="PLO35_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOH79264.1"
                     /translation="MSVSDPIADALTKIRNAYRAGHPQVIVNHCGLTESIVKILAAEN
                     FVNSVQVLEKDPEHKINYRRILVILRYTNAGAPVMQGIDRVSKPGRRVYVKATNIPSV
                     YNNTGCAIISTSRGVMVDRDARINGVGGEYICKVW"
     gene            1186..1758
                     /gene="rplF"
                     /locus_tag="PLO35_06045"
     CDS             1186..1758
                     /gene="rplF"
                     /locus_tag="PLO35_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOH79265.1"
                     /translation="MSRIGKSPVKLNDVKVEVSSEGLVAVKGKLGELSYSLQPGITVN
                     IEDNVLHVLRQDDSKSQKALHGLTRALIQNMVIGVSEGYLKTLNVIGTGYSSEVIGPW
                     LKLSLGYSHDIMLQVPAGLTVEAEAVPRSKGTRSDLTSIIRVKGIDKQMVGHFAAEIR
                     GCRPPENYKGKGIRYQDEYVVIKAGKAGSK"
     gene            1772..2143
                     /gene="rplR"
                     /locus_tag="PLO35_06050"
     CDS             1772..2143
                     /gene="rplR"
                     /locus_tag="PLO35_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOH79266.1"
                     /translation="MITPSNIAKIALRSRRRAAIRKRLTGTSTRPRLAVFRSNKHIYA
                     QIIDDSTGKTLVSMSDKAKAFSAEAGLKKTERGMLVGQKLGEVALAAGITKVAFDRAG
                     YKYHGRVKALAEGARKAGLEF"
     gene            2175..2678
                     /gene="rpsE"
                     /locus_tag="PLO35_06055"
     CDS             2175..2678
                     /gene="rpsE"
                     /locus_tag="PLO35_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOH79267.1"
                     /translation="MNLEEDKLVEKIIETKRVAKVVKGGRNFSFSAIVVVGDRAGNIG
                     VGSGKANEIVDAIRKAKEKATKSMFRVPIIKGTIPHEIVARYGASRVMMKPATAGTGV
                     IAGGTARAIFEAAGIQNILCKSLGSNTPCNVVKATVAGLKSMRTISDIARLRNKSIAQ
                     ITGREEK"
     gene            2675..2857
                     /gene="rpmD"
                     /locus_tag="PLO35_06060"
     CDS             2675..2857
                     /gene="rpmD"
                     /locus_tag="PLO35_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOH79268.1"
                     /translation="MKIKVTQIRSIINRKENHKRVIASLGLGRPGKSRVHDDSPSIRG
                     MINKVSYLIKVEELGE"
     gene            2861..3316
                     /gene="rplO"
                     /locus_tag="PLO35_06065"
     CDS             2861..3316
                     /gene="rplO"
                     /locus_tag="PLO35_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOH79269.1"
                     /translation="MLSLHNLGKPAGRKAKKRLGKGQGSGWGHQAGRGHKGRKARSGG
                     TVPAWFEGGQMPMQRRLPKRGFKNIFRVGYRVLNLSRLIGLEEVEYDIVKLEQMGLIP
                     SKGQKGKAPVKVLASLAEEFTLKVNITANAFSKRAKEIIEANGGKAEVV"
     gene            3318..4637
                     /gene="secY"
                     /locus_tag="PLO35_06070"
     CDS             3318..4637
                     /gene="secY"
                     /locus_tag="PLO35_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOH79270.1"
                     /translation="MFKTIMNMFRIPDLKKKILFTALFLVLYRLGSFVPVPGVDTTAL
                     KAFFAGAREGGNTLFGLLDLFVGGNFERASVFALGIMPYITASIVIQLLGSIIPYFEK
                     LKKEGADGQKKMNQITRYATVGLGAFNATTITIWLSSLQGGVVPNSGFLFHFTGVVTL
                     ITGTMIVMWLGEQITEHGIGNGISLIIFAGIIARYPAGFVQLFKLMSTDLNYLWISAV
                     ALVVMVVVTAAVIYVTEAVRKIPVQYAKRIVGRRVYGGQSTYIPLRVNTAGVIPIIFA
                     QSVLMFPATITAMIGDAAKSGFWSELQRLLSPGAWLYTILYVGMIIFFAYFYTAIVLN
                     PTEMAENMVKYGGHIPGKKPGKKTAEYISSVLTRITLPGAVFFAFVALMPEVMSNLFK
                     LPFYFGGTGLIIVVGVALDTLQQIESHLVMRHYDGFMKKGKLRGRSS"
     gene            4639..5409
                     /gene="map"
                     /locus_tag="PLO35_06075"
     CDS             4639..5409
                     /gene="map"
                     /locus_tag="PLO35_06075"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOH79271.1"
                     /translation="MIFLKSTSEITRMRAANRIVAELLNALEPMIRPGANTQELDDFA
                     TRFITERGGRPAFKGYRVPGLNPFPAAICASVNDGIVHGIPSPKVVLKEGDIIGIDVG
                     VELDGYYGDGARTYAVGTISTEARHLMDVTREALTLGIANAREGKRVGDVSHAIGSFV
                     RSHGYYCADSLTGHGIGRDLHEEPQVPNVGQAGRGPRLKAGMTIAIEPMVNIGTNRVK
                     ELGWEFFTADGSLSAHYEHTILITASEAEILTVAGGNQ"
     gene            5406..5624
                     /gene="infA"
                     /locus_tag="PLO35_06080"
     CDS             5406..5624
                     /gene="infA"
                     /locus_tag="PLO35_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOH79272.1"
                     /translation="MSKSGVIEVEGVVTEALPNTTFRVQLENGHEILAHSSGKMRMNY
                     IRILPGDKVKVELSPYDLTRGRITYRYK"
     gene            5663..5779
                     /gene="rpmJ"
                     /locus_tag="PLO35_06085"
     CDS             5663..5779
                     /gene="rpmJ"
                     /locus_tag="PLO35_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004025773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOH79273.1"
                     /translation="MKVRASVKIVCKDCRIIKRHGVIRVICSSNPKHKQRQG"
     gene            5791..6159
                     /gene="rpsM"
                     /locus_tag="PLO35_06090"
     CDS             5791..6159
                     /gene="rpsM"
                     /locus_tag="PLO35_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOH79274.1"
                     /translation="MAHIAGIEIPRNKRLFIGLTYIYGIGPTIAKDICRKANIDEMKK
                     VSDLSVEEEKALRDIVQNDYVVEGALRTQIAMNIKRLMEIGSYRGMRHKRGLPVRGQR
                     THTNARTRKGPRAGAIKKKK"
     gene            6168..6557
                     /gene="rpsK"
                     /locus_tag="PLO35_06095"
     CDS             6168..6557
                     /gene="rpsK"
                     /locus_tag="PLO35_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017433998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOH79275.1"
                     /translation="MAKKTRVKKKRVRLAFDEGLVFVHSSFNNTIISLTDRAGNVLAW
                     SSGGKIGYKGSRKSTPFAAQMAAAEVAKAGMEMGITRVGVIVKGPGGGRESSIRAINA
                     AGIKVTMIKDETPIPHNGCRPPKTRRI"
     gene            6569..7186
                     /gene="rpsD"
                     /locus_tag="PLO35_06100"
     CDS             6569..7186
                     /gene="rpsD"
                     /locus_tag="PLO35_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOH79276.1"
                     /translation="MARYTGPKAKVCRKFGENIFGTTKYDKILNKRKFPAGQHGKNMR
                     RKLSDYGVHLREKQKMRYTYCLLEKQFRNYFVKAAKMAGVTGENLLVMLERRLDNTVY
                     RLGFATTRMQARQFVNHGHFMVNGKKVDIPSYLLKAGDIIEVRPKSRGMEMLKEAMDR
                     TESSSPYSWLSVDKENMRGQFVTIPPASEIPNTVDLRLIVEFYSK"
     gene            7214..8335
                     /locus_tag="PLO35_06105"
     CDS             7214..8335
                     /locus_tag="PLO35_06105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOH79277.1"
                     /translation="MLYLEPLQMPENVEFDSATYTRNFGKVEIGPLEPGFGTTLGNTL
                     RRVLLSSVQGAAVRFVRIEGLHHEFCAIPGTNSDYIDLILRLKQLVIYSSSVEEITVV
                     LEHKGKGIVTASLIQEHPHIKIVNKDLYLLEITEDVDLKIELIIGIGRGYVSADRQNP
                     DGKAVGFIPIDSVYSPILKVNFSVTHQRVKERMNFDKLIMEIHSNGAVEPKYALFLSA
                     KILKDMAAKISLFDTEPEYIRDVELDPELEEKERILRMSVKEIELTVRAANCLTAAKI
                     DTIGDLVSKSEAEMLKFRNFGKKSLEEIMQKLKKYDLELGMDVDGIYSRIREAKSRGV
                     KAEEPAVAEIEESIEEEPTATPRKPVPAPKKKVKHATQS"
     gene            8319..8726
                     /gene="rplQ"
                     /locus_tag="PLO35_06110"
     CDS             8319..8726
                     /gene="rplQ"
                     /locus_tag="PLO35_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOH79278.1"
                     /translation="MRHRVEGRKFGREMDARRLMICNLVKSMVEHGQINTTLAKAKEL
                     RSYVDRIVTYGKNNTVHSRRLAYSVLGSRTLVKKLFDEIAPAFQGREGGYTRVIKAGF
                     RKGDAAPMAIIQFVEESTIKPKKDTIKAKDLNK"
     gene            complement(8880..9167)
                     /locus_tag="PLO35_06115"
     CDS             complement(8880..9167)
                     /locus_tag="PLO35_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOH79279.1"
                     /translation="MCKCGVICATHCGAYKTECEGCLELEGRVSWARFYGKTHCPIYE
                     CVLAKGLANCALCGQAPCQVWNDTRNPDASDAEFAEDINKRLANLSRWLQN"
     gene            9367..11454
                     /locus_tag="PLO35_06120"
     CDS             9367..11454
                     /locus_tag="PLO35_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HOH79280.1"
                     /translation="MRRFTALLLTALCLVALISCVCNVETETPAPVKEVDASSQLEAT
                     GTLETTQETSVKLMLKDAAGAPIQNAVVECYLDSQRIGSLKTDAQGEALFDKLIPGTY
                     SFKVKAEGYKEAELADVKVETNSKLAIEVKAEPVTAVVTEPEVAVVTEPEEVVVTEPE
                     IITDMPDVETIMPTDEIAPQLPVMAEYDMGGYSGKTSIGSVMAAPGGAYPNANLVPTE
                     DWNTEEFSAITPNIFHSPLTEPLSTFSIDVDTASYSTIRRYLNQNQLPPANAIRTEEV
                     INYFSYDYPQPKGEHPFSVYTEMGVCPWNQKRNLVHIGLQGRDLDMASAPASNLVFLL
                     DVSGSMDDPNKLPLVKESMKLLVQNMREQDRVAIVVYAGAAGLVLPSTSGDQKAKIVA
                     ALDNLQAGGSTAGGAGIKLAYGTALENMLRGGNNRVILCSDGDFNVGVSSTNELQQLV
                     EDNRGKGIYLTILGFGMGNYKDNRMEILADKGNGNYAYIDNLMEAKKVLVNEMTGTLF
                     TIAKDVKLQVEFNPAHVKAYRLIGYENRILNAEDFRDDTKDAGEMGAGHTVTALYEII
                     PTASKEEIPGVDPLKYQDIKISEEAQKSPEVMNVKLRYKAPLSDESIPLETPVYNKTL
                     GMEDVSETFMWSAAAAGYAMLLQKSENAAALNWNMLMEWAQSNIGEDKDGYRKEFLDL
                     IKLAAKLSSVELD"
BASE COUNT         3000 a         3256 c         3010 g         2293 t
ORIGIN      
        1 cagaacaaag ccctgttgga taatgcagtg aaggatatgg aacagatctg cggacgcaag
       61 gtgctggtaa ccaaagcccg taaatccatc tccaacttta aactgcgtca gggcatgccc
      121 atcggctgca aagtaaccct gcgggacgaa gtgatgtatg aattttacga tcgcctcatc
      181 tccatcgtaa ttccccgtat tcgcgatttc cgcggcatcc cggccgactc cttcgacggc
      241 agaggaaatt tctcctttgg cctgaaggaa cagactgtat ttcccgagat cgaatacgac
      301 aaggtagatg ccattcgcgg gctgaatatt accatcgtaa ccacagcaca caccgatgaa
      361 gagtgccgtg aactgctcag cgaactggga atgcccttcc agaagaatca ataaggagag
      421 ataagtggcc aagaaatcac tcatcatcaa acagcaacgc acccccaagt tccaggttag
      481 aaaatacaac cgttgcatga tctgcggccg gccccgcgct tttatgcgcg actttggcat
      541 gtgccgtcta tgcttccgca agtatgcttc catgggacag attcccggga ttacccgcag
      601 tagctggtaa atgagaatga cggtaagctc ctgctcgaac tgcggatgca atacgaatga
      661 aatacggatg ggattcgtat ttcaaacgca atgctttccc aattcaagcc ggtttgactt
      721 atccaagaaa gaaaacacac agataaatga ggaaaacaaa tgagtgttag tgatccgata
      781 gctgatgcat tgaccaagat ccgcaatgca tatcgtgccg gacatcctca ggtgatcgtg
      841 aatcactgcg ggctcaccga gtccatagtg aagatcctcg ctgctgagaa tttcgtgaat
      901 agcgtccaag ttctcgaaaa agatccggaa cacaagatca actacagacg tatcctggtg
      961 atccttcggt ataccaatgc cggagctcct gtcatgcagg gtatcgaccg tgtgtcaaaa
     1021 cctggacgcc gcgtttacgt gaaagccacc aatatcccca gcgtgtacaa caacactggc
     1081 tgtgccatca tctccacctc gaggggtgtg atggtggacc gcgatgcccg catcaacggt
     1141 gtgggtggtg agtacatctg caaggtttgg tagggaggta accgaatgtc acgcattgga
     1201 aaatcccccg tcaagcttaa cgacgtaaaa gtggaagtta gcagcgaagg tctcgtggcc
     1261 gttaaaggta agctgggcga gctcagctac tccttgcagc ctgggattac cgtaaatatc
     1321 gaggataacg tgctccacgt cctccgtcag gatgactcca agagccagaa agccctgcat
     1381 ggcctcaccc gcgccctcat ccagaacatg gtgattggcg tgagcgaagg ctacctgaag
     1441 accctcaacg tgatcggcac cggctactca tctgaagtaa tcggtccctg gctgaagctc
     1501 agcctgggct attcacacga catcatgctg caggtccctg ccggattgac cgtcgaggct
     1561 gaagcggttc cgcgttccaa aggtaccaga tccgacctca ccagcatcat ccgggtcaaa
     1621 gggatcgata aacaaatggt tggccatttc gccgctgaaa tccgcggctg ccgtccgccc
     1681 gaaaactaca agggcaaagg catccgttat caggacgaat atgtggtcat caaagccggc
     1741 aaggccgggt ccaagtaaag gggtatgatc aatgataaca cctagcaata tagccaagat
     1801 cgccctccgt tcccgtcgcc gggcagcgat ccgtaaacgc ctgaccggca cttcaacgcg
     1861 tcctcgtctg gcagtttttc gctccaacaa gcacatctat gctcagatca tcgatgacag
     1921 caccggaaag actctggttt cgatgagcga caaagccaag gccttcagtg ccgaagccgg
     1981 tctcaagaag accgagcgcg gtatgctggt gggccagaag cttggcgaag tggccctggc
     2041 agctggcatc accaaagtgg ccttcgaccg cgccggatac aagtatcacg gccgcgtcaa
     2101 agcccttgcc gaaggtgccc gcaaagccgg actggaattc taaggaggat agcttgaatt
     2161 acgaacacag caatatgaac cttgaagaag ataaactggt cgagaagatc atcgagacca
     2221 agcgtgtggc caaagtggtg aaaggcggcc ggaacttcag tttcagcgcc atcgtcgtgg
     2281 tcggcgaccg ggctggaaac atcggggtcg gcagcggcaa agccaatgaa atcgtcgatg
     2341 ccatccgcaa agccaaggaa aaagccacca agtccatgtt tcgcgtgccg atcatcaagg
     2401 gcaccatccc tcacgagatc gtggcccgtt atggtgccag ccgcgtgatg atgaaaccag
     2461 ccacagccgg taccggagta atcgctggtg ggaccgcccg cgccatcttt gaggctgccg
     2521 gcatccagaa tatcctctgc aaatctctgg ggtccaacac cccctgcaac gtggtcaaag
     2581 ccaccgtcgc ggggttgaag agcatgcgca ccatcagtga catcgcgcgt ctgcgcaaca
     2641 agtccattgc ccaaattacc ggcagggagg agaagtgaag atcaaggtca cccagattcg
     2701 cagtatcatc aatcgtaaag aaaatcataa acgggttatc gcctcactcg gattgggacg
     2761 ccccggcaaa agccgcgtcc atgatgatag cccaagcatc cgtggtatga tcaacaaagt
     2821 atcatacctc atcaaagttg aagagctggg agagtaacag atgctgagcc tacacaatct
     2881 tggcaaacct gccggccgca aggccaaaaa acgcctcggt aaaggccagg gcagcggttg
     2941 gggccatcag gccggccgcg gtcacaaagg ccgcaaagcc cgcagcggtg gtaccgtgcc
     3001 cgcctggttt gaaggtggac agatgcccat gcagcgtcgc ttgcccaagc gcggcttcaa
     3061 gaatatcttc cgcgtcggct accgggtgct caatcttagc cgtctgatcg gactggaaga
     3121 ggtcgaatac gacatcgtca agctcgagca gatgggcctc atcccctcca aaggccagaa
     3181 aggcaaagcc cccgtcaagg tgctggcttc cctggccgag gaattcaccc tcaaagtaaa
     3241 catcacggca aacgccttct ccaaacgtgc caaagagatc atcgaggcca atggcggtaa
     3301 ggcggaggta gtgtaacatg ttcaaaacga taatgaacat gttccggatt ccggacctga
     3361 agaagaagat cctcttcacg gccctgtttc tggtgctcta tcgtttgggc agcttcgtcc
     3421 ccgtcccggg agtcgatacc acagcgctga aggccttctt cgccggcgcc agagaaggtg
     3481 gcaacaccct ctttggattg ctggacctct ttgtgggagg aaacttcgaa cgggcctccg
     3541 tgtttgctct gggtatcatg ccctatatca cggcgtcgat cgtgatccag cttctgggct
     3601 cgatcattcc ctactttgaa aagctcaaaa aagaaggcgc ggacggacag aagaagatga
     3661 accagatcac ccgctatgcc acggtaggtc ttggcgcttt caacgccacc accatcacca
     3721 tctggctttc cagcctgcag ggcggcgtgg tacccaacag cggtttcctg ttccacttca
     3781 ccggagtggt gaccctgatc acaggcacca tgatcgtgat gtggctgggt gagcaaatca
     3841 ccgagcatgg aatcggcaac ggcatatccc tgatcatctt cgccggaatt attgcccgtt
     3901 atccagccgg attcgtacaa ctcttcaagc taatgagcac ggacctcaat tacctgtgga
     3961 tttccgctgt cgccctcgtg gtgatggtgg ttgtgaccgc ggctgtgatc tacgtgaccg
     4021 aagccgtccg caagatcccc gtccaatatg ccaaacgcat cgtgggacgc agggtctatg
     4081 gtggccagag cacctatatc ccgctccggg tcaacaccgc gggtgtgatt cccatcatct
     4141 ttgcccaatc cgtgctgatg tttcccgcca ctattactgc catgatcggc gacgctgcca
     4201 agagtggttt ctggtctgag ttgcagcgcc ttctcagtcc cggtgcctgg ctctacacca
     4261 tcctgtatgt gggaatgatc atcttcttcg cctatttcta tacggcgatc gtgctcaacc
     4321 ccaccgagat ggctgaaaac atggtcaaat acggtggaca catcccaggc aagaaacccg
     4381 gtaagaagac tgccgagtat atcagcagcg tgcttacccg catcaccctt ccgggcgcag
     4441 tgttctttgc ctttgtagct cttatgcctg aagtcatgag caacctcttc aagctgccat
     4501 tctactttgg cggcaccggt ctgatcatcg tggtcggcgt ggcgctggat actctccagc
     4561 agatcgaaag ccacctggtg atgagacact atgacggctt catgaaaaaa ggcaagctgc
     4621 gcggacgttc cagctaatat gatattcctc aaatccactt ccgagatcac caggatgcgg
     4681 gccgccaacc gaatcgtcgc agagcttctg aatgctctgg aaccgatgat ccggcctgga
     4741 gccaataccc aggagctgga tgactttgcc acccgtttta tcacagagcg gggcggccgg
     4801 ccagccttca agggctaccg ggtgcctggt ctgaatccgt ttccggcggc catttgcgcc
     4861 tccgtgaacg acgggatcgt gcacggcatc cccagcccca aagtggtcct caaggaaggt
     4921 gatatcatcg gcatcgacgt gggagtggaa ctggacggat actatggaga cggagcccgc
     4981 acctatgcgg tggggaccat atccacggaa gcccgtcatc tgatggacgt tacccgcgaa
     5041 gccctcaccc tcggcatcgc caatgccagg gaaggcaaac gggtgggaga tgtctctcac
     5101 gccatcggaa gcttcgttcg ctctcacggc tactactgcg cggacagcct taccggtcat
     5161 gggatcggca gggacctgca cgaagagcct caggtgccaa acgttggcca agctggccgt
     5221 ggtcccaggc tcaaagccgg gatgaccatc gccatcgaac ccatggtgaa tatcggtacc
     5281 aaccgggtca aagaactggg ttgggagttt ttcacggcgg acggaagtct ttccgcccac
     5341 tatgagcata ccatcctgat taccgccagc gaggctgaaa tactcacagt ggcaggagga
     5401 aatcaatgag caaatccggc gttatagaag tggaaggcgt cgtcaccgaa gcccttccca
     5461 acactacctt ccgcgtgcaa ctggaaaatg gccacgagat tctggcccat tcttccggca
     5521 aaatgcgcat gaattatatc cgcatccttc cgggtgacaa ggtcaaggtg gaactcagtc
     5581 cctacgacct tacccgcgga cgcataacat accgctataa ataacactgc ccatacatta
     5641 tgtttggagc aaggagatca taatgaaagt acgagcatca gtcaagatag tctgcaaaga
     5701 ctgccgtatc atcaaacgcc acggcgtgat ccgtgttatc tgcagttcaa atcctaaaca
     5761 taaacagaga cagggataag gaggaccaac ttggcacaca ttgcaggtat agagattccc
     5821 aggaacaaac ggctgttcat tggtctcacc tacatctatg gcatcggtcc cacgatcgcc
     5881 aaagatatct gccgcaaggc caatatcgac gagatgaaga aggtgtctga cctcagcgtg
     5941 gaagaagaaa aagctctacg tgacatcgtc cagaacgact atgtggtcga aggtgccctc
     6001 cgcacccaaa tcgccatgaa tatcaagcgt ctgatggaaa tcggcagcta tcgtggaatg
     6061 cgccacaaac gcggtctgcc ggtccgtggc cagagaacac acaccaacgc ccgcacccgt
     6121 aagggtcccc gcgccggtgc catcaagaag aagaaatagg aggggaaatg gccaagaaga
     6181 ccagagtaaa gaaaaaacgc gtccgccttg cctttgacga gggcctcgtc tttgtgcatt
     6241 ccagcttcaa caacacgatc atttcgctca ccgaccgtgc cggaaacgtc ctggcctggt
     6301 ccagcggagg caagatcggt tataaaggct ctcgcaagag cactcccttt gccgctcaga
     6361 tggctgccgc ggaagtggcg aaagccggaa tggagatggg aatcacccgc gtcggcgtga
     6421 tcgtgaaagg ccctggcgga ggacgtgaat cctccatccg cgccatcaac gccgccggga
     6481 taaaagtcac catgatcaag gacgaaaccc ccatcccaca caacggctgc cgtcccccca
     6541 agaccagaag gatataagcg aggagcacat ggcaagatat accggaccca aagcaaaggt
     6601 ctgccgcaag tttggagaaa acatcttcgg caccaccaaa tacgataaga tcctgaacaa
     6661 acgcaagttc cccgccggac aacacggcaa gaacatgcgt cgtaagctga gtgactatgg
     6721 agtgcatctg cgcgaaaagc agaaaatgcg ctacacctac tgcctgctgg aaaagcaatt
     6781 ccgcaactac tttgtcaaag ccgccaaaat ggctggtgta acaggcgaaa acctgctcgt
     6841 aatgctggaa cgccgtctgg acaacaccgt ctatcgtctt ggctttgcca ccaccaggat
     6901 gcaggcccgt cagttcgtca accatggcca tttcatggtc aacggcaaga aagtggacat
     6961 cccgtcctac ctcctcaaag ccggagacat catcgaagtg cgccccaaga gccgtggcat
     7021 ggaaatgctc aaggaagcta tggaccgcac cgagtccagc tctccttaca gctggctttc
     7081 ggttgacaaa gagaacatgc gcgggcagtt cgtcaccatc ccacccgctt ccgaaatacc
     7141 caataccgtg gatcttcgcc tgatcgtcga gttctactcc aagtaatgct ctaacccaag
     7201 caaggagtca attatgttgt accttgaacc tttacagatg ccggagaacg tggaatttga
     7261 ttccgcgacc tatacccgta acttcggcaa agttgagatc gggcctctgg aacccggatt
     7321 tggcaccacc ctcggcaaca cgctgcgcag ggtgctgctc tcttccgtgc agggagccgc
     7381 ggtacgcttt gtgcgcatcg aaggtctgca tcacgagttt tgcgccatcc ctggcaccaa
     7441 ctctgattac atcgacctca tcctgcggct caaacaactg gtgatctatt cttccagcgt
     7501 tgaggaaatc accgtggtgc tggagcataa aggcaaaggc atcgtcaccg cttctctcat
     7561 ccaggagcat cctcacatca agatcgtcaa caaagatcta tatctcctgg aaatcaccga
     7621 agacgtggac cttaaaattg agctcatcat cgggatcgga cgcggctacg tgagcgctga
     7681 ccgccaaaac ccggatggca aagcagtggg cttcataccc atcgacagcg tctattcacc
     7741 catcttaaaa gtgaatttca gcgtcactca ccagcgtgtg aaagaacgca tgaacttcga
     7801 caagctcatc atggaaattc actccaacgg cgctgtggaa cccaaatacg ccctcttcct
     7861 atctgccaaa atcctcaagg acatggcagc caagatcagc ctcttcgaca ccgaaccgga
     7921 atacatccgc gacgtcgaac tcgatcctga actggaagag aaagaacgca tcctgcgcat
     7981 gagcgtcaag gaaatcgaac tcacggtccg cgcggccaat tgcctcaccg cggccaagat
     8041 cgacaccatc ggcgatctcg tctccaaaag cgaggccgag atgctcaaat tccgcaactt
     8101 cggcaagaaa tcgctcgaag agattatgca gaaactcaag aaatacgacc tggaactcgg
     8161 tatggacgtg gatggcatct actccagaat ccgcgaagcc aaaagccgcg gcgtcaaagc
     8221 tgaggaaccg gccgtcgccg aaatcgaaga aagtatcgaa gaagaaccga ccgccactcc
     8281 ccgcaaacca gttccagccc ccaaaaagaa ggtgaaacat gcgacacaga gttgaaggta
     8341 gaaaattcgg ccgcgaaatg gacgccagaa ggctcatgat ctgcaacctc gtcaaatcca
     8401 tggtcgagca cggacagatc aacaccaccc tcgccaaagc caaggaactg agaagctatg
     8461 tggacaggat tgtgacctac ggaaaaaaca acaccgtcca ctccagacgc ctggcctaca
     8521 gcgtgctggg aagccgcacc ctcgtcaaaa aactctttga cgaaatcgct cccgctttcc
     8581 agggccgcga aggtggctac accagagtca tcaaagccgg cttccgtaaa ggcgacgccg
     8641 cccccatggc catcatccag ttcgtggaag aatccaccat caaacccaaa aaagatacca
     8701 tcaaagccaa agacctgaac aaatagtctc tggcctgaaa gcgataaagc ggctttccga
     8761 tctgcggaag gccgcttttc tataggggcg cgatttccag gtcggggtgt gggtctgatg
     8821 cagacgccaa agaaagaccg ctggtatagc agagacaatg gctccaaaac cagttatatc
     8881 tagttttgca accacctgct cagattggcc aggcgcttgt ttatgtcctc agcgaattcc
     8941 gcgtctgacg catcgggatt gcgggtatcg ttccagacct ggcaaggtgc ctgtccacag
     9001 agggcgcaat tggccaggcc tttggccagc acgcattcat agatggggca gtgggttttg
     9061 ccgtaaaacc gggcccagga taccctgccc tccagttcca ggcagccttc acactcggtc
     9121 ttgtaagctc cgcaatgggt ggcgcagatg acgccgcatt tgcacatatt caagtatcac
     9181 ctctcaaatc tcgttgtgta gatggaataa gcattcctgc gtaaacgtgg acgcttgcgt
     9241 atccaaaaag cgagaaaagg cttgtttgtc aatcactttc actttctgag ggcgatttag
     9301 cttgacatgc cacggccctg ggtttatggg gtcagacagg taatttttga atcccaggga
     9361 ggataaatga gaagatttac tgcgctgttg ctcacagctc tatgcctggt cgccctgatc
     9421 agttgcgtgt gcaacgttga aaccgaaacc cccgccccag tcaaggaggt ggatgcttca
     9481 tctcagcttg aggctaccgg aacgttggag acaactcagg aaacgagtgt caagcttatg
     9541 ctcaaagacg ccgccggagc cccgatccag aatgccgtgg tagagtgtta tctggacagc
     9601 cagcggatag gttctctaaa gactgatgcc cagggtgaag cgctgtttga caagctgatc
     9661 cccggcacct acagtttcaa agtgaaggcc gaaggttaca aagaagctga actggccgat
     9721 gtaaaagtcg agaccaattc caagctggcc atcgaggtta aagccgaacc cgtaacagca
     9781 gtggtcaccg agccagaagt ggcagtggtc accgagccag aagaagtagt ggtaacagag
     9841 cctgagatca tcaccgatat gccggatgtg gaaaccatca tgcctacaga tgaaatcgct
     9901 ccccaactgc ctgtcatggc cgaatacgac atgggcggtt attctggtaa aaccagtatt
     9961 ggatccgtaa tggcagcccc aggaggagct tatcccaatg ccaatctggt ccctactgaa
    10021 gattggaaca ccgaggagtt tagcgccatt actcccaata tcttccacag cccgctcacc
    10081 gagccgctgt ccaccttttc catcgacgtc gataccgctt cctacagcac catccgtcgc
    10141 tacctcaatc agaatcagct ccctcccgcc aacgcgatcc gcactgagga agtgattaac
    10201 tatttcagct atgactatcc tcagcccaaa ggcgagcatc ccttcagcgt ctataccgag
    10261 atgggcgtct gcccctggaa ccagaagcgc aacctcgtgc atatcggatt gcagggcagg
    10321 gacctggata tggccagcgc gccggccagc aatctggtct ttttgctcga cgtgtccggt
    10381 tcgatggatg atcccaacaa gctgccgctg gtgaaagaat cgatgaaact actggtgcaa
    10441 aacatgcggg agcaggatag agtagcgatc gtggtctatg ccggagcagc gggattggtg
    10501 ttgccatcca cctccggaga tcagaaagcc aagatcgtcg ccgccctgga taatcttcag
    10561 gctggaggtt ccacagccgg tggagctggc atcaaactgg cctacggcac cgcgctggaa
    10621 aacatgctca ggggtggaaa caaccgtgtg atcctttgct ccgacggtga cttcaacgtg
    10681 ggcgtctcca gcaccaacga actgcagcaa ctggtggaag acaaccgcgg caagggtatc
    10741 tatctcacca ttctgggctt tggaatgggc aattacaagg acaaccgcat ggaaatcctg
    10801 gccgacaagg gcaacggcaa ctatgcctat atcgacaacc tcatggaagc caagaaagtg
    10861 ctggtcaacg agatgaccgg aacgctcttt accatcgcca aagacgtcaa gctccaggtg
    10921 gagttcaatc ccgcccacgt aaaagcctac cgcctgatcg gttatgaaaa ccgcatcctc
    10981 aacgccgaag actttagaga cgacaccaag gatgccggtg aaatgggtgc cggtcatacg
    11041 gttacggccc tctatgagat catccccacc gcctccaaag aagagatccc aggcgtcgat
    11101 cccctcaaat atcaggatat caagatcagc gaagaagctc agaaatcccc tgaggtaatg
    11161 aacgtcaagc tgcgctacaa agcgcctcta agcgatgaaa gcattccgct ggagaccccg
    11221 gtttacaaca agaccctcgg catggaagac gtgtctgaga cctttatgtg gtccgccgcc
    11281 gcggcaggat atgccatgct gcttcagaag agcgaaaacg ccgccgcgct gaactggaac
    11341 atgctgatgg aatgggccca aagcaacatc ggtgaagata aagacggcta ccgcaaggaa
    11401 tttctggacc tcatcaagct ggctgccaag ctcagcagtg tcgaactgga ttaaacaaac
    11461 atagtcagtt aagtataaag ggccggccct gaggggctgg ctcttttgtc gttaagcgtt
    11521 atgcgttatg cgtgatgcga accctctgcg cggagctta
//
LOCUS       DAOSFN010000076        11523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4713_length_11523_cov_9.826474, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000076 DAOSFN010000000
VERSION     DAOSFN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4713_length_11523_cov_9.826474"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLO35_06125"
     CDS             complement(<1..359)
                     /locus_tag="PLO35_06125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOH79281.1"
                     /translation="MLSKILRRTAYLILSAIDKLPAPKPAPGTAMTCPFCSIPPEKYL
                     ASNDYFYAIPDHRPVSRGHSLVISKRHVETYFELSPEEAAALNALVSEVKTILDQRHK
                     PKGYKLLMNCGASAGQRV"
     gene            complement(359..>1182)
                     /locus_tag="PLO35_06130"
     CDS             complement(359..>1182)
                     /locus_tag="PLO35_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79282.1"
                     /translation="PRIDILADEALSGRLGRELAKTGSIGPEATSEALSVIRDIQVRI
                     GTWKPVNTLCVGAMTLRTAQDAPEFIQALYQATGLRARVLSGDEEARLAWKAATMRLP
                     VLADQAVLDIGGGSFELILGSDQPAFSHSFPLGALTLKDQCICHDPPLETELEAAREL
                     VKTQLHQQSVPEKASALIAIGGTAVNLAAIQRSLESKPDLEGSVISVTELERMLKLFS
                     GMTLAQRRKLPGLTPSRADIIIPGTLILSEVLDHLGLSSFRVSTRGIRHALLTEL"
     assembly_gap    1183..1282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1363..3627)
                     /locus_tag="PLO35_06135"
     CDS             complement(1363..3627)
                     /locus_tag="PLO35_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOH79283.1"
                     /translation="MKKLPWHIAGPLHVTGRSHFIADAAPLPGMLHAKILFSPVAHAR
                     LISIDTHAALLPGIHSIIGAEDIPAANQIGHVIKDEPLFPTERIMYLGQPLLLVLAED
                     ERLAEAALKRIKLEYEELPPLLSIDAADAAHEWYIPERRIEKGSVDQAMMDAAFVLAG
                     ESLTAGQEHFYMEGQRCRAIPGEGHTLTIQTATQSTMEVQEVLSHVLGIPAHDLIIEV
                     PRLGGAFGGKERAATLWACLAGLAAHLSGRPVQIKLSRDEDMLVTGKRHPFKSRWRIG
                     FDAKGIIQAWDIELMSNGGAYADLSVAILERALFHAENAYHIPHARIRGRACRTNLPP
                     NTAYRGFGAPQGIFVIEDAIERVAVKLQLDPLEIRLRNAYKNGDITPYGQPVCESGAE
                     RTMLQVAENADYKQLRAEVASFNASHEFCQQGIGIMPVKFGISFTTALLNQGSALIWT
                     YIDGSVSVSTGGVEMGQELNTKVALVVAKVLGIPMAQIRVESSNSQRVGNASPTAAST
                     GSDINGNAARIAATQIKERLTPLALKLLAQEHGIIVSEEELIWQGGMIYSTHESGKQI
                     SFGELVRYAYNERVPLGAHGYYRTPNLHFDREKGVGHPFHYFVYGCAISRVQVDLLSG
                     EHQVLKTWIVHENGLSLHPEIDRGQITGAFVQSHGWCTMEELPVNDKGRYLAVNPSTY
                     KIPTVRDLPLELDISILESTGEQASVFGSKAVGEPPFIYGMSVWFAIRDAIGGRELAF
                     PATPEAVLMAVQTE"
     gene            complement(3624..5120)
                     /locus_tag="PLO35_06140"
     CDS             complement(3624..5120)
                     /locus_tag="PLO35_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOH79284.1"
                     /translation="MSQNTLYPAHGETREYLYNRIGFWLNGQYLEDDIPPAMTVMDYL
                     HQVANLWGTKCSCNEGDCGACTVAIAYPKEGNIVYEAVNSCLYTAAKLHGKHLITVEG
                     LGSPEALHPIQSALLDWHGSQCGYCTPGFVMSLFALLASVLRPGEEDILAALEGNLCR
                     CTGYDSILKAARHLSEHFTPEQILPPWCRGIESLLFNYKIEPAMLSISSGRVWPSRQY
                     HTPRALDQLWPLMAQNPDYIIIRGGTDIMVQMNIQRKEFATLLDLSMIPELDKISYTP
                     QGIVIGANVTYSDIMASGVVKADLPCLSEIIRLIASRQIRNFGTLIGNIANASPIGDS
                     IPLLLALESVLVISSSEGDKELPLSEFFLSYRHTALQPGEIIRAIRVPIPAKTAFIRV
                     KKAAKRKAVDISSVASALRIETQDGIVKQAFLAVGGMDAIPRLSVRFHNALSNMELQG
                     LHAAEIAAWVAEEFQPLTDVRGSAEYRSKALQMQVLEYLNEFLGGRRS"
     assembly_gap    5265..5364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5652..6716)
                     /locus_tag="PLO35_06145"
     CDS             complement(5652..6716)
                     /locus_tag="PLO35_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015737868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOH79285.1"
                     /translation="MRHLLTVTLVLMFLPLVADSFPTPQLPWGPLTYTACQTTGELLM
                     DGRLDEPDWQAAPWTADFVDIEGDLKPAPFLRTRAKLLWDKDFLYIGAELEEPHIWAT
                     LSQRDAVIFHDNDFEVFIDPDGDTHDYYELEINAMGTLWDLLLIKPYRDRQQVAVNAW
                     DIREIEYGIHIDGSLNDPSDLDKAWFVELKIPLSTLAECAHKPVPPLQGDWWRLNFSR
                     VHWQTEGVDGSYRKIPGRPECNWVWSPQGLIAMHYPERWGYLFFAHAPSGSEISFAIP
                     ALEHRKEALRQLYYLQKEHHAQKGRYARSLRSLAIPEALMALLSPAPRIESTSQSYII
                     TLPATDEFPALHIREDGLLW"
     gene            complement(7124..8089)
                     /locus_tag="PLO35_06150"
     CDS             complement(7124..8089)
                     /locus_tag="PLO35_06150"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOH79286.1"
                     /translation="MKRHPLSPILTRKDILGIDSRLRDVSSVFNPGGIRWQGKTTLLL
                     RVQNRARQTLLVKAISKDGLKFVTDVKPVSFHGVKGFADRIFHIYDPRIVRIGERFLV
                     TCAMDTESGCRVGIFGSEDLEHLEFISLLSDADQRNGVLFPELIDGRYLMLSRPNEIT
                     GPDGVRSGDRIGCYSSADLYSWQEEGELMKGQPHYWDELIGSGPPPIRTPIGWLHIYH
                     GVATHFGGGGIYQAGLSLLDLKEPWKTIVRGPYNVLEPREPYETCGQVPNVVFPTAAI
                     PMTDAEVDLDTPIYLYYGAADTCVGLAVSTIQDLLLYSGIELSFT"
     gene            complement(8086..9519)
                     /locus_tag="PLO35_06155"
     CDS             complement(8086..9519)
                     /locus_tag="PLO35_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOH79287.1"
                     /translation="MIPIPTIVKETGGSFRIPVNVRVYCDGLDPIQESIYEQSLTDWL
                     ETLKKEARYQAPLAGLSIEISIDSEGDEDTDEYVITLTRQVIKIMAPGSHGIYYGIQT
                     LKQLMWEAIWHNQGRIKCGTIYDKSVFPWRGLHLDVSRHFFSAEEVMTYLDWMGALKL
                     NCFHWHLSDDQGWRIESKRFPKLTEKGAWRREADGSRYGGFYTRDEIEAILLKAALNC
                     INVIPEIDLPGHAMAILSAYPELACFPQEFEPLSVWGISEDILCAGKDATLDFLKELL
                     SEVAELFPSPIIHIGGDEAPKARWKACPHCQQRIKDHKLKDEEALQGWLIRELRKHLR
                     KLGKEIMGWDEILDGNPGKGPLISCWRGDGIAAARKASQNGNPYVICPNQFLYFDWKD
                     SEDGPGAFGVTSLEKVMSLKPEDYEFDRPELLLGGQANLWTEHMEDFDKVEEMLFPRV
                     YALSELFWAGKIESGFEERMGRMEDLF"
     gene            complement(9516..>9720)
                     /locus_tag="PLO35_06160"
     CDS             complement(9516..>9720)
                     /locus_tag="PLO35_06160"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HOH79288.1"
                     /translation="GLVLRFYEYRGASAELEFPIPKGYLEPRLCDLRERPLDEAVELR
                     DHHIILRFRPFEIKTLRLDKEKR"
     assembly_gap    9721..9820
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9821..11484)
                     /locus_tag="PLO35_06165"
     CDS             complement(<9821..11484)
                     /locus_tag="PLO35_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOH79289.1"
                     /translation="MKEIYPDLWQEVKTAVEQDRWEVQGAGWVEFDTNLPSGESIIRQ
                     LCYGLRYFEEELGQSPKGLWLPDCFGFNANLPQLLKGCGLQWFMTQKLSWNESNVFPH
                     HLFVWEGIDGSQILAHQLPTNDYNFSNNPSSFLQSEARYAQGELCDAFLNLYGIGDGG
                     GGPTREHLEYGLRQRDLEGVPRFRFAKGQDFYDHIKILDKALLPKLFGELYLEFHRGT
                     YTTQALMKKHNRQSERLLSAAEFVAALSGNDYPGELRDIWEDTLLLQFHDIIPGSSIG
                     QVYEDAHALSEANHQRLKDFISSGIKAAVASEEEARQPVYAVLNPCAWSKEAWLEFPL
                     EQLESVAVDGQGRELPALQFPDKLLVKADLAPWSLSQIRWEAGRCNYQVALPTDELLM
                     ENEQLKVKLSPRGTIISIILKAEGREMLAGESNLLKLWEDEPNNWGAWDINHFYRDTV
                     PMEASQVRLLEDRSWKAGSHLQRLCFELQIGLSTVVQTYELSDAAPYLRISHAVDWKE
                     HHKMLRAHFYPDVHADQASYEIAMGTLKRSTKPSNSFEEARFEVPA"
BASE COUNT         2241 a         3167 c         3292 g         2523 t
ORIGIN      
        1 acccgctgtc ccgcggacgc tccacaattc atcaagagtt tgtagccctt gggcttatgc
       61 cgctggtcca ggatggtttt gacttcgctc accagggcat tgagggcggc agcctcttcc
      121 ggactgagct cgaagtatgt ctccacgtgg cgtttgctga tcacgaggct gtgacctctg
      181 gaaacgggac ggtgatccgg aatggcgtaa aagtagtcgt tggaagccag atatttttct
      241 ggaggaatgg agcagaatgg acaggtcata gcggttcccg gggccggttt gggggccggc
      301 agtttgtcga tggcgctcag gatcagatag gcggtccggc gcaggatttt gctgagcatc
      361 aaagctccgt caggagggcg tgacgaatgc ccctggtgct cactctaaag ctactcaggc
      421 caagatgatc cagcacttca gacagaatca gcgtcccagg gatgatgata tcggcccggc
      481 tgggtgtgag gcccgggagt ttgcgacgct gggccaaggt catgccagag aagagcttga
      541 gcatccgctc cagttctgtg acggagatga ctgagccttc caaatccggt tttgattcca
      601 gtgatctttg gatggcggcc aggttgacgg ccgtccctcc gatggcgatc aaagcggagg
      661 ccttttctgg tacgctttgc tgatggagct gagttttcac cagttctctg gccgcctcca
      721 gttccgtttc cagaggaggg tcgtgacaga tgcattggtc tttgagggtc aaagctccga
      781 ggggaaagga atggctgaag gcgggttggt ccgagcccag aatgagctca aaagagccgc
      841 ctccgatatc cagtacggct tgatcggcaa ggacgggaag cctcatggtg gcggctttcc
      901 aggccagcct ggcctcttca tccccgctga ggacgcgggc ccgcagtccg gtggcttgat
      961 agagggcctg gataaactct ggcgcgtcct gggccgtcct cagagtcatg gcacccacgc
     1021 agagtgtgtt tacgggcttc caggtcccga tccggacctg gatatctctt atcacggaaa
     1081 gcgcctcaga cgtggcctca gggccgatag aaccggtttt ggcaagctct ctgcccagcc
     1141 tgccgctcag ggcctcgtcc gcgagaatgt ctatacgagg cannnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nngtctgcgg ctccactatg gccaccacat acttgaaggt
     1321 gttggtgccg aggtcgatga cggccaggac ggctttagcc attcactctg tctggactgc
     1381 catcagcacc gcttccggtg tggcgggaaa ggccagttcc ctgcctccga tggcatcccg
     1441 gatggcgaac cagacgctca tgccatagat gaaaggcggt tcgcccacgg ccttgctgcc
     1501 aaacacgctg gcctgctcgc cggtcgattc caggatggaa atatccagtt ccagaggcag
     1561 atcgcgcacg gtgggaattt tgtaggtgga tggattcaca gccagatagc ggcctttgtc
     1621 gttcaccggc agctcctcca tggtgcacca tccgtggctc tggacaaagg caccggtgat
     1681 ctggccgcga tcgatctcag gatgcaggct caggccgttt tcgtggacga tccaggtttt
     1741 cagcacctga tgctctccgc tcaggaggtc cacctgcacc ctcgagatgg cacagccata
     1801 cacgaagtag tggaagggat ggcccactcc cttttcgcgg tcgaaatgca gattgggggt
     1861 gcgatagtag ccatgcgcgc caaggggaac gcgttcgttg taggcatagc ggaccagctc
     1921 gccaaagctg atctgcttgc cggattcgtg ggtgctgtag atcatcccgc cttgccagat
     1981 cagctcttcc tcgctcacga tgataccgtg ttcctgggcc aacagcttga gggccagagg
     2041 ggtgagccgt tctttgatct gagtggcggc gatccgggcg gcgttgccgt tgatgtccga
     2101 tcccgtggaa gcggctgtgg gagaggcgtt tcccactctc tggctgttgc tggattccac
     2161 cctgatctgg gccatgggga tgcccagcac tttggcgacg accaacgcca ctttggtgtt
     2221 gagttcctgc cccatttcca cgcctccggt gctgacggac acggagccgt cgatataggt
     2281 ccagatcagc gcggagcctt gatttagcaa cgcggtggtg aaggagatgc caaacttgac
     2341 cggcatgatc ccgatgccct gttggcagaa ttcgtggctg gcgttgaagc ttgccacctc
     2401 ggcgcggagc tgtttatagt ccgcgttttc agccacctgg agcatggtcc gctccgctcc
     2461 ggattcgcag acaggctgtc cgtagggagt gatgtcgcca tttttgtaag cgttgcgcag
     2521 cctgatttcc agaggatcga gctggagttt gacggcgacg cgttcgatgg cgtcctcgat
     2581 cacgaagatg ccctggggag cgccaaaacc tctgtaggcc gtgttgggag gcaggttggt
     2641 gcggcaggcg cggcctctga tcctggcgtg ggggatgtga taggcgtttt cggcatgaaa
     2701 gagagcacgt tccaggatcg ctacactgag atcggcgtaa gcgccaccgt tgctcatcag
     2761 ttcgatatcc caggcctgaa tgatcccttt ggcgtcaaag ccgatccgcc aacggctctt
     2821 gaaaggatgg cgtttcccag tgaccagcat gtcttcgtcg cggctcagtt tgatctggac
     2881 gggacgtcca ctcagatggg ccgccagccc tgccaggcac gcccaaagcg ttgccgctct
     2941 ttcttttccg ccaaatgctc cgcccagtct gggaacctcg atgatgagat cgtgggccgg
     3001 gatgcccagc acgtgagaca gcacttcctg cacctccatg gtgctctggg tggccgtctg
     3061 gatggtgagg gtatggccct cgccagggat ggcccgacag cgttgtcctt ccatgtaaaa
     3121 gtgttcctgt ccggcagtta gcgattcacc ggccagcacg aacgcggcgt ccatcatggc
     3181 ctggtccacg ctgccttttt cgatgcggcg ctccgggata taccactcgt gagctgcatc
     3241 cgcggcgtcg attgacagga ggggcggcaa ctcttcatac tccagcttga tcctcttgag
     3301 agccgcctcc gcgaggcgtt catcctctgc cagcaccagc aacagaggct ggcccagata
     3361 catgatccgc tcggtgggga agaggggttc gtccttgatc acgtggccga tctgattggc
     3421 ggccgggatg tcctcagccc cgatgatgct gtggatacca ggcagcagag cggcatgggt
     3481 gtcgatggaa atcaggcgcg cgtgggctac cggagaaaag aggattttgg catgcagcat
     3541 gcctggcagg ggagccgcgt cggcgataaa gtgagatctg ccagtcacat gcaagggacc
     3601 ggcgatgtgc cagggaagct ttttcatgag cgcctcccgc ccagaaactc atttagatat
     3661 tccaatacct gcatttgcaa ggctttggag cgatactccg cggaacctct cacatcggtg
     3721 aggggctgga attcctctgc cacccaggcc gcgatctcag cggcatgcaa gccctgcagc
     3781 tccatgttgc tcaaggcgtt gtggaatctg acgctcagcc tcgggatggc atccatacct
     3841 cccacggcca gaaaggcctg cttcacgatc ccgtcctggg tctcgatcct cagcgcgctg
     3901 gccaccgagg agatatccac cgccttgcgt ttggcggctt tctttactct gatgaaggcc
     3961 gttttggcag ggatgggaac gcggatggcg cggatgatct ctcccggctg gagagcggta
     4021 tggcgatatg aaaggaagaa ctcactgagc ggcagctctt tatcaccttc ggaggagctg
     4081 ataacgagca cgctctccaa agccagcaac aaggggatgg aatccccgat gggagaggcg
     4141 ttggcgatat ttccgatcag agtgccgaag ttgcggatct gtctggaggc gatcagccgg
     4201 atgatttcag agaggcaggg cagatcggct ttgaccactc cggaggccat gatatcgctg
     4261 taagtcacat tggcgccgat cactatgccc tgaggggtgt agctgatctt atccaactca
     4321 gggatcatgc tgaggtccag aagggtggcg aattccttcc tctggatgtt catctgcacc
     4381 atgatgtctg tcccgccgcg gatgatgata tagtctgggt tttgggccat caggggccag
     4441 agctggtcca gagcgcgggg agtgtgatac tgtctggagg gccaaacgcg cccgctggag
     4501 atgctcagca tagccggctc tattttatag ttgaagagca gggattcgat gccccggcac
     4561 cagggcggca ggatctgctc gggtgtgaaa tgctcactca agtgtctggc tgctttgagg
     4621 atagagtcgt acccagtgca gcggcagagg tttccttcca gggcggcgag gatgtcttcc
     4681 tcaccgggtc tcaggacgct ggccaggagg gcaaagaggc tcatcacaaa gccgggggtg
     4741 caatatccgc actgagagcc atgccagtcc aaaagcgcgc tctggatggg atgcagggct
     4801 tccgggctgc cgagtccctc caccgtgatc agatgcttgc catgcagttt ggcggccgtg
     4861 tagagacagg aattcaccgc ttcatagacg atgttgccct ctttgggata ggcgatggcc
     4921 accgtgcagg ctccgcaatc gccttcgttg caactgcatt tggtgcccca gagattggcc
     4981 acctggtgca gataatccat caccgtcatg gcgggcggaa tatcgtcctc aagatattgt
     5041 ccgttcagcc agaagccgat ccggttgtaa aggtattcac gggtttcgcc atgggcgggg
     5101 taaagcgtgt tttggctcat atataccacc tttaactggt tcgaactacg atatccgggc
     5161 gctggatttc tgtaaagaaa aatctgtgct tgggaggttc agagggatgt ttactcttat
     5221 aaaacacaga gaaaagcgga ggattggcag agaaaagcag agttnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnncgtttg ccgtggcccg cagggacact ctctctgctt
     5401 ttctctgccg ttcctctgct tttcgcagtg ttttaatcgc gatgcgttac gcgttatggg
     5461 cacgcgccgg attggaatcc gacgctatga tcccacggaa acgaggacgt ttccggttcc
     5521 ataatcatcc ctctctgtag ctctctctgt gtcctccgtg gcctctgtag tgaaaaaaga
     5581 atcctctgta tctcagtgtt aatctgcgtg atctgcgctc aaatccgcgt aatctgcggg
     5641 aaattcaggc ctcaccacag caaaccgtct tctctgatgt ggagagcggg aaattcatcc
     5701 gtggcgggca gcgtgatgat atagctctgg gaggtgctct cgattctggg agcgggactt
     5761 agcagtgcca tcagcgcttc cgggatggcc aggctcctga gtgatctggc atagcgtccc
     5821 ttttgggcgt gatgctcttt ttgcaggtag tagagctggc gcagggcttc tttgcggtgc
     5881 tccagagctg gaatggcaaa gctgatctca gatccggacg gtgcgtgggc aaagaagaga
     5941 tagccccagc gctccggata gtgcatggcg atcaggccct gcggactcca aacccagttg
     6001 cattcaggcc ggccagggat tttgcgatag cttccgtcca ccccttccgt ctgccagtgc
     6061 accctcgaaa agttaagccg ccaccagtct ccctgcaaag gtggaactgg tttgtgagcg
     6121 cattcggcca gcgtgctcag gggaattttc agctctacaa accaggcttt gtcaagatcg
     6181 gagggatcgt tgaggctgcc gtcgatgtgg atgccgtatt cgatctcacg gatgtcccag
     6241 gcattgacgg ccacctgttg acggtcccgg tagggcttga tcagcaagag gtcccagagc
     6301 gtgcccatgg cgtttatttc cagctcgtaa tagtcgtggg tgtcgccgtc cggatcgatg
     6361 aagacctcaa agtcgttatc gtgaaagatc acggcatccc gctgcgaaag cgtggcccag
     6421 atgtgtggtt cctccagctc ggcgccgata tagagaaagt ctttatccca gagcagcttg
     6481 gccctcgtcc tgaggaaggg ggccggcttc agatcaccct cgatatccac aaagtccgcg
     6541 gtccagggag cagcctgcca gtccggctca tccagccgcc cgtccatcag cagctcgccg
     6601 gtagtctgac aggcggtgta agtcaaaggc ccccaaggta gttggggagt gggaaagctg
     6661 tctgccacga gcggcagaaa catcagcact agcgtgaccg tgagcaggtg tctcatatct
     6721 tctcctaagc tgttatctta ttgtgtatca ctggctttag ccggtcgtag ggctggtctt
     6781 cagccagcac cagccaactt ggagttcaaa gcccctggcc agaaaatgcg tttatctctt
     6841 agcctgacgg ggctttggac ttacgtgcag cacatcggag agcaatatat cgcgagtgtg
     6901 ctggttgaag tccaggcggc ccgcaaactc gttgtcatgc atagttttat cctggccgct
     6961 tgaactccaa cggggtcacg caccgactaa agtcagtgct acggccggct aaagccagcg
     7021 ttacggctat tggcaccggt cttgcattac tcagtatatc caactctctg tctctcagat
     7081 gtctctgtct ctctgtccct ctgttttcag aaagggaaac agctcacgtg aagctcagtt
     7141 cgatcccgct gtagagcagc agatcctgga ttgtggagac tgccagtccc acgcaggtat
     7201 ccgcggcacc gtaatacagg taaatggggg tatcgaggtc cacttcagca tccgtcatgg
     7261 gaatggccgc ggtggggaag accacgttgg gcacctggcc gcaggtctca taaggctcgc
     7321 ggggttccag gacgttgtag ggccccctca cgatcgtctt ccagggctcc ttgaggtcca
     7381 gaaggctgag gcctgcctga tagatgcctc cacccccaaa atgagtggcc acgccgtggt
     7441 agatgtgcag ccagccgatg ggagtgcgaa tgggaggcgg cccgctgccg atcagctcgt
     7501 cccagtagtg gggctggccc ttcatcagtt cgccctcctc ctgccaggaa tagaggtccg
     7561 cggagctgta acaccctatc cggtcgccgg atcgcactcc atccggaccg gtgatttcgt
     7621 tgggacggga aagcatcaga tagcgtccat cgatcagctc tggaaataat acgccgttac
     7681 gttggtctgc gtctgacagc agcgagataa actccagatg ttcaaggtct tcactgccaa
     7741 agatgcctac ccggcaaccg gattcggtat ccatggcgca ggtgaccaga aagcgctcgc
     7801 cgatcctcac gattctgggg tcatagatgt gaaagatcct gtccgcgaag cctttgaccc
     7861 catgaaagct caccggcttg acatccgtca caaacttcag tccgtctttg gaaatggcct
     7921 tcaccagcag agtctgccgg gcccggtttt gcactctcag cagcagcgtc gtcttgccct
     7981 gccagcgaat cccgccggga ttaaagacgg agctcacgtc ccgcaggcga gaatcgatac
     8041 ccaggatatc cttccgggtc agaatgggag agaggggatg gcgtttcaaa ataaatcctc
     8101 catcctaccc atgcgctctt cgaagcctga ctcgatcttc ccggcccaaa aaagctcaga
     8161 gagcgcgtaa acccttggga acagcatctc ttccacctta tcaaaatctt ccatgtgctc
     8221 cgtccagagg ttggcctggc cacccagcag cagttccggc ctgtcaaact catagtcctc
     8281 aggtttgaga ctcatcacct tttccagcga ggtcacgcca aaggccccgg gaccgtcctc
     8341 gctgtccttc caatcaaagt agagaaactg attggggcag atcacgtaag gattgccgtt
     8401 ttgggaggct ttacgggcgg cggcgatgcc atcacctctc cagcaactga tcagaggtcc
     8461 cttgcccgga ttgccgtcca atatctcatc ccagcccatg atctccttgc ccagtttcct
     8521 caagtgcttg cgcaattccc ggatcagcca gccctgcagg gcttcttcat ccttgagctt
     8581 atggtctttg atgcgttgct ggcaatgcgg acaggccttc cagcgcgctt tgggagcctc
     8641 atcgccaccg atgtggatga taggactggg gaagagctcc gccacttcgc tcagcagctc
     8701 tttaagaaag tccagggtgg cgtctttgcc ggcgcagagg atgtcttccg agatgcccca
     8761 gacgctgagc ggctcaaact cctgggggaa acaggccagc tcgggatagg cactcagaat
     8821 ggccatggcg tggccgggca gatcgatctc cgggatcacg ttgatacagt ttagagcggc
     8881 cttgagaaga attgcctcga tctcgtcccg ggtataaaat cctccgtaac gagacccgtc
     8941 cgcctctctt ctccatgccc ccttttcggt cagcttggga aagcgtttgc tttcgatccg
     9001 ccagccctga tcatcactca gatgccagtg aaaacaattt agtttgagcg ctcccatcca
     9061 gtccagatag gtcataacct cttcggctga aaagaaatgg cggcttacat ccagatgcag
     9121 gcctcgccat ggaaagaccg atttatcgta tatggtcccg catttgatcc ggccctggtt
     9181 gtgccagatc gcttcccaca tcagctgttt taatgtttgg atgccataat agatgccatg
     9241 gctaccgggg gccatgatct ttatcacctg acgcgtgagc gtgataacgt attcgtcggt
     9301 atcctcatcc ccctcggaat cgatgctgat ttcgatgctc aggccagcca gaggtgcttg
     9361 atagcgcgct tcttttttca gtgtctccag ccagtccgtc aggctctgtt catatatgga
     9421 ttcctggatc gggtccaggc cgtcacagta aacccgcacg ttgacgggaa tccggaacga
     9481 gccacctgtc tccttcacta tcgtgggaat tgggatcatc gtttctcctt atccagcctc
     9541 agagtcttga tctcgaaagg ccggaaccgc agtatgatat gatggtcccg gagctcgaca
     9601 gcctcatcca gaggccgctc tctgaggtcg cagagacggg gctcaagata gcccttgggg
     9661 atggggaact cgagctctgc cgaagcgccc cggtactcat aaaatctcag caccaatccc
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgggccggca cctcaaagcg
     9841 cgcctcttca aagctgttgg agggtttggt gctgcgtttc agagtgccca tggcaatctc
     9901 gtaggaggcc tgatcggcat gcacatccgg gtagaaatgc gcccgcagca tcttgtgatg
     9961 ctccttccaa tccaccgcgt ggcttattct taagtaaggc gcggcgtccg aaagctcata
    10021 ggtctgcacc accgtcgaga ggccgatctg cagttcaaaa cagagccgct gcagatggct
    10081 gcccgctttc cagcttctat cctccagcag cctcacctgc gaggcctcca tgggaacggt
    10141 gtcgcggtag aaatggttga tatcccaggc gccccagttg ttgggctcgt cttcccagag
    10201 cttgaggaga ttggattccc cggccagcat ctcacggccc tccgctttga ggatgatgct
    10261 gatgattgtc cccctggggc ttagcttcac tttcagctgc tcgttttcca tcagcagctc
    10321 gtccgtcggc agggccacct gatagttgca gcgtcccgcc tcccatctga tctgcgacag
    10381 gctccagggc gccaagtcag cttttaccag cagtttatcc gggaattgca gagccggaag
    10441 ctcccgtccc tgtccatcca cggccacgct ttcgagctgc tccagcggaa actccagcca
    10501 ggcttcttta gaccaggcgc agggattgag gacggcataa accggctgcc gggcttcctc
    10561 ttcagaggca acggcggctt tgatcccgga gctgatgaaa tccttgagcc gctggtgatt
    10621 ggcctcgctg agggcatggg cgtcttcata gacctggccg atcgaggagc cggggatgat
    10681 gtcgtgaaac tgcagcagca gcgtgtcttc ccagatgtct ctgagctcac ccggataatc
    10741 attgccggat agggcagcca caaattccgc cgcgctcaag agtctctcgc tctggcggtt
    10801 gtgctttttc atcaaagcct gagtggtgta ggtaccgcgg tgaaactcca gatagagctc
    10861 gccgaagagc ttgggcagca aggccttatc caggatcttg atatggtcat aaaagtcctg
    10921 ccctttggca aagcggaagc gcggcactcc ttccagatca cgctggcgca ggccatactc
    10981 gaggtgctcg cgggtgggac caccccctcc gtctccgatc ccatagaggt tcagaaaggc
    11041 gtcgcagagc tcaccctgcg cgtatctggc ctcgctttgc aggaaggagg agggattgtt
    11101 tgagaagttg taatcgttgg tgggcagctg atgggccagg atctgggagc cgtcaatccc
    11161 ttcccacaca aagagatggt gaggaaacac gttgctctcg ttccagctca gcttttgggt
    11221 cataaaccac tggagcccgc agcccttcag cagttgcggc aggttggcgt tgaagccgaa
    11281 gcagtccggc agccacagtc ctttgggact ctggcccagt tcctcctcaa aatagcgcag
    11341 accgtaacag agctggcgga tgatcgattc tcccgagggc aggttggtgt caaattccac
    11401 ccatccggct ccctggactt cccagcgatc ctgctccacg gcagtcttaa cttcctgcca
    11461 gagatcggga tatatctctt tcatccactt ataaagctgg gcttgtgaag cgccgaagat
    11521 gta
//
LOCUS       DAOSFN010000077        11407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4793_length_11407_cov_9.418076, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000077 DAOSFN010000000
VERSION     DAOSFN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11407
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4793_length_11407_cov_9.418076"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLO35_06170"
     CDS             complement(<1..403)
                     /locus_tag="PLO35_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79290.1"
                     /translation="MKPTFIFSTMLIAMLITIAIPSGLWSQAAPLSFRMENVRSYVPG
                     PPPVTPWMFDIDDPLYNTDDGNPFTFKDDENDNSHNYAYYDFSSLFSTNYCTAENLDY
                     VQGTAGFEITFSDFQLRGFHKVNTVNPQAPWA"
     gene            complement(630..1970)
                     /locus_tag="PLO35_06175"
     CDS             complement(630..1970)
                     /locus_tag="PLO35_06175"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOH79291.1"
                     /translation="MKILILDDESRILDELGEFLGRRKHSVNAARTPSQAFAILGRET
                     FDLMVLDIRLPEMDGIEVLKRVKAEWPALEVIMISGHGDMDTVIEAMRFGACDYLRKP
                     FRQDELDVALQRSARFLGLKQEVNRLSEDNSLIAGELEKRIEKNFVGESKAIRKVLDD
                     ALHLASFAETPIIITGESGTGKEIIARIIHFASPRKEARFFPVNCAALPENLLESEFF
                     GYKKGAFTGAMQDKKGILELSQNGSVFLDELGEMPLLLQSKLLRVLEEKRFLKLGANL
                     ETSINVRFIAATNRDPEAQIQDQTLRQDLYYRLAAFRIHIPPLRERPEDIEPLLRHFA
                     SQFCSRNRIRVPHPDEDLLWELRQYPFPGNARELRNLVERALIINRDERLGLDDFPDL
                     NIAPQETDSLLERSQKQLIESALAEAGNNQSQAAKSLGISRYTLIRKMKKYGLG"
     gene            complement(1948..4110)
                     /locus_tag="PLO35_06180"
     CDS             complement(1948..4110)
                     /locus_tag="PLO35_06180"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH79292.1"
                     /translation="MSRSSAISGLWVCCLVLALLGTSLLATEQRPALQARLAEARQSG
                     SELRIRNALISLGDHYRDTSLTKAALASYLEAYGLAKKARNLNDLIYLSVGIANLKDK
                     EGQLSDALEYYTEALRWAKTGKDQAQLAKIHNNIGNLYLKIGSFSRSLKHYQDALDIK
                     RGINDPAELANALMNLSLFYLKTGSYDRSLEYQFQALKIREKGDDLSALAAVYSSISI
                     GYRHLKDYPKAFEYNRKALAIQQKLNNPAKIASLYNNLGVLYFTTGDLNQAKQAYLKS
                     YELKKSSEDKHSVLASLVNLGSLSLDLKQLAECKRWLDLAAELEPSLQSYEIIRNLAK
                     LRADYYQATGAYKDAVTQLKLYHSISDSLSSLEKARQLNELEVRYEVEEKQRNIDLLT
                     RNNLLSARELKNSVRMRNYLYLILALILVAMLLGLWRYLSVLRLSKKLYAAQADLNAM
                     NRDLEKRVETEVEIRQSQEQKALRQSRLALLGELAAGISHELNQPMQTLSFTLENIKS
                     AFQDDSLDAGYLERKLKLLFEDITRMQNVINHIRCFSKPQEEGEDSRFDLKPSIQNAV
                     EMVSDQFQRLGIALELDLPEADLRLRGNSFQFEQVILNLLTNARDAIRQQSSGQASRR
                     GIISIRAAQQGDRVIIRVSDNGCGVPTELGDQIFEIFFSTKDLEQGTGLGLAISKSTL
                     ARMKGGISYTSTPGSGTEFTIDLPIITEGESHEDIDPG"
     gene            4341..7517
                     /gene="secA"
                     /locus_tag="PLO35_06185"
     CDS             4341..7517
                     /gene="secA"
                     /locus_tag="PLO35_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOH79293.1"
                     /translation="MLEKILRKLFGDKASQDLKRYLPVVDEINAIFESLSQYDDDQLR
                     DRIVEIRQELAGKLASLRQELVELERAYREETDEAERNRIDNRIDEARKQLKQLSKDS
                     LDDYLPEVFAIVKDTCRRMVGTSFVVREHEIEWNMVPFDVQLIGGMALHDGKIAEMAT
                     GEGKTLVATLPLFLNSLLGRGAHLVTVNDYLASRDAEWMSPIFLFHGLRVGCITTGMS
                     FEERKEAYACDVTYGMNSEFGFDYLRDNMAVSPQQLVQRGFYYAIVDEVDSILIDEAR
                     TPLIISGPIAQDKNFYPEMRPMVHQVVQAQNALVQRYLSEIRDNLRENNPNPDNNLLA
                     KNLLLVKRAAPRNKAFLKLMQEGELKKLVNDTEGYYLRDKKLHELDDNLFYAVEERQN
                     SVDLCEKGRDMLSRHEKDLFVVRTLDDLHAEIDNDESITDEAERQRRKTVATNRFMDK
                     SEKLHNISQLLRAFTLFENDQEYVVIDNKVVIVDEFTGRQMPGRRFSDGLHQALEAKE
                     NVTIEAGTQTFATITLQNYFRMFERLAGMTGTAVTEESEFMEIYKLPVMAIPTNVQVT
                     RIDHEDVIYLTKNEKYQAILDEIVYWHERKKPVLVGTVSVEVSETLSRLLRRRNIAHN
                     VLNARHHQREAEIITEAGRPGAVTIATNMAGRGTDIKLGQGVVTQAIESYRNLPPSIN
                     NDNPFGVPIDGLHVIGSERHESRRIDRQLRGRAGRQGDPGTSRFYLSLEDDLMRLFGS
                     DRVAPMMMKMGLKPGDAIRHPWMTKAVERAQKRVEEHNFEIRKNLIKYDEVMNQQREV
                     IYSYRRSVLKGYSLKHEILEMIEETINAVIDQHIPEDSYPEDWNLERLAHWFRANLNI
                     GISAADLDSDHLNLEQLRNTLQEYVLQAYETKEGNVGEETLRDIERRSMLEVVDNQWR
                     DHLHEMDLLKEGVYLRAYANKDPLIEYKRESFDLFQSLITRIQEGVTRKVFTTYYLTQ
                     EQMDSLLKQANARHEDIDTFLQAKQQAAVTQNVSAPPQYSGAYDAPPEEKQRPVRVAE
                     RVGRNDPCPCGSGKKYKKCCGIHEGDEQ"
     gene            7916..10135
                     /gene="topA"
                     /locus_tag="PLO35_06190"
     CDS             7916..10135
                     /gene="topA"
                     /locus_tag="PLO35_06190"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOH79294.1"
                     /translation="MSKGLIIVESPAKASTISKFLKNQFIVKASMGHVRDLPKHDMGV
                     DIAHQFKPVYVMDDRQKKVITGLKDAIKDVPAVYLASDHDREGEAIAWHLSQALAKQL
                     EGKQVHRIVFNEITSKAINAAIEHPGIIDNAKVDAQQARRVLDRLVGYQVSPLLWKVI
                     AKDLSAGRVQSVALRLICEREAEINAFEPKEFWRLEANFWRDELPPFKASLEKWEGQK
                     AEIDTQARALELKTACETSQAVLSEIKRSSRNVEPPPPFITSTLQQEASKLLGMQSQR
                     TMAIAQQLYEGIDLKGERTGLITYMRTDSTRIADEAVTALQSLIKERFGEKLLHGSVR
                     TFKNKNSAQDAHEAIRPTDPFHTPESVAEYLSKEQLKLYTLIWQRFVATQMKSVKLAV
                     TNAQITLGPALFTASGNLITEEGFMKAYPYVNIPAGEKIHADYTKSDELEHDAIAPSQ
                     HFTTPPPRFTEASLIKELEARGIGRPSTYAAIISTIRQRDYVKLEKKIFAPTDLGNDV
                     NRFLVDKFDSIFNVKFTAAMEEKLDEVEYGKVQWPELVQSYYDSLRELIGAVDVKKEK
                     SSFVQDSGLLCDVCKEGKMLIKRSKGGEFLACDRFPACKNSKSFKRDAEGNIQIIVPT
                     QLDETCPQCGSPLMEREGRFGAFIACSNYPKCKFARPKTTGIKCPECGTGEVTQRRSK
                     QGKLFYSCNRYPDCKWISNDKPVAVQCPNCGNPYMFEKYSKARGNHKLCPKCKTIVE"
     gene            10140..>11407
                     /locus_tag="PLO35_06195"
     CDS             10140..>11407
                     /locus_tag="PLO35_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH79295.1"
                     /translation="MTAGDGIRSALQSIFSHKLRSLLTLTGIVIGVLAVVTMFSSVYA
                     LKKLIKDNMEGMGWNYSVIITAEGGERIIGPRTMRRAIRRTAQNVKTISYDDFVALRD
                     GIPHRSIYGMIESNSLHKVKGKDKFVRVRATNPEFFGNKSYPILKGRYFTDRENELLL
                     PVAVLGYYFAQDRFGDKNPVGQELALGQFRYRIVGVLDKDKLSSGNGMNFNNYERKMD
                     LEAVYVPLKYGATYFGTAGGVHMIYLQAHSEAGFATMKRTARQLLLSRHNMYPNFSFM
                     DIGDMLLTINKEIDEQMRKWNITLSVIASISLLVGGIGLFSTLLISIQERMTEIGVRK
                     SIGATDSSIFFYFLYEAVALALGGALLGILLAWLILTSIGKAIHFPLYLPIAGVGLGT
                     FFSLLVGVVSGLYPAIKAAQIDPIRAIYYM"
BASE COUNT         2701 a         3177 c         3103 g         2426 t
ORIGIN      
        1 tggcccaggg agcttgcgga tttacggtgt tgactttgtg aaagcctctc agctgaaaat
       61 ccgaaaaggt gatttcgaag ccagcggtac cctgcacgta gtccaggttt tcagccgtgc
      121 agtagttggt ggaaaacaag ctgctgaaat cgtagtaggc atagttgtgg ctgttgtcgt
      181 tttcatcgtc tttgaaggtg aaaggattgc cgtcatcggt attatagagc gggtcatcga
      241 tgtcaaacat ccagggcgtc acaggtggag gacctggcac ataggacctc acgttttcca
      301 tcctgaagct gaggggggcg gcttgagacc acaatcccga cgggatcgcg atcgtgatca
      361 acattgcgat aagcatggta ctgaagataa atgtgggttt cattttgtca cctctgtggt
      421 ttgaatttga ggatgacatg atcaggaagc gcgccaaact ggccaggctc gcgtcaaaga
      481 agtatatcta tattataatc aaatacttag agatatatca aacaggtgtg aaaaggcggc
      541 cggtccctgg taactgtgcg gtttcgatac ggattgtgat ggcaggctgt gccttatcgc
      601 acaggtatgg gcagatcgga ggggctcttt tagccgagcc cgtatttttt cattttgcgg
      661 atcagggtat agcgtgaaat gcccagggac ttggcagcct ggctctggtt gttgccagct
      721 tcggccagcg cggactcgat gagctgtttc tggctgcgtt ccagcaggga atccgtttcc
      781 tgcggggcga tattgaggtc cggaaaatca tccagaccga ggcgctcgtc ccggttgatg
      841 atcagggccc gctccaccag gttgcgaagc tcgcgcgcgt tgccaggaaa gggatattgc
      901 cgcagttccc agagcaggtc ttcatcggga tggggcactc tgatgcggtt gcgggagcag
      961 aattgagaag cgaagtgcct gagcagcggc tcgatatcct ctggtctttc cctgagcgga
     1021 gggatatgaa tgcgaaaagc ggccagacgg taatagaggt cctggcgcag agtttggtcc
     1081 tggatctgag cttcgggatc gcgattggtg gcggcgatga atctgacgtt gatgctggtc
     1141 tccagattgg cgcccagctt gaggaatctc ttctcttcca gcacgcgcaa gagcttggat
     1201 tgcagcagca gcggcatttc gcccagctcg tccagaaaca ctgatccatt ctggctgagt
     1261 tccaggatgc cttttttatc ctgcatggca cccgtgaaag cgcccttttt atagccaaag
     1321 aattcacttt ccagcaggtt ttcaggcaga gcggcgcagt tcacggggaa aaatctggcc
     1381 tccttgcggg gactggcaaa gtggatgatc ctggcgatga tctcttttcc cgtgccgctc
     1441 tcaccggtga tgataatggg agtttcggca aaggaagcga ggtggagcgc gtcgtccaat
     1501 accttgcgga tggctttgct ttctcccacg aagtttttct cgatgcgctt ctccagttcg
     1561 ccggcgatca gagaattgtc ttccgagagc cggttcacct cttgcttgag ccccaaaaac
     1621 ctggcgctgc gctgcaaggc cacatccagt tcgtcctgcc ggaagggctt gcgcagatag
     1681 tcgcaggccc caaagcgcat ggcctcgatc acggtgtcca tatcgccatg gccagagatc
     1741 atgattactt ccagagcggg ccattcagcc ttgacccttt tcagcacttc gatgccgtcc
     1801 atttccggca gccggatgtc cagcaccatc aggtcaaagg tttctctgcc caatatggca
     1861 aatgcctggg agggagtccg cgccgcgttc acgctgtgtt tgcgacggcc taaaaactcg
     1921 cccagctcgt caagaatcct gctttcatca tccaggatca atatcttcat ggctttcccc
     1981 ttctgtgatt atgggcagat cgattgtgaa ttcggtgccg gaaccaggag tggatgtata
     2041 gctgatcccg cctttcatcc tggccagagt gcttttgctg atggccaggc cgagcccggt
     2101 tccctgctcc agatctttgg tactgaagaa gatctcaaat atctggtcgc ccagttcggt
     2161 cggaacgccg cagccgttgt cgctcactct aatgatcacg cggtctcctt gttgcgctgc
     2221 ccggatgctg atgatcccac gccgggaagc ctggccggaa ctctgctgac ggatcgcgtc
     2281 cctggcattg gtgagcaggt tcaggattac ctgttcaaac tggaaggagt ttcccctgag
     2341 ccgcaagtct gcctcaggca ggtcaagttc cagagcgatg cccagacgct ggaattgatc
     2401 gctcaccatc tccacggcgt tttgaatgct gggtttgagg tcaaagcggc tgtcctcgcc
     2461 ctcttcctga ggcttggaaa aacagcggat gtgattgatc acgttttgca tgcgggtgat
     2521 gtcttcaaac aaaagcttga gcttgcgttc cagatagccg gcatcgaggc tgtcgtcctg
     2581 aaaagcgctc ttgatgtttt ccagagtgaa ggaaagggtt tgcataggct gattgagctc
     2641 gtgcgagatc ccggctgcca gctctcccag caaggccagt ctggattggc ggagggcttt
     2701 ttgctcctgg gattgcctga tttccacttc ggtctccacc cgcttttcca gatcccggtt
     2761 catggcgttg agatcagcct gggcggcata gagtttcttg ctgagcctga gcaccgaaag
     2821 atagcgccac aagcccaaaa gcatggctac caggatcagg gccagaatca gatagagata
     2881 gttgcgcatg cggacgctgt ttttcagttc ccgcgcgctc aggaggttgt tgcgggtcag
     2941 aagatctata ttgcgctgct tttcctccac ctcatagcgg acttccaatt cgttgagctg
     3001 gcgggctttt tccaggctgg aaaggctgtc gctgatgctg tggtagagct tcaattgggt
     3061 aacggcatcc ttgtacgcgc ctgtggcctg ataatagtct gctctcagtt tggccaggtt
     3121 gcgaataatc tcataggact gcagggatgg ctccagttcg gccgccaggt ccagccagcg
     3181 tttacattcc gccagctgct tcaggtccag agacaggctg cctagattca ccagcgaggc
     3241 caggacgctg tgtttatcct ccgagctttt tttgagctcg taagacttta gataagcctg
     3301 tttggcctgg ttgagatcgc cagtggtgaa atagaggaca cccaggttgt tgtagaggct
     3361 ggcgattttg gcaggattgt tgagcttttg ctggatggcg agggctttgc ggttgtattc
     3421 aaaggctttg ggatagtctt tcagatgcct gtatccaatg ctgatagagc tgtaaactgc
     3481 tgccagagcg ctgagatcgt cccccttttc cctgatcttg agggcctgga actgatactc
     3541 gagactacgg tcataactac cggttttcaa atagaagagg ctcagattca tcagagcgtt
     3601 ggccagttcc gccggatcgt tgatcccacg cttgatgtcc agggcatcct gataatgctt
     3661 gaggctgcgg ctgaacgagc cgatcttgag gtagagattg ccgatgttgt tgtgaatctt
     3721 agccaactgg gcctggtctt tgccagtttt agcccaccgc agggcttccg tgtaatactc
     3781 cagagcgtct gaaagctggc cctctttgtc tttgaggttg gcgatcccca cgctcagata
     3841 gatcaggtcg ttgaggtttc tggctttctt tgccagcccg taagcctcga gataagatgc
     3901 cagagcggct ttggtcagag atgtatcgcg ataatggtct cccaggctga taagggcatt
     3961 gcggatccgc agttctgacc cgctttgacg cgcctccgcg agccgggctt ggagcgcggg
     4021 cctctgttct gtggccagca gcgaggtccc cagcaatgct aagaccaggc agcaaaccca
     4081 taatcctgat atggcactcg atctgctcat gacatcccat ccgccgtctt ttaaagcggc
     4141 aatctgacgg ccatggcact tgttgtcaat gaaaaagcgg atcagggacc cgaaaccgcg
     4201 tcaattcaga aagcagcccc cccgccaaag caaagtagtt gacacaatca ccctgccaat
     4261 cagagtggca accggtcacc ctcccaggca gactttttat gaacacagag cgcttgggaa
     4321 gagccgataa ggaacacatt atgttggaaa agatattaag aaagctgttc ggagataaag
     4381 ccagccagga cctcaagcgc taccttccgg ttgtggatga gatcaacgcc atctttgaat
     4441 ccctcagcca gtatgacgac gatcaactgc gtgacaggat agtggaaatc cgtcaggagc
     4501 ttgccggaaa actggcttct ctgcgccagg aactggtaga actggaacgc gcctatcggg
     4561 aagagactga cgaggctgaa cgcaaccgca tcgacaaccg catcgacgaa gcccgcaaac
     4621 agctcaagca gctcagcaag gacagcctcg acgactatct gccggaagtg tttgccatcg
     4681 taaaagatac ctgccgcaga atggtgggta ccagctttgt ggtgcgggag cacgagatcg
     4741 agtggaacat ggtgcccttc gacgttcagc tcattggcgg tatggccctg cacgacggca
     4801 agatcgctga aatggccacc ggtgagggta agaccctcgt ggccaccctg ccgctgtttt
     4861 taaacagctt gctgggccgc ggagcccatc tggtgacggt gaacgactat ctggccagcc
     4921 gcgacgccga atggatgagc cccatcttcc tgttccacgg tctgagagtg ggatgcatca
     4981 ccaccggcat gagctttgag gagcgcaaag aggcctacgc ctgcgacgtc acctatggca
     5041 tgaacagcga atttggcttt gactatctca gagacaacat ggccgtctca cctcagcaac
     5101 tggtgcagcg cggcttctat tacgccatcg tggacgaggt ggacagcatc ctcatcgacg
     5161 aggcccgaac tcctctcatc atcagcggcc ccatcgctca ggacaaaaac ttctatccgg
     5221 aaatgcgccc catggtgcat caggtggtgc aagcccaaaa cgctctggtg caacgctatc
     5281 tgagtgagat cagagataat ctgagggaga ataatcccaa cccggacaac aacctcctgg
     5341 ccaaaaacct cctgctggtc aagcgggccg cgccccgcaa caaagctttt ctgaagctca
     5401 tgcaggaagg cgagctcaag aaactggtca acgacaccga aggttattat ctgcgggaca
     5461 agaaactgca tgagctggac gacaacctct tttacgccgt ggaagagcgc cagaacagcg
     5521 tggacctctg tgaaaagggc cgcgatatgc tgtcccggca tgaaaaagac ctctttgtgg
     5581 tgcgcaccct ggacgatctg catgccgaga tcgacaacga cgaaagcatc acagacgagg
     5641 cggaacgcca gcgccgcaag acagtggcca ccaaccgttt tatggacaag agcgaaaagc
     5701 tgcacaacat cagccagctc ctgcgcgcct tcaccctgtt tgaaaatgat caggaatatg
     5761 tggtgatcga caacaaggtg gtgatcgtgg atgaatttac cggacgccag atgccaggcc
     5821 gccgcttttc ggatggactg catcaggccc tggaagccaa ggaaaacgtc accatcgaag
     5881 ccggcaccca gacctttgcc accatcaccc tgcaaaacta ctttcgcatg tttgaacgcc
     5941 tggccggtat gactggtacc gccgtcaccg aagaatccga attcatggag atctacaagc
     6001 tgcccgtgat ggccatcccc accaatgtcc aggtgacccg tatcgaccac gaggacgtga
     6061 tctacctcac caaaaatgaa aagtatcagg ccatcctgga cgagatcgtc tattggcatg
     6121 aacgcaagaa acccgtgctg gtcggcaccg tgagtgtgga ggtgtccgag accctcagcc
     6181 gcctcctcag acgcagaaac atcgctcaca acgtgctcaa cgcccgtcac catcaacgtg
     6241 aggccgagat catcaccgaa gccggacgtc ccggggccgt gaccattgcc accaacatgg
     6301 ccggccgcgg aacagacatc aagctgggcc agggcgttgt gacccaggcg attgaaagct
     6361 accgcaatct gcccccctcc atcaacaacg acaatccctt tggcgtaccc atcgatggac
     6421 tgcatgtgat cggtagcgag cgtcacgaga gccgtcgcat cgaccgccag ctcagaggcc
     6481 gggccggacg tcagggcgat cctggcacct ccagattcta tctgtctctg gaagatgacc
     6541 ttatgaggct gtttggcagc gacagggtag cccccatgat gatgaagatg ggcctcaaac
     6601 ctggagacgc catccgccat ccctggatga ccaaagccgt ggagcgggcc cagaaacgcg
     6661 tggaagagca caactttgaa atccgcaaaa acctcatcaa atatgacgag gtgatgaacc
     6721 agcagcgcga ggtgatctat tcctaccgcc gcagcgtgct caaaggctac agcctcaaac
     6781 acgagattct ggagatgatc gaggagacaa tcaatgccgt gatcgatcag cacatccctg
     6841 aagacagcta tcccgaagac tggaatctgg aacgcctggc ccactggttt cgggccaacc
     6901 tcaatatcgg catcagcgcc gcggacctgg attccgatca cctcaacctg gaacaactgc
     6961 gcaacaccct gcaggagtac gtcttgcagg cctacgaaac caaagagggc aacgtgggcg
     7021 aagaaaccct gcgtgatatc gaacgccgca gcatgctgga agtagtggac aaccagtggc
     7081 gggaccatct gcatgagatg gacctgctca aggaaggtgt gtacctgcgc gcctatgcca
     7141 acaaagatcc cctcatagaa tacaaacggg agagcttcga cctcttccag agcctcatca
     7201 cccgcatcca ggaaggcgta acccgcaagg tctttaccac ctactacctc acccaggagc
     7261 agatggacag cctcctcaaa caggccaatg cccgtcatga ggatatcgac accttcctgc
     7321 aggccaaaca gcaggccgcc gtgacccaga acgtgagcgc tccgccccag tacagtggag
     7381 cttacgacgc ccctccggaa gaaaagcagc gccccgtgcg cgtagccgaa agagtgggcc
     7441 gcaacgatcc ctgtccctgc ggcagtggca agaaatacaa gaaatgctgc ggcatccatg
     7501 aaggcgatga gcaatgagca tccaggatgg agtgagcctt ttgacgaatt ggaagaaagc
     7561 agagaaagca gaggatgtct tgtgtatctt ggagttcaaa gcgccatacg ctgacccggc
     7621 attgtatcaa gatttcgggc gctttggact tcaaacagtg catcggagcg cgtgtattgt
     7681 caaggattac cctggggcgt atcttcctgc ggtgggctgg ttgaagtcca aacggcccgc
     7741 aaactcgctg ccatgtaatc cattatcccg gccgcttgaa ctccaactgg accatctgta
     7801 ttctccgctt tctctgaaga atgatgagaa cataaacacc atcaatagca tcaattcccg
     7861 ccttcgcggg acggacaatt caatcgccaa ccgcaaatat gtataaggat agagcatgtc
     7921 aaaaggtctg atcatagtgg aatctccggc caaagccagc acgatttcca agttcctcaa
     7981 aaaccagttt atcgtcaaag cctccatggg ccatgtacgc gatcttccca aacatgatat
     8041 gggcgtggat atcgcgcatc aatttaaacc cgtctatgtg atggacgatc gtcagaagaa
     8101 agtgatcacc ggcctcaagg atgccatcaa agacgtcccg gccgtctatc tggccagcga
     8161 tcatgaccgt gagggagagg ccattgcctg gcatctttcc caggccctcg ccaagcaatt
     8221 ggaaggcaaa caggtgcacc ggatcgtttt caacgagatc acttccaagg ccatcaacgc
     8281 cgcgatcgaa caccccggca ttatcgacaa cgccaaggtg gacgctcagc aggccagacg
     8341 cgtcctggac cgcctggtgg gctatcaggt gagccccctt ttgtggaaag tgattgccaa
     8401 agatctcagc gccggcaggg tacaatctgt ggccctgcgc ctgatctgtg aacgtgaagc
     8461 cgagatcaac gcctttgagc ccaaagagtt ctggcgtctg gaagccaatt tctggcgcga
     8521 cgagctgcct cccttcaagg ccagcctgga gaaatgggaa ggccagaaag ccgagatcga
     8581 cacccaggcc agagcgctgg aattgaagac tgcctgcgag acaagccagg ccgtcctctc
     8641 tgagatcaaa cgctccagcc gcaacgtcga accgccccct ccctttatca caagcactct
     8701 gcagcaggaa gcctcaaagc tcctgggaat gcagtctcag aggactatgg ccattgccca
     8761 gcagctctat gaaggcatcg acctcaaggg tgagcgcacc ggtctgatca cctatatgcg
     8821 taccgacagc acccgcatcg ccgatgaggc tgtcacggcg ctccaatcct tgatcaaaga
     8881 gcgctttggc gaaaagctgc tgcatggctc tgtccggaca ttcaaaaaca aaaacagcgc
     8941 tcaggatgct cacgaagcga tccgccccac cgatcctttt cacactcctg agagcgtggc
     9001 ggaatacctc agcaaagaac agctcaagct ctacactctg atctggcaac gctttgtggc
     9061 tacccaaatg aagagtgtga agctggccgt caccaatgcc cagatcactt tgggaccggc
     9121 cctgttcacg gccagcggca atctgatcac cgaggaaggc ttcatgaaag cctatcctta
     9181 tgtcaatatc cctgccggag agaagatcca cgccgattac accaagtctg acgagctgga
     9241 gcatgacgcc atcgctccca gccagcattt taccactccc ccgccccgct tcacggaagc
     9301 ctctctgatc aaagaacttg aggcccgcgg catcggacgc ccctccacct atgccgctat
     9361 catcagcacc atccgtcaac gcgattacgt gaaactggag aaaaagatct ttgcgcccac
     9421 cgacctcggc aacgacgtca accgcttcct ggtggacaag tttgattcca tcttcaacgt
     9481 caaattcacc gcagccatgg aagagaaact ggatgaagtg gaatatggca aagtccagtg
     9541 gccggaattg gtgcagagct attacgacag cctgcgtgaa ctgatcggcg ctgtggacgt
     9601 caaaaaggaa aagagctcct tcgtgcagga cagcggcctg ctctgtgacg tgtgtaaaga
     9661 aggcaagatg ctgatcaaga ggtccaaagg cggggaattc ctggcctgcg accgctttcc
     9721 ggcttgcaaa aacagcaaga gcttcaaacg cgacgcggaa ggtaatatcc agatcatcgt
     9781 gcccacccag ctggacgaaa cctgtcccca gtgtggttca cctctgatgg agcgggaagg
     9841 acgctttgga gcttttatcg cctgctccaa ctaccccaag tgcaaatttg cccggcccaa
     9901 gaccaccggg atcaaatgcc ccgaatgcgg caccggagaa gtaacccagc gccgcagcaa
     9961 acaaggcaag ctcttctatt cctgcaaccg ctatccggat tgcaaatgga tcagcaacga
    10021 caaacccgtg gccgttcagt gccccaactg cggcaacccc tatatgtttg agaaatattc
    10081 caaggcgcgg ggcaaccaca agctctgccc caagtgcaag accatagtgg aatagaaaga
    10141 tgaccgccgg ggacggaatc cgcagcgcgc tgcagagcat tttcagccac aagctgcgtt
    10201 ctctgctcac cctcaccggc atcgtgatcg gcgtcctggc cgtcgtgacg atgttttcca
    10261 gcgtctatgc cctcaaaaag ctgatcaagg acaacatgga aggcatggga tggaattact
    10321 ccgtgatcat caccgccgag gggggagaaa ggattatcgg accccgcacc atgagacggg
    10381 ctatcaggcg aacggcccaa aacgtcaaga ccatcagcta tgatgacttt gtagccctgc
    10441 gggacggcat tccccaccgc agcatctatg gcatgatcga aagcaatagt cttcacaaag
    10501 tgaagggcaa agacaagttt gtgcgcgtcc gggccaccaa tccggagttc tttggcaaca
    10561 agagctatcc cattctcaag ggacgttact ttacagaccg ggaaaacgaa ctgctgctgc
    10621 cggtggccgt attgggctat tactttgccc aggataggtt tggcgacaaa aaccccgttg
    10681 gccaggagct cgcgctgggc caatttcgct atcgcatcgt gggagtgctg gataaagata
    10741 agctctccag cggaaacggg atgaatttca acaactacga gcgcaagatg gacctcgagg
    10801 ccgtgtatgt gcccctcaag tacggcgcca cttattttgg taccgccggg ggagtgcaca
    10861 tgatctatct gcaggcccac tccgaagccg ggttcgccac catgaagagg accgcccgtc
    10921 agctcctgct ttccaggcat aacatgtatc ccaatttcag ctttatggat atcggagaca
    10981 tgctgctcac tatcaataaa gaaatcgacg agcagatgcg caaatggaac atcaccctct
    11041 cggtcatcgc gtccatctca ttgctggtgg gaggtatagg cctcttttcc accctcctga
    11101 tctctatcca ggaacgcatg accgagatcg gagtgcgcaa aagcatcggc gccacggaca
    11161 gcagcatctt tttctatttc ctctacgaag cggtggcttt ggccctgggc ggagcgctgt
    11221 tgggcatcct actggcctgg ctgattctca cttcgatcgg caaagcgatt cactttccgc
    11281 tctatcttcc tatcgcgggg gtgggattgg gcaccttctt ctctctgctg gtgggagtgg
    11341 tctctggcct ctacccagcc atcaaagccg cgcagataga ccctatcagg gcaatctatt
    11401 atatgga
//
LOCUS       DAOSFN010000078        11401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4798_length_11401_cov_9.318967, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000078 DAOSFN010000000
VERSION     DAOSFN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4798_length_11401_cov_9.318967"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLO35_06200"
     CDS             complement(<1..243)
                     /locus_tag="PLO35_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79296.1"
                     /translation="MNIRNAMPLRLARRSSIGHDKGIHDNEIAGRLDFNQHTCGQNAS
                     DVLLEVQSPLKQGDKRLFCPGALNFVELTNPLPAMKT"
     gene            complement(390..1316)
                     /locus_tag="PLO35_06205"
     CDS             complement(390..1316)
                     /locus_tag="PLO35_06205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOH79297.1"
                     /translation="MPELPIAHVYSKIFESSVVGIGITDLAGKYVMVNPAWCEFMGYS
                     EEEAKNLNVRDTTMPEERRQSDNSLKKIIDGELSSIRKTRRYLRKDGSCFWADLHVSA
                     LTDEQGQVIGVIGMLINIDRQVKAENYQKDLNRQLSKMARHDTLTGLYNRRAMDSIMI
                     REHKRAHRYKRGFAIAIGDVDNFKMINDTYGHDCGDMVLKHMAETFLEWIRDTDTVGR
                     WGGEEFLFVFSETTCEGARIVAERIRSSLDNKLFRCGDHEFHLSLTIGFSYHSEQVSV
                     EQMIAEADKALYRGKGNGKNQVVCHQDVCGSQ"
     gene            complement(1484..1993)
                     /locus_tag="PLO35_06210"
     CDS             complement(1484..1993)
                     /locus_tag="PLO35_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOH79298.1"
                     /translation="MISKKLEKAINEQINRELFSEYLYISMQAWFAGENLDGMATWMD
                     AQGKEERFHAMKFFNYLLDRGGKVKLMALDKPEVDFKNPLNAFREAYKHEQFISKSIN
                     QLMDLAIAENDHAAKSFLQWYVDEQVEEEATAEKIVRQLEMVSDNMAGIFMMNAELGK
                     RVYTPPVSE"
     gene            complement(1995..3185)
                     /locus_tag="PLO35_06215"
     CDS             complement(1995..3185)
                     /locus_tag="PLO35_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOH79299.1"
                     /translation="MKAIQLKKDIWWVGGIDWDLRNFHGYLTQRGSTYNAYLIIDEKI
                     TLIDNVKSYLFDEMIARISDVIDPARIDILIQNHVEMDHSGGMPAMMKLLPNATIYTN
                     ANCIKGLKAHYKQDWLFHEIKSGDSINIGSRDLSFLTTPMVHWPDNQFTYCPQDKLLF
                     SNDAFGQHIASSERLAKDFPFSIVMEEAKKYYANIVLPYSKQVQKVLEAAAPLQIEMI
                     APSHGLVWTDHIPQILEAYKDWAFNVANPKQALVIYDTMWKSTQMMAFAIADAFENLG
                     IKAKMLNLQTNHISDIMTDVMDAHYICVGSPTLNSSILPTVAAFLYYLKGLSPKDRVG
                     LAFGSYGWGGQSIPILHQLLGDPKECGFDMLDPIKVQYVPDASTLTNITKELEQKIKT
                     KMEN"
     gene            complement(3344..4156)
                     /gene="mutM"
                     /locus_tag="PLO35_06220"
     CDS             complement(3344..4156)
                     /gene="mutM"
                     /locus_tag="PLO35_06220"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOH79300.1"
                     /translation="MPELPEVQTVLNGVVASIGKHKIRSLECFYPGTVIRSPQLSKEP
                     FPARLINHRRRGKYIILDLEGGHSLIIHLRMTGKLVWDKDSGPDHIHERACFHLEGSR
                     RMRFIDPRTFGKIILCPTSEVDTYLPALGAEPLEAEFDAQYLQSRLKGRKMPIKNALL
                     DQKVVAGLGNIYVCEVLYRAKLHPDTPCGELKLPSLKRIVQHTKTILIEALANNGTSI
                     SDFRRVDDKQGGFQEFLRVYQKETCPLGHPIQRTKHAGRGTFFCAVCQDRKS"
     gene            complement(4220..5185)
                     /gene="trxB"
                     /locus_tag="PLO35_06225"
     CDS             complement(4220..5185)
                     /gene="trxB"
                     /locus_tag="PLO35_06225"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOH79301.1"
                     /translation="MLDFNVSLSATAEPDKLWDLIIIGGGPAGLTAGLYASRYKLEVL
                     LLEKAVVSGGQLTSTQWVENYPGFPEPVLGTKLAADMEAQARLFGLRIISETVKSVSL
                     TGIVKEIVTDYATYRAKTVLIATGSSPRRLGLPHEAEFSGRGVSYCATCDGPFYPDKT
                     VAVAGGGNSAFEESLFIAKYAAKIYLIHRRDSFSADPILQERVKAEPKIELITNTQVT
                     ALNFDTDTQRSISLINTQSGAESLLEVDGIFIFVGNLPNTTLFEGQLELEQGYIVTDR
                     SYRTSLEGVWAVGDVQAGALRQVATAVGDGAGAAHKINKYIIDNA"
     gene            complement(5187..5825)
                     /locus_tag="PLO35_06230"
     CDS             complement(5187..5825)
                     /locus_tag="PLO35_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOH79302.1"
                     /translation="MPMLDEKVMTEVKKALNAMRKPVTLRLYTQAFECGYCKETHNLL
                     SELVQANPLLKLETKQFETDKEEAAQDNIDKIPAIAVIGEKDYGIRFYGIPAGYEFSS
                     LLNAILMVSTGIVKLTDETKAFVANLQTPLHLQVFVTPTCPYCPQAVILAHQLAMASD
                     QITADMVEISEFPHLAQKYQVQGVPRTVINEKWSLDGAAPEAMLVAKAKEAL"
     gene            complement(6007..6801)
                     /locus_tag="PLO35_06235"
     CDS             complement(6007..6801)
                     /locus_tag="PLO35_06235"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOH79303.1"
                     /translation="MIRSGSKPLYVVLIILGILVIGMAALMAAQKLGLDIGWLAFKRP
                     TPASRTTVMIADSLTAFPDTLELQKAVEPGQKQEPKAVAAVQDTLKKKPAKPQKKSSF
                     NPQDSLWVAYTRDDSTHIESPGEGYLIRISKGKRQLTLFKDGVPQKVYSVGVGKNQND
                     KSKKADNATPEGSFLIDSIHNSRDWKYNGKKVYGPWFLRLNTSSGAFSGSSWTGIGIH
                     GTSNEKSIGNFVSKGCIRMYNKDITELKNTVAAAVDSSTVRVLILP"
     gene            complement(7115..11383)
                     /locus_tag="PLO35_06240"
     CDS             complement(7115..11383)
                     /locus_tag="PLO35_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH79304.1"
                     /translation="MNTSTPGNAGITAALWKSVDGFANINAATDNNIWYAGTPGPRNL
                     ICRTPSVYYETLDDYKAAAVSFDQSSFTEAVPFVDGVGPDYDIHIPIGAATIAESNAT
                     PIDGIVTDIDLETRNASTPDIGADEGNFVVPANAPAPAVVVSPQMNEYAVSINPSLTW
                     YASGAGGAPTCYLVYFGSNNPPSDIADGIDIGNVLSYVSPVTLNYLSTYYWKIVPYND
                     NGSTDPDDCPVWQFSTHQVPLTGNWVIGSSGFYPDFTHAIRYLNASGVGTGGVTFKAL
                     ATEVFAENPPPLVVSGTAADPIVFTSTDTLAVNPLITPSEGAGSYGIKLEGADFVTFD
                     HIDIANQPGQSGLVYGYWLEAQTDDGCTDNSIQNCSITMARATDTFGIRSDSASGRIN
                     HRNRYLRNHISGPKNGIYLYDSSAAQNLVLSGNQISSVSTYGIYIRYTNNLEVSYNQI
                     GMAATNTGNFTGINCYNESGTGQIHHNQIIGGSTTGTVNGIYHHYDKFNIYSNQISGI
                     NASSHIYGIRNYYGGSNGLVYDNLVADLASSGTSDVAGIIMNSSVVYQNTVSGLSSNG
                     NVYGMNISNGSVYRNDIHNLQTGGSSSFVNGLYTNGTVTAHNNMICDLRAPSSNSVPQ
                     VRGIYVYAGTSNLYYNSVLITSSGTGSHSSAALYSNNPTLLNPQNNIFSNLSTPGASG
                     KAVAFWNAQPDFDAVSDASNNNIWYAGIPSAQNLICYYGTSSCQTLELYKAANAGKDQ
                     NSFSEDAPFLSKLSPFDLHLDPLVETYAEGNALVIASVGIDYDGQTRNASRPDIGADE
                     GAFLEVQLPPALPVYLSPANGAIDLALNTSITWAAGPGGGNPDYYIVYFGETQTPPQI
                     ATNVTATHYAPSLLPERTYYWKVDAVNDLGTAAGVDIWSFDTRADDTIMEFPYTESFE
                     DGNTNGSTTINRWSQALGSGSYYWTANTSTNYNREPKTGSYNLTLRDGGDAWIFRPIY
                     LTTGQMYELELWARQYTASGAQAYLQVRYGPAPSIAGMTGTIINIQEFVNGDYQRGTG
                     TFTPPSTGIWYLGIHGVCTNYINYLSLDDISINFYEPYPSFSIDPSSWNYGMINVSQS
                     SDAKAFVISNPGDADLVILQGDVYLEGNHQDDFVLTDPAEEIVVAAGESYTIYVSFAP
                     QELGVRTANLMIVDNIENRAIHQIPLTGRGIGPLQPPCVEDFEDGWVDWITVNTDQPN
                     KWELGSAAPYRNSYSAYISSNYGATHSYNPNSGSYSHFYHDVSFPASMAGMKLRFQYK
                     CAGEAGFDYLTVHITDTSFTPVAGSSFSDGQIGTAYHSSPDWQYVSIELDPAMAGQLK
                     RLIFSWRNDSGGGTQPPAAIDNIRIYSSWPYLDPQEAPQDLQISSSGTNVILSWEKVT
                     GANDYIIEDADMYDGLFTPVGRGFNSFTVPGSHPRRFFRVRATD"
BASE COUNT         2405 a         2804 c         3304 g         2888 t
ORIGIN      
        1 cgttttcatt gccggtagcg gattagtcaa ttcaacgaag ttcaaagccc ctggacagaa
       61 taggcgttta tctccttgct tgagggggct ttggacttca agcagtacat cggaggcatt
      121 ttgtccgcaa gtgtgctggt tgaagtccaa gcggcccgca atctcgttgt catgtatacc
      181 tttatcgtgg ccgattgaac tccgacgggc caatctcagc ggcattgcgt tcctgatatt
      241 cacttgcccg ccacaccagc ctcacggctg ccccacctat gcctgggcaa aggtgaggca
      301 aaggagatgt aaaggagggg caaaggaggc taagcgggaa ggcaatgggt gtaaaccagg
      361 tttacacggg attgtcgacc tgattgaatc tactgtgaac cgcagacgtc ctgatggcaa
      421 accacctgat tcttgccgtt gcccttgccc ctgtaaagtg ctttatcggc ttcggctatc
      481 atctgttcca ctgaaacctg ctcactgtga tagctgaacc cgatcgtgag gctgaggtga
      541 aattcgtggt ccccgcagcg gaagagcttg ttgtccaggc tggagcgaat acgttccgcc
      601 acgattctgg cgccttcgca agtggtttcg gaaaacacga agagaaactc ctctccgccc
      661 cagcgtccca cggtatcagt gtcacggatc cactctaaaa acgtttcggc catgtgtttg
      721 aggaccatat ccccgcagtc gtgaccatag gtatcattga tcattttaaa gttgtccacg
      781 tctccgatag caatggcaaa gcctcgtttg taacggtggg cgcgtttatg ttcgcggatc
      841 atgatgctgt ccatggccct gcggttatag aggccggtga gggtgtcgtg gcgggccatt
      901 ttgctgagct ggcggttcag atctttctga tagttttccg ctttgacttg ccggtcgatg
      961 ttgatcagca tgccgattac cccgatcacc tgtccctgct catcggtgag agctgagacg
     1021 tgcagatcgg cccaaaagca agagccgtcc ttgcgaagat agcgcctggt tttgcggatg
     1081 ctggacagtt cgccatcgat gatctttttg agggagttgt cgctttgccg gcgttcttct
     1141 ggcatggtgg tgtctctcac attgaggttt tttgcttctt cctcagaata gcccatgaac
     1201 tcacaccagg cgggattgac catcacatac ttgccggcga gatcggtgat gccgatgccc
     1261 acgacggagc tttcaaaaat cttggaatac acatgcgcga taggtaactc aggcataagc
     1321 tcttcctttt ggtttataag ttgttgccgt aaaaaacata aaacaaatgg ccgcaacaaa
     1381 agcggcaaac atacctcggc ccggataggg gccgtttaaa acaagataaa gcaacagcgc
     1441 gggaaagcaa cactttcccg cgcacatcat tatatggatc ttattattcg cttaccggcg
     1501 gcgtgtagac acgcttgccc agctcggcgt tcatcatgaa gatgccggcc atgttgtcgc
     1561 tcaccatctc aagctggcga acgatctttt ccgcggtggc ctcttcttcc acctgttcgt
     1621 ccacatacca ctgcaggaag cttttggcgg catggtcgtt ttcggcgatg gcgaggtcca
     1681 tcagctggtt gatgctcttg gaaatgaact gctcgtgttt ataggcttcg cggaaggcgt
     1741 ttagcgggtt tttgaaatcc acctccggtt tgtccagggc catgagtttc accttcccgc
     1801 cgcgatccag gaggtagttg aaaaatttca tggcgtggaa gcgttcttct ttgccctggg
     1861 cgtccatcca ggtggccatg ccatccagat tttcacccgc gaaccatgct tgcatggata
     1921 tatagaggta ttcactgaac agctcgcggt tgatctgttc gttgatggct ttttccagtt
     1981 ttttgctgat catgttagtt ctccattttg gttttaatct tttgttcaag ttctttggtg
     2041 atgtttgtga gggtggaggc gtccggaaca tactgcactt tgatcgggtc cagcatgtca
     2101 aagccgcatt ctttggggtc tcccagcagt tgatgcagga tgggaatgct ctgtccgccc
     2161 cagccatagg acccgaaggc caggcccact ctgtctttgg gggagaggcc tttgagataa
     2221 tagaggaagg ccgccacggt aggtaaaata gagctgttga gggtgggtga gcccacgcag
     2281 atgtaatggg cgtccatcac atccgtcatg atgtctgaga tgtgattggt ctgaaggttg
     2341 agcatcttgg ctttgatgcc caggttttca aaggcgtccg cgatggcgaa ggccatcatc
     2401 tgggtggatt tccacatcgt gtcgtagatt accagggcct gtttggggtt ggccacgttg
     2461 aaggcccagt ctttataggc ttccagaatc tgggggatat gatcggtcca gaccagtccg
     2521 tggctgggag cgatcatctc gatctggagg ggggccgcgg cttccagcac cttctgcacc
     2581 tgcttggaat agggcagcac gatgttggcg taatactttt tggcctcctc catcacgatg
     2641 ctgaagggaa agtcctttgc caggcgttcg ctggacgcga tatgctggcc aaaggcatcg
     2701 ttggaaaaca ggagtttgtc ctgagggcaa taggtgaatt ggttatcggg ccagtgcacc
     2761 atcggagtgg tgaggaagct gagatcgcgg gagccgatgt tgatgctgtc gccgctcttg
     2821 atctcgtgaa aaagccagtc ctgcttgtag tgggccttga ggcctttgat gcagttggcg
     2881 ttggtataga tggtggcgtt gggcagcagt ttcatcatgg ccggcatgcc gcctgaatgg
     2941 tccatttcca catggttttg gatgaggatg tcgatcctgg cggggtcgat gacgtcactg
     3001 atgcgcgcga tcatttcatc gaagagatag cttttgacgt tgtcgatcag cgtgatcttt
     3061 tcatcgatga tcaggtaggc gttgtaggtg gaaccccgtt gagtgaggta gccgtgaaag
     3121 ttgcgcaggt cccagtcgat gccccctacc caccagatat cttttttcag ctggattgct
     3181 ttcatgatct tagctccata tttttcttat aatttattag taaattccta cctatagtat
     3241 aggtgaccgc ttctgatcaa gcaagggaaa agaggaatgg gttgagacgg ttgaaaaggt
     3301 tgcgaggttt caggatgaca accatatcaa cctaaagctg atcctagctc tttctatcct
     3361 ggcagactgc gcagaaaaag gtcccccggc ccgcgtgctt ggtcctctga atgggatggc
     3421 ccagcgggca ggtctccttc tgatacactc tcagaaactc ctgaaatccg ccttgcttgt
     3481 catccacgcg cctgaagtcc gaaatcgagg taccgttgtt ggccagcgct tcgatcagga
     3541 tggtcttggt gtgttgcact atcctcttga gagaaggcag tttgagctca ccacagggag
     3601 tgtctggatg cagctttgcc cggtagagga cctcgcagac atagatgttt cccagtccgg
     3661 ccaccacctt ttggtccagc agggcgttct tgatgggcat cttgcggcct ttgagcctgc
     3721 tctgcaggta ttgggcgtca aactcggctt ccaaaggttc cgctcccagc gcgggcagat
     3781 aagtgtccac ttcagaagtg gggcagagga tgatcttgcc aaaggtgcgg ggatcgataa
     3841 aacgcatcct gcggctgcct tccagatgaa aacaagcccg ttcgtggatg tgatcgggac
     3901 cgctgtcctt atcccacacc agcttgccag tcatccgcag atggataatc aggctgtggc
     3961 ctccttccag atcgaggatg atgtatttgc ctctgcggcg atgatttatc agcctggcag
     4021 ggaagggctc tttgctaagc tggggagagc ggatcaccgt gccgggatag aaacactcca
     4081 gagacctgat tttgtgcttg ccgatgctgg ccacgacgcc attgaggacg gtctgtactt
     4141 ccggaagttc tggcatgatc tatattcctt tgttatcatc tgtcaatctc gttgcggccg
     4201 gagcggaatc cggagcatct caagcattgt cgatgatgta tttgttgatt ttatgagccg
     4261 ccccggcccc atctccaacc gctgtggcta cctgacgcaa ggctcccgcc tggacgtctc
     4321 ccacagccca aacgccttcc aggctggtgc ggtaggagcg gtccgtgacg atgtatcctt
     4381 gctcgagctc gagctgtcct tcaaagagag tggtgttggg caggttgccc acaaagatga
     4441 agattccgtc aacctccagc aggctttccg ctccgctctg ggtattgatc agactgatgg
     4501 agcgctgggt atcggtgtca aagttgaggg ccgtcacctg agtgttggtg ataagctcga
     4561 ttttgggctc ggccttcacc ctttcctgca ggatgggatc agcgctaaag ctgtctctgc
     4621 ggtggatgag atagatcttg gcggcatatt tggcgatgaa gagcgattct tcaaaagcgg
     4681 aattgccacc acctgccacg gccacggttt tatccggata aaagggaccg tcacaggtgg
     4741 cgcagtaaga tactccccgg ccactgaatt cagcctcatg gggtaatcca agcctgcggg
     4801 gtgaagaccc cgtggcgatc aggacggttt tggccctgta ggtggcatag tctgtcacga
     4861 tctcttttac gattccggtc agggatacgc ttttgaccgt ctcggagatg atcctgaggc
     4921 caaagagcct ggcctgggct tccatatcgg ccgccagttt ggtacccagc acgggctctg
     4981 gaaagccagg atagttttcc acccactggg tggaggtgag ctgtcctccc gaaaccacgg
     5041 ctttttccaa cagcaatact tccagcttgt aacgggaagc atagagcccg gcagtcaaac
     5101 cggcaggacc tcctccgatg atgatgaggt cccagagctt gtcaggttcc gcggtggcag
     5161 acagactgac gttgaaatcc aacatcttag agcgcctctt tggccttggc caccaacatc
     5221 gcttccgggg ccgcgccatc cagggaccac ttttcgttga tcacagtgcg gggtacaccc
     5281 tgcacctgat atttctgggc cagatgggga aattcactga tctccaccat gtctgcagtt
     5341 atctgatctg atgccatggc aagctgatgg gccagaatca cggcctgagg gcaataggga
     5401 caggtgggag tcacaaaaac ttgcaaatga aggggggtct gcagattggc gacaaatgct
     5461 ttggtctcgt ccgtgagctt gacgataccg gtggaaacca tcaggatggc gttgagcagg
     5521 gaagagaatt catagcccgc ggggatgccg taaaagcgga tgccataatc cttttcgccg
     5581 atcacggcaa tggcgggaat cttgtctatg ttgtcctggg cagcttcttc cttatcagtc
     5641 tcaaactgct ttgtttccag cttcaataac ggatttgcct gcaccagctc tgacaaaaga
     5701 ttgtgggttt ccttgcagta accgcattca aaggcctgag tatataatct gagcgtaacg
     5761 ggcttgcgca tggcgttgag cgctttcttg acctcggtca tgactttttc atccaacatt
     5821 ggcatgggtt gcctcctttg tttattctgg agatagaata agtaaaaatg aaccggatgt
     5881 caaataccaa tttgagattt tctgaatgag agaggattta gatacgggta gccacttcgg
     5941 agggggggta ggattatggg gttttagggt tttaaagttg tgtcagcaac tcgcagaact
     6001 cacccttcag ggcaggatca gcactctcac ggtggagctg tccacggccg cggccacggt
     6061 gtttttgagc tcggtgatat ctttgttgta catgcggata cagcccttgg acacaaagtt
     6121 gccgatgctc ttttcgttgc tggtgccgtg aatgcctatg ccggtccagc tgcttccgga
     6181 aaaagctccg ctggaggtgt tgagccgcaa aaaccaggga ccatatacct tttttccgtt
     6241 gtatttccag tccctggaat tgtggatgct gtcgatcagg aagctgcctt ccggagtagc
     6301 gttgtctgcc tttttgctct tatcattctg gttttttccc actcccaccg aatagacttt
     6361 ctgagggact ccgtctttga acagagtgag ctggcgcttg cctttggaaa tcctgatcag
     6421 ataaccttct ccgggggatt cgatgtgggt gctgtcatcc cgggtgtagg ccacccagag
     6481 agagtcttgg ggattaaagc tgctcttttt ctgcggtttg gcgggtttct ttttgagcgt
     6541 atcctgcacc gcggctacgg ctttgggctc ttgtttttgc ccgggctcaa cggctttttg
     6601 cagttccaaa gtgtccggga acgcggtcaa cgagtccgct atcatgacag ttgtcctgct
     6661 ggcaggcgtg ggacgcttaa atgccagcca gcctatatca aggccaagtt tttgggctgc
     6721 catcagggcc gccatgccga taaccaggat gccgaggatg atcaggacca catacagggg
     6781 tttggagccg ctccggatca tagaaaggcc tccgcgccct tttcattcca ataggggcaa
     6841 tccttgtatg ctgtgtaaat ctggctatca aagctttcac tcgaaccggg cccgtaacga
     6901 tgcttcacga ccttctcctg atcagttatt ttactctgag agcaagctct taaggggctt
     6961 ttttgctgtc aatctctttt tttgggggct gtgtttgcgc aatcggtggg aacctggcca
     7021 tcgcgggatg cgaagtcagg aagacaaaag cgacccgggg gtgggatacc gggccgcgcc
     7081 tttgtctgcc tgttaccata ttgtctctac gtactcaatc tgtggccctc actctaaaga
     7141 aacggcgggg atgagaacct ggaacagtga agctgttgaa gcctctgccg acgggcgtga
     7201 agagtccgtc atacatatcg gcgtcctcga tgatgtaatc gttggcgcca gtcacttttt
     7261 cccagctgag gatcacgttt gtgccggaag agctgatctg gaggtcctga ggtgcttcct
     7321 gggggtccag atagggccag ctggaataga tgcgaatgtt gtcgatggcg gcagggggct
     7381 gggttccgcc accgctgtcg ttgcgccagc taaagatgag gcgtttaagc tgtcccgcca
     7441 tggcaggatc gagctcaatg ctcacatact gccagtccgg tgaggagtga taggcggtgc
     7501 cgatctggcc atcgctgaaa ctgcttccgg ccacgggagt aaagctggtg tccgtgatgt
     7561 ggacggtgag atagtcgaaa ccagcctccc cggcgcattt atactgaaag cgtagcttca
     7621 taccggccat ggaagctggg aagctgacgt cgtgatagaa gtgggaatag gatcccgaat
     7681 tggggttgta gctgtgagtg gccccataat tggaactgat ataggcgctg tagctgttcc
     7741 ggtagggagc cgcggagccc agttcccatt tgttgggctg gtctgtgttc acagtgatcc
     7801 aatccaccca accgtcctca aagtcctcca cacagggggg ctgcaagggt ccgatccctc
     7861 gtccggtgag cgggatttga tggatggcgc gattctcgat gttgtccacg atcatcagat
     7921 tggcagtgcg gacccccagt tcctgaggcg caaagcttac atagatggtg tagctttctc
     7981 cggccgccac cacgatctcc tccgcgggat cggtgaggac aaagtcatcc tgatgattgc
     8041 cttccagata gacgtcaccc tgcaggatca caaggtccgc gtctccggga ttgctgatca
     8101 cgaaagcctt ggcatcgctg ctctgggaga cgttgatcat gccgtagttc caagaagagg
     8161 gatcgatact gaaggaggga tagggctcgt agaagttgat cgagatgtcg tcaaggctga
     8221 ggtagttgat atagttggtg cagacaccgt ggatccccag ataccagatt ccggtggaag
     8281 gaggagtgaa ggtgccggta cccctttggt aatcgccgtt cacaaactcc tggatgttga
     8341 tgattgtgcc cgtcatgcca gcgatcgagg gagcggggcc ataacggacc tgcaggtaag
     8401 cctgcgctcc ggaagcagtg tattggcgtg cccacagctc caattcatac atctggccgg
     8461 tcgtcaggta gatgggacgg aagatccagg catcaccacc atcgcgcagg gtgaggttgt
     8521 agcttcccgt tttgggctcg cgattgtagt tggtgcttgt attggccgtc cagtagtagc
     8581 ttccactgcc aagcgcctgg ctccatctgt tgatagtagt ggagccgttg gtgttgccat
     8641 cctcaaagct ttcggtgtag ggaaactcca tgatggtgtc atcggcgcgg gtatcaaagc
     8701 tccagatatc cactcccgcg gcagtgccaa ggtcattcac cgcatccacc ttccagtagt
     8761 aggtcctttc aggcaagagg ctgggcgcat agtgggtggc ggtcacattg gtggctatct
     8821 ggggaggagt ttgggtttca ccaaaataga cgatgtagta atccggattg ccacctccgg
     8881 gaccagcggc ccaggtgatg gaagtattca acgcgaggtc gatggcaccg tttgcagggc
     8941 tgagatacac tggtaatgcc ggtggcaatt ggacttccag gaaggcaccc tcatcggctc
     9001 cgatatcagg acggctggca ttacgggttt ggccgtcata atctatgccc acactggcga
     9061 tcacgagcgc gttgccttcc gcgtaggtct ccaccaaagg atcgaggtgc agatcaaagg
     9121 gagacaactt gctgaggaag ggagcgtctt cagagaagga attctggtcc tttccggcat
     9181 tggcggcttt gtatagctcc aaggtctggc aactgctcgt gccatagtag cagatcaggt
     9241 tttgggcgga agggatgccg gcataccaga tgttgttgtt gctggcgtcc gagactgcgt
     9301 caaaatctgg ctgagcgttc cagaaggcca ccgctttgcc tgaggctccg ggcgtgctga
     9361 gattggagaa gatattgttt tgcgggttca ggagagtggg attgttgctg tagagggccg
     9421 cggaagaatg actgccagtg ccgctggaag tgatcagtac gctgttgtaa tacagattgg
     9481 atgtgccggc atacacgtag atgcctctca cctggggcac gctgttcgat gagggagcac
     9541 gcaggtcgca gatcatgttg ttatgggctg ttaccgtgcc gttggtgtat aatccgttca
     9601 caaagctgga gcttccacct gtttggaggt tgtggatatc gttccggtac acggagccgt
     9661 tgctgatgtt cataccatat acgtttccat tcgaactgag cccggaaacg gtgttttgat
     9721 atacaactga ggaattcatg attattccgg caacatcact ggtaccagag gaggcaagat
     9781 cggctaccag gttgtcatac accagtccat tggacccgcc atagtagtta cggatgccgt
     9841 agatgtggct gctggcattg atcccggaga tctgattgga gtagatattg aatttgtcat
     9901 aatggtggta aatgccatta accgtaccag ttgtggatcc accgatgatc tggttgtgat
     9961 ggatttggcc ggtaccactt tcattgtagc agttgatacc cgtgaaattg cctgtgtttg
    10021 tagccgccat cccgatctgg ttgtagctca cctcaaggtt gtttgtatag cgtatgtaga
    10081 taccgtaggt actcacggag gatatctggt taccgctcaa taccagattt tgcgcggcgc
    10141 tggaatcata gagatagata ccgttcttgg gcccggagat gtggttgcgc agatagcggt
    10201 tgcgatgatt gatccggcct gaggcagaat cgctcctgat gccaaaggta tcggtggcgc
    10261 gcgccattgt gatggagcag ttttggatgc tgttgtccgt gcagccatca tcggtctgtg
    10321 cttccagcca atagccatac accagtccgc tttggccggg ctggttggca atgtcgatgt
    10381 gatcgaaggt aacaaaatct gctccctcca gcttgatgcc ataggaacct gccccttcgg
    10441 atggagtgat gagaggattg acagccaggg tatctgtgct ggtaaacacg atggggtctg
    10501 ctgctgtgcc ggaaaccaca agggggggtg gattttcggc aaagacctct gtggcaaggg
    10561 ctttgaaggt gaccccaccg gttcccacac cactggcatt caggtatcta atggcatggg
    10621 tgaaatccgg atagaatccg ctgctgccta ttacccagtt tccggtgagc ggtacctgat
    10681 gggtggagaa ctgccaaaca gggcaatcgt cgggatcagt gctgccgttg tcgttgtagg
    10741 ggacgatctt ccagtagtag gtactcagat aattcagggt aacgggagaa acgtagctga
    10801 gcacgttgcc gatatcgata ccgtccgcga tatccgaggg gggattattg gacccaaagt
    10861 acaccagata acaggtggga gcacctccgg ccccggaagc gtaccatgtg aggctgggat
    10921 tgatgctcac ggcatactca ttcatttggg gcgatacgac taccgccgga gcgggagcat
    10981 tggcaggaac aacgaaattg ccctcatcag ctccgatatc tggagtgctg gcattgcggg
    11041 tctccagatc aatatccgtg acaatgccat ctatgggagt ggcattgctt tcagctatcg
    11101 tggcagcacc aatgggaatg tggatatcat aatccggacc aacgccatcc acaaagggca
    11161 ctgcctctgt gaaactgctc tggtcaaagc tcacagccgc ggctttgtaa tcgtccaaag
    11221 tctcgtaata gaccgatgga gtgcggcaga tgagattgcg gggcccgggc gtgccagcat
    11281 accagatatt gttgtctgta gcagcgttga tattggcaaa tccgtccaca cttttccaca
    11341 gagcggctgt gatgccggca ttgcctggcg tgctggtgtt catgaagatg ttgttgttgg
    11401 c
//
LOCUS       DAOSFN010000079        11384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4807_length_11384_cov_9.655398, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000079 DAOSFN010000000
VERSION     DAOSFN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11384
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4807_length_11384_cov_9.655398"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..792)
                     /gene="rnpB"
                     /locus_tag="PLO35_06245"
     ncRNA           complement(380..792)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLO35_06245"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1154..1960)
                     /locus_tag="PLO35_06250"
     CDS             complement(1154..1960)
                     /locus_tag="PLO35_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOH79305.1"
                     /translation="MYKSAKQSGLEQRILKHLSGKPLEAAQLMFSDPTLQATQEYANI
                     VSIKRLGYNDHGPVHMRKATLNTIKMFELLHGAGIQMNLEKEEAGTAEDSLTAVLFAS
                     LLHDLGMTIARDSHEYLSIQLATPYIDSILDKILGPEEYMRKTAIRSIAIEGIFGHMA
                     NRKIHSLEAGLVLIGDGSDMEKGRARIPSMLSMEPRVGDIHRTSASSIQQVKITKGEE
                     KPICIKVLMSASVGFFQIEEVFFPKINFSPVKPYIELYAGVIDREMLRYL"
     gene            complement(1976..2431)
                     /locus_tag="PLO35_06255"
     CDS             complement(1976..2431)
                     /locus_tag="PLO35_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOH79306.1"
                     /translation="MDTPFSTLTLMQFCWAFLSITGFCIRVNLKSWKILYIALGAGLN
                     WAIYLIVLYFTNSLLLSVFISTILVCSYSEIVARLCFIPVSVFVTCVIIPLVPGSSLF
                     YAMQAYISGDRVLASHHIAQVLMISGTIAMAIAIVASVTNLSQRLINRF"
     gene            complement(2433..3224)
                     /locus_tag="PLO35_06260"
     CDS             complement(2433..3224)
                     /locus_tag="PLO35_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOH79307.1"
                     /translation="MSRIPIREATDIVLEVGRILLTSGATTNRVELMMSKVASSFGYA
                     KTEAFVTPTGIFLTIADGEKDHSTSVKRIEGRRIDLGKITAISHLVSKLDQAFKDPDS
                     PNMSVSKLRAELYKVEHAQPWPTWLQVFCGGGTSGFFCLLFGGSWLEFGVAYLVGILV
                     STTMHLLGMIKINNFLLNALGAMLIVVFAKTLDIWVPYIKLDNIIIGGIMLLVPGLSI
                     VNAIRDTMSGDLVSGTARAVEAIFIAVAIATGSGSMLKLWALMGY"
     gene            complement(3221..>4267)
                     /locus_tag="PLO35_06265"
     CDS             complement(3221..>4267)
                     /locus_tag="PLO35_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79308.1"
                     /translation="ALDSEFIVPRSDRELCSRLSRGADFVLVSESVANLRFRLADDAA
                     TLRLLKRLERLERRRHHAEERVLKRLSRFLSARMPLIRKAFKALEHFSLAFMLADFGW
                     RHDGVVPKLGGKSVRIKGAVNLPLKLHLEAQGRAYQAIDLDFEPRGNLITGPNMGGKS
                     CALQTLGQLVRLTQLRVPLPCREASLPRIDNIWLNQDDSSRGADLSAFGREVVSFTEA
                     LRMPGKSLLLLDEFARGTNPAEGEALLCAILRYLKTTGHLVVAATHFSAPALMKELAQ
                     YTIAGPKLAEQDASSGSPDSPEERLKQLSRQMDYRLKRLARGKVPPHSAIRIATILGL
                     PEAILRFIPEGSKP"
     assembly_gap    4268..4367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4368..4675)
                     /locus_tag="PLO35_06270"
     CDS             complement(<4368..4675)
                     /locus_tag="PLO35_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79309.1"
                     /translation="MIKALVSDALGMPAIYDEIGIPGPWMDELKRLWLKGHSDLRLPG
                     YPEIGRLLARLRENPSLDEAGRWQLGNIPKAFGTESRRDTPYALHELFLIKEFLFHYG
                     "
     assembly_gap    5467..5566
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5567..5843
                     /locus_tag="PLO35_06275"
     CDS             <5567..5843
                     /locus_tag="PLO35_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79310.1"
                     /translation="DTSHPVGGLAPNQLGIYDMSGNVWEWCWDIHDSYPVTPQTNPHG
                     PDSGSYRVARGGSWGSAAMNCTVAHRNYYGVNTYHFHMGFRVCRISP"
     gene            6262..7311
                     /locus_tag="PLO35_06280"
     CDS             6262..7311
                     /locus_tag="PLO35_06280"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79311.1"
                     /translation="MIAFKTGWLDSATATLNIYVLTPPTPSIPAGTYNSPQTVSLSCT
                     TPGATIRYTTNGLNPDTQSTSYSTPLLLTGGTVLKARAYKVNNTGTTKESGVMTAAYM
                     ITTTYPGSFVYVPGGTFNNGTSDVTVSSFNLDRYEVNKSDYNRIMNGTYDYNTVPQGN
                     VSWFNAIAYCNKRSMDEWLTPYYSYSTYGTDPASWPSGWNTSDANHTNISCNWSANGY
                     RLPTEMEWMFAARGGNLTHNYTYSGSNSIGNVAWYSSNSSNATHNIGTLSPNELFTYD
                     QSGNVSEWVWDIYGSYPTGSQTNPTGPTTGTNRIKRGGAYNTAASVCTVSNRVSVLPT
                     SNDGGTGLRLVRERH"
     gene            complement(7485..8717)
                     /locus_tag="PLO35_06285"
     CDS             complement(7485..8717)
                     /locus_tag="PLO35_06285"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79312.1"
                     /translation="MKYHARTLERDILKRMDSPKAVFILGARQVGKTTLLRHLMEALS
                     PEHSLYFDIELPRNLELFSGPIESILAFLRYSRGDSTGRTYVFLDEIQYLEDFSKTVK
                     LFTDHYHEEFKLVMTGSSSLMIKHQFRESLVGRKDIFTLYPLSFSEYCRFTGHDAIAT
                     ELETASFSRHSPLLSIRGEIEELLADYSVFGGYPEIAMLTDREDRARLLSEIVSSYVL
                     KDIRHILKVEKLSELNRLITSLAHNLGKEINLSELAREVGLHRDTLQKYLLGLEESYI
                     IGTVRPFHANYEKEMRKMPKVYFIDPGIRNMLVNDLSALQQRKDRGELAENLFFGHLL
                     RNCGVTTQIRYWKTKSGAEVDFVVSEDGRIEAWEVKYGPGAGKHFQSFTNLYPEARCR
                     VARYKYEYHEGEVPLWWV"
     gene            9095..11308
                     /locus_tag="PLO35_06290"
     CDS             9095..11308
                     /locus_tag="PLO35_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH79313.1"
                     /translation="MKKSLALIICLLLIMGGAHGARKALVIGNAGYGDSPLRNPVNDA
                     NLIATTLRELEFEVQLVTDADLQQMESALNRFITALSPNDEALFYYSGHGANFNGDNY
                     LIPLGWSFTDETELKYKAFNSNLALERLQKARISIMILDACRDNPYRGARSGSKGLAT
                     MQGKSGSQYVIYSTEQGKTAADGSGSNSPFTESLVKHMKSPDRVEDVMKKVAIELKTK
                     TSDKQIPWSGGTLVEDFYFAKALPGTEMRVTPRTQGLEKLTGDLRITSDLELELYVAK
                     KSIGTAKPGYAFNLPDQPVGDLILEFFLENAKYSLKTSIYDNRVTNLEITSETLSQIR
                     NEAFQTTVKSSPDGAEISFKDFPAIKGHTPFVIYDVFPASYELSLKATRYDELRTVIE
                     TGQQRSAEVNLKLAARYAELTIDSEPSSSRIWLNGKDMGTTPASFTGAQQGLDPGSWK
                     LKLEPIDPMYLPVEKDLELKANQLYSEKLVHPQISSGIQISSDIYPIVVSVDEGKAVT
                     LNSTQTIKLTPGSYKVAVRFSGKDSRPIKTWQGTVEISREKQQTLDLKLIPQSGSLSF
                     SSNLGDYDLKVTNKETNQLTKLSRGSLETYPGSFNLEAVKWGHYVINRDIEIKEGLRA
                     EQRLDFEPIPIKMLQPYNTWKTHQKISLASLALTLGISAFTYYRADQAHQDYLAADSP
                     YGAQDARQEFEGQRNIFYGSCGLNILPVAYYIFSLSKAHQSRSAIELEMRSRLRK"
BASE COUNT         2649 a         3109 c         2889 g         2537 t
ORIGIN      
        1 acagggggct atgacattga ctcacccctg cggggttgat tttcgcacaa gctctaaagc
       61 cagtgttacg atcatgtgca acggtcttat caagaactac cgatcctgta gcttatctac
      121 ctataagggt atggtttccg ccgggatcaa tcctatggct gctttcaaag ctggttatcc
      181 ttgccgcaac gctatgttga tgaatatatt gcgcatactt tctgcctgcg cgccgcacta
      241 tcactgtctg gtatctcagg actatctcac cctccaccca tgccaaagca cgggataggg
      301 gtgcgataag ggtgcgataa gcacgggata gtcaagaggg agaatgctgc caaaaagcct
      361 gcaagctctc attccttcca ggactacctg taagccggat tctgtaaccc ccccaggggg
      421 ggccgatgtc catccatcta ggcctgggct cacgctcagg ctcgagctgc ctacccggaa
      481 gcatgatcgc gcgggccgcg ctaagcttcc ctatttggca ttgcaccgga taaggctttc
      541 ctggccgttc gcgtcaccgc gacgccggtg gtctcttaca ccaccatttc acccttaccg
      601 gagcgtcctc agctatgcta aaccgcggtc tcacaacggt atcctgaaag gggcactata
      661 tagctgaaag ggacgtccgg cggtttgttt ctgtggagct ggctccgcgt ttccacggct
      721 tcccgttagg aagtatcccg ccctgtggtg tccggacttt cctccccggg ggtgagcaaa
      781 aaccgctgat tgggacccgt tggagttcaa gcggcctgga taaatgtttg catggcagcg
      841 agtttgcggg ccgcttggac ttcaaccagc acaccggtaa gataagtgcc tccgatgtac
      901 tgcttgaagt ccaaagcccc gtcaggtaaa gaaataaacg tataatctgt ttaggggctt
      961 tgaactccaa gatggcaatt tcgcccaagc tctaaagcca gtgttacggt ctttgctcac
     1021 ccccgcggcg gacatctggt cttcccggaa ggaatgagaa cttgtatgcg cttttgaaag
     1081 aactatttgc ttaatgtatt gtactgggtc ttaatattat actgtcattc ccgcgcaggc
     1141 tgtcatcccg cccttacaga tacctcagca tctcgcggtc tatcactccc gcgtagagct
     1201 cgatataggg tttgacgggg ctgaagttga tcttggggaa aaacacttct tcgatctgga
     1261 aaaagcccac cgaagcgctc atcaagacct tgatacagat gggcttttcc tcgcccttgg
     1321 tgatcttgac ctgctggatg gagctggccg aggtgcggtg gatgtctccc acccggggtt
     1381 ccatggacag catgctgggg atgcgggccc ggcctttttc catgtcgctg ccgtcgccga
     1441 tcagcaccag tccggcttcc aggctgtgga tcttgcggtt ggccatgtgg ccaaagatgc
     1501 cttcgatggc gatggagcgg atggcggtct tgcgcatata ttcttccgga ccgaggattt
     1561 tatccaggat ggagtcgata tacggggtgg ccagctggat gctgagatac tcatgggagt
     1621 cgcgggcaat ggtcataccc agatcatgca agagagaggc aaagagtact gccgtgaggg
     1681 aatcctccgc cgttccggcc tcttcctttt cgaggttcat ctgaatgccg gcgccgtgca
     1741 gcagctcaaa catcttgatg gtgttgaggg tggccttgcg catgtgcacc gggccgtggt
     1801 cgttatagcc caggcgtttg atcgatacga tgttggcata ttcctgggtg gcctgcaggg
     1861 tgggatcgct gaacatcagc tgggccgcct cgagaggctt gccgctgagg tgcttgagga
     1921 tccgctgttc cagaccgctc tgtttggctg atttatacat aagtctttta ttctcctaaa
     1981 atcggttgat gaggcgctgg ctgaggttgg tgactgaggc cacgatcgcg atagccatgg
     2041 cgatcgtgcc tgaaatcatc aggacctgag cgatgtgatg cgaggccagc accctgtccc
     2101 cggagatata ggcctgcatg gcataaaaga ggctgctgcc aggcaccaga ggaatgatca
     2161 cgcaggtcac aaacaccgac accgggatga aacacagcct ggccacgatc tccgagtagc
     2221 tacataccaa tatagtcgat atgaacaccg aaagcaagag cgagtttgta aagtacaaca
     2281 ctatcagata gatagcccag ttcaatccgg cccccagagc gatgtagagg atcttccagc
     2341 tcttgaggtt gacgcggatg cagaaacccg tgatggaaag aaatgcccag caaaactgca
     2401 tcagagtgag ggtgctgaaa ggcgtatcca tctcagtatc ccatcagggc ccaaagcttt
     2461 agcatgctgc cgctgcccgt ggcgatggcg acggcgatga agatggcctc caccgctctg
     2521 gccgtgccgc tcacgaggtc tccgctcatc gtgtcgcgga tggcgttcac gatcgaaagt
     2581 cctggcacca aaagcattat cccgccgatg atgatgttgt ccagcttgat atagggcacc
     2641 cagatatcca gggtcttggc aaagaccact atcagcatgg cgcccaaagc gttgagcaag
     2701 aaattgttga tcttgatcat gcccagtaag tgcatcgtcg tgctcaccag gatgcccacc
     2761 agataggcca ctccaaactc cagccagctg cctccaaaca gcagacagaa aaatccggaa
     2821 gtgcctcctc cgcaaaagac ttgcagccag gtgggccagg gctgcgcgtg ttccaccttg
     2881 tagagttcgg cgcgcagctt gctgacgctc atattggggc tgtccgggtc cttgaaagcc
     2941 tggtccagct tgctcaccag atgactgatg gcagtgatct tacccaggtc gatgcggcgc
     3001 ccttcgatgc gtttgacgct ggtgctgtgg tctttttcgc catccgcgat ggtgagaaag
     3061 atgccggtgg gggtcacgaa ggcttccgtc tttgcatagc caaagctgga ggccaccttg
     3121 ctcatcatca gttccacccg gttggtggta gctccgctgg tgagcaggat gcgtcccacc
     3181 tccaacacga tatcagtggc ttcacggatc gggatgcggc tcatggcttt gacccctccg
     3241 ggatgaatct gaggatggct tccggcaagc ctaaaatagt ggcgatgcgg atagcgctgt
     3301 gaggcgggac cttgcctctg gccagccttt tgaggcgata gtccatctgc cgcgaaagct
     3361 gcttcaaacg ctcttccggg ctgtcaggcg agccggagga agcatcctgt tctgccagtt
     3421 tggggcccgc gatcgtgtat tgggccagct ctttcatcaa ggccggagcg ctgaaatggg
     3481 tggccgccac cacgaggtga ccggtggtct tcagatatct gaggatggcg cagagcaggg
     3541 cttcgccttc ggcgggattg gtccctctgg caaattcatc caggagcagc aagcttttgc
     3601 caggcatccg gagggcttcc gtgaaagaca ccacctctct gccaaaagct gagagatcgg
     3661 caccccggga gctgtcatcc tggttgagcc agatattatc gattctgggc aggctggcct
     3721 cccggcaggg tagtggcacc ctgagctggg tcagcctcac gagctggcct aaagtctgca
     3781 acgcgcagct tttaccgccc atattgggac cggtgatgag gttgccgcgg ggttcaaaat
     3841 ctaggtcgat agcttgatag gctctgccct gagcttccag atgcaacttg aggggaaggt
     3901 tgacggcacc cttgatcctg acactttttc cacccagctt gggcaccacg ccatcgtgac
     3961 gccagccaaa atcggccagc ataaaggcca gggaaaaatg ctccaatgct ttgaaagcct
     4021 tgcggataag cggcatcctg gcgctcagga aacggctgag gcgtttgagc acccgctctt
     4081 ccgcgtggtg acgtctccgc tccagccttt ccagccgttt cagcaacctc agggtggcag
     4141 catcgtcagc cagacggaac ctcagattgg ccacgctctc gctcaccaac acaaaatcag
     4201 cgccgcggct gaggcgtgag cagagctcgc ggtccgaacg gggaacgatg aactcgctat
     4261 ccagggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg ccatagtgaa
     4381 agaggaattc cttgatcaaa aagagctcgt gaagggcgta gggggtatcc ctcctggact
     4441 cggtgccaaa ggctttggga atgtttccca gctgccaacg gcccgcttca tccagactag
     4501 gattctccct cagcctggcc agcagcctgc cgatctctgg atagcctggc agcctgagat
     4561 cgctatggcc cttgagccat aaacgcttga gctcatccat ccaggggccc ggaatcccga
     4621 tctcatcata gatggcgggc atccccaggg cgtccgaaac caacgctttg atcatgtctt
     4681 ttcctcttta gtcatagttt tccatgctgt ctgagggcct ccggctgtca acacttttgt
     4741 ggtttctgga gcaatagaac gcggatgaca cgatgacagg atttgcacga tctaatctgt
     4801 ttagaatgaa tggaacgcag aaaaaaggaa aaaggggatg atggggatcg tatgaattga
     4861 gtatttagga taaagaatta gagatgaacc attacaaaac cccggacaat ctctgggccc
     4921 ccaaactggc tggaccatct ggggatggat cgttggcgtg atcatcatcc tgaatgaagt
     4981 ctattttttg tgagtttctt tcaggcttgt agcgctgcat atcaataacc gggatgatac
     5041 taacatcctc atatagctat aatcgtaatt aattaatggg gtacatgaca tatcactgag
     5101 acgttttacc ccctcgcagc aatcttttat atacttgtta taccattatc ccgaaacacg
     5161 ttaaaataac ttgacagaat ccgcttctga tctatctggt caatatacga gttgtcatcc
     5221 cttgaagtac agcttttgct aaatagatat agaaaagcgg ataccggctg cgcacagatc
     5281 tacacctcag atgcaacgcc ctgagcctgt cacctgcaga cagccagtgg aagcagaata
     5341 agatattgtt tctggaggaa tcatgaaaca aactctgctt atttgcctgt tgctcgtttt
     5401 ttgcgcggga ctgacgtctt tttcaggata tgcagcctca gttgatgaaa tgcttcccga
     5461 tggacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggac acaagccacc
     5581 cggtgggtgg tcttgctccc aatcagcttg gcatttatga catgagcggt aatgtttggg
     5641 aatggtgctg ggatatccat gatagttatc cggtcactcc acagaccaat ccccatggtc
     5701 cagatagtgg ttcctaccgg gtggctcgag gaggtagctg gggcagtgct gccatgaact
     5761 gcactgttgc gcatcgcaat tactacggtg tcaacactta ccacttccac atgggcttcc
     5821 gagtctgcag aatttcccct tgatttttgt gttttgcccc tcttgccttt tgcggatttc
     5881 atggcgagag gacaaagcta tgaagccgtg accatgatga ggtgcagggg tatctatccc
     5941 acctgccaag cccggagggc gatccaatgt tatagcttcc cgtcggggtt gggctgatct
     6001 cctcctgggg tttggatgcc attcctcagt ggtctttcat accgtcatag ccgttatgta
     6061 taaccgtatc actggcttca tagcatgcac gagaatggct tattgatgcc cataaaactg
     6121 cactgagagc tccgtcagga gcggcatttc agttatacga tgcgtacccc accttggtct
     6181 gagctccgtc aggggcgata ttttagatag ctaagcgcac cccaataaat agtttgagtc
     6241 ccatcgggac ggcagtttag attgattgca ttcaaaacgg gatggttaga cagtgctacc
     6301 gccactctca acatctatgt gctcactcct cccactccta gcatcccagc cggtacctac
     6361 aattcccctc agacggtgtc cctatcgtgc accacgccgg gagctacgat cagatatact
     6421 accaatggac taaatccaga cacacaaagc acttcatact ctacacccct tctgctaacc
     6481 ggcggcaccg tattgaaagc cagggcatac aaagtgaaca acacgggtac cacaaaagag
     6541 tcgggtgtca tgacagccgc ttacatgatc accaccacct accccggcag ctttgtctat
     6601 gtccccggcg ggaccttcaa caacggcacc tccgacgtta ccgtgtccag tttcaatctg
     6661 gacagatacg aagtcaacaa atccgattac aacaggatca tgaacgggac ctatgattac
     6721 aatacggtac ctcagggcaa tgtatcctgg ttcaacgcca tcgcttactg caacaaacgc
     6781 agcatggatg aatggctgac tccctattac agctactcca cctatggcac cgatcccgcc
     6841 agctggcctt cgggctggaa taccagcgat gccaatcata ccaacatcag ctgcaactgg
     6901 agcgccaacg gctatcgtct gcccactgag atggaatgga tgttcgcggc ccggggtggc
     6961 aatctgaccc acaactatac ctacagcggg agcaacagca ttggaaatgt ggcctggtac
     7021 tccagcaact cctccaacgc cacccacaat atcgggaccc tgagccccaa tgagctgttc
     7081 acctacgatc aaagcggcaa tgtgtctgaa tgggtgtggg acatctacgg cagctatccc
     7141 accggcagcc agaccaatcc taccggcccc accactggta ccaacaggat taaaagggga
     7201 ggggcataca atactgccgc ttctgtctgc acggtcagca acagggtatc ggtattgccc
     7261 acttccaacg acggcggtac aggattgagg ctggtgagag aacggcactg aaacctgatc
     7321 tgcttttcgg caagctggtc atggtccgcg cgtgagtgag ggccatgacc ggtttttggg
     7381 tgatagggat atagagttgt agagttgtag ggttttgggg ttatagagtt atagcgtttt
     7441 gacgtcagac tcaccagcac gcagtgctct caccagccga aggctcacac ccaccagaga
     7501 ggaacctccc cttcatggta ttcatactta tatcttgcca cccggcatct ggcctcggga
     7561 tagagattgg taaaggactg gaaatgtttg ccagcaccag gaccgtattt cacttcccag
     7621 gcctcgatcc tgccatcttc cgacaccaca aaatccacct ccgcgccact tttggtcttc
     7681 cagtatctga tctgggtggt cacaccgcag tttctgagca ggtgaccgaa gaacaggttt
     7741 tctgccagtt caccccggtc ttttcgctgc tgcagagcgc tcagatcgtt caccagcatg
     7801 tttctgatcc cgggatcgat gaaatagacc ttgggcattt tgcgcatctc tttctcatag
     7861 ttggcgtgga agggccgcac cgtcccgatg atgtatgact cctccagccc cagcagatat
     7921 ttctggagcg tatcgcggtg caagccaacc tctctggcca attcactgag gttgatctcc
     7981 ttgccaaggt tgtgggccag gctggtgatc agacgattga gttcagagag tttttccacc
     8041 ttgaggatat gcctgatatc cttgagaaca taggatgata cgatctcgct tagcagtctg
     8101 gcgcgatctt cacggtcggt gagcatcgct atttccggat aaccgccaaa gacactgtaa
     8161 tcggccagga gctcctcgat ttcgccacgg atgctgagca gagggctgtg gcgcgagaag
     8221 cttgcggttt ccagctcggt ggcgatcgcg tcatgtcccg tgaatctgca atactctgaa
     8281 aaactgagtg gatacagcgt gaagatatct ttgcggccca ccagagactc ccgaaactgg
     8341 tgtttgatca tcagagacga ggagcccgtc atcaccagtt taaactcctc atgataatga
     8401 tctgtaaaga gcttgacggt cttggagaag tcttccagat actgaatctc atccagaaac
     8461 acgtaagtcc ttcccgtaga atctcctctc gaatacctta gaaaggcaag gatgctctca
     8521 attggcccgg aaaaaagctc cagattgcgg ggcagctcga tgtcgaaata tagagagtgc
     8581 tccgggctca gcgcttccat caaatgcctc agcagagtgg tcttccccac ctgtctggcc
     8641 ccaaggataa acacggcctt gggactgtcc atccgcttca ggatatcacg ttccagggtc
     8701 ctggcatggt atttcataaa tcacctcgtc gtcagtttcc ccgttaagcg ttccatgtca
     8761 agactttttt tatagtacat acaacaaaca gacaggtcta tttgccggtt aatatgtaca
     8821 aatagtctgc aaagagggtt gagatgaatg agagcgttga ggaggttttg ggttacaggt
     8881 tacaggtttc ggttgccatc acatgcgtag catctcacca gccgaaggct ctcacatgcg
     8941 cagcatctca cctgcctcag gtatctcacc tacgcagtat ctcacctacg cagcatctca
     9001 cctacgcagc atctcacttt ttccccttga caggaatccc aaaccctcaa acattgccaa
     9061 aatcgcattt gactcaaccc actggagagg atgtatgaag aagagcctgg ctttgattat
     9121 ttgcctgttg ctgattatgg gtggagccca tggggcccgc aaagccctcg tgatcggcaa
     9181 tgccggatat ggcgacagcc ctttgcgcaa ccccgtcaac gacgccaatc tgatcgccac
     9241 caccctgcgc gaactggagt ttgaggtcca gctggtcacc gatgccgatc tgcaacagat
     9301 ggaaagcgcc ctcaaccgtt ttatcaccgc cctctctccc aacgacgaag cgctcttcta
     9361 ctacagcggt cacggggcca acttcaacgg agacaactat ctgatccccc tgggctggtc
     9421 attcacagac gaaacggagc tcaaatacaa ggctttcaac agcaatctgg ccctggagcg
     9481 cctccagaaa gcccgcatta gcatcatgat cctggacgcc tgccgggata atccttaccg
     9541 gggggcccgt tccggttcca aagggctggc caccatgcaa ggcaaatccg gtagccaata
     9601 cgtgatctac agcaccgagc agggcaaaac cgcagccgat ggaagcggtt ccaacagtcc
     9661 cttcaccgaa agcctcgtca agcacatgaa atccccggac cgggtggaag acgtgatgaa
     9721 gaaggtggcc atcgagctca agaccaagac cagcgacaaa caaatcccct ggtctggagg
     9781 cacgttggtg gaggatttct attttgccaa agcgttgccg ggcacggaaa tgcgcgtcac
     9841 acccaggacc cagggcctgg aaaagctcac aggcgatctg cggatcacca gtgatctgga
     9901 actggaactc tatgtggcca aaaagtctat cgggactgcc aaaccaggct atgccttcaa
     9961 ccttccggac cagcctgtgg gcgacctgat cctggaattc ttcctggaaa acgccaaata
    10021 cagcctcaag accagcatct acgacaaccg cgtcaccaac ctggaaatca cttccgagac
    10081 cctcagccag atcagaaatg aagcctttca gaccaccgtc aaatccagtc ccgacggcgc
    10141 ggagatcagc ttcaaagact ttccagctat caaaggccac accccctttg tgatctatga
    10201 cgtcttcccc gccagctatg agctcagcct caaagccacc cgctacgatg agctcaggac
    10261 tgtgatcgag accggtcaac agcgcagcgc ggaagtcaat ctcaagctgg cggcccggta
    10321 tgcggaactg accatcgact ccgaaccatc cagcagccgc atctggctga acggcaagga
    10381 tatgggcacc acccctgcct cttttacagg agcccagcaa ggcctcgatc ccggctcctg
    10441 gaaactgaaa ctggaaccca tagatcccat gtatctacct gtggagaagg accttgaact
    10501 caaggccaac caactctata gtgaaaagct ggtccatcct cagatatcat caggaatcca
    10561 gataagcagc gatatctatc ccatcgtcgt cagcgttgat gagggcaaag ccgtcaccct
    10621 gaactccacc cagaccatca agttgacgcc tggcagctac aaggtggcag tgagattctc
    10681 cggaaaagac agccgtccca tcaaaacctg gcagggaacc gtggaaatat ccagagaaaa
    10741 gcagcaaacg ctggacctga agctaatccc ccaaagcggc agcctgagtt tcagttccaa
    10801 tctcggagat tatgatctta aagtgacaaa caaagagacc aatcaactca ccaagcttag
    10861 caggggatcc ctggagacct accccggcag cttcaactta gaagcggtca aatggggcca
    10921 ctatgtcatc aaccgcgata tagagatcaa agaaggtctg cgcgcggagc agcgcctgga
    10981 cttcgagcca attcccataa aaatgctcca gccatacaac acttggaaaa ctcatcagaa
    11041 gatcagcttg gccagcctcg ctctcacgct tggaatttca gcgtttacct attaccgcgc
    11101 cgatcaagcc caccaggact atctggctgc cgattctcct tatggcgctc aggatgcccg
    11161 gcaggaattt gagggccagc gcaacatctt ttatggctcc tgcggtctga atatcctgcc
    11221 agtcgcctat tacatctttt cactctccaa agctcaccaa agccgctccg ccatcgaact
    11281 cgaaatgcgc tcccgcctga ggaaatgact atgaaaacaa gaaatctact actgctcctg
    11341 ttgcttttgg ctgttgtcag cagttgctgg aagatggata atcc
//
LOCUS       DAOSFN010000080        11092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_4958_length_11092_cov_9.032165, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000080 DAOSFN010000000
VERSION     DAOSFN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11092
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4958_length_11092_cov_9.032165"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLO35_06295"
     CDS             complement(<1..656)
                     /locus_tag="PLO35_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79314.1"
                     /translation="MADKKVEKGALSIHTENIFPIIKKWLYSQHDIFLRELISNAVDA
                     INKRKYADPDFKAEDMKIEVKLDDKKKTLQIIDTGIGMSAEEIRKYINQIAFSGAEEF
                     INKFKDVQSNIIGHFGLGFYSSFMVAEKVSIDSLSYEPGSEPAFWECDGSTEYSMKAG
                     KRKEIGTTITLHFNEDSADYAKESKITEILERYCNFMMWPIMFKDKQVNLKEALWNRN
                     P"
     gene            complement(760..3870)
                     /locus_tag="PLO35_06300"
     CDS             complement(760..3870)
                     /locus_tag="PLO35_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOH79315.1"
                     /translation="MKLAEFSVNRPVMTVMVALIVIILGSVALTRLPIDLMPDITSPT
                     ISVSTSYSKASPLAMEELVTRPIEEALSAVTGVQEISSQSSEGSSNVQVRFNWGTDLE
                     EAANEIRDRLDRIISRLPDGATRPSLRKFDLAAMPVAMMGVTSDLDPLSLRKIIDEQV
                     SYRLERVDGVASINVWGGLTREIKINVDPLKLKALAIPLDQVIARIKAANVNLPTGTL
                     YRGNQQITVRVPGVFNDLDELRHTPVLDRGGNRIYLKDIAEVVDGSSKETRLVRVNGT
                     PGIQISINKRSGTNTVKVAKGVMDEVENINRGVAHVQVVPLVDQSVFIKQAIKNVASS
                     AILGGLLAVLILLFFLRNIKSTAVISTAIPISVIATFGLLYANGFTLNLMTIGALALG
                     VGQLLDNSIVVLENIFRHREMGKSSKDAAIDGAHEVTSPIIASTLTSIVVFLPLLFMR
                     GMTGILFQQLAFVVVFSLLCSLVTALTLVPMLASRMVSVSEEKSLGGKGIKARLFHGV
                     GLILHRMESFYAGALKYALDHRIKISFIALALIGGAILLTGLIDSELMPVSDEGEIRV
                     NLDMEAGTRLEVMDAQVKKVEAKLKGRFPEITHVITSVGGGGWGSSSSNSASLRISLV
                     SKSQREASDEELAGKIRKLVTGLPGVKVRVRTATNNQLSRVMGGGGGRIELQVRGHDL
                     DVSYRLANQIMKAVEGVEGITDVNLSRTSGAPEDLVVIDRSKAAELGLSVQAIAQMLE
                     TVLSGTQAGELQEDGKEYPLIVRIDAAEFMPIMQLLDLSLTNSSGQPIVLRNVVSVES
                     SESSTVIERVNQERMIDVSANLSGRNLSAAIADIQARINQVQMPIGYGFEITGDYKEQ
                     QESFRELFIGIVLALVLVYMVMASQFESVRDPFIVMFSVPFAIIGVALMLFLTKTTFN
                     IQSYIGLIMLGGIVVNNAILLVDTTNRLRRIDGLALREAITTAGRQRLRPILMTALST
                     ILGLVPMAIGAGEGGETQAPLARAVVGGLLSSTLITLILIPVIYSFFEGGFHKLMARI
                     RTKPAG"
     gene            complement(3867..5072)
                     /locus_tag="PLO35_06305"
     CDS             complement(3867..5072)
                     /locus_tag="PLO35_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH79316.1"
                     /translation="MKKKYLWLILVLIVGLLTWQIITRVRQKQSSDKPSGPERGASAI
                     AVETAPAAYRDLSDIGSFSGNLEPKSSFLLAPRVTGQLRKLHVNIGQTVSRGHLVAEL
                     DDRVFRLELEKARSSVAVSRAQAEQAANALRLAELEFDNQRQLFEKGYISRSQFDQAN
                     AQLISDRSKDNVAKASLNSALAAHSAAEIQLSYTRITADWEGGPATRIIGEKLADEGA
                     LLSSGSPVFRIMDISSLIAVIDVIEKDYTRIKLGQSVKVSSDSYPGEEFSGKVLRQAP
                     QLSESSRQARIEIEVPNSGQKLKPGMFARVQVTYAIKKDVLSVPTAALTKFSDKEGVF
                     LVEKGNREARFVALEIGIRGLDYVEVLSPALEGEVVVLGQDLLDDGSKVSLPADAKSS
                     EQTQKRGRG"
     gene            complement(5235..5310)
                     /locus_tag="PLO35_06310"
     tRNA            complement(5235..5310)
                     /locus_tag="PLO35_06310"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5275..5277),aa:Gly,seq:ccc)
     gene            complement(5390..6928)
                     /gene="guaA"
                     /locus_tag="PLO35_06315"
     CDS             complement(5390..6928)
                     /gene="guaA"
                     /locus_tag="PLO35_06315"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOH79317.1"
                     /translation="MKHDSVLILDFGSQYTQLIARALRSLNIYCEIHPYNMSTDAIKA
                     FDPQALILSGSPFSLYQSDSPRPDEIIWELGLPILGVCYGMQLICERFGGQIKRSTKK
                     EYGYAQLEILDDHDLLDGIADGNQVWMSHGDSISELPSVFRLLASTANTPFTAIAHRE
                     LPVFALQFHPEVHHSQNGMRVLENFALKIAGLKADWTSGSFIEGAVAKIRARVGTDRV
                     ILGLSGGVDSSVAAALLYRAIGDRLIPIFVDNGCMRYQEAARVKEFFSCMPDLKIDFV
                     DASQLFLSRLQGVENPEMKRKIIGAAFIDVFEAEAARFPDAKWLAQGTLYSDVIESVS
                     FKGPSVTIKSHHNVGGLPDKMKLGLVEPLREIFKDEVRAVGRQLGLPYELISRHPFPG
                     PGLAVRILGAVDADRVQILQQIDEVFIQELRAWNLYDSTWQAFAVLLPISSVGVMGDE
                     RSYENVCALRAVNSVDGMTADWTELPYPFLKRVSNRIINEVNGVNRVVFDISSKPPAT
                     IEWE"
     gene            complement(6925..7557)
                     /locus_tag="PLO35_06320"
     CDS             complement(6925..7557)
                     /locus_tag="PLO35_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH79318.1"
                     /translation="MLQIEPYILLPEYGTNTWLLYDDEAKEGILVDPSAPSTLLLKRI
                     EALYLKIVMIVNTHGHGDHIGGNGWFRERLNCQLAIHPLDANMLTDNRKNFSDYMGFP
                     LNEKAADLMLADAQTIGLGKHTVRVIHTPGHTAGCVCLLCEKYLISGDTLFEMSIGRT
                     DFPGGSHKAIIDSIRHKLFVLEDDVIVFPGHGPKTSIGMEKKNNPFLRDR"
     gene            complement(7566..8564)
                     /locus_tag="PLO35_06325"
     CDS             complement(7566..8564)
                     /locus_tag="PLO35_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HOH79319.1"
                     /translation="MTKHRREHSAIALFSGGLDSILAVKWMKELGYEVFPLFFAAPYL
                     PPQKALASAEENDIEIDVIDITQAHLQMIQAPLHGFGKHHNPCVDCHALMFRIAGSLL
                     EQYGASFLISGEVLGQRPMSQRRSALHSVANTSGVGDLLVRPLSQKLLPDTKPLREGW
                     VEKEDLLAIHGRGRNAQFDLAQQLGIKSYPPPGGGCLLTDRNFTLRLRDLIEHKLENP
                     YDIELLRWGRHFRLSPTLKLIVGRDEADNANLDLAAGKADKMLIRDVMGPLGVLTGAP
                     PDEDTLALACSILLAYSRKAPDPGYVRYRIDSIDEREILVSKADSDTLSKYKISYD"
     gene            8864..>9687
                     /locus_tag="PLO35_06330"
     CDS             8864..>9687
                     /locus_tag="PLO35_06330"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79320.1"
                     /translation="MLIGVIAVSAVEISPGWTYGAELGITRGNNAGSRENLAPMARGF
                     LQLEIFKFLYMRTGLGYTPLHASKTYATNTFMGDTRFIFSPLHDKKYAPYLFAGAGAS
                     LDMAKGNSDVVPLFPFGLGVELELKKGMDLEVAATYFHSNSDQLDRLTLYDQDTTEKN
                     DGFWGLTAGLTFSDPGPQRKAQPKPVVVEEELEPVPEPVVVTPVVEKPAPVDTRTKDS
                     DQDGLSDYDEANVYMTDPLKADTDGDGLNDGDEIKTHKTDPLRADSDNDGLADGAEL"
     assembly_gap    9688..9787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9788..10420
                     /locus_tag="PLO35_06335"
     CDS             <9788..10420
                     /locus_tag="PLO35_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH79321.1"
                     /translation="DGDGLNDGAEVLNHKSDPLKMDTDADGLNDFDEAVQYKTDPLKG
                     DTDGDTLSDYAEVMQHKTNPLVMDTDKGTVDDGTEVAENKDPLDPKDDVLDLRVGSTF
                     SLEGIFFETAKSNILPESIPKLEQAYAALTANPDVKILIVGHTDSDGSEASNMTLSKN
                     RADAVKKWLVDKGIATDRIRTDGKGESQPKATNDTAEGKALNRRIEFVVE"
BASE COUNT         2533 a         2911 c         2935 g         2613 t
ORIGIN      
        1 ggattgcggt tccagagggc ttccttgagg ttcacctgtt tgtctttgaa catgatgggc
       61 cacatcatga agttgcagta gcgctccaga atctcggtga tcttggattc tttggcgtaa
      121 tcggcggaat cctcgttaaa gtgtagagtg atggtggtgc cgatctcttt gcgtttgccg
      181 gctttcatgc tgtattcggt gctgccgtcg cattcccaga aggcgggttc gctacccggc
      241 tcgtatgaaa gggaatcgat gcttaccttt tccgccacca taaaggagga gtaaaaaccc
      301 agaccaaagt ggccgatgat gttgctctgg acatctttga acttgttgat gaattcctca
      361 gcgccggaaa aggcgatctg attgatgtat ttgcggatct cttcggcact catgccgata
      421 ccggtgtcga tgatctggag ggtctttttc ttgtcatcga gcttgacctc gattttcata
      481 tcctcggctt tgaaatccgg atcggcgtat ttgcgtttgt tgatggcgtc cacggcgttt
      541 gagatcagct cccgcaaaaa gatatcgtgc tgggaataaa gccatttctt gatgatgggg
      601 aagatgttct cagtgtggat gctcagagcg cccttttcaa ctttcttatc tgccatgatc
      661 gtctccttat gtgtatgaac ttgttttctg gagataagat atcagaggcg gagcttttgt
      721 caaggagaaa ttagcagtcg gagtaatgga gtgcttaaat cacccggccg gcttagtcct
      781 gattctggcc atcaacttgt ggaagccgcc ttcaaagaag gaatagatca cggggatcag
      841 gatcagcgtg atcagagtgg aactcagcag cccacctaca accgctctgg caagcggggc
      901 ctgagtttct ccaccttcac cagccccgat ggccataggc accaaaccca ggatcgtgga
      961 tagagcggtc atcagaatgg gacgcagtct ttggcgtccg gctgtcgtta ttgcctctcg
     1021 gagcgctagc ccgtctattc ttctgagccg gttggtggtg tccaccagca agatggcatt
     1081 gttcaccacg attcctccca gcatgatcaa acctatgtag gactgaatgt taaaggtggt
     1141 cttggtgagg aagagcatca acgccacacc gatgatggca aagggcacgc tgaacatcac
     1201 gataaaggga tcgcgcaccg attcaaactg gctggccatc accatataga cgagcaccag
     1261 ggccagcacg atgccgataa agagctcccg gaaggattcc tgctgttctt tgtaatctcc
     1321 cgtgatctca aatccatagc caataggcat ttgaacctga ttgatgcgtg cctggatatc
     1381 ggcaatggcg gcactgagat tgcggccact gaggttggcg gacacgtcga tcatgcgttc
     1441 ttgattgacg cgctcgatca cagtgctgct ttcgctgctt tccacgctca ccacgttgcg
     1501 caacacgatg ggctgcccgg agctgttcgt gaggctgagg tccaaaagct gcatgatggg
     1561 cataaattcg gcggcgtcta ttctgacgat gagagggtat tctttgccat cttcctgaag
     1621 ttcgcctgcc tgggtcccgg acagcaccgt ttccagcatc tgagcgatag cctgcacgga
     1681 aagtcccagc tccgcggcct tgcttctgtc tatcacaacc aggtcttctg gagctccgga
     1741 agtgcgactg agattgacgt ccgtgatgcc ttccacacct tcgacggctt tcatgatctg
     1801 gttggcgagg cgatatgaga cgtccagatc gtgaccgcgc acctgcagtt cgatcctgcc
     1861 tccaccgcct cccatcactc tgctgagctg gttgttggtg gcggtgcgga ccctcacttt
     1921 cacgcctgga agtcctgtta ccagtttcct gattttgccg gccagttctt cgtcactggc
     1981 ttcgcgctga gatttgctca caaggctgat tctgagagac gcggaatttg aagagctgga
     2041 gccccaaccg cctccaccca cggatgtgat gacgtgagtg atttctggaa aacggccttt
     2101 gagcttggct tccactttct ttacctgcgc gtccatgact tccagacggg tgccggcctc
     2161 catatctaga tttactctga tctcgccttc gtcagaaact ggcataagct cggaatcgat
     2221 cagtccggtg agcagtatgg cccctccgat cagggctaaa gctatgaatg atatcttgat
     2281 acggtgatcg agagcgtatt tcaaggcccc ggcataaaag ctttccatcc tgtgcaggat
     2341 caggcccacg ccatgaaaga gcctggcctt gatacctttc ccgcccaggg atttttcttc
     2401 gctgacgctc accatccggc tggccagcat cggtaccagc gtgagggccg taaccagcga
     2461 acagagcaga gaaaatacca ccacaaaagc caactgctga aagaggatac cggtcatgcc
     2521 acgcataaat aacaggggta ggaaaaccac gatggaagtg agggtgctgg caatgatcgg
     2581 cgatgtgact tcatgagcac cgtcgatggc agcgtctttg gagctttttc ccatctcccg
     2641 gtgccgaaag atgttttcca gcaccacgat ggagttgtcc aaaagctgtc ccacacccaa
     2701 tgccagagct ccgatcgtca tcagattgag ggtgaagccg ttggcataga gcaagccaaa
     2761 ggtggcgatc acggaaatag ggatggcagt ggagatcaca gccgtgcttt tgatgtttct
     2821 gaggaagaat agcaggatca gcacggccag caggccaccc aggatggcag aagaggccac
     2881 gtttttgatg gcttgtttga taaagacaga ttggtccacc aggggaacca cttgcacgtg
     2941 ggccacaccg cgattgatgt tttccacttc gtccatcacg cctttggcca ctttgacagt
     3001 attggtgccg gagcgtttgt ttatcgaaat ctggatgcct ggggtcccgt tcacgcgcac
     3061 cagtctggtc tctttggagg aaccgtccac aacctcggct atgtccttga ggtatatcct
     3121 gttaccgcct ctgtcgagga cgggagtatg gcgcagctcg tccagatcgt taaacacgcc
     3181 aggcacgcgc accgtgattt gctgattgcc tctgtaaagc gttccggtgg ggaggtttac
     3241 attggcagct ttgatcctgg cgatcacctg gtccagaggg atggcgaggg ctttgagttt
     3301 gaggggatcg acgttgatct tgatttcgcg ggtaagcccg ccccacacat tgatgctggc
     3361 cacaccatcc actctttcga gccggtagga aacctgttca tcgataatct tgcgcagcga
     3421 tagaggatcc agatcactgg tgacccccat catcgctact ggcatagcgg ccagatcaaa
     3481 cttacgcaaa gagggcctgg tagcaccatc aggcaggcgc gagataatcc tgtccagccg
     3541 gtcgcggatt tcgttggccg cctcttccag atcggtaccc cagttgaagc ggacttgcac
     3601 gttgctgctg ccttccgagg attgggaaga gatttcctga actcccgtga cagcgctgag
     3661 ggcttcctcg atcggcctgg tcaccaattc ttccatcgcc agagggctgg ctttggagta
     3721 agaggttgac acactgatgg ttggtgaagt gatatcgggc atcaggtcta tcggcaaacg
     3781 ggtgagggct acactgccta aaatgatgac tatcagggct accatcaccg tcatcacggg
     3841 acggtttacc gaaaactcgg ccagcttcat ccgcgacccc gtttctgggt ctgttcagag
     3901 ctttttgcgt ctgctggtaa agaaacttta ctgccgtcat ccagaaggtc ctggcccaaa
     3961 accaccacct ctccttccaa agcgggggag agcacttcca cgtagtccag cccgcggatg
     4021 ccaatctcca gagccacgaa tctggcttcc cggttacctt tctctaccag gaagacccct
     4081 tccttatcag agaacttcgt gagcgcggca gtaggaacgc tcaatacgtc ctttttgatc
     4141 gcgtaggtca cctgcactct ggcaaacata cctggtttga gcttctggcc ggaattgggc
     4201 acttcgatct cgatcctcgc ctggcgggag ctttcagata gttgaggtgc ctggcgcaac
     4261 actttgccgc taaactcctc tcccggatag gaatcggagc tcacttttac gctctggcca
     4321 agcttgatgc gggtatagtc tttttcgatc acatcgatga cggcaatcag ggaggagatg
     4381 tccattatcc ggaaaaccgg cgatccggaa ctgagcagag ctccctcgtc ggccagtttt
     4441 tctccgataa tccgggtggc cggtccccct tcccaatcgg cagtgatcct ggtgtatgaa
     4501 agctggattt cggcagccga gtgagctgcc agagcgctgt ttaaggaggc tttggccaca
     4561 ttgtctttag agcggtcgct gatcaactga gcgtttgcct ggtcaaactg cgagcgggag
     4621 atataaccct tttcaaagag ctgccgctgg ttgtcaaact caagctcggc caggcgaaga
     4681 gcattggcag cctgttcagc ctgggcgcgt gacactgcca cactggacct ggctttttcc
     4741 agttccagtc taaagaccct gtcatccagt tcggccacca aatgtccacg gctcaccgtc
     4801 tgtccgatgt tcacgtgcag ttttctaagc tggccagtca ctctgggagc cagcagaaag
     4861 ctgctttttg gctccaggtt tccgctaaag ctgccgatat cgctcaggtc acgatatgcg
     4921 gcgggagcag tctccactgc gattgcggaa gctccccgtt cagggccgga aggcttgtca
     4981 gagctttgtt tttgcctcac ccgcgttatg atctgccatg tcagcaggcc cactatcagg
     5041 accaggatta accatagata tttctttttc atgtttgttt ctctcgcttg tattacatat
     5101 gatagtgcag aattcctgcg tggtaaaata ctatgtgcag caataatgta tacagtggtt
     5161 gatcttaggg acgggatgcg tggttgattt gctgctcaat tatgatgcct gatgacgaga
     5221 gatggccaaa aaactggagc gggcaacggg actcgaaccc gcgaccctca gcttgggaag
     5281 ctaatgctct accaactgag ctatgcccgc tcgctgggtg tcaaattgtg agaagcccgc
     5341 tctttgtcaa gctggatttg ccgggcagtt tcaattagag agatgcccgc tactcccatt
     5401 cgatcgtggc aggcggtttg gaactgatat caaagacgac ccggttgacg ccattgacct
     5461 cattaatgat gcgattggaa acgcgtttca ggaagggata gggcagctcg gtccagtcgg
     5521 ccgtcatgcc gtccacactg ttgactgctc tgagggcgca gacgttttca tagctgcgtt
     5581 cgtcgcccat cactcccaca ctgctgatcg gcaagagcac cgcaaaggcc tgccaggtgc
     5641 tgtcatagag attccaggca cgcagttcct ggatgaaaac ctcgtctatc tgttgcagga
     5701 tttgcactct atccgcatca accgcgccca ggatacgaac cgctaaaccc ggtcctggaa
     5761 agggatgccg gctgatcagc tcataaggca gtccaagctg gcgccccacc gctctgacct
     5821 catccttgaa gatttctctg agtggctcca ccaggcccag tttcatctta tcgggcaggc
     5881 cgcccacgtt gtgatggctc ttgatggtca cggagggacc tttgaaggag acgctttcta
     5941 tgacgtcgga gtataaggtg ccttgtgcca gccatttggc atcgggaaac ctggccgctt
     6001 ccgcttcgaa gacatcgata aaggcggccc cgatgatctt gcgcttcatt tcagggtttt
     6061 ccactccttg caagcgtgaa agaaagagct gggaggcatc cacaaagtct atcttcaaat
     6121 ccggcataca gctaaagaac tctttcactc gcgccgcttc ctgatatcgc atgcagccgt
     6181 tatccacaaa aatggggatc aggcggtctc cgatggcgcg atacaagagg gccgcggcca
     6241 cggaggaatc cactcctccg ctgaggccca ggatcactct gtccgttccc acccgcgctc
     6301 tgattttggc caccgctccc tcaataaaag agccggaagt ccaatccgct ttgaggccgg
     6361 caatcttgag cgcgaaattc tccaagaccc gcatcccgtt ttgggaatgg tggacctccg
     6421 gatgaaactg cagggcaaag acaggcagtt cgcgatgcgc gatcgccgta aagggagtat
     6481 tggcagtcga ggccagcagc ctgaacacgg agggtagctc tgaaatcgaa tctccgtggc
     6541 tcatccacac ctggttgcca tccgcgatgc cgtccagcaa gtcatgatcg tccaggattt
     6601 ccagctgggc gtagccgtat tcctttttgg tgctgcgctt gatctggccc ccaaagcgtt
     6661 cgcagatcag ctgcatgcca taacagacgc ccaggatggg caaacccaat tcccagatga
     6721 tctcatctgg cctgggagaa tctgactggt agaggctgaa ggggctaccg gagaggatca
     6781 gcgcctgggg atcaaaagct ttgatggcat cggtactcat attataagga tgaatttcac
     6841 aatagatgtt gagagagcgc aaagctctgg cgatcaactg agtgtattga gaaccgaaat
     6901 ccaggatcag gacgctatcg tgcttcatcg gtccctcaaa aagggattgt ttttcttctc
     6961 catcccgatg gaagtcttgg gtccgtgccc tggaaacacg atcacgtcat cctcgaggac
     7021 gaagagcttg tggcggatcg aatcgataat ggctttgtga ctgccacccg gaaaatcggt
     7081 gcggccgata ctcatttcaa agagggtgtc gccgctgatc agatatttct cacacagcag
     7141 acatacgcag ccggctgtgt ggccgggtgt gtgaatcact ctcaccgtgt gctttcccaa
     7201 cccgatagtc tgagcgtcag ccagcatcag gtcggccgct ttctcgttca gcggaaagcc
     7261 catatagtcg ctgaagttct tgcggttgtc ggtcaacatg ttggcatcca gtggatggat
     7321 ggccagctgg cagttcagcc gctctctaaa ccagccgtta ccgccaatgt gatccccgtg
     7381 accatgcgtg ttcacgatca tcacgatttt caggtacaaa gcctcaatcc gcttcagaag
     7441 cagagttgac ggagcggagg gatcgacgag gattccctct ttggcttcat cgtcatacag
     7501 gagccaggta ttggtgccat attctggcag gaggatatat ggttcgatct ggagcatctt
     7561 aaatcctaat cataagagat tttatattta cttagcgtgt cggaatcagc tttggaaacc
     7621 agaatttccc gctcgtctat gctgtcaatc cgatatctca catacccggg atcgggagct
     7681 ttgcggctgt aggccagcaa gatagagcaa gccagcgcca gtgtatcttc atcaggagga
     7741 gcgcctgtca gcacccccag aggccccatc acgtctctga tcagcatctt gtctgccttc
     7801 ccagccgcca ggtcgagatt agcgttatcc gcttcatcgc gtcccacgat cagtttcaga
     7861 gtgggtgaga ggcgaaagtg cctgccccat ctcaacaatt caatatcgta ggggttttcg
     7921 agcttgtgct caatcaggtc tctgagcctc aaggtaaaat tgcggtccgt gagcaagcaa
     7981 ccacctccgg ggggagggta acttttgatg ccaagctgtt gggccagatc aaactgagca
     8041 ttgcgtcccc ggccgtggat agccagcaag tcttcttttt ctacccagcc ttcccgcaga
     8101 ggttttgtgt caggcagcag tttttgagaa agcggccgaa ccagcaaatc tcccacaccg
     8161 ctggtgttgg cgaccgagtg taaagcactg cgccgctgcg acatcggcct ctggcccaga
     8221 acttcacccg agatcagaaa gctggcgcca tactgctcaa gcagggaccc cgcgatcctg
     8281 aacatcagag cgtgacaatc cacacaggga ttatggtgtt tcccaaatcc gtgcaggggt
     8341 gcctgaatca tttgcagatg tgcttgagtg atatcaatta catcaatttc gatgtcattc
     8401 tcttcagcac tggccagcgc tttctgagga ggcagatagg gggccgcaaa gaagagcgga
     8461 aatacctcgt atcccagctc tttcatccat tttacggcca ggatgctgtc caaccctcca
     8521 gaaaacaggg ctattgctga gtgctcgcgt ctatgcttgg tcaattctga ttccttggtt
     8581 aacttatttg ttccacgctt atttattcgg tccatgagtc aagcccaaat cgccagcacg
     8641 gatgttctta tccgccaagt gagcccagat catcatctta agtgatgcca ggaaatgggt
     8701 aaaggtagtg caaggttttt ttttctagca gataaaagct tgagtatgta cttccatcaa
     8761 gttttgcttg acagaatgat atatcaaatt agatagtata tatataatta tcgtagcaaa
     8821 ggagcttgag ataaagaaaa aaactattct actggttttg atgatgctaa tcggcgttat
     8881 cgcggttagc gctgtagaga tcagtcctgg ttggacttat ggcgcggaat tgggtatcac
     8941 ccggggcaac aatgccggca gcagagaaaa tcttgcgccc atggcccgag gtttcctgca
     9001 attggagata tttaagtttt tatacatgcg caccgggttg ggctacactc ccctgcatgc
     9061 ttccaaaacc tatgccacca acacctttat gggagatacc cgttttatct tcagtccgct
     9121 tcatgacaag aagtatgccc cctatctgtt tgctggagca ggggcctctc tggacatggc
     9181 gaaaggcaat tctgacgtag tgccgctctt cccgtttggg ttgggagtgg aactcgaatt
     9241 gaaaaaaggg atggacctgg aagtagctgc cacatatttt cacagcaatt ccgaccagtt
     9301 ggacagactg acgttgtatg atcaggacac cactgaaaag aacgacggtt tttggggcct
     9361 tacggctggg ttgacattct cagaccccgg tccgcagcgc aaagctcagc ccaaacctgt
     9421 ggtggtggaa gaggaattgg agccggtccc tgaacctgtg gtagtaactc ctgtggtgga
     9481 aaaacccgct ccggtggaca cgcgtacaaa ggactccgac caggacggcc tttccgatta
     9541 cgatgaggcc aacgtctata tgacagatcc cctcaaggct gacacggatg gcgacggctt
     9601 gaacgacgga gacgaaatca aaacccacaa gaccgatccc ctgagagccg acagtgataa
     9661 tgacggtctc gccgatgggg ctgaactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnngat ggcgatggcc tcaacgacgg cgcggaagtg ctaaaccaca agtccgatcc
     9841 cctcaagatg gacaccgatg ctgacggcct caacgacttt gacgaagcag ttcaatacaa
     9901 gactgatccc ctcaaagggg acactgatgg cgacactctc agcgattatg ccgaagtgat
     9961 gcagcacaag accaacccgc tggtgatgga taccgacaaa ggcaccgtgg atgacggcac
    10021 agaagtagcc gaaaacaagg atccgcttga tcccaaggac gatgtgctgg atctcagagt
    10081 gggatccacc ttctctctgg aagggatttt ctttgaaacc gccaaatcca acatccttcc
    10141 agaatctatc cccaaactgg aacaagccta cgccgccctc acagccaacc cggacgtcaa
    10201 aatcctgatc gtgggccata ctgacagcga tggcagtgaa gccagcaaca tgaccctctc
    10261 caaaaaccgt gccgacgcgg ttaagaagtg gctggtggac aaaggcatcg ccaccgaccg
    10321 cattcgcacc gatggcaaag gagaatccca gcctaaggcc accaacgaca ccgcggaagg
    10381 taaagccctc aaccgccgca tcgagtttgt ggtagagtaa ttcaatcaac ctttcaccta
    10441 taggggagag cttgctctcc ccattttttt gcccgcggaa aggcttctcg ggtttcctgc
    10501 cccttcatcc gcagtggaga tcccgtggcc tctccggcga aaatcgcggg agaacgcacg
    10561 gcatctcgag ggcggccaac aggcaaggat gtggaggaaa gcagcgtagc aggagggaac
    10621 tactctgaac tcaaccggga gtatgatatg aagattagcg gttctatggg cgttagataa
    10681 aaaacttatt ccaccgcgtt ggtgttgatt gccatggcgt ttacttgcct ctttcctgat
    10741 agaatgcctc attctcaatc acagcaagcc ggacccaatc cggataaaag aggcttgggt
    10801 cgggccagct ctgccgatgg ctgtgggcag tttataggaa agggaggata aatgagcaga
    10861 tgcaaaatgc cgtaacgcct tgataatagt tacctaacag aagcttttca accttcgtag
    10921 cattggcttc agccggtcgt agcattggct tcagagcttg tgcgaaaatg gcgcattgag
    10981 tcccatgggg acgcaggctg ttgacaaagt atcaaatcag atagaacgca gatgacggga
    11041 tgacacgatc atcaagattc cggacattgt aataggctgt atgtcagatc at
//
LOCUS       DAOSFN010000081        10908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5059_length_10908_cov_7.984797, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000081 DAOSFN010000000
VERSION     DAOSFN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5059_length_10908_cov_7.984797"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..3111)
                     /locus_tag="PLO35_06340"
     CDS             complement(202..3111)
                     /locus_tag="PLO35_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOH79322.1"
                     /translation="MPSLERKIVITDIGSTTTKAILLENAAGVPKLLAINSHPTTVES
                     PHNDVRYGVKHAILGLEKITGAKLLDDKTASPELLFREDISYLSTSSAGGGLQILVIG
                     LTLFDSASSAKRAAYGAGGIILDTFAIDDKRQASQQMLAMRNLHPDMILLCGGTDGGA
                     ISGVLRLAEIMRVAKPQPKYETSAKIPTLYAGNQEAIPMIKHMVSNDFDLYILPNLRP
                     SMEMENLQPTQARIQELFMENVMERAPGYSSLKRKMSSPILPTPVGVLKSLAIIAAQD
                     ERNVFAFDIGGATTDVFSQINGHFQRTVSANLGMSYSALNVMKECGIENVMRWLPSQL
                     DEDLVRDYVANKCLKPTRLPAGDDEVLIEQALAREALRIALAQHRDMHFNGQKIGYLD
                     SIVANDVDGFDRKFNYQHYEQQYDFHESDIDLIVAAGGIFAHNPDPLVSAMIVIDAIR
                     PKGITEIAVDRDFTSPHLGVLSASDPAMAQSLLLGNCLLKLAWHVAPVYPRGHKKGSL
                     TVLLSKDGHEERLEVENESLQVLPAGQKTMRFETSGKAYLSLKNVPQELSTDLPVIID
                     TRTELSSPAKTSSARPQLSKPQEQPLDNMHAAAMSSPSERTREILLPYKGEIKVSPGD
                     IVQPDQVVAVNRFNPPRLFIVDRHSNYEGLSQEQIQSSFLVKVGDELDFDQIFAEVPD
                     NPEWPRYLRNSRRIASPVSGRVEYIDSSTGLMVLSETQNYSVKPISVNLAEQLGVPAK
                     RVARYLDKKVGDFVFRGDPLASRKDQSPEGPIFRFVKAPATGTIISIDKESAVLILKY
                     QAQPMEFRSHVRGKVVDITDGQSLTLSYMASRLDGILGVGKDCSGLLSWLDDPAAKLS
                     METKGRIVACAFAPSAEDLRFFSTAGIAGLICFSMREKVLRDFMGNDLGVINTGNEAL
                     PFSILILNDFSSQPMPSTMQSSLKAIAGAHCYLAPHTRIRAGVVRPFADFIQGGQ"
     gene            3305..3721
                     /locus_tag="PLO35_06345"
     CDS             3305..3721
                     /locus_tag="PLO35_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOH79323.1"
                     /translation="MPAKIITGKVIKVHDGDTITVLEGRAQHKIRLDGIDCPELGQAY
                     GRRAREFTSTLVYGNQVKVIYSERDRYDRVLGMVYTMGGANLNHELVRAGLAWHYKYY
                     SQDKKLAALEKAARKANRGLWKDKSPVPPWQFRQRK"
     gene            3743..4132
                     /locus_tag="PLO35_06350"
     CDS             3743..4132
                     /locus_tag="PLO35_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79324.1"
                     /translation="MARLELFVFSLLLSVILETGLILVLLRFWPSLNKEAVVNYPKVS
                     LICVGATLISISWLWWIFPLWMGASVLYLPVGELLVTLFEGMMYKVLLSLKWSGALLL
                     SALANGFSFTVGIMLFVLVPGFANLSL"
     gene            4142..5227
                     /locus_tag="PLO35_06355"
     CDS             4142..5227
                     /locus_tag="PLO35_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79325.1"
                     /translation="MRAKLIIWVVILLFVSTLMADAGLPIPGPRKPRQSHRDVYFTII
                     RGLDQFPGLVFYNLSYSGIDASFSLVQAESDSLVFSRKADDAHRRYLLWRTQAERFDL
                     DSLSQYSDELNAMMTKGTAELIPVELPVCQGFAEVRQYARLIKAAGDIPKLEMGERVY
                     SLRDFTITAKADFGKIPAFIAKRLERYEWKPRLLMESDEKALLQFLKDDPNHTITREG
                     GPSNSKPGGLNSLKERWQELSLRYPGALMIIFWLLLLILPPVLKTLVMKLIAHPLLHK
                     DRQPANRFWIMVPIAALVQFVLLLVMAPLVLTGWGIILIIPVALVADYLAFRKRLDLS
                     PWQLTLASLLSGLAVLTLWGIMIYFSY"
     gene            complement(5195..6238)
                     /locus_tag="PLO35_06360"
     CDS             complement(5195..6238)
                     /locus_tag="PLO35_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79326.1"
                     /translation="MELLMLIVLSLGHLVIDLGSGTVLYSLGYLFMAGTGDLIRHAVA
                     YNFLAFGMQPVWGLAIDYTKSARWAAVLGGAMIFSALLMLEWPVTANVIASTGNALFH
                     LGGGIMAWRLYPGKAWPLGVFVGPGALGIALSRTTAANTGFWIPVILAMLLFVTLLII
                     VIPGIHTKLKSPYLPRVSRQAVVIVLLLSLSTVIRSWEGLSIGFSWKSQAGMATLLMF
                     TVALGKMLGGFAADRLHRVAAPVVALIIAAPLLAWGAGYPASGLIGTLLFQSTMAITL
                     LGLFEQIPKFPGLAFGILCFCLWTGILLKDWIKLPSQGFWHFALILLSCLALLAALNP
                     RKRSVREVDHYTP"
     gene            6453..7739
                     /locus_tag="PLO35_06365"
     CDS             6453..7739
                     /locus_tag="PLO35_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOH79327.1"
                     /translation="MAAKTIPLFSDADLKGKIVLVRMDHNVVKKGKIKDTMRIDASLP
                     TLREIIKAGGLPVVMTHVGRPYDKKTGLITISDDESVAPIVTYLDAQLGLKGLIPELP
                     ASEEHGIIDLDPLAAAITKLKNAEVDYLYLPNTRWFKGEEAKDASADVFAKALASYAD
                     LYVNDAFGSWQAHASTYNITRYLPSYAGLLMLKEIDSLRQVFDPQRPLVAVVAGAKFD
                     TKIGPLSSLIKICDQLVLGGVIYNAYLAHKYGLKIKGVADEDVAMAAKFVSEAGAYAS
                     RIVELPVILESDSIEARQEGAYRALPLKDLKPGTELNYILDAAPQSFEDPAIKGVFIN
                     ASTIFVNAVMGYTALFGEGTAAMYSLIDQNPGAQKLFGGGDTIQDYKALLPELFSKAA
                     RDPHYYFFTGGGAILDAIEQGSPYGMKPVAALISNK"
     gene            7764..9839
                     /locus_tag="PLO35_06370"
     CDS             7764..9839
                     /locus_tag="PLO35_06370"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOH79328.1"
                     /translation="MESETKNGLNLLAEFPPHTLEEWKQAVIGSLKGADYDKTMLTKT
                     YEGITLKPIYTKADMEGKPWLASLPGQAPYLRGDNPDGYTEGSWIVAQAQDEKSLVKL
                     NKIILDELYRGLNCVHLKPHPATLAARLPHKDDLDKRGVTLNNLDDLKTALRDVDLKA
                     VPFVMFGNKSMILVMGMLNAWAKSQGVALRDLHGSIACDPFRDEEPDFDILAQMTRWV
                     DLKAPRLGTILLDGAAYEAQGASAVQELAYSLASAHQIIGELTQRGLSIEQLAPRFNL
                     SLSLGSNLFMEIAKIRAARMLWSQLIKSWGGSENAQRIWIHGVTAAFNKSSYDVWVNL
                     LRTTTEGFAGVIGGVDSLEITPFDDTVRVADEFSRRIARNQQLILAEEAHFTKVVDPA
                     GGCWYVEALAAELCEKAWGRLQEIEAAGGFLSSLQAGTLDMELQTLAKARIAAVNTRR
                     DVKVGVNMFANPGDELLKAPSADPAWLDEAMERYAELKKRPSSGLEDSLKLINEDRNN
                     DFMVDMICDAWLHNADIEQIASALGWNIKLRRMDKERSVGMMEALRMAVEASSMDTRV
                     QLLNMGSVAQYKARADFSSGFFQTAGFKLLDQSGFDTPDLAIAAVAETQVPAVCICST
                     DDLYVDLVPRLCTGLKRLPKPPVIILAGYPADKVEDYKAAGVDIFIHIRANNYDTNKE
                     LAMRLGVTL"
     gene            9857..>10908
                     /locus_tag="PLO35_06375"
     CDS             9857..>10908
                     /locus_tag="PLO35_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOH79329.1"
                     /translation="MNPDFKPGTAAPKTSAPVWKTNEQIEVKALFTAEDMQGMQHLDY
                     LSGLPPFLRGPYPSMFVQRPWTIRQYAGFSTAEETNAFYRRNLAMGQKGLSVAFDLAT
                     HRGYDSDHPRVVGDVGKAGVAVDSILDMKILFDHIPLDQMSVSMTMNGAVIPIMAFYI
                     VAAEEQGVKPEQLTGTIQNDILKEYMVRNTYIYPPEASMRIIADIFSYTSKHMPKFNS
                     ISISGYHMHEAGATADLEMAYTLADGLEYARAGIKAGIDIDVFAPRLSFFWAEGMNYF
                     MEIAKLRGARVLWAKIINSFNPKNPKSLALRTHSQTSGWSLTEQDPYNNVARTCIEAL
                     AATLGHTQSLHTNSLD"
BASE COUNT         2492 a         3038 c         2983 g         2395 t
ORIGIN      
        1 cggattatgt atttcccgca gattgcgcag attggcccgc agatcacgca gatagtctaa
       61 aggtagagaa gttgagaagg ttgagtaagt tgaaaggtat cagcgcttga atccgcagcg
      121 acctatggac cagccgcgaa atccgcgtaa aatctgtgca atccgcgggt actatacctg
      181 gtttcgtgaa cggatatccg gctactgacc gccctggatg aaatcggcaa agggccgcac
      241 gactcccgct ctgattctgg tgtggggagc aaggtagcaa tgagcgcccg cgatggcttt
      301 caggctgctt tgcatggtgg aaggcatcgg ttgggaggaa aagtcgttca ggatcaaaat
      361 gctgaagggc agcgcttcgt ttccggtgtt gatgacgccc aggtcgttgc ccataaagtc
      421 ccgcaacact ttctcccgca tactgaaaca gatcaaaccg gcaatcccgg cagtgctgaa
      481 aaatctcagg tcctccgcac tgggggcgaa ggcgcaggcc acaatcctgc ctttggtctc
      541 catactgagt ttcgcggcgg ggtcatccag ccagctcagg aggccggaac agtctttgcc
      601 aacccccaga ataccatcca gccgggaagc catatagctc aatgtaagtg actggccatc
      661 tgtgatatcc accactttgc cgcgcacatg ggaccggaac tccatcggct gggcctgata
      721 tttgaggatg agcacggcgc tttctttgtc tatggagatg atggttccag tggcgggtgc
      781 tttcacgaag cggaagatgg gcccctcggg gctttggtcc ttgcggctgg ccaagggatc
      841 gccacgaaac acgaaatcgc ccactttctt gtccagatag cgcgccaccc gttttgctgg
      901 gacacccagc tgttcagcga ggttgacgct aatgggcttc acgctgtagt tttgggtctc
      961 ggagagcacc atcagacctg tgcttgagtc gatatactcc actcgaccgc tcaccggact
     1021 ggcaatgcgg cgggaattgc gcaagtatct gggccattca ggattatcgg ggacttccgc
     1081 gaaaatctgg tcaaaatcca gctcgtcgcc caccttgacc aaaaacgagc tttggatctg
     1141 ctcctggctc agtccctcat agtttgagtg gcggtccaca atgaacaatc tgggtgggtt
     1201 aaagcggttt acagccacca cctggtccgg ctggacgatg tctcccgggc tgactttgat
     1261 ctctccttta tagggcagga ggatctcacg cgtcctttca ctgggactgg acatagcggc
     1321 cgcgtgcata ttatcgagag gctgctcctg gggtttggag agctgaggcc gcgccgaact
     1381 ggtctttgcc ggggaactca gttccgttcg ggtgtcgata atgacaggca gatcggtgct
     1441 gagctcctgc gggacgtttt tcaggctgag gtaggctttt cccgaggtct caaatctcat
     1501 agtcttttgg cccgcgggga gcacctggag agattcgttc tccacctcta aacgctcctc
     1561 gtggccgtcc ttgctcagca ggaccgtcag gctgcccttt ttatgtcccc tcggatacac
     1621 cggcgccaca tgccaggcca gtttgagcag gcaattgccg agcagcagag actgcgccat
     1681 ggcaggatcg gaagcgctca aaactcccag gtgggggctg gtgaaatcac ggtccacggc
     1741 gatttcggtg atacctttgg ggcgaatagc gtcgatcacg atcatagcgc tgacgagggg
     1801 atcgggatta tgggcaaaga ttccccccgc ggccacgatc aggtctatgt cgctttcgtg
     1861 aaaatcatac tgctgttcat aatgctgata gttgaacttg cggtcaaatc cgtccacatc
     1921 attggcgacg atgctgtcca gatagccgat tttctggccg ttgaagtgca tgtcacggtg
     1981 ttgagcgagg gcgattcgca gtgcttctct ggccagggcc tgttctatga ggacttcatc
     2041 atcgccagcg ggcagacggg tgggttttag gcatttgtta gccacataat cgcgcaccag
     2101 atcctcatcc aactggctgg gaagccatct catgacattc tctatcccgc actctttcat
     2161 cacgttgagc gcgctgtagc tcatgcccag attggcgctt acggtgcgtt gaaaatgtcc
     2221 gttgatctga gaaaacacat cggtcgtagc gcctccgata tcaaaggcaa acacgttgcg
     2281 ctcatcttga gcggcgatta tggccagcga cttcaggacc cccaccggag tggggaggat
     2341 aggactgctc attttccgtt tgaggctgga gtatcctggc gctctttcca tcacgttttc
     2401 catgaagagc tcctgaatcc tggcctgagt gggctgcagg ttttccatct ccatgctggg
     2461 gcgcaaattg ggcaggatgt agaggtcaaa atcattgctg accatatgtt tgatcatggg
     2521 aatggcctcc tgatttccag catagagcgt ggggattttg gcgctggtct catacttggg
     2581 ctgaggcttt gccacccgca tgatttctgc cagccgaagg actccgctga tcgctccacc
     2641 atccgtgccg ccacacagca agatcatatc aggatgcagg ttgcgcatcg ccagcatctg
     2701 ttgactggcc tgacgcttat cgtcgatggc aaaggtatcc aaaatgatac ctcccgcccc
     2761 ataagcggcg cgtttggcac tgctggcgga atcaaacaac gtgagcccga tcaccaggat
     2821 ctgaagtccc cctccggcac tgctggtgct gaggtagctg atgtcctcgc ggaagagcag
     2881 ttcagggctg gcggttttgt cgtccaggag cttggcgccg gttatcttct caaggcccag
     2941 gatggcgtgc ttgaccccgt agcgaacatc gttgtgaggg ctctccacgg tggtgggatg
     3001 tgaattgatg gccagaagct tcgggacccc tgccgcattc tccagaagaa tggctttcgt
     3061 ggtggtgctt ccaatgtcgg tgatgacgat tttgcgctct agacttggca tttggcctcc
     3121 tatttgcagt caatacctca tatagcggat tgaaaatatg tgcagttatc ctgtcaagct
     3181 aaatcaccgc ttcagaaata attcttgttc ccaagcggca tatccttagt tttttatcag
     3241 gtaagctatg aaaaagtatc caataatctt aattgccata ctgctgttga ccagctgtgg
     3301 tcgagtgcct gccaaaatca tcactggcaa ggtgattaag gtgcacgacg gagacacgat
     3361 caccgtcctc gaaggtcgcg ctcaacacaa gatccgcctg gatgggatcg attgccccga
     3421 actgggacag gcttacgggc gacgggcccg ggagtttaca tccaccctgg tctatggcaa
     3481 tcaagtgaag gtaatctatt cagaacgtga cagatatgac cgcgtactgg gtatggttta
     3541 cacgatggga ggggccaatc tcaaccacga acttgtgcgg gccggcctgg cctggcatta
     3601 caaatattac agccaggata aaaagctggc ggccctcgaa aaggcagccc gcaaggccaa
     3661 ccgggggctc tggaaggata aaagccccgt gcctccctgg cagttccgcc agcgaaaata
     3721 gcctccggat ggaagacctt gtatggccag gctggaattg ttcgtgtttt ctctgctgtt
     3781 atcggtgatc ctggagacgg ggctgattct ggtgctgctg agattctggc cctctctaaa
     3841 caaagaagct gtagtcaatt atccgaaagt ctcgttgatt tgtgtgggtg ccactctgat
     3901 cagtatctcc tggttgtggt ggattttccc gctctggatg ggggcttcgg tattatatct
     3961 gcccgtgggg gaattgctcg tgaccctgtt tgagggcatg atgtacaaag tgctgctttc
     4021 cctcaagtgg agcggcgccc tgttgctatc cgctctggcc aatgggtttt cctttacggt
     4081 ggggatcatg ctttttgtct tggttcccgg ctttgcgaat ctttctttat aaggagtaaa
     4141 tatgcgcgcg aaactgatta tttgggtggt gatactgctg tttgtctcaa ccctgatggc
     4201 cgacgccggc cttcccatcc cgggaccccg caagcccagg cagtcgcacc gggatgtcta
     4261 tttcaccatt atcagaggac tggaccagtt tcccgggctg gtgttttaca acctcagcta
     4321 tagcggtatc gatgccagtt tcagtctggt ccaggcggaa agtgacagcc tggttttcag
     4381 ccgcaaagcc gatgacgccc accggcgcta tttactgtgg aggacccagg cggagcgttt
     4441 cgatctggac agtctcagcc aatacagcga tgaattgaat gctatgatga caaagggaac
     4501 ggctgagctc ataccggtag aacttccagt ttgccagggt tttgcggagg tgaggcagta
     4561 tgccaggctg atcaaagcgg cgggggatat cccgaaactg gaaatggggg agagggttta
     4621 cagcctcaga gacttcacca tcacggccaa agcggatttt ggcaagatcc cggcttttat
     4681 tgcaaagaga ctggagcgct atgaatggaa accgcgcttg ctgatggaaa gcgacgagaa
     4741 agcgctgctg caatttctga aggacgatcc caatcatacg atcacccggg agggtgggcc
     4801 ttcaaacagc aagccgggcg gtttgaatag tctcaaagag cgctggcagg agcttagtct
     4861 gagatatccc ggcgccctga tgatcatctt ctggctgctg ctcctcattc tccctcccgt
     4921 tcttaagacg ctggtgatga agctgatagc ccatccgcta ttgcataagg acaggcagcc
     4981 tgctaatcgt ttctggatca tggtcccgat agccgcgttg gtccagttcg ttttactact
     5041 ggtgatggcg cctctggtcc tgaccgggtg ggggattatt ctgatcatcc cggttgcgct
     5101 ggtagcggat taccttgcct tcaggaagcg cctggacctc agcccctggc agttgaccct
     5161 ggcgtctctg ctctctggtc tggcggtgtt aaccctatgg ggtataatga tctacttctc
     5221 ttactgagcg cttgcgggga ttcaaggccg ccaacagggc caggcatgat aatagaatca
     5281 gagcaaaatg ccaaaaaccc tgcgaaggca gcttgatcca gtctttcagc agtataccag
     5341 tccacagaca aaaacagaga atgccaaacg ccaggccggg aaacttggga atctgctcaa
     5401 agaggcccag gagggtgatc gccatcgtgg attgaaacag cagcgtgccg atcaatcccg
     5461 aggctggata tcccgctccc caggccagca atggagcggc tataatcagc gctacgactg
     5521 gcgcggcgac cctgtgcagc cggtccgcgg cgaagccgcc aagcatcttt cccagtgcca
     5581 ctgtaaacat cagcagggtg gccatgccgg cctgagactt ccagctaaaa ccgatgctga
     5641 gtccctccca ggaacggatc acggtggaga gcgacagcag cagcacgatc acgaccgcct
     5701 gcctggaaac ccttggcaga taagggctct tgagctttgt gtggatgcct gggatcacga
     5761 tgatcaaaag agtcacgaac agcagcattg ccaggatcac cggtatccag aagccggtgt
     5821 tggctgcggt agttctggaa agggcgatcc cgagcgcgcc agggcctaca aacacaccca
     5881 gaggccatgc tttgcccgga taaagtctcc acgccataat cccgccgccc agatgaaaga
     5941 gagcgtttcc ggtggaggcg atcacattgg ccgtcacagg ccactccagc atcagcagcg
     6001 cggagaaaat catcgcgcct cccagcacgg ctgcccagcg ggcgcttttg gtgtagtcta
     6061 tggccaatcc ccacacaggc tgcatcccaa acgccaggaa gttgtaggcg acggcgtgtc
     6121 tgatcaggtc tcccgtaccg gccatgaaca ggtagcccag cgaataaagc accgttccgc
     6181 tgcccagatc gatcaccaga tgccccaggg acaacacgat cagcatcaat agctccatac
     6241 tctctcctaa tgtgggtttg aattcaagat gagagttgag gggatttctg tcaagccctg
     6301 ctttgctttg gatggtatcg gagaatttcc ttgacggata agcccctcat gctacaataa
     6361 cacaaattgc tatttacggt gccaggcctg agaaacagcc tgatattcag gtagtagatc
     6421 acgtcctggc aacccggaaa ggagaagcta agatggccgc caaaaccata cccttgttct
     6481 cagatgccga tctgaaagga aaaatcgtgt tggtccgcat ggaccacaac gtggtcaaga
     6541 agggcaagat caaagacacc atgcggatag atgcctcttt gcccactctg cgcgaaatca
     6601 tcaaagccgg tgggctacct gtcgtgatga cccacgtggg ccgtccctac gacaaaaaga
     6661 ccggcctcat caccatctct gatgatgaat cggtagctcc aatcgtcacc tatctggatg
     6721 cccagcttgg tctgaaaggc ctcatccctg agctgccggc ctcggaagaa cacggaatca
     6781 ttgatctgga ccccttggcc gcggccatca ccaaactcaa aaacgcagag gtagattatc
     6841 tctatcttcc caataccaga tggtttaaag gcgaggaagc caaggatgcc agcgcggatg
     6901 tctttgccaa agccctggcc tcctacgcgg acctgtatgt caacgacgct tttggctcct
     6961 ggcaggctca cgcctccact tataacatca ccagatatct gccttcttac gcgggcctgc
     7021 tgatgctcaa ggagatcgac agcctgcgcc aggtcttcga tccccagcgt cctctagtcg
     7081 cggtagtggc gggtgcaaag ttcgacacca agatcggccc cctttcctca ctgatcaaaa
     7141 tctgcgatca gctcgtgctc gggggagtga tctacaacgc ttatctggct cataagtatg
     7201 ggcttaagat caaaggcgtg gcagatgagg atgtggccat ggccgccaaa ttcgtctctg
     7261 aggcgggagc atacgcctcc agaatcgtgg agctgccagt catcctcgag tcggatagca
     7321 tcgaagcccg tcaggaaggt gcttatcgtg ctctgcctct caaggacctc aaacccggca
     7381 ccgagctcaa ctacatcctg gacgcggcgc cccagagttt tgaggatcca gccatcaagg
     7441 gggtatttat aaatgcttcc accatctttg tgaacgccgt gatgggctac accgctctct
     7501 ttggagaagg caccgcggcc atgtacagcc tcatagacca aaaccccggg gcccagaagc
     7561 tctttggggg aggagacact atccaggatt acaaagccct gctgccggag ctctttagta
     7621 aagcagccag ggacccacac tactatttct ttacgggcgg aggggccatc ctggacgcca
     7681 tcgaacaggg ctcgccttat ggaatgaagc ccgttgccgc tctgatatca aataaataga
     7741 catacataaa ggagatccat caagtggaat ctgagacaaa aaacggcttg aacctgctgg
     7801 cggaattccc tccccacacc ctggaggaat ggaagcaagc ggtgatcggc agccttaaag
     7861 gcgctgatta tgacaagacg atgctcacca aaacctatga ggggataacc ctcaaaccga
     7921 tttacaccaa ggcagacatg gaaggcaaac cctggctggc atcccttcca ggacaggccc
     7981 cctatttgag aggcgacaat cccgatggct atacagaagg ctcctggata gttgcgcagg
     8041 cgcaggatga aaagtccctg gtcaaactga acaagatcat cctggacgag ctttaccggg
     8101 gcctcaactg cgtgcatctg aagccccatc cagccacttt ggccgccaga ctgccccaca
     8161 aagatgacct tgataaaaga ggcgtaaccc tcaacaatct ggatgacctc aaaacggcgc
     8221 tccgggatgt ggacctcaaa gccgtgccct ttgtgatgtt tggcaacaag agcatgatcc
     8281 tcgtgatggg gatgctgaat gcctgggcca aaagccaggg agtggcgctg cgcgatctgc
     8341 atggcagcat tgcctgtgat cctttccggg atgaagaacc cgattttgat atcctggccc
     8401 agatgacccg ctgggtggac ctcaaagctc cccgcctggg cacaatcctc ctggacgggg
     8461 ccgcctatga agcccaaggg gcctccgcgg tgcaggaact ggcttacagc ctggcctccg
     8521 ctcatcagat catcggcgaa ctcacccagc gcgggctttc catcgagcag cttgctcccc
     8581 gcttcaacct cagtttgtct ctgggctcca atctctttat ggagatcgcc aaaatcagag
     8641 ctgccagaat gctgtggtcg cagctcatca aaagctgggg cgggagtgaa aacgcccagc
     8701 ggatttggat tcatggcgtg accgctgctt tcaacaagag cagctacgat gtctgggtga
     8761 atctgctcag gactaccacg gaaggcttcg cgggagtgat cggaggcgtg gacagccttg
     8821 aaatcacccc gtttgacgat accgtaaggg tcgcggatga gttttcccgc cgcattgcca
     8881 gaaaccagca actcattctg gcggaagaag ctcacttcac caaggtggta gatcctgccg
     8941 ggggatgctg gtacgtcgaa gccctggctg ctgaactgtg tgaaaaggcc tggggtcgtc
     9001 tccaggagat tgaagccgcg ggaggatttc tgagctcgct ccaagccggc accctcgaca
     9061 tggaactgca gactttggcc aaggccagaa tcgcggccgt caatacccgc agagacgtca
     9121 aagtaggcgt caacatgttt gccaaccctg gcgatgaact tctcaaagcc cccagcgccg
     9181 atccagcctg gctggacgag gcaatggaac gttacgcgga gctgaagaag cgcccctcct
     9241 cgggactgga agacagcctc aagctgatca acgaggatag aaacaacgat ttcatggtgg
     9301 atatgatctg cgacgcctgg ctccacaacg ccgacatcga acagatcgcc tccgcgctcg
     9361 gctggaatat caagctcaga cggatggaca aagaacgctc ggtcggcatg atggaagccc
     9421 tccgaatggc cgtggaagcc tcctctatgg atacccgcgt tcagcttctg aacatgggaa
     9481 gcgtcgccca gtataaagcc agggccgatt tctccagcgg atttttccag accgcgggct
     9541 ttaaactgct ggaccagtct ggttttgata ctcccgatct ggccatcgct gctgtagcgg
     9601 aaacccaagt cccggctgtc tgtatctgtt ccaccgacga tctctatgtg gatctggtcc
     9661 cgcggctctg cacaggcctc aagcggcttc ccaaaccgcc tgtcattatc ctggcaggtt
     9721 atccagccga taaagtggaa gattataagg ctgccggagt ggatatcttt atccacatcc
     9781 gcgccaacaa ttatgacacc aataaagagc tcgcgatgag actgggggtg acgctgtgaa
     9841 acctgatctg accaagataa atccggattt caaacccgga accgccgctc ccaaaacctc
     9901 cgcgcccgtc tggaaaacca atgaacagat agaagtcaag gctctcttca cggccgaaga
     9961 catgcaaggc atgcagcacc tggattacct ttccggctta ccgccatttc tgagagggcc
    10021 ctatccttcc atgtttgtgc agcggccctg gacgatccgt caatacgccg gattttccac
    10081 cgccgaagag accaacgcat tctatcgccg caatttagct atggggcaga agggcctttc
    10141 ggtagcattc gaccttgcca ctcaccgcgg ttatgattca gaccatccca gagtggtggg
    10201 agacgtcgga aaagccggtg tggcggtgga ttccatcctc gatatgaaga tcctcttcga
    10261 tcatatcccc ctggaccaga tgagcgtctc catgaccatg aacggcgccg tgatccccat
    10321 catggccttc tatatcgtgg ccgccgaaga gcaaggcgtc aagcctgaac agcttaccgg
    10381 caccatccag aacgacatcc tcaaagaata catggtgcgc aacacctaca tctatcctcc
    10441 cgaagcctcc atgcgcatca ttgcggacat cttttcctac accagcaagc acatgcccaa
    10501 gttcaacagc atcagcattt ccggctatca catgcatgaa gccggcgcaa ctgccgatct
    10561 ggaaatggct tacaccctgg cggacggact ggaatatgcc cgggccggca tcaaagccgg
    10621 catcgatatc gacgtctttg ccccccgcct ttcattcttc tgggccgaag gcatgaacta
    10681 cttcatggag atcgccaagc tcagaggcgc cagagtgctg tgggccaaga tcatcaattc
    10741 cttcaatccc aagaatccca agtctctggc cttgcgcacc cattcacaga cctcgggatg
    10801 gagcctcacg gagcaggacc cctacaacaa cgtggcccgc acctgcatcg aagccctggc
    10861 cgccaccttg ggacacaccc aatccctgca taccaattct ctggatga
//
LOCUS       DAOSFN010000082        10808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5131_length_10808_cov_9.135125, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000082 DAOSFN010000000
VERSION     DAOSFN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5131_length_10808_cov_9.135125"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..9818)
                     /locus_tag="PLO35_06380"
     CDS             complement(192..9818)
                     /locus_tag="PLO35_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79330.1"
                     /translation="MKKPALLLVLLLICGLYAQNTAISEKTVAEISLSDVVEIGNRNA
                     AQMWGEVFPAEPIPYYGPDDEIIAYHLNYSLKGAFPDKDLLKIQCDEALMTNDRDSAS
                     GAGDFGNIVIGAQPYMPVFLQYSQSLSDQFIYGRQLERAALEAFPAGYTEGKIYYLGL
                     VHVWHQISFENTNKYINLRPSVKVISEEEFTNLKAEIPYVWDRNSYDDEWQQFLVNGE
                     TMSRSEVWIEGEDKMPFYEWSYGCTPTSGAMLMAWWDHHKGMGRLISNHYERGDHVEH
                     DRDYHVPTIQQEMASAMGTDDTGTTYLGDVIDAYYQVVENHGYRCVCDGLWAPHHTDT
                     TLFEGIQYQINQGIPCSVGIWGHTIVGVGYLTSPRGVLIHDPNDSVVKFIHKSWLWDY
                     FYVYVYDDSGNNGVFLEITSPNGGTEYANNGPSETLWSGSVEEIAWEGSHPNATATLQ
                     YSLDGGGHWAIITSGTENDGSYRWLVPELDPANFPDGITHQARIRIKIYDNNDELLAA
                     DGSYGNFHIIPNGQFIELQPNVSEGLGVSPYFMKVHPQQDAWGVVSVTSNEPEQLWSV
                     ELFDDQFPSEFENSVIISEAPVRNNYLVIDKRVAEDKTYGLALRQFNPDAVNLTSSVR
                     FSNTNESINLGINELIWYVNGQAKVWDIWLEPGTHYFQMDMVNENADLDFALFKSGGA
                     GFFSRQDALIASDHIGAGVRESFQYTIETAGYYGVCVSSCLNNGTNFTILVSDASIWV
                     GDISGNWYTAQNWLPQFVPGPNHAVQIPSGCTYYPVISASLAPQYTQCKNLVIKAGAM
                     LTVEARNFEVVGDMQTFGTLKLMNSSSEFLVSGSIIWDDGSTLIMGNPDATLLCYRDW
                     TVKAGSYFSGLYRGKVKLISLYDSYIKINSDAFHFPGLVIDKSNGAEAVFSSLSTQDL
                     VVEKDLEIMANSVFKSTSDRSIVIKQSLSNLGSFQFDAGTVKLLGEFGSVSCSAGSYF
                     YNLEINTQRFTTLYSDVHIKGSFFLNSGGFVTNNHTMYVGKNWRNNMGPGGFQKASST
                     VVFNGSGISQCFGELFANLVIDSPDCQLSFAEGESTIDHYTWVNGLLSMRGGNLTIND
                     MPGYGIYGNYLINDGTLNIHQDASQPVDLLGTYFIATGTMNIYGGSSPSSWANADDLS
                     LTMRGGVLDFKDVSILIAPSASTTLDVDLTGGVIRTARDLTVSRDDFNPEGGSFELYG
                     STAASVSITAPSAIPTLIINKDGRNEGSGAAGKSNPERTSYISLLTNLELSSDLIISG
                     GTFDLMGKTVAVGNDLEVYGKLKMVSASDLLQVQGSVYWYEGSSSEISNGVIECYRDW
                     NFWGGTASLTGSNTSVFKGDSNSLIYTVGNQYFANVSFAKTSSTYQENLVTIDSDMQV
                     NGNLTISERSRMILSGSDVLIGGTFIHQGSAAQLVPEGNTVTCNDLDIYGHLMVDGGV
                     FTVQDDFYQRTGSQLSVEAGSFVLSAPYTGTYQSFAGDTVINGGSFEVSNDGIQFGAS
                     SSFLISGGTLKLGWGLRALTPDSFMQTTGTIEFIGTRTASIELAAGNYLHNVVLSKTG
                     SGTLSLGTDTTLNNLTINSGTMLVNHHSLSVNGNVNISGGALNVAFSDDIVYVNGNWT
                     NSRGSAGFIEGNGTVSFMGSGYSLITSNETFNILQIDKSLELGVGAALSANSYEFITG
                     GLSLRDSSILNTPADGFVLPMGSALRCSWGTAPTINLYGNFVDYNMVIDGNNGFYCGN
                     SIANVLGTGNRQIEASVLKFNDLNINLSGANCHINNYSPEFTGNVHLQSGTLSGDYGS
                     IYTFHQGLDTSPGTSLALAGGALTFTGGENAGLSIGGASDYGSLTINKDAGSAVTLGS
                     ALIMPASSTLNVSQGSLQLASQVLSAGGDVNIGNNAEILVNASAQLLMQGSNLLNVNS
                     GGKLSALGSSGQPALISRSGAFYYGLNVYSGGTIAAEHAIFEYMDYAGVNINSGAIVD
                     PAHSFTNCTFQSGSYGGTLLSVNNEQFISITDAVFPSNTWSGMGNVSKNADSGSVSFV
                     NASGAFAGAAFESDPWQRINWNSQNPSIGVNQSSLSFGNVFIPMSEYRSFTITNSGSG
                     SLAGTISLPDDFTANIYRNDTPAANGKADEPQRTSSLDFLALPGTTLEVQVGFLPTQP
                     ISYNSTLVITHNAGGAPINITLSGNGMGAQITVNPTQIIKGILPEGSHTETLSVTDSG
                     NTALSYYGTIEYPTRSRAVIMSESFETAFPPSGWSTAVVQQVGTAGVWSRATSTYHPG
                     GSTPQDGQYLAIFNSWTCSTGNQTRLQSGYLDFSGYSSISLSFWMLHDTAYPASNDRI
                     QVQIFSGQTWQNVGDPIERTQLPEGWRQHTVSLSAYANQNYLQIGLLGISQYGNDLNI
                     DNVVVTGSNPPTGWVFFNGQASSVSGMISPGMTDQHQLNISTNGMEPGTYQAEVHIAS
                     NDPITSVKVVPITVSVGSPGLSVNPSFLNYPLMEVGSESIFGLTLTNSGSIHLNGSIT
                     VPAGYYIQPATRANDVFLTSSQTGGRWSDSEQFILAPGELGSFVVRFAPTAVQVYNGN
                     IVITTDHTGLINVPVTGSAGIVPVVTSGGVSDLSSTSATLYGSITDAGGMTITSKGFY
                     FGSDPDPREYGEILYSESIGNSFTLPLTGLDSGTQYFYCAFANNAMGTGVGEVYLFNT
                     PSPELNVSTTSLPDFGSVTVNTASPAQSFTLDGSAILGDVIISVPTGFRLSVADARTD
                     LPRSDNQIIISPSGGDIAQTTIDVWFQPIEVAPYSGMISISTEGLEDQTITVSGTGVS
                     LPVVTTSAVSDITTSSAVAGGNVTSDGGWQILSKGVCWSLLPNPDISHDHSTDGSGTG
                     AFSSSISPLTPDSSYYLRAYAIYATGTVYGNEITFVTAVEAPASAPDGVTIQINGDYI
                     VLTWDPVPHATSYKVLSSDTPGGAFEEDYRGVFGDCSWTALITDNVRFYQVKAVGGSS
                     SRFVYVPAGTFTMGRTTGTGFADELPSHSVTLSAFYMGKTEVTQKEWTDTMGSNPAAG
                     FGVGDQFPVYNVSWYSVIKYCNLRSLAEGLTPAYSILGSTNPSTWGDVPYEFNPNWDS
                     VICNWSADGYRLPTEAEWEYAARSASNLPDYLYAGSDEIGSVAWYYLNSPSGSKAVAG
                     KTSNALGIYDLSGNINELCWDLAGAYSGDASENPTGPTVGSERIRRGGFWNADATYSR
                     VANRDSSYPFVTNEFLGFRLCRSSQNRQGPLK"
     gene            complement(9856..10263)
                     /locus_tag="PLO35_06385"
     CDS             complement(9856..10263)
                     /locus_tag="PLO35_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79331.1"
                     /translation="MRILICRPNLYLKEELTEYFSGNGWESDLASDPGSIYQSLSQRQ
                     YELILYYVSSLDDFAVIRYINSHYTDLRVVIASDSGFAAGIENVRQGDYTSLGSSGQL
                     GQLRELMTMDAVHPTGDPVEKPITANSLTQSRH"
BASE COUNT         2564 a         2794 c         2762 g         2688 t
ORIGIN      
        1 ccagtgatat gatcctcacg acagaatatg ggaagcagcg atagcggaaa gcgactgccc
       61 ccagacccgg ctgggaggat tgtcccaata gtttagctca gttatccaca ggccagaggc
      121 agccattcag gctgcctctg cttttagtta gatcttttta ttatatcgct ttaagttttc
      181 taccgtccgg attatttaag cgggccttgc ctgttttgag atgatcggca taggcggaag
      241 cccaggaatt cattggtgac aaagggatag gaagaatcgc ggttggcaac ccgactgtag
      301 gtggcatccg cgttccagaa tccgcctctg cggatgcgct cagagcccac ggtggggcct
      361 gtgggatttt cggaagcatc accgctgtaa gcacctgcaa ggtcccagca gagttcgttg
      421 atgtttccgc tcagatcata aatccccaga gcattggatg tttttccggc aacggctttg
      481 ctacctgaag ggctgttcag gtaataccag gccacggagc cgatctcatc tgagccggcg
      541 tacaggtaat ccggaagatt ggaagcgctt ctggcagcat attcccactc ggcttcggtg
      601 ggcaaacggt aaccatctgc ggaccagtta cagatcacac tgtcccagtt aggattgaac
      661 tcgtaaggta catctcccca ggtagatgga ttggtggaac ctaatatgct gtaggccggg
      721 gtaagcccct ctgccagact gcggaggttg cagtatttta tcacgctgta ccaggatacg
      781 ttatagacgg ggaattggtc ccccacgcca aagcccgcgg cgggatttga tcccatggtg
      841 tccgtccatt ctttttgggt aacttcggtc tttcccatgt agaaggcact cagcgtaacg
      901 ctgtgagagg gaagctcatc cgcgaaacca gtgccggtgg tcctgcccat cgtgaaggtc
      961 ccggctggaa catagacaaa cctggaggat gatccgccca ccgctttcac ctggtagaat
     1021 ctaacgttat cagtgataag ggcagtccag ctacagtcac caaagactcc tctgtagtcc
     1081 tcctcaaacg cgcccccggg cgtatctgaa gacaggactt tgtagctggt ggcatggggg
     1141 acgggatccc aggtcaggac tatgtagtct ccattgatct ggatagtgac gccatccgga
     1201 gcggaagcgg gagcttcaac cgcagtgaca aacgtgattt cgttgccata gacggtcccc
     1261 gtagcataga tggcgtaagc tctgagatag tagctggagt ccggcgtaag gggtgaaatg
     1321 ctgctgctga aagcacctgt tccgcttcca tcggtgctgt gatcatggct gatgtcagga
     1381 ttgggcaaga ggctccaaca cacccctttg gagagaatct gccagcctcc gtcgctggtc
     1441 acgttgccac cggccacagc ggaactggtg gtgatatcag ataccgcgct tgttgtcact
     1501 accggcaggg aaaccccggt tccggatacc gtgatggttt ggtcttccag gccttctgta
     1561 gagatgctga tcatcccgga atagggagcc acctcaatgg gctggaacca gacgtctatg
     1621 gtggtctgag caatgtctcc accgctgggg ctgatgatga tctggttatc tgatctggga
     1681 aggtcagtcc tggcatccgc tacgcttaac ctgaatccgg tgggaaccga gatgattaca
     1741 tctcccagta ttgcagagcc atccagtgta aaactctgag ctggtgacgc ggtattgact
     1801 gtcacgctgc caaagtccgg aaggctggtg gtgctcacat tcagctccgg gctgggagtg
     1861 ttgaagagat acacttcgcc cactcccgtt cccatggcgt tgttggcaaa agcgcagtaa
     1921 aagtactgag ttccgctgtc caggcccgta agtgggagag taaagctatt gccgatgctt
     1981 tcggaataga ggatttctcc atattcacgg ggatccggat cgctgccaaa gtaaaaacct
     2041 ttgcttgtga tggtcattcc acccgcgtca gttatagatc cgtacagagt agcggaagtg
     2101 gagcttaaat ctgatactcc gcctgaagtt accactggaa ctatccccgc gctgccagta
     2161 acgggtacat tgatcaagcc ggtatgatcc gtagtgatta cgatgttgcc attatagacc
     2221 tgcacagcgg taggcgcgaa tctcaccaca aaggaaccca actcacccgg agccaagata
     2281 aactgctcgc tgtctgacca gcggcctcct gtttgagaac tggtcaggaa tacatcattt
     2341 gctctggtgg ccggctgaat gtagtaaccc gccgggacag ttatggaacc gtttaagtgg
     2401 atgctgccac tatttgtcag agtcaaccca aagatggatt ccgatcccac ttccatgaga
     2461 ggatagttga ggaagctggg gtttacgctc aggccagggc tgcccactga gactgtgatg
     2521 ggcaccactt tgacggatgt tatcggatca tttgaggcaa tatggacctc agcttgatag
     2581 gtgcctggct ccatgccatt ggtgctgata ttcagctgat gctggtccgt cattccggga
     2641 gaaatcatcc cggatacact gcttgcctgc ccgttaaaga atacccagcc agtgggagga
     2701 ttgctgccgg tgaccacaac attatcgata ttgaggtcat tgccatactg gctgatgcct
     2761 aagaggccaa tctgcaggta gttttggtta gcataggcgg acagactcac agtatgctgc
     2821 cgccagccct caggtagttg ggtcctctcg atggggtctc ccacattttg ccaggtctgt
     2881 ccagaaaata tctgcacctg gattctgtcg ttgctggcag gataagcggt atcgtgaagc
     2941 atccagaagc tgaggctgat actggagtag ccgctaaaat ccaggtatcc tgattgcagg
     3001 cgggtttgat tgcccgtgga gcaggtccag gagttgaaga tggccagata ctggccatcc
     3061 tgaggagtgg accctccggg atgataggtg ctggtagcac gactccacac ccctgccgtc
     3121 cctacctgct gcactactgc agtactccat cctgaggggg gaaaggcggt ttcaaagctc
     3181 tcggacataa ttacagcacg ggagcgggtg ggatattcga ttgtgccata atagctcagg
     3241 gcagtatttc cgctgtctgt gacagatagt gtttctgtat ggctgccttc tggcaggata
     3301 cccttgatga tctgggtggg gttcaccgtt atctgtgctc ccataccatt tccggacagg
     3361 gtaatattga taggtgctcc gccggcatta tgagtaatca ccagggtaga gttgtagctt
     3421 ataggctgag tgggcagaaa tcccacctgc acctcaagcg tggtaccagg cagcgccaga
     3481 aagtccaggc tgctggtgcg ctggggctca tcagcttttc cgtttgcggc tggggtatca
     3541 ttcctgtaga tattcgcggt aaaatcatcc ggaaggctga tggtgccggc cagagaaccg
     3601 gaaccgctgt tggtgatggt gaaagagcgg tattcactca tcggaataaa cacattgcca
     3661 aagctgagac tgctctggtt gaccccgatg gaaggattct ggctgttcca gttgatccgc
     3721 tgccagggat cgctttcaaa ggcggcaccg gcaaaggctc cgctggcatt cacgaaactc
     3781 acagaaccgg aatcggcatt cttgctcacg ttacccatcc cagaccaggt attggagggg
     3841 aagacggcat ccgtaatgct gatgaactgc tcgttgttga cgctcagcag cgttccgcca
     3901 tagctgccac tttggaatgt gcagtttgtg aagctgtggg cagggtccac gatcgcaccg
     3961 gaattgatat tgactcccgc gtaatccatg tattcaaaga tggcatgttc agccgctatg
     4021 gtccctcccg aatacacgtt cagtccatag taaaaagcac cgcttctgga gatcagggca
     4081 ggctgtccac tgcttccaag ggccgagagc ttgccgccgc tgtttacatt cagcagattg
     4141 ctaccctgca tcagaagctg ggcactggca tttaccagga tttccgcatt attgccaatg
     4201 tttacatctc cgccggcgct gagcacctgg gaagcaagct gcaggcttcc ctgggaaacg
     4261 ttaagagtgg atgaagccgg cataatcaga gcagagccaa gggtaactgc actgccggca
     4321 tctttgttta tggtcagact cccatagtca gacgctccac ctatggaaag cccggcattt
     4381 tcaccccctg taaaagtaag agcacctcca gccagagcaa gcgaggttcc agggctggtg
     4441 tccaggcctt gatggaaagt gtagatactg ccatagtcgc cactcagggt tccgctctgc
     4501 agatgcacat tcccggtgaa ttcgggtgag taattgttta tgtggcaatt agctccgctg
     4561 aggttaatgt tcaagtcatt gaacttcagg acggaggctt ctatctgtct gttgccggta
     4621 cccagaacat tggcaattga gtttccgcag taaaatccat tattcccatc tatcaccatg
     4681 ttgtaatcca caaagttccc atagagattg atggtgggag ctgttcccca ggagcatcgc
     4741 agcgcgctgc ccattggcaa cacaaatcca tcggcaggtg tattgaggat ggagctatcc
     4801 cgcagactca accctccggt gataaactca taagagttgg cgcttaaggc agcccccacc
     4861 cccaattcca gggacttgtc aatttgcagg atgttaaagg tctcattgct ggtaatcaag
     4921 gagtatccac tacccataaa ggaaaccgtc ccgttgcctt caataaaccc agcgctgccc
     4981 ctgctgtttg tccaattgcc attcacatag actatgtcat cagaaaacgc gacattcaaa
     5041 gcaccgccgc tgatattgac gttgccgttt acactcaggc tgtggtggtt tacaagcatg
     5101 gtcccgctgt tgatggtcag gttgttcagg gtagtgtcgg ttcccaggct tagggtaccc
     5161 gatccggttt ttgataagac aacattgtgc agatagttac ctgccgccag ttctatggag
     5221 gcagtcctgg tgccgataaa ttctatcgtg ccggttgtct gcataaaaga atctggtgtc
     5281 agggcccgta agccccagcc aagctttaaa gtgccaccac ttatcaaaaa gctgctggag
     5341 gcaccaaatt ggattccgtc attgcttacc tcaaagcttc ccccgttgat cacggtatcg
     5401 ccggcaaagc tttgatatgt acctgtgtaa ggagcactca ggacaaaact tcccgcttcc
     5461 acactaagct gagagccggt tctctgataa aaatcatcct gcacggtaaa cacaccgcca
     5521 tccaccatca gatgcccata aatatccaaa tcattgcagg tgacggtgtt tccctctggg
     5581 accaattgcg cggcagagcc ttgatggata aaggtcccgc cgattagcac atcagagccg
     5641 gaaaggatca ttcggctgcg ttcactgata gtcaggttgc cattgacctg catgtcggaa
     5701 tcaatggtga cgaggttttc ctgataggtg ctgctggttt tggcaaagct tacattggca
     5761 aaatactgat tgcctacggt gtagatgagg gaattactgt ctcctttgaa aacgctggtg
     5821 ttgctgcccg ttagcgatgc cgttcctccc cagaaattcc aatcccggta acattctatc
     5881 acaccattgc tgatctcaga gctggaacct tcgtaccaat acaccgagcc ttgcacctgg
     5941 agcaaatcac tggccgagac catcttcagc tttccataca cttccaggtc gttacccacc
     6001 gccacggttt ttcccatcag atcaaaggtt ccgccgctga tgatcagatc actggataac
     6061 tccagattgg tgaggaggct gatatagctt gtgcgctccg gatttgattt ccctgccgcc
     6121 ccggaaccct cattgcggcc atccttgttg atgatcagcg tggggatagc gctgggagca
     6181 gtgatggaaa cagacgccgc ggtgctgcca tagagctcga aactaccacc ctccggatta
     6241 aaatcatcac ggctcacggt tagatctctg gccgtacgga tcactccgcc ggtgagatcg
     6301 acatccaaag tggtgctggc cgatggagct atcaggatac tcacgtcttt gaagtccagc
     6361 actccgcccc gcatggtcag ggacaaatca tccgcattgg cccaggagct tggcgatgaa
     6421 cctccataga tattcattgt cccagtggca atgaaatatg tccccagcaa atcaaccggt
     6481 tgactggcat cctgatggat gttcagagtc ccgtcgttta tcaagtaatt gccatagatt
     6541 ccatagcctg gcatatcgtt tatggtaagg ttgccacccc tcatgctcaa taatccattc
     6601 acccaggtat aatggtctat ggtggattct ccctccgcga aactaagctg acaatcggga
     6661 ctgtcgatta ccagattggc aaacagctct ccaaagcact gagaaatccc gctaccattg
     6721 aacacaaccg tgctggaggc tttctgaaaa cctccgggtc ccatattgtt tctccagttt
     6781 ttccccacat acatagtgtg gttgttggtg acaaatccgc cgctgttgag gaaaaaacta
     6841 cccttgatgt gcacatccga atagagcgtg gtgaagcgct gggtattgat ctccaggtta
     6901 taaaagtagc tgccggcaga gcaggaaaca ctgccaaact ccccaagcag cttcactgtg
     6961 cccgcgtcaa actggaagct gccaagattg ctgaggcttt gcttgatgac tatgctgcgg
     7021 tcggatgttg acttgaacac agagttagcc attatctcca ggtccttttc caccaccagg
     7081 tcctgggtcg ataaggagct aaagacggcc tctgctccat ttgacttatc gatcacgagg
     7141 cccgggaaat ggaaggcatc ggaattgatc ttgatgtaac tgtcatatag tgatatcagc
     7201 ttcaccttgc ctctgtagag accggagaag taggagccgg ctttgacggt ccaatcccga
     7261 tagcaaagca gggtggcatc ggggttgccc ataatcaagg tggaaccgtc atcccagata
     7321 atgctgccgg acaccagaaa ttcactgctg ctgttcatca gtttcagggt gccaaaggtt
     7381 tgcatgtcac ccaccacctc aaagttcctg gcttccacag tgagcatcgc tccggctttg
     7441 atcaccaggt ttttacactg agtatattgg ggtgccagag acgcggaaat cacaggatag
     7501 taggtgcagc cagagggtat ctggactgcg tgattggggc ccgggacaaa ctggggcagc
     7561 cagttttgag ctgtgtacca gttaccggat atgtcgccca cccagataga agcgtcgctt
     7621 accaggatag tgaaattggt gccgttgttc aagcacgagc ttacacaaac accatagtag
     7681 cccgcagttt caatcgtgta ttgaaacgat tcacgaacgc cagcgccgat atgatctgag
     7741 gcgatcaggg catcctgacg actgaaaaat ccagcaccgc cactcttgaa cagagcaaaa
     7801 tcaaggtccg cattctcatt gaccatatcc atctggaaat aatgcgtccc cggctccagc
     7861 catatatccc acacttttgc ttgtccattg acgtaccata tcagctcatt gattcccagg
     7921 ttaatgctct cgttagtgtt tgaaaacctg actgatgatg ttaagttaac cgcatcggga
     7981 ttgaactgcc ggagtgccag gccataggtc ttatcctctg caacccgctt gtctatcaca
     8041 agatagttgt tccgcaccgg agcctcggag atgatcaccg aattctcaaa ttcactcggg
     8101 aattgatcgt caaacaactc cacagaccag agttgctcag gttcattgct ggtgacgctt
     8161 accactcccc aggcatcctg ttgaggatgc actttcataa agtatggact tacgcctagc
     8221 ccctcagaaa catttggttg cagctctatg aattggccat tgggaatgat atggaaattc
     8281 ccatagctac cgtcggccgc tagcagttca tcgttgttat cataaatctt tatcctgatc
     8341 ctggcctgat gggtgatgcc atcgggaaaa ttagcgggat ccaattccgg gaccaaccaa
     8401 cgataggagc catcattctc tgtgccggag gtgatgattg cccaatgccc accaccgtcc
     8461 agactgtatt gcagggtagc ggtggcattg ggatgagatc cctcccaggc gatttcttcg
     8521 acactgccgg accacaaggt ttctgatgga ccattattag catactcagt tccgccattg
     8581 ggagaagtga tttccaggaa tactccgttg ttaccgctgt catcatagac atacacgtaa
     8641 aagtagtccc atagccagga cttgtgaata aacttcacga ctgaatcatt gggatcatga
     8701 atcaagaccc ctctgggaga ggtcaggtag cccaccccga caattgtatg tccccaaatc
     8761 cccaccgagc agggaatacc ctgattgatc tgatactgga ttccttcaaa caaggtggta
     8821 tccgtatgat ggggagccca taagccatca cagacacagc ggtaaccatg gttttccact
     8881 acctggtaat aggcatcgat cacatcgccc agataggtgg tgccagtgtc gtcagtaccc
     8941 attgccgagg ccatttcctg ttgaatggtg ggaacgtgat aatcccggtc gtgctctacg
     9001 tgatccccgc gttcgtaatg gttggagata agcctgccca ttcctttgtg gtggtcccac
     9061 caggccatca gcatcgcgcc tgaggtgggt gtgcaaccgt agctccattc ataaaatggc
     9121 atcttgtctt caccttctat ccacacttcg gatcggctca tcgtttcccc attgaccaag
     9181 aattgctgcc attcatcatc atagctatta cgatcccaca catagggtat ttcagctttg
     9241 agattggtga attcttcttc ggaaatcacc tttacagaag gtctcagatt gatgtattta
     9301 ttggtgtttt caaaagatat ttgatgccac acgtgcacca aacccagata atagattttt
     9361 ccttccgtat atcctgcggg gaatgcttcc agggctgccc tctccagctg gcgcccatag
     9421 ataaactggt ctgaaaggct ctgggagtat tgcaaaaaca ctggcatata gggctgggca
     9481 cctatcacga tgttgccaaa atcacctgcc ccactggcac tatccctgtc attggtcatc
     9541 aacgcttcat cacactggat tttcagcagg tccttatccg gaaaggcccc cttcaatgag
     9601 tagttcagat gataagcgat gatctcgtca tccggtccgt agtatgggat cggctcggcg
     9661 gggaaaacct ctccccacat ctgggccgcg ttgcggttgc ctatttccac cacatcactg
     9721 aggctgattt ccgcgacagt tttctcagaa atagcggtgt tttgggcata caacccacat
     9781 atcagcaata aaacaagtaa caatgctggc tttttcattt tgtccacctt tgattgtttt
     9841 gtatatgtat cttattcaat gtcttgactg cgttagggag ttagcagtta tgggtttttc
     9901 caccggatcg cctgtcggat gaacagcatc catcgtcatc agttcccgca attgacccag
     9961 ttggcctgag gaaccgaggc tggtgtagtc accctgccgc acgttttcaa tgccagccgc
    10021 gaacccggaa tcagacgcga tcaccacccg caaatccgta tagtgagaat tgatgtatct
    10081 gatcacggca aaatcatcca ggctgcttac atagtataaa atcagctcgt attgtctttg
    10141 ggacagagat tgataaatgc ttcccgggtc gctggcaagg tcggattccc agccattacc
    10201 actgaagtat tcagtcagct cttccttcaa atacagattg gggcggcaga ttaatattct
    10261 cattgattag ctctgagcag gataacagca cactccgttc caaatatcta tcctgctata
    10321 tgccagcact ttaaatcagg atagtgattt tcgcgtctgc attttgctct ccggtttcgg
    10381 aggattgagg ttgctttgtc tatggagatc tgcctgccag acagtggctt agcagcaata
    10441 tcctccgttt tcggatactc cactaacgga ggcaggttgc agagctaagc ctgccatgaa
    10501 gcagagattg tttttggcag gaccggtcat gatgacaaac tgctgatatc cctgggccct
    10561 tgctgcttgt gaaacagagg gacatctgtt gcagagtggt gtgtccaagg gtattgaaca
    10621 atgaagaccg ttgcgcatag ccgtaaccct ggcttcagag cttgtgcaaa aataccacct
    10681 tggccgtctt ggagttccaa gcccctaaat agattatacg tttatctcta tacccgacgg
    10741 ggctttggac ttacgtgcag gacacggagg agcaattctc gcttcggtgt actggttgaa
    10801 gtccaagc
//
LOCUS       DAOSFN010000083        10605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5250_length_10605_cov_8.709005, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000083 DAOSFN010000000
VERSION     DAOSFN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5250_length_10605_cov_8.709005"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLO35_06390"
     CDS             complement(<1..407)
                     /locus_tag="PLO35_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH79332.1"
                     /translation="MLRNLVLKNRSYRRFDASRPITAKTLIHLIELARNCPSAANLQS
                     LRYLPVWDEQLLDAVFPHLRWAGYLRYWDGPLATERPTGYIVILCPAETTKFHHTDAG
                     IVAQTILLGAVEQGLGGCMIASVERDQLRTALD"
     gene            complement(414..1760)
                     /locus_tag="PLO35_06395"
     CDS             complement(414..1760)
                     /locus_tag="PLO35_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOH79333.1"
                     /translation="MPAETQVLIVDDNRDFLDMLRRKLVSRGYKVMTAEDANKALAIL
                     NALPVDILLTDYKMPITNGLELIRHIRLHYSSVSVIMLTGYPTIEGAVEAVKLGAEEY
                     LSKPFTDDELMRALDSVRERIKARALTSKEPVLPSNPYGLIGESEEMQKVYAAIAKSC
                     TNSATVLIEGESGTGKELVARAIHYHSPRERSPFVPVNCGAIPETLLESELFGFMRGS
                     FTGAHETRAGHFITADGGTIFLDEIGEMPLSMQVKLLRVLQDRQVYMIGAKTPRKVNV
                     RVIAATNKDLQLLLRQGSFREDLYYRLNVLPIRIPPLRERSGDIMPLLSFFLNKYAVE
                     NTLPVPSLDPEALRMMNDYHWPGNVREFENLIYRLVILSEGGTIKAQDLPDYMKFSLP
                     HKTNLTRSLAELETEYIKAVVASVDGNKSRAAQILGISRKTLHLKLSAIESSSEKE"
     gene            complement(1750..2901)
                     /locus_tag="PLO35_06400"
     CDS             complement(1750..2901)
                     /locus_tag="PLO35_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79334.1"
                     /translation="MNKEPITSALSEADYVSALRERIKELSCLYSIADLANRPGLELE
                     NLLQQVVEVIPPSMMYPDQAMARLIIDGVSYLAPGYTESGHFIKAGIVVESSPRGQIE
                     VHYPDSEEQLEFLPEEQQLLIAVANQVALLIERKHIEESNSRLQEQLRHADRLATLGQ
                     LSAGIAHEINEPLSTILGYAQLIKEDGQLKSDSASDLGKIISATLHAREVVRKLMLFS
                     RQMPPRLEAIDLNRLIEDGFYFLESRCSHQGVRIVRDLALDLPPLMADPGQIHQVLVN
                     LTVNAMQALPEGGTITIRTLLKGKNVEISVSDNGIGMDSDLLQKIFIPFFTTKEIDQG
                     TGLGLAVVHGIVTTHNGKIKVESKPGRGSVFRVSLPVKPEAAKGGKDAG"
     gene            complement(2964..4295)
                     /gene="gdhA"
                     /locus_tag="PLO35_06405"
     CDS             complement(2964..4295)
                     /gene="gdhA"
                     /locus_tag="PLO35_06405"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOH79335.1"
                     /translation="MTMHEFLHQVSAKNADQPEFIQAVSEVVESIWDVYDSNPQYRKA
                     KILDRIVEPERVIIFRVPWQTDSGEVMVNRGYRVEFNSAIGPYKGGMRFHPSVNLGIL
                     KFLGFEQIFKNSLTTLPMGGGKGGSDFNPRGRSDEEVQRFCHSFMLELFRHIGPNTDV
                     PAGDIGVGKREIGYMFGMYKKITNEVTGVFTGKGLEWGGSLIRPEATGYGTVYFAEEM
                     LKTRGLGFEGKTVVLSGSGNVSQFAIQKVNQLGGKVVTASDSDGFIHDPDGIKGEKWD
                     YLMELKNVKRGRISEYAEEFGVKYYPGARPWGIPCDIALPCATQNEINGEEARTLVKN
                     GCICVSEGANMPTTPEGVEVVLEAKILYGPGKAANAGGVATSGLEMTQNSMRISWSRE
                     EVDAKLHGIMIDIHSQCVLHGKDGNFVNYVKGANVAGFIKVANAMMDQGLI"
     assembly_gap    4339..4438
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4439..7374)
                     /locus_tag="PLO35_06410"
     CDS             complement(<4439..7374)
                     /locus_tag="PLO35_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOH79336.1"
                     /translation="MIASNYWSSETQKLEKVMPHRVQHILLIASLYDSFVFEVDGFLA
                     EQVATDFYLLNLSTQPSIFHASSKQSALSLLELEQIDIAIIHLASMRSIALELISGIK
                     EAAPALPVFILMGAPQDLMFVENHTDMLGSVEDIFYWNGDSKLFLAIIKQWEERRNIA
                     LDCQVLPIPVILVVETFIPYYSQFLPLFYEQIMQLNQAVIRAEHQDINKTLYMNARPR
                     VLLLHDLRSAIGAYETYADNIVGIISNVNYNYLGEFDRDAGLKLLQYVRKRNPTLPFL
                     LQSFNPMYRDIVTSNEGEFLYKDLPGLKAQLRRWLRDEIGFGRFIFRTPDQKIVAEAQ
                     TLIGFAHQLEVIPDESLLYHYRYRHIVNWLRTHAELALADYIGRFESIDQVVILREKL
                     VEAFQSLIAYRRREKIQDYTRESDFAINLVYKIGEDSIGGKGRGLAFLNVVLNRYSNI
                     SEKYPEVEISVPVAAVLATGVFDRFVAHNPRLMDHEAMEDMSDEEIDAMFLGCPFPND
                     IIATLQSVLSKTDFPLAVRSSSVLEDSISNPFAGVFRTYLIPNSHANSEIRLGQMIQA
                     IKLVYSSMYLKSARVYRESLRIPSREEKMAIIIQKVAGSVHGEMFYPLLSGVAQSYNY
                     YPGTNMKHEDGVVTLSTGLGKTAVERERTFAFCPRYPNRDMFRAIDIVEGAQRRFYAI
                     TRDKMDFDLRGGEAASLERVRITQAMLEGDLALLGSVWDYENKEFLSGKYIKGPRVIT
                     YRNLLHYNQYPLAGMIADFLNLGREVLGSEIEMEFAFDVNQKNGRASFYLLQIRPISV
                     KVRQYSQPLEKFLEKREDLMLFSSYALGSDLEHSIDTVVYLKPEQFNVVDTEAMAMEL
                     EAINQTMRESNTRYVLIGPGRWGTSDRFLGIPVNWSQICNAQVIVEVVLPNMSIEASH
                     GSHFFHNLFSMNVGYLTVWDRAVNDWMDWDWLDELKPVSEGKYFQVKKTPRVLKILFD
                     GKNAVI"
     gene            complement(7507..10473)
                     /locus_tag="PLO35_06415"
     CDS             complement(7507..10473)
                     /locus_tag="PLO35_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOH79337.1"
                     /translation="MKMEELNYYYNKFKFGEDIFHQLMQRRVRDILLVSTFYDAFIFE
                     QDGRLSEQIFGEYRQLNLTTAPRITSVPTGADALEKLKERSYDLVITMMRIGEIGPFQ
                     LAKKIKETQPDLPVLLLLNVASDYIIWENHAEEKQYIDAVFLWNGDTKLFLAMVKNIE
                     DNFNVEFDTAHGLVRVILLVEDSIHYYSLFLPSLYSVIMKQTQKLIEEEVSDINKRLR
                     MRIRPKVLLAHDYDEAMRLYKKYREYLLCVISDVRYKIGEEVDPIAGIKLISSIKKDN
                     PELPMILQSSEDENAAKATELGVSFLNKNSKHLFGDLRNYMVYNLGFGNFIFRDSNGV
                     VIDEAANIAEFEDKILHVPEDSILFHSRFNHFSNWLIAHGEVQIAKKIRPMKVEDFAS
                     RDELRHYLHHIFRTVRIDKNRGKIINFDAVSLSEMNQIIRLTEGSLGGKGRGLAFLNA
                     LLCTMEFEKKFENISISLPSTAIIGTNEFDRFVESNNIVEKVQGKTDEEIDAIFISGK
                     LSEQLVNRLEIYLDHVSYPIAVRSSSLLEDSQAQPLAGVYRTFMLPNNHVDKMFRLRQ
                     LMDAIKLVFSSVYLSDARNFLEALNFKAEEEKMAVIIQETVGSVKGEHYYYPHISGVA
                     QSYNFYPTSYMQHTDGIANIALGLGKSVVEGKLNHRFCPRYPETEMLPQQEMVRSSQK
                     EFYALDLRMNDFDLTKGEEITLVKLNLRQAQGHDVLKYVASVWDYENYRLTDNLNEEG
                     MKVLTFAGILKYHHFPLAKIVEELLEIGEIALGIPVEIEFAVNLEHDPRYNHRPTFYI
                     LQIRPLSVNTDAYRIDTEKLDKHNLLMYTEHGMGNGVIQDLTDIIYLDPNRFDKTNTP
                     AMQAEIERLNEKMARLGKRYILIGPGRWGSRDRFLGIPVRWPQINRAKVILETGLRDF
                     IVEASQGTHFFHNLVAMNVGYFTIPFVSATDYVDMEWLLAQPVTDRGDYFAHVEFDHP
                     LIVRMDGKTGLAVIEK"
BASE COUNT         2231 a         2805 c         2909 g         2560 t
ORIGIN      
        1 atatccagcg ccgtccggag ttgatcacgt tcaacggagg cgatcatgca gcctcccaag
       61 ccctgctcga cggctcccaa caagatggtc tgagccacaa tcccggcatc tgtatgatgg
      121 aatttggtgg tctcagccgg acagaggatg acgatatagc cggtgggacg ttctgtggca
      181 agcggcccgt cccagtatct gagataccca gcccagcgga gatgcggaaa tacggcatcc
      241 agaagctgtt catcccacac aggtaaataa cgcagggatt ggaggttagc ggcactgggg
      301 caattgcgtg ccagttcgat aagatggatc agggtcttgg cagtgatggg tcgcgaggcg
      361 tcaaatctgc ggtagctgcg attcttgagc accaggttcc tgagcatggt ttcttactcc
      421 ttttctgagc ttgattctat ggctgaaagc ttgagatgca gtgttttacg gctgatgccg
      481 aggatttggg cggcgcgtga tttgttgcca tccacgctgg ccactacggc cttgatgtat
      541 tcggtctcca gctcagccag agagcgggtg agattggtct tatgaggcag tgaaaacttc
      601 atatagtctg gcaggtcctg ggctttaatg gtcccgccct cactgaggat caccaagcgg
      661 tagatgagat tctcaaactc gcgcacgttt ccaggccagt ggtagtcgtt catcatgcgc
      721 agcgcttcag gatccagaga ggggactggc aaagtgtttt ccacggcgta tttattcagg
      781 aaaaagctca ggagcggcat gatatcacca ctccgctccc tgagaggcgg aatcctgatg
      841 ggcagcacgt tgagcctgta atataggtct tcgcgaaagc tgccttgcct caggagcagt
      901 tgcaggtcct tattggtggc agcgatgacg cgcacgttca ctttgcgggg cgttttagcg
      961 ccgatcatat atacctggcg atcctgcagc acccgtaaga gcttgacctg catggagagc
     1021 ggcatttctc ctatctcgtc caaaaagatc gtacccccgt cggcggtgat aaagtggccg
     1081 gccctggttt cgtgagcgcc tgtgaacgaa cctcgcataa agccaaagag ttccgattca
     1141 agcagggtct caggaatagc gccgcagttg actggcacaa aaggagatcg ctcccggggt
     1201 gagtggtagt ggatggcgcg ggccaccagt tccttgccag taccgctctc cccttcgatg
     1261 aggacggtgg cgctgttggt gcaggatttg gcgatggcgg catagacttt ttgcatctct
     1321 tccgattcac cgatcagacc atagggattg gacggcaaaa caggttcctt gcttgtcagg
     1381 gcgcgagctt tgatcctctc cctcacgctg tccagggccc gcatgagttc atcatcggta
     1441 aagggcttgc tcagatattc ctcggcaccc agtttgaccg cttccacggc gccttcgata
     1501 gtgggatacc cggtcagcat aatcactgat actgagctgt agtgcaaacg aatatggcgg
     1561 atcagttcca aaccattggt gatcggcatc ttataatcgg tgaggaggat gtcgacgggc
     1621 agggcgttga ggatcgccag cgctttgttg gcatcctctg ccgtcatgac tttataacct
     1681 ctgctcacca atttgcggcg cagcatatcc agaaaatcac ggttgtcgtc gacgatcagg
     1741 acttgggtct cagccggcat ctttgccccc tttagccgct tctggcttca cagggaggct
     1801 gactctgaac accgaacctc tgcctggttt gctctccact ttgatcttgc cgttgtgagt
     1861 ggtaacgatg ccgtggacca ccgcgagtcc caagccggtg ccctgatcaa tctctttggt
     1921 ggtgaagaag ggaataaaaa tcttttgcag caggtcgctg tccataccga tcccgttgtc
     1981 ggacacgctg atttcgacgt ttttgccctt caggagggtg cgaatggtga tggtgccgcc
     2041 ctccggaagc gcttgcatgg cgttaacagt gaggtttacc aatacctggt ggatttgtcc
     2101 cggatcagcc atcaagggag gcaaatccag ggccagatcg cggacgatcc tgaccccctg
     2161 atgggaacag cggctttcca ggaaataaaa gccatcctca atcagccggt tgagatcgat
     2221 agcctcgaga cggggaggca tctgccgtga aaacagcatc agctttcgca ccacttctct
     2281 ggcgtgcaga gtagctgaga tgatcttgcc gagatcgctg gcagagtctg attttagctg
     2341 gccgtcctct ttgatcaatt gcgcgtagcc caggatggtg ctgagaggtt cgttgatctc
     2401 gtgagcgatg ccggctgaaa gctgtcccag cgtggcaagg cggtcggcat gacgcagctg
     2461 ctcctgaagc ctggaattgg attcttcgat gtgtttgcgc tcgatcagca gggccacctg
     2521 attggcaacg gcaatgagca gctgctgttc ttccggcaga aattccagct gttcttccga
     2581 atccggatag tgaacttcga tctggccgcg gggactgctt tccaccacga ttcctgcttt
     2641 gataaagtgc ccgctctctg tatatcccgg agccagatag ctcacgccgt cgatgatcag
     2701 cctggccatc gcctgatcgg gatacatcat ggagggcggg ataacctcaa ccacttgctg
     2761 gagaaggttt tccagttcca atcctggccg gttggcgaga tctgcaatgc tgtaaagaca
     2821 gctcaattcc ttgatccgct ctcgcagcgc gctcacataa tctgcctccg acaatgcgga
     2881 agtaataggt tccttgttca tgtcttcctt cgagaaaaag gggccggcgc gaagcaggcc
     2941 cccatcgatt gattaggttg tgatcagata agtccctggt ccatcatggc attggctact
     3001 ttgataaagc ctgccacgtt ggcacccttg acgtaattga caaagttgcc atccttgccg
     3061 tggaggacgc actggctgtg gatgtcgatc atgatgccgt gaagtttggc gtccacttcc
     3121 tcgcggctcc agctgatgcg catgctgttt tgggtcattt ccagaccgct ggtagcaacc
     3181 ccaccggcat tggcggcttt gccgggaccg tagaggatct tggcttccag caccacttcg
     3241 acgccttcag gagtggtggg catgttggca ccctcagata cgcagatgca gccgtttttg
     3301 actagagtgc gggcttcttc gccgttgatc tcgttttggg tggcgcaggg cagagcgatg
     3361 tcgcaaggga ttccccaagg acgcgctcct ggatagtatt ttaccccaaa ttcctcggcg
     3421 tattcgctga tgcggccacg tttgacgttc ttgagttcca tcaggtaatc ccacttctcg
     3481 cctttgatgc catccggatc gtggatgaag ccgtcggaat cggaggctgt gaccaccttg
     3541 ccgcccagtt ggttgacctt ttggatggca aactgggaga cgtttccgct gccggaaagc
     3601 acgactgtct tgccttcgaa gcccagaccg cgggtcttga gcatctcttc ggcaaaatag
     3661 acggtcccat agcccgtggc ttcaggacgg atgaggctgc cgccccattc gaggccctta
     3721 ccagtaaaga caccggttac ctcgttggtg atctttttgt acatgccaaa catatagccg
     3781 atctcgcgtt tgcccacacc gatgtcgcca gcggggacgt ccgtgttggg gccaatatgg
     3841 cggaaaagct ccagcataaa gctgtggcag aaacgctgca cttcttcatc ggaacggcca
     3901 cggggattaa aatcgctgcc gcccttgcca ccacccatgg gtagagtggt gagactgttt
     3961 ttgaagatct gttcgaaacc caggaacttg aggatgccca gattgacgct gggatggaac
     4021 ctcatacctc ccttgtaggg tccgatggcg ctattgaatt ccacccggta tccgcggttg
     4081 accatcactt cgccgctgtc cgtctgccag ggaaccctga atatgatcac gcgttcaggc
     4141 tccacgatgc gatccaggat cttggccttg cgatattggg gattggagtc atagacgtcc
     4201 caaatggatt ccaccacttc tgagacggcc tggatgaact ccggctggtc ggcatttttc
     4261 gccgatacct ggtgtaagaa ttcgtgcatt gtcatgttca catacctcct gtgatttttt
     4321 atcgatgaca cgagttttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     4441 aatgacggcg ttttttccgt caaacaaaat cttcaatact ctgggagttt tttttacctg
     4501 gaagtatttt ccttcggata caggcttcag ttcatccagc caatcccaat ccatccagtc
     4561 gtttacagcc ctgtcccaca ccgtcaggta gcccacattc atgctgaaaa gattgtggaa
     4621 gaagtgggag ccatgcgagg cttcgatgct catgttgggc agcaccacct ccacgatcac
     4681 ctgggcgttg cagatctggc tccaattcac gggaatcccc aaaaagcgat cgctggtgcc
     4741 ccatcttccc ggtccgatca acacgtaacg cgtgttggac tcgcgcatgg tctggttgat
     4801 ggcctccagt tccatggcca tcgcttcggt atccaccacg ttaaactgct ccggctttag
     4861 atagaccacc gtgtcgatgc tgtgttcaag atcgctgccg agcgcgtagg aagagaacag
     4921 catcaagtct tcgcgctttt ccaggaactt ttccaagggc tgagaatact gcctcacctt
     4981 taccgagatt ggcctgatct gcaagaggta aaagcttgcc ctgccattct tttggttgac
     5041 gtcgaaggca aattccatct cgatctcgct acccagtact tctctgccca gattgagaaa
     5101 gtcggctatc atcccggcaa ggggatactg attgtagtgc aagaggttgc gataggtgat
     5161 caccctgggg cctttgatat acttaccgct gaggaattcc ttgttttcat aatcccacac
     5221 actgcccagc agggcaaggt cgccctccag catagcctgc gtgatcctca cccgctccag
     5281 agacgccgct tccccacccc tgaggtcaaa atccatctta tctctggtga tggcatagaa
     5341 gcgccgctgt gccccctcca cgatatcgat ggcgcgaaac atgtcccggt tgggatagcg
     5401 gggacagaaa gcaaaggtgc gttcccgttc caccgcggtt ttgcccagtc cggtggagag
     5461 tgtcaccacc ccgtcctcat gtttcatgtt ggtgccggga tagtagttgt aagactgcgc
     5521 cactcctgag agcaggggat aaaacatctc gccgtgtacg ctgcccgcca ccttttggat
     5581 gatgatggcc atcttttcct cgcgggatgg gatgcgcagg ctttctctat agacgcgggc
     5641 gcttttcaga tacatggagg aatagaccag cttgatcgcc tggatcatct gtcccagccg
     5701 gatctcggag ttggcgtggc tgttggggat cagataggtg cgaaagactc ccgcgaaggg
     5761 attggaaata ctatcctcca gcaccgagga cgaccgcaca gccagcggga aatccgtctt
     5821 gctgagcacg ctctggaggg tggcgatgat atcgttgggg aaaggacagc ccagaaacat
     5881 ggcgtcgatc tcttcatcgc tcatgtcctc catcgcctca tggtccatca gacggggatt
     5941 gtgggccaca aagcgatcaa aaactcccgt ggccaacacg gccgcgacgg gcacgctgat
     6001 ctccacttcc ggatacttct cgctgatgtt gctgtagcgg ttcagcacca cgttcagaaa
     6061 ggccagtccg cgtcccttgc cgccgatgga gtcttcaccg atcttataga ccagattgat
     6121 ggcaaaatcg ctctctctgg tgtaatcctg gatcttttcc ctgcgtctgt aggcgatcag
     6181 ggactggaag gcctccacca gcttctcacg caggatcacc acctgatcga tggattcgaa
     6241 gcggccgatg taatccgcca gggccagttc cgcgtgggta cgcagccagt tcactatatg
     6301 cctgtaccgg tagtgataaa gcagactttc atcaggtatc acttcaagct gatgcgcgaa
     6361 acctatcagg gtctgggcct ctgccacaat cttctggtct ggagtacgaa agatgaatct
     6421 gccaaagccg atctcgtccc tcagccagcg gcgcagttgg gctttgaggc caggcaggtc
     6481 tttgtagaga aattcacctt cattgctggt gacgatatcc cggtacatgg ggttgaagct
     6541 ctgcagcagg aaaggcaagg tggggttgcg tttacgcaca tattggagca gtttcagccc
     6601 ggcatcccga tcaaactcgc ccaggtagtt gtagttgacg ttgctgatga tgcccacgat
     6661 gttgtccgcg taggtctcat aagcccctat ggcgcttcta aggtcgtgga gcagcaacac
     6721 tctgggcctg gcgttcatat atagcgtctt gttgatgtcc tgatgctcgg cgcggatcac
     6781 ggcctgattg agctgcatga tctgctcgta aaagagcggt aaaaactgcg agtaataagg
     6841 gatgaaggtc tccactacca ggatgacggg aatcggcagc acctgacagt caagagcgat
     6901 gtttctgcgt tcctcccact gcttgatgat ggccagaaac agcttggaat ccccgttcca
     6961 atagaagata tcttccacgc tgcccagcat atccgtgtgg ttttccacaa acatcaggtc
     7021 ttgaggggct cccatcagga taaagacagg cagtgcggga gccgcttcct tgatcccgct
     7081 gatcagttcc agagcgatag accgcatcga agccaggtga atgatggcaa tgtctatctg
     7141 ctccagttcc agcaaactaa gagcgctttg tttggaggac gcgtggaaga tgctgggctg
     7201 ggtgctgaga ttgagcagat aaaaatccgt ggccacctgc tccgccagaa atccatccac
     7261 ttcaaacacg aagctgtcat acagggaggc gatcagcagg atgtgctgca ccctgtgagg
     7321 catcactttt tcaagctttt gggtctcgct ggaccagtag ttagaagcta tcatgggtga
     7381 gagctctgcg agctggtgag agccgttcgg gctggggaga acatcgtcga tttccaaacc
     7441 ggcgcgtgac tgtaacgcat aacgcataac gcttaacgct taacgcgtaa ccaaacatca
     7501 ctttcatcat ttttcaatca cggccaggcc ggtcttgcca tccatcctca cgatcagggg
     7561 atggtcaaac tccacatgcg caaagtagtc tcccctatcc gtaaccggct gggccagaag
     7621 ccattccata tccacatagt ccgtggcgga aacaaaggga atagtgaagt agcccacgtt
     7681 catagccacc agattgtgga agaagtgggt gccctgggaa gcctccacga tgaagtcccg
     7741 caggccggtt tcgaggatca ccttggcgcg gttgatctga ggccagcgca ccgggatgcc
     7801 caaaaaacgg tcccgggagc cccaacgtcc tggcccgatc aggatatagc gttttcccag
     7861 cctcgccatc ttttcgttca ggcgttcgat ctcagcctgc atggcgggcg tgttggtctt
     7921 gtcaaaacga ttggggtcca gatagataat atcggtgagg tcctgaatca cgccgttgcc
     7981 catcccgtgt tcggtataca tcaggaggtt gtgtttatcc agcttttccg tgtcgatgcg
     8041 gtaagcgtcg gtattcacgg agagcggcct gatctgcagg atatagaagg tgggcctgtg
     8101 gttgtagcgg gggtcgtgtt ccaggttgac ggcaaattcg atttcgactg gaatacccaa
     8161 cgcgatctcc ccgatttcca gcagttcttc cacgattttc gccagcggga agtggtgata
     8221 cttgaggatc cccgcgaagg tcagcacctt catgccttct tcgttgaggt tgtccgtgag
     8281 gcgatagttt tcgtaatccc acaccgaggc cacatatttg agcacgtcat ggccctgcgc
     8341 ctgcctgaga ttgagcttca ccagcgtgat ttcctcgcct ttggtgaggt caaagtcgtt
     8401 catacgcaga tccagcgcgt aaaactcttt ttgggaggag cgcaccatct cctgctgggg
     8461 cagcatttcc gtctcgggat aacgcggaca gaagcgatgg ttgagcttgc cttccaccac
     8521 cgattttccc aggccgaggg cgatgttggc gatcccgtcc gtgtgttgca tatagctggt
     8581 gggatagaag ttgtaggatt gcgccacgcc ggagatgtgg ggatagtaat agtgctcgcc
     8641 cttcacgctg cccaccgtct cctggatgat caccgccatt ttctcttctt cggctttgaa
     8701 gttcaaggct tccaaaaagt tgcgggcgtc gctcagatac accgaggaga acaccagctt
     8761 gatggcgtcc atcagttggc gcaggcggaa catcttgtcc acgtgattgt tgggcagcat
     8821 aaaggtgcga tagactcccg ccagaggctg ggcctgggaa tcctccaaca gactggaaga
     8881 gcgtaccgcg atgggatagg acacatggtc cagatagatc tcaaggcggt tcaccagctg
     8941 ctcggagagc tttccgctga tgaagatggc gtcgatttcc tcatccgtct tgccctgcac
     9001 cttttccacg atgttgttgc tttccacaaa gcggtcaaac tcgttggtgc cgatgatggc
     9061 cgtggagggc agggaaatgg agatgttttc aaacttcttt tcaaattcca tcgtgcagag
     9121 cagcgcgttc aggaaggcca gtccgcgtcc cttgccgccc agcgagcctt ccgtgagcct
     9181 gatgatctga ttcatctcgc tgagggagac ggcgtcgaag ttgatgatct tgccgcggtt
     9241 tttatcgatg cggacggtgc ggaagatgtg atggagatag tggcgcagct catcccgact
     9301 ggcaaagtcc tccaccttca tcggcctgat tttcttggcg atctgcactt ctccgtgggc
     9361 gatcagccag tttgagaagt ggttgaagcg gctgtggaag aggatgctgt cttccggcac
     9421 gtgcaggatt ttatcctcaa actcggcgat attggccgcc tcgtcgatca ccactccgtt
     9481 gctgtcgcgg aagatgaaat tgccaaagcc gaggttgtaa accatatagt tgcgcaaatc
     9541 tccaaacaga tgcttggagt tcttgttcaa aaagctcacg cccagctcgg tagctttggc
     9601 ggcgttttcg tcctcggaac tctgcaggat catcgggagc tcgggattgt cctttttgat
     9661 gctggagatc agcttgatac cggcgatggg gtccacctct tccccgatct tgtagcgcac
     9721 gtccgagatc acgcagagca ggtactcgcg gtatttctta tagagccgca tcgcttcgtc
     9781 ataatcgtgg gccagcaaca ccttgggccg gattcgcatc ctgagccgct tgttgatgtc
     9841 actcacctcc tcctcgatca gcttctgcgt ctgcttcatg atcacgctgt agagggatgg
     9901 caggaaaagg gaataatagt ggatgctgtc ttccaccagc aggatcacgc gcaccaggcc
     9961 gtgggcggtg tcaaactcca cgttgaagtt gtcctcgata tttttgacca tcgccagaaa
    10021 cagcttggta tcgccgttcc agaggaagac ggcgtcgata tactgctttt cctcggcgtg
    10081 attttcccag atgatatagt ccgaagccac gttcaacagc agcaggacgg gcaggtccgg
    10141 ctgggtttct ttgatctttt tggccagttg aaaggggccg atctcgccta tcctcatcat
    10201 cgtgatcacc aggtcatagc tgcgttcctt gagcttttcc agcgcgtccg ccccggtggg
    10261 aacgctggtg atgcgaggcg cggtggtgag attgagctgg cggtattctc caaagatttg
    10321 ctccgaaaga cggccatcct gttcaaagat aaaggcgtcg taaaaagtgg acaccaacag
    10381 gatgtcgcgc acccggcgct gcatcaactg atggaatatg tcctcgccaa acttgaactt
    10441 attataatag tagttcagct cttccatttt cataaacacg gtcctcgaca catcaaattg
    10501 gttttttggc atcagagcgg gtttggggat ttgtgtcaac agaaaagtta ggttttaggg
    10561 ttttactacg gagatacaga ggtttctttt ttcactacag agcta
//
LOCUS       DAOSFN010000084        10352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5469_length_10352_cov_8.804409, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000084 DAOSFN010000000
VERSION     DAOSFN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10352
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5469_length_10352_cov_8.804409"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..802
                     /locus_tag="PLO35_06420"
     CDS             131..802
                     /locus_tag="PLO35_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOH79338.1"
                     /translation="MNYQAIIFDLDGTLIDSMLLWRRVDAEFLNKRGIEVPGDLFDHL
                     PSGNSFIQTAQYFIDRFRLPDSVESVMSEWTEMVRWHYTHDVSLKPGAGELVSEIAAS
                     NIKIGLGTSNSYELAQTVLSANRIWQHFHCAITGDMHLMGKPWPDIYLKCADALQVPA
                     KNCLAVEDTLTGVQAAKTAGMRTFAIYDEDSQPFWERIKALADEAFMDYRQLGQAVRR
                     ELGIN"
     gene            813..1502
                     /locus_tag="PLO35_06425"
     CDS             813..1502
                     /locus_tag="PLO35_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79339.1"
                     /translation="MAEALYIVIGAYGSGKSEYSIHLARSLKDQGQDVVLADMDVVNP
                     YFRSRDVRDDFTALGIEVIAPDGEFKHADLPMISPRIKGAIENYSRTVVLDVGGDPAG
                     CRTLGRFVDVIKDRGYEMHFVVNTRRPFTSNVEEVLTMQTMLEFSSKLKITHLICNSN
                     LMQFTDTAVVEEGIAILKEVATQSDLIFEIYLALEEYANRVPDGLSGLKRVLMEYTLQ
                     KPWERLCAKGI"
     gene            complement(1509..2519)
                     /locus_tag="PLO35_06430"
     CDS             complement(1509..2519)
                     /locus_tag="PLO35_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOH79340.1"
                     /translation="MSEILITRSRGSIVECVHFGDVAVVGPAGKLIASCGDADKYTYF
                     RSSAKPIQALNLIISGAAERFGINDQELALVCASHYSEDQHLETARSILAKAGLDESY
                     LRCGAARSIKPEIAFAQAEAGIPPQRIKSDCSGKHSGMLATCAHKGYPLDTYLNPEHS
                     LQQELIAIVSEVCDYPVEKIGVGVDGCGVPVFALPIRNMALAYARFAAPETLPERYRS
                     AAIRIFEAMNAYPAMVSGTGGFCTALMQATGGRMIGKVGAMGVYTIGIKTARLGIALK
                     IGDGSPGMAQVAAMHVLRELDLLTEAEYERLESFHRPPVLNDEGLRVGEVRAVFSLKK
                     NG"
     gene            3404..3481
                     /locus_tag="PLO35_06435"
     tRNA            3404..3481
                     /locus_tag="PLO35_06435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3439..3441,aa:Pro,seq:tgg)
     gene            3570..3645
                     /locus_tag="PLO35_06440"
     tRNA            3570..3645
                     /locus_tag="PLO35_06440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3603..3605,aa:Val,seq:tac)
     gene            3667..4638
                     /locus_tag="PLO35_06445"
     CDS             3667..4638
                     /locus_tag="PLO35_06445"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOH79341.1"
                     /translation="MKIAVTGANGFLGSKLVSRLTGYKRQTVALLRPNADRSLLSGDQ
                     EIRELDYSNPASIRAAISDCDVVIHNAGKTRTLRFEEMLDANLGITAKLIEAVNGSSV
                     EHFIYISSQAASRPSMDGEAINESSEPAPVNWYGKSKLWAERAVQSRCQKAWTIVRPV
                     PVYGEGDRDFLSLFRMAASGFSFSLGSQRQILNMIHADQLCDFIQTCIATPAARNELF
                     FASDGQHYTQGEIISMIMDAVGKRPLLKVEIPIALAQPVFQMGELLSIMRGKASLVGR
                     QKYLELSAPHWNCDVSKARRLLGWNPELSIERRMQETWLWYRAQGWL"
     gene            4611..6566
                     /locus_tag="PLO35_06450"
     CDS             4611..6566
                     /locus_tag="PLO35_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79342.1"
                     /translation="MVSRPGLAVILLALSVWLTGLMAETVSPGPSAPMRPVYLSEAPA
                     LELSATDASLPQRVLIRIAVFPDSSYTLLSVEPANLRAEVERYLRQTDLMGYLDDKTP
                     GVKELEIRVSRIEQAAMEATGHTTEPELRQRIDDWIFKQRELDSPGAVWRDWLDPLAS
                     EDLNYRSATNVSGLHTGTDPLRQHGMDLANTPLAKVQRLNWLRSIYSSAVAYDHYDEP
                     YPYEILLTGLNAGMGEFDSRYAKVRAGRNGLLGREGLFYGLDLTVSSGDWLNLSSAQT
                     NIRQSLRIPLGKLGLELDYASFAADQASISLHPSYWRSPLYGLERKGELLWTRLSSPW
                     LDLAYSRSREALKSDVFAFRPLSLMHQLQAAKELQMGKIKMGASWIHAWRENNLLLPL
                     ESYQSEYKDLGAVKLDYQGDKLTVNASAALADLADLMAEGSVAYRFGKLTCSAGLKAD
                     FSDYQPRVAVTDIYTSNAELESVDLRERQRLWMKLDWQHSALKLALTAGRKDYQIALP
                     NSGNTLQYKAAPLFVGLGGVYDLDLGFMRAILRQNLLWTQEQEHTRDDPAIRGFGELE
                     LRRELSYNNAIFAALGYTFHSSYLNLAVQPDVLDFSLIADARFGVRISKLFEIDAGIR
                     NLGDNYIFGVYPIPFSLHASVRWYFVN"
     assembly_gap    6575..6674
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6675..8437)
                     /locus_tag="PLO35_06455"
     CDS             complement(<6675..8437)
                     /locus_tag="PLO35_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79343.1"
                     /translation="MSKRFARGLLKRMNPHLAWIARQWYTQRWFLIIMLFFTLGSSAV
                     SIAYPIVFRRLIDLLRDILTSPQKYPEPMGAVNRVIWMFMAIGLAQLITGFYPAFRGW
                     VNIRFEHLLRMKYFNAITDKDFIFFQKYRTGDIVTRLTDDLSDFPKISWFLCSGIFRA
                     VNSGSMILFSLIVMFSVSTKLTLFSIAPLPLMMVVFYVTSDKLYKNFERNQQAISAIN
                     NQLEMSFSGIRIVKAFVSEEKYNRFFDTALAKRFKTEMSVVKLNAILHLIYEYIDYFA
                     QIGVILFGGYLAVKGEISVGTFFLFYTYLSMMIYPLLDLPQLFVSGKQAFVNIDRLEE
                     MRGFPSLARRQLGTHKVESFDSLRFDQVVCAYPEKDKPVIADCGFQLAKGEKLLILGS
                     TGAGKSTLVNLMLGLLEKRSGRIEVNGIPIEALDITALREIVAYVPQEPVLFSGTIQD
                     NVRFAVSNASDNEYRLAVNSAQLADEIASFPDGDQTKVGQRGIGISGGQKQRVTIARA
                     LIKHPQLLILDDITASLDAENEEKLWQDINTNYPGIAAIVISHRLSTLRYVDKVLYID
                     SSGICHHGTHEELVFNNQEYH"
     gene            complement(8430..10193)
                     /locus_tag="PLO35_06460"
     CDS             complement(8430..10193)
                     /locus_tag="PLO35_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79344.1"
                     /translation="MKEQKQNLSYYFRLLYPYVKQDKGLLFFGLMAMLITSSLQLVDP
                     LILALIVDKSVPAGDMTQTFRYGMIFVGVVLVSGLLSYLQIVLLSRLGIKVITNFKGR
                     VFRHLLILPVEWFNRQSVGELIARVESDSERVKVLFSELSIRIIGNFLFFIGVFVVLF
                     IRQWQVTIWILPAMGTAIVGYYYLIRYLSKFYRLIREKYADITGRITDYVQGMPMIQA
                     LNQQKRVVEDLRMASYDKYKLETRTSFTEYGSQSLFTFIFQVAFIILVISLTAPKIIA
                     GAVTLGTLIIFVQYLNRMIWPLMQISENVMQIQRSFVSLRRILDLTELGTEEELFTGT
                     QTPVFEHDIRFDHVWFAYKDEDWILKDVSFTIPKGKKVALVGASGSGKTTTVSLLCGF
                     YPIQKGAIRIDGVALSDLDFRAWRAKIGLILQDIFLFPGSILENVRVYNDLVPESKVR
                     DAINIVQLDDLIKAQTDGLNTELAERGQNVSQGEKQLISFARALAFDAELIVMDEATA
                     SIDPQTEARIQRSMDSVFESRTAVIVAHRLTSVMDADEILFFSQGEIIHRGTHDELMH
                     KSPEYRKLVELQMLRGEAVNE"
BASE COUNT         2371 a         2732 c         2863 g         2286 t
ORIGIN      
        1 gagcgcaacc ttgccaaggt tgaagtcgcg ggttcgagtc ccgtttcccg ctccatttat
       61 tttatccgga caaaatcgcc ttcttccgaa tctgattgac ggataggccc ccatcagaat
      121 actgcctccc atgaattatc aagctatcat cttcgatctg gatggtaccc tcatcgattc
      181 catgctgctc tggcgccggg tcgatgcgga attcctcaat aaacgcggca tcgaggtgcc
      241 gggtgacctc tttgaccacc taccgtccgg caacagtttt atccaaacgg cccaatactt
      301 tatagaccgt ttccggctgc ccgacagcgt ggaaagcgtc atgagcgaat ggaccgaaat
      361 ggtgcgctgg cactatactc acgacgtttc tctcaagccc ggagcgggtg aactggtttc
      421 agagattgcg gcatcaaaca tcaaaattgg cctcggcacc agcaattctt atgaactggc
      481 ccagaccgtt ctgagcgcca atcgcatctg gcaacacttt cactgcgcca ttaccgggga
      541 catgcatctg atgggtaaac cctggccaga catatattta aaatgcgcgg acgctctgca
      601 ggtcccggct aaaaactgcc tggccgtcga ggataccctc acgggagtcc aggccgccaa
      661 aactgccggg atgcggacct ttgccatcta cgatgaagac agccagcctt tctgggagcg
      721 gatcaaagcc ctggccgacg aggcctttat ggactaccgg caactgggcc aggccgtcag
      781 gcgcgaactg ggtatcaact aaggagcaaa acatggcgga agccttatat atcgtcatcg
      841 gggcctatgg cagcggtaag agtgaatatt ccatccattt ggcgcgcagc ttgaaagacc
      901 agggccagga tgtagtcctg gccgatatgg atgtcgtcaa tccctatttc cgcagccgcg
      961 acgtgcggga tgattttacc gccctgggca tcgaagtcat cgctcccgac ggggagttta
     1021 agcatgccga tctgccgatg atctcgcccc gcatcaaagg cgctatcgaa aactactcca
     1081 ggacagtggt tttggacgtg ggaggcgatc cagccgggtg ccgcaccctg ggcagatttg
     1141 tggatgtcat caaggaccgg ggctatgaga tgcactttgt ggtcaatacc cgccggccct
     1201 tcacatccaa tgtggaagag gtcctcacga tgcagaccat gctggagttt tcatccaaac
     1261 tcaagatcac ccacctgatc tgcaacagca acctcatgca gttcaccgat accgctgtcg
     1321 tggaagaggg catcgccatt ctcaaagagg tcgcgaccca atcagatctg atcttcgaga
     1381 tttacctcgc ccttgaggaa tacgccaatc gcgtccccga tggtctcagc ggcctgaagc
     1441 gcgtcctgat ggaatacacc ctccaaaaac cctgggaacg gctctgcgcc aaaggaatct
     1501 gagtatcctc agccgttctt cttaaggcta aacaccgctc tcacttctcc cacccgcaat
     1561 ccttcatcgt ttagcacggg tggccggtgg aatgattcca accgttcgta ttccgcttct
     1621 gtgagcaggt ccagttccct aagaacatgc atggcagcca cctgggccat tccgggactg
     1681 ccgtcgccga tcttgagagc gatgcccaac ctggcagtct tgatcccgat cgtgtaaacg
     1741 cccatggcgc ctacctttcc gatcattctg ccacccgtgg cctgcatcaa agccgtgcag
     1801 aagcctcccg tgccggaaac catcgctgga taagcgttca tggcctcaaa gatcctgatc
     1861 gcggcgctcc ggtatcgctc tggcagggtt tcgggagcgg caaaacgggc ataagcgagc
     1921 gccatattgc ggatcggtaa tgcaaacacc ggcactccac aaccgtccac tcccacgcct
     1981 atcttttcaa caggataatc gcagacttcc gaaacgatgg cgataagctc ttgctggaga
     2041 gaatgttcag gattcagata cgtgtccagc gggtaacctt tgtgcgcgca ggtggccagc
     2101 atgccgctgt gttttccgct gcagtcgctc ttgatcctct gaggcgggat acctgcttcg
     2161 gcttgggcaa aggcaatttc cggctttatg gacctggccg cgccgcacct cagatagctc
     2221 tcatccagac cggcttttgc cagaatgctc ctggccgtct caaggtgctg gtcctcggag
     2281 taatgcgaag cacagaccag cgccagttcc tggtcattga tgccaaaccg ctccgcggca
     2341 ccggaaatta tcaggttcaa agcctggatc ggtttggcgg aagaccggaa ataggtgtat
     2401 ttatccgcat cgccgcaact ggcgattagc ttgccagcag gaccgactac ggcgacatcg
     2461 ccaaagtgca cgcattccac gatgcttcct ctgcttctgg tgatcaggat ttcggacatc
     2521 tgtcctccca tgtgtaaact tggggcatca atttccgggg ctgaaacggt tgtcaatcat
     2581 aatctttccg ccttctctct gtatccttcc cgcatcctaa tacggactca ttacggactt
     2641 agtccgtaat gagtccgtaa tgactccgta atgctatgcg gaggggaagg ggagattgag
     2701 tggaaacccc tgaagggtat tgaattgagt cgatatttat catgcttgcg gatttgagac
     2761 actcgattac ggattcataa tacccctgag ctgatcaatg ggacggccaa atcaaagact
     2821 tgggtagaag gattgtcaat tcacaagtct gagcccagcg ggcgacacaa tgtcatagcc
     2881 ccccgtgtag cgaagcggaa cggggggacc tgattcagac gattccgaag agctccccgg
     2941 gagcgggaca cgtttgtatg tttcatatcc tgtttttgat aatgctatgt tccacccccg
     3001 aggggttcag acggatggtc gttctgatcc ccccgttccg cttcgctaca cgggggggct
     3061 ataacgttga gccgccctcc gggctggaat tgcggcctgg atggaacaat tggggggcaa
     3121 ttcaatgatt tttctcacat agccccttag ccaaacgtag agtgggttga gacggtaggt
     3181 ggtgtaaacc ttctcaaccg tctcaacagc tgtacagaca gacgccgctt gagccatttt
     3241 ccactttcca tcagcacaaa agcgagcatc ggtactttca accttctcaa ctttctcaac
     3301 ccgttaatct gaattgacag gttataggta aagaatttct aggggatgcc agtatcagtt
     3361 agcgcttgac aagaaacggg cttgattttt cgtggctcga ctgcggggtg tagcgcagcc
     3421 cggttagcgc actggttttg ggaaccagga gtcggaggtt cgaatcctct caccccgacc
     3481 aggattttct tgtactggat tcccgcctgc gcgggaatga cattgcacta gagaatcact
     3541 aaacaacaca gttaagacag atcagatgtg ggcgcttagc tcagctggca gagcgcttgc
     3601 tttacacgca agatgtcagg ggttcaagtc ccttagcgcc caccacttat gacttcaggg
     3661 tgaatgatga agatagccgt aaccggtgcc aatggctttt tggggagcaa actcgtctca
     3721 cggttgaccg gttacaagcg gcaaaccgtt gccctgctgc gtcccaacgc ggatagaagt
     3781 ctgctctcgg gggatcagga aatccgagag ctggactact ccaatcccgc ctccatccga
     3841 gccgcgatca gcgattgcga tgtagtcatc cacaatgccg gcaagacccg caccctgcgt
     3901 tttgaagaga tgctggatgc caatctgggc atcacggcaa agctgatcga ggctgtaaac
     3961 ggctcatccg tagagcattt tatctatatc agttcgcaag ccgcttccag accgtccatg
     4021 gatggcgagg ctatcaacga aagctctgag cccgcgccgg tcaactggta tggcaagagc
     4081 aaactctggg ccgaacgagc ggtccaatcc cgctgccaga aagcctggac gatagtgcgt
     4141 cccgttccgg tttacggaga gggtgataga gactttctga gcctgttcag gatggccgcc
     4201 agcggttttt ccttcagcct gggttcccag cggcaaatcc tcaacatgat ccacgcggac
     4261 cagctctgcg actttatcca gacctgcata gccactcccg cggccagaaa cgagctgttt
     4321 ttcgcttcgg acggccaaca ctatacccag ggggagataa tatccatgat tatggacgcg
     4381 gtggggaaga ggcccttact aaaggtggaa atccccatag ccctggccca gcctgtcttt
     4441 cagatgggag aacttctgtc aatcatgagg ggcaaggcga gcctggtggg caggcaaaaa
     4501 tatctggagc tcagcgctcc gcactggaat tgcgacgttt ccaaagcccg gcgcctgttg
     4561 ggctggaatc ccgaactgag tatcgaaaga cgcatgcagg agacctggct gtggtatcgc
     4621 gcccagggct ggctgtaatc ctcctggcgc tcagcgtatg gctgacaggg ttgatggctg
     4681 aaaccgtcag cccgggtccc agcgctccca tgcggcccgt ctatctgagt gaagctcctg
     4741 ctctggagct tagcgcgacg gacgcgagtt tgccgcagag ggttttgatc agaatagcgg
     4801 tgtttccgga ttccagttac accctgctga gcgtggaacc cgcaaacctg agggctgagg
     4861 tggaacgcta tctgcggcag acggacctga tgggatatct ggatgataaa accccagggg
     4921 tcaaagaact ggagatcaga gtcagccgta ttgaacaagc cgcaatggag gctactggtc
     4981 acacaacaga gccagaactg cgccagcgga tcgatgattg gatattcaag cagcgggagt
     5041 tggattctcc tggagcggtc tggagagact ggctggatcc cctggcgtcc gaagatctga
     5101 actatcgaag cgcgaccaac gtaagcggtc tgcacaccgg gaccgaccct ctcaggcagc
     5161 acggcatgga tctggcaaac accccgctgg cgaaagtcca gaggctgaac tggctgaggt
     5221 caatatactc cagcgcggta gcttacgatc attatgacga gccgtatcct tatgaaattc
     5281 tcctgacagg tttgaatgcc ggaatgggtg aatttgacag ccgttatgct aaagtgcgag
     5341 ctggcaggaa tggacttctg ggccgcgagg ggctgtttta cggattggac ctcacagtga
     5401 gcagcggtga ctggttaaac ctcagttccg cgcaaaccaa tatccgccag agtcttagaa
     5461 tccccctggg gaaactgggg ctggagctcg attatgccag tttcgcggcc gaccaggcca
     5521 gcatcagtct gcatccaagc tattggcgtt cgccgcttta cggcctggaa cgcaaaggcg
     5581 aactgctgtg gacgcggctg agctctccct ggctggacct ggcttattcc agaagcagag
     5641 aagctctaaa gtctgatgta tttgccttcc ggcctctgag tcttatgcat cagctccagg
     5701 cggccaaaga actgcaaatg ggcaagataa aaatgggcgc gagttggatt catgcctggc
     5761 gggaaaacaa tctgctgctt cccctggaat catatcagag cgaatataag gacctgggag
     5821 ccgtgaagct tgattatcag ggcgataaac taactgtgaa cgcatccgcc gctctggcag
     5881 acctggcaga cctgatggcg gaaggctcgg tggcatacag gtttggcaag cttacatgct
     5941 cggcaggcct gaaagccgat ttcagcgatt atcagccaag ggtagctgtc acagacattt
     6001 ataccagcaa cgcggagctg gaaagcgtgg acctgagaga gcgtcagcgg ctgtggatga
     6061 agctggactg gcaacacagc gctctaaagt tggccctcac agccggcaga aaagactatc
     6121 aaatcgcctt gccgaacagc ggcaacactc tgcaatataa ggccgctccg ctctttgtgg
     6181 gcctgggggg agtctatgat ctggacctgg gtttcatgcg agcgatcctg aggcagaatc
     6241 tgctctggac ccaggagcag gaacacacgc gagacgatcc agccatccgt ggctttggcg
     6301 agctggaact acgcagggaa ttgagctata acaacgccat cttcgccgct ctgggctata
     6361 ctttccattc cagctatctc aacctggcag ttcagccaga cgtgctggat ttctctttaa
     6421 tcgcggacgc gcgtttcgga gtgaggattt ccaagctgtt tgagatcgat gccggcatcc
     6481 gcaacctggg cgacaactat atctttggag tgtatccgat tccgttttca ctgcacgcgt
     6541 cggtgagatg gtattttgtg aattaggtga gatgnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnntcgtga tactcctgat tattaaacac cagttcttca tgggtgccgt
     6721 ggtgacagat gccggatgag tcgatataga ggaccttatc cacatagcgg agggtggaaa
     6781 gccggtgtga gatcacaatg gcggcgatgc cgggatagtt ggtattgatg tcctgccaga
     6841 gcttttcctc gttttcggcg tccagagatg cggtgatatc atccaggatc agcagttggg
     6901 gatgtttgat cagggcgcgg gcgatggtga cgcgctgttt ttggcctccg gagatgccga
     6961 tgccgcgctg gcctactttg gtttgatcgc catccgggaa ggaggcgatc tcgtcggcca
     7021 gttgagcgct gtttacagcc agtctgtact cattatcgga ggcgttggaa acagcgaatc
     7081 gcacattgtc ctggatcgta ccgctgaaga gcaccggttc ctgaggcacg taggccacga
     7141 tctctctgag ggcggtgata tcaagtgctt cgatggggat gccgttcact tcgatgcgtc
     7201 cggaccgctt ttcaagcagg cccagcatca ggtttacgag ggtgctcttt cccgctccgg
     7261 tagagcccaa aatcaggagc ttctctcctt tggccagctg gaatccgcaa tccgcgatca
     7321 cgggcttgtc tttttcggga tatgcgcaga ctacctggtc gaagcggagg ctgtcaaagc
     7381 tctccacttt gtgggttcca agctgccgtc tggccaggct gggaaatccg cgcatctcct
     7441 ccagacgatc gatattgacg aaggcctgtt tgcctgaaac aaagagctgc gggaggtcca
     7501 ggagcggata gatcatcatg ctgagatagg tgtaaaagag gaagaaagtg cccacgctga
     7561 tctctccttt cacggccagg tagccgccaa agaggatcac gccgatctgg gcgaagtagt
     7621 ctatgtattc ataaatgagg tggaggatgg cgtttagctt caccacgctc atctcggtct
     7681 tgaagcgctt ggccagggcg gtatcaaaaa agcggttgta tttttcctcg ctcacaaagg
     7741 ctttcacgat ccggatgccg gaaaagctca tctccagctg gttgttgatg gcgctgatgg
     7801 cctgctggtt gcgctcgaaa ttcttataga gcttgtctga ggtcacataa aagaccacca
     7861 tcatcagagg cagcggcgcg atcgaaaaga gggtgagttt ggtgctgacg ctgaacatca
     7921 cgatcaggct gaaaaggatc atggagcctg aattgacggc cctgaagata ccggagcaga
     7981 ggaaccagga gatcttggga aagtccgaaa ggtcatccgt gaggcgggtg acgatatcgc
     8041 cggtgcggta tttctggaaa aagatgaagt ccttgtccgt gatagcgttg aaatacttca
     8101 tccgcaagag atgctcgaag cggatattca cccaaccccg gaaggcggga taaaatcctg
     8161 tgatcaactg cgccaggccg atggccataa acatccagat cacgcggttc acggccccca
     8221 tcggttcagg atatttctgg ggactggtga ggatatcgcg caggaggtcg atgagccttc
     8281 taaacacgat ggggtaggcg atggaaaccg ctgaggaccc cagcgtgaaa aacagcatga
     8341 tgatcaggaa ccagcgctgg gtgtaccact gcctggcaat ccaggccaga tggggattca
     8401 tcctcttcag gaggccgcgg gcgaagcgtt tactcattaa cggcctcccc tctcaacatc
     8461 tgtagctcca ccagcttacg atattcggga gacttgtgca tcagctcgtc atgagtgccg
     8521 cggtggatga tctccccctg gctgaaaaag agaatctcgt cggcatccat taccgaggtg
     8581 agccgatggg ccacgatcac ggccgtgcgg ctctcaaaca cgctgtccat actgcgctgg
     8641 atgcgtgctt ccgtctgagg atctatggac gcggtagctt cgtccatcac gatcaactcg
     8701 gcatcgaaag ccagggccct ggcaaaactg atcagctgct tttcgccctg agagacgttt
     8761 tggccccgtt ccgccagctc ggtattgagc ccatcagtct gggctttgat caaatcgtcc
     8821 agttgcacga tgttgatggc gtccctgact ttggattctg gaacgaggtc gttatagacc
     8881 cgcacgtttt ccaggatgga gccagggaat agaaagatgt cctgcaggat gaggccgatc
     8941 ttggccctcc aggccctgaa atccaggtct gacagcgcta ctccgtcgat ccggatagca
     9001 cctttctgga tagggtaaaa gccgcataaa aggctcacag tggtggtctt gccactgccg
     9061 gaagctccta ccagggctac tttcttgcct ttggggatag tgaaactgac gtccttgagg
     9121 atccaatctt cgtctttgta ggcaaaccac acgtggtcaa agcggatgtc atgctcaaac
     9181 acgggagttt gggtgccggt gaagagctct tcttcggtgc ccagttcagt cagatcgaga
     9241 atgcgccgca acgatacaaa ggagcgttga atctgcatga cgttttcgga gatctgcatc
     9301 agcggccaga tcatgcggtt gagatactgc acaaagatga tcaaggtgcc cagagtgacc
     9361 gctccggcta tgatcttggg ggccgtgagg ctgatcacca gaatgatgaa agccacttga
     9421 aagataaaag taaagaggct ctgagagccg tactccgtga aggaagtgcg ggtctccagc
     9481 ttgtatttgt cgtaggaggc catccggagg tcttccacca cgcgcttttg ttggttgaga
     9541 gcctggatca tcggcatccc ctgcacgtaa tcagtgatgc ggccggtgat atcggcatac
     9601 ttttcccgga taagcctata gaacttcgag agatagcgga tcaggtaata gtagcccacg
     9661 atcgcagtcc ccatggcggg caggatccaa attgtgacct gccactgcct gataaacagg
     9721 accacaaaga ctccgataaa aaagaggaag ttgccgataa tgcggatgga aagctctgaa
     9781 aacagtacct ttacacgctc gctgtcactc tccacccggg cgatcagttc tcccacggac
     9841 tggcgattga accattccac cggcagaatg agcaagtgcc tgaacacacg gcctttgaag
     9901 ttggtgatca ctttgatgcc caggcgggac agcagcacga tctgcagata ggaaagcagc
     9961 ccggaaacca gcaccacgcc tacaaatatc atcccatatc tgaaggtctg ggtcatatct
    10021 ccagccggaa cgctcttgtc cactatcaag gccaggatga gcggatctac cagttgcagg
    10081 gaactggtga tcagcatggc catcaatcca aagaacagca atcccttgtc ctgcttcaca
    10141 taaggataga ggagcctgaa gtagtaggat aggttctgtt tttgttcttt catggtggtc
    10201 catacgcttt tgagagttga tttatgagcg gaagccgagg ccgcgaaata gatcgcgccc
    10261 ggcttcggca agccgggcgg atgaatagta agaccgaaaa ctggtcggga tgagaggatt
    10321 tgaacctccg acttctcggt cccgaaccga gc
//
LOCUS       DAOSFN010000085        10271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5541_length_10271_cov_10.438038, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000085 DAOSFN010000000
VERSION     DAOSFN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5541_length_10271_cov_10.438038"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..659)
                     /locus_tag="PLO35_06465"
     CDS             complement(204..659)
                     /locus_tag="PLO35_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79345.1"
                     /translation="MKQLLVTFVLLTAAIGLCAQTGLFGISFGQSRADVQSNLTKAGF
                     TVEESKDTQIRYTNPKIENLTELAVEFSFEGGVKSWEIYYSTIDKPGVLDDVMKQLSD
                     LHGSEPWWDDYFEEWIWDLENDMAVYMYEGEDYIRVDYDVWDDSFDYWW"
     gene            complement(734..1372)
                     /locus_tag="PLO35_06470"
     CDS             complement(734..1372)
                     /locus_tag="PLO35_06470"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH79346.1"
                     /translation="MRKFIIMLFLLGLVTLWADENAADPVKAYLESPDISGFQAAVAQ
                     LSTQLGNEDSDFRARLYLHHLCDLEATRMLDDLIADADSLAPGERFSLANYLLGKEEY
                     QAAVKLYDAINEDLPNWSCPWRHKGEALYKLKDYQRAMTALEQAIATNQNHYDAYIWM
                     ARAQKELGLYPDALANLDRARKLNPGEEGHEDEEIPAEDIDKLYKELQELLR"
     gene            1522..1968
                     /locus_tag="PLO35_06475"
     CDS             1522..1968
                     /locus_tag="PLO35_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH79347.1"
                     /translation="MIEVLQLFKLDDIDRHLGRETVVSFLSTHLERWRDDEFSIEKAI
                     DYVLDPLPGKGGFILAAIEAGELAGILVMLKTGMSEFVPQNLLVYIAVDSAKRGRGIG
                     KLLINKAKDISDGDIALHVEPDNPARHLYSHLGFGSKYIEMRWKRN"
     gene            1971..3047
                     /locus_tag="PLO35_06480"
     CDS             1971..3047
                     /locus_tag="PLO35_06480"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOH79348.1"
                     /translation="MARLKINLDALTANIESITELMTVNRKSWSLVVKALGTDKRVLS
                     VLLSHPCVKAVHSVAVSQWKALRLVKEIDPSLRTMYIKPPVLKNVPQIVKYADISLNS
                     SFATIQALSNEAVKQAKTHEIIVMIEMGELREGILREGLEAFYKRIFRLPGIKVIGLG
                     TNLGCMYGVRPTYDKLIQMVLNKQLVELKFKRKLELVSGASSITIPILQKGKVPEGIN
                     HFRIGEAVLLGTSPLNGKKVHGLSIDAFSFEANIVELYKKETVPDDIDPQTSVTGQGL
                     SPTPDNTSFRALLDFGILDVDPDQLVPVSPGIRFAGISSDLSVYDLGDNAQRLRTGDV
                     IRFKPKYLGVARAMYSGFVEKVIE"
     gene            complement(3392..4741)
                     /locus_tag="PLO35_06485"
     CDS             complement(3392..4741)
                     /locus_tag="PLO35_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOH79349.1"
                     /translation="MMGIKVLVADDDSIFCRLVKDILQKDDYQVTVALDVEEARRETS
                     NQSFDIIMLDMCFPSLRDGFQLLDEFHSQFQQSVILMISGSGHIPDAVLAVKNGAADF
                     IEKPIEPRELSLRVQALADRINRTRASLVHQYPSIGMVGTSKQMISVFESIVKASSYD
                     TPVLITGETGVGKELAAHAIHRLSKYATKPMVSINCASVPKDLFEAELFGYEQGAFTG
                     ATKSREGYFEFARDNSIFLDEIIELPKTLQAKLLRVISEGEIQKVGGKVVPIDTRIIS
                     ATNQDIEAAVVKGLFREDLLYRLNTLHLRIPPLRERVEDIAPLARNFVKDFCARNDLP
                     VKQITPNAMAWLQHQPWNGNGRELRNSVERALIFARGDELDLADFTSQADLLKAASAV
                     GADNKLRDALRLFERNYIEYHYRANNYNLTRTAEAMGIDKSNLSKKISALSINPHDL"
     gene            complement(4728..7196)
                     /locus_tag="PLO35_06490"
     CDS             complement(4728..7196)
                     /locus_tag="PLO35_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79350.1"
                     /translation="MRSIAVSVMALMAIMLAGCRPPGHFHTHVLNPLYKHVLDSPATN
                     WIFADLQGNEEEYFIATDNQFGNINSLYVFHPNGKTISQLAMSQKFRGLRALTDPRNG
                     NRWLFYSFNDQRRVTVTGLKYTWEVPLKREEKLFESIARTDTRIDDPSLEWFALVYPE
                     ILEDIDGDGKLEMVCRMLDGYMANPRGLVVYDFDSGKIKWRFDTSANFSSVLLADHDR
                     DGKKEFILSSQAVMNTSAAINGFDDQSGRVAVLDTSGKILYQEKLFDGYGDIQLTSID
                     PDQDGRPEIYAIISTWGARNIRNSVIQLKWENGHLRKIKTWALGTYFDRGAQPYQFQD
                     LDGSGTWRLPLVDKGSGLVVLDENLKPVKHGYKDFIKQIWAVEDINQDGVKEILLLTA
                     DNNFVVLDHHFRELASLKSPFPGDERTAMQAVKTGFSKPRTIALWSKSQLQYFELKVE
                     PFYKLAFRLLAEYLWVLTLLLAMAMLYACYIIYYNWTMFACFGKEVGLGVVNSTRKGR
                     VRRMNAVAMSVLSPDPDKLTERSLARLHPELARLVKSLVKSRENNCETTLELEDESKQ
                     KSFRVLAYKQTGLNGGVMLILFPRTYDLDADKAVWADTARRLSHHVRRHITNVILALP
                     RLEDENDGEDKKEFLDIIRDEIEKIRVFTHSFQRFTELKDYDLKAQDIIPSLEHVVAR
                     ITLPEKITLIKNWKLNSVHAFIEPIRFEEALTNLINNAVEAMPEGGILHVSAKVFPHH
                     TSPKGPLSVLVEIEDSGVGIPAKYMDEIWKPFFTTNQSGTGIGLPESCKIITSMGGEI
                     SVQSEEGVGTVVSVWLKGEGDDGH"
     gene            complement(7486..8790)
                     /locus_tag="PLO35_06495"
     CDS             complement(7486..8790)
                     /locus_tag="PLO35_06495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH79351.1"
                     /translation="MPKRVLYLSYFYPPLGGPAVLRNLKTVKYLSRAGFEIDVITVAD
                     LEYLYRDETLTSQQSERRLFRTRSADPMALLKKLRPASSDEASRIYMNTPEKAKLMIR
                     RLWPIDNKIGWLPFLLQTGRKALRENEYDLIYISLGPFSSGLGAYWLSKESGLPLVLD
                     MRDYWTLLGDYELQGFGWQRRLSRHWEARLYRHASLIVTATQGIGEDICEAFGAGLQS
                     KCLTVYNGWDEEDFEGLDAIHPGQGFDLAYFGNIYARRSLGKFYAAVSSLRGEGILPT
                     NTRIKLYGNFFRETVEEAQSSGVSDLVEFVPQLGHREAIRAMLSSHVLLLTLNSSGPR
                     GTLSSKIFEYLRAGRPILAMVPAHKEAASLLRDCGQNYICAMESESSIRQALMRLIAE
                     YEAEKRYPAPPELERAAQISKLAAALESIKNKSSIQGRHSSK"
     gene            complement(8790..9923)
                     /locus_tag="PLO35_06500"
     CDS             complement(8790..9923)
                     /locus_tag="PLO35_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOH79352.1"
                     /translation="MKICIVTNSHHSTDVRLYYKIALSLAKLGQVYLLCASGVRQHSV
                     NPYQIVVDSDSPWAALRQLYNQACHLRPDLVICVEPLTMLVGMRIRRHLGCKVIFDIH
                     EFFADAFAERFSPPFSWLMKLVYLMGERWLSSRADACIGVSDKILDQAVPKSKRKLAI
                     AIPNYPVKHVWDYNCETPLELSLLCETDFDLIYIGGLTRDRGILRLLQAVSLLRKDIP
                     RLNVLILGKFHDPGVEKEFNDTVNDLNLTGVIYYQSWIPAEKIGLLLKRSRVGLWTFN
                     PASRRMKRAVPLKVLEYFAAGLPVVSTKSPLMRDLVETNRIGELCEYNSQSIAAAALK
                     ILKLPHDEYEKMQARAIDLIESRFNWEAVEPRLLDLASRLAKR"
     gene            complement(9974..>10271)
                     /locus_tag="PLO35_06505"
     CDS             complement(9974..>10271)
                     /locus_tag="PLO35_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79353.1"
                     /translation="HSLPLKHLCPSVPLFLENSLLFSVFDLCFSLCLNVMRDALQSRA
                     DWEIDATAPGNDDVSGSTREGILNSHISVSPKPLSLCPSVFKRIISSETLPVQI"
BASE COUNT         2323 a         2681 c         2789 g         2478 t
ORIGIN      
        1 ccgcaaactc gttgtcatgt atgcctttat cgtgaccgct tgaactccaa cgggtcaata
       61 tcagtttttt gacttcgcca ttttcgcaca agctcttcag ccagtgttac aggagcagac
      121 gcgcgttggt cggtatggaa accaaccctt cggacagact cacgcgcacg ctaccctaaa
      181 accctatgcc tctgaatccc gtcctaccac caatagtcga agctgtcatc ccacacatcg
      241 tagtcgacgc gtatataatc ttcgccctca tacatatata ccgccatgtc gttttccagg
      301 tcccatatcc attcttcaaa ataatcatcc caccatggtt cgcttccgtg caggtcgctg
      361 agctgtttca tgacatcatc caagactcca ggcttgtcta tggtgctata gtagatttcc
      421 cagcttttca ctccgccctc gaagctaaat tctaccgcaa gttcggtgag gttttctatc
      481 ttgggattgg tatagcggat ttgggtgtcc ttactctctt ccactgtgaa gccagccttg
      541 gtgagattgc tttgaacgtc agcacggctc tggccaaaac tgatgccaaa gagaccggtc
      601 tgagcgcaga ggccaattgc cgctgtcagt aatacgaaag ttacaagtag ttgtttcatg
      661 tttcacctct tatacagcta taatagccgt tctcaatagt tctgcaagga caattatagt
      721 ctgggaggcc agcttagcgg agcagctcct gtaattcctt atagagctta tcgatatcct
      781 cagcggggat ttcttcgtcc tcgtgccctt cttctccggg attcagcttc ctggccctgt
      841 cgagattggc cagggcatcc gggtagaggc ccagttcttt ctgggccctc gccatccaga
      901 tataggcgtc gtaatgattc tgattggtgg cgatggcctg ttccagggca gtcatggcac
      961 gctgataatc cttcaatttg tagagggctt cacctttgtg gcgccagggg cagctccagt
     1021 tgggcaaatc ctcatttatg gcatcgtaga gcttcaccgc ggcctggtat tcctcttttc
     1081 ccagcagata gttggccagg ctgaaacgtt cgcccggggc cagagaatcg gcatccgcga
     1141 tcagatcgtc caacattctt gtggcctcca gatcgcagag atgatggagg taaagccggg
     1201 cccggaagtc gctgtcttca tttccgagct gagtgctgag ctgcgcgacg gcggcctgaa
     1261 aaccagagat gtccggagat tccagatagg ctttgacggg gtcggctgcg ttttcgtcgg
     1321 cccagagggt tacaagaccc agcaggaata gcatgatgat aaacttacgc atagatggat
     1381 tccttttttt gtagtcaatc tgaatagagg tcaggctggc agggcagagg gaatggtcaa
     1441 gaaaaaaaag acttgacagt ggttaaacgc aactttaaca aggtattgtg ctaataagaa
     1501 ttgagaagaa ggagtgataa tatgatcgaa gtattgcagt tattcaagct ggatgatata
     1561 gaccgacacc ttggcaggga gacagtcgtg agtttcctgt ccactcattt ggaacgctgg
     1621 cgcgatgacg agttttccat cgaaaaggcc atcgactatg tcctggaccc cttgcccggc
     1681 aaaggtggtt tcattctggc agccatcgaa gcgggagaac tggctggaat tctggtaatg
     1741 ttgaagaccg ggatgagcga gtttgtgccc caaaacctgc tggtctatat tgccgtcgat
     1801 agtgccaaac gaggacgggg aatcggtaaa ctcctgatca acaaggccaa agatatcagc
     1861 gatggggata tcgctctgca tgtggaacca gacaatcccg cccgccatct ctactctcat
     1921 ctgggctttg gcagcaagta tattgaaatg cgctggaaaa ggaactgagc gtggcccgcc
     1981 taaagatcaa tctcgacgct ctaaccgcca acatcgaatc cattacagaa ctgatgaccg
     2041 tcaaccgcaa atcctggtcg ctggtggtaa aggctctggg cactgataaa agagtgttga
     2101 gcgtactgct aagtcatccc tgcgtaaagg ctgtgcactc cgtggctgta tcccagtgga
     2161 aagccctgag actggtgaaa gagatcgatc cctcgctgcg caccatgtat atcaagcctc
     2221 ccgtgctgaa aaacgtcccc cagattgtga aatacgcgga tatatctctg aacagctcct
     2281 tcgccacgat ccaggcactc agcaatgaag ctgtcaaaca agccaagacc catgagatca
     2341 tcgtgatgat agaaatggga gagctgagag aggggatcct gcgagagggg ttggaggctt
     2401 tctacaagcg cattttccgt ctgcctggca tcaaagtgat cggcttgggg accaacctgg
     2461 gctgcatgta tggggtgcgc cccacctacg acaagctgat tcagatggtg ctcaataaac
     2521 agcttgttga gcttaagttc aagcggaagc tggagttggt ctccggggcc agcagtatca
     2581 ccattcccat cctgcaaaaa ggaaaagtcc ctgaggggat caaccacttt cggattggag
     2641 aagccgtatt gctgggaact tctcctctaa acgggaaaaa ggtgcatggc ctctccatcg
     2701 atgctttcag ctttgaggcc aacatcgtgg aactctataa aaaagagaca gtcccggatg
     2761 atattgatcc ccagacaagc gtgaccggcc aaggtctatc ccccactccc gacaatacca
     2821 gtttcagggc tctgctggat ttcgggattt tggacgtaga ccccgatcag cttgttcctg
     2881 tcagtcccgg aattcgtttt gcggggatta gcagtgatct gagcgtgtat gatctggggg
     2941 ataatgctca acgattgcgc accggcgacg tgattcgttt caaacccaaa tatctgggtg
     3001 tggccagagc gatgtattcc ggttttgtgg aaaaggtgat agaataagca aaaaccggca
     3061 cccgggagac tgggtaccgg ctattagaat ccgctaggcg ggttgacgag tatggccatc
     3121 aggcctgtga ccaaagagcc acttagccta caggcccaca cgtgatatcc tcccttgaca
     3181 cgtagcaatc ctgtaattgc acatataggg agcgattcct gttccgaaag tgataatctc
     3241 agctaacggc atataatgca tataattgtg tcattgttac tgtatcatgg agttgtataa
     3301 tatacctcag gaaggcctga aacgcaaaaa tgggtgtggt atattttacc acaccgatcg
     3361 ggaggtaata tttacataga ccgcttgcgg gttagagatc gtgaggattg atgctaagcg
     3421 cgctgatctt cttgctaagg ttggatttat ctatgcccat ggcctccgcg gtgcgggtca
     3481 ggttgtagtt gttggcccgg tagtgatatt cgatataatt gcgttcaaac aaccgtagcg
     3541 cgtcccgcag tttgttgtcc gctcccaccg cgctggcggc tttgagcaga tcagcctggc
     3601 tggtaaaatc ggccagatcg agctcatccc cgcgggcaaa gatcagagct ctttccacgc
     3661 tgttgcgcag ttcccggccg tttccattcc agggctggtg ttgcagccag gccatggcat
     3721 tgggggtgat ctgtttgact ggcagatcgt tgcgggcgca gaagtccttc acaaagttgc
     3781 gggccagggg agcgatgtct tcgactcttt ctctgagggg agggatgcga aggtggagag
     3841 tgtttagccg gtagagcagg tcttcgcgaa agaggccctt gaccaccgcc gcttcgatat
     3901 cctgattggt ggcactgata atgcgggtgt cgatcggcac cactttgccg cccaccttct
     3961 ggatctctcc ttccgagatc actctcaaca gcttggcctg cagggttttg ggcaactcga
     4021 tgatctcatc cagaaagatg ctgttatccc tggcaaactc aaaatagcct tcccgggatt
     4081 tggtggcccc agtgaacgca ccttgttcat agccaaacaa ttccgcttca aagagatcct
     4141 tgggtaccga ggcgcagttg atgctcacca tcggtttggt ggcgtatttg ctgaggcgat
     4201 ggatagcgtg ggcggccagt tctttgccca cccccgtttc tcctgtgatc aggactggag
     4261 tgtcgtagct ggaagccttg acgatgcttt caaacacgct gatcatctgt ttggaagtgc
     4321 ccaccatccc gatgctggga tattgatgga cgagactggc gcgggtgcgg ttgatcctgt
     4381 ccgccagggc ctgcaccctc aggctgagtt ccctgggttc gatgggcttt tcgataaagt
     4441 ccgccgcgcc atttttgacg gccagcacgg cgtccgggat gtgtcccgat cctgagatca
     4501 tcaatatcac gctttgctga aactgggagt gaaactcatc caatagctga aagccgtccc
     4561 tgagggaggg gaaacacata tccagcatga tgatgtcgaa gctctgattg gatgtctccc
     4621 gtctggcttc ttccacgtcc agggccaccg tcacctgata atcatccttt tgcaggatgt
     4681 ctttcaccag ccggcaaaag atggagtcgt catccgccac caatacttta atgcccatca
     4741 tcgccttctc cttttagcca gacggacacc acggtgccca ccccttcttc gctttggacg
     4801 ctgatctcgc cacccatcga cgtaatgatc ttacagcttt ccggcaatcc gatcccggtg
     4861 ccagattgat tggtggtaaa gaagggcttc cagatttcgt ccatatactt ggcggggatg
     4921 cccactccgc tgtcttcgat ctccaccagc accgaaagcg gacctttggg gctggtgtga
     4981 tggggaaaca ctttggccga cacatgcaga attccgcctt ccggcattgc ttccacggcg
     5041 ttgttgatca gattggtgag cgcttcctca aaacggatgg gctctatgaa ggcatgcacg
     5101 gagttgagct tccagttttt gatcagggta atcttttcag gcagagtgat cctggccacc
     5161 acatgctcca gggaggggat gatatcctgg gctttgaggt catagtcctt cagttctgta
     5221 aagcgctgaa agctgtgggt gaagacgcgg attttctcga tctcgtcgcg gataatatcc
     5281 agaaactcct ttttgtcctc gccgtcattt tcatcttcca gacgcggcag ggccagaatg
     5341 acattggtga tatgcctccg cacgtgatgg gaaagacgcc tggccgtgtc tgcccagaca
     5401 gctttgtctg cgtccagatc ataggtcctg ggaaacaaaa tcagcattac gcccccgttc
     5461 aggcctgtct gcttgtaggc cagcacccgg aagctctttt gtttggattc gtcttcaagc
     5521 tccagagtgg tctcacagtt gttctctctg gatttgacaa gagattttac cagccttgcc
     5581 agctcaggat gcaagcgggc cagagacctc tctgtcagct tgtctggatc ggggctcagg
     5641 acactcatgg ccacggcatt cattctgcgg acgcggcctt tcctggtgga gttaactact
     5701 cccaggccga cttcctttcc aaagcaggca aacatcgtcc agttgtagta tatgatgtag
     5761 cacgcgtaga gcattgccat ggccagcaac aaggtgagca cccacagata ttccgcaagc
     5821 aagcggaagg ccagtttgta gaagggctcc accttcagct cgaagtactg cagctgggat
     5881 ttggaccaga gagcgatcgt cctgggcttg gaaaagccag tcttcacagc ctgcatggca
     5941 gtccgctcat cacctgggaa gggactcttg aggctggcca gttcgcggaa atgatggtcc
     6001 agcaccacga agttgttgtc tgccgtgagc agcaagatct ccttgactcc gtcctgattg
     6061 atgtcttcca ccgcccagat ttgcttgata aagtctttgt agccatgctt taccggcttc
     6121 aggttttcgt ccagcaccac cagaccgctg cccttgtcca ccaggggaag tctccaggtg
     6181 ccggaaccat ccaggtcctg gaactgatat ggctgagcgc cccggtcaaa gtaagtgccc
     6241 agggcccatg tctttatctt gcgaagatgg ccattttccc acttgagttg aatgacggaa
     6301 ttgcggatat tgcgagcacc ccaggtggag ataatagcat agatttcagg gcggccatcc
     6361 tgatcgggat cgatgctggt gagctggata tcgccatagc catcaaatag tttctcctga
     6421 tacaggatct tgccggaagt gtccagcacc gccacccggc cactctgatc atcgaagccg
     6481 tttatagcgg ccgaggtgtt cataaccgcc tgggaagaaa ggataaactc cttcttgccg
     6541 tccctgtcgt gatccgccag cagcacgctg gagaaattgg cactggtgtc gaagcgccac
     6601 ttgatcttgc cgctgtcaaa gtcatagacc accagtccgc gtggattggc catatagccg
     6661 tccagcatcc tgcagaccat ttccagcttg ccgtcgccgt cgatatcttc caggatttcg
     6721 gggtagacga gggcaaacca ttccaggctg ggatcatcga tcctggtatc cgtcctggcg
     6781 atcgattcaa agagcttttc ttcccgtttg agcggcactt cccaagtgta tttcaggccc
     6841 gtgaccgtca cccggcgctg atcgttgaag ctgtaaaaca gccagcgatt gccgtttcgg
     6901 gggtccgtta acgcgcgtaa acctctgaat ttctggctca tggccagctg ggaaatggtc
     6961 ttgccattgg gatggaagac atagagggag ttgatgttgc caaactggtt gtccgtggcg
     7021 atgaagtatt cttcttcgtt accctgcaga tcggcaaaaa tccaattggt ggcggggctg
     7081 tccaacacgt gtttgtatag cggattcagg acgtgggtgt ggaagtggcc ggggggacga
     7141 cacccagcca gcatgatcgc cataagcgcc ataactgata cggcaatgct acgcaaaaat
     7201 gactcccttc aacgtttatt tccaaagaac tgatctttga tttgctgtca atcaaaaaca
     7261 cgggagccgt tcaggaaggc ttgtgtcgct cttcgctctt attcatgtgg ctgaatcttg
     7321 cccatagctc taccggggca aaaccaactt agttggggtt gggtccggct tgcctgacct
     7381 gatatcgcga tcaagggata agggaggcct ggcagggaaa catcaatgcg ctgaagggca
     7441 tgggattaga tcatgtcgcg cggacagacg agaggggtag gcgaattact tgcttgaatg
     7501 ccgtccctga atagaagatt tgttcttgat actttccaat gccgcggcca gtttgctgat
     7561 ctgggccgct ctttccagct ccggtggggc tggatagcgc ttctccgctt catattcagc
     7621 gatcagacgc attaaagcct ggcggatgga gctctccgat tccatggcgc agatatagtt
     7681 ctggccacaa tccctgagca gcgaggccgc ttccttatga gccggcacca tggccaggat
     7741 gggccttccg gccctgagat attcaaagat cttggagctc agagtgccac ggggaccgct
     7801 gctgttgagc gtgaggagca ggacgtgcga gctcagcatg gcccgaatgg cttcccggtg
     7861 accgagctgg ggaacaaact ccaccagatc gctcactccg ctgctttggg cctcctccac
     7921 agtctcccgg aagaaattgc cgtaaagctt tatccgggta ttggttggca gaataccttc
     7981 gcctctgagg gaacttactg cggcgtaaaa cttgccgagg ctgcgccgcg cgtagatatt
     8041 gccaaagtaa gccagatcaa agccctgacc gggatggatg gcatccaggc cctcgaaatc
     8101 ctcttcatcc cagccgttat agacagtcag acacttgctt tgcagcccag cgccaaaggc
     8161 ctcgcagata tcctctccaa tgccttgggt ggctgtaact atcagactgg catgacgata
     8221 tagccttgct tcccagtgcc ttgaaaggcg tctttgccat ccaaaaccct gcagctcgta
     8281 atctcccagc agggtccagt aatcccgcat gtccagcacc aaaggcaagc cgctctcttt
     8341 ggacagccag taggctccca gtcctgatga gaagggcccg agggagatgt agatcaggtc
     8401 atactcattc tcccgcaatg ccttgcgtcc cgtctgaagc aggaaaggca gccagccgat
     8461 cttgttgtcg atgggccaca gcctcctgat catcagtttg gccttttcag gagtattcat
     8521 atagatgcgg gaagcctcat cgctgctggc gggacgcagt ttcttgagca gtgccatggg
     8581 atctgccgag cgcgtcctga agagccgtct ttcgctctgc tggctggtga gcgtctcatc
     8641 ccggtagagg tattccagat cggccacggt gatcacgtcg atctcaaatc ctgcccggct
     8701 tagatacttg actgtcttga ggttgcgcaa cacggccggt cctcccaaag gaggatagaa
     8761 gtagctgaga tagaggacgc gtttgggcat tagcgctttg ccagccggct ggccaagtcc
     8821 aggagccgtg gttccaccgc ctcccagttg aagcgacttt cgatcaggtc gatagcgcgg
     8881 gcttgcatct tttcatattc gtcatggggt agtttgagga ttttaagcgc tgccgcggca
     8941 atggattgag agttgtattc acagagctcg ccgatgcggt tggtctccac caggtcccgc
     9001 atcagaggag acttggtact cacgaccggc aggcccgccg caaagtattc cagcaccttc
     9061 agaggaacgg cgcgtttcat tctgcggctg gcgggattga aggtccaaag ccccacccgg
     9121 ctgcgcttta acagcagtcc gatcttctcg gcgggaatcc agctctgata atagatcacg
     9181 ccggtgaggt tgagatcgtt gaccgtatcg ttaaactcct tctccactcc gggatcgtga
     9241 aacttgccga ggatcaatac gttgagccgc ggaatgtcct ttcggagcaa ggatacggcc
     9301 tggaggaggc gcaaaattcc ccggtctctg gtgaggccgc cgatataaat gaggtcaaaa
     9361 tccgtctcgc agagcaggct gagctccaga ggcgtctcgc agttgtagtc ccagacgtgt
     9421 ttcacagggt agtttggaat ggcgatggcc agtttacgtt tggatttggg cactgcctgg
     9481 tccaaaatct tgtcgctcac gccaatacag gcatccgctc tgctggaaag ccagcgttcg
     9541 cccattagat acacgagctt catcagccat gaaaagggag gactgaagcg ctcggcaaag
     9601 gcgtcggcga agaactcgtg gatgtcgaag atgaccttgc agcccagatg cctcctgatc
     9661 cgcattccca ccagcatcgt gagcggctcc acgcagatca ccaggtccgg cctcagatgg
     9721 caagcttgat tgtaaagctg cctgagcgcc gcccagggag aatcggaatc caccacgatc
     9781 tgatagggat tgacgctgtg ctgcctaact ccgctggcgc aaagcagata cacctgtccc
     9841 agcttggcca gactgagggc gatcttgtaa tacagccgca cgtccgtgct gtggtgggag
     9901 tttgtaacga tgcatatctt catggcttta ccttcgtctt tactcaaaca aagaggcctg
     9961 tattgctgtc aagctaaatc tggactggga gggtttctga gctgatgatt cttttgaaaa
    10021 cagagggaca aagagacaga ggttttggag agacagagat atgagaatta agaatgccct
    10081 cccttgtgga gccggaaacg tcgtcgtttc cgggggcggt agcgtcgatt tcccaatcgg
    10141 cgcgtgattg taacgcatca cgcatcacgt ttaaacacag agaaaagcag agatcaaaaa
    10201 cagagaaaag cagagaattt tctaaaaaca gagggacgga gggacagaga tgtttgaggg
    10261 gcagagaatg g
//
LOCUS       DAOSFN010000086        10200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5595_length_10200_cov_10.069492, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000086 DAOSFN010000000
VERSION     DAOSFN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5595_length_10200_cov_10.069492"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..1052)
                     /locus_tag="PLO35_06510"
     CDS             complement(312..1052)
                     /locus_tag="PLO35_06510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH79354.1"
                     /translation="MEINDAIWQDYDSSGQIDAKLATIRKWIPEDAGTIIDVGCGNGI
                     ICNALASEYEVTAIDISETALKAVKVSKLLASATDIPFADNSFDLAFSSEMLEHLSTP
                     DLEKAAREMVRVARKYLLISVPNREQLGKSMVKCKVCAEVYHAYGHLHSFTDSSLRGL
                     FPGTQKLDSLCFGPTSKDYHPLLLRVRNHLGGQYFHPAAPVLCPRCGSDTFVYRSNPV
                     SKACNLLNNAISRPKPYWLMILLVKVGQ"
     gene            complement(1744..3021)
                     /gene="eno"
                     /locus_tag="PLO35_06515"
     CDS             complement(1744..3021)
                     /gene="eno"
                     /locus_tag="PLO35_06515"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOH79355.1"
                     /translation="MSEIMYIKGREILDSRGNPTVEADVHLESGVVARAAVPSGASTG
                     EREALELRDGDKSRYQGKGTLKAVANIDDEIAPQLCGNESSQQYAIDQLMLKLDGTAN
                     KDKLGANAILAVSMAVARASAIELDIPLYRYLGGVNAVTLPVPMSNIINGGAHADNNV
                     DIQEFMIMPLGAPNFREAIRMNAETFHALKSILKKRGLVTSVGDEGGFAPNLGSNEEA
                     LQVIVEAISAAGYKPGTDIFIALDAAASSFWKDGGYLFEGQQRDSAWMIDYYERMIGK
                     YPIVSIEDGLAENDWDGWKMMNERLGKKIQIVGDDVFVTNPAIIKRGIKEDIANSVLI
                     KLNQIGSVSETIDAINTAHKAGWTCVVSHRSGETGDTFLADLAVAMNTGQIKTGSLSR
                     SERVDKYNQLLRIEEELGSAAIFPGKAVIKQLG"
     gene            complement(3118..4098)
                     /locus_tag="PLO35_06520"
     CDS             complement(3118..4098)
                     /locus_tag="PLO35_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOH79356.1"
                     /translation="MLPVSLLLVSQSVATGLWVMIAIAIFVVVIVAKGIIVVRQAEVV
                     IIERLGKYYTTLQSGIHIIVPVFDKTRPIHWRYNKTDYRGQTIQVQRVENRIDLRETV
                     YDFPRQNVITSDNVSININALLYFQVTDPYKSVYEIGNLPEAIEKLTQTSLRNVIGEL
                     TLDHTLTSRDAINAKLRDILDDATDKWGVKVNRVELQEILPPEEIRTAMEKEMRAERD
                     KRAKMLTGDADREFQIRVAEGDKQAKIARAEGEAQAKLLVADAERKSIQMIAEAVKDS
                     KTDPAQYLIALKYVAAFQEITKQGDKTVVIPYESAALIGSVKTLANIFEK"
     gene            4311..4871
                     /locus_tag="PLO35_06525"
     CDS             4311..4871
                     /locus_tag="PLO35_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79357.1"
                     /translation="MRITILSVLSLMLILSGCAFRKRERPDPDSGIGLSGSFSAKDSP
                     LVAREISLEITSHPWLQQFHGSRQRSPVIMIGILTNESYQTIDMDLFGRQLMHEILNA
                     GELRFVEELPVPLRERSEPSLDLARELAADFYLTAIVRTAEQQYKSKRIRYLQLDMKL
                     LEVATGQPVWTILRPIRQNPTQGAAQ"
     gene            4927..5829
                     /locus_tag="PLO35_06530"
     CDS             4927..5829
                     /locus_tag="PLO35_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOH79358.1"
                     /translation="MQITKLEQMLEVLKSRHKKRLVAAWAIDAHTIVAVSEAVGMGII
                     DAILVGDEAIIKDVCAQHGIDPSIFRIVSAISDVDGAAKAVELINAGEGDFLMKGLIS
                     TDRYMKAILNKEKGLMENGAILAHVTVAEPMGYHKLLVFGDVAIIPEPDLNQKIAICN
                     YLIKTAKLLGIDTPKLGILSASEQTLPKIRSSYEAALISKMAERGQIKGAIVDGPLGF
                     DLIIDKQSAEIKGVKSEVCGDADCILFPNIEAGNTFYKTITCLMGSELCAIVMGAKVP
                     AVLTSRGDSERSKLYSIALAAILA"
     gene            5831..6748
                     /locus_tag="PLO35_06535"
     CDS             5831..6748
                     /locus_tag="PLO35_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOH79359.1"
                     /translation="MKAITKLSELASHVRDRGFKARVAVAAAEDQNTLSSIARAVSEG
                     WAHAILHGRQARIEEVCRAEGLEPSLFEIIDHPDELSATLEATRQVREGEADVLMKGL
                     VGTDKFLKAVLDKQKGLLPPGAVMSYTCALELPKYPKLLFVSDTAVLPDPDLAQTQAM
                     INYSVAMARRFGIECPKVALISATEKVGPSMPSTLEHALLCKMNQRGQIKNCLIDGPL
                     DIFLACDPAAGEIKGVKTPINGEADVLIFPSLESANSFYKGLMLFAGAELAGLIQGTV
                     KPVVVMSRSESAASKYYCVALACLMAEEQ"
     gene            6745..7185
                     /gene="rpiB"
                     /locus_tag="PLO35_06540"
     CDS             6745..7185
                     /gene="rpiB"
                     /locus_tag="PLO35_06540"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOH79360.1"
                     /translation="MKLAIASDHAGYALKEALKFAFPEHEWVDFGTHSEASMDYPDTG
                     TPAARAVANGLCDKGILICGSGIGMSITANKIDGIRAALCWNTDLARLSRMHNDANIL
                     VLAGRFTAAPYAIDIAKTWLSTSFEGGRHQNRIDKLIKIEGERS"
     gene            7182..8474
                     /gene="glyA"
                     /locus_tag="PLO35_06545"
     CDS             7182..8474
                     /gene="glyA"
                     /locus_tag="PLO35_06545"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOH79361.1"
                     /translation="MKHIQMNDPELYAAMAAELARQRENLELIASENFTSLAVMEAQG
                     SVLTNKYAEGYPYRWSKKTGQINYKLYGRYYGGCEHIDEVERLAIERAKQLFGADHAN
                     VQAHSGSQANMAAYFSLVKPGDTVLSLELAHGGHLTHGHPLSFSGQLYKIIHYNVNKD
                     TQQFDYEEIEALAKEHKPQMILTGASAYPRKIDFKRFREIADMVGAKLLVDMAHIAGL
                     VAAGLHANPVPYADIVTTTTHKTLRGPRAGLILCKEEYAKIVDGNVFPGIQGGPLMHA
                     IAGKAAAFHEALQPSFKAYQQNVIDNAQALAHALMDEGFKLVSGGTDTHLMLVDLGSE
                     EEGGPSGKKMEESLDLAGITANKNTVPFDTRSPFVASGIRLGTPSVTTRGMGAAEMKQ
                     IAAFIKRVRDNSENEEYLAAMKAEVRDLTSRFPLYPEL"
     gene            complement(8637..10067)
                     /locus_tag="PLO35_06550"
     CDS             complement(8637..10067)
                     /locus_tag="PLO35_06550"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79362.1"
                     /translation="MHPRQLIYLFVILLLLLAACERNTKPEVIAAPLISPQGGEYTPE
                     QLISITCSSPGAVIHFTMDADDPEHNSPLYTSALRLADILPQISNRVTIKARAFKQDL
                     GWSPTTTASFRVNYPNTVERPVITPDAVAQELGAEVLMAVSDPDAQIRYTLDGSLPTY
                     RSNLYTGPVHISRGGYQTVMAAAFKAGWNPSLDAWREYFIPLPGPPLLLVQGGSFVHG
                     PANVSLNSFYISPHELIQSHYYAIMNAGMPYIPIEISAPSPGHKAPTQDARLEYPAYY
                     LSWFDAIEYCNRRSILENLEPCYSYTDPQIGFTAYPETIYPHYWPQGWNQDPANQNKI
                     SCAWDANGYRLPTEAEWTFAARGGNLSVGNDYSGHRWVDPCAWFNLNAGHSTQPIGLK
                     LRNELDLYDMSGNLSEWCWDLLGSLPEGSLSNPTGPSTGYLRVLKGGSWYSDSLACMV
                     VSRAAISPATTNAASVGMRVVRSASR"
BASE COUNT         2259 a         2819 c         2842 g         2280 t
ORIGIN      
        1 ttgaaaccca cctcaaggat ttccttttcc agccctccat ccgatttaca cccccacgtt
       61 ccactacgct gcacaggggg ctctgaaaat gggtttcggg gttgcgtgtt ctatctgaac
      121 tggcgccatg acagggctaa atgcgccatt ttcgcacaag ctcctccacg ggaatgacaa
      181 gtgaaagagc ttttcaatca gacgccaggc tggttcttta cgcccaagct ctgcagtcgg
      241 cgcttcctga atagcttata atctcatatc tcattgtaaa acatagatca tccctctccc
      301 ttcaatgtaa ctcactgccc cactttgacc agcagtatca tcaaccagta gggcttcggt
      361 cttgaaatgg cgttgttgag cagattgcag gccttggaga cgggatttga tctatacaca
      421 aaggtgtcgc ttccgcagcg gggacacaac accggtgcgg caggatggaa gtattggcca
      481 cccaggtgat ttctgacccg cagcaacagg ggatgatagt ctttggaagt gggcccaaag
      541 cacagcgaat caagcttttg agtgccggga aacaggcccc gcagactact gtccgtgaag
      601 ctgtgcagat ggccataggc gtgataaacc tcggcgcaaa ccttgcactt caccatcgat
      661 ttgcctagtt gctcgcggtt tgggaccgag atcagcagat actttctggc taccctcacc
      721 atttcgcgag ccgctttttc aaggtcgggg gtgctcagat gttccagcat ttcgctgctg
      781 aacgccaggt cgaagctgtt gtccgcaaag gggatatcgg tggcggaggc gagcagtttg
      841 ctcactttga ccgctttcaa agccgtttct gaaatatcga tcgccgtcac ctcgtactct
      901 gaggccaaag cattgcagat aataccgttc ccgcatccta cgtcgataat ggtacccgcg
      961 tcttccggga tccattttcg gatggttgcg agcttggcgt cgatctgccc gctgctgtca
     1021 taatcctgcc agatggcgtc atttatctcc atgctgattt cctcgctact ttcatggccg
     1081 catcctgaat tatggcccgt gtttgtcaag ctctttccca gcgtgaaatc cacatcggtg
     1141 aaggacgata aagctgcaat aaacatgaag gtagccaatc agagaggcgg atgatgagct
     1201 ggcatatctg ggatgtttat cgagtcgcat agctatggtg ctgtcccact gcgggttcca
     1261 agttctcgcc ggatccttgc tgagtagcaa tacggagtca ttacggactc attacggact
     1321 tagtccgtaa tgagtccgta ataggatgcg gggaggaagg ggaacggatg ttgcaaggta
     1381 taggtatata aggtaattgt agcaaatcgg gtatggtgtg ggtggattgg aatgattgtc
     1441 tttggatctc aatgatcccc gcggagttgg tgtggacgag cactggcccc gttggagttc
     1501 aatcggccac gataacggtt tgcatgacaa cgagttcgag ggccgcttgg acttcaacca
     1561 gtccaccgta aggacactcc cgctccgaag cactgcttga agtccaaagc ctcgtcaggc
     1621 aggcagataa acgtatcatc tatacagggg ctttggactc caagatggca gaggctggca
     1681 tttctgccag cctcgggtta tcgtaactct tttcttttta gatcattgtg aaaagccggt
     1741 cattcagccc agctgtttga tcacggcttt gccaggaaag atggctgcgg agcccagctc
     1801 ttcttcgatg cgcaggagct ggttgtattt gtccactctt tcgctgcggg atagggaccc
     1861 ggtcttgatc tggccggtat tcatggccac ggcgaggtcg gccaggaagg tgtctccggt
     1921 ttcgccgctg cgatgtgata ccacgcaagt ccagccggct ttgtgggcgg tattgatggc
     1981 gtcgatggtc tcgctgacgc tgccgatctg gttgagtttg atcagcacgc tgttggcgat
     2041 gtcttccttg atgccgcgtt tgatgatggc gggattggtc acaaagacgt catctcccac
     2101 gatctggatc ttcttgccca ggcgctcgtt catcattttc cagccgtccc agtcgttttc
     2161 agccaggccg tcttcgatgg agacgatcgg atacttgcca atcatgcgct cgtaataatc
     2221 gatcatccag gcactgtcac gctgctgtcc ctcaaagaga tagccgccgt ccttccagaa
     2281 gctggaggcc gcggcgtcca aagcgatgaa gatatccgta ccgggtttat aaccagcggc
     2341 gctgatggct tccacgatca cctgcagggc ctcttcgttg gagcccagat tgggagcaaa
     2401 gccaccttcg tcacccacgg aggtcaccag tcctcttttc ttgaggatgg atttgagcgc
     2461 gtggaaggtt tcggcgttca tgcggatggc ctcacggaag ttgggcgcgc ccagcggcat
     2521 gatcataaac tcctggatgt ccacgttatt atcggcatga gcaccgccgt tgatgatatt
     2581 gctcatgggc acgggcaggg tgacggcatt gacgccgccc agataacggt agaggggaat
     2641 gtccagttca atagcgctgg cacgggctac ggccatggaa acggccagaa tggcattggc
     2701 gcccagcttg tctttgttgg cggtgccgtc cagcttgagc atcagctggt cgatggcata
     2761 ttgctgggag gattcgttgc cgcaaagctg gggggcgatt tcatcgtcta tattggccac
     2821 ggctttgagg gtgcctttgc cctgatagcg tgatttatcg ccatcgcgca gctccagagc
     2881 ttcacgctcg cccgtgctgg ccccgctggg aacggcggct ctggccacca caccgctttc
     2941 cagatggacg tccgcctcga cggtgggatt gcctctggaa tccaggatct cacgaccctt
     3001 gatatacata atttcagaca tgttttgtat ctccttgtgt gtatagtatc aagttacgcg
     3061 ttacgcggta agcgtgatgc gatcgtaacg cgtcacgggt aacgcgtcat gggtaactta
     3121 cttttcaaat atattggcca gggttttcac tgatcctatc aaagcggcag attcataggg
     3181 gatgaccaca gtcttgtcac cctgtttggt gatttcctga aaagcggcca cgtatttgag
     3241 agcgatcagg tattgagcgg gatcggtctt ggaatccttc accgcttccg cgatcatctg
     3301 gatcgatttg cgttccgcgt ccgccaccaa aagcttggcc tgggcctcac cttcggcccg
     3361 ggcgatcttg gcctgtttgt caccttccgc cacgcggatc tggaattccc ggtcggcatc
     3421 acccgtgagc attttggcac gtttgtcgcg ctcggccctc atttcctttt ccatcgcggt
     3481 gcgaatctct tcgggaggca ggatctcctg cagttccacg cggttgacct tcacgcccca
     3541 tttatcggta gcgtcgtcca gaatgtcgcg cagtttggca ttgatggcat cacgggaggt
     3601 gagggtgtgg tccagggtga gctcaccgat cacgttacgc agcgaggtct gggtgagctt
     3661 ttcgatggcc tcggggaggt tgccgatctc gtaaacggat ttgtaggggt cggtgacctg
     3721 gaaatagagc agggcgttga tgttgatgga gacgttgtca ctggtgatca cgttttgacg
     3781 cgggaagtca tagaccgtct cgcgcaggtc gatccggttt tccacccgct gcacctggat
     3841 ggtctggcca cggtagtccg tcttgttgta gcgccagtgg atgggcctgg tcttatcgaa
     3901 caccggcacg atgatatgaa tgcccgattg gagggtggtg tagtactttc cgaggcgttc
     3961 gatgatcaca acttcagcct ggcgcaccac gataatcccc ttggccacga tcacaaccac
     4021 aaagatggcg atagcgatca taacccatag gccggtagca acggattgac ttacaagcaa
     4081 taaactgaca ggtaacatac tggactcctt tttcgcgtaa tttggtgctc attttatcag
     4141 ccccggagtt ctggcaagca aaaaagccat ccatgctttc aaggccagaa ctttgggctt
     4201 gacaaatatt tagtgttttt aaaaccatgt ttggacggag cggatcgact ccctccgttt
     4261 cattaaaatc ggcaactgcc gataatataa caatttcagc ggataacaca atgagaataa
     4321 ctatcctttc agtcctgtcc ctaatgctga ttctgtcggg ttgcgcgttc cgcaagcggg
     4381 agcgtcctga tccggacagc gggattggtc tgagcggaag tttcagcgcc aaggattcac
     4441 ccctggtagc tcgggagatc agcctggaga tcacctccca tccctggctg cagcagtttc
     4501 acggctcccg ccagcgcagc cccgtgatca tgatcgggat ccttacaaat gagtcctacc
     4561 agacgatcga tatggacctc tttggcagac agctcatgca tgagatcctg aacgcgggtg
     4621 agctaaggtt tgtggaagaa cttccggtgc cgctcagaga gcgcagtgaa ccatccctgg
     4681 acctggccag agaactggcg gcggattttt atctgacggc catcgtccgc accgccgagc
     4741 agcagtataa aagcaagcgg atcaggtatc tgcagctgga tatgaagctg ttggaagtag
     4801 ccacggggca gcctgtctgg acgattctga ggcccatccg gcaaaacccc acccagggag
     4861 ccgcgcagtg agctatcatg cacttatact attgaattac ataaatatat aaactggaga
     4921 cacagtatgc aaatcaccaa gcttgaacag atgttagaag tgctcaagtc gcgccataaa
     4981 aagcgtctcg tggcagcctg ggccatcgat gcccacacca tcgtagccgt cagtgaagcg
     5041 gtcggaatgg gtattatcga tgccatcctg gtgggtgacg aagccatcat caaagacgtt
     5101 tgcgcccagc atggcatcga tccctccatc ttccggatcg tgagtgccat ttccgatgtt
     5161 gacggagctg ccaaagccgt ggaactcatc aatgccggcg aaggtgactt tctgatgaag
     5221 ggcctcatca gcacggaccg ctatatgaaa gccatcctca acaaagaaaa aggcctgatg
     5281 gaaaatgggg ctatcctggc ccatgtgacc gtcgccgaac cgatgggata tcacaaactg
     5341 ctggtgtttg gagacgtagc cattattccc gagcccgatt tgaaccagaa gatcgccatc
     5401 tgcaactatc tgatcaagac cgccaaactg ctgggcatcg acacccccaa gctgggcatc
     5461 ctttccgctt ccgaacagac cctgcccaag atccgctcct cctatgaagc ggccctgata
     5521 tccaagatgg ccgaacgcgg tcagatcaaa ggtgcgatcg tggacggacc tctgggcttt
     5581 gacctcatca tcgacaagca atcggccgag atcaaaggcg tgaaaagcga ggtctgtgga
     5641 gacgcggact gcatcctctt ccccaatata gaagccggaa acactttcta caagaccatc
     5701 acttgcctga tgggctccga gctttgcgcc atcgtgatgg gcgccaaggt accggcagtg
     5761 ctcacttcca gaggtgattc cgagcgcagc aagctctatt ccatcgccct ggcggctatc
     5821 ctggcatgat atgaaagcga tcaccaaact cagtgaactg gcctctcacg ttcgcgaccg
     5881 aggttttaag gcccgggtag ccgtggcggc agcagaggat caaaacaccc tctcctccat
     5941 cgccagagcc gtttccgaag gctgggcgca tgccatcctg catggccgcc aagccaggat
     6001 tgaagaggtc tgccgcgccg aaggcctgga gccctccctg tttgagatca tcgaccatcc
     6061 cgatgagctc agcgccacgc tggaggctac ccgccaggtg agagaaggcg aagcggatgt
     6121 gctgatgaaa ggcctggtgg gcacggacaa gtttctgaaa gccgtgctgg acaaacaaaa
     6181 aggcctgctt cctcccggag ccgtgatgag ctatacctgc gccctggaac tgccaaagta
     6241 tcccaagttg ctctttgttt cagatacagc ggtgctgccg gatcccgatc tggcccagac
     6301 tcaggctatg atcaactaca gcgtggccat ggccaggcgc tttggcatcg agtgtcccaa
     6361 ggtggccctc atctccgcca ctgaaaaggt ggggccttcc atgccgtcga ccctggaaca
     6421 cgcgctgctc tgcaagatga atcagcgtgg ccagatcaag aactgcctca tcgacggccc
     6481 tctggatatc ttcctggcct gcgatcccgc cgctggagag atcaaaggcg tcaagacacc
     6541 catcaatggc gaggcggatg tgttgatctt ccccagcctg gaaagcgcca acagctttta
     6601 caaaggcctc atgctctttg ccggagctga actggccggc ctcattcaag gcaccgtcaa
     6661 acccgtggtg gtgatgagcc gcagtgaaag tgctgccagc aaatactact gcgtggccct
     6721 ggcttgcctg atggcggaag agcaatgaaa ctggcaatcg cttccgatca cgccggttac
     6781 gctctcaaag aagccctcaa gttcgctttc ccggagcatg aatgggtgga ttttggtact
     6841 cattccgaag cctccatgga ttatcccgat accggtactc ctgccgcccg ggcagtcgcc
     6901 aacggactct gcgacaaagg catcctgatc tgcggcagcg gcatcggcat gagcatcacc
     6961 gccaacaaga tcgacggcat cagagcggcc ctgtgctgga ataccgacct ggcacgtctt
     7021 agtcgcatgc acaacgacgc caatatcctg gtcctggccg gcagatttac cgccgcgcct
     7081 tatgccatag acatagccaa aacctggctc tcaacctctt tcgagggggg gcgccaccaa
     7141 aaccgcatcg ataaacttat aaaaatagaa ggagaaaggt catgaaacac atccagatga
     7201 atgaccccga actgtacgcc gccatggcag ccgaactggc ccgccagcgc gaaaacctgg
     7261 agctcattgc ctccgaaaac ttcacttccc tggccgtgat ggaagcccag ggcagcgtgc
     7321 tcaccaacaa atacgctgaa ggctatccct accgctggag caaaaagacc ggccagatca
     7381 actataaact ctatggccgc tactatggcg gctgcgagca catcgatgaa gtggaacgcc
     7441 tggctatcga acgcgccaaa caactctttg gggcggacca tgccaacgtg caggcccaca
     7501 gcggttccca ggccaatatg gccgcctatt tcagcctggt aaaacctggc gacacagtgc
     7561 tttccctgga actggcgcac ggcggtcacc tcacccacgg ccatccgctt tccttttcag
     7621 gacagctcta caagatcatc cactacaacg tcaacaaaga cacccaacag tttgactatg
     7681 aagagataga agccctggcc aaagagcaca aaccccagat gatcctcacc ggcgcttctg
     7741 cctatccccg aaagatcgat tttaaacgct tccgcgaaat agcggatatg gtaggcgcca
     7801 agctgctggt ggatatggcc cacatcgctg gactcgtggc ggccggactg cacgccaacc
     7861 ccgtgcccta tgccgatatc gtgaccacca ccacccacaa aactctgcgc ggtccgcgtg
     7921 ccggactcat cctctgcaag gaagaatacg ccaaaatcgt ggatggcaac gtgtttccag
     7981 gcatccaggg cggtcccctc atgcatgcca tcgcagggaa agctgccgcc ttccatgaag
     8041 ccctgcagcc ctccttcaag gcctatcagc agaatgtgat cgacaacgcc caggccctgg
     8101 cccatgccct tatggatgaa ggctttaagc tggtctcggg aggcacggac acccacctca
     8161 tgctcgtcga tctgggcagc gaggaagaag gtggccccag tggcaaaaag atggaggaat
     8221 ccctcgatct ggccggcatc actgccaata aaaacactgt acctttcgac acccgcagcc
     8281 cctttgtggc ttcagggatc aggcttggca ctccctccgt cactacccgt ggcatgggtg
     8341 ccgccgagat gaaacagatc gccgccttca tcaagagagt gcgcgacaac agcgaaaacg
     8401 aagaatacct ggccgccatg aaagcggagg tgagagacct cacatcccgc ttcccgctct
     8461 atcccgaact ctaaaaccgc aaagccgttc tgtatgcagc gccgggcctt tggtccggcg
     8521 ctttttggta ggcttggatg cagattcgac acctgtgtga ggtggaaaag cacctggcat
     8581 cagtttttca cgaagcagtt acgcgcaccc tatatggatc gcgacctatc ataatactaa
     8641 cggcttgcgc tcctcaccac cctcattccc accgaagcgg cattggtggt ggcgggactt
     8701 atcgcggccc ggctgaccac catgcaggcc agggaatcgc tgtaccaact gccccccttg
     8761 agcactctca aatagcctgt ggatggcccg gtgggattgc tcagtgagcc ctccggaagg
     8821 ctgcccagga ggtcccaaca ccattcgctg aggttgccgc tcatgtcata caggtccaat
     8881 tcattgcgca atttgaggcc aataggctga gtgctgtggc ccgcgttgag gttgaaccag
     8941 gcgcaggggt ccacccagcg atgaccgctg taatcgtttc ccacgcttag gtttccacct
     9001 ctggcggcaa aggtccattc cgcttcggtg ggcagtctgt aaccgttggc gtcccaggcg
     9061 caggagatct tgttctgatt ggcggggtct tgattccagc cctgcggcca gtagtgggga
     9121 tagatggttt ccggataggc ggtgaaccct atctgggggt cggtgtagct gtagcagggc
     9181 tccaggtttt ccaggatgct gcggcggttg cagtattcga tagcgtcaaa ccaggaaaga
     9241 taataggccg ggtattccag tctggcgtcc tgcgtggggg ctttatgacc tggggacggg
     9301 gcacttatct cgatcgggat gtagggcatg ccggcattca tgatggcgta gtaatgggac
     9361 tgaatgagct catgagggct gatgtaaaag ctgttgaggc ttacgttggc gggaccgtga
     9421 acaaaactgc cgccctgcac caatagcaga ggcggacccg gcagcgggat gaaatactcc
     9481 ctccaggcat ccagactggg attccagccg gctttaaacg cggccgccat cactgtctgg
     9541 tagcctcccc gggagatatg aacgggaccg gtgtagaggt tggagcgata agtgggaagg
     9601 ctgccgtcca gggtgtagcg gatctgggca tcgggatcgc tgacggccat caggacctcg
     9661 gctcccagct cctgcgcgac cgcatccgga gtaatcacgg gccgctcgac agtgttggga
     9721 taattgactc tgaagctagc cgtggtcgtg gggctccaac cgaggtcttg cttgaaagct
     9781 ctggccttga tggtgacccg gttgctgatc tggggcagaa tgtcggccag cctcaaagcg
     9841 ctggtgtaga gaggactgtt gtgctccgga tcgtccgcgt ccatagtaaa gtggattacc
     9901 gcgccggggc tggaacaagt gatcgagatg agctgttccg gagtgtattc acccccctgg
     9961 ggagagataa gaggcgcggc aataacctct ggtttggtgt tccgctcgca ggccgcgagc
    10021 agtagcaaga gtatgacaaa caaatagatg agctggcgtg ggtgcatggc tggctcccct
    10081 tttgagctta tttcggcagg atttattatg gagcaataat tgttccaggc ctggcgattc
    10141 gaattgagta ttgaggatgt atggaacgca gaaaaaggga aaaaaggatt tacgcagatt
//
LOCUS       DAOSFN010000087        10179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5611_length_10179_cov_8.160707, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000087 DAOSFN010000000
VERSION     DAOSFN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5611_length_10179_cov_8.160707"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1601)
                     /locus_tag="PLO35_06555"
     CDS             complement(231..1601)
                     /locus_tag="PLO35_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH79363.1"
                     /translation="MRRGMRISVIEGIFAQVYGSLCQIGSGFITKFMVILGATPLQFS
                     LLSALGQVSALFQPLGVAFTHHLRRRKWACVWITAAGRFLTFFMGAALLFASSRMGIW
                     FLLALLFVSMALQSIGANIWIAWVSDLIPLRIRGRFFSRRNQILLLAGLVVGYGVSLY
                     VDMFEKQGGLARRVLVESLGLERWFTVANQPWFLSGVFIAGTLLSLIGLIVLSRQPER
                     PLRSHAQGRLRDEYKRPFQDPNFRKLLFFGVWWMLAIGVGSAFWGPFMLKKLEMGMFR
                     MQLYGSLHAAASLVAYNFWGRFIDRFGNKTAMKICVALGGLNPMLWLFTAPGSYGILW
                     FEAILSGSMWAGNAIVTTNFVLSIAPRGKQQVYSGLYGAIAGVSMMASTLLSGVFFPS
                     SLDLGFRILEPEQVVFGLGGLLRWLAIIPLLGVVEKHTVPLRQALYGTLRSTLENVFS
                     FFTWRK"
     assembly_gap    1645..1744
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2091..2912)
                     /gene="rnc"
                     /locus_tag="PLO35_06560"
     CDS             complement(2091..2912)
                     /gene="rnc"
                     /locus_tag="PLO35_06560"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOH79364.1"
                     /translation="MTQTEKTTPVIGPGERNGLKRIIARIVEYIGGKKIGEQYPKWEK
                     SLVQLQKRVDYNFHDCDLLRAALTHKSYLRRKFDDHQTPSPFERMEFLGDSILGFVVS
                     KELFSRHPDEQEGKLSKLKSKIVSETYLTLKANALELGKYLLLSPEEQQSGGAKKPSI
                     LSDSMEAIICAIYLDSGIASATRFIRNHILKDYESTVTRNELVNYKSMLQEHMQSRNQ
                     EPPRYLTIAEEGPEHNKTFVVEVYQGDKLLGTGKGNTKKNAHQDAAHQACQKLGI"
     gene            complement(2920..4158)
                     /gene="fabF"
                     /locus_tag="PLO35_06565"
     CDS             complement(2920..4158)
                     /gene="fabF"
                     /locus_tag="PLO35_06565"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOH79365.1"
                     /translation="MKRRVVITGMGTVSPVGNSVQESWDSLVNGRSGVGLITRFDASS
                     LPTHIAAEVKNFKPEEHFDPKEARKLDIYTQFALLAAREAVSDAGLKEFAFDPDRVGV
                     ITGVGIGGIITFEEECIKGYTQGPRRISPFFIPKMIGNIAAAHIAIENNFKGPNFNVM
                     SACASANHAIGTAYRAIQYGDADIIVSGGTEGGVSSLAVGGFCAMRALSTRNDDPTAA
                     SRPFDKDRDGFVMSEGGAFLILEELEHAKARGAKIYAELAGYGATDDAFHITAPTEDG
                     EGSARAMIAAMKDAGLQPEDIQYINAHGTSTPLNDKGETQSIKTAFGDYAYKLRINSS
                     KSMVGHMLGAAAGFEAIVCVKTIETGIIHPTINLDNPDPLCDLDYTPHQAVRQEVEAA
                     LSNSLGFGGHNAALVIKRYR"
     gene            complement(4314..4547)
                     /locus_tag="PLO35_06570"
     CDS             complement(4314..4547)
                     /locus_tag="PLO35_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH79366.1"
                     /translation="MDIEAKVKQIVMEKLNVEEAQIVPEANFIDDLRADSLDTVELVM
                     AFEDEFKISIPDEDQDKLRTVGAAVDYLKEKLG"
     gene            complement(4614..5363)
                     /gene="fabG"
                     /locus_tag="PLO35_06575"
     CDS             complement(4614..5363)
                     /gene="fabG"
                     /locus_tag="PLO35_06575"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOH79367.1"
                     /translation="MNYDMTDKVTVITGSARGIGFALATAFGAAGAKVMVIDLAPEAV
                     EAAVQKLRDQGSQAWGYVGNVTDSAMMEELFARVVAEHGSLDVLINNAGVTRDNLMLR
                     MKEEEWQLVMDINLKGSFICTQKAFKHMMKARKGSIVNIASVIGLMGNAGQANYAASK
                     GGMIAFTKSCAKEFASRGVRVNAVAPGFIETEMTASLPADVVAAYAKAIPLAKMGSPL
                     DVAKLCLFLASNESAYITGQTIAVDGGLTMQ"
     gene            complement(5389..6321)
                     /gene="fabD"
                     /locus_tag="PLO35_06580"
     CDS             complement(5389..6321)
                     /gene="fabD"
                     /locus_tag="PLO35_06580"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOH79368.1"
                     /translation="MKTAFVFPGQGAQYVGMAMDYLAAFPEFDQELASFDAAHGTSLR
                     EIMASGPEDALKETRFTQPAILFHSLCALKAFEAKSHIRPDFVAGHSLGEFSALVASG
                     ILSWQDALHLVHKRGEFMIKANAGVPFAMAAVIGLDADAVIGACEEANQTGLVMAVNF
                     NTPIQTVISGSASGVAKAGEILKAKGAKRVLPLVVGGPFHTPLIQQASGWLASEMAKA
                     DFRDGRVPVISNLDAFPSTSGSEARAKLERQIISPVLWVQSVQAMLEAGVTRFVEFGP
                     QKVLSGMIKNIDKDAEVWNLDKLEELDALTGALS"
     gene            complement(6342..7334)
                     /locus_tag="PLO35_06585"
     CDS             complement(6342..7334)
                     /locus_tag="PLO35_06585"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOH79369.1"
                     /translation="MATFHAKFSSFGSYAPKKIVNNFDLEKVVETTDEWIRSRTGMFE
                     RHYCTESEAASDLAHQAALNAIEASQVKYRDIDLIIVATVTGDHAFPSTACVVQRRLG
                     LMHVPAFDISAGCTGFVYALDMARQYVENGSKRNVLVIGVDVLTKITNWQDRNTCVLF
                     GDGAGATIISRAESSDISRIIDSFIDADGSMGDLLIQQGGGSRLPASHETVDKNLHTV
                     YMEGNKIYKNAIKSMFAACDELLRRNKMTVSDVDWLIPHQANLRIIEGLADKMKAPMS
                     KVIVNIEKYGNTSSGTIPLAADEAIRAKKVRRGDIILLTSFGAGLTWGAVLARY"
     gene            complement(7334..8332)
                     /gene="plsX"
                     /locus_tag="PLO35_06590"
     CDS             complement(7334..8332)
                     /gene="plsX"
                     /locus_tag="PLO35_06590"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOH79370.1"
                     /translation="MRVAIDAFGSDKAPYPEVEGAVMAVKEEVCDEVILVGDESILRP
                     ELEKFFHDKQRVKIVHASQRITMDEHPATAVRSKKDSSMLRAVELHKSGEADAAVSAG
                     STGAFMAASLLVYGRIKGVSRPAIAITFPTQKHHEIILDVGANVDCDARNLLEFAHLG
                     RIYYKYFFGAEEPRVSLLNIGEESAKGNTVSREAHKLLAEAKDLNFIGNIEGKDVLKG
                     ITDVIVCDGFVGNVMLKTVEGAIISIFDILKEQFNKDWIAKFGAMLSYPVYSYLKKKL
                     DHTEYGGALLVGLNGTSIVSHGRSNAKAVKNAIRAAARISRSGFVQHSKEYFERFV"
     gene            complement(8334..8513)
                     /gene="rpmF"
                     /locus_tag="PLO35_06595"
     CDS             complement(8334..8513)
                     /gene="rpmF"
                     /locus_tag="PLO35_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOH79371.1"
                     /translation="MAVPKRKTSKTRRDKRRTHDALTPPAVSTCSKCGEPKRPHHVCE
                     KCGTYDGKQVLTVKE"
     gene            complement(8948..9784)
                     /locus_tag="PLO35_06600"
     CDS             complement(8948..9784)
                     /locus_tag="PLO35_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79372.1"
                     /translation="MLNILLSILCSVGIANLLMLFGKLKRHADPDPRHAMLPVFLGNY
                     IMATVFSYLAVPRGGFQLHAFDLGLGIAAGALFLTNFWVYQRSIVINGLAISVSVMRV
                     AMVLPVLVSLLIFAERLKPLNFAGIALAILAFMLKPGGEGRRHLVWLIALFAVSGLTD
                     LSLKLFKELGSGSEALWIGMVFSSALIFTAALIIIRKVKLGWLPLLFGFGLGIPNRLS
                     TVFFLKGLATVPAAVAYPVTASGIVLVSVISDALFWKRIPVRRELLMYALLALGLILL
                     NL"
     gene            complement(9941..>10179)
                     /locus_tag="PLO35_06605"
     CDS             complement(9941..>10179)
                     /locus_tag="PLO35_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79373.1"
                     /translation="EFCLPLSDPSGNGYIRGIGIAIPLSDLPTKKQVADHAPFLYDYW
                     SDVDDPDMAIYEIRIKSFAYLKPGDMLENKVTLN"
BASE COUNT         2151 a         2692 c         2923 g         2313 t
ORIGIN      
        1 gtgaacagag aacgcgctga ggtggagcgg caaacgtgcg cgtttggacg gttcgcgtta
       61 cgcggggggc gcgccgatat ggaaatcgac gctacaaccc ctccgaaaca ggacgtttcc
      121 gactacacaa gtgagagcat tctcaatcct caattgtcaa ttctcaataa acaaggagcc
      181 cgatcatctc ttttttccct ttttcagcgt tctattcatt ctcaatagcc tcatttcctc
      241 caggtaaaga agctgaacac gttttccagg gtggaacgga gcgtgccata cagcgcttgc
      301 cgcagaggta ctgtatgctt ttccaccacg cccaaaagcg gaatgatggc cagccagcgc
      361 agcaagcctc ccagcccaaa gaccacctgt tccggctcca ggatcctgaa tcccaaatcc
      421 agtgaagagg ggaaaaacac tcccgagagc agggtcgagg ccatcatgct cactcccgcg
      481 atggcaccgt ataaaccact gtaaacctgc tgtttgcccc ggggcgcgat ggacagcaca
      541 aagttggtgg tcacaatggc gttgccggcc cacatcgatc cggagaggat cgcctcaaac
      601 cacaggatgc catagctgcc gggggccgtg aagagccaca gcatgggatt caagcctccc
      661 agcgccacgc agattttcat cgccgtctta ttgccaaagc ggtcgatgaa gcgtccccaa
      721 aagttgtagg ccaccagaga ggccgcggcg tgcagactgc cgtagagctg catccgaaac
      781 atccccatct ccagcttttt gagcatgaag ggcccccaga aggcacttcc cacgccaatg
      841 gccaacatcc accagacgcc aaaaaagagc agcttgcgaa agttggggtc ctgaaagggc
      901 cgcttgtatt cgtctctgag gcgtccctga gcatgcgatc tgaggggacg ttccggctga
      961 cgggacagca cgataagccc tatcaaggag agcagcgtgc ccgcgatgaa gaccccactc
     1021 agaaaccagg gctgattggc aaccgtaaac cagcgctcca gccccaggct ttccaccagc
     1081 acccgtctgg ccaggccgcc ttgcttttca aacatatcca catacaggct cactccatag
     1141 cccaccacca gtccggccag cagcaggatc tgattgcgcc gggaaaagaa gcgtcccctg
     1201 atccggagcg ggatcagatc gctcacccag gcgatccaga tattggcacc gatcgattgc
     1261 agggccatgc tcacaaacaa cagcgccaac agaaaccaga tgcccatccg ggagctggca
     1321 aagagcagcg cggctcccat gaagaaggtg agaaagcgtc ccgccgcggt gatccacacg
     1381 caagcccatt tgcgccgccg caaatgatgg gtgaaagcca ctcccagagg ctgaaacaac
     1441 gccgagacct gtcccagcgc gctaagcagc gaaaactgca gcggggtggc ccccaaaatc
     1501 accatgaact tggtgataaa tccgctccca atctggcaca aagagccata cacctgggca
     1561 aaaatgccct ctatgacgga aatacgcatc cccctccgca ccagatgacg gtgacggtga
     1621 tcgggagcga tatctctgtg tgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnattgag gattgagaat gaatagaacg ctgatgacac tgattaaact gatttacaca
     1801 gattcttttt tatagaacgc agaaaaaggg gaaaaaggga tgatggggag gattgaggat
     1861 tgaggattga gaattgagga ttgagaattg aggattgagg attgagaatt gagaattgag
     1921 aatggataga actcagatga ccggatttaa atgatctcta aaaccctcat cctcagtcaa
     1981 tcaaatccgc gttaatcagc tcaatctgtg tcatcagcgt tctattcgtt aaatccagga
     2041 tcctgataat cccttttttc tctatttctg cgttctattt gatgatcagt tcagatgccg
     2101 agtttctggc aggcctggtg ggcggcgtcc tggtgggcgt ttttcttggt gttgcctttg
     2161 ccggtgccca gcagtttatc tccctgatag acttccacca cgaaggtttt gttgtgttcg
     2221 gggccttctt cggcgatggt caaatagcgg ggcggttcct ggttgcggct ctgcatatgc
     2281 tcttgcagca tgcttttata gttcaccagt tcgttgcggg tgacggtgga ttcatagtct
     2341 ttgaggatgt ggttgcggat gaagcgggtg gcggaagcga tgccggaatc cagatagatg
     2401 gcgcagatga tcgcctccat gctgtcgctg aggatggagg gttttttagc accgccggac
     2461 tgctgttcct cagggctgag cagcaggtat tttcccagct cgagggcgtt ggccttgagg
     2521 gtgaggtagg tctcggagac gatcttggac ttgagcttgc tgagcttgcc ttcctgttcg
     2581 tccggatggc ggctgaagag ctctttggag accacgaagc ccaggatgga atcgcccaaa
     2641 aactccatgc gctcgaaagg cgagggagtc tgatggtcgt caaacttgcg gcgcagatag
     2701 gatttgtggg tgagggcggc gcgcaggagg tcgcaatcgt gaaagttgta atcgacgcgt
     2761 ttctggagct ggacgaggct cttttcccac ttgggatact gctcgccgat ctttttgccg
     2821 ccgatgtatt ccacgatgcg ggcaatgatg cgtttgaggc cgttcctctc ccccgggcca
     2881 atgactggcg tagttttttc tgtctgggtc ataaactgct cagcgatagc gtttgatcac
     2941 cagggcggcg ttgtgtccgc cgaagcccag tgaatttgag agggccgctt ccacttcctg
     3001 ccggacagcc tggtggggcg tgtaatccag gtcgcagagg ggatcgggat tgtccagatt
     3061 gatggtggga tggatgatgc cggtctcgat ggtcttgacg cagacgatcg cttcaaagcc
     3121 agcggccgcg cccagcatgt gccccaccat ggatttggag gaattgatcc tgagcttgta
     3181 ggcataatct ccgaaggcgg tcttgatgga ttgggtctcg cctttgtcgt tgaggggagt
     3241 ggaagtgccg tgggcgttga tgtattggat gtcttcaggc tgcaggccgg cgtctttcat
     3301 ggccgcgatc atggccctgg cgctgccttc accgtcttca gtgggagcgg tgatgtggaa
     3361 ggcgtcgtcc gtagcgccgt atcctgccag ttcggcatag atcttggcgc cgcgggcttt
     3421 ggcgtgttcc agctcttcca gaatcaggaa agcgccgccc tcgctcatca caaagccgtc
     3481 gcggtcttta tcgaagggac gtgaggccgc ggtgggatcg tcgttgcggg tggaaagggc
     3541 ccgcatggca cagaatccac ctaccgccag agatgatact ccgccttcgg tgccgccgct
     3601 cacgatgatg tcggcatcgc catactggat ggcgcgatag gcggtcccga tggcatggtt
     3661 ggcgctggcg caggcgctca tgacgttgaa gttggggcct ttaaagttgt tttcgatggc
     3721 gatatgggcg gcggcgatgt tgccgatcat tttggggatg aagaagggac tgatgcggcg
     3781 cgggccctga gtgtagcctt tgatgcattc ttcttcgaag gtgatgatgc cgccaatgcc
     3841 cacaccggtg atcacaccca cgcggtccgg atcaaaggca aattccttga ggccggcgtc
     3901 actaacggct tcccttgcgg cgaggagcgc gaactgggta tagatatcca gtttgcgggc
     3961 ttctttgggg tcgaagtgtt cttccggctt gaagtttttg acttcagcgg cgatgtgggt
     4021 gggaagggat gaagcatcaa aacgggtgat cagacctact ccgctgcggc cattgaccag
     4081 gctgtcccag gattcctgga cgctgttgcc gacgggtgag acggttccca taccggtgat
     4141 aactactctt cttttcatgt gcaattacct catatagtta acaaacttga gtgccgcggc
     4201 ttgtgttcat ctgaagcatg gcaagccatc gttcagatca agacaggcag gcactataaa
     4261 accgggagca atcctgatgg atggtgccat caggattgtc ccgccttagg gaattaaccc
     4321 agcttttcct tcagatagtc aacggcggcg ccgacggtgc ggagcttgtc ctgatcttca
     4381 tccgggatgc tgatcttgaa ttcatcttca aaggccatga cgagttccac tgtgtccagt
     4441 gaatcagcgc gcaggtcatc gatgaagttg gcttcgggaa cgatctgagc ttcctcgacg
     4501 ttcagttttt ccatcacgat ctgtttgact ttggcttcaa tatccattgt tcctcctatg
     4561 ttattgaagt gtatttttgg gttgtgtatt tcaagggtgg ataccctcgg gttttattgc
     4621 atggtgaggc caccatcgac ggcgatggtc tggccggtga tataggcact ctcgttggag
     4681 gccagaaaga ggcaaagttt ggccacatcg agggggctgc ccatcttggc aagcgggatg
     4741 gctttggcat aggcggccac cacatcggct ggcagggagg cggtcatttc cgtctcgata
     4801 aagcctgggg ccacggcatt tacgcgcacg ccccggctgg cgaattcctt ggcgcagctc
     4861 ttggtgaagg cgatcatacc acccttggag gcggcgtagt tggcctggcc ggcgttgccc
     4921 atcagaccga tcacgctggc gatgttgaca atgctgcctt tgcgggcctt catcatgtgt
     4981 ttaaaggctt tctgggtgca gatgaagctg cctttgaggt tgatatccat caccagctgc
     5041 cactcctctt ccttcatgcg cagcatgagg ttgtcgcgag tgaccccggc gttgttgatc
     5101 aacacatcca ggctgccatg ctctgccacg acgcgggcaa agagctcttc catcatggcg
     5161 gagtccgtga cgttgcccac atatccccag gcctgtgatc cctggtcgcg cagcttttgg
     5221 acggctgctt ccacggcttc gggagccagg tcgatcacca tcactttggc gccagcggcc
     5281 ccaaaagcgg tggccagggc aaaaccgatc ccacgggccg agccggtgat cacggtaact
     5341 ttatcggtca tatcgtagtt catgatttcc tcaattgggt ggattggatc agctcaaagc
     5401 cccggtcagg gcatcgagtt cttccagttt gtcaagattc cagacctcgg catctttgtc
     5461 aatgttcttg atcatgccgc tcagcacttt ctggggacca aactccacaa aacgcgtcac
     5521 ccctgcctcc agcatggctt gcacgctctg cacccacagc accggagaga tgatctgacg
     5581 ctccagcttg gcgcgcgctt cgctgcctga ggtggaaggg aaggcatcca gattggagat
     5641 caccgggacg cggccatctc taaaatcggc tttggccatc tcggaggcca gccagccgga
     5701 agcctgctgg atcaaaggtg tgtggaaggg acctcccacc accaggggta aaactcgctt
     5761 ggcgcctttg gccttgagga tttcaccggc tttggccacg cctgacgcgc tgccggaaat
     5821 cactgtctgg atgggggtgt tgaagttcac tgccatcacc agtccggttt gattggcttc
     5881 ctcgcaagcc ccgatcacgg catcggcatc caaaccgatc accgcggcca tggcaaacgg
     5941 cactccggca ttggctttga tcataaactc gccgcgcttg tgcacgaggt gcagggcgtc
     6001 ctgccagctc aggatccccg aggctaccag agcgctgaac tcgcccagag aatgaccggc
     6061 cacaaagtcc ggccggatat ggcttttggc ctcaaaagcc ttgagggcgc agaggctgtg
     6121 aaagaggatg gccggctggg tgaagcgggt ctctttgagg gcatcttccg gtccggaggc
     6181 cattatttct ctaaggctgg tgccgtgggc ggcgtcgaag ctggccagtt cctggtcaaa
     6241 ctccggaaac gcggccagat aatccatggc catgcccaca tactgagcac cctgtccggg
     6301 aaacacaaag gctgtcttca tattccttaa aactccttgg atcaatatct tgccagcacg
     6361 gcaccccagg tgagcccggc tccgaacgag gtgagcagaa tgatatctcc gcggcggact
     6421 ttcttggccc ggatcgcttc gtcggcggcc aggggaatgg tgccggaaga ggtgttgcca
     6481 tacttctcga tgttgacgat caccttgctc atgggggctt tcatcttgtc cgccaggcct
     6541 tcgatgatgc gcaggttggc ctggtggggg atcagccagt ccacatcgct cacggtcatc
     6601 ttgttgcggc gcagcagctc atcgcaggcg gcgaacatcg atttgatggc gtttttatag
     6661 atcttgttgc cttccatata gacggtgtgg aggtttttgt ccaccgtctc atggctggcc
     6721 ggcaggcgtg atcctccccc ctgttggatc aggaggtcgc ccatcgagcc gtcggcatcg
     6781 ataaaggaat cgattatgcg tgaaatatcg gaagattccg cccgggatat aatggtggcc
     6841 ccggccccat cgccaaagag gacgcaggtg ttgcggtcct gccagttggt gatcttggtc
     6901 agcacgtcca cgccaatcac gaggacgttg cgcttggagc cgttttccac atactggcgg
     6961 gccatatcta gagcgtagac aaagccggtg cagcctgccg agatatcaaa ggccggcacg
     7021 tgcatcaggc ccagccgtct ctgcaccacg caagcggtgg agggaaaggc gtggtctccg
     7081 gtcacggtgg ccacgatgat caggtctata tcgcgatatt tgacctggga ggcctcgatg
     7141 gcattgagag cggcctgatg ggcaaggtcg ctggcagctt cgctttcggt gcagtagtgg
     7201 cgctcaaaca ttccggtgcg gctgcggatc cactcatcgg tggtttccac caccttttcc
     7261 agatcaaaat tgttgacgat cttcttggga gcataagagc cgaagctgga gaacttggca
     7321 tgaaaagtgg ccattacaca aacctctcaa aatattcttt gctgtgttgg acgaagccgg
     7381 aacgtgagat gcgggccgcg gcgcgaatgg catttttgac cgccttggcg ttggaacggc
     7441 cgtgggaaac gatgctggtg ccgttgaggc ccaccagcaa agccccgccg tattccgtgt
     7501 ggtccagctt ctttttgaga tagctgtaaa cggggtagga gagcatcgcg ccaaatttgg
     7561 cgatccaatc tttgttgaac tgctctttga ggatatcgaa aatggagatg atggcaccct
     7621 cgacggtctt gagcatgacg ttgcccacaa agccgtcgca gacgatcacg tccgtgatgc
     7681 ccttgaggac gtccttgcct tcgatgttgc cgatgaagtt gaggtctttg gcctcagcca
     7741 ggagcttgtg ggcctcgcga ctcacggtgt tgcccttggc gctttcttca ccgatgttga
     7801 gcagcgaaac gcgcggctcc tcagcgccaa agaaatactt gtagtatatg cggcccaaat
     7861 gggcaaattc caagaggttg cgcgcgtcgc aatccacgtt tgcccccacg tccagaatga
     7921 tctcgtgatg cttctgagtg gggaaagtga tggcgatagc gggacgagac acccctttga
     7981 tcctgccata caccagcagg gaagcggcca tgaaagcacc tgtgcttcct gcacttacag
     8041 cggcatcggc ctcgccggac ttgtggagct ccaccgctct cagcatagag gaatctttct
     8101 tggaacgtac tgccgtggcg ggatgttcgt ccatggtgat ccgctgggag gcgtgaacga
     8161 tcttcactct ctgtttatcg tgaaaaaact tttccagctc cgggcggaga atactctcat
     8221 cccccaccag gatcacctcg tcacagactt cttccttgac ggccattacg gcgccttcga
     8281 cctccgggta gggagcttta tcgctgccaa aggcgtcaat ggctactcgc actctactcc
     8341 ttgacggtga ggacttgctt tccgtcgtag gtgccgcact tttcgcagac gtgatgcggg
     8401 cgtttgggct ctccgcattt ggagcaggtg ctcacggcgg gaggagttaa agcgtcatga
     8461 gtgcgacgtt tatcccggcg ggtctttgag gtctttcttt ttgggacggc cattttattc
     8521 tccttgtatt acacatgata tttttctgag cggtgttctc tctgccaggc ctatccaagt
     8581 gtccggacgt tcataagcaa ccgggaatca ggatatgagc tggcaaaact aagcgtttct
     8641 gtgtttaacg tatcaacaac ttacaagacc ccttttttgt caatataaat cacaggagcg
     8701 cttatctgat aagcacttgc atgcttgtcc tggggtggcg tcagcgccgc tgttaccttg
     8761 cgcgtttttt cacatggaga tacagaggct tgctttttca ccacaggaga ccgttgaata
     8821 atcggtatca ctggcttcag agcttgtgtt tcggtctctc ttattagcat tacggtatca
     8881 ttacggactc attacgggtg aagtccgtaa tgagtccgta atgataccgt aatgcgatgc
     8941 ggaaaggtta caggttcagc aggatcagcc ccagggccaa cagcgcgtac atgagcagtt
     9001 ctctgcgaac cggtatccgc ttccagaaca gcgcgtcgct gatcacgctc accaacacga
     9061 tcccactggc cgtgacggga taagctacag cggctggaac ggtggctaaa cctttgagga
     9121 aaaagacggt ggaaagccgg ttggggatgc ccagtccaaa gccaaagagc aacggcagcc
     9181 agcccagctt taccttgcgg atgatgatca atgcggccgt gaagatcagg gcggatgaaa
     9241 acaccatccc gatccagagg gcttcggagc cgctgcccag ttctttgaag agcttgaggc
     9301 tgaggtcggt gagtccggat acggcaaaga gggcgatcag ccacacaaga tggcgccttc
     9361 cctctcctcc cggttttagc ataaatgcca atattgccaa cgcgataccg gcaaagttga
     9421 gcggttttaa gcgctctgca aagatcaata gcgacaccag caccggcagg accatcgcca
     9481 cccgcatcac gctcacggag atggccagac cgttgatcac gatgctgcgc tgatagaccc
     9541 agaaattggt cagaaagagc gctccggccg cgattcccag ccccaggtca aaggcgtgta
     9601 gctgaaatcc tcccctgggg acggccaggt agctaaagac ggttgccatt atgtagttgc
     9661 ccaaaaagac gggcagcatg gcgtggcgcg gatcgggatc agcatggcgc ttgagcttgc
     9721 cgaagagcat caggagattg gcgatgccga cgctgcagag gatgctgagg aggatgttta
     9781 gcatgttagg tgagagctct gctgtgagag ctctgctatg agagctctgc gagctgatga
     9841 gtctgtgggc tgatgcctgg gtgtcttcag cagaaaatcc tgtaaatcat gtcatcgtgc
     9901 aatctgcgtt ctatcattat caattctcaa ttctcaattc tcaattgagc gtcaccttgt
     9961 tttccagcat atcacccggt ttgagatagg caaagctctt gatcctgatc tcatagatgg
    10021 ccatgtccgg atcgtccacg tcgctccagt aatcatagag gaagggggcg tgatcggcca
    10081 cctgtttttt ggtggggagg tcgctgaggg gaatggcgat gccgatgccg cggatgtaac
    10141 cgtttccgga gggatcactt agcggcaggc aaaactcga
//
LOCUS       DAOSFN010000088        10127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5656_length_10127_cov_6.939833, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000088 DAOSFN010000000
VERSION     DAOSFN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10127
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5656_length_10127_cov_6.939833"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLO35_06610"
     CDS             <1..459
                     /locus_tag="PLO35_06610"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOH79374.1"
                     /translation="ATILPLEKTYNPSMSLQVMISNTLLKHGEEFQISVTPNTDGYLY
                     FFDFLSDSSIALVVPNQDLKDNFLKAGQKWEQTVGAIRDPNKEDTIETLYFVFSQDPI
                     AGWEDFKSNRSVDNLVFSAGEESFILFQNWLARSDPKRRVEKMAQIHIFK"
     gene            complement(710..1438)
                     /locus_tag="PLO35_06615"
     CDS             complement(710..1438)
                     /locus_tag="PLO35_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79375.1"
                     /translation="MKAPFPLILVLVLMGLLSGCAVLDVSSLETAIPLQPRRVEISTN
                     QSIGIDLETVVLQDEDLNNGETPPGYRPEANSVTGVQAAIGLQNDMEAGGRFWLSPFN
                     YGGRIFLKKLLDKDVNRYFAIQPAVTYLRSYDDDPGESYIYTAIGSELQLLYTLKASS
                     NFAFTLVGRGNINRYQEQYTDQNGDEIVYGPYTLFQGGLRGNIELRLHPFFFVPELGI
                     EVVPVVNGETTLVPVIGFALGVEL"
     gene            complement(1472..1855)
                     /locus_tag="PLO35_06620"
     CDS             complement(1472..1855)
                     /locus_tag="PLO35_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79376.1"
                     /translation="MRYLPMLRIVLSIILMISCAVLVAQPATKVVNQEDYDLQLVKEK
                     VKNNQPQLTKIYTHESAVYAQGGMLKITLYITEKGKVADVEIKVAAGKFTSTLIKAMK
                     AKILGWKFANKTKLIYTYSLRLSKS"
     gene            complement(1925..3733)
                     /locus_tag="PLO35_06625"
     CDS             complement(1925..3733)
                     /locus_tag="PLO35_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79377.1"
                     /translation="MKLNSFVSAICFSLLISLAGAQSRELEFFFSDGRSARVTENRLA
                     YSIFPGAKSMIGIRAMTGSDERLNFDQKSRRASMEMDLFLTGKLMTHQFLSGYEYLYD
                     ASALEQELSPYQNKTAFLGYGAIFQPLDSLSISFTGKGFIRREQDRYAADRSLKSDGY
                     QALAALRASAGLGETQLGFSAQLDGKSLDWEAYRQSTLNAWMSYGDYVFAIQQNFNWD
                     YREDDLYVLQPDSTRGREGSYSRSDQQKRNSIAYNAFIDYFPSESMRLRFSDVLSRRY
                     ISLKENAVRSNTDLLNQAVLDLDGQILPRLSLNARALYNYGIKDFNYLGNTRRVELRG
                     LSSRLGWEYLDGDSLVATASVELQRTLYPEDQHRWDNDLLTKSLRLGMVHYWKKRIKL
                     SGWMSWNIRDDVYIDGVLSSNNKQVNSIILQPDCSILIGDRLSFKQNYLIRADYTKSM
                     YENPLGEQTRSLYRQLGYRYSLCFDSFPFIARSGDPIWLQLPYRSSHDNAFLVEASFG
                     YEQNDYADYIDTYYSIVSKNIRKSAGLLLKHDIRSLYYIIEPRFSWGTWREYSLLLGL
                     AWRFNNLSLLEVSINPIAEDLDDPDWRSSVNLSLRF"
     gene            complement(3696..4625)
                     /locus_tag="PLO35_06630"
     CDS             complement(3696..4625)
                     /locus_tag="PLO35_06630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HOH79378.1"
                     /translation="MKQLKELGQNFLTDPSVAAEIASAAEIKPGERVWEIGPGRGILT
                     DELLSFDPRLTVFELDRRLIPYLDERYGDRLTLIHGDILRQDWQALISTATTERQSLT
                     LITTELQREQREDFGVGLTGSAIPVPSDSNPARGSSSSTISTSSTSPLKLVSNLPYQI
                     TSPLLELLQRYHDRFDCIVLMLQKEVAQRLQADPGSKAYGALTLRMGLSFDIETLIQV
                     PRYLFEPVPKVDSTVIRLKPRAHPPQICKPEIFQRLIRMGFSSRRKTLRNNLRPLYDS
                     PTLDRLQSQSGIDLSRRAETLSEAEFIRLSDLL"
     gene            complement(4708..4884)
                     /locus_tag="PLO35_06635"
     CDS             complement(4708..4884)
                     /locus_tag="PLO35_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79379.1"
                     /translation="MAKNHRGKGIKTLANHGRGECPSCHRGGVKLLYDIKAGDKTFKV
                     CKVCKAIPADKLGA"
     gene            complement(5022..6080)
                     /locus_tag="PLO35_06640"
     CDS             complement(5022..6080)
                     /locus_tag="PLO35_06640"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOH79380.1"
                     /translation="MEEKHLRFFKDLVSAPSPSGFEAPAQKVARSFMAPYADEIRSDV
                     NGNLIAVKNGSGKLKVMVVGHADEIGFIINHIDEQGYVYVKTLGGFDVNLLPGLRVDI
                     YHDGNLVRGIIGRKPIHMMRGEEGSPKLKLEDLWIDIGASSREDALKRVAIGDIATYN
                     SHFEMLSEDLVVTKATDNKVGVYVAARVMQELAKKKLKANYYAVCSVGEETTMKGAGT
                     SAFQIEPDIAIAVDVTFTSDIPGADKRVFGDVALGKGPTLSIGAALHPVIKDRLIQAA
                     EKLKLPYQIEIAPGRTGTDADAIHALRAGAATAVIGIPNRYMHSPNEVISLSDLDNAV
                     KMTAAFIAALDDKIVLTR"
     gene            complement(6090..6485)
                     /locus_tag="PLO35_06645"
     CDS             complement(6090..6485)
                     /locus_tag="PLO35_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH79381.1"
                     /translation="MKVVDYHDVALEPVNAEGAEGAKIRWLIAQGDGAPNFALRMFEV
                     EPGGHTPHHTHAWEHELYVLAGTGKLVTERGEFAFKADDVMYVDPNMKHSFHNTGEDT
                     LKFLCIIPHEKPVVKKTLNPFAAGTANNC"
     gene            complement(6553..7683)
                     /gene="ribD"
                     /locus_tag="PLO35_06650"
     CDS             complement(6553..7683)
                     /gene="ribD"
                     /locus_tag="PLO35_06650"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOH79382.1"
                     /translation="MSTERQRIHQMMKKAIREAEKARGLCSPNPFVGAVIAKDDRIIA
                     SGHTQAYGSDHAEVQALKKAGKRAKGADLFVTLEPCSHHGKTPPCTDAIIQAGIKRVF
                     FGIRDPNPLVNCDIDTPGPGIISMREAGIQVEWGFLKEQISRQLEYFLCRIQKDRPFV
                     ILKSALSMDGKYAAADGSSRWISNQSSRNYTHKLRQEVDVMLVGISTVLKDDPLLTVR
                     LPRIKRQPLRAVLDACLDIPLDSALIKSIDTAPLLVVHSHASDATIQSKAAMLRGLGV
                     RLACVDAQNELLDLRQALGVLHELGYYSVMLESAGPLAAAFINQQLVDKLIIFYGAKL
                     LGGLHSALADLDIANIDSALELQSTEVRDLDGDLMLSAYPRY"
     gene            complement(7680..8861)
                     /locus_tag="PLO35_06655"
     CDS             complement(7680..8861)
                     /locus_tag="PLO35_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79383.1"
                     /translation="MLSLPKNDRCKICRDEKALRPCPRKRKDIGWRCCNEMRADLHCP
                     DSCAYAPRKDDQEHSPFPAFKADSAGEFTQALKYYIDLWTNIPQTLLEGQSPAALALK
                     DPGRLLDWLSGFRYPSVFPMNYLLEKLHLPRQEETAKEDPETIAARYLDAASVLDWHA
                     LRPMTVNQADDPDLSMRYQEIISAVPILNKIKHHSLIHAGLADDGITALVYVEINRKH
                     DWTLIFSNINGSWQLRQQLNGNPSLFYTQNALHTALAEKLGAGKDAGAWELLERNMFL
                     YPDSADLRYYLALYWQLVKQYDKAKVEYYNSIALDNDFYAPAFSLGSLNLSENRALEA
                     QYWFEYLYARHPDDLNVKNNLAACFAGKGELETAKALWREILNVDPSYELAAKNLERY
                     S"
     gene            complement(8855..9625)
                     /locus_tag="PLO35_06660"
     CDS             complement(8855..9625)
                     /locus_tag="PLO35_06660"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOH79384.1"
                     /translation="MRNIDIDREIERISAFIKGYFSEAGFSKAVIGLSGGIDSAVSAA
                     LTAKALGPENVWGFMLPYKASDPNSLAHALELVTAIGIPHRVIPISTWTDTYFDDYEP
                     GADQLRRGNWMARARMCVLFDQSASLRALVIGTSNRSELLVGYFTQYGDSACALEPIG
                     HLYKTEVKAMAARLRIPTSIIAKAPSADLWSGQTDEAEMGISYKNLDEALFDLTERNI
                     QTWSPALPYSQADFDRASQLMRRSAFKRINPPIPEPPC"
     gene            9857..>10127
                     /locus_tag="PLO35_06665"
     CDS             9857..>10127
                     /locus_tag="PLO35_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HOH79385.1"
                     /translation="MQDKLSKIYEQIKKANECSLGPRAYRDTDAQRIKSENEDEMRTR
                     FAVDRDRILYSGAYRRYHGKTQVFSFTNLIDEEMTNRNLHIAYVSQ"
BASE COUNT         2243 a         2579 c         2893 g         2412 t
ORIGIN      
        1 gccacaatcc tacccctgga aaagacctac aatccctcca tgtctttgca ggtgatgatc
       61 agcaatacgc tgctcaagca cggggaggag tttcagatca gcgtcacccc caacacggac
      121 ggctacctct atttctttga ttttctctcc gacagcagca tcgccctcgt ggtccccaat
      181 caggacctga aagacaactt cctcaaagcc gggcagaaat gggagcaaac cgtgggcgcc
      241 atccgcgatc ccaacaaaga agacacgatc gagaccctct atttcgtctt cagccaggac
      301 cccatcgcgg gctgggaaga tttcaagagc aaccgcagcg tggacaacct ggtcttcagc
      361 gccggggagg aaagcttcat cctcttccag aactggctgg ccaggagcga tcccaagcgc
      421 cgggtggaga agatggcgca gatacatatt tttaagtgag atggctgcgc caggtgagat
      481 ggctgcgcca ggtgagatgg ctgcgccagg tgagatgctt cgcaggtgag atgcttcgca
      541 ggtgagatac tgcgtatgtg agagcgcttc gcgcgggtga ggtctcgggg cttcctctct
      601 tggtgattgg ggccaagctt caaaatacca ggccggaccc acttcggata aatgcgaggt
      661 gggtccggcc tggtctgctt atgataggga ggaggcgata ggggagagct tagagctcca
      721 ctcccagggc gaaaccgatg acggggacca gagtggtttc tccgtttact accggcacca
      781 cctcgattcc cagttccggg acaaaaaaga agggatggag tcggagctcg atattgcccc
      841 tcaggccccc ctggaaaagg gtgtaggggc catagacgat ctcgtcgccg ttttggtccg
      901 tgtactgctc ctgatagcgg ttgatgtttc ctctgcccac cagcgtaaag gcgaagttgg
      961 atgaagcttt gagggtgtag aggagctgaa gctcactgcc gatggcggta tatatataac
     1021 tctcgccggg atcgtcgtca tatgatctga gatatgtgac ggctggctgg atggcaaaat
     1081 agcggttgac gtctttgtcg agcagttttt ttagaaaaat cctgccacca taattgaagg
     1141 gcgataacca gaagcggcct cccgcctcca tatcgttttg gagcccgatg gccgcctgga
     1201 cacctgtgac gctgttggct tcaggccggt agccgggagg cgtctcgcca ttgttcaggt
     1261 cttcatcctg gaggacgacc gtctcgagat cgatcccgat ggattgattg gtgctgattt
     1321 ccactctgcg gggctgaagc gggatagcgg tctcgagaga gctcacatcc agaacggcac
     1381 agccggacag caagcccatc aggaccagga ctaaaatcag aggaaagggc gctttcatag
     1441 atctccttat ctcaatgtaa gcaatgtatc ttcagctctt tgacaggcgt aagctgtatg
     1501 tatagatcag cttggtcttg ttagcaaact tccatcccaa tatcttagct ttcatcgctt
     1561 ttatcaatgt ggaagtgaac ttgcccgcgg ccaccttgat ctccacatcg gcaactttgc
     1621 ctttttcagt gatatagaga gttatcttga gcattccgcc ttgcgcgtaa acggcagatt
     1681 cgtgggtgta aatcttggtg agctggggtt ggttgttttt caccttttct ttgacaagct
     1741 gcaggtcata atcctcctga ttgactactt tcgtggctgg ttgcgccaca aggactgcgc
     1801 agcttatcat gaggatgatg cttaaaacta tccggagcat tggtaaatat ctcatcccga
     1861 cctccatgaa ggacttggcg tatgtagtca tattaacctg tttgacagat ttgccaagca
     1921 ttgcttagaa ccgcaggctg agattgacgc tgctgcgcca gtccggatcg tccaggtctt
     1981 ccgcgatggg attgatcgat acttccagca gggatagatt gttgaagcgc caagccagac
     2041 caagcaacag ggagtattct ctccaagtgc cccaggaaaa gcggggctcg atgatgtagt
     2101 aaagtgagcg gatgtcgtgt ttgagcagca gccccgcgct cttgcggatg tttttggaaa
     2161 cgatggaata gtaggtgtcg atatagtcgg cgtagtcgtt ttgttcatag ccaaagctgg
     2221 cttccaccag aaaggcgttg tcgtggctgg agcggtaggg gagctgcagc cagatgggat
     2281 ccccactgcg ggcgatgaat ggaaagctgt caaaacagag tgagtagcgg tatccaagct
     2341 ggcgatagag gctgcgcgtt tgttctccca gtgggttttc atacatgctt ttagtatagt
     2401 ccgcccggat caggtagttt tgtttgaagc tgaggcgatc gccgatcagg atgctgcaat
     2461 ccggctggag gatgatgctg ttaacctgtt tgttgttgga agaaagcact ccgtcgatat
     2521 agacgtcgtc gcggatattc cagctcatcc agccggaaag cttgatgcgc tttttccaat
     2581 agtggaccat cccgagacgt aaagacttgg tgagaaggtc gttatcccag cgatgctggt
     2641 cctcgggata gagggtgcgc tgcagttcca cggaagcggt cgccaccaga gaatcgccat
     2701 ccagatattc ccatcccagc ctcgaagaca gacctctcag ctccacccgg cgggtattgc
     2761 caagatagtt aaaatccttg atgccatagt tatacaaagc tctggcattc agcgagaggc
     2821 ggggcaggat ttgcccgtcc agatcgagga cggcctggtt cagcagatcg gtgttgctac
     2881 gtacggcgtt ttctttcaag gagatatagc gccgggatag cacatctgaa aagcgcaggc
     2941 gcatggactc ggaagggaag taatcgatga aagcgttgta ggcgatagag ttgcgctttt
     3001 gttgatcgct gcggctgtag ctgccttccc gtccgcgcgt cgaatccggc tgcagcacat
     3061 agagatcgtc ttcacgatag tcccagttga agttttgctg gatggcaaac acatagtcgc
     3121 catagctcat ccaggcgttc agagtggatt gtctgtaggc ctcccaatcc agcgacttgc
     3181 catccaactg agcgctgaac cccagctgcg tttcacccag tccagcggaa gctcttagcg
     3241 ccgcaagtgc ttgatagcca tcgcttttaa ggctccgatc ggcagcgtag cgatcctgct
     3301 ctctgcggat gaaacccttg ccagtgaagc tgatggaaag gctgtccagg ggttgaaata
     3361 tggccccgta gcccaggaaa gcggttttgt tttgataggg agagagctcc tgctccagcg
     3421 cgctggcatc atagagatat tcataacccg ataaaaactg atgggtcata agcttgccgg
     3481 tcagaaagag gtccatttcc atggaagcgc ggcggctttt ttggtcgaag ttgagccgtt
     3541 cgtctgatcc ggtcatggcc ctgatcccga tcatggattt cgcaccggga aagatgctgt
     3601 aggccaggcg gttttctgtg acgcgagcgc tgcggccatc cgagaagaag aattccagtt
     3661 cccggctttg agctcccgcg agagagatca gcaggctaaa gcagatcgct gagacgaatg
     3721 aattcagctt cactgagggt ctccgcgcgg cgggacagat ctatcccgct ctggctttgc
     3781 aggcgatcga gagtggggga gtcatacagg ggacgcaggt tgtttctgag agtcttacgc
     3841 cggctggaaa agcccatccg gatgaggcgt tggaagattt cgggtttaca gatttggggc
     3901 ggatgggcac ggggtttcag tctgatcaca gtggaatcca ccttaggaac cggctcgaaa
     3961 aggtagcgag gcacctggat cagcgtctcg atatcgaagc tgagccccat tctgagcgtg
     4021 agagctccat aagctttgga gcccggatcg gcctgaagcc tttgggccac ttccttttgc
     4081 agcatcagga cgatgcagtc aaagcggtca tgatagcgct gcagcagttc caatagggga
     4141 gaggtgatct gataggggag gttggaaacc agtttgagag gggaagttga tgaggttgag
     4201 atggttgagg agcttgagcc acgcgccgga ttggaatccg acggtacagg aattgcagat
     4261 cccgtcaatc caaccccaaa atcctctctc tgttccctct gtagctctgt agtgattaaa
     4321 gtaagactct gccgctcagt agttgcagta gatatcagcg cctgccaatc ctggcgcagg
     4381 atgtctccat ggatcaaagt cagtctatct ccataccgct cgtctagata ggggatcagc
     4441 ctgcggtcca gctcaaagac ggtgagcctg ggatcaaagc tcaacagctc atcggtgaga
     4501 attccacggc cgggaccgat ctcccagacc cgttctcctg gtttgatctc tgcggcgctg
     4561 gcgatttctg cagccacaga agggtctgtg aggaagttct gtcccagttc cttgagctgt
     4621 ttcacgcggc tataggtggt aaaaaaacga agcccgctat actatctgga aacagatctt
     4681 agcgggcttt tcaagcagga cctgggatca ggcgcccagt ttgtcggcag ggatggcttt
     4741 gcagactttg cagaccttga aggtcttgtc tcccgccttg atatcatata gcagtttaac
     4801 gccgccgcga tggcaggacg ggcattctcc gcgaccgtgg ttggcaaggg ttttgatgcc
     4861 ttttccgcga tgattcttgg ccatgatagc tcctttatgg catgcttatt aggttgttaa
     4921 cagcataacg gtgattttga gccagaaaat tcaagagtgc ccagccgtca acgattattt
     4981 ggcgggaggg cactcctgag ggtgagagtt gaactgtgaa tctagcgcgt cagaacgatc
     5041 ttgtcatcca gagcggcaat gaaggcggcg gtcattttaa cggcgttatc gagatcgctg
     5101 agagagataa cctcattggg ggaatgcata tagcggtttg ggatcccgat cacggcagtg
     5161 gccgcgcccg ctctgagagc gtgaatggcg tcggcgtccg tgccggttct tccgggtgct
     5221 atctctatct gatagggcag tttaagcttt tcggcagcct gaatgagccg gtctttgatc
     5281 actggatgca aggccgcccc gatactgagg gtgggacctt tgccaagggc gacgtctcca
     5341 aacacccgtt tgtcggcccc gggaatatcg gaagtgaagg tcacatccac ggcgatggcg
     5401 atgtccggtt caatctggaa ggcactggtc ccagctcctt tcatggtggt ttcctcacct
     5461 acggagcaga cagcgtagta gttggccttg agcttctttt tggccagttc ctgcatcact
     5521 ctggcggcca catagacgcc cactttattg tccgtggctt tggtgaccac gaggtcttcg
     5581 gaaagcatct cgaaatggct gttgtaggtg gcgatgtctc caatggcgac acgcttgagg
     5641 gcatcttccc tgctggaagc gccgatgtct atccagaggt cttcaagctt gagcttgggg
     5701 ctgccttcct caccgcgcat catgtggatg ggcttgcggc ctatgatgcc gcgcacgaga
     5761 ttgccatcat gatagatgtc cactctgagg ccgggcagga ggttgacgtc gaagccgccc
     5821 agggtcttga catacacata gccctgttcg tcgatatgat tgatgataaa gccgatttcg
     5881 tccgcatggc ccaccaccat tactttcagc ttgccgctgc cgttcttgac ggcgatcaga
     5941 ttgccgttga cgtccgagcg aatctcatcg gcatagggag ccatgaagga gcgggctacc
     6001 ttctgggcgg gagcttcgaa tccggatgga ctgggagcgg aaacgagatc tttgaagaat
     6061 ctgaggtgtt tttcttccat cctgtcctct cagcagttgt tggcagtgcc ggctgcgaag
     6121 gggttgaggg tctttttgac caccggtttc tcgtgcggaa tgatgcacag gaacttcaga
     6181 gtgtcctcac cggtgttgtg aaaagagtgt ttcatattgg gatcgacgta cataacgtca
     6241 tccgctttga aagcaaactc gccgcgttcg gtgaccaatt tgcccgttcc ggcaagcaca
     6301 tagagctcgt gttcccaagc gtgagtgtga tggggagtgt gacctccggg ctccacttca
     6361 aacatgcgca gagcaaagtt gggagcgcca tcgccctgag ctatcagcca gcggatcttg
     6421 gcaccctcag cgccttcggc attgacgggc tcgagcgcaa cgtcgtgata atcgacaact
     6481 ttcatttatt cctccatggt ttcattatga tagatgtgca gatagtataa acgcgcctgc
     6541 ccgttcaggc catcagtatc tgggataggc gctcagcatc aaatcgccgt ccaggtcacg
     6601 gacttcagtg gattgcagtt caagcgcgct gtcgatattg gctatatcca gatcggcaag
     6661 agcgctgtga agaccgccta agagcttggc gccgtagaag atgatcagct tgtcaaccag
     6721 ctgttgattg atgaaagccg cagccagcgg acccgctgat tccagcatca cgctgtaata
     6781 tcccagctcg tgcaagaccc ccagcgcttg tctgaggtca agaagctcgt tttgagcatc
     6841 aacacaggcc agccgcactc ccaaacctct gagcatcgcg gctttactct ggatagtggc
     6901 atcactggca tggctgtgga ccaccagaag gggagcggta tcgatggatt tgatcaaagc
     6961 gctgtccagt ggaatatcca gacaggcatc cagcaccgct ctcagaggct ggcgtttgat
     7021 cctgggcagc cggaccgtga gcagaggatc gtctttcaac acggtagaaa tgcccaccag
     7081 catcacatcc acttcctgcc tgagtttgtg ggtgtaattt cttgagctct gattgcttat
     7141 ccagcgggag gagccatccg cggcggcata ctttccgtcc atgctgagcg cgctttttag
     7201 gatcacaaag ggcctgtctt tctggatgcg gcacagaaaa tattccagtt ggcggctgat
     7261 ttgttccttc agaaatcccc attcgacctg gatacctgct tcccgcatgg atatgatgcc
     7321 gggaccggga gtgtctatat cgcagttgac caatggattg ggatctctga tgccgaaaaa
     7381 gacgcgtttg atccctgcct ggatgatggc atcggtgcag gggggggtct tgccatgatg
     7441 ggagcagggt tccagggtca caaagagatc agcgccttta gcacgtttcc cggctttttt
     7501 gagggcctgc acttccgcgt gatctgatcc gtaggcctga gtatggccac tggcgatgat
     7561 cctgtcatct ttggcgatca cggctcccac gaaaggattg ggagagcaga gtccacgagc
     7621 tttttcggct tcccggatgg cctttttcat catctgatgg atgcgctgcc gctccgtgct
     7681 cacgaataac gctccaggtt cttggcggcc agttcatagc tggggtccac attcaaaatc
     7741 tcacgccaga gtgctttcgc ggtttcgagc tctcccttgc cggcaaagca ggcagccaga
     7801 ttgtttttca cgttgagatc atccggatgc cgggcataca ggtattcgaa ccagtattgt
     7861 gcttccagtg cccggttttc actcaggttc aggcttccca gcgaaaaagc gggagcatag
     7921 aaatcgttgt ccagcgctat agaattgtaa tactccactt tagctttatc atactgcttt
     7981 accagctgcc agtaaagggc cagataatac cgcagatccg cactatcggg atagaggaac
     8041 atgtttcttt ccagcagttc ccaggccccg gcatctttgc cggcacccaa tttctccgcc
     8101 agcgcggtgt ggagagcgtt ctgagtgtaa aagaggctgg ggttgccgtt cagctgttgc
     8161 ctcagttgcc aggagccgtt gatattggag aagatcaggg tccaatcgtg cttgcggttg
     8221 atctccacat acaccaatgc cgtgatgccg tcatcggcca gaccggcatg aatgaggctg
     8281 tgatgcttga tcttattgag gatgggaacg gcggagatga tttcctggta tctcatgctc
     8341 agatcaggat cgtccgcctg attgaccgtc atcggcctta aagcatgcca gtccagaacg
     8401 gaagcggcat ccagatagcg ggccgcgatc gtttccgggt cctctttggc ggtttcttcc
     8461 tgcctgggca ggtggagttt ttccaaaagg taattcatgg gaaacacgct gggataacga
     8521 aatccactca gccagtccag caagcgtcca gggtctttaa gtgccagcgc ggcaggagat
     8581 tgaccttcca gcagggtttg agggatattg gtccaaaggt ctatatagta cttgagcgct
     8641 tgggtaaatt ccccggcgga atcagctttg aaagcaggga aggggctatg ctcctggtca
     8701 tctttgcgcg gagcgtaggc acaggaatcg gggcagtgca gatcggccct catctcgttg
     8761 caacagcgcc agccgatgtc tttacgcttg cgggggcagg gcctgagggc cttttcgtcc
     8821 cggcagatct tgcaacggtc gttttttggc aggctcagca tggcggctcc ggaatggggg
     8881 gattgatccg tttaaaagcg gagcgccgca tcagttggct ggcccggtcg aaatctgcct
     8941 ggctgtaggg aagagcagga gaccaggtct ggatgttccg ctccgtgagg tcaaagagcg
     9001 cctcatcgag gtttttatag ctgatgccca tttccgcttc atcggtctgg ccgctccaga
     9061 gatcggcact gggagctttt gctatgattg aagtcgggat cctgagcctg gcagccatgg
     9121 ccttcacctc agttttgtag aggtgaccga tcggctcgag cgcgcaggcg gaatcgccat
     9181 attgggtgaa gtaacccact agaagctcac tgcggttgct ggtgccaatc accaaagccc
     9241 gcagcgaggc agattgatcg aatagcacgc acatcctggc cctggccatc caattacccc
     9301 ggcgcagctg atctgctcca ggctcatagt catcaaagta agtgtctgtc caggtgctga
     9361 ttgggatcac ccgatgcgga attccaatcg cggtgaccag ttccagagcg tgagccaacg
     9421 aattgggatc gctagcttta tagggcagca taaaacccca aacgttctca ggtccgaggg
     9481 ctttggccgt caaggcggct gaaaccgcag aatctattcc accggagagt ccgatcaccg
     9541 ctttcgaaaa gccggcctcg ctaaagtagc ctttgataaa cgcgctgatc cgctctatct
     9601 ctctatcaat gtcgatattc ctcattttgt aaacctcttt ggggattccc ctcgaacggg
     9661 aaaaccatgt ggtgagcggc gaaccgctac acagaggaca gcctgcgcgc ggactgcttt
     9721 atcgtcaagc ggaatgtgcg catcctaatc ggttttacag gctatgtctg agaatgtatc
     9781 ttgccttggg gcttcagcct ggatatcttg taagtatgtg ataagtacac aaacactcgc
     9841 taacaatgga gatatcatgc aagataaact cagcaagatt tatgaacaga taaaaaaagc
     9901 caacgaatgc agcctggggc cgagagctta ccgggacacc gacgcccaaa gaatcaagag
     9961 cgagaacgaa gacgaaatgc gcacccgctt tgccgtggac agagacagga ttctctacag
    10021 cggagcttac cggcgttatc atggtaaaac ccaagtgttt tcttttacca accttatcga
    10081 tgaggagatg accaaccgca atctccacat cgcctatgta tcccaga
//
LOCUS       DAOSFN010000089         9993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5743_length_9993_cov_8.827128, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000089 DAOSFN010000000
VERSION     DAOSFN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5743_length_9993_cov_8.827128"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2078
                     /locus_tag="PLO35_06670"
     CDS             <1..2078
                     /locus_tag="PLO35_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79386.1"
                     /translation="VYVEFNITNMNIATQDTLFDCAMGIYMDADCGPQSMGSEKASDD
                     LSGYVKGTGYEFAYTHDADGDGGLTTGLVGARVCTPDPEQLRFHCWYWKVGDGPDDGN
                     PYNLSPAPARTANEKYWLLTGRNPNETKFQPLRPEQDDITEYVQPSPNDTRFLFSFYG
                     DMQGLTNPSDASWNLAPGRTMKIVVAVFPGDNKEDLKRTARWAKEIYGDAQTLVNVVL
                     PDTFPHYNPPEPPEIPKMFAEMMDDGSRIDVYWDNRSEFSEDTKTVSSAVIGWQNPAS
                     PAFLPTLDSDPTFIDYSGYPAEFQPNPPNWNMNATVNPYTAYRLRHDFQGYTLWGRSG
                     SGSQEDWVMMDRWDKMETNQDLLDYDVNINSVHADTLYVNYGGYQGIEKGLPNLTTPT
                     SANPWFPPSQFGNYYKYNDQYDLVANPADHFYGRPIYDPAKDYNPAMLADANAIAAAN
                     DGKPAEYIRQLQARLFKHPEMRTDIYDELMDSKLIPLPGHGGQVAIGDADDLEVLKKK
                     RLARRYYQSAIQYPRKGVEYYVAVTAYDRGIPANDLNFLETGRDADANMKVFFPGTIA
                     KSNMDKIYVVPNPYIGRSQFDGRREGDTKGDKSRRLWFVNLPMRCTIRIYTLAGDLVD
                     TIDHDGSYQADVITVSKAATQGIAASGMHDWDLLSKNRQIIAPGVYLYSVENKADNKT
                     KVGKFVIIK"
     gene            2089..3117
                     /locus_tag="PLO35_06675"
     CDS             2089..3117
                     /locus_tag="PLO35_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOH79387.1"
                     /translation="MKKIAILIVALVIMLPMALSAKPFGKTGTAALQFLKLGVDARAI
                     GMGEAYTAVTDDISSTFWNPAGLAPATQNQAFFSHTNWPANIYHEYAAASYTNGVTTF
                     AAYGSVLHMDKIDITDEDFFGPTGEQFTNSSMAFGLNYAQVFTNKFSAGLGIKYLREN
                     LYEFSVNSYAFDVGSMYNTGWKNVKIGMALRNFGPDIRYLVDDDEDGSNDEDPFDLFD
                     NDGDGLIDEDGQELDSKIPMSFSLGISGDIQRTDTTHWIASLQLDNVIDRMETWNLGT
                     EYKMNNLYLRAGYQFNYDTNGLSAGLGYQIASSFAIFNIDYAYTDMGPLAENLFKSAH
                     RLSIKMRY"
     gene            3136..5304
                     /locus_tag="PLO35_06680"
     CDS             3136..5304
                     /locus_tag="PLO35_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79388.1"
                     /translation="MKRLLLVIMTLSLLLSLAYAKEMQAGTNLKPLDTPQGIRMPSTR
                     DVPAYTFTRLPTGIIVNYYDYMIGSYNGLPLRVIPSSAGGGYFMTYHGRRQPTSTRRA
                     FYTYLDAQGNVVSNNEITSVQNHEGYPTVSVDPISGKPLYAWHANADADAEYEVQFVS
                     DAFMAGFAGLWNDIQIVADGPVTITSPSGVTTTTNEFIWPTLQIGPSPVAGKRRAYVA
                     MRNSVTNTAGPSENLYIAYADFNGDDIENGVSLQWSHTSIPEMNQWNVDATWRRPFHA
                     LTTDEAGNLYYAGYHFATEADGTTDIDEEDIDVFICPNYGEGTWTRLDYWSDLATWNP
                     PEAAGSTVGYYTDPDNGDIPYTNDQLSFKLANSSHINASVDQQGRIHVFGVWALSAFN
                     GYYYPALQFVKEFVFDPATQQVNIKEVYPQKPAADNVNSCFTPWDIEAPFGQVDEYGD
                     DGAGGFYPLMVTDWPFPHWDTTAHTEAMLFHYNNTKITDANNEGMMVAVWQNADRARM
                     FNYYSDTDYSAYANTPEIWISVSPNNGALWSEPIVLNNVDTPELAGKKPMWVYPADQV
                     IFTGMQGNQKVGKIGLMFYDDYTWGSNAITPSYHPTPDGGEVMFMELQIVFPYGVDND
                     DPAVTPTQNLLGQNYPNPFNPSTTISFTMPKTTNVNLSIYNVKGQLVNTLVNGSKAFG
                     NHSVVWNGKDVNGNDVPSGIYFYRLSTDDSVETKKMMLMK"
     gene            5547..9713
                     /locus_tag="PLO35_06685"
     CDS             5547..9713
                     /locus_tag="PLO35_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOH79389.1"
                     /translation="MRISMPRFLLLLAFMVMALSANAVVSEYSFTSAAGTYTEITGGT
                     ILGSNLNDDESFNAIPLGFTFTYNGVAYTEISIQTDAFLAFGPTVTNSTLAISSSTGT
                     NNVVAALNRDIMSRDNGELSYLLSGTAPNRVFTIQWKNYKRIPTAAANDVFNFQIQLL
                     EGSNQIKFVYGAFTVVNVTTARTVQVGLRGDSNTDFNNRTTQTDWSATVAGTANNANC
                     WITDTIFPASGLTFTFAPAQQGTPPNPAQTPNPANNGVNVAINANLTWMSGGGTVVGY
                     RIYLGTDNPPTNIVNGTTITNPVYDHPTNFTYNTMYYWKVVPFNTDGDAPDCPVWQFT
                     TLADPTITTYPYEQNFDTLTVPALPLGWSTVNANADAYTWESFAGTAQSTPNSMRIRY
                     NASLAMDDWLITPPMQFTAGTMYLLSFWYRANSATYPEALSVFWGNEPTAAALTNQLF
                     SNSNITNATYQEATATFQPTAAGTYYIGFKGHSPANMFYLYLDSVTISEITDIIDPPN
                     NLTATVNGNDVHLAWEAPGTPPPPPPGFNDGFETYDDFALTFAPWTTVDVDMSTTYGM
                     TGITWPNSYAAQAYMVFNPSTTTPAVTDLTAHSGSKMAACFASTTPPNNDWLISPPIS
                     IGSASTVLSFWAKSYTAQYGLERFKVGISMGGTAPADFTIISGANYIQAPVDWTQYSY
                     PLSSYNGQTVRFGIQCLSNDAFIFLVDDVFVGAPTRENETLFAVNASSPVGRGTRQTG
                     TPVPAVTVPTRDRDLLGYKIYRDNVLIQTINSPTTLVFDDMDLAVGTYSYTVTAYYTG
                     GESTPAGPVTAQIYPPNNPPTDVTATVEGNDVTINWESPETPPTGQWITWCQDVLGNS
                     IGTNAAVTFDVAHRFTQADLAAVAGGTISQVKFVPAHEACVYTVKVWTGGSATNAGTL
                     VSSQVVTAPIIDAWNIAVLNTPVPIPSTGDVYIGFEVNTQGGYPAGCDSGPHIEGKGN
                     MMYFEGAWTTLTALAPTLTYNWLVQTFVSTSTGMKQLELKPIVEAPRPSYPKAELAVY
                     HKDIETRPVTGFKIYRDGVLLTTINDPLATTYTDMDLPNGTYTYGVTALHTGGESVPA
                     TVQVTVNVQLAEIVFQDGFETYENFANLFAPWTLLDVDLSATDGITGIDFPGSAAPMA
                     YIIFNPSATTPPITTLAAHGGAKMAASFAATTPPNNDFLITPRVQLGTNSAIKFYAKS
                     HTAQNGLERFRVGVSLLPTILPQGFQWINGEYTEAPIAWTEYVFDLSAYDNQTVRVAL
                     RCVSNDAFVFYVDDFSLHSNGGIIVDNDDPTIPTIGTRLQGNYPNPFNPETTIRFSVK
                     EATPVTIEIYNTKGQLVKTLINEAKEAGNHSVVWNGKDNSGRSVSSGVYFYKMNAGKY
                     SSTKKMIMMK"
     gene            complement(9786..>9993)
                     /locus_tag="PLO35_06690"
     CDS             complement(9786..>9993)
                     /locus_tag="PLO35_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79390.1"
                     /translation="RELQPRMKMAYKAVSISIKLVKIKFYLGRLGVQSPVRDRDKRII
                     YLGALNSKTAKVLFRAISTAIGSI"
BASE COUNT         2373 a         3143 c         2355 g         2122 t
ORIGIN      
        1 tggtgtatgt ggaattcaac atcaccaaca tgaacatcgc cactcaggac actctctttg
       61 actgcgccat ggggatctat atggatgccg actgcggtcc tcagagcatg ggttctgaaa
      121 aagcatcgga cgacctctcc ggatacgtca agggtaccgg ttatgaattt gcctatactc
      181 acgacgccga tggcgatggc gggttgacca ccggtctggt gggtgcccgc gtttgtaccc
      241 ccgatccaga acagctcaga ttccactgct ggtattggaa ggtgggagac ggtcccgatg
      301 atggcaaccc ctataatctc agccctgccc cagcccgaac ggcaaacgag aagtattggc
      361 tgctcacggg acgcaatccc aacgagacca aattccagcc gctcagaccc gaacaggatg
      421 acatcacgga atacgtgcag ccctcaccca acgatacccg tttcctcttt tccttctacg
      481 gagacatgca aggtttgacc aaccccagcg atgcttcctg gaacctggcc ccgggccgca
      541 ccatgaagat cgtggtggct gtgttccctg gtgacaacaa ggaagacctc aagcgcacgg
      601 cccgttgggc caaagagatc tacggcgacg cccagactct ggtcaacgtg gtgctgcctg
      661 acaccttccc ccactacaac ccacccgaac cacctgaaat ccccaaaatg tttgccgaga
      721 tgatggatga tggcagccgg atcgatgtgt attgggacaa ccgcagtgaa ttcagcgaag
      781 ataccaagac cgtgtccagt gccgtgatcg gatggcagaa tcctgccagt cccgccttcc
      841 tgccaactct ggacagcgat ccaaccttca tcgactatag cggctacccc gccgagttcc
      901 aaccaaatcc tcccaactgg aacatgaacg ccaccgtcaa tccttacacc gcttatcgcc
      961 tccgtcacga cttccaggga tacactctct ggggacgcag tggaagcgga tctcaggaag
     1021 actgggttat gatggaccgc tgggacaaga tggagaccaa tcaggacctt ctcgactatg
     1081 acgtaaacat caacagcgtc catgccgata ccctctatgt gaactatgga ggatatcagg
     1141 gtattgagaa ggggcttccc aatctcacca cgccgacatc ggcaaacccc tggttccctc
     1201 cctctcagtt cggtaattac tacaagtata acgatcagta tgacttggtg gccaacccgg
     1261 ctgaccactt ctatggcagg ccgatctatg atcccgccaa ggattacaat ccagccatgc
     1321 tggcagatgc caacgcgatt gctgctgcca atgacggcaa gcccgcggaa tacatccgtc
     1381 aactgcaggc ccgcctcttt aaacaccctg aaatgcgcac cgatatctat gacgagctga
     1441 tggattccaa gctgatcccg ctgcccggtc atggaggtca ggtggcaatt ggggatgctg
     1501 atgatctcga agttctgaag aagaagcggc tggcccgtcg ctattatcaa tccgccattc
     1561 agtacccccg caaaggcgtt gaatactatg tggccgtcac cgcttacgac cgtggcattc
     1621 ctgccaacga tctgaacttc ctggaaaccg gtcgcgatgc cgatgccaac atgaaggtct
     1681 tcttccctgg caccatagcc aagagcaaca tggacaagat ctacgtggtg cccaatccct
     1741 atattgggcg cagccagttt gacggacgcc gcgaaggcga caccaagggt gacaagagcc
     1801 gccgtctgtg gtttgtaaac ctgcccatgc gctgcaccat ccggatctac actctggccg
     1861 gagacctcgt ggacaccatc gatcacgacg gttcctacca ggctgacgtg atcaccgttt
     1921 cgaaagcggc cacccagggc atcgccgcca gcggcatgca cgattgggac ctgctctcca
     1981 aaaaccgcca gatcatagcc cccggagtat atctctactc cgtggaaaat aaagccgata
     2041 acaagaccaa agtcggcaaa tttgtgatca tcaagtagag gaggaattgt gaaaaaaata
     2101 gctatcctaa tcgtcgcgct ggtaatcatg cttcccatgg ctttatccgc aaaaccgttt
     2161 ggcaagaccg ggacggccgc gctccagttt ctgaagctgg gtgtggatgc ccgtgccatt
     2221 ggaatgggtg aagcctacac cgccgtgacg gacgatattt cctccacttt ctggaatccc
     2281 gccggtctgg cccccgccac gcaaaatcag gcctttttct ctcatacaaa ctggcctgcc
     2341 aatatctacc acgaatacgc cgccgcttct tacaccaacg gtgtcaccac ctttgccgcc
     2401 tacggttcgg tgctgcacat ggacaagatc gacatcacgg acgaggattt cttcggcccc
     2461 accggcgagc aattcaccaa cagcagtatg gcctttgggc tgaactacgc ccaggtcttc
     2521 acaaacaagt tctcagccgg gctcggcatc aagtatctgc gcgagaatct ctatgagttc
     2581 tcggtcaaca gctatgcttt cgacgtcggc tccatgtaca acaccggctg gaaaaacgtc
     2641 aagattggta tggccctgcg caacttcggc cctgatatcc gctatctggt ggacgatgac
     2701 gaagacggca gcaacgatga agaccccttc gatctcttcg ataacgatgg cgacggtctc
     2761 atagatgagg atggacagga gttggacagc aagatcccca tgagcttttc tctgggcatc
     2821 agcggagaca tccagcgcac cgacactact cactggatcg cgtctcttca actcgataat
     2881 gtcatagacc ggatggaaac ctggaacctg ggaaccgaat acaagatgaa caacctctac
     2941 ctgagggctg ggtatcagtt caattatgat accaatggcc tcagtgccgg gttgggttat
     3001 caaatcgcct ccagctttgc catcttcaac attgattacg cttacaccga tatgggcccc
     3061 ctggctgaga atctcttcaa gagtgctcac cggttgtcta tcaaaatgag atactaataa
     3121 ataatggagg aaccaatgaa aagactctta ttggtcatta tgacacttag cctcctgctc
     3181 agtctggctt acgccaaaga gatgcaggca ggcacaaacc tcaagccgct cgatacccct
     3241 caggggatca ggatgcccag cacccgtgac gtaccggctt acacattcac ccggctcccc
     3301 accggcatca ttgtaaatta ttacgactac atgatcggct cctacaacgg cctgcctttg
     3361 agggtgatcc catcctcagc aggtggcgga tacttcatga cctatcatgg tcgccgtcag
     3421 cccaccagca cccgtcgtgc cttctacacc tatttggatg cccagggcaa cgtggtatca
     3481 aacaacgaga tcacctctgt ccagaatcac gagggctatc ccaccgtttc cgtggatccc
     3541 atctccggaa agcccctgta tgcctggcat gccaatgccg atgccgatgc cgagtatgaa
     3601 gtccagtttg tcagcgatgc cttcatggct ggatttgccg gactgtggaa cgacatccag
     3661 atcgtggcgg acggtcctgt caccattacc tcccccagtg gagtgaccac caccaccaat
     3721 gaattcatct ggcccaccct gcagatcggt ccttcaccgg ttgccggaaa gcgccgcgcc
     3781 tatgtggcca tgcgcaattc cgtgaccaac actgccggtc ccagcgagaa cctctatatc
     3841 gcctatgccg atttcaacgg cgacgatatc gaaaacggag tctcattaca gtggagccac
     3901 accagcatcc ctgaaatgaa ccagtggaac gtggatgcca cctggcgtcg tcctttccat
     3961 gccctcacta ccgacgaagc cggaaacctc tattatgcgg gttaccactt tgccaccgaa
     4021 gcagacggca ccactgatat cgatgaagaa gatatcgacg tcttcatctg ccccaactat
     4081 ggcgaaggca cctggacccg gctggattac tggagtgatc tggccacctg gaatcccccc
     4141 gaagccgctg gcagcaccgt gggctattac accgatccag acaacggaga tatcccctac
     4201 accaacgacc agctctcctt caagctggcc aacagctccc acatcaatgc ctcggtagac
     4261 cagcaaggcc gcattcacgt ctttggcgtg tgggcccttt cagcctttaa cggctattac
     4321 tatcccgccc tgcagtttgt gaaagaattc gtgttcgatc cagccactca gcaggtcaat
     4381 atcaaagaag tctatcccca gaagccggct gccgacaatg tgaatagctg cttcacccct
     4441 tgggatatag aagctccctt tggtcaggtg gatgaatacg gtgatgacgg tgccggcgga
     4501 ttttatcccc tcatggtcac agactggccc ttcccccact gggataccac cgctcatacc
     4561 gaagctatgc tcttccacta caacaacacc aagatcaccg atgccaacaa tgaaggcatg
     4621 atggtggccg tgtggcagaa cgccgatcgc gccagaatgt tcaactacta ttccgacacc
     4681 gattacagcg cctatgccaa cacccccgag atctggatct ccgtgagccc caacaatggc
     4741 gcgctctgga gcgaacccat cgtgttgaac aatgtggata ctcctgaact ggccgggaag
     4801 aaacccatgt gggtctatcc tgccgatcag gtgatcttca ccggcatgca gggcaaccag
     4861 aaggtcggaa agatcggtct catgttctac gacgactaca cctggggctc aaacgccatt
     4921 actccttcct atcacccgac tccggacggt ggcgaagtga tgtttatgga actacagatt
     4981 gtcttcccct atggcgtgga caacgatgat cctgccgtga ctcccaccca gaatctgctg
     5041 ggccagaact accccaatcc cttcaatccc agcaccacca tcagcttcac catgcccaag
     5101 accaccaacg tcaacctttc catctacaac gtgaaaggcc agttggtcaa taccctggtc
     5161 aacggcagca aggcttttgg caaccacagc gtggtctgga atggaaaaga tgtcaatggc
     5221 aacgacgtgc ccagcggaat ctacttctat cgtctgagca ccgacgacag tgtcgaaacc
     5281 aagaaaatga tgctgatgaa ataatcctgg gtcagcagac caatatacat agcgccggag
     5341 tgatccggcg cttttattat atggtattga agctgggctc gcggggccgg gttgtgtgca
     5401 aaagaacacc ctaagcggga ataagttttg cttgacacat agagccactt ggtttgtctg
     5461 gcagactgaa ggggattata ccgccagcgg tttgaaaacc tctcagttta aagcaaatag
     5521 atattaactt aagacaagga gaacttatgc gcatcagtat gccgagattc ctgctcctgt
     5581 tggcctttat ggtaatggca ctttctgcca acgctgttgt gagcgaatat tcgttcacca
     5641 gtgcggcggg tacctacacc gagatcacag gaggcactat cctgggtagc aacctgaatg
     5701 acgatgaaag cttcaacgcc attcccctgg gattcacctt cacttacaac ggtgtagcct
     5761 acaccgagat cagcattcag accgatgcct ttttggcctt cggtcccact gttacaaaca
     5821 gcacccttgc catcagcagc tcaaccggca ccaacaacgt cgtggctgcc ctaaacagag
     5881 acattatgag ccgtgataat ggcgagcttt catacctgct tagcggcacc gcccccaatc
     5941 gcgtcttcac gatccagtgg aaaaactata aacgtattcc caccgcggca gctaacgatg
     6001 tctttaattt ccagattcaa ctgctggaag gcagcaacca gatcaagttc gtttatggcg
     6061 cctttactgt cgtaaacgtc accacagccc gcaccgttca ggtgggcctg aggggtgata
     6121 gcaacaccga cttcaacaac cgcaccaccc agaccgattg gtctgccaca gtggccggaa
     6181 ccgccaacaa tgccaactgc tggattaccg ataccatttt cccggccagc gggcttacct
     6241 tcaccttcgc tcctgcccag cagggtactc ctcccaatcc agcccagacc cccaaccccg
     6301 ccaacaatgg cgtcaacgtg gccatcaatg ccaacctgac ctggatgagc ggtggcggta
     6361 ctgtggttgg atacaggatc tatctgggaa cggacaatcc acccaccaac atcgtcaatg
     6421 gtactaccat caccaatccc gtctatgacc accccaccaa ttttacctac aacacgatgt
     6481 actactggaa agtagtgcca ttcaacaccg atggcgatgc tcccgattgc ccggtctggc
     6541 agtttaccac tctggcagat cccaccatca ccacctatcc ctacgagcaa aactttgata
     6601 cactgaccgt ccccgcttta cctctgggat ggagcaccgt caacgcgaac gccgatgcct
     6661 acacttggga aagctttgcc ggaacggccc agagcactcc caattccatg cggattcgct
     6721 acaatgcctc gcttgccatg gatgactggc tgatcactcc ccccatgcag tttaccgccg
     6781 gcacgatgta cctgctgagc ttctggtaca gggccaacag cgccacctat ccggaagccc
     6841 tctcggtctt ctggggcaac gagcccactg ccgcagccct caccaatcag ctgttcagca
     6901 acagcaacat taccaatgcc acctatcagg aggccactgc cactttccag cctaccgctg
     6961 ccgggaccta ctacattggc ttcaagggcc acagccccgc caatatgttc tacctctatc
     7021 tggatagtgt caccatttcc gaaatcacgg acatcatcga tcctcccaac aacctcaccg
     7081 ccaccgtgaa cggaaatgac gtgcacctgg cctgggaagc tcccggaacc cctcctcctc
     7141 cccctcccgg attcaatgat ggctttgaga cctatgacga tttcgccctc actttcgccc
     7201 cctggacgac tgtggacgta gacatgagca ccacctacgg catgaccggg atcacctggc
     7261 caaactctta tgccgcacaa gcttatatgg tattcaaccc cagcaccact actcccgccg
     7321 tcaccgatct caccgctcac agcggatcca agatggccgc ctgctttgcc agcaccactc
     7381 ctcccaacaa cgactggctg atcagccctc ccatctcaat cggcagtgcc agcaccgtcc
     7441 tcagcttctg ggccaagagc tacaccgctc agtatggttt ggaacgcttc aaagtcggta
     7501 tctccatggg cggaaccgct ccggctgact tcactatcat cagtggcgcc aactatatcc
     7561 aggcccccgt ggactggacc cagtacagct atcccctcag cagctacaac ggtcagaccg
     7621 tccgtttcgg tatccagtgc ctgtccaacg acgccttcat cttcctggtg gacgatgtct
     7681 tcgtcggtgc gcccacccgc gagaacgaga ccttgtttgc cgtcaatgcc agctcccctg
     7741 tgggacgcgg cacccgtcag accggcactc ccgttcctgc cgtcaccgtt cccaccagag
     7801 accgtgacct gctgggctac aagatctatc gtgacaacgt gttgatccag accatcaaca
     7861 gccccaccac ccttgtcttt gacgatatgg acctggctgt gggcacctac agctacaccg
     7921 tcaccgctta ctataccggt ggagaatcaa ctcccgccgg ccccgtgacc gctcagatct
     7981 acccgcccaa caatcctccc accgatgtta ccgccactgt ggaaggcaac gatgtgacca
     8041 tcaactggga gagtcccgaa accccgccta ccggtcagtg gatcacctgg tgccaggatg
     8101 tgcttggcaa ctccatcggc accaatgccg ccgtgacctt tgatgtggcc catcgcttca
     8161 cccaggctga ccttgccgcc gtggccggtg gcaccatcag ccaggtcaag tttgtgcccg
     8221 ctcatgaagc ctgcgtctat accgtgaaag tatggaccgg cggctcagcc accaatgccg
     8281 gtaccctggt atcctctcag gtagtcaccg ctcccatcat cgatgcctgg aacatcgcgg
     8341 tgctcaatac cccagtcccg attcccagca ccggcgacgt ctacatcggc ttcgaagtaa
     8401 acactcaggg cggctatcct gccggttgcg acagcggtcc ccatattgaa ggcaaaggca
     8461 acatgatgta tttcgaggga gcctggacca ctctgaccgc tctggccccc acccttacct
     8521 acaactggct ggtccagacc tttgtgagca ccagcactgg tatgaagcag ctggagctca
     8581 agcccatcgt agaagctccg cgtcccagct atcccaaagc cgaactggcc gtctatcaca
     8641 aggatatcga gacccgtccc gtgactggat tcaagatcta tcgtgatggc gtcctgctta
     8701 ccacgatcaa cgatccgctg gccaccacct ataccgatat ggaccttccc aatggcacct
     8761 acacctatgg cgtgaccgcc ctccacaccg gtggggaatc agttcccgcc accgtgcagg
     8821 tgaccgtcaa cgtccaactg gccgagatcg tgttccagga tggctttgag acctacgaaa
     8881 acttcgccaa cctctttgcc ccctggaccc tgctcgatgt cgatctttcc gctaccgatg
     8941 gaattaccgg gatcgacttc ccaggttcag ccgctccgat ggcctatatc atcttcaatc
     9001 ccagcgctac gacgcctccg atcaccactc tcgctgctca cggtggggcc aagatggctg
     9061 ccagttttgc cgccaccacc cctcccaaca acgacttcct gatcactccg cgcgtccagc
     9121 ttggcaccaa cagcgccatc aaattctatg ccaaatccca caccgctcag aatggactgg
     9181 agagattccg cgtgggcgtg tctctgcttc ccactattct gccgcaggga ttccagtgga
     9241 tcaacggtga atataccgaa gctcccatcg cctggactga gtatgtgttt gacctcagcg
     9301 cctatgacaa tcagaccgtc cgcgtggccc tccgctgcgt ctccaacgat gccttcgtgt
     9361 tttacgttga tgatttcagc ctgcacagca atggcggtat aatcgtggac aacgacgatc
     9421 ctaccattcc caccatcggt accagattgc agggcaacta tcccaacccc ttcaatcctg
     9481 aaaccaccat ccgcttcagc gtcaaggaag ccactcccgt caccatcgag atctacaaca
     9541 ccaaaggtca gctggtgaag accctcatca acgaggccaa ggaagccggc aaccacagtg
     9601 tggtttggaa tggcaaagac aactccggtc gctccgtatc cagcggcgtc tatttctaca
     9661 agatgaacgc cggcaaatac agcagcacca agaaaatgat catgatgaag taaagccgct
     9721 tcgtcagaga taacatcgcg ccctggtttc gaccggggcg cttttctttt ggggggtctt
     9781 ttttttcaga tggaacctat ggctgtcgag attgcgcgaa acagcacctt ggccgtcttg
     9841 gagttcaaag cccctaaata gattatgcgt ttatctctat ccctgacggg gctttggact
     9901 ccaagacggc caaggtagaa ttttattttc acaagcttta tagatattga tacggcttta
     9961 tatgccatct tcatacgagg ctgtagctcc ctt
//
LOCUS       DAOSFN010000090         9948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5787_length_9948_cov_9.070555, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000090 DAOSFN010000000
VERSION     DAOSFN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5787_length_9948_cov_9.070555"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1535
                     /gene="tilS"
                     /locus_tag="PLO35_06695"
     CDS             66..1535
                     /gene="tilS"
                     /locus_tag="PLO35_06695"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOH79391.1"
                     /translation="MRALEKGLDKLHKYIVDKSLFASGSKLLLAVSGGSDSVGLLCLF
                     ARLRSRLPITLLCCHVNHGLRGEQSDADEALVKDLCLRLNVPLIARKIKLEAGADLEN
                     RARKLRFEALFHLLELYRFDYVVLGHQREDQAETALMNMLRGAGINGLAGIKVKQGKV
                     LHPLLDFGRAELRDMLIEEGLKWREDASNQDPRFRRNRLRLELLPHLEERYSADIVDK
                     LNRQSQILSDADAYFRGLAQTHLKRLSLESDSGHLSLSVKGISKLQRVELYYLLKLAY
                     TSVSGEHKDFFYHNFEDLLVLLKGQGSSMINLPNGVIAQREYDILSMRMGQAEIPVPE
                     ELSVDEDRARVVYGAYRFSFRHLKLLPREQALDHFSVVLDADKLIWPLCLRSRRDGDR
                     FVPQGMSHSKKLKDFFIDAKVPKSSRDQVPILEDGEKLLWVVGLRADARALADHNSTR
                     LLQIVAEPTVEKPKRAASRVKTQGGKSEPDELRYFSSPF"
     gene            1495..2040
                     /gene="hpt"
                     /locus_tag="PLO35_06700"
     CDS             1495..2040
                     /gene="hpt"
                     /locus_tag="PLO35_06700"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOH79392.1"
                     /translation="MNQMNCDISAVLFDEYKIQTRIREIGSQISMDYNGKVPVLIGIL
                     KGGFVFLADLARAISIPMEVDFLAISSYGSGTTSTGAVKIRKDIDINIEGRDVIIVED
                     IVDSGLSLQYIKDYIWKHKPSSVRVCVLLDKPQAHKLEVSFDYVGFEVGNEFVVGYGL
                     DFDEHYRNLPYIGILKEEMYS"
     gene            2037..4346
                     /gene="ftsH"
                     /locus_tag="PLO35_06705"
     CDS             2037..4346
                     /gene="ftsH"
                     /locus_tag="PLO35_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOH79393.1"
                     /translation="MKRLFVALLCLWAWMAVFAQADSLASGTQAARDSVPRVESAPVP
                     QQPVPELPPEEVQRTFTEFGVMMRWFVYALIAISVISLIRIWLVKRRGRDGSETPPPV
                     FPLDDKEKKPGEQPQERRPMPPVMKRSPVSFTFILVLLALLFVFFSTWNSSRQKIENK
                     SYTEFNAQLKLGQIARAEFTERDILYTDTNKVKYHTIMPFENPALVDSLVERGVMVIT
                     NRPSRWGRLLEYMLPLLLLVAFWLFFLRGMNNQNSKAFSFGKSKARLHEASKSGITFK
                     DVAGVDEAKEELQEIVEFLKDPKKFQRLGGRIPRGVLLIGRPGTGKTLLAKAVSGEAG
                     VPFFSISGSDFVEMFVGVGAARVRDLFEQAKKNSPCITFIDEIDAVGRHRGTGLGGGH
                     DEREQTLNQLLVEMDGFEPNEAVIIIAATNRPDILDPALLRPGRFDRQVTVDLPDIKG
                     RTEILKVHAAKVPLGTDVNLELIARGTPGFSGADLANIVNEAALIAASANKKEIGMID
                     FEEAKDKLILGKEKRSRVIPEDDKRLTAYHEIGHVLASVFQDKTEPVHKVSIIPRGFS
                     AGATHFLQTDKMGYSRGYLEQLMVELLGGRAAEEEVFGELTTGAGNDLERVTDIAKKM
                     VCTWGMSDRIGPMTVGKDQGEVYLGKELVSRDIHSNETARMVDEEIRGFVATAHKRAL
                     DIMTEHRDLLDKMAIELFEKETLGTDEIFEMILADVDVAKQAMIEAKYAKAKELRFEH
                     SEVLDAETIASSVPEPAQPSQQSSEGLED"
     assembly_gap    4568..4667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4696..5229
                     /gene="hslV"
                     /locus_tag="PLO35_06710"
     CDS             4696..5229
                     /gene="hslV"
                     /locus_tag="PLO35_06710"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOH79394.1"
                     /translation="MSLWHGTTIIGIHRNGHTAICGDGQVTMGDAVVKSKAIKIRRIF
                     DGKVIIGFAGATADAFTLFEKFEEKLKAHKGALRKAAVELARDWRSDRYLRRLEAMLI
                     AGDATGIIVISGVGDVLEPDDGIAAIGSGGNYALAAARAMALHSKLDNRTIVTEAVRL
                     ASEICVYTNANLVVEEI"
     gene            5226..6575
                     /gene="hslU"
                     /locus_tag="PLO35_06715"
     CDS             5226..6575
                     /gene="hslU"
                     /locus_tag="PLO35_06715"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOH79395.1"
                     /translation="MTFAPDELTPQRVVEELDKYIVGQHAAKRSVAIALRNRWRRQQI
                     DSDLQQEIMPNNIILIGPTGVGKTEIARRLSRLANAPFIKVEASKFTEVGYVGRDVES
                     MVRELMSNAVNQIRHQMLENVQLEAYDAALNRVLDLLVPVANSEKDPETAARKARTRE
                     KMLDKLISGKLDDREVEFKAEDIPSATLELLNPQSMDMAEFDISEMISGLFPGQNKKK
                     RLRMSVKEAIDYFMEKEANRMVSKEKVVEAAREAVENDGIIFIDEIDKIASPDRHGQV
                     DVSRSGVQRDLLPIVEGSVVPTKYGPIDTCHVLFIAAGAFHTAKPSDLIPELQGRFPI
                     RVELNSLTQADFERILVEPENALIKQYQALFAADGCKLSFSKDAVAEIARYAALANEK
                     MEDIGARRLHTIMNTLLDEYLFDMPNPTLTSLKVTPKLVRAKMARVIESDDLARFIL"
     gene            6575..7879
                     /locus_tag="PLO35_06720"
     CDS             6575..7879
                     /locus_tag="PLO35_06720"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH79396.1"
                     /translation="MKKIKELFVALTVRNYRLFFMGQGISLIGTWMQRTTMGWFVYRL
                     TNSAFLLGVISFLSMIPALFISPFAGAWGDRWNRHHTLIITQSLFMLQAALLALAAIS
                     GFINADRIWPLMALSLMQGVIEAVDSPIRQSFVIDLVAKRSLLPNAIATNSAMFNSAR
                     LVGPSFGGAMILLFGEGMCFAINAISYIPVIVMLFMIHLSYPARKPQSESTFTKIKEG
                     WNYAFGHLPIRFLISNLMVFTLFGMSYSTLIPVFARDILKGTAGTQGLLLSTAGIGAL
                     CTSFYLASRKTIKGLPTVMISLGVAVSLVLIIFSRSSSLPLSLFLMLFIGLGMTMQMA
                     STNTIIQSVVADHMRGRVLSVYTMSFMAASPFGGLLAGTLTRQFGPRNALSICAGVCL
                     IWALFGLRLIPNIVRDIFRMLIISGNKEIYRPVAVKLNPGEV"
     gene            7876..8565
                     /locus_tag="PLO35_06725"
     CDS             7876..8565
                     /locus_tag="PLO35_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOH79397.1"
                     /translation="MKTWLHLGNGTPDYRSIMAAHHDFYIGDKLIPASRVVICQQTHS
                     REVHICSEADSGAGFGAHPQIAVADGFVTNVPHQYLLIRTADCTPVLFTDKLHRAVGA
                     VHSGREGTRKNIVSRAVELLKSEYGIEPRDLCAWIGAGICVDHYQVNRAVYDAFHTSL
                     IETGISPGPRDDLKLDIRHCIFQQLIAAGIAFRDIEQDHSCTFETQGYHSFRRDGTHN
                     RQINLVGIEYD"
     gene            8558..9838
                     /locus_tag="PLO35_06730"
     CDS             8558..9838
                     /locus_tag="PLO35_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOH79398.1"
                     /translation="MTDTYTNPSGNLSLRISEDKLSAWLTVHTSGKLINERDILDLID
                     RAGIKYGFEDALRYIRAHNLEKDFDQPFPVAMCKIGGSGNTIKRFFDVEQARAFNPRV
                     TPEQIPSLTCVEAGTVLADLSDNIFDREGSIYNVLGEMIAPTQTDLDAATAMAGENVS
                     YLPEKGQFVSVKSGYIALDDEDRVSVHSTLIIQGDLEGVQDLRAPVDLIVEGSIRGSG
                     IHLQGGLSVSGDIYESGISCGGDLLVGGEIRSCRQGGLEVIGDLRCGRIISSRVLCRG
                     LLSCSEIRSSEVVGEKGISTQADGPIIASNAQSAVRIEAGHAGEADDESETVLEITIS
                     PFLKALMMHLTRELVHAKQNGRQEEVKEIAARISTCEHELDRELNDFLVCESDQLRGV
                     KIIGNVYPVLKVRVLKHEYKISKYQSGLSIDEKQ"
BASE COUNT         2287 a         2579 c         2908 g         2074 t
ORIGIN      
        1 ccccttgaca atcctccccc ctccaaaaag cttactttct ctaaaaatat cacaatggag
       61 cagcgatgcg cgccctggaa aaaggcctgg acaaactgca caagtacata gtggacaaga
      121 gcttgtttgc aagtggaagc aagctgctgc tggccgtgtc cggtggatcg gactcggtgg
      181 ggctgctgtg tctgttcgcg cggctgaggt ccaggctgcc catcacgctg ctctgctgtc
      241 atgtgaatca cgggctcagg ggtgagcaaa gcgatgccga tgaggcattg gtgaaagatc
      301 tgtgcctgcg gctgaacgtt ccgctgatcg cgcgtaagat caagctggaa gctggagcgg
      361 acctggaaaa ccgggcccgc aagctgaggt ttgaagcgct gtttcacctg ctggagctct
      421 atcgcttcga ttacgtggtg ctgggccatc agcgtgaaga ccaggctgag acggccctga
      481 tgaacatgct gagaggggcc ggcatcaacg gtctggcggg gatcaaagtc aagcagggca
      541 aggtcttgca tcctctgctg gattttggca gagcggagct gagagatatg cttattgaag
      601 aggggctgaa gtggagagaa gacgccagca accaggatcc caggttcaga cgcaaccgtc
      661 tgcggctgga gctcttgcct catctggaag agcgttacag cgcggacata gtggacaagc
      721 tcaaccgcca gagccagatt ttgagcgatg ccgacgcata ctttcgcggt ctggcccaga
      781 cgcatctgaa gcggctgagc ctggagagcg attccggtca tctcagtctg agtgtcaaag
      841 ggatcagcaa gctgcagcgc gtggagctct attatctgct caagctggcc tacacaagtg
      901 tcagcggcga gcataaagac ttcttttatc ataactttga ggacctcctg gtgctcttga
      961 aaggccaggg cagcagcatg atcaatctgc ccaacggtgt gatcgctcag cgggagtatg
     1021 acatcctcag tatgaggatg ggccaggctg aaatccctgt tccggaggag ctgagcgtgg
     1081 atgaagaccg cgcccgggtg gtctatggcg cttatcgctt tagtttcaga cacctcaagc
     1141 tgctgccgcg cgagcaggcg ctcgaccatt tcagcgtggt attggatgcc gacaaactga
     1201 tctggccgct ctgcctgagg tccaggagag acggcgaccg ttttgtgcct cagggcatga
     1261 gccacagcaa aaaactaaaa gactttttta tcgatgccaa ggtgccaaaa agctcccgcg
     1321 accaggtgcc catcctcgag gacggagaaa agctcctctg ggtggtgggc ttgagagcgg
     1381 acgcgcgtgc tttggcagat cataacagta cacggctctt acagatagtg gctgagccca
     1441 cggtggaaaa acccaaacgg gccgccagcc gcgtcaaaac tcagggagga aagagtgaac
     1501 cagatgaatt gcgatatttc agcagtcctt tttgacgaat ataagattca gacccgcatc
     1561 cgcgagatcg gcagtcagat ctccatggac tacaacggca aggtgcccgt cctgatcggc
     1621 atcctcaagg gtggatttgt gtttctggcc gatctggcga gggcgatcag cattcccatg
     1681 gaggtggact ttctggcgat ctccagttat ggcagcggca ccaccagcac cggtgccgtc
     1741 aagatccgca aggatataga catcaatatc gaaggtcggg atgtgattat cgtggaagat
     1801 atcgtggata gcggtctttc gctgcagtat atcaaagact atatctggaa acacaaaccc
     1861 agcagcgtgc gcgtctgcgt gctgttggac aaacctcagg cccacaagct ggaagtgagc
     1921 tttgattatg tgggcttcga ggtgggcaac gagtttgtgg tgggctacgg tctggatttt
     1981 gacgagcact atcgcaacct gccttatatc ggcatcctca aggaagagat gtattcatga
     2041 aaagactttt tgtggccttg ctctgcctat gggcctggat ggcagttttt gcccaggcgg
     2101 acagccttgc gtcaggaacc caggctgccc gggacagcgt tcccagggtg gagtcggcac
     2161 cagtgccgca acagcctgtg ccagagcttc ctcctgaaga agtgcagcgt acctttaccg
     2221 agtttggggt gatgatgcgg tggtttgtct atgccctgat cgcgatcagc gtgatcagcc
     2281 tgatcaggat ctggctggtg aagagacgcg gtcgcgatgg ttccgaaacc ccaccccccg
     2341 tctttcctct ggatgacaaa gagaaaaagc ctggcgaaca gcctcaggag cgcaggccta
     2401 tgccacctgt gatgaaaaga tcaccggttt ccttcacctt tatcctggtg ctgctggccc
     2461 tgctctttgt gtttttcagc acctggaaca gctcccggca aaagattgaa aacaaaagct
     2521 ataccgagtt taacgcgcaa ctgaagctgg ggcagattgc cagagcggag tttaccgaac
     2581 gcgacatcct gtataccgat accaacaaag tgaagtatca caccattatg cctttcgaga
     2641 atcccgccct ggtggacagc ctggtggagc ggggcgtgat ggtgatcacc aaccgtcctt
     2701 cccgctgggg caggctgctg gaatatatgt tgccgctttt gctgctggtg gcgttctggc
     2761 tctttttcct gagagggatg aacaaccaga attccaaggc tttcagcttt ggcaagagca
     2821 aggcgcggct ccacgaagcc agcaagagtg gcatcacctt caaggacgtg gcgggggtcg
     2881 atgaagccaa agaagagctg caggagatcg tggagtttct caaggacccc aagaagtttc
     2941 aaaggctggg cggcaggata cccagagggg tactgctgat cggaagaccg ggaacgggta
     3001 agaccctgtt ggccaaagcg gtttccggag aggcgggcgt gccgtttttc agcatcagcg
     3061 gctcagactt tgtggagatg tttgtggggg tgggggcagc cagagtgaga gacctctttg
     3121 aacaggccaa gaaaaacagc ccctgcatca cttttatcga cgagatcgat gccgtgggcc
     3181 gtcatcgcgg aaccggattg ggaggaggtc atgacgagcg tgagcagacc ctcaatcagc
     3241 tgctggtgga gatggacggc tttgagccca acgaagcggt gatcatcatc gccgctacca
     3301 accgtcccga catcctggac cccgcgctcc tgagaccggg acgttttgac cgtcaggtga
     3361 cggtcgatct gccagatatc aaaggccgca ccgagatcct caaggtccat gccgccaagg
     3421 ttccactggg aactgatgtc aatctggaac tgattgccag aggcacccct ggattcagtg
     3481 gcgccgatct ggccaatatc gtcaatgaag ctgccctgat agccgcctcc gccaacaaga
     3541 aagagattgg tatgatcgat tttgaggagg ccaaggacaa gctcatcctc ggcaaggaga
     3601 aacgcagccg cgtgattccc gaagacgata aacgcctcac tgcctatcac gagatcgggc
     3661 atgtgctggc aagcgtgttt caggacaaga ccgagccggt gcacaaggta tctatcatcc
     3721 ccagaggctt ttccgccggg gccacgcatt ttctgcagac cgacaagatg ggctattcca
     3781 gaggctatct ggaacagctg atggtggaac tgctgggtgg acgggccgcg gaagaggaag
     3841 tgtttggaga gcttactacc ggagcgggaa acgatctgga gcgggtgacc gatatcgcca
     3901 agaagatggt ctgcacctgg ggcatgagcg atcgcatcgg tcccatgacc gtgggcaaag
     3961 atcagggcga ggtgtatctg ggcaaggaac tggtatccag agatatccac agcaatgaaa
     4021 cggcccgcat ggtggatgag gagatcagag gctttgtggc caccgctcac aaacgcgctc
     4081 tggacatcat gaccgagcac cgggacctcc tggataaaat ggccatagag ctgtttgaaa
     4141 aggagaccct gggcacggac gagatctttg agatgatcct ggcagatgtg gatgtggcca
     4201 agcaagctat gatcgaagcc aaatatgcca aggccaaaga gctgcgcttt gaacacagcg
     4261 aggtgctgga tgctgaaacg atagcttcca gcgttccgga acctgcgcaa ccctcccaac
     4321 agagctcaga aggtttagag gattgatcag gtttagaagt caggcaggtt tcccacagat
     4381 ttcgcggatt ggcgcgcaga tctcgcggat tgttataccg tttagagatt tgagaagtac
     4441 cagcgctcgc tgtggtgcca ctctctcaac cttctcaaca gatcctaaag ggattagacc
     4501 tctgcccatt gaacccgaaa aaattctgcg aaatctgcgc accaatctgc gtaatctgcg
     4561 ggaaatgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngcc tacctttcca
     4681 aaaggagaag aaagtatgag cctctggcat ggaaccacca tcatcggcat ccacagaaac
     4741 ggccataccg ccatctgtgg agacgggcag gtcacaatgg gcgacgccgt cgtcaagagc
     4801 aaggcgatca agatccgtcg catcttcgat ggcaaagtga tcatcggttt tgccggagcg
     4861 acggcagacg cttttaccct gtttgaaaag tttgaagaga agctcaaggc ccacaaagga
     4921 gccttgcgca aagccgctgt cgagctggca agagactggc gcagcgatcg ttatctgcgc
     4981 aggctggaag ccatgctgat cgctggggat gccactggca ttattgtgat cagtggagtg
     5041 ggggacgtgc tggaacccga cgatggcatc gccgccatcg gttccggtgg caactacgct
     5101 ctggcagctg ccagggcgat ggcactgcac agcaagctgg acaaccggac cattgtgacg
     5161 gaagcggtgc gcctggcctc tgagatctgt gtgtatacaa acgccaatct ggtggtggaa
     5221 gagatatgac attcgctccc gatgagctca cccctcaaag agtggtggag gaacttgaca
     5281 agtatatcgt aggccagcat gctgccaagc gcagcgtggc catcgcgctc agaaaccgct
     5341 ggcgccgtca gcagattgac agtgatctcc agcaggagat catgcccaac aacatcatcc
     5401 tgatcggtcc taccggagtg gggaagactg agatcgccag aaggctgtcc aggctggcca
     5461 acgctccttt catcaaggtg gaagcctcca agtttaccga ggtgggatat gtgggcaggg
     5521 atgtggaatc gatggtgaga gagctgatga gcaacgccgt caatcagatc cgtcatcaga
     5581 tgctggagaa tgtgcaactg gaggcctacg acgcggcttt gaaccgggtg ctcgatctgc
     5641 tggtgccagt ggcaaacagt gaaaaagatc ccgagaccgc ggccagaaaa gcccgtaccc
     5701 gtgaaaagat gctggacaag ctgatcagcg gcaagctgga cgaccgggaa gtggagttta
     5761 aagcggagga catacccagc gccaccttgg agctcctgaa tcctcaaagc atggatatgg
     5821 cggagtttga tatcagcgag atgatctctg gcctctttcc cggccagaac aaaaagaagc
     5881 gcctgcgcat gagcgtgaag gaagccatcg actactttat ggaaaaagag gccaaccgca
     5941 tggtgagcaa ggagaaggtg gtggaagccg ctcgcgaagc ggtggaaaac gatggcatca
     6001 tcttcatcga tgagatcgat aagatcgcct ctccggaccg ccacggtcag gtggacgttt
     6061 cccgcagcgg agtgcagcgc gatttgctgc ccatcgtgga aggaagcgtg gtgcccacca
     6121 aatacggtcc catcgacacc tgccatgtgc tattcatcgc ggcgggggct tttcacaccg
     6181 ccaagccaag tgatctgatc ccggagctgc aaggccgctt tcccatccgg gtggaactca
     6241 acagcctcac gcaagcggat tttgagcgca ttctggtgga gccggaaaat gctctcatca
     6301 agcagtatca agctctcttc gccgcggatg gctgcaagct cagcttcagc aaggacgcgg
     6361 tggctgagat cgccagatat gccgctctgg ccaatgaaaa gatggaagac atcggggccc
     6421 ggcgtctgca tacgatcatg aatacgctct tggacgagta cctgtttgac atgcccaatc
     6481 ccacgctgac cagcctgaaa gtgactccga agctggtacg tgccaagatg gccagagtga
     6541 tcgagagtga cgatctggcc cgctttatcc tctgatgaag aagatcaaag agctgtttgt
     6601 agccctcacg gtgcgcaact accgtctctt tttcatgggt caggggattt ctctgatcgg
     6661 cacctggatg cagcgcacca ccatgggctg gtttgtctac cggctcacca attcagcctt
     6721 cctgctggga gtgatcagct tcctcagcat gatcccggcc ctctttatca gtccctttgc
     6781 cggggcctgg ggagaccggt ggaaccgtca tcacactctg atcatcaccc agagcctgtt
     6841 tatgctgcag gcagcgctgc tggcattagc agccatcagc ggatttatca atgccgacag
     6901 gatctggccg ctgatggccc tcagcttgat gcagggagtg atcgaagcgg tggattcacc
     6961 catccgccag agctttgtga tcgatctggt agccaaacgc agcctgctgc ccaatgctat
     7021 cgccaccaat tccgccatgt tcaacagcgc cagactggtg gggcccagtt ttgggggagc
     7081 gatgatcctg ctgtttggag aggggatgtg ctttgccatc aatgcgatat cctatatccc
     7141 cgtgatcgtg atgctgttta tgatccatct cagttacccg gcccgaaaac cccaatccga
     7201 gagcaccttc accaagatca aagagggctg gaactacgct ttcggtcatc tgcccatccg
     7261 tttcctgatc tccaatctaa tggtgtttac cctctttggg atgtcctatt ccactttgat
     7321 tcctgtcttc gcgcgggata ttctcaaagg cactgccggc acgcaaggtt tattgctatc
     7381 cacggctggg atcggagctt tgtgcacctc cttttatctg gccagccgca agactatcaa
     7441 ggggttgccc actgtgatga tcagtctggg agtggccgtg agcctggtgc tgatcatctt
     7501 ttcacgcagt tcgtctttgc cgctcagcct gttcctgatg ctctttatcg gcctgggcat
     7561 gaccatgcag atggcatcca ccaacaccat tatccagtct gtggtggcag accatatgcg
     7621 gggccgggtc ctgagcgttt acacgatgtc ttttatggcc gcgtctccct ttggaggctt
     7681 gctggcagga acgctgaccc ggcagtttgg tccccgcaac gcgctcagca tctgcgcggg
     7741 agtttgtctg atctgggcct tgtttggtct cagattgatt ccaaacatcg tgcgcgacat
     7801 cttcaggatg ctgatcatta gcggcaacaa agaaatctac aggccagtgg cggtaaagct
     7861 aaacccgggg gaagtgtgaa gacctggctg catttgggca acgggactcc agattaccgc
     7921 agcattatgg ccgcccacca tgacttctac atcggggata agctgattcc cgcttccaga
     7981 gtggtgatct gccagcagac ccattccagg gaagtgcata tctgtagcga agcggacagc
     8041 ggggccggtt ttggagctca tcctcagatc gcggtcgcgg atggttttgt gaccaatgtg
     8101 ccccatcagt atctcctgat caggacggcg gactgcactc cggtgctgtt tacagacaag
     8161 cttcacagag ccgtgggagc agttcacagc ggcagggaag gcacccggaa aaacatcgtg
     8221 agccgggctg tggaactcct gaaaagcgaa tatggcattg agccgcgtga cctctgtgcc
     8281 tggattgggg cgggaatctg tgtggaccac tatcaggtga accgggccgt ctatgacgcg
     8341 tttcacacca gcttgataga gacaggcata agtccagggc cgcgggatga tctcaaactg
     8401 gatatccgcc actgtatttt tcagcagctc atcgcagccg ggatcgcgtt tcgcgatatc
     8461 gagcaggacc acagctgcac ctttgagacc cagggttacc actccttccg cagagatggc
     8521 acccacaacc gtcagatcaa cctcgtcggg atcgaatatg actgatacct ataccaatcc
     8581 atccggcaat ctgagcctta gaatcagcga agacaaactc tccgcctggc tgaccgttca
     8641 cacgagcggc aagctgatca acgaacgtga catcctggac ctgatagacc gggcaggcat
     8701 caaatatggc tttgaggacg cgctccgata catccgggcc cacaatctcg aaaaggattt
     8761 tgatcagccc tttccggtgg ccatgtgcaa aatcggtggc agcggtaata ccatcaaacg
     8821 ctttttcgac gtggagcagg ccagagcttt caatcccaga gtgacacccg agcagattcc
     8881 aagcctgacc tgcgtggaag cggggacggt gctggccgat ctgagtgaca acatcttcga
     8941 tcgggagggt agcatctaca acgtgctggg cgagatgatc gctcccactc agacagatct
     9001 ggatgccgcc acggccatgg cgggagagaa cgtgagctat ctgccagaaa aggggcagtt
     9061 tgtgagcgtc aaatccggtt acatagcttt ggacgatgaa gaccgcgtga gcgtgcactc
     9121 caccctgatc atccagggcg acctggaagg ggttcaggac ctccgggccc ccgtcgattt
     9181 gatcgtggaa ggctccatcc ggggttctgg catccatctt caaggcggac tcagcgtttc
     9241 cggtgatatt tatgaaagcg gcatcagctg cggaggcgat ctcctggtag gaggtgaaat
     9301 ccgttcctgt cgtcaagggg gcctggaggt gatcggagat ctgcgctgcg gcaggatcat
     9361 cagctccagg gtgctctgca gggggctgct aagctgcagc gagatcaggt cttccgaagt
     9421 ggtgggggag aagggcatca gcactcaggc cgatggtccc atcattgcca gcaacgcgca
     9481 aagcgctgtc cgcattgaag ctggtcatgc cggggaagca gacgacgaga gcgaaacggt
     9541 gttggagatc acgatcagtc cttttctcaa agccctgatg atgcatttga cccgtgaact
     9601 ggtgcacgcc aagcaaaacg gccggcagga agaagtgaag gaaattgccg cccggatctc
     9661 cacctgcgaa catgaactgg acagagaact gaacgatttt ttggtttgcg agagcgacca
     9721 gctccgtggg gtcaaaatca tcggcaatgt ctatccggtc ctgaaagtcc gggtcttgaa
     9781 acatgagtat aaaatcagca aataccagag tgggctcagc atcgatgaga agcagtgaga
     9841 gctgcgctgt gagagctgcg ctggttagag cttcgctggt tagagctttg ctggttagag
     9901 cttcgctggt tagagctttg ctggttagag ctgcgctggt tagagctt
//
LOCUS       DAOSFN010000091         9896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5828_length_9896_cov_10.101311, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000091 DAOSFN010000000
VERSION     DAOSFN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5828_length_9896_cov_10.101311"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..2524)
                     /locus_tag="PLO35_06735"
     CDS             complement(182..2524)
                     /locus_tag="PLO35_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH79399.1"
                     /translation="MAKLLLTGASGLVGSHIAELFAHEGIPFKCLVRPQSDVSFLKQL
                     QAETVEGDITSISSVQNALDGVDCVIHAAGKASDWGRYRDFYTANVVGTMNVLRACKE
                     AGIANIIITGSISSYGEEDSQVIKNEDSPHKSHYPYFLDGLFPSAMNYYRDSKALLSQ
                     RAAHYAAEHKLNLSILEPAWVYGEREFNSGFFEYVKAVESGLRYAPGSKRNLFHVIYA
                     PDLAQAYLRAYRKQLPGVHRIIIASPKAHRLNDIHSLFCRAAGLPPPKLLPKFLVYPI
                     GLGMELLATLLSRKEPPLLTRSRVNMMYDNIAFSGERARQLLGWEATTALEDGIRQSV
                     DWYTQNGLLKKRQRRHQMSRHKLHGVHKELFILKVKLQVFYHFLRVYLQDHRLRTFIF
                     TLRRLNYFIGKISHNKFAQIGPYIRLDMYVPGFPSKAFYTACHKFTEFKGKLPCTVAL
                     LSVTSACTYHCEHCYQRLDHGKDVELDKLISAAKYLQNSGVAFFNIEGGEPFLTFDRL
                     LALCRAIDDRSEIWINSTGYGITKEKLLQLKSTSLSVIMFSLHTHDEGTLNAFMGDAH
                     AWQNMVNAIELCHQLDIAVAFNACLPLADFRNGNFEKLMQKAHDLGGILVQIIKPKPS
                     GAWLGKGVEEYSARDFELIKAKVNRYNLRPEFAHFPAISAQIMEEDPNLFGCTAGGTD
                     RLYINAKGDVQPCEFLNVSFGNIASEDFAIIYQRMRAVFEIPQTCICCERYAQDIYQL
                     YHDNHLQKLPLPPELSSQIFAKMRRDCPTPLYQKIERLLK"
     gene            complement(2517..3776)
                     /locus_tag="PLO35_06740"
     CDS             complement(2517..3776)
                     /locus_tag="PLO35_06740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOH79400.1"
                     /translation="MKRRVVITGIGPVTSLGIGKAVFWENLLAGAKPEIGQVPACKAN
                     TKSRVYVPFPQFSITDYGIPAYYNFLQPEDKLAILGTKLALEDAGFVITADNKTFRVA
                     ETSDIATIIGTGFTGLETAFFSYLAHLGLDYYCDRQHKKVSYNRMVIPLLMNNSPAAW
                     TSILFGLKGEGYTASASCASGTCALGQAYRHIADGYADLAISGGVENLQDENFAIFRG
                     FDVLGALTRSESGDPQPFSQSRSGFLFAEGGGCMLVLEELDHARRRNANIYAEILAYH
                     ANCDAHNIVNIDPEGRQILALLKHLSSGRKIDYLNAHGTATLISDAIEAQVIIDCFGD
                     NATQPLINSTKGILGHTIGGSGAIEAAVTALSVFNGKVHANQVDAPIPNLNLAMETHE
                     MPIQTALSVSYGFGGHNAGLLLGKYNG"
     gene            complement(3773..4789)
                     /locus_tag="PLO35_06745"
     CDS             complement(3773..4789)
                     /locus_tag="PLO35_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOH79401.1"
                     /translation="MSYESSLIALINGVMPPSPLRKSSPGEVDAELIRLGDKDFLFTT
                     DDFSAEDLLREDQPFELGWNLACGAISDIIAAGGKALVYAHAMVIPATWDEPYLREFS
                     RGISAVLGQYSVSFIGGDLGRGQTWRYTASVIGVPEAKTLTRRGCRAGDAIMITGRIG
                     AGNLMAAMGLFPDIQAASLKDATLRFSTHPKLAEIISPCASSAIDTSDGVFAALQTLC
                     DLNGTGFSLGKLPLHDQGLLASRLLGLPDLLLFLGECGEYEILFTVSQPHKKQLMADL
                     CKQEIEIHALGQITANPGQKTVLHEDRHYDLDDYRIKARDFESVSAYLKAMTVWLEKR
                     GARA"
     gene            complement(4779..5714)
                     /locus_tag="PLO35_06750"
     CDS             complement(4779..5714)
                     /locus_tag="PLO35_06750"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOH79402.1"
                     /translation="MDYFFIINPGSRHRGGARFIPALLKTLQIRRIDFDFRITETMQD
                     ACRFSREANLKGAAVVVAVGGDGTINQVINGFYDDDGKRISSSKLGVIHTGTSPDFCR
                     SYGIPTQPELALEALLRGFTQEVSVARIEYHDQSGSSRRGYFACCASFGLGAQVARAA
                     NSGLRNILGDTLGTFASILLALGRYKASDLVLHCDGNPVVIERNFNTFIGKTAWIASG
                     MKVAHELACDDKRLYILAVKKINIHNLVPALRAIYSGKAIRKQDYITFCYAQTIEIQQ
                     GSINNEIEFDGDPQGYLPCRISVAADPLEVITHEL"
     gene            complement(5719..6912)
                     /locus_tag="PLO35_06755"
     CDS             complement(5719..6912)
                     /locus_tag="PLO35_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOH79403.1"
                     /translation="MSFRDKFFHDFAEEDRLVHSLGFNPYYQTIDSGADLYVRINGEP
                     YLNLASNNYLALASHPELITAMKLALDIYGASMCGTPVACGKVDIYQSATHAIAAFLG
                     LEDAILYPSCYQANVAVIRALVKPPDLVFVDRNAHSSLVEGVRASGCKIKPFKHNDVD
                     YLAALIARSQGFEKRFVITESVFSTEGSIAPFDEIYRLALEHDVIPVIDDSHGIGVIG
                     ASGKGILEEKHIASYEGVYTASTGKALGVAGGIVSGNAQIIEYLRYSSPGLLYSTALA
                     PTLIAGTLKALEIVARDGIKLVRALADNQRYLHENLQRQGFAMLPSEAFICSLSAGSN
                     ETSFRICKALFDAKILTTPFIYPSVAKNRGVIRMIPRVDLSREQMDYVLRTFARIRTE
                     HPELF"
     gene            complement(6896..7144)
                     /locus_tag="PLO35_06760"
     CDS             complement(6896..7144)
                     /locus_tag="PLO35_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH79404.1"
                     /translation="MDTLDAVITIIRNNISSDADISADSDMRKDLGVDSFDGLMIMNE
                     IDDAFGISVDEDDFKKVNSPQEIVDLLKDKYGIHELQR"
     gene            complement(7162..8340)
                     /locus_tag="PLO35_06765"
     CDS             complement(7162..8340)
                     /locus_tag="PLO35_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOH79405.1"
                     /translation="MSSAEIFQDKLFHSATFEGMADANGAPGEEYLEFYRELAAQGVR
                     NIITGCTYVSREGKMVQPGQAGMDEDARIAPFEMVTAAVHRHNAKMYLQISHAGRQTS
                     SSVTGLQVVGASAQRSPYFLSRPKQLSIGEIGQVIEDYAAAALRARHAGFDGVQIHAA
                     HGYLLHQFLHPGINNRSDAYGINPMTGIGDLFLRKLIAAIRASCGSSYPILVKVSAAD
                     DLPKGFSLSNFISLIRLLHSERVHAIEISYGTMEDALNIFRGASIPIDVILRHNFRYK
                     QANPAIRRLWKLLVLPVLKHRLIPFRANYNLPFARMAKRITDIPVICVGGFRKGSEIL
                     KAIESGQTDYVSLCRPFIREPDFVAKLCRDSEYESRCVNCNLCAVMCDSGRPTRCYKR
                     "
     gene            complement(8340..9317)
                     /locus_tag="PLO35_06770"
     CDS             complement(8340..9317)
                     /locus_tag="PLO35_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HOH79406.1"
                     /translation="MLRFMRLYIRSMRLYYSFVTGIAGWLGFAFYEHIATDFRTVEIM
                     PSLPRKILIITMLFFSWGINQIVNDYLGLKEDRINAPDRPMVTGELNHVSALLLSASL
                     MLLVFLLTWLVLEKIAVIPLILGLILNVVYEYAKSEGIMGNIVFGLMITMCCAYGFLA
                     AGPTQAPYFTPSRLSVIAMVWLMNGLMTFYTYFKDYKGDRRAGKNTIIVKYGLRTSRY
                     ISIFSAFLPALLFVGLYASGLIDAALNPTFVALAILTFALEIQTGWLYFKNPVGELTY
                     YSLKTNFRACTCGQATFVALFNPPLGLLLFVLSYLMVGFLFGMHHNRKA"
BASE COUNT         2223 a         2644 c         2782 g         2247 t
ORIGIN      
        1 aggctggttg gcatatccta cccccccccg tttcgcgatg cttcacgggg ggctattaca
       61 tttggacgcc ctccagggca tcaaagtggc tatttccgca caagctcttc agccggtcgc
      121 agctctgact acagagcttg cctgtgtaca agtgacgcgg ctcgctgccc ctcaagcagc
      181 gtcacttcaa tagccgctcg attttttgat acaggggagt gggacaatcg cggcgcattt
      241 tggcaaagat ctggctggac agctccggag gaaggggcaa tttctgcaga tgattatcat
      301 gatagagctg gtagatgtcc tgggcatagc gttcgcagca gatgcaggtt tgcgggatct
      361 caaacacggc tcgcatgcgc tggtagatga tggcaaaatc ctcactcgcg atgtttccga
      421 aggagacatt gagaaactca cagggctgca catccccttt ggcattgata tagagccggt
      481 ccgtgccccc cgcggtgcag ccaaagagat tgggatcttc ttccatgatc tgggctgaga
      541 tggccgggaa gtgggcaaac tccggcctta ggttatagcg gttcaccttg gctttgatga
      601 gctcaaaatc ccgcgcggaa tactcttcca ccccttttcc aagccaagcc ccggagggtt
      661 tgggtttgat gatctgcacc aggattcctc ccaggtcatg ggctttctgc atcaactttt
      721 caaaattgcc gttgcggaaa tcggccaaag gcagacaggc attgaaggcc acggcgatgt
      781 caagctggtg acacagttca atggcgttca ccatattttg ccaggcgtgg gcatcgccca
      841 taaaggcgtt gagggtgccc tcgtcatgtg tatgcagcga aaacatgatc acgctgaggc
      901 tggtgctttt aagttgcagc agcttttctt tggtgatgcc gtaaccggtg gaattgatcc
      961 agatctcaga gcggtcatcg atggcccggc acaaagccag cagcctgtcg aaggtcaaaa
     1021 acggctcgcc cccctcgatg ttaaaaaagg ccactccgct gttttggagg tatttagccg
     1081 cggagatcag tttatcgagc tccacgtctt tgccgtggtc caagcgctga tagcagtgct
     1141 cgcagtgata ggtgcaggca gaggtgacag acagcaaggc caccgtgcag ggcagtttcc
     1201 ccttgaattc ggtgaatttg tggcaggcag tataaaaagc tttggaagga aagcccggca
     1261 catacatatc cagacggata taggggccga tttgggcgaa cttgttgtgg gaaattttgc
     1321 cgataaagta gttcaagcgc ctcagcgtga agatgaaggt cctgaggcgg tgatcttgca
     1381 gatacaccct caggaagtgg tagaaaacct ggagcttgac tttgagaata aagagctcct
     1441 tatgaactcc gtgcaacttg tgccggctca tctgatgcct cctttgccgc tttttgagca
     1501 gtccattttg cgtgtaccag tccacgcttt gccggatgcc gtcttccaga gcggtggtag
     1561 cttcccaacc cagcagctgc ctggcccgct ccccagagaa cgcgatattg tcatacatca
     1621 tattcacccg cgatctggtg agcagtggtg gctccttgcg ggataaaagc gtggcaagca
     1681 gctccatccc caggccgatg ggatagacca gaaacttagg cagcagcttg ggaggaggaa
     1741 gccctgccgc cctgcagaac agggagtgga tatcattgag tctgtgagct ttgggacttg
     1801 cgatgattat ccggtggacg ccaggcagtt gcttccggta ggcccgcaga taggcctggg
     1861 ccaggtctgg ggcataaatc acgtgaaaga ggttgcgttt gctgcccgga gcatagcgca
     1921 gaccggattc gacggctttg acatactcaa agaatccgct gttgaattcg cgttcgccat
     1981 acacccaggc cggctcgagg atgctgagat tcagcttgtg ttccgcggcg tagtgagcag
     2041 ccctctggct cagcagcgct ttgctatcgc gataatagtt catggcggaa ggaaacagcc
     2101 cgtccaggaa gtagggataa tgggacttat gtggagaatc ttcatttttg atgacctggc
     2161 tatcctcctc cccgtaagat gagatggaac cggtgatgat gatattggct atgccggctt
     2221 ccttacaggc cctcagcaca ttcatggtac ccaccacatt cgccgtatag aaatcccgat
     2281 agcgtcccca atcagaggct ttgccggcgg cgtggatcac gcagtccact ccatccagag
     2341 cgttttggac agaacttatg ctggtgatat ccccctcgac ggtctcagcc tgcaactgct
     2401 tcagaaagga gacatcgctt tgaggtctga ccagacattt gaagggaatg ccttcgtggg
     2461 caaacaattc agctatgtgg ctgcccacca agccactcgc cccggtcagc agcagcttag
     2521 ccattgtatt tccccaagag cagacccgcg ttgtgtccac caaaaccgta ggaaacgctc
     2581 aaagcagtct ggatgggcat ctcatgagtt tccattgcca ggttcagatt gggaatggga
     2641 gcgtccacct ggttggcatg gaccttgccg ttgaaaaccg acagggccgt gacggctgct
     2701 tcgatagctc cgctgccgcc gatggtgtgg cccaaaatgc ctttggtgga attgatcagc
     2761 ggctgggtgg cattgtctcc gaagcagtca atgatgacct gggcttcgat ggcgtcgctg
     2821 atcagagtgg cggtgccgtg agcgttcaga tagtcgatct ttctgccaga gcttaagtgc
     2881 ttgaggagag ccaggatttg ccgcccttcc ggatcgatgt tgacgatgtt gtgggcatcg
     2941 cagttggcgt ggtaagcaag gatttccgca tagatattgg cattgcgccg cctcgcgtgg
     3001 tccagctctt ccagcaccag catgcagcca cctccttcgg caaagagaaa gccgctgcgg
     3061 ctctgtgaaa agggttgggg atcaccgctt tcagacctcg tcaaagctcc cagcacatca
     3121 aagccccgga agatggcaaa gttctcatcc tgaaggtttt ccaccccgcc gctgatggca
     3181 aggtccgcgt agccatcggc aatatgacgg taagcctgcc ccagcgcgca ggttccggaa
     3241 gcgcaggagg cgctggcggt gtagccttct cccttcagac cgaagaggat ggaagtccag
     3301 gcggcgggtg agttgttcat caggaggggg atcaccatgc ggttgtagga gacttttttg
     3361 tgttggcgat cgcagtaata atccaggccc agatgcgcca gataagagaa aaaggctgtt
     3421 tccagcccgg taaatcccgt gccgatgatg gtggcaatat cggatgtctc cgctactctg
     3481 aaggtcttat tatcggcggt gatgacaaat cccgcgtctt ccagggccag tttggtgccc
     3541 aggatggcga gcttgtcttc cggttgcaga aaattgtagt aggcgggaat gccgtaatcc
     3601 gtgatggaga actggggaaa ggggacatag acccgcgatt tggtgttggc tttgcaggcc
     3661 gggacttgcc ctatctccgg tttggctccc gccagcaagt tttcccagaa cacagctttg
     3721 ccgatcccca aagaagtcac aggtccgatc ccggtgatca ctactcttct tttcaagcgc
     3781 gcgctcccct cttttcaagc cagacggtca tcgccttcag gtatgctgac acgctttcaa
     3841 aatctctggc tttgatgcga tagtcatcca gatcatagtg gcgatcttca tgcaggaccg
     3901 tcttttggcc aggattggcg gtgatctggc ccagcgcgtg gatttcaatc tcctgcttac
     3961 atagatcggc catcaattgc tttttgtgtg gctggctgac ggtgaagagg atctcatatt
     4021 ctccacattc tcccaaaaac agcagcaagt ccggcaagcc cagcagccgg gaggcaagca
     4081 agccctgatc atgcaaaggg agcttgccga ggctgaagcc agtcccgttg aggtcgcaga
     4141 gcgtttgaag ggccgcgaag acgccgtcgc tggtgtcgat cgcgctggag gcgcaagggc
     4201 tgataatctc cgccagtttg gggtgggtgc tgaatctgag ggtggcatct ttgagggatg
     4261 ctgcctggat atctggaaac aggcccatgg ccgccattag attgccggcc ccgatcctgc
     4321 cggtgatcat gatcgcgtct ccggcccggc agcctctgcg ggtcagcgtt ttagcctctg
     4381 gaacgccgat cacggaagcg gtgtagcgcc aggtttggcc tctacccaaa tcacctccga
     4441 taaaggaaac ggaatattgg cccagcaccg cggaaatgcc tctggaaaac tcccggaggt
     4501 agggctcgtc ccaggtggct gggatcacca tggcatgggc atagaccaga gctttgcctc
     4561 ccgcggcgat gatatcgctg atcgctccgc aggcaagatt ccagcccagt tcaaagggtt
     4621 gatcttcccg cagcaagtcc tcagcggaaa agtcgtccgt cgtaaagaga aagtctttgt
     4681 ctcccaagcg gatgagctcg gcatccacct ctccggggct gctcttgcga aggggagagg
     4741 gaggcatcac gccgttgatc agtgctatca ggctgctttc ataactcatg ggtgatcact
     4801 tccagggggt cggctgccac agatatcctg cagggcagat agccctgggg atcgccgtca
     4861 aattctatct cgttattgat gctaccctgc tgaatctcaa tcgtttgggc ataacaaaag
     4921 gtgatgtagt cctgcttgcg gatggccttt ccggagtaga tggccctcag ggcagggacc
     4981 agattatgga tgtttatctt ctttacagca agaatataca aacgcttgtc gtcacaggcc
     5041 agttcatgcg ccaccttcat gccggaggca atccaggcgg tcttgccgat gaaggtgttg
     5101 aaattgcgct ctatcaccac tgggtttccg tcacagtgca ggaccagatc agaggctttg
     5161 tatctgccca gggccagcag gatggaagca aaggtcccaa gcgtgtcacc caggatgttc
     5221 ctgagcccgg aattggccgc ccgggccacc tgagccccca ggccaaagct ggcacagcag
     5281 gcaaagtatc cgcggcggct tgatccgctt tggtcatgat actcgattct ggccacggag
     5341 acctcttggg tgaagcccct cagcagggct tccagggcca actccggttg ggtggggatc
     5401 ccatagcttc tgcagaaatc cgggctggtc ccagtgtgga taacccccag ttttgagctc
     5461 gatatccgct tgccgtcatc gtcatagaag ccgtttatca cctggttgat ggtcccgtct
     5521 ccacccacgg ccactactac cgcggcacct ttcagattgg cttcccggga aaagcggcag
     5581 gcatcctgca tagtttccgt gattctgaaa tcaaaatcta tgcggcgtat ttgcagggtt
     5641 tttagcaatg ccgggatgaa tctggccccg ccgcggtgac gggagccggg attgatgatg
     5701 aagaagtagt ccatgatctt agaacagctc cggatgctct gttctgatcc gcgcaaaggt
     5761 ccgcagcaca tagtccatct gctcccggct caaatccacc ctggggatca ttctgatcac
     5821 tccccggttt ttagccaccg agggatagat gaaaggggtg gtcaggatct ttgcgtcaaa
     5881 cagagcctta caaatcctga agctagtctc gttggatcca gcgctcagcg aacagataaa
     5941 agcctcactg gggagcatcg cgaagccttg ccgttgcagg ttttcatgga ggtaacgctg
     6001 gttgtccgcc agagcgcgca ccagcttaat gccgtctctg gccacaatct ccagagcttt
     6061 gagggttccg gcgatcagag tgggggccag ggcggtggaa tagagcagtc ccggcgaaga
     6121 atatctcaag tattcgatga tctgagcgtt gccgctcact attccacccg ccactcccag
     6181 agctttgccg gtggaagctg tataaactcc ctcatagctg gcgatgtgtt tttcctccag
     6241 aatgcctttg ccgctggcac cgatcactcc aatcccgtgg gaatcgtcga tgacgggaat
     6301 cacatcatgc tccagggcaa gccgataaat ctcatcgaag ggcgcgatgc tgccctcggt
     6361 ggaaaacacc gattccgtga tcacaaagcg cttttcaaac ccttgcgacc tggcgatgag
     6421 agcggccaga tagtccacat cgttgtgttt gaaaggcttg atcttgcatc cgctggcccg
     6481 gacgccttcg acgagggagg aatgggcatt gcggtccaca aacaccaggt ccgggggctt
     6541 taccagcgcg cggatcacag ctacattggc ttgatagcag gagggataga ggatcgcgtc
     6601 ttccaggccc aggaaagcgg caatggcgtg ggtggcggat tgatagatat ccaccttgcc
     6661 acaggccacc ggtgttccac acatggaagc gccatatata tccagggcca gcttcatggc
     6721 ggtgatcagt tccgggtgag aggccagggc cagatagttg tttgaggcca gattcagata
     6781 gggctcaccg tttatcctca catagaggtc ggcgccggaa tcgatagtct ggtaataggg
     6841 gttgaatccg aggctgtgca ccagcctgtc ttcctcggca aagtcgtgga aaaacttatc
     6901 tctgaagctc atggatgcca tacttgtctt tgagcagatc tactatttcc tgaggagaat
     6961 tgactttttt aaagtcatct tcatccaccg aaatgccgaa ggcatcgtcg atctcgttca
     7021 tgatcatcaa gccatcaaag gaatccacac ccaggtcttt gcgcatatcg ctgtccgcgc
     7081 ttatatcggc atcgctgctg atgttgttgc ggatgatggt tattactgca tcgagcgtat
     7141 ccatatcggt ctcctgtgat ctcatctttt atagcacctg gtgggcctgc cggaatcgca
     7201 catgacggcg cagaggttgc aattcacgca cctggattcg tattcgctgt cccggcagag
     7261 tttagccaca aaatccggct cgcggataaa aggccggcac aggctcacgt agtcggtttg
     7321 gccgctttcg atggccttaa gaatctcgct gcccttgcga aagccgccca cacagatgac
     7381 cgggatgtcc gtgatccgtt tcgccatgcg cgcgaagggc aggttatagt ttgctctgaa
     7441 gggaatcaat ctgtgtttca aaaccggcag gaccagcagc ttccatagcc gcctgatagc
     7501 tggattggcc tgtttgtagc ggaagttatg cctgagaatc acatcgatcg ggatggacgc
     7561 tccccggaag atattgagcg cgtcctccat ggtgccatag ctgatctcta tggcatgcac
     7621 cctttcagag tgcagcagcc tgatcagaga gataaaattg ctgaggctaa agcctttcgg
     7681 caaatcgtcc gccgcgctca ccttgaccag gatgggatag cttgatccgc agcttgctct
     7741 gatggccgcg atgagcttgc gcaaaaacaa gtccccgatt ccggtcatgg ggttaatgcc
     7801 atacgcgtcg cttctgttat tgatgccggg atgcagaaac tgatgcaaca gataaccatg
     7861 cgcggcatgg atttgaactc cgtcgaagcc cgcatgtctg gcacgtaaag ccgccgcggc
     7921 ataatcttct atgacctgcc cgatctcccc gatggaaagc tgtttgggcc tgctcaggaa
     7981 atacggagac cgctgagccg acgcgcccac cacctggagt cccgtcacag agctggaagt
     8041 ctggcgcccg gcgtgagata tctgcagata cattttggca ttatgccgat ggaccgcggc
     8101 cgttaccatc tcaaacggcg ctatcctggc atcctcatcc atgccggcct gaccgggctg
     8161 gaccatcttg ccttcccttg agacgtaagt gcagccggtg atgatgtttc gcactccctg
     8221 cgcggccagt tcccggtaaa actccagata ctcctcaccg ggagcgccat tcgcgtccgc
     8281 catcccctca aaggtggcag agtggaagag cttgtcctgg aaaatctcag cggagctcat
     8341 caggctttcc ggttgtgatg catgccaaag aggaatccca ccatcaggta ggagaggacg
     8401 aagagcagca gccccagcgg ggggttgaac agcgctacaa aggtagcctg tccgcaagtg
     8461 caggctctga aattggtctt gagagagtaa taagtcagct ctcccaccgg gtttttaaaa
     8521 tacaaccagc cggtctgaat ctccagagca aatgtcagga tggccagagc tacgaaggtg
     8581 gggtttaagg ccgcgtcgat cagccccgag gcatagaggc ccacgaacag cagcgccggc
     8641 aggaaagcgg aaaatatcga gatatagcgg gaggtcctga ggccgtattt cacgattatg
     8701 gtgtttttcc cggcccttct atcgcccttg tagtctttga aataggtgta gaaggtcatc
     8761 aatccgttca tcagccacac catggcaatc acagacaggc ggctgggagt gaaatagggt
     8821 gcctgagtag gtcccgccgc cagaaaacca taggcgcagc acatcgtgat catcaggcca
     8881 aacacgatgt tgcccataat cccctcagac ttggcgtatt catagactac attcaggatc
     8941 aggcccagga tcagagggat gacggctatc ttttccagaa ccagccaggt gagcaaaaac
     9001 accagcagca tcagggaagc ggaaagcagc agggcggaca cgtggttcag ctcacccgtc
     9061 accataggcc tgtctggagc gttgatccgg tcttctttga ggcccagata gtcgttcacg
     9121 atctgattga tgccccagct aaaaaagagc atggtgatga tgaggatttt tctgggcagc
     9181 gaaggcatga tctccacggt cctgaagtct gtggcgatgt gctcataaaa cgcgaatccc
     9241 agccagcccg cgatccccgt cacaaaggaa taatagaggc gcatggagcg gatgtagagc
     9301 cgcataaatc tcagcacggt atcttccctg ggctcatgcg cgtcgccccg cgctccggat
     9361 ctttcctttt atcccatctg gatccaggaa acatcgctgc atcgcgctta cgagcgtcat
     9421 cattagcggg cctcagaatc atattccatc ccatgctgtt cagtttgctt gagccggctc
     9481 ttgagctcgt ggtggcatga atttgccagg cactgagttc agttaaagat gggcttcatg
     9541 ttactttcgt tccaaatcgc ctgaattcat cccgttggag tggaaactgg cagacacccc
     9601 ggggccacaa agccggacag gcaactacac atataataat caatttcaca tcgccgtaac
     9661 actggcttca gccggtcgtt gcaatgactt tagtcggtgc attatacgcg taaccacaaa
     9721 caaactcaga gaaagcggag aaaacagagg gtttgtctta caatgagaca tgagaatatg
     9781 agagtgtatc caactatgag accatccgta acgctggctt tagaacttgt gcgaaaatgg
     9841 tgcattgagt cccatgggga cgcaggctgt tgacaaagta tcaaatcaga tagaac
//
LOCUS       DAOSFN010000092         9892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5831_length_9892_cov_8.515503, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000092 DAOSFN010000000
VERSION     DAOSFN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5831_length_9892_cov_8.515503"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /gene="tgt"
                     /locus_tag="PLO35_06775"
     CDS             <1..691
                     /gene="tgt"
                     /locus_tag="PLO35_06775"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOH79407.1"
                     /translation="MSFDECPPYPATREYVEKSLKTTLRWAEQGKRAFTNDQHQALFG
                     IVQGGVYEDLREKSARALMDIDFPGYSIGGLAVGESKPDMLRVTSFLNDILPMDKPRY
                     LMGVGTPADLLNNIANGVDMFDCVMPTRNARKGSIFTRYGKMIIKAARYKDDFNPIDP
                     LCNCYTCRHFSRAYIRHLITMNEILGMRLTTIHSLYFYQELMQMARQAILENRYEDFL
                     AEMLPVLDLVI"
     gene            785..2149
                     /locus_tag="PLO35_06780"
     CDS             785..2149
                     /locus_tag="PLO35_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOH79408.1"
                     /translation="MNRIRLLLPLLMLAAILVLSAETKPPKLFTAPLLQDNVTPDMLN
                     PGFWISRHPSPDAVIMNPDQIVKFNTSVRNRRGSVANLPNLTGSIAGRTIRNGIENGL
                     KVLRNMRLYDANGARVPDSYWGPIKANCNLGSIPGSIKIRTALAAVFADQRIVPSDVN
                     FNKKAFDYEFDELQNSGYDIGTPLVIYHTSADGLWAYGSSAVTSGWFRVSELCFVDQA
                     VWKDYQNALDFAVVISDRAEVWQDSLSTRWSGFARMGSRFPLLGEKGDNYIINLPVRD
                     SLGSRLISGYISRRDCQRGYLTYTARNVYLLAFRMLDRPYGWADMHGNTDCSSFIRQL
                     FACFGIVLPRNSAEQEKSGKIAYSFKSGETALKRNDLLIKHGKPAITVLRRPGHVTLY
                     LGSVDGRGYIIHNTWAERMKNDKKSNDIHVIDRVVVSDVFIGDKSTLGPMIKHFSAVS
                     VLKN"
     gene            complement(2363..2569)
                     /locus_tag="PLO35_06785"
     CDS             complement(2363..2569)
                     /locus_tag="PLO35_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79409.1"
                     /translation="MKKVLLLTLVAMMLLSMLVMSACKPKAEEAPVDEAAPVEEVTPA
                     PVDTTQVPADAAAPAATTPAPAGK"
     gene            complement(2782..4452)
                     /locus_tag="PLO35_06790"
     CDS             complement(2782..4452)
                     /locus_tag="PLO35_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOH79410.1"
                     /translation="MKRTLPLFLLLWLSVLSGLSVQKLSWDADFPVDAAAWEQASGII
                     GGAVYDPATIREAITRLEQYLSSSGRYFVKIPFPELIPVADTLIELRFSLREVLPSDQ
                     MKLRLAGMRYFSEDRLKEMLLISEDKRFAIKELDRVIGQILDICHSRGFLFARVELDS
                     LVLEQGLTAWLKVSEGKVFKPEKIFFQGNKYTRDRSLLKLSGIMSVRVITPQALEEAA
                     QNILRKPYIKDALVEPITDNSLLIRVEEGRMTYLEGVVGYNGKQKELSGLLKLKFLNL
                     WGSDRSLNLNWRQDSSRSELELGYHESGPGSIPLAGDLLLQRTSQDTLWIKSNISAKI
                     YGYYGYHKLGLELGSSDNLIDPSLARGPSQRIRAASLGAFWELDSAYPVGNPAKGYQG
                     RLAYRFLNSKDRGWNGAFELDWANFIPLGKRLVGAISLHFRDLDDVEARDYDLYQMGG
                     YEKLRGYNDGEFQSWRLGWVAWELRWRLDPASRLHVFFDHGLLARRLDGPLGPRTDYR
                     TDLFGFGLGIRIGTRIGILGIDYALGNREDGLSGLGAGMVHAGLNASF"
     gene            complement(4462..6258)
                     /gene="lepA"
                     /locus_tag="PLO35_06795"
     CDS             complement(4462..6258)
                     /gene="lepA"
                     /locus_tag="PLO35_06795"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOH79411.1"
                     /translation="MKQEFIRNFCIVAHIDHGKSTLADRFLEATHVVGKVDMAQVLDS
                     MDLEREKGITIKSHAIRMIHNYKGQDYVLNLIDTPGHVDFSYEVSRALASCEGAILLV
                     DASQGIEAQTMSNLYLALENNLELLPAINKIDLPKADVEGTEHDLTDILGCLPEEIAK
                     VSAKAGIGIDELLDKVVEVLPPPVGKEDAPPKALIFDSYFDMYRGVVVLVRLVDGKLR
                     KGDRVRLFSTSREYEIEEIGHLGLKFAPMQELVTGEAGYIIANIKEVADARVGDTITL
                     AKGGCAESLPGFQEPKPMVFSGIFPINGEDYENLVDSIAKLKLNDASLVYEKENSAAL
                     GYGFRCGFLGMLHLEIVKERMLREYNIPIIATTPSVRFLINLKNGEQIVVNNPIDFPD
                     PNYIDSIQEPFMDTEIIVPTDYIGNIMKLAQERRGIQKDIQYIDEKRVALHYEMPLIE
                     IIFDFYDKLKTVSRGYASLDYHFKEYRSSNVVKVDILINGEKVDAMSFICHADKAHNW
                     GKSVTATLAEVIPRHLFKIALQASIGAKIIARSTINPMRKDVLAKCYGGDVSRKRKLL
                     DKQKEGKKKMKEIGSVTVPQEAFLAVLKADRE"
     gene            6914..7342
                     /locus_tag="PLO35_06800"
     CDS             6914..7342
                     /locus_tag="PLO35_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOH79412.1"
                     /translation="MHPYAPACGLYCGACSSMLLHEKESGLSHAAHYIHEYNEGPCPG
                     CVNGAQECEFIRCAREKGVSGCAACPDFPCVMILKFSKDEWPHHIEVIDNLMRIREIG
                     EAAWLSEQAERWSCPDCGGRTHWYQKACTICGAVITSAYS"
     gene            complement(7356..8435)
                     /locus_tag="PLO35_06805"
     CDS             complement(7356..8435)
                     /locus_tag="PLO35_06805"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOH79413.1"
                     /translation="MNTLFRKDLLDKEPSVVNVPVRQRMMCLNESCLDPYQAIKDEFS
                     KRMETVRLNRYMSPVTGELHQKLAEYVGCGIKPENLLWGNGADDMLYHIFLSVRDGER
                     SFAVSPAPSYFDYKTFCEAVGLGIKFVELNEDFSLDTEAYLKAASHPDCSLAILCNPN
                     NPSGNLFPKEQLRQIVDALPDKLVLIDETYYEFSDSSFVDELASHPNLILTRSFSKAF
                     SGAGLRFGYAISSPDNIYSLRKVFTTFHSSILSQAFALTILENVPLFCGQVLDVIKLR
                     DRVYEELKALPGLTVHPSHTNFLTFTVGERTPRLFEYLKDQDIALRDVGAHRRLKNCL
                     RATLSCVDDTEELVRQIKLWMRSQA"
     gene            8829..>9892
                     /locus_tag="PLO35_06810"
     CDS             8829..>9892
                     /locus_tag="PLO35_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOH79414.1"
                     /translation="MRIHEILDRIESFAPLALAMSWDNCGLLLGEPQAEIDKVFVALD
                     VTPRTVKLAIEAGAQLILSHHPIIFRPLTKITDPLLLRLIEHRIAVISLHTNLDVAPG
                     GVNHALADALGLKVLEHLSPETGDSWNHLSVSVPATHAARLKEAVFASGAGLIGLYDS
                     CSTLHPVQGSFRALPGSQPFIEGEGLQEVDELELEFRVDGSRLGAVLKAIETTHPYQT
                     PALYHFPVANRNPAYGLGLICAPAEPADLPTLQKLCTERLGCPHPRLWTAGKDISAPI
                     ERIAICGGSGGSLIASASSKAQVYISGDFSYHTMMESRIPLIDAGHFYTEYPVLAVLA
                     KKMEDLGLNSVVMPMEECEWV"
BASE COUNT         2238 a         2708 c         2659 g         2287 t
ORIGIN      
        1 catgagcttc gatgaatgcc caccctatcc agccacgcgc gagtatgtgg agaagtcact
       61 caagaccacc ctgcgctggg ccgagcaagg caaacgggct tttacaaacg accagcatca
      121 ggccctcttc ggcatcgtgc agggaggcgt gtatgaggac ctcagggaaa aatccgcccg
      181 ggccctgatg gatattgact ttcccggcta ttccatcggc ggactcgcag tgggtgaatc
      241 caagcccgat atgctgaggg tcacaagctt tctaaacgac atcctgccaa tggacaagcc
      301 acgctatctg atgggagtgg gcactccggc agaccttctg aacaacatcg ccaacggggt
      361 ggatatgttc gactgcgtca tgcccacccg caatgctcgc aagggttcca tcttcacccg
      421 ctacggcaag atgatcatca aggcggcccg ctacaaggat gacttcaacc ccatcgatcc
      481 cttgtgcaac tgctacacct gccgtcactt cagccgggcc tacatccgtc acctgatcac
      541 catgaacgag atcctgggga tgcggctcac caccatccac agcctctatt tttatcagga
      601 actgatgcag atggcccgcc aggccatcct ggaaaaccgt tatgaagatt ttctggcaga
      661 aatgctgccg gtgctggacc tggtgatttg aggtcgtgaa gcgttacgcg ttacgtgatc
      721 ctgagtgcgt aacgggatga cgcctccagc tttagaagta tttcgaaact tatataggta
      781 tgttatgaat agaattagat tattgctgcc gctcctgatg ctggcagcca tcctcgttct
      841 gagtgccgaa actaagcctc ccaagctgtt tactgccccg ctgctccagg acaacgtgac
      901 ccccgatatg ctcaatcccg gcttctggat cagccgtcac cccagtccgg acgctgtaat
      961 catgaatccc gatcagatcg tcaaattcaa cacctctgtt cgcaaccgtc ggggttccgt
     1021 ggccaacctg cccaatctga ccggcagcat cgctggccgc accatccgta acggcatcga
     1081 gaacggcctg aaagtcctga gaaacatgcg gctctacgac gccaatgggg ctcgggtccc
     1141 ggatagctat tggggcccca tcaaggccaa ttgcaatctt ggcagcatcc ctggcagcat
     1201 caagatccgc actgccttag cagccgtctt tgccgatcag aggatcgtgc ccagcgacgt
     1261 caacttcaac aagaaagcct tcgactacga gtttgacgag ctgcaaaaca gcggctacga
     1321 tatcggcacc cccctggtga tctatcatac ctctgcggat gggctctggg cctatggttc
     1381 cagcgctgtg acctctggat ggttcagggt gagcgagctg tgttttgtgg accaggccgt
     1441 ctggaaagat tatcaaaacg cgctggattt tgccgtggtg atctctgacc gggcagaagt
     1501 ctggcaggac agcctttcca cccgctggag tggatttgcc cgcatgggtt cccgctttcc
     1561 tctcctgggt gaaaagggag acaactacat catcaatctg ccggtgaggg acagtctggg
     1621 ctccagactg atcagcggtt atatttccag acgcgattgc cagcgcgggt acctgaccta
     1681 tacagccaga aacgtttacc tcctggcatt caggatgctg gaccgtcctt acggctgggc
     1741 cgacatgcat ggcaacacag actgctccag ctttatcagg cagctgtttg cctgttttgg
     1801 aatcgtgctg ccacgcaaca gcgcggagca ggaaaagagc ggcaagatag cctatagctt
     1861 caaatcaggc gagacagctt tgaagcggaa cgatctgctc atcaaacacg gcaagcccgc
     1921 catcacagtc ttgcgccggc ctggccacgt aaccctctat ctgggcagtg tggacggcag
     1981 aggctacatc atccacaaca cctgggccga aagaatgaag aacgacaaaa aaagcaacga
     2041 catccacgtc atcgaccggg tggtggtgtc ggacgtcttc attggagaca aatccactct
     2101 gggccccatg atcaaacact ttagcgccgt atcggtgttg aagaactgaa gccgggactg
     2161 catagagaat ccctaagacc caaacgcgct atatattgac cctgattacc cgacaagtca
     2221 cagcatagct ctcacagcat agctctcaca gcatagctct cacagcatag ctcttaccag
     2281 ccaagttttt tatccccaaa caaaaaacgg gcacccgaag gtacccgttt tttaaggact
     2341 gcgctatagt tgctttagct tattactttc cagcaggagc gggagtggtg gcagctgggg
     2401 cagcggcatc agccggaacc tgggtggtgt caactggagc tggggttact tcttcgaccg
     2461 gagcggcttc atcaacggga gcttcttcgg ctttgggttt gcaagcgctc atcacaagca
     2521 tgctgagcag catcatagcg actaaggtca gtaaaagaac ttttttcatt gttgattcct
     2581 ccatttagtg aaatccatgt ttttttttgt tttgattttg tctttgtttg gcaatttaag
     2641 gccttgccag ggccttttgc ttgaggactg cttgaagccc gaaagccttt agcagccact
     2701 cacacagtcc ttacgcacaa ctatgtactg ttatattaga gcccacatta tccacccttg
     2761 gatttttggc aagagttttt tttagaatga ggcatttaat ccggcatgga ccattccggc
     2821 tcccaggccg gaaagcccat cttccctgtt tccaagggca taatcgatgc ccagaattcc
     2881 gatccgggtg cctatcctga tgcccagtcc aaagccgaag agatcggtgc gatagtctgt
     2941 cctgggcccc agggggccgt ccagacgtct cgccaagagc ccgtgatcaa agaagacatg
     3001 cagcctggaa gccggatcga ggcgccaacg cagttcccag gccacccagc ccagacgcca
     3061 gctctgaaac tcgccgtcgt tatagcccct cagcttttca taaccgccca tctgatagag
     3121 gtcataatcc cgcgcctcaa catcatccag gtccctgaag tgcagcgaga tagcccccac
     3181 caggcgttta cccagcggga taaagtttgc ccagtccagc tcgaaagcgc cgttccagcc
     3241 acggtctttg gagttgagga aacggtaggc cagcctgcct tgatagcctt tggcaggatt
     3301 gccaacggga tacgccgagt ccagttccca aaaagccccc agagaagcag ccctgatccg
     3361 ctggctggga cccctggcca gggagggatc gatgaggttg tcggagctgc caagctccag
     3421 tcccagttta tgatagccat agtagccata gattttggca ctgatgttgc tttttatcca
     3481 caatgtgtcc tgagaagttc tctgcagcaa gagatctccg gcaagcggaa tggaacccgg
     3541 accggattcg tgatagccca gctccagttc gcttcgggag ctgtcctgcc gccagttcag
     3601 gttcagggaa cgatcactgc cccagaggtt tagaaacttc agtttcaaga gaccgctgag
     3661 ctctttttgt ttgccgttat agcccacgac gccttccaga taggtcatgc ggccttcctc
     3721 gacgcggatc agcaggctgt tgtcggtgat cggttccacc agcgcgtcct tgatataggg
     3781 cttgcggagg atgttttggg ccgcttcttc cagggcctgg ggagtgatca cccgcacgga
     3841 cataatccct gagagcttta gcagactgcg gtcgcgggtg tatttgttgc cttggaagaa
     3901 tattttctca ggcttgaaaa ctttgccttc cgataccttc agccaggctg tgagcccttg
     3961 ctccagcacc agagagtcga gctctaccct ggcaaagaga aaaccgcggc tgtggcagat
     4021 atccagaatc tggcctatca ctctgtccag ctccttgatg gcaaagcgtt tatcctcaga
     4081 gatcagcagc atctctttga gcctgtcctc ggagaaatag cgcatcccgg ccaggcgcag
     4141 cttcatttga tcggaaggca gcacttccct gaggctaaac ctaagctcga taagcgtgtc
     4201 cgccacggga atgagctccg ggaagggaat tttgacaaag taacggccgc tggagctgag
     4261 atactgctcc agcctcgtta ttgcttctct tatggtagcg ggatcatata ccgcgccgcc
     4321 gataatcccg ctggcctgtt cccaagcggc cgcgtcgaca ggaaaatccg cgtcccagct
     4381 cagcttctga acgctcaagc cgctcaggac gcttagccag aggagcagga acagcggaag
     4441 cgttcgtttc atcgggcagt gctattcccg gtccgccttc agcacagcca ggaaggcttc
     4501 ctggggaacg gtcacgctgc cgatctcctt catctttttc ttgccttcct tctgtttgtc
     4561 cagcagcttg cgtttgcggc tgacgtcgcc gccgtagcat ttggccaaca cgtctttgcg
     4621 catggggttg atggtgctgc gggcaatgat cttggccccg atggaagcct ggagggcgat
     4681 tttaaagaga tgcctgggga tcacttccgc cagggtggcg gtcacgcttt tgccccagtt
     4741 atgggccttg tccgcgtgac agataaagct catggcgtcc actttttcgc cgttgatgag
     4801 gatgtccacc ttcaccacgt tggagctgcg gtattcctta aagtgataat ccagagaggc
     4861 atagccgcgg ctcaccgtct tgagcttgtc ataaaagtcg aagatgatct cgatcaacgg
     4921 catctcatag tggagggcca cgcgcttttc atcgatgtat tggatgtctt tctggatgcc
     4981 gcgccgctcc tgggccagct tcatgatgtt gccgatatag tctgtgggca caatgatttc
     5041 ggtatccata aagggttcct ggatgctgtc tatatagttg ggatcgggaa agtctatggg
     5101 attgttgacc acgatctgct cgccgttttt gaggttgatc agaaatctca cgctgggagt
     5161 ggtggcaatg ataggaatgt tgtattccct cagcatcctt tccttcacga tctccaggtg
     5221 cagcatgccc agaaatccgc agcggaagcc atatcccaaa gcggcggaat tttccttttc
     5281 atataccagc gaggcatcgt tgagcttgag cttggcgata gaatccacca ggttttcata
     5341 gtcctcgcca ttgatgggaa aaatgccgct gaacaccatc ggcttgggct cctgaaagcc
     5401 tggcagggat tcagcgcagc cacctttggc cagagtgatg gtgtcgccca cacgggcgtc
     5461 tgccacctct ttgatattgg cgatgatata tcccgcctca ccggtcacaa gttcctgcat
     5521 gggagcgaat ttgagcccca gatgaccgat ctcctcgatc tcatattccc ggctggtgga
     5581 aaagaggcgg acgcggtcac ccttgcggag tttgccgtcc acgagcctca ccagcaccac
     5641 cacgccgcga tacatgtcaa aataggaatc aaagatcagg gccttgggag gagcgtcttc
     5701 ctttccaacc ggaggaggca gcacttccac caccttgtcc agcagctcgt cgatcccgat
     5761 tccggccttg gcgctcacct tggcgatttc ttcgggaagg cagcccaaaa tgtccgtcaa
     5821 atcgtgctcc gtgccttcaa catcagcttt ggggaggtcg atcttgttga tcgccggcag
     5881 cagttcgagg ttgttttcca gcgccagata gaggttgctc atcgtctggg cttcgatgcc
     5941 ctgagaagca tccaccagca ggatggcacc ttcgcaggac gccaaagcgc gggaaacctc
     6001 atagctgaaa tccacatgcc cgggcgtgtc gatcagattg agcacatagt cctggccctt
     6061 gtagttgtgg atcatgcgga tggcgtggct cttgatggtg atgccttttt cgcgttcgag
     6121 gtccatgctg tccaacacct gggccatatc caccttgccc accacgtggg tggcttccag
     6181 aaaacgatct gccagagtgg atttgccgtg gtcgatatgg gccacgatgc agaaattgcg
     6241 gataaactcc tgtttcattg tatgtccttg atagtcaata agctgtgctg tgcggtatct
     6301 cttcaagagg acaacacccc acaaggtcag cttctgacgg agcgcttatt tgtcaatcaa
     6361 aagggtgaaa gctgtgctgt gagagctggc gctgtgagag ctgatgctgt gagagctgat
     6421 gctgtgagag ctgcgctgtg agagctggcg ctgcgagagc tgcgctgtga gagctggcgc
     6481 tgcgagagcc tcgctgcgag agctggcgct ggggagtgtt gtaacaatcc aggcaaccgc
     6541 caccctaaaa gcctaaaact atggaacttc cattgaactt ccatagggtt ttgtctttat
     6601 tccctccctc ctccctgctt agcattacgg tatcattacg gactcattac ggactaagtc
     6661 cgtaatgagt ccgtaatagg atgcgggaag gaagcagaga ggactgaggc agcttcagct
     6721 aaatggacat ttatcatagc tttatagagt atttggtatt gcggtggctc attactcgga
     6781 tcccctggtc ccagacctgc ctgggatccg atcatcgatc ccgctaagag gatatcacaa
     6841 tcctgaaact tgaaatagct tgacgcgtcg ggctgcgctg aaaacctgct ctatcaacag
     6901 atttgaggta tccatgcatc catacgcgcc agcctgcgga ttatactgcg gagcttgcag
     6961 cagcatgctg ctccacgaaa aagagagcgg attatcccat gccgcgcatt acattcatga
     7021 atacaacgag ggtccctgtc ctggctgcgt caacggtgct caggaatgcg agtttatccg
     7081 ctgcgccaga gaaaaaggtg tgagcggctg cgcggcctgt ccggattttc cttgcgttat
     7141 gatcctgaaa ttcagcaagg acgaatggcc tcaccacatc gaggtgatcg acaacctcat
     7201 gcggatcaga gagataggcg aagctgcctg gctaagtgag caagcagagc ggtggtcctg
     7261 tccggattgc ggtggcagaa cacactggta tcaaaaggct tgcacgatat gcggggcagt
     7321 gatcaccagc gcttatagct aagaaaacca gaagatcagg cctggctacg catccacagc
     7381 ttgatctgcc tcaccagctc ctcggtgtcg tccacgcagc tcagggtggc cctcaggcag
     7441 tttttcagcc tgcgatgggc tcccacgtct ctgagggcga tatcctggtc cttgagatac
     7501 tcaaagagcc ggggcgtcct ctcccccacc gtgaaggtaa gaaagttggt gtgagaagga
     7561 tgcaccgtca agcccggcaa ggctttaagt tcttcataga ctctgtctct gagcttgatc
     7621 acatccagca cctggccgca aaagagcggc acgttttcca gaatggtgag cgcgaacgct
     7681 tgtgagagga tgctggagtg gaaggtagtg aacaccttgc ggaggctgta gatattgtcc
     7741 ggtgaggaga tggcatagcc gaagcgcagt cccgctccgg aaaaggcttt ggagaaactg
     7801 cgggtgagaa tcaggttggg atggctggcc agttcatcca caaagctgga atccgaaaac
     7861 tcgtagtagg tctcgtcgat cagcaccagt ttgtccggca aagcatccac tatctggcgt
     7921 aactgctctt tgggaaagag gttgccgctg gggttgttag ggttgcagag gatggccaga
     7981 ctgcagtccg gatgggaagc cgccttcaga taggcctcgg tgtccaggct gaaatcctcg
     8041 ttcagttcca caaacttgat gcccaggccc accgcctcgc agaaggtctt gtagtcaaaa
     8101 taggaggggg ccgggctcac ggcaaagctg cgctcgccgt ctctcacgct gaggaagata
     8161 tgatagagca tgtcgtccgc gccgttgccc cacaggaggt tttctggctt gataccacag
     8221 cccacatact cagccagctt ctgatgcagt tctcccgtga cgggagacat atagcggttc
     8281 agccgcaccg tctccatcct tttcgaaaac tcgtctttta tcgcctgata ggggtcaaga
     8341 cagctttcat tgaggcacat catccgctgg cggacgggca cgtttacgac cgacggttct
     8401 ttatccagta aatctttacg aaacagtgta ttcatgatta ttctcctttc acagaccata
     8461 gacaagatag gcagaatccg ggagattgca atgccgagcc tggctttctt atttaaaaac
     8521 agaggtacag tgactttaca ggaacagagg atggaacgca gaaaaaggga aaaaagggat
     8581 tggcgggata aatagaacgc agaggacttg atgagaggaa ttacacgatt gattctcaat
     8641 tatcaattct cacttatagt caatcaaatc tgcgtaaatc cctttttttc ctttttccgc
     8701 gtgctgttaa tgctcaattc tctattacac tcgtcatttg attacacatt ctgaagaaaa
     8761 ggcttgacta aaaccaggct tggaagcagg ctaacgcatc atcaagaata gttttggaga
     8821 atctgaccat gcggatacat gagatcctgg accggatcga gagctttgcc cccctggctt
     8881 tggccatgag ctgggacaac tgtggtttgc tgctgggcga accccaggcc gaaatagata
     8941 aagtgtttgt tgcgctggat gttacacccc ggacggtgaa actggcgatc gaagccgggg
     9001 ctcaattgat cctttctcat catcccatca ttttcaggcc tctgaccaaa attacagacc
     9061 cactgctgct ccggttgatc gagcaccgca tagccgtgat cagcctccac accaatctcg
     9121 atgtggcccc cgggggagta aatcacgctc tggcagacgc gttggggctg aaagtgctgg
     9181 agcacctgag ccctgagacg ggagacagct ggaatcatct cagcgtcagc gttccagcca
     9241 ctcacgccgc cagactgaaa gaggccgtct ttgcatctgg agctggattg atcgggcttt
     9301 atgacagctg ttcgactctg caccctgtgc agggtagctt cagggccttg cctggctccc
     9361 agcctttcat cgagggagaa ggacttcagg aagtggacga actggaactg gaattcaggg
     9421 tggatggctc ccggctgggt gccgtcctca aggccatcga aaccacgcat ccctatcaga
     9481 ctcccgcgct ttaccacttt ccggtggcca atcgcaatcc cgcctatggt ttgggcctga
     9541 tctgcgcccc ggcagaacca gctgacctgc caacccttca gaagctctgc acggagcgac
     9601 tgggctgccc ccatcccagg ctctggacgg ctggcaaaga tatctccgcg cccatagagc
     9661 gaatcgccat ctgtggtggc tcgggagggt ctcttatcgc cagcgccagc tccaaagccc
     9721 aggtctatat cagcggtgat tttagctacc acacaatgat ggaatcacgt atccccctca
     9781 tcgatgccgg acacttttac accgagtatc ccgttctggc ggtactggcc aaaaagatgg
     9841 aagacctggg cctgaatagc gtggtgatgc cgatggaaga gtgtgagtgg gt
//
LOCUS       DAOSFN010000093         9816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5889_length_9816_cov_8.442475, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000093 DAOSFN010000000
VERSION     DAOSFN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5889_length_9816_cov_8.442475"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(431..2416)
                     /locus_tag="PLO35_06815"
     CDS             complement(431..2416)
                     /locus_tag="PLO35_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79415.1"
                     /translation="MKKLLVSLALFCTIISLSAQWSQDASTPNAIAAFNAEQVIPKVA
                     ISSDGYCYVCRFDNSGGGYHVYLQYFTPSGLAVWDQPAGILVSANPSMTWLTDYDMDI
                     DQSGNAVIVWQDIRNAGVNNVYIYKVTPEGQLLWFPDGIALSNDSNTEFSNMSPTVFN
                     AGDNSAYVAWQRMQSTASIKLHRISPTGQKLWGEEGITLSPASGSYTWPQIIQGDGDN
                     VLLKYYYDSGPFWAPTRHVYVAKFNPEGVMQWNTALSTAGGISAWQQIIAFEEDGQGG
                     AVVAWYDDRDSNNINDIYIQRVDASGQISMGQNGTLISLNGGAHQFYPRLSVDPLSQR
                     IFAFWKETDASQNNSGVYRQLLDFSGNRLWGDNGEIIQAVGPYPCSPVAAYQTAWGAV
                     CLYEISSQASSDQSLTLKAVCFRASGMSAWDPQTVDLALNPSMKYHYDCAQNRDDWAV
                     LTWEQGSSNMDIYAMRINRDGSLGMQYLPPTDFTAELSPPHTVNLQWQHPSPYWYPLS
                     YQVFMNDELAQTVNGNSNSYSLDLSTPGNYEFKVRAAYDDGYYSPFSDPASVLIVSAD
                     DPLALEMVPVLSVYPNPVRNTAILRLEDIKSGMTARLEVFNLRGQAILSREVRLVSGY
                     NELNLSAGDLSSFPSGVYQIRLKGPSVSIRSRFIVIK"
     gene            2565..3578
                     /locus_tag="PLO35_06820"
     CDS             2565..3578
                     /locus_tag="PLO35_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOH79416.1"
                     /translation="MIAGQNFTTNKGPRLFKKALLAGLAASLLISACGTKEKRMIRDR
                     QWAVEYLRDIPREHYPMALCAAARSQKVERLPREGRKDYKFDKISTSKSVETIKLLAR
                     LGVDLDVRAAGKTPLMLAIEDEFPDIVEALLKSGANPLIKDPGGKSASDYAQVSQNQE
                     IIKLLDDALTNIYSEYYEISDPHRRALLDSLYNKRPIARADTSKVDTLITEIVAETQA
                     FHYAEASQSSIAPAKKRINLGGVSNYSDYDEAPKLQSPVRPLYPPTALEDGIQGSVLL
                     EVEVLPTGRVGEVKVIRGVQSGSGGIDEAAVSALKSAHFSPARKAGYPVQTAIQISVK
                     FKI"
     gene            complement(3609..6059)
                     /locus_tag="PLO35_06825"
     CDS             complement(3609..6059)
                     /locus_tag="PLO35_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOH79417.1"
                     /translation="MQFIDIQRFLPLVSKPSRYIDHELNAVNRDPRNFQAKFCFAFPD
                     VYEVGISHLGLKILYSIINRRDDAMADRVYLPWLDLLEILRAEDLPLFGLESGLAIRD
                     FDVLGITLQSELTYSNVLELLAVSQIPILSQQRGPGHPIVLAGGPCTVNPLPLKDFID
                     VFFIGEAEEAIGEIAEVIATIESRTERLKALSSISGCYVPLYHDPILARDKGFKIKAR
                     KYAAFHSSEHQHSPQLLSWQLATHNRYVAEIMRGCSRGCRFCQAGFIYRPVRERSATD
                     ILEQLLKEVRLSGWDEVGLMSLSSSDYTCVKELLFLLLNALDQQKTHVSLPSLRVDSL
                     DADLVGLMKELGREGLTIAPEAGSQRLRDVINKNLTEEEITRGVQIAQELGWQRIKLY
                     FMLGLPTETDADIDAITELIGKVNSLVRGRLQINVTLSPFIPKPFTPFQRCGFLTPSV
                     LLERALRIKKSFGRNRNIKIKYHTIENSLLEAILARGDERVAKLIHSAWQSGARFDGW
                     NECFDFTCWQAALDAQTLNYEDLTAPIPGDVSLPWSFIDYGVCESWLEQEYQKALSAQ
                     TTEDCRDLCSVCGVCDQGLRTMDAPRPDKVILPPKSTDKARPHSGDGRQRYRVFYRKE
                     GVLRFISHLDWMRMLFRFISKADLDTVFTQGFSPHPKVSLSPPLPLGMESQVEYFDVS
                     CYQAHSTERIMDVYKAIQLPGFELTGVELISGKGTIPSGETLHFEAPEALLPKVLTAC
                     SAYAASSAWPYTKTVELRSKDYDLKEIVTQLSLVDKTLSIKKTLIGPSVWDILAELTH
                     SPKANLFAGRLIRVDWIW"
     gene            complement(6068..6799)
                     /locus_tag="PLO35_06830"
     CDS             complement(6068..6799)
                     /locus_tag="PLO35_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOH79418.1"
                     /translation="MKIGGFQKFSLLDYPGHLAAIVFTRGCNFRCPYCHNPELVDPNR
                     YGELIPEQEVMDFLISRQGKLGAVSITGGEPTLQEDLIGFLRKLRSMKYLIKIDTNGS
                     RPEVLEEISREGLVDYWAMDIKAPPHLYKLVTGYDLDFEQIIASMDILRASGKDYEFR
                     TTFFDALFNWSDLSLISALLKPGDHFYLQQCRYDNTLDGVQSSGLAKHSKDGSEYFHL
                     VEHPACQNLLEWGGRNRVNVGIRSL"
     gene            complement(6796..8925)
                     /locus_tag="PLO35_06835"
     CDS             complement(6796..8925)
                     /locus_tag="PLO35_06835"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOH79419.1"
                     /translation="MFSMIRKRSGNIVAFDKAKIVRAIQKAGEATGEFSEDIAEVLTL
                     RVLNLAIQVINSDIPEVERVQDIVEEVLLASPYKRSAKAYIIYRDQHARIRELVSTAG
                     VRLIDQYLEKLDWQVNENSNMAYSLQGLNNYIAAEVSKTYWLNKIYPPEIRAAHVDGD
                     IHIHDLGQLSVYCVGWDLMDLLLTGFCGAEGKVESAPARHFRSALGQIVNFFYTLQGE
                     AAGAQAFSSFDTLLAPFIYYDKLSYDDVKQALQEFVFNINVPTRVGFQTPFTNITIDL
                     IPPTIYKDQPVIIGGQPQDRTYKDFAAEMDLLNRAFLEVMIEGDAKGRVFTFPIPTYN
                     ITKDFDWEDPKLDYLWEATAKYGIPYFSNFVNSDMDPNDARSMCCRLRLDTRKLEARG
                     GGLFGANPLTGSIGVVTLNLPRIGHLARSEEEFFQRLEGILVIAKNSLEIKRKILETY
                     TSGGLYPYTKFYLKSIRERFSQYWKNHFSTIGIIGMNEACENFLGVNIGADKGRAFAV
                     KVLDFLRERLITIQEETHNNYNLEATPAEGTSYRLAILDKKFFPDIQSANTQGDTPFY
                     TNSTQLPVNYSDDIFEVLDLQDELQTKYTGGTVLHIFGGERVEHGRSIRKLVHTICSN
                     YHLPYFTFSPTFSICPQHGYLVGEQADCPTCHRSTEVFSRIVGYLRPVSQWNEGKKAE
                     FRLRTTFDTLKHHEPSARIQTGEIKLP"
     gene            complement(9270..>9816)
                     /locus_tag="PLO35_06840"
     CDS             complement(9270..>9816)
                     /locus_tag="PLO35_06840"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOH79420.1"
                     /translation="ERIFSDKLTIRQQPRDPELASSTAFDNEGTPTQELYLLKNGVFM
                     AIPTDLNYAHKLGLEPTGNGFRPEVEYMPGPRPFNTSLQTGDMSLDAMIASIDKGLIV
                     HSLMGAHSGNILNGDYSVGVSSGFLIENGKIVARVKDCMLSGNVYETLNNVIAIGSEA
                     TALGSSKLPALLCDGVSVAGK"
BASE COUNT         2287 a         2534 c         2689 g         2306 t
ORIGIN      
        1 ctaatccacg ccgccagcgt tcgtcctgag ccaggatcaa actctccatc attataataa
       61 tgattgtaaa ttgacaagag cttttcctct cattcccact ctataaactt gtaaagatct
      121 ctgtgtcccg aaaattcaca ttgtcgtgaa cacgtttcgt catcttttca aaggggcttt
      181 ttttgtcaag ataaaaagtg atgttttctc acgtttttcc acgatggatt actaactagc
      241 tgcaaatatg acttatacga tttgaacttt ttctggggct tacagcgttg atgctccctc
      301 tatatcgctg ggcgctcccc tttgggcatc cctcacctct ccttcgtgtc tcccgtgccg
      361 aggcaaagga gaggtaaagg agaggcatgg gtgatgcaaa ggaggctaat gggaaagcac
      421 cccggcactg ctatttgatg acaatgaacc tggaccggat ggagacagac ggccctttca
      481 gcctgatctg ataaaccccg ctgggaaaac tggacaggtc cccggcactc aaattcagtt
      541 cattatagcc ggaaacgagc ctcacttccc tgctcaggat ggcttgtccg cgcaggttga
      601 acacctcgag ccgggcggtc ataccgctct tgatgtcttc cagcctcagg atcgctgtat
      661 tcctgaccgg gttgggataa actgaaagaa cgggcaccat ttccagggcg aggggatcgt
      721 ccgcggaaac gatcaaaacg ctggccggat cgctaaaggg agagtagtat ccatcgtcat
      781 aggccgctct gaccttaaac tcatagttcc ccggggtgct gagatccagg ctgtagctgt
      841 tggagttccc attcactgtc tgggccagct cgtcgttcat aaacacctgg taagataagg
      901 gataccagta gggagaagga tgctgccact gcagattgac ggtgtgtgga gggctcagct
      961 cagcggtgaa atcggtgggt ggaaggtact gcatgcccag gctgccgtcc cggttgatcc
     1021 gcatggcata gatatccatg tttgacgatc cctgctccca ggtcaggacg gcccagtcat
     1081 cgcggttttg ggcgcagtca taatgatact tcatgctggg gttcagggcc aggtccacgg
     1141 tttgagggtc ccaggcagac attccgctgg cccgaaaaca gacggccttg agggtaagtg
     1201 actgatcgga agaagcttga gagctgatct catagaggca cacagctccc caggcagtct
     1261 gatatgccgc cacaggagaa cagggataag gccccactgc ctggatgatc tcaccattat
     1321 caccccagag acggtttccg ctaaagtcca atagctgacg atagacgccg gaattgtttt
     1381 ggctggcatc ggtttctttc cagaaggcaa agatcctctg gctgagcgga tcgacactga
     1441 gcctgggata aaactggtga gcacccccgt tgagactgat gagagtgccg ttttggccca
     1501 tgctgatttg tccgctggca tcgacccgct ggatatagat atcattaata ttgttactat
     1561 cccggtcatc ataccaggct acgactgcac ccccctgccc atcttcttca aaggcgatga
     1621 tttgttgcca ggcgctgatg cctcccgcgg tactgagagc ggtgttccac tgcatcactc
     1681 cctccggatt gaacttggcg acatagacat gacgggtggg ggcccaaaag ggaccgctgt
     1741 cataatagta tttgagcaga acgttgtcgc cgtctccctg gatgatttgt ggccaggtgt
     1801 aggaacctga ggcggggctc aatgtgatgc cctcttctcc ccagagtttc tggcctgtgg
     1861 gactgatcct gtgaagcttg atcgacgcag tgctctgcat ccgctgccag gctacgtaag
     1921 cgctgttgtc tccagcgttg aagacggttg gcgacatgtt ggaaaattcc gtattgctgt
     1981 cattagagag ggcaatgcca tccggaaacc acagcaactg cccctcagga gtaactttat
     2041 agatgtaaac gttgttgact cccgcgttgc ggatatcctg ccagacgatg acggcgttgc
     2101 cggactggtc tatatccata tcgtaatcgg tgagccaggt catggaggga ttggcactca
     2161 cgaggatgcc ggccggctga tcccagaccg ccagtccact gggagtaaag tattgcaggt
     2221 aaacgtgata tccaccccca gaattgtcaa aacggcacac atagcaataa ccatctgagc
     2281 tgatggccac tttggggatg acctgttccg cgttgaaagc cgcgatggca ttgggagtgg
     2341 aggcatcctg actccattgt gcggacagtg aaataatggt acaaaaaagg gcgagcgaga
     2401 caagcagctt tttcatgaca tacctctggt ctgtttgatt ggtgttacaa atagttagct
     2461 ggcataatcg gtcaagttat ttttcaagtc aaaatccgcc ttcgatgaat ttccgcttga
     2521 catccagcca aggcctcccg cagaggttac acacaggagt tttaatgatc gctggacaga
     2581 atttcactac caacaaaggc ccaaggctat tcaagaaggc attgctggca ggcctggccg
     2641 cctccctgct gataagtgcc tgcggcacta aagaaaagcg tatgatcaga gatcgtcagt
     2701 gggcggtcga gtatctgcgt gacatccccc gggagcatta tcccatggcc ctttgcgcgg
     2761 cggcccggtc tcaaaaggtc gaacgcttgc cacgggaagg caggaaggac tataagttcg
     2821 ataagatcag cacttccaag agtgtggaga cgatcaagct gctggcccgt ttgggagtgg
     2881 acctcgatgt gagggcggct ggcaagactc ccttgatgct ggccatagag gacgaattcc
     2941 ctgatatcgt ggaagccctg ttgaaatctg gggccaatcc tctgatcaaa gacccggggg
     3001 gaaaaagcgc ttctgattat gcgcaagtga gccagaacca agagattatc aaactcctgg
     3061 atgacgcgtt gaccaatatc tacagcgagt attacgagat ttccgatcct caccgcaggg
     3121 cgctgcttga cagtctttac aacaaacggc ctatcgcgag agctgacaca agcaaagtgg
     3181 atactttgat cacggaaatc gtggccgaaa cccaggcgtt tcattatgcc gaggccagcc
     3241 agagcagcat cgctcccgct aaaaagagaa ttaacctagg cggggtaagt aattacagcg
     3301 attacgacga agctccgaaa ctgcaaagcc cggtcagacc actctatcct cctactgctt
     3361 tggaggatgg cattcaggga tcggtgctgt tggaagtgga agtcttaccc accggacggg
     3421 taggggaagt gaaggtgatc agaggagtgc agtctggcag cggtggcatc gatgaagctg
     3481 ctgtcagcgc gctcaagtct gcccatttct cccctgccag gaaagcgggg tatccggtcc
     3541 agacagctat tcagatctcc gttaagttca agatataggg cccgggatcg cgatcctgga
     3601 tagcgggtct accaaatcca gtcaacgcgg atcaggcgtc ccgcgaacaa gtttgccttg
     3661 gggctgtggg tcaactccgc cagaatatcc caaaccgagg ggcctatcaa ggtctttttg
     3721 atcgataggg tcttgtccac aaggctgagc tgagtgacga tctctttgag atcatagtct
     3781 ttagagcgca gctccaccgt cttggtatag ggccaggcac tgctggcggc gtatgcgctg
     3841 caggcggtca ggaccttggg aagcagggct tcaggtgctt caaaatggag cgtttctccg
     3901 ctggggatcg tcccttttcc agagataagt tccaccccgg tcagctcgaa gcccggaagc
     3961 tgaatggctt tgtaaacgtc cataattcgt tcagtgctat gagcttgata gcatgagacg
     4021 tcaaagtatt ccacctgact ctccatcccc aaagggaggg gtggcgacag actgaccttg
     4081 ggatggggac tgaaaccctg agtgaatacg gtgtccagat cggctttgga gataaagcgg
     4141 aagagcatcc gcatccagtc cagatgagat atgaaacgca ggacaccctc tttgcgatag
     4201 aacacccgat agcgttgacg tccgtctccc gaatggggcc tggccttgtc ggtgcttttt
     4261 ggcggcagaa tgactttgtc cggcctgggt gcatccatgg tgcgcagtcc ctgatcacag
     4321 actccgcaga ccgagcacag atcacggcaa tcttctgtgg tctgtgcact tagggctttc
     4381 tggtattcct gctcgagcca gctctcgcag acgccgtaat cgatgaagct ccagggcagg
     4441 gaaacgtccc cggggattgg agctgtaagg tcctcatagt ttagagtctg cgcatccaga
     4501 gcagcttgcc agcaggtaaa atcgaagcat tcattccagc cgtcaaagcg agcaccggat
     4561 tgccaggcac tgtgtataag cttggcaacg cgttcatccc ctcgcgccag aatggcttcc
     4621 aggagggagt tttcgatggt gtgatacttg atcttgatat tgcggttgcg gccaaagctc
     4681 tttttgatcc tcagagcccg ctccagcaga acagaaggcg tgagaaatcc acagcgctga
     4741 aagggagtga agggtttggg gataaagggt gaaagggtca cgttgatctg caggcgtcct
     4801 ctgaccagtg aattgacttt accgatcaat tccgtgatgg catcgatatc ggcatccgtc
     4861 tctgtgggca gaccgagcat gaaatagagc tttatccgtt gccaaccgag ttcctgggca
     4921 atttggaccc cccgtgtgat ctcctcttcc gtgaggtttt tgttgatcac gtcgcgtaga
     4981 cgctgtgagc ccgcttccgg agcaatggtg agaccttcgc gccccagctc tttcatcaag
     5041 cccaccaggt cagcgtccag gctgtcaact ctcagagaag gaagggatac atgcgtcttc
     5101 tgctggtcca aggcgtttaa gagaaggaag agcagttcct tcacacaggt gtaatcgctg
     5161 gaggaaagcg acatcaaacc cacttcatcc caaccgctga gccttacttc cttcagaagc
     5221 tgctccagga tgtctgtggc cgagcgttcc ctgacgggac gataaatgaa gcctgcctga
     5281 cagaaacggc agcctctgga acagccgcgc atgatctctg ccacatagcg gttgtgggtg
     5341 gctagctgcc aggaaagcag ttgcggactg tgctgatgct cggagctgtg gaaggcagcg
     5401 tattttctgg ctttgatctt aaatccctta tctctggcca gaatgggatc gtgatagagc
     5461 ggtacgtagc aacctgagat ggagctaagc gctttcaggc gttctgttct gctctctatg
     5521 gtggcgatca cttccgcgat ctcaccgatc gcttcttccg cttccccgat aaagaagaca
     5581 tcgataaagt ccttcagagg caatggattg accgtacagg gtcctccagc caggacgatc
     5641 ggatgcccgg ggccacgctg ctgggacagg atgggaatct gtgacactgc aagcagctcc
     5701 agcacgttgc tgtaggtgag ctcgctctgg agcgtgatgc ccagcacgtc gaaatccctg
     5761 atcgccagtc cgctctccag tccaaacaaa ggcaggtctt cagccctcag tatctccagc
     5821 aggtccagcc agggaagata tacccggtcc gccatggcat cgtctcgtcg gttgataatg
     5881 ctgtagagga tttttagtcc gagatgggat atccccacct catagacgtc tggaaaggca
     5941 aaacagaatt tggcctggaa gttccgcggg tccctattga ctgcgttaag ctcgtgatct
     6001 atgtagcggg agggcttgga aacgagcggc agaaaccttt gtatgtctat aaattgcatt
     6061 gatgtactta aagtgacctg atacctacgt tgacccggtt gcgtccaccc cattccagga
     6121 ggttctgaca ggcaggatgc tccaccagat gaaagtattc cgagccgtct ttgctgtgtt
     6181 ttgccaggcc ggaactctgc acgccgtcca gggtattgtc atatcggcac tgctgcagat
     6241 agaagtggtc gccgggcttg agcagcgcgc tgatcaggct caggtcgctc cagttgaaca
     6301 gcgcgtcaaa gaaggtggta cgaaactcat aatccttgcc gctggccctg agaatatcca
     6361 tgctggcgat gatctgctca aaatccagat cgtagccagt gaccagcttg tagagatgag
     6421 gcggggcttt gatgtccatc gcccagtaat ccaccaggcc ctctctggaa atctcctcca
     6481 gtacctctgg acgggagccg ttggtgtcta tcttgatcag atacttcatg cttctcagct
     6541 tcctgagaaa gccgatcagg tcttcctgaa gagttggttc cccgcctgtg atggacacgg
     6601 cgcccagttt gccctgccgg ctgatcagaa agtccataac ttcctgctcc ggtatcagtt
     6661 caccatagcg gtttggatcc accagctcag gattgtggca atagggacag cgaaagttgc
     6721 agccccgcgt gaaaacgatg gcagccagat ggccagggta atccagcagg gaaaacttct
     6781 ggaaaccgcc gatcttcatg gaagcttgat ttctccagtc tgaatcctgg cagagggctc
     6841 atgatgcttg agagtgtcga aagtggtgcg cagcctgaac tcggcttttt tgccttcgtt
     6901 ccattgcgag accggccgca ggtatcccac aatgcgggaa aacacttcgg tggacctgtg
     6961 gcaggtgggg caatcggctt gttcgccaac cagatagcca tgctgagggc agatgctgaa
     7021 ggtgggtgaa aaggtgaaat atggcaggtg gtagttggag cagatggtgt gcaccagttt
     7081 gcggatgctg cgtccatgct caactctctc tccaccaaag atatgcagca ccgtgccacc
     7141 ggtatacttg gtctgcagct cgtcctgaag gtccagcact tcaaagatat cgtcagagta
     7201 gttcaccggc aactgagtgc tgttggtata gaagggtgtg tcaccctgag tgttggcgct
     7261 ttggatatca ggaaagaact tcttgtccag gatcgccagc cggtagctgg tcccctcggc
     7321 gggagtggcc tcgagattgt agttgttgtg ggtctcttcc tggatggtga tgaggcgttc
     7381 acgcaggaaa tccagcactt tgacggcaaa agctctgccc ttatcggccc cgatattcac
     7441 acccagaaag ttctcacatg cttcgttcat gccgatgatc ccaatagtgg aaaagtggtt
     7501 cttccaatac tgggaaaagc gttcgcggat gctcttgagg tagaactttg tgtagggata
     7561 aagtcctcca ctggtgtagg tttccaggat cttgcgcttg atctcgaggc tgtttttggc
     7621 aatgacgaga atgccttcga ggcgctggaa aaactcctct tcactccggg ccagatgacc
     7681 tatcctgggc aggttcaggg tcaccacccc aatcgagccg gtgaggggat tagcgccaaa
     7741 gagacctccg cctctggctt ccagcttgcg ggtatcaaga cgcaggcggc aacacatgga
     7801 gcgggcatcg ttggggtcca tatcagaatt tacaaagttg gaaaaatacg gaatgccata
     7861 cttggctgtg gcttcccaga ggtaatccag tttgggatct tcccagtcga agtccttggt
     7921 gatattgtag gtagggatcg ggaaggtaaa gacgcggcct ttggcgtcgc cctcgatcat
     7981 gacctcgagg aaagcgcggt ttagcaggtc catctcagcg gcgaaatcct tgtaggtcct
     8041 atcctgaggc tgcccgccga tgatcacagg ctggtctttg tagatagtgg gaggaatgag
     8101 atcgatggtg atgttggtga aaggcgtctg aaatcccacc cgggtgggaa cgttgatatt
     8161 gaagacaaac tcctgcagag cttgcttgac atcatcgtaa ctcagcttgt cataatagat
     8221 aaagggcgcc agcagggtat caaaactgga aaatgcctga gcgccggccg cttcaccctg
     8281 gagtgtgtaa aagaaattga cgatctgccc cagcgcgctt cgaaaatgac gggcgggagc
     8341 gctttccacc ttgccctcgg caccacagaa acccgtgagc aggaggtcca taaggtccca
     8401 gcccacgcaa taaactgaaa gctggcccag atcgtggata tggatatcgc catccacatg
     8461 cgcggccctg atctcgggag ggtagatctt attgagccag taagttttgc tcacttccgc
     8521 ggcgatatag ttattcagcc cctgcagaga ataggccata ttggaatttt cgttcacctg
     8581 ccaatccagc ttttccagat actggtcgat cagcctcact ccagccgtgg aaaccagttc
     8641 gcggatacgg gcatgctgat cgcggtagat aatataggcc ttggcggagc gtttatacgg
     8701 cgaggccagc agcacttctt ccacaatatc ctgaacgcgt tcaacctccg ggatgtcgga
     8761 gtttatcacc tggatcgcca aattcaacac cctgagcgtc aaaacctccg cgatatcctc
     8821 tgaaaactcc ccggtcgctt caccggcctt ttggatagcg cggacgatct tggccttgtc
     8881 aaaagccaca atattcccgc tgcgtttccg aatcatcgaa aacatcgcaa tcctccctta
     8941 aactctatat ttatattttg ttatttatca gtccattaca cgcatacaac tcactttaac
     9001 taagtggtaa acagcgttgc tactcgcatt tatttagaca cttagcaatt ttgtcaatac
     9061 taaatcatca ctattttgcg aaatcctgtc ttgaagagag gaaggacatc cacagcggtc
     9121 acttagatgg ctgagatgaa atggatttga ggatggaatg tacagccgtt caccagacaa
     9181 gccgcaaccc actgccacac aaggcaccat aagaactacc ccggaagcat gtaaactccc
     9241 ggggtagctt tcaactgatt gattaaggtc tatttgccgg ccacgctgac cccatcgcag
     9301 agcagcgcag gcagtttgga agagcccagc gccgtcgctt ccgatcctat ggcaatcacg
     9361 ttgttgaggg tctcatagac attgccggag agcatgcagt ccttgactct ggccacgatt
     9421 ttgccgttct cgatcaggaa tccactcgac acgcccacgg aataatcccc attgaggatg
     9481 tttccagagt gagcgcccat cagcgaatgc acgatcaggc ctttatctat ggaagcgatc
     9541 atggcatcca ggctcatgtc tccggtttgc aggctggtat tgaaaggcct gggaccgggc
     9601 atatattcca cttccggtct gaagccgttc ccggtgggct cgaggcccag cttatgagcg
     9661 tagttcagat cggtggggat ggccatgaaa acaccgtttt tcaacaaata gagttcctga
     9721 gtgggagtgc cttcattgtc gaatgctgtg gagcttgcca gttccgggtc cctgggctgc
     9781 tgccgaatag tgagtttatc gctgaagatc ctctcc
//
LOCUS       DAOSFN010000094         9676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_5997_length_9676_cov_8.865710, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000094 DAOSFN010000000
VERSION     DAOSFN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5997_length_9676_cov_8.865710"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1446)
                     /locus_tag="PLO35_06845"
     CDS             complement(250..1446)
                     /locus_tag="PLO35_06845"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOH79421.1"
                     /translation="MKILVINCGSSSLKYEIYEMPQQLSLGKGQVERIGQPLGCIKQD
                     FEDRHFYREQPVPSHREAFELMMRALLDPESGILRSVDEISGIGHRVVHGGEQYSASV
                     VIDADVTRAIELNCELAPLHNPANLTGIRESQAIFLAQPQVAVFDTAFHQTLPPSAYL
                     YGIPREFYEKYRIRRYGFHGTSHRYVAGVALQMMKRSAENTNMITCHLGNGASITAIQ
                     QGRSIDTSMGFTPLEGLMMGTRSGDLDPSIIFYLEERGYDFHELNNILNKKSGLLGLS
                     GISSDLRDLEAASEAGDANAGEALDTYAYRIRRYIGAYAANLIKVDIIVFTGGIGQYG
                     VKMRDRICDRLENIGIHIDPELNQKVGHQPGIISKPYSPIAILVIPTNEELQIAIDTF
                     GLVQQP"
     gene            complement(1603..2043)
                     /locus_tag="PLO35_06850"
     CDS             complement(1603..2043)
                     /locus_tag="PLO35_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOH79422.1"
                     /translation="MNDKIYTNLIGIQREMLKLLGEVSTLTSSPLAIEDAIDDYWHPK
                     CDVYQTDTEWVIVVELAGINKDEVSISLTDEYLRVSGIRNFPSPSCATSYYSMEIETG
                     RFDRRVFFPDIQIDRDNPRVQYVNGILRIAFSLSPVLERIIPIE"
     gene            complement(2056..3402)
                     /locus_tag="PLO35_06855"
     CDS             complement(2056..3402)
                     /locus_tag="PLO35_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOH79423.1"
                     /translation="MKHVILISAILMVLSASLVAEDWLTIYNDDLSLVRSRFELELEA
                     GRQSYNYDDITSRIIAPSVIVTSTEPGFTVAEQNYEYDLAGKWQIMAKYLDKEVVLLT
                     KDNSRVRGVLKFFDGTSYGVTENGTGRLIVVSDSETQMVQLAKLPDNFYVKPTLHWGL
                     ISPKAGKIPIQLTYMTGGFSWDVTYNSVWDDKALALNSWVTINNTSGKAFEDVTLKLI
                     AGDVNRVYESYDKYSRYEYGMVEDAMAVAEAAPAFEEKAFHDFHMYTLDQKLSFANNQ
                     TKQIALYPTQNVKAEGTYEYTTWGDGVSSIIKFKNTEENGIGKPLPKGTIKVYKQDSD
                     GNLEFIGEDSIDHTSRNEEIKIRTGKAFDLVASTLTKDQANLGRDVSERTIQITLRNN
                     SKEKKTIQVIHYMGGNTKLVQKDIEPEIDTNNKATFLVEIEPDAQRIFSFRERTEY"
     gene            complement(3448..4797)
                     /locus_tag="PLO35_06860"
     CDS             complement(3448..4797)
                     /locus_tag="PLO35_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOH79424.1"
                     /translation="MPKRVFLAALLMFIALALRGENWLTIYNEDLSLVRSSFELNLMT
                     GRQDYNFDDIPHRIMPASVIVSSQAEGFQVAEQNYEYDLAGKVEILRKYLNSEVEITT
                     KDGSRIRGILKFSDYSSYGIQDDYDGRLYLVSAAETQMIQLASLPENFYTRPTLRWSL
                     IAPQAGKYPLQLSYLTGGLSWDVTYNGVWNDSTLALNSWVTIKNTSGKAFEDTNLKLI
                     AGDVNVVKQLHIRGGRANEINYSIDGMSATGGAPSFAEKSFHDYHMYSLDQKLSFANN
                     QTKQLALYPRMDIKAEGIYEYPTWSGAVNSIIQFKNTEANGIGKPLPKGSLKIYREDT
                     DGNLEFIGEDSIDHTGRNEEVKITTGKAFDLVASTLSKDQTNVSKKVTERTVQVTLRN
                     NSSQTRIIRVVHQMSGNTRIIQHDLTPEIDSDNKATFSVEVKADAQVIFSFRERSES"
     gene            5122..6528
                     /locus_tag="PLO35_06865"
     CDS             5122..6528
                     /locus_tag="PLO35_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOH79425.1"
                     /translation="MENIRIGIVGNIGVGKSTFIEAASSAPLNKVLTSVYPKPNGTEG
                     VYAFPEKFNPIVLDSFYKDPVANAFMAQIEFFNGRLDRQKLIQTCRGIVLEDRTLAED
                     YHIFGKAQRVLQNMSEPEFIAYQRTFRLMTEQIKEPDLLVYFKAEVPILQERIRERGR
                     ESELAISADYLELLNNLYEDFIAHHVHCPVLVIDADRAVEKTEWLRRTVNLVAEQIKA
                     LELRVSSPGISEWVTLPQTEATLKAIDVERQLEQYLAEHQKLITIAGNVGLGKSTLTA
                     IMGRSLKINTLYERPEENPLLEKFLGDKKTWCYDLQMHFLQMRAQQRRLGKSGDGSYV
                     KDRSLPEDLLVFCNQFHADGLLTDDELDNLITQFQTVNRQLPSSDLIILLHGSPSLAW
                     ERIQQRGREMELEGGWQFTEINNLNHWYKAYGSNVVKLGFHNGPVLEIDVEKLDLTNR
                     IHVGYIFERVLETLKAQD"
     gene            7057..9549
                     /gene="leuS"
                     /locus_tag="PLO35_06870"
     CDS             7057..9549
                     /gene="leuS"
                     /locus_tag="PLO35_06870"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOH79426.1"
                     /translation="MEYPFNEIEPKWQQIWRERRVFSAPDLADQPKYYVLSMFPYPSG
                     VLHIGHASNYSIGDAVTRLKMMEGYAVMQPMGYDSFGMPAENYAIEHNSHPRLTTEEN
                     IAAMRTQFDAMGFGLDWEREVSTCRPDYYRWGQYLFKKLYEKGLVYRKRSFQNWCDAC
                     QTVLANEQVEDGHCWRCHADVRQKELEQWYFRITDYAEELLDFSKMIDWPERVIQMQK
                     HWIGKSEGTRIDFVLEEDNSPISVFTTRPDTIYGVTFMALPPEHPLVQRWLAAEPDNH
                     AMRAFCDKVINEDKISRSAADTIKEGVFSGRWCVNPLNGNRVQIWITNYVLMDYGTGA
                     VMAVPAHDQRDYEFAKKYDIPLIIVIQKPDHSLAIENMTEAYIEPGVMVNSAPFDGLD
                     SEAAKSAISRLIEEKGWGEVTATYRLRDWGISRQRYWGNPIPVIHCPICGVVLVPDKD
                     LPVLLPDNVQVGKTTSNPLLSVPEWLHVKCPQCGADSQRETDTMDTFVDSSWYYARYA
                     DPKNTAAPFDPAKANYWLPVDQYIGGIEHACMHLLYARFIGKFMRDLGWLHCDEPFSR
                     LLTQGMVLKDGAKMSKSKGNVVDPTYIVERFGADTLRVYLLFASPPDKDVEWSDDGIM
                     GAFRFLNRVWRLINANLDIIRKGLQIWPSDEELDQLDPAIKNLLFSTHHTTKKWLDDC
                     LQRMQYNTAIAAIMEHLNHCVGVKDPQKLEGAALAVYAEACGIVPQLLYPFAPHIAEE
                     LWQLMGMEALLHASGLPGYEERWLTRDLVTYVVQVNGKLRGKLEVSPSTDDNSLKTMA
                     LELENVQRALEGLSVKKVIVIPGKMVSIAAGK"
BASE COUNT         2079 a         2635 c         2750 g         2212 t
ORIGIN      
        1 aattattgat aggagcacgc ggatctatct aaactaccgt cccgatggga ctcaatgcgc
       61 catttgttca caagctctca agccaatgtt gcctgccatc cattgttctt gctgcgttac
      121 atcacgaccg tctcccgggc cccactcacg cataacccac cccaaagcac gggataagcg
      181 tgcgataagc gtgcgataag cacgggatag tgaagggtag gcttagccgg gtaacgcgtc
      241 aggccatcgt tacggctgct ggaccaatcc aaaggtatcg atcgcgatct gcagttcttc
      301 gttggtgggg atcaccagga tggcgatggg ggaatagggt ttggagatga tgccgggctg
      361 gtggcccacc ttttgattga gctcgggatc gatgtggatg ccgatgtttt ccaggcgatc
      421 gcagatgcgg tcgcgcattt tgacgccata ctgtccaatc cctccggtga atacgataat
      481 atccactttg atgaggttgg cggcgtaggc cccaatgtat ctgcggatgc ggtaggcata
      541 ggtgtcgagg gcttcgccgg cgttggcgtc tccagcttcg gaggcagctt cgaggtctcg
      601 cagatcgctg gaaattccag acaggcccag cagtccgctc ttcttattga ggatgttgtt
      661 gagctcgtga aagtcgtagc cgcgctcttc cagatagaag atgatggagg ggtccaggtc
      721 tccggatctg gtgcccatca tgagtccttc caaaggggtg aagcccatgg aggtatcgat
      781 gctgcggcct tgctggatgg cggtaatgga agcgccgttg cccaggtggc aggtgatcat
      841 gttggtgttt tcggcagagc gtttcatcat ttgcagcgcc acacctgcca catagcgatg
      901 ggaggtgccg tggaaaccat agcggcggat gcggtatttc tcgtaaaact ctctggggat
      961 gccatataga taggcactgg gaggcagggt ctgatgaaaa gcggtgtcga aaactgccac
     1021 ttgcggctgg gccagaaaga tggcctggct ttcccggatg cctgtgaggt tggcgggatt
     1081 gtgcagcggg gcgagctcgc agttgagttc gatggcccgg gtcacatcgg cgtcgatgac
     1141 cacggaagcg gaatattgct ctcctccatg caccacgcgg tgtccgatcc cgctgatttc
     1201 atccaccgac ctgaggatgc cgctctcggg gtcgagcagc gctctcatca tcagttcaaa
     1261 ggcttctcga tggctgggaa cgggttgttc ccgatagaaa tgccggtctt caaaatcctg
     1321 tttgatgcag ccgaggggct gtccgatacg ctccacctgg cctttgccga ggctgagctg
     1381 ctgaggcatc tcgtagatct catacttgag gctggagctg ccgcagttga tcactaagat
     1441 cttcatgcgc tggagtgtcc tttatgtgta tatttttgtt atgcgttatg cgtgaagcgg
     1501 gcagacaggc cacgcaccgg ctaaagcctg tgttacgacc ggctaaagcc agtgttacca
     1561 gcgttatgcg ttgcgcttaa cgcttaacgc gtcacgcctg actcactcga tcgggatgat
     1621 cctctccaat accgggctca gcgagaaggc gatcctgagg atgccgttga cgtattgcac
     1681 cctgggatta tcgcggtcta tctggatgtc cggaaagaag acgcggcggt caaagcgtcc
     1741 cgtctcgatc tccatgctgt aatagctggt ggcacagctg gggctgggga agttgcggat
     1801 tccgctcacg cgtaaatact catccgtgag agagatgctc acctcgtcct tgttgatgcc
     1861 ggccagctcg accacgatca cccattccgt atccgtctga taaacgtcgc atttggggtg
     1921 ccagtagtcg tcgatggcgt cttctatggc gaggggactg ctggtgaggg tagatacctc
     1981 tcccagcagt ttgagcatct cgcgttggat gccgatgaga ttggtgtaaa tcttatcgtt
     2041 catagctcta aagctttagt attcagtgcg ttcgcggaag ctaaagatgc gctgggcatc
     2101 gggctcgatt tccaccagga aggtggcttt gttgttggtg tcaatctcgg gttcgatgtc
     2161 tttttgcacg agtttggtgt taccgcccat gtagtggatc acctggatgg tctttttctc
     2221 tttgctgttg ttgcgcaggg tgatctggat ggtgcgttcg gacacgtccc gtcccagatt
     2281 ggcctggtct ttggtgaggg tgctggccac gaggtcaaag gccttgccgg tcctgatctt
     2341 gatctcttcg ttgcgggagg tatggtcgat agagtcttcg ccgatgaatt ccagattgcc
     2401 gtcgctatcc tgtttataga cctttatggt acccttgggg aggggtttgc cgatgccgtt
     2461 ctcttcggta ttcttaaact tgatgatgct gctgacgcca tcgccccagg tggtgtactc
     2521 ataggtgcct tcggctttta cattctgagt ggggtagagg gcgatctgct tggtctggtt
     2581 gttggcaaag ctcagcttct ggtccagggt gtacatatga aagtcgtgaa aggccttttc
     2641 ttcaaaggca ggagctgctt cggcgacggc catcgcgtct tccaccatgc catattcata
     2701 gcggctgtat ttgtcatagg attcatacac gcggttgacg tccccggcga tgagcttgag
     2761 agtgacgtct tcaaaggctt tgccgctggt gttgttgatc gtcacccagg agttgagggc
     2821 cagagcttta tcgtcccaga cgctgttgta ggtgacgtcc cagctaaatc caccggtcat
     2881 ataggtgagc tggatgggga tctttcccgc tttgggagaa atcaggcccc agtgcagggt
     2941 gggcttgacg tagaagttat ccggcagctt ggccagctgg accatctggg tctcggaatc
     3001 ggagaccacg atcagacggc cggtgccgtt ttcagtgacg ccatagctgg tgccgtcaaa
     3061 gaacttcagc acgccgcgta ctctggaatt gtccttggtg agcagcacca cttccttgtc
     3121 cagatacttg gccatgatct gccatttgcc tgccaggtca tactcatagt tctgctccgc
     3181 cacggtgaag cccggttcgg tggaggtcac gatcacagac ggggcgatga ttctggaagt
     3241 gatatcgtcg tagttatagc tctggcggcc ggcttccagc tcgagctcaa aacggctgcg
     3301 caccagagaa agatcgtcgt tgtagatggt gagccaatcc tctgccacga gggaagcgct
     3361 cagcaccatc agaatcgctg agattaaaat cacgtgtttc atgttacact ccttattcgc
     3421 catgaggcga tgtcggtaat aagataatca ggattcgctg cgttcacgaa aactaaagat
     3481 cacctgggcg tcggctttca cttccacact gaaggtggct ttgttgtccg agtcgatctc
     3541 gggagtgagg tcgtgctgga taatgcgggt gttgccactc atttgatgga ccacccggat
     3601 aatcctggtc tggctggaat tgttgcgcaa agtgacctgc acggtgcgtt ccgtgacctt
     3661 cttgctcacg ttggtctggt ctttgctgag ggtgctggcc acgaggtcaa aggccttgcc
     3721 ggtggtgatt ttcacttctt cgttgcggcc ggtgtgatcg atggagtctt ccccgatgaa
     3781 ttccagattg ccgtctgtat cttctctgta gatcttgagg ctgcccttgg gaaggggttt
     3841 tccgatcccg ttggcctcgg tgtttttgaa ctggatgatg ctgttgacgg ccccgctcca
     3901 ggtgggatat tcatatatcc cttcggcttt tatgtccatg cggggataga gggccagttg
     3961 cttggtctgg ttgttggcaa agctcaactt ttggtccagg ctgtacatgt gatagtcgtg
     4021 aaagcttttc tcggcaaaag agggagcacc gccagtggct gacatgccgt caatgctgta
     4081 gttgatctca ttggcccttc cgccccggat gtggagctgt ttgaccacgt tgacgtcgcc
     4141 tgcgatgagc ttgagattgg tgtcctcaaa ggctttaccg ctggtgtttt tgattgtgac
     4201 ccaggaattg agggccaggg tgctgtcgtt ccagaccccg ttgtaggtga cgtcccagct
     4261 caggcctccg gtgaggtagc tgagctggag aggatacttt ccggcctggg gagcgatcag
     4321 gctccaacgc agagtgggcc tggtgtaaaa gttctctggc aggctggcca gctggatcat
     4381 ctgggtttcg gctgcggaga caaggtagag cctgccgtcg tagtcgtcct ggatgccata
     4441 gctgctatag tcgctgaatt tgaggatacc tctgatccgg ctgccgtctt tggtggtgat
     4501 ttccacttcg ctgttcagat atttacgcag gatttccacc ttcccggcca ggtcatattc
     4561 atagttctgc tccgctacct gaaagccctc agcctgggaa ctcacgatca cagaggcggg
     4621 catgatcctg tgagggatgt cgtcaaagtt atagtcctgg cggcctgtca tcaggttcag
     4681 ctcaaaactg gaacgcacca gggagagatc ttcattgtag atggtcagcc agttttcacc
     4741 cctcagggcc agggcgataa acatcagcag ggcagcaaga aagacacgtt tgggcataaa
     4801 agaactcctt tgccgttgag gcaggctggt aggctgaaat caaccgtgaa gccagtatac
     4861 aggagagggg atttcgtcaa ggccaaaagt ttccacatgc agccccaccg ggcaagcaaa
     4921 gcttcagcgc ttttacctga caaccgctcc gagatcctgc ccagtgattc taccaggtga
     4981 tcgggctaaa ctgatctgag ctcccgtctc caaacgcgga gcccacgctg aaacccaagc
     5041 tccgggacgc ggcaaaagaa tgtattgact tgcagccccc cgctgttttc ttgggcgcaa
     5101 gatcatcagc gaggaacgac catggaaaac atccgcatcg gcatagtggg caatattgga
     5161 gtggggaaat ccacttttat cgaagccgcc agttccgcac ccctgaacaa ggtgctcacg
     5221 tccgtctatc ccaaacccaa cggcacggaa ggggtctacg ccttcccgga aaaattcaac
     5281 cccatcgtgc tggactcctt ttataaagat cccgttgcca acgctttcat ggcccagatt
     5341 gagtttttca acggtcgcct ggaccgccag aagctgatcc agacctgccg tggcatcgtg
     5401 ctggaagaca ggaccctggc cgaggactat cacatcttcg gcaaggccca gcgcgtcctc
     5461 caaaatatgt ccgagcccga gtttatcgcc taccagcgca ctttcaggct gatgacggaa
     5521 cagatcaagg aaccggacct gctggtctat ttcaaagcgg aagttcccat cctccaggaa
     5581 aggatcagag agcgcggccg tgagagtgaa ctggccatct cagcggatta cctcgagctg
     5641 ctcaacaacc tctatgagga tttcatcgct caccacgtcc attgccccgt gttggtcatc
     5701 gacgcggacc gggcagtgga aaagacggag tggctgcgcc gcaccgtcaa cctggtggcc
     5761 gaacagatca aagccctgga gctgcgggtc tccagccccg gtatcagtga atgggtaacg
     5821 ctaccccaga cggaagccac tctcaaagcg atcgatgtgg aacgccaact ggaacagtat
     5881 ctggctgagc atcagaagct tatcactatt gccggcaacg tgggtctggg caaatccacc
     5941 ctcaccgcca ttatgggacg cagcctcaag atcaacaccc tctatgaaag accggaagaa
     6001 aaccccctcc tggaaaagtt tctgggcgat aaaaagacct ggtgttatga cctgcagatg
     6061 cattttctgc agatgcgggc ccaacaacgc cgcctgggca aaagcggaga cggcagctat
     6121 gtgaaagacc gctccctgcc ggaagacctg ctggtgtttt gcaatcagtt tcacgcggac
     6181 ggcctcctca ccgacgacga actggacaac ctcatcaccc agttccagac ggtgaaccgg
     6241 caactgccct cttcagatct gatcatcctt ttgcacggaa gcccctctct ggcctgggag
     6301 cggatccagc aacgcggccg ggaaatggaa ctcgaagggg gatggcagtt taccgagatc
     6361 aacaacctca accactggta caaagcctac gggtccaatg tggtcaaact cggtttccac
     6421 aacggtcccg tcctggaaat cgacgtcgaa aagctcgatc tcaccaaccg catccacgtc
     6481 ggctatatct tcgaacgcgt cctggagacc ctcaaagccc aggattgagg cctgtcaaca
     6541 gcctaagctc tgtcaggtgc cacatttatg atagacaacc caggcccgca atttccactc
     6601 cgagggggtt caatccccct tttgggcaca gattccaacg ccagcgcatc gactttgcgc
     6661 cccgagaaaa acctgtcctt gctgctttct tcccgcatcc tattacggac tcattacggg
     6721 tgaagtccgt aatgagtccg taatgatacc gtaatgcgaa taggagcgga agcgggcaga
     6781 attggctgtg cccgcgggcg aaaaatcaat ctaaataaca tccggacagt gatatggaaa
     6841 gattgttgag ccccgcgccg ggggcctccg tctcacaata atggtcttag cggagcggag
     6901 cttgcctccc ttccggcgat tgagcaatga gcggattgta ctggcccgac cccaaaactc
     6961 tcccttgaca tcccacccgc ctccagaatt ttggagagct atcagataaa ccgatgcggc
     7021 accagcagct tgaagcccgt gccggaggag aaaaaagtgg aatatccctt caatgagatt
     7081 gagcccaaat ggcagcaaat ctggcgtgag cgccgcgttt tttccgcccc cgatctggcg
     7141 gaccaaccca aatactatgt gctatcgatg ttcccctacc cctccggcgt gctccatatc
     7201 ggccacgctt ccaactattc catcggcgat gccgtcaccc gtctgaagat gatggaaggc
     7261 tatgccgtga tgcaacccat gggctacgat tcctttggaa tgccggcgga aaactatgcc
     7321 atcgagcata attcccatcc ccgcctcact accgaggaaa acatcgccgc catgcgcacc
     7381 cagtttgatg ccatgggctt tggcctggat tgggaacgcg aggtgagcac ctgccgtccg
     7441 gattattatc gctggggcca gtatctgttt aaaaagctct atgaaaaggg cctggtctat
     7501 cgcaagcgca gcttccagaa ctggtgcgac gcctgccaga ccgtcctggc taacgagcag
     7561 gtggaggatg gacactgctg gcgctgccat gccgacgtgc gtcagaaaga gctggagcag
     7621 tggtatttcc gcatcacgga ctatgccgaa gagctgctgg atttcagcaa gatgatcgat
     7681 tggccggagc gcgtgatcca gatgcagaaa cactggatcg gcaagagtga aggcacccgc
     7741 atcgactttg tgctggaaga ggacaacagc cccatcagcg tgtttaccac ccgtccggac
     7801 accatctacg gcgtcacttt catggccctg cctccagaac atccgctggt gcagagatgg
     7861 ctggccgcgg agccggacaa ccatgccatg cgcgcttttt gcgacaaggt gatcaacgaa
     7921 gacaagatca gcagaagcgc tgccgacacc atcaaggaag gggtgtttag cggacgctgg
     7981 tgcgtcaacc ctctgaacgg caaccgggtg cagatctgga tcaccaacta tgtgctgatg
     8041 gactatggca ccggagccgt gatggccgtg cccgctcatg atcagcgtga ctatgagttt
     8101 gccaagaaat acgacatccc cctcatcatc gtgatccaga aacctgatca cagcctggcc
     8161 atcgaaaaca tgaccgaggc ctacatcgag cccggagtga tggtcaacag cgctcccttc
     8221 gacgggctgg acagcgaagc tgctaaatcc gccatctcca ggctgatcga agaaaagggc
     8281 tggggagaag tgaccgcaac ctaccgcctc agggattggg gcatctcacg tcagcgctac
     8341 tggggcaatc ccatccccgt gatccactgt cccatctgcg gggtggtgct ggtcccggac
     8401 aaagatctgc ccgtcctgct gccggataac gtgcaggtgg gcaagacgac ctccaatccg
     8461 ctgctttccg tgccggaatg gctgcatgtg aagtgtcctc agtgcggagc agactcacag
     8521 cgcgaaacgg acaccatgga tacctttgtg gatagctcct ggtattatgc ccgctatgcc
     8581 gatcccaaaa acacagccgc tcccttcgat cccgccaagg ccaattactg gctgccggta
     8641 gaccagtaca tcggaggtat cgaacacgcc tgcatgcact tgctctatgc ccgcttcatc
     8701 ggcaagttta tgcgcgacct gggctggctg cactgcgacg agcccttctc gaggctgctc
     8761 actcagggca tggtactcaa agacggcgcc aagatgagca aatccaaagg caacgtggtg
     8821 gaccccacct acatcgtgga acgctttggg gccgataccc tgagagtcta tctgctcttt
     8881 gcctcccctc ccgataagga tgtggaatgg agcgacgacg gcatcatggg tgccttccgg
     8941 ttcctgaatc gcgtctggag gctgatcaat gccaatctgg atatcatccg caaaggcctg
     9001 caaatctggc cttctgacga ggaactggat cagctggacc ccgcgatcaa aaacctgctc
     9061 ttcagcaccc accataccac caaaaagtgg ctggacgact gcctgcagcg catgcaatat
     9121 aacaccgcca tcgcggctat tatggaacac ctcaaccact gcgtgggggt caaagaccct
     9181 caaaagcttg agggtgccgc tctggccgtc tatgccgaag cctgtggcat cgttccgcag
     9241 ctgctctatc ccttcgcgcc tcacatcgcg gaagagcttt ggcaactgat gggcatggaa
     9301 gctctgctgc acgccagcgg tctgcccggc tatgaagaac gctggctcac gcgtgacctg
     9361 gtcacctatg tggtgcaggt caacggcaag ctgcgcggta agctggaagt atcaccttcc
     9421 acagacgata acagcctcaa aactatggcc ctggagctgg aaaacgtcca gcgcgccctg
     9481 gaaggcctga gcgtgaagaa agtgatcgtg attcccggta aaatggtcag catcgctgct
     9541 gggaagtgag agagctgcgc aggttagagc agttcctgct ggttagagac ctgcagtctg
     9601 gttagaacct gcggttggtt agagacctgc ggtctggtta gagcagttcc tgctggttag
     9661 agacctgcgg tctggt
//
LOCUS       DAOSFN010000095         9596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6056_length_9596_cov_9.935751, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000095 DAOSFN010000000
VERSION     DAOSFN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6056_length_9596_cov_9.935751"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..702)
                     /locus_tag="PLO35_06875"
     CDS             complement(223..702)
                     /locus_tag="PLO35_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOH79427.1"
                     /translation="MKWYLKVGFTVLVMVTTLLIRIPVPGGGYFNFGDVVIVFAALLG
                     GSRSAALAGGIGSALADLIGFPIFAPITLVAKGLLGFLAGLGKDKPTLLSILFPVLGG
                     MAMVAVYFLGSWLMPSLGFAAALADLPANLLQSAFGITGGRLLFTAWQRLESVRTEK"
     gene            complement(699..1319)
                     /locus_tag="PLO35_06880"
     CDS             complement(699..1319)
                     /locus_tag="PLO35_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79428.1"
                     /translation="MDRLKLILAVTIFVAFCLLFGVIWLEHNCLQITALLALLALCLI
                     WLGSRKTWKQLKVIAPFAISLLLIYTLLVLLGIAPGEEKALDYWLRYGLPRLLLLVST
                     LLVFRLCVSLLSYEGLLKILPGIHWQKYLILGRILYQAAFRSYPTLKAWQELIPSAQL
                     ADKGFKPRFNRALAANLALALYVMEEARLKGEQIDNRIDCCHKEPS"
     gene            complement(1312..1803)
                     /locus_tag="PLO35_06885"
     CDS             complement(1312..1803)
                     /locus_tag="PLO35_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79429.1"
                     /translation="MIRIEAGYRQIWPSFGLDIAKELLFEQGKIYQLKGPNGSGKSSF
                     INQVLIPGLRQHDVWLLQFEQQAHLQLQAVKAWAAIFHPGLRVRDERDLVSFLLDDLE
                     RASSKRPRPIWIVADELHHLEQVWERQLSCCLIYSAHHQELTDAVPVQFEVLDPHRSV
                     VSG"
     gene            complement(1800..2147)
                     /locus_tag="PLO35_06890"
     CDS             complement(1800..2147)
                     /locus_tag="PLO35_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79430.1"
                     /translation="MDTNTGYYIADAAHPLICTGPLWEELLLCHIPSERHAELCECFE
                     RISGKAIRPSSILSELSGGQKVILMLCLAVISPAQSICFIDLEHSLDPAKLTAAMELI
                     GSSGKSIRWQTAP"
     gene            complement(2189..3466)
                     /locus_tag="PLO35_06895"
     CDS             complement(2189..3466)
                     /locus_tag="PLO35_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOH79431.1"
                     /translation="MKRYFILLAVLSLSFSCACNKKAQEQTPELASVELLPAELIKFT
                     DHNNDFAFRLYAKADEEGKNLFFSPYSISTALSMAYGGAKNNTATEMAKALSFELPEA
                     LHHSAFKDLGNRLNELGKRGKAELNVANGLFGAKLYEHLLVPEYKTLLQDFYGSDLYS
                     LDFDDAQGSAKYINDWVLDRTNQRIKDLVSPDHIKQSNHGIVLVNAIFFKGKWLLQFD
                     PKATKRESFFTKPDRAAENTKPVDLMYRQGKYGYARMPGCQVLELPYEEADLSMLMVL
                     PDEQSADKTEMTPEAYKQWRGAIKPQEVKAIIPRFTLELTLENVPRMMKSMGMIDAFS
                     DVAADFTGIRKPGSADLYIMDIIHKAFVEVKEEGTEAAAATAVVMATKSVPVFEDEIP
                     VFRADHPFYYMIIHKPSGAVLFLGKFAVPPELD"
     gene            3581..4531
                     /locus_tag="PLO35_06900"
     CDS             3581..4531
                     /locus_tag="PLO35_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH79432.1"
                     /translation="MKTTSSSEGKHLIFPIFLPMRGCPGSCIYCDQYRISGAEADMGP
                     DFDAAREFLRRHRGKARQIAFYGGSFTGLEEEQRELLLQPLLSELDEQSSFRISTHPL
                     CINPNILHWCSANRITTIELGIQDFSSSVLSASGRGYDSGTAIKAARLVNEHGFELGV
                     QLMPGLPGWDESSLAFNHLMVKDIRPQLLRIYPCLVLKDTPLHELWRQGKYQPLSLTQ
                     AISQSADWQQLCDQEGIKLIKVGLPSNLDANEIAAGPWHPAFGELVRAELLVRSLTAI
                     YPAGSIIKLDKKQRNLIMAHGGIYYNILLERIKNCSVGLS"
     gene            4584..6134
                     /locus_tag="PLO35_06905"
     CDS             4584..6134
                     /locus_tag="PLO35_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79433.1"
                     /translation="MKRLSLLLAVMALLISACEIRKPILPEWDVTLSIPLMHKNFFVS
                     DLVDSLNIIVGDDDVLTLHGTGTAETPSMGLVELTPGIQQDGIPLFSGEPRIIPVPFG
                     DTNGLVELVYGRFNSGLIRTRFNNIASQVNQISISIAELRDPAGQPFQISYQGNSGWE
                     DHSLVDSHIGELNSDEILDTLHITMTITSSLPNNSPVGTMDLNMNSPLSFGLFRGALA
                     PYDLPLMNNATTINIDYPYDLEEAIRLEHASMKIDLENQLGFACIFHGDFVAENTRTG
                     QTETIAILDDSGQPFMASAADDNGPGITELIFSEGVDSLLQIMPDRVSINNAYFTIQS
                     GIGGTVGTVRDTDKIIGNYQVDAPFTFELFASTIRLQDPVRINISEDNRIRIRDNALG
                     AELSLKIKNQLPIGVTADLYFGDTDSIDPDSLTSYQLHRVVPLRSRLWVEAHPDDPSV
                     NSDGEQLINIELSEGEVDLFANPNVFLLWTFSFEPSNGVISITASPADYISVKSMIRV
                     GLHIAEDI"
     gene            6131..7378
                     /locus_tag="PLO35_06910"
     CDS             6131..7378
                     /locus_tag="PLO35_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79434.1"
                     /translation="MKKLLILLWGSIILYPLIAGSNTGLPLMYADSYMMRAYGSEANY
                     WNPALLSEETGDIWLPALNSGIFLANNALDLDFYNYIMEKGSISEADKQKLLDMMDGS
                     AILSMSGQSSVLGFTLGNVALTSSVRYYAKAAISERFVELLLYGNEEDRTYEFTREHN
                     HVSSLSYGDITVGMGDIRLPLPQTIPDIRLGWSASLLVGIAELHTARYYGSFSSTIDG
                     FSFHQDATLRKGGGGIGFKGMLGLVSEPIENLHVGVTLDNLLGEIRWGLVRENQHYRV
                     AIDSVYVADLNEDLINYDTWTDKANAFSTKLPMEFRLGMLYKTRQISLSADYVQGFDE
                     STVTSKVGRVSVGAELLPIPILPIHLGYSPGSSTFPWRVSYGVGLRILPFEFGLGLQT
                     FESVLPSYSTKGLALASYFRFRM"
     gene            7402..9387
                     /locus_tag="PLO35_06915"
     CDS             7402..9387
                     /locus_tag="PLO35_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79435.1"
                     /translation="MPANIVIILIAALSAAFLYHRTEPPLPRWRAVLLASLRTVVLAI
                     ALMLLINPILRFIRSKTESQQIIMLHDSSLSMNNDGKLQWMKRQAKTLEGKYRQAGYE
                     VLNYSFANGLDGDPEDTQLAPALRDLAAKQDLSKASALLLFSDGWLRDENLGPVKQLG
                     LPIMPVADSSQVIQPDLAVVSLRNNRHSYRGEPTMFVAELQSGDYSGPVTVRLMTGDR
                     VLASKNLSLKASELTRVEFTHSFPSVGFYPLRVEVSAPELREKVLGNNYFPGAVDILA
                     DKERILMISDKAGWDNKFITDVITANSRWQSRSYTLQGGVIKLGEKVLSDFDRDPKAA
                     IVVINNGELKADPRLVDLIKKAHRSGTGILWQGMPLKELTDVLALGPSNVAASFQGFF
                     KLLPSSSAYPLLEIDSGELANIPPLDYYFLSPLAGAQVLAAIDTAQEPPAIAVKSIPG
                     RVINLAFLNLWRWQLQSPGSTYRQTITNLLTWLSNRGGSTYQAIHANSYFQSEQINIR
                     LRADDDIRQSRLDLNPRLKIMDADGKEVWSDFMARAEEDYVASLSLDKVGEYSFEISE
                     NGEAGKVKGRFHVAAGSLEERDHHYNLPLLSWLASESGGHLLNQTEISSLSPPLPQTR
                     KLTITNELPLYRKWYVLGLFILAFCVELFLRRRWGLL"
BASE COUNT         2170 a         2820 c         2548 g         2058 t
ORIGIN      
        1 ttacgggtgc tctacgggtc cgacccgtag agcacccgta gagcacccgt aataggaagc
       61 gggaagaaaa tctcagggac caggctacag acagagtaca atcagagcag cagtgccagc
      121 ttgtgacatc tattatggca gcgaaggcga gttctcagcc caattgctca attgacctgg
      181 agcgaagctg ccgccaacgc aaaccaggaa ctgcgtgttc ccctattttt cggtcctgac
      241 gctttccagc cgttgccagg ccgtgaagag cagcctgccg ccagtgattc caaaagccga
      301 ttgcagcagg tttgccggca aatctgccag cgcggccgcg aaacccaaag acggcatcag
      361 ccagcttccc aggaagtaaa ctgccaccat tgccattccg cccagcacgg gaaagagaat
      421 gctgagcagg gtgggtttgt ctttgcccaa tccggccaga aaacccagca aacctttggc
      481 aaccaaagtg atgggcgcga agatcggaaa gccgatcaga tctgccagag cggaacctat
      541 ccccccggcc agagcagcgc tgcgcgagcc tcccaacaga gcggcaaaca cgatcaccac
      601 gtctccaaag ttgaaatatc cccctcccgg aacgggaatc ctgatgagca gagtggttac
      661 catcaccagc acggtgaagc ccactttcag ataccatttc atgatggctc cttatgacag
      721 caatctatgc ggttatcgat ttgttcgcct ttcagccgcg cctcttccat cacatagagg
      781 gccagggcca gattggcggc cagagcacgg ttgaacctgg gtttaaagcc cttgtcggcc
      841 agctgagcgc tgggaataag ttcctgccag gctttgaggg tgggatagga cctgaaggca
      901 gcctgataca ggatccggcc cagaatcaga tacttctgcc agtggatgcc tggtaaaatc
      961 ttcagcagtc cctcatagct gagcagagag acgcaaagcc ggaaaaccag tagagtggag
     1021 accaataaga gcaagcgcgg caaaccgtaa cgcagccagt agtccagggc cttctcctcg
     1081 cccggagcga tgcccaatag caccagcagc gtgtagataa gcagcaggct gatggcaaag
     1141 ggcgcgatca ctttgagctg tttccaggtt tttctgctcc ccagccagat cagacagagg
     1201 gccagcaggg ccaacagcgc tgtgatctgc aggcagttgt gctccagcca gataactcca
     1261 aacagaaggc aaaaagccac aaagattgtg acagccagga tcagcttcag tctatccact
     1321 caccacactc cggtgggggt ccaggacttc aaactgaacc gggaccgcgt ctgttagctc
     1381 ctgatgatga gcgctgtaga tcaggcagca ggatagctga cgctcccaca cctgctccag
     1441 gtggtgcagc tcatcggcca cgatccaaat gggcctggga cgcttgctgc tagcccgttc
     1501 gagatcatcc aggagaaagc tcaccaggtc tctttcgtcg cggacccgca atccgggatg
     1561 aaagatcgcc gcccaggctt tgacggcctg caactgcaga tgggcctgct gttcaaactg
     1621 cagcagccag acgtcatgct gccggagccc cggtatgagg acctgattga tgaaagagct
     1681 tttaccgctg ccgttggggc ccttgagctg atagattttg ccttgttcaa acaagagctc
     1741 cttggcaata tccaggccaa aggaaggcca gatctgtctg tagccagctt caatcctgat
     1801 catggggccg tttgccagcg gatagatttt ccgctgcttc cgatcagttc catcgccgcg
     1861 gtcaatttgg ccgggtcgag ggagtgttcc agatcgatga aacagatgct ctgagcggga
     1921 gaaatgacgg ccagacaaag catcaggatc actttctgcc ctccggaaag ctcgctgagg
     1981 atgctgctgg gccggatggc tttgccgctg atcctttcaa aacactcaca cagctccgcg
     2041 tgccgctctg aggggatgtg acagagcaac agctcttccc agagcgggcc ggtgcagatc
     2101 agcggatggg ccgcgtccgc aatataatag ccggtatttg tgtccatgag aggacacaaa
     2161 taccggctgg cttgctgtgt caagcgcctt agtcgagctc gggcgggaca gcgaatttac
     2221 ccagaaagag caccgctccg ctgggtttgt ggatgatcat gtaataaaaa ggatgatcgg
     2281 cccggaagac ggggatttca tcttcaaaga cggggacgga cttggtggcc atcaccacag
     2341 ccgttgcggc tgccgcttcg gtgccttctt ccttcacttc cacaaaggct ttgtggatga
     2401 tgtccatgat atagagatcg gctgatccgg gtttgcgaat gccggtaaaa tcagccgcca
     2461 catcactgaa ggcatcgatc atgcccatgc tcttcatcat ccggggcacg ttttccagcg
     2521 tgagttccag ggtgaagcgt ggtatgatcg ccttcacttc ctgcggtttg atagccccgc
     2581 gccactgttt ataggcctca ggggtcatct ctgtcttatc ggcgctttgc tcatccggca
     2641 gcaccatcag catgctgagg tctgcttctt catagggaag ctcgagcacc tggcagccgg
     2701 gcatcctggc gtagccgtat ttgccctggc gatacatcaa atccaccggt ttggtgtttt
     2761 cagccgcccg gtccggcttt gtaaagaagc tttcccgttt ggtggccttg gggtcaaact
     2821 gcagcagcca cttgcccttg aagaagatag cgttcaccag cacgatcccg tgattgctct
     2881 gtttgatgtg atcgggcgag accagatctt tgatcctctg attggtgcgg tccagcaccc
     2941 agtcgttgat atacttggca gagccttgcg cgtcgtcaaa atccagagaa tagaggtcgc
     3001 tgccataaaa atcctgcagc agggtcttgt attccgggac cagcagatgc tcatagagct
     3061 tggcgccaaa cagcccattg gccacgttga gctccgcttt gcctcttttg cccagttcgt
     3121 tgagccggtt gcccagatct ttaaaagcgc tgtgatgcag ggcttcgggc agttcaaagg
     3181 agagggcttt ggccatttcg gtggcggtgt tgtttttggc cccgccgtag gccatcgaaa
     3241 gggcagtgga aatgctgtaa ggagagaaaa agaggttctt cccctcctca tcggccttgg
     3301 catagagccg gaaggcaaag tcgttgttgt ggtctgtaaa cttgatcagc tcggcaggca
     3361 acagttcaac cgatgccagt tcaggagtct gctcctgggc ttttttgttg caggcgcagc
     3421 taaagctgag gctcagcacc gccagcagga tgaagtaacg tttcatcata aagatcctcc
     3481 ctggatgatc tggagcgcag tttatcttct ggcacaaagg cgtcaacata tttttggaga
     3541 ggacttgacc gcgcagggca tccggcttag attatcccct atgaaaacca cgtctagctc
     3601 cgagggcaag cacctcatct ttccgatctt tctgcccatg cggggctgcc cgggaagctg
     3661 tatctattgc gatcagtatc gcatcagcgg cgcggaagcg gacatggggc cagactttga
     3721 cgcggcccgg gaatttctca gacggcatcg gggcaaagct cgccagatag ctttctacgg
     3781 aggcagcttc accggtctgg aggaagaaca gcgggagctt ctgctccagc ccttgttgtc
     3841 tgaactggat gagcagagct cgttcaggat ctccacccat cccctctgta tcaacccaaa
     3901 tatcctccac tggtgctcag ccaatcggat caccaccatc gagctgggaa tccaggattt
     3961 ttccagctcc gtgctctctg cttccggacg cggttacgat tctggcacag ccatcaaagc
     4021 agcccggctg gtgaacgagc acggcttcga gcttggggtc cagctgatgc ctggcctgcc
     4081 gggctgggac gaaagcagcc tggccttcaa ccacctcatg gtcaaagata tacggcccca
     4141 gctgctgagg atctatccct gcctggtgct caaagacact cctctgcacg agctctggcg
     4201 ccagggcaag tatcaaccgc tcagcctcac ccaggccatc tcacagagcg cggactggca
     4261 acagctttgt gatcaggaag gcatcaaact gatcaaagtg gggctgccgt caaacctcga
     4321 tgccaatgag attgctgccg ggccctggca tcccgctttt ggggaactgg tcagggccga
     4381 gctgctggtc cgctccctca cggcgattta tccggccggt tcaataatta agctggacaa
     4441 aaaacaaagg aacctgatca tggctcacgg tggtatttat tataatatcc tgctggaacg
     4501 gatcaagaat tgctccgttg ggctatcgtg atttattcag catcaaggcg atttatatgc
     4561 atccgcataa aaggaacagc attatgaaaa gactgagctt gctattggca gtaatggcgc
     4621 tgttgatcag cgcttgcgag attcgcaaac cgattctgcc ggagtgggat gtgacgctca
     4681 gcatccctct catgcataaa aacttctttg tttcagatct ggtggacagc ctcaatatca
     4741 tcgtggggga tgacgatgtg ctcaccctcc atggtaccgg cacggctgaa acaccgtcca
     4801 tgggcttggt tgaactcact cccgggatcc agcaggatgg aatccctctg ttctctggag
     4861 agccacggat cattccggtc ccctttggag ataccaatgg cctcgtggag ctggtctatg
     4921 gacgtttcaa cagtggtttg atcaggaccc gcttcaacaa cattgcctcg caggtaaacc
     4981 agatcagcat cagcattgcc gaactccgcg atcccgccgg acagcctttc cagatatcct
     5041 accagggaaa ctccggctgg gaggatcaca gcctggtgga ttcccacatc ggagaactca
     5101 attcggacga gattctcgat acactccaca tcaccatgac catcacgtcc tcgctgccca
     5161 acaactctcc ggtgggcacc atggacctca acatgaacag ccccctcagt tttggcctgt
     5221 tcagaggagc gctggcaccc tacgacctgc ccctgatgaa caacgccacc accatcaata
     5281 tcgactaccc ctacgacctg gaggaagcca tccggcttga gcatgccagc atgaagatcg
     5341 atctcgagaa tcaactgggc tttgcctgca ttttccacgg tgattttgtg gcggaaaata
     5401 cccgcaccgg ccaaactgag actatcgcca tcctcgacga cagcggccag ccctttatgg
     5461 ccagcgctgc ggatgacaac ggccccggga ttaccgaact gatcttttcc gagggggtgg
     5521 attcgctctt gcagatcatg ccggaccggg taagcatcaa taacgcctat ttcaccatcc
     5581 agagcggtat cggtggcacg gtcgggaccg tgagagatac agacaagatc atcggcaact
     5641 atcaggtgga cgcgcctttc acctttgagc tctttgcctc cacgatccgt ctgcaggacc
     5701 ccgtgaggat aaacatctcg gaagacaacc gtattcgcat ccgggacaac gccctgggcg
     5761 cggagctctc gctcaagatc aagaaccagc tgcccattgg cgtcactgcc gatctgtatt
     5821 ttggagacac ggactccatc gatccggaca gcctcacatc ctatcagctt caccgcgtcg
     5881 tcccgctcag gtccaggctg tgggtggaag ctcatccaga cgatcccagc gtcaacagcg
     5941 atggtgaaca gctcatcaac atcgagctct cggaaggcga agtggacctt tttgccaatc
     6001 ccaacgtctt cctgctctgg accttcagtt tcgagcccag caacggcgtg atctccatca
     6061 ctgcctcccc ggctgattac atctcggtga aaagcatgat ccgggtgggc ctgcacatcg
     6121 cggaggatat atgaaaaagc tcctcatcct cctctgggga tcgatcatcc tgtatcccct
     6181 gatagctggc agcaatactg gcctgccttt gatgtacgcg gacagctata tgatgcgggc
     6241 ctatggcagc gaggccaact actggaatcc cgccctcctc agtgaagaaa caggagacat
     6301 ctggctgccc gctctcaatt cagggatctt tctggccaac aacgcgttgg acctggattt
     6361 ctacaactac atcatggaaa aagggagcat ctccgaagcc gacaagcaga agctgctgga
     6421 tatgatggac ggcagcgcta tcctcagtat gagcggccag agcagcgtcc tgggcttcac
     6481 ccttggcaac gtggccctta catcaagtgt ccgctattat gccaaagccg ccatctccga
     6541 acgctttgtg gaactgctgc tctatggcaa tgaggaagac aggacctatg agtttacccg
     6601 ggaacacaat catgtgagct cgctcagcta tggagacatc acggtgggca tgggagatat
     6661 ccgtctgcct ttgcctcaga cgattccaga tatcaggctg ggctggtctg ccagtctcct
     6721 ggtcgggatc gcggaactgc acacggcccg ctactacggc tccttcagct ccaccatcga
     6781 cggcttttcc ttccatcagg acgcgaccct ccgcaaaggt ggaggaggaa tcggcttcaa
     6841 aggcatgctg ggactggtct cagagcctat agaaaacctc catgtgggcg tgacgctgga
     6901 caacctcctg ggtgagatcc gctggggact ggttcgcgaa aatcaacact accgcgtagc
     6961 tatagacagc gtctatgtgg ccgacctcaa tgaagacctc atcaactatg atacctggac
     7021 cgacaaggcc aacgccttca gcaccaagct tcccatggaa ttccgcctcg ggatgctgta
     7081 caagaccagg cagatcagcc tttccgccga ttatgtgcaa ggttttgacg aatccaccgt
     7141 cacctccaaa gtaggcagag ttagcgtggg agcggaactg ctgcccatcc cgatcctgcc
     7201 tatccatctt ggttattcac ccggcagcag cacgttcccc tggcgggtat cttatggcgt
     7261 agggttgcgc atcctgccct tcgaattcgg gctgggattg cagacctttg aatcggtgtt
     7321 gcccagctac agcaccaaag gactcgccct ggccagctat tttcgcttta ggatgtagcc
     7381 ctggctctag ccgtttcagt aatgccagct aacatcgtca ttatcctcat cgctgccctc
     7441 agcgcggcgt ttttgtatca tcgcaccgag ccgcccctgc ctcgctggag ggcggtgctt
     7501 ttggcctctt tgcgtacagt ggtgctggcc attgccctga tgctgctgat caatcccatc
     7561 ctgcgattca tccgctccaa aaccgagagc cagcaaatca tcatgctcca cgatagctcc
     7621 ctcagcatga acaacgacgg caagcttcag tggatgaaac gccaggccaa aacgcttgag
     7681 ggcaaatacc gccaggctgg ctatgaggtg ttgaactaca gctttgccaa cggactggac
     7741 ggcgatccgg aagataccca gctggcccct gccctcagag acctggccgc caaacaagac
     7801 ctctccaaag cctccgctct gctgctcttt tccgatggct ggcttcggga tgaaaacctc
     7861 ggtcccgtca aacaattggg cctgccaatc atgccagttg cagacagcag ccaggtcatc
     7921 caacccgacc tcgcggtcgt cagtctgcgc aacaaccgcc acagctatcg tggcgagccc
     7981 accatgtttg tggcggaact gcagtccgga gactacagtg gtcccgtcac ggtccgtctg
     8041 atgacgggag acagggtgct ggccagcaaa aacctcagcc tcaaagcctc agaactcacc
     8101 cgggtggagt ttacccacag ctttccctcc gtgggtttct atccgctcag ggtggaggtc
     8161 agcgctccgg aactcaggga aaaggtgctg ggcaacaatt acttccccgg ggccgtcgat
     8221 atcctggctg ataaggaacg gatcctgatg atctccgata aagccggttg ggacaacaag
     8281 ttcatcaccg atgtgatcac tgccaacagc cgctggcagt ctcgaagcta caccctccag
     8341 ggtggagtga tcaaactggg tgagaaggtg ctcagcgatt ttgaccggga tcccaaagct
     8401 gctatcgtgg tgatcaacaa cggcgagctc aaggccgatc cgcgtctggt ggacctgatc
     8461 aaaaaagccc atcgctccgg gactggcatc ctctggcagg gtatgccttt gaaagaactc
     8521 acggacgtcc tcgctctggg tccttccaac gtggcagcca gctttcaggg cttcttcaaa
     8581 ctcctgccct cctcttccgc gtatccgctt ctggagatcg actccggaga gctggccaac
     8641 atcccgcccc tcgattacta ttttctcagc ccgctggccg gagctcaggt cctggccgcg
     8701 atcgatacgg cccaggaacc tcccgccatt gcggtcaaat caatccccgg cagagtgatc
     8761 aacctggcct ttctcaacct ctggcgctgg caactccaga gccctggcag cacctatcga
     8821 cagaccatta ccaatctcct cacctggctt tccaaccggg gtggttccac ctaccaggcc
     8881 atccatgcca acagctattt ccagagtgag cagatcaaca tccgcctccg ggccgatgac
     8941 gatatccgcc agagccggct ggaccttaat ccccgcctca aaatcatgga cgcggacggc
     9001 aaagaggtct ggtccgactt tatggcccgc gccgaagaag actatgtcgc ttcgctaagc
     9061 ctcgataagg taggagaata cagctttgag atcagcgaaa acggcgaggc cggcaaggtg
     9121 aagggacgtt tccatgtagc tgccggttcc ctggaagaac gcgatcacca ctacaacctg
     9181 cccctgttga gctggctggc cagcgagagc ggaggccacc tcctcaacca aacagagatt
     9241 tcttcccttt ccccgcctct tccccagacg cgtaagctaa ccatcaccaa tgaattgccc
     9301 ctctatcgca agtggtatgt gctgggcctc tttatcctgg ctttctgcgt ggaactcttt
     9361 ctgcgccggc gctggggact gctgtaaact ctatcatcag caagagtctg gttgcattat
     9421 tgtaactaca agtttctccc tcaaggcccg atcaggaaag ccccttccat aaggcagcag
     9481 atccatcagt gtaaagctga gacacactct cccgaaacac gaactgctga cccgggatac
     9541 ccaacttgcc tgatatgtct ctagaggacg taatggagga taaccaatac cccccc
//
LOCUS       DAOSFN010000096         9580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6071_length_9580_cov_9.285774, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000096 DAOSFN010000000
VERSION     DAOSFN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6071_length_9580_cov_9.285774"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..426)
                     /locus_tag="PLO35_06920"
     CDS             complement(109..426)
                     /locus_tag="PLO35_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOH79436.1"
                     /translation="MIEEIISIQSGAEDIDRLIHEPARLNIMLYLYVVEEANFVFLQR
                     NTGLTRGNLSVHLQKLEQGGYVEISKKFVDRKPSTLARLTKDGRKAFEEYRSRILGLL
                     KLP"
     gene            complement(419..1018)
                     /locus_tag="PLO35_06925"
     CDS             complement(419..1018)
                     /locus_tag="PLO35_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79437.1"
                     /translation="MKDELVEKTREQVTQDIKYRDGIPRIAAGVFMVIAMLLALAGNT
                     STFVIFIPIIPGLVEGLRKRYTYPRVGYAKIKEGQSSKRLLILIALALVIGLLVFLIS
                     NGMLGFELPSKVNIYGPMALGVAMPLVLIALLLTARRRQQWPFTLITILVFLPVLLFV
                     RLGRHTVFYIVLAFGVLNLIMGIIELRRFIKDYPVIPDD"
     gene            complement(1571..6097)
                     /locus_tag="PLO35_06930"
     CDS             complement(1571..6097)
                     /locus_tag="PLO35_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOH79438.1"
                     /translation="MKPRALAILMFFVMVLTLSAAALPKVPTNRPAYASDLIKVKLSP
                     DAVQRAALPQGLYAESNSFGVNELDQLMSVNGGTKVIRAHRRLKDTAWEARTGFDRWF
                     LIKLDGRASVDQALASFRANRYVEDATYEYYAYPSAVPNDPQYSTHWGHNNTAQFPSW
                     NSSTYQFTGPAVGIAGFDVDAQLAWDLPQGYGSADIVVAIIDSGVDTSHPDLRQVTGY
                     DFGDNDTNPMDDATDAGHGTLCAGVTAAIANNNLGVVGIAGGCSIMPLKVADSDGNMA
                     FTSIANAITYAVDHDADVISMSLGANITSDSTVDPTLTYANNNGVVVLAATGNYYDPA
                     YISGNQIQDVVSYPAVNQYVISVGAASPGAERKSYTSVDNVVYWGSCYGTNVQDDRLS
                     VDIMAPSLLPSTDIQSTAGWNHSSGTAGNYYSYFGGTSCATPYAAGVAALLLSYDPTL
                     TPAEIRNALVTTATDMTIDGGVGWDRYTGYGMINAYQALLTLSPGQPTCQITAPTGGS
                     VHDLNSSIQINVSATDSDGTISNVKFYVDDVLKNTDSSTPYSWTWDTPGYSGGSHTIK
                     AVATDNDLNTRESTVTITLLAPPDEGFESGNFGAFSWNNSSAVAWTVQSSEKYSGSYA
                     AKAGPIGNSASTTLSITLNVTTAGDLSYFYKVSSESGYDYLRFYLDDVQQAQWSGEAG
                     WALQTHAISTGLHSFKWTYSKDVSYLGGSDTAWLDHIIFPEFGAYYPPPRNLAAVGGN
                     GFVNLSWQAPDGGTPTSYKIFKNGSELSTTSNLYYTDNAVINGNTYSYYLTAVYSGGE
                     SDPTATVNATPNVVSSVTIGSGTSSTATSAACPINVYYQSLHGQSVYTKAELNTAGAT
                     GPIQITQIGFNVTGLPTKVMPNFVVRMKHTTATNVASWIDNSNLVTVYSNATYQPTTT
                     GWNMLTLTNPFTWNGSDNILVDTAFGLIGSWDQSGVVQYTTVTSGYRYTRSDTVDQTN
                     VFSGSGTNNTTSTYRPNIKLTLSGSITPVPFAQGFESGLGNWLFANGSQTNKWARSTA
                     TANTGDTSLYISNNGGTSNAYTTSSASIVHAYSAVSFPSGSESWKLRFNWKAQGESST
                     DYLKVYLVDATTTPVAGTLLSSGQLGGTYNLSSAWQEVTLDIPAGVNGQTRLLVFSWA
                     NNSSGGTNPPAAIDDIRIVPGSQSDAAVVIDNSVTVDPPAVTDPESNVINTSVEITGI
                     TETEGYVLVITGYDSIGAPYDDAGLDFVITGVDLAGSTITFAHGLGFVPTTLAYKVGD
                     SGAWNIVTNPGGWTDASAWFTVPAAKAEGDIYAAFPKNGDDTLPVELSSFTATLNAYN
                     KVLLTWVTQSEDNCMGYHIFRNSSDALDSAIDLQTLVEATNTSQQQVYIFEDAELQED
                     GTWYYWLQSLDMDGSHAFFGPISIAYSNGGGNNSPGVPLVTELLSAYPNPFNPSTTIR
                     YALAEPGKVEIVIFNTRGQKVREFSIEHSQPGFFKLIWDGKDSYGNMAASGLYFYRMN
                     TARFHQLRKMVLAK"
     gene            complement(6222..8279)
                     /locus_tag="PLO35_06935"
     CDS             complement(6222..8279)
                     /locus_tag="PLO35_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79439.1"
                     /translation="MKKLALLCAVLTAVACLAALPPEYDVSIQPTLIRESWYDFMMGS
                     YNNLPMQELPSQFGGGRFFTYHARLTQTGLRKVWFGYINDAGLLAPMDDPWIATSVYM
                     GYPALSVDRELGKPFYAWHMSNDADTEYEIAFMYDMYPQQTPGIYSSLQYPFDPPNTP
                     PGHENDEFLWATLQTGPSPNPGMRRVYVMVRNYAYDDHPSENVLIAYADYNEAMLMAD
                     QSFTWSYTSIPQLDDWHQTTEPVYRRPAGSLAVGDDGRIYYAGLHYARDADSELIDEP
                     DIDVFVCDNYGAGTWQYYSASSKVPSYNPWNPFLMRHAFYDDAEEPIPDEEIYYQTAF
                     VRHFNISLDSDGKLHIPNIWSLQIGDPGLAYRLFSTIKEAVFDTATHTFEIREIYPKA
                     GNSIDDIWWMPWDGDGDHQADNWPLDQLQPEFTYNFPYCHWDETLHFDAMIFHYGNLH
                     ISEDDGSGAMVCLWEDSYKARCYNLYPADYPQYQPWGQAPEVMISVSPDRGLSWSDPI
                     VLSSVDTPELAGMIPMWVYPSDRMQSVNSGGNPAKRLWLMFMDDYDWGTTAITPTPLP
                     PNRGDIMYMALDIQMPPSAIPDEPLPGPETFGLSNYPNPFNPSTTITYELAKAGRTRL
                     AVYNLRGQMVNILSDEQQSSGTHKIVWDGQDSHGRSLSSGVYLLKLETDGKTALHRMM
                     LVK"
     gene            8433..9521
                     /locus_tag="PLO35_06940"
     CDS             8433..9521
                     /locus_tag="PLO35_06940"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HOH79440.1"
                     /translation="MRKTAVTITLIIMLSLLLADLINPAGSVIFGDLRDTDGSLSFSV
                     RTATYNGAYAPRNAGVIWITNSQNQFIKTIKIWANSYRYTLIRWIASSGQNTTGAVTS
                     ASYNNHQLHNVTWNGKNYQNQDVPDGEYKINVEFTEHNANANNLGKFKQVTFVKGPDP
                     IDITIPNESYFRDMHLVWQPVIQNGIISGYVRDESNQPIQGAIIQAGAFSTSSQSDGH
                     YSLTVPPGTYTVVCLNTGYQDIIATDVVVTSNQTTTQDFIMMTVANDDPLSPAASLSL
                     KAPYPNPFHQETTIRISLPKAVPYTIEIFNLRGQKLISRQMIPQSKGEQQLSWDGRDM
                     FGKPCPPGIYQISINQGTQKICTRVTLR"
BASE COUNT         2190 a         2439 c         2673 g         2278 t
ORIGIN      
        1 ggcaaaggag aggcataggt ggggcatagg tggtgtaaag gaggctaagg gcaaaacatt
       61 gaaaaactgt aagatgcttt tcgcggaagg cacccgcgcc actcctactc agggcagctt
      121 caacagcccc aaaatcctgg accggtactc ctcaaaggcc ttgcggccgt ctttggtgag
      181 gcgcgccagg gtgctgggct tgcggtccac gaatttcttg ctgatctcca catagccgcc
      241 ttgttccagc ttttggaggt ggacggagag gttgcctctg gtgaggccag tattacgctg
      301 cagaaatacg aagttagctt cttccaccac atagagatag agcatgatgt tgaggcgggc
      361 cggctcgtga atgaggcggt ctatgtcttc cgcgccgctc tggatgctga tgatctcttc
      421 aatcatctgg aatcaccgga tagtctttga tgaaacgccg caattcgatg atccccatga
      481 tcaggtttag taccccaaag gccagcacga tataaaaaac ggtatgacgg cctagcctca
      541 cgaagagcaa gaccggcaga aaaaccagta tcgtgataag ggtgaagggc cattgctgtc
      601 ggcgcctggc cgtcagaagc aaggcgatca gcacaagcgg catggctact ccaagagcca
      661 tggggccata gatgttaacc tttgagggta gctcaaaccc cagcatccca ttgctgatca
      721 aaaacaccag aaggccaatc accaaggcca gtgcgatcag aattagcaat ctcttgctgc
      781 tctggccttc tttgattttg gcataaccca cccggggata ggtgtagcgt ttgcgcagtc
      841 cttccaccag gccaggaatg atggggatga agatcacaaa agtgctggtg ttgccggcca
      901 gagccaacag catggcaatc accataaata ctcccgcagc tattcggggt atgccatccc
      961 ggtatttgat gtcctgggtg acctgctcgc gggttttctc gaccagttcg tctttcattg
     1021 tgcctccacg ggctatagac ttgtttatgg tgcaaacatg tttaccgtgg tctgggagtc
     1081 aagatgtttg tgtgcgattt ctgatcacac gtcgcctgag ctgtggaaat ctttccacgt
     1141 gcaggccggg tctgccacgc tccttgagac cgttgaataa tcggtatcac tggctgaaga
     1201 gcttgtgaaa aaactggcga agccggaggt tgacattacc ccgttggagt tcaagcggcc
     1261 acgataaaag catgcaagac agcgagtttg cgggccgctt ggacttcaac cagtacaccc
     1321 gcgagataaa tgtcttcgat gtgctgcacg taagtccaaa gccccgtcag gtatagagat
     1381 agacgcataa tctatttagg ggctttgaac tccaagacgg tcaaggtagt ttttttttca
     1441 caagctctga agccagtgtt acggttatgt gcaacggtct ttccttgagg tttcctccag
     1501 atcaaaacag cataatgaaa aagcggagac cttccggtct ccgctgtaac gtctctagga
     1561 tgaaggatat ctacttggcc agcaccatct ttctcaactg atggaacctg gctgtgttca
     1621 tgcggtagaa atacaaaccg ctggccgcca tattgccata gctgtctttg ccgtcccaga
     1681 tcagtttgaa aaatccgggt tggctgtgct cgatgctgaa ttcgcgtacc ttctggcccc
     1741 gggtgttgaa gatcacgatc tccaccttcc caggctctgc gagggcgtag cggatcgtgg
     1801 tgctggggtt gaagggattg ggataagcgc tcagcagttc cgtgaccaga ggcactccgg
     1861 gactgttgtt tcctcccccg ttgctgtagg caatgctgat gggaccaaag aaggcatggc
     1921 tgccgtccat atcgaggctt tgcagccagt aataccaggt gccatcttcc tgcaactcag
     1981 cgtcttcaaa gatatagacc tgctgttggg aagtgttggt ggcctccacc agggtctgca
     2041 gatcgatggc gctgtccaga gcgtcgctgg aattgcggaa gatgtgataa cccatgcagt
     2101 tatcctcgga ttgggtcacc caggtgagca gcaccttgtt gtaagcgttg agggtggcag
     2161 tgaaggatga cagctcaacc ggcagggtgt cgtctccgtt tttggggaag gcggcataga
     2221 tgtctccctc cgctttggcg gctgggactg tgaaccaagc ggaagcatca gtccatccac
     2281 ctgggttggt cacgatgttc caggcaccgc tgtctcccac tttataggcc agcgtggtgg
     2341 gaacgaagcc caggccgtgg gcgaaagtga tggtgctgcc agccagatcg actccggtga
     2401 tcacaaaatc cagtccggca tcgtcatagg gagcgccgat gctgtcgtag ccggtgatta
     2461 cgaggacgta gccctcggtt tctgtgatgc cggtgatctc gacagacgtg ttgatcacat
     2521 tgctttccgg gtctgtaacg gcaggcggat ccactgtaac gctgttgtcg atcaccaccg
     2581 ccgcgtcgct ttgtgagcca ggcacgatgc ggatatcgtc tatggcggct ggggggttgg
     2641 ttcctccgct ggagttgttg gcccagctga agaccagcaa cctggtctgg ccatttaccc
     2701 cggcgggaat atccagagtc acttcctgcc atgcggatga aaggttgtag gttccaccca
     2761 attgtccact gctcagcagg gtccctgcca ctggagtggt ggttgcgtcc accagatata
     2821 ccttgagata gtctgtgctg ctttcacctt gagctttcca gttgaagcgc agcttccagc
     2881 tttcggaacc ggaggggaag ctgacagcgc tataggcgtg gacaattgag gcgctgctgg
     2941 tggtgtaggc gttgctggtg cctccgttat tggagatata gaggctggta tcacctgtat
     3001 tagcagtggc agtgcttctt gcccatttat tggtctggct gccgttggcg aacagccaat
     3061 tgcccaggcc gctttcgaag ccctgagcga aggggactgg agtgattgat ccggataagg
     3121 ttagtttgat attgggtcgg taagtggaag tagtgttgtt tgttcctgat ccggagaaca
     3181 cattagtttg gtctacagta tcactgcgag tgtatctgta accgctggta acggtagtgt
     3241 actgtactac tccagattgg tcccaggaac cgatcagccc gaacgctgta tccaccagga
     3301 tgttgtccga gccgttccag gtgaagggat tggtgagggt cagcatgttc cagccggtgg
     3361 tggtgggctg gtaggtagca ttggaataga ctgtcaccag gttgctgttg tctatccagg
     3421 aagccacatt ggtggcagta gtgtgcttca tccgcaccac gaagtttggc atgaccttgg
     3481 tgggcaggcc tgtaacgttg aatcctattt gggtgatctg gatggggcct gtagcgccag
     3541 cggtattaag ctccgctttc gtgtaaactg attgaccatg cagactttga taatacacgt
     3601 tgatcgggca ggccgcgctg gttgccgtgc tggaagtgcc gctaccgata gtaacggaag
     3661 atactacatt aggagtggcg ttcaccgtgg cggtgggatc gctttcccct ccggaataga
     3721 ccgctgtgag gtaatagctg taggtattac cgttgatcac ggcgttgtcc gtgtaataca
     3781 ggtttgaggt ggtggaaagc tcgctgccgt ttttgaatat cttgtaggaa gtgggggtgc
     3841 ctccgtccgg agcttgccag ctaaggttca caaagccatt tcctcccacc gctgccaggt
     3901 ttctgggagg cgggtaatag gcaccgaatt ccgggaaaat gatgtggtcc agccaggctg
     3961 tgtcgcttcc cccaagatat gatacgtcct tggaataggt ccacttaaag ctgtgcaaac
     4021 cggtgctgat ggcatgggtt tgcaaggccc atccagcttc accggaccac tgagcctgct
     4081 gtacgtcgtc taaataaaag cgtagatagt catagccaga ttctgaggat accttgtaga
     4141 aatagctaag gtctccggca gtggtcacat tcagggtgat agacagtgta gtgctggcgc
     4201 tgtttcctat tggacccgct ttggcggcat agctgccact gtatttttca ctgctttgca
     4261 ccgtccaggc tacggcactg ctgttgttcc agctaaacgc gccaaagttg ccactctcaa
     4321 aaccttcatc gggaggggcc aacagagtga tggtaacggt ggattcccgg gtgtttaaat
     4381 cattatcggt ggccacggct ttgatggtat ggctaccacc agaatagcct ggggtatccc
     4441 aggtccagct atagggagtg ctggaatctg tgttcttcaa cacatcgtcc acatagaatt
     4501 taacgttgga tatcgtaccg tcgctgtcgg tagcgctcac gtttatctga atggagctgt
     4561 tgaggtcatg cactgatcca ccggttggag cagtgatctg gcaggtgggt tgcccggggg
     4621 ataaggtcaa cagagcttga taggcattga tcatgccata tcctgtatag cggtcccagc
     4681 caaccccgcc atcgattgtc atgtccgtgg cggttgtgac caaagcgttg cggatttccg
     4741 caggagtgag ggtggggtca taggaaagca gtaaagctgc caccccagcg gcataaggtg
     4801 tggcacagga tgtgccgcca aaataggaat aatagttacc cgcggtaccg ctgctgtgat
     4861 tccatccggc tgtgctctga atatctgtgg aaggcaacag actgggagcc atgatatcaa
     4921 cggacagtct gtcatcctgt acatttgtgc cgtagcatga tccccaatac accacattgt
     4981 ccaccgatgt atagctcttt ctttctgccc ccgggctggc agcaccgaca ctgatcacat
     5041 actggtttac tgctggataa gataccacgt cctggatctg gtttccggat atataggcag
     5101 ggtcatagta gtttccagtt gctgccagga caactactcc gttgttattg gcgtaggtaa
     5161 gtgtaggatc aactgtggaa tctgacgtta tattggcacc caggctcatg cttatcacgt
     5221 cagcatcgtg atctacagca taagttatcg cgtttgctat ggatgtgaat gccatattgc
     5281 cgtctgagtc agcaactttg agtggcatga tactgcaacc cccggctata ccaacaaccc
     5341 ccagattgtt attggcaatg gcagctgtta ctccagcgca tagagtgcca tgaccagcat
     5401 ctgtggcatc atccatcggg ttcgtatcat tatcaccaaa gtcataacct gtcacctgcc
     5461 ttagatcggg gtgggaagta tccacaccgg aatctatgat tgccactacg atatcggcag
     5521 atccataacc ctgaggaaga tcccaggcta gttgagcgtc cacatcgaat ccagctatgc
     5581 ctacggcagg ccctgtgaat tgataggtgc tggaattcca ggatgggaat tgggctgtat
     5641 tgttatggcc ccaatgggtg gaatactgag gatcgttggg caccgcggaa ggataggcat
     5701 aatactcata agtagcatct tccacatagc ggttggcgcg gaaagaggca agcgcctgat
     5761 ccaccgatgc ccgcccatcc agtttgatca aaaaccagcg gtcaaagcca gtacgggctt
     5821 cccaggctgt atctttgagc cgacggtggg ctctgatcac tttggtgccg ccgttgacac
     5881 tcataagctg atccagttca ttgaccccga aggagtttga ctccgcatag aggccttgcg
     5941 gtaaagcagc gcgctggacg gcatcaggag aaagcttcac cttgatcagg tctgaagcgt
     6001 aagccggacg gttagtgggg accttgggta acgcggccgc cgacaaagtc aggaccatga
     6061 caaaaaacat cagaattgct agggctctcg gtttcattaa accatctctc ttacaggttg
     6121 ttacattata gcaactatgc tagagagctg atttattctg tcaacgagtt tttttagcag
     6181 gttttgggga tgtgtggcct tttttcctca atacatgcca atcacttcac cagcatcatt
     6241 ctgtggaggg ctgtcttgcc atcggtttcc agcttcagga gatagactcc cgagctcaat
     6301 gaccggccat gggaatcctg cccatcccag acgatcttat gcgttccgga gctttgctgc
     6361 tcatcgctga ggatattcac catctggcct ctgaggttat aaaccgccag cctggtcctt
     6421 ccggctttgg caagctcgta agtgatcgtg gtggaagggt taaaggggtt gggatagttg
     6481 ctcagtccaa acgtctccgg tcccggcaaa ggttcatcag gtatcgcgct gggaggcatc
     6541 tggatatcca gggccatata cattatatct ccccgattgg gaggaagggg agtgggtgtg
     6601 atggcagtcg ttccccagtc atagtcgtcc ataaacatca gccacagccg tttagctgga
     6661 ttcccaccgg agttcacaga ctgcatgcgg tcggagggat agacccacat agggatcatg
     6721 ccagccagct cgggagtgtc aactgatgaa aggacaatgg gatcgctcca ggataggccg
     6781 cggtccgggc tcacggaaat catcacttca ggggcctggc cccagggttg gtattgcgga
     6841 taatcggcag ggtagaggtt gtagcagcgc gccttatagc tgtcttccca gaggcagacc
     6901 atggccccgc tgccgtcgtc ttcgctgata tgcagattgc cgtagtggaa gatcatagcg
     6961 tcaaagtgca gcgtctcgtc ccagtggcaa taggggaagt tgtaagtaaa ctcaggttgg
     7021 agctggtcca gcggccagtt gtcggcctga tggtctccat ccccgtccca gggcatccac
     7081 cagatgtcat cgatgctgtt tccggctttg gggtagatct ccctgatctc aaaagtgtgg
     7141 gtggcggtat cgaacacggc ttccttgatg gtggaaaaga gccggtatgc aaggccggga
     7201 tccccaattt gcagagacca gatattgggg atatggagtt tgccgtcgga atccaggctg
     7261 atgttgaagt gcctcacaaa agcggtctgg tagtagatct cttcatccgg gatgggctct
     7321 tccgcgtcgt catagaaggc gtgacgcatc aggaagggat tccagggatt gtaggacggc
     7381 accttgctgg aagcgctgta gtattgccag gtgccggctc catagttatc gcacacaaag
     7441 acatctatat ccggttcgtc gatcagctca gagtctgcat cccgggcgta atgcaagccc
     7501 gcgtaataga ttctgccgtc gtccccaaca gccagactgc cagcgggcct gcggtagaca
     7561 ggttccgtgg tttgatgcca gtcgtccaat tgcggaatgc tggtatagct ccatgtgaaa
     7621 ctctggtccg ccatcagcat cgcttcgttg tagtcggcat aggcgatcag gacgttttcg
     7681 ctgggatgat cgtcgtaagc gtagtttctg accatcacat aaacccttcg catcccgggg
     7741 tttggagaag gaccggtctg cagcgtagcc cagaggaact catcgttttc atgcccggga
     7801 ggggtattgg gagggtcgaa aggatactgg aggctggaat agatgccggg cgtctgttgt
     7861 gggtacatat catacatgaa ggcgatctca tactccgtgt ccgcgtcgtt gctcatgtgc
     7921 caggcataga aaggcttgcc cagctcgcgg tccacgctca gggccggata gcccatataa
     7981 actgatgtgg ctatccaggg atcgtccatg ggggccagca atccggcatc gttgatgtag
     8041 ccaaaccaga ctttgcgcag gccggtctga gtgaggcgcg cgtgataggt gaaaaacctg
     8101 cccccgccaa actgggaagg cagttcctgc ataggcagat tgttgtagga acccatcatg
     8161 aagtcgtacc aggattcccg aatcaaggta ggctggatgg acacatcata ttccggtggc
     8221 aaggctgcca gacaggctac cgcggtcaaa accgcgcaaa gcagagcgag cttcttcatt
     8281 gattcctccc atgatccaga tgatcaagta caagtttgcg caacttagcc agggtgtcaa
     8341 gtaaaataat cccgcacata actccctttg tggaatggca gttgcatata agcgccaaca
     8401 cccaattcag agcttatcct ggagaataaa acatgcgcaa aaccgcagtc acaattacgc
     8461 ttatcatcat gctcagcctc ttgctggctg atctgatcaa ccccgccggg tccgtcatct
     8521 ttggagacct gcgcgataca gacggcagcc tctccttcag cgtgaggacg gccacctaca
     8581 acggagccta cgcgccccgc aacgctggcg tgatctggat caccaactct caaaaccagt
     8641 tcatcaagac catcaagatc tgggccaaca gctatcgcta caccctcatc cgctggatcg
     8701 ccagttcggg ccaaaacacc accggggccg tcaccagcgc ttcctacaac aaccaccagc
     8761 ttcacaacgt gacctggaac ggtaaaaact atcagaatca ggacgttccg gacggcgaat
     8821 acaagatcaa cgtggaattc acggagcaca atgccaatgc caacaacctg ggcaaattca
     8881 agcaggtgac ctttgtaaaa ggaccggacc cgatcgatat caccatcccc aacgagagct
     8941 atttcagaga tatgcacctg gtctggcagc cggtgatcca aaacggcatt atctccggct
     9001 acgtgaggga cgagagcaat cagcccattc agggggctat catccaggcg ggagctttca
     9061 gcaccagcag tcagtccgac ggtcactaca gcctcaccgt tccgcctggc acctacacgg
     9121 tggtgtgtct caacacggga tatcaggata tcatcgccac agacgtggtg gtaacctcca
     9181 atcagactac cacacaggac tttatcatga tgacggtggc caacgacgat ccactctcac
     9241 cagccgcatc tttgagcctt aaagctcctt atcccaatcc tttccatcag gagactacaa
     9301 tccggatatc acttcccaaa gcagttccct ataccataga gattttcaac cttagaggtc
     9361 agaagctcat ctcccgccag atgatccctc aaagcaaagg agaacagcag cttagctggg
     9421 atgggcggga tatgtttggc aagccctgtc cgcctggaat ttatcagatc agcatcaatc
     9481 agggaactca gaaaatctgc accagagtga ctttacgctg aggatggttt agtttcccgc
     9541 agatttcgca gattggcacg ctgatttcgc agattggggg
//
LOCUS       DAOSFN010000097         9565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6082_length_9565_cov_7.671399, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000097 DAOSFN010000000
VERSION     DAOSFN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_9565_cov_7.671399"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..697)
                     /gene="tsf"
                     /locus_tag="PLO35_06945"
     CDS             complement(101..697)
                     /gene="tsf"
                     /locus_tag="PLO35_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOH79441.1"
                     /translation="MSEINATMVKELRERTGVGMMECRKALVETNGDMDAAIKFLRER
                     GATKAEGKASRETKEGVIHSYIHFNGKIGVMIELNCETDFVARTDQFKALADEICLQI
                     AATNPLAISADRIDPAVIEAEKDIARNKAILDGKKAEIVDKIVEGHIKKYCSEHSLLE
                     QELISDDKRTVKNLITEAITTTGENIQIARFARFELGE"
     gene            complement(763..1566)
                     /gene="rpsB"
                     /locus_tag="PLO35_06950"
     CDS             complement(763..1566)
                     /gene="rpsB"
                     /locus_tag="PLO35_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015562572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOH79442.1"
                     /translation="MSVVSMKQLLEAGVHFGHQTFKWNPKMRKYIFIKRNGIHIIDLK
                     QTVDAINEAYQFVKEVANRQEYILFVGTKKQAQGAIKEASDKAGVFYVNQRWYGGMLT
                     NMATIRQSIEKMKYYEEIVADGTISNYTKLEQQKMKRMHDKIEFSLGGIRDMDAVPGA
                     IFIVDTDYEKIAVHEARVLGIPIVGMVDTNCDPDLVDYVIPSNDDATRAIYLIADIMA
                     NAVIEGRNIASEGREQNAEEVTPAEGFAPETVEMAASAPEVEIPVLDGE"
     gene            complement(1741..2127)
                     /gene="rpsI"
                     /locus_tag="PLO35_06955"
     CDS             complement(1741..2127)
                     /gene="rpsI"
                     /locus_tag="PLO35_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOH79443.1"
                     /translation="MQNYDAVGRRKNAVARVRITPGTGKRIINKVQMKKYLQRETLEM
                     VVEQPLQELGLSENFDVHVNVRGGGLSGQAGAIRHGITRALVDYDEKLRPALKARGFL
                     TRDPRMVERKKSGRPKARKRFQFSKR"
     gene            complement(2131..2568)
                     /gene="rplM"
                     /locus_tag="PLO35_06960"
     CDS             complement(2131..2568)
                     /gene="rplM"
                     /locus_tag="PLO35_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOH79444.1"
                     /translation="MKTLTPSPKDIRQDWYVVDATGLALGRLSTQVASILRGKNKPYF
                     APNLDTGDYVIVINAEKVRVTGLKAQQKIYKTFSGYPSGLKEIPYARIMEKHPERIIE
                     HAVKGMLPKTVLGAAMFKKLKVYAGTEHPHTAQQPVVLDLKEK"
     gene            complement(2676..>3221)
                     /locus_tag="PLO35_06965"
     CDS             complement(2676..>3221)
                     /locus_tag="PLO35_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOH79445.1"
                     /translation="TGSLAGIVRSAGFAPILGALIQAGQYQSTTNGAGAYNLVLPIGT
                     YEVTASAPGFISSTQTDVLISESLVTTLNFYLEPGSAIDDPSIPPVKTSLTGIYPNPF
                     RQHCSLELELAKSCRIKLCIYDIRGRRVAILKNEQLPAGAHQISWQAVDASGRALPSG
                     VYILRMEAGPEVFIRKLMLLK"
     assembly_gap    3222..3321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3322..7453)
                     /locus_tag="PLO35_06970"
     CDS             complement(<3322..7453)
                     /locus_tag="PLO35_06970"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOH79446.1"
                     /translation="MSRPIVIVLLLLALLPAFALEASESPRQNSDVQLRILDLRGNNT
                     PSLDTPCVYIVKVINEGTADCTGFYATVNINYSKSIGQSESYSLAAGAMMEVPVPCMF
                     ELSGSLNYLRAILYIDSQSEPVTDQNVKLRADTSSIIYTFAAYSKDDHDSEAPLDLRY
                     KSNLFQCVFLESEIRQTGRITALRIFKRFGYSSVTRTIKIWLGHTDQSELTDGFISPH
                     LQSLVFDGSLTISTGFGMMGATLHTPFDYQGGNLVMTILMPWADVPLQNSNVFEGLVN
                     PGIRYRQARSSTQAINPYDPPQTSYSGGFVPLTNFGFSAYLPDAVAVITPTDHAFGRI
                     VQGNNPSQSFSIMNQGQAALEISDIQIIGSPAFSLKALPPLPMTLPMSRRIEFAVHVD
                     GEEIGDKHATLLLRGPGGVVLGSAALQAEIETQEYSLPLMQDFSWAQGYELPHGWEMI
                     NHSTASTAAIGSTQNQGCQTPGAAFMFNGLDGNAPLYIVAPAFSSSASVQAFRLKFWA
                     KALWPVTLQLGLIPANLDATEFTPKACYDLETLWNGYWIDIARPPDGFRIAFRHGGGV
                     YSDYIWLDDISVAAIPLHDLAVQQLGGPSQPLLGALARYQIEVKNWGAVNENDYQVQL
                     MDQNENILWQGSGPALQALGIQVIEASWYPSDSGPMTLKARVRLSGDEATWNDLSQPY
                     QIDVVQADNAYLLLGENRRQENLPFNFNSNNSLYEVLIPHAQMQSFYGQITGISLSGS
                     FVNPMNNKPLKIWMGSTGRDDLSAGWIPASQLNLVYDANLSIVPGPQALSFSFDEAFD
                     YRDGRNLVIMFNRAWDVLSYGASNRFQSWNIPSGLALGASSSSLTIDPALPIEGTPLG
                     YCPLIRFELQYGGVGHLIGRVVDHYSQALANAQISVNGSQDETLSDASGYFHLENLLC
                     GSNAISISKHGYRPFTWTVEIPDGGTLASEYRLSSYPQINIQGRILASDTQSPIYEAR
                     IRIWGYDEAETLSGGDGGFQFVNIWGEHSYSFSITASGYAPLNGVWDLGNQDLSLGDL
                     VLSELAWPAHTVMAVEDSLDGVVNLSWQPPSEQASEITEGFESDSFPPPQWEQVITNP
                     DPPGPSGIPPTWSSFGPVSIDGTGVNPPSGTRQAGLWWSFNHQDEWLISPLFNCPPEA
                     LLSFETWCRYGSNAGDSYSVKLSNDLGQNWITLWNASDLEPGWNQYQTPIQIDLSPWG
                     GSAVKLAWHALDPPSNDGLWYVWFIDAVSVSNTSKTLKFFTDEVSGSRGTSARSRKSN
                     QLSGNGPAAKHIAESLPRSYGSREHTGYRIARGFRAVESSSETWTDLAELPISQIDYT
                     DPDWGSLADGLYRWSVTAIYSNGVVASPAISNAITRHNITGSLAGIVRSAGFAPILG"
     gene            7691..8743
                     /locus_tag="PLO35_06975"
     CDS             7691..8743
                     /locus_tag="PLO35_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79447.1"
                     /translation="MKKLFITLVFTGLMLSLTANPIILKAIARVWFNDLDEFIVLLSD
                     ESVTYGMPIPFEDLVFSTSSGVWQFPDGFSVPGVPYQVNLSQVIPGFTIDKNADQLLV
                     QCDGGPGAYEMIRWDNLIDPAPDIRPLLTAQAAVQFRVVGNMMWSVDIWGKDNVQYHL
                     PAHEVVARCMINVHVQDYTGNPFEGLEVVRSYQNYIEQNYYHQFYTDENGNVSMESFP
                     CRIRIGVKDPVTEAWVLDESVYCEPGETYNYTATVTHTDTDDHVLPVVQSLSIWPSVM
                     GSQSSNFLKLKGPALTSEGELRLYDLRGRYLCSQIMPITGETQWRLPDLESGIYFVKL
                     VQGGQNLGSARFTVLK"
     gene            8740..9333
                     /locus_tag="PLO35_06980"
     CDS             8740..9333
                     /locus_tag="PLO35_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79448.1"
                     /translation="MKRIWLAILLLVWLGLAAQNAGFNFEGEKLEFHLRPDAKTGELI
                     WELDGVYWFSNLSEEPLSQVIAFPVPSDSLCSEAQDLSLKIGSYGDSTYVNLLNQWPQ
                     GFSFRLEAPQRRLVAVQISYHQRLQGKTARYVLMTTNSWGRALPLSEISLRVDKSLRV
                     KNISLSDYQLESLEGDALFTWSLSDFVAATDLIVELE"
BASE COUNT         2030 a         2452 c         2696 g         2287 t
ORIGIN      
        1 gagctccggc cagcacttct ccgctgagtt tcagcaggat acggtggatc ttttcgggct
       61 ggtaggtctg gctgttcatc actgcctgta tatgagtcgc ttattcgccc agttcgaaac
      121 gggcgaatct ggcgatctgg atattttcgc cggtggtggt gatggcttcc gtgatcaggt
      181 ttttcacggt gcgcttgtcg tcactgatca gctcctgctc cagaagggaa tgctcgctgc
      241 agtacttttt gatgtgtcct tccacgatct tatccacgat ctcggctttc ttgccgtcca
      301 gaatagcctt gttgcgggca atgtcttttt cggcttcgat gacggcgggg tcaatcctgt
      361 cggcagaaat ggccagggga ttggtggcgg cgatctgaag gcagatctcg tcggccaggg
      421 ccttgaactg gtcggtgcgg gccacgaagt cggtctcaca gttgagctcg atcatgacac
      481 cgatcttgcc gttgaagtgg atatatgaat ggatgacgcc ctctttggtc tcgcgggaag
      541 cttttccctc ggccttggtg gcaccacgct cacgcaggaa tttgatggcg gcgtccatgt
      601 cgccgttggt ctcgaccagc gctttgcggc actccatcat gccgacgccg gtgcgctcac
      661 gtaactcttt taccatggtt gcattaatct ctgacatggt tttctccttg tgtcaaagta
      721 ggggagccgc atacgggtcc cgcatgcgag ctcccttaaa tattactctc cgtccaggac
      781 ggggatttca acttcgggtg cggaggctgc catctccacg gtctcggggg caaagccctc
      841 ggcgggagtg acttcctcgg cgttttgctc tctgccttcg gaggcgatgt tgcggccttc
      901 gatcacggcg ttggccatga tgtcggcaat cagatagatg gcgcgggtgg catcgtcgtt
      961 ggaggggatc acgtaatcca cgaggtcggg atcacagttg gtatccacca tgccaacgat
     1021 cgggatgccc agaacgcggg cttcatgcac cgcgatcttc tcataatcgg tgtcaacaat
     1081 aaatatagcg ccgggaacgg cgtccatatc acgaatcccc cccagggaga attctatctt
     1141 gtcgtgcatg cgcttcatct tctgctgttc gagcttggtg tagttgctga tagtgccgtc
     1201 ggcgacgatc tcttcgtaat acttcatctt ttcgatgctc tgacggatgg tggccatgtt
     1261 ggtgagcatg ccgccatacc agcgctggtt cacataaaag acgccggctt tatcagaggc
     1321 ttctttgatg gcgccctggg cttgcttctt ggtgcccaca aagaggatgt attcctggcg
     1381 gttggccact tcttttacaa actggtaagc ttcattgatg gcatcgaccg tctgtttgag
     1441 gtcgatgata tggatcccgt tgcgcttgat gaagatatac ttgcgcatct tgggattcca
     1501 tttgaaggtc tgatgcccaa agtggacacc ggcctcgagc agttgcttca tagaaactac
     1561 tgacatggtg tgttctcctt aagatgatac ggttgatttt ccggacgcgg atctaaacaa
     1621 ttcagaggga gcctgtccct caccgccttg caactccgcg agcggtttga tgggtttgcg
     1681 tgcagctgtt tattcatttc agcaaggggg aagcaggttt tgcttcccca aaacccggga
     1741 ttaacgtttg gagaattgga atctctttct ggctttcgga cggccgcttt tcttgcgttc
     1801 caccatacgg gggtcgcggg tcagaaaacc acgggctttg agggcaggtc tcagcttttc
     1861 atcatagtca acgagagctc tggtaatgcc atgacggatc gctccggcct ggccgctgag
     1921 gccaccaccg cgaacgttca cgtggacgtc gaagttctcg ctcagtccca gctcctgaag
     1981 gggctgttcc accaccatct cgagagtctc gcgctggagg tatttcttca tctggacctt
     2041 gttgatgatg cgctttccgg ttccgggagt gatccgaacg cgggccacgg cattctttct
     2101 tctgcctacg gcatcataat tttgcatgtt ttacttctcc ttgaggtcaa gcacgacggg
     2161 ctgctgagcg gtgtgaggat gctcggtgcc ggcgtaaact ttgagttttt tgaacatcgc
     2221 tgctcccagg acggtcttcg gcagcatgcc tttaacggcg tgctcgatga tcctttcggg
     2281 atgcttttcc atgatccggg cgtaggggat ttccttgagg ccgctgggat atccgctgaa
     2341 ggtcttatag atcttctgct gggctttgag accggtcaca cgcacctttt cggcgttgat
     2401 cacgatcaca tagtcccccg tgtcgagatt cggcgcgaag taaggcttgt tcttgccgcg
     2461 cagaatcgat gcgacctggg tggataagcg tcccaaggct aaacccgtgg cgtccacgac
     2521 ataccagtcc tgccggatgt ctttgggact cggtgtgagg gtcttcattg ggtctccttg
     2581 tacggttttt tattcagaac gccagaattt ttgccccggc ttccatgtca agcaaaaagg
     2641 taagggattg agatttacgg gtattagggg agtgactatt ttagcaacat aagtttgcgg
     2701 ataaagacct caggtccagc ttccatcctg aggatataaa ctccggaggg gagcgctctg
     2761 ccagatgcat ccacagcttg ccacgagatc tgatgcgctc ccgctggaag ctgctcattt
     2821 ttgaggatgg cgactctacg cccccggatg tcataaatac atagctttat acggcaggat
     2881 ttggccagtt ccagttccag gctgcagtgc tggcggaagg gattgggata gatgcctgtg
     2941 agggatgtct ttacaggtgg tatggaagga tcatcgatgg cgctgccagg ttcgagatag
     3001 aagttgaggg tggtcaccag gctctctgag atcaggacat ccgtttgagt ggagctgata
     3061 aatccgggag cgctggctgt gacttcatag gtcccgatcg gcaacacgag gttgtaggcc
     3121 ccggccccgt ttgtggtgga ctgatactgt cctgcctgaa tgagggcacc caggatggga
     3181 gcaaatccgg ccgacctcac tattccggct agtgagccgg tnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn ncacccagga tgggagcaaa tccggccgac ctcactattc
     3361 cggctagtga gccggtgatg ttgtgccgtg tgatagcatt agagatagcg ggagaggcaa
     3421 ctacgccgtt ggaatagatg gcggtcacac tccatcgata gagcccgtct gccagcgatc
     3481 cccaatccgg atcggtgtaa tctatctggg agataggcag ttccgccaga tcggtccagg
     3541 tctcagagga gctttccaca gcccggaagc ctcgggcaat gcggtagccg gtgtgctctc
     3601 tgcttccgta actcctggga agggactcag ctatatgttt tgccgcaggg ccgttgccag
     3661 ataactgatt gcttttacga gatcgagccg atgttcctct gcttcctgaa acctcatcag
     3721 taaagaactt cagggtcttg gaggtattgg acacgctgac cgcgtcgata aaccacacat
     3781 accagagccc gtcgttggaa ggaggatcga gagcgtgcca ggccagcttg acagcgctgc
     3841 cgccccaggg agaaagatcg atctgaatgg gggtctgata ctggttccag ccgggttcca
     3901 gatcgctggc gttccagagg gttatccagt tctgccccag atcgttactg agcttgacgc
     3961 tgtagctgtc tccggcattg gagccgtagc ggcaccaggt ctcaaagctg agcagggctt
     4021 ccggagggca attgaaaagc gggctgatca gccactcatc ctgatggttg aagctccacc
     4081 agaggccagc ttgcctggtt cccgagggag gattcacccc agttccatcg atgctcacag
     4141 gtccaaaaga actccaggtg ggcgggattc cggaaggtcc gggtgggtct ggattggtga
     4201 tcacctgttc ccactgcgga ggtggaaagc tatcggattc aaagccttcc gtgatttcgg
     4261 atgcctgctc actgggaggc tgccagctga ggttgaccac gccatcgagg ctgtcttcca
     4321 cggccatgac ggtgtgggcc ggccaggcca gttcactgag gaccaggtct cccaggctca
     4381 ggtcctgatt acccaaatcc caaactccgt tcaggggtgc atagccgctg gccgtgatgc
     4441 taaagctata gctgtgctca ccccagatgt ttacaaattg aaagccgcca tcccctcctg
     4501 aaagtgtctc tgcctcgtca tagccccaaa tcctgatcct ggcttcgtat atggggcttt
     4561 gagtgtcgct ggccagaatc ctgccctgga tattgatctg gggataagag ctcaggcggt
     4621 attcacttgc cagagtaccg ccatccggga tttcgacagt ccaggtgaag ggacggtagc
     4681 cgtgtttgga gatgctgatg gcattgcttc cgcagagcag gttttccaga tggaagtagc
     4741 cgctcgcatc gctaagtgtc tcatcctgag agccgttgac ggatatctga gcgttggcca
     4801 gcgcttgcga atagtgatcc accaccctgc caatcagatg gcccacgcct ccatattgca
     4861 gctcaaaacg gatgagagga caatagccca gcggagttcc ctcgatgggc aaggcaggat
     4921 cgatcgtgag gctggacgag ctggctccca aagcaaggcc ggaagggatg ttccagctct
     4981 gaaagcgatt gctggcgcca tagctcaaca catcccaggc ccggttgaac atgatcacca
     5041 gattgcgccc gtcccgatag tcgaaagcct catcaaagct gaagctgagg gcctgaggtc
     5101 caggcacgat gctgaggttg gcatcataca cgaggtttag ctggctggcg ggaatccagc
     5161 cagcgctgag gtcgtctctg ccggtgctgc ccatccatat ctttaacggt ttgttgttca
     5221 tggggtttac gaagctgcca gagaggctga tgccagtaat ctggccatag aaagactgca
     5281 tctgagcatg cgggatgagc acttcgtaga ggctgttgtt ggaattgaag ttgaagggca
     5341 ggttttcctg acgccggttt tcacccagca gcaaataagc gttatcggct tgcaccacat
     5401 cgatttgata aggttggctg aggtcattcc aggttgcctc gtcaccactc agacgcactc
     5461 tggccttcag agtcattggt cctgaatcgc tgggatacca ggatgcctca atcacttgaa
     5521 ttcccagcgc ttgcagagcc ggaccgctac cctgccagag gatattttcg ttctggtcca
     5581 tcaactggac ttgataatca ttctcgttca cagctcccca gttttttacc tcgatctgat
     5641 accgggccag ggctcccagc aagggctgag acggcccccc gagctgttgg acggccagat
     5701 cgtggagggg aatggcggcg acgctgatat catccagcca gatataatcg gaatagaccc
     5761 caccgccatg ccggaaggcg atccggaaac catcgggagg cctggcgata tctatccaat
     5821 aaccattcca taaggtctcc agatcgtagc aggctttggg ggtaaactct gtggcgtcga
     5881 ggtttgcggg gatgaggcca agctgcaacg tgacgggcca gagggccttg gcccagaact
     5941 tgagcctgaa agcctggacg gatgcggagg agctaaaggc cggggccaca atgtagagcg
     6001 gcgcgttgcc atccaggcca ttgaacataa acgccgcccc tggagtctgg cagccctggt
     6061 tttgggtgct gccaatcgcg gcggtgcttg cggtgctgtg gttgatcatt tcccagccgt
     6121 ggggaagctc gtatccctgg gcccagctga agtcttgcat cagcggtaat gagtattctt
     6181 gcgtctcaat ttcagcctgc aaagcagcgg aacccaggac aactccaccg ggacccctta
     6241 acagcaacgt cgcgtgtttg tcgccaatct cctctccatc cacgtgtacg gcaaactcta
     6301 tcctcctgga catcggcagc gtcattggca agggcggcaa tgctttaagc gagaaagccg
     6361 gggaacctat aatctggatg tcagagatct ccagagcagc ctgcccctga ttcattatgg
     6421 aaaagctctg cgacggattg ttgccctgga caatcctgcc aaaagcatgg tcggttggag
     6481 tgataacagc aacggcatca ggcagatagg cactgaagcc aaagttcgtg agcggtacga
     6541 aacctcccga atagctggtt tggggagggt catagggatt gatagcttgt gtgcttgacc
     6601 gggcctggcg atatctgata ccgggattta ccaggccctc aaacacattg ctgttctgca
     6661 gaggaacatc agcccagggc atcaggatcg tcatcaccag attgccgccc tggtaatcga
     6721 aaggagtgtg cagggtcgcg cccatcatgc caaatccggt ggaaatggtg agtgatccat
     6781 caaacacgag gctctgcagg tggggactga tgaagccgtc ggtgagttct gattgatcag
     6841 tgtgccccag ccagattttg atggtgcggg ttacactgct gtacccaaag cgcttaaata
     6901 tcctcaaggc cgtaatcctg ccggtttgcc ggatctcgct ctccagaaag acacactgga
     6961 agaggttgct cttgtaacgc aggtccagag gggcttccga atcatggtca tctttggaat
     7021 aggctgcaaa agtgtagatt atactgctgg tgtctgccct cagcttcaca ttctgatcgg
     7081 tcactggctc gctttgagag tcgatgtaaa gaatggcgcg gaggtaattc agactgccgc
     7141 tcagttcaaa catacaaggc acgggaactt ccatcatggc cccggctgcc agagagtagg
     7201 attcagattg accgattgac ttgctgtagt tgatgttcac tgtggcgtaa aagcccgtgc
     7261 aatcggcagt tccctcgttg atcaccttca cgatatacac acagggagta tccagtgaag
     7321 gagtgttgtt gccgcgcagg tccagaatgc ggagttgcac atcgctattc tgacgcggtg
     7381 actcacttgc ctcaagggcg aaggctggca gcaaagccag cagtagcagc actatgacta
     7441 tgggtctgga catatctcct ctgcggatat tgatcgtgaa gataagaaac aatttcgctg
     7501 cctgactgga tcgtatttca ctttcctgag ccaggcatag ctgtcaagtg aaagtttcag
     7561 tcccggtatt actatgcgta gaggcttgct cttttatctg tccatggaac ggaatatgct
     7621 tcagtaacgg aagtgacagg gtgtcggtaa gccaatgtca tgtggcatat accgcccccg
     7681 gggagtgaag atgaaaaagc tgtttataac gctcgttttt accggcctga tgctgagcct
     7741 gacggccaat cccatcattc tgaaggccat cgcgagggtg tggtttaacg atctcgacga
     7801 gtttatcgtg ctgctcagcg acgagagcgt cacctacggc atgcctattc cttttgagga
     7861 cctcgtgttc agcacctcga gcggggtgtg gcagtttccc gatggcttca gcgtcccggg
     7921 agttccctat caggtcaatc tctctcaggt gatcccgggt ttcactatcg acaaaaacgc
     7981 cgatcagctt ctggtccagt gcgatggcgg ccccggagcc tatgaaatga tcagatggga
     8041 caatctgatc gatcccgccc ccgatatccg tcctctatta accgctcagg cagctgtgca
     8101 attcagagtg gtgggcaaca tgatgtggtc tgtggacatt tggggtaaag acaacgttca
     8161 gtatcacttg ccggcccatg aggtcgtagc ccgttgcatg atcaacgtcc atgttcagga
     8221 ttataccggg aatccgttcg agggtttgga agtggtgcgc tcctatcaga actacatcga
     8281 gcagaactac tatcatcagt tttacacgga tgaaaacggc aatgtgagca tggaaagctt
     8341 tccctgccgc atcaggattg gcgtgaaaga tcccgtcacc gaggcctggg tgctggacga
     8401 gagcgtatat tgcgaaccgg gagagaccta caactacacg gccaccgtca cccacaccga
     8461 taccgatgac cacgtactgc ctgtggttca aagccttagc atctggccca gcgtgatggg
     8521 aagccagtct tcaaacttcc tcaagctcaa aggcccggcc ctcacatctg aaggagaact
     8581 gcgactttat gacctccgag gcaggtactt atgctctcaa atcatgccta taacaggtga
     8641 gacacagtgg cggcttcccg atctggagag cgggatctat tttgtgaaac ttgtgcaggg
     8701 cggtcaaaac ttgggatctg ccagattcac agtactgaaa tgaagcgtat ctggctggct
     8761 atcttgctgt tggtctggct gggcctggct gctcagaacg ccggtttcaa ctttgagggt
     8821 gaaaagctgg agtttcacct gaggccggat gcaaaaacgg gtgagctgat ctgggaactg
     8881 gatggcgtgt attggtttag caatctcagt gaggaaccgc tcagccaggt gatcgccttt
     8941 ccggtaccgt cggactcttt gtgttccgaa gctcaggacc tgagcctcaa aataggcagt
     9001 tatggcgaca gcacttacgt caatctgctc aatcagtggc cgcaaggctt ctccttccgt
     9061 ttggaagccc cgcagcgcag acttgtggcc gtccagatat cctatcatca acgcctgcaa
     9121 ggcaaaactg cccgctatgt actgatgact accaattcct ggggccgggc cctgccactc
     9181 agcgagatca gcttaagagt ggacaagagc ttgcgggtaa agaatatatc attgtcagac
     9241 tatcagcttg agagcttgga aggtgacgcc ctcttcacct ggtccctcag cgactttgtg
     9301 gccgccactg atctgatcgt ggaactggag tgagtcccga gctggcgtcg aattccaatt
     9361 cgacactgcg tttaacgcat aacgcgtaac gcgtaacgta tgaaaacacg gagaaaagca
     9421 gaggttttcc tgagaaaagc agagaatctg tttactatga gatatgagaa aatatgagcc
     9481 aatttggaat ggcaaacgtc tcgtttgccg tgcttggtct cgcctagaga ctatctcatc
     9541 aaaggaacag agtctattga ttagc
//
LOCUS       DAOSFN010000098         9391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6222_length_9391_cov_8.647708, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000098 DAOSFN010000000
VERSION     DAOSFN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6222_length_9391_cov_8.647708"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..3129
                     /locus_tag="PLO35_06985"
     CDS             223..3129
                     /locus_tag="PLO35_06985"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH79449.1"
                     /translation="MRRTIIFLAILLSLAGLGAETWYLASFNTPELMDREASTLREAV
                     RALDIRGLEVYHYNSHKVLAGIPESKASQFANATRLASLPLAGKLYLISRLDSSPFQI
                     SDEIGEVLLDMDDALVLRSNLTDVRLRELISYPFTELELIPLRFAQDGMNLSPAALDR
                     TDINQMLALINATSVQGMIQNLQDFVTRYALAPNRLQVAEWIRDKFLSFGLTDVQLFP
                     FQWNNTTQYNVVATITGSIYPDQFIVVGGHHDSTNNSGDPYASAPGADDNASGSVAAL
                     EMARVMAQSGYVPRSSIRFVTFAAEEFGLWGAKAYSQWAQDNNLDIRLMMNHDMIANN
                     STPAPNWQVRLMPYDGSMDHSAYAAQVTENYTSLTTFYGSMNSGSSDSHPFWQRGYNV
                     IYYFEEEFCPWYHSSNDIVANIDPLYAAEVIRASTAVASTFADMPSAPGYLSVFDAGD
                     GQSLILNWAPVIDPTIVGYRIYWGSQLNNLSGPINVTASPYTLSGLTQGQVCYIGISS
                     VDSFGNESYRIYSSGVPLVVPQTPTGFIDWPEPAVIALSWDPNQEYDLASYRIYRSTE
                     AGVTGNLLTTVPYSITSYIDSDVTGGQHYYYYTLEAVDEDNNASLQTLQIRSRPMSMD
                     QGVLVVDETLNLGGSTPFQPTDAQADDFYDHTLSGIPHHQIDIEDLFESMRLADIGIY
                     SSILWHGNDLSNMEYPYTVTETLDYYVANGGNILFSVYQPSLAFELNSSYPASFASNT
                     FMNAVLGIGSVNYSNAARFRRAIPLLDGYPAIEVDPAKTNSSFINHIIRVEGMETDGQ
                     STALYDYGSDYEDDSPQGVLNGTRVGILNQRGSGKVLTLSFPLYNMYASQARALVRHV
                     FTGEFGETATPADDPVAPSLPTLVLDAPHPNPFSASTTLRLNIKDASLPVSVNIYNQR
                     GQLVRKLNPDSGLKSITLSWDGKDESGRQVGSGIYLVKASQGKESVNRKLVLVK"
     gene            complement(3432..4433)
                     /gene="argF"
                     /locus_tag="PLO35_06990"
     CDS             complement(3432..4433)
                     /gene="argF"
                     /locus_tag="PLO35_06990"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOH79450.1"
                     /translation="MAFNLRNRHFLTLMDFSTKEIQFLLDLSLELKKAKYAGYEQPRL
                     SGKNIALIFEKDSTRTRCAFEVAALDQGAHVTYLGPTGSQMGKKESIADTARVLGRMY
                     DGIEYRGYGQEIVEELAKYAGVPVWNGLTDQDHPTQVLADFMTAMEHLHKPLNEMVFV
                     YAGDGRNNVANALMIGGAKMGMDFRIVAPKSLSPDAKLLKKCKDVAKETGATITVTDD
                     ITKGVKGADVIYTDVWVSMGEPDSVWASRIKLLKPYQVNADMMKKTGKKTTLFMHCLP
                     AFHDTKTVIGKQIFEKYGLNGMEVSDDVFESTHSVVFDEAENRMHTIKAVMVATLGC"
     gene            4701..5948
                     /locus_tag="PLO35_06995"
     CDS             4701..5948
                     /locus_tag="PLO35_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH79451.1"
                     /translation="MKRLMSLLIMALPLILMGQQYNLNDLIQQGMEQSWTTQRGRLSY
                     ESSASQLSSATWELLPDADLGLRINQNLHNQTPAADLTSSFNFSVSKTISLNDPAWNN
                     YRQAGITKAKAALNLQTSNSAYAYQVFDAYIKVLSAQKQLKSLTENLAIQTRVWEQAK
                     VLRQLGKNTDFDVKQSEIAVMNSRISIMQLENTIRSNRQSLFGLVQIADEGFPLADLE
                     PDKEFQVPELNPEAVNEIKLLKAEIKSARLTRNQSRLDFLPRLSLGYGLSRNISGDNF
                     EFDTYSTTHNIYLNLSYSLWNQFTQHQSSKRSDISYRLAELELMDKTDELGRQYQNAT
                     QELEYLNRLDELYREKLEQSSQQIKIAEERYRLGLIELLELDKTRTDYIDADIAYNAN
                     LYQILAKQQNLNFLLSRPILGKW"
     gene            5964..7463
                     /locus_tag="PLO35_07000"
     CDS             5964..7463
                     /locus_tag="PLO35_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH79452.1"
                     /translation="MKKYLKYIIIAIVLIVLILGYTSCRKKKNRPEWRTEKLSYGDIR
                     EVVTATGSLNPYVLVEVGTEVSGKIEKLYKDYNDTVKKGELLAKLDTELLRTSLESSK
                     ADLAKAQTSAQEAKLDMDNLKELVKRNMAAEYDLKKAEFKHQQAQQSLATAKLNLQRA
                     QKNLDNAFITSPIDGVIVARSVDEGQTVAASLNAPTLFKIANNLDKMQITANVDEADI
                     GKIQVGLPVEFNVDAYPAMPFRGEVQQIRLNPSTEQNVVSYSVIIDADNPGRVLLPGM
                     TTNVTIVIQSKQNVMRIPETATRFRPSKEVWELFGFKWDDDLISKAREKAMEELKKQM
                     EAARGAEPEAAPAQSVAKQTERGQVPGDQAQNPRQRPQTENKTPAKGDSTARRPGGRP
                     GMNPGQMPSGPGAQTASRPAGMPSGGAGFARGAMGFGRGGGMSMVWVLKGDKPELRMV
                     RTGISDGAFVEVLSGIKEGETLIGGVTYKNAKQAAAQNPMQGGPGMRRF"
     gene            7467..8225
                     /locus_tag="PLO35_07005"
     CDS             7467..8225
                     /locus_tag="PLO35_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79453.1"
                     /translation="MPDCSLIRTHKLSKTYQMGDFHVHALRGIDLCIGQGEFIAIMGA
                     SGSGKTTFMNLLGCLDYPSGGSYELDGIAVHEMPPEELTRVRNQKIGYVFQSFYLLPR
                     TTALENVELPLLYTRDLSHKDRVDKAMQALEVVSIADRARHFPNQLSGGQQQRVAIAR
                     AIVNDPAFLLADEPTGNLDTRTSIEVMALFQALHREGKTIVLVTHEFDISQYATRIIT
                     FRDGLIISDVPNSNPRNAIADLAKLPVLDDVEEA"
     gene            8226..>9391
                     /locus_tag="PLO35_07010"
     CDS             8226..>9391
                     /locus_tag="PLO35_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH79454.1"
                     /translation="MSVLGIIRIALKSLTRNKTRTFLTMLGVIIGVAAVITMLAIGEG
                     AKKIVDDQISSMGTNVIMISSNFMSSQSNVRQAQGGGNLLEIEDVQAVKDQVDGVLYA
                     SPVYNTWGQIKYESNNWRSGIMGVDVDYFLIRDISAASGDLFYSSDVENGAKVCVIGK
                     TVADNLFGDIDPVDKIIRIRNIPFTVKGVMSAKGENVMGMDQDDIVLAPYSTTYTRLL
                     GNRWRVMTMMVSADSKERIHLVQQDILNLLQGRHRGTTSEEFMVRTQTDIAETANSVS
                     NTMTILLASIAGISLLVGGIGIMNIMLVSVTERVKEIGIRMAVGASKRDVLLQFIIEA
                     VTISILGGILGILLGLGATSIVGKSMKWSVSVTPFSVLLSVSFSCAIGIFFGWYP"
BASE COUNT         2207 a         2748 c         2475 g         1961 t
ORIGIN      
        1 atgcaaaggg ggagccaaag ctgaaaaagg cgttaatcgc ctaaggatca tatacttgaa
       61 ttatgggcaa cctgatccgc ctggatagga ttatatcaat ttgatatggg gactgatatg
      121 atgtgcgcct cgttcgcgtg acttcggatt cgtcagggaa taaaacctgc tttatgatat
      181 gtgtataatc tgatcaaata acggaccaca aaggatagat ccatgagacg cacaatcatt
      241 tttctggcaa tcttactcag cctggccggc ttaggtgccg agacctggta tctggccagc
      301 ttcaacaccc cggaactgat ggaccgggag gcatcgactc tgcgggaggc cgtcagagct
      361 ctcgatatca gaggacttga ggtgtatcat tacaacagcc ataaggtcct ggcgggaatc
      421 ccggagagta aggcttccca gtttgccaac gccacccggc tcgcgagtct tcccctggct
      481 ggaaagctat atctgatcag ccgtctggat tcctcgcctt tccagatttc agatgagatt
      541 ggggaagtgc ttctggatat ggatgacgct ctggtgctcc gcagcaatct gacggacgtc
      601 cgtctgcggg agctgatctc atatcccttc acggagttgg agctaatacc cttgcgcttc
      661 gctcaggacg gcatgaatct cagtccggcc gctttggacc ggaccgacat caatcagatg
      721 ctggctctga tcaacgccac ttcggtgcag ggcatgatcc agaacctgca ggactttgta
      781 acccgctatg ccttggcccc caaccgctta caggtggcgg agtggatccg ggacaagttt
      841 ctcagctttg ggctcacgga cgtgcaactc tttcctttcc agtggaataa caccactcaa
      901 tacaacgtgg tggccaccat caccggtagc atctatcccg atcagttcat cgtggtgggt
      961 ggacatcacg actccaccaa caactccggc gatccctacg cttccgctcc cggagcggat
     1021 gacaatgcca gcggctcggt agccgctctc gaaatggcca gagtgatggc ccagagcgga
     1081 tatgtgccgc gttcatcgat ccggtttgtg acttttgccg ccgaggagtt tggcctctgg
     1141 ggcgctaaag cttactccca gtgggctcag gacaacaacc tcgacatccg tctgatgatg
     1201 aatcacgaca tgatcgccaa caacagcact cccgctccca actggcaggt gagactgatg
     1261 ccctacgacg gttccatgga ccacagcgct tatgccgccc aggtcacgga aaactacaca
     1321 agcctgacca cattctacgg ctccatgaac agcggcagca gtgacagcca tcccttctgg
     1381 cagagaggct ataacgtgat ctattacttt gaggaggaat tctgcccctg gtatcacagc
     1441 tccaacgaca tcgtggccaa catcgatcca ctttacgccg cggaagtgat aagggcctcc
     1501 acggcagtgg cttccacctt tgccgatatg ccctcagcgc ctggttatct ctccgtcttc
     1561 gacgcgggcg acggacagag cctgatcctc aactgggccc ccgtaatcga tcccaccata
     1621 gtaggttaca ggatctattg gggttcccag ctcaacaatc tgagcggccc catcaacgtg
     1681 actgccagtc cctacaccct tagcgggctc acgcaaggcc aggtctgcta tatcgggatc
     1741 agctccgtgg acagctttgg caacgaaagc tatcgcatct actcttcagg agtgcctctg
     1801 gtggttccgc agacccccac tggttttata gactggccgg aacctgctgt catcgctctg
     1861 tcctgggatc ccaaccagga atacgacctg gcctcctacc ggatctacag atcgacggag
     1921 gcgggagtga ccggaaatct cttaaccact gtcccataca gcattaccag ctatatagac
     1981 agcgacgtga ccggtgggca gcattactac tactacaccc tggaagccgt cgatgaagat
     2041 aacaacgcca gcctccagac cctgcaaatc cgcagccggc ctatgagcat ggaccaggga
     2101 gtgttggtgg tggacgaaac cctcaatctg ggtggctcca cccccttcca acctactgac
     2161 gctcaggcgg atgacttcta cgatcacact ctcagcggta tccctcacca tcagatcgac
     2221 atcgaggatc tctttgagtc catgcgcctg gccgacatcg gcatctattc ctccatcctc
     2281 tggcacggca acgacctctc caacatggaa tatccctaca ccgtgacgga aacgctggac
     2341 tactacgtcg ccaacggcgg caatatcctc ttcagcgtct atcagcccag cctggccttc
     2401 gaactcaaca gctcatatcc cgccagcttt gcgtcaaaca ccttcatgaa tgctgtattg
     2461 gggatcggca gtgtcaatta ttcaaacgcg gcccgcttca gaagggccat tccgctcctg
     2521 gacggatatc ccgccattga agtggacccc gccaagacca actccagctt cataaatcat
     2581 atcattcgcg tggaaggaat ggagacggac ggacaatcca ctgccctcta tgactatggc
     2641 agcgattacg aggacgacag ccctcagggt gtcctcaacg gaacgcgggt gggaattctc
     2701 aatcagcggg gatcgggcaa agtgctcacg ctcagcttcc ccctctacaa catgtatgcc
     2761 agccaggcca gagcgctggt gcggcacgtc ttcaccggcg aatttggaga aaccgccacg
     2821 ccagcggacg atccggtggc accatcactg cccactctgg ttcttgatgc cccgcatccc
     2881 aatcccttca gcgcttccac cactctgcgt ctcaatatca aggatgccag cctgcccgtg
     2941 agtgtgaaca tctacaacca gcgaggccag cttgtccgta aactcaatcc ggacagcggc
     3001 ttgaagagca ttaccctgag ctgggacggc aaggatgaaa gtggccgcca ggtgggctcc
     3061 gggatctatc tcgtcaaagc cagccaaggc aaagagagcg tgaaccgcaa gctggtgctg
     3121 gtcaaatagc tcatataccc ccatcagatt atccaaggcc ggaagtttct gcttccggcc
     3181 ttgatcgttt tcccttagct gagatgaacc aagccgggac cacctcggac aaatgcgagt
     3241 tgggtccgag atggtctgag gatgctacgc cgcagtgaat cggggcggga ttggtgctct
     3301 ccgatttgct ctacgggtgc tctacgggtc gcacccgtag agcacccgta atgcacccgt
     3361 aataggatgc gggcgcaaaa aagccggagc tccagagagg catccggctt agatgattat
     3421 atcgctggtg cttagcagcc cagagtggct accatcacgg ctttgatggt gtgcatgcgg
     3481 ttttcggctt cgtcgaagac cacggaatgg gtggattcaa agacgtcgtc gctcacttcc
     3541 atgccgttga ggccgtattt ctcgaagatc tgcttgccga tcacggtctt ggtgtcgtgg
     3601 aaagctggca ggcagtgcat gaagagggtg gtctttttgc cggtcttttt catcatatcg
     3661 gcatttacct gatagggttt gagcagtttg atgcggctgg cccacacgct gtccggctcg
     3721 cccatggaga cccaaacgtc ggtatagatc acgtccgcgc ccttcacgcc tttggtgatg
     3781 tcgtcggtga cggtgatagt ggcgccggtc tctttggcca cgtctttgca tttcttgagc
     3841 agcttggcat ccggggagag ggatttgggg gccacgatcc tgaaatccat gcccatcttg
     3901 gcgccgccga tcatgagagc gttggccacg ttgttgcgtc cgtctccggc atacacaaac
     3961 accatctcgt tgaggggctt gtggaggtgt tccatggcgg tcatgaagtc tgccaatacc
     4021 tgggtgggat ggtcctgatc ggtgaggccg ttccagaccg gcacgccggc atatttggcc
     4081 agctcttcca cgatctcctg gccatagccg cggtattcga tgccgtcata catgcgtccc
     4141 agcaccctgg cggtgtcggc gatggattct tttttgccca tctggcttcc agtggggccg
     4201 aggtaggtga cgtgcgcgcc ctggtccagc gcggccactt caaaggcgca gcgggtgcgg
     4261 gtgctgtctt tttcaaagat cagggcgatg tttttgccgc tgaggcgggg ctgttcatag
     4321 ccggcatatt tggctttttt gagctcaagt gacagatcca gcaggaattg gatctctttg
     4381 gtgctgaagt ccatcagcgt gaggaaatgg cggtttctga ggttgaaggc catgatgtat
     4441 tctcctgtgt ttattttaaa tgctgatgcc agttttttaa aagggcttta aaaggcaagg
     4501 attttccttg ccggattgag gggcttcagg atattggcat ccgggatttg gctgaaatag
     4561 attgcgggat aagacctatc cggcagtatt agagttgata aggtggagaa gtatgaggcg
     4621 cgcgtccctt ccctctgaaa ccttctcaag tccctcaagc cctttgactt caagattgac
     4681 atacacataa aagaggaaac atgaaacgac tcatgtcact actgatcatg gctttaccgc
     4741 tgatcctgat gggccagcag tataacctca atgatctcat ccagcagggt atggaacaaa
     4801 gctggaccac ccagcgcgga cgcctcagct atgagagcag cgcctctcag ctctcaagtg
     4861 ccacctggga actgcttcca gatgccgatc tgggcttgag gatcaatcaa aacctccaca
     4921 accagactcc cgccgccgac ctgaccagca gctttaactt cagcgtcagc aagacgatca
     4981 gcctcaacga ccccgcctgg aacaattacc gccaggccgg catcaccaaa gccaaagccg
     5041 ctctcaatct tcagacatcc aattccgcct acgcctatca ggtcttcgat gcctatatca
     5101 aggtgctgag cgctcaaaag caactgaagt cgctcacgga aaacctggcc atccagaccc
     5161 gcgtgtggga acaggccaaa gtactgcggc agttgggcaa aaacaccgat tttgatgtca
     5221 agcagagcga gatcgccgtg atgaattccc gcatcagcat catgcaactg gaaaacacca
     5281 tccgctccaa ccgccaaagc ctctttggtc tggtgcagat cgccgatgaa ggttttccgc
     5341 tggcagacct ggagcccgac aaggaatttc aggtgccaga gctcaacccc gaggccgtca
     5401 acgagatcaa gctcctcaaa gcggagatca agagcgcccg gctcacccgc aatcagagcc
     5461 gcctggattt tctgcccaga ttgagcctgg gttatgggct cagtcgcaac atctctggcg
     5521 ataattttga atttgacacc tacagcacta cccacaatat ctatctgaac ctcagctact
     5581 cgctctggaa ccagtttacc cagcaccagt ccagcaagcg ttccgatatc agctaccgtc
     5641 tggctgaact tgaacttatg gataagacgg acgaactggg gcggcaatat caaaacgcca
     5701 cccaggagct ggaatacctc aaccgcctcg acgagctcta ccgcgaaaaa ctggagcaat
     5761 ccagccagca gatcaaaatt gccgaagagc ggtaccgtct gggcctgatc gaactgctgg
     5821 agctcgacaa gacccgcacc gattatatcg atgccgatat tgcctacaac gccaatctct
     5881 accagatcct ggccaaacag cagaacctca acttcctgct ctcccgacca atcctcggaa
     5941 agtggtaaag gctataaaca tccatgaaga aatacctcaa atacatcatc atcgccatcg
     6001 tcctgatcgt cctgattctg ggctacacca gctgccgcaa aaagaaaaac cgtccggagt
     6061 ggcgcaccga aaagctctcc tatggagaca tccgcgaggt ggtcaccgcc accggttccc
     6121 tcaaccccta tgtgctggtg gaagtgggaa ctgaagtttc cggcaagatc gaaaagctct
     6181 ataaagacta caacgacacc gtgaaaaaag gcgagctgct ggccaagctg gataccgaac
     6241 tgctgcgcac cagcctggaa tcctccaaag ccgatctggc caaagctcag acctccgcgc
     6301 aagaggccaa gctcgatatg gacaacctta aagaactggt aaaacgcaat atggccgccg
     6361 agtatgacct caaaaaggcg gaatttaaac accagcaagc ccagcaatca ctggccactg
     6421 ccaaactgaa tttgcaacgg gcccagaaga atctggacaa cgcctttatc acctctccca
     6481 tcgatggagt gatcgtggcc cgcagcgtgg atgaaggcca gaccgtggcc gccagcctca
     6541 atgcccccac ccttttcaag atcgccaaca atctggataa gatgcagatt acggccaacg
     6601 tggacgaagc ggacatcggc aagattcagg tgggcttgcc ggtcgagttt aacgtggatg
     6661 cctatcccgc catgcctttc aggggtgagg tgcagcagat tcgcctcaac cccagcaccg
     6721 agcaaaacgt ggtgagctac agcgttatca tcgatgccga caatcctggc agagtattgc
     6781 tgcccggcat gaccaccaac gtcaccatcg tgattcaatc caaacagaac gtgatgcgca
     6841 ttcccgagac ggccacccgc ttccgtccca gcaaggaagt ctgggagctc tttggcttca
     6901 aatgggatga cgatctgatc tccaaagccc gtgaaaaagc catggaagag ctgaaaaaac
     6961 agatggaagc cgcccgggga gcagaaccgg aagcggctcc cgcgcaatca gttgccaaac
     7021 aaaccgagcg tggccaggtc ccgggtgatc aagctcagaa tccccggcaa agacctcaaa
     7081 ctgaaaataa gacccctgcc aaaggtgaca gcaccgcaag gcgtcctggc ggcagacctg
     7141 gcatgaatcc tggtcagatg cccagtggtc ctggcgctca gacagcttcc cggccggctg
     7201 gaatgccatc cggaggtgct ggttttgcca gaggcgcaat gggctttggc agaggtggcg
     7261 gaatgtccat ggtctgggtg ctcaaaggtg acaaaccgga gctgcgcatg gtgcgtaccg
     7321 gcatctcgga cggagctttc gtggaagtgc tttccggcat caaggaaggc gaaaccctca
     7381 taggtggagt cacttacaag aatgccaaac aggccgccgc ccagaatccc atgcagggcg
     7441 gacctggcat gagaaggttc tgatcgatgc cagattgctc tctgatccgc acccataaac
     7501 tcagcaagac ctatcagatg ggagactttc acgtccacgc cctgcgcggc atcgatctct
     7561 gcatcggcca gggtgagttt atcgccatta tgggagccag cggcagcggt aaaaccacct
     7621 ttatgaacct cttaggctgt ctggactatc cctccggagg ctcttacgaa ctggacggca
     7681 ttgccgtcca cgagatgccc cccgaagagc ttacccgggt gcgcaaccag aagatcggct
     7741 atgtgtttca gtccttctat ctgctgcccc gcaccaccgc tctggaaaac gtggaactgc
     7801 cgctgttata cacccgcgac ctgagccata aagaccgggt ggacaaagcc atgcaggctt
     7861 tggaggtggt gagtatcgcg gaccgggcca ggcactttcc caaccagctt tccggaggcc
     7921 agcaacagcg cgtcgccatc gccagagcga tcgtcaacga tcccgccttt ctgctggccg
     7981 acgaacccac cggcaatctg gatacccgca ccagcatcga agtaatggcc ctctttcagg
     8041 ctctgcaccg tgaaggcaag accatcgtgc tggtgactca cgaatttgac atcagccagt
     8101 atgccacccg catcatcacc ttccgggacg gtctgatcat ctcggacgtg cccaattcca
     8161 atccccgcaa cgctatagcc gacctcgcca aactgccggt gctggacgac gtggaggagg
     8221 cctaaatgtc agttttagga atcatccgca tcgcccttaa gtccctgacc cgcaataaga
     8281 cccgtacctt cctcaccatg ctgggcgtga tcattggcgt ggcggccgtg atcaccatgc
     8341 tggccatcgg tgagggcgct aaaaagatcg tcgacgacca gatcagcagc atgggcacca
     8401 acgtgatcat gatctcatcc aacttcatga gctcccagag taatgtgcgt caagcccagg
     8461 ggggtggaaa cctgctggag atcgaagacg tgcaggcggt caaagaccag gtggacggag
     8521 tgctgtatgc ctccccggtc tataacacct ggggccagat caaatatgaa agcaacaact
     8581 ggcgcagcgg catcatgggc gtggatgtgg actatttcct gatccgcgat atcagtgccg
     8641 cctccggaga tctcttctat tcctcggacg tggaaaacgg cgccaaggtc tgcgtgatcg
     8701 gcaagaccgt ggcggacaac ctctttggcg atatcgaccc cgtggataag atcatccgca
     8761 ttcgcaacat ccccttcacc gtcaaaggcg tgatgagcgc caaaggcgaa aacgtgatgg
     8821 gcatggacca ggacgacatc gtgctggcgc cttacagcac cacctacacc aggctcttgg
     8881 gaaaccgctg gcgggtgatg acgatgatgg tatcagccga ttccaaagag cgtatccatc
     8941 tggtgcagca ggatatcctc aacctcctgc agggccgtca tcgtggcacc accagtgaag
     9001 aatttatggt gcgcacccaa accgacatcg ccgaaaccgc caacagcgtc tccaatacca
     9061 tgaccatcct gctggccagc atagccggaa tctctctatt ggtgggaggt atcggcatca
     9121 tgaacatcat gctggtctcc gtcaccgaac gcgtcaaaga gatcggcatc cgcatggctg
     9181 tcggagcctc caaacgcgac gtgctgctgc agttcatcat cgaagccgtc accatcagca
     9241 tcctgggagg catcctggga atcctgctgg gcctgggagc aacttccata gtgggcaaat
     9301 ccatgaaatg gagtgtgtcc gtgacaccct tcagcgtgct gctctcagtg agcttttcct
     9361 gcgccatcgg catcttcttt ggctggtatc c
//
LOCUS       DAOSFN010000099         9333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6292_length_9333_cov_10.136775, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000099 DAOSFN010000000
VERSION     DAOSFN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6292_length_9333_cov_10.136775"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..1053)
                     /locus_tag="PLO35_07015"
     CDS             complement(274..1053)
                     /locus_tag="PLO35_07015"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOH79455.1"
                     /translation="MIDSAFTLLDSKLPVLATAIHAGHQLGPEMDHHCGIPEADRFRE
                     EDPYTDEAAALFANHIILRSSRFEVDLNRLRDKAVYLVPEDCWGLPVSRQNLPPHIIA
                     SLYSEYDQWYAHLDFCIKRMLSHHPFIVVLDLHSYNHRRGGPNAQPDPQESNPDIIIG
                     RSNMSARYHKSVESLRARLDGQSWQGRKLDCRADVKFPGGYLARYLNAHYSDHLFCLA
                     IEFKKTFMDEWTGQLDRDAWDELKGLFKTAVDEWLPEVVRA"
     gene            complement(1050..1703)
                     /locus_tag="PLO35_07020"
     CDS             complement(1050..1703)
                     /locus_tag="PLO35_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79456.1"
                     /translation="MNTPRHDDLMSYLRDLRENLLQVAIYKGYDGVEHAILIFEDDPP
                     SFFDVLQPLVRVIRKRRLPEPLIVNRIFVENSLDSYPLEFLNMQTSYRNLLANTDILK
                     GLRFAKADLRLQMERELRSKWLLTRQAVLENPYKTAQVRDAIQRSRTAIHPVLKGFFA
                     LKDLSVPKELAEAIELATPISGVDLNPLQLTITGIQDASRYIAMLDALMTRVQGWVL"
     gene            complement(1700..2551)
                     /locus_tag="PLO35_07025"
     CDS             complement(1700..2551)
                     /locus_tag="PLO35_07025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOH79457.1"
                     /translation="MTFQDMILTLQNFWAQRGCALLQPYDCEMGAGTFHPGTFFGSLG
                     SKPTAIAYPQPCRRPKDGRYGENPNRFQHYYQFQVIIKPTPEDIQTQYLRSLEAIGVT
                     IGDHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVAGYDLFPVSVELTYG
                     LERLAMYIQDVDDYRELMFSDSVKYADLYFTREQEYSDYNFNSADTTLLFRLFQDHEK
                     ECNALLDKGLVYPAYDQLLRCSHYFNLLDARGVISVTERAAYIGRVRDLARLSARTYL
                     EKYEEKP"
     gene            complement(2548..2928)
                     /locus_tag="PLO35_07030"
     CDS             complement(2548..2928)
                     /locus_tag="PLO35_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOH79458.1"
                     /translation="MKYFYTIGEVSNLLGVKPHVIRYWESEFSFLKPRKEEGRIRKYS
                     EENVQLLRKIQEMLHTQRFTIEGARQKLKAERSAARYHRKLEELSPPVAISDPALKEQ
                     LGQLRSSLKDILTLCDKLKRKYKQ"
     gene            complement(2925..4451)
                     /gene="lnt"
                     /locus_tag="PLO35_07035"
     CDS             complement(2925..4451)
                     /gene="lnt"
                     /locus_tag="PLO35_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOH79459.1"
                     /translation="MKHKDLILTFASVLLLALARLPLHLGFLVFIGWIPLMIVLQEGG
                     RSFKRLLLMGLIWSLVYVGIVFYWISEVTWGGLIGIVGFYAVWLALFFYVIQRIWSRF
                     PAWGHLGFIAVMLSFEYLQNFGETRFPWWNSGYSLAAYPQLIQAADLGGMTLLALLAL
                     IINILVDKASRGKLRNLIWIALIMSAWIGYGQYCLISLQPQKRELRAQVMQPSIPQED
                     KWEQKHYPQMLARFEELSLKAAADSTQLFIWPEAAMPAYLRYDMDRQRDLREIINLTG
                     LEIFTGFPDFDSAPPEHVDEFYYYNGASLFKPDGSQQPVFHKNILVPVGERMLWLKQF
                     PILWKLNFGQANWEFGKELAYYESFGYRYSPSICYELAFPDIHHRMAMPKDPQTGRIT
                     KMDFLVNLTNDAWFGTSYGPWLHQVMTRFRAVENRIQIYRSANTGISVIVDPLGRELA
                     SAGLFKITNLGAPLYTVDKVPLIRHIHSYPLLFLILAAGLFLLSLFKAAAPPSKEASG
                     "
     gene            complement(4503..5300)
                     /locus_tag="PLO35_07040"
     CDS             complement(4503..5300)
                     /locus_tag="PLO35_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79460.1"
                     /translation="MAFLMLSSCASARFNPKREIEAVTFSEFIQSERAYDKAVADPVS
                     GSVFALLKAEQQIHVFREGKKSNSFGGLGTQAYNFQRLTDIALDNDGSLLALDMAAKE
                     LRRFSPDGNLLARVDLSSLSQPELLVMSPDRDLFIYDAAPQELICISMLDGSELYRFG
                     KFQLKSPSTLSCNRDLLSVYSQSADETQFYYILGQFKESRQGQWLQDAFGNWVLAELP
                     RGTVVEEASLMPLLAGSGVASVYRESIAVVYENGIGLYRIMHRRPEQ"
     gene            complement(5491..7209)
                     /gene="recJ"
                     /locus_tag="PLO35_07045"
     CDS             complement(5491..7209)
                     /gene="recJ"
                     /locus_tag="PLO35_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOH79461.1"
                     /translation="MEKEWITPPGLSAEEKQLVSELSDSLKSPPMVSELLFRQGLKTE
                     QEIRDFFTPSLYHQHDPFLFEDMDKAVTRIVQAIERHEKITIYGDYDVDGTTATALLL
                     LGLRKLGAEIDFYIPHRMIDGYGLSLGSLDQLKESGSSLIISVDCGVNALEEIAAINA
                     MGMEIIITDHHNPKEELPKALAIINPKLPGTRYPYPHLAGVGVAFKLLMAIYQRMGYQ
                     NVETTLKYMDLVAVGTIADIVPLTGENRVFASIGLQHLIDKKNLGLNALVSISGMNHK
                     TLDTNDIVFGIAPRINAAGRMGSAALAVELLISCEEAQSDELAEVIERQNSLRQQLDQ
                     RTFHEACEIIEKKYKDLANTPCMVISSDDWHPGVIGIVASKLVEKYYRPVIMISFKEG
                     FGSGSGRSMGDFDLFSALKHTEHNLHSFGGHKYAVGLTIYQEYIDRFENELTRYVSDT
                     LRTEQIKPPLQIDHELELYDINDFFLDWIERFAPFGPDNNRPVFATRGVCVASYPYNV
                     GRNHIKLKVMKDGMSLDLIGYNLGDYLSLLKKNSIVDIAYTLEYNRFGNKTSIQGKLR
                     DIHVRQ"
     gene            7837..>9333
                     /locus_tag="PLO35_07050"
     CDS             7837..>9333
                     /locus_tag="PLO35_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOH79462.1"
                     /translation="MNQRSCEDIYYQYRSQAINHPQTYLESLPSLKLKAQVPPMALAL
                     NSVEAWLVRGDFCKARQELDALAAEIKKTADPSLKSHWQLLRHSLMFQTSVDTQTAQE
                     ELDKAARYQVMSGSSALECEVLLHRCLQNMQPWEITQSEETLQKAMSAASKSGQQELV
                     LEVYLAFIQLYLTQNLVEQANRELLLIHDLIQPDLYPLKYLQLYNLRGIISNMTRSWK
                     EAAEHFKSGINLAEEHGYGYQLAQLWMNLGISLVSQRDFNGGIAMYDQSLAMLSKNAD
                     VSLPLRAKIISNKARALSMGERIPESIELMSSALLEAQREGRERDTNILRINLADALI
                     EVERFDEVPELIDTAIAYFEEHKLWDMAQSGYLCKARYFESRQDYQSAFDSMEQLYQV
                     SRRHFQENFSSQSRRYRQRIEDLRNEYLLLKNQCASLDSLGTRNSGQSLIGEHPLIKM
                     AISNSLQAAKYPFVNVHIYGESGTGKEIIARLIHANSQSSKAMIAINAS"
BASE COUNT         2065 a         2497 c         2688 g         2083 t
ORIGIN      
        1 ctgaagccag tgataccggc agcagtttta accagcacgc agaccacctt gcccgtcttg
       61 gagttcaaag cccctggata atatacgcgt ttatctcttt gtttgacggg gctttggagt
      121 tcaagcagga cacggaggag tgattccccc ttcgatgcgc tggttgaagt ccaagcggcc
      181 tgcaaactcg ctgccatata tactcttatc ccggccgctt gaactccaac ggggctaggt
      241 tgaaaccgtg tgtttctcta atcgcctgcc gttctatgct ctcaccactt ccggcagcca
      301 ttcgtccact gccgttttga acagcccttt cagttcgtcc caggcgtcgc ggtccagctg
      361 gccggtccat tcgtccatga aagtcttttt gaattcgatg gccaggcaga agaggtgatc
      421 ggaatagtgg gcgttcagat agcgggccag atagccaccg gggaatttga cgtcggcgcg
      481 gcaatccagc ttgcgacctt gccagctttg tccgtccagg cgggcgcgca gcgattccac
      541 agatttgtga tagcgggcgc tcatgttgct gcgtccgatg atgatatcgg gatttgattc
      601 ttgcggatcg ggctgggcat ttggtccacc ccgccgatga ttgtagctgt ggaggtccag
      661 caccacgatg aagggatggt ggctgagcat gcgtttgatg cagaaatcca ggtgggcata
      721 ccactggtcg tattcggagt agagcgatgc aatgatatgg ggcggaagat tttgcctgga
      781 gaccggcagt ccccagcaat cttctggtac cagatacacc gctttgtcgc gcaggcggtt
      841 gaggtccacc tcaaagcggc tgctgcggag gatgatatga ttggcaaaga gggccgctgc
      901 ttcatcggtg taagggtctt cctctctgaa gcggtcggcc tcggggattc cgcaatggtg
      961 gtccatctcc gggccgagtt gatggccggc atggatggca gtggccagga cggggagttt
     1021 gctgtccagg agggtgaaag cgctgtcgat cacaggaccc atccctgcac tctggtcatg
     1081 agcgcgtcca gcatggcgat ataacggctg gcatcctgga ttcccgtgat ggttagctgc
     1141 aaggggttca ggtccactcc ggagatgggg gtggcgagtt cgatggcctc agccagttct
     1201 ttgggcaccg acaggtcttt cagcgcgaag aatcctttca ggacggggtg gattgctgtt
     1261 ctcgagcgct ggatggcgtc cctcacctga gcggtcttgt agggattttc caacaccgcc
     1321 tggcgggtga gcagccattt gctcctgagc tcgcgctcca tctgcagtct caggtccgct
     1381 ttggcaaaac ggaggccttt caggatgtcc gtgttggcca ggaggttgcg gtatgaggtc
     1441 tgcatattta gaaactccag ggggtaggaa tccagggagt tttccacaaa gatacggttc
     1501 acgatcaggg gttccggaag gcggcgcttg cggatcactc tcaccagcgg ctgcaggaca
     1561 tcaaagaagg agggcggatc atcctcaaag atcaggatgg cgtgttcgac gccgtcgtag
     1621 cctttgtaga tggccacctg caggaggttt tcccgcaggt cccgaaggta actcatcagg
     1681 tcatcgtgtc ggggggtatt catggttttt cctcatattt ttccagatag gtccgggcgc
     1741 tcagcctggc cagatcacga acgcggccaa tgtaggcggc gcgctccgtg acgctgatca
     1801 caccccgggc gtcgaggagg ttaaagtagt gggagcagcg cagcaattgg tcgtaggctg
     1861 gatagaccag tcctttgtcc agcagcgcgt tgcattcttt ttcatgatcc tggaagagac
     1921 gaaagagcag ggtggtatct gcggagttga agttgtaatc agaatactcc tgctcgcggg
     1981 tgaaatagag atcggcgtat ttgacgctat cgctgaacat tagctctcta tagtcgtcca
     2041 cgtcctgaat atacatggcg aggcgctcca ggccataggt gagttccacg ctcacgggga
     2101 agaggtcata gccggccacc tgctggaaat aggtaaactg gctgatctcc atgccgtcca
     2161 gccacacttc ccatcccaat ccccaggccc ccagggtggg ggattcccag tcgtcttcca
     2221 caaagcggat gtcgtgatcg ccaatcgtga ccccgatggc ctcgaggctg cgcaggtatt
     2281 gagtttggat gtcttccggg gtgggtttga tgatcacctg gaactggtaa tagtgctgaa
     2341 agcggttggg gttttcgcca taacgtccgt ccttgggtct gcggcaaggt tggggatagg
     2401 cgatagcggt gggtttggag cccagggagc cgaaaaaggt gccgggatgg aaggtgccgg
     2461 cccccatctc gcaatcgtag ggctgcagga gcgcgcagcc tctttgagcc cagaagtttt
     2521 gcagggtgag gatcatatcc tgaaaggtca ttgcttgtat ttccttttaa gcttgtcgca
     2581 gagggtgagg atgtctttga ggctggacct cagctggccg agttgctctt tgagggctgg
     2641 gtcggagatt gccactggag ggctaagctc ttccagcttg cgatgatagc gggccgcgga
     2701 acgctccgct ttgagcttct gacgggcccc ttcgatggtg aagcgctggg tgtggagcat
     2761 ctcctggatc ttccgcagga gctggacgtt ttcctccgag tatttgcgga tgcgtccttc
     2821 ctccttacgg ggcttcagaa agctgaattc gctttcccaa tagcggatca cgtggggttt
     2881 aacgcccagc aggttgctca cttcaccgat ggtgtaaaag tatttcatcc ggatgcctcc
     2941 ttggatggtg gggccgctgc tttgaacaaa gagagcagaa aaaggccagc ggccaggatc
     3001 aaaaacagca ggggatagga gtggatgtgc ctgatcagtg gcactttatc caccgtgtag
     3061 agcggagcgc cgagattggt gatcttgaaa aggcccgcgg aggcaagttc ccggcccaga
     3121 ggatcgacga tcacgctgat cccggtattg gcgctgcggt agatctgaat gcggttttcc
     3181 accgcccgga acctggtcat cacctgatgc agccagggcc cgtaggacgt gccaaaccag
     3241 gcgtcgttgg tgaggttgac cagaaagtcc atcttggtga tcctgccggt ttggggatct
     3301 ttgggcatgg ccatgcggtg atggatatcc gggaaggcca gctcgtaaca gatggagggg
     3361 ctgtagcggt agccaaagct ctcgtaatag gccagctcct ttccaaactc ccagttggcc
     3421 tggccgaaat tgagcttcca gaggatgggg aactgcttga gccacagcat cctctctccc
     3481 accggtacga ggatgttttt gtggaagacg ggctgctgag agccgtccgg tttgaacagc
     3541 gaggccccgt tgtagtagta aaattcatcc acgtgctctg gaggagcgct gtcaaaatcc
     3601 ggaaagccgg tgaagatctc caggccggtg aggttgatga tctccctcag gtcacgttgg
     3661 cggtccatgt cataacgcag ataggcaggc atggcggcct caggccagat aaagagctgc
     3721 gtgctgtccg cggcggcttt gaggctgagc tcttcaaagc gggccagcat ttggggataa
     3781 tgcttctgct cccatttatc ttcctgtggg atggagggtt gcatcacctg ggcccggagc
     3841 tcccgctttt ggggctggag gctgatgagg caatactggc cgtatccgat ccaggcactc
     3901 atgatcagtg ctatccagat caggttgcgg agcttgcccc gcgaggcctt gtccaccagg
     3961 atgttgatga tcagggccag gagcgccagc agggtcatgc ctcccagatc ggcagcctgg
     4021 atgagctgcg gataggcggc cagggaataa ccgctgttcc accacggaaa gcgtgtctcc
     4081 ccaaagtttt gcagatactc aaagctgagc atgaccgcaa tgaaacccag atgaccccag
     4141 gcgggaaaac ggctccaaat cctctgtatc acatagaaaa acagggccag ccacacggcg
     4201 taaaagccaa cgatgccgat caatcctccc caggtgacct ccgaaatcca gtaaaagacg
     4261 atgcccacat agaccagaga ccagatcaag cccatcagca acaagcgttt aaagctcctg
     4321 ccgccctcct ggagtacaat catcaaggga atccagccga tgaagaccag aaaacccaaa
     4381 tgcagaggca gcctcgccag ggccagcagc agcacgctcg caaaggtgag gatcaggtct
     4441 ttatgcttca cagtggctcc ctatggagcc gcaaacgtcc tcgtttgcgt atagaaaaag
     4501 cctcattgtt ccggcctccg atgcatgatg cgatacaggc caatcccgtt ctcatacacc
     4561 accgcgatgc tttcacgata gacggaagct acacccgatc cagccagcag cggcatcagc
     4621 gaggcctcct caactactgt tccgcgaggc agttcagcca gcacccagtt tccaaaggcg
     4681 tcctgcagcc attgaccttg gcggctttcc ttgaactggc ccaggatgta atagaactga
     4741 gtttcgtccg cggattggct gtaaaccgag agcagatcgc ggttgcaact cagggtggag
     4801 ggggatttaa gctgaaactt gccaaagcgg tagagctcgc tgccgtccag catcgagatg
     4861 cagatcagtt cctgcggagc ggcgtcgtag ataaagaggt cgcggtccgg gctcatgacc
     4921 agcagttcag gctgactgag gctggagagg tccactctgg ccagcagatt gccgtccgga
     4981 gagaatctgc gcagctcttt ggccgccatg tcgagggcca gcaggctgcc gtcgttgtcc
     5041 agcgcgatat ccgtgagcct ctggaaatta taagcctggg tgcccaagcc gccgaaactg
     5101 ttgctctttt tgccttcccg gaagacgtgg atctgctgct ctgccttcag aagcgcaaag
     5161 acactgcccg aaacgggatc ggccacggct ttgtcatagg cgcgttcgct ttggataaac
     5221 tcgctaaagg tgaccgcctc aatctcacgt ttggggttga aacgggctga ggcgcaggag
     5281 cttagcatca ggaaggccat aacgcccgtc aataaggaaa acagagctga gtgtccctgc
     5341 gggccacggc aaacggggac gtttgccact cctctgggat gcatcttcat cgctctcata
     5401 ttctcatttt tcattgtatc gttacccgtt acgcggtttg cggtatgcgt agctgattct
     5461 ccattttcaa ttctcaattg tcaatcgatc tcactgtctc acatgaatat cacgcagttt
     5521 gccctggatg ctggttttgt tgccaaagcg attgtattcc agggtgtagg cgatatcgac
     5581 gatgctgttt ttcttgagca ggctgaggta gtcgcccagg ttgtagccga tcaggtccag
     5641 gctcatgccg tctttcatca ccttgagctt gatgtggttg cgtcccacgt tgtagggata
     5701 gctggccacg cagacgcccc gggtggcaaa gacgggacgg ttgttgtctg gcccgaaggg
     5761 ggcaaagcgt tcgatccaat ccaggaagaa gtcgttgatg tcatagagtt ccagttcgtg
     5821 gtcgatctgg aggggcggct tgatctgttc ggtccgcagg gtgtcggaaa cgtagcgggt
     5881 gagctcgttt tcaaagcggt cgatgtattc ctgatagatg gtgaggccca cggcgtattt
     5941 gtgaccgcca aagctgtgga gattgtgctc ggtgtgtttg agggcgctga agaggtcaaa
     6001 atcccccatg ctgcgtccgg aaccgcttcc gaagccttcc ttgaatgaga tcatgatcac
     6061 gggacggtag tatttttcca ccagtttgga ggccacgatc ccgatcacgc caggatgcca
     6121 gtcgtctgaa gagatcacca tgcagggggt gttggcgagg tccttgtatt tcttttctat
     6181 gatctcgcag gcttcatgga aggtacgctg gtcaagctgt tgtctcagag agttttggcg
     6241 ctcgatgacc tcggccagtt cgtcactctg ggcttcctcg caggagatca gcagttctac
     6301 tgccagagcg gctgagccca tccttccggc tgcgttgatg cggggcgcga tgccaaagac
     6361 gatatcgttg gtatccaagg tcttatggtt cattcccgag atcgacacca aggcgttgag
     6421 gcccaggttt ttcttgtcga taaggtgctg gagaccgatg gacgcgaaga cgcgattctc
     6481 tccggtgagg ggaacgatgt ccgcaatggt gcccaccgcc acgaggtcca tatacttgag
     6541 ggtagtttcc acgttttggt agcccatgcg ctgatagatg gccatcagca gcttgaaggc
     6601 cactcccact ccggccagat ggggataggg atagcgggtg ccgggcagct tgggattgat
     6661 gatggcgagg gctttgggca gctcttcctt ggggttgtgg tgatcggtga tgatgatttc
     6721 catgcccatg gcgttgatag cggcgatttc ctcgagggca ttgactccgc aatccacgct
     6781 gataatgagg ctgctgccgc tttctttgag ctggtccagc gaacccagcg agaggccata
     6841 gccatcgatc atgcggtggg ggatatagaa atcgatctca gcccccagct ttcgcaggcc
     6901 caacaagagc agcgccgtgg ccgtggtgcc atccacgtca taatcaccgt aaatggtaat
     6961 cttttcgtgc ctttcgatgg cctgaacgat ccgggtgacg gccttgtcca tatcctcaaa
     7021 gaggaaggga tcgtgctgat gatagaggct gggagtaaag aagtcccgaa tctcttgctc
     7081 cgttttaagt ccctgccgga aaagcagttc agagaccatg ggagggcttt ttagcgagtc
     7141 gctcagctca cttacgagct gcttttcttc cgcggatagg cctggtggtg tgatccattc
     7201 cttctccatt tttctacctt tattttttta gtttttatcg ggtccgcagg gaaggcactc
     7261 cacagtcaaa gcaggcgcaa aaacgtaact caacacttat caggcatctg tcacgacctt
     7321 cccagaattt acaagatatc cggagggcta aatagtgtaa agagtttttt ttagtgcgga
     7381 gaatcagggc cgcgattcct caaaagggag ggatcggaca ggctgccgga ctaaggtccc
     7441 ggacctgcag ccataaacac atgcataaca ctatgtacgg caattattaa gcaccatttc
     7501 ttgccgtatt tctcccttga gatcgcggtc tccttcccga ttggcattac ggtatcatta
     7561 cggactcatt acggacttag tccgtaatga gtccgtaata ggatgcagga gaaaccaggc
     7621 gagaggctgc tcacatcaat ggttttatcc tggttttatc cactttcaac caccctccaa
     7681 cccgctcaac ccgcttcaac gctgctacgc tgccgcatcc tgatctcccg atctggaagc
     7741 cccaaaacga ttcccgccaa aatagccttg acaacacagc gctcaccgta aatatcgccc
     7801 gccatagtcc gaattgaaag ctcccgaggt atgagcatga accagcgatc ctgcgaagac
     7861 atctactatc aatatcgctc ccaggcgatc aaccaccccc agacctatct ggagagcttg
     7921 ccgtccctga agctcaaagc ccaggtgcca cccatggcat tggccctgaa cagcgtggaa
     7981 gcctggctcg tccggggtga cttttgcaaa gccaggcagg agttggatgc cctggccgcg
     8041 gagatcaaaa agaccgcgga ccccagtctg aaaagccact ggcagctctt gaggcactcg
     8101 ttgatgtttc agacctccgt cgatacccaa accgctcagg aagagctgga caaggcggcc
     8161 agatatcagg ttatgagcgg cagcagcgct ctggagtgcg aggtgttgtt gcaccgctgc
     8221 ctgcaaaaca tgcagccctg ggagatcacc caaagcgaag aaaccctcca gaaagcgatg
     8281 agcgccgcca gcaaatccgg ccagcaggaa ttggtcctgg aagtctacct cgccttcatt
     8341 cagctctacc tcacgcaaaa cctggtggaa caagccaacc gggaactgct tttgatccac
     8401 gacctgatcc agccggatct ataccccctc aaatatctcc agctatacaa tttaagaggc
     8461 atcatcagca acatgaccag gtcctggaaa gaagcggccg aacacttcaa aagcggaata
     8521 aacctggccg aagagcatgg atacggctat cagcttgccc agctctggat gaatctgggc
     8581 atcagcctgg tgagccagag agacttcaac ggcggcatcg caatgtatga tcaaagcctc
     8641 gccatgctga gtaaaaacgc ggatgtgagc ctgcccctgc gagccaaaat catcagtaac
     8701 aaagcccgcg ccctcagcat gggagagaga ataccggaat cgatcgagct gatgagctct
     8761 gccctccttg aagcccaaag agaaggacgc gaacgggata ccaacatcct caggatcaat
     8821 ctggcggacg cccttatcga agtggaacgc tttgatgaag tgccggagct gatcgacacc
     8881 gccattgcct attttgagga acataagctg tgggacatgg cccagagcgg atatctctgc
     8941 aaagccagat actttgagtc acggcaggat tatcaatccg ccttcgacag catggaacag
     9001 ctctaccagg taagcagaag gcactttcag gaaaacttca gcagccagag cagacgctat
     9061 cggcaacgca tcgaagacct tagaaacgag tatctgctac tcaaaaacca atgcgccagc
     9121 ctggacagcc tgggcaccag gaatagcggt caaagcctga tcggagagca tcccctcatc
     9181 aaaatggcca tctccaactc cctccaggct gccaaatacc ccttcgtcaa cgttcacatc
     9241 tatggcgaat ccggcaccgg caaagaaatc atcgcccgcc tcatccacgc gaacagccag
     9301 agctccaagg ccatgatcgc catcaacgct tcc
//
LOCUS       DAOSFN010000100         9264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6340_length_9264_cov_9.936367, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000100 DAOSFN010000000
VERSION     DAOSFN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6340_length_9264_cov_9.936367"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(670..2052)
                     /locus_tag="PLO35_07055"
     CDS             complement(670..2052)
                     /locus_tag="PLO35_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOH79463.1"
                     /translation="MKHLTILIAFTLLCLGLAASELDGQWNGMLDIYGTQLRIVVHVT
                     ETDSGLVATLDSPDQGAYDIPADSISFKDGEVEIVLGSLKASYKGTLEGTKISGTFRQ
                     AWLELPLEFTREEIAAPSYNRPQTPEEPYPYVSRDVSFVNPESGNKLAGTFTLPSEEG
                     VYPAVVLVSGSGGQDRNEELMNHKPFLVLSDYLTRAGIAVLRYDDRGIGESEGDFLTA
                     TTYDLMSDALSAVNWLREQPQVVKTGIAGHSEGGIIAPMAAMMDPAIDFIVLLAGTGI
                     PGDQLLMRQGELVMRAMGTPEEEITRTLAFNRKVYDLILSTTDETEIRAQLDLLVEAA
                     MADSLTNFAPGYTVEEIQESMASQLISPWMINFIRLDPRVFLREIKTPVLALNGSRDL
                     QVPALENLGGITSALLEAGNDSFKVIEYYGLNHLFQFSQTGSPSEYGSIEETFNAAVM
                     KDIAEWILGR"
     gene            2444..3790
                     /locus_tag="PLO35_07060"
     CDS             2444..3790
                     /locus_tag="PLO35_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH79464.1"
                     /translation="MFKRQIISELQCWAERPYRKSLVLRGARQVGKTTAVRMFGKEFD
                     CFIELNLEQPDDRAIFIDGTNVQSLYNLILLRKSLHPQGKRVLLFIDEIQHSAQALLS
                     LRYFYEKMPELYVIAAGSLLDVYLRTKRLEIPVGRVEYLWMYPCNFEEYLEAAGQGQL
                     LDLMKDTQFPDWAQSFLRERFMDYALVGGMPEAIQVWLDTGKIMEVRRVLDGILQSYR
                     DDAVKYAISTDQASVLRHLIETAPTQVTRLISFEHFGASDFRAQAVKNAFTLLEQASL
                     VTLIHPFTSAIMPALPRLGKRPKLLFLDIGFVNAQAGIHDQYFTATGLHSIYKGVAME
                     HLVGQQLLSMASAHGFKLGTWVRNARSSSAEVDFVIIWRGRMIPIEVKAGKTGTLKSL
                     LLFMEECDHDLAVRVYDGQTKWETLTTPSGKQFRLLNLNLGLNTQLISYLEPAIEV"
     gene            complement(3847..>5202)
                     /locus_tag="PLO35_07065"
     CDS             complement(3847..>5202)
                     /locus_tag="PLO35_07065"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOH79465.1"
                     /translation="NNPENWENGMGDWYASKSIWQVGAPTSGPGSGYQSTNCAATILN
                     GNYPARSRSRLTSPSYHIPAGATNLALRFRHWYNVAGGDTLYVQIKESSSTVWQNVSG
                     GIYTGNSGGVWATLTYVPLPDIAGQNINLGFYLATNGGSAPGWYIDNIVIQYTVSNPA
                     PVAIAPTTVTQSGFRANWNVVSGALSYRLDVSTSSGFSSFVPGYQDLNLSTNYCDVSG
                     VNPGCSYYYRIRAVHAAGLSDNSNLISVLTLPPNPVAANSSNLSAISFQANWAAATSA
                     TSYRLDVATDNGFAAFVSGYQDLTLTDTQKLVSGLMPDTWYYYRVRAVNASGVSGNSN
                     MISVHTPEAPPKAPQNLLISTSGSNILLSWDPVTQSIVNTPMTPDYYLIYQSTNPHSG
                     FGFAGVAPSNQYTHLMALNFSSRLFYQVHAYKSSGRVQSRIGDLKPGMSHSEVLSILE
                     E"
     assembly_gap    5203..5302
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5303..5508)
                     /locus_tag="PLO35_07070"
     CDS             complement(<5303..5508)
                     /locus_tag="PLO35_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79466.1"
                     /translation="MTRKTIVSLVLLLGLLAFAQAEVVYSEDFEGNWEDDWYADFGTW
                     EVGTPTSGPGAAYGGSNCLATILAG"
     gene            6181..7560
                     /locus_tag="PLO35_07075"
     CDS             6181..7560
                     /locus_tag="PLO35_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH79467.1"
                     /translation="MKKMPLTPLLPSGAGYVELKQQENRSQNLAFLNGTMVSNNRSSS
                     GGIMARTFTNGVWGLASSVDQSPASMEKVIKKAISNASLLSQKARKGPMALPASSARG
                     DYLFFTQKPELSTAAKIDFLKALDDYAITTYPELSTRQIALYQDEMEKHILTTHGADM
                     HTMTPRSILIVYLMITHNGEALSHYDVFGGFGQFEDNFSDPSAYFSEIDRVYKQLTDK
                     KDAVFARGGTHECVLDADLAGILSHEAIGHTTEADFVINGAIAGDYLDQPVASPIVTL
                     IDVAHTWDGKTCPVPVYIDDEGCEAEDCVIIENGILKRYMHNKESAMLLGHSLTGNAR
                     GYTYSDEPLIRMRNTIIVPGQDNLADMIASIEDGYYLVSTNNGQADATSEFMFGVTLG
                     YEIKNGSLGKAIKETSISGVAFDLLKTITMISGEMKWSCGGMCGKKQPMPVGMGGPAI
                     KCKVNIGGK"
     gene            7560..8852
                     /locus_tag="PLO35_07080"
     CDS             7560..8852
                     /locus_tag="PLO35_07080"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOH79468.1"
                     /translation="MDTRQIMSQALQALLDKGADKVALWLTEKTKEEFNIVYKELNLL
                     RSVESQNFNLIVIKDQKQATTTLNQFDPESVAKAVEEVMANVLSSNADPAFDISPYQE
                     PQTFTDGPMEMDADLIVKRLEEFNAAMRKDYPSVYYDAILSHTRARYQFLNSNGVDFC
                     QNWGYYGFTTMFTAKDGKQMSSFNYTSFETSELDNPLLDYNFTAELMRQITQQTLTQP
                     IPQNFTGEVLLCPFVTGELLDSLISQQFGSSGLLTNSSRFPDHVGQRVLDARLSVYNK
                     PSDPRLAIKDMVTSDGYVAQEGAIIENGVLLHYPIGLFTANKVGKARTFGTADSIVVE
                     AGPTPLAEMIKSIREGVLCMRASFGNPNPNGDLSAVLKNSYYIKDGAIQFPISESMMS
                     INLIDIFNNIVDISRETFNTGSSIYPYIQVKGAAISRK"
BASE COUNT         2146 a         2468 c         2453 g         2097 t
ORIGIN      
        1 cgcataacgc gtaacgggta acgcaaaaca ctgagaaatg agaaaatgag gggttatttg
       61 tagcgtcggt ttccaaaccg gcccgtgcag cgcgtaacct caattctcaa ttctctgtcc
      121 ccctttcgcc acccgtgctt atcgcacgct tatcgcacgc ttatcccgtg cttcggtatg
      181 ggtggagggt gagagacgcg cgagtgaata atcagattgt gaacggggac ctggcctcag
      241 tgaggaggac ggcattttga gcccgcaaga ggtgatattt ctgctggaat gtgcgtgaca
      301 cacatagcct ggacgtagca ccatgcagtg ttcagcaggc tgtggcgata tttcagctgg
      361 aatgtgcgtg acacacattg gtctgagtcc cgtcaggagt agtgttacag atcgttgatt
      421 cacatcggat gccattgtcc tgcagtgatc aaagcatata tcatgcctgc tctgagcccg
      481 gagggcgacc caatgttata gccccccgtg cagcgtagcg gaacgggggg acctgacaga
      541 taattcagtt gagctccccg ggagcggaat accgcctgga accgcgattc atctgcaaac
      601 ccagatgttc tattcagttc ccctcgaacg cagcagctcc tcaagcagtc tcatcagctc
      661 gcagagctct cacctcccca atatccactc cgcgatgtct ttcatcacgg ctgcgttgaa
      721 ggtttcttcg atgctgccgt actcgctggg actgccggtc tggctgaact ggaagaggtg
      781 gttgaggcca tagtattcta tcactttgaa ggagtcgttg ccggcttcga ggagagcgct
      841 ggtgatgccg ccaaggtttt ccagggctgg cacttgcagg tcccggctgc cgttcagggc
      901 caggacggga gtcttgattt cgcgcaaaaa gaccctgggg tccagcctga taaagttgat
      961 catccagggg ctgatcagct gggaggccat cgattcctgg atttcctcca ccgtgtagcc
     1021 gggcgcgaag tttgtgaggc tgtccgccat ggctgcttcc accagaaggt ccagttgagc
     1081 gcggatctcg gtctcgtctg tggtgctcag gatgaggtca tagaccttgc ggttgaaggc
     1141 cagggtgcgg gtgatttcct cttcgggagt gcccatggcg cgcatgacca gttccccttg
     1201 tctcatcagc agctggtcac caggaatgcc agttccggcc aggagcacga taaaatcgat
     1261 tgccgggtcc atcatcgcgg ccatgggagc gataatcccg ccttcgctgt gtcccgcgat
     1321 gccggtttta accacctgag gctgttctct gagccagttt accgcgctga gggcgtcact
     1381 catcagatca taagtggtgg cggtgaggaa gtctccttcg gattcaccaa tgccgcgatc
     1441 gtcgtatctg agcaccgcga tcccggcccg ggtgagatag tcggacagca ctaaaaaggg
     1501 cttgtggttc atcagctctt cgttgcggtc ctgtccaccg ctgccggaaa cgaggaccac
     1561 ggcgggatag actccctctt cggaaggcag ggtaaaggtg ccggccagct tgtttccgga
     1621 ttccgggttg acaaagctaa cgtcccggct cacataggga tagggctctt cgggggtctg
     1681 gggacggttg tagctggggg ccgcgatctc ttcccgggtg aattccaaag gcagttccag
     1741 ccaggcctga cggaaagtgc cgctgatttt ggtaccctcc agagtgcctt tgtagctggc
     1801 tttgagggac cccaggacga tctccacttc accgtcctta aagctgatgc tgtcggctgg
     1861 aatgtcataa gctccctgat cgggggaatc cagagtggcc accagtccgc tgtccgtttc
     1921 agtgacgtgg accacgatgc gcagctgggt gccatagata tccagcatgc cgttccactg
     1981 gccgtcgagc tcggaagcgg cgaggccaag gcatagtaag gtaaaggcga taagaattgt
     2041 taagtgtttc acgttgactc ctttggagag attcagatag tgtggagtaa cctatttgca
     2101 gagaggctgt ggttgtcaag aaaaagggga gcgaaagtga gagctctgcg aactggtgag
     2161 gattgtcatc atgtataagc taaatgtccc tcctaagtca caatcatata tagtcttcgc
     2221 gcgttttaga cttacaaaca acaggtcttg atagagaagc atattagcaa aaccaccccc
     2281 aaacaacctt gactttcgat gcacaattcc catagattac cttgactttc gatgagtatt
     2341 acccccaaac aaccttgact ttcgatgaga aggtggctac aattaccttg acataattcg
     2401 ctatcatatt ttgattgaat caattgaagc ataggagcca gacatgttca aacgtcagat
     2461 catttcagaa ctgcagtgct gggctgagag gccatatcgg aagtctttgg tgctgcgggg
     2521 tgcccgtcag gtgggtaaga ctacagcggt gcgcatgttt ggcaaagaat tcgactgttt
     2581 catcgagctc aatctggaac aaccggatga tcgggctata tttatcgatg gcacgaacgt
     2641 tcagtcgctg tataatctca tcctgctcag gaagagcctc catcctcagg gaaaacgcgt
     2701 gctgttgttc attgatgaga ttcagcattc cgcacaggct ttgctgagcc tcaggtattt
     2761 ctatgagaag atgccggagc tgtatgtgat tgccgcaggc tcactgctgg atgtctatct
     2821 gaggactaag cgcctcgaga ttcctgtcgg cagggtggag tatctctgga tgtatccctg
     2881 taactttgaa gaatatctgg aggcggccgg ccagggtcaa ctgcttgatc tgatgaagga
     2941 tactcaattt ccagattggg cccaatcatt cctgagagag cgttttatgg attacgcgct
     3001 ggtgggtgga atgccggaag cgattcaggt gtggctggat accggtaaga tcatggaagt
     3061 ccgcagggtc ctggacggta ttctgcagtc ctatcgtgat gatgccgtca agtatgccat
     3121 cagcacagat caggccagcg ttttgcggca tctgatcgag acagcgccca ctcaggtgac
     3181 caggctgatc agcttcgaac atttcggagc ttcggatttc agagcccagg ccgtcaagaa
     3241 tgccttcact ctgctggaac aggcctcttt ggtgacgctg atccacccct tcacttcagc
     3301 aattatgccc gctttgccca ggctcggaaa acgtcccaaa ctgctgtttc tggatatcgg
     3361 tttcgtgaat gcccaggccg gtattcacga ccagtatttc actgccacgg gtttacattc
     3421 catttacaag ggagtagcca tggaacatct ggtggggcaa cagcttttat ccatggcctc
     3481 agcccatggc ttcaagcttg gcacctgggt tcgcaatgcc agaagctcca gcgcggaagt
     3541 ggattttgtg atcatctggc gcggccgtat gatcccaata gaagtaaaag ccggaaagac
     3601 cggtaccctg aaatcgttgc tgttgttcat ggaggaatgc gatcacgatc tggccgtgcg
     3661 cgtctatgat ggccagacta aatgggaaac cctcaccact ccatccggaa agcagtttcg
     3721 cctgctgaac ctcaatctgg ggttgaatac ccagctaatc agctatctgg aacccgcgat
     3781 tgaggtttga tttattctcc aggagcggag tcaccattaa ccccgctccc ggagatcgtc
     3841 ataatgtcat tcttccagga tactgagcac ctccgagtga ctcatccctg gcttcagatc
     3901 accgatcctg gattgaacgc gtccactgct cttgtaggca tgcacctgat aaaagagccg
     3961 gctgctgaag ttgagggcca taagatgggt gtattggttg cttggggcca ctcccgcgaa
     4021 cccaaatccg ctgtggggat tggtggattg atagatcagg taataatccg gagtcatggg
     4081 agtattgacg atcgactggg tgacgggatc ccaactgagc aggatgttgc tgccagaagt
     4141 gctgatcagc aggttttgag gggccttggg aggggcttcg ggagtgtgga cggagatcat
     4201 attggagtta ccgctgaccc ctgaagcatt gaccgccctc accctgtaat agtaccaggt
     4261 gtctggcatc aggccgctca ccagcttctg ggtgtccgtg agcgtgaggt cctgatatcc
     4321 actcacaaag gccgcgaagc cattatccgt agccacgtcg agccgatagg aagtggcgct
     4381 ggtagcggcg gcccaattgg cctggaagga tatggcgctc agattggagg agttggcggc
     4441 taccggattg ggaggcaagg ttaatacgga gatcaggttt gagttgtcac taagcccggc
     4501 agcgtgaacc gcccttatcc tgtagtaata tgagcatcca gggtttacgc cgctcacatc
     4561 gcagtagttt gtactcaaat tgaggtcttg atacccgggc acaaagctac tgaacccact
     4621 tgaggttgat acatccaacc gataagacaa agcccccgaa accacgttcc agttggcccg
     4681 gaagccgctc tgcgtgacag ttgtgggtgc aatggccacc ggggccggat tggagacggt
     4741 gtattggatc acgatgttgt cgatgtacca gccgggagcg gaaccgccat tggtggcaag
     4801 atagaatccc agattgatgt tctgtccagc aatatcaggc aatggtacat atgtcagcgt
     4861 ggcccagact ccacctgagt ttccggtgta tattccaccc gatacatttt gccagacagt
     4921 ggaagaactc tctttgatct gaacatacag tgtgtcccca cccgccacat tgtaccagtg
     4981 tctgaatctg agtgccagat tggtggcccc cgcggggatg tggtatgatg ggcttgtgag
     5041 cctggacctg ctcctggccg gatagttgcc gttcagaatg gtggccgcgc agttggtgct
     5101 ttgatatcca gaaccaggac cactggtggg agcgccaacc tgccagatgc tcttgctggc
     5161 ataccagtca cccatgccgt tttcccagtt ttcggggttg ttnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nncccgccag gatagtcgcc agacagtttg atccaccgta
     5341 agccgctccg ggaccgctgg tgggagttcc cacttcccat gtcccaaaat ccgcatacca
     5401 atcgtcttcc caattacctt caaaatcttc actatacacc acttcggcct gggcaaaagc
     5461 cagcaggccc aaaagcaaaa ccaaagacac tatcgtcttg cgcgtcatgt tgcctcctat
     5521 agccacattt tttagtttag aacgcttttc tttattcaca aatcagtctt gcgtgtcaag
     5581 tattaattag gcgtcattat cacgcgctta ggtagaaatg cccttcattt caggattttc
     5641 tgtttttagg cgtttgtgga atcttttcca cgctgactta gcttggtgga tcatttatca
     5701 gtaaatggct acagtggttt gatttacatt ccaagggggg gggattatgt aaccgcttcc
     5761 agaaatccag aaagcacggg caaaacaaga gtgctttgct tgactataag cgagcaatcc
     5821 aaaatcatac gtcaagcttt ggtcctgatt gatcaatttg gcactcaaac actggtttca
     5881 agcccgctga agatattgac caccccagtg cccagacccg catcccccca tcagtcaagc
     5941 atccagggct taaactgtat acttaaaaag gttacttttc agtttatcat ctccccacga
     6001 atcacgcgca tcgctggaac agtggcttca gagcatggat cagggattcc ggctcggcac
     6061 agcggggcac aaaatccaca agtccctaaa attaaatcca aactgcccgc ctttgtcctt
     6121 gacaataatc tcccgttctc ccacaagcga caaatgatag gctaatcaaa aggagttttt
     6181 atgaagaaga tgcctttaac cccgctcctg ccttcaggag caggatatgt ggaactcaaa
     6241 caacaggaaa accgcagcca gaacctcgcc tttctaaacg gcaccatggt gagcaacaac
     6301 cgcagttcca gcggagggat catggcccgc accttcacca atggggtctg gggcctggcc
     6361 agcagcgtgg accaaagccc cgccagcatg gaaaaagtga tcaaaaaggc catcagcaac
     6421 gcctccctgc tctctcagaa agccaggaaa ggtcccatgg ccctgccagc ttcctcagcc
     6481 cggggtgact atctcttttt cacccagaag cccgagctca gcactgccgc caagatcgat
     6541 ttcctcaagg ccctggacga ctacgcgatc accacatacc cggagctcag cacccgtcag
     6601 atcgcgctct atcaggatga aatggaaaaa cacatcctca ccacccacgg ggccgacatg
     6661 cacaccatga cccccaggtc catcctgatc gtctatctga tgatcaccca caacggtgag
     6721 gccttgtctc actacgacgt gtttggcggc tttgggcagt ttgaggacaa cttcagcgat
     6781 cccagcgcct acttctctga aatcgaccgg gtttacaagc aactgacgga taaaaaagac
     6841 gccgtctttg ccagaggcgg tacccacgaa tgcgtcctgg acgccgatct ggccgggatc
     6901 ctttcccacg aggccatcgg tcacaccacc gaagccgatt ttgtgatcaa cggtgccatc
     6961 gcaggggact atctggacca gcctgtggcc agtcccatcg tcactctcat cgacgtcgcc
     7021 cacacctggg acggcaagac ctgtcccgtt cccgtctata tcgacgacga aggctgtgag
     7081 gccgaggact gcgtgatcat cgaaaacggt atcctcaagc gctatatgca caataaagag
     7141 agcgccatgc tgctgggcca ttccctcacc ggcaatgccc gtggctatac ctacagcgac
     7201 gaaccgctga tccggatgcg caacacgatc atcgtcccgg gccaggacaa tctggcggat
     7261 atgattgcct ccattgaaga tggctattat ctggtgagca ccaacaatgg ccaggcggac
     7321 gccaccagcg agtttatgtt tggcgtcacc ctcggctacg agatcaaaaa cggcagcctc
     7381 ggcaaggcca tcaaagagac cagcatctcc ggagtggcgt ttgacctgct caagaccatc
     7441 accatgatct ccggcgagat gaaatggagc tgtggcggca tgtgtggcaa aaagcagccc
     7501 atgcccgtcg gaatgggcgg tcccgccatc aaatgcaaag taaacattgg aggtaagtga
     7561 tggatacgcg ccagataatg agccaggccc tgcaagccct tttggacaaa ggcgcggaca
     7621 aggtggcgct gtggctgacg gaaaagacca aagaggaatt taacatcgtc tataaagagc
     7681 tgaacctcct gcgcagcgtt gaaagccaga actttaacct gatcgtgatc aaagaccaga
     7741 aacaggccac caccaccctc aaccagttcg atcccgaatc cgtggccaaa gcggtggaag
     7801 aggtgatggc caacgtgctg tcctccaatg ccgatcccgc cttcgacatc tcaccctatc
     7861 aggagcctca gaccttcacg gacggtccca tggagatgga cgcggacctg atcgtgaagc
     7921 ggctggagga attcaacgcg gcgatgcgaa aggattatcc cagcgtctat tacgatgcca
     7981 ttctgtccca cacccgggcc cgctatcaat tcctcaacag caacggggtg gatttttgcc
     8041 agaattgggg ttactacggc ttcacgacca tgtttacggc caaagatggc aagcagatgt
     8101 cctccttcaa ctacaccagc tttgagacga gcgaactgga taatcccttg ctggactaca
     8161 atttcaccgc tgagttgatg cgccagatca cccagcagac cctcacccaa ccgattccgc
     8221 aaaactttac tggcgaggtg ctgctctgtc ccttcgtgac gggagaattg ctggacagcc
     8281 tgatcagcca gcagtttggc tcttccggtc tgctcaccaa cagcagcaga tttcctgacc
     8341 acgtgggcca gagagtcctc gatgccaggc tgagcgtcta taacaaaccc tccgatccgc
     8401 gcctggccat caaagacatg gtgaccagcg acggctatgt ggcccaggaa ggagccatca
     8461 tagaaaacgg agtgctgctg cactatccca tcggactctt taccgccaac aaagtgggca
     8521 aggccagaac cttcgggact gcggacagca tcgtggtgga agcgggtccc acacccctgg
     8581 ccgagatgat caaatccatc cgggaagggg tgctctgcat gcgcgcttcc tttggcaatc
     8641 ccaatcccaa cggagacctc agcgccgtgc tcaaaaacag ctattacatc aaagacggcg
     8701 ccatccagtt tcccatcagc gaatcgatga tgagcatcaa cctgatcgat atcttcaaca
     8761 acatcgtgga tatctcacgc gagaccttca acaccggctc ctcgatctat ccctatatcc
     8821 aggtgaaagg agcggcgatt tcacggaagt aattgagaat tgagaattga gaattgagaa
     8881 ttgagaattg agaattgaga attgagaatt tagctgcgcg ctagcaggtg gatggcaaac
     8941 gtcctcgttt ggtcgttatg cgttacgcgg gaagcggtat gcgggaagcg cgcctgcctg
     9001 tggattggca aacgtcctcg tttgccgtgg cccgtaggga cactctctct gcctttctct
     9061 ggattaagct ctgcttttct ctgtgtttta tacgtgatgc gttccgcttt atgggcacgt
     9121 gccgttgcca tccaggtctg cctttcatat ctcagaccta tcccagactc ctcccatgct
     9181 gaagcacggg atactgaagg gaaggaaagc cccgttggag ttcaagcggc cacgataaag
     9241 ctatgcatgg cagcgagttt gcgg
//
LOCUS       DAOSFN010000101         9100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6492_length_9100_cov_9.735213, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000101 DAOSFN010000000
VERSION     DAOSFN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9100
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6492_length_9100_cov_9.735213"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..277)
                     /locus_tag="PLO35_07085"
     CDS             complement(20..277)
                     /locus_tag="PLO35_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79469.1"
                     /translation="MEPEKKKNYLPNAIFKAIPLVALVIMGVLDLIDGNIYPGLALIV
                     FGLMFAYEIYVNLVRKREVRNLEQLPFVVVGGVLVILHYVL"
     gene            complement(298..3216)
                     /locus_tag="PLO35_07090"
     CDS             complement(298..3216)
                     /locus_tag="PLO35_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79470.1"
                     /translation="MKHSVIFICMICAWGLLAAQNLPLRQAEVIHTSYSSAVLTDGSV
                     VTVWAEARINDYDLYAQRFSSTGQALWSEPLGLVVRDGIDNSASLAATSDGGFVLVWH
                     TDFSAGGFMLQKFSSSGEALWDQASHTYPLGSASTQGLHILPNPQGGIYLLRSSFQSG
                     YRLRAFSLDASGAHLWAPDGIQLGSQSSDTIYNGSISDGSGGLIVNYNLSGGVGSQLI
                     HLDQSGSVVGPNPMLATNPFGSSSYSIMRLSNGNYALYSYSSSAYTLSWQIMDPGGNL
                     LMSSPLTTNLYSNAGVPKLAPSTDGGFYISYLRSPQIHLLRINASGSPVWQPWAKTFA
                     ANVGRGTLFSLLTDSQNRPLITWRDVTDNTNRSLLITPDGLPAWPQAGIAVNSNSRDF
                     HLPSVLKVGQEYWYFWKDKAGTSQFFRRQILNGQAAAQLPVGGEALSERLDGAVNTIS
                     SQFTLGAHFYTFWSDNRGGKYELYMQKSGLNLNPLWELRGRKFLAGQPGYKYPVEIMT
                     TPQGQIAVLYRKTGDDPNDYLHYLQVLDPDGNPQLPGLGVALMGSYYTGIGAHLSYED
                     GDYYVVWGNGGSYGGTIRGQRIHEGVAMWPDEGGAFGKILLQHDSGFSIEELANRFMM
                     INFDYPVPGDPGNRIFLLPNDQPERIDWPPDGLIYISNYDSGPDWVVDAQVVQDSLYV
                     VLDGWERDLVQKVGYGAELPWGLDGRITHMPADDYDCHDVRFEDDAIYMLYVKNSYSS
                     DTDIYLQKLDYEGVFNWGEDGRLVMEGGEECYDGRLMKFANGSVGAIWSRAYNGYDDT
                     RLEYAVMDSLGNWDIAPQVFEDGYMTQNYPHVAMLGNRAMLSWVDRRIGYDSGYDYPN
                     SLYARLLDLDATHADDPYAPGLAEFHVLMQNYPNPFNPSTTISYKLERPSRAHLAVYN
                     LKGQVVKVLSSGQLQAPGHYQVVWDGSDSSGQTVSSGVYIYRLSCGGRAVAKRMVMMK
                     "
     gene            complement(3421..6381)
                     /locus_tag="PLO35_07095"
     CDS             complement(3421..6381)
                     /locus_tag="PLO35_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79471.1"
                     /translation="MKKNLLAIAVVLLAVTGLLAEAFSIRQAFNLRWEGQYLSLADDQ
                     KLVFWTDTPSGHRDVFAQKIGSQGQALWTGPRSVVDHENDQVLLSVVNASDGNLILLS
                     GELEIDQIWQLSLQKIDPEGQKLWGPSGITIPAPEHDVGKAMLLPNQVGGAFIIFQDW
                     FQPIVWGQSLSSSGQPLWGQIYQELYSLPLDAFLNIKQVISDGSGGFIVCVDREHAGI
                     SATYLTRFSSLGTVFGNNPLVDLDSLPSPLYNIQACPSGDILLYNTEHPVDSEFWQCK
                     IDNGGQIIQAPVFFNLPINNYPQIPPVMKATPDGGSLLCYSFEVGNQQTLYAQKFNPQ
                     LQMQWPYPGLEIGSGSVVGFRNLDFCVTASGAAWITWIEGSADIEQELAKAQYISPDG
                     SLPWSVNGLAVGLSAYYHVRPLSIPLSDRGLFLWFNRLNGMDGLNRQVLTSGGNPILP
                     NGGEALISRRAGSAELNECLALDNHYVTVWDDVRDSQKIYYQVLDTNLQALLPEGGQA
                     LNPDTTNEEFIRAVAKTPWNSVVIVYINRVSSTVFECYLQEINASGGRVYPGLGIPLP
                     GSIDIDPSSLSFDAGSIYLGWIGYDGVSSFQLWGQRISNGLKQWGEAGRVIVDLPANC
                     FASLDAVQAPYYLWRLEDYNQNEVLCLALKVDQDGNPAPGWDAGGLNLITDTGFYNRS
                     LQHSGHLDGDLVAFIKLDSLNSHTLRAQRISASGTRLWTDSGVSISPDTNLGTLYDAV
                     YDDQIGFVYDTPEIGHFQLRFQMLSPAGQLLLPQATLLDPDSSQSYIANLLRFEDNSW
                     LCSWSDIDSEGWASDVYARHFAPDGQLLDQEPILICGARYNQDRVQADVIGNQALIAW
                     TDSRCGILNSDEAIYGIWAEAYVSSYVSSDDPEAPIQPPALLHANYPNPFNPSTTISY
                     TISKPGLTSLSIYNLKGQLVKTLLPETMQGSGRYNLSWDGTDTSGMPSASGVYIYRLR
                     HEGRDRSGRMVLMK"
     gene            6561..>9100
                     /locus_tag="PLO35_07100"
     CDS             6561..>9100
                     /locus_tag="PLO35_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOH79472.1"
                     /translation="MNIHNLDNIFKPKRVAMLGIGMNPKSVGGKILSNLVSGGFKGVV
                     YPVNPDLESVSGIQAYPDLASLPKLPDLAIICSAPQAVPEYVRQCGERGIHGIIIVSA
                     GFKEVGAEGAALEAEVRDLCQRYDGMRIMGPNCLGYISPSQNLNVSFAEGMPRKGNIA
                     FVSQSGALCTAVLDWAIEEKIGFSYFISIGNTMDVDFGDIIDYLGEDESTESIILYIE
                     SIADARRFMTAARSFALSKPIIAYKAGRFPESAEVAASHTGALASADDVYDAAFQRAG
                     IARVYDIGKIFNVVELIGRKKVPAGARLAIVTNAGGPGVMATDVLIEAKGELAKLADN
                     TIAQLNLELPDFWSHGNPVDVLGDARPKRVSKAVGIVMADPGVDAILVILTPQSMTNP
                     SGTAREIATLAATAKKPILAAWIGGKSMKEGIEILSEAGIPAYATPEQAIGAFMTLVA
                     YSRNLKALYETPKDVPVEFAIDRHQFKTEFESILNQDNKILGEELSKQLLETYGVATA
                     RPRSASTPEEAVKLAAEMGYPVVLKVLSPDISHKTDAGGVMLNIGTEAAVREAFDQIV
                     ANARAYNSQARIEGVTVQPMIDTKDGVELILGIKQDPVFGTVLMAGMGGIYAELFRDK
                     ALELPPLNEKLARRMLEGLQIHPLLRGYRGKPAVDMDALVEVLIRLSYLAADYPQITE
                     LDINPLLVSKQGAVALDCRVVVDNSRRDDGHYSHLALRPYPEEYTRVCKLSDGSEVIL
                     RPIKPEDEPLWLDMLGSCSKDTIYERFRYFFNWDSHTAAVRYCFIDYDREIAIVAETM
                     VEGAKKLVGVGRLVADPDHSSVEYAVLVTDAWQNKRLGSILTEFCMEIS"
BASE COUNT         1999 a         2534 c         2591 g         1976 t
ORIGIN      
        1 caacttgcct gatatgtctc tagaggacgt aatggaggat aaccaatacc ccccccacca
       61 cgacaaacgg cagttgttcc aggtttctca cctcgcgttt gcgtaccagg tttacataaa
      121 tctcatacgc gaacataagg ccgaacacga tcagggccag gcccgggtag atattgccgt
      181 ctataaggtc cagaactccc atgatcacga gagctactag ggggatagcc ttgaagatcg
      241 cgttgggcag atagttcttc ttcttttctg gttccatttt tcctcctgaa tttgatgtta
      301 tttcatcatt accatgcgtt tggccacggc acggcctcca cagctcaatc tatagatata
      361 cacgccgctg ctgacagtct ggccggatga atcgctgcca tcccagacca cctgataatg
      421 gccgggtgcc tgtaactgac cggagctcag caccttcacc acttggccct tgaggttgta
      481 aaccgccaga tgagcccgag agggacgctc cagcttatag gaaatggtgg tgctgggatt
      541 gaagggattg gggtagtttt gcattaaaac atgaaactcc gccaggccag gagcataggg
      601 atcgtccgcg tgggtggcgt cgaggtccag aagcctggca tagaggctgt tgggatagtc
      661 gtaaccgctg tcataaccga tgcggcgatc cacccagctc aacatggcgc ggttgccgag
      721 catcgccaca tgaggatagt tttgcgtcat atagccatcc tcaaagacct gaggagcgat
      781 gtcccagttg cccaggctgt ccatcacggc atattccagc ctcgtatcgt catagccgtt
      841 gtaagcgcgg gaccagatcg ctcccacgct gccgttggca aacttcatca agcggccgtc
      901 atagcattct tccccgccct ccatcaccag acggccatcc tcgccccagt tgaagacgcc
      961 ctcgtaatcc agcttttgca gatagatatc cgtatcggat gaatagctgt tcttcacata
     1021 gagcatgtag atcgcgtcgt cctcaaagcg cacgtcatga caatcgtaat catcggcggg
     1081 catgtgagtt atcctgccat ccaggcccca gggcagttcc gcgccatagc caaccttttg
     1141 caccagatca cgttcccagc cgtccagcac cacataaagt gagtcctgca ccacttgggc
     1201 gtccacgacc caatcgggac cgctgtcata gttggaaata tagatcaggc catccggggg
     1261 ccagtcgatg cgctcaggct gatcgttggg cagcaggaag atgcggttgc ccggatcacc
     1321 gggaacggga taatcgaaat tgatcatcat aaagcggttg gccagctctt caatggaaaa
     1381 gccgctgtca tgctgcagca aaatcttgcc aaatgcgcct ccctcatcgg gccacatggc
     1441 tactccttca tgaattctct ggccgcggat agtcccaccg taggaaccgc cattacccca
     1501 aaccacatag tagtctccgt cttcatagct caggtgagcc ccgatgccag tgtaatagct
     1561 gcccatcagg gctactccca ggccgggaag ctgagggttg ccgtcggggt caagcacttg
     1621 caagtaatgc agatagtcgt tggggtcatc gccggtcttc cgatagagaa cggcgatctg
     1681 gccctgcggg gtggtcataa tctccactgg atacttgtaa ccaggctgac cggccaggaa
     1741 tttgcggccg cgcagttccc agagcggatt gaggttgagg ccgctctttt gcatgtaaag
     1801 ctcatacttg ccgccgcggt tgtcggacca gaaagtgtag aaatgcgctc ccagcgtgaa
     1861 ctggctactg atagtattga ccgcgccgtc cagcctttcg gagagcgctt cgccgccaac
     1921 tggaagctgg gccgcggcct ggccgttcag aatctgccgc cggaaaaact gcgaggtgcc
     1981 ggctttgtct ttccaaaaat accaatattc ctggcctacc ttcagcacgc tgggtagatg
     2041 gaaatcccgc gagttggaat tgaccgcgat ccctgcttgg ggccaggctg gcaggccgtc
     2101 cggagtgatc agcaggctgc ggttagtgtt gtccgtcaca tcccgccagg tgatcaaagg
     2161 ccggttctgg ctgtccgtca ggaggctgaa caaggtgccc cggcctacgt tggcggcaaa
     2221 ggtttttgcc cagggctgcc agaccgggga cccgctggcg ttgatcctca gcagatggat
     2281 ttgtgggctc ctcagatagc tgatatagaa gcctccatca gtggaagggg ccagcttggg
     2341 cactcccgcg tttgaataga gattggtggt gaggggagag ctcatcagca ggttgccacc
     2401 cgggtccatg atttgccagg agagggtgta ggcggagctg gaatagctgt aaagcgcgta
     2461 attgccgttg gatagcctca taatggaata gctgctgctc ccgaacggat tggtggccag
     2521 catgggattg gggcctacta cagatccgga ttggtcgaga tggatgagct ggctgcccac
     2581 ccctccagac agattgtagt tcacgatcag gccacccgag ccgtcactta tagagccgtt
     2641 atagatagtg tcgctgctct gagacccaag ctgaatcccg tccggggccc agagatgagc
     2701 tccgctggcg tctaagctga aggctctcaa tcggtagcca gattggaaac tagatctcag
     2761 gagatagatg ccgccctgag gattggggag gatatggagg ccctgagtgc tggcgcttcc
     2821 caaaggataa gtgtggctgg cctggtccca caaagcctct ccgctgctgc tgaacttctg
     2881 cagcataaaa ccgccggcgc tgaaatccgt gtgccagacc agcacaaatc ctccgtcgga
     2941 agtggccgcc agactggcag agttgtctat gccgtcgcga accaccaaac ctagcggctc
     3001 tgaccacaag gcctgcccag tggagctaaa ccgctgggcg tagagatcgt agtcgttgat
     3061 tctggcctcg gcccaaaccg tgaccactga gccgtccgtg agaaccgcgg agctataact
     3121 agtgtggatc acctcagctt gccgcaaagg cagattctgg gccgcaagca agccccatgc
     3181 acagatcatg cagataaaga taacgctgtg tttcaaagcc tgtctccttg tatatgtctc
     3241 tatcaccgaa tatttgtatc actcaatctt gtcaacaaat gtttaagcac atgctttaca
     3301 ctgtctatca cgcttgcact ggttgaagtc cacgcggccc gcaatctcgt tgtcatgcat
     3361 cgctttatcg tgaccgcttg aactccagcg gggttattca cgatctgtcg gattgagact
     3421 tcacttcatc agcaccatgc gtccggatct atccctgccc tcatgacgta gacgataaat
     3481 atatactccg cttgcgcttg gcatcccgct ggtgtctgtg ccatcccagc tcaggttata
     3541 gcggccggag ccctgcatgg tctctggcag cagggtcttt accagctggc ccttgaggtt
     3601 gtagatgctc aggctggtaa gtcccggttt tgaaatggtg taactgatcg tggtgctggg
     3661 gttgaagggg ttgggatagt tggcatgcaa cagggctgga ggttggatgg gcgcttcggg
     3721 atcgtccgag ctcacatagc tgctcacgta agcctcggcc cagattccat aaatggcctc
     3781 atcgctgttc aggatgccgc agcggctgtc cgtccaggct atgagggcct gattgccgat
     3841 cacgtctgcc tggacgcggt cctgattgta tctggccccg cagatcagga tgggttcctg
     3901 gtccaggagt tgtccatcgg gagcgaaatg acgggcatag acgtccgaag cccatccctc
     3961 gctgtcgatg tcgctccagg agcaaagcca ggagttatcc tcaaagcgaa gcaggttggc
     4021 gatgtaagac tggctggaat cgggatccag cagcgtggcc tgagggagca ggagctgccc
     4081 ggccgggctg agcatctgaa acctgagctg aaagtgccca atctccgggg tgtcatacac
     4141 aaagccaatc tggtcgtcat agacggcgtc gtagagggtg cccagattgg tgtcggggga
     4201 aatgctgacg ccgctgtcgg tccagagcct ggtgcctgaa gcgctgatcc tttgtgccct
     4261 cagggtgtgg gaattgaggc tgtcgagctt gataaaggcc accaaatctc cgtcgaggtg
     4321 accgctatgt tgcagagagc ggttgtagaa acccgtatcg gtgatgaggt tcaggcctcc
     4381 cgcgtcccat ccgggagcgg gattgccgtc ctggtccacc ttgagggcca ggcacagcac
     4441 ctcgttctgg ttgtagtcct ccagacgcca cagatagtag ggtgcctgca ccgcgtccag
     4501 ggaggcaaag caattggccg gaaggtccac gatcacgcgt ccggcctcac cccactgctt
     4561 gaggccgttg ctgatcctct ggccccagag ctggaagctg cttacgccgt cgtatccaat
     4621 ccagccgagg tatatgctgc cggcatcaaa gctcagggaa ctggggtcga tgtctatgct
     4681 gccggggagc ggaatgccca gaccgggata gactctgccg ccactggcat tgatttcctg
     4741 cagataacat tcaaagacgg ttgagctgac gcgatttata tagacgataa ccacgctgtt
     4801 ccagggagtt ttggcaacgg cccggatgaa ttcctcgttg gtcgtgtctg gattgagcgc
     4861 ctggcctcct tccggcaaaa gggcctgcag attggtgtcc agcacctggt aatagatttt
     4921 ttgagagtcg cgaacgtcat cccagacagt cacatagtga ttatccaggg ccaggcattc
     4981 attcaactct gcgctacccg cccgtctgga gatcagggct tctcctccgt ttggcaaaat
     5041 gggattgcca ccgctggtga ggacctggcg gttcagaccg tccatgccgt tcaatcggtt
     5101 gaaccacaag aacaagccgc gatcggaaag gggaatggac agagggcgga catgatagta
     5161 ggcagacaac cccaccgcga ggccattcac gctccagggc agtgatccgt ccgggctgat
     5221 gtattgcgct ttggcaagtt cctgttcaat atcggcggag ccttcaatcc aggtgatcca
     5281 ggccgctccg gatgctgtga cgcaaaaatc caggttcctg aatccaacca cgctgccgga
     5341 gcctatctcc aagcctggat atggccactg catctgaagc tggggattga acttctgggc
     5401 gtagagcgtc tgctgatttc ccacttcaaa tgaatagcag agcagcgatc ctccatcagg
     5461 agtggctttc atgacgggcg ggatctgagg ataattgttg atgggcaggt taaagaagac
     5521 tggtgcctgg atgatctgtc ccccgttgtc gatcttgcac tgccagaact cactgtctac
     5581 tgggtgttca gtattgtaga gtaagatatc tcccgaaggg cacgcctgga tattgtaaag
     5641 cggggagggc aggctgtcca ggtccaccag gggattgttg ccaaacaccg tccccaaaga
     5701 tgaaaagcgg gttaagtaag tagctgagat gccagcatgt tcacgatcaa cacagacgat
     5761 aaagccaccg ctgccatcgg atatgacctg tttgatgttg aggaaagcgt ccagtgggag
     5821 gctgtaaagc tcctgataaa tctggcccca gagaggctga ccgctggagc tgaggctctg
     5881 gccccagacg atcggttgaa accaatcctg aaagataata aaggcaccgc ccacctggtt
     5941 gggcaacagc atggccttgc cgacgtcatg ttccggagcc ggaatcgtga tcccgctggg
     6001 accccagagc ttctggcctt cgggatcgat cttctgcagg ctcagctgcc agatctgatc
     6061 gatctcaagc tcaccgctga ggaggatgag gttgccatcc gaggcattga ccacagacag
     6121 cagcacctgg tcgttttcat ggtccaccac agaccgtggc cctgtccaca gggcctgtcc
     6181 ctggctgccg atcttttggg caaacacgtc cctgtgtcca gagggggtat ctgtccaaaa
     6241 gaccagtttc tggtcatcgg ccaggctgag atactggcct tcccatctca ggttaaaggc
     6301 ctgacggatg ctgaatgctt ccgccagcaa acctgttacg gcgagtaata ccacggctat
     6361 cgctaagagg ttctttttca tgatgttctc cttgttcgat gcatgcaggg taagtgagca
     6421 atgtttgttc cacacacgcc gaaaaaacta tatttctctt ctaagcctca atatcttgag
     6481 tatagccggg cgggaaaatg cttgacctga aagcgcgcgc agataaactg cccttaataa
     6541 gtatcttcag aggtgatgac gtgaatatcc acaacctgga caacatcttc aagcccaagc
     6601 gcgtagccat gctgggcatc ggtatgaacc ccaaaagcgt gggaggcaag atcctcagca
     6661 acctggtgag cggtggtttc aagggagtag tgtatcccgt gaatcccgat ttggaatccg
     6721 tttcagggat tcaggcctat cccgatctgg cctccttgcc caaactgccc gatctggcta
     6781 tcatctgttc cgctcctcag gccgtgccgg aatatgtgcg ccagtgtgga gagaggggca
     6841 tccacggcat catcatcgta tcggccggtt tcaaagaagt gggggcagaa ggtgccgctc
     6901 tggaagctga ggtgagagat ctctgccagc gctatgacgg catgcggatc atggggccca
     6961 actgcctggg ctatatctct cccagccaaa atcttaacgt gagtttcgcg gaaggtatgc
     7021 cccgcaaggg caacatcgct ttcgtgtctc aatccggagc gctctgcacg gccgtgttgg
     7081 actgggcgat cgaggaaaag atcggcttct cctacttcat ctcgatcggc aacaccatgg
     7141 acgtggattt tggcgacatt atcgactacc tgggtgaaga cgagagcacc gaatccatca
     7201 tcctctatat agaatcgatt gccgatgccc gcaggtttat gaccgccgcg aggtctttcg
     7261 cgctcagcaa gcccatcatc gcttacaagg caggccgttt tccggaatcg gcagaagtgg
     7321 cggcctctca caccggagct ctggcttccg cggacgacgt ctatgacgcg gccttccaac
     7381 gagccggcat tgcaagggtt tatgacatcg gcaagatctt caacgtggtg gaactgatcg
     7441 gccgcaaaaa ggtgccagcg ggagctcgtc tggccattgt taccaatgcc ggaggccctg
     7501 gagtgatggc cacggacgtc ctgatcgagg ccaagggcga gctggccaaa ctggcagata
     7561 acaccatcgc ccagctcaat ctggaactgc cggacttctg gtcgcacggc aatcccgtgg
     7621 acgttttggg agacgcgcgt cccaaacggg tctccaaggc cgtcggcatt gtgatggcag
     7681 atcccggagt ggatgccatc ctggtgatcc tgacaccgca gtcgatgacc aatcccagcg
     7741 gcacggcccg cgaaatcgcc accctggccg ccacagccaa aaagcccatc ctggccgcct
     7801 ggataggtgg taaaagcatg aaggaaggca tcgaaatcct ctccgaagcg ggcattccgg
     7861 cctacgcgac tcctgaacag gcaatcggcg cgtttatgac cctggtggcc tattcccgta
     7921 acctcaaagc cctctatgag acccccaaag acgtgccggt ggagtttgcc atcgaccgtc
     7981 atcagttcaa gactgagttt gagtctattc tcaatcagga caacaagatc ctgggcgaag
     8041 agctctccaa acagctcctg gaaacctatg gtgtggccac ggccagaccc aggtccgcat
     8101 ccactcctga ggaagcagtc aagcttgccg ctgaaatggg ctatccggta gtcctgaagg
     8161 tgttatcccc cgatatttct cataagactg atgccggggg agtgatgctg aacatcggca
     8221 ctgaagccgc tgtgcgggaa gcatttgacc agatcgtcgc caacgcccgg gcctacaatt
     8281 cccaggccag gatcgagggt gtgaccgtac aacccatgat cgacaccaaa gacggcgtgg
     8341 agctgatcct gggcatcaag caggaccccg tgtttgggac ggttttgatg gctggcatgg
     8401 gtggcatcta tgccgagctc ttcagggaca aggctttgga gctgcctccc ctcaatgaaa
     8461 agctggcccg caggatgctg gaaggcctgc agattcatcc tctcctcaga ggttaccgcg
     8521 gcaagccagc cgtcgacatg gacgccctgg tggaagtgct gatccggctt tcctatctgg
     8581 ccgccgatta tcctcagatc acggaactgg acatcaatcc cctcctggta tccaagcagg
     8641 gagctgtggc cctggactgc agggtcgtgg tggataacag ccgccgggac gacggtcatt
     8701 actcgcatct ggccctcagg ccatatcccg aggaatacac ccgcgtctgc aagctcagcg
     8761 acggcagcga agtgatcctg aggcccatca aacctgaaga cgaacccctc tggctggata
     8821 tgctgggaag ttgttccaaa gataccattt acgagcgttt ccgctatttc ttcaactggg
     8881 attcccacac cgccgcggtg cgttactgct tcatcgatta cgaccgcgaa attgccatcg
     8941 tggcggaaac catggtggaa ggcgccaaaa agctggtggg agtgggacgc ctggtggccg
     9001 atccggacca ctccagcgtg gaatatgccg tgctggtgac ggacgcctgg cagaacaagc
     9061 gtttgggctc cattctcacg gagttttgca tggaaatctc
//
LOCUS       DAOSFN010000102         8991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6599_length_8991_cov_10.091876, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000102 DAOSFN010000000
VERSION     DAOSFN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6599_length_8991_cov_10.091876"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4139)
                     /locus_tag="PLO35_07105"
     CDS             complement(<1..4139)
                     /locus_tag="PLO35_07105"
                     /inference="COORDINATES: protein motif:HMM:NF019295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOH79473.1"
                     /translation="MSKYILTTLLVLLISFGWLTAQTTVTIGTGTNLQRQPFGAWYGY
                     ERSAAIYRSSDINALGTITHLGWYVGTAQSASVPTKIYLKTTSTNTLTSSTWANIISG
                     ATEVYNASRTFTPSGWLTIDITDFNYSANNLLVLCETNYGGSGTVSYPTFRYSNSNNS
                     HQYWAQDGSVPTGNGTVDSYCPNIQITIIASTVTDPTSFTATPVSASQIDLGWTKNAA
                     NNDVMVAWNSTNSFGTPVNGSSYNLGSTIGGSGTVLYTGSLTAFNHSSLSSNTSYYYR
                     IWSYDAVYDYSSGVSTNATTYVGSYPWNENFDSVTAPTLPSGWTVLNVNADAYTWDNQ
                     TTTPNSSPNAMYINYNASQASNDWLVSPGLYLEAGTDYIAGFAYRARSATYPESMRLN
                     IAASASAVALSAGTQLYLDDGFVHTVYYDAEQYFTVPSTGIYYLGWHAFSAANQWGIY
                     LDDVSVQVAVPIPGVSETSIAFGVTDLEDGSPAREFRFYNLGGGTLNLQGMEILGGDA
                     DQFELVDANSYPIGLARDEYVSVTLYFRPDLVGAKTSDLNIQDDLGKNLYSIPLSGHG
                     AIEKFRDGFESATDFSLTLPGWTQYDGDGSATYSISGVSFLNQGYTGSYIAFNPSATT
                     PPLSALWNAYDGARYAACLAATTPANNDWLISPAISFGQNPVISFYARSITNAYGLER
                     FKVLWSTTGNSYSDFTNYLAGSAGTYVEAPTSWTNYVYTLPPACENTNVYIAIQCVSN
                     KAFSFQVDNFVAGDYGQPQFHISPSSYVFEDFFINYTREQLFTVTNNGGGSMQVVADG
                     ITISGDAYFKLKDLPALPITLGAGQSFSFTVYYNSPRAGSHSATLSVTDNLSKAIHTV
                     AISGSTTDNTITQKPYMEGFEPSYEGGTEFQVQGWVRRDNNGDGYPWLLVNDPSMAKS
                     GNWLAVSQSWAPDAKASSLRLEALEADATSSMQTKAAKTARDRLVLNPDNWLISPPIT
                     ISAGDSLNYWIGAYSDTWYAEHYALLISTGDPDPSQFTTTLTEETLTTDAWQYRAFNL
                     DAWDGQTVYFAFRHYNCTKQLALRIDDVKIKADNTEIYQDYVGDPVQGDNYFKITMQQ
                     NIQDEMNLIPLTVEIEGWLPEASNLLVTGSVGYAQPQIYIENAGLTVLVNGVDMAGAT
                     VRITHNLGYLPLNLAYRSLSGNFSFVTPAQSSLWTTTQVEFLVPAAKAGEGLEIVFPD
                     TDDSTLPVELSSFTVSITGNFQIQLLWVTQSETNLLGYQIYRGESDDFARAIRLEAFI
                     MATNTSQQQLYAFTDTELCQSGKYYYWLEHLEMDGSSHLHGPVLAVYTLPEGSTPGVP
                     VIPGISSIYPNPFNPDTTIRFGLKQQADYRLSIYNLRGQLVRELDSGSLPAGYHKVMW
                     NG"
     gene            4439..4825
                     /locus_tag="PLO35_07110"
     CDS             4439..4825
                     /locus_tag="PLO35_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOH79474.1"
                     /translation="MITYTDAARAWMDGLDDKDRQRLGPVLELFDAHTDQASADYYHP
                     HILVLKLKGQPALALSPRKQYLSFYAWSMQTMGILKAALPKLGKANPGVGCLRFRKAS
                     DLNMDTLAEVFKQMNAVEHEAGFGFQ"
     gene            5110..5403
                     /locus_tag="PLO35_07115"
     CDS             5110..5403
                     /locus_tag="PLO35_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79475.1"
                     /translation="MLENNYNQEPQRRFQYPGVMEAPVVGVFDWVVIMILLALPIVNI
                     IMLIVWATDQQGNPNRANFAKATLIIIAVQIVLIAVLVGTMMGTVMAFADKFA"
     gene            5716..7158
                     /locus_tag="PLO35_07120"
     CDS             5716..7158
                     /locus_tag="PLO35_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH79476.1"
                     /translation="MRYLDLAIGEALNLGAEYADIRIQRTTDEVVLLRNLSLKNTSRD
                     VLHGYGIRVFKDGAWGFAHHHVFTEEAVLATTRRAYEIAALSASVNQNHKLRLAPERG
                     YLASYKTPLKIDPFEVPLGQKVELMMEVNRVMLGYEGIRQAMFYLISHKDEKLFASTL
                     GTRLELNTQWIDPMVIASAVHAGDSQSRTFDEGGRATGWEWVTELNLQEKARQIAEEA
                     LIKVKADTLGPEERRSLILDPNHLGLTMHESVGHPTELDRVLGWEADYAGISFATPEK
                     LKNYRYGSEIVNFVGDNTLDQGLATLGFDDDGVPGQKWHIIKDGILNEYGSTRDTALE
                     IGLEHSRGCNRATYYFDQPINRIPNLYMLPGTQPLTPSQLIADTEDGVYIQGRGSFSI
                     DQHRVNFQFGGDFFWEIKNGKLARPLKKVIYKSCNPEFWNSCDAICDERWWRPFGVVN
                     CGKGQPSQTARMTHGSAPARFRNIRVGGSQ"
     gene            7155..8435
                     /locus_tag="PLO35_07125"
     CDS             7155..8435
                     /locus_tag="PLO35_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOH79477.1"
                     /translation="MNSSFERIAQYIKTHCQADDYSLRIFHADSHETRFAQNRVTQHL
                     AGPKLRVNLNVAFDNKTGSASVNQDDPEALARLIQSAEAIARLNQPDPEYIFSAPQAQ
                     LPEVQNADPATRDLDPARMVQIVQSMIMKAKAMDATVSGMTEKHFSEWALITKGGFEG
                     YDASTEFGHSMTLKKEEVETKVSYEAKSFAGFEPDAEFETLKAQAASLASKQVMEPEK
                     IAVILRPGALMELLWFMGWMMNRRQSDEGFTPFTDQMGKLFFGENFNLYSTLKRTDIK
                     ASPFNSEGIPSREIAWVEKGVINAMPTNRYWAQKQGLEPCDFYNFYIPGGDASEEEMM
                     KLVPRGLIINRFWYIRTVDAKAGELTGMTRDGVLYFEDGKIRHAVNNLRFNEIPHVAT
                     RRVLAMGPNRLASSGMSLPSILFDNFNFVDKTNF"
     gene            8576..8746
                     /locus_tag="PLO35_07130"
     CDS             8576..8746
                     /locus_tag="PLO35_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOH79478.1"
                     /translation="MADRTLVCKDCGKEFTFTDGEQEFYKEKGLQNEPQRCPDCRKAK
                     KAAFNRNRAPRN"
BASE COUNT         2020 a         2437 c         2583 g         1951 t
ORIGIN      
        1 ccgttccaca tcaccttgtg atagccggcc ggaagagacc cggaatccag ctcgcgcacc
       61 agctggcccc tcaggttata gatgctgagg cggtaatccg cctgctgttt gagcccaaag
      121 cggatggtgg tatccggatt gaaggggttg ggatagatgg atgaaatgcc agggatgacc
      181 ggcactccgg gagtgctgcc ctccggcagg gtatagactg ccaggacggg tccgtggagg
      241 tgagagctgc catccatttc cagatgttcc agccagtaat agtattttcc gctctggcag
      301 agctcggtat ccgtgaaggc gtagagctgt tgctgggagg tgttggtggc catgataaag
      361 gcttccaggc gaatggccct ggcaaagtcg tcgctctcgc cccggtagat ctgatagccc
      421 aggagattgg tctcggattg ggtaacccag agcagttgga tctggaagtt gcccgtgatg
      481 ctcacagtga agctggaaag ctcgaccggc agggtggaat cgtcggtatc cggaaacacg
      541 atttccaggc cctctccggc tttggcagct ggcaccagaa actctacctg ggtagtggtc
      601 cacaaagatg actgggcggg agtaacgaag ctgaagttgc cgctcagact gcggtaggcc
      661 aggtttagag gcagataacc caggttgtgg gtaatccgga cggtggcgcc tgccatgtcc
      721 actccgttta ccagcacggt gagcccggca ttttcgatat agatttgggg ctgggcatag
      781 cccacgctgc cggtcaccag gaggttgctg gcttctggca accagccttc gatctcgact
      841 gtgagcggga tcaggttcat ttcgtcctgg atattctgct gcatggtaat cttgaaatag
      901 ttgtcccctt gcaccgggtc tcccacatag tcctgataga tctcggtgtt atcggccttg
      961 attttcacgt cgtcaatgcg cagggcaagc tgctttgtgc agttgtaatg ccggaaggca
     1021 aaatagacgg tctggccatc ccaggcgtcc agattgaagg cccggtattg ccaggcgtcc
     1081 gtagtgaggg tttcctcggt gagggtggtg gtaaattggg agggatcggg atcgccggtt
     1141 gagatcagca gggcgtaatg ttccgcgtac caggtatctg agtaggcgcc gatccaatag
     1201 ttgaggctgt ctccagcgct gatggtgatg ggcggagaga tcagccagtt gtccggattc
     1261 aagaccagtc tgtctctggc ggtcttggcg gctttggttt gcatagagga ggtggcatcc
     1321 gcctctagcg cttccagcct gagagaggaa gctttggcgt cgggagccca ggattgggaa
     1381 accgccagcc agtttccgct tttggccatg gagggatcgt tcaccaggag ccagggatag
     1441 ccgtctccat tgttgtcgcg gcggacccaa ccctgcacct gaaactccgt tccgccctca
     1501 tagctgggtt caaaaccttc catatagggc ttttgggtga tggtgttatc ggtggtgctg
     1561 ccgctgatgg caacggtgtg gatggctttg gaaaggttgt ccgtcacgct gagggtggcg
     1621 ctgtgagagc ccgccctggg gctgttgtaa tagaccgtga agctaaagct ttgtcccgct
     1681 cccaaagtga tgggcagagc gggaagatct ttgagcttga aatacgcgtc tccgctgatc
     1741 gttatcccgt cggccaccac ctgcatggag ccgcctccgt tgttggtgac ggtgaaaagc
     1801 tgctcccggg tgtaattgat aaagaaatcc tcaaagacat aactggaagg gctgatatga
     1861 aactggggct ggccatagtc tcccgccaca aagttatcca cctgaaagct gaaggcttta
     1921 ttggacacac actggatggc gatatagaca ttggtgtttt cgcaggccgg ggggagagta
     1981 tagacgtagt tcgtccagga agtgggggcc tccacatagg tgccagcgct gccggccagg
     2041 tagtttgtga agtctgaata gctgtttccc gtggtggacc acaacacctt aaagcgctcc
     2101 aggccatagg cattggtgat ggagcgggca tagaagctga tcaccgggtt ttggccaaag
     2161 ctgatggcgg gtgagatcag ccagtcgttg ttggcaggag tggtggcagc caggcaggcg
     2221 gcatatctgg cgccgtcata ggcattccac agagcactca gaggaggggt ggtggcgctg
     2281 ggattgaagg cgatgtagga gccggtgtag ccttggttga ggaagctgac accgctgatg
     2341 gagtaggtgg cgctgccgtc tccgtcatac tgagtccagc caggcagcgt gaggctaaaa
     2401 tccgtggcgc tttcaaagcc gtcccggaat ttctcaatag caccgtgacc gctgagcggg
     2461 atgctataga ggtttttgcc cagatcatcc tggatattga gatcgctggt tttggcaccc
     2521 acaaggtcgg gccggaaata cagcgtgacg ctcacgtatt cgtctctggc caagccgatg
     2581 ggatagctat tggcatccac cagctcaaac tggtccgcgt cccctcccag gatctccatg
     2641 ccctgcaggt tcaaagtccc gccacccaga ttgtaaaaac ggaattcccg ggccggggaa
     2701 ccgtcttcga ggtctgtgac gccaaaggcg atggaggttt cggagacgcc tggaatcggg
     2761 acagcgactt gcacggaaac gtcatccaga tatatgcccc attgattagc cgcgctgaag
     2821 gcatgccagc caaggtaata tataccggtg ctgggcacgg taaaatactg ctccgcatcg
     2881 taatagacag tgtggacaaa tccgtcgtcc agatagagct gggtgccagc actcaaggcg
     2941 acggcgctgg cggaagcggc gatattcaaa cgcatgcttt cgggatatgt agcgcttcgg
     3001 gcccgatagg cgaaaccggc aatgtagtcc gtccctgcct ccagatagag gcctggagat
     3061 accagccagt cattgctggc ctggctcgcg ttgtagttta tatacatggc gttgggactg
     3121 gaattggggg tagtggtttg attgtcccag gtataggcat cggcgttgac gttgaggacc
     3181 gtccatcccg agggtagagt gggggctgta acagagtcga agttttcgtt ccaggggtag
     3241 cttcccacat aagtagtcgc atttgtgctg actcctgagg agtagtcata gaccgcgtca
     3301 taggaccaga tccggtaata atagctggta ttggagctca gggagctgtg gttgaatgcc
     3361 gtcagggagc ctgtataaag cactgtgccg ctgccgccga tcgtggaacc caggttatag
     3421 gaactgccgt tgacgggggt gccaaagctg tttgtgctgt tccaggctac catcacatcg
     3481 ttgttggcgg cgtttttggt ccagccaaga tcgatctggg aggcgctgac gggagtggcg
     3541 gtgaagctgg tgggatcggt aacggtagag gcgatgatgg tgatctggat attgggacag
     3601 taggaatcta cggttccgtt tccggtgggg actgatccat cctgggccca gtattggtgg
     3661 ctgttgttgg aattcgagta acggaaggtg ggataactga ctgttccact accgccatag
     3721 ttggtctcac agagcaccag caggttgttt gcggagtagt tgaaatccgt gatgtcaatc
     3781 gtcagccatc ctgatggagt aaatgtcctt gaagcgttat agacctctgt tgctccgctt
     3841 atgatgttcg cccaggtgga actggtcaga gtgttggtgc tggtggtctt cagatagatt
     3901 ttggtgggta cactggcgct ttgagcggtg cccacatacc atcccaagtg tgtgatcgtg
     3961 cccagggcat ttatatcgct actgcgataa atagccgcgg aacgttcgta accataccaa
     4021 gccccaaagg gttggcgctg cagattggtt ccggttccta tcgtcaccgt ggtctgggct
     4081 gtcagccagc caaagctgat cagcagcacc agcagggttg taagtatata cttgctcata
     4141 taatcctcta actacttgaa attggccgcg aaaaccgggt gacaggctca gccctgagta
     4201 gtttatgact tcttttggca caccaaagct tatccgttcc cgcatgatcg ttaatcatca
     4261 cgatgcagcc ttgcgagtcc cagaaaaatg ttaggctact ttttgtcaag tgttacgcta
     4321 agtatctgcc atagtaacac gctttctacc agtggaaata tttcaacacc gcaaggaccc
     4381 cggggcagcc cttgcgtgta atgctccatc cccttatctt gtcggcaagg agacgattat
     4441 gatcacatac acagatgccg cgcgagcgtg gatggacggc ctggatgata aagaccggca
     4501 gcgcctgggc cccgtattgg agctctttga cgcgcatacg gaccaggctt ccgcggatta
     4561 ctaccacccc cacatcctgg tgctgaagct gaaaggccag cccgccctgg cactttcgcc
     4621 ccgcaagcag tatctgagct tttacgcctg gtccatgcag acgatgggca tcctcaaagc
     4681 cgctctgcca aagctgggaa aggccaatcc gggggtgggc tgcctgcgct ttcgcaaagc
     4741 ttccgacctc aatatggaca ccctggccga ggtgttcaaa cagatgaacg cggtggagca
     4801 cgaagcggga tttggctttc agtgaggcag caggggagcg gcaaaggtct cgttgtttct
     4861 ttggttacgc attacccgtt atgcgttatg ctggcgcgca tggatacgct tgatgcaatt
     4921 tgtaaatcct cctctgcttt ctcctctgtc tctgagtatt gtcgagagcg aggcactcac
     4981 cggattggaa tcagacgcta cagccaggta accgaggacg ttggcgggcc cgttatcgat
     5041 ctaaaaactt gacacttccg ccccgcctat tttccttgtg aaaattctat cccgctcaaa
     5101 ggagcaaaca tgctggaaaa caattacaac caggaaccgc agcgtcgttt tcagtatccg
     5161 ggagtgatgg aagctcccgt ggtaggcgtc ttcgattggg tggtgatcat gattttgctg
     5221 gccctgccga tcgtgaatat catcatgctg atcgtgtggg ctaccgatca acagggcaat
     5281 cccaaccgcg ccaattttgc caaagccacc ctgatcatca tcgccgtgca gatcgtgctg
     5341 atcgcggtgt tggtgggcac gatgatgggc accgtcatgg cctttgccga taagtttgcc
     5401 tgagctggaa tgacccgctg gagttcaagc ggccaggata tctgtgtgca tgacaacaag
     5461 tatgcggacc gcttggactt caaccagcgc gccgaaggag agagtgcttg ctgctaccgc
     5521 gctccgaagt actgcttgaa gtccaaagcc ccctcaggta aagggataaa agcataacct
     5581 gtttaggggc tttgaactcc aagtgatgcc aatcccctcc gcgggaatga caagttaacc
     5641 actccatgag agaaatgaga gaaaacagag atggtaaacg gggacgttta ccgctccatg
     5701 atataaggag atgccatgcg ttatcttgac ctggccatcg gcgaggccct caatctgggt
     5761 gccgagtatg ccgatatcag aatccagcgc accaccgatg aagtggtgct gctgcgcaat
     5821 ctgagcctca aaaacacctc ccgggacgtc ctgcacggtt atggaatccg tgtcttcaag
     5881 gacggcgcct ggggctttgc ccaccaccac gtcttcacgg aagaggccgt cctggccacc
     5941 acccgcaggg cctatgagat cgcggccctt tccgcttccg tcaatcaaaa tcacaagctg
     6001 cgcctggccc cggaacgcgg ttacctcgcc agctacaaga cccccctcaa aatcgacccc
     6061 tttgaggtcc ccctgggcca gaaggtggag ctgatgatgg aagtgaaccg tgtgatgctt
     6121 ggctacgaag gcatccgcca ggcgatgttc tatctgatct cccacaagga cgaaaagctc
     6181 tttgcctcca cccttggcac ccggctggaa ctcaataccc agtggatcga tccgatggtg
     6241 atcgccagtg ccgtccatgc cggcgacagc caaagccgca cctttgacga aggtggcagg
     6301 gctaccggtt gggaatgggt gacggagctc aacctgcagg aaaaagcccg ccagatcgct
     6361 gaagaagccc tgatcaaggt caaagcggac accctgggcc cagaagaacg ccgcagcctc
     6421 atcctggacc ccaatcacct gggcctcacc atgcacgaga gcgtgggcca tcccaccgag
     6481 ttggacagag tgctgggctg ggaagccgac tatgccggga tttcctttgc cacccctgaa
     6541 aagctcaaaa actaccgcta cggctccgag atcgtcaact ttgtgggaga caacaccctg
     6601 gaccagggac ttgctaccct gggcttcgac gacgacggtg tgcccggcca gaaatggcac
     6661 atcatcaaag acggtatcct caacgagtac ggcagcaccc gcgacaccgc tctggagata
     6721 gggctggagc actcccgggg ctgcaaccgc gccacctatt actttgacca gcccatcaac
     6781 cgcattccca acctctatat gctgcccggc acccagcccc tcactccttc ccaactgatt
     6841 gccgatacgg aagatggcgt ctatatccag ggccgcggtt ccttcagcat cgatcagcac
     6901 cgggtcaact tccagttcgg aggagacttc ttctgggaga tcaaaaacgg caagctggcc
     6961 cgtcctctca aaaaagtcat ctacaaatcc tgcaatccgg aattctggaa cagctgcgac
     7021 gccatctgcg acgagcggtg gtggcgtccc ttcggagtgg tcaactgcgg caaaggccag
     7081 ccttctcaga cggcccgcat gacccatggt tccgcgcctg caagattccg caacatcaga
     7141 gtgggaggtt cccaatgaac agcagcttcg aacgcatcgc ccaatacatc aaaactcact
     7201 gccaggctga tgattacagc ctcaggatct tccatgccga cagccatgag acccgctttg
     7261 cccaaaaccg cgtgacccag catctggccg gacccaagct gcgcgtgaac ctcaacgtgg
     7321 ctttcgataa caagaccggc agcgcgtctg tcaaccagga cgatcccgag gccctggccc
     7381 gactgatcca gagcgccgag gccatcgcca ggctcaacca gcccgatccg gaatacatct
     7441 tcagcgctcc ccaagcgcag ctgccagagg tccaaaacgc cgatcccgcc acccgggacc
     7501 tcgatcccgc caggatggtc cagatcgtcc aaagcatgat catgaaagcc aaagccatgg
     7561 acgccaccgt ctccggcatg accgaaaagc acttcagcga gtgggccctg atcaccaaag
     7621 gcggctttga aggctatgac gccagcaccg agtttggcca ttccatgacc ctcaaaaagg
     7681 aggaagtgga gaccaaggtg agctatgagg ccaagtcctt tgccggtttc gagccggacg
     7741 cggaatttga gaccctcaaa gcccaggccg ccagcctggc ctccaaacag gtgatggaac
     7801 ccgaaaagat cgccgtcatc ctcaggcccg gcgctctcat ggaactgctc tggttcatgg
     7861 gctggatgat gaaccgccgg cagagcgacg aaggcttcac gcccttcaca gaccagatgg
     7921 gcaagctctt cttcggcgaa aacttcaacc tctacagcac cctgaaacgc acggacatca
     7981 aggccagccc cttcaactcc gaggggatcc cctcgcggga gatcgcctgg gtggagaagg
     8041 gcgtgatcaa tgccatgccc accaaccgct attgggctca gaaacaaggc ctggaaccct
     8101 gcgacttcta caatttctac attccgggcg gcgacgcgtc ggaagaggaa atgatgaagc
     8161 tggtgccgcg cggattgatc atcaaccgct tctggtatat ccgcaccgtg gatgccaaag
     8221 cgggtgaatt gaccggcatg acccgtgacg gagtgctcta ttttgaggat ggaaaaatcc
     8281 gccacgccgt caataatctg cgtttcaatg aaatccccca tgtggccaca cgccgtgtgc
     8341 tggccatggg cccgaaccgt ctggcgtctt ctggaatgag cctgccgtcg attttattcg
     8401 acaacttcaa tttcgtggac aagacaaatt tctaaaaaaa cagttgacag gaattcacga
     8461 gctataaatg tgttcgcatc agagcgattt aacattccca tgagctttcc gcttgttttt
     8521 tggcgtacct cagacccgaa tgtccaaatc ctcccaaaaa acataggaga gacccatggc
     8581 cgacagaacc ttagtctgca aagattgtgg caaagaattt acgttcaccg atggcgaaca
     8641 ggaattctac aaagagaaag gcttgcaaaa cgagccccaa cgctgccctg attgccgcaa
     8701 agccaagaaa gcagccttca atcgtaatcg cgcgccccgc aactaatata tagtccgcgc
     8761 aaatgccttg gcccccgcag ataatgtggg ggcctggttt tttttgcgtg atagggttat
     8821 gcgttatgcg ttacgcggct ggcgccgctc tcgctaatgg atccggaaac gtcctcgttt
     8881 ccgcgtgtgg ataaccctca gagactgttg cacattaccg tatcactggc tttagagctt
     8941 gtgaaaaaac tgattatcgt ggcattattc cttacgagtc ccagcgggac g
//
LOCUS       DAOSFN010000103         8974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6614_length_8974_cov_8.969728, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000103 DAOSFN010000000
VERSION     DAOSFN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6614_length_8974_cov_8.969728"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..862)
                     /locus_tag="PLO35_07135"
     CDS             complement(92..862)
                     /locus_tag="PLO35_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH79479.1"
                     /translation="MNIPFNLKRMLDTELHFPQRFTNMVQKNYGLIYFNEGNKNSWDS
                     NHAVITDLIGVESSVRDIESFYKSKGINPRVFCSYQQGELDKLRPSLENHEYTIEMRP
                     DKFFVHDHESALKPVPGMRIQRVRNLTIDIMETIAMEFGGDFTIRVAERHLQHPSYHL
                     LCGYWEEEVVSLASVGIFAGYSRVDDVYTRSAYRGRGFAGAMMHYLIEYHRQLSQNYL
                     YLYSSHPESIRVYQKAGFSEVPLDMPSWMAHKELQVFG"
     gene            complement(1082..2845)
                     /locus_tag="PLO35_07140"
     CDS             complement(1082..2845)
                     /locus_tag="PLO35_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOH79480.1"
                     /translation="MKRFISLFIILTGLAALSAMPVDEISSSRVLQAQLQRMGCPHAR
                     VSHQETLYHQETALARAYELDSGGYVVVSADDILPPVIAYSIHDSFGPSEDNLLADLI
                     RADLASRLQAHHQGLYSEDHQAAWQNLLSSASVRDFQQWPPDGYSTTGGWCKTLWNQT
                     APYNNLCPMDPVTNQRSIAGCPAVAIGQIVNYHETLNGTVFSDADDYHHNYSGRNYWI
                     DDDAANLSFPSFPQLNTYLASLQHNWNYQQDQLATDRAALIFACGVAAGQVYSSQASG
                     TFAVSQAYAAIQRFGFSGSQLITDPESNYRDLMAQNMMNAQPALLAVVTPAWDAGHNV
                     VVDGYNTDGFFHVNFGWGGSYNGWYQIPSGIPYNLTVLEGVVLDIQASQPLFNLPEEI
                     VFSDNNHVQAELINISANPVEIEAIQVRPGAFNPDPYFLITLSHAPLPYILGVGQSLL
                     IGINLVEGDNAPREIITLDLAVIHATGVLHIPIRVDTTFLSPNADEHIPPLQSIRLGP
                     NPFQNRLTIRTEGEIPSQACVYDLRGRKLQELSIEPASPTQASFMWDGRDMAGHSCPA
                     GVYLVRVKSGSGCRVLKVLKL"
     assembly_gap    2959..3058
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3066..3626)
                     /locus_tag="PLO35_07145"
     CDS             complement(3066..3626)
                     /locus_tag="PLO35_07145"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOH79481.1"
                     /translation="MKAHILLICGFVCLATLLCAVENPALPSLRLEEKPSFHLMGKSL
                     RDNRDPGVLLMLWLDFFKTASIVPKPIPGVEYGLYAYGDGLSPTTDQQVIYLAGYEVP
                     SLNIKPSLLNATTIPAAKYAVFEHVGSFTNILGTIDSIFSTWLPAHNLTPAAQFYFER
                     YDEDSKIESESTVIEIWVPVELKNSN"
     gene            complement(3772..4335)
                     /locus_tag="PLO35_07150"
     CDS             complement(3772..4335)
                     /locus_tag="PLO35_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79482.1"
                     /translation="MKPNSLKMFIMLVAILAATTLAAQTDTAFSPTIKHRRGSLGIGY
                     GLCYAGLGFNGDYNINDDIAVSGSIGTFGYVGGWELGGKYYFGQFDSTFRPMLSAWYG
                     VNAMIVARPDNPGLPDVTEAHHGFCLGAGGQWMFSKSKRHGFDAGILYILTTTQAKRI
                     EELESQGYGTFSKGSRFLFSFGYRYAF"
     gene            4678..7749
                     /locus_tag="PLO35_07155"
     CDS             4678..7749
                     /locus_tag="PLO35_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH79483.1"
                     /translation="MSQTQIVEYSHQFAKAVASMWNASGEGWNGRVFNSSEARVCQEE
                     SSTRYLNLYLSVEGERVLGYAKLTDYTEEPGVAYIELLNVIPSHHGKGIGRDLVKRCV
                     LRAAELGYERLDLFTWAGNTKAVPLYKKCGFFWEKMENNSTHLMNFLPGLLKLAILKP
                     WWDVFDWYEDCKRVLDTEPDGRDEHGFDIYDYSWEKDGRRLEISFERSGRGIVAIRTP
                     DFQIAISTEQPKPVFGIPRKLRYSLSNLSGKALEIRLDGLNDAGISHSFQHSGLLESS
                     LELESEYLPNPLDKEVSEWETSPRVRCMITLDGHAFSMAIGLRVQYPLTATLRNVDSL
                     LQPGLWYKMYLNLESHFDRDCYFEIRLVEHEHICLRESEFSFTSQAEGRCWLELEFSC
                     DQALVYNPKIRVEATPEGGSALDFCLYPSLILHQPRGRHGSENPDASFMVNSLNSYRS
                     YKEGSKNFAWVGRLFCNGISLMCPQPGKPYSEELESALPYQTLFNQGPDYIETENRFR
                     SKDIYGLEFALIYRFWDSGLVEVFPRCLALPEDRDEIWLKLPFNIYEGRLSFWFDGRI
                     VETDKELLDFGCCDLPRACPQENWLFSETDDASIGLIWSEDTRLTPDRYWLAWEVDLK
                     ALAAKGLDAPQPLQIHLDVFKNSWQLRNLARGRVPTQPISSGVELRANQGDPVLKLPC
                     RAELIQNLDMELKATYSLESSCVSKPAVLDSPVNEDGIRRLSWDLTQAPDQPLELVKA
                     SVRMPYTDIERSQLLLHPRGESSYREDAQLLSFDNGQITITAARDARLPGLISLKHKG
                     LEYLDNAYPDYAAKGFYNPFPGGLSLTPADVSKRKQLEELHTVEAASLTDQFGTLWQG
                     LALSTRVEQFGPQKGLVFRQFYLALPGVPVLMLLGEISDWGGRRQYYNFNLSLYRQLG
                     HKRGEDGYLLGHKDGRQQRIHCSATESYASAWLDTVAITSPQNPSHLQFQTLGSIWAA
                     ITQDPCMSVIQLRLYTRVSDPLPQRTEPLFLIFSPRIYQKQELGQLLGLEV"
     gene            complement(8049..8876)
                     /locus_tag="PLO35_07160"
     CDS             complement(8049..8876)
                     /locus_tag="PLO35_07160"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79484.1"
                     /translation="MKQLKALICLTAMLGVAGCLVAQQADGVAKEVPKLPEIVPVEGG
                     TVTRGDNMLSVAGFQISLNEVTQAQYELIMGSNPSLLRDPAHPVENVSWLDAVEYCNR
                     LSIKSGLTPVYTFKGAKSDPDGWPKGWKTDSSSHNKISVFGIANGYRLPNDYEWEFAF
                     LGGNKYSGYRFSGGNKLEELGWYIANSDSTHQTVGLKSPNELGLFDLCGNVREWVGEK
                     LKSGDARALGVDRDAVRIVRGSGYLSPEEDCGAGIKTWHDPLYKAPDLGFRVARKLK"
BASE COUNT         1974 a         2384 c         2491 g         2025 t
ORIGIN      
        1 acgaggacgt tttcggttcc ctaacaattg caatctctgt tcctctatag tctctgtctc
       61 tctgttgctc tgttttcaaa cagaaaatgc cttatccgaa gacctgcaac tccttgtggg
      121 ccatccaact gggcatatca agcggtactt cgctgaagcc ggctttctga tagacgcgga
      181 tcgattccgg atgagaggaa tagaggtaga gatagttttg ggaaagctgg cggtgatact
      241 cgatcagata gtgcatcata gcgccggcga aacctctgcc gcgataagcg ctgcgggtat
      301 agacatcgtc cactctgctg tagccggcaa agatacccac cgaagccagc gacaccactt
      361 cctcttccca ataaccgcag agcagatgat aggaagggtg ctgcagatgg cgctcggcca
      421 ccctgatcgt aaagtctccg ccaaactcca tggcgatggt ctccatgatg tcgatcgtga
      481 ggttgcggac gcgttgtatc ctcataccgg gtacgggttt gagggcgctt tcgtgatcgt
      541 ggacaaaaaa cttgtccggc ctcatttcga tcgtgtattc gtggttctcc agtgagggcc
      601 tgagtttgtc cagttcacct tgttggtagg agcaaaagac gcggggattg atgcctttgc
      661 ttttgtagaa agactcgatg tctctcacgg atgattccac cccgatcagg tctgtgatca
      721 cggcgtgatt gctgtcccag gaattcttgt tgccttcgtt gaagtagata agcccgtagt
      781 ttttctgcac catgttggta aagcgttggg ggaagtgcaa ttcggtatcg agcatgcgtt
      841 tgaggttgaa aggtatgttc atgttttctc ccttgggccg tgatacttta ctaacattat
      901 acgcgggttt tggctttggg caaagtgaag ttgcagaccg ggcctgtaca cccgatagag
      961 cagagcgcgg atcgcaggat gacaggattc acaagatccg caaagtggaa tggcgaggtc
     1021 cgcgtgagtc tgtgatctcc tgcccggtgg ctttatctga gcattctcaa atcttgatct
     1081 atcagagctt tagcaccttc aagaccctgc atccggagcc gctctttacc ctcacaaggt
     1141 aaacaccagc ggggcagctg tggccagcca tgtcccgccc atcccacata aatgaggctt
     1201 gggtagggct tgccggttcg atgctcagct cctggagttt acggcccctc aggtcataga
     1261 cgcaggcctg ggatggaatc tcaccctcgg tccggatggt gagtctattc tggaaaggat
     1321 tgggccccag acggatagac tgaagaggcg ggatgtgctc atccgcgttg ggactcaaga
     1381 aggtggtatc cacccggatg gggatatgaa ggactccggt ggcatggatg acggccaggt
     1441 cgagcgtgat gatctctcgg ggagcgttat cgccctccac cagattgatc ccaatcagca
     1501 ggctctgtcc gactccaagt atataaggca gaggagcatg cgacagggtg atgagaaagt
     1561 agggatcggg attgaaggca cccggtctga cctggatggc ctctatctcc accgggttgg
     1621 ccgaaatgtt gatcagctcc gcctggacat ggttgttgtc actgaagacg atctcttccg
     1681 gcaggttgaa aagtggctgg gaagcttgaa tatccagcac cacgccttcc agcacggtga
     1741 ggttgtaggg aatcccggag gggatctgat accagccgtt gtaagagccg ccccagccaa
     1801 aattgacgtg gaaaaagcca tcggtgttgt agccatccac caccacattg tgacctgcat
     1861 cccaggcggg agtgaccact gccagaagcg ccggctgggc gttcatcatg ttttgggcca
     1921 tgaggtctct gtagttgctc tcgggatcgg tgatcaactg actgccactg aaaccgaatc
     1981 tctggatagc cgcgtaggcc tggctgacgg caaaggtgcc gctggcctga gagctgtaaa
     2041 cctgcccggc cgccacgcca caggcaaaga tgagggcggc ccggtcggtg gccagctggt
     2101 cctgttgata gttccaattg tgctggaggc tggccagata ggtgtttagc tgtgggaagc
     2161 tgggaaagga caagttggca gcgtcgtcgt ctatccaata gttgcggccg gaatagttgt
     2221 gatgatagtc gtcggcatcc gaaaacacgg tgccgttgag cgtctcgtga tagttgacta
     2281 tctggccgat cgccacggca gggcatccag cgatgctgcg ctggttggtt accgggtcca
     2341 tggggcagag gttgttgtag ggggccgtct gattccagag ggttttacac cagcctcccg
     2401 tggtggaata tccgtccgga ggccattgct gaaagtctct gacgcttgct gagctcagca
     2461 ggttttgcca ggccgcctga tgatcttctg agtagagtcc ctggtgatgg gcctgcagcc
     2521 tggaagccag atcggccctg attaaatcag ccaggaggtt atcttcggag gggccaaagc
     2581 tgtcgtggat ggaatatgcg atcacagggg gcagaatatc atccgcgctc acaaccacat
     2641 atcctcccga gtcgagctca taggcccgcg ccagggctgt ctcttgatga taaagcgtct
     2701 cttgatgact gacccgcgcg tggggacatc ccatcctttg taactgggct tgcagcaccc
     2761 gcgaggagct gatttcatcg accggcatcg cgctcagcgc tgccaatcct gtcaggataa
     2821 taaacaagct gataaaccgt ttcatggcat tatctccatc gcttagataa caaattggct
     2881 gaagcaagct ccgcgccaaa agccggattt acttgtaaag ataagctctc cggggagtag
     2941 agcaaagtgg gcagcggcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     3061 cagcttcagt tcgagttttt cagctccacc ggcacccaga tttcgatcac ggtgctttcg
     3121 ctttcgatct tgctgtcttc gtcataacgt tcaaagtaaa actgggccgc gggcgtgaga
     3181 ttgtgggccg ggagccaggt gctgaagata ctgtcgatag tgccgaggat atttgtaaat
     3241 gaaccaacgt gttcaaacac agcgtatttg gctgccggga tcgtggtggc gttgagcagc
     3301 gagggcttga tattgaggct gggaacttcg tacccggcca gatagataac ttgttgatcg
     3361 gtggtggggg ataggccgtc accataggca taaaggccat attccactcc ggggatgggc
     3421 tttggaacga tggaagccgt cttgaaaaag tccagccaca gcatcagcag gacgccggga
     3481 tcacgattgt cccgcaagga ctttcccatc agatgaaaac tgggtttttc ctcgaggcgg
     3541 aggcttggca gggcgggatt ctcaacggca cacaacaggg tagccagaca gacaaaacca
     3601 catatcagca ggatgtgagc tttcattcag gactccagtc aatggtttgg gagactgttc
     3661 ttttgcggtg ccgatcacgt caagtgatat tattgcgtga tccggatctg cccttcagga
     3721 ctgggaagag aacctgttat tgataaagag ttgttctaat cagcccggct gttaaaacgc
     3781 gtagcggtag ccaaaggaaa agaggaagcg gctgcccttg gaaaaagtgc catatccttg
     3841 ggattccagc tcttcgatgc gcttggcctg agtggtcgtg aggatgtaga ggatgccggc
     3901 gtcaaagcca tgccgtttgg atttgctgaa catccactgg ccccccgctc ccagacaaaa
     3961 accgtggtgc gcttccgtga cgtccggcag gcccggatta tcaggcctgg ccacgatcat
     4021 ggcgttcacg ccataccagg ctgacaacat cggacgaaag gtgctgtcga attggccaaa
     4081 gtaatactta ccgcccagtt cccagccgcc cacatagcca aaggtgccga tgctgccaga
     4141 aacagcaata tcgtcgttga tgttatagtc accgttgaag cccagaccgg catagcagag
     4201 gccgtatccg attcccagag agccgcgccg gtgtttgatc gtgggagaaa aggctgtgtc
     4261 cgtctgggcg gccagggtcg tcgcggccag aatggccacc agcattataa acatcttcaa
     4321 gctattgggc ttcatcatta ctccctgagc gatttttatc acaaaatgcc gaattgctgt
     4381 gcatgtcaag tgaaatcgca acagactgac ggaggaactg gtgccaaacg cggtatggac
     4441 cctcactgaa gacttcagat ctcttcacca caacttcaaa cgcttataca gaagccgtat
     4501 ctatggactt caacccgctc atcacacagg attttgtgat gcctgccccg gaagtagctg
     4561 gaaagttttt taccccttac cggtgacagc ctgaaaagat ccaacataat ccttgacaca
     4621 atgtatctcc agcaaaaaac gtcccacaca ggcaaatcaa cgcgcaaggg agtcacgatg
     4681 tcacaaaccc aaatcgtgga atattctcat caattcgcca aagccgtcgc gtccatgtgg
     4741 aatgccagcg gcgaaggctg gaacggccgc gtcttcaata gcagcgaggc cagagtgtgc
     4801 caggaggaat ccagcaccag atatctgaat ctctatctga gcgtggaggg cgagcgcgtg
     4861 ctgggctatg ccaagctcac ggactatacc gaggaaccag gtgtggccta catcgagctc
     4921 ctgaacgtga ttcccagtca ccacggcaaa ggcatcggac gtgatctcgt gaaacgctgc
     4981 gtcctcaggg ccgcggaact gggttacgag cgtctggacc tcttcacctg ggccggcaac
     5041 accaaggccg tgcccctcta caagaaatgc ggttttttct gggagaagat ggaaaacaac
     5101 agcacccatc tgatgaactt tttaccgggt ctcctcaaac tggctatcct caagccctgg
     5161 tgggatgtct ttgattggta tgaagactgc aaacgggtgc tggataccga accggacggc
     5221 cgtgacgagc acggttttga catctatgac tatagctggg aaaaggacgg ccggcgcctc
     5281 gagatcagct ttgagcgcag cggacgcggc atcgtggcca tccggacccc ggacttccaa
     5341 atagcgatct ccactgagca gcccaagccc gtcttcggaa ttccccgcaa actgcgttac
     5401 agcctctcca atctgagtgg aaaagccctg gagatcaggt tggacgggct caacgacgcc
     5461 ggtatcagtc atagttttca gcactcaggc ctgctggaaa gcagcctcga gctggaatca
     5521 gagtatcttc ccaatcccct ggacaaagag gtgagcgagt gggaaaccag tccccgcgta
     5581 agatgtatga tcaccctcga tgggcatgca ttttcgatgg ccatcggcct gcgggtgcag
     5641 tatccgctca ccgccacact gcgcaacgtg gattcgctgt tgcagccagg cctctggtac
     5701 aagatgtacc tcaatctgga gagccatttc gaccgtgact gctattttga gatcaggctc
     5761 gtggagcatg agcatatctg cctgcgtgag agcgagttct ccttcacttc ccaagctgag
     5821 ggccgctgct ggttggaact ggagtttagc tgtgaccagg ccctggtcta taatcccaaa
     5881 atccgggtgg aagccactcc tgaaggagga tcagccctgg atttctgtct ctatccctcc
     5941 ctcatcctgc atcagccgcg gggcaggcat ggcagtgaaa acccggatgc cagcttcatg
     6001 gtcaattccc tcaattccta tcgctcctac aaggagggca gcaaaaactt tgcctgggta
     6061 ggccgtctgt tttgcaacgg catctcgctg atgtgtcccc agcccggcaa gccctacagt
     6121 gaagagctgg aatccgctct gccttatcag accctgttca atcagggccc ggactatatc
     6181 gagaccgaaa accgctttcg ttccaaagac atttacggct tggagtttgc ccttatctac
     6241 cgtttttggg acagcggcct ggtagaggtt tttccgcgct gcctggccct tcccgaagac
     6301 cgggacgaga tctggctcaa actgcccttc aacatctacg aaggccgttt atccttctgg
     6361 ttcgacggac ggattgtgga gactgacaaa gagttgctgg attttggctg ctgcgacctg
     6421 cccagagcct gccctcagga gaactggctg ttttctgaaa ccgacgatgc cagcatcggt
     6481 ttgatctggt ctgaggatac caggctgacc ccggatcgct attggctggc ctgggaagtg
     6541 gacctcaagg cgttggcagc aaagggcctg gacgctcctc agcctctgca gattcacctc
     6601 gacgttttca aaaactcctg gcaactgcgc aatctggcgc ggggtagagt cccaacccag
     6661 cccatcagca gcggtgtgga actgcgggcc aatcagggtg atcccgtttt gaaactgccc
     6721 tgccgggccg agctcatcca gaatctggat atggaactca aagcaactta cagcctggaa
     6781 agctcttgcg tgtccaagcc agcggtgctg gacagtcctg tcaacgagga cggcatccgc
     6841 aggctgagct gggacctcac gcaagcgccg gatcagccat tggagctcgt aaaggcatca
     6901 gtaagaatgc cctacacgga tattgagagg agccagctgc tgctccatcc ccgcggcgaa
     6961 agttcttacc gggaagatgc tcagctattg agcttcgata atggccagat cacaatcacc
     7021 gcggcccggg atgcccgctt gccgggattg atatctctga aacacaaggg cttggaatat
     7081 ctggacaacg cctatcccga ctacgctgcc aagggctttt acaacccctt tccgggaggc
     7141 ctgtctctca ccccggctga tgtatccaaa cgcaagcagc tggaagagtt acacacggtg
     7201 gaagcggcca gcctcacaga ccagtttggc acgctttggc aagggctggc actgagcacc
     7261 cgagtggagc agtttggtcc ccagaagggc ctggtattca ggcagtttta tcttgcgctg
     7321 ccgggcgtac cggtgctcat gctgctgggt gagatcagcg attggggagg cagaaggcag
     7381 tattacaact tcaacctcag cctctacagg cagcttggtc acaagcgtgg agaagacggc
     7441 tatctgttgg gccacaagga tggaaggcag cagcgcatcc actgcagcgc taccgaaagc
     7501 tatgccagcg cctggctcga tacggtggca atcaccagcc ctcagaaccc cagccatctc
     7561 cagtttcaga ctcttggctc aatctgggct gccattaccc aggatccctg catgtctgta
     7621 atccagctca ggctctatac gcgcgtctcc gatcctctgc cccaaagaac tgagccgctg
     7681 tttctgatct tcagtccccg catatatcag aaacaagagc ttggtcagtt gttgggcctg
     7741 gaagtttaac cagttgtcag gcagcatgat atagccaggg gcaggacctg agcctctttg
     7801 atttacatca ccctgcagcc aggtttggca tgtcttcaga tcagaactgg ctcctgtgtt
     7861 gatgctcaat acctgggcta gcgttggcct gatccaccca atcggagact tatagagaat
     7921 ctggcctggg acatcttccc ctttcgccac ccatgcctat cccgtgccta tcccatgcct
     7981 atcccatgct taagcttggg ttaggtttgg gtgatgggtg agtgctttga acgaggctaa
     8041 gcggaagttt acttgagctt tctggctacc ctgaacccca aatccggggc tttgtagagg
     8101 gggtcgtgcc aggttttgat cccagccccg caatcttctt cggggctcag gtatccgctt
     8161 cccctcacga tccggaccgc gtctctgtcc actcccaggg ctctggcatc accggatttg
     8221 agcttttccc ccacccattc gcggacgttt ccgcacaggt cgaagagtcc cagttcattg
     8281 ggggacttga ggcccacggt ctggtgggtg gaatctgaat tggcaatgta ccagcccagt
     8341 tcctccagct tgtttccacc gctgaaacgg tatccggaat acttgtttcc gcccaggaag
     8401 gcaaactccc attcgtagtc gttgggcagg cgatagccgt tggcaattcc gaataccgag
     8461 atcttgttgt ggctggagct gtctgtcttc cagcccttgg gccagccgtc cgggtctgat
     8521 ttggcaccct tgaaggtata gacgggcgtg aggcctgact tgatgctgag ccggttgcaa
     8581 tactccaccg cgtccagcca ggagacgttt tccaccggat gagcagggtc cctgagcagg
     8641 gaaggattgg agcccatgat cagctcatat tgggcctgag tgacctcgtt gaggctgatt
     8701 tgaaaacctg ccaccgaaag catattgtcg ccccgggtca cagtgccgcc ttctactggt
     8761 acaatctccg gaagcttcgg gacctctttg gcaacgccat cagcctgctg tgcaacaagg
     8821 catcctgcca cgccaagcat tgcggtcaga catatcaaag ctttgagttg cttcatcgtt
     8881 cctcctgttt ttgtatattg gttgacgaat ccaagaaaaa tgatacgggt agggtttgtc
     8941 aagaaatagt catgctcgat ggcaggagtt ctgg
//
LOCUS       DAOSFN010000104         8840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6742_length_8840_cov_9.234377, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000104 DAOSFN010000000
VERSION     DAOSFN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6742_length_8840_cov_9.234377"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLO35_07165"
     CDS             <1..819
                     /locus_tag="PLO35_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOH79485.1"
                     /translation="VFVEQTEQLGTGHAVMCTADALKDDAGDVLILCGDVPLLSKETL
                     DRLVNHHRESSAACTVLTAFLDDPGRYGRILRDEQGMVSGIVEFKDATDAQRAIKEWN
                     TGIYCFRTSDMFQALGKISNQNQQGEYYLTDTLAILRSEGKTVASIPLERHIEVSGVN
                     SQQQLAELEDAFIASIREHWLNQGVVMHNPASIYIDDDVIIEPDVEIGPHCVIQGKTT
                     LETGVVIGPSCLIDSCRVCNDSILEGHNILSYSHIPEQHIVTFGEKVIEEEEYE"
     gene            812..1315
                     /locus_tag="PLO35_07170"
     CDS             812..1315
                     /locus_tag="PLO35_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOH79486.1"
                     /translation="MSERYLYSPWRIDYILGEKPEDCVLCRFKQEGEDKDNLIIWRGK
                     LAYVMLNRYPYNNGHLMIVPYEHKSCLTDLDVATLQELARLSQVTELVFKEQFHCEGI
                     NLGLNLGRAAGAGIEEHLHIHMVPRWMGDDNFMSVIGGRRVIPEDFTQSWSRLRSAFA
                     NHMMDKG"
     gene            1315..1791
                     /locus_tag="PLO35_07175"
     CDS             1315..1791
                     /locus_tag="PLO35_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOH79487.1"
                     /translation="MIQDLTENRDPKKLRSNSRVWYIVIAVATLLAAGYVIYRNYLHD
                     DNPLPPGEESMPAIKVIVSNGCGYESLATDYANYISKLNVEVVKLTDTPKPIYDKSLI
                     VVRKQDEQDLKRLQAMTGIQRFTLAVNEASEAPFIIILGRDYQQFMKENQGGTFGR"
     gene            1781..2167
                     /gene="rsfS"
                     /locus_tag="PLO35_07180"
     CDS             1781..2167
                     /gene="rsfS"
                     /locus_tag="PLO35_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOH79488.1"
                     /translation="MADNTKLNAILGWLNEKKAENISVYDVQKSSGYTDVIIVCEGAA
                     DLHNQAIANHLLDMAKANHFHVMSKEGMEFGRWVLIDVGDVVVHIFLSDTRSYYDIDK
                     LFKKVQETEDPSESISKDTPASEQTT"
     gene            2164..3816
                     /gene="argS"
                     /locus_tag="PLO35_07185"
     CDS             2164..3816
                     /gene="argS"
                     /locus_tag="PLO35_07185"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOH79489.1"
                     /translation="MIKDIIRDNIIEVLNAHGYTHEREFVVEIPNNQEFGDYSSNAAL
                     VLSKENKTAPKLMAETLIKDLKKNKFFKKVEMAGPGFINFHLSPALYQMMLWTIHGKG
                     EDYAATDLGKGEKVLLEFVSANPTGPLNIVSARAAAFGDTLYRIMKFVGYDPAREFYI
                     NDAGNQVDILAESLELRLRELQGERIGDFPYEAYHGEYVKHLAQRLNAVEGVRVFMMT
                     EKDRMDHLKEFALSELLEMQRLSLEKFGVVFDGWVSEKTLRAEGVVEEVLSYLTEADC
                     TYEKEDAIWFRSTDFGDDKDRVLMKSDGSITYFVPDLAYHLTKIQRGYSTLIDIFGPD
                     HHGYVPRLKAAFKALKYDEEMLEIIFLQQVNLFESGERVKMSKRAGKIVTMDDLVSVV
                     GKDAARYFFIARKANAHLNFDLELALKQNNENPVYYCQYAHARICSIVKKAKKDRVLP
                     KTYKKELALKLNKPEELALIRKLTDLPELLQLISIHREPHRLATWAEELCGLFHRYYN
                     KYQVVSAKNKELSQARLLLIDTVRMSLALAFDLMGISAPEKM"
     gene            3832..4269
                     /gene="rimI"
                     /locus_tag="PLO35_07190"
     CDS             3832..4269
                     /gene="rimI"
                     /locus_tag="PLO35_07190"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOH79490.1"
                     /translation="MAEFRRMRMEDLPRIYEIERLAFCDPWPDEAFSMMNGYHSYVAL
                     REGLLIGYIICLSALDESSIANLAIDPQFQRQGFGKELLRFAMDELATQGIKRMFLDV
                     RRGNLAAQGLYEQFGFVHVGVRKGYYSDPPEDALVMMWTKVTE"
     gene            4266..5864
                     /gene="nadB"
                     /locus_tag="PLO35_07195"
     CDS             4266..5864
                     /gene="nadB"
                     /locus_tag="PLO35_07195"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOH79491.1"
                     /translation="MKNYDFLVLGSGIAGLVFALQASQRGKVAVITKKSLFDCNTDYA
                     QGGIAAVLDVTDSFEQHCQDTYLAGAELGKKQVISKIIQEGPRLIQYLIDLGTDFTRR
                     DPSYDKRLENLSLTREGGHSHKRVAYAADSTGHQIMQALINRCHENTDIDIFEHHLAI
                     DLITQHHIVQDEGFIPGISCWGAYVMDLETNEVQIFRARKTMLATGGAAQIYAHNTNP
                     DVATGDGMAMARLAGARLANMEFVQFHPTAFWTPAGDTFLITEALRGEGAILRLQDGT
                     SFMESYHAQGNLAPRDVVSRAIDAELKRRGEKFCWLDATSIPPEKLKAHFPYIDSRLR
                     EYGIDFCQDPIPVAPAAHYFCGGVLSTIDGITDIHNLFAAGEVACSGLHGANRLASNS
                     LLEALVIAWHAANHPSLDACVNFPSIPTWKEMNMFNENEWVVISHNREIIGTIMHGYV
                     GIRRSRRLLKYALSRIENIYNEVNNFYQHNSVRKEVVETRNMAIIAIAVIRSALSRKE
                     SRGAHFLIDNPERDDARYQTDTII"
     gene            5883..6497
                     /gene="tmk"
                     /locus_tag="PLO35_07200"
     CDS             5883..6497
                     /gene="tmk"
                     /locus_tag="PLO35_07200"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOH79492.1"
                     /translation="MKGLFITFEGTEGSGKSTQVRMLEAVLRQMDIPCLKTREPGGPP
                     ISEAIRAILLNPAFTEMQAETELLLYMASRAQHTAQWIIPALERGEIVLCDRYNDSTI
                     AYQGAARDLDQDFIAALAKFATRSRVPDITFLIDIPVETGLARIQSRELDRMENEAIG
                     FHRKVREQYLRLASSQAFRYIVLDGTLDPNAIHTLILNHVLSRI"
     gene            6507..8108
                     /locus_tag="PLO35_07205"
     CDS             6507..8108
                     /locus_tag="PLO35_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH79493.1"
                     /translation="MTHSTHKTAIITIVCVWILAGIMLTGATSAFAQTQNKGSDIYTQ
                     LSTFTEVLQKVKQYYVTELDDEELIKAAIIGMLGNTDPHTSYFTKDEFSDFTTSTRGA
                     FGGLGIQIDKIGDYVTVVSPIEGTPAYRMGITAGDRIVKVDGTNIVGQSTDEAIKLMR
                     GDVGTQVVITISRPGVAEPLDFTIIRETIKIKSVPYFFKLDNGVGYIRITQFNENTTQ
                     ELRDALSSLETEGIRGLVLDLRSNPGGLLEQAVDTVNEFIGKDKLVVETKGRVANSNR
                     QHYTRFATRVRNYPIVVLVNEASASASEIFAGSLQDWDKGLVMGKTTFGKGSVQQLFP
                     LANGNGIKVTTSYYYIKSGRCIHKMLNDKLLQGKEVSDAEKQEEEKKTHELLYYTVNK
                     RVVYGGGGITPDIEAESDLLTNFAVELRRKNAFFNFAVDYMVQHEHVIPKDIRVDDSL
                     FSSFLTYVRQNDISYTAADLDSTASYIRSTLRSELIRKSHGDNEAYKATIDQDKQLQQ
                     AIALFDRFSTMEAMFEHAASIPKKR"
     gene            8130..8468
                     /locus_tag="PLO35_07210"
     CDS             8130..8468
                     /locus_tag="PLO35_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79494.1"
                     /translation="MKKLSLLIITMILVAALSAHPASSVSISYDARNSQITVDFEHSV
                     KSASDHYIQTLTLKINNKTIISQVYSLQEKSAGGLAIYRIPGLRKGDVIEVITDCNKG
                     GKKSGKLTLN"
BASE COUNT         2250 a         2473 c         2252 g         1865 t
ORIGIN      
        1 gtgtttgtgg aacagactga acagctcggg accggccatg ccgtcatgtg cactgcagac
       61 gcgctcaaag atgatgccgg agacgtgcta atcctctgtg gcgacgtacc tttgctcagt
      121 aaggaaaccc ttgaccggct ggtgaaccat catcgggaat ccagtgctgc ctgcacggtt
      181 ttgacagcct ttttggacga tcccggcagg tatggacgga tcctgcgtga cgagcagggt
      241 atggtgtccg ggattgtgga atttaaggac gccacggatg cccagcgtgc catcaaggaa
      301 tggaacaccg gcatctattg cttccgaacc agtgatatgt ttcaggccct cggcaagata
      361 tcaaaccaga accagcaggg tgagtattac cttacggaca ctttggccat cctgcgttcg
      421 gaggggaaga ccgtggcctc cattccactt gagcgccata tcgaggtttc gggggtgaat
      481 tcccagcagc agctggccga gctggaagac gccttcatag cttccatcag ggagcactgg
      541 ctcaaccagg gcgtggtaat gcacaacccc gccagcattt acatcgacga tgacgtgatt
      601 atcgagccgg acgtggagat cggccctcac tgtgtgatcc agggcaagac gaccctcgaa
      661 accggtgtcg tgatcggtcc ctcctgctta atcgacagct gccgcgtctg caacgacagc
      721 atcctggaag gccacaatat cctctcctat tcccatattc cagagcagca tatcgtcacc
      781 ttcggcgaga aagtgatcga agaagaagaa tatgagtgag cgctacctct actccccctg
      841 gcggattgac tatatcctgg gcgaaaagcc tgaggactgc gtgctttgcc gtttcaagca
      901 agagggggag gacaaggaca atctgatcat ctggcgcgga aagctggcct acgtgatgct
      961 gaaccgctat ccttacaaca atggccatct gatgatagtg ccttatgagc acaaaagctg
     1021 tctaaccgac ctcgatgtag ctaccctgca ggaacttgca cggctcagcc aggttaccga
     1081 actggtattc aaagagcagt tccactgtga aggcatcaac ttgggcttaa atcttggccg
     1141 ggccgccgga gcgggaatag aagagcattt gcacatccat atggtgccca ggtggatggg
     1201 tgacgacaac tttatgagcg tgatcggagg ccggagagtg atcccggaag attttaccca
     1261 aagctggtca cggcttcgtt ccgcgtttgc caaccacatg atggacaagg gataatgatc
     1321 caggatttga cagaaaaccg ggatcccaaa aagctgcgct caaacagccg cgtctggtat
     1381 atagtgatcg ccgtggctac cttgctggca gcaggttacg tcatctaccg caactatttg
     1441 catgatgaca atcccctgcc gcctggcgaa gaaagcatgc cagccatcaa agtaatcgtg
     1501 agtaatggct gtgggtatga aagcctggct accgattatg ccaattatat cagcaaactc
     1561 aacgtcgagg tggtcaaact gacagacacg cccaaaccta tctatgacaa atccctgatc
     1621 gtggtcagga aacaggatga gcaggacctc aaacgcctgc aggccatgac tggcatccag
     1681 cgtttcactc tggctgtcaa cgaagcctcc gaagccccgt tcatcatcat tctgggccgc
     1741 gattatcagc agtttatgaa agagaaccaa ggaggaacct ttggcagata acaccaagct
     1801 aaacgccatt ttgggatggc tgaacgagaa gaaagcggaa aacatcagcg tctatgacgt
     1861 tcagaagagc agcggttaca ccgatgtcat catcgtgtgc gaaggggccg ccgatctgca
     1921 taaccaggcg atagccaatc atttgttaga tatggccaaa gctaaccact ttcacgtgat
     1981 gagcaaggaa ggcatggagt ttggccgttg ggtgctgatc gatgtaggcg atgtggtggt
     2041 ccacattttc ctctccgata cccgctctta ctacgatatc gacaaactct tcaaaaaagt
     2101 gcaggaaacc gaggacccct ccgaatctat aagtaaggat acacccgcca gcgagcagac
     2161 aacatgataa aagacattat acgcgacaac atcattgagg tgctgaatgc ccacggctat
     2221 acccatgagc gggagtttgt ggttgaaatc cccaacaatc aggagtttgg ggattattcc
     2281 tccaacgccg ctctggtgct ctccaaagag aacaagacag ctcccaagct gatggcggaa
     2341 acgctgatca aggacctcaa gaaaaacaag ttcttcaaga aagtggagat ggccgggcct
     2401 ggattcatca atttccacct ctcacctgcc ctctatcaga tgatgctgtg gactatccac
     2461 ggcaagggag aggactacgc ggccactgat ctcggcaagg gcgaaaaggt cctgctggaa
     2521 tttgtgagtg ccaatcccac tggcccgctc aacatcgtta gcgccagggc cgccgccttt
     2581 ggagacactc tctaccggat catgaagttt gtgggctacg accccgcccg ggagttttat
     2641 atcaacgacg ccggcaatca ggtggacatc ctggccgaat ctctggaact gcgtctcaga
     2701 gagttgcagg gcgagcgcat tggtgatttc ccctatgagg cttatcacgg tgaatacgtc
     2761 aagcacctgg cccagaggct caatgccgtg gaaggcgtca gagtgtttat gatgacggaa
     2821 aaagaccgca tggaccacct caaggagttt gccctctccg agcttctgga gatgcagcgc
     2881 ctcagtctgg aaaagtttgg cgtggtcttc gatggctggg tgtcggaaaa gaccctgcgc
     2941 gccgaaggcg tggtggaaga agtgctcagc tatcttacgg aagcggactg cacctatgaa
     3001 aaggaagacg ccatctggtt tcgttccacc gattttggag acgacaaaga ccgcgtgctg
     3061 atgaagagcg acggctccat cacctatttc gtgccagatc tggcctacca tctcaccaag
     3121 attcagaggg gctacagcac tttgatcgat atcttcggtc cggaccatca cggctacgtt
     3181 ccacgtctca aagccgcctt caaagctctc aaatatgacg aagagatgtt ggagattatc
     3241 ttcctgcaac aggtcaacct cttcgagagc ggtgaacgcg tcaaaatgag caaacgggcc
     3301 ggcaaaatcg tgaccatgga cgatcttgtg agcgtggtgg gcaaagacgc ggcccgttat
     3361 tttttcattg cccgcaaagc caacgcccat ctcaatttcg acctcgaact ggccctcaaa
     3421 caaaacaacg aaaaccccgt ttactactgt cagtacgccc acgcccgcat ctgcagcatc
     3481 gtcaagaaag ccaaaaaaga ccgcgtcctc cccaagacat ataaaaaaga gctggccctc
     3541 aagctcaata aacctgagga actggccctg atccgcaaat tgaccgacct gccggaattg
     3601 ctgcagctga tctccatcca tcgcgagcca catcgtctgg ccacctgggc tgaagagctc
     3661 tgcgggctgt tccaccgcta ttacaacaaa tatcaggtgg taagcgccaa gaataaagaa
     3721 ctctctcaag cccgtctcct gctgatagat accgtccgca tgagtctggc cctggccttt
     3781 gacctgatgg gcatcagcgc tcccgaaaag atgtagggat tgggattact gatggctgaa
     3841 tttcgccgga tgcgaatgga agacctgccc agaatctatg agattgagcg gctggccttc
     3901 tgtgatccct ggccggatga agccttcagc atgatgaatg gctatcacag ctatgtggca
     3961 ttgagagaag gacttctgat cggctacatc atctgcctca gcgctctcga cgaaagctcc
     4021 atcgccaatc tggccataga cccccagttt caaagacagg gtttcggtaa agaactgcta
     4081 agattcgcca tggacgagct cgccacccag ggcatcaaga ggatgtttct ggacgtgcgg
     4141 cgcggcaacc tggccgctca gggactttat gagcagtttg gctttgtgca tgtaggagtc
     4201 agaaagggct actacagcga ccctcctgag gacgctctgg taatgatgtg gaccaaggta
     4261 actgaatgaa aaactacgac ttcctggtgt tgggctccgg tatcgctggc ctcgtgttcg
     4321 cgctgcaggc ctcccaacgt ggcaaagtag ccgtcatcac caaaaagagc ctcttcgatt
     4381 gcaacaccga ttacgctcag ggaggcatcg cggccgtcct ggacgttacg gattcctttg
     4441 agcagcattg tcaggacacc tatctggccg gcgcggagct gggcaaaaaa caggtgatca
     4501 gcaagatcat tcaggaaggc ccacgcctca ttcaatacct gattgacctg ggcaccgatt
     4561 tcacccgtcg cgatcccagc tacgacaagc gtctggaaaa tctttccctc acccgcgaag
     4621 gtggtcacag tcacaaacga gtggcctacg cggctgattc taccgggcat cagatcatgc
     4681 aggcacttat caatcgctgc catgaaaaca ccgacataga tatctttgag catcatctgg
     4741 ccatcgacct catcactcag catcacatcg ttcaggacga gggtttcatc ccgggcattt
     4801 catgctgggg agcttatgtg atggacctgg agaccaacga agtgcagatt ttcagggcca
     4861 gaaagactat gcttgccaca ggtggcgcgg cccagattta tgcccacaac accaatcccg
     4921 atgtggccac cggagacgga atggccatgg cccgtctggc tggagcgaga ctggccaaca
     4981 tggaatttgt ccagtttcat cccaccgcgt tctggactcc cgcgggagac accttcctga
     5041 tcactgaggc cctcagaggc gaaggcgcca tcctcagact ccaggacggc accagtttca
     5101 tggaaagcta tcacgctcag ggcaatctgg ctccccgcga cgtggtttcc agggccatcg
     5161 acgcggaact gaaacgccgc ggtgaaaagt tttgctggct ggacgccact tccatcccgc
     5221 ctgaaaagct caaagcccac ttcccttaca tcgattcccg cctcagggag tatggcatag
     5281 atttttgcca ggaccccatt ccagtggccc ccgcggccca ttatttctgc ggcggagtgc
     5341 tatccaccat cgacggtatc accgatatcc acaatctctt cgccgccgga gaagtggcct
     5401 gcagtggtct gcacggggct aatcgtctgg cctccaattc gttgctggaa gcacttgtga
     5461 tcgcctggca tgccgccaat catccttctt tggacgcatg cgtcaacttc ccctccatcc
     5521 ccacctggaa agagatgaac atgttcaacg aaaacgagtg ggtggtgatt tcgcacaacc
     5581 gtgagattat cggcaccatc atgcacggct acgtggggat ccgtcgttcc cgtcgcctgc
     5641 tcaaatacgc tctctccaga atcgaaaaca tctacaacga agtcaacaac ttttatcagc
     5701 acaactccgt ccgcaaggaa gtggttgaga cccgcaatat ggccatcatc gccattgccg
     5761 tgatccgcag cgcgctatcc agaaaggaga gccgcggggc ccatttcctg atcgacaatc
     5821 cggagcggga cgatgcccgt taccagactg atacgattat ttagcttgag aaggttgaca
     5881 gcatgaaagg cctgtttatc acttttgaag gcaccgaggg cagcggtaaa agcacccagg
     5941 tcaggatgct ggaagccgtt ctgagacaaa tggatattcc ctgcctgaaa acccgcgaac
     6001 cgggaggtcc acccatcagc gaagctatcc gcgccattct tttgaaccca gcttttaccg
     6061 aaatgcaggc tgagacggag ctcttgctct acatggccag ccgcgcgcaa cacacagccc
     6121 aatggattat tccagccctg gaacgcggag agatagtgct ctgcgaccgc tacaacgatt
     6181 ccaccattgc ctatcagggc gcggcccggg atctggacca ggattttatc gccgccctgg
     6241 caaagtttgc cacccgatcc agagttccgg acatcacctt cctgatagat attcctgtgg
     6301 agaccggcct cgccagaatt caatcaaggg agttggaccg catggaaaat gaagccatcg
     6361 gctttcaccg caaggtgcgt gaacaatatc tgcgccttgc ttcatcacaa gctttcagat
     6421 atattgtctt ggacggcacc ctggatccca acgcgatcca cacgctgatc ctcaaccacg
     6481 ttcttagccg catttaggag tatttagtga cgcattcaac ccacaagacc gctatcatca
     6541 ccatcgtctg tgtatggatc ctggcgggga tcatgctgac gggtgccacc agtgcctttg
     6601 cccagaccca aaacaagggc tcagacatct acactcagct cagcaccttc accgaggtcc
     6661 tgcagaaagt aaaacagtat tatgtgaccg agctcgacga cgaagagctc atcaaagcag
     6721 ccatcatcgg catgctgggc aacacggatc cccacaccag ctattttacc aaggatgaat
     6781 tctcagactt taccacctcg acccgcggag cctttggtgg cttgggcatc cagatcgaca
     6841 agatcggaga ttacgtcacg gtggtctccc ccatcgaagg tacgcccgct taccggatgg
     6901 gaatcacagc tggagaccgg attgtgaagg tggacggcac aaacatcgtg ggccagagca
     6961 ccgatgaagc catcaaactg atgcgcggtg acgtcggtac ccaggtggtg atcacgattt
     7021 cccgtcccgg tgtggctgaa ccgctcgatt ttaccatcat ccgggaaacc atcaagatca
     7081 aaagcgtccc ttatttcttc aagctcgata atggagtggg ctatatccgg atcacccagt
     7141 tcaacgaaaa caccacccag gagctccgtg acgctttgag cagccttgaa actgagggga
     7201 tccggggact ggtcctggac ctgaggtcca atcccggtgg cctcttggaa caagccgtcg
     7261 atacagtcaa cgagtttatc ggcaaagaca aactggtggt ggagaccaag ggccgcgtcg
     7321 ccaattccaa ccggcaacac tacacccgtt ttgccacacg ggtgcgtaat tatcccatcg
     7381 tggtgctggt gaatgaagct tccgccagcg cttccgagat tttcgcgggc tcgttgcagg
     7441 attgggacaa aggtctcgtg atgggcaaaa ccacctttgg caaaggcagt gtgcaacagc
     7501 tcttccccct cgccaatggc aacggcatca aggttaccac ttcctactac tatatcaaga
     7561 gtggccgctg catccataag atgctgaacg acaagcttct gcaaggcaag gaagtgagcg
     7621 acgccgaaaa gcaggaagaa gagaagaaaa cccacgaact gctctactac accgtcaata
     7681 aacgcgtcgt ctatggcggc ggaggcatta ccccggatat cgaagccgaa agcgacctgc
     7741 tcaccaattt cgccgtggaa ctgcgccgta aaaacgcctt cttcaacttc gcagtggact
     7801 atatggtgca gcacgaacac gtgatcccaa aagacatccg ggtggatgat tccctcttca
     7861 gcagcttcct cacctacgtc cgtcaaaatg atatcagcta caccgccgcg gacctggaca
     7921 gcaccgcttc ctacatccgc agcactctca gaagcgaact gatccgcaaa tcccacggcg
     7981 acaacgaagc ctataaagcc accatagatc aggacaaaca acttcaacag gccatcgcgc
     8041 tctttgaccg cttcagcacc atggaagcca tgtttgagca cgcagcctca atccccaaga
     8101 aacgttaagc acccaccaga ggagtaacca tgaaaaaact cagcctcttg atcatcacca
     8161 tgatccttgt cgctgccctc agcgctcatc ccgccagcag tgtcagcatc agctacgacg
     8221 ccagaaacag ccagataacg gtagattttg agcacagtgt caaatccgcc tccgatcatt
     8281 atattcagac cctcacgctc aagatcaaca acaagaccat catcagccag gtctattcct
     8341 tgcaggaaaa atccgccggc ggattggcca tttatcgcat ccccggcctc aggaaaggcg
     8401 acgtgatcga agtgatcaca gactgcaaca agggtggtaa aaagagcgga aagctgacgc
     8461 tcaactaggc atatctaccc atctgtcagc ctgagttatt tctcaccttc aggctgtaaa
     8521 catctaagac acaaaatctt attggccgtt tgatgctcat gccagccgtg atgtcatgcg
     8581 gcctttattc tgttaaaaaa cggttttaca attcattccc cttagcctcc tttacatcac
     8641 ctttacatca cctttacctc tcctatgcct tggcatggga ggtgtaaggg atacgtgtat
     8701 gagatgagaa caatatgact gatgaataat ggtatcactg gcttcagagc ttgtgaaaaa
     8761 actcgcgaaa cctgagactg acatagaccc gttggagttc aagcggccac gataagagta
     8821 tatatggcag cgagtttgcg
//
LOCUS       DAOSFN010000105         8835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6751_length_8835_cov_8.933713, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000105 DAOSFN010000000
VERSION     DAOSFN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8835
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6751_length_8835_cov_8.933713"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLO35_07215"
     CDS             complement(<1..626)
                     /locus_tag="PLO35_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOH79495.1"
                     /translation="MIQEYRFKGYGPQGKLVQGTFSVGSSKEAKAYIAQLADKYQLRV
                     ESVERKRDYLYKVSIPGKKPFQGRQSAFSKDEVASALTKMGYQKYTINPVLFDLPIKP
                     SNADIMMFIKLSATMLRDKMSFGKILEMLAEEQTNRAMKDALGQIETQLKAGAEGKEV
                     FNRFGHIFGKFPAYMLGLATRSGNMAEVFDATSKFIERDMEIRKSIKST"
     gene            complement(861..1361)
                     /locus_tag="PLO35_07220"
     CDS             complement(861..1361)
                     /locus_tag="PLO35_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79496.1"
                     /translation="MAPYYRNHTIPKPHSKKKGDYHGNPTNSFDRTQRDHRRRGYRRR
                     YRNVQPSGLQLQKTAIASDAQQYAAEVVQHYKTPVSQGGAMDTSGTLAASMTEAAVAA
                     KIGIGAAAGSAVANENGRYYVSDADTTEVEITGIGKAVRGGKSPKIVTTITFPNGDIR
                     AAATDE"
     gene            1798..3942
                     /locus_tag="PLO35_07225"
     CDS             1798..3942
                     /locus_tag="PLO35_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79497.1"
                     /translation="MSSKLSAILILALAISGLVAQYPYWTNYYNTSRIKEFCMDDDYF
                     WCLSYSPFKVNRATGQVTRLSHADYGLPGGLIDDIDIDSEGNIWMLPSPGGLLKWDGQ
                     TQTLYSAEYMTNYCRDLVISSPELIWFTKYSGMVVEFDGTGFSYLGGGFENSNPRLDN
                     NGDLWFWADHAIDLNFYHSLVHYDGSEFHYTWVAPENNLDYLYAAFDVNNQPWAGTRQ
                     NGVGKWINNQWVMYKTNNSGLLHNEVRCLEFDSANTLWMGTPAGISTLNGSTWGSYTP
                     ANSALGNRIPNRVLVEDGNRIWFATDNGLVKIENGELSVIDTSLDGIPFSIAFEQVEA
                     PNGEMWVAFGSGLYKFDGCSWIAPSLPVPEFEVTGVAVDRLGVVWVSGNAGLLRWEGG
                     VWTHYNSQNSGLPSNVCNSTAVDSQRRVWVATNAGLACFDGQSWQSWNSSNAPFPDSE
                     VGVLKIDPQGRIWVTNNDFSDYYNEWGWGAAAVWDGLAWSYVDVPYYGDGTEFILDMD
                     FYGNRACFTTLGGLIFLEDELWRFDNLSSASPNLYGNIVCSSYDAVGNLWLAANGLGR
                     YDGTSWSAYRSSNSGLPTNQCYNVFIDSSGLVWVSTDSSGMNVFNYPASVANADPVVP
                     VINTLKAWPNPFRQDISIEMELTRSSAQEISVYNLRGQKVRGLALGISNDGKGLASWD
                     GRDNDGADCPAGIYLIRAGRGAGARAVKVLKL"
     gene            complement(4213..4935)
                     /locus_tag="PLO35_07230"
     CDS             complement(4213..4935)
                     /locus_tag="PLO35_07230"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HOH79498.1"
                     /translation="MSKLLLFCAMLLATTLVFDCEAKTTVPSGPRLQKIQKSISPRLA
                     EELSTLDLELGSPIYIRIFKEELELELWVREDEAFTLFRTYPVNYYGGEGLGPKLAEG
                     DHRAPEGFYSVKPAQMNPYSHYHLAFNLGFPNAWDKAHKRTGSNIMVHGDTLSVGCFA
                     MTDSLIEEIYVLADAALRKGQPAFDVHVFPFRMTQENMDRFRNSEHIAFWQELRPGYD
                     LFEARGFIPPKVKIEKGVYKFE"
     gene            complement(5030..>5710)
                     /locus_tag="PLO35_07235"
     CDS             complement(5030..>5710)
                     /locus_tag="PLO35_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79499.1"
                     /translation="GQPLKIWMANTETADLSAGWLEPSRYSLVFDGSIDFPLGVNTII
                     IPLDNPFLFTGANLALRCHRPMDSVYYSNSNHFFYNNDTAFPNRSRYIQSDSTVYDPL
                     APSGTGTLSSNIPLTAFVVDQAEPLSLAAPQISIQPQVNGMMLSWPVLPGYYGYRVFA
                     SPDPLQWPDEPLLTVYEPQVLIIPAVSRSFYRVMAFSYTHDLRAGLDKLRSETTRRDE
                     GINDTQKP"
     assembly_gap    5711..5810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5811..8620)
                     /locus_tag="PLO35_07240"
     CDS             complement(<5811..8620)
                     /locus_tag="PLO35_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOH79500.1"
                     /translation="MIATSYFKRLSLMTLLIMILGLGQASYVNKLPMRVVQPDGSELD
                     LLASGDEYHNWLHDESGYTIIPSDTGWYCYAEPDGEAVKPSELIVGQADPAAHGLKPG
                     ANISPRLYKERRKTAFWMPEGRDAPTTGTINNLVIYIRFSDEAEFGQSNSIYDGWFNT
                     STNSQKNYYLEASYNQLTVNTHFYPAPVNGYVVSWQDSHPRSYFQPYSTSNPGGYNGD
                     DQRRDREFTLLQNACAAVGPSIPASLTIDSDNDGRVDNVVFIVKGAAGAWSSLLWPHR
                     WSLYDRYVYINGKRVYDFNFQLQTFLASRGVGVICHEFFHTLGAPDLYHYTDNGIDPA
                     GSWDIMETDKNPPQHMTAFMKWKYGDWIPSIPVITADQAYTLNPITSSTGQCYRINSN
                     HPNQYYVVEFRKKTGTFESSIPNSGLLVYRIDTSCGNGNADGPPDELYIYRPGGTTTS
                     NGTLSSANFSSETGRVAIYSGTNPSPFLQDGSAGNLYLCEIGSSSGTTMSFRKGMPWI
                     NFEPNPYVQNFDVTVFPPDGWTSVAQSGSYTFSRVTSGTNPNCSPQYGAGFIKFNSDT
                     SPAGSSALLVSPRVVVSDPDDYAYQVSFWMYRDGNQSTALDKVEIYLNTSDSLSGSPT
                     LLGTIHRARQQSPVESAPGWKQYFFSFPISGTGDHYAILRAISAGGYNIYVDSFRLGK
                     VPFPATLTAPAQGSTVANWSAGLSWQSGGGGPTGYQLWLGTNNPPSNIVNGQDLGSLT
                     SYLPANLSFDTTYYWKVLPYGPGGSANDCPVWSFTHVDARIGVLPHTQNFDGVLFPDL
                     PLGWTDVVNSSSTGASIRTNTGSSVSQPNSVYFYNSTDASADLRLVSPQLLVPIGQCR
                     ISFYARSGSNGYPLLLGTQVSPDGIFEPLSTITLTNTHTLYELTLASYSGTNAYLVFK
                     HGMGGTYRGIYLDDLSIEPQAQT"
BASE COUNT         1948 a         2218 c         2491 g         2078 t
ORIGIN      
        1 gtggacttga tgctcttgcg gatttccata tcccgctcga taaacttgct ggtggcgtca
       61 aacacctcgg ccatattacc gctgcgggtg gccaaaccca gcatgtaggc gggaaacttg
      121 ccaaagatat ggccaaaacg gttaaaaact tcttttcctt cagcccctgc tttaagctga
      181 gtctctatct ggcccagggc gtctttcatg gcccggttgg tctgctcctc ggccagcatt
      241 tccaggatct tgccaaagct catcttgtca cgcagcatgg tggcgctgag cttgataaac
      301 atcatgatgt cggcattgga aggtttgatg ggcagatcaa agagaaccgg gttgattgtg
      361 tacttctgat atcccatctt ggtgagggcg gatgccacct cgtctttgga gaaggccgat
      421 tgccggccct gaaatggctt tttgcccgga atactgacct tgtaaaggta atccctctta
      481 cgctccacag actctacccg gagctgatat ttatctgcca gttgggctat gtaagccttg
      541 gcctccttgg aagaacccac cgaaaaggtg ccttgcacca gcttgccctg agggccgtag
      601 cccttgaaac ggtattcctg tatcactggc aatccttatc tatactgaag agcatgaatg
      661 aaacgcgctg ccaggaaaaa tactaatcca gcgtctgaac actaaaacat tgaaaacaca
      721 tcctaaacca gttgccatcc ggcttgggag cttggggtca tctaacatac gcagagccgg
      781 tggtgttatc catccggccc tgctcatgaa tagtgtacca ggctttgtga tggtgttata
      841 ccacctggta taagttcttt ttattcgtcg gttgcggcag ctctgatgtc accattgggg
      901 aatgtaatgg ttgtcacaat cttggggctc ttaccaccac gaacagcttt tccaatgcct
      961 gtaatctcaa cttctgttgt atcggcatca gatacataat acctgccgtt ttcattggca
     1021 actgcagatc ccgcagcagc accgataccg atcttggcag ctacggcagc ctcagtcatg
     1081 ctagcagcga gggttccgga agtgtccatg gctccgcctt gggaaacggg ggtcttatag
     1141 tgctgaacga cttccgcagc atattgctgc gcgtcagaag caatagcggt tttttggagt
     1201 tgtaagcctg acggttgaac attccgatac cgacgacgat agccacgccg acgatgatca
     1261 cgctgagtac gatcaaaaga atttgttggg ttcccatggt aatctccttt ttttttggaa
     1321 tggggtttag gtatcgtatg attgcgataa taaggggcca taaaaattct caagatatat
     1381 ttggtcagaa cttttccaca gactgtctct atttttccac aagatggatg cttctatccc
     1441 taacatgctg tattaatgat atatataaat tccatactgc gtccttttga atccacacag
     1501 cgcgtaattt cttccacaac gctccctccc acgagagcaa agactcgttt tccggggtaa
     1561 aaacaagtct ctgattaggg cctttagcct gcaccttttt accacgtact tcgggcagat
     1621 ctgctgaacg ggcctaacca ggtctctggt gtcggatgaa gtggttgcac cctcaggccg
     1681 tgctggtttc aaggtccgcg gcgccatgca agcctccctg ccactgctgg ataaatgcct
     1741 gatccggaac attttttgca tatccatttc gtagtctaac acaagaaggg agcagccatg
     1801 agcagcaaat tgagcgctat cctgatcctg gccctggcca tcagcggtct ggtggcccaa
     1861 tatccatatt ggacaaacta ttacaacact tccaggatca aagaattttg tatggacgac
     1921 gactatttct ggtgcctcag ctactctccc ttcaaggtga atcgcgccac ggggcaggtc
     1981 acgcgtctca gccacgccga ttatggcttg ccgggcggtc tgatcgacga catagatatt
     2041 gactctgagg gcaacatctg gatgctgccc agtcccgggg ggttgttgaa atgggacggg
     2101 caaacccaga ccctctacag cgcggaatac atgacgaatt attgccggga cctggtgatc
     2161 tcgagcccgg aactgatctg gttcaccaaa tacagtggta tggtggtaga gtttgacggc
     2221 acgggtttca gctatttggg aggaggcttc gaaaacagca atccccggct ggacaacaac
     2281 ggcgatctct ggttttgggc cgatcacgcg atagacctga acttttatca ttccctggtc
     2341 cactacgacg gttccgagtt tcattacacc tgggtggccc cggagaacaa tctggactat
     2401 ctctatgccg cttttgacgt gaacaaccag ccctgggccg gaaccaggca aaacggcgtg
     2461 ggcaaatgga taaacaatca gtgggtgatg tataaaacca acaattccgg cctcctgcac
     2521 aacgaggtca gatgcctgga gtttgactcc gccaatacac tctggatggg tactccggct
     2581 gggataagca ctttgaatgg tagcacctgg ggcagctata cgccggcaaa ctcggcactt
     2641 ggcaatcgaa tccccaacag agtattggtc gaagatggca accggatctg gttcgctacc
     2701 gacaacggac tggtgaaaat cgaaaacggc gagctcagtg tgatcgatac ctccctggac
     2761 ggcattccct tctcgatcgc tttcgagcag gtcgaagctc ccaatggcga gatgtgggtc
     2821 gcttttggca gcgggctcta taagtttgac ggctgcagtt ggatcgcccc aagcctgccg
     2881 gtcccggagt ttgaagtcac gggagtggcg gtagatcgcc tgggagtggt ctgggtgagt
     2941 ggtaacgctg gtctattgcg ctgggaggga ggggtttgga cccattacaa cagccagaat
     3001 tccggcctgc cttccaatgt ctgcaattcc acggcagtag attcccaaag acgcgtgtgg
     3061 gtggccacca atgcgggcct tgcctgcttt gacggccaga gctggcaaag ctggaatagc
     3121 tccaatgctc cctttccgga ttcggaagtg ggggtgctga agatcgatcc ccagggccgc
     3181 atctgggtca caaacaacga ctttagcgat tactacaacg aatggggctg gggtgccgcg
     3241 gcggtttggg atggcctggc atggagctat gtggatgttc cgtattatgg agacggtacc
     3301 gagtttattc tggatatgga tttctatggc aatcgcgcct gttttaccac tctgggcggc
     3361 ctgatctttc tggaagacga actgtggcgc tttgacaacc tcagttccgc ctcccccaac
     3421 ctctatggca acatcgtttg cagctcctat gacgctgtgg gcaatctgtg gctggcggcc
     3481 aatgggttgg gtcgctatga cggcacatcc tggagcgctt atagatcctc caattccggc
     3541 ctgccaacca accaatgcta caacgtgttt atcgattcca gtggactggt ctgggtgagc
     3601 accgattcct ccgggatgaa tgtgttcaac tatcccgcct cagtggccaa tgccgatccg
     3661 gtggtcccgg tgataaatac cctgaaagcc tggcccaatc ccttccgcca ggatatcagc
     3721 attgagatgg aactgacgcg ctcatcagct caggagataa gtgtgtataa cctgcgcggc
     3781 cagaaggtgc gcggccttgc cctcggcatt tccaatgatg gtaaaggctt agccagctgg
     3841 gacggccgcg ataacgacgg agcggactgc ccggccggga tttatctgat cagagcgggc
     3901 agaggagctg gggccagggc cgtaaaggtg ttgaaattgt agttttgggt ggcacagctc
     3961 cagaccggaa gaatcggcgt agaggtattg tgaatgattg ctccagagtg gatgcacttc
     4021 tgaattatgg tcagctaacg cgccagccga ggaggggcaa aggtctcttt tgccgtggcc
     4081 cgcagggaca ctcagcgcgg cctctaatca gataaagcac tcttttactg gtctggcagg
     4141 ccatggcaca ggcgctgatc gcgcctcagc aaacgaggac ctttgctgtt ccataagctg
     4201 agagaggcta atctactcaa acttatagac tcccttttca atcttcacct taggagggat
     4261 aaaacccctg gcttcaaaga gatcatagcc tggccgcaac tcttgccaga aggcaatgtg
     4321 ttcgctgttc cgaaagcggt ccatattctc ctgagtcata cgaaagggaa agacatgcac
     4381 atcgaaggct ggctggccct ttctgagagc cgcatcggcc aggacgtaga tctcctcgat
     4441 gaggctgtcc gtcatggcaa aacagcccac ggaaagggtg tcgccatgca ccatgatatt
     4501 gctgccggtc cgcttgtggg ccttatccca ggcgttgggg aagcccaggt tgaaggcgag
     4561 gtggtagtgg ctataaggat tcatctgggc tggtttgacg ctgtaaaagc cttccggggc
     4621 gcggtgatct ccttcggcca gtttgggtcc caggccttct ccaccgtagt aattgacggg
     4681 ataagtcctg aagagggtga aggcttcatc ctcccgcacc cagagctcca gttccagttc
     4741 ctctttaaat atcctgatat agatggggct gcccaactcc aagtccaggg tgctcagttc
     4801 ctcagccaac ctggggctga tgcttttttg aatcttttgc aggcgcggac ctgagggcac
     4861 agtggtcttg gcctcgcagt caaagaccaa agtggtggcc agcagcattg cacagaataa
     4921 caggagtttg ctcatccttt ctcccaagtg acgtgtttac cataagctgg agtgagttca
     4981 tgtccagtgg gaatcacctg cctgttcagg atccttgggc tggaatatat caaggttttt
     5041 gagtatcatt aataccctca tccctcctgg tcgtttcgct tctcaacttg tccagccctg
     5101 ctcgcagatc atgggtgtag ctgaacgcca tcacccgata aaaagacctt gagacagcag
     5161 gaatgatcag cacctggggc tcatagacgg tcagcagagg ttcatccggc cactgcagag
     5221 gatcagggct cgcaaagacg cgatagccat aatagccagg cagcacaggc cacgacagca
     5281 tcattccgtt gacctgaggc tggatgctga tctgtggggc tgccaggctg agaggttccg
     5341 cctggtccac cacaaaagcg gtgagcggga tattgctgct cagggttccg gttccagatg
     5401 gcgcgagggg atcgtagacg gtgctgtcgc tctggatata gcggctgcgg tttggaaaag
     5461 cggtgtcgtt attgtaaaag aagtgattgc tgttgctgta ataaactgaa tccatgggac
     5521 ggtggcagcg cagggccaga ttggcacctg taaataggaa gggattgtcc agggggatga
     5581 tgatagtgtt tacccccaac gggaaatcga tgctgccatc gaagacgagg ctgtagcggg
     5641 aaggttccag ccagccggca ctcagatcgg ccgtttcggt attggccatc cagattttga
     5701 ggggctggcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtctgggcct
     5821 ggggctcgat gctcaggtcg tccagataga taccgcgata tgtacctccc atgccgtgct
     5881 tgaagacgag gtaagcgttt gtcccgctgt aggatgccaa agtcagttcg tagagggtgt
     5941 gggtattggt gagcgtgatg gtgctcagag gctcaaagat tccatccgga gacacttgcg
     6001 ttcccagcag cagaggatag ccgttggatc ccgagcgggc gtaaaagctg atccggcatt
     6061 gcccgatggg gaccaaaagc tggggactca ccagtctcag atcggctgag gcatcggtgc
     6121 tgttgtaaaa atatacgctg ttgggctggc tgactgacga gccggtattg gtcctgatac
     6181 tggccccggt ggaggagctg tttaccacat ccgtccagcc cagcggcaga tcaggaaaga
     6241 gcaccccatc aaagttttgc gtatgaggca ggacgcctat cctggcatcc acgtgggtga
     6301 agctccagac gggacagtcg ttggcagagc cacccggtcc gtagggcagc actttccagt
     6361 agtaggtggt gtcaaagcta aggttggcag gaagatagga tgtcaagctt cccaggtcct
     6421 ggccattaac gatattggag ggggggttat tggtgcccag ccagagttgg tagcccgtgg
     6481 gtcctccgcc tccgctttgc cagctgaggc cagcactcca gttggctacg gtgctgccct
     6541 gggctggagc cgtcagagta gctggaaagg gaaccttgcc cagccggaag ctgtccacgt
     6601 agatgttata acccccagcg cttatagccc tcaggatagc atagtgatct ccggttccgc
     6661 tgatagggaa gctgaagaaa tactgcttcc agccaggcgc gctctccacc ggactctgtt
     6721 gtctggcccg gtggatggta cccaataagg tgggagagcc actcagactg tcgctggtat
     6781 tcagatagat ttccaccttg tccagagccg tgctctggtt gccatcgcga tacatccaga
     6841 aggatacttg atacgcatag tcatcgggat cgctgaccac taccctggga gaaacgagca
     6901 gagcggaact gccggccgga gaagtgtcgc tgttaaactt gataaatccc gccccatatt
     6961 gaggactaca gttaggattg gtaccgctgg tgacacggga aaaggtgtag ctgccgcttt
     7021 gggctacgga agtccatccg tctggaggga agaccgttac gtcgaagttt tgcacataag
     7081 ggttgggctc gaagttgatc cagggcatgc ctttgcggaa gctcatcgtg gttccggagg
     7141 aggaaccgat ctcacagaga tagaggtttc ctgccgagcc gtcctgtaag aacgggctgg
     7201 gattggtgcc actgtagatg gctactcttc cggtttcgct actgaaattg gcagaactaa
     7261 gcgtgccgtt gctggtggtc gtgccacccg gccgataaat atacagctcg tccggagggc
     7321 cgtccgcgtt gccgtttccg caggaggtgt cgatgcgata gaccagcaag ccgctgtttg
     7381 gaatggagct ctcgaaggtg ccggtctttt tgcggaattc caccacatag tattggttgg
     7441 ggtggttgga attgatgcga tagcattgtc cggtggagga agtgatgggg ttcagggtat
     7501 aggcctgatc ggcagtgatg acagggatgc tggggatcca atcgccatat ttccacttca
     7561 tgaaggcggt catgtgctgg ggtggatttt tgtccgtctc cataatgtcc cagcttccag
     7621 ccgggtcgat gccgttatcg gtatagtgat agaggtccgg agcgcccaga gtgtggaaaa
     7681 actcatggca gatcaccccc actcctctgc tggccaggaa ggtctgaagc tggaagttga
     7741 agtcatacac ccgtttgccg ttgatataca catagcggtc atagagactc cagcgatggg
     7801 gccaaagcag gctgctccaa gctccggccg cgcctttgac gataaagacc acgttgtcca
     7861 cccggccgtc gttgtcggaa tcgatcgtga ggctggcagg gatggaaggt cccaccgcgg
     7921 cacaggcatt ctggagcagg gtaaactccc ggtcccgcct ctggtcatca ccgttgtagc
     7981 ctcccggatt ggaggtggaa tagggctgaa aatatgagcg gggatggctg tcctgccagg
     8041 agaccacata accattgacc ggcgccggat agaaatgcgt gttcaccgtg agctggttgt
     8101 aactggcctc caggtaatag tttttctggc tgttggtgct ggtattgaac cagccgtcat
     8161 agatgctgtt ggattggcca aactcggctt catccgaaaa gcggatatag atcacaaggt
     8221 tgttgatggt gccggtggtg ggagcgtctc taccctcagg catccagaaa gccgtcttgc
     8281 gccgctcttt gtagagccgg ggactgatat tggccccagg cttgaggcca tgggcagcag
     8341 gatctgcctg gcccacaatc agttcactgg gtttgaccgc ttcgccatcg ggttccgcgt
     8401 agcaatacca gcctgtgtct gaaggaatta tcgtgtaacc ggattcgtcg tgcagccaat
     8461 tgtgatattc atcaccgctg gccagcaaat ccaattcgct gccatccggt tgaaccaccc
     8521 tcatgggcag tttgttcacg taagaagcct ggcccaggcc aaggatcatg atcagcaatg
     8581 tcatcaggga caagcgctta aagtagctag tagcaatcac gcggactcct tgctaagggg
     8641 attttccttt tgaaaacagg ctcaggaaag cgggggttga cgtcaaccat tttctggaac
     8701 tgcccgcaaa atgcttcaat ctccagctgg agtggggcgg atcatattgg gctggtctgc
     8761 tatagagtat cacccaaaca tgcaactggg tatcacttgt cacagtggtc gtaagtgttt
     8821 ataggattgt gagat
//
LOCUS       DAOSFN010000106         8766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6828_length_8766_cov_8.171852, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000106 DAOSFN010000000
VERSION     DAOSFN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6828_length_8766_cov_8.171852"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PLO35_07245"
     CDS             complement(<1..952)
                     /locus_tag="PLO35_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79501.1"
                     /translation="MYGNMVCNATATTAQVGMRSDPTGTATNFATRTTTTDWSASTDG
                     AAATSTCTLSNTVYPANGTTFTWTPPAPLDSSYSIGSAGDFISFATALAFLNTNYPLQ
                     GIPEGGTIFNVAAGEVFPNTELLPAITATGAPNRPITFRKTGDGANPILQVQGTTATT
                     DAAIKLMGGDYFTFERIDIQNYPSLSSLEYGFQLAAQSYNPCSHNSISYCSITLSRSN
                     SATKAILSAGATSAGNSNNSYLNLGIADCNTGIYLYGNGTAAYHNDGELVSNCVLTDI
                     SGQGIEINYSTSVRVTLNQISMCANNNIFMNTSTPGNAGIT"
     gene            complement(1048..1524)
                     /locus_tag="PLO35_07250"
     CDS             complement(1048..1524)
                     /locus_tag="PLO35_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79502.1"
                     /translation="MKRLIAILAFASVCVFCFAQSLGNYTFSHGVSSYTPISSGTQLG
                     NESTSGQRFVDPAIPLGGTATTGPGFPIGFNFYFNGQEYDRVGICASGWISLGQSWEV
                     TALNMSANTTTPLGYTSAINPSWLVSRIAGLANNMSAQAGSSLRIETIGILPTGCW"
     gene            complement(1873..3213)
                     /locus_tag="PLO35_07255"
     CDS             complement(1873..3213)
                     /locus_tag="PLO35_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH79503.1"
                     /translation="MPGIDLKKNILISASYKVLTMALSFLTGWISTRFLGVELKGQYS
                     YLITISSFAWLVLDLGIHKTYPYLIRKEAEQRLQLFTWSLLQFLIEFTLIAAVGLAFM
                     PFFSQALKFDFTAPIVVLVAGAISLTKLQLHMQMYYLGRDLVKTNSHYQALNSFVMLI
                     MVAGGYLAFRNSDRLLYVLISYNLAMACAVGAYVHSELFSRFWKGFRPGYILRAYSMG
                     WRVFLSSLFITLLIRFDVVLIRRFLGFEDLGVYAVAANIVDMLQMAANLVGSLLLVKL
                     SDITEDLERWNLLKKVFLGFFLLLGAANLGFVIVGKPLLTLMYGRDFTPVYNVYLWLI
                     PASFGLSFGSLFNTYLWSKGFPLISVVLPLIALLLNIGLNWILIPVMGIKGAALATSI
                     AYLAWFLAILIYEHFHSGKRIIGYLWPRGKDYVELWTVLRKEARKLTGGHAKLG"
     gene            complement(3219..4466)
                     /gene="serS"
                     /locus_tag="PLO35_07260"
     CDS             complement(3219..4466)
                     /gene="serS"
                     /locus_tag="PLO35_07260"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOH79504.1"
                     /translation="MLDIRFIRANAELVKAAVANKNEKADIDAILVWDDKRRKLQFDF
                     DTLKARQNQVSTEIATLKRAKADASQLLEEMARVADEVKALNQELTEVSNRLEGMLLT
                     VPNIPAENVPVGRDESANRVVKSWGEKPSFEFEPADHLAIAEANQFLDLARGAKISGS
                     GFPVYKGKGAQLERALINFMLNYHIDHHGYEELNVPLLVTRQTMTGTGQLPKLENDMY
                     HVDEDDLFLIPTAEVPVTNLYAGEVLPLSSLPIKHVAYTACFRREAGSYGKDTKGLQR
                     LHQFNKVEMVRFTEPEKSDAALEEMLVDAEHILQSFGLHYRVLELCTGDLSFASARTY
                     DLEVWAPGTGKYLEVSSVSTFRDFQARRANIRYRGPDGKPAFVHTLNGSGLATPRLMI
                     ALLESYQTGDKGLRLRDILKVML"
     gene            complement(4690..5469)
                     /gene="tpiA"
                     /locus_tag="PLO35_07265"
     CDS             complement(4690..5469)
                     /gene="tpiA"
                     /locus_tag="PLO35_07265"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOH79505.1"
                     /translation="MRDIIVAGNWKMNKDAFTTAEFCTSLAHMLKGQFLAGVQVIVAP
                     PYPFLSIALSALADSLVQVCAQDVSPHQDGAYTGEVSATMLASLGTAHCIVGHSERRQ
                     YHHESDRLIRDKLLRLFELRITPLLCIGETIVEREADQTEGVLRAQLNGAFEEVTLTS
                     GRDIVIAYEPVWAIGTGKTATSLQAQEAHAFIREWLRDRFGTEIADSIHILYGGSMKP
                     ENISELIAQPDIDGGLIGGASLKIGDFEAMINTALRAGVNT"
     assembly_gap    5621..5720
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5850..8735
                     /locus_tag="PLO35_07270"
     CDS             5850..8735
                     /locus_tag="PLO35_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOH79506.1"
                     /translation="MPSRPISLLFLAVAVFLIIANSLSATQPFWSMEPSTRVIDRADS
                     LTGFDITKYTINLYVNDSTHYIEGVVDAEVTAEAPLSSIQYRLEGGSLSVTSVLVDNV
                     SVPFTHQNGIINIPVSFSTGQSFMTRVSYNGIPGNSPAPYNIGLKFTSTGIYTLSNPD
                     AGRFYWPSYDHPWDKALVDWNIRVRSDWLAAANGIRTGITDNGDGTRTHHWVCSFPVA
                     TYVMGFAAAAYIESNQTAGALPIQNFVLPNQLNNAQVDFANVPQMIAHFSELYGPYPF
                     EKYGHMVVNMSTYAAMEHQTMTTFGAQYLTGNQNYESIVAHELTHQWYGNYLTPITMR
                     EVWLKESFATYSEFLWAHKKSGWDSALQYLRNSIQQYYISWENSNGPHTIFDPVYNLM
                     FAPPTYEKSASVLHMLRLKMGNPAFFNFIRALLTTYPNGNLNTAEFVALAQQHSGQDL
                     TQFFQQWIYSPGIPDGRFCIFHNGNGMAKVYARSQSPTATQFWLDVPLKLTGSAVADS
                     VVISAGPTWTSNVFAIGLDDDPQDIQIDPNHWVLTRSFATIDAQLTACLAYSGAVSVN
                     WNSLGVDFPLAGYHVFRKTLPDGPLVQVTPSPQTGMSYTDISVVNGSSYEYYVCAVDP
                     DGYISLPSNRMQASPITFPFDLGFLVVDETRAGNGTAFSPTDAQVDDFYATALQGFNY
                     TEWDLDTQGNPPLSTLARYPLIFWHSDDFSEIHLAELQDLVGSYVLSGGKLILSGWKH
                     PSVFNDGFLSRFLPGITPNLQNSAVFVAAHSDTYPSLYPDPSKLAAPWNGMLPMTYTF
                     SGADEALYTAEIVDDGPGDGEAAALRIQAGGEMVLLGFPLYFMLPDGVSAFLQDLVPE
                     LYPLVESDDEVNVPAPLSLSLYPNPYRGKGSLSLGLKAASLISLDVYNLRGQKVLAIQ
                     DLVPKTQGSELLYEIPSSYIDKLSSGCYLIRAKTSKGILTRRISLIK"
BASE COUNT         1981 a         2425 c         2268 g         1992 t
ORIGIN      
        1 ctgtgatgcc ggcattgcct ggcgtgctgg tgttcatgaa gatgttgttg ttggcacaca
       61 tggatatctg gttcagggtg acccttactg aagtggaata gttgatttcg attccctgac
      121 cggagatatc cgtcaaaacg cagttggaaa ccagttcgcc gtcgttatga tatgctgctg
      181 tgccattccc atacaggtaa atgcccgtat tgcaatctgc tatgccgagg ttcagatagg
      241 agttgttgct gtttccggca gaggtagcac ccgcggaaag gatggctttt gtggcgctgt
      301 tgctgcgact gagagtaatg ctacagtagc tgatactatt gtggctacag gggttgtatg
      361 actgcgcggc caattgaaag ccatattcca gcgaactcag cgaaggatag ttctggatat
      421 cgatgcgctc aaaggtgaaa tagtcacccc ccatcagctt tatcgccgcg tccgtggtgg
      481 cagtagtacc ttgcacctgc aggatcgggt tcgctccgtc gccggtcttg cggaaggtga
      541 tgggacggtt gggagctccg gtagcagtga tggcaggcag caattccgta ttgggaaaca
      601 cttccccggc cgctacatta aatatggtgc cgccttccgg aatgccctgc aggggatagt
      661 ttgtattcaa aaaggcaagg gcagtggcaa agctgataaa atccccagcg ctgccaatag
      721 aataggagct gtcaagcggc gcgggcggag tccaggtaaa agtagtgccg ttggctggat
      781 acaccgtatt gctgagggtg caggtactgg tagcggcggc tccgtctgtg ctggcactcc
      841 agtcagttgt ggttgtgcgg gtggcgaaat tggtggcggt gccggtgggg tcgctcctca
      901 tgcccacctg ggcagtggtc gcggtggcgt tgcacaccat atttccatac acgaactgca
      961 ccttgttggt ggtttcttcc agcctgattt gaaagttgaa gctgtcgtcg gttccacttg
     1021 aggcctttta gtagttgttc cattgcacta ccaacacccg gtaggcagga tgccgatcgt
     1081 ctctatcctg aggctggaac ctgcctgagc ggacatatta ttcgccagtc cggcgatcct
     1141 gctcaccagc caactgggat tgattgctga cgtgtatccc agtggagtgg tcgtgtttgc
     1201 tgacatgttc aacgcagtta cttcccagga ttggcccaga ctaatccatc cgctggcaca
     1261 aatccccacc ctatcatatt cttgcccgtt gaaatagaaa ttgaagccga tcgggaagcc
     1321 cgggccggtg gtagccgtgc ctcccagtgg gatggcaggg tccacgaaac gctggcccga
     1381 ggtggattca ttgcccagtt gcgtaccaga tgatattggc gtatatgagc ttacgccatg
     1441 actaaatgtg taattaccca aagactgggc aaagcaaaat acacaaaccg atgcgaacgc
     1501 gaggatcgcg atcaagcgtt tcataacagg ctccttttac ttcaatctga cctggcagat
     1561 cggttaatag caatacaaat cagagccgcg cctgttattt cacaagcagc ggacagctca
     1621 acgggaatgc tccagcgtca ggggatatat gtcaaggaat atctcaggaa ttgtcaaatc
     1681 ctgccattag agcagagcag gctgatacag agtgttaact tactgaacgg gatgggataa
     1741 agagtcactc cagagcattt gacacccgga cttatcccgc gacgccaaca cgcgtttgtg
     1801 tatttttttc ctcaatcagc gccaacttat cggcaagccc tcagaccctg gttgagagcg
     1861 tcctcagctc aatcaaccta acttggcatg ccccccggtc agctttcgcg cttcttttct
     1921 caggacagtc cagagctcta catagtcttt ccctcggggc cacagatatc cgatgatacg
     1981 tttgccggaa tggaaatgct catagatcag gatagccaga aaccaggcca gataagcgat
     2041 actggtagcc agagcggccc ctttgatgcc catgacgggg atgagtatcc agttgagccc
     2101 gatattgagc aacagagcga tgagtggaag gaccacactg atcagcggaa agcccttgct
     2161 ccagagatag gtgttgaaca gcgagccaaa ggagagccca aagctggctg ggatcaacca
     2221 tagatagacg ttgtaaacgg gggtgaaatc gcgtccgtac atcaaggtta acagcggttt
     2281 gcccactatc acgaaaccca gattggcagc tcccaagagc aggaaaaagc ccaggaacac
     2341 tttcttgagc aggttccagc gctccaaatc ttctgtgatg tcggaaagct tcaccagcag
     2401 cagcgagccc accagattgg ctgccatctg cagcatatcc acgatattag ccgcgacggc
     2461 atagacaccc agatcctcaa agcccagaaa cctgcggatc agcaccacgt caaagcggat
     2521 caggagagtg atgaagaggc tggaaagaaa cactctccag cccattgagt aggccctgag
     2581 gatatagccg ggacgaaagc ctttccagaa acgggagaac agctcactgt ggacgtaagc
     2641 ccccacggca caagccatag ccagattgta ggagatcagc acatacaaga gcctgtcgct
     2701 gttcctgaaa gccagatagc cgcccgccac catgatcagc atcacaaaac tgttgagggc
     2761 ctggtagtgg gagttggttt tcaccagatc ccgccccaga taatacatct gcatgtgcaa
     2821 ctgcagtttg gtgagtgaga tcgctcccgc caccaggact acgatgggag cagtgaagtc
     2881 aaactttaaa gcctggctga agaagggcat aaacgccagt cccacagcag ctatgagcgt
     2941 aaactcgatc agaaactgca gcagcgacca ggtgaacagc tgcaaccgct gctctgcctc
     3001 cttgcggatc aggtagggat aggtcttgtg gatgccgagg tccagcacca gccaggcaaa
     3061 gctactgatt gtgatcaaat agctgtactg gcccttgagt tccaccccca ggaagcgggt
     3121 ggaaatccat cccgtcagaa aagacagagc catcgtcagc accttatagc tggcgctgat
     3181 caggatgttt ttcttgaggt cgatgccggg cacggctgtt acagcatcac cttgaggata
     3241 tctctgagcc gcagtccttt gtcaccggtt tgatagcttt ccaacagggc tatcatgagg
     3301 cggggcgtgg ccagcccgga gccgttgagt gtgtgcacaa aggccggctt gccatctgga
     3361 ccgcgatagc ggatattggc ccggcgagcc tgaaaatctc tgaaagtact cacggacgag
     3421 acctccagat actttccggt cccgggtgcc cagacctcca gatcataagt tcgggctgag
     3481 gcgaagctga ggtctccggt gcagagttcc aacacccggt aatgcaggcc aaagctctgc
     3541 aggatatgct ccgcgtccac cagcatctct tccagggcgg catcagactt ttccggctcg
     3601 gtaaagcgca ccatttccac cttgttaaac tggtgcaggc gctgtaaacc tttggtatcc
     3661 tttccataag agccagcctc gcgacggaag caagccgtgt aagccacgtg cttgatcggc
     3721 aggctggaca gggggagcac ttcacccgcg tagagattgg tgactggcac ttccgcggtg
     3781 ggaatcagaa agagatcgtc ttcatccaca tgatacatat cgttttccag cttgggaagc
     3841 tggccggtgc cggtcatggt ctggcgcgtc accagcagag gcacgttcag ctcttcgtag
     3901 ccgtgatgat cgatgtgata gttgagcata aagttgatca gggcccgctc cagttgggca
     3961 cccttgcctt tgtagaccgg gaacccggaa ccgctgatct tggccccgcg ggccaggtcc
     4021 aaaaactgat tggcttccgc gatggcgaga tggtcggcgg gctcaaactc aaaggaaggc
     4081 ttttcacccc agcttttcac cacccggttg gcgctctcat ccctgcccac tgggacattt
     4141 tcagccggga tgttgggtac cgtcaacagc atgccttcca agcggttgct cacctccgtc
     4201 agctcctggt taagggcttt cacctcgtcc gccacccggg ccatttcttc cagtagctgg
     4261 ctggcatcag ctttggctcg tttgagagtg gcgatctcgg ttgagacctg attttgtctg
     4321 gctttgaggg tatcaaagtc gaactgcagc ttgcggcgtt tgtcatccca aaccaggatg
     4381 gcatcgatat ccgccttctc gtttttgttg gctaccgcgg ccttgaccag ttcggcgttg
     4441 gcgcggataa atctgatgtc taacatctat gctccgtaat aatgagttat tgagggttga
     4501 gtaggcaggc tggtttcccg cagattacgc agattttctc gcagatcacg ctgatattga
     4561 taagattgag agggttgagg attaacggct gtcgctttca cgccgctctc cttgccatct
     4621 gcgaaatccg cgtgcaaatc cgcgtaatct gtgggaacct gcaccggtcc tcgttcgcgt
     4681 ctctcattct tatgtattaa cacccgctct caaagcggta ttgatcatgg cctcaaaatc
     4741 cccgatcttg agagacgctc ctccgatcag gccaccgtca atgtccggtt gggcgatgag
     4801 ctcgctgatg ttttcaggtt tcatactgcc gccgtagagg atgtggatgg aatccgcgat
     4861 ctcagttcca aagcgatccc gcagccattc tctgataaaa gcgtgggctt cctgagcctg
     4921 gagggaggta gcagttttcc ccgttccgat tgcccagacg ggctcataag cgatcacgat
     4981 atctctgccg ctagtcaggg ttacttcctc aaacgcgccg ttgagctggg cccgcagcac
     5041 cccttcggtc tggtcggctt cgcgctccac aatggtctcg ccgatgcaga gcaggggcgt
     5101 gatccgcagt tcaaagagac gcagcagctt atcccggatc agacggtccg attcgtgatg
     5161 atactgacgc cgttccgagt ggccaactat gcagtgggca gtgcccagcg aggccagcat
     5221 cgtagcactg acttcgccag tgtaagctcc atcctgatga ggactcacat cctgagcgca
     5281 gacttgcaca aggctgtccg cgagcgcgct gagagcgatg ctcaaaaaag gatagggggg
     5341 tgccacaata acttgtaccc cggccaggaa ttggcccttg agcatgtggg ccagagacgt
     5401 gcagaattcc gccgtcgtaa aggcatcttt attcatcttc caattgccgg caactatgat
     5461 atctcgcatg cttactccat ggtaaaactt gattttgttc catcccatca ggaggggcgc
     5521 tttcgtcaag cactttcggt taaagtttta gagttttagg gttttagggt tgtgaaggtt
     5581 gagaggtaac agcgtgcgcc ggtccgggtt cccgcagatt nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn cgggaaacca gcctgccttc tcaaccataa atcctcaaaa
     5761 ccctacaacc ccctgccgct tttgtgcttg acatcatatc ctctccccaa aacggtgaag
     5821 tcatcgcatc tcaaccttgg gaggaaccga tgccgtctcg acctattagc ttgttgtttc
     5881 tggctgttgc cgtgtttttg atcattgcaa acagcctcag cgccacccag cccttttgga
     5941 gcatggagcc cagcaccaga gtgatcgacc gggccgattc actcaccggt ttcgacatca
     6001 ccaaatacac catcaacctc tatgtgaacg attccaccca ctacatagag ggcgtagtgg
     6061 atgccgaagt gactgccgaa gcgccgctta gctccattca ataccgtctg gaggggggca
     6121 gcctgagcgt gaccagcgtg ctggtggaca acgtctctgt tccctttacc caccaaaacg
     6181 gcatcatcaa tatcccggtc agcttcagca ccggccagag ttttatgacc agagtcagct
     6241 acaacggcat cccgggaaac agtcccgctc cttacaatat cggtttaaaa ttcacctcca
     6301 caggcatcta cactctttcc aatccggatg ccggacgctt ttactggccc agttacgacc
     6361 atccctggga taaagctttg gtggattgga acatccgcgt gaggtcagat tggctggcag
     6421 ccgccaatgg catcaggaca ggcatcaccg acaatggcga cggcacccgt acccatcatt
     6481 gggtttgttc ttttcccgta gcaacttacg tgatgggatt cgccgccgcg gcctacattg
     6541 aatccaacca gactgccggc gctttgccga tccaaaactt cgtgttgccc aatcaactca
     6601 acaacgctca ggttgatttt gccaatgtgc ctcagatgat cgcccatttt tccgagctct
     6661 acggtcccta tccttttgaa aaatatggcc atatggtggt gaacatgagc acttacgccg
     6721 ccatggaaca tcagaccatg accacctttg gagctcaata ccttaccggt aatcagaact
     6781 atgaatcgat tgtggcccat gagcttaccc accagtggta tggcaactac ctcaccccca
     6841 tcacgatgcg ggaggtgtgg ctgaaggaaa gctttgccac ctattcggaa ttcctctggg
     6901 cccacaaaaa gagcggctgg gattccgccc tgcaatatct gcgcaactct atccagcagt
     6961 attacataag ctgggaaaac tccaacggtc cccacactat cttcgatccc gtttacaatc
     7021 tcatgttcgc tccgcccacc tatgaaaaga gcgcttccgt gctccacatg ctgaggctca
     7081 aaatgggcaa cccggctttc ttcaacttca tccgcgctct cctgaccacc tatcccaacg
     7141 gcaatcttaa caccgcggag ttcgtagccc tggcccaaca acacagcgga caggacctca
     7201 cgcagttttt ccagcagtgg atctactctc ccggcatacc ggacggacgc ttttgtatct
     7261 ttcacaacgg caacggtatg gctaaagtct atgctcgttc ccaaagtccc accgccacgc
     7321 agttctggct ggatgtgcct ctcaaactta caggatcggc tgtggcggat tctgtggtga
     7381 tctcagcagg tcccacttgg acctcaaacg tgtttgccat tgggctggac gacgatcctc
     7441 aggacatcca aatcgatccc aaccattggg tgcttaccag atcttttgcc actatcgatg
     7501 ctcaactcac agcctgcctg gcatatagcg gagcagtgtc tgtgaattgg aactccctgg
     7561 gagtcgattt tccgctggcg ggataccatg ttttccgcaa aaccctgccc gacggccccc
     7621 tggttcaagt caccccaagt cctcagaccg gaatgagtta cacggatatc agcgtggtca
     7681 acggcagttc ttatgaatac tacgtctgcg cggttgatcc ggatggatat atctcgctgc
     7741 cctccaatcg gatgcaggcc agccccatca ctttcccctt tgatctggga tttctggtgg
     7801 tggacgaaac ccgagccgga aacggtaccg ccttttcacc cacggacgct caggtggacg
     7861 atttctatgc caccgcgctg caaggattta actatacaga gtgggacctg gatacccaag
     7921 ggaatccccc cctttccacc ctggcccgct atcccctcat attctggcac tcagacgact
     7981 tcagcgagat ccatctggca gagttgcaag accttgtcgg ttcctatgtg ctgagcggcg
     8041 gcaagctcat cctcagcggc tggaaacatc ccagcgtgtt caacgacgga tttttatccc
     8101 gttttctgcc tgggatcaca cccaacctgc aaaactcggc cgtctttgtc gccgcccatt
     8161 cagacacgta tcccagcctc tatcccgatc catccaaatt agccgcaccc tggaacggta
     8221 tgctgcccat gacctacacc tttagcggag ctgacgaggc cttgtacaca gcggaaatcg
     8281 tcgatgacgg tcccggagat ggagaagcgg ccgcgctcag aattcaggca gggggagaaa
     8341 tggtgctgct gggctttccg ctctatttta tgctcccgga tggggttagc gctttcctgc
     8401 aggacctcgt gccggaactc tatcccctgg tggaatccga tgatgaagtc aatgtgccgg
     8461 cgccacttag cctgagcctc tatcccaatc cctacagagg aaaaggcagc ctcagcctgg
     8521 gcctcaaagc tgcctccctg atcagcctcg acgtctataa cctgagaggc caaaaagtgc
     8581 tggccattca ggatttagtg ccaaaaaccc agggctcgga actgctctac gagataccgt
     8641 ccagctatat cgacaagctc tccagcggct gctatctgat cagagcaaag accagcaaag
     8701 gtatcctaac ccgcaggatt agcctcatca agtaagatgt tgcgcaacaa aggctcaggg
     8761 ctgcag
//
LOCUS       DAOSFN010000107         8757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6839_length_8757_cov_7.900368, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000107 DAOSFN010000000
VERSION     DAOSFN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6839_length_8757_cov_7.900368"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1730)
                     /locus_tag="PLO35_07275"
     CDS             complement(<1..1730)
                     /locus_tag="PLO35_07275"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOH79507.1"
                     /translation="MSLTLLSSCSLIRVKPQARDLQTRLRDFPSEDLPLQGKAELLWN
                     EYQVPFIKADHDQDAALLLGMVHAHLRLGQLYLFREVVNHRLASHLGPLATRVDHSLM
                     AMGVFDAVDSIATRLEPHDREWIQAFVDGLNLYQARMKTMPLELKALKIKPSPWSLSD
                     ILRMGRLISADVNWFNWLGALKLEEETSWKRYWNEIGSQPGGTLLSSETDPARKLFAD
                     LLSNLARTGSNALVIGPSRSKTGSALMASDPHLGMNLPNMWLLAGYQCPSYHVLGMMF
                     PGLPAVLVGRNPHISFSGTNMRSASSDLVALGPDEIARLRVVEKRLVVKGWPDKTVKL
                     RYSEWGPVVSDAPLFRDKRRVIVLRWVGHQASNEIGAALRMNQATNWEEFRASFADYA
                     VSGQNFLYADKANHIGLVPAVRIPVRSYERAPSIAISSGNRDQMWQAYLSPLDLPYLL
                     DPESAYIASANNQPLKASTPLGFAFSSDDRIRRMDEVIGATNKLGLKELMDLHRDTVI
                     PSARSAAGWFTSRLSGFVTSDKDDPATGVITALKGWDGDYRIDSKAPVIFELLLYYTA
                     KEYYTARYGK"
     gene            complement(1761..2306)
                     /locus_tag="PLO35_07280"
     CDS             complement(1761..2306)
                     /locus_tag="PLO35_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79508.1"
                     /translation="MKKYIASIILLAALGGLFAQTGLFDISYEDPWPHADSLLAKFGY
                     LAENIEGPMVKYYSKQDPMVEAVILFINPDNLKVAGWFVKHRNTLSSEMDQIVVDRVY
                     DMHGESTHYDRATEQLIWVLSPKRSIHLLYVSDGSLCVLYSDSDYEDLFIVDKTKLTE
                     DNPQPVEANPQVPKQPIPTPE"
     gene            complement(2233..2595)
                     /locus_tag="PLO35_07285"
     CDS             complement(2233..2595)
                     /locus_tag="PLO35_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79509.1"
                     /translation="MNHNTSHPFLLPGLRVYDVSRQVSELADHPPSLVAVEIPCALRC
                     FSPPAAPQLYGNYAAGMGLAASNNIKVIATQLFIDGKTDPEMMANLILQGGINEKIHC
                     LDHPAGRSGRALCPNWPV"
     gene            2755..3945
                     /locus_tag="PLO35_07290"
     CDS             2755..3945
                     /locus_tag="PLO35_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOH79510.1"
                     /translation="MIEVIDQKCIGCRACIKACAYDAIRVIDKLAVIDMDKCVLCGAC
                     VTACPVDAIVMRKKSSIQVDKDDYQGIWVFAEQREGILAPVTLELLGKGRELSDEMGT
                     ELSAVLLGSDIEALTSELIAFGADQVIVVDDPELRHFRDERYTKALSELAIKYKPAVI
                     LAGATVTGRSFIPRVAIELRTGLTADCTGLEYDAENANLLQTRPAFGGNIMATIVTAN
                     HRPQMATVRHKVMNPIARDESRSGIVIREQIVFDFDEEESVWLGFEKEKTTLVNITEA
                     NLIVSGGRGIKEAKNFALVEELAEALDGAVGASRAAVDAEWIAYAHQVGQTGKTVKPN
                     IYIAVGISGAIQHLAGMSSADYVIAINKDPDAPIFKAADLGIVGDLFEILPKLTKRVK
                     ELRS"
     gene            3984..5150
                     /locus_tag="PLO35_07295"
     CDS             3984..5150
                     /locus_tag="PLO35_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH79511.1"
                     /translation="MERNVALRLRASGSEHSEPLVVPGAIAYVPLINSHDHLVGNWVP
                     RAGDKRPYPNSHIWVEDMKESFSFHERNNFWANDGSFKLCEGGALHMALLGSYKNLFS
                     GCGTVQDHAPVQDSCYYNSFPVNVISAFRQCHSITLGNWWGGKTASEEMHLSHGKLPF
                     VIHLAEGVDDLTAREFSELEKQGLLKDNTLMIHGIALSEAELERIAAAGASVCWCPSS
                     NYYLIGETLKIDLALKHGVNVVIGTDSTMSGGVNLIAEFDVIHQKHPGLPLNLLYRMV
                     TENAVRALKLPARYGNLDPENCENLLLVDGVDQDPFENLLIMEAASIQLMVVDGIARF
                     GDLEWMERLKLEEDKYTTFRTGNKEKFVLGDPMELNDEVDAALGYHKDFPYLPF"
     assembly_gap    5213..5312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5355..6077)
                     /locus_tag="PLO35_07300"
     CDS             complement(5355..6077)
                     /locus_tag="PLO35_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOH79512.1"
                     /translation="MPRPHPLTQIFIALWFSGLAIVSVKPAMLAVLLLLCTALLLIGK
                     ILTPMTLGRSLLRVLPLIMMILLIQLLAVRKGTELCGLGSFVLYTQGLALGIALSLRI
                     TVILLAAKLLIRLTPMDFDAAFRTLRLPEEVGFMVSYAVHLLPGIATMLRDANLLIRK
                     RGIALRSLHVKQRIRLYGIIALRLVSELLQGSETRAIALELRGFRNGIKSSRLVTYRF
                     SWIDPLIAATLIVGAVLLLNIQ"
     gene            complement(6077..6766)
                     /locus_tag="PLO35_07305"
     CDS             complement(6077..6766)
                     /locus_tag="PLO35_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH79513.1"
                     /translation="MSSEPFLEARRLWFSYPERAALIKDLSLSLDRSRLTLLSGENGC
                     GKSTLLKLLAGDLKPGGGEILWQDQPLPNDKTMFFRQIWHMEQESARQRVGICPRHDL
                     EIWDMAFEQACRKGATNHKSDSISWSDAAARFNLEPAMLDLPYHQLSTGESRATVLMP
                     LLRTKSHYWLLDEPMSGLDGARATSFLQVCAEKLSEGVGMLIVTHTPEAYNDLPHIHL
                     ELRDGILWELE"
     gene            complement(6756..>7325)
                     /locus_tag="PLO35_07310"
     CDS             complement(6756..>7325)
                     /locus_tag="PLO35_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79514.1"
                     /translation="ELVLIKGRNGSGKSTLLNCVAGVIPEHIPALGKIMVQLDDIALH
                     ALPLRHKSPWLAYQMADPALQLLFPNIEKELSFALENIGMAASGMRLRIANALHSLDL
                     ENYCSPTLDPHLLSQGQKKLLMLAVCQAMETPVILLDEPSASLSARGRETLIKWVAGL
                     LQNGRMILAAEHHPCLDGLAAGTIDLNEL"
     assembly_gap    7326..7425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7426..8180)
                     /locus_tag="PLO35_07315"
     CDS             complement(<7426..8180)
                     /locus_tag="PLO35_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79515.1"
                     /translation="MKTLLAILSLMTFINLTALTVTDITGLSKEYDINALASLPRLEL
                     NASSEKEGKIKEHSFCGIRFDQWLYEQGFQSFGKIRFESGDRYRVSLTKAEMDSLECW
                     LVFASGGKEYEPGSSRLVFPALREMTWIKDVERVILEAQASVPIPVIYHRLDHMLQGM
                     QLIKDPAPFVNTEGWFLEFLLPKSELTLYLISADGLMAELEWPRHLEGAILEKYGDKI
                     NLKSPQIPGGMWIKDLVYIQYGDTVLIRDKALP"
     gene            complement(8177..8710)
                     /locus_tag="PLO35_07320"
     CDS             complement(8177..8710)
                     /locus_tag="PLO35_07320"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOH79516.1"
                     /translation="MFKRFSTRDLIVISTLAAIGIAIKPLVGPLFKMISTPLAIPGGS
                     LAGGFYMLWLSLAVFMVGKTGTGTLFGVVQALIVMVAGLQGNQGALSLISYPLPGIVA
                     DLLYLLFKDGDKIISHVLICAVANLAGALLVAVLLFHHPLALVFTIGGLALLSGAVGG
                     LVSKGLYAQLKKYGMIT"
BASE COUNT         2021 a         2274 c         2336 g         1926 t
ORIGIN      
        1 tttttgccgt atctggctgt gtagtattct tttgccgtgt aatagaggag gagttcaaag
       61 attaccgggg cctttgagtc aatccggtaa tcaccgtccc agcctttgag agcggtaata
      121 acacccgtgg ccggatcatc cttatccgat gttacaaaac cacttagcct ggaggtaaac
      181 cacccagcgg ctgaacgcgc ggagggaatc accgtatccc gatgtaaatc cataagttct
      241 ttcaggccaa gcttgtttgt agccccgatc acctcatcca tgcgccggat cctgtcatcg
      301 ctggaaaagg caaaacccag cggtgtcgat gctttgaggg gctggttgtt ggcgctggcg
      361 atgtaagcgc tttccggatc aagcagataa ggcaggtcca agggagatag ataagcctgc
      421 cacatctggt cacgattgcc ggaactgatt gctatggaag gggctcgctc ataactccgc
      481 accggaattc tgacagccgg caccagaccg atgtgattgg ccttatcggc atagaggaag
      541 ttctgtccgg agacggcata atccgcaaaa ctggcccgga attcttccca gttggtggcc
      601 tggttcatcc tgagggcggc tccgatctca ttggaggcct gatgccccac ccagcggagc
      661 acgatcactc tgcgtttatc tctaaacagc ggcgcgtctg aaactaccgg accccactcg
      721 ctgtatctca gcttgacggt tttatccggc cagcctttga cgacgaggcg tttctccacc
      781 actctcagcc gggcgatttc atcaggaccg agcgcgacca gatcagaact ggcggagcgc
      841 atattggtgc cactgaatga gatatgcggg ttgcggccaa ccagcacggc tggcaacccc
      901 ggaaacatca tccccagcac atgataggaa gggcattgat agccagccag caaccacata
      961 ttaggcaaat tcatacccag atgaggatct gaggccatca gggccgaacc ggtctttgac
     1021 ctggaggggc ctatcaccag agcgttgctg ccagtcctgg ccaggttgct gagcaggtcc
     1081 gcgaagagct tgcgggctgg atcagtttct gagctcagca gagttccacc tggttggctg
     1141 ccgatctcgt tccagtatcg tttccagctt gtttcctctt ccagtttgag ggctcccagc
     1201 cagttgaacc agttcacgtc agcgctgatc agacgcccca tcctcaggat gtcggaaagg
     1261 ctccagggac tgggcttgat cttgagggct ttcagttcca atggcatggt tttcatccgc
     1321 gcctgataga ggttcagccc atccacaaag gcctgaatcc actctctatc gtgtggctcc
     1381 agtcgagtgg cgatgctgtc cacggcatca aagacaccca tcgccataag gctgtgatcc
     1441 acgcgggtgg ccaagggtcc cagatgcgag gccaaacggt ggttgacgac ttctctaaag
     1501 aggtagagct gccccaatct caagtgggcg tggaccatcc ccaaaagcag cgcggcgtcc
     1561 tgatcgtggt ccgccttgat aaacgggacc tgatattcgt tccagagcag ttccgctttt
     1621 ccctgcaagg ggagatcttc cgaagggaag tcacggagcc gggtttgcag gtccctggcc
     1681 tggggtttta ccctgatcag ggaacaggaa ctcaagagag taagactcaa tacaacaatc
     1741 agtaaagccc gcaaggctgt ttactctgga gtggggatag gctgtttggg gacttgggga
     1801 tttgcttcca ctggttgggg gttatcttcc gtgagctttg ttttatccac gataaacaag
     1861 tcttcatagt ccgaatccga atagagcacg cagaggctgc cgtccgacac atagagcaga
     1921 tggatactgc gtttgggact caatacccag atcagctgtt cggtggcccg gtcatagtga
     1981 gtggattcgc cgtgcatgtc atatacccgg tccaccacga tctggtccat ttcgctgctg
     2041 agggtgttgc ggtgtttgac gaaccagccc gccactttga ggttgtcagg attgatgaag
     2101 aggatcaccg cttccaccat ggggtcctgc ttggagtagt atttcaccat cggtccctcg
     2161 atgttctcgg ccagataacc gaatttggcc agcagcgaat cggcatgggg ccagggatct
     2221 tcataggaaa tgtcaaacag gccagtttgg gcaaagagcc ctcccagagc ggccagcagg
     2281 atgatcgagg caatgtattt tttcatttat tccaccttgt aagattaagt tggccatcat
     2341 ttccgggtcc gtttttccgt caatgaaaag ctgtgtggca atcactttga tattattact
     2401 ggctgccagc cccattcctg cagcatagtt gccgtagagc tgtggtgctg ccggcggcga
     2461 aaaacaccgg agagcgcacg gtatctccac ggcaactaat gagggaggat gatctgccag
     2521 ctcggaaacc tgccgggata catcataaac cctgaggcca ggcagcagga aaggatggct
     2581 ggtattgtga ttcattcgga gcatatatcc tttgccggta ggatgatagc atgctgccac
     2641 cgacctcgaa acgggggcta accagaatac atcatcgtcg cgcgggccaa gttcgcgatt
     2701 tccgcttgac aagaatttat ctcttaaaag gcttacgcgc aggagttttt gtatatgatc
     2761 gaagtaatag accagaagtg tataggctgc cgagcctgta tcaaagcctg tgcctatgac
     2821 gctatcagag tgatagataa gcttgccgta atcgatatgg acaaatgtgt cctgtgtggg
     2881 gcctgcgtta cggcatgtcc tgtggatgcc atcgtgatgc gcaaaaagag ctccattcag
     2941 gtggacaaag atgactatca gggcatttgg gtgttcgcgg aacagcggga aggcatcctg
     3001 gcccccgtga ctcttgagct tctgggcaag ggccgcgagc tgtctgatga aatgggcacg
     3061 gagttgagtg ccgttctgct gggcagcgat atcgaagccc tcacttcgga actgatcgct
     3121 tttggagcgg accaggtgat cgtggtcgat gaccctgagc tcagacactt tagagacgaa
     3181 cgttacacca aagctctcag cgagcttgcg ataaaatata aacccgctgt catcctggca
     3241 ggggccaccg tgactggtcg ctccttcatc cccagggtgg cgattgagct cagaactggc
     3301 ctcacggctg actgcactgg cctggaatac gatgctgaaa atgccaacct gctgcagacc
     3361 cgtcccgctt ttggcggcaa catcatggct acgatcgtga cggccaatca caggcctcag
     3421 atggcaacgg tccgtcacaa agtgatgaat cccatcgcca gagacgaatc ccgcagcgga
     3481 atcgtgatcc gggagcagat cgtgtttgat tttgacgagg aagagagcgt ttggctgggt
     3541 tttgaaaaag agaaaaccac tctggtcaac attaccgagg ccaatctgat cgtttcgggc
     3601 ggccggggaa taaaagaagc caaaaacttt gccttggtag aagagctggc ggaagccctc
     3661 gatggcgctg tgggagcttc ccgagcggct gtggacgcgg aatggatagc gtacgcccat
     3721 caggtggggc agaccggaaa gactgtgaag ccaaatattt acatcgcggt ggggatttca
     3781 ggggccattc aacacctggc cggaatgtcc tcagcggatt atgtgatcgc tatcaacaaa
     3841 gatccagacg cgccgatctt taaagcggcc gatctgggca ttgttggtga cctctttgag
     3901 atactgccaa aacttacaaa aagggtgaaa gaactgagat catgatcatc acgccttcgg
     3961 tcagtgtcat cagtcctgat cgagtggaac gaaatgtggc tttgcgtctg agggccagtg
     4021 ggtctgagca ctccgaaccg ctcgtagtgc caggggccat cgcttatgtg ccgctgatca
     4081 attcccacga tcatctggtg ggcaattggg taccccgggc aggagacaaa cggccttatc
     4141 ccaattctca tatctgggtg gaagatatga aagaatcttt cagcttccac gaacgcaaca
     4201 acttctgggc caacgacgga tccttcaaac tctgtgaggg cggggcactc cacatggctt
     4261 tgctgggcag ctacaaaaat ctcttttccg gctgcggaac cgttcaggac cacgctccgg
     4321 tgcaggattc ctgctattac aacagcttcc ccgtcaatgt gatcagcgcg tttcgccaat
     4381 gccactccat tacgctgggc aactggtggg gcggaaagac agcctcggaa gagatgcacc
     4441 tttcacacgg taagctgccc ttcgtgatcc atctggccga gggagtggac gatctcacag
     4501 cgcgcgaatt cagcgagctc gaaaagcaag gtcttctcaa agataacaca ctgatgatcc
     4561 acgggatagc cctgtctgaa gccgagctgg aaaggatcgc cgcggctgga gcttccgttt
     4621 gctggtgtcc cagcagcaat tattacctga tcggcgagac tctcaagatc gatctggccc
     4681 tcaaacatgg cgtcaatgtg gtgataggca ccgattccac catgagcgga ggcgtcaatc
     4741 tgatcgcgga atttgacgtg atccatcaaa aacatccggg gttgccgctc aatctgcttt
     4801 accggatggt gaccgaaaac gcggtcaggg ccctcaaact gccagccaga tatgggaatc
     4861 tcgatcccga gaattgtgaa aacctgttgc tagtggacgg agtggaccag gatccctttg
     4921 aaaacctgtt gatcatggaa gccgcttcga ttcaactgat ggttgtggac ggcatcgccc
     4981 gcttcggaga tctggaatgg atggaacgcc ttaaactgga agaagataaa tataccacct
     5041 tcagaaccgg caacaaagag aaattcgtgc tgggcgaccc catggaactc aacgatgagg
     5101 tggacgcggc gctgggctat cataaagact ttccatatct gccgttctaa ttgagaattg
     5161 agaattgaga attgagaatt gtttctccct ggcgcagcca tctcacctat gannnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaggacgc gctgcaggtc aggctgcagt
     5341 tacaactgca taagctactg gatattaagt aacaagacag ctccgacgat cagggtcgcg
     5401 gcaatcagcg gatcgatcca gctaaatctg taggtaacca ggcgactgct cttgatgccg
     5461 tttcggaagc ctctcagttc caacgcgata gcgcgagttt cactcccttg caggagctcg
     5521 ctcaccaatc tcagcgcgat gatcccatag agcctgatcc tctgcttcac gtgcaatgaa
     5581 cgcaacgcta tgccccgttt gcggataagc agattcgcgt ctctgagcat agtggcgatc
     5641 cctggcagta aatgaacagc gtaagatacc atgaatccca cctcttcggg aagtctgagc
     5701 gtcctgaaag cggcgtcgaa atccatggga gtgagccgta ttaaaagctt ggcggccaac
     5761 aggatgactg tgatccgcaa gctcaacgcg atacccaggg ccagaccttg agtataaagg
     5821 acaaagctac ccagtccgca aagctcagtt ccctttctga ctgccaaaag ctggatcaat
     5881 aggatcatca taatcagagg aaggacgcgc aacaaagatc tgcccaatgt catgggagtc
     5941 aatatcttgc ctatcagaag cagggccgtg cagaggagca acagcactgc cagcatggcc
     6001 ggcttgacgg atactatggc caacccgctg aaccacagcg cgataaatat ctgggtcagg
     6061 ggatgcggtc ggggcattat tccagctccc acaggatgcc gtcccggagc tcaagatgta
     6121 tgtggggcaa gtcgttataa gcttccggag tatgggtcac aatcagcatt cccactccct
     6181 cactcagttt ttcggcacag acttgcagga agctggttgc cctggcgcca tcaagcccgg
     6241 acataggctc atccaagagc caataatggc ttttggtccg gaggaggggc atcaatacgg
     6301 tggccctgct ttcacccgtg gagagctgat gatagggtaa atccagcatc gctggttcca
     6361 gattgaaacg tgcggccgca tcagaccagg aaatcgaatc agacttgtga ttggtcgcgc
     6421 ccttcctgca ggcctgctcg aaggccatgt cccaaatctc cagatcgtgg cggggacaaa
     6481 taccgacccg ctgtctggca ctctcctgtt ccatgtgcca tatttgcctg aaaaacatag
     6541 tcttatcatt tggcaacggc tggtcttgcc acaaaatctc tcccccgcca ggtttcaggt
     6601 ctccagccag cagcttgagc aaagtgcttt tgccacagcc attttcgcca ctcaggaggg
     6661 tgagccttga tctgtccaga ctcaggctca ggtctttgat cagcgcagct ctttccggat
     6721 aggaaaacca gagccgacgg gcttccagaa aaggctcaga gctcatttag gtctatggtc
     6781 ccggcggcca ggccatcaag acaagggtgg tgctccgcgg ccagaatcat cctgccgttt
     6841 tggagcagcc ccgccaccca tttgatcagg gtttcacgtc cccgcgcgga aaggctggca
     6901 gatggttcat ccagcaagat cacaggcgtt tccattgcct ggcagacggc cagcatcagg
     6961 agctttttct ggccctgaga tagcagatgg ggatccagcg tgggggaaca gtaattctcc
     7021 aaatccagac tatgcagggc attagcgatt ctcaaccgca ttccggaagc tgccatcccg
     7081 atgttttcca gggcgaagct gagttctttt tcgatgtttg ggaagagaag ctgcagagcg
     7141 ggatcggcca tctgatatgc cagccagggg gatttgtgac gtaaaggtaa ggcgtgcaga
     7201 gcgatatcat caagctgaac catgatcttt cccagggctg gaatgtgctc tgggatcacc
     7261 cccgccacgc agttcaacag cgtgctttta ccgctgccgt tcctgccttt tatcaggacc
     7321 agctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntgtgg caacgctttg
     7441 tctctgatca gaacggtgtc tccatattgg atatagacca ggtctttaat ccacattccg
     7501 ccagggattt gagggctttt gaggttgatc ttgtcgccgt acttttccag gatcgcccct
     7561 tccagatgac gcggccattc cagttccgcc atcaggccgt ccgcgctgat cagataaagt
     7621 gtgagctcgc ttttgggaag caggaactcc aaaaaccagc cttctgtgtt gacaaagggg
     7681 gcggggtctt tgatcaactg catcccttgc agcatatggt ccaatctgtg ataaatcact
     7741 ggaataggaa cagatgcctg agcttccagg atgacgcgtt ccacgtcttt gatccaggtc
     7801 atttctctca gggccggaaa caccagtcgg gaactgcccg gctcgtattc cttgcctcct
     7861 gaagcgaaga ccagccagca ttccaggctg tccatctcgg ccttggtgag gctgacgcga
     7921 tagcggtctc cggattcgaa gcggatcttg ccaaagctct gaaaaccctg ctcatagagc
     7981 cactggtcaa agcggatgcc gcaaaagctg tgttccttga tcttgccctc tttttcgctg
     8041 gaggcattca actccaacct tggcagactg gccagagcat tgatgtcata ttccttgctc
     8101 aggccggtga tatcagtcac tgtgagggcg gtcagattga taaaagtcat cagtgacagg
     8161 atcgcaagca gtgttttcat gttatcattc cgtatttctt taattgcgca tagagccctt
     8221 tgctgaccag gcctcctacc gctccggaaa gcaaagccag gccgccaatg gtgaacacaa
     8281 gagccagagg atgatggaag agcagaacgg ccaccagcag cgctccggcc agattggcga
     8341 ccgcgcagat caggacgtgg ctgatgatct tatctccatc tttgaaaagc agatacaaca
     8401 ggtcggccac gatcccgggc agaggatacg aaatcaggct cagtgctccc tgattgccct
     8461 gcaggcctgc caccatgacg atgagggcct gcaccactcc aaaaagggta ccggttcccg
     8521 ttttgcccac cataaacaca gccagactca gccacaacat gtaaaagcct ccggccaggg
     8581 aacctcccgg aatcgccaaa ggggtggaga tcatcttgaa aagcgggccc acgagcggtt
     8641 tgatggctat gccaatggcc gccaaagtgg agatcacgat cagatctctg gtgctgaaac
     8701 gcttgaacat ctttcctcat tacttgattt tgccgatcta atatgtttcc cgcagat
//
LOCUS       DAOSFN010000108         8664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6939_length_8664_cov_10.767337, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000108 DAOSFN010000000
VERSION     DAOSFN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6939_length_8664_cov_10.767337"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="PLO35_07325"
     CDS             complement(<1..1628)
                     /locus_tag="PLO35_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH79517.1"
                     /translation="MITYNECLKKLVELGKESGYITFKQINDILPSSPFFLDKVDDII
                     FDLQQDGIEIIDETEKRLTKGGAALRKPAAPKKFKTKRYYDDPVRMYLREMGRVPLLD
                     REGEVRVAKKIETYQKMINQNVFKSGSTLREMYNFLHRYRERRVRLDQIFKMDIGAWI
                     DKNQDARIIDVFKDILKANEGTLDKIGIILDNCDFDDTGTANRQEEIDALRDNIIDTF
                     STLNYNDKLIKRMVYRVRSLVERIEESYNSINELTRIKRYTIDEICTFGRRAKKSDED
                     FDEVRSETSMDPNIFVETLRKIKNARRKIRRVELETKMTGSELVFLLREIDRAERNKE
                     KAQNEMIEANVRLVISIAKRYNNRGLEFLDLIQEGNTGLMRAVEKYDYRKGYKFSTYA
                     TWWIRQAITRAIADQARTIRIPVHMIESINKINRTSRRLMQKLGREPYPEEISEVLDM
                     PVDKIKNILSISKEPVSLDKPIGHDSEDSILGDFIEDRTIISPERIAERSLLKKQVDE
                     VLKTLTTREERVIRLRFGIDDGYHRTLEEVGNIF"
     gene            complement(1658..3391)
                     /gene="dnaG"
                     /locus_tag="PLO35_07330"
     CDS             complement(1658..3391)
                     /gene="dnaG"
                     /locus_tag="PLO35_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOH79518.1"
                     /translation="MENHLIDQIRSANDIVEVIQAYLPLKHVGSNWRGICPFHQDTTP
                     SLYVSQPKQIFKCFACGKAGNVLTFVQDYEKLSFIEAVKKLAARVGIPIPEYERTTKV
                     DTKRDQLLLIYKTARDFFAENLFRHGAQVLKYLENRQISAETAKNLELGYALNGDKAL
                     LNHLMKEGHSVSLLKESGLFGNYSGNLVDMFRDRLMFPIHNNTGDVIAFGGRVLDANA
                     PGGKYVNSPGTELYTKGKELYGLFKTKYEISKADSVLVCEGYFDFLRLYGSGFVNSVA
                     SLGTALTEDQIYLLNRYTKKIYMLYDGDTAGIRNAVRAGSICLGKGLEPYVVELPPKE
                     DPDSFLLQHGSDALKEKIATAKSLIGFVAYHPGIAGGPSERIDLLLDPIRVNPDAVRR
                     ELLVKEVSEAFGISAAALNSRLRKVSRAPVTPEPQSPAPQHTGLEERPEERFLLILSL
                     QDRDNYKELASQVSEDYFSNRLYRDIFRWLMDHITPEAVEDPSSILNNIDNAQIRDAL
                     AELLFEDLHDLRFGDTLNQVRIRKLQRDLEEIDRKILAEPQNVELFKQKQELSRAYRR
                     MTSKVVHKIRF"
     gene            complement(3391..5742)
                     /locus_tag="PLO35_07335"
     CDS             complement(3391..5742)
                     /locus_tag="PLO35_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOH79519.1"
                     /translation="MRQSYQDLEYAVLTSQIASRCHSSLGAALAAELKPLDDLSAIKA
                     ELVLNSQAQEALKRGIEPQLADLSDPAPLYEDGAQLVFGYEEFFAFYQNAVISCLTSA
                     YRDAADDLSNLISLIKRVHPLPQVRDRFLAIFDAEGEVKDSASSELSRIRKRANSLRG
                     SIMKTMNGLLADQGMQAHLQDKFFTQREDRYVLPVRESSVNRVPGIVQSHSGSRSTIF
                     IEPQSVVPLNNELQLIKQDEKKEIYRIFSEFSQDIRDLKDVFVANTKALARLDLLFAW
                     ARLGNQIKAELPSLSPDPVLELSGARHPLLSLKLGHHQVIPFDLTIGDEKRVLVLSGP
                     NTGGKTVLMKAVGLITLMTLTGLPTPLSPQSRIGMFDKVFADIGDDQSIESALSTFSS
                     HVAKIRAMLEGATPRSLVLIDEIGAATDPQQGSGLAQAMLERFVELGCRCVVTTHYTA
                     LKIFAESEAAALNASMQFDLAGMTPTYRFAPGFPGDSFAIEVAASLGLEPSLIERAKS
                     LSGSQNREFTELLRKMQEEKKALARESYEYRLKNRNLEARLGELDTRSASLDEELKAR
                     KQKFLKELQMELISRQKLYQKELSELKDLDKAQRKSLSEKKLKETESELGSLSNQIME
                     AAKAGRKAAFDPKPGDRVWLANFESDAIILERRDGQVLVDMNGISFKTPLDTVFEGSQ
                     PLPDQELIGSASVRGKASAPARVSTELKLLGCTFDEAQPLIDEFIDTAIVAGLHRLRI
                     VHGKGTGVLRSKTRDYLRRKKQVISIDTPPPSEGGSGVTVVSI"
     gene            complement(5757..6302)
                     /locus_tag="PLO35_07340"
     CDS             complement(5757..6302)
                     /locus_tag="PLO35_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOH79520.1"
                     /translation="MGAIDWAILALLAVCVFLGWRKGLAAALINLAGVIAGFFLVGHF
                     YPLVARSLRIKYELSTGLSTLLAVLLITILLAVVVRLVIYALNRVLKALKLSSLNRFA
                     GMLLGFANGLLILICVTLVLDYFPKVSTPLKDSSQHRVYAGVNLLKDELIGSLKLKQH
                     LEYLELKAKALKLPDLPGDKE"
     gene            complement(6420..6626)
                     /gene="rpsU"
                     /locus_tag="PLO35_07345"
     CDS             complement(6420..6626)
                     /gene="rpsU"
                     /locus_tag="PLO35_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOH79521.1"
                     /translation="MPTVVAKENESFDFLLKRFKKKCEKAAILSEIKKNQAYEKPSVK
                     KKREANAARRKMLKVQRRMQRYMR"
     assembly_gap    6720..6819
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7126..8247
                     /locus_tag="PLO35_07350"
     CDS             7126..8247
                     /locus_tag="PLO35_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79522.1"
                     /translation="MAVTVKPVSSKAEMKTFIYLPRKIHVNHANWVPPLWSDDARVLN
                     PKRNPAHEYCDSIYALAWKNGEAVGRIAGIVNHRYNEHAKFQNARFGFLESPDDPEVT
                     KALLDFVENWARQQGMNKIVGPMGFTEEDPEAFIIDGFDQIPNLATYQNLPYLLHHLD
                     ACGYAKEIDYMVYKLDIAGAMTDLYRRIYERVSRNKEFTLIEFKNKAHLKRYMHRIFD
                     LMNASFLHLYGYSPLSPSEVESLAKRYMPLLDPRFIKAVVNPKDEVIGFIVAIPNLAE
                     GIRKAKGNILPFGIFHIMAASKRSTKLDTYLGAIAESYRGKGVDALMGYSLLRSARNA
                     GYKYLDSHHEMETNTQMRSEMERNGGVIYKKYRIFQKEL"
BASE COUNT         1801 a         2246 c         2467 g         2050 t
ORIGIN      
        1 tggaagatgt tgcccacttc ttcgagggtg cggtgatagc cgtcgtcgat gccgaagcgg
       61 aggcggatca cgcgttcttc acgggttgtg agggttttga ggacttcatc cacctgtttt
      121 ttgaggaggc tgcgttcggc gatgcgttcc gggctgatga tggtgcggtc ttcgataaaa
      181 tcgcccagga tgctgtcttc gctgtcgtgg ccgatgggtt tgtcgaggct gacgggctct
      241 ttggagatgg agaggatgtt tttaatctta tccaccggca tgtccagcac ctcggagatc
      301 tcttccggat agggctcgcg gcccagcttt tgcatgaggc gacggctggt gcggttgatc
      361 ttgttgatgg attcgatcat gtggacgggg atgcggatgg tgcgggcctg gtctgcaatg
      421 gcgcgggtga tggcctgacg gatccaccag gtggcgtagg tgctgaattt atagcctttg
      481 cgatagtcgt atttttccac ggcgcgcatg aggccggtgt tgccttcctg gatgagatcg
      541 aggaattcca ggccgcggtt gttgtagcgc ttggcgatag agatcacgag gcggacgttg
      601 gcctcgatca tctcgttttg ggccttttcc ttgttgcgct cggcgcggtc gatctcgcgc
      661 aggaggaaga cgagctcgga gccggtcatc ttggtctcaa gttctacgcg gcggatcttg
      721 cggcgggcgt ttttgatctt gcggagggtc tccacaaaga tgttggggtc catgctggtc
      781 tcggagcgca cttcgtcgaa gtcttcatcg cttttcttgg cgcggcggcc aaaggtgcag
      841 atctcgtcga tggtatagcg tttgatgcgg gtgagctcgt tgatggagtt atagctctcc
      901 tcgatgcgtt ccaccaggct gcggacgcga tagaccatgc gcttgatgag cttgtcattg
      961 tagttgaggg tggagaaggt gtctataatg ttgtcacgca gggcatcgat ttcttcctga
     1021 cggttggcgg tgccggtgtc gtcaaaatcg caattgtcca ggatgatgcc gatcttgtcc
     1081 agggtgcctt cgttggcctt gaggatatct ttgaagacgt cgatgatgcg ggcgtcctgg
     1141 tttttatcga tccaggcgcc gatgtccatc ttgaagatct ggtccaggcg cactctgcgt
     1201 tcacggtagc gatgcaggaa gttatacatc tcgcggaggg tgctgccgga tttgaagacg
     1261 ttttggttga tcatcttctg ataggtctcg atctttttgg ccacgcgcac ttcgccttcg
     1321 cggtccagga gcgggacccg gcccatttcc ctcagataca tcctgacggg atcgtcataa
     1381 tagcgcttgg tcttaaactt tttgggcgcg gcgggtttgc gcagagcggc gccgcctttg
     1441 gtgaggcgtt tttccgtctc atcgatgatc tcgatgccat cctgctgaag atcgaagatg
     1501 atgtcatcca ccttgtccag aaagaaggga ctgctgggga ggatgtcgtt gatctgtttg
     1561 aaggtgatat agccggattc tttgcccagc tccaccagct ttttcaaaca ctcgttatag
     1621 gtaatcatca atgctccttg atatgaaggc caggccctca aaaacggatt ttgtggacga
     1681 ccttgctggt catccgtcga taggcgcggc tgagctcctg tttttgtttg aaaagctcca
     1741 cgttttgcgg ttccgccagg atcttacgat caatctcctc caggtctcgc tggagcttgc
     1801 gaattctcac ctgattcaag gtatccccga accgcaggtc gtgcaggtct tcaaacagta
     1861 gttctgccaa agcgtccctt atctgtgcgt tatctatatt attcaaaata gaggaaggat
     1921 cctccaccgc ttccggagtg atatggtcca tcagccagcg gaagatatcc cggtatagcc
     1981 tattactaaa ataatcctca ctcacttgtg aagcaagctc cttgtaatta tcacggtcct
     2041 gaagagatag gataagcaaa aaacgttcct caggccgctc ttccaatccg gtatgctgag
     2101 gagccgggct ctgcggctct ggagtgacgg gcgctctgga gaccttgcgc agacggctgt
     2161 tcagcgccgc ggcggagatc ccaaaggcct cggagacttc cttgacgagg agctcgcgcc
     2221 tcacggcatc gggattgacg cggatggggt ccaggagcag gtcgatgcgt tcactgggtc
     2281 ctccagcaat gccgggatga taggccacga agccgatcag gcttttggct gtggcgattt
     2341 tctctttgag agcatcgctg ccgtgctgga gcaggaagct atcgggatct tccttgggag
     2401 gcagttccac cacgtagggt tccagacctt tgcccagaca gatggagccg gctctcacgg
     2461 cgttgcggat gcctgccgtg tctccgtcgt agagcatata gatctttttg gtatagcggt
     2521 ttagcagata gatctggtct tcggtgaggg cggtgcccag agaggccacg ctgttcacaa
     2581 agccgctgcc gtagagcctc agaaagtcga aatagccttc gcagaccaat acgctgtccg
     2641 ccttggatat ctcatatttg gtcttgaaga ggccatagag ttccttgccc ttggtgtaga
     2701 gctcggtgcc gggagagttg acgtattttc cgcccggagc gttggcgtcc aggaccctgc
     2761 cgccaaaagc gatcacgtct ccggtgttgt tgtggatggg aaacatgagg cggtcgcgaa
     2821 acatatccac gaggttgccg ctatagttgc caaagaggcc gctttccttg agcagcgaga
     2881 cggagtgccc ctccttcatg aggtggttga gcagggcttt atcgccgttg agggcatagc
     2941 ccagctccag gtttttggct gtctcggcgc tgatctggcg gttttccaga tacttgagca
     3001 cctgagcgcc gtggcggaag agattttcgg caaagaaatc gcgggccgtc ttgtagatca
     3061 gcaggagttg gtcgcgcttg gtgtccacct tggtggtgcg ttcatattcc gggatgggga
     3121 tgcccacccg ggccgccagt ttcttcaccg cctcgataaa gctaagcttt tcataatcct
     3181 gcacaaaggt gagcacgttg ccagccttgc cgcaggcaaa acacttgaag atctgcttgg
     3241 gctgggaaac gtagagagag ggggtggtgt cctgatggaa gggacagatg cctcgccagt
     3301 tgctgcccac gtgtttgagg ggcagatagg cctggatgac ctccacgatg tcgttggcgg
     3361 acctgatctg gtcgattagg tggttttcca tcagatactt accacggtca ccccgcttcc
     3421 accttcggaa ggaggagggg tatcgatgct gatcacctgt ttcttgcggc gcaggtaatc
     3481 gcgcgttttg cttctgagga cgccggtgcc cttgccgtgg acaatgcgga gccgatgcag
     3541 gcctgccacg atggcggtgt cgataaattc gtcgatcagc ggctgagctt cgtcgaaggt
     3601 gcagcccagc aacttgagtt ccgtggatac tctggcggga gcgctggcct tgcctctcac
     3661 ggaagcggag ccgataagtt cctggtcggg aaggggttgg gagccttcaa agacggtgtc
     3721 cagtggggtc ttaaaactga tcccgttcat atccaccagc acctgtccgt cccggcgttc
     3781 cagaataatg gcgtcggatt caaagttggc cagccagacc cggtctcccg gtttgggatc
     3841 aaaagccgct ttgcgtccgg ctttggctgc ttccatgatc tggttgctga ggcttccgag
     3901 ctcggattcg gtttccttga gtttcttctc ggagagggat ttacgctggg ccttgtccag
     3961 gtccttgagc tcgctgagct ctttttgata gagcttctgg cgggagatga gctccatctg
     4021 cagttccttg agaaacttct gtttgcgggc cttgagctcc tcgtccagac tggcggaacg
     4081 ggtgtccagt tcacccagcc gggcttccag attgcggttt ttgagccggt attcatagct
     4141 ttcgcgggcc agggctttct tttcttcctg catcttgcgc aaaagctcgg tgaattcgcg
     4201 gttctgggat ccgctcaagc ttttggcccg ctcaatgagg gaaggctcca atcccagaga
     4261 agccgccacc tcgatggcaa agctgtcgcc cggaaagcct ggagcaaaac ggtaggtggg
     4321 ggtcatgccg gccagatcga actgcataga agcgttcagg gcagccgctt cgctttcggc
     4381 aaagattttg agggcggtgt agtgggtggt gaccacacag cggcagccca gctccacaaa
     4441 gcgttccagc atcgcctggg ccagacctga accctgctgg ggatcagtgg ccgctccgat
     4501 ctcgtcgatc aagaccagac tgcgtggcgt ggcaccctcc agcatggccc tgatcttggc
     4561 cacgtgagac gaaaaggtgg aaagagcgct ctcgatggac tggtcgtccc cgatatcggc
     4621 aaagaccttg tcaaacatac cgatccggct ctgagggctg aggggagtgg gcaaaccggt
     4681 gagcgtcatc agcgtgatca gccccaccgc tttcatcagt acggttttgc caccggtgtt
     4741 gggaccactg agcaccagca cgcgtttttc atccccgatt gtgaggtcga aagggatcac
     4801 ctgatggtgc ccgagcttta ggctgagaag gggatgacgt gctccgctga gctccagaac
     4861 gggatccggg ctcagggacg gcagttccgc tttgatctga ttgcccaggc gggcccaggc
     4921 aaacagcagg tccagcctgg ccagggcttt ggtgttggcc acaaagacgt ctttgaggtc
     4981 ccggatgtcc tggctgaact cactgaaaat gcggtagatc tctttctttt cgtcctgctt
     5041 gatgagctgt agttcgttgt tgagcggcac cacgctctgg ggctcgataa agatggtgga
     5101 gcggctgccg gagtggcttt gcacgatgcc gggcacccgg tttacggagc tttcccggac
     5161 gggcagcaca tagcggtctt cccgctgagt aaagaattta tcctgcagat gcgcttgcat
     5221 gccctggtcc gccaggagcc cgttcatggt cttcataatg ctgccgcgca gggagttggc
     5281 acgcttgcgg atgcgggaca gttcgctcga agcgctgtct ttgacctcac cttcggcatc
     5341 aaagatggcc agaaagcggt cccgtacctg cggcaatgga tggacgcgtt tgatgaggct
     5401 aataaggttg gacagatcat ccgcggcgtc tctgtaagcg gatgtcaggc aggagataac
     5461 ggcattctga tagaaggcaa agaattcctc atagccaaag acgagctgag cgccgtcctc
     5521 atacagagga gcgggatcgc tgaggtcagc cagttggggc tcgattccac gcttcagggc
     5581 ctcctgggcc tgggaattga ggaccagttc cgctttgatg gcgctaaggt cgtcaagggg
     5641 cttgagttcc gccgccaggg cggcacccag gctgctgtga cagcggctgg cgatctggga
     5701 tgtgaggacc gcgtattcca ggtcctgata gctttgacgc ataggctaag cgcgctttat
     5761 tccttatctc caggcaaatc cgggagcttc agtgctttgg ccttgagctc cagatattcg
     5821 agatgctgct tcagtttcag gctgccgatc agttcatcct tgaggaggtt gacgccagcg
     5881 taaacacggt gctgggagct gtccttgagc ggggtggaga ctttgggaaa gtagtccaga
     5941 accagagtaa cgcagatcag gattagaaga ccgttggcaa agcccagcag cattccggca
     6001 aagcggttca gagagctgag cttgagggct ttaagcaccc ggttgagggc atagatcacc
     6061 agccgcacca caaccgccag caagatcgtg atcaacagca cggccagtaa ggtggacaga
     6121 cctgtggaaa gctcgtattt gatcctcagg ctgcgcgcca cgaggggata aaaatgaccc
     6181 accaaaaaga atccggcgat cacacccgcc agattgatca gggctgctgc caggcccttg
     6241 cgccagccca aaaacacgca tactgccagc aatgccaaaa ttgcccaatc tatcgctccc
     6301 atctgtcaca ctcccgtcaa tgcgctatgg ttatggatta ttggctaaaa acggcataca
     6361 gggatgggct ccctgtatgc tttcaactga taaacgattt aaggtatgag cgcccggcct
     6421 tacctcatgt agcgttgcat tctgcgctgc actttcagca tcttgcggcg cgcggcgtta
     6481 gcttcgcgtt ttttcttcac gctgggcttc tcgtaagctt ggttcttctt tatttctgaa
     6541 agaatagcgg ctttctcgca tttctttttg aagcgtttca gcagaaaatc aaaggactcg
     6601 ttttctttag ctacgactgt cggcaaacaa atcaccacct ttttttacag atttgagagt
     6661 gccacttttt tggaggacgc aatggtgtca agcacaaagt gttagaggag tgagagctgn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tctgcttttc tctgtgttta
     6841 ataacgttac gcgttacagg cacgcgccga ataggaattt gacgctacag gtccagtcta
     6901 acgagggcgt ctaaggcttc ccaagccagc tccccgtaat cctcaattct caattctcaa
     6961 ttctcaatta accctccctc cgtatctctc tccgtttcct ttgtagctct gtagtgaaaa
     7021 aagaaagctc tgcttctccg tgataaatct aaaccacttg acgtaattcc cccctcccct
     7081 tgagtggtat ttttcctgtc agaatatttg gataaggatg ctatcatggc tgttaccgtc
     7141 aagcctgttt cctcaaaagc tgagatgaag accttcatct acctgccccg caagattcac
     7201 gtcaaccacg ccaactgggt gccacctctc tggtcggacg acgcccgggt gctcaatccc
     7261 aaacgcaatc ccgcccacga atattgcgac agcatttacg ccctggcctg gaaaaatggc
     7321 gaagccgtgg gcagaatagc cggaatcgtc aatcatcgct acaacgagca tgccaagttc
     7381 caaaacgccc gtttcggctt tctggagtca ccggacgatc ctgaagtcac caaagcgctg
     7441 ctcgactttg tggaaaactg ggcccggcaa cagggcatga acaagatcgt ggggccgatg
     7501 ggcttcaccg aagaggatcc cgaagccttt atcatcgacg gttttgacca gatccccaat
     7561 ctggccacct accagaacct gccctatctg ctgcatcacc tcgacgcctg cggatatgcc
     7621 aaagagatag actatatggt ctataaactc gatatcgccg gggctatgac agacctctac
     7681 cgcaggatct atgagcgggt gagccgcaac aaggaattca cgctcatcga gtttaaaaac
     7741 aaagcccacc tcaaacgcta tatgcaccgc atcttcgacc tcatgaacgc cagtttcctg
     7801 cacctctacg gctacagtcc tctctcccca tccgaggtgg aatcgctggc caagcgctat
     7861 atgccgctgc tggacccacg cttcatcaaa gcggtggtca accccaaaga cgaggtgatc
     7921 ggcttcatcg tggccattcc caacctcgcg gaaggcatcc gcaaagccaa gggtaacatc
     7981 ttgccgtttg gcatcttcca catcatggcc gcctccaaac gcagcaccaa actggacacc
     8041 tacctgggtg ccatcgcgga aagctatcgc ggtaaaggag tggacgcgct gatgggctac
     8101 agcctcttgc gcagcgccag aaacgcaggc tacaagtacc tggacagcca tcacgagatg
     8161 gaaaccaaca cccagatgcg cagcgaaatg gaacgcaacg gcggagttat ctacaagaaa
     8221 taccgcatct tccaaaaaga gctgtaaggc tagcctggag agtgcactac agagatacgg
     8281 aggcatcttt tttactacag agctacaaag gaaacggagg gagatacaga gaaagggggc
     8341 ttgattgaca attgagaatt gacaattgag ggaggcactt ggcttgtgga gcagcaaacg
     8401 tccccgtttg cggtggcctg aggagacgct cggaggcata ttcagctcgg aataaatgat
     8461 acataacagc atgaattaca agcagtttga ctgctgccac gattctccca gacaatcagg
     8521 atgatgtgtc accctgatat acaaagacaa ttgagcatat ctgtatcact gtctttagtg
     8581 cttgtgcgat actgccgcaa caacgacagg cctaaccccg ttggagttca agcggccacg
     8641 ataaagctat gcatgacaac gagt
//
LOCUS       DAOSFN010000109         8633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6961_length_8633_cov_9.720797, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000109 DAOSFN010000000
VERSION     DAOSFN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6961_length_8633_cov_9.720797"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      229..351
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            384..1193
                     /locus_tag="PLO35_07355"
     CDS             384..1193
                     /locus_tag="PLO35_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79523.1"
                     /translation="MREKSFLLVLMAVLLLLSACSEKDKPTDTTIYGYKLDQFVSADS
                     VTAYVDASAELPNNFRHLFNYEIVGEDGFSPRASSNAGYDLSFGQFSEGYLVPTDDGK
                     TWFPTLDLPGAFKVRDTAFFRLYRKVDVDDGLRGSKSIELRGLNVYNMPNWDSEGEDA
                     IKLADLLQGIAAYDSVAFVAVDDYTKVYTPELVNDGYYFMRSEVTSFPSFNAQLPGAM
                     KKFKKLARIVVYGASGAQNHDFPLANNTLADMVFQVPADLSGYERVIMESN"
     gene            1201..3102
                     /locus_tag="PLO35_07360"
     CDS             1201..3102
                     /locus_tag="PLO35_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH79524.1"
                     /translation="MLKRLSVVTIALVAMGGMQAVGTPAVQDSLPPRSYLLPTVRVVA
                     DSPLEALASVKRVEPSKREAAITLYEGLQKLPGISNTVGTKDESNLRLRGFRKNELKL
                     MVNGRPLSAGYFGNVDVGNFSLDGIAELQIISGPAAAMYGGNNLGGVVNLIRRDPSDD
                     EWGSLSLTARRNNTNRLSLSSAHRFNNWSYRLAASREHSDGMALSQSFEPTGFENGGI
                     RDNQFKTLWNLDADVYMHLSEWQQLGISGGWGWIPKKRLPSSVFEASYRQYLDWRRQN
                     LTLMWEGVMGQNLSGAAMLYYDGGGDRYQEFNDSGFTNLGMDSVMRHHSVGLAPRFKL
                     DLHPGSDLQFGLNSQLSYNERQDNGFYTEWSDHTMGQHNAWLQWQFQPSRLSINAGLG
                     LSAWHHDEREGLKAFLDPSLGLSWKHDEDSSSRISIGINSSYPTMRQLFSSQKGNPLL
                     DAQRAFKTELAHSASLMIGSVRSLGEINLYYNSARDLIDLQGERYQNIYRVDSWGLES
                     SLLLVPVPDWEFSLAYAYLEYSASSDYRLTESPRNSLVAELSLPLVYGIRLKLNSGWN
                     DIRHSQDASSDYHVLPAYWKHDLSLGRVLNKTTVSLGLENLLDANYFTEYGYPAPGLD
                     FFVKLSQAF"
     gene            3314..4561
                     /locus_tag="PLO35_07365"
     CDS             3314..4561
                     /locus_tag="PLO35_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOH79525.1"
                     /translation="MNNIQAKLERLMALGNMMTELKDTDVLMENILTEARKLCACDAG
                     SIYIREGNELSFQFAQNDTQQRDLPVGKKLIYSTYRMPINEKSIAGYAASHNAVLNIP
                     DVYQIGDDVPYSFNSSYDLKTGYRTGSILSVPLRTSSAKIVGVLQLINAMDPSGQVVP
                     FDPEDLPIIKHFANMAAVALERAEMTRAIILRMIGMAELRDPTETGAHVNRVGAYSAE
                     LYEAWALKHQVAPDEIQRKKDLLRIAAMLHDVGKVGIPDAILKKPGGLTDEEFDVIKT
                     HPVIGARLFIDPNSELDTISRDIALCHHERWDGSERGYPGFVDIDSGAPLPEYLQPNG
                     KPQKRAAEEIPLWARIVSIADVYDALSCHRSYKEPWTQDQVFDELQKSSGSQFDPELV
                     EIFVARREEMQAIRARYPDPHSE"
     gene            4658..5284
                     /locus_tag="PLO35_07370"
     CDS             4658..5284
                     /locus_tag="PLO35_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79526.1"
                     /translation="MKPVLLPLLLVIIFMAGGCKDGASIRVVNRSDHNLYVQVENGSE
                     VVIPGQGEHNWDIDTDTESFLSGHVEKDVIVRIVGETYHIFDEWEEVYTDSAQMVLRA
                     GETRNVYINPNRASIKIVNQSSQAMNGAVLYRHNFVNASTVAVLGELQPGELIFKRVD
                     YATPTTQFYYYAIVQMADGTVYEFGGPDSVLDKDQQLLITLEDPEPQG"
     gene            5341..8346
                     /locus_tag="PLO35_07375"
     CDS             5341..8346
                     /locus_tag="PLO35_07375"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH79527.1"
                     /translation="MKGKRLYLALILLVVAVFACALTPEQASRPLPQDPEVLSGRLSN
                     GMSYYILNNKKPENRAELRLYVNAGSVNEDDDQRGLAHFTEHMAFNGTKSFGRTEVVD
                     YLTSIGMGFMNGLNAGTSYEFTYYMLKVPTDNAEQLRTGIQILSEMAHAVTYAPEELE
                     RERGIILEEWRLGQGPQQRVSDATMKVLFQGSKYAERAPIGTEEVLRNFKRETIQRFY
                     GDWYHPANQSVVIAGDFDPDAMLALLKEFFAPIPAKENPRVLEKIVIPENVEPQVTVV
                     TDPEFPYNLAQIIWKRENRPSITMGDYYDQLKQDLYCDMLSSRLEELSKKPNPSYSMA
                     FAMSQNILKGLGGAAAIAIAPQGKSEEAFQVLLTEAQRVERFGYLPSEFDRAKLRLTR
                     RVEQAVAQKATRQSGDVAEGFINTLYYGGAYISPEQEESMVKSLLAELSLEELNVLGP
                     TIFTDKNMFISITGPESAKASLPTQERLLAIVGEAKTQELTAYTEEVIDKPLIPEKLK
                     PGTIKKQLTNRKAGINIWTLSNGVKVYFKKTDFKQDEVLLGAWSPGGFSYSDATSLSS
                     ANLLSMYVSESGFGEFDATALQKLTAGKVANANLSLSLNSEGFNASCSPQDLELMFQM
                     VHQFGTNPRFDEDDFTSFLQRMKGVLENSLLDPQSYFFDLLESAPYNDNPYTRSLHPD
                     DLDKADLETVQRIFKDRFADFSDFSFVIVGNYDEAKIKDLVRTYLGSLPASQREESYK
                     DVGIRPIQGISENRFQRGLSDRCFATLITTSPYKGDVATRSKLHALMYLYNEKLRENV
                     RENLSGVYVVQAWPAQERYPSPHLVTNAFLACDPLRVDELMNAIVLTADSLRQGLFED
                     RYVEAAKITLIKTYEDRIKTNDYWLMGIINGIAEDRPIDAFLSFPRYYQEINRQTVID
                     AANMFLPYDRNLHKLVMMPDDIGIPVEVIGGASPLEQAIEVIEPELPLEVETEVEASA
                     MEPPVEEPALIEIEVTPGEVEPEANDTSN"
BASE COUNT         2123 a         2440 c         2257 g         1813 t
ORIGIN      
        1 aaaatcctgc cctcttccct attcctccct gcatcctatt acggactcat tacggactta
       61 gtccgtaatg agtccgtaat gataccgtaa tgccaataag caggaaagca cagacagtta
      121 gataatagta gcgtaaatac atgtaatgca aggtattaaa ttatctcaga caattatccg
      181 ggtcgaacac tctcaagaac atccttgaca aaggcagcgg cctgattatt tcagcactca
      241 cgccgagtgt actcccctaa accacattgt cggcaggggg aatcaccgtt tagggtatgg
      301 ctggaaacgg ccctgccttc cgatttgaaa aggcgggatg ggaaaactat aaccacctgc
      361 ctccacgcga catggaggta aaaatgcgcg aaaagagctt tctgctcgtt ctaatggcgg
      421 tcctgctgct gctgagtgct tgcagcgaaa aggacaagcc caccgacacc accatctatg
      481 gctacaagct ggaccagttt gtcagcgctg acagcgtgac cgcctatgtg gatgcctcag
      541 cggaacttcc caacaatttc cggcacctct tcaattacga gatcgtgggt gaggatggtt
      601 tcagtccccg cgccagttcc aacgccggtt atgatctgtc ttttgggcag ttcagcgaag
      661 gttatctggt gcctaccgat gacggcaaaa cctggtttcc cactctggac ctgccgggag
      721 ctttcaaggt acgcgacaca gcctttttcc gtctgtatcg caaagtcgat gtggatgacg
      781 gtctccgcgg ctccaagtct atcgagctga gaggcttgaa tgtttacaac atgcccaact
      841 gggatagcga gggtgaggac gccatcaagc ttgcggacct cttacaaggt atagctgctt
      901 atgactcagt ggcgtttgtg gctgtggatg actatacaaa agtttacact cccgagctgg
      961 tcaatgacgg ctattacttt atgagatcgg aagttaccag cttcccgagc ttcaatgccc
     1021 agcttccggg agccatgaag aaattcaaaa agctggcccg gatagtggtt tacggcgcgt
     1081 ccggagctca gaatcatgac ttcccgctgg ccaacaatac cctggcggac atggtcttcc
     1141 aggttcccgc cgatctgagc ggttatgaac gcgttataat ggaaagcaac tgatctgatc
     1201 gtgctcaagc gtctgtcagt agtaacgata gcccttgtgg ctatgggtgg aatgcaggct
     1261 gtcgggaccc cagcggttca ggacagcctg cctccccgca gctatctgct tcccaccgtg
     1321 agagttgtgg cggattcacc cttggaggcc ctcgcgtctg tcaagcgggt cgagccctcg
     1381 aaaagagaag ccgccatcac gctctacgaa ggcctgcaaa agctgccagg catcagcaac
     1441 accgtgggaa ccaaggatga gagcaacctg cgcctcagag gcttccgcaa aaacgagctc
     1501 aagctgatgg tgaacggccg gccgctcagc gccggatatt ttggcaacgt ggatgtgggc
     1561 aatttcagtc tggacgggat cgcggagttg cagatcatca gtggtccggc cgctgccatg
     1621 tatggaggca acaacctggg tggagtggtc aatctgatca ggcgggatcc ttcagatgat
     1681 gaatggggtt ccctgagcct gacggccagg cgcaacaaca ccaaccgcct cagcctcagt
     1741 tccgctcacc gcttcaacaa ctggagctac cgtctggccg catccagaga gcactcagac
     1801 ggcatggccc tctcccaaag ctttgagccc accggctttg agaatggcgg catccgtgac
     1861 aaccaattca agaccctctg gaacctggat gccgatgtgt atatgcatct gtctgaatgg
     1921 caacagcttg gcatcagtgg aggctggggc tggatcccca aaaaacggct gccctcctcc
     1981 gtcttcgagg cttcctatcg ccaatatctg gattggcggc gccagaacct caccctgatg
     2041 tgggaaggcg taatgggaca gaatcttagc ggtgcggcaa tgctatatta cgatggaggt
     2101 ggggaccgct atcaggagtt caacgattcc ggcttcacaa acctgggcat ggacagcgtg
     2161 atgaggcatc acagcgttgg gttggccccc cgcttcaagc tggacctcca tcccggctcc
     2221 gatctccagt ttggcctgaa ctcccagctc agctataatg agcgccagga caacgggttt
     2281 tacacagagt ggagcgatca taccatgggc cagcataacg cctggctgca gtggcagttc
     2341 cagccctccc gtctgagcat aaatgccggc ctgggcctca gcgcctggca ccatgacgag
     2401 cgggaaggtc tgaaggcttt tctggacccc agtctgggcc tttcctggaa acacgatgaa
     2461 gacagcagca gcagaatctc gatcggcatc aatagctcct atcccacgat gcgacagctc
     2521 ttttcgagcc agaaaggtaa tcctctcctg gacgcgcaac gggcttttaa aaccgaactg
     2581 gcccactcgg cttctctcat gatcgggagc gtgagatcac tgggagagat caacttatac
     2641 tacaattccg cccgcgacct catcgacctt cagggcgagc gctatcaaaa catctaccgg
     2701 gtggattcct ggggcctgga aagcagcctg ctgctggtgc cagttccaga ttgggagttc
     2761 agtctcgcct acgcctatct cgagtatagc gccagtagcg attaccggct caccgaaagc
     2821 cccagaaaca gcctggtggc ggagctcagt ctgccgctgg tttatggaat ccgactgaag
     2881 ctcaattccg gctggaacga catccgccac agccaggacg cctcttcaga ctatcatgtg
     2941 ctgcccgcct actggaaaca cgatctcagc ctaggccgcg ttttgaacaa gacgactgtc
     3001 agcctgggcc tggaaaacct gctggatgcc aattacttta ccgagtatgg ctatcccgct
     3061 ccgggactgg atttctttgt caagctgagc caggcgttct agggaattga caattgagaa
     3121 ttgaaaattg agaattattc cacccatggg gccgaaaatg agagtcaatt ctcaattgtc
     3181 aattctcaat tctcaatttc tccgttcctc tgttttcaaa aaaaagaaac aaaccctctt
     3241 tttctcttgc cagaaacctg cccccgaaaa tagtgtaagc tgcatgcatt aatacagaaa
     3301 aagtgggagt cgcatgaaca atatacaagc caaactggag cgtctgatgg ccctgggcaa
     3361 catgatgacg gagctcaagg ataccgatgt tttgatggaa aacatcctca ccgaggcacg
     3421 caagctctgc gcttgcgacg ctggcagcat ttacatccgg gagggcaacg agttgagctt
     3481 tcaattcgcc caaaacgata cgcagcagcg tgatctgcct gtgggcaaga agctgatcta
     3541 cagcacatac cgcatgccca tcaacgagaa atccatcgca ggctacgcgg ccagccacaa
     3601 tgccgtcctc aacatcccgg acgtctatca gatcggcgat gacgtgccat acagcttcaa
     3661 ctccagctac gacctgaaga ctggttaccg caccggcagc atcctcagcg ttcctttgcg
     3721 cacctccagt gctaaaatcg tgggggtatt gcagttaatc aacgccatgg acccttccgg
     3781 ccaggtagtc cccttcgatc ctgaagacct gcccatcatc aaacactttg ccaatatggc
     3841 ggccgtggca ttggaaagag cggaaatgac ccgcgccatc atcctcagaa tgatcgggat
     3901 ggcggagctg cgggacccca ccgagacggg agctcacgta aaccgcgtag gcgcttattc
     3961 cgcggagctt tatgaggcct gggccctcaa gcaccaggtg gccccggatg agattcagcg
     4021 caaaaaagac ctgctgcgca tcgcagccat gcttcacgac gtgggcaaag tagggattcc
     4081 ggacgccatt ctcaaaaaac cgggtggcct cacggacgag gaatttgatg tgatcaagac
     4141 ccatcccgtg atcggagccc gtctctttat cgatcccaat tccgagctgg acaccatctc
     4201 ccgcgatatc gcgctctgcc atcacgaacg ctgggacggc tcggagcgtg gatatccagg
     4261 ctttgtggat atcgacagtg gcgctcccct gccagaatat ctgcagccga acggcaagcc
     4321 tcaaaagcgc gccgcggaag agattcccct ctgggcccgc atcgtttcta tcgcggacgt
     4381 ctatgacgcg ctgagctgcc accgctccta caaagagccc tggactcagg accaggtgtt
     4441 tgatgagctg caaaaatcct ccggcagcca gttcgatccc gagctggtgg agatctttgt
     4501 agccaggcgg gaagaaatgc aggccatccg ggccaggtat ccagacccgc atagcgaatg
     4561 agaggccgcg cttggtgagg accttgttag gccgaaaagt caaaaaacca cttgacatca
     4621 ttgggtctct tacatttctt agccaaacga ggtatatatg aaaccagtgc ttctaccatt
     4681 attgctcgta atcatattta tggcaggcgg atgcaaagat ggtgccagta tcagagtggt
     4741 caaccgcagt gaccacaatc tctatgtgca ggtggaaaac ggctctgaag tagtcattcc
     4801 gggccagggg gagcacaact gggatatcga taccgacacg gaaagcttcc tgagcggtca
     4861 tgtggaaaag gacgtgatag tccgcatcgt gggcgagacc taccacatct tcgacgagtg
     4921 ggaggaggtc tataccgatt ccgctcagat ggtgctgcgg gctggcgaga cgcgcaacgt
     4981 ctatataaat cccaaccggg ccagcatcaa gatcgtcaat cagagctccc aagctatgaa
     5041 cggagcggtg ctctaccgtc acaactttgt aaatgccagc actgtagccg ttttgggaga
     5101 gctgcagcca ggtgagctga tcttcaaacg ggtggattat gccaccccca ccacgcagtt
     5161 ttattattac gcgattgtgc agatggccga tgggacggtt tatgagtttg gcggtccgga
     5221 tagcgtcctg gacaaagacc agcagctgtt gatcacgctg gaagatcccg aacctcaagg
     5281 ctagcatatc ctgggactaa gtacccatat tatagcaaaa tacatcatga aggagtcaca
     5341 atgaaaggga aaaggcttta tttagctttg atcctgctgg tggtggccgt atttgcctgc
     5401 gcgctcaccc cggaacaggc ctctcggcct cttcctcagg acccggaagt cctgagcgga
     5461 agactatcca acggcatgag ttattacatc ctcaacaaca agaagccgga aaaccgcgcc
     5521 gagctgaggc tctatgtgaa tgccggttcc gtaaatgagg atgacgacca gcgcgggctg
     5581 gcccacttta cggaacacat ggccttcaat ggcaccaaga gctttggccg cacggaggtg
     5641 gtggattatc tcacctccat cggcatgggc tttatgaatg gcctcaacgc cggcaccagt
     5701 tacgaattta cctactacat gctcaaagtc ccgaccgaca atgccgagca gcttcgcacc
     5761 ggcatccaga tcctttccga gatggcccac gccgtcacct acgctccgga agagctggag
     5821 cgcgagcgcg gcatcatcct ggaagagtgg cgtttaggcc aaggacccca acagcgcgtt
     5881 tcggacgcca ccatgaaggt gctcttccag ggctccaaat atgccgaaag agctcctatc
     5941 ggcacagaag aagtattgcg caacttcaag cgcgagacca tccagcgttt ctacggcgac
     6001 tggtatcatc ccgccaatca gagtgtggtg atcgcgggag attttgatcc cgacgcgatg
     6061 cttgccctgc taaaggaatt cttcgctccc attcccgcca aagagaatcc cagagtgctg
     6121 gaaaagatcg tgatccccga gaacgtcgaa cctcaggtca ccgtggtcac cgatccggaa
     6181 ttcccctaca accttgccca gatcatctgg aaacgcgaga accggcccag catcaccatg
     6241 ggtgattatt acgaccagct caagcaggac ctctattgcg acatgctcag cagccgtctg
     6301 gaagagctca gcaagaagcc caatccttcc tactcgatgg ctttcgctat gagccagaac
     6361 atattaaaag gcctgggagg agctgccgcc atcgccatcg ccccccaggg taaaagtgag
     6421 gaagctttcc aggtgctgct cacggaagcc caacgggtgg aacgctttgg ttacctgccc
     6481 agcgagtttg accgcgccaa gctgagactg acccgcagag tggaacaggc tgtggcccaa
     6541 aaagctaccc gccagtctgg tgacgtggcg gaaggcttta tcaataccct gtattatggt
     6601 ggggcctata tcagccccga gcaagaagaa agcatggtca aaagcctgct cgcggagctc
     6661 agcctggaag agctgaacgt cctgggaccc acgatcttta ccgataaaaa catgtttatc
     6721 tccatcacgg gaccggaatc agccaaagcc agcctgccca cccaggaaag gctcctcgcc
     6781 atcgtcggtg aagccaagac ccaggaattg accgcctaca cggaagaggt gatcgataag
     6841 cccctcatcc cggaaaagct taaacccggc accatcaaaa aacagctcac caaccgcaaa
     6901 gccggcatca acatctggac cctctccaac ggcgtgaaag tctatttcaa aaagaccgat
     6961 ttcaagcagg acgaagtgct cttgggagcc tggagccctg gtggtttcag ctattcggac
     7021 gccaccagcc tgtcctccgc caatctgctc agcatgtatg tgagtgaatc cggatttggt
     7081 gagttcgatg ctaccgctct gcaaaaactc acggccggaa aggtggccaa cgcgaatctc
     7141 agtctgagcc tcaacagtga aggcttcaac gcgtcctgct ctcctcagga cctggaactg
     7201 atgttccaga tggtgcatca gtttggcacc aatccccgct ttgacgaaga cgacttcacg
     7261 tctttcctgc aaaggatgaa aggagtgttg gaaaactcgc tgctggaccc ccagagctat
     7321 ttctttgacc tcctggaatc cgctccctat aatgataatc cctacacccg cagtctgcat
     7381 ccggatgacc tcgacaaagc cgatctggag acggtccaga ggatattcaa agaccgcttt
     7441 gccgatttct cggatttcag ctttgtgatc gtgggcaact acgacgaagc caaaatcaaa
     7501 gacctggtgc gcacttatct gggcagcctg cccgcctccc aacgcgagga atcttataaa
     7561 gacgtgggca tcaggcccat tcaaggcatc agcgaaaacc gcttccagcg tgggctcagc
     7621 gatcgctgct ttgccactct gatcaccacc agtccctaca agggtgacgt cgccacccgc
     7681 agcaagctcc atgccctcat gtatttatac aacgagaagc tgcgtgaaaa cgtccgcgaa
     7741 aacctcagcg gcgtgtatgt ggtccaggcc tggccagccc aggaacgcta tccttcccct
     7801 catctggtca caaacgcttt cctggcctgc gatcccttgc gcgtggacga gctgatgaac
     7861 gccatcgttc tgacggctga cagcctgcgt cagggcctct ttgaagatcg ctatgtggaa
     7921 gccgccaaga ttactctgat caaaacatac gaagacagaa tcaaaaccaa cgactactgg
     7981 ctgatgggca tcataaatgg gatagctgaa gaccgcccca tcgacgcttt cctcagcttc
     8041 cccagatact atcaggaaat caaccgtcag accgtcatcg acgcggccaa tatgttcctg
     8101 ccctatgacc gcaacctcca caaactggtg atgatgccgg atgatatagg tatcccggtg
     8161 gaagtaatcg gaggggcttc accgctggag caggccatcg aagtgattga gccagaatta
     8221 cccttggaag tagaaacgga agtagaagca agcgccatgg agccacctgt cgaagagccc
     8281 gctctgattg agatcgaagt gactcccggc gaagtggaac cagaagccaa cgataccagt
     8341 aactaataat cataccttca tttatggccg gcaggatgag ctcttgccgg ctttttttct
     8401 gctttctcgg actgccaaat agagcagata aactggcttc atagcctggg cgcaaaggcc
     8461 aacctggagt tcaaagcccc tggtcagatt atacgtttat actaagcctg acggggcttt
     8521 ggacttcaag cagcacatcg gatagtatag tgcgcgagtg tgctggttga agtccaagcg
     8581 gcccgcaaac tcgctgtagt gcatggcttt atcgtggccg cttgaactcc aac
//
LOCUS       DAOSFN010000110         8627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6966_length_8627_cov_9.941437, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000110 DAOSFN010000000
VERSION     DAOSFN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6966_length_8627_cov_9.941437"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="PLO35_07380"
     CDS             complement(<1..1425)
                     /locus_tag="PLO35_07380"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH79528.1"
                     /translation="MITEEQAGVFVSENPEASLQDLLLKATELQQRDPEEALILISEI
                     AEQAKLHKLPSYLAAAQKVKGNVHFLRGATSEAKECYLAALAQARKVENPKLKGDINY
                     NLGRVFTREKDYPTALKYLHKSLTYRGRLDGRTDEISSLSHIGQVFWELQDFQQAVDW
                     YQRAARTCDSTVSDRLTSSIYNNLGNAYIKTNEISLAMEAYIRSLNLKEAWGNAADLA
                     TAKLNLGNLFYTSQDYPTALGYYQDAQRLYQEAGDLAREAIVLSNLGATFSELKDLDQ
                     AELCHLRALEYFRQYGMRENQAKSLNNIGATYHHKAQYRKAVDIYRESLEIKSDFYNP
                     ESLAVTYNNLSECHYNLEDYVSALENTQTSMSYANQIKSKALLLKNYLMLGKIYAALE
                     DYQNAYEALNVHRLLDLEVYAEGRESLLADKMVQYDTAGKSRQIDVLNSDRQALMELV
                     EKQVKDKIRYLKLYRAKLDEVKRRK"
     gene            1740..3266
                     /locus_tag="PLO35_07385"
     CDS             1740..3266
                     /locus_tag="PLO35_07385"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOH79529.1"
                     /translation="MKATHIEVGNLSDLGVNPAREKNEDYFGRFDGEFGTLLIVCDGM
                     GGHIGGEIASRLAVEAIQNYIAKYHIPTEENMIIAQSIEHAQQKIMEAVNDDPSLAGM
                     GSTLVMLLIREHQFWYAHLGDSRLYLRRDDAITRLTRDHSEVQNLVDLGVIKPEDART
                     HPRRSVLSKALGHDTGDPEISGPHMLYQDDVFLLCTDGLTEYLEDHELLDYMTESPQV
                     AAHNLVEEAKRRGGMDNITVQVIRVLHGTHKGRTEAPAEVSNRKPVNYKKYLVPALAV
                     ILGLYILAMIPRTCRKVFDRKPMTHEDSLRVAEKARIKQEKAEQKRLAKEAKQARKQD
                     KDAPAAQPSAQVDPALDSALAARLSAKPASADYQKFLDELRAANPSAKLPAKISFISD
                     QTDGRSVLVTPGQTIYFAWNYLDKGNKLNMEQLQYLITIAAAMGDASIAKSAPANAEG
                     YFSGSPSVIDEATLANARKLWVAAKPAGDYDFTRIGRSVNKNARKSGYTLIYTKPAAQ
                     "
     gene            3320..4234
                     /locus_tag="PLO35_07390"
     CDS             3320..4234
                     /locus_tag="PLO35_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79530.1"
                     /translation="MRKALMGILVLAIALPCLASVEVKSVQSRDAGKEANNVNIGLSG
                     DAAYQVQSSHDGKVHKIIVRDAQNAVARPDYQRLSPVIDRIRTYREGSNTVVEINTMQ
                     PSRISHSQSSDRISLRLDQGQEIPPEPEDSTPLAPQTPDLSVDRDPELPTKAAARVNP
                     TKLPAPTQASTPEPEKPIFPATIDLDSAPPPAEEREESAQAQPAEHPIKEFLRIHWSW
                     MLLLATGLAMLIYAVRACLRAKATHRPTNLEPEETPALIMDSATRQRMVMRLAEQGWT
                     ASQVAAELKLPVREVERIISKSKQRPEL"
     gene            4240..5859
                     /locus_tag="PLO35_07395"
     CDS             4240..5859
                     /locus_tag="PLO35_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79531.1"
                     /translation="MPRPRTCLILLGLLVLSCGLDASGTFSFARVSVPSMFDPQVSSQ
                     FDDNYLRYPYFLYYDAPLKAPQGVSHPFSSFKRLGDSIVDAFNPANRRYALLQYAYRD
                     SGMVFAPPELALNGVPQSKDAPRVGSMNFRSELNFVAGYDQRWEDDTRYGFIWKGIAL
                     KANINKHIAAKAIWTNGAFTGDRDAAEALSPLVDGYITHTAEQTRLDNLTARLSYYHP
                     NLGVELGRKSHNIGNNLSGSIVLSDQVNDYGYLKAEAMFGKLRVSLLHASLNADSARA
                     EAKLADLDLPDKYLALHEIAWYSDLHRFYAGEAVVYGNRAPDINYLLPHTFWRVTEHN
                     QWDRDNVLIYAGFSKSSYLSHSYWFTGLFDELSYGKLFSNWWGNKWAVQSGAEIYFPA
                     LELISLPPKLAVEVVAVRPWTYTHYQNHTMYSHDKKPLGYPKGSNLVSGTAELDYPLS
                     MNFHLVGQASFTRQGSEGNDWRLNYSDYFPPALIDTARTPWFAGQRTDTLELGSLLKI
                     YLAHHNLYIGHRAKKTDKWEHSVFAGWLFLY"
     gene            5929..6333
                     /locus_tag="PLO35_07400"
     CDS             5929..6333
                     /locus_tag="PLO35_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79532.1"
                     /translation="MPIVDRLPFPRKYKLHQGVVLLVIVAALFGLWLRSQSRKALGER
                     VIIHSIGIERYDTQFVEINFEIENKGKRAEDVMLLARIYDPAGDEIASKLFQIALKPS
                     WKGPRSHILDKMTRPLKLGEKPYRATMELRTR"
     gene            complement(6743..7870)
                     /locus_tag="PLO35_07405"
     CDS             complement(6743..7870)
                     /locus_tag="PLO35_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79533.1"
                     /translation="MHIYSGMRPGSSSSYHYFYAHAQDNPLVVNSIRRVYSYTCDGET
                     TTTDTTFVFGGSFEQHDGYFICRGPGGESSGTVPPYRSSRITNDGYYLQDDYSNDIAN
                     TDHVMRIWYQYNDDMKLTQAIYKRNLSSWSRLDCEVDSLGRRLQEVIYSSADSLIWVP
                     SSRVCYSYTGEPSGLEADFEKYMSYPPLRNAISGTTKPLYINNAWKIGTISSSYWSNN
                     EWEGDYPIPYVLHEMETGYYLYLGASYWAWDWHGLPTNLCSPNGYSDVPGYVIKYSHT
                     SQVEVDDELLLPVVRLAMWPNPSRGYSQVRLDLAVQTRVSVKTYNIKGQMLKSEEYLH
                     GPDPFIWNARDERGKGLPSGVYIIRLEWKGGSASSRIAVVQ"
BASE COUNT         2077 a         2397 c         2224 g         1929 t
ORIGIN      
        1 tttacgcctt tttacctcat cgagcttggc ccggtatagt ttcaaatagc ggattttgtc
       61 ctttacctgc ttctcaacca gttccatcag ggcttggcgg tcggaattca acacatcgat
      121 ctgtctggat ttgcctgccg tgtcatactg gaccatttta tcggcaagca gagattccct
      181 gccctccgcg tatacttcca aatccaacag acggtgcaca ttaagcgctt cataggcatt
      241 ctgatagtct tccagcgccg cgtagatctt gccgagcata aggtagtttt tcaggagcag
      301 ggccttgctc ttgatctggt ttgcgtagct catcgaagtc tgggtgtttt ccaacgcgct
      361 tacatagtct tccaggttat agtggcactc actaaggttg ttataggtga ccgccaggct
      421 ttcggggttg taaaaatcac tctttatctc caatgattcc cgatagatat ctacagcttt
      481 ccggtattgg gctttgtggt gataggtcgc gccgatgttg ttcagacttt tggcctgatt
      541 ctctctcatc ccgtattgcc ggaagtactc cagcgctctg agatgacaaa gctctgcctg
      601 gtccaggtct ttgagctcgc tgaaagtggc acccagattt gaaaggacaa tggcttccct
      661 ggccaggtct ccggcttcct gatacaagcg ttgcgcgtcc tggtaatagc ccagggcagt
      721 cgggtagtcc tgactcgtgt agaaaaggtt tcccaggttt agctttgccg tggccaggtc
      781 cgcggcgttc ccccatgcct ctttgaggtt cagggagcgg atgtaggctt ccatggccag
      841 ggatatctca ttggttttga tgtaggcatt ccccagattg ttgtagatac ttgacgtaag
      901 ccggtcactg acagtggaat cgcaggtgcg ggccgctctt tgataccagt ccaccgcttg
      961 ctgaaaatcc tgaagctccc aaaagacttg cccgatgtgg ctcaaactgc tgatctcatc
     1021 cgttctgcca tccagtctgc ccctgtaggt cagagactta tgcaaatact tcagggcggt
     1081 gggataatcc ttttctctgg taaagactct gcccaggttg tagttgatat cgcctttgag
     1141 cttggggttt tccacctttc tggcttgcgc cagggccgca agatagcact ctttggcctc
     1201 gctggtggcg ccccgcagaa aatggacatt tcccttcact ttctgagcgg cggccagata
     1261 agagggcagt ttgtgcaatt tggcctgttc cgctatttct gatatcagga tcaaagcctc
     1321 ttcgggatcc cgctgttgta gttcggtggc ttttagcagc aaatcctgca ggctggcctc
     1381 agggttttca gagacaaaaa ctcccgcttg ctcttctgta atcatggtac ctcatagtgt
     1441 tacggcatta tgctcaaagc tacaatcaca aaagctataa atatatttga tccctttttt
     1501 gtcaacttgt tattatcaaa tccggcaaat tcgggccaga ccaatcctgc tcagacagcg
     1561 cggtttgcag aaacttggcc tgattgaact ccgcgggaaa aaacatcttg acatataagg
     1621 ctttcgagcg gcagttgaaa aaagcgcaaa actcaaggct acccaaagcc cggaagcttt
     1681 ttgaagaggg agttgagcgc ctgccggagc tttcggcaga aatactggga gaacgactta
     1741 tgaaagcgac acacatagaa gtgggcaacc tctccgatct cggagtgaac cccgcccgcg
     1801 aaaagaatga ggactatttt ggccgctttg acggcgagtt tggcaccctg ctgatcgttt
     1861 gcgacggcat gggcggtcac attggaggcg aaatcgccag ccgcctggct gtggaagcca
     1921 tccagaacta tatcgccaaa taccacatcc ccaccgagga aaacatgatc atcgcccaga
     1981 gcatagaaca tgcccagcaa aagatcatgg aagcggtaaa tgatgatccc tctctggctg
     2041 gaatgggcag caccctggtg atgctgttga ttcgcgaaca ccagttctgg tatgcccatc
     2101 tgggagacag ccgtctctat ctgaggcgcg acgatgccat caccaggctc acccgtgacc
     2161 acagcgaagt gcagaacctt gtggacctcg gtgtgatcaa acctgaagac gcgcgcactc
     2221 atccccgccg cagcgtgctc agcaaagctt tgggacacga taccggcgat cccgagatct
     2281 caggacccca catgctttat caggacgacg tcttcctgct ctgcacggac gggctcaccg
     2341 aatatctgga agaccacgaa ctgctggatt acatgacgga aagccctcag gtggcagccc
     2401 acaacctggt ggaggaagcc aaacgccgcg gcgggatgga caacatcacc gtccaggtga
     2461 tcagagtcct ccacggcacc cataaaggca ggacggaagc tcccgccgag gtttccaacc
     2521 gcaagccggt gaattataag aaatacctgg tcccagccct ggctgtaatc ctcggcctct
     2581 atatcctggc catgatcccc aggacctgca ggaaggtctt tgaccgcaaa ccgatgacgc
     2641 atgaggattc actccgggtg gcagaaaaag caaggatcaa gcaggaaaag gcggaacaga
     2701 aacgtctggc caaagaggca aagcaagcca ggaaacagga taaagacgct cccgcggccc
     2761 agccaagcgc tcaggtagat cccgctttgg acagcgctct ggccgccaga ctgagtgcca
     2821 aaccagcaag tgccgactat cagaagttcc tggacgagct cagggccgcc aatcccagcg
     2881 ccaaacttcc tgccaaaatc agctttatct ctgatcagac cgatggacgc agcgttctgg
     2941 tgactccagg ccagacgata tacttcgcat ggaactactt ggacaagggt aacaaattga
     3001 atatggagca gttacagtat cttatcacga ttgccgccgc catgggtgat gcctcaatcg
     3061 caaaaagcgc tccggccaac gcggaaggct atttcagcgg tagccccagc gtgatcgatg
     3121 aggcaaccct cgccaatgcc agaaaactct gggtagccgc aaaacccgct ggagattatg
     3181 actttacccg cataggccgg tctgtcaaca agaacgcccg aaaatccggc tacacgctca
     3241 tctacaccaa gcctgccgct caatagcaag tctatatatt actaacatgt acaagtccca
     3301 tacctacaca ggaggatcga tgcggaaagc tctaatgggg atcctggttt tggctatcgc
     3361 gctgccctgc ctggccagcg tggaggtgaa gagcgtccaa tcccgcgatg ccggaaaaga
     3421 agccaacaac gtcaatatcg gcctgagtgg agacgcggct taccaggtgc agagttccca
     3481 cgatggcaag gtgcacaaaa tcatcgtgcg tgatgcccaa aatgccgtgg cccggcctga
     3541 ctatcaacgc ctgagcccag tgatcgatcg catcagaact taccgggaag gctccaacac
     3601 cgtcgtcgag atcaacacta tgcagcccag ccggatcagc cactcgcaat caagcgacag
     3661 gatatctctg cggctcgatc agggccagga aatccccccc gaacctgagg attccactcc
     3721 tctcgcgcct caaactcccg atctcagtgt ggaccgcgat cctgagcttc caaccaaggc
     3781 cgcggcaagg gtcaatccca ccaagctgcc ggcccccact caggcaagca ccccggagcc
     3841 cgaaaaaccc atctttcctg ccaccatcga tctggattca gcacccccac ctgctgagga
     3901 gcgtgaggag tctgcccagg cccagccagc cgagcaccct atcaaagaat ttctgcggat
     3961 ccactggagc tggatgctgc tgctggcaac gggactggcg atgttgatct atgccgtgag
     4021 ggcttgtctt agggccaaag ccacccatcg tcccaccaat ctggaacccg aagaaactcc
     4081 cgccctgatc atggattccg ctacccggca acgcatggtg atgaggctgg ctgaacaagg
     4141 ctggacggcc tctcaggtgg ccgcggagct caaattgccg gtcagggagg tggagcggat
     4201 catctccaaa tccaagcagc gcccggaact gtaaaaataa tgccgcgccc ccggacctgt
     4261 ctaattttgc ttggtctgct ggttttatcc tgcggattgg atgcttcggg caccttctcc
     4321 ttcgcgcgcg tcagcgtacc ctcgatgttt gatccccagg tgtcttccca atttgacgac
     4381 aactacctgc gctatcccta cttcctgtat tacgacgcac ccctcaaagc cccccaggga
     4441 gtcagtcacc ccttttccag tttcaagcgg ctgggagact ccatcgtcga tgccttcaat
     4501 ccagccaatc gtcgttatgc cctcctgcaa tacgcctatc gggacagtgg catggttttc
     4561 gcccctccgg aacttgccct taatggagtc ccccagagta aagacgcccc ccgggttggc
     4621 agcatgaatt ttcgcagcga gctcaacttc gtggccggtt acgaccagcg ctgggaagat
     4681 gacacccgct atggcttcat ctggaagggc atcgccctca aagccaatat caacaaacac
     4741 attgcagcca aagccatctg gaccaacgga gccttcaccg gagacagaga cgccgcggaa
     4801 gcgctttccc cccttgtgga tggctatatc acccacacgg cggagcagac ccggctggac
     4861 aacctcactg cccggctctc atactaccac cccaacctgg gtgtggaact gggccgcaaa
     4921 agccacaaca tcggcaacaa tctcagcggc tccatcgtgc tcagcgatca ggtgaacgac
     4981 tatggctatc tgaaagcgga agccatgttt ggcaagctgc gggtctcgct gctgcacgct
     5041 tccctcaatg ccgacagcgc cagagcggaa gccaaactgg cagacctcga cctgccggat
     5101 aaatatctgg ccctgcacga gatagcctgg tatagcgact tgcacagatt ctatgccggt
     5161 gaggcggtgg tgtatggaaa ccgcgctccc gatatcaact acctcctgcc tcacaccttc
     5221 tggagggtca cggaacacaa ccagtgggat cgcgacaacg tgctgatcta cgcgggattc
     5281 tccaaaagca gctatctaag ccacagctat tggttcaccg ggctctttga cgagctcagc
     5341 tacggcaagc tcttcagcaa ctggtggggc aacaaatggg ccgtccagag cggcgcggag
     5401 atctattttc cagccctcga gctgatttcc ctgcctccca aactggccgt ggaagtagtg
     5461 gccgtgagac cctggaccta tacccactat caaaaccaca ccatgtattc ccacgataaa
     5521 aagcccctcg gctatcccaa aggctccaac ctggtgagtg gcaccgcgga actggactat
     5581 cccctcagca tgaacttcca tctggtgggc caggcgtcgt ttacccgtca gggttccgag
     5641 ggtaacgact ggcgtctgaa ctactcggac tactttcccc ccgccctcat cgacacagcc
     5701 agaactccct ggtttgccgg ccagcggacc gacaccctgg agctgggcag cctcctcaag
     5761 atctatctgg cccatcacaa cttatacatc ggccaccgcg ccaaaaagac ggacaagtgg
     5821 gaacacagcg tcttcgcggg atggctgttt ctgtattagg tagggttgtg atgctcaaac
     5881 tatcatccag gcagcgcaaa caaggacgtt tgcggttccg ttattgatat gcccatagtc
     5941 gatcgcctcc ccttcccacg taaatacaag ctccatcagg gcgtggtgct gctggtcatt
     6001 gtggccgcgc tgtttggatt atggctgcgc tcccaatcac gcaaagccct gggagagaga
     6061 gtgatcatcc actccatcgg catcgagcgc tatgacaccc agtttgtgga gatcaacttt
     6121 gagatcgaaa acaagggcaa gcgagcggag gacgtgatgc tgctggcccg gatctatgat
     6181 cccgctggag acgagatcgc cagcaaactc ttccagatcg ctctgaagcc atcctggaaa
     6241 ggcccccggt ctcacatcct ggacaaaatg acccgccctc tgaagcttgg ggaaaagcct
     6301 tatcgggcca ctatggagct gcgaactcga taggctgtgt ctttattaaa acagaggggc
     6361 agagggacag agaattttga gggacagagg attgactatt taaaaatgca ggttttagga
     6421 gccggaaacg tcctcgtttc cgtggtctgt agcgtcgaat tccaattctg cgcatatcaa
     6481 accgcgtaac gctgaacgca taacgcccta aaacacagag aaaagcagag ggatttcaga
     6541 gaaaagcaga gggatttcag agaaaagcag aaagaggttt ataacgagta aaagaacaaa
     6601 gagcgtacat ttatctttgt tcgcaacgca atgcatccat gggcgacagc ataacgcgta
     6661 acgcgtaacg cataacgcgt aacgcataac gcataacgca taaccctaca aatcaatcta
     6721 ccccttgaat ccatagcaac acctattgaa ccaccgcgat tctggagctg gcgctgcctc
     6781 ccttccattc cagcctgatg atataaacac cactgggcag gccttttcct ctttcgtcac
     6841 gggcattcca gataaaggga tcggggccat ggaggtattc ctcactctta agcatctgcc
     6901 ctttaatgtt ataggttttc acggataccc gggtttggac tgccagatca agacggacct
     6961 gagaataacc cctggacgga tttggccaca tagccagcct gacaaccgga aggagtagct
     7021 catcatcaac ctccacctgg ctggtgtgag agtatttaat gacatatcca ggcacgtcag
     7081 aataaccatt ggggctgcaa agattggtgg gcaatccatg ccaatcccag gcccaataac
     7141 tggcacccag gtatagataa taacctgttt ccatctcatg cagcacataa ggtatgggat
     7201 agtcaccctc ccattcatta ttggaccaat agctggaaga gatggtgcct atcttccagg
     7261 cgttattgat gtagagtggt tttgtggtgc cactaatggc gttgcgtaag ggcgggtagc
     7321 tcatatactt ttcaaaatca gcctccaggc ctgaaggctc cccagtgtaa gagtaacata
     7381 cccgcgagga gggtacccag atgagggaat ccgcggagct gtaaatcact tcctggaggc
     7441 gcctgcccag actgtccact tcgcaatcca gcctggacca tgaagacagg tttcgtttgt
     7501 agatggcctg ggttagtttc atatcatcgt tgtattgata ccaaatcctc atcacatgat
     7561 cggtattggc gatgtcgttt gaatagtcgt cctgcagata gtagccgtcg ttagtaatcc
     7621 tgctgctgcg gtagggaggg actgtcccgc tggattctcc ccctggacct ctgcaaatga
     7681 aatagccatc gtgttgttca aaactgccgc caaagacaaa ggtggtatcc gtggtagtgg
     7741 tctcgccatc gcaggtgtag gaataaaccc ttctgattga gttgaccacc aggggattat
     7801 cctgggcgtg agcgtagaag taatgataac tggagctgga gccgggacgc attccggagt
     7861 aaatgtgcat tgtgaactca ttgatataga atacttccgg gacgggttgg gcaattaagg
     7921 agcccggatg gactagcggg aaagccatca gtagggcaac aataaagctc gggtaaactg
     7981 atagggtgag tattcttctc atcaaaactc cctggcctgt ggtctcaaaa gctgtttgta
     8041 tctgattgtc actgcgggac tggcaatatt gtctaataat gacgtcaggt caagaatccg
     8101 cgctgctaac aagctgtcaa tgcttttttt ggccttgttt gtcagtctgg ttagaaggaa
     8161 tccacttgct tgctggcgct caggatagtt ttgctaagcc tgattctgca gagaggtctg
     8221 cagagacgac gccaggctag ccacccagtt cgcgcttaat cacccgccgt ggagataccg
     8281 tggggcctcc gccgtttttc gccggaaagc gcgccgcatc tctacggcat gtgaataggg
     8341 aaggatcagg caatgtccca ggtctggcgt gctggtctgt aaaagacatt tgcacatagt
     8401 aagagcgttg aatgattggt atcactggct tcagagcttg tacgagaaag ccgtcctgga
     8461 gttcaaagcc cctggccaga ataggcgttt atatgcttgt ttgacagggc tttggactta
     8521 cgtgcaggat atcggagcaa taattttccg tgctggttga agtccaagcg gtccgcaaac
     8581 tcgttgtcat gtatgccttt atcgtgaccg cttgaactcc aacgggt
//
LOCUS       DAOSFN010000111         8608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_6992_length_8608_cov_9.916754, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000111 DAOSFN010000000
VERSION     DAOSFN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8608
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6992_length_8608_cov_9.916754"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PLO35_07410"
     CDS             complement(<1..1340)
                     /locus_tag="PLO35_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79534.1"
                     /translation="MAKTQLNLELSYKGKILDYIKNGSGVFKDKWFVGSNKYLLWQIL
                     DPAFPDKHQLIAQKGNDFFMKLVPGTKLECSKAGKQVDQAFLSSNGLLKGDQLQLQND
                     MSGTLVLNPDWQIHYRFIEPYVHILTEEERQIVAQYSRRAESSAEEKLGRGMILVFLF
                     LTLALLVLYDLVLKPDAVDAATLDNTLAQLRKAQRVDMPDRSQDEADMQGDEAAEKAR
                     REEEARKAAEAAAAAAAAAGTETNRPQRGTTTGTPGGTGTNAAALFGEFNPNAVVRNP
                     VAITTTRDFVAASRGGTGGTGGSGGTGGPDGSGGSGTSGGMRSTYDPNAIASYQQSDF
                     SSASNTGGIKGSSVKPTGPVEVKGNLNQAQIAALGRPVALTSTDKAVINRISKEAPRV
                     TENTIAQAPEGQQTDLQALRAVVNSRKGQLTSLYRKTAAIENSGGAVTIRIFGRG"
     gene            complement(1350..1895)
                     /locus_tag="PLO35_07415"
     CDS             complement(1350..1895)
                     /locus_tag="PLO35_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOH79535.1"
                     /translation="MKIIDKAKSTGREVRRTRKIAADIKPLSITSLLDVLTIVLAFMI
                     KNVSMEAQKITIPERMVFPTTMSADQLEESMGTTVVKVYPDRILLGLESAYFGTPLQL
                     LTDVQKRKDLNEYLELQSNQIEKHADKNQACLLVQADEVITTEVITAIVEVGTGSYYK
                     YIYFSTLKDPQWLKNSQLARQ"
     gene            complement(1904..2425)
                     /locus_tag="PLO35_07420"
     CDS             complement(1904..2425)
                     /locus_tag="PLO35_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOH79536.1"
                     /translation="MPYRPSAGRNQRLAGGPKEPDLVPIMNLFLTIIPFMLYMLVITQ
                     LALIALNFAPSGGGGGGGQGPGGDAKAEEKKIEVYIMNQDAPDRGLIRGFEVREPGME
                     NIKVPFVNGNYDFVRLDQILKDIRARNPEQREINVSPYPDVLYGPLIQTIDLSKSNGF
                     INVMFKPTGVGYI"
     gene            complement(2430..3182)
                     /locus_tag="PLO35_07425"
     CDS             complement(2430..3182)
                     /locus_tag="PLO35_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOH79537.1"
                     /translation="MKAKHIVLVVMVLLFCAALIAQEAQQEVKARGVSLKTIFEDSRE
                     WGYAIILTFVIGLVYGVVRYLQLYQREKIDAQKFYLKLKGYIKNDQVDEAAKIAESFK
                     GTTLGYIFWSGLRIYIDTRKANKKGEELDRAVQNGFEEAVLQTVHKLDANLSWFTTLA
                     QICTYLGLLGTIWGLLVAFDALATAPENQKNYVLTAGIAQAIGTTAMGLLAAIPLTLI
                     HGGLFGRAQKILAEIDEHTVKIINYLSNANRG"
     assembly_gap    3232..3331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3332..3634)
                     /locus_tag="PLO35_07430"
     CDS             complement(<3332..3634)
                     /locus_tag="PLO35_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79538.1"
                     /translation="MKRYIAIAIILLGTGLLLAQTTTPNPTAPPSGFENVRRLTGDTF
                     TVEIKALIPDITTFKNKAETYNKEGYYNFPKDFLGLVQKLSKEQITNWQVIRNKWLE"
     gene            complement(3640..>8608)
                     /locus_tag="PLO35_07435"
     CDS             complement(3640..>8608)
                     /locus_tag="PLO35_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH79539.1"
                     /translation="MDKVFESKYQKFVNEDPEILGKDFSDMELKRLLDIEQNLKLYQG
                     LVAEIETTMATPEYKNQSSFDLSGELKDYREKIQDLQIRKDRYISTRKHESDFEEQLF
                     QEFLNEYYALNQESDELLLTIADIEDYMISSMMMGYPDEMKKIAQAHREKIKGQQEFG
                     GLDAQLAMVSANLDYLSLQNRYRGLNYQDAKRKADTSLSIEETTRQYNQIIDQKKVLI
                     NDYQTFLTSYPEFVAFEQPSDLYEPSDSLLISSADIYYNLAELQWSTDLDSPDKALAY
                     YRKVLELDPDFYLKDYALYNVAYFSSEINREQKQRYIDNWRTLNPNKQRGVEQRLTEV
                     DFSEALANYNDIITNYPDSPLYDEATYRLANLYFIIGTDAERPIEWYARANSLFDRLA
                     AKENSPYRYEAMFQRGFVNMNISDDSSLNAALVDFANILNAVDNNAITPAETAQDLKN
                     NSLDQIAFCLVALDGSDFEGESKGLQALASVMADYQDEKALTRILDKASENKKDMRLT
                     RQSIDFLELRLQKSPVALINPSLVDSILVLYTSNDTVLRAGEDRDQIRRDKYRLMTEN
                     YGKDSRWYEQNVKDKDLTDPAIIKQLASIRNAYRQAQIVKYEAVRTQLTEESYQAYLD
                     HNVKFAAYEELFAPSVYTAYADHLTRYFKATPSAYDADINEWKRNNEQLDLLLLGNLA
                     DKRQTDEAYMMVYNRLLAYNEKYLVPASPEYFDNEELAYGYAIRRDQLLKPEAQGAER
                     DSLYAFYKAATLRFVDTLMKSGTEARIARARQLNLDLANIEFNDNRFAEAEARYLNIL
                     DNDPSLSNDGKFDIYYNLSFVAESDTSYAPYDRYKRSESYARQAMAYTANGEKIALAT
                     RQVQLQIQNSYNAATASNDYAKAAEEYGRMALEFPYDKYPAEHLNYKRLQAEQYQLAK
                     DYNNLIDTYMYLASKEKAENVDAVFGLYYLSWTTADSLMQDPARAASIKGEFMAKYPA
                     SFQTYSLRAQDIEKKAADPATRIAAAEDYIALSEEVRTGKIDAGEVKPENIYMQAYNI
                     YNEDQNTAKRLEILDEFTKRYPSDPDVTGMLKTIASGYFTLGDTLRFESYARQLFQRD
                     NKEYDLYQRVAVKYLARILAEYDSAYLNKDWTLAFQKRDQFKAEEAKYRKEGLPLDTT
                     DQYKAFAAAEEEYKNIQAQIAFLKNFDTQVTALERSAFLGKNPNQQFGVGAKTSFKST
                     LLGGKSKLLPTFINTVNAEVAKVERLITPANQLKLDAPRIVRALALEGRIYEYSIGVI
                     NSQINRFFEQANEIRPYKDDPNVQANIWGQINNDFIYPLEDNANAVYGAIFTTFHMAG
                     YEDQNTQQAVAKLTERKLMPDYQKLEFPLGSSGWELSWISDDGTQTAATPVVGTITTP
                     KGQVMGNLQIPPVTKLQAEFKLSSKIAPDLVYLQMIYPYNPEAMVNGKEVQFAGFAVD
                     SLDVKQPMTARFGYKFSGAEWDAKENTIRLLFPNRYQDSIPVRATLQAYFDKAKLEQA
                     RPRETVTFSSGPSWKVMVLDPETQVENAIPAMVVENYGIPQEAINGFKNSQAQPVWIL
                     ETPEAPVNLVVFEADFDLENVPISAQIEFLAPDMASVFINGNLVAADMPLDGEMEPLS
                     VYTTITDLPLDFLRSGKNTIRFEVRNQSAYRGMLAEIKIERYTKE"
BASE COUNT         1773 a         2106 c         2275 g         2354 t
ORIGIN      
        1 ccgcggccga agattctgat ggtgaccgcg ccaccactgt tttcgatggc ggcagtttta
       61 cgatacaggc tggtaagctg gcccttgcgg ctgttgacaa cggcacgcag ggcttgcaga
      121 tcggtctgtt ggccttccgg ggcctgggca atggtgtttt ccgtgacccg gggagcctct
      181 ttactgatcc tgttgattac agctttgtcg gttgatgtga gggcgacagg tcttccaaga
      241 gcagcgatct gggcctggtt gagattacct ttcacttcca cgggaccggt aggcttcaca
      301 gagctgcctt tgattcctcc ggtattggag gcggatgaga aatcgctctg ttgatagctg
      361 gctatggcat tgggatcata agtggatctc attccacctg aggtacccga gccaccgctt
      421 ccgtctggtc cacccgtacc acctgaaccg ccagtacctc cagtaccgcc acggctggct
      481 gcgacaaaat cacgggtggt ggtaatcgct acgggattcc tgacaacggc gttgggattg
      541 aactcgccaa acaaggccgc ggcattggtg ccggtccctc caggggtacc agtggtagtg
      601 ccacgctggg gacgatttgt ctcagtgcct gcggcagcag cggcagcagc ggctgcttcg
      661 gccgcttttc tggcctcttc ttctctgcgg gccttttcag cggcttcatc cccttgcata
      721 tcggcctcat cttgggacct atcaggcata tccactcttt gagcttttct gagctgggcc
      781 agagtgttat ccagggtagc ggcgtccact gcgtccggct tcaggacaag gtcatagagc
      841 accagcaagg ccagggtgag aaacagaaat accaggatca tgcccctgcc cagtttttcc
      901 tctgccgagc tttcagcccg tctggaatac tgggccacga tttgcctttc ttcctcggtg
      961 aggatgtgca cgtagggctc tatgaagcga tagtggatct gccagtctgg attgagcacc
     1021 agagtcccgc tcatatcatt ttgcagctgg agctggtccc ctttgaggag gccgttgctg
     1081 ctcagaaagg cctgatcaac ctgcttgccg gctttggagc attccagttt ggtgccgggc
     1141 accagtttca taaagaaatc gttgcctttt tgcgcgatca gctggtgttt atcaggaaag
     1201 gcgggatcga ggatctgcca cagcagatac ttgtttgaac ccacgaacca tttgtcttta
     1261 aaaacacctg aaccgttttt gatatagtcc aggatcttgc ccttgtagct aagctctagg
     1321 ttaagttgcg tctttgccat atctcctccc tattgccttg ctaattggga attctttagc
     1381 cattggggat ccttgagggt gctaaagtag atatacttgt agtaagaacc cgtcccgacc
     1441 tccacaatgg ccgtaatcac ttccgtggta atgacctcat ccgcctgtac caacaaacaa
     1501 gcctggtttt tatcggcgtg cttctcgatc tggttggatt gtaactcgag atactcgttg
     1561 aggtctttac gtttttgaac atccgtgagc agctgcagcg gcgtaccgaa gtaagcgctc
     1621 tccagcccca gcaggatcct gtcaggatag actttcacca cggttgtccc catgctttct
     1681 tccaattggt cggcagacat ggtggtggga aagaccatcc tttccgggat ggtgatcttt
     1741 tgggcttcca ttgatacgtt tttgatcatg aaagccagca caatggtcaa cacgtcaagc
     1801 agggatgtga tggaaagagg tttgatatca gccgcaatct tgcgggtgcg tctcacttct
     1861 ctaccggtgg atttggcttt atcgataatc ttcatagtgt ctcctaaata tagccaacgc
     1921 cggtgggttt aaacatcacg ttgataaaac cgttgctttt agacaaatcg atggtttgga
     1981 tgagggggcc atacagaacg tccggataag gactcacgtt gatttctctc tgctcaggat
     2041 tgcgagcgcg gatatctttg agtatctgat ccagtctcac gaagtcatag ttgccattca
     2101 cgaatgggac cttgatgttt tccattcctg gttcgcggac ctcaaatccg cggatcagac
     2161 cccggtccgg agcatcctga ttcatgatgt agacttcgat cttcttttcc tcggctttgg
     2221 cgtcgccacc gggaccttga cctccccctc ctccaccacc ggacggagcg aaatttaaag
     2281 caatcaatgc cagctgtgta atcaccaaca tatagagcat gaatggaatg atggtgagaa
     2341 acagattcat gatcggcacg aggtccggtt ctttgggacc cccagccagc ctttgattcc
     2401 ttccggcaga aggacggtat ggcataatac tatcctctgt tcgcgttgct aaggtagttt
     2461 atgatcttaa cggtgtgttc gtcgatctca gcaaggatct tttgggcgcg gccaaacagg
     2521 ccaccatgga tcaaagtgag tggaatggcg gctaaaaggc ccatggcagt ggtcccgatc
     2581 gcctgagcga taccggctgt cagcacatag tttttctgat tttctggggc cgtagcaaga
     2641 gcgtcgaagg cgaccagcag tccccaaatc gttcccagca gtccaagata ggtgcagatc
     2701 tgggccagag tggtgaacca ggacaggttg gcatcgagtt tgtggacagt ctgcagcacc
     2761 gcttcttcaa atccgttctg cacggcgcga tccagttctt cacctttctt gttggcttta
     2821 cgggtgtcga tgtagatgcg cagcccgctc cagaagatgt aacccagagt ggtacccttg
     2881 aaactttcag cgatcttggc ggcttcgtcc acctgatcgt ttttgatata gccctttagt
     2941 ttcagataga acttctgcgc gtcgatcttc tcacgttgat agagctgaag gtagcgcacg
     3001 actccgtaaa cgagtcctat aacgaaagtg agaataatgg cataacccca ttctctacta
     3061 tcttcaaaga tagtcttgag agaaacgcca cgagctttga cttcctgttg agcttcctgc
     3121 gcgattaagg cagcgcagaa tagcaatacc attactacca gaacaatgtg tttagctttc
     3181 attttgactc ctctagccat ttatttctta ttacttgcca gtttgtgatc tnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctctagcca tttatttctt attacttgcc
     3361 agtttgtgat ctgttctttg cttaacttct ggaccagacc cagaaaatcc ttggggaagt
     3421 tgtagtaacc ttctttatta taggtttccg ctttgttttt gaaagtggtg atatcgggga
     3481 tcaaggcttt gatctcaact gtaaaggtat ccccggtcaa tcttctcacg ttttcaaagc
     3541 cactgggggg agcggtggga tttggggttg ttgtttgggc aaggagcagg cccgtgccca
     3601 gaaggatgat tgcgattgct atatatctct tcattggtct cactccttgg tgtacctttc
     3661 gatcttgatt tctgcgagca tgccacggta ggcggattga ttgcgaactt caaatctgat
     3721 agtgtttttg cctgaacgca ggaagtccag aggcaggtct gtgatggttg tatatacgct
     3781 cagcggctcc atctctccat cgagaggcat atcggcagcc accaggtttc cattgatgaa
     3841 cacactggcc atatctggcg ccaggaattc aatctgggca ctgatcggca cattttcaag
     3901 gtcgaaatcc gcttcaaaga ccaccagatt taccggcgct tccggggttt ccagaatcca
     3961 gacaggttga gcctgactgt ttttgaagcc attgatggct tcctgaggaa tcccgtagtt
     4021 ctctacaacc atggcaggga tggcattctc tacctgggtt tcaggatcga gtaccatcac
     4081 tttccacgat ggcccgctgc taaaggtcac agtctcacgc ggacgggctt gctccagctt
     4141 ggctttgtcg aaataggcct gcagggtagc cctgaccgga atactgtcct gataacggtt
     4201 ggggaagagc agccggatgg tattttcttt ggcatcccat tccgctccgc tgaacttgta
     4261 tccaaaccgg gcagtcatgg gctgtttcac atccagagag tccaccgcga atccggcaaa
     4321 ttggacttcc ttgccattga ccatagcttc aggattgtag gggtagatca tctgcagata
     4381 gaccagatcc ggagcgattt tggaactgag cttgaattcg gcctggagtt tggtgacggg
     4441 aggaatctgg agattgccca ttacctgacc tttgggggtt gtgatcgttc ccactacagg
     4501 cgtggcggct gtctgggtgc catcgtcgct gatccagcta agctcccaac cgctgctgcc
     4561 cagcgggaat tccagcttct ggtaatccgg catgagcttg cgttctgtga gtttggctac
     4621 cgcctgctgc gtgttttgat cttcataacc ggccatgtgg aaggtggtga agatagcccc
     4681 atatactgca ttggcattgt cttccagagg atagatgaag tcgttgttga tctggcccca
     4741 gatattggcc tggacgttgg gatcatcctt gtatggcctg atctcgttgg cttgctcaaa
     4801 gaaccggttg atctggctgt tgattactcc gatcgagtat tcgtagatgc ggccttcgag
     4861 agcgagggcc cgcactattc ttggagcatc cagcttgagc tggttggcag gggtgataag
     4921 ccgctccacc tttgccactt cggcattcac ggtgttgatg aaggtgggca gcagttttga
     4981 ttttccaccc agaagggtgc ttttgaagct ggtcttagct cctactccga actgctgatt
     5041 ggggtttttg cccagaaaag cgctgcgctc cagagcggtg acctgggtgt cgaagttctt
     5101 caggaaagca atctgcgcct ggatgttctt atattcttcc tcggcagcgg cgaaggcctt
     5161 atactggtcc gtggtatcga gcggcaagcc ttccttgcgg tacttggcct cttcggcctt
     5221 aaactggtca cgtttttgga aggcgagggt ccagtccttg ttcagataag cgctgtcata
     5281 ttcagccagg atgcgggcca agtatttgac ggcaactctc tgatagagat catattcttt
     5341 gttgtcgcgc tggaaaagct gcctggcata gctttcaaac ctgagggtgt cccccagcgt
     5401 aaagtatcca ctggctatgg ttttcagcat gccggtcacg tctggatcgc tgggataacg
     5461 ctttgtaaac tcatccagta tctccaggcg ctttgctgtg ttttggtcct cattatagat
     5521 gttataggcc tgcatgtaga tgttttcagg cttcacttcc ccggcgtcga tcttgccggt
     5581 cctcacttct tccgagaggg caatgtagtc ttcggcagcg gcgattctcg tagcggggtc
     5641 agcggccttt ttttctatat cttgcgctct gagagaatag gtctgaaagc tggcaggata
     5701 cttggccatg aattcgcctt tgatcgaggc agctctggca ggatcctgca tcaggctgtc
     5761 tgccgtagtc cagctgaggt agtaaagacc aaatacggcg tcgacattct cggctttttc
     5821 tttgctggcc agatacatgt aggtatcaat cagattgttg tagtccttgg caagctgata
     5881 ttgctctgcc tgtaatctct tatagttgag atgctccgcc ggatacttat cataggggaa
     5941 ttccaaagcc atgcggccat attcttcagc ggctttggca tagtcattgg atgcggtggc
     6001 agcattatag ctgttttgga tctgcagctg tacctgccgg gtggcaagag caatcttttc
     6061 cccatttgcg gtatatgcca tagcttgcct ggcgtagctc tcagatctct tataacgatc
     6121 gtagggcgcg taactggtgt cgctttccgc gacaaaagag agattgtaat agatgtcgaa
     6181 tttgccgtcg ttgctgaggc tgggatcatt atccagaata ttaaggtatc tggcttcggc
     6241 ctcggcgaaa cggttgtcgt tgaattctat attggccaaa tccagattca gctggcgggc
     6301 cctggcaatc ctggcttcgg tgccggactt catcagagtg tccacaaatc tcagcgtggc
     6361 ggccttgtaa aacgcataga ggctgtctct ttccgctccc tgggcttcgg gtttgagaag
     6421 ctggtcgcgc ctgatggcat agccgtaagc caattcttcg ttatcaaaat actcgggact
     6481 ggcgggcacc agatacttct cgttgtaagc cagcagacgg ttgtaaacca tcatataggc
     6541 ctcatcggtc tggcgcttgt ctgccagatt gcccagcagc aacaggtcca gctgctcgtt
     6601 gttgcgtttc cactcattga tatccgcgtc ataagctgag ggagtggctt taaaatatct
     6661 ggtcagatgg tccgcgtagg ctgtataaac cgaaggagcg aagagctctt cgtaggcagc
     6721 gaatttgacg ttgtggtcaa ggtaggcctg atatgactct tccgtgagct gagtacggac
     6781 cgcttcgtat ttcacgatct gggcctggcg ataggcattg cggatggagg caagctgttt
     6841 gatgatagct ggatcggtga gatccttgtc cttcacattc tgctcgtacc agcggctgtc
     6901 tttgccatag ttctcagtca tcagacggta cttgtcgcgc cggatctggt ctcggtcttc
     6961 gccagcacgc agcactgtgt cattggaggt gtagagcacc agaatggaat ctaccaggct
     7021 gggattgatc agggccacgg gggacttctg cagtctcagt tccaggaagt cgatagactg
     7081 acgggtgagc cgcatatcct ttttgttttc ggaagccttg tccagaatgc gtgtcagggc
     7141 tttctcatcc tgataatccg ccatcacgga ggccagagct tgcagacctt tggattcacc
     7201 ctcaaaatca gatccatcca gggccacaag gcagaaggcg atctggtcca gagagttgtt
     7261 tttgaggtcc tgggcggtct cggcaggagt gatagcattg ttgtcgaccg cgttcagaat
     7321 attggcaaaa tccaccaaag ccgcgttcag cgagctatcg tctgagatgt tcatattgac
     7381 gaatccacgc tgaaacatcg cttcgtaacg gtaggggctg ttttccttgg cggccagcct
     7441 gtcaaacagg ctgttggccc tcgcgtacca ttcaatcggg cgttccgcgt ctgtgcctat
     7501 gatgaaataa aggttggcca ggcggtaagt tgcctcgtca tagaggggcg aatccgggta
     7561 atttgtaatg atatcgttgt agttggccag ggcttcactg aagtccacct cggtcaatct
     7621 ctgttccacg ccacgctgct tattggggtt cagagtacgc cagttgtcta tgtagcgctg
     7681 tttttgctcg cggttgatct cactgctgaa ataggctacg ttgtaaaggg cgtaatcctt
     7741 gagatagaaa tcagggtcca attccagcac tttgcgataa taggccaggg ctttatcagg
     7801 gctgtcaaga tcggttgacc attgcagttc cgccaggttg taatagatgt cggcggagct
     7861 gatcagcagg gagtcgctgg gctcatacag atcgctgggc tgttcgaagg ccacaaactc
     7921 cggataactg gtcaggaagg tctgatagtc gttgatcagc acctttttct ggtcgatgat
     7981 ctgattatac tgacgggtgg tctcttcgat agataaactt gtgtcggctt tacgtttggc
     8041 gtcctgatag ttcaggccgc ggtagcggtt ttgcaggctc aggtagtcga gattggcaga
     8101 aaccatggcc aattgcgcgt caagaccgcc aaactcctgc tggcctttga tcttttcgcg
     8161 atgggcctgg gcaatcttct tcatctcatc aggatagccc atcatcatgc tggagatcat
     8221 atagtcttca atatcggcaa tggtgagcaa cagctcatcg ctttcctggt tgagcgcata
     8281 gtactcgtta aggaattcct ggaatagctg ctcttcaaag tctgattcgt gcttacgggt
     8341 agatatataa cggtccttgc gaatctgaag atcctggatc ttttccctgt agtctttcag
     8401 ttcgccgctc aggtcgaaag agctttggtt cttatactca ggagtggcca tcgtggtttc
     8461 gatctcggcc actaagccct ggtagagctt gaggttctgc tcaatgtcca gtagccgctt
     8521 gagctccata tcgctaaagt cttttcccaa aatctcaggg tcctcgttta caaacttctg
     8581 atacttggat tcaaagacct tgtccatg
//
LOCUS       DAOSFN010000112         8523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_7107_length_8523_cov_9.661313, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000112 DAOSFN010000000
VERSION     DAOSFN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7107_length_8523_cov_9.661313"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..4736
                     /locus_tag="PLO35_07440"
     CDS             42..4736
                     /locus_tag="PLO35_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOH79540.1"
                     /translation="MNYDFGNVTVGETGTHSIVLTAQGPAGDSLHITTLHFTPDDDFG
                     FTSPYEGAMHLLSGETMTLDVGFFPVAEGPDSGILECEYTRNGESTLLTMEAPLYGTG
                     VAVAAPVLSVNPDEYDFGTDITPGETGSVSFNVTRNPDYAGVTSSVMEASLTGDASFS
                     LASSILDATGGTISLPYALDGSNMAVFTVDFEPWNTDPQHAVLKFVDNHGDSLSVEIS
                     AYSGLISVGPLIEVNPMNVVMTMSPSSVQSGVLSISNVGTDPLTYSIPAALQPPDVTV
                     WPNSGTIAAGDSSYVQYTVNSSSMVLGTQTRTITIESNDYGHSPTTVILSITVTEPPN
                     PVDFVGIPLNGHAALEVQFTDTSPIEAAGWRWDFENDGIIDNYTQNPLHTYAMPGVYS
                     VRLVTISSSGVYSERIKSGYVTVTNAAPIILPGAFTEQEFMEDQIGGPYGLSYVFSDP
                     DGDPLTYAVNGSDNIWGSVNPMAMLSLQATPNWSGTEIISVVAQDPFKGAVTHTFSVT
                     VLPVNDPPALTVPEHLYFIRNSEFEVDFSQYITDADNAMAQLSISLERIGGWIPLIFF
                     EYSPVNAANIPGQFSVIFTSQQQSEVTNGFRITVNDNAGRAVASADFQMHLLEHFNAS
                     VSTAELYQYTGQTVQFRDVTLGNPNYWHWDFEDDGIIDSNDPHPTHVYNSAGVYNVRL
                     TLGHSGAQEEDFIFLPGYITMYGTSVTDDDIPPTWTLQGSPYNLFGDIVIGAMDEIDI
                     EPNVEVNLFGNDPLLVNGVLNANQARFKPQNQNGRWGGLKFQGSNQREPSELIGCEIV
                     DALLPIDIEDASPLISGVTITVSDTTQYEDSRGIRIQGDSSPSLIDVELINYRQGVSI
                     QNDGNPDRDTPTLTNIRVRNSSQTSRDDLDPGTGIFISGAAVLNTVLVDNFGSAIVID
                     GTENNTTPTLTNIRVRNSSQTSWGDRYGIQIMGGAVPSLEDIDIEDVIYGIQVLGGGD
                     VQRDTPTLTNIRVRNSSQTSRSDTWGISIQDLSSIELEDAQFDGFDTGIRINAQTRVE
                     STPTLTNIRVRNSSQTSRQENIGIDISGSVTAQMDDVIVEDYPFGIIYSGSLSSRDVS
                     TPTLTNIRVRNSSQTSRNLAIGVQLSDLARVHCSDFIIEDYQIGLEISNDTSLRDVST
                     PTLTNIRVRNSSQTSRYENAGIFLGAGVGGTLTGAEVEEAIIGIFIADGNTTKVEPAR
                     IFNCESGIKAASSLLPKSLQRHLIVLEPAFLNEHFDWQFKAFDLEMPGPWLIENNTIN
                     GLTTYVKAEDAGILFINNIAWTTSPSMQPFQLQNSVLNASYNDMFVSPPYPGIGNLAL
                     YPEYVNPAEREFGITYDSPCIDTGSPLSPKDEDGSRADMGAFPYLHRASMVVSQRFVQ
                     PNTQVFFTNTSIGHDHLNSFCEWDLNKDGQAEATSRDWAHTFTEPGVYNLRLRMHSGN
                     LEDIRDYNAVVVVQSALLQGPPQVTIGRLANDIVLDWEPVTLTQQGDPVVVEYYLIYS
                     CEEPDGYFDFVGNTQNFETIYTHLGGAEADKRFYFVLGFTGTMRELQQYIELHRRLRP
                     SSMQTPLMRNKP"
     gene            4838..>8523
                     /locus_tag="PLO35_07445"
     CDS             4838..>8523
                     /locus_tag="PLO35_07445"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH79541.1"
                     /translation="MPQQRDFYAAILFLDVSGFTRITEAASARGHYGVELVTKVLNRY
                     FGRVHKLITPLGGEIVKFGGDACLIVIPIQPGACSEEHKPDIPALIAAILEEVSRLDR
                     RFRKEYGFPFSIHGAWGKGIVSLSVVGDSSRHLDYYVSSPDLKAVYDLAETSQAGQIQ
                     GPATHAQPPPELPDLPAKGSRAARLFLPSDIIRKLELEPRPAELRNATVLFIKLSPSS
                     SDDITLDQYQAAFHRIQSLITNHLGLINKIDFNEKGYLILALFGVPFVYGNDTLRAFS
                     SAYRISLLRLEGISIQIGITYSNIYCGIIGAPARHEYGIIGNAVNIAARLMSFAQPGE
                     VCLSKELIPHLEGQFETAWLARASVKGIAEPLDIYRLLRMLPARWGSLEEQYRADPLF
                     LDPLKLQTLQQGLASQGEFLCLIRGESGTGKSQLIFKLCEPWLNSEPPFQLIPAEPHM
                     RDLRLEIFFWSARKELGITNFREELGHILQWYGSQTAGPEPDLTEDLPDVHILSDLLF
                     GAPSSSSRQQIALDLILDILIRMYPQGRMLVLENFHNFDAQSRDLLLRLIRHKLYQGD
                     KIIVSSEATLPDELCQGFSFAGVCLENWDEELSGRYIRHRVPNITTRAIQLLHQISEG
                     NPRFLRGLTGHIQTHWSASQDLITHQIIESMRARGLLPNDLENLFRAEYEALNTSEQV
                     FTRLASIYGRPFRLSDFMQIFPTHSFSSLAGSADRLLQLGVLRHEAGSDAQVLWFVNP
                     LLPETVYRSILQSEKLAFHRSIATYYSSLQSQDELVWDLIAHHWLRAEDRPKIAWWCK
                     KLADHYYISGAFELSLRMWQQLVLWPLNEDAHTQAQLKCAELYLILADNDKAGRILES
                     YQHLAHQPAKHCESWIYLSARLMINRSQYSELDDFLAQHADRIQDPVIKDKLDTSHCE
                     AILQSMDPARIEAKALPLWNRLKEQERHLSLNSLAGIIGSYYINRGNYQTAASYYKEK
                     IKLAKRLKDPVSMRIGLSGLGIALSRMGQKTKALEHYQQALALASKSGDRNGFSKALL
                     DLGVYHRNEGDYPKALQYYQQSLSLAEYIGNKLQISIVIYDLGELHYYQQNWDEAESY
                     ISKSLELAKQLGDQVGMSFCYDALGDINFQRKKFPEALAIYRANLLVQHRLNDMEGKA
                     HTLGNLGNLAKSEKNYQRATKLYGSQITILQKVGDIDGEGRAWFNLAMLDFEQQNKEA
                     ARPKLTKALELFESCNARYYIDITR"
BASE COUNT         2000 a         2542 c         2184 g         1797 t
ORIGIN      
        1 aggtaccgct gaccgctttc cggggcctgg tacctgctcc cctgaactat gactttggga
       61 atgtgacggt gggagaaacc ggaactcaca gtattgtact tacagcgcag ggaccggcag
      121 gtgattcttt gcatatcacc accctgcatt tcaccccgga cgacgatttt ggcttcacca
      181 gcccctacga aggagcaatg catcttttaa gcggtgagac catgactctg gatgtgggct
      241 tctttcccgt ggcggaagga ccagactctg gtattctgga atgtgaatac acccgcaacg
      301 gcgaatctac tctcttaacg atggaagctc ccttgtatgg aacaggtgtg gcagtcgcgg
      361 cacccgtcct gagcgtcaat cctgatgaat atgactttgg aactgacatc actccaggag
      421 agactggctc tgtcagcttc aacgtgaccc gcaatcctga ctacgccggt gtaacctcgt
      481 ccgttatgga agccagcctg acaggcgatg ccagcttttc gctggcgagc agtattttgg
      541 acgccacagg aggcaccatt tccctgccct atgctttgga tgggagcaat atggcggtct
      601 tcacggtgga ttttgaaccc tggaacacgg atcctcagca cgctgtgctc aagtttgtgg
      661 acaaccacgg agattctctg tcggtggaaa tcagtgccta ttccggcttg atatcagtgg
      721 ggcctctgat cgaagtgaac cctatgaacg tggtgatgac catgagcccc agctccgtcc
      781 agagcggggt gctcagcatc tccaacgtgg gaactgaccc tctcacctac agcatccctg
      841 ctgctttgca acctcccgat gtgacggtct ggccaaactc cggcaccatc gcggcagggg
      901 atagctccta tgtgcaatat acggtgaaca gcagttccat ggtgctgggc acccagaccc
      961 gcacgatcac gatcgaaagc aacgattacg gtcactcgcc caccactgtg atcctcagta
     1021 tcacggtaac agagcctccc aatcccgttg actttgtggg tatacctttg aacggacacg
     1081 ccgctttgga agtgcagttt accgatacat cgcctatcga ggccgcgggt tggcgctggg
     1141 attttgaaaa cgatgggatc atcgacaact atacccaaaa ccctctccac acctacgcca
     1201 tgcccggagt ctattccgtg aggttggtga ccatcagttc cagcggtgtc tatagcgaaa
     1261 ggatcaaatc cggctatgtg accgtcacca acgcggcgcc catcattctg ccgggggcct
     1321 ttacagaaca ggagtttatg gaagaccaga tcggcgggcc ctatgggctc agctatgtgt
     1381 ttagcgaccc ggatggcgat cctctgacct atgctgtcaa tggcagcgac aacatctggg
     1441 gcagcgtgaa ccccatggct atgctgagcc tgcaggcaac ccccaactgg tctggcaccg
     1501 agatcatcag cgtcgtcgct caggacccct ttaagggagc cgtaacgcat acattcagtg
     1561 tgacagtact gccagtcaat gatccccctg ccctcacggt gcctgagcat ttgtacttta
     1621 tccgtaactc tgagtttgaa gtggactttt cccagtatat taccgacgcg gacaatgcca
     1681 tggcccagct cagcatctcg ctggaaagaa ttggaggctg gatacctctg atcttcttcg
     1741 agtacagccc tgtaaacgct gccaatattc ctggccagtt cagcgtcatc tttacttcac
     1801 agcagcaatc cgaagttacc aacggcttcc ggatcaccgt gaacgacaac gccggcagag
     1861 ctgtggccag tgcggacttc cagatgcacc tgctggagca ctttaacgct tctgtttcca
     1921 ccgctgagct gtatcaatac acgggccaga cagtgcagtt cagagacgtc acgctcggga
     1981 atcccaatta ctggcactgg gattttgagg atgacggcat catcgacagc aatgatcccc
     2041 atcccaccca tgtttataac agcgcgggag tgtacaacgt tcgcctcacc cttggtcaca
     2101 gcggtgccca ggaagaagat ttcatctttt tgcccggcta tatcaccatg tatggcacct
     2161 ccgtcacaga tgacgatatt ccccccacct ggaccctgca gggctctccc tataacctct
     2221 ttggcgacat cgtgatcgga gcgatggatg agatcgatat cgagccgaat gtggaagtga
     2281 atctctttgg caatgacccg ctgctggtaa acggggtgct gaatgccaac caggccaggt
     2341 tcaaacccca aaaccagaat ggccgctggg gtggcctgaa gttccagggc tccaatcaac
     2401 gcgaacccag cgagctcatc ggctgcgaga tcgtggacgc cctcctgccc atagacatag
     2461 aagacgccag ccccttgatt tcaggagtca ccatcacggt gagcgacacc actcagtatg
     2521 aggattcgcg gggtatccgc attcaggggg attcaagccc cagccttatc gatgtggagc
     2581 tgatcaacta ccggcagggt gtatcgatcc aaaacgatgg caacccagat cgcgacactc
     2641 ccaccctcac caacatcaga gtgagaaact ccagccagac cagccgggat gacctggacc
     2701 ccggcacggg gatattcatc tcaggcgcgg ctgtcctcaa caccgtgctg gtggataact
     2761 tcggttccgc gatcgttatc gacggcaccg aaaacaacac cactcccacc cttaccaata
     2821 tcagggtgcg aaactccagc cagaccagct ggggcgaccg ctatggcatc cagatcatgg
     2881 gaggggcggt ccccagcctg gaagatatcg atatagaaga tgttatctat ggcatccagg
     2941 tactgggcgg aggagatgtc cagcgagaca ctcccaccct caccaacatc agagtgagaa
     3001 actccagcca gaccagccgt tctgacacct ggggcatcag cattcaggac cttagcagca
     3061 tcgagctgga ggatgcccag tttgacggct ttgatacagg tatcaggatc aatgctcaga
     3121 cccgggtgga atccaccccc actctcacca acatcagagt gcgcaattcc agccagacca
     3181 gtcgccagga aaatatcggc atcgacatct ccggcagcgt gacggctcag atggacgacg
     3241 tgatcgtgga agattacccc tttggcatca tatacagtgg cagtctttcc agccgcgatg
     3301 tcagcactcc cacgctcacc aacatcagag tgcgcaactc cagccagacc agccgcaacc
     3361 tggccattgg cgtacaacta agcgatttgg ccagggtaca ctgcagtgat tttatcatcg
     3421 aagactacca gatcggcctt gagattagca acgacacatc actgcgggat gtcagcactc
     3481 ccacgctcac caatatcaga gtgcgcaaca gctcccagac cagccgttat gaaaatgccg
     3541 gcatcttcct cggagccgga gtgggaggta ctctcacagg ggctgaggtg gaagaagcca
     3601 ttatcggcat cttcattgcc gacggcaata ctaccaaagt ggaacccgcc aggatattca
     3661 actgcgagtc cgggatcaaa gctgccagtt ccttgctgcc caagtctctc cagcgccatc
     3721 tgatcgtgct ggagcctgcc tttctgaatg agcattttga ctggcagttc aaggccttcg
     3781 atctggaaat gcccggcccc tggctgattg aaaacaatac catcaatggc ctcaccacct
     3841 atgtgaaagc tgaagatgcc ggcatcctgt tcatcaacaa catcgcctgg accacttctc
     3901 cttctatgca gcccttccaa ctgcaaaact ctgtgttgaa tgcctcttat aacgatatgt
     3961 ttgtctcgcc tccctatccg ggaattggca atctcgcgct ctaccctgag tatgtcaacc
     4021 cggccgaacg ggaatttggc attacctacg acagcccctg catcgataca ggctctccgc
     4081 tgtccccaaa ggatgaggat ggcagcaggg ccgacatggg tgccttcccc tatctgcaca
     4141 gagcttccat ggtggtcagc cagcggtttg tccagcccaa tactcaggtg ttcttcacca
     4201 acaccagcat aggtcatgat cacctcaaca gcttctgcga atgggacctg aacaaggacg
     4261 gccaggctga agctacctcc agagactggg ctcacacctt cactgagccc ggagtctata
     4321 acctccggtt gaggatgcac tccggcaact tggaagatat tcgtgattat aacgctgtgg
     4381 tggtagtcca gagcgcgctg ctgcagggtc ctccccaggt gacgatcggc agactggcta
     4441 acgacatcgt tctggattgg gagcccgtta ccctcaccca gcaaggggat cccgtcgttg
     4501 tagaatacta cctgatctac agctgtgagg agcccgacgg gtattttgat ttcgtgggca
     4561 atacccaaaa ctttgagacc atctataccc acctgggtgg agctgaagcg gataagcgct
     4621 tttacttcgt gctgggcttc acggggacca tgagagagct gcagcagtat atcgaactgc
     4681 atcgccgcct ccgtcccagc tccatgcaga ctcctctgat gaggaacaag ccatagtcaa
     4741 agctaaggtt gggacccggc gtcccaaaag caggggtgga atttccacag ctctggtccc
     4801 tgccagtttg ctgggagggg tcagggcaag gagttccttg cctcagcagc gggatttcta
     4861 tgccgccatc ctctttctgg acgtgtcagg tttcacccgc atcaccgaag cagccagtgc
     4921 cagaggccat tatggcgtgg aactggtcac caaggtcttg aaccgttatt tcggcagggt
     4981 ccacaagctg atcactcctc tgggcggaga gatcgtcaag tttggcgggg atgcctgtct
     5041 gatcgtcatt cccatccagc ccggggcttg ttctgaagag cataagccag acatcccggc
     5101 cctgatagcg gccatccttg aggaagtttc caggctggac agacgtttca gaaaggagta
     5161 tggcttcccc ttcagcatcc acggtgcctg gggcaagggg atcgtgagtc tgagcgttgt
     5221 gggtgattcc agcaggcatc tggactacta cgtcagctct cccgacctca aagcagtcta
     5281 tgatttggcg gaaaccagcc aggcgggtca gatccaggga ccagccaccc acgcccagcc
     5341 gcctcctgag cttccagacc ttcctgccaa aggatccagg gcggccagat tgtttctgcc
     5401 ctcagacatc atccgcaagc tggaactaga gccccgtccg gcagagctcc gcaacgccac
     5461 ggtgctgttt atcaagcttt ctccctccag ctcagatgat attactctgg accaatatca
     5521 ggccgccttt catcgtatcc agtctctgat caccaaccat ctgggcctga tcaacaagat
     5581 cgacttcaat gagaaaggct atctcatcct ggccctcttt ggtgtcccct ttgtatatgg
     5641 caacgacaca ctgcgggctt tcagctctgc ttacaggata tcactcctgc ggctcgaggg
     5701 tatcagcatc cagatcggta tcacctacag caatatctat tgcgggatca tcggagctcc
     5761 ggctaggcat gaatatggga tcatcggcaa cgccgtcaac atcgcggccc gtctgatgag
     5821 ctttgcccag cccggagagg tctgcctttc caaggaactg atcccccatc tggaaggtca
     5881 gtttgagact gcctggctgg ccagggcttc cgtgaaaggc atcgcggaac ccttggatat
     5941 ctaccggctc ttgagaatgt tgccagcccg ctggggcagt ctggaagagc aatatcgcgc
     6001 tgatcccctt tttctggacc ccttgaagct acagactctg cagcaaggtc tggcgtcaca
     6061 gggtgaattt ctctgtctga tcagaggtga atccggcacc ggaaagagcc agctcatctt
     6121 caaactctgc gagccctggc tgaatagcga gcctcccttc caactcattc ctgccgaacc
     6181 tcatatgcgg gacctgcgtc tggaaatctt cttctggagc gcccgcaagg aactgggcat
     6241 caccaatttc cgggaagaat tgggccatat actgcaatgg tacgggtctc agactgccgg
     6301 ccccgaaccc gacctcactg aggacctacc cgatgtgcat atcctcagcg acctgctgtt
     6361 tggagctccg agcagctcca gccgccaaca aatcgccctg gacctgatcc tggacatcct
     6421 catccgcatg tatcctcagg gcaggatgct ggtgctggaa aacttccaca atttcgatgc
     6481 ccagagccgt gatctgctcc tgcgcctgat ccgccacaag ctctatcagg gcgataagat
     6541 cattgtaagc agcgaggcca ctctccccga tgagctttgc cagggcttca gctttgccgg
     6601 ggtctgtctg gagaattggg acgaggagct cagcggccgt tatataagac accgggtgcc
     6661 aaacatcacc acccgggcca ttcaactgct gcatcagatc tcagaaggaa atccccggtt
     6721 tctgagaggt ctcaccggcc atatccagac gcattggtct gcctcccagg acctgattac
     6781 tcatcagatc atagagtcga tgcgggcccg gggtttgctg cctaacgatc tggaaaacct
     6841 cttcagggca gagtatgagg ccctgaacac ttccgaacag gtctttacgc gtctggccag
     6901 catctatggc agaccgttcc ggctgagtga tttcatgcag attttcccaa cccacagctt
     6961 ctccagcctg gctggcagcg cggaccggct actccagctt ggagtcctga ggcatgaagc
     7021 cggctccgat gcccaggtgc tctggtttgt aaacccgctt ctcccggaga cggtctatcg
     7081 cagcatcctg cagagtgaaa agctggcctt ccaccgctcc atcgccacct actattccag
     7141 cctgcaaagc caggatgaac tggtctggga cctgatcgcc catcactggc tgagggctga
     7201 agacaggccc aagatagcct ggtggtgcaa gaagctggca gaccattact atatctccgg
     7261 agcttttgaa ctgagcctgc ggatgtggca gcagctcgtc ctttggcccc tgaatgaaga
     7321 cgcccacacc caggcccagc tgaaatgcgc cgaactctat ctcatactgg cagataacga
     7381 taaagccggg cgaatcctgg aaagctatca gcacctggcc caccaacccg caaaacactg
     7441 cgaaagctgg atatatctga gcgccaggct gatgatcaat cgctcccaat actctgaact
     7501 ggatgatttc ctggcccagc acgccgaccg catccaggac cccgtcatca aagacaagct
     7561 cgacacctcc cactgcgaag ctatcctgca aagcatggac cccgcgcgca tcgaagccaa
     7621 agccctgccg ctctggaaca ggctcaaaga gcaggagcgt catctgtccc tgaattcctt
     7681 ggccggcatc ataggcagtt attacatcaa ccggggaaac tatcagaccg ctgcctccta
     7741 ttacaaagag aagatcaagc tggccaaacg cctcaaagac cccgtctcga tgcggatcgg
     7801 cttatccgga ctgggcattg ccttatcccg gatggggcag aaaaccaagg ccctggaaca
     7861 ttatcagcag gctctcgccc tggcttccaa aagcggcgat cgcaacggct ttagcaaagc
     7921 cctgctggac ctgggagtct atcaccgcaa cgagggagac tatcccaaag ccctgcaata
     7981 ctaccagcag agcctcagcc tggcagaata catcggcaac aagctccaaa tctcgatcgt
     8041 gatctacgac ctcggcgagc ttcactacta tcagcaaaac tgggacgaag ccgaaagcta
     8101 tatcagcaaa tccctggaac tggcaaaaca actgggagac caggtaggca tgagcttctg
     8161 ctacgacgct ctgggagaca tcaacttcca gcgcaaaaaa ttccctgaag ccctggccat
     8221 ctaccgggcc aacctattgg tgcagcaccg cctcaacgac atggaaggca aggcccacac
     8281 cctgggcaat ctgggaaacc tggccaaaag cgagaaaaac tatcagcggg ccaccaagct
     8341 atatggatcc cagataacca tcctccagaa agtgggagat attgacggag agggccgtgc
     8401 ctggtttaac ctcgccatgc tggattttga acagcaaaac aaagaggccg cccgtcccaa
     8461 gctgacaaaa gccctggaac tctttgaaag ctgtaacgca cgctactata tagacatcac
     8521 gcg
//
LOCUS       DAOSFN010000113         8487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_7141_length_8487_cov_9.622391, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000113 DAOSFN010000000
VERSION     DAOSFN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7141_length_8487_cov_9.622391"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..783)
                     /locus_tag="PLO35_07450"
     CDS             complement(76..783)
                     /locus_tag="PLO35_07450"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOH79542.1"
                     /translation="MPERPVIGLSMNYTLLGSYEQFHIRNRYLDAIFDHGGLPLPLPC
                     TPDRDLVRQYLAKIDALVIIGGLDYPSQMYNQAPHPRLDAMHERRAGSDIPLFEEALQ
                     TGMPILGICAGAQLINIALGGSLIQHLEPGEMHIKENYHPVQVLGGHWLPAIFGSDAL
                     TVNSNHHQALDPQRIGLGLRVVALAEDGVVEAVELDVPQMVLGLQWHPERITDLSHRA
                     MVFEFLAKEALTYRNLK"
     gene            complement(776..1474)
                     /locus_tag="PLO35_07455"
     CDS             complement(776..1474)
                     /locus_tag="PLO35_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79543.1"
                     /translation="MPTLTDKFILSDPSQLKRIKRHQYLMQAVPVLLILISFAIGSRP
                     LLYIPAIVLAGLGAGLFILIGAAHRFHQRIPIPEAGMFVSPIQGKLRYSRGNNEISVV
                     NIGRVFLDSVELRSPHPAAEIQDGQLSVPTPYGTITLRFNKAKVTWFKDPDFTRGNVI
                     GMIWGHGSCTISIPARVLGADQESSPDELIIPKVGKALDICDPLFQLSDPPKAEPAGQ
                     QEHSDLTESAEDHA"
     assembly_gap    1751..1850
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2033..2470)
                     /locus_tag="PLO35_07460"
     CDS             complement(2033..2470)
                     /locus_tag="PLO35_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOH79544.1"
                     /translation="MKHLLIAIILIALCLPLLALKEDDILGSWYNGEKSSKIQIYKTT
                     GGSYAGKIVWLKEPNDAKGVAKLDPKNPDKKLRGRALMNLVILTGLKSKGNDKFDGGK
                     IYDPKSGNTYSCKGELENINTLKLRGFVGVSLIGRTDTWTRAK"
     gene            complement(2495..3787)
                     /gene="purB"
                     /locus_tag="PLO35_07465"
     CDS             complement(2495..3787)
                     /gene="purB"
                     /locus_tag="PLO35_07465"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOH79545.1"
                     /translation="MIPRYSLPEMERVWTSENRYECWLEVELAAARAMFELGIIPETD
                     YQSIAEKADFNADRIEEIEAVTKHDVIAFLTNVAEYVGPSARWIHYGMTSSDVLDTAT
                     ALQLKQAGELILTQAHRLAETLRLQARAHRHTVCMGRSHGIHAEPTSFGLKFALWFDE
                     CKRDIARLQDAIDGISVGQFSGAVGNYAHLDPSVEELACRYLDLKPVNISTQVIQRDR
                     HAHFLHVIALLGSLLEKIALEVRHLQRTEVQEAEENFTKGQKGSSAMPHKRNPIVSEQ
                     LCGLARLLRSNAHAAMENNALWHERDISHSSVERVILPDSCILIHYMLERAITLIGNL
                     LLYPDTMKANLEITGGLVFSQALLLRLTNAGLSREEAYALVQENAMVCWESGKPLMDK
                     VLADKRITSVIPPEEIRDIFDYALYLRHVDLIFQRCGI"
     gene            complement(3784..4509)
                     /locus_tag="PLO35_07470"
     CDS             complement(3784..4509)
                     /locus_tag="PLO35_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79546.1"
                     /translation="MNRLVPLIFILITLMLSSCAKDKLKPLSIEARKELLRAEMLKWE
                     NLRGEGVTNLNYMGLTLRRMFVFSKTKDETRFDVIDGGVLGAGASPLISVYLGEYFSL
                     SSDYFPQLELMARTLVDPRMSMAPIKDINALVETYADSILATGRLVHEGIEISFDPQF
                     RLQRVFDPRSKSEALFSYNAKGLPDKLSFTMSNASAELLFDSVEYGTATIEALPRQEG
                     SILDQFLEPDTFDEIAPLEAEEP"
     gene            complement(4506..5327)
                     /gene="lgt"
                     /locus_tag="PLO35_07475"
     CDS             complement(4506..5327)
                     /gene="lgt"
                     /locus_tag="PLO35_07475"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOH79547.1"
                     /translation="MIRHNLNPDIVSFDLAGLHLQVRWYGLFYVLSFILAWLFYRKLL
                     KFRGIHLSREQYESVIFVMMLGVVLGGRIGYALFYNLPYYFQHPLQLLAVWEGGMSFH
                     GGALGVIIAGYLFVRKNKLSFAAMADAVIPLVSIGLGLGRLGNFINGELWGRPTTLPW
                     GMVFPEAGDLPRHPTQLYELFLEGIVLFAVTFWLLRKIKTPGVVFWIWFALYGIFRFL
                     IEFVREPDDIQIYQDYGYFFGFMSIGQFLSMLMIIVAAIAIWLIYRQPPKTEVHK"
     gene            complement(5338..6120)
                     /locus_tag="PLO35_07480"
     CDS             complement(5338..6120)
                     /locus_tag="PLO35_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOH79548.1"
                     /translation="MKLIVCVKQVPNTTEIKIDPVTNTLVREGVESILNPFDAYAIEE
                     AVRLKEQHGGTLTVISMGPAQVETTLREAISLGADEIILLSDRKFAGADTWATSLTLA
                     AAIRKLGSYDLILTGQQAIDGDTAQVGPGIAAHLKIPQTCFVRKIEEVKPCHLIVQRL
                     MEDGYDRVQMKLPGLISVVKEINTPRLPSLRGKRNAKSAELKVWNAADLELDEKDIGL
                     NGSPTQVVKIFTPKHDKLTEKFHGDPDEAVEKIVTQLHKLTR"
     gene            complement(6133..7236)
                     /locus_tag="PLO35_07485"
     CDS             complement(6133..7236)
                     /locus_tag="PLO35_07485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOH79549.1"
                     /translation="MKVPMLDLHAQYDPLLNDIKAAIDKVLADHHYIMGPQIKELEEK
                     IATDLGIAHAIGCASGTDALVLAIKALGIGEGDEIITTPFTFFATASSIWRNHARPRF
                     VDIDPRTFNLDPSLIEAAITPKTKAIMPVHLFGQSADMTAIMEIAKKHGLAVIEDNAQ
                     GIGCTWDGKMSCSYGDIGTLSFFPSKNLGAMGDAGMCLTNSDDLAAKLRQLRVHGENP
                     KYYHKWVGLNSRMDTLQAAILNVKYDHLAAWSEARRANAAFYNEHLSDIVQIRTPWID
                     PRAVSIYNQYTLICDDRDGLMKHLQDNGIGCAVYYPLPLHMQECFSELGYKAGDLPVS
                     ETMARKVLSIPIYPELTNEQKEYVCDSIRAFYN"
     gene            complement(7251..>8487)
                     /locus_tag="PLO35_07490"
     CDS             complement(7251..>8487)
                     /locus_tag="PLO35_07490"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOH79550.1"
                     /translation="VPHPGTYQLTVFSRLSDAVKASRGELPQISDPLTMSPLQQKAVE
                     EDSLYANFQGLRQLRLTRQGKSQVYDLLRFMRLGELEQNPLLKDGDLIWVPTLEQSVS
                     VMGSVYLPGEYQFVPGDKLGDLLRLCQGFTPEANLKRILIYRYQPNGVDFDILSQDIS
                     AVASLSDMGFALMPRDRIIVGQDSEARRGWKVKVEGAVKTPGEFLIGDKSTLWDILQL
                     CGGVNSRGDLGNAVLINGPYSAKLFPDLERLKAFSMGQMSPVEYSYLRSSLRQLRGKY
                     SISLQECWDSSGQSSNPVLQDGDYIFVPEQFDMIAVSGQVRNPGLVPWVEGKDWKYYV
                     EACGGYTNNRKVGGVRIIRDHSGNWVKPSKKLALRPGDMVFVAESTDRDLWADIKDVA
                     LLASQIVTIFLSIRAITSN"
BASE COUNT         1692 a         2258 c         2540 g         1897 t
ORIGIN      
        1 cgggactcag aatgccattt tcgcacaagc tctgaagcca gtgacaccaa ttattcaacg
       61 gtcttttgat ggttattact tgaggtttct gtaagttagc gcttctttag caagaaattc
      121 aaagaccata gctctgtgcg ataggtccgt gatccgctcc ggatgccact gcaatcccag
      181 aaccatctgg ggcacatcca gttccaccgc ttccaccact ccgtcctcag cgagggccac
      241 cactctgagt cccaagccga tccgctgggg atcgagggcc tgatgatggt tggaattcac
      301 cgtcagagcg tcgcttccaa agatggcagg tagccagtgc cctcccaata cctggacggg
      361 atggtagttt tctttgatgt gcatctctcc cggctccaga tgctggatca gtgagcctcc
      421 cagggcaatg ttgatcaatt gcgcgccggc acagataccc aggatgggca tgccggtctg
      481 caaagcttct tcaaagagcg ggatatcaga gccggcgcgg cgttcatgca tcgcgtccag
      541 cctgggatgg ggcgcctgat tatacatctg ggaaggatag tcgaggccgc cgatgatgac
      601 cagggcgtcg atttttgcca gatactgccg gaccagatcg cgatccggag tgcagggcaa
      661 gggcaaaggt aagccgccgt ggtcgaagat ggcgtccaga tagcggttgc ggatgtggaa
      721 ctgctcgtag ctgcccagca gggtgtagtt catgctgagg ccaatgacag gccgttcagg
      781 catgatcttc cgcgctctca gttagatcag aatgctcttg ctgcccggca ggctccgctt
      841 tgggaggatc gctgagctgg aacagaggat cgcagatatc cagcgctttg cccactttgg
      901 ggatgatcag ctcatccgga gagctctcct gatcagctcc caaaaccctg gcgggaatgg
      961 agatcgtgca ggagccatgc ccccagatca ttccgatcac gttgccgcgg gtgaaatccg
     1021 gatctttaaa ccaggtgact ttggctttgt tgaagcgcaa agtgatggtc ccataagggg
     1081 tggggacgct tagctggcca tcctggattt cggctgcggg atggggtgaa cgcagttcca
     1141 cggaatccag gaagacgcga ccgatgttga ccacgctgat ctcgttattg ccgcggctgt
     1201 aacgcagctt tccctggatg ggggagacaa acatcccagc ctcggggatg gggatacgct
     1261 ggtggaaacg gtgcgccgcg ccgatcagga taaagagtcc cgcccccagt ccggccagca
     1321 cgattgccgg aatatagagc aagggcctgg acccgatcgc gaacgagatc aggatcagca
     1381 acaccggcac ggcctgcatc agatactgat gacgcttgat gcgcttgagc tgagacggat
     1441 ctgagaggat gaatttgtct gtgagggtag gcatagtcta ttccaatatt gtgatgttag
     1501 ctaaataatc agggctcgtc ttggcgttca aagtgttgtc cttcggtgca ctggttgaag
     1561 tccaagcggc ccgcaaactc gctgccatat atacctttat cgtggccgct tgaactccaa
     1621 cggggctcgg cttggagttc aaagtgttgt ccttcggtgc actggttgaa gtccaagcag
     1681 tactttggcg cgcgtgtgtt gtccttcggt gcactggttg aagtccaagc ggcccgcaaa
     1741 ctcgctgcca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaactcgctg
     1861 ccatatatac ctttatcgtg gccgcttgaa ctccaacggg gctcgtcttg gcgttcaaag
     1921 tgttgtcctt cggtgcactg gttgaagtcc aagcggcccg caaactcgct gccatatata
     1981 cctttatcgt ggccgcttga actccaacgg gataaatgcg ggcgggggtg gcttacttgg
     2041 cgcgggtcca ggtgtcggtg cggccgatca gggaaactcc gacaaagccg cgtagcttga
     2101 gggtgttgat gttttccagt tcgcctttgc agctgtaggt gttgccggac ttgggatcgt
     2161 agatcttgcc gccgtcgaac ttgtcattgc ccttggactt gaggccggtg aggatcacca
     2221 ggttcatgag ggcgcgtccc cgcagcttct tatccgggtt tttggggtcg agcttggcta
     2281 cacctttggc gtcgttgggt tctttgagcc agacgatctt gccggcatag ctgccgccgg
     2341 tggttttgta gatctggatt ttgctggatt tttcgccgtt gtaccagctg ccgaggatgt
     2401 cgtcctcttt gagggccagc aagggcaggc acagggcgat caggatgatg gctataagca
     2461 ggtgtttcat tgacttactc ctttcgcggg agcttcatat cccgcagcgt tggaagatga
     2521 ggtccacgtg gcgcagatag agtgcgtaat caaagatgtc gcggatctcc tcagggggga
     2581 tcacgctggt gattcttttg tctgccagca ctttatccat cagaggcttg ccgctttccc
     2641 agcagaccat ggcgttttcc tgcaccaggg cataggcttc ctcgcgactg agaccggcat
     2701 tggtgagccg gagcagcagg gcctgagaaa agaccaggcc tccggtgatt tcgaggttgg
     2761 ctttcatagt gtccggatac agcagcagat tgccgatcag ggtgatggcg cgttccagca
     2821 tgtagtggat gaggatgcag ctgtccggca ggatgacgcg ctccacgctg gagtggctaa
     2881 tatcgcgttc gtgccagagg gcgttgtttt ccatggcggc atgggcattg gaacgcagca
     2941 gtctggccag gccgcagagt tgttcgctca cgatgggatt gcgtttgtgg ggcatggcgc
     3001 tgctgccctt ttggcctttg gtaaagtttt cttccgcttc ctggacttcg gtgcgctgga
     3061 ggtgacggac ctcgagggcg atcttttcca gcagcgagcc taaaagagcg atcacgtgga
     3121 ggaagtgggc atggcggtcg cgttggatca cttgggtgga aatattcacc ggcttgaggt
     3181 ccaggtagcg gcaggccagc tcttccaccg aggggtccag atgagcgtaa ttgcctacgg
     3241 cgccagagaa ttggcccacg ctgatgccgt cgatggcatc ctgcagccgg gcaatgtcac
     3301 gcttgcactc atcaaaccag agggcaaact tgaggccaaa actggtgggc tcggcatgga
     3361 tgccgtggga gcgtcccata cagacggtgt ggcgatgggc ccgggcttgc aatctgaggg
     3421 tttcggcgag gcgatgggcc tgagtgagga tcagttctcc cgcctgtttc aactgcaggg
     3481 ccgtggccgt gtccagcacg tccgaagagg tcatgccata atgaatccag cgggctgagg
     3541 gacccacgta ttccgccacg ttggtgagga aggcgatgac gtcgtgcttg gtgacggctt
     3601 cgatctcctc gatgcggtcg gcgttgaaat ccgccttttc ggcaatgctc tgatagtcag
     3661 tctcgggaat gatgcccagt tcaaacatcg ccctggcggc ggccagttct acttccagcc
     3721 agcattcata gcggttttcg ctggtccaga cgcgctccat ttccggcagg ctgtagcgcg
     3781 ggatcatggt tcctctgcct ccagaggcgc gatttcgtca aaggtatcgg gctccagaaa
     3841 ctggtccaga atgctgcctt cctggcgtgg cagggcttcg atggtggccg ttccatactc
     3901 cacgctgtca aaaagcagct ccgcgctggc gttgctcatc gtgaagctca gcttgtccgg
     3961 caggcctttg gcgttgtagc tgaagagggc ttcgcttttg ctgcggggat caaagacgcg
     4021 ctgcaagcgg aactggggat cgaagctgat ctcgatgccc tcatggacca gccttccggt
     4081 ggccaggatg gaatccgcgt aggtctctac cagcgcgttg atatccttga tgggggccat
     4141 agacatcctg ggatcgacaa gtgtccgggc catcagttcc aactggggaa aataatccga
     4201 gctcaggctg aaatactctc ccagatacac agagatcagg ggtgaggccc cggcacccag
     4261 cacccctccg tcgatcacgt caaagcgcgt ctcgtctttg gtcttggaaa agacaaacat
     4321 ccggcgcagg gtgaggccca tgtagttgag gttcgtcact ccttctccgc gcaggttttc
     4381 ccacttgagc atttcggccc ggaggagttc tttgcgggct tcgatggaga ggggtttgag
     4441 cttgtctttg gcgcaggaac tcagcatcag agtgatgagg ataaagatga gggggaccag
     4501 gcggttcatt tgtggacctc cgttttgggc ggttgacgat agatcagcca gatggcaatg
     4561 gcagccacga tgatcatcag catgctcaga aactgaccga tagacataaa gccaaagaaa
     4621 tagccgtagt cctgatagat ttgaatgtcg tccggctctc tcacaaactc gatcaggaag
     4681 cggaagatgc catagagggc aaaccaaatc cagaagacca cgccgggagt cttgatcttc
     4741 ctgagcagcc agaaggtgac cgcgaagagg acaatcccct ccagaaacag ttcatagagc
     4801 tgggtgggat gcctgggcag gtctcccgcc tcagggaaaa ccattcccca gggcagcgtg
     4861 gtgggccttc cccatagctc tccgttaata aagttaccca gcctgcccag ccccaaaccg
     4921 atcgagacca gcgggatcac ggcatcggcc atggcggcaa agctgagctt gttttttctc
     4981 acaaacaggt agccggcgat gattacaccc aaagccccgc cgtgaaagct catgccgcct
     5041 tcccagacgg ccaaaagctg caggggatgc tggaaataat agggcagatt gtagaagagg
     5101 gcatagccga tgcggcctcc cagcaccact cccagcatca tcacaaagat gacggattca
     5161 tactgctcgc ggctgaggtg gatgcctcgg aacttgagca gtttgcggta aaacagccag
     5221 gccaggatga agctcagcac ataaaagagc ccataccagc ggacctgcag atgcaagcca
     5281 gccagatcaa agctcacgat atcggggttg aggttgtggc gtatcactgt tcctgcctca
     5341 gcgggtgagc ttgtggagct gggtcacgat cttttccacc gcttcgtccg ggtcaccgtg
     5401 aaacttctcg gtgagcttgt cgtgtttggg agtgaagatt ttaaccacct gggtggggga
     5461 accgttgagg ccgatgtcct tttcgtccag ttccaggtcc gcggcgttcc acactttgag
     5521 ctcggcgctt ttggcgttgc gcttgccccg cagggatggc agccggggag tgttgatctc
     5581 cttgaccacc gagatcaggc ctggcagctt catctggacc cggtcatagc cgtcttccat
     5641 gaggcgctgg acgatcagat ggcagggctt gacctcctcg atcttgcgca cgaaacaggt
     5701 ctggggaatc ttgaggtgag cggcaatgcc aggccccacc tgggctgtat cgccatcgat
     5761 ggcctgctgg cctgtgagga tgaggtcata agacccaagc ttgcggatgg cggccgccaa
     5821 agtgagagag gtggcccagg tatcggcccc ggcaaacttg cggtccgaca gcaggatgat
     5881 ctcgtcagcc cccagcgaga tggcttcccg cagggttgtt tccacctgag cggggcccat
     5941 gctgatcacg gtcagggtgc cgccatgctg ttccttgagg cggacggcct cttcgatggc
     6001 ataggcgtcg aaggggttga ggatgctctc cacgccttcc cggaccaggg tgttggtcac
     6061 cggatcgatc ttgatctcgg tagtgttggg cacctgttta acgcagacga tcaatttcat
     6121 agcgggctac tcctaattgt agaaagcgcg gatgctgtcg cagacatact ccttctgctc
     6181 attggtaagc tctggataga tagggatgga gagcaccttg cgggccatag tctcgctcac
     6241 cggaaggtct ccggctttgt atcccaactc gctgaagcac tcctgcatat gcagcggcag
     6301 cggataatag acggcgcagc ctatgccatt gtcctgcagg tgcttcatca ggccatcccg
     6361 gtcgtcgcag atgagggtat attggttgta gatgctcacg gcgcggggat cgatccaggg
     6421 agtgcggatc tggacgatat cagagaggtg ttcgttgtag aaagcggcat tggcgcgtct
     6481 ggcttcgctc caagcggcca gatggtcgta tttgacgttg aggatggcgg cctgaagggt
     6541 atccatgcgg ctgttgagac ccacccattt gtgataatac ttggggtttt cgccatgcac
     6601 tctgagctgc cgaagcttgg cagccaggtc gtcagaattg gtgaggcaca tacccgcgtc
     6661 acccatggcg ccgaggtttt tgctggggaa gaagctgaga gtgccgatgt cgccatagga
     6721 acagctcatc ttgccgtccc aggtgcagcc gatgccctga gcgttatctt cgatcacggc
     6781 caggccgtgt tttttggcga tctccatgat ggcggtcata tcggcgctct ggccaaagag
     6841 atgcaccggc atgatggcct tggttttagg tgtgatggcc gcttcgatca gagagggatc
     6901 gaggttgaaa gtgcggggat cgatatccac aaaacggggc cgggcatggt tgcgccagat
     6961 cgaggaagcc gtggcaaaga aggtgaaggg cgtggtgatg atctcatccc cttcgccgat
     7021 gcccaaagcc ttgatggcca acaccaaagc gtccgttccg gaagcgcagc caatggcgtg
     7081 ggcgatgccc aggtccgtgg cgatcttttc ttccagttct ttgatctggg ggcccatgat
     7141 gtagtggtga tcggccagca ctttgtcgat ggcggccttg atgtcgttca acaatggatc
     7201 atactgtgca tgcaggtcca gcatagggac tttcattcaa aactcctgta ttaatttgat
     7261 gttattgctc tgatacttaa gaagatggtt acgatttgcg aggccagcag ggccacgtct
     7321 ttgatgtcgg cccagaggtc gcggtcggtg ctttcagcta cgaagaccat gtctccgggc
     7381 cgcaaagcca gctttttgct gggtttgacc cagttgccgc tgtggtcgcg gataatcctc
     7441 acgcccccca ctttacggtt gttggtatag cctccgcagg cttcgacgta gtatttccag
     7501 tccttgcctt ccacccaggg caccagaccg ggattgcgga cttgtccgct caccgcgatc
     7561 atgtcaaact gctccggcac aaagatataa tctccatctt gcagaaccgg gttggagctc
     7621 tggccgctcg aatcccagca ctcctgaagg ctgatgctgt atttgcccct gagctgacgc
     7681 aggctgctgc gcaaatagct gtattccacc gggctcatct gccccatgct gaatgctttg
     7741 aggcgctcca ggtccgggaa gagcttggca ctgtagggtc cattgatgag gacggcgtta
     7801 cccaggtcac ctctcgaatt gacgccacca cagagctgca ggatgtccca cagcgtgctc
     7861 ttgtctccga tgagaaactc gccgggagtc ttgacagccc cttccacctt cactttccag
     7921 cctcgtctgg cctcgctgtc ctggcccaca atgatccggt cgcgcggcat caacgcgaag
     7981 cccatatcgc tcagactggc cacagcgctg atatcctggc tcaggatgtc aaaatccact
     8041 ccgttgggct gataacgata gatgaggatg cgtttgagat tggcctcggg agtgaagccc
     8101 tggcagagcc tgagcaggtc gcccagcttg tcgccaggca caaactgata ttcaccaggc
     8161 agatagacgc tgcccatcac gctcacgctc tgctccagag tgggcaccca gatcaggtcg
     8221 ccgtccttga gcagcggatt ttgctccagc tcgcccaggc gcataaacct tagcaggtca
     8281 tagacctggc tcttgccctg gcgggtgagg cgcagctgcc tgagcccctg gaaattcgca
     8341 tagagcgaat cttcctccac cgctttttgc tgcagggggc tcatcgtgag agggtctgat
     8401 atctgaggaa gctctccccg gctggccttg acggcatccg aaagcctgga aaacaccgtg
     8461 agctgatagg tgccgggatg gggcaca
//
LOCUS       DAOSFN010000114         8444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_7177_length_8444_cov_8.466444, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000114 DAOSFN010000000
VERSION     DAOSFN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7177_length_8444_cov_8.466444"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3018
                     /locus_tag="PLO35_07495"
     CDS             <1..3018
                     /locus_tag="PLO35_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOH79551.1"
                     /translation="VDWKVEYSVNGGSSWIQTGSSFTASSSDTPQTFSSTLNLNGDIR
                     IRIKRATGTGTSNARLNIDDITLTDYVATLTLAPSSLTGFTYVQDEGPSDPQTYTISG
                     QNLSPASGNISIAGAAAFEFSSTGSSYSSSLLVPYNDSQLSATVYVRMISGLSAGSYN
                     QDASHTGGGAEAQLLGLSGTVNEPAVYATDLFISEYIEGSSNNKAIEIFNGTGAPVDL
                     SNYKLELYSNGSATPSSTLILSGSLATSDAFVIANSLSNSSILAVADLTNNGVINYNG
                     NDAIALKKISTGAFVDIFGVIGNDPGTAWTADGGYSTQDKTLVRKPTVISGVTQNPVG
                     TGATAFTTLDTEWDLYPIDTISDLGTHSFGGGGQSVSAPTIQASNIVAYPSNDEIALE
                     WTAGNGSRRIVKINASNSFTAPADGTQPTANPVYSGSGEQVIFDGATQIIEGTPFNGC
                     LVSGLQPNTTYWFRIYEYNGTGITTRFLTTSAPDNPSPATTSNTTGIGYYAGISGYGS
                     SIKSSLHALLRTTHTTQYSYDALWTQLPYTDEDPANTNNVIELYTGWSVPKNHYGGGT
                     TEWNREHTWSKSHGDFGETRPAGTDLHHMRPCDATVNSAKSNRDFDDGGTAYTDASPY
                     PGYSGVTGCYTSSNTWEPRNEDKGDVARMIMYMAVRYEGTDTSYDLEIVDYTNTAPDN
                     EPLYGKLSTLLNWHVQDPPDARERQRNDRIFERQGNRNPFIDYPSYAQYIWSPVPTGP
                     TSLTQTGFTANWSTPISATKYYLQVATDSLFSSFVSGYSNYDAALSNAKTIAGLSNTN
                     TYYYRLRSYFLSGYSMYSPWVAVNLLPNGTALIAAQSSVYEYDLQGAQLQLNLQNASF
                     SDPSLNLANFTLLNGPAGLSIQSVQYLSSNSARLVLAFNGSDFDDNRQISILISPSEL
                     TQSTAITSSSLQLIAYVETLLSIDLQSGSLVLNIIPVSQASSYRIFGAADPYDDFINI
                     SAQGSFEVLVPTRWTTPSPTAPRAFYKAVGIRD"
     gene            3030..4217
                     /locus_tag="PLO35_07500"
     CDS             3030..4217
                     /locus_tag="PLO35_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOH79552.1"
                     /translation="MSPNRSKTQHLPFAIDGVEAFNQLLPEMMKNLLHNLPGIAYRCK
                     NDSKWTMLFMSEGCFEVTGYFPEEILYNSLMSYGDMIYEDDRALVRDAVSEGVTKKRQ
                     YRMEYRIIRKDGSLRWVWEQGSAVYRESGSPLYLDGFITDVTHRRELETMLKERADQL
                     SELNQMKDRFFSTIVHDLQNPVYSFISLSDFICQNYDHFKRAEIMEFLTQIRDAAQGM
                     NLLLNNLMDWTRVQTGKMKLQKDFFRLRAVIEDGLEGAHDQINKKNLNLVMSIPDDLQ
                     LETDRQVVASLFRNLLSNAIKYSQTRGKIIIAALTTEQDLQLSVQDEGVGIPRAELPD
                     LFKMDRDYRRPGTINESGTGLGLLLVKELMDLLAGRVEVQSRVNKGTKVTLFLPIELK
                     HLS"
     gene            complement(4248..4991)
                     /gene="phnC"
                     /locus_tag="PLO35_07505"
     CDS             complement(4248..4991)
                     /gene="phnC"
                     /locus_tag="PLO35_07505"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOH79553.1"
                     /translation="MVPQLEIKSLKKSYDGRYFAIDDVSFEVRQGDFIILLGLSGSGK
                     STLLRCVNRLIEPSSGDILFNGSSIRGLNGANLRHYRRRIGMVFQQFNLVKNLSVLTN
                     VLTGRLGYHGALSGFNSTDLETAYRNLERVGLKDMARRQVKQLSGGQQQRVGIARALM
                     QEPQIVLADEPVASLDPATADSIMQYLGEINREGITVICSLHFLTLARRYGNRVLALK
                     DGKVVFEGVPNQIDNERFKQIYGQDAEEI"
     gene            complement(4994..5923)
                     /locus_tag="PLO35_07510"
     CDS             complement(4994..5923)
                     /locus_tag="PLO35_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOH79554.1"
                     /translation="MRIKLITIIVLLFVLTSACMSESGRIDKANKALGSKRNPIKMYF
                     VPSLEAGKVVASGEAIAKALQAETGYYFKVAVPTSYAAVIEALGSYQADIAWLPTFAY
                     VLAKQKYDAEVKFMTVRNGLKQYRGQFVARADSPIKTIEDIQGKVVAYTDAASTSGYI
                     YPSAMLKEQGIEPSQTRFTGSHNAAILAVYSGTADVGCTYWSPPDSSGLPKDAREKLL
                     DTKPDVLEKIIPIAFTDWIPNDTVTFRKNMPQELEAKVVKALSEFAASAKGGDILRSL
                     YDIDGLQYATDADYDVVRKTLQLMKLDPSSFLN"
     gene            complement(6023..7294)
                     /locus_tag="PLO35_07515"
     CDS             complement(6023..7294)
                     /locus_tag="PLO35_07515"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOH79555.1"
                     /translation="MSIKIVSVDPAGLASRYDIQPGDRVLRINGHSINDQMDLEYYSS
                     DYQLDVSIEAADGKKRRFTILRDSFLPLGLETDPKPVKRCHNHCVFCFIDQMPPGLRS
                     SLYVKDDDYLYSRDYGNYITLTNLSEAEIKRVLTQHISPLYISVHTSSKDLRQRMMGY
                     KQEFDLLRVLKRLSRAGIAYHTQIVCVPGYNDGEELNRTVKDLLRPSLATLSIGVVPV
                     GLTRFRQGLPDLKPFDRLSATTLLKQLDKLRELDRGDILYAADEFYVLSGKRLPPPQY
                     YQDYPQAENGIGLLSLLRQGFSKRRKAFITELDKHPASYLLLHSRAATQEITRIVTRL
                     SASLDKSQLHNREIRNLFMGPHISVSGLLTAEDICRQHDSQPKQTLILPGSMFNDDGI
                     SLDGLSASELARQLARELLIVDPLFDGWHRV"
     gene            complement(7319..8443)
                     /locus_tag="PLO35_07520"
     CDS             complement(7319..8443)
                     /locus_tag="PLO35_07520"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH79556.1"
                     /translation="MKNTKTLLTAWLFIMSLSLAWADISVYFKNERKTVALPETKIAE
                     KLYVDFDAFNQVFNSIVKQERGDHRLHLFLNGKQFIFLIGSPFYSFENESWNLHLNVT
                     RSGNQFYLPSVFVTEHLPTHFKDMEMKGTVLQIERPKDRSVKVIVLDPGHGGNDPGAV
                     GKKLKLREKEVNLNVCLKLKQMLEKELGITVLMTRNDDRFITLKNRTKFANEHRADLF
                     VSVHTNASKNRAASGLETYYLSTALTSDARAVEALENDVVEEYEGGKAERAKYDALAF
                     ILSDLSQTEHLENSNNMALLIQQNLVAGTKSFDRGVKQANFYVLRGAFMPAVLIEIGF
                     ISNPDEEQKLANSQYQERLARTIFEGLKRFKFRYDRIRNT"
BASE COUNT         1981 a         2320 c         2202 g         1941 t
ORIGIN      
        1 gtggattgga aggtggaata cagcgtcaat ggcggcagct cctggattca aaccggcagc
       61 tcttttaccg ccagcagttc tgacaccccc caaaccttca gctccaccct caacctgaat
      121 ggcgatatca ggatcagaat caagcgggca actgggactg gtacctccaa cgcgcgccta
      181 aatatagacg atatcacttt gaccgactac gtggccactt tgacgcttgc tcccagcagc
      241 ctcacgggct tcacatatgt ccaggacgag gggccgtccg atccccaaac ttacacgatt
      301 tccggccaga acctctcccc cgcttccgga aatatatcca tcgcaggggc cgcggctttc
      361 gagttttcca gcacaggcag cagctacagc tccagtctgc tggtccctta taatgattcc
      421 cagttaagcg ccactgttta cgtgagaatg atctctggcc tgagcgctgg aagctacaat
      481 caagacgcca gccacacggg cggaggagcc gaagctcagc ttttaggctt atccggcact
      541 gtgaacgaac cggccgtcta tgccacagat ctctttatct cggagtatat tgaaggaagc
      601 agcaacaaca aggccattga gatattcaac gggacaggcg ctcctgtaga tctgagtaat
      661 tacaagctag agctttattc aaatggttct gccactcctt cttccacttt gatcctatca
      721 ggcagcctgg ctacttcaga tgctttcgtc attgccaatt cattatcaaa ctccagcatt
      781 ttggcagtcg cggacctgac aaacaacgga gtgatcaatt acaacggaaa cgatgccatc
      841 gccctcaaaa aaatcagcac cggagccttt gtggacatct ttggcgtgat tggaaacgat
      901 ccagggacag cctggacggc agacggagga tactccactc aggataagac tctggtcaga
      961 aagcccaccg tgatctctgg cgtcacgcaa aacccggtgg gaactggtgc gacggcgttc
     1021 acgaccctgg acacggagtg ggatctatat cccatcgaca cgatctccga tttgggcacc
     1081 cacagttttg gaggaggagg ccagtctgtc agcgctccca cgatccaggc atccaatatc
     1141 gtggcatatc cctccaacga tgaaatcgcg ctggaatgga cggccggaaa tggcagccgc
     1201 agaatagtca aaatcaacgc cagcaacagc ttcacggccc ccgcggatgg aactcagccg
     1261 accgcgaatc ctgtgtattc cggctcggga gaacaggtta tcttcgatgg ggccacccaa
     1321 atcattgaag gcactccctt caatggctgc ctggtatcag gcctgcagcc caacaccact
     1381 tactggttca gaatttatga atacaatgga actgggatca cgacccgctt tctgactact
     1441 tccgctccgg acaatcccag cccggccacc acatccaata ccaccggaat agggtattat
     1501 gccggaataa gtggttatgg ctcaagcatc aagagcagcc ttcatgccct gctcaggacc
     1561 acccacacca cgcagtattc ctatgacgcg ctctggaccc aacttcccta cacagatgaa
     1621 gatccagcca ataccaataa cgttatcgag ctctacacag gctggagcgt tcccaaaaac
     1681 cattatggtg gagggaccac agaatggaac cgcgagcata cctggagcaa aagtcacggc
     1741 gattttggcg agactagacc tgcggggacc gaccttcacc acatgcggcc ctgcgatgcc
     1801 acggtcaatt ccgccaagag caatcgagac tttgatgatg gcggaacggc ttatacagac
     1861 gcgtctccct acccaggtta ttctggggta accggttgct acacatcttc caacacctgg
     1921 gaacccagaa atgaggacaa aggcgacgtg gcccgaatga tcatgtatat ggcggtgcgt
     1981 tatgagggga ccgacacgtc ctatgatctg gaaattgtgg actatacaaa cacggcgccg
     2041 gataacgaac cgctgtatgg caagctctcc accctgctga actggcacgt ccaggaccct
     2101 cccgatgcca gagaaagaca gcgcaacgac cggatatttg agcgtcaggg caaccgtaat
     2161 ccctttattg actatcccag ctacgcgcaa tatatctgga gccctgttcc cactgggccc
     2221 acaagcttga cgcaaacagg cttcacagcc aactggagca cgcccatctc agccaccaaa
     2281 tactatctgc aggtggccac agacagcctt ttcagcagct ttgtgagtgg ctattccaac
     2341 tatgacgcag ccctttcaaa cgccaaaact atcgctggcc tttccaacac aaatacctat
     2401 tactatcgtc tgagatccta tttcctgagc ggctacagca tgtattctcc ctgggtggcg
     2461 gtcaatctgc ttcccaacgg aaccgcccta atagcagctc agagttcagt ttatgaatac
     2521 gacctgcagg gcgctcagct tcagttaaac cttcaaaacg cctctttctc tgatcccagc
     2581 ctcaatctgg ccaatttcac tctgctgaat gggcccgctg gcctcagcat ccagtctgtc
     2641 caatacctca gctccaactc ggccagattg gtcctggctt tcaatgggag cgattttgat
     2701 gacaaccgtc agatcagcat cctgatcagc cccagcgagt taactcaatc caccgcaatc
     2761 acatcatcca gtctgcagct gatcgcctat gtggaaacgc tcctgagcat agatctgcag
     2821 tccggctctt tggtcctgaa tattatccct gtttcccagg caagctcata caggatattt
     2881 ggagctgccg atccttatga tgatttcatc aatatcagcg ctcagggcag ctttgaggtc
     2941 ctggtcccca cgcgctggac cactccctca cccacagcgc cgcgagcttt ttataaagcg
     3001 gtgggaataa gagattagag gtcctggaaa tgagcccaaa ccgcagtaaa acacagcatc
     3061 tgcccttcgc catagatggc gtggaggcct tcaaccagct gctccccgag atgatgaaaa
     3121 acctgttgca caacctgccg ggcatcgcct accggtgtaa aaacgattcc aagtggacca
     3181 tgctgttcat gtctgaggga tgtttcgaag tgaccggcta ctttcccgag gagattctct
     3241 acaacagctt gatgagctat ggggacatga tatatgagga tgaccgtgca ctggtgaggg
     3301 acgctgtgag tgaaggcgtg accaagaagc gacaataccg tatggaatat cgcatcatcc
     3361 gcaaggacgg gtctctgcgc tgggtctggg aacagggcag tgctgtatac agggaatccg
     3421 gcagccctct ctatctggac ggctttatca cggatgtcac tcatcgccgc gagcttgaga
     3481 ccatgctgaa agaaagggct gatcagctct cagagctgaa ccagatgaag gaccgttttt
     3541 tcagcaccat cgttcatgat ctccagaatc ccgtatattc attcatatcg ctcagcgatt
     3601 tcatctgcca gaactacgat cacttcaagc gcgcggagat catggagttt ctcacccaga
     3661 tccgcgatgc cgcccaaggc atgaatctgc tcctcaacaa cctcatggac tggaccagag
     3721 tgcagactgg taaaatgaag ctgcaaaaag acttctttcg cttgcgggcg gtaattgaag
     3781 acggcctgga aggggctcac gaccaaatca acaagaaaaa cctcaacctc gtgatgagca
     3841 ttccggatga tctgcaacta gagacggacc gccaggtggt cgccagcctg tttcgcaacc
     3901 tgctgtccaa cgcgatcaag tattctcaga cccgtgggaa gattatcatc gcggccctga
     3961 ctacagagca agatcttcag cttagcgtcc aggacgaagg agtgggaatt cccagagcgg
     4021 aactgccgga cctctttaaa atggatcggg attatcgccg tccaggcacc atcaatgaaa
     4081 gcgggacggg gctgggattg ttgctggtca aggaattgat ggaccttctg gctggcaggg
     4141 tggaagtgca aagccgggtg aataaaggca ccaaggtcac cctgtttctg cccatcgagc
     4201 tcaagcactt aagctgaaat cagttatgtc cagccaggtg gctggcgtca aatctcttcc
     4261 gcgtcctgcc cgtaaatctg tttgaagcgt tcgttgtcga tctgattggg cacaccttca
     4321 aagaccactt tgccatcctt gagggccagc acccggttgc cgtaacgcct tgccagagtg
     4381 aggaaatgga ggctgcagat cacagtgatc ccctcccggt tgatctcacc aaggtattgc
     4441 atgatgctgt ctgcagtggc ggggtccaga ctggccacgg gttcatcggc cagcacgatc
     4501 tgaggttcct gcatcaaagc tctggcgatt cctacccgct gctgctgacc tccagaaagc
     4561 tgtttcacct gcctgcgggc catatctttg aggcccaccc gttccagatt gcgataggct
     4621 gtttccaggt ctgtggaatt gaatccactc agggccccat ggtaacccag acggcccgtg
     4681 agcacgttgg tgagcaccga gagattcttc accagattaa actgctgaaa caccatccca
     4741 atccgccgtc tgtaatgcct gaggttcgcg ccgttaagac ctcggatgga agagccatta
     4801 aagaggatat ccccggagct gggttctatc agccggttca cacatctcag gagcgtggac
     4861 ttgccgctgc ccgaaagccc taaaaggatg atgaagtctc cctgccgcac ttcaaaactg
     4921 acgtcatcga tggcaaaata gcggccatcg tagctctttt tgagcgactt gatctccagc
     4981 tgcggtacca acgtcaattc agaaaactcg aggggtccag cttcatcagc tgcagggttt
     5041 tacgcaccac gtcgtaatcc gcgtcggtgg cgtactgcaa gccgtcgata tcatagaggg
     5101 agcgcagaat gtctccgcct ttggcagagg cggcaaactc gctcaaagcc ttcactacct
     5161 tggcttccag ttcttggggc atattcttgc ggaaggtgac cgtgtcgttg gggatccaat
     5221 ccgtgaaggc aatggggatt atcttttcca gcacgtccgg cttggtgtca agaagctttt
     5281 cacgcgcgtc tttgggcagt ccactgctgt ccggtggaga ccagtaggtg cagcctacat
     5341 ccgcggtccc gctgtaaact gccaggatgg cggcgttgtg agagcccgtg aagcgcgtct
     5401 gggaaggctc gatgccctgt tctttgagca tggcggaggg gtagatgtat cctgaggtgg
     5461 aagcggcgtc ggtgtaggcc actactttgc cctgaatatc ttcgatagtc ttgatggggc
     5521 tgtcggccct ggccacaaac tggccccggt attgcttgag accgttgcgg acggtcataa
     5581 acttcacttc ggcgtcatac ttctgtttgg caagcacata ggcgaaagtg ggcagccagg
     5641 caatatccgc ttgatagctt cccagcgctt ctatgacggc cgcgtaactg gtgggaaccg
     5701 ccactttgaa gtaatagccc gtctcagcct gcagggcttt ggcaatggct tcgccgctgg
     5761 ctactacctt gccggcttcc agcgagggca caaaatacat cttgatagga ttgcgtttgc
     5821 tgcccagggc cttgttggcc ttgtctatcc tgccggattc actcatgcag gcgctggtaa
     5881 gcacaaacaa caaaacaatg attgtaataa gctttatacg catggctcga tctcctttga
     5941 tcgctcaggg gatgactgga ggcaataaat cacaagcacg ctcagcagtc aaacaaattc
     6001 tccggggaac acccaaaacc actcaaaccc gatgccatcc atcgaagagc gggtccacga
     6061 tcaggagctc cctcgcgagt tgcctggcca gctcactggc gctcagacca tcgaggctga
     6121 tgccgtcgtc attgaacatc gagcctggca ggataagcgt ttgcttgggt tgcgaatcat
     6181 gttggcggca gatgtcctca gctgtcaaaa gtccgctcac gctgatgtga gggcccatga
     6241 agaggtttct gatctcacgg ttgtggagct gggatttatc cagagacgcg ctcaggcggg
     6301 tcacgattct tgttatttcc tgggtcgcgg ccctggaatg caagagcaga taactggcgg
     6361 gatgcttatc cagttccgtg atgaaggctt ttctgcgctt gctaaagccc tgccgcagaa
     6421 ggctgagcaa gccgatcccg ttttcggcct gaggataatc ctgatagtat tggggaggag
     6481 gcaaacgctt gccagacagc acataaaact catcggccgc atagaggata tctcccctgt
     6541 ccagctcacg cagtttatcc agttgtttca ggagggttgt ggcggataat ctgtcaaagg
     6601 gtttgaggtc tggcaagcct tgtctgaaac gggttaagcc taccggcacc actccaatgg
     6661 acagggtagc caggctggga cgcaggaggt ctttaaccgt cctgttcagc tcttccccgt
     6721 cgttgtaacc cggcacacag acaatctggg tgtggtaggc tattcctgct ctgctcaatc
     6781 gcttgagcac tctcagcaga tcaaactcct gtttataacc catcatcctc tgccggagat
     6841 ctttagagct ggtgtgcacc gaaatataga gcgggctgat gtgctgtgtg agcacgcgtt
     6901 tgatctctgc ctcgctgaga ttggtgaggg tgatgtagtt gccgtaatcc ctcgagtaga
     6961 ggtagtcgtc atcctttaca tacaagctgg aacgcagacc cggaggcatc tggtctataa
     7021 agcagaacac gcagtgattg tgacagcgtt taacgggttt ggggtccgtc tccaggccca
     7081 ggggtaaaaa gctatcacgg aggatcgtaa accgtcgctt tttgccatcc gcggcttcga
     7141 tggagacatc aagctgataa tcagaggagt aatactccag gtccatctgg tcgttgatgg
     7201 agtgtccgtt tatccgtaaa accctgtcac ctggctggat atcataacga ctggccaggc
     7261 cagcgggatc aactgagacg atcttgatgc tcaatctgct ttccctggcg gcaggggctc
     7321 aggtattgcg gatcctgtcg tagcggaact tgaagcgctt gagcccctca aagatggtcc
     7381 gcgccaggcg ttcctgatat tggctgtttg ccagtttttg ttcttcatcc gggttgctga
     7441 tgaagccgat ctcgatcaat acggctggca tgaacgcccc tctcagcaca tagaagttgg
     7501 cctgtttgac cccccggtcg aagctcttgg tgcctgccac cagattctgc tgaatgagca
     7561 gggccatatt attggagttt tccaggtgtt ccgtctggct caggtcgctg agaatgaagg
     7621 ccagcgcgtc atacttggcg cgttcagcct tgccgccttc atattcttcc accacgtcgt
     7681 tttccagagc ctccacagcc cgggcgtcag aagtcagggc ggtggaaagg tagtaggttt
     7741 ccaggccgga agcggcgcgg tttttggagg cgttggtatg cacgctcaca aagaggtccg
     7801 ccctgtgctc attggcaaac ttggtgcggt ttttgagcgt gataaagcga tcatcgttcc
     7861 gggtcatcaa gactgtgatg cccagctcct tttccagcat ctgcttgagc ttgaggcaca
     7921 cgttcagatt tacttccttt tcccgcagtt tcagcttttt acctaccgcg cccgggtcgt
     7981 tgcctccatg gccaggatcc agaactatca ccttcaccga gcggtcttta gggcgctcga
     8041 tctgcagaac agtgcctttc atctccatgt ccttaaaatg ggtgggaaga tgttctgtga
     8101 caaacacgga aggcagataa aactggttgc ccgagcgggt cacattgagg tggaggttcc
     8161 aggattcgtt ttcaaagctg taaaaaggag agccgatcag aaaaatgaac tgcttgccat
     8221 tcagaaacag atgcagacgg tgatcgccgc gttcctgctt cacgatggaa ttgaacacct
     8281 gattgaaagc gtcaaaatcc acgtatagct tttcggcgat cttggtttcc ggcaaagcga
     8341 cggtcttgcg ttcattcttg aaataaacgc tgatgtccgc ccaggccagt gaaagagaca
     8401 ttatgaacag ccaggctgtg agtagtgttt tagtgttttt cata
//
LOCUS       DAOSFN010000115         8439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_7185_length_8439_cov_7.555224, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000115 DAOSFN010000000
VERSION     DAOSFN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7185_length_8439_cov_7.555224"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            404..1600
                     /locus_tag="PLO35_07525"
     CDS             404..1600
                     /locus_tag="PLO35_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOH79557.1"
                     /translation="MKKSMLAILMLALLFTLLSAQKVVNINGTEIDLEGLEELEKLED
                     MDSGTQNVKIMINKEFNPMTNDGSGYFGIYVDDLNFPKAQKLSYNQMIGVLITGVVQG
                     SPAWEARLQEDDIIMNINDMPVYNKEEFDRLRKNMRPGDALKINLWRSGGEVPVDLVL
                     GSREAPSAPGQAGKPAKKRNSVGYGGGGWTPTWLQTDMTEINALMTSIGLEAYNEDGV
                     LTQGFAGKGHVGKGLFLGFQVNDFNDKRNIRDNLSDGGPYDVTVDYNLSLVGATIDKR
                     FAIAPWLVTSIGVMLGGGSHDIEFTRVNHHYDWPDSLQNVTQGNFNSTLHRGYLMVQP
                     RAELMFRILPWLGLRAEAGYVYTYAPYSGWRAKGAEDGDMVEVQGSPDTKLEGFSIGI
                     GPWFGF"
     gene            2100..3455
                     /gene="der"
                     /locus_tag="PLO35_07530"
     CDS             2100..3455
                     /gene="der"
                     /locus_tag="PLO35_07530"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOH79558.1"
                     /translation="MRKYIVSIVGRPNVGKSTLFNRLCRKRSAIVDFEAGVTRDRKYE
                     DVEWNGKVFKLVDTGGIVFDSSEAMDRMIRHQALLAIEESDLIIFMVDAMTGSTDIDI
                     EIARILYPHRDKVLLAANKADNEKYEWELFDFLQLGFGDAFPISAAQGRNTGNFLDEL
                     ISMIPKTADAYDEAPKDAPTRIAVVGKPNVGKSSIVNLMLGDDKQIVTEIPGTTRDSI
                     DSAFRYHSRDYVLVDTAGLRRKTKVNYGVEYFSTMRTVEAVDRCDIVVLVLTADEPIS
                     QQDVKIASYARRKLKEIMVVFNKWDLVDKDNSSTGKFLADLHEQMPFLQFAPVQFISA
                     KTGQRINRVMETVVKIEDEAQKRVSTSDLNKFMETVIARRPPTHPKGKHVKILYITQA
                     SIKPPTFVFFCNQANLVTENYRRFLHNQIREMFAFEGVSIRLIFKGRKGDNQDTPNEL
                     R"
     gene            3442..4080
                     /gene="plsY"
                     /locus_tag="PLO35_07535"
     CDS             3442..4080
                     /gene="plsY"
                     /locus_tag="PLO35_07535"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOH79559.1"
                     /translation="MSCASFWSCPFRIMAIAYLVGSIPFGWLMGKLFFKEDIRQAGSG
                     NIGATNALRRFGTVPGIIVLLLDMLKGFAVAWFAKEVFMPGSYWIPIAGALVIIGHMF
                     PIWLNFKGGKGVATAAGVFIALTLWPVLIALATFLITVIISRYVSLGSILAALALMVS
                     SIVQQSLQTWDLPMLVFITLLVIMIIFKHIPNIKRLLAGHENRLQFSRKGNR"
     gene            4080..5456
                     /gene="purF"
                     /locus_tag="PLO35_07540"
     CDS             4080..5456
                     /gene="purF"
                     /locus_tag="PLO35_07540"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOH79560.1"
                     /translation="MCGIMGVYGSPQAASLAALGMFAEQHRGQESCGMAVSDGKVIRL
                     RKKMGLVKEVFRDEKLDLLPGSIAIGHVRYPTKGSATQFNSQPHLLETLFGPSHALAS
                     NGDIVNYYEVRRKLEAEGVYFNSDNDGELLLRYIAWQIQSNRQGITDAIRLLMKEIKG
                     AYSSVLLIRDALYMFRDPLSIRPMVWGITASGAVVVASESCALATMGVSVYKEVPPAG
                     IIRVNADGITVLENDPNLFRAIDSERHCVFEHIYFSRPDSHHFDENVYEVRQRIGAQL
                     AIKDADLDADLVVPVPDSSNFIAMGYASAKKVPLAMGLIRNHYVGRTFIKPEQTIRDE
                     SVRQKFNVLPNFFNGKRIVLVDDSIVRGTTIGKIVKLIRDAGASEVHLRIGSPQVRYS
                     CYYGIDTPDSDELVANRLSLDEICRQSGADSLRHLDSDGLKRCVCHPQNYCYACFDGN
                     YPLGVAHA"
     gene            5449..6444
                     /gene="rfaE1"
                     /locus_tag="PLO35_07545"
     CDS             5449..6444
                     /gene="rfaE1"
                     /locus_tag="PLO35_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOH79561.1"
                     /translation="MPENKQGKDQYTRIITAFAGLKVMVLGDLMLDHYIWGKVERISP
                     EAPVPVLDVSQEEYRLGGAANVALNLKALGAEVCLIGVHGKDAAARILRSKLSKAGIS
                     TDALLALKDRRTTLKTRIGSRTQQIVRVDYEHSSLLSAEIEAAIVAILEAQLPGCDAL
                     IIEDYDKGLLTPGLISEAIRLCRKAAIPVAVDPKLRHFFHYNGVDIFKPNYSELQRSL
                     GKSFEADEQFFKAASETRTRMNARFLVLTRGARGMYVFSDGPVKHLPTVAREVFDVSG
                     AGDTVISVLTLAWICGAEIDLAANLANHAAGVVCGKLGTASATSKEILDSIHEQR"
     gene            6431..6910
                     /gene="rfaE2"
                     /locus_tag="PLO35_07550"
     CDS             6431..6910
                     /gene="rfaE2"
                     /locus_tag="PLO35_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOH79562.1"
                     /translation="MNRDKIFSLNELKDVLGLYRNTGGRIVFTNGCFDIMHAGHAQYL
                     EEARAMGDVLIVAMNSDASVRRLKGPKRPVIGQQDRALMLAALESVDYVVIFEEDTPY
                     EVINILEPDLLVKGGDWTPDQIVGADIVLARGGEVKSLPFRPGLSTSSIIERIRESM"
     gene            6907..7341
                     /locus_tag="PLO35_07555"
     CDS             6907..7341
                     /locus_tag="PLO35_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOH79563.1"
                     /translation="MSGFDDNATEDIGKVIQVSGSHAKVEIQRGGGCGSCAMRGFCFK
                     KGEPAVFDVQTELPLKEGDLVSLEIAPSVRVLSALLVFGLPLSLLFIAFIVASLYFTE
                     LVSVLLAFGAMALSFAIVGCLDRRFGKNLKISVGGIHDHKAQ"
     gene            7322..7678
                     /gene="folB"
                     /locus_tag="PLO35_07560"
     CDS             7322..7678
                     /gene="folB"
                     /locus_tag="PLO35_07560"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOH79564.1"
                     /translation="MIIKLNEMVFYGFHGVHDEERRLGQRFIVSAILHTDPALDSRIR
                     NLEDTVDYTKVYDDIREIMETHQFQLLEVCANTVADKLLLDYPLLTAVELRIQKPSVP
                     IQGSLSSVEVELCRKR"
     gene            7663..8175
                     /gene="folK"
                     /locus_tag="PLO35_07565"
     CDS             7663..8175
                     /gene="folK"
                     /locus_tag="PLO35_07565"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOH79565.1"
                     /translation="MPKALTKVWLSLGANLGYPRLQLAQAHKLLSARGIRILRKSALY
                     DTAPWGKTDQPDFVNQVLEVETALSAAELLHAIVAIEMDMGRVRTEKWGPRLIDIDIL
                     LYADQIIETAELMIPHPRFHLREFCLGPLCELEPDLLHPVLKKSMQELREALNTGTGG
                     PGPTNPKEES"
     gene            8175..>8439
                     /locus_tag="PLO35_07570"
     CDS             8175..>8439
                     /locus_tag="PLO35_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOH79566.1"
                     /translation="MRPLAAIILAAGKGTRMKSSRAKVTFPIAGKPMVQRVVNTALAV
                     DCNKIAIVIGYLKESVIGCLEEDDRLVFVEQTEQLGTGHAVMCT"
BASE COUNT         1996 a         2239 c         2312 g         1892 t
ORIGIN      
        1 gccaggctga gtaagattgc cagaatatag attgtgcgtc tcatgaatgt atcctttgtg
       61 atccgtcttg gggctgtatt tcaatcttaa gaccgttgca catagtagta acactggctt
      121 cagccggtcg tagcactgac tttagtcggt gcgtgcatgt tattcaacga tccagtctat
      181 ataataaagc atgtttcatt ccctgacgaa tctgaagtcg cggaccgcct tcccggtcta
      241 tatcaagagt catatcaaat tgatataacc cttgctggac aagtaacaga ccaggtgtta
      301 taagtggttg tatatcatgc ttttaacact atcagcagaa ttggctcgcg ctttgcatct
      361 gcctaaggat acataaacgc aattcaataa cctggaggtt aaaatgaaaa agtcaatgct
      421 cgcgatcctg atgctggccc tgttgttcac gctgctcagc gcgcagaaag tggtgaatat
      481 caacgggacc gagatcgatc tggaagggtt ggaagaactt gagaagcttg aggatatgga
      541 ttccggcacc cagaatgtca agatcatgat caacaaggaa ttcaacccca tgaccaacga
      601 cggctcgggc tattttggca tctatgtgga cgacctgaac tttcctaaag ctcaaaaact
      661 gagctacaat cagatgattg gtgtattgat cacgggggtg gtgcagggtt ctcccgcttg
      721 ggaagcccgc ctgcaggaag acgatatcat catgaacatc aatgatatgc ccgtgtataa
      781 caaagaagaa tttgaccggc tgcgtaaaaa catgcgcccc ggtgacgctc tgaaaatcaa
      841 tctctggcgt tccggtggcg aagtaccggt ggacctggtc ctcggcagcc gagaagctcc
      901 cagcgctcct ggccaggcag gtaaacccgc caaaaagaga aactccgtgg gttatggcgg
      961 tggcggttgg acccccacct ggctgcagac agatatgaca gaaatcaacg ctttgatgac
     1021 cagtataggc ctggaagctt acaacgaaga cggcgtcctg acgcagggat tcgctgggaa
     1081 gggacacgtc ggcaaaggcc tatttctggg ctttcaggtc aatgacttca acgacaaacg
     1141 caatatcaga gacaacctga gtgatggcgg tccctacgat gtaacggtcg attacaatct
     1201 gtcgctggtc ggcgctacga tcgacaagcg tttcgcgatc gccccctggc tggttacatc
     1261 gattggcgtg atgctgggtg gaggcagtca tgatattgag tttacccgcg tcaatcatca
     1321 ttacgactgg ccggattccc tccaaaacgt cactcagggc aatttcaatt ccaccctcca
     1381 tcgcggttac ctgatggtcc agccccgggc tgaactgatg ttccgcatcc ttccctggct
     1441 gggtctcagg gccgaagccg gttacgtcta tacctacgct ccctacagcg gctggagggc
     1501 caagggcgct gaggatggcg acatggtgga agtacagggt tctcctgata ccaaactgga
     1561 aggtttttcc atcggaatcg gaccctggtt cggtttctaa aacacgcccc ctactctcaa
     1621 gcataaacga aataccccgg atcacccggt ccggggtatt tatttggtgt gtagaggttc
     1681 tggaagatta ggtatttccc gctgattagg tatttcccgc tgattacgca gattttcccg
     1741 cagattacgc agatggtcca aaggtaggcg cggaagcgaa cgctggtttg tcgcaaccct
     1801 ctcaaccctc tcaaccctcg caaccttcac aacttctcaa ccctgcaact atctgcgtga
     1861 tctgcgcttc aatctgcgta atctgcggga aataatgcgt aagctgcggg aaaatgcctg
     1921 ccgcctcaac tgaatattga gagtggtcct ggggagttta ggatacctcc tcaaagccgc
     1981 aaacccgatc acgaattcaa taggattgag tgctgctccg cttcagtgat aagccattga
     2041 caaaatacag gcttgaaaaa gacttgcaca aaacccatca ggggaaaact ggataataaa
     2101 tgcgaaaata catcgtctcc atcgtgggca ggcccaacgt gggcaagtcc accctattca
     2161 accgcttgtg ccgcaagcgt tcagccatcg tggatttcga ggctggagtc acccgcgacc
     2221 gcaaatatga ggacgtcgag tggaacggca aggtgttcaa actcgtggac accggcggga
     2281 tcgtgttcga cagctccgag gccatggaca ggatgatccg tcatcaagca ctcctggcca
     2341 tcgaagagag cgatctgatc atctttatgg tggatgccat gacaggcagc accgatatcg
     2401 acattgagat cgccagaatc ctgtatccgc accgggataa agtgctcctg gcagccaata
     2461 aggccgacaa cgaaaagtat gagtgggaac tctttgactt tctgcagctt ggctttggag
     2521 acgcctttcc gatctcggcc gctcagggac gcaataccgg aaactttctg gatgaactga
     2581 tctccatgat ccccaagacc gccgatgcct atgatgaagc tcccaaggac gcccccaccc
     2641 gtattgccgt ggtgggaaag ccaaacgtgg gcaagagctc catcgtcaac ctcatgctgg
     2701 gggacgacaa gcagatcgtc acggaaattc caggcaccac cagagattcc atcgacagcg
     2761 ccttccgtta tcatagccgc gactatgtcc tggtggacac ggctggcttg agacgcaaga
     2821 ccaaggttaa ttacggggtg gaatactttt ccaccatgcg gaccgtggaa gcggtggaca
     2881 ggtgcgacat tgtggtgctg gtgctcactg ccgatgagcc gatctcgcag caggacgtga
     2941 agatagcctc ctatgccaga cgcaagctca aagagatcat ggtggtcttt aacaagtggg
     3001 acctcgtgga caaagacaac agcagcaccg gcaagttttt ggccgatctg catgaacaga
     3061 tgccttttct gcagttcgcg ccggtgcagt ttatctctgc caaaaccggc cagcgcatca
     3121 accgcgtcat ggaaacggtg gtcaagatcg aggatgaagc ccagaaacgg gtttccacct
     3181 cagacctcaa caagtttatg gagacggtaa tcgcgcgccg gcctcccacc caccccaaag
     3241 gcaagcacgt caagatcttg tatattaccc aggcatcgat caaaccgccc actttcgtgt
     3301 tcttctgcaa ccaggccaat ctggtaaccg agaactaccg gcgctttttg cacaatcaga
     3361 tccgcgagat gtttgccttt gagggagtga gcatcaggct catcttcaag ggccgcaaag
     3421 gtgacaacca ggatacgccc aatgagttgc gctagtttct ggagctgtcc cttccggatc
     3481 atggcaatcg cctatctggt gggcagcatc ccctttggct ggttgatggg caagctcttt
     3541 ttcaaagaag acatccgtca ggctggtagt ggcaacatcg gcgccaccaa cgctttacgg
     3601 cgctttggaa ccgtgccggg gatcatcgtg ctgctgctcg atatgctcaa gggcttcgcg
     3661 gtagcctggt ttgccaaaga ggtttttatg cctggcagct actggattcc gatcgccggg
     3721 gctttggtga tcatcgggca catgtttccc atctggctca acttcaaggg cggcaaggga
     3781 gtagccacgg ccgcgggggt ctttatcgct ctcacgctct ggccggtcct gatcgcgctg
     3841 gccacctttc tgatcactgt gatcatcagc cgctatgttt ccctgggatc tatcctggcg
     3901 gccctggccc tgatggtctc cagcatcgtc caacaatccc tccagacctg ggaccttccc
     3961 atgctggtgt tcatcaccct gctggttatc atgatcattt tcaagcatat ccccaatatc
     4021 aagaggctgt tggctggcca tgaaaaccgc ctccaattca gcaggaaggg caaccgctga
     4081 tgtgtgggat catgggagtc tatggctccc ctcaggccgc atcactggcc gcattgggga
     4141 tgtttgccga acagcatcgc ggccaggaaa gctgcggtat ggctgtcagc gatggcaagg
     4201 tgatcaggct gcgcaagaaa atgggcctgg tgaaggaagt gtttcgagat gaaaagcttg
     4261 acctgcttcc aggcagtatc gccatcgggc atgtgcgcta tcccaccaag ggcagcgcca
     4321 ctcaattcaa ttcccagccc cacctgctgg aaacactttt tggcccttcg cacgcgctgg
     4381 ccagcaatgg cgacatcgtt aactactacg aggtgaggcg caagctggaa gcggaaggcg
     4441 tctattttaa cagcgacaac gacggcgaac tgctgctgcg ctacatcgcc tggcagattc
     4501 aaagcaaccg acaggggatc acggacgcta tcagactgct gatgaaagag ataaaaggtg
     4561 cttattccag cgtgctcctg atccgcgacg cgctctatat gttcagagac ccgctcagca
     4621 tccgtcccat ggtgtgggga atcactgctt caggtgcggt ggtggtggcc tctgaaagct
     4681 gcgctctggc aactatgggc gtgagtgttt ataaagaagt tccgcccgcg gggattatca
     4741 gagttaatgc cgatggaatc accgtgctgg aaaacgatcc caatctcttt cgtgctatcg
     4801 attcggagcg gcactgcgtg tttgaacaca tctatttcag ccgtccggac agtcatcact
     4861 tcgatgaaaa cgtctatgaa gtgcgccaac ggatcggggc ccaactggcc atcaaagacg
     4921 ctgatctgga cgccgatctg gtggtcccgg tgcccgactc ttccaacttt attgccatgg
     4981 gctatgccag cgccaaaaag gtccctctgg ccatgggctt gatccgtaac cattatgtgg
     5041 gacgtacatt tatcaaaccg gagcagacca tccgggatga aagcgtgcgc cagaagttta
     5101 acgtgctgcc caatttcttc aatggcaagc ggatcgtgtt ggtggacgat tccatcgtgc
     5161 gcggcaccac gatcggcaag atcgtgaagc tgatcagaga tgccggtgcc agtgaagtgc
     5221 acctgcgcat cggctcgccc caggtgcgct attcctgcta ttatgggatc gacacgcctg
     5281 attctgatga actggtagcc aaccgcctct ctttggatga aatctgccga caaagcggag
     5341 cggacagcct caggcatctg gattcagatg gcctcaagcg ctgcgtttgc cacccccaga
     5401 actattgtta cgcctgtttt gacgggaatt atcctctggg agtggcacat gcctgaaaac
     5461 aagcagggca aagatcaata caccaggatt attacagctt tcgccggtct caaggtgatg
     5521 gtgttgggcg atttgatgct ggaccactat atctggggaa aggtggaacg catctctccc
     5581 gaagcccccg tcccggttct ggatgtcagc caggaggaat accgtctggg aggtgccgcc
     5641 aacgtggccc tcaacctcaa agccctggga gccgaagtct gcctgatcgg agtccatggc
     5701 aaggacgccg ccgccagaat tttgcgttca aagctaagca aggcaggcat ttccactgat
     5761 gctctgcttg cgctgaaaga ccgccgcact actctgaaga ctcggattgg cagccgcacc
     5821 cagcagatcg tgagagtgga ttatgaacac agctcgctcc tgagcgccga gatcgaagcg
     5881 gccatcgtcg ccattctgga agcacagctg cccggctgcg acgctctcat catcgaagac
     5941 tatgataaag gcctcctcac ccctggattg atctcggaag ccatcaggct ttgccggaag
     6001 gccgccatcc cggtagccgt ggatcccaaa ctgaggcact ttttccacta taacggggtg
     6061 gacattttca agcctaatta ttctgaactg cagcgcagtc tgggcaagag tttcgaagct
     6121 gatgagcagt ttttcaaagc cgcctcggaa acccgaacca ggatgaacgc acgctttttg
     6181 gtgctcacca ggggagccag aggtatgtat gtgttttcag atggacccgt taaacatctg
     6241 cccaccgtgg ccagagaagt ctttgacgtc tccggagcgg gagacacggt gatcagcgtg
     6301 ctcactctgg cctggatttg cggggcagaa atagacctgg ctgccaacct cgccaatcac
     6361 gccgccggag tggtctgcgg caaacttggc accgccagcg cgacctccaa agagattttg
     6421 gacagcatcc atgaacagag ataagatatt ttccctgaat gaattaaaag acgtcctcgg
     6481 actctataga aataccggtg gccgcatcgt gtttaccaac ggctgcttcg atattatgca
     6541 tgccggacat gcccaatacc ttgaagaagc aagagctatg ggcgacgtcc tgatcgttgc
     6601 gatgaactca gacgcgagtg tcagacgcct gaagggtccc aagcgtcctg tgatcggtca
     6661 gcaagaccgg gccctcatgc tggcggccct ggaatcggta gattatgtcg ttatatttga
     6721 agaagatact ccctatgagg tgatcaatat cttagagcca gacctcctgg taaaaggtgg
     6781 cgactggact cccgatcaga tcgtgggggc ggatattgtg ctggcccggg gcggtgaagt
     6841 gaagagcctt cccttccggc cggggctatc caccagcagc atcatagagc ggatcaggga
     6901 gagcatgtga gcggatttga tgacaacgcc acggaagata tcggcaaagt gatccaggtt
     6961 tcgggctccc acgctaaagt ggaaatccaa cgcggcggcg gttgtggtag ctgtgccatg
     7021 cggggctttt gttttaaaaa gggagaaccg gccgtgtttg acgtgcagac cgaattgccc
     7081 ctcaaggaag gcgatttggt gagcctggaa atcgctccat ctgtccgggt cctctccgcg
     7141 cttctggttt ttgggctgcc gttgagcctg ctttttattg cctttatagt tgccagtctg
     7201 tactttacag agcttgtgag cgtcttgttg gcctttgggg ccatggcttt gagcttcgct
     7261 atcgtgggct gtctggaccg tagatttggc aagaacctaa agatcagcgt gggaggcatc
     7321 catgatcata aagctcaatg agatggtctt ttatggcttc catggagtgc acgacgaaga
     7381 gcgccgtctg ggacagcgtt tcatcgtgag cgccatcctg cacacggatc ccgctctgga
     7441 ttcacgcatc cgcaacctgg aagataccgt agattacacc aaggtgtatg acgacatccg
     7501 tgagatcatg gagacgcatc agtttcagct cctggaggtc tgcgccaata ccgtggcgga
     7561 taagctgctg ctggactatc ctttgctgac agcagtcgag ctgcgcatcc aaaaaccctc
     7621 cgtgcctatc caggggtctt tgagcagcgt cgaggtggag ctgtgccgaa agcgttaacc
     7681 aaagtctggc tgagcctggg tgccaacctt ggatatccca gactgcaact ggcccaggcc
     7741 cacaagttac tatccgccag agggatcagg atactaagga aatccgcctt gtacgacacg
     7801 gccccctggg gaaagaccga tcagcctgat tttgtgaacc aggtcctgga agtggagaca
     7861 gctctcagcg cggcagaact gctacacgcg atcgtagcta ttgagatgga tatggggcgc
     7921 gtccggaccg agaaatgggg cccccgtctg atcgatatcg acatcctcct gtacgctgat
     7981 cagattatcg agacggcgga actgatgatc ccgcatccca gattccatct gcgggagttt
     8041 tgtctgggtc cactctgtga attggaaccg gatttgctgc atccagtctt aaagaaaagc
     8101 atgcaagagc ttagagaagc tttgaataca gggacgggcg ggccaggacc caccaatccc
     8161 aaggaggaat cctgatgaga ccccttgcgg cgattatttt agccgccgga aaaggcaccc
     8221 ggatgaaatc ttcccgggcc aaagtaactt tccccatcgc gggaaaaccc atggtccaga
     8281 gagtggtaaa tacagctctg gctgtggact gcaacaaaat cgcgatagtg attggctatc
     8341 tcaaagagag tgtgatcgga tgtctggaag aagacgatcg gctcgtgttt gtggaacaga
     8401 ctgaacagct cgggaccggc catgccgtca tgtgcactg
//
LOCUS       DAOSFN010000116         8281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_7380_length_8281_cov_8.576830, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000116 DAOSFN010000000
VERSION     DAOSFN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7380_length_8281_cov_8.576830"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1794
                     /locus_tag="PLO35_07575"
     CDS             124..1794
                     /locus_tag="PLO35_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH79567.1"
                     /translation="MKERTYTVLVGGKAGEGVKKAAQVIAGVFCSRGLHACQADDYQS
                     LIKGGHNFSVVTAGAAEIECVHQKWDLLICFDQRSVDEHLARLKTDGSIYYNSDEATC
                     ERGFGLPMNALMKKHYQGPANVSMAAIAIFAAVMRMSKDMLKELIKRQYKRDVSENIT
                     YAYDIYDLITSHTDLLDWDATPLGYRFLSGNQAIILGAWKAGLDMYYGYPMTPASSLL
                     HYAALKQDSLGIQAIHAESELAAVNMALGSALAGCRVAVGSSGGGFALMQEAFSAAGM
                     VEAPLLCVLSSRPGPATGVSTYTAQEDLWFSLFQGHGEFNRIVASPDSISRALSLSAE
                     LLSLSWEFQVPSILLTEKQLSEGLTDLQHSSEPLPEAEPQTGIPGKDYKRYQDNLKGV
                     SPLLFPGSQFAPETVIKWSTHEHLESGLRTDKADEIVLMKDKRQKKAAGISSACSKFQ
                     TFAEYGEGERLIFAYGSTVLELREALKFSALAFRIVAPVYLEPFPLGLEKYRGASAIV
                     VEHSSSGQFAQFLKWKLDIRATTVIKKYDGRPFDPEELSELIREASHA"
     gene            1787..2605
                     /locus_tag="PLO35_07580"
     CDS             1787..2605
                     /locus_tag="PLO35_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH79568.1"
                     /translation="MRDLNTPKANTWCPGCGNFGILNSFKNSIVRLVETGINVDDIVI
                     TCGIGCHGKIFDYLNLSGMYCLHGRALATAQGIKLANPDLKVICFGGDGDSLGEGLEH
                     TLFAAKRNLDVTLILHNNGTYGLTTGQFSPLSQRGYQGLSTPKGSIETPFRPIPLLLE
                     AGATFLARAYSARPEQLCATIVNAVLHKGFSFVEVLQPCVSYNNSYQHYNRIVKELET
                     IPGKPDQALKLATDPDSVFLGVFYQEHKPVFHEELYQNRNPFRDRIPASHRTLP"
     gene            2602..3387
                     /locus_tag="PLO35_07585"
     CDS             2602..3387
                     /locus_tag="PLO35_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH79569.1"
                     /translation="MKKPVQFRRHVWQIVFLGASLTWLAATLFTQRVNIHQACPYAVV
                     CFGAMDAGIINTASTVAVWAVIVGLLIVLSTMWLGRRFCAWICPLGTVQEGLFSLRSK
                     SYKIKRKLPFWYDRKFAWIKYFLLGLTTLLAALTLSWRYMRLCPFVTLARLPGVVWQG
                     AIVLLIILVSSFLVERWWCRFLCPYAALMNLAQKLGQFMGINRSKMKRNLERCNDCGV
                     CVLYCPMNLNLAESEYVQSENCIHCGLCAEVCPKPGTYSEESE"
     gene            3384..3956
                     /locus_tag="PLO35_07590"
     CDS             3384..3956
                     /locus_tag="PLO35_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH79570.1"
                     /translation="MKQAFRIMFIVLLLSVISLQIVTLLSSRQGTRTVQICPTGAISM
                     QGSKAVINSEKCIGCQRCVLGIPVRFPQVRDQVPQVSVSLPQRDSISSAPLPAPPPKQ
                     ADAVKPKDAARSNQPQAIKPSEDKETYQVNSAACIGCQLCVSACPTQAISMVNGKAVI
                     DKEKCINCGICVNGDNNEFQGCPVGAIKAP"
     gene            4120..>4922
                     /locus_tag="PLO35_07595"
     CDS             4120..>4922
                     /locus_tag="PLO35_07595"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOH79571.1"
                     /translation="MRKTVVVSAKRTAIGNFGGVFKDISAVKLGVNVAKAIFQETGVK
                     PELIEEVIVGNVCGAGYGQNPARQIAIHSGIPEHVPAYTVNKVCGSGMKAVALAAMMI
                     GAGEADIILAGGAENMSQIPYASMDARWGGRMGNKELVDLMIRDGLSDIFNDYHMGIT
                     AENLAAKYQISREEQDAFAASSQNKTETAMNTGRFQDEIVPIVIPQKKGDPLVIDRDE
                     MPRPGVTAASLGKLRPAFKADGSVTAANSSGINDGAALLLLMSDEKAREL"
     assembly_gap    4923..5022
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5023..5181
                     /locus_tag="PLO35_07600"
     CDS             <5023..5181
                     /locus_tag="PLO35_07600"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOH79572.1"
                     /translation="NGGAIALGHPIGASGARIIVTLLHEMKKRQVHKGLASLCIGGGM
                     GIATLWEM"
     gene            5189..6433
                     /locus_tag="PLO35_07605"
     CDS             5189..6433
                     /locus_tag="PLO35_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH79573.1"
                     /translation="MADKLKLDKSMALFAEAQTLVPGGVAGIRRPYNFVVGEYPIFFE
                     EGKGGRITDVDGNEYIDYLCAYGPISIGYREDEIDDAVIEQIKNKGFCFSLTQAIQNK
                     LVRKLREVIPCCEMAAIVKTGSDATTIAIRTARAYTGRVKIARCGYHGWHDWCVEVKG
                     GIPEKLYEDILEFHYNDLDQVESICKQHGDDLAAIIVTPVGHPLAAEVEMPKPGYLEG
                     LRALADKYGCVLIFDEIRSGFRVSLGGAQAYFGVTPDLATFGKAMANGYAIAALVGKS
                     EIMNVMADKVFLSSTFFPNSDAQVAALKTIEILERDKVTDVIAAKGYKFAAEVEAVVK
                     ESGIPARFSGAPWMPFITFSKDQNGLYKKLRNEFYTQLIRRGVFLQPYHHGYICYRHT
                     DEDLAYTVGAIKASLADLKSLC"
     gene            6446..8056
                     /locus_tag="PLO35_07610"
     CDS             6446..8056
                     /locus_tag="PLO35_07610"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOH79574.1"
                     /translation="MAKYIFVMGGVLSSLGKGIATATIGLLLKEMGYRVSLQKFDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFVDVALTRDSSCSAGQVYETVLLNE
                     RKGVYLGKTVQVIPHVSNEIKRRILKQNDNFDVIITEIGGTVGDIESLPFLESVRQLR
                     LELGTRNCLCVLLTYVPYIKTAGELKTKPSQHSAYKLREIGIQPDILLCRSEKQFDDE
                     IYSKIALFTNVPKSNVFNAIDVKCVYEVPIVYQKAGLPKIVCDHFELEYREPKLDSWE
                     RFLKASYSSSPKLTIAVCGKYVKHQDAYKSVEEALLHAAAYHGHKLAIKWVDSEREFC
                     NEDLDKELDGVQGILVPGGFGVRGIEGKISIARYARIKNIPYLGLCLGLHVAVIEFSR
                     NVCGLKNADSTEFDELNEYPVVHMMEDQQYQELVGGTMRLGAYPCKLEPGSLVHKAYE
                     ADLIFERHRHRYEVNNQYRDLLGSKGLKITGSSPDNLLVEIMELETNDFHLGVQFHPE
                     FKSRPQRPHPLFRDFEAAAIKYAIRKGH"
BASE COUNT         2009 a         2038 c         2224 g         1910 t
ORIGIN      
        1 tgagctgggt tatctgaatg tttgacacgc gttttcccgc gttggaagct tggataaggg
       61 ttttctattg acagcggagg cttgtatttt gaaattgcag caacgacaat aaatggagtg
      121 ttcgtgaaag aaagaacata tacagtgctt gtaggtggaa aggccggcga aggagtcaag
      181 aaggccgctc aggttatcgc tggagtcttc tgctcccgcg gtctgcatgc ctgccaggct
      241 gatgattatc aaagcctcat caaaggcggg cacaatttca gtgttgtgac cgctggcgct
      301 gcggagattg aatgcgtcca tcaaaagtgg gacctgctga tctgctttga tcaacgcagt
      361 gtggatgagc atctcgcgcg cctcaagacg gatggatcga tatactataa ctcagacgaa
      421 gccacttgcg agcggggatt cggcttgccg atgaacgctt tgatgaagaa acattatcaa
      481 gggccagcca atgtcagcat ggctgctatt gccatcttcg cggcagtaat gcggatgagc
      541 aaagacatgc tgaaagagct tataaagaga cagtataaac gtgatgtgtc agaaaacatt
      601 acatatgcgt atgatatata tgatcttata acaagtcata cagacctgct ggactgggat
      661 gccactcctc ttggatatcg tttcttatcg ggaaaccagg ccatcatcct gggagcctgg
      721 aaagcaggac tggacatgta ttatggttat cccatgaccc cggcttcttc attgctccac
      781 tacgcggctt tgaagcagga cagtctgggt atccaggcga tccatgccga aagtgaactg
      841 gccgcggtaa atatggccct gggttccgct ctggcgggat gccgtgtcgc cgttggcagc
      901 agtggaggcg gcttcgctct gatgcaggaa gcattttctg ccgccggaat ggtcgaagcg
      961 ccgcttttgt gtgtcctttc ttccaggccc ggtcccgcga ctggggtatc tacttacacg
     1021 gcccaggaag atctctggtt ttcgctgttt caggggcatg gtgagttcaa ccggattgtg
     1081 gcctcccctg attctatctc aagggcccta agtctttccg cggaattgct ttccctatcc
     1141 tgggaatttc aggtcccctc catcctgctc accgaaaaac aactttcgga agggttgacg
     1201 gatctgcagc attccagcga gccgctgcca gaagctgaac cccaaaccgg aatacccgga
     1261 aaggactata aacgctacca ggataacctg aaaggagtat cgccacttct gttcccaggc
     1321 tcgcaattcg ctccggaaac cgtgatcaag tggagcaccc atgagcatct ggagagcggc
     1381 ttgcggaccg ataaagccga tgagatcgtg ttgatgaaag ataagcggca aaaaaaggcg
     1441 gccgggattt catccgcctg ctcaaaattt cagacctttg ccgaatatgg agagggtgag
     1501 aggctgattt ttgcctacgg atcgacggtt ttggaactgc gcgaagctct gaagttcagc
     1561 gctctggcct tcaggattgt ggcccctgtc tatctggagc cctttccact gggattggaa
     1621 aagtaccgcg gcgccagcgc gatcgtggtg gaacacagct cctcgggtca atttgcccag
     1681 tttctgaagt ggaagctgga tatcagggca acgactgtga tcaaaaaata cgacggccgc
     1741 ccatttgacc ccgaagaatt gagcgaactg ataagggagg ccagccatgc gtgatctgaa
     1801 cacccccaaa gccaatacct ggtgcccggg ttgcggtaac tttggcattc taaattcctt
     1861 taaaaacagc attgtcagac tggtggagac cggcatcaat gtggatgata tcgtgatcac
     1921 ctgcggaatt ggctgccatg gcaaaatctt tgactatctc aatcttagcg gcatgtattg
     1981 tctgcacgga cgcgctttgg ccaccgctca gggcatcaaa ctggccaatc ccgatctcaa
     2041 ggtcatttgc tttggagggg atggtgacag tctgggtgag ggcttagagc acactctttt
     2101 cgcggcaaag cgcaacctgg acgtcactct tatcctgcac aacaacggga cctatggact
     2161 cacgacgggg caattttctc ccctttccca aaggggttat cagggccttt caactcccaa
     2221 aggcagcata gaaacgccct tcaggcctat tccgctgctt ttggaagcgg gtgcaacctt
     2281 tctggccaga gcctacagcg ctcgtccgga acagctctgc gccacgatcg tcaacgccgt
     2341 tttgcacaag ggcttctcct ttgtggaggt gctgcagccc tgcgtgagct acaataacag
     2401 ctaccagcat tacaaccgca ttgtcaaaga actggaaaca attccgggga aaccggacca
     2461 agccttaaaa ctggcaacgg acccggactc ggtcttcctg ggtgttttct atcaggagca
     2521 taagccggtg tttcacgaag agctgtatca aaaccgtaac cccttcagag acaggattcc
     2581 agcttcccat aggaccctgc cgtgaagaaa cctgtccagt tcaggcgaca tgtctggcag
     2641 attgtgtttc tgggggcgtc cttaacctgg ctggcggcca ccctcttcac acaacgcgta
     2701 aatatccacc aggcttgccc ttacgcggta gtgtgttttg gtgccatgga cgctgggatc
     2761 atcaatacgg ccagcaccgt tgccgtctgg gctgtgatcg tgggtttgct gatcgtgctt
     2821 tctacgatgt ggttgggccg tcgattctgt gcctggatat gcccgctggg aacggtgcag
     2881 gaaggcttgt tctcactaag gtcaaaatct tataaaatca aacgtaagtt gcctttttgg
     2941 tacgaccgta agttcgcgtg gatcaagtat tttctgctag gcctgaccac tttactggcg
     3001 gccctgactt tgagctggcg ctatatgcgc ctgtgtccct ttgtgactct tgcgcgttta
     3061 ccaggagtgg tgtggcaggg agctatcgta ttgttaataa tactggtaag ctcctttctg
     3121 gtggaaaggt ggtggtgcag gttcctttgc ccctacgcgg ccctgatgaa tttagcacag
     3181 aaactgggcc agttcatggg aatcaaccgc tcaaagatga aacgcaatct ggagcgctgt
     3241 aacgactgcg gagtctgcgt gttatactgt cccatgaatc tgaatctggc agagtccgaa
     3301 tatgtgcaat ccgagaattg catccactgc ggcttgtgcg cggaagtgtg tcccaaacct
     3361 ggtacttaca gcgaggagag tgaatgaaac aggccttcag aatcatgttt atagtgttgt
     3421 tactgagtgt gattagcctc cagatcgtca ctctgctgtc ttcccggcaa ggaacaagga
     3481 ctgtgcagat atgcccaacc ggagctatca gtatgcaggg ttccaaagcc gtgatcaaca
     3541 gcgaaaaatg catcggttgc cagcgttgtg tgcttggtat tccggtcaga tttccccagg
     3601 tccgtgacca agtcccccaa gtatcggttt cgcttccgca acgcgattcc atctcatccg
     3661 ctccgcttcc agctcctcca cccaaacagg cagatgctgt caaacccaaa gatgctgcca
     3721 ggtcaaatca accccaggcg atcaagccct ctgaagataa agagacctac caggtgaatt
     3781 ctgctgcatg tattggatgc cagctctgcg tgtcggcctg tcccacccag gcgatcagca
     3841 tggtaaacgg caaggcggtt atcgataagg aaaagtgtat caattgcggc atctgcgtca
     3901 atggcgacaa caacgagttt cagggatgtc cggtgggagc gatcaaggcc ccctgatcat
     3961 cttagggtat ccgctctctt acaggaaatt gctattgacc aaaagcatgg ctttgatatt
     4021 gtagcttttt aaggacttca cacgtctgtt tatgtttatt taataagcag atatgtcatg
     4081 atatgaacaa tcacaataat atagaatatg gaggaaccca tgagaaagac cgtggtggtg
     4141 agtgccaaac gtaccgcgat cggtaatttc ggcggtgtgt tcaaagacat ttccgcagtc
     4201 aaattgggag tgaacgtcgc caaggcgatt ttccaggaaa ccggcgtcaa accggaactg
     4261 attgaagaag tcatagttgg caacgtttgt ggagccggct atggccaaaa tccagcccgg
     4321 cagattgcca ttcacagtgg aattccggaa cacgtacctg cctatacagt taacaaggta
     4381 tgtggctctg gcatgaaagc ggtggccctg gccgccatga tgattggggc cggagaagcc
     4441 gatatcattc tggccggcgg cgctgaaaac atgagccaga tcccctacgc ctccatggat
     4501 gcccgttggg gtggtcgcat gggtaacaaa gaactggttg acctcatgat cagagacggc
     4561 ttgagcgaca tattcaacga ctaccacatg ggcatcactg ctgagaatct ggctgccaag
     4621 tatcagatca gcagagaaga gcaggacgct ttcgcggcct ccagtcagaa caaaactgag
     4681 accgcaatga acaccggaag attccaggac gagatcgttc ccatcgtgat ccctcagaaa
     4741 aaaggagacc cgctggtgat cgacagggat gaaatgccca ggccgggagt gacggctgcc
     4801 agtcttggca agctgcgtcc cgcttttaaa gctgacggca gtgtaactgc agccaattct
     4861 tccgggatca atgacggtgc cgctttgctg ttgttgatga gcgatgaaaa agccagagaa
     4921 ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaacggtgg agctatcgcg
     5041 ctgggtcatc cgattggggc tagcggcgcg aggataatcg tgaccctctt gcacgaaatg
     5101 aagaagcgcc aagtccataa gggtctggcg tctttatgca taggcggcgg aatgggaatc
     5161 gccacccttt gggaaatgtg aggtaatgat ggcagacaaa ctgaaactcg ataagagcat
     5221 ggcacttttt gccgaagctc aaactctcgt ccctggtggt gtggccggca tccgcaggcc
     5281 ctacaacttt gtggtgggag aatatcccat cttttttgaa gagggcaaag gcggtagaat
     5341 cactgatgtg gacggcaacg aatacatcga ttacctctgc gcctatggac ctatcagcat
     5401 cggttatcgg gaagacgaga tcgatgacgc ggtgatcgag cagatcaaaa acaagggatt
     5461 ctgtttctct ctgacccagg cgattcaaaa caagctggtc cgcaaacttc gtgaagtgat
     5521 cccttgctgc gaaatggccg cgatcgtcaa gaccggctcg gatgccacca ctatcgccat
     5581 cagaaccgcc agggcctata caggcagagt gaaaatcgcc cgctgcggat atcacggctg
     5641 gcatgactgg tgtgtggaag tgaaaggcgg gatcccggaa aaactatatg aagacattct
     5701 cgaattccat tataacgacc tggaccaggt ggaatccatc tgtaagcaac acggcgacga
     5761 tctggcagcg atcattgtca ctcccgttgg ccatcctctg gctgcggaag tggaaatgcc
     5821 caaacccggt tatctggaag ggcttagagc tctggccgac aagtatggct gcgtgctgat
     5881 ctttgatgag attcgcagcg gttttcgggt gtctttagga ggcgctcagg cctactttgg
     5941 cgtcacgcca gacctcgcaa ctttcggcaa agctatggcc aacgggtatg ccattgccgc
     6001 tttggtgggg aagagcgaga tcatgaacgt gatggcagac aaagtgttcc tgtcttccac
     6061 cttcttccca aacagcgacg ctcaggtagc cgcgctgaaa accattgaga tcctggagcg
     6121 cgacaaggtc accgatgtca ttgctgccaa aggatataag ttcgctgcag aagtagaggc
     6181 ggtggtaaag gaatctggca ttccggcccg ttttagcgga gctccctgga tgccttttat
     6241 cactttctcc aaggaccaga acggtctgta taagaaactc aggaacgagt tctataccca
     6301 gttgatcagg cggggggtat ttttgcagcc ttaccaccat ggctatatct gctatcgcca
     6361 cacagatgaa gatctggctt atacggtggg agcgatcaag gcctcgctgg cagatttgaa
     6421 gtcactctgt taacaggttg aagaagtggc aaaatatatc ttcgtgatgg gcggtgtcct
     6481 ttcctcgctg ggaaagggca tcgccaccgc cacaataggt ctgttgctca aagagatggg
     6541 ctaccgggtc tcgctgcaga agtttgaccc ctatctgaat gtcgatcccg gcaccatgag
     6601 tccctttcaa cacggcgaag tgtttgtcac cgacgatggc gccgagacag atctggacct
     6661 tggtcactac gagcgttttg tggatgtcgc gcttaccaga gactcaagct gttctgccgg
     6721 tcaggtatat gagacggtgc tgctcaacga gcggaagggc gtctatcttg gcaagaccgt
     6781 tcaggtgatt ccgcacgtga gcaacgagat caagcggcgt atcctgaagc agaacgataa
     6841 cttcgacgta atcatcacag aaattggtgg aacggtggga gatatcgaga gcctgccatt
     6901 cctggaatcg gttcgtcagc tccgcttgga actggggact cgaaactgcc tctgtgtttt
     6961 actcacctat gtcccctata tcaagaccgc tggagagctc aagaccaaac catctcagca
     7021 cagcgcttac aagctgcgcg aaatcggtat ccagccagat atcctgctct gccgcagcga
     7081 gaagcagttt gatgatgaaa tctacagcaa gatcgctctc tttaccaatg tgccaaaatc
     7141 gaatgttttc aacgcgatcg acgtcaagtg cgtgtatgag gtaccgatcg tctatcagaa
     7201 ggctggctta cccaagatag tatgtgacca ctttgaactt gaatacaggg agccgaaact
     7261 ggattcatgg gagcgttttt tgaaggcttc gtattccagc tctcccaaac tcacgatcgc
     7321 ggtatgcggc aagtatgtga agcatcagga tgcctataaa agcgtggaag aggctttgct
     7381 gcatgccgct gcttatcatg gccacaaact ggcgatcaag tgggtggatt cagaacggga
     7441 attctgtaat gaagacctgg acaaagaact ggatggcgta caaggaatcc tggtgcctgg
     7501 cggatttggt gtgcgaggaa tcgaaggcaa aatctcgatt gcccgctacg ccaggatcaa
     7561 aaacatcccc tatttgggtc tctgcctggg cttgcatgtg gccgtgatcg agttttcacg
     7621 caacgtctgt ggccttaaga atgccgatag cactgaattc gacgaattga acgagtatcc
     7681 agtggtccat atgatggaag atcagcagta tcaggagctt gtgggcggga cgatgcgtct
     7741 gggtgcctat ccctgtaaac ttgagcctgg cagccttgtc cacaaagcct atgaggcgga
     7801 cctgatcttt gagcggcacc gtcaccgcta cgaagtaaac aaccaatacc gggatttgct
     7861 tggcagcaaa ggactcaaga tcacaggcag ctcgccagac aacctcctgg tagagatcat
     7921 ggaactggag accaacgatt ttcacctggg agttcagttc catccggagt tcaaatccag
     7981 accccagcgt ccccatccgc tcttcaggga tttcgaggct gccgcgatca aatatgccat
     8041 ccgcaagggc cactagcagt gaagctaata atcatgcgga caaaacataa ggctttacgg
     8101 tctgaaataa tgcccacggt acggcagccg cggattgatc tgcagactct gtcatacccc
     8161 aagatttgtc ttgacagaaa aagcccctgt aaaaaaagtg cgacaacatt acggattgat
     8221 tgaacagcgc tgggggcgta gttcagtttg gttagaacgt ctgcctgtca cgcagaaggc
     8281 c
//
LOCUS       DAOSFN010000117         8275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_7390_length_8275_cov_8.694647, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000117 DAOSFN010000000
VERSION     DAOSFN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7390_length_8275_cov_8.694647"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="PLO35_07615"
     CDS             complement(<1..645)
                     /locus_tag="PLO35_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH79575.1"
                     /translation="MKGKVIILEDDVVLAEQISKVLKRFDYSVMHATNSDTFFEELRS
                     FAPDVILLDVFLVGSRLNGIQVLKYLKENLDLNYKIIVISGEVTSSQITQIRDLGAYH
                     FIEKGSNFSANQLLLHIDNAITLKRQEEEHIGLQIEYISLKKQYTRRFPFIGESEAIR
                     KVREQLSKLADVDEDVFLIGETGTGKEVAANYFYINSQRFGKAFHTVNCSALTES"
     gene            complement(777..1970)
                     /locus_tag="PLO35_07620"
     CDS             complement(777..1970)
                     /locus_tag="PLO35_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOH79576.1"
                     /translation="MLNVVFYFQVHQPYRLRHLNVLDIGTGTNLFDDRLNAEVMRKVS
                     NKCYLPTNQLLLDLIKENEGRFRIAYSITGTAIEQFKLYSPETLDSFKALAETGCVEF
                     LGETYYHSLAALYDTNEFLDQVMMHRQLIIDEFGQQPRTFRNTELIYQDRISDLVYEI
                     EGFTTIITEGVDRILQWRSPLFAYKNYSKDINLLLKYYKLSDDIAFRFSNRDWPEYPL
                     TVDKFVNWIDHLTLGEKGDRNLFLNLFMDYETFGEHQWAESGIFDFMRHFPSEVLKRP
                     HLGFVTPAETQALANYQQESLSFPDAVSWADEQRDLSAWVENDMQKNAIETFYELLER
                     VKTKGDPQLLKTARMLSTSDHFYYMCTKYFQDGDVHKYFSPYDSPDQAYIYYVNALAD
                     LEERI"
     gene            complement(1984..3324)
                     /locus_tag="PLO35_07625"
     CDS             complement(1984..3324)
                     /locus_tag="PLO35_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH79577.1"
                     /translation="MKILMFTWEFPPLISGGLGMACYGMVKALLALGIKIDLVLPTRE
                     MVYFPLREEEDVDKMPAVFMDPVHHSEFLTKSFQDITERLEYIGITPTPETYFQLSDM
                     RHFISKVKSEYLINEHVSQEEHDIWDDMTTHLIGEEDLIKKVQEYTQRAERFARELDY
                     NLIHVHDWLTYPSGMLARKISGKPLISHIHATEFDRAGGPGDERIHKIEHAGMTYSDR
                     VIAVSQYTAQMIMSRYRIDTGKIRIVHNAFSIEENQVHTKKRIFKGPTVLFLGRITLQ
                     KGPDYYLDVAERVLKVHPEARFIMAGTGDMARKLLRRSAQLKLKNRFLFTGFLNRKQV
                     ESILRAVDIYVLPSVSEPFGISPLEAMAFGITSIISKQSGVAEVVNHAFKIDYWDVDL
                     MADTINHLIENPQICKKMGIDGMKEVNRIHWNEAAEKIRMIYSQVLSEFLKSNP"
     gene            complement(3321..5357)
                     /locus_tag="PLO35_07630"
     CDS             complement(3321..5357)
                     /locus_tag="PLO35_07630"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOH79578.1"
                     /translation="MNNYFMETHHHEWILTNRRGAYALGTGNLINQRKYHGLLVASDA
                     KFQRRHLVAGIEEKVEWRGEIIHLDSNNYSNCIYPEGFLYLVKPWLRPYPAFLYSALP
                     HQDDILILKELMMDETSNTTLVKYSNLGHHTLHFHLHPKLTMALHHELNQPGSLDFEE
                     FATSESNGEDGCAFQARRVNNGAEVFCHSLLGQVEPNRYVYYNVYYPWEVMVGYPGIG
                     DQISLYELRFTLKPGQSNHILFSDALVDDPRAMCSRIEKRYAQLPQPADWPSKPDTDH
                     TLISSLDYNDNILFKRDGYLKLLEFALRDFQANDDIVAGYPWYGAWGRDTMIVLNALL
                     HAPNCVDQVEKVLRKYSRHLSGGLIPNMLPESGRELNYDSIDATLWYIILLWKLGKRK
                     QNVGYWKEVIHLCEEILKSILNNYQYPFEIRKDGLISLKSEFAHATWMDVRLDGKAVT
                     PRDGAPVEINALWYNALCCYEAMCESYQQASGIPYHPVELLTEVKDLVLKSFEKFWTG
                     EYLADRLTGDTPVLEIRPNAILALSLPWTPLSKERMKAVFLKAEAELLTPYGIRTLSS
                     KDTRFRKKYYGSQRERDLAYHNGSVWAWLLGAFCGLGVKVWRDELDDQALADKLRGYI
                     ATFRTSFCKGHIASLAEIWDGDNPHFPKGAPAQAISVAALYNIESFIAKLEGPK"
     gene            complement(5516..6472)
                     /locus_tag="PLO35_07635"
     CDS             complement(5516..6472)
                     /locus_tag="PLO35_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79579.1"
                     /translation="MKKIVSVIYLAVLVMGLTANGFTWYSQADSRWKQDNLGQSLSSI
                     GKSGCVLSCLSMLLNAEASNPRVTPDQLNSWLQRNGGYVGDLMRWQIPGEMDGNGLGL
                     ELVSQIDHANDWNYLSSELDKGNKVIVKVSNRRSHWVLVVKRDGPANVASSYIVNDPG
                     TSQYVQRTLAHWGGFKAARSYSGNWLDEQAFQLNSEIFVEPVAGDEEFLYQLVEQPIP
                     ANVYVTLSNALDVPISGFFILGLFDAQDSFVRVVDYQYSSCGAKGSVDLLYEMADITP
                     LEKSAGDLRIIYSKYFSSVPSLNEAIPLPSPGLINYSSSHNN"
     gene            6708..6956
                     /locus_tag="PLO35_07640"
     CDS             6708..6956
                     /locus_tag="PLO35_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOH79580.1"
                     /translation="MELKRHVVTIVIDDIETAFHPVTELLHTYAPNILLRVGYPMRDK
                     NVSVIFLVMELSVEAMGAFSGKLGQIRCVKVKSLTLKI"
     gene            6972..>8275
                     /gene="hydG"
                     /locus_tag="PLO35_07645"
     CDS             6972..>8275
                     /gene="hydG"
                     /locus_tag="PLO35_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOH79581.1"
                     /translation="MKISTEGVKSFIPTAKINELIKSQGHAPESRIREIIAKSLDKNR
                     LEPEETAALLSVQDPELKQLILHGAKELKERIYGNRIVLFAPLYVGNECVNDCVYCGF
                     RISNKECHRATLSKEQLVSETKSLVETGHKRLIMVYGEHPKYNPEFIADTVQTVYDTK
                     YKKGEIRRVNINAAPLDIEGFRTVKSVGIGTYQIFQETYHEETYKKLHPSGPKSSFLW
                     RLDGLDRAMKAGIDDLGIGALMGLYDWKFEVMGLLYHTIHLEQTFGVGPHTISFPRIE
                     PAIGTEFTEHPPYQVSDEDFKHLVATIRLSVPYTGMILTAREPIAIRNEVLSYGVSQI
                     DAGSSIGVGDYSSKDEESRSKSQFVLGDTRSLDEVIFELAKGGYIPSFCTSCYRAGRT
                     GEHFMEFAIPGFVKRFCTPNALLTFAEYLYDFSSQKTLNAGL"
BASE COUNT         1953 a         2081 c         2186 g         2055 t
ORIGIN      
        1 tgattcggtg agagcgctgc agttgacggt gtgaaaagcc tttccaaagc gttgggaatt
       61 aatatagaag tagttggccg ccacttcttt accagtaccg gtctctccaa tcaggaaaac
      121 atcctcgtcc acatcggcca gcttggaaag ctgctctctc actttgcgga tggcctcgct
      181 ttctccgata aaggggaagc ggcgggtgta ttgttttttg aggctgatat actcgatctg
      241 gaggccgata tgctcttctt cctgtcgttt gagcgtgatg gcgttgtcga tgtggagcaa
      301 cagctggttg gcactgaagt tagaaccttt ttctatgaag tggtaggctc ccaaatctcg
      361 aatctgggtg atctgggatg aggttacttc tcctgagatc acgatgatct tgtagttgag
      421 gtccagattc tctttgagat acttcaacac ctggataccg ttcaggcggc tccccacgag
      481 gaatacgtcc aggagaatga cgtccggagc gaagctgcgg agttcttcaa agaaggtgtc
      541 gctgttggtg gcgtgcatta cggaataatc aaaacgcttt aatactttgg aaatctgttc
      601 cgcgaggacg acatcgtctt cgagaatgat cactttacct ttcatcggaa tcctcaaaaa
      661 aagtggggct aaactcgctc ctaagctgtg gtatcagggt gatatagctg cgcgcaaagt
      721 ggatacgaac cggtaatact tcaccggcta tcaaaattgc taagtctctc atgatctcag
      781 atgcgctctt caaggtcggc caaagcattg acatagtata tgtaagcctg gtctggagaa
      841 tcatagggtg agaagtattt gtgcacgtct ccatcctgga agtatttggt gcacatataa
      901 tagaaatgat cagaggtgct cagcatgcga gctgtcttca gcagttgggg gtcgcccttg
      961 gtctttactc tttccagcag ctcgtagaag gtctcgatgg cgtttttctg catgtcgttt
     1021 tccacccagg cggagagatc tcgctgttca tcagcccagg aaacggcatc cgggaagctg
     1081 agagattcct gctggtagtt tgccagggct tgagtctcgg cgggagtcac aaacccgagg
     1141 tgaggtctct tcagtacttc gctggggaag tgacgcatga agtcaaagat gccggattcg
     1201 gcccattggt gttcaccaaa ggtctcatag tccataaaga gattgagaaa gaggttgcgg
     1261 tcgcctttct cccccaaagt gagatgatcg atccaattga cgaatttatc cactgtgagc
     1321 ggatattctg gccagtcgcg gtttgaaaag cggaaggcga tatcatccga cagtttgtaa
     1381 tacttgagca gcaggttgat atctttggaa tagtttttat acgcaaagag tgggctgcgc
     1441 cactgcaaaa tgcggtccac gccctcagta atgatggtgg tgaatccttc gatctcatag
     1501 acgaggtctg agatcctgtc ctgatagatg agctcggtgt ttctgaaggt gcggggctgc
     1561 tggccaaact catcgattat gagctgacgg tgcatcatta cctggtccaa aaactcgttg
     1621 gtatcataca gcgccgcgag gctgtgatag taggtctcac ccaaaaattc cacgcagcct
     1681 gtttcggcaa gggctttgaa ggaatcgagc gtttcgggac tgtagagctt gaactgctct
     1741 atggcggtgc ccgtgatgga ataggcgatt cgaaagcggc cctcattttc tttgatcagg
     1801 tcaaggagca gctggttggt aggcagatag cacttattcg acactttccg cataacctcg
     1861 gcgttgaggc gatcgtcaaa caggttggtc ccggtgccga tatcgaggac gttcaggtgt
     1921 ctgaggcggt aaggctgatg gacctggaaa tagaaaacta cgtttaacat ctatgctcct
     1981 tgctcaggga ttggatttga ggaattcgga aagtacctgg gagtagatca tgcggatctt
     2041 ttcagcagct tcattccagt ggatgcggtt cacttccttc atcccgtcga tgcccatttt
     2101 tttgcaaatc tggggatttt cgatcaggtg gttgatggtg tccgccatca ggtccacatc
     2161 ccagtaatca attttaaagg cgtggttcac tacttcggcc acgccgcttt gcttggaaat
     2221 gatggaagtg atgccaaagg ccatggcctc cagcggggag attccaaagg gctccgaaac
     2281 gctgggcagc acatagatat caaccgcccg cagaatggat tccacctgct tgcggttcag
     2341 aaatccggta aacaggaagc ggtttttcag cttcagctga gccgagcgcc gcagcagttt
     2401 tctggccatg tctcccgtgc cggccatgat aaagcgcgct tcgggatgca ccttcaggac
     2461 ccgttccgcc acgtccagat aataatccgg ccctttttga agcgtgattc gtcccaggaa
     2521 aagaacggtc ggccccttga aaattcgctt cttggtatgc acctgatttt cttcgatact
     2581 gaaggcgttg tggactattc ttatcttgcc ggtgtcgatg cggtagcgag acatgatcat
     2641 ctgggcggta tattgcgaaa cggcgatcac gcggtcggaa taggtcatgc cggcatgctc
     2701 gattttgtgg atgcgttcgt ctccgggacc gcctgcccgg tcaaattctg tagcatggat
     2761 atgggagatc aatggtttgc cggaaatctt tcgcgctagc atgccagagg ggtaagtaag
     2821 ccagtcgtga acgtggatga ggttataatc cagctctctt gcaaagcgtt cagctctctg
     2881 agtatattcc tgcaccttct tgatcaggtc ttcctcaccg atcaggtgag tggtcatatc
     2941 gtcccagata tcgtgctctt cctgagagac atgctcgttg atcagatact cgctcttgac
     3001 tttggagata aagtggcgca tatcgctaag ctgaaaatag gtttcaggcg tgggggtgat
     3061 gccgatatac tccaagcgct cggtgatgtc ctggaagctc ttggtgagaa actcgctgtg
     3121 gtggacaggg tccatgaata ccgccggcat tttgtccacg tcttcttctt cgcgcagtgg
     3181 aaaatagacc atctcccgcg tgggtaacac caggtcgatc ttgattccca atgccagcaa
     3241 ggctttgacc attccatagc aggccatgcc aagccctcct gagatcaggg gggggaattc
     3301 ccaggtaaac atcaaaatct tcatttgggt ccctccagtt tggcgataaa ggactcgata
     3361 ttatagagag cggccacgct gatcgcctga gctggagcgc ctttggggaa atgagggttg
     3421 tctccgtccc aaatctccgc cagtgaggcg atatggcctt tgcaaaagct ggttctgaag
     3481 gtggcgatgt agcctctgag cttgtcagcg agggcttggt catcgagttc gtccctccag
     3541 actttcacgc ccagaccgca aaacgcgcca agcagccagg cccatacact tccgttgtgg
     3601 taagcaaggt cgcgctctcg ctgagagcca tagtatttct tgcgaaaacg ggtgtcttta
     3661 gagctcaggg tcctgattcc ataaggcgtg agcagttccg cttcggcttt gaggaaaacg
     3721 gccttcatgc gctctttcga aagtggggtc cagggcaaag acagtgccag aatggcattg
     3781 gggcggattt caaggacggg agtatcgcct gtcagcctat ctgccagata ttctcctgtc
     3841 cagaattttt caaaagactt tagcaccaga tctttgacct cagtaagcaa ttccaccgga
     3901 tgatatggaa ttccggaagc ctgctgatag gattcacaca tggcctcata gcagcacaaa
     3961 gcattatacc acagagcgtt gatctccaca ggagctccgt cacgcggggt aaccgctttg
     4021 ccatccagac gaacgtccat ccaggtggca tgggcaaatt cgctcttcag gctgatcagg
     4081 ccgtcttttc tgatctcaaa gggatactga tagttgttca ggatggattt gaggatttcc
     4141 tcgcagagat gaatcacttc tttccagtaa cccacgtttt gtttgcgttt acccagtttc
     4201 cacagcagaa ttatgtacca aagggtggcg tcgatggagt cgtaattgag ttcgcgtccc
     4261 gattccggca gcatattggg gatcagacca ccgctgaggt gacggctgta tttgcgcagc
     4321 accttttcca cctggtccac acagttggga gcgtgcagga gagcgttgag cacgatcatc
     4381 gtgtctctgc cccaggcacc gtaccagggg tagccggcca cgatatcgtc gttagcctga
     4441 aaatctctga gcgcgaattc cagcagcttg agatatccgt ctcttttaaa caggatgttg
     4501 tcgttgtagt ccagtgagga gatcagggtg tggtccgtat ccggcttcga gggccagtcc
     4561 gcgggttgag gaagctgagc atagcgtttt tctattctgg agcacatggc gcgggggtcg
     4621 tccaccagag cgtctgaaaa gagaatgtgg ttgctctgcc ccggcttgag agtgaaacgc
     4681 agctcataca ggctgatctg gtctccaatg cctggataac caaccatcac ttcccagggg
     4741 tagtatacat tgtaatagac atagcggttg ggctccacct gtcccagcag gctgtgacag
     4801 aatacttcgg caccgttatt gactctgcgg gcctggaaag cgcagccatc ctcaccgttg
     4861 ctctcagagg tggcaaactc ctcaaaatca aggctgccgg gttggtttaa ctcatgatgc
     4921 aaggccatag tgagcttggg gtgcagatga aaatggaggg tatggtgccc cagattgctg
     4981 tatttgacga gggtggtatt gctggtctcg tccatcatca gctctttgag gatgaggatg
     5041 tcatcctgat gggggagggc tgaatacaga aaagcgggat aaggacgcag ccagggtttt
     5101 accagataca aaaagccctc gggatagatg cagttgctgt agttgttgct atccaggtgg
     5161 atgatttccc cgcgccattc cactttttcc tcaatcccgg ccaccagatg cctgcgttga
     5221 aatttggcat cactggctac caggagaccg tggtacttgc gttggttgat cagattgccc
     5281 gtcccgagcg cgtaagcgcc tctgcggttg gtaaggatcc attcgtgatg atgggtctcc
     5341 atgaagtaat tattcattga accccctagt tcaatagctg cttttctaat gtgttgattt
     5401 ttttctattc ccgcaatctc gtcaagtctt ttttccctca cataaggctt ccaggcctac
     5461 tcgcttgtga tgctgtcaca tagaactccg agacaagtgc ttgagaaaaa gcaactcagt
     5521 tgttatgact ggacgaataa ttgatcagtc cggggctggg aagcgggata gcctcattca
     5581 aagacggcac cgaggaaaag tatttgctat agataattct gaggtcgccg gctgattttt
     5641 ccaggggagt gatatccgcc atttcataga gcagatcgac actgcctttg gccccgcagc
     5701 tgctgtactg gtagtccacc actctcacaa aactgtcctg tgcgtcaaaa agacccagga
     5761 taaagaaccc gctgatgggg acgtccagag cgttgctgag cgtcacgtaa acgtttgccg
     5821 ggatgggctg ctccaccagt tgatagagga attcctcatc tcccgccact ggctccacaa
     5881 atatctcgct gttgagctga aaagcctgtt cgtccagcca gttgcctgaa tatgaacggg
     5941 ctgccttaaa gcctccccag tgtgccaaag tgcgctggac atactgactg gtgccaggat
     6001 cgttgactat ataggatgag gccacgttgg cagggccgtc ccgcttgacc acgaggaccc
     6061 agtgactgcg ccggttgctg actttcacga tcactttgtt gcctttgtcc agttccgagc
     6121 tgaggtagtt ccagtcgttg gcatgatcga tctggctcac aagttccagg cccagtccgt
     6181 taccgtccat ttcaccggga atttgccagc gcatcaggtc tccgacgtag cctccattgc
     6241 gctggagcca ggaattgagc tgatctgggg tcacacgggg attggatgct tccgcgttca
     6301 acagcatgga caggcagctc aacacgcagc cactcttgcc aatggagctg aggctctggc
     6361 ccaggttgtc ctgtttccat ctggaatccg cctgagaata ccaggtaaaa ccgttcgccg
     6421 taaggcccat aacgagcacg gcaaggtaga tgacactgac gatttttttc ataagcttaa
     6481 accaaaagga tgtgattgca aataaacaaa ttaagctatg aagacaagct gcgcgaccgg
     6541 ggcatttgtg tcaagccgaa aaaatcactt gtcaagtttc gcgcctcaga aatacagtac
     6601 ccaaaactat agtacctgag atgagggacc cgctccggca tcgagggtgg aagataccgt
     6661 aatatccacc ttgccagagc ctcccggtct caaccaagga tttggaaatg gaactcaaac
     6721 gccacgtggt caccatcgtt atcgatgata tcgagaccgc ctttcatccc gtcacggaac
     6781 tgctccacac ctacgctccc aatattctcc tgcgggtggg ttatcccatg cgtgacaaga
     6841 atgtatccgt gatctttctg gtgatggaac ttagcgtgga ggccatgggc gcgttttccg
     6901 gaaagctggg ccaaattcgc tgtgtaaaag taaaaagcct cactctgaaa atctgaacat
     6961 tggagatata aatgaagatc agcaccgaag gcgtcaagtc tttcatccct accgcaaaaa
     7021 tcaacgaatt gatcaaatcc cagggtcacg ctccggaatc ccggattcgc gagattatcg
     7081 ccaagtccct ggacaaaaac cgcctcgaac ctgaagagac ggccgccttg ctatctgtcc
     7141 aggatcctga gctcaagcag cttattctgc acggtgccaa agagctcaag gagcgcatct
     7201 acggcaaccg cattgtgctc ttcgctcccc tctatgttgg caatgaatgc gtaaacgact
     7261 gtgtatattg cggtttccgc atctcaaaca aagagtgtca ccgggccacc ctgtctaaag
     7321 aacaactcgt aagtgagacc aaatccctgg tggaaacagg ccacaaacgt ctgatcatgg
     7381 tctatggcga gcatccaaaa tacaatccgg aatttattgc cgacaccgtc cagaccgttt
     7441 acgacaccaa gtataagaaa ggagaaatcc gcagggtcaa catcaatgcc gcgcccctgg
     7501 atatcgaagg ctttcgcacc gtcaaatccg taggcatcgg cacctatcag atcttccagg
     7561 aaacctatca tgaagagaca tacaaaaagc tgcatcccag tgggcccaag agcagcttcc
     7621 tctggagact ggatggtctg gaccgcgcta tgaaagcggg catcgacgat ctcggtatag
     7681 gagctctgat gggcctctac gactggaaat ttgaagtgat gggcctgctc tatcacacca
     7741 ttcatctgga acagactttt ggcgtgggac cccacacaat ctctttccct cgcatcgaac
     7801 cggccatcgg aaccgagttt accgagcacc caccctatca ggtctcagat gaggatttca
     7861 aacacctggt ggccaccatc agactcagcg ttccctacac cggcatgatc ctcactgccc
     7921 gggaacccat tgccatccgc aacgaggtcc tcagttatgg cgtatctcag atcgacgccg
     7981 gatccagcat tggagtggga gattattcct ccaaggacga ggaatcacgc agcaaaagcc
     8041 aattcgtgct gggtgacacc cgtagcctgg atgaagtgat ctttgaactc gccaaagggg
     8101 gctatattcc gtccttctgc accagttgtt atcgtgccgg caggacaggc gagcatttca
     8161 tggaatttgc catacccggt tttgtgaaac gcttctgtac tcccaacgcg ctgctcacct
     8221 tcgcggaata cctctacgat ttttcatccc aaaagaccct caatgccgga ttgga
//
LOCUS       DAOSFN010000118         7982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_7786_length_7982_cov_8.544468, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000118 DAOSFN010000000
VERSION     DAOSFN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7786_length_7982_cov_8.544468"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="PLO35_07650"
     CDS             <1..549
                     /locus_tag="PLO35_07650"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOH79582.1"
                     /translation="VRIVPGGTSARNGCYIAPGVTIMPPAFVNIGAWVDSGTLVDSHA
                     LVGSCAQIGKRVHLSAGAMIGGVLEPIGSRPVIIEDDVFVGGNTGVYEGVIVSSRAVL
                     ASGTVITASTPVFDLTRNIFLDPDPEGSYTIPEGAVVVPGSRRARSNPDFQIYCPIIV
                     KYRDASTDAAVMLEDDLRHLFD"
     gene            596..1657
                     /locus_tag="PLO35_07655"
     CDS             596..1657
                     /locus_tag="PLO35_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOH79583.1"
                     /translation="MAKAPANAVNLALGELGYPLPQSLREHAMELLASGTPVYTPNAG
                     IPELRESIASTYGGATNPGCVCVCNGAQEALFLSLFSIIETGDKVAVPDPDYSAYCSI
                     LSILGANIIRLPLKRQLKSIDWDRWRNLLKDGVKVILLSSPNNPCGYYLKETDTNALA
                     SICADFGITVAIDEIYKGLCFAGTPASLTGKVEKLFIINGLSKSHCMTGWRLGWVVAP
                     EPLVATLTKAKQYVSTCSGWLSQQLAVFALGKIGRNAADKVFLQLLNSRGAALTILGS
                     ACPSSCLHVPDATPYLMLRCDDNDLHVAEKLAAKGVIVAPGSAFGKLSAGMIRINYGV
                     EVGMLEKGLKVIVDELYPH"
     gene            1638..3230
                     /locus_tag="PLO35_07660"
     CDS             1638..3230
                     /locus_tag="PLO35_07660"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOH79584.1"
                     /translation="MNYILTDARIMTMAPGTGGFAQADSLLVENGRIKSIGSKAECLS
                     SASRRYETISLQHKVLLPAFCDTHTHFVELAKGRFQLKLKGLNSIAAIKEAAQSYRDT
                     NAKLPDWVLGDGWDINIIDAPQYLNRKLLDELFPDVPVALFSKDYHSKWCNSLALKHS
                     GLLDALNSPFGDDLVKRDEDGLATGLIYEAATEYLERFVSPPSDKELQKAIKQELAHI
                     FSLGLGGFHSMESGASAAALTNAMNAGANFRCVWHFPLADLDHMITQGVTSYREQKSF
                     VIGGVKIFADGALGSKTAAMLQAYPDEPCNFGVLRYTQDELDAIIVKAAKHNIACTIH
                     AIGDLAVRNVIQCFYKHRISSQGNLLLQRIEHLQAIATEDLVRLQKSGAYCAMQPVHL
                     ANDVPMIEQSWRKVKDRAYRFDDILRLGIPYGFGSDAPIETIDPFKGIYSAIARKPCN
                     DPSQPSWFPKQRIGVLDALRGYTLAAAAGSLSHVWRGSLEPGKVADIIAIEDWSGKSD
                     EFWLEQRCSFCMVDGHINYIEL"
     gene            3248..>3969
                     /locus_tag="PLO35_07665"
     CDS             3248..>3969
                     /locus_tag="PLO35_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HOH79585.1"
                     /translation="MQKYGFDLPSQPRKTFLHELEESWYLPLLAKRKGIPLTAIKRSN
                     FGQYDMAVNYLSSVLDEASAINKVAIYNIDHMAQIRFWGKDCPTVLDRALAGNMSQMK
                     VGSCKYTLLLNERGGVQDDMILMRCSDSEFIMVINAGHDITASIEADGVKTELIADID
                     RVMSQKHPDDECWAEDISEQLVKVDIQGPYSFKLIKEIYGEEVLKNRNQPEKNMNFFS
                     FNEFERDGHLYYLSRTGYTNRWG"
     assembly_gap    3970..4069
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4070..4417
                     /locus_tag="PLO35_07670"
     CDS             <4070..4417
                     /locus_tag="PLO35_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HOH79586.1"
                     /translation="SHRAVYLNSVRLGSVTSSINSPNLTHAKRLAIGSSRKNVIDPEG
                     SAAIGLAWLYRVPPMTAGMDEGNPVGPHLPVDLFKEDAAGNPIGQALKGYLTTEGITP
                     ATAPKALKNIEPL"
     gene            4521..4829
                     /gene="trxA"
                     /locus_tag="PLO35_07675"
     CDS             4521..4829
                     /gene="trxA"
                     /locus_tag="PLO35_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH79587.1"
                     /translation="MVLELNTENFAAAIASGICLVDFWADWCGPCRMLAPTIDEIAKE
                     NMDITVGKLNVDDHPELAGKYNVMSIPTLLFFKDGQLKDNSVGVVSKNVLQRKIDALK
                     "
     gene            4840..5748
                     /locus_tag="PLO35_07680"
     CDS             4840..5748
                     /locus_tag="PLO35_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH79588.1"
                     /translation="MSAKRSKALTRVQNTVEYVLMRGLLLLLRLLPWSVAKSICQGLF
                     VIIGYHLGIRRAVAETQISKVYPEWEPRRRRQVLKELYRNMGLSIASIYLMKEESMLS
                     SCQVINREHVDAAYSLGRGVILATAHLGNWEAARVFPTKNMPVSVIVRRQRNRLFDDF
                     NNRVRSRHGVGIIDMRRGLRDIIAEFQKNHMVAILMDQNAGKSGHVMDFLGFPASHWV
                     GVAKLSLRYKVPILPGFALLTPEGGTRFTFEPMIYHPDWEDDESLYPSLLKEVNQVIE
                     DYIHRYPSQWFWVHKRWKGTGAMERD"
     gene            6083..>7982
                     /locus_tag="PLO35_07685"
     CDS             6083..>7982
                     /locus_tag="PLO35_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH79589.1"
                     /translation="MKRTLSVIILVCVVALLTAATSLELRPAFPAIKTEANVSVIELA
                     GGQLYGEPGDPALPWLGTKILLPVGEEAVSISVKLAQPQIYTLDKPLKPIPAQYPFSQ
                     TDLPAPVGPNPAIYGSDTVWPTQPHNGVNTHFLAGHPINFSAVCPFEYYPARHELIFY
                     ASVTVTVESAPSAKSTAALELLKQNEFTTQSLKNAINNQDALPRYQTRTSGVEYLIII
                     DSAKYDQWLPLADFYSQRGLNVMMKPVQEILSQTAGQDSQDKIRNYIIQMYQTNSLRY
                     VLLAADTDMIPHRGFYVNFTGGQQTDADIPADMYYACLDGNWNNDGDANWGEQYEADL
                     APELALGRICYNNDTEIANQINKIMMYHVAPVEAETKTAFFTGEWLWDGPTWGGDYMD
                     EMIGGASTHGYTTVGVPTSWNISTLYDRTYGYSEAWGPSEIRPMLSQGANLVNHLGHS
                     NTTYAMRLSNNQVTATSISNNGANHNFSLYFTQGCYAGAFDNRNTNPGSYTTDCIAEK
                     FTGIATSAASMIAHSRYGWGMQGSTDGASQYLHRQYIDAIFGENIHDLGFTLVDSKID
                     NIPFITNQPVMYWVTYETNLIGDPALSVWSDTPQQIVAQMPPMWSMSILGYQIPTNAP
                     YANLRIKSG"
BASE COUNT         1997 a         2080 c         1955 g         1850 t
ORIGIN      
        1 gtgcggattg tccctggcgg aacttctgct cgcaacggct gttacatcgc tccgggagtc
       61 acgatcatgc ctcccgcttt tgtcaacatc ggagcttggg tggacagtgg aaccctggtg
      121 gattcccacg cgttggtagg tagctgcgcg cagattggca aacgggtaca tctctccgcg
      181 ggcgctatga ttggcggtgt tttggaaccg atcggatcac gccctgtcat catagaagat
      241 gacgtctttg tgggtggaaa tactggcgtc tatgaaggag tgatcgtctc cagccgggcg
      301 gtgttggctt caggaactgt gataaccgcg tccacacctg tcttcgatct gacgcggaat
      361 atctttcttg atcccgatcc tgagggttct tacacgattc cggaaggagc agtagtggtt
      421 cccggcagcc gaagagcgcg ctccaatcca gactttcaaa tttactgccc cattattgtc
      481 aaataccgcg acgccagcac tgacgccgcc gtcatgctgg aagatgatct ccggcacctc
      541 tttgactgaa gcatcccgct ggcaaggggt ggaaatctcg ctgatgcgaa gcgtgatggc
      601 gaaagcacct gccaatgctg tcaatctagc tttgggagag ctaggatacc cattaccaca
      661 aagcctgcgt gaacacgcca tggaactgct ggccagcggt acgcctgttt atactcccaa
      721 cgccggcatt ccggagctaa gagaatccat agcatccact tatggcggag caaccaatcc
      781 cggctgcgtc tgcgtttgca atggcgcgca ggaagccttg ttcctcagcc ttttcagcat
      841 tatcgaaaca ggcgataaag tagcggtccc ggatccggac tacagcgctt actgcagcat
      901 tctcagcatc ttgggggcaa acattattcg cttaccttta aagcggcagt tgaaatccat
      961 agattgggac aggtggagaa acctgctaaa agatggagtc aaagtcatcc tgctcagttc
     1021 acccaacaat ccctgcggat attatcttaa agagacggat actaacgctt tagcctctat
     1081 ctgcgcggat ttcggcatca ctgtcgcgat agatgaaatc tataagggtc tttgttttgc
     1141 gggcacaccc gcatccttga caggtaaggt ggagaagctc tttatcataa acgggctctc
     1201 caaatcgcac tgcatgacgg gctggaggct gggttgggtt gtggcacctg agcctctggt
     1261 agccacgctc accaaggcca agcagtacgt gtccacctgt agtggttggc tgtctcaaca
     1321 gcttgccgtc tttgcacttg gcaaaattgg aagaaatgcc gctgataaag tatttttaca
     1381 gcttttaaac agccgcgggg cggccttaac gatcctcggc agcgcctgcc catccagctg
     1441 tttacacgtg ccagacgcca caccgtatct gatgttgcgc tgcgatgata atgacttgca
     1501 tgtcgctgag aaactcgcgg ccaagggagt gattgtggca ccaggttctg catttggaaa
     1561 gctaagcgct gggatgatca ggattaacta tggagtggaa gtgggaatgc tggaaaaagg
     1621 attgaaagta atagtagatg aattatatcc tcactgacgc cagaattatg acaatggctc
     1681 ctggaaccgg aggcttcgcg caagctgaca gcctgctggt ggaaaacggt aggatcaaat
     1741 ccatcggcag caaggctgag tgcctctcct ccgccagccg tcgctacgag actatctctc
     1801 tgcagcataa agtcttgtta ccggcttttt gtgataccca cacccatttt gtggagctcg
     1861 ccaaaggccg gtttcagctc aaactgaaag gcttaaatag cattgccgcg attaaggaag
     1921 cagctcagag ctatagagat actaatgcga aactgccaga ttgggtattg ggagacggat
     1981 gggatataaa tatcatcgat gcaccgcagt atttgaacag gaagctcctc gacgagctgt
     2041 ttcctgacgt ccccgtcgcg ctcttcagta aggattatca ttccaaatgg tgcaacagcc
     2101 tcgcgctcaa acacagcggt ttgctggacg cgctgaactc tccctttggc gatgatttgg
     2161 tcaagcggga tgaagatggt ctcgccaccg gtttgatcta cgaagctgcc accgagtatc
     2221 tggaacgatt tgtgagtccg ccctccgata aagaactgca aaaagccatt aaacaagaac
     2281 ttgctcacat tttcagcctc ggattgggag gttttcactc tatggaaagt ggagccagtg
     2341 cagctgctct gacaaatgca atgaatgccg gcgcaaattt tcgctgtgta tggcatttcc
     2401 cccttgcaga cctggatcat atgataactc agggagtgac ttcctaccgg gagcaaaagt
     2461 cttttgtaat aggtggggtt aagatatttg cggacggtgc cttgggctcc aaaacagccg
     2521 ctatgctgca agcgtatccg gatgagccat gcaatttcgg cgtcctgcgt tatacccaag
     2581 atgaactcga tgccataatc gtcaaggcgg caaagcataa tatcgcctgc actatacatg
     2641 ccattggtga tttggcagtc cgcaatgtta tccaatgctt ttacaagcat aggatttcca
     2701 gccagggtaa tctgctgtta caacgcattg agcatctaca agcaattgct acagaagatc
     2761 ttgttcgcct ccagaaaagc ggtgcctatt gcgccatgca accggttcac ttggccaacg
     2821 acgtccccat gatcgaacaa tcctggcgca aagtcaaaga ccgagcctac cgctttgatg
     2881 acatattgag gctcggtatt ccctacggtt ttggctctga cgctcccatc gaaacaattg
     2941 atcccttcaa gggcatctat agcgctatcg caagaaaacc ctgcaacgat cccagccagc
     3001 catcctggtt cccaaagcag cggattggcg tgttggacgc gctacgcggt tacaccctgg
     3061 ccgcggccgc tggttcgcta tcacacgttt ggaggggctc tttggagcct ggcaaggttg
     3121 cggatattat cgctattgaa gactggagcg gcaaatccga cgagttttgg ctggagcaga
     3181 gatgctcttt ttgtatggta gacggacaca taaattacat tgaattataa tatacaagga
     3241 gttacaaatg caaaagtatg gcttcgacct tccctctcag ccacgcaaga cattcctgca
     3301 cgagctcgaa gaaagctggt atctgcctct gttggcaaag cgcaaaggca ttccactcac
     3361 tgccattaaa cgcagcaact tcggccaata tgacatggcc gtcaactact tgagttccgt
     3421 gctcgatgaa gcctccgcca ttaacaaagt ggccatctac aacatcgatc acatggccca
     3481 gatccgcttt tggggcaaag actgtcccac cgttttagac cgcgccctgg cagggaatat
     3541 gagtcagatg aaggtgggct cctgcaagta cactcttctg ctgaacgaga gaggcggcgt
     3601 gcaggacgat atgatcctga tgcgctgttc agacagcgag tttattatgg tgatcaacgc
     3661 tggacacgat atcaccgcta gtatagaagc agatggtgtt aagacggagc tgattgctga
     3721 tattgatcgc gttatgtcgc agaaacaccc tgatgatgag tgttgggcag aagatatttc
     3781 agagcaattg gtgaaggtgg atatccaggg accctactca tttaagctca tcaaagagat
     3841 ctatggcgaa gaagtgctaa aaaaccgtaa tcaaccggag aagaacatga acttctttag
     3901 ttttaacgag tttgagcgtg atggtcacct gtattacctc agccgcaccg gctacaccaa
     3961 tcgttggggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ctcatcgggc
     4081 agtgtatctc aactcggtaa gactcggaag cgtgacctcc agcattaact cacccaacct
     4141 gactcatgcc aagcgtttag caatcggttc cagtcgcaag aacgtcatcg atccggaagg
     4201 ttccgcagcc ataggacttg cctggcttta cagagtacct cccatgaccg ctggcatgga
     4261 tgaaggcaac cccgtaggcc ctcatctgcc tgtagatcta ttcaaggaag acgccgccgg
     4321 aaatcccatt ggccaggcac tgaagggata tcttaccacg gaagggatta cccctgctac
     4381 ggctcccaaa gcacttaaga atatcgaacc gctttgatct aagtaactgc caagctgcaa
     4441 tatctgggga agcagagatt gagcttgtct ctgcttccct tatcgcgtat tatgtctcta
     4501 tcaatactta ggagagatca atggttttgg aactcaatac cgaaaacttt gccgccgcga
     4561 tcgcttcagg catctgcctg gtggattttt gggcagactg gtgtggtcct tgccgcatgc
     4621 ttgctcccac tatagacgag atcgcgaagg aaaatatgga tataacggtg ggcaaactga
     4681 atgtggatga tcatcccgaa ttagctggga aatataatgt tatgagcata ccaactctgc
     4741 tgttcttcaa agacggccag ctcaaagaca actccgtggg tgtagtaagc aaaaacgtgc
     4801 tgcagagaaa gatagacgcc ctgaagtaag aataccgcct tgtcagccaa acgctccaaa
     4861 gctctcacac gcgttcaaaa cacagtagaa tatgtcttga tgcgtggttt gctgcttttg
     4921 ctgcgcctcc taccctggag cgtggctaaa agcatctgcc aggggctctt cgtgatcata
     4981 ggttatcacc ttgggatacg gcgcgcagtt gctgaaacac aaatatctaa agtctatcca
     5041 gagtgggaac ccaggcgccg ccgacaggta ttaaaagagc tttaccgcaa catgggcctc
     5101 agcatcgcct cgatctacct gatgaaagag gaatccatgc tctcttcctg ccaagtgata
     5161 aaccgagagc acgtggacgc cgcctattct cttggccggg gagtgatcct cgccactgcc
     5221 catctgggaa actgggaggc agccagagtc tttcccacca aaaacatgcc cgtttcagta
     5281 attgtccgcc gtcagcgaaa ccgtctcttt gatgatttca acaaccgggt caggagtcgc
     5341 cacggagtgg gaatcattga catgcgtcgt ggcctcagag acatcatcgc agagtttcaa
     5401 aaaaaccaca tggtcgcaat cctgatggac caaaatgctg gtaaaagcgg tcacgtaatg
     5461 gacttcctgg gttttccagc ttcccattgg gtgggggtgg caaaactctc tttgcgttac
     5521 aaagtgccca tcctgccagg cttcgcgctt ctcacacctg aggggggcac ccgtttcacc
     5581 tttgagccca tgatctatca ccccgactgg gaagacgacg aatctctcta cccctcactc
     5641 ctcaaagaag taaaccaggt gatagaagat tacatccatc gttatccctc gcagtggttt
     5701 tgggtgcata aacgctggaa gggcaccgga gcgatggaga gagattgagg gttttaatgt
     5761 attggggttt cagggattaa ctgcagagat acagaggttt tctttctcac cacagaggac
     5821 acaaagggca cagagaaagg cacagagaaa ggtttttagg tgttatgtaa aaggtatttt
     5881 ttttaaaagg tttagatcac ctgccttctc aagctttcaa ccatatcaaa atctgcgtaa
     5941 tctgcgtgta aatctgcgta atctgcggga aacatactcc actccagcac tcccggaaat
     6001 gcctgcccca gaatcatcca aatgtggaac agagcttgca ttaattattg taaatatcta
     6061 atacatttaa agtaggtgct caatgaagcg aactcttagt gtgattattc tggtgtgtgt
     6121 cgttgccctc ctgaccgcag cgaccagtct ggaactccgt cccgcgttcc cagcaataaa
     6181 gacggaagcc aatgtcagcg ttatagaact tgctggcggt cagctatatg gcgagcctgg
     6241 cgatcccgcg ttgccatggc ttggcaccaa aattctgctt ccagtgggcg aagaagccgt
     6301 ctcaatcagc gtcaagctgg ctcaacctca gatttatacg cttgacaagc ccctgaagcc
     6361 catccccgct caatacccct tttcgcaaac ggatttgccg gcaccggtag gaccgaatcc
     6421 cgctatttat gggtccgata ctgtctggcc cacccagccg cacaacggtg tcaacacgca
     6481 tttcctcgcc ggacatccaa tcaacttcag tgccgtatgc ccctttgagt actaccccgc
     6541 acgccacgag ctgattttct acgcctcggt aacagtgacg gtggagagcg ctcccagcgc
     6601 caaatcaact gccgctctgg aacttctgaa acaaaacgaa tttaccactc agagccttaa
     6661 aaatgcgatc aacaaccagg acgccctgcc acgctatcag acccgcacca gcggagtgga
     6721 gtaccttatt atcatagaca gcgccaagta cgatcaatgg cttcccctgg cagattttta
     6781 ctcccaacgc ggcttgaacg tgatgatgaa gccggtgcag gaaattctct ctcaaacagc
     6841 gggacaggac agccaggaca agatccgcaa ctacatcatc cagatgtatc agaccaattc
     6901 cctgcgctat gttttactgg ctgcggacac agatatgatc ccccatcgtg gcttctatgt
     6961 gaactttacc ggcggccagc aaacggatgc cgatatcccc gctgatatgt attacgcctg
     7021 cctggacgga aattggaaca atgacggcga cgccaactgg ggagagcagt atgaagctga
     7081 cctcgctcca gagctggcgc ttgggagaat ctgctataac aacgataccg agatcgccaa
     7141 ccagatcaac aagatcatga tgtatcacgt tgcccccgtt gaagctgaaa ccaaaactgc
     7201 tttcttcaca ggtgaatggc tttgggacgg tcccacctgg ggtggagact atatggacga
     7261 gatgataggg ggagcttcca ctcatggtta taccaccgtg ggagtgccaa cctcctggaa
     7321 catcagcaca ttatacgatc gcacctacgg ctattctgaa gcctggggtc cctctgagat
     7381 tcggcccatg ttgtctcagg gcgccaatct cgtgaaccac ctcggccact ccaatactac
     7441 ttacgccatg aggctttcaa acaatcaagt gaccgccact tcgatctcca acaacggtgc
     7501 caaccacaat ttctccctct atttcaccca aggttgctac gccggcgctt ttgacaaccg
     7561 caacacaaac cccggcagct acaccaccga ttgtatagca gaaaaattca ccggcatcgc
     7621 cacctccgcg gcctctatga tagcccactc acgctacgga tggggcatgc agggttccac
     7681 ggatggggct tcccagtatc tgcaccgcca atatatcgac gccatttttg gcgaaaacat
     7741 tcacgatctg ggcttcacgc tggtggacag caagatagac aacattccct tcatcacaaa
     7801 ccagcctgtg atgtactggg tgacatatga aaccaatctg attggcgatc ctgccctcag
     7861 cgtttggtca gacactcctc agcagatcgt cgcccagatg ccaccaatgt ggagtatgtc
     7921 catcctcgga taccagatac ccaccaacgc tccttatgcc aatctcagga tcaaatctgg
     7981 aa
//
LOCUS       DAOSFN010000119         7930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_7865_length_7930_cov_9.725968, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000119 DAOSFN010000000
VERSION     DAOSFN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7865_length_7930_cov_9.725968"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="purM"
                     /locus_tag="PLO35_07690"
     CDS             complement(<1..977)
                     /gene="purM"
                     /locus_tag="PLO35_07690"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOH79590.1"
                     /translation="MNYEQSGVNVEAGEQAVRDIRSLVKRSYNSNVLSELGSFGGLFR
                     LDLAAHPNPVLVSSTDGVGTKLRVAIMAGVYDTIGQDLVNHCVNDILVQGAEPLFFLD
                     YIGMSSMDGLMVHDIISGMTSACQANGCALIGGEMAEMPGIYHDKDFDLVGTIVGVVD
                     HDHILPGDKVRERDLLIGIPSSGLHTNGYSLARAIVFDKLGLRVDSFVPELNATVAEL
                     LLSVHRSYLGSLKTFLTHPGLHGLAHITGGGIPGNLKRIIPAGLSAYVSYPMSEVPQM
                     FRWLQHAGGVSDAEMRKAFNLGIGMIAVVEENAAEELLNATGGIMIGEIV"
     gene            complement(974..1867)
                     /locus_tag="PLO35_07695"
     CDS             complement(974..1867)
                     /locus_tag="PLO35_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOH79591.1"
                     /translation="MSLVIVGSVALDSISTRSGSVSEALGGSAVYASIAASYFSEVRI
                     VGVVGEDYPQRGIDILKAHNVGLDGLETVPGKTFRWSGKYEDWNHADTLLTELNVFAG
                     FNPKLPPCCRNCRSLLLGNIHPSLQLQVLDQIDSYQWMACDTMNYWITGAPDLLEQVI
                     RRVDIVFMNEDEIRQFTRKDTIFSAARDILERGVKLVVVKRGEYGSVCIGKDHMYFAP
                     AYPLEKVVDPTGAGDCFAGGFMGYLATRDTLSPDVIREAVFRGTVLAAKDVSSFSVDG
                     IIQLDQSGINTMIDQLKVWTR"
     gene            complement(1864..2076)
                     /locus_tag="PLO35_07700"
     CDS             complement(1864..2076)
                     /locus_tag="PLO35_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79592.1"
                     /translation="MRDINERVRDRWEGRKAKNNNWVRLIVMVIILVALIVGINYLNT
                     AASRMSKAQAEFIDSTAVDSTGTSNP"
     gene            complement(2088..3290)
                     /locus_tag="PLO35_07705"
     CDS             complement(2088..3290)
                     /locus_tag="PLO35_07705"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOH79593.1"
                     /translation="MAIKLSNRTKLIKPSPTLTLSAKAKEMKDAGIDVINFGVGEPDF
                     PTPEYIKKAAHTALDDNFTRYTANAGIIELRKAICAKLSRDNGLSYEPKQILVSPGAK
                     ASILNILIAVCDSQDQVLMPAPYWVSYPYQAMLANAEAVIIPTSEAGHYKVTPATLKA
                     SCEANPCAKVLLLNSPNNPTGTVYTREELTELAKVCIEKNLLVISDEIYERLVYDGVE
                     HVSIAAVLPEMIDHTVVINGVSKAYAMTGWRLGYAAGPAHIIAAAGRVQEHATSCVNS
                     ITQKACVTALNEEDDSIEKMRAEFEQRRDFLCNELHKIPHISFFKPKGAFYVLPDISW
                     YLQNNDQGIKDCDQFCSILLEKYHVALVSGASFGIGCAVRFSYANSMENLQKGVQRFA
                     TYLSDLRS"
     gene            complement(3305..4306)
                     /gene="pta"
                     /locus_tag="PLO35_07710"
     CDS             complement(3305..4306)
                     /gene="pta"
                     /locus_tag="PLO35_07710"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOH79594.1"
                     /translation="MIDTLNRLKARAKEKGGTVVLPESADVRTLIGAADICGQGLAKV
                     ILVGDPVTVKERAHSTGVNIERDGIQIIDPNSYPRIDEFASWFYEKRRDKGLCQEMAA
                     SLVRNELYFGALLVKFGIADGMVAGAANTTGNVLKAALQVVGVASGLKTVSSSFIMVV
                     PDYFGETRLFSFADCAVVPNPTAEQLADIAISTAQTRRALLDDEPRVALLSFSTHGSA
                     KHELVDKVLEAREILKSRQVDFLFDGELQLDAAIVPKIAQSKAPSSPIEGHANVLIFP
                     DLQAGNIGYKLTQRLAKAEAIGPVIQGLAAPVCDLSRGCSAEDITNTAVLVLLMRKN"
     gene            complement(4307..6409)
                     /gene="glyS"
                     /locus_tag="PLO35_07715"
     CDS             complement(4307..6409)
                     /gene="glyS"
                     /locus_tag="PLO35_07715"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOH79595.1"
                     /translation="MNSREFLFEIGVEEIPAAHFGPACEWLRESFSNFIRDSRLSFET
                     LKVSGTPRRFFVLASGIPVSQPDIVLERTGPALRIAYTEDGQLSPAALGFLKKNSASP
                     SDVKVQKTDKGEFIAISIHQDGRPSADLLSEWACNLLAAFPFPKQMIWRSRDLSFSRP
                     VRWLCALWGEDVLPLDSHGIPCGRITYGNRYLGLDAPVSISSPSAYLDVLKGVSVVAD
                     REERLSILNQSLQASLASPDLRVLPDARLAETVCDLVEWPTAVAASFEERFLELPEKI
                     ITSTISQNQKYFSVSHACGKLSNHFVFISNGDPAKSDIIRHGNEKVVKARLADALWYF
                     EEDCRKPLESYVPALGEVVFQARLGTMAAKVKRVMALSQWITDKLSLEPEIREKVLRT
                     AMLAKADLVTNMLGEKEFTKLQGYIGKQYALACGEDPEVAEGIWEHYAPRGSNDSLPV
                     SLTGAIVAVADKVDTVCGIIGAGLMPTGSADPFALRRAANGVCQILMERGWELELEEL
                     ASQSFSILTASLDLEADAHDKAQIFFRQRAEWLLKQEGIAYDVIDSLAHLPIGSLAAF
                     HKQAMAIGRIKPTEDFTRLVIGFKRVANIVGDKPVQTAPDSSVFTEPEETALWEAHSS
                     LREALDKALFQRDFQAALIALVDMGRPIDSFFDAVLVNCEDEITRQNRHALLAEIKRQ
                     FIRVADISRIVIETENGA"
     gene            complement(6427..7383)
                     /locus_tag="PLO35_07720"
     CDS             complement(6427..7383)
                     /locus_tag="PLO35_07720"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOH79596.1"
                     /translation="MSTRSKIISITITLSLSLAGLWSVNENAGTTGFNALKMVHGARA
                     MAVGQSLTGEAINPDGLHFNPAAILNVPGPEVSTTYCNYFVDAQGGQVQLLLPKNKFT
                     AWGLALKYMNMGTIERTEVDQFDNLIDDLGTFGAYNVTASVSLAKFVSKALDFGGSLK
                     VIYDTLDDASAAAVLVDLGIIHHPFNEKVKVGVSLRNMGAQLSYYSDSKYKEKLPFTF
                     AAGGSYRFSDKNYSLVEINKADGENFNLRLGHEYNLTPDFALRAGFRTNAADWNNGGA
                     MGWSSGLSLGAGLNWKNYRFDYAVSSYGDLGLVNQLSLVYEF"
     gene            complement(7340..7810)
                     /locus_tag="PLO35_07725"
     CDS             complement(7340..7810)
                     /locus_tag="PLO35_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOH79597.1"
                     /translation="MNRLFQPELVRIVDRFDTKGQCLEYLTQLMADSGCLSFPDRFLA
                     AVKGREEIMSTGIGKGVAIPHARDLTVSCLRISVCMLREGLDFQAVDAQPVRLVFMIA
                     VPQSSNKEYMKILRSLSEYLRQDANRNLLNSATSERELFDYVHQIQDHIDHDNA"
BASE COUNT         1671 a         2161 c         2283 g         1815 t
ORIGIN      
        1 acgatctctc cgatcatgat cccgccggtg gcattgagca gctcttccgc ggcgttttct
       61 tccaccacgg cgatcattcc gatccccagg ttgaaggcct tgcgcatctc ggcatcggat
      121 actccgccag cgtgttggag ccatctaaac atctgcggca cctcgctcat gggatagctc
      181 acataagcgc tcaacccagc ggggatgatc cttttgaggt tgccgggaat tccacccccg
      241 gtgatgtggg ccaggccatg caggccagga tgagtgagga aggttttcag gctgcctaaa
      301 tagctgcgat ggacgctgag cagaagctcc gcgaccgtgg cattgagttc cggtacaaag
      361 ctgtccaccc tgaggcccag tttatcgaag acgatggcgc gggccaaaga atagccattg
      421 gtatgtaaac cactggaagg gataccgatc agcaggtcgc gttccctcac tttatcgccg
      481 gggaggatgt ggtcatggtc caccacgcct acgatggtgc ccaccagatc aaagtctttg
      541 tcatgatata tgccaggcat ttcggccatt tcgccaccga tcagggcaca gccgttggcc
      601 tggcaggcgc tggtcatgcc gctgatgatg tcgtgcacca tcaggccgtc catgctgctc
      661 atgccaatgt aatcaaggaa aaagaggggt tcggccccct ggacgaggat gtcgttgacg
      721 cagtggttga cgaggtcctg tccgattgtg tcatagactc cggccataat ggcgacgcgg
      781 agtttggtgc ccacaccgtc ggtactggac accagcacgg ggttgggatg agcggccaga
      841 tcgagtctga agagcccgcc aaagctaccc agttcactga gcacgttgga gttgtagcta
      901 cgcttcacca gcgagcggat gtcacgaacg gcctgctcgc ccgcttccac gttgacgcca
      961 gattgttcgt aattcatctt gtccacacct taagctgatc tatcatggtg ttgatgccgc
     1021 tctggtccag ttggattatg ccatcgacgc tgaagctgga gacgtctttg gcggccagga
     1081 cggtgcccct gaacacggct tccctgatga catcgggtga gagggtgtcc cgggtagcca
     1141 gatatcccat gaagcctccc gcgaagcaat ctcccgctcc ggtgggatcc acaaccttct
     1201 ccagcggata ggccggagcg aaatacatgt gatctttgcc gatacacacg ctgccatact
     1261 ctccgcgctt caccaccacg agcttcacgc cccgttccag gatatcccgg gcggcactga
     1321 agatggtatc ttttctggta aattggcgga tctcgtcttc gttcataaac acgatatcca
     1381 ctctgcggat cacctgttcc agcaagtctg gagcgccggt gatccagtag ttcatggtgt
     1441 cacaggccat ccactgatag ctgtcgatct ggtccagcac ctgaagctga aggctgggat
     1501 ggatgtttcc cagcagcaga ctgcgacagt ttcggcagca gggaggcagc ttgggattga
     1561 atcccgcgaa cacgttcagc tctgtgagca gggtgtcggc atggttccag tcttcatact
     1621 taccgctcca gcggaaggtt ttgccgggaa cggtctcgag tccgtccaac cccacattgt
     1681 gggctttcag gatatcgatg ccgcgctggg gataatcctc acccaccact cccacgatgc
     1741 ggacctcgct aaaataggag gcggcgatgg aagcatagac ggcagaaccg cccaaggctt
     1801 ctgaaacgct gccggagcgg gtgctgatgc tgtccagggc tacggagccc acgattacaa
     1861 ggctcatgga ttgctggtcc cggtgctgtc cacagcggtg gaatcgataa actcggcctg
     1921 agctttgctc atccggctgg cggcagtgtt taaatagttg attcccacta tcagggcgac
     1981 caggatgatc accatgacga tgagccgcac ccagttgttg tttttggctt tgcggccttc
     2041 ccagcgatca cgcacgcgtt cattaatatc tctcatcgca tgcctcttca gcttctcagg
     2101 tcagaaaggt aagtggcaaa gcgctgcaca cccttctgga ggttttccat gctgttggca
     2161 taggagaatc tgaccgcgca gccgattccg aaggaagctc ctgaaaccag ggcaacatgg
     2221 tatttttcca gcaggatgga gcagaactgg tcgcagtcct tgatgccctg atcgttgttt
     2281 tgcaggtacc aggagatgtc tggcagcacg tagaaggcgc ctttgggttt gaaaaaagag
     2341 atgtggggga tcttgtgcag ctcgttgcag agaaaatcgc ggcgttgctc aaattcggcc
     2401 cgcatctttt cgatggaatc atcttcttcg ttgagagcgg tgacgcaggc tttctgggtg
     2461 atggagttca cacaggaggt ggcatgttcc tgcacccttc cggccgcggc gatgatatgg
     2521 gcaggaccgg cagcgtagcc aagcctccag cccgtcatgg cataggcttt ggagacgccg
     2581 ttgatcacca cggtgtggtc gatcatttca ggcagcacgg cagcgatgga aacgtgttcc
     2641 accccgtcat agaccaggcg ttcatagatc tcatccgaga ttaccagcaa gttcttttca
     2701 atgcatactt tagccagttc ggtgagctcc tcgcgggtgt acacagtccc ggtgggattg
     2761 ttgggtgagt tgagcagcag cactttggcg cagggattgg cttcacagga ggctttgagg
     2821 gtcgcggggg tgaccttgta atgtccggct tcgctggtgg gaatgatcac ggcttcggca
     2881 ttggccagca tggcctgata gggatagctc acccaatagg gagcgggcat caaaacctgg
     2941 tcctggctat cgcaaacggc gatcaggata ttgaggatag aagccttggc gcctggagag
     3001 accaatatct gtttgggctc ataactgagg ccgttatcgc gtgacagctt ggcgcagatg
     3061 gccttgcgga gctcaatgat gccggcgttg gcggtgtagc gggtgaaatt gtcatccaga
     3121 gcggtatgag cggccttttt gatgtattcc ggagtgggaa agtccggctc tccgaccccg
     3181 aaattgatga cgtctatgcc ggcatccttc atctctttgg ctttggcgga aagagtgagg
     3241 gtgggcgacg gtttgatgag cttggtgcgg tttgaaagct tgatcgccat tttgggcctc
     3301 cttatcaatt tttgcgcatt aacaacacca gcacggcggt gttggtgatg tcttcagcag
     3361 agcagcctct ggaaagatcg cagactggag ctgccaatcc ctgaatgacc ggaccgatag
     3421 cttcggcctt ggccagtcgc tgcgtgagct tgtatcctat gttcccggcc tgtaagtccg
     3481 ggaaaatcaa gacgttggca tgcccctcga tgggactgga gggggctttg gactgcgcaa
     3541 tcttgggcac gattgccgcg tccagttgca gctctccgtc aaagaggaaa tcaacctggc
     3601 gggatttgag tatctctctg gcttccaata ccttgtccac cagttcatgt ttggcgctgc
     3661 cgtgggtgga aaaggagagc agggccaccc ggggttcgtc atccaacaga gcgcggcggg
     3721 tctgagctgt gctgatggct atgtctgcca gttgttcagc ggtggggttg ggcaccacgg
     3781 cgcaatccgc aaagctgaag aggcgggttt ccccaaagta atccggcacc accatgataa
     3841 agctgctgga gacggtcttg agcccggaag ccactcccac cacctgcaaa gcggctttga
     3901 gcacgttgcc ggtggtgttg gcggccccgg caaccatgcc gtcggcaatg ccaaacttga
     3961 ccaataaagc tccaaaatag agctcgttgc gcaccaggct ggctgccatt tcctggcaga
     4021 gtcctttatc ccggcgcttt tcataaaacc agctggcaaa ttcatcgatg cggggatagc
     4081 tgttgggatc gatgatctgg atgccatccc gctcgatatt cacacctgtg gagtgggccc
     4141 tttccttcac ggtcacgggg tcgcccacca ggatgacttt ggccagtccc tggccacaga
     4201 tatcggcagc gccgatcaga gttctcacgt ccgcgctttc aggcagtaca actgtcccgc
     4261 ctttttcttt ggcgcgggct ttgagtctgt ttaaagtgtc gatcatttat gctccgtttt
     4321 cagtttctat tacaatcctg gagatatccg ccaccctgat aaactggcgc ttgatttctg
     4381 ccaggagggc gtgacggttt tggcgcgtga tctcatcctc gcaattcacc agcaccgcgt
     4441 caaagaagct gtcgatcgga cggcccatgt cgaccagggc gatcagagcg gcctggaagt
     4501 ctctctggaa gagcgctttg tccagcgctt cccgcaatga gctgtgggct tcccacagcg
     4561 cggtttcctc aggctctgtg aagacagagg aatcaggggc agtctggaca ggcttgtctc
     4621 ccacaatgtt ggcgacccgt ttgaagccaa tcaccagccg ggtgaagtcc tcggtgggct
     4681 tgatcctgcc gatggccatg gcctgtttgt ggaaggcggc cagcgaaccg atgggcagat
     4741 gagcaaggct gtcgatgaca tcgtaggcga tgccttcctg cttgagcagc cattcagccc
     4801 tttggcggaa gaagatctgt gctttgtcgt gggcgtcggc ttccaggtcc aggctggcgg
     4861 tcaggatgct aaaactctgt gaggcgagct cttccagttc cagttcccag cccctttcca
     4921 tgaggatctg gcacacacca ttggcggctc tgcgcagggc gaaaggatcg gctgagccgg
     4981 tgggcatcag acccgctcca ataataccgc agactgtgtc cactttgtca gcgaccgcaa
     5041 ctatagctcc ggtgaggctg actggcaggc tgtcgttgga accacgcggc gcgtagtgtt
     5101 cccagatgcc ttccgccact tcaggatctt ctccgcaggc cagagcgtat tgcttgccaa
     5161 tatatccctg cagcttggta aattcctttt cgcccagcat gttggtcacg aggtcggcct
     5221 tggccagcat agcggtgcgc agcacttttt cccggatctc aggttccagg ctgagcttgt
     5281 cagttatcca ctgactgagc gccatgacgc gcttaacctt ggcggccatg gtgccaagcc
     5341 gggcttgaaa caccacttcc ccgagggcgg ggacgtagct ctcgagcggc ttgcggcaat
     5401 cttcctcaaa ataccagagg gcgtcggcga ggcgggcttt taccaccttt tcattgccgt
     5461 gacggatgat atcgcttttg gcaggatcgc catttgagat gaagacaaag tggttgctga
     5521 gctttccaca ggcatgggag acggaaaaat acttctgatt ctgactgatg gtggaagtga
     5581 tgatcttctc agggagttcc agaaagcgct cttcaaagct ggctgccact gccgttggcc
     5641 actccaccag gtcgcagacc gtttccgcca ggcgggcatc tggcagcact ctcagatctg
     5701 gtgaagcgag gctggcttgc aggctttgat tcaggatgga aagcctctct tcccggtccg
     5761 ccactacgga cacgcccttg agaacatcca gataagcgga tggcgaggat atgctgaccg
     5821 gagcatccag gcccagataa cgattgccgt aagtgatcct cccacaaggg atgccgtggc
     5881 tatccagagg caaaacatcc tctccccaca aggcgcaaag ccagcgcacg ggacgtgaaa
     5941 agctcaggtc acgggagcgc cagatcatct gtttgggaaa gggaaaggct gccagcagat
     6001 tacaggccca ctcgctgagc aggtccgcgc tgggacgtcc gtcctgatgg atgctgatag
     6061 cgataaactc ccccttatcg gtcttttgga cctttacatc ggaggggctg gcactattct
     6121 ttttgagaaa acccaaggcg gcggggctga gctggccgtc ttcggtataa gcgatcctga
     6181 gggcgggacc agtgcgttcc aacactatat ccggctgaga tacaggtatt cccgaagcga
     6241 ggacaaagaa gcggcgtgga gtcccgctca ctttgagcgt ttcaaagctg agccgggaat
     6301 cccggataaa attgctgaag ctctctctga gccattcaca ggcaggaccg aaatgagcgg
     6361 cgggaatctc ttccacgccg atctcgaaca aaaattcgcg gctattcaaa gcagtaccgt
     6421 gccggatcag aattcgtaaa cgaggctgag ttgatttacc aggcccaagt ccccatagga
     6481 ggatacggca tagtcgaacc ggtagttttt ccagttcaaa cccgcgccca gagacaatcc
     6541 agagctccaa cccatggcac cgccattgtt ccagtccgcg gcattggtcc tgaagccggc
     6601 cctcagcgca aaatccggcg tgaggttata ctcatgcccc agacggaggt tgaagttttc
     6661 accgtcggcc ttgttgatct ctacgaggct gtagttttta tcggaaaagc ggtaagaacc
     6721 acccgccgcg aaggtgaatg gcagcttttc tttatatttg ctgtcgctgt aatagctgag
     6781 ttgagcgccc atgttgcgga ggctgacccc caccttcacc ttctcgttga agggatgatg
     6841 gatgatgccc aaatccacca gcacggctgc cgcggaggca tcatccaggg tgtcgtagat
     6901 gactttgagg ctgccgccaa aatccagggc tttactgacg aacttggcca aagaaacact
     6961 cgcggtcaca ttatacgccc caaaagtgcc gaggtcgtcg atgaggttgt caaactggtc
     7021 aacctcggtg cgctcgatcg tgcccatatt catatattta agggccagtc cccaggcggt
     7081 gaacttgttt ttgggcaaga gcagctgtac ctggccgccc tgagcatcca cgaaataatt
     7141 gcagtaggtg gtgctgactt cgggaccggg gacgttgaga atggcggcgg gattgaagtg
     7201 cagcccatcc gggttaatgg cttctcccgt aaggctttga cccacggcca tagctcgcgc
     7261 gccatggacc atcttgagag cgttaaatcc ggtagtgccg gcgttttcat tgacgctcca
     7321 gagccctgcc aaagacaggc taagcgttat cgtgatcgat atgatcttgg atctggtgga
     7381 catagtcaaa aagctccctt tcgctggtcg cgctgtttaa aaggttgcgg ttggcatcct
     7441 gcctcaggta ttcggacagg ctgcgcagga ttttcatata ttccttgttg gagctctgcg
     7501 gcacggcgat cataaagacc agcctcaccg gttgagcatc cacagcttga aaatccaggc
     7561 cctcccggag catgcacaca ctgatgcgca gacaggagac ggtaaggtcc ctggcatggg
     7621 gaatggccac ccctttgccg ataccggtgc tcatgatctc ttcgcggcct ttcacagcgg
     7681 ccagaaagcg atcgggaaaa ctcaggcatc cgctatctgc cataagctgg gtcagatact
     7741 ccaggcactg acctttggtg tcgaatctat cgacgatccg gactaattcc ggttgaaaaa
     7801 gtctgttcat cttctcacca aacattagat tagaatacat cgttttctga aggcagatac
     7861 tcgtcaatca aaattgttag agccttcggc tggttagagc agttcctgct ggttagagca
     7921 gttcctgctg
//
LOCUS       DAOSFN010000120         7879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_7934_length_7879_cov_10.067101, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000120 DAOSFN010000000
VERSION     DAOSFN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7934_length_7879_cov_10.067101"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..2033
                     /locus_tag="PLO35_07730"
     CDS             126..2033
                     /locus_tag="PLO35_07730"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOH79598.1"
                     /translation="MSKHEQDTIATLQARSKAARAAILTMTTLSASGHPGGSMSAIDL
                     LNCLYHTIRHDPSQPLLESRDRVVVSNGHISPAVYAILGLQGYFPLDEAISQFRLAGS
                     RYEGHIEREVPGVEWSTGNLGQGLSAGVGMALAARVKQSEHRVYVMMGDGEQQKGQIG
                     EARRLAVKYQLSNLCAIIDYNRLQISGSIHEVMPQNIRRNWEADGWAVLEVNGHDFEQ
                     ILETLEQARRQDKPVMVLAHTVMGKGVDFMENLAKYHGSTLNPEQLDTALKQLGEPNR
                     LEALKALRQKTELPVSGNCDMSFALKADLQGGQPIVYEKDTDNRSAWGNAITNLAEIN
                     KSSPTPIVVLDCDLASSVKTNDFAARFPERFFQGGIMEHNTAVVAGAISSCGIQTFWA
                     EFGMFGLAEVYNMQRLNDINHSNLKVVLTHVGLDVGEDGKTHQSVDYISLVRNLHGFR
                     LILPADPNQTDRIIRWLINKPGNYIVAMGRSKLPILKTEDGEIFFKMDYNFEYGKADL
                     LRVGNHGTVLVTGTPSARALKAVDKLREQGIYPQLYYVSSPCDLERSLLDEIARAKRV
                     VTIEDHNVKGGLGSILADRLAEEGLCASLQKIGVRDYPVSGAADELYKRAGMDPSTLA
                     EKISAWIQGPR"
     gene            2333..4330
                     /locus_tag="PLO35_07735"
     CDS             2333..4330
                     /locus_tag="PLO35_07735"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH79599.1"
                     /translation="MKRLLVLYFALLISLSCQAGRKALVIGNAIYNVNLLSSPAKDAE
                     LVAETLTQLGFTVKHVADIDYNTFDAELKNFTGSLSGGDEAFFYFGGIALQLEGENYL
                     LPTGFNYKSANDIRFFSIPLKRVLEDMSKANYAMLFVDGSRENSFSTLAGGQIGLAEP
                     AAVPPNAILQFSTAPNVWLESDLLPTSKYAQSLVNQLRISAQNMEKICSATSETVARL
                     TAEAQIPWTVGSLPNYYYINNNIINDGGSGLDDSIVADNMTFKEMDLQPGIVTSTGLE
                     SSPAPVIYTGDDAHKLYKTSKFGNIIDVFSASEFIMTQSKDSNDLSQFASWNMVRLNL
                     AMFGAEAYYKKMLVWYKGAGTQGSGLGVKQTDITQMGAGAGLQNPDFIRLFATMNKYE
                     YSDPNVNPLWGEDLADYYNYSFSFAKRVRANRQSLQLGADFLYNPEGYPEAFLFQTSP
                     FPQAYSFDDWDFAHYPSWKLSSYLYTTDVYTDAHESLHLAGVAPKDRPLLFTRQDGVL
                     FGLTFNMEERSGQSYSMEDYQRYQFSVLLHKNFGKAVGFDFNFDYNDSLMLDTDEHYK
                     NNQFRGTMRLTLVDAGPLALSTAIDYVNRGQLSSGTDPDEATPERMQGINGAAFLILD
                     ISKRLIISGMGQINMNWFPSDTLFKLEQTEFFFGGTVGLRL"
     gene            4463..4849
                     /locus_tag="PLO35_07740"
     CDS             4463..4849
                     /locus_tag="PLO35_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH79600.1"
                     /translation="MKKFLKTMPDQCIACHVCESTCSNLYFKTDDAELSCIRITETED
                     LPHMNACNQCEACVKVCPTKALTVSPQGVVLVNKKLCIGCYMCIAACPTGSMFRSQGG
                     LYPFKCIACGACVKTCPADAIRVVQE"
     gene            4860..7004
                     /locus_tag="PLO35_07745"
     CDS             4860..7004
                     /locus_tag="PLO35_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOH79601.1"
                     /translation="MQKPLRKVLAEFNYELAPVIKGYNRRTLYINVGEKAIKSKPVTD
                     MMIDKFVGGKGFDLFLMWHGVQDDTHWDSPENEICISFGPLCGNTSYPGSGKSIVTTI
                     SPLTGIPVDCNVGGHFGPYSKFSGWDAIEIQGKADEEVIVYIDGDKGIVQILSAPDEE
                     INSHILAERLIEEFADDPEKHQFVSVVSSGKGAEHVPISCLNFSFWDKRRKVARLKQA
                     GRGGTGMVFRDKKIKALVVKYSGLKGDTNNPVDLETLQKTGLKLHKEIEAGDSTQCHM
                     REIGTAHLMEIMDDYDLLPIRNFKYGQMPEARALASWEFKNLFTQGMADGCWYGCSLS
                     CCKAVDNFVPRSGPYKGHKVCVDGPEYETAAGCGSNLCIWDPKDVVELNFYCDTYGID
                     TISFGTSVAFAMECFENGILNLERTGGIDLSWGNADSVFELIHQMARGEGFGVTVGQG
                     VRKMKDIFAREYGADPAFLQDIGMEAKGLEYSQYMSKESLAQQGGYTLALKGPQHDEA
                     WLIFMDMVNNHIPTFEDKAEALHYFPMFRTWFGLQGLCKLPWNDIEPANNAETDEPAK
                     VPEHVQNYVDIYKAITGKPLDKHELIRQSERVYNFQKVFCMRFGKGRRIDDVPPYRAV
                     GPVTEEEYLSRQERYDKALKEKQNIDPTGLSTQDKMKLLRQYREDQYQQLIDAVYRRK
                     GWTPEGVPTLEHLKSIGMDLPEVIAVVSKHLK"
     gene            7073..7480
                     /locus_tag="PLO35_07750"
     CDS             7073..7480
                     /locus_tag="PLO35_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017187396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOH79602.1"
                     /translation="MQENTSITGSLHHVEIYVRDLKISREFWNWLLAKLGYTVFQEWD
                     AGVSFIKGDCYLVFVQTEERWLEPRYHRCRTGLNHLAFHGGTREFIDSITRDLETRGV
                     SILYRDRHPFSNGPDCYAVFFEDPDRIKVEITL"
BASE COUNT         1952 a         2133 c         2066 g         1728 t
ORIGIN      
        1 gagcgcatga ctggcagtca tgaggtcaac ggttcgatcc cgttatgctc caccacatgt
       61 taaacccctt caccgcagcg ctgcggtgaa ggggatactt atttaagcgt gattttgagg
      121 aaagcatgag caaacacgaa caagacacaa tcgccacgct gcaagcccgt tccaaggcag
      181 cgcgtgccgc aatcctgacc atgaccactc tctcagccag cggtcatccc ggtggctcga
      241 tgagcgccat agatctgtta aactgccttt accacactat tcgtcacgat ccatcccagc
      301 cacttctgga atcgcgtgac cgggtcgtgg tgtccaacgg gcacatctcc ccggccgtct
      361 atgccattct gggattgcaa ggatatttcc ctctggatga agcgattagc cagttccgcc
      421 tggctggcag ccgttacgaa ggacacatcg aacgcgaagt tcccggcgtc gaatggtcga
      481 ctggcaattt gggacaagga ctttccgcgg gagtgggaat ggctctggca gcccgcgtaa
      541 aacaaagcga gcacagagtt tatgtgatga tgggggacgg cgagcagcag aaaggccaga
      601 tcggcgaagc cagacgcctg gccgtcaagt atcagctttc aaacctctgc gccatcatag
      661 actacaaccg tctgcagatc agcggctcca tccatgaggt gatgcctcaa aacatccgca
      721 gaaattggga agcggacggt tgggcggttt tggaagtaaa cggacatgat tttgagcaga
      781 tcctcgagac cctggaacaa gcccgccggc aggataaacc tgtgatggtg ctggcccaca
      841 ccgtgatggg aaagggggtg gactttatgg agaatctggc caaatatcac ggctccaccc
      901 tgaatcccga acagcttgac acagcactga agcagctggg cgagcccaac cggcttgaag
      961 ctctcaaagc tctgcgccag aagacagagc tgcccgtcag cgggaattgt gacatgagct
     1021 ttgccctcaa agccgatctg cagggcggcc aaccgattgt gtatgaaaaa gacacagaca
     1081 accgcagcgc ctggggaaat gccataacca acctggctga gatcaacaag agctccccca
     1141 ctcccatcgt ggtactcgat tgcgacctcg cttccagcgt caagaccaac gattttgccg
     1201 ccagatttcc ggagcgtttc ttccagggag ggatcatgga gcacaacacc gctgtagtgg
     1261 cgggtgctat atccagctgc gggatccaga ctttttgggc ggaatttggc atgtttggcc
     1321 tcgcggaagt gtataatatg cagaggctca acgacatcaa ccacagcaat ctgaaggtgg
     1381 tgctcactca cgtgggcctg gacgtcggag aagatggcaa gacccatcag agcgtggatt
     1441 acatctctct ggtccgcaat ctgcacggct tcagactgat ccttcccgca gaccccaatc
     1501 agaccgacag gatcatccgc tggctgatca acaagcccgg aaactacatc gtggcgatgg
     1561 gacgctccaa gctgcccatc ctcaagaccg aagacggtga aatctttttc aagatggact
     1621 ataacttcga gtatggcaaa gccgatctgc tcagagtggg aaaccacggc accgttctgg
     1681 tgacaggaac gccttccgcc agagccctga aggccgtgga caagctccgc gagcagggga
     1741 tttatcccca gttgtactat gtctccagtc cctgtgatct ggagcggtcc ctgctggatg
     1801 agattgccag agccaaaaga gtggtcacca ttgaagacca taacgtcaag ggcgggttgg
     1861 gcagcatcct ggccgaccgt ctggctgaag agggtctctg cgcgagcctg cagaagatcg
     1921 gggtgaggga ttatcccgtt tccggtgcgg ctgatgagct ttataagcgc gccggtatgg
     1981 acccctcgac tctggcggaa aagatctcag cctggataca ggggcccaga tagcgaaaca
     2041 agctcgcaga ttttgaaggc aggcttgttt cccggagatt acacggatgg gcacgcagat
     2101 ctcgcagttg agtgtaagat tgaggttatt gagaaggcag ggacttaaac tcacaaaacc
     2161 ctattgaatt cagcgaaatc tgcgcaacta tctgcgtaat ctgcgggaaa cttgtcccac
     2221 ctctcaacct tcaaaatata tcgcagagcc actctggagc tcaacgcgga aaaaaaagtg
     2281 ttgacagaat gcaggatacc tccatcgtga ctgaatatta catggaggag aaatgaagag
     2341 actattggtt ctttattttg ccctgctgat ctctctcagt tgtcaggctg ggcgaaaagc
     2401 gttggttata ggcaatgcca tctataacgt caatttactt tccagccctg ccaaggatgc
     2461 cgagcttgtg gcagagactc ttacgcaact gggttttacg gtcaaacatg tagccgatat
     2521 tgactacaat accttcgacg cggaactcaa gaacttcaca gggagtttga gcggtggcga
     2581 tgaggcgttt ttctatttcg gtggcatcgc tttgcagttg gagggggaaa actacctcct
     2641 gccgacaggc ttcaactaca agtccgccaa cgatatccgt ttcttcagca tccccctcaa
     2701 gcgagtgctg gaagatatgt ccaaagccaa ctatgccatg ctgtttgtgg atggaagccg
     2761 tgaaaacagc ttctcgacgt tggccggagg ccagattgga ctggctgaac ccgctgccgt
     2821 tccacccaac gcgatcctcc agtttagcac tgctccaaac gtttggctcg aatccgatct
     2881 cttgcccacc agcaaatacg ctcagtctct cgtaaatcag ttgaggatct ccgctcaaaa
     2941 catggaaaag atctgcagcg ccacgtccga aacggttgcc agacttacag ctgaggcaca
     3001 gatcccctgg acggttggct ctctgccaaa ctactattat atcaacaaca atatcatcaa
     3061 cgacggtggt tctgggctgg atgattccat cgttgcggac aatatgacct tcaaagagat
     3121 ggacctgcaa ccgggaatcg tcacttccac cggtttggaa tccagccccg cgcccgtgat
     3181 ctacactggc gacgatgccc ataagctata taaaaccagc aagttcggca atatcatcga
     3241 cgtcttttcc gcttccgagt ttatcatgac ccaatctaaa gactccaacg atctgtccca
     3301 gtttgccagc tggaatatgg tccgcctgaa tcttgccatg tttggtgccg aagcctatta
     3361 caaaaagatg ttggtctggt ataaaggggc aggtactcaa ggttccggcc tgggcgtcaa
     3421 gcaaaccgac attacccaga tgggtgcggg agccggactg caaaatccag acttcatcag
     3481 gctctttgcc acgatgaata aatacgaata cagtgatccg aacgtcaatc ctctttgggg
     3541 agaagatctt gctgactact acaactacag cttcagtttt gccaagcgcg tgagggccaa
     3601 ccggcagagt ctgcagctcg gcgcggattt tctttacaat ccagaaggtt atccggaagc
     3661 cttcctattt cagacaagcc ctttcccgca agcttacagc tttgacgatt gggatttcgc
     3721 ccattacccc tcctggaaac tgagctccta tctctatacc accgatgtct atacggatgc
     3781 ccatgaatct ctgcacctgg caggcgtcgc tcccaaggac agacctctgc tgttcaccag
     3841 gcaagacggg gtgctgtttg gcctcacctt caatatggaa gagcgcagcg ggcaatccta
     3901 cagcatggaa gattaccagc gctaccagtt ctcggttttg ctccacaaga attttggcaa
     3961 agcggtaggc ttcgatttca attttgatta caacgacagc ctgatgctgg ataccgacga
     4021 acattataaa aacaatcagt tccgcggaac gatgcgtctc accctggtgg acgcgggtcc
     4081 cctggctttg tcgaccgcga tcgattatgt gaaccgtggt cagttgtctt cagggacgga
     4141 tcccgatgag gccactccgg aacggatgca gggcatcaat ggcgctgcct tcctaatcct
     4201 cgatatatca aagcgtttga ttatttccgg gatgggacag attaacatga actggtttcc
     4261 cagcgataca ctttttaaac tggaacagac ggaattcttc tttggtggaa cggtgggact
     4321 gaggctgtga cctcagctta agcctatcca gaccatccga acctggcaga tggacaacac
     4381 ctcttccaga aaaaagagat tgacctaatc tcaaccccgg atttgtctgg ctcaaaatct
     4441 taattcctta aaggagcggg taatgaagaa attcttgaaa accatgccgg atcagtgcat
     4501 cgcttgccat gtctgcgaaa gcacctgttc caacctgtat ttcaagaccg atgacgcgga
     4561 gctctcctgc atccgcatca ccgaaacaga agaccttccg cacatgaacg cgtgcaacca
     4621 gtgcgaagct tgtgtcaaag tgtgccccac caaagccctc accgtcagcc ctcagggcgt
     4681 ggtgctggtg aataagaagc tgtgcatcgg ttgctatatg tgcatcgctg cctgccccac
     4741 ggggtcgatg ttccgttctc agggcggcct ctatcccttc aaatgcatcg cctgcggagc
     4801 ctgcgtaaaa acatgcccgg cggacgcgat ccgcgtagtg caggaataag gagatcagca
     4861 tgcaaaaacc attgcgtaaa gtattggctg agttcaacta cgagctcgct cccgtcatca
     4921 aaggctacaa ccgtcgcacc ctctacatca acgtgggaga gaaggccatc aaatccaagc
     4981 ccgtcaccga tatgatgata gacaagtttg tgggcggcaa gggttttgac ctctttctga
     5041 tgtggcacgg cgtgcaggac gacacccatt gggacagccc tgaaaacgaa atctgcatct
     5101 cctttggtcc cttgtgcgga aacaccagct accccggcag cggaaaatcc attgttacca
     5161 ccatttctcc gctcaccggc atcccggtag actgcaatgt gggtggccac tttggtccgt
     5221 attccaagtt ttccggctgg gacgccatcg agatacaggg caaggccgac gaggaagtga
     5281 tcgtctatat cgacggcgac aaaggcattg tgcagatcct cagcgctccg gatgaggaaa
     5341 tcaacagcca tatcctggca gaacgtctga tcgaagaatt tgccgacgat cccgaaaagc
     5401 atcaattcgt gtcggtggtt tcttccggaa agggagccga acatgtgcca atctcctgtc
     5461 tgaacttcag tttctgggat aaacgccgca aagtggccag gctcaaacaa gctggacgcg
     5521 gtggcaccgg tatggtattc agggacaaga agataaaggc cctggtagtc aaatattccg
     5581 gcctcaaagg cgacaccaac aatcccgtcg atttggagac cctgcaaaag accggcctca
     5641 agcttcacaa agagattgag gcaggagatt ccactcagtg ccacatgcgc gaaattggca
     5701 ccgctcacct gatggaaatc atggatgatt atgacctcct gccgatccgc aatttcaagt
     5761 atggccaaat gccggaagcc agggccctgg cctcctggga gtttaaaaat ctcttcacgc
     5821 aggggatggc agacggctgc tggtatggct gctcactttc ctgttgcaaa gctgtggaca
     5881 acttcgtgcc ccgcagcggc ccctataaag gtcataaagt ctgtgtggac gggccggaat
     5941 acgagaccgc cgctggatgc ggttccaatc tttgcatctg ggaccccaaa gacgtggtgg
     6001 agctcaattt ctactgcgac acctatggca ttgacaccat atctttcggc accagtgtgg
     6061 ctttcgccat ggaatgcttt gaaaacggga ttctcaatct ggaacgcacc ggtgggattg
     6121 atctcagttg gggcaatgcc gacagcgttt ttgagcttat ccaccagatg gcccggggtg
     6181 aaggatttgg cgtgaccgta ggccagggcg tccgcaagat gaaagatatc tttgcccgtg
     6241 agtatggtgc cgatccggct tttctgcagg atatcggcat ggaagccaaa ggcctggaat
     6301 attcacaata tatgagcaag gagtcgctgg cccagcaggg cggttatacc ctcgctctca
     6361 aaggacccca gcatgacgaa gcctggctga ttttcatgga catggtcaac aaccacatcc
     6421 ccaccttcga agacaaagcc gaagcccttc actacttccc catgttccgc acctggtttg
     6481 gcctgcaggg actctgcaaa ctgccctgga acgacatcga gccggccaac aacgccgaga
     6541 ccgacgaacc tgccaaggtc cctgaacacg tccaaaacta tgtggatatc tataaggcca
     6601 tcactggcaa acctttggat aaacatgagc tcatccgcca aagcgagcgg gtctataact
     6661 tccaaaaggt cttctgcatg cgctttggca aaggccgccg catcgatgac gtgccaccct
     6721 atcgcgctgt cggaccggtc actgaagagg aatacctctc tcgtcaggag cgttatgaca
     6781 aagctctgaa agaaaagcaa aacatcgatc ccaccgggct ctccactcag gataagatga
     6841 aactgctgcg ccagtatcgc gaagatcagt atcagcagct catcgatgcc gtctatcgcc
     6901 gtaaaggctg gactccggaa ggcgtgccca ccctcgaaca cctcaagtcc atcggaatgg
     6961 acctccccga ggtaatcgcc gtggtgtcca agcacttaaa atagcatccc gaaataaaca
     7021 tccggaacgg cggtgggctt ggctcgtccg ccgttctttg tatgaggagc ccatgcaaga
     7081 gaatacttcc atcactgggt ctctccacca cgtggagata tatgtgagag acctgaaaat
     7141 cagccgcgag ttttggaact ggctgctggc caaactgggc tacaccgtct tccaggaatg
     7201 ggacgcgggt gtaagtttca tcaagggcga ttgttatctg gtattcgtcc agacggaaga
     7261 acgctggctg gagccgaggt atcaccgctg ccggactggc ttgaaccacc tggccttcca
     7321 tggcgggacc agggagttta tcgattccat caccagggac ctcgaaacca gaggtgtaag
     7381 catcctctat cgggaccggc atcccttttc caatgggccc gattgctacg cggtcttttt
     7441 tgaagatccg gaccggatca aggtggaaat cacgctctga gccgccacta tccagctatc
     7501 ctggccacct ggcccctatc tcccatcttg tgcccctcct ttactcctcc tttgccaatg
     7561 taagggagag gcaaaggaga ggcataggtg gtgcaaagga ggctgacagg aaagcgttga
     7621 cgctggcttc tgagtttgtg cgaaaaggtc aacttggagt tcaaagcccc tggacagaat
     7681 ctgcggttag tacattgtat gaaggggctt tggacttacg tgcagtacat cgtaggaaat
     7741 tgtcttgcgg gtgtgctggt tgaagtccaa gcggcccgca aactcgctgt catgtatagc
     7801 tttatcgtgg ccgcttgaac tccgacgggg caggagcgag tggcatcact tggagttcaa
     7861 agcgcttgga taacataca
//
LOCUS       DAOSFN010000121         7776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_8100_length_7776_cov_8.251133, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000121 DAOSFN010000000
VERSION     DAOSFN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8100_length_7776_cov_8.251133"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..1427)
                     /locus_tag="PLO35_07755"
     CDS             complement(435..1427)
                     /locus_tag="PLO35_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOH79603.1"
                     /translation="MNIRLCLSILIAGCLLSGLAAEAKRPVWLSNPYQLFPEDEYICG
                     TGGGADLEAAKIQALGDISRYFESQVTAYVQTTETESSQSVNGQSTTTLGSDYQAQVK
                     VFSDVQLEYAVLADSWTDPETKLVYVLMTIEKLKLRAKYLERVNSSEQAIGQHLKVQG
                     NPFQRYSALLKATRLMPDLLRDCNFYNTLISSGAPRLQASLSEASLQALIAEARAAIV
                     FDISVPDDPQGSIATTLQTAIQKMGFGFGPSALKMDVRMAPTENKQLGKQYFVGYEAV
                     LTLSHEGTQIFVTKESSKQGDVDEGSARNRTLKALSSGLANKFTRELNKYIEGL"
     gene            complement(1446..2186)
                     /locus_tag="PLO35_07760"
     CDS             complement(1446..2186)
                     /locus_tag="PLO35_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79604.1"
                     /translation="MIKKLVIPVLITVLLLSACGSNRKEAKPKKPGSKADKQALLLDE
                     AMLADESLAAVYAGQLVLQATDCLLQSVMYGGSSDFPSLSQAFDLSKNHVSWSEETAW
                     QAFGLRQMVVAARAEASAQGAGLAGSEEALQGENLQAQMDAKLKEGVQLQDSQRDFFL
                     ASLIYLAAAVVKETNISKNLPDYISRAKSLKGFDALREAQYLPKALALMSGLPGMIAS
                     QTMTLKTYLDIAKTNNIEIPSDVKSMLP"
     gene            complement(2196..3179)
                     /locus_tag="PLO35_07765"
     CDS             complement(2196..3179)
                     /locus_tag="PLO35_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79605.1"
                     /translation="MRKLLIILGLLLILSAGTEQLDALVKVKAKGSVATKNKKPKTEE
                     RQQAILNGQQQALRKYASGLDSQRLSILNELMPELLKDIEKYILDTTPLNDGEYQNGN
                     WEINLEVSIDDAQIEQLVRQKTPKLEEHFLSFVYVAREVASVEFHEGTVSIQADTDRD
                     HFKLDGSIVMNSEGISYRSYNPAEIDSRVTEIFNKAGFDVVPAFEVNLTPEQFAYDFI
                     TLDEISNSTQKEATDIARDQGLEFLALGTLDIGREEIDPVTGQYKIYAKVGGYIMDLR
                     KKFAVKICSVGPLQYSGLGPNPTVAKTNALIEAATKASQDLVDQLRVKLTP"
     gene            complement(3164..4264)
                     /locus_tag="PLO35_07770"
     CDS             complement(3164..4264)
                     /locus_tag="PLO35_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79606.1"
                     /translation="MDKRLVIIGIMLLLVTLLGAQERVRYAGFCFAGNYADIPLNYPH
                     THALSAGEKGAPNRLEQLFYSYLHANRDFRNFSLDLEQSSQSDKALAITINRESVDAE
                     SISGQTKLIYNLSYSIYILDFRELKVLQSFPFKISYIELYQQPPSATEIQSQIYKLME
                     EELLASIKSKQPQIAIRQPGSLAMKVANVEISEDAVALLGPYEDKLDSYKAILANQLT
                     ETFAFGLNVTMLPYAKDYAGQKMALAFSDARVLNFSIPPASYDLDLRLDKFHKALYKE
                     TAFERVDIYGAYINLKIYDAELETVYWDQAVKFGATKQTVNGQLTDDFANYHEVLLQT
                     IGTELTNTIRNDKKLMENKKNKKGVIIRCANY"
     gene            4813..7593
                     /locus_tag="PLO35_07775"
     CDS             4813..7593
                     /locus_tag="PLO35_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOH79607.1"
                     /translation="MFQAIGSPTLILDTSFNILHANRAATSATGKSAEQLRGKKCFTV
                     FHSPDHKQQVKGCPMASLLATSQAQAKEMEVETLGGTYLVSCTPVLDEKNAVSQIIHI
                     ATPITEHKRVANELERQNRFLTQLNEFAIKLSHLGDQSDLEAMIATELQKISGAVVLT
                     ISKYDPGSKCLYVTKLQGDSWILNQVNRTIQAEVGKIPSPLSDKEYQEIISEQVGVRK
                     SLHDASFGGVSKLVSGALGRLLNIKHYLGIAYVCDNRLFGTSLLAMQKGTPDPPFEIL
                     RTFGQLAALALQRKQASEALRESEDKYRQLIETMSEGVVVVDNDDVIQFVNPAACKMY
                     GYREQDLTGQTGYKLLVDESDWDTVKYKNTLRQNGITDSYLVRGKKLSGDLIWIRISG
                     TPILDKSGAVAGSVGILSDVTQLKQNEEALLMQNAYLDELIEGAAEGIVVLDNDEVVL
                     RVNKEFTRIFGYSLEEARGKRINDLIVPDHLRDEGWNATSTVARGDRVYFETVRKHKN
                     GSLIDVSVLGNPIRLENNQIGVYGIYRDITDRKKLEEQLHHSQRLDSIGHLAGGVAHD
                     FNNMLTVILGYGEQLLDAFGPGHPLRKDVGEILNAGNRALKLTNQLLAFGRKQMIRPQ
                     VISLNQVIDDMHSMLERIITEDIEIETVLSPDLHDIKADTSQIEQVIMNLVINACEAM
                     PRGGKLSIQTTNDTLVPEDARIHAFTRPGEYVLLCITDTGIGMDKETLTRIFEPFHST
                     KDRTKSTGLGLATVYGIVKQADGYIWVYSEPKHGTSFKILFPACFDVREQPPHKSLKP
                     LLNGGGQQVLVVEDEDMLRDLITTIIQKLGYSVTAVSNGEEALEIVREKGLEPVLTVT
                     DVVMPGINGKELADLLCENHPDRKVLFMSGYTESIIAHRGIIDDSIQFIQKPFTQAEM
                     AEKIQHLLGIGN"
BASE COUNT         1806 a         1980 c         2078 g         1912 t
ORIGIN      
        1 attccccctt cggtgtactg gttgaagtcc aagcggcccg caaactcgtt gtcattgaaa
       61 cctttaccat ggccgcttga actccaacga gcccatttca cactttggct tcgccagttt
      121 ttgccctagc tcaaaaacca gaggtacata tatgcgcaac atccttttat aacattatca
      181 tcggccctta ccaattcact ggcgtgacct tggccaggag caagccggac ccaaaagcca
      241 tttatggggc ttggcccggt tttggtctcg ggctgcttag cttcagcgat tcagagcgaa
      301 atgagtaatg gaccagattt ccccaaccgg cctccacttt tccacccgct catcacactc
      361 tcaccaccta tgccgaggca taggagaggc aaaggagatg taaaggtggt gtaaaggagg
      421 ctaaggggaa aggatcagag gccttcgatg tatttattga gttcgcgggt gaatttgttg
      481 gccaggccgc tggacagggc tttgagggtt cggttgcgtg ctgagccttc atccacgtca
      541 ccctgcttgc tgctttcttt ggtgacgaat atctgcgtgc cttcatggct gagcgtcaat
      601 accgcttcat agcccacaaa gtactgtttc ccaagctgtt tgttctcggt gggggccatc
      661 ctgacgtcca tcttcagagc gctcgggcca aagccgaaac ccattttttg gatggcggtt
      721 tgcagggtgg tggctatact gccctgggga tcatctggaa cgctgatatc gaagacgata
      781 gccgctctgg cctctgcgat cagggcctgc agggatgctt cagagagcga ggcctgcagt
      841 ctgggagccc cggagcttat cagcgtgttg taaaagttgc aatccctcag cagatcgggc
      901 atcagccttg tggccttgag cagagcgctg taacgctgaa aaggattgcc ctgcacctta
      961 agatgctgtc ctatcgcctg ctccgaggag tttacccgtt ccaggtattt ggcccgcagt
     1021 ttgagcttct cgatcgtcat caacacgtaa acgagcttgg tctcgggatc tgtccagcta
     1081 tccgccaaca cggcatattc cagctggaca tccgagaaca ccttcacctg ggcctgatag
     1141 tcactgccaa gggtggtagt gctttggccg ttaacgcttt ggctgctctc agtctcggtg
     1201 gtttggacat aggcggtcac ctgggattca aagtatcggg agatatcccc caaggcctgg
     1261 attttggcgg cttccagatc tgccccgcct cccgttccgc agatatattc atcttccggg
     1321 aagagctgat agggattgga aagccagaca ggacgttttg cttcggcagc cagtccgctt
     1381 agcagacagc cggctataag tattgacaga cagagtctta tattcatttc tactatcctc
     1441 ctgcctcagg gcagcataga cttcacatct gaaggaatct cgatgttgtt ggtcttggcg
     1501 atgtccagat aggtcttcag agtcatggtt tgagaggcga tcattccggg taatcctgac
     1561 atgagagcaa gagctttggg cagatactga gcttcccgca aggcatcgaa gcccttgagg
     1621 gattttgccc ggctgatgta atccggcaga tttttggaga tattggtctc tttgaccacg
     1681 gccgcggcca gatagatcag agaggccaga aagaaatccc gctgggaatc ctgaagctgg
     1741 acaccctctt tgagcttggc atccatttgg gcctgaaggt tttcgccctg caaggcttcc
     1801 tccgaaccgg ccagtccggc tccctgagcg ctggcctccg ctctggcggc caccaccatc
     1861 tgtcgcagtc caaatgcctg ccaggcagtc tcttccgacc aggatacatg atttttggat
     1921 agatcgaagg cctgggacaa actgggaaaa tcgctgctac cgccatacat gacggactgg
     1981 agcagacaat cggtggcctg gagtaccaac tgacccgcgt aaacggctgc caggctttca
     2041 tctgccagca tggcttcatc cagcagcaga gcttgtttgt ctgcctttga gcctggcttt
     2101 ttgggtttgg cctctttgcg gttcgatcca caggctgaga gcagcagcac tgtgatcaaa
     2161 accggaataa cgagcttttt gatcatcgcg tcctcctaag gcgtgagctt cactctgagc
     2221 tggtccacca aatcctggga ggctttggtg gctgcttcga tcagggcatt ggtcttggcg
     2281 acggtgggat tgggtcccag gccgctgtat tgcaggggtc ctacgctgca gatcttgacc
     2341 gcgaactttt tgcgcaggtc cataatgtag cccccgactt tggcgtagat cttgtattgt
     2401 cccgtcacgg ggtcgatttc ttcgcgtccg atgtcaaggg tccccaaggc caggaattcc
     2461 agtccctggt ccctggcgat gtcggtggcc tctttctgag tggaattgga gatttcgtcc
     2521 agggtgataa aatcataggc gaactgctcg ggagtgaggt tgacctcgaa ggcgggcacc
     2581 acgtcaaagc cggctttgtt gaagatttcc gtcacccggc tgtcgatttc ggcgggattg
     2641 tagctccggt agctgatccc ttccgagttc atcacgatgc tgccgtccag cttgaagtga
     2701 tctctgtccg tgtcggcctg gatgctcaca gtaccctcat gaaactccac gctggctacc
     2761 tctctggcca catagacaaa gctgagaaag tgctcttcaa gtttgggggt tttctgcctc
     2821 acgagctgtt cgatctgggc atcgtcgatg ctcacctcca ggttgatctc ccagttgccg
     2881 ttttgatact cgccatcgtt cagaggcgtg gtgtccagga tgtatttttc gatgtccttg
     2941 agcagttccg gcatcagttc attcaggatg ctgaggcgtt gggaatcaag gccggaagcg
     3001 tattttctga gcgcttgctg ctgtccgttc aggatggcct gctggcgttc ttccgtcttg
     3061 ggtttcttgt ttttggtggc cacactgcct ttggctttca ctttcacgag cgcgtcgagt
     3121 tgttccgttc cggcgctcaa gatcaacagc aagcccagga taatcagtaa tttgcgcatc
     3181 tgatgatcac cccttttttg tttttcttgt tttccatcag ctttttatcg ttgcggatgg
     3241 tgtttgtaag ctctgtgccg atggtctgca gcagcacttc gtgatagttg gcaaagtcgt
     3301 ccgtcagttg gccgttcaca gtctgtttgg tggcaccgaa tttgacagcc tggtcccagt
     3361 acacggtttc cagctccgcg tcatagatct tcaggttgat ataagcgcca tagatgtcca
     3421 cccgttcaaa ggccgtttct ttatagagcg ctttgtgaaa cttgtccagc ctcaggtcca
     3481 ggtcgtagct ggcgggcggg atactgaaat tgagcactct ggcatcggaa aaagcgaggg
     3541 ccattttctg gccggcatag tctttggcgt atggaagcat cgtaacgttg aggccaaagg
     3601 caaatgtttc ggtgagctga ttggccagaa tcgctttgta gctgtccagt ttgtcctcgt
     3661 agggacccag cagagccacg gcgtcctcgc ttatctccac gttcgccact ttcatggcca
     3721 aagatccggg ttggcggatg gcgatttggg gctgtttgct cttgatggaa gctaacagct
     3781 cctcctccat cagcttatag atctgggact ggatttctgt ggcggacgga ggctgctgat
     3841 aaagctcgat ataactgatc ttgaagggga aggactgcag cactttcaac tccctaaagt
     3901 caagaatata aatgctgtag ctgagattgt agatcagctt ggtctgtccg gaaatgctct
     3961 ccgcgtccac gctttcacgg ttgatcgtaa tggccagggc tttgtcactc tgagagcttt
     4021 gttccagatc gaggctgaaa ttgcgaaagt cccggttggc atgcaaataa ctgtagaaca
     4081 gctgttccaa acggtttggg gcccctttct caccagcgct cagggcatgg gtgtggggat
     4141 aattgagagg aatgtctgca tagtttccgg caaagcaaaa accagcatac ctcacccgtt
     4201 cctgagcacc caaaagggtg accagcaaca gcatgatccc tatgatcacc aagcgtttat
     4261 ccaatttcca caacctccag cttgtcatca cggcttccag accggccccc gcatcgacgt
     4321 gcggaccaat ccaagccgcc gatcattttc cagcctgact tccagcactg taaagacgac
     4381 aaatgcatct ttggccctgc ggcgatattg tcaaccgctt ttttcccgga catgggtgat
     4441 agggcctctc ttttggtatc aagaacctgc ctgatttgcg gtctggtgtg aggcagtgaa
     4501 tgcttggata ccagagcaaa aagctctgaa aaaagcgttg acataggctg gtaaatggct
     4561 tatcttctgt tcatggtgct gatttgtatt agcaggttca cccagtagat ataaggagtc
     4621 tctatgccga aacgagtcat acgcttgttc aatcccggaa cgcggattcg tcctgttaac
     4681 gaagggggca tagctcttct actcacattt cataatgccc acggtgacag acagacagcg
     4741 cggtgtatcc atgacgcgca gtcttttgat gaccccgctc ggccagatca cgctcgcctt
     4801 aactgggaaa gtatgtttca ggcgatcggc agtcccactc tgatcctgga taccagtttc
     4861 aacatcctgc atgccaatcg ggccgcgaca tccgcgacgg gtaagagcgc ggaacagctg
     4921 cgggggaaga agtgttttac cgtgtttcat tctcctgatc acaagcaaca ggtaaaaggc
     4981 tgcccgatgg catccctgct tgccacaagt caggcacagg ccaaagaaat ggaagttgag
     5041 accctgggcg gcacctatct ggtatcctgt acgcctgttt tggatgagaa gaacgcggtg
     5101 agccaaatca tccatatcgc cacaccaatc accgagcaca agcgagtggc taacgaactg
     5161 gaaaggcaaa accgcttttt gacccagctg aacgaattcg ccatcaaact tagccacttg
     5221 ggagatcaaa gcgacctgga agccatgatt gccactgaac tccagaaaat cagcggcgcg
     5281 gtggtcctga ccatctcgaa atacgatccg ggcagcaaat gtctgtatgt taccaaactg
     5341 caaggcgatt cctggattct aaaccaggtg aaccggacca tccaggcaga ggtgggaaaa
     5401 atcccttcac ctctctcaga caaggaatat caagagatca tctctgaaca ggtgggtgtc
     5461 cggaaatctc tgcatgacgc gtcctttgga ggcgtttcta aactcgtcag tggagctttg
     5521 gggcggttgt taaacatcaa acactatctg gggattgctt acgtctgcga caaccgtctc
     5581 ttcggcacct cgctgctggc tatgcaaaag ggaacgcccg atccgccctt tgagatcttg
     5641 cgcacctttg gtcagctggc cgctctggct ttgcagcgca agcaggcatc cgaagccctg
     5701 cgggagagtg aagataaata ccgccaactg attgaaacca tgagcgaagg cgtggtcgtg
     5761 gtcgataacg atgacgtgat ccagtttgta aaccctgccg cgtgtaaaat gtatggatac
     5821 cgggaacaag atttgactgg ccagactggt tacaagctgt tggtagatga aagtgactgg
     5881 gacactgtca aatataaaaa cactctccgc caaaacggaa tcacggattc gtatctggtg
     5941 agaggcaaaa aactgtccgg agatttgatc tggatcagaa tcagcggtac tcccattctg
     6001 gataaatccg gagcggtagc aggatcagtg ggcatcttga gtgatgtgac ccaactgaag
     6061 caaaacgaag aagccctgct gatgcagaat gcttatctgg acgaactgat cgaaggggct
     6121 gccgaaggca tcgtggttct ggataatgac gaggtcgtcc tgagagtaaa caaggaattc
     6181 accaggattt tcgggtacag cctggaagaa gccagaggaa aacgaataaa cgacctgatc
     6241 gtcccggacc atctgagaga tgagggctgg aacgcgacat ccactgtagc gcggggcgat
     6301 agagtgtatt ttgaaaccgt gcgcaagcat aaaaacgggt ccctgatcga cgtatctgtc
     6361 ctgggaaacc ctatccgctt ggaaaataat cagatcggcg tgtatgggat ctatcgggac
     6421 atcaccgacc gcaaaaagct ggaagaacag ctgcaccaca gtcagcgtct ggattccata
     6481 ggtcatctcg ccggtggggt agcgcacgac tttaacaaca tgctaaccgt gattctgggt
     6541 tatggggagc agttattgga cgctttcggg cctggccacc ctctccggaa agacgtggga
     6601 gaaatcctca acgctggtaa ccgcgccctt aaactgacca atcaactgct ggcctttggc
     6661 agaaaacaga tgatccggcc ccaggtgatt agcctgaacc aagtgattga cgatatgcac
     6721 agcatgttgg agcggatcat caccgaggat atcgaaatag aaacggtgtt gtcgccagat
     6781 ctgcatgata taaaagccga taccagccag attgagcagg tgatcatgaa tctggtgatc
     6841 aacgcttgcg aagctatgcc acggggtggc aaactgagca tccagaccac gaacgatact
     6901 ctggtaccag aagatgctcg tatccacgct tttacgcggc ctggcgaata tgtgctgctc
     6961 tgcatcaccg ataccgggat cggaatggac aaggagactc tgacgagaat ctttgaaccc
     7021 ttccacagca caaaggacag gacaaaaagc acgggtctgg gactggcaac cgtctatggc
     7081 attgtaaaac aagcagatgg atatatctgg gtgtatagtg aaccaaagca tggcaccagc
     7141 tttaaaatcc tctttccggc ctgctttgat gttcgtgaac agcctcccca taaatcgctc
     7201 aaaccccttc tgaatggtgg cggacaacag gtcctggtag tcgaggacga agatatgctc
     7261 agggatttga tcaccacaat aatccaaaaa ctcggctata gcgtaaccgc cgtatccaac
     7321 ggtgaagaag ctctggaaat cgtcagagaa aagggtttgg aaccggtttt gactgtcacc
     7381 gatgtcgtga tgcctggaat caacggcaaa gaactggctg atctcttgtg cgaaaaccac
     7441 ccggatcgaa aagtgctgtt tatgtctgga tatacggagt ccatcattgc gcatcggggt
     7501 ataatagatg acagcatcca gttcatccaa aagcccttta cccaggcgga aatggcagag
     7561 aaaatccagc atttgctggg tatcgggaac tgatccaccg gaagctgtcc atcattaggt
     7621 ggggcatttt ctgccaaaat tggcgccagg gtctttaatc gggcctgaaa gatttccagc
     7681 gattagcttt gcacataggg tacctgatcc tgggctgaat acgttttgat tttttagcag
     7741 tctcactttt agattgacaa ttttcctgtg tcgatt
//
LOCUS       DAOSFN010000122         7635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_8295_length_7635_cov_9.262401, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000122 DAOSFN010000000
VERSION     DAOSFN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8295_length_7635_cov_9.262401"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1119
                     /locus_tag="PLO35_07780"
     CDS             199..1119
                     /locus_tag="PLO35_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH79608.1"
                     /translation="MPKVLIATEKPFAAEAVSKIKAELDQAKYAYEFLENYTEAAQLH
                     AAVADAEAMIIRSDIADEAVFNAAKKLRIVVRAGAGYDNIDLKAATAHDVVAMNTPGQ
                     NSNAVAELAIGLMIYMARGKFNGKSGSELAGKKLVLYGYGFVARYLAKMAKGIGMEIF
                     AYDPYLTNEIMTKDGVTPLASVADIFKTGDFISLHIPATAETKKSINWDLLSLMKNNA
                     ILVNTARKEVIDEEALVKALEEKKKFRYVSDVEPGNSAELAEKFADRVYWTPKKMGAQ
                     TEEANINAGVASARQIIAFFEKGDKTYRVN"
     gene            complement(1194..2291)
                     /locus_tag="PLO35_07785"
     CDS             complement(1194..2291)
                     /locus_tag="PLO35_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOH79609.1"
                     /translation="MLRRPKTAILILMILAATLLSAQYNSTPTPQKRVFSNEDYNDFY
                     DCIRYANELYDDVVEYIRLGIDVNRANTWGETPLHIAAYNDRPATTELLLNSGAQINA
                     KNSQGETPLWAAARYGCLDALSMLLQKGAKINTCSNAGMSALLAARIFNNDAYQMLIA
                     KAKTTDYSDLTIESLVGYRTDLREMIKNGASVDEVNSYGWNPLQISVRNPNWRGGFDY
                     LAVTMIDYSGNLEVLSPLGDNLLNLAAMGGDHWTIRHLIKKGVKVNNQNKQGETPLIQ
                     LFQYGNSSWVPLLLENGANPRLQDKNGWTPLMYAVRHWYGWDTLNRLLEAGSDLKAVN
                     KDGKTALDIAREINNKTAIDILTGYKAPYRY"
     gene            complement(2293..3372)
                     /locus_tag="PLO35_07790"
     CDS             complement(2293..3372)
                     /locus_tag="PLO35_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79610.1"
                     /translation="MRRLLLLLILLSIPLAYYGQGGGYGAYVSVGSVVIKTSVSGDIY
                     IDNKLICPITIGESKSIYDVVSGLRTIELRSPQGKLTQRVNVPSDDIVVANFIVDLTH
                     KGLVYVEGGSFTMGDTSGKYPRDERPHHTVNLSPFYIGKHEVTQQLFKEVMGYNASYF
                     CYPDNPVEMISWYEAIEFCNKLSVREGLQPVYTIDKGKKDSNNYQPDDDLKWTVTYDQ
                     FALGYRLPTEAEWEFAARGGIYGRGFLYAGSNNPKSYGWYNAYDGSYVYSTYQVGSFN
                     PNEIGLYDMSGNVYEWCWDWFGDYSAMEQNNPSGARAGSFRIVRGGSFYSDAYRLRLS
                     QRGYDKPNRYYNVIGMRLARSSGGF"
     gene            complement(3606..3872)
                     /gene="rpmA"
                     /locus_tag="PLO35_07795"
     CDS             complement(3606..3872)
                     /gene="rpmA"
                     /locus_tag="PLO35_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOH79611.1"
                     /translation="MAHKKGVGSSRNGRDSNPKYRGVKKYGSELVVAGNIIVRQKGTK
                     FHPGANVGIGRDFTIFSLIDGTVKFETKRLGRKYVSVEPLPEDK"
     gene            complement(3883..>4239)
                     /gene="rplU"
                     /locus_tag="PLO35_07800"
     CDS             complement(3883..>4239)
                     /gene="rplU"
                     /locus_tag="PLO35_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOH79612.1"
                     /translation="PSDLWFFHVKYKEKMMYAIVEIKGFQFRAEKNAILRVPLLNTEP
                     GQSVEFDKVLMLRDEADIQIGQPVIEGAAVLAEVIEHGKDKKKVIFHTHRRKGYRVKK
                     GYREQYTNIKVTDIRS"
     assembly_gap    4240..4339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4340..4920)
                     /locus_tag="PLO35_07805"
     CDS             complement(<4340..4920)
                     /locus_tag="PLO35_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOH79613.1"
                     /translation="MDKRAMIGARIARELKDGDYVNLGIGLPTEAVNHIPEGVRVIFQ
                     SENGMLGVGPKPLDGQEDQDMINAGGGFITALPGAAFFDSATSFAIIRGGHIDVTVLG
                     ALQVDQHGNLANWMIPGKLVPGMGGAMDLVTGAKKVIVAMEHCDKNGNSKVLKQCTLP
                     LTAKAKVSLIVTDLAVIEVTPEGLLLREVTEGL"
     gene            complement(4913..5578)
                     /locus_tag="PLO35_07810"
     CDS             complement(4913..5578)
                     /locus_tag="PLO35_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOH79614.1"
                     /translation="MFKLITAAEAAAMIAPHSRVAIGGFLAVGAPEGLIDAIVAAGTK
                     DLHIIVIASDWENRGIGKLVVNHQIKSAQVSHLGTNKEIQAQMNAGEIKIELVPQGTL
                     MERVRAYGAGLGGVLTPTGLGTVVEEGKQIISVDGKDYILEPAIASDFAIIRAQKADQ
                     NGNLIYRKTARNSNPIMAMAGRITIAEVDEIVPTGQLDPEQIVTPGVFVNYVVLSGEV
                     KHG"
     gene            complement(5657..6982)
                     /gene="hflX"
                     /locus_tag="PLO35_07815"
     CDS             complement(5657..6982)
                     /gene="hflX"
                     /locus_tag="PLO35_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOH79615.1"
                     /translation="MIEERDFELDEFDQEDESWEELETDEKTAFIVAIVRQNEASADV
                     EASLDELERLADTAGIMVLGRYTQKRNQPERSTYFGRGWLNEIAEKMHQAQAELLIVN
                     EELSPIQGRNIEKQFSITVIDRTEVILSIFHDHARTREARLQVKLAELEYQLPRLRKL
                     WGHFDKERGSMRAKGGAASRGMGEKQIEIDKRLIRQQIRRVTHWIETVTHQKETQRKG
                     RDKTLKLCLVGYTNAGKSTLFNALTGAGVLVEDKLFATLDSTSRQLKLSTGQPLVISD
                     TVGFISRLPHHLVASFRATLMEVRDADLLLHMVDVSDERFEYYIEQVNLVLNEIGADQ
                     LPQILVLNKIDLLDATYLTLLKRRFPWAVFISASQQDRIPDLLSRVEQALLDTRQLTL
                     HLPFDQGGLASRLHEIADIHTEEYREDGIWLQLSLSGRDLRLVEKYIVE"
     gene            complement(6998..>7635)
                     /locus_tag="PLO35_07820"
     CDS             complement(6998..>7635)
                     /locus_tag="PLO35_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79616.1"
                     /translation="MSWNTARRAEYLEIFREMLSKGPQATRQPQLSGPHNGMVATYGA
                     ARKDSAFKLNNAVKGMGFCPDEKSVDALLDLLSSHMEAGMEQKLNLLDSLYQHASEHF
                     SPHALVSLELYSEPQYNTQTFINQMVNPACVTVFLDIPTFKIKQIARLLHPMDPSLTD
                     YERKVTRYVNLMHSFFHGAFPRDYIAVIYYNVEVYDSSPGRMDARGTRLNP"
BASE COUNT         1607 a         2000 c         2045 g         1883 t
ORIGIN      
        1 ctgtgacgac agggctcaac ctttggccag cggggtgtat ctgctgcgcc tgagcgtgag
       61 tggcaagcct gccagacaga tgaaagcggt gctggttaaa tagggcttac caatcctgaa
      121 ctggcaagat ttaggttgac acctttgggc ggggcggata gaatggcttt attgaaaaaa
      181 aaccttatgg aggctctcat gccaaaagtt ctgatcgcca cagaaaagcc tttcgcggcc
      241 gaagccgtga gcaaaatcaa ggctgaactg gaccaggcca aatatgccta tgagtttctg
      301 gaaaactaca ctgaagcagc ccaactgcat gccgccgtgg cagatgccga agccatgatc
      361 atccgcagcg acattgccga tgaagccgtc ttcaatgcag ccaaaaagct caggatcgtg
      421 gtccgggccg gtgccggata tgacaacatc gacctcaaag ccgctaccgc tcatgacgtt
      481 gtggccatga acactcccgg tcaaaactcc aatgccgtgg ccgaactggc cattggattg
      541 atgatctata tggccagagg aaagttcaac ggcaaatccg gcagcgagct ggctggcaag
      601 aaactggtgc tctacggata cggctttgtg gcccgctacc tcgccaagat ggccaagggc
      661 attggcatgg aaatctttgc ctatgatccc tacctcacca acgagatcat gaccaaagac
      721 ggagtgaccc ctctggccag cgtggccgat atcttcaaga ccggcgactt tatttccctg
      781 cacattcccg ccaccgctga gaccaagaaa tccatcaact gggacctctt aagcctgatg
      841 aaaaacaacg ccatcctcgt caacaccgcc cgtaaagaag tgatcgacga ggaagctctc
      901 gtaaaagccc ttgaggaaaa gaagaaattc cgctacgtga gcgacgtcga gcctggcaac
      961 tcagccgagc tggccgagaa gtttgccgat cgcgtctatt ggacccccaa gaaaatgggc
     1021 gcccagaccg aagaagccaa cataaatgcc ggggtggctt ccgcgcgtca aatcatcgcc
     1081 ttttttgaaa agggagacaa gacctaccgg gtcaactgag cagatatttg ttctgagaca
     1141 ataaaaaacc cgtctttgga cgggtttttt gtctgtagcg gaaatcgtca ggttcagtat
     1201 ctgtagggtg ctttataccc tgtgaggatg tcgatagcgg ttttattgtt gatttctctg
     1261 gcgatgtcca gagcggtttt gccatctttg ttcacggctt tgaggtcaga tccagcctcc
     1321 agaagccggt ttaaagtgtc ccagccatac cagtggcgca ccgcatacat cagaggggtc
     1381 cagccatttt tatcctgaag cctgggattg gcgccatttt ccagcagcaa tggtacccag
     1441 gagctgttgc catattggaa aagctgtatc aggggagttt cgccttgttt gttttggttg
     1501 tttaccttca cgcctttctt gatcaggtgg cggatagtcc aatggtctcc acccatggcg
     1561 gccaggttta acagattgtc tcccagaggg gaaagcacct ccaaattgcc gctgtaatcg
     1621 atcatggtga cggccaggta atcaaagcct ccccgccaat ttgggtttcg cacgctgatc
     1681 tggagagggt tccagccata gctgttgact tcgtccacgg aagctccgtt tttgatcatt
     1741 tcacgcagat ctgtgcgata tcccaccagg gattcgatgg tcaggtcaga gtaatctgtc
     1801 gttttggcct tggcaatcag catctgatag gcgtcgttgt tgaaaatgcg ggctgccagc
     1861 agagcgctca tcccggcatt gctgcaagtg ttgatcttgg cgcctttctg caacagcatc
     1921 gacagggcat ccagacagcc atatctggct gccgcccaca ggggcgtctc accttgagag
     1981 tttttggcgt tgatctgggc tccgctgttt agcagcaatt cggtggtcgc gggacggtcg
     2041 ttgtacgcgg cgatgtgcag aggagtttct ccccaggtgt tggcacggtt cacatcgatg
     2101 cccaggcgaa tgtattccac cacatcgtca tagagctcat tggcgtagcg gatgcagtcg
     2161 taaaagtcgt tgtaatcttc attactgaag acccgtttct gaggggtggg agtagaattg
     2221 tactgagcgc tcagaagcgt cgcggcgagg atcatcagga ttagaatggc agtttttggg
     2281 cgtcttaaca tcttagaaac ctccgctgga acgcgccagg cgcattccga tcacgttgta
     2341 atagcggttg ggcttgtcat aacccctctg gctcagtctc aggcggtagg catcgctgta
     2401 aaatgatccg cctcttacaa ttctgaagct tcctgccctg gccccactgg gattgttctg
     2461 ttccatggca ctgtagtctc caaaccagtc ccagcaccat tcatagacgt tgccgctcat
     2521 gtcgtaaagg ccaatttcgt tgggattgaa gctgcctacc tgataggtgg agtagacata
     2581 agaaccatca taggcgttgt accatccgta ggacttgggg ttgttgctgc cagcgtagag
     2641 aaaccccctg ccatatattc caccccgggc cgcgaattcc cattccgcct cggtgggcag
     2701 tctgtagccg agggcgaatt gatcgtaggt cacggtccac ttgaggtcgt cgtccggttg
     2761 atagttgttg gagtcttttt tgcctttgtc aatagtgtag acaggctgca ggccttcccg
     2821 gacgctgagt ttgttgcaaa actcgatcgc ctcataccag ctgatcatct ccaccggatt
     2881 atccggatag cagaagtaag aggcgttgta tcccatcact tccttaaata actgctgcgt
     2941 gacctcgtgt tttccgatgt aaaacgggga aagattcacc gtgtggtgag gacgttcgtc
     3001 ccgggggtat ttacccgatg tgtctcccat cgtgaaggat cccccttcca catagacgag
     3061 gcctttgtgg gtcaggtcca cgatgaagtt ggcgaccacg atgtcatcgg agggcacgtt
     3121 tacccgctgg gtcaacttgc cctggggaga tctaagctcg atggttctca ggcctgaaac
     3181 cacgtcgtag atagacttgc tttctccgat ggtgatggga caaatcagct tgttatcgat
     3241 gtagatatcg cctgaaacgc tggtcttgat gaccacactg cccacggaaa cgtaagcgcc
     3301 gtatcctcct ccctgtccat aataggccag gggaatactc agcagaatga gcaaaagtaa
     3361 caatcttctc atagtttgaa acctctttcg gcaggatttc caaagcctcc aaactgtcaa
     3421 catttttcct taaccgccct tgttcattgc cccccggatc actggtagca aagccggacc
     3481 acttcggact aagctgggtg tggtcccgag gagggtgagc caaggcaggg gccgttggtt
     3541 taggaatgag taaaaaaaaa gccccgtctc catgcgaaaa cggggcttaa gctgatgggc
     3601 tgaatttact tatcttcggg gaggggctcc acggagacgt acttgcgccc cagacgcttg
     3661 gtctcaaatt tgacggtgcc gtcgatcagg ctgaagatgg tgaaatctct gccgatgccg
     3721 acattggcgc cgggatggaa cttggtgcct ttctggcgga cgatgatgtt gccggctact
     3781 accagctcgc tgccatattt cttcacgccg cggtatttgg gatttgaatc gcgaccgttt
     3841 ctgctgcttc caacaccttt tttatgtgcc atgtttcacc tcttaagacc ggatatcggt
     3901 caccttgata ttggtatatt gttcacggta gccctttttc acgcgatagc ctttgcggcg
     3961 atgggtgtga aaaatcactt tcttcttgtc tttaccgtgt tctatcactt ccgcgagaac
     4021 agcagcgcct tcgatcacgg gttggccaat ctggatgtca gcttcgtccc gaagcatgag
     4081 caccttgtcg aattcgacgc tttggccagg ttccgtgttc agcaagggaa cgcgcaggat
     4141 ggcgtttttt tcagccctga actgaaatcc tttaatttca acgatggcgt acatcatttt
     4201 ctccttgtat tttacatgaa agaaccacaa atctgagggn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnc tgaggccttc agtcacctct ctcagcagca ggccttccgg
     4381 cgtgacttca atcacggcca ggtctgtgac gatgaggctc actttggctt tggctgtgag
     4441 gggaagggtg cactgtttca acactttgga gttgccgttc ttatcacagt gttccatggc
     4501 cacgatcact tttttggctc cggtcacgag gtccatagcg cctcccatcc ctggaacgag
     4561 ctttcctggg atcatccagt tggccagatt gccgtgctgg tccacctgca aagcgcccag
     4621 gacggtgacg tcgatatggc cgcctctgat gatggcaaag ctggtggcgg aatcgaagaa
     4681 agcggcaccc gggagggctg tgatgaaccc cccaccggca ttgatcatgt cctgatcttc
     4741 ttgtccgtca aggggtttgg gccccacgcc cagcatcccg ttttcactct ggaagatcac
     4801 tctcacgcct tccgggatgt ggttgacggc ctcagtgggc aatccgatcc cgaggttcac
     4861 atagtctccg tctttgagtt cacgggctat tctggcacct atcatcgcgc gcttatccat
     4921 gtttgacctc cccacttagc accacataat tcacaaatac gcctggcgtc acgatctgtt
     4981 cgggatcgag ctggccggtt ggaacgattt catccacctc ggcgatggtg attctgcccg
     5041 ccatagccat gatgggatta ctgttgcggg cggttttgcg atagatcagg ttgccattct
     5101 ggtcggcttt ttgggctcga atgatggcaa agtcgctggc aatggcaggt tccaggatat
     5161 agtccttgcc gtccacgctg atgatctgtt tgccttcttc caccacggtg ccgagaccgg
     5221 tgggcgtcaa aacgccaccc aggcccgctc cataagcccg caccctttcc atcagggtac
     5281 cctgcggcac cagttcgatc ttgatctcac ccgcgttcat ctgggcctgg atttctttgt
     5341 tggtgcccaa atgggagacc tgagcgctct ttatctggtg attgaccacc agttttccga
     5401 tgccgcggtt ttcccaatcc gaggcgatca cgatgatgtg aaggtctttg gtgccggcgg
     5461 ccacgatcgc atcgatcagg ccttccggag ctcccacggc cagaaagcca ccgatggcaa
     5521 cccggctgtg gggtgctatc atggcagccg cttcagccgc tgtgatcagc ttgaacattg
     5581 attctccttt ttcccggaga gcataatctc cggtatatgt atattgatga ttgctattaa
     5641 tgggtgtggg acttgatcac tccacgatat acttttccac cagtctgagg tctcggccgc
     5701 tgaggctgag ctgcagccag atgccgtctt cacggtattc ctctgtgtga atatcggcaa
     5761 tctcatgcag gcgggaagcc agtccaccct gatcaaaggg cagatggagt gtgagctgcc
     5821 tggtatcgag caaagcctgt tccactctgc tcaacaggtc cgggatcctg tcctgctggc
     5881 tggcgctgat aaatactgcc cagggaaagc gccgcttcaa gagagtgaga taggtggcat
     5941 ccaggaggtc tatcttgttg agcaccagga tttgcggaag ctggtctgcc ccgatttcat
     6001 tcaggaccag attcacctgt tcgatatagt attcaaagcg ttcatccgag acgtccacca
     6061 tgtgcaggag cagatcggcg tcccgtactt ccatcagcgt ggcgcgaaac gatgccacca
     6121 gatgatgtgg cagccgggag atgaatccca ccgtgtctga gatcaccaaa ggctggccgg
     6181 tgctgagttt tagctggcgg ctggtggaat ccagagtggc aaagagcttg tcctccacca
     6241 gtactccagc cccagtcaaa gcgttgaaga gagtggattt accggcattg gtgtagccca
     6301 ccagacagag cttgagggtt ttatcccgtc ccttgcgctg ggtttccttt tgatgagtga
     6361 cggtctcgat ccagtgagtg accctgcgga tctgctgacg gatcagccgt ttatcgatct
     6421 cgatttgctt ttcgcccata cctctgctgg cggcaccacc cttggcgcgc atgctgccac
     6481 gttctttgtc aaagtggccc cagagcttgc gcagacgggg aagctggtat tccagctcgg
     6541 ccagtttgac ctgcagacgc gcttccctag tgcgggcatg gtcgtgaaag atgctgagga
     6601 tcacctctgt gcggtcgata accgtgatgc tgaattgctt ttcgatattg cggccctgga
     6661 tgggactcag ctcttcgttt acgatcagga gctcggcttg ggcctgatgc atcttctcag
     6721 cgatctcatt cagccagccc ctgccaaagt aagtggacct ttctggctgg ttgcgctttt
     6781 gcgtgtagcg tcccagcacc atgatccctg ctgtgtcggc aagcctttcc aactcatcaa
     6841 gactggcctc cacatccgcg gaagcctcat tctggcgcac gatcgcgact ataaatgctg
     6901 tcttttcatc agtttcaagc tcttcccagg attcgtcctc ctggtcaaac tcatcgagtt
     6961 caaaatctcg ttcttctatc atggctctcg ttgttaatta ggggttcagt ctggtcccgc
     7021 gggcatccat acggcccggt gagctgtcat agacctctac gttgtaatag atcacggcga
     7081 tgtaatcgcg cgggaaagca ccgtgaaaaa agctgtgcat gaggttcaca taacgggtga
     7141 ctttacgttc gtaatccgtg agagaggggt ccatgggatg cagaagccgg gcgatctgct
     7201 tgatcttgaa ggtggggatg tccaaaaaca cggtcacgca ggcgggattt accatctggt
     7261 tgataaaggt ctgggtattg tattggggct cggaatagag ctccagcgag accaacgcgt
     7321 ggggactgaa atgctcggaa gcgtgttgat agagggaatc cagcagatta agcttttgtt
     7381 ccatgccggc ttccatgtgg cttgacaaga ggtcaagcag ggcatccacg ctcttctcgt
     7441 ctggacagaa gcccatgccc ttgacggcgt tgttgagctt gaaagcgctg tctttgcggg
     7501 ccgcgccata agtggccacc atgccgttgt gaggaccgga aagctggggc tggcgcgtgg
     7561 cttggggtcc cttgctcaac atttcgcgaa agatttccag atactcagcc ctgcgagcgg
     7621 tgttccagct cataa
//
LOCUS       DAOSFN010000123         7608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_8329_length_7608_cov_8.999735, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000123 DAOSFN010000000
VERSION     DAOSFN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7608
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8329_length_7608_cov_8.999735"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            641..3199
                     /locus_tag="PLO35_07825"
     CDS             641..3199
                     /locus_tag="PLO35_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOH79617.1"
                     /translation="MKHALIVIFASALLVLSAAPFENLPTAVTQPDGSELQLLASGDE
                     YANRLHDASGYTVIQSPVDGYYYFASVVNGEPAPSAWRVGLHDPASLGLRTGINISPD
                     AYRAKVSFMNSHARSGHRGPNTGTVNNINVFIRFSDQDEFADSRAVYDAKFNTVGPGE
                     YSLRNYFHQVSYDQLNYVTHHYPVCAPEVNLSYQDSHPRAYYMPYNAVTNPQGYRDWQ
                     RTDREHALLADAIDAIASQVPADLNIDADNDDYVDNVCFIIRGPHSAWNDLLWAHRWA
                     LYTQDAYINGKMVWDFTFQPENQNSVRTLCHEMFHSVGAPDLYHYTFNGITPAGCWDI
                     MESGNGHMGMYMKYRYGGWIDNIPTIGAGTHTLQPITSSTGSVYKIPLSGSQYLVLEY
                     RKQGADVFEEYLPGSGLLIYRVNDDLDGNADGPPDEVYIFRPGGSSDANGDIAAAAFS
                     LNNRRTEFNTYTDPYCFLTGGNTSPVNIQNIGEAGDTITFTLLASTDAIPPVISNLLP
                     AQGSILTLADQMISAGVSAPGSVVTGVEFRWDGSLIGSDNTAPYSFNIPEALMTPGHH
                     DILVTAYNAETLFSTARSTVRVVDPSQASWFSWLTDEPIWESYGRGAVPIKAAVEFEL
                     GPQQYLVRKLAFNAVEDPWGNPAIPGRVNVKINRFGPSGITDETLLNIGDLDHPMNGR
                     FEYQLSSQTTISGRVAVIIDLMDYQNMVFDNNGPCGKSWLTEPNRPWTDALGRGIIGA
                     AAIELELIAPGSPNSDELLTPAITSVNPYPNPFNSKTCLAFELKNPARLKVSLFNLRG
                     QKVRDLFAGNLPGGTHQLEWDGLDSTGREVSNGLYFYRVDDGRRQFSGKLIRIK"
     gene            3357..3797
                     /locus_tag="PLO35_07830"
     CDS             3357..3797
                     /locus_tag="PLO35_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOH79618.1"
                     /translation="MIKDQEFELISPCGVYCGECACHKVKDNPALLPVLKAHGIDEEQ
                     LPCPGCRAVDGHCKHLGSRCENYACAQEHGVTMCYECEDFPCVRLMPAADRANSLPHN
                     MKMYSQCYINRYGVQAWLENYAEIRLRYFTGKISYGKGPQIEEK"
     gene            complement(3936..4340)
                     /locus_tag="PLO35_07835"
     CDS             complement(3936..4340)
                     /locus_tag="PLO35_07835"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOH79619.1"
                     /translation="MIFEYRKRIYGYECDIYGHLNNANYLQLLEAARAEALIEMEMSI
                     ARMRELDLQIFVTAFELRYIKAIQLEDIVTVKTWATSITRLRGHWRQEVYDGTGTLCF
                     TAEMDAVFARGGKPQRLSAEVLEVFVSRTETQ"
     gene            4499..>5049
                     /locus_tag="PLO35_07840"
     CDS             4499..>5049
                     /locus_tag="PLO35_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79620.1"
                     /translation="MSRKTVNPKYAKPGSKVGHAAETALHTSPDWTRHLPWVLVAVLF
                     LMLSLIYFPVAYGGKTPIASDITQWQGAAKSIIDYNKEHSDQALWTQSMFSGMPAYMI
                     SFPNRYPFLENISRLTDKVINWRIFLLFIGGLGIFLLLRFWKMNPWICFAGAVAFVFS
                     CHWVGLLEIGHNTKFRAIMYVPWV"
     assembly_gap    5050..5149
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5150..>6423
                     /locus_tag="PLO35_07845"
     CDS             <5150..>6423
                     /locus_tag="PLO35_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79621.1"
                     /translation="YWGFMPFTQIYNYFGIVVLAFGILAVLGKKHRRLAIFMWITSLI
                     FTLMSFGSFAPALSDLLLKYLPYFNKFRVPSMILTMVQINAVMLAALGLDTLFDRAEA
                     QDKSFGKLLLRLFWIGGGLFILWLALSKTLLGGLSFSNAGELAQYKQRGYDQVPAELI
                     ASRLDLLYKSGVLSLLLLTVSLGFAYLRAIGKLKNTAFMILITLTIFVDLWVYTGKHM
                     RDLKPVEQYLNRFEAADYDQFMLRDKTPHRIYPISQSLFTQSSLPKPSGEWAYHHQIV
                     TGYSAAKLKRYDDLLKLLDGNPAKQRPGEWQRYLMGLFAVEDGQQPREIPMPVLSMLN
                     AKYFVHPEAIPMDSLLTQIRPVFTSEDGITIYENRNVLPRAWFVDEVRQVSPADSILE
                     LMSAESFDPRKTAYVESPVQGVTKPDSTMVKQT"
     assembly_gap    6424..6523
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6808..7365
                     /locus_tag="PLO35_07850"
     CDS             6808..7365
                     /locus_tag="PLO35_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79622.1"
                     /translation="MWSLDSRSYRSALTAPRRKFYASEPGYLKWPLLFFFIAAIASQG
                     LFAQGNSEEFVALKRYTLPSTAIPIQDLVEKDANFSLRGQPYSGIAFERYENGQLLRV
                     VHLHQGLQNGPLYLWYPNGNPQMSANYRMGRLRGRFLGWYANGGTIYDMMIGTNGYNG
                     DMLEDDSQRGDDDIDDTEMEGTDND"
     gene            7400..>7608
                     /locus_tag="PLO35_07855"
     CDS             7400..>7608
                     /locus_tag="PLO35_07855"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOH79623.1"
                     /translation="MKVEGRRIVMVTAYDYAMGKIAAQSGIDLILVGDSLGMVVLGYD
                     TTLQVTMADMLHHSAATRRGAPQSF"
BASE COUNT         1704 a         2031 c         1895 g         1778 t
ORIGIN      
        1 gggttacgcg tgctggtcta tctgaaatat cgcccctgac tgggctttgg acttacgtgc
       61 agcacatcga agacatttat ctcgcgggtg tactggttga agtccaagcg gcccgcaatc
      121 tcgctgtcat gtatgccttt atcgtggccg cttgaactcc aacggggctt atgttagcct
      181 cttgtttagt aagttttcgc acaagctcgg aggcgggagt ccaacttact gacaactctt
      241 tgctacacaa agggctggat actcccctcc gcgagtatga caaggtgctg acacgtgtgc
      301 caaggctgct tttcatggtt acccacgctt ccctgtcagc cactcacgct tatcccgtgc
      361 ttatcgcacg cctatcccgt gcttcagcat gcgtgagggg tgggatacga gaaccataag
      421 cggaagagag tggcaagcat gatgtttctg gccttcaagg catgatcatg gattctggat
      481 atctgcttcc atcacaagca ataacgctct ctgacaaaag ggttccgggc tccttaccca
      541 tcttgattat cgcctttgtc cagtccggga cgaggaaagt aatgaactaa ttatgcaaca
      601 aggcacgaga tttgctttac atgacaaagt tggaggaatg atgaaacacg ccttgattgt
      661 gatctttgca tcagcgctgt tagtgctgag cgcggccccg tttgaaaacc tgcccactgc
      721 cgtcacccaa ccggatggaa gcgaactgca actgctggct tcgggtgatg aatatgccaa
      781 ccgtctgcac gacgcgtcag gctacactgt catccagagt cccgtggacg gatactatta
      841 ctttgcctct gttgtgaatg gagagcctgc tccatcggcc tggagagtgg gtctgcatga
      901 tcctgccagc ctgggactga ggaccgggat caacatttct cctgatgctt acagggccaa
      961 agtgagcttt atgaactccc acgccagaag tggtcataga ggtcctaaca ccggtactgt
     1021 caacaatatc aacgtcttca tccgcttcag cgaccaggat gaatttgccg atagcagagc
     1081 ggtttatgac gccaaattca ataccgtggg gcctggtgaa tactccctgc gcaattactt
     1141 tcaccaggtg agctacgatc agctcaacta tgtgacccat cactaccccg tctgcgctcc
     1201 cgaggtcaac ctctcctatc aggacagcca tccaagggct tattatatgc cctacaacgc
     1261 ggtaaccaat cctcaaggct accgggactg gcaacgcacc gaccgcgaac acgcgctcct
     1321 cgcggatgcc atcgatgcca tcgcttcgca ggtgccggct gacctcaata tcgacgctga
     1381 taacgatgat tatgtggata acgtctgctt catcatccgt ggtcctcaca gcgcctggaa
     1441 tgatctcttg tgggcccatc gctgggcgct ctatacccag gatgcctaca tcaacggtaa
     1501 gatggtgtgg gacttcacct tccagcctga aaaccagaac agcgtgcgca ccctctgcca
     1561 tgagatgttt cacagtgtgg gcgcgccaga cctctatcac tataccttca acggcatcac
     1621 tccagcggga tgctgggaca tcatggaaag cggcaacggc catatgggca tgtatatgaa
     1681 gtatcgctat ggcggctgga tagacaacat cccgacaatc ggagctggga ctcatactct
     1741 tcagcccata acctcatcca cgggaagcgt ttacaagatt ccgctgtccg gcagccagta
     1801 tctggtattg gaataccgca aacaaggcgc ggatgtcttt gaagaatatc tgccgggttc
     1861 gggactactg atctatcgcg tcaatgacga cctggatggc aacgcggacg gccctccgga
     1921 cgaagtgtat atcttccgtc cgggtggaag ttcagacgcc aatggcgata tcgccgccgc
     1981 agcttttagc ctcaataacc gccgcaccga gttcaatacc tacaccgatc cctattgctt
     2041 tctcaccggt ggcaatacgt ctcctgtcaa tatccaaaac attggcgaag cgggtgatac
     2101 catcactttc acgcttctcg cgtccaccga tgccattcct cccgtgatct ccaatctctt
     2161 gccagcccag ggcagcattc tcacgctggc agaccaaatg atcagcgccg gagtcagcgc
     2221 tccgggctcg gttgtgaccg gtgtggagtt cagatgggac ggcagcctga tcggtagtga
     2281 caacacggcc ccctactcct tcaatatccc ggaagccctg atgacacctg gtcatcacga
     2341 cattctggtg actgcctaca acgcggaaac gctgttcagc acagcccgct ccactgtgag
     2401 agtggtggat ccttctcagg cctcctggtt tagctggctc accgatgagc caatctggga
     2461 aagctatggc cggggagccg tgcccatcaa ggccgcggtg gaatttgagc tgggccccca
     2521 gcaatacctg gtacgcaagc tggctttcaa cgcggtggaa gatccctggg gcaatcctgc
     2581 catcccggga agggtgaatg tgaagatcaa ccgcttcggt cccagtggga taacggacga
     2641 gaccctgctc aatatcggag acctggacca ccccatgaac ggccgctttg aatatcagct
     2701 ctcaagtcaa accaccatct caggcagggt tgccgtgatt atcgacctca tggattatca
     2761 gaacatggtc ttcgacaaca atggcccctg tggaaaaagc tggcttacag agcccaaccg
     2821 tccctggaca gatgctctgg gacgtgggat tatcggggcc gcggccatcg aattggaact
     2881 tatagctccc ggctctccca attccgatga actgctcact cccgcgatca cttcagtaaa
     2941 tccatatccc aatcccttta acagcaagac ttgtctggct ttcgagctta agaatcccgc
     3001 ccggttgaaa gtctcccttt tcaacctgag aggtcagaaa gtgcgagacc tgtttgcggg
     3061 aaacctccct ggcggtactc atcaattgga atgggatggc ctggattcaa ccggtcggga
     3121 ggtctccaac ggcctttact tttacagagt ggatgatggc agacgccagt tcagcggcaa
     3181 gctgatcagg ataaaatagc caggggaata tccaatttga cataggtagc ccggtcaggt
     3241 tgatcagaat agcgctgagc tcaattattt agcgctactc ctgtggtcct ggtcactttt
     3301 tgcttgacga aatcgctctg agctcatcct gggagcaaaa tcccaagagg tgtgatatga
     3361 tcaaggatca ggagtttgaa ctgatctctc cctgcggtgt ctattgtggt gagtgcgcct
     3421 gccataaagt gaaggacaat cccgctcttc tgcctgtttt gaaagcccac ggcatcgatg
     3481 aggagcagct gccctgccca ggctgccggg cagtggatgg ccactgtaaa catctgggct
     3541 ctcgctgcga aaactatgcc tgcgcgcagg agcatggagt gacgatgtgc tatgaatgcg
     3601 aggactttcc atgcgtgcgt ctgatgcctg ccgcggaccg ggctaactct ttgcctcaca
     3661 atatgaagat gtactctcag tgctatatca atcgctacgg cgttcaggcc tggctggaga
     3721 attatgcaga gatcagacta cgttacttta ccggtaagat cagctatggg aaggggcctc
     3781 agatagagga gaaataagga cagaagtgag gcttatctat catggacaag ctcctatttg
     3841 tccctgacat tatgcttcat aaacattatg tttgacaagc ataatgttgc ctaacaatct
     3901 cattatgcca tgcaaaaata gcatgttacg tctctttatt gagtctcagt tctgctgaca
     3961 aagacctcca atacctcagc cgaaagcctc tgaggcttcc ctccccttgc aaacacggcg
     4021 tccatctcag cagtgaagca aagtgttccg gtaccgtcat atacctcttg acgccaatgc
     4081 cccctgaggc gtgtgatgct ggtggcccag gtctttacgg tcacgatatc ttccagctgg
     4141 atggctttga tatacctgag ctcgaaagcg gtgacaaaga tctggaggtc cagctctctc
     4201 attctggcga tcgacatctc catttcgatc agggcttcgg cccgggcggc ttctaaaagc
     4261 tgcagatagt tggcattgtt caaatggcca tagatgtcgc attcatagcc atatatccgt
     4321 ttgcggtatt caaatatcat cgctgactcc ttgggctcga gtaatcgtct gttccagtca
     4381 ttgagagctg taaggctggt tggcaggccg gccacgcttt gcgcagcgat tttatccttg
     4441 accgataagg gcggacgatt tctcatgcga taaaatacgt tttggataga ggatttatat
     4501 gtccaggaaa actgtcaatc ccaaatatgc caagcccggc agcaaagttg gacacgcggc
     4561 tgaaacggca ttacacacga gcccggactg gaccaggcat ctgccctggg tattggtggc
     4621 cgtgctgttt ctgatgctgt ctctgatcta ttttcccgtg gcctatggcg gcaagactcc
     4681 catcgcctcc gatatcactc aatggcaggg tgccgccaag agcatcattg actacaataa
     4741 agagcattcc gatcaagcgc tgtggactca gagcatgttt tccggcatgc ctgcctacat
     4801 gatctccttc ccaaaccgct atcccttttt ggaaaacatc agccgcctca ccgataaggt
     4861 gatcaactgg cggattttcc tgctctttat aggtggactg ggcattttcc tgctgctgag
     4921 attctggaag atgaatccct ggatctgttt tgccggagcg gtggcttttg tgttttcctg
     4981 ccattgggtg ggcctcttgg agataggcca caataccaag ttccgggcta tcatgtatgt
     5041 cccctgggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt attggggctt
     5161 tatgcccttc acccagattt acaactactt tgggatcgtg gtgctggcct ttggcatcct
     5221 ggccgtgctg ggcaaaaagc atcgccgcct ggccatcttt atgtggatca cgtcactcat
     5281 ctttaccctg atgtcctttg gcagcttcgc tcccgccctg tctgatctgc tgctcaaata
     5341 tctgccctat ttcaacaaat tccgggtgcc ttccatgatc ctcaccatgg tgcagatcaa
     5401 tgccgtgatg ctggccgccc tgggtctgga caccctcttt gacagagcgg aagcccagga
     5461 caagagcttt ggcaaactgc tgctgaggct gttctggatc ggtggaggtc tcttcattct
     5521 gtggctggca ctctccaaaa ctctcttggg aggcttgtct tttagcaacg cgggagaact
     5581 ggcccagtat aaacaacgcg gctatgatca ggtcccggct gaattgatcg cttccaggct
     5641 ggacctcctt tacaagagcg gagtgctgag cttgttgctc ctcaccgtga gcctgggttt
     5701 tgcctatctg agagcgatcg gcaaactgaa aaacaccgcc ttcatgatcc tgatcacgct
     5761 gactatcttt gtagacctct gggtctatac cggaaagcat atgcgcgacc tcaaaccggt
     5821 ggagcaatat cttaaccgct tcgaggcggc cgattatgac cagtttatgc tgcgcgacaa
     5881 gacccctcac cggatctatc ctatctccca gagccttttc actcagagtt cactgcccaa
     5941 gccaagcggc gagtgggcat atcatcatca gatcgtgacg ggttattcag ccgccaaact
     6001 gaagcgctat gacgatctgc tcaagctgtt ggacggcaat cccgccaaac agcgtcccgg
     6061 cgaatggcaa agatatctga tgggcctatt cgcggtcgag gatggccagc agcccagaga
     6121 gatacccatg ccggtgctca gtatgctgaa tgccaaatac tttgtccatc cggaagcgat
     6181 tccgatggac agcctcctca cccagatcag gccagtcttc acgtcagagg atgggatcac
     6241 catctatgaa aaccgcaacg tgctgcccag ggcctggttt gtagatgaag tccggcaggt
     6301 cagcccggct gattctatcc tggaactgat gagcgccgag agctttgatc cccgcaaaac
     6361 cgcctatgtg gagagccctg tccagggagt taccaagcct gattccacca tggtaaaaca
     6421 gacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcggtc tgctggcatc
     6541 catcctggcc ttagtggcag gactggggct tccctacctg aaaaaaccgg ctaaacccat
     6601 tatctgacct taatatgaga ccgggcaggg atcccgcgcc cggtcttatc tttttgggtt
     6661 gttctcactg ctgattataa tgtattatga gagttatgca cattgctggt ttgaataccc
     6721 cctgtcatgt ctctcaaacc agtcaatagg ataaacaaac cggggacagc tgaaaaaagc
     6781 agttgacgtc cccaagggag tttgaaaatg tggtcattag attccagatc atatcggagc
     6841 gctcttaccg ctcccaggag aaagttttac gcatcagagc ctggctacct gaagtggcct
     6901 ctgcttttct tcttcatcgc cgcgatcgca tcccaggggc tttttgccca aggcaattcc
     6961 gaagaattcg tggccctcaa gcgctacacc ctgccctcaa ccgccattcc cattcaggac
     7021 ctggtggaga aggacgccaa tttcagcctg agaggccaac cttattccgg aatcgctttc
     7081 gagcgctatg aaaatggcca gctcctgagg gtggtccacc tccatcaagg cctgcagaac
     7141 ggtcctctct atctttggta tcccaacggg aatccccaga tgagcgccaa ttacaggatg
     7201 ggacgtctgc ggggccgctt tttgggatgg tatgccaatg gcgggaccat ctacgatatg
     7261 atgatcggca ccaatggcta caacggtgat atgctggaag atgattccca gcgcggcgat
     7321 gacgatatag atgacacgga aatggaagga accgataatg actgacaaag agcagctgag
     7381 cgtaaccagc tttcaacaga tgaaagtgga aggacgccgg atcgtgatgg tcacggctta
     7441 cgactatgcc atgggcaaaa tcgccgccca aagcgggatc gatctgatcc tggtgggtga
     7501 cagcctcggc atggtggtcc tgggttacga cacaactctg caagtgacta tggcagatat
     7561 gcttcaccat agtgctgcca cccgcagagg cgctcctcaa agctttat
//
LOCUS       DAOSFN010000124         7456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_8560_length_7456_cov_9.493852, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000124 DAOSFN010000000
VERSION     DAOSFN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8560_length_7456_cov_9.493852"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..270)
                     /locus_tag="PLO35_07860"
     CDS             complement(85..270)
                     /locus_tag="PLO35_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOH79624.1"
                     /translation="MAKKSLILKQQREPKFKVRKYNRCLICGRPRAFMRDFGMCRLCF
                     RKYASLGHIPGVTRSSW"
     gene            complement(280..825)
                     /gene="rplE"
                     /locus_tag="PLO35_07865"
     CDS             complement(280..825)
                     /gene="rplE"
                     /locus_tag="PLO35_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOH79625.1"
                     /translation="MTRLKEQYKSQVIPAMQKRFAYKNPHQVPRLVKIIVSMGVGSAT
                     QNKAILDNAVKDMEQITGRKAIVTKARKSISNFKLRQGMPIGCKVTLRDEVMYEFYDR
                     LTSIVIPRIRDFRGIPADAFDGRGNFSMGLKEQTVFPEIEYDKVDAIRGLNISIVTTA
                     RTDEECRELLKELGMPFQRNE"
     gene            complement(838..1143)
                     /gene="rplX"
                     /locus_tag="PLO35_07870"
     CDS             complement(838..1143)
                     /gene="rplX"
                     /locus_tag="PLO35_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOH79626.1"
                     /translation="MKLKKGDLVIVISGDDKGKKGHVLKAFPKTHRVIVEKVNLIKKH
                     AKPSQRNPQGGIITKEAPVHASNVMLFNEKLNAVSKPVVQMREGVRVRVCKKSGDEL"
     gene            complement(1165..1533)
                     /gene="rplN"
                     /locus_tag="PLO35_07875"
     CDS             complement(1165..1533)
                     /gene="rplN"
                     /locus_tag="PLO35_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOH79627.1"
                     /translation="MIQAQTVLNIADNSGAKKAMCIKVLGGTRRKYASLGDVIVVAIK
                     SATPGGKVKKGTVERAVIVRTAKEVRRPDGSYIRFADNAAVIIDDKHEPKGTRIFGPV
                     ARELREAHYMKIVSLAPEVL"
     gene            complement(1545..1799)
                     /gene="rpsQ"
                     /locus_tag="PLO35_07880"
     CDS             complement(1545..1799)
                     /gene="rpsQ"
                     /locus_tag="PLO35_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOH79628.1"
                     /translation="MALNRKMIKQGIVVSDANDKTIVVRVLRQYIHPLYKKTVRRHKK
                     FMAHDELNEAHVGDTVQIMESRPMSARKRWTLHKIVDRSK"
     gene            complement(1803..2003)
                     /gene="rpmC"
                     /locus_tag="PLO35_07885"
     CDS             complement(1803..2003)
                     /gene="rpmC"
                     /locus_tag="PLO35_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOH79629.1"
                     /translation="MKIEELRELHEGELQHRLEELRIELFNLRFQKAKNLLDRPDRLR
                     IVRREIARINTIVKENEQKAVR"
     gene            complement(2000..2419)
                     /gene="rplP"
                     /locus_tag="PLO35_07890"
     CDS             complement(2000..2419)
                     /gene="rplP"
                     /locus_tag="PLO35_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOH79630.1"
                     /translation="MLAPKKIRHRKMMKGRRTGLSWTGSNVDFGDYGLIALEDAFITS
                     RQIEAARIAITRHMKRLGKVWIRIFPDKPITKKPAETRMGKGKGAPEYWVAVVRPGRV
                     LFELEGVDVKIAKEAMRLAAHKLPIKTRLIAREGVEL"
     gene            complement(2430..3077)
                     /gene="rpsC"
                     /locus_tag="PLO35_07895"
     CDS             complement(2430..3077)
                     /gene="rpsC"
                     /locus_tag="PLO35_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOH79631.1"
                     /translation="MGQKIHPILYRLGVNKDTESIWFASGSSYVDFLQEDIKIRTYIQ
                     KRLAQKMVSRVQISRKTSSITIDIHTARPGLVIGKKGEDIDRLRGELNVLINKNRPNP
                     IAVMINIEQIDKLWLDARLAGLEISRQLEERVSFRRAMKMAMRNVMKEGALGVKVQVS
                     GRLGGAEIARTERYKQGRTPLHTLRADIDYAIVEANTTYGVIGIKVWIYKGDILS"
     gene            complement(3088..3426)
                     /gene="rplV"
                     /locus_tag="PLO35_07900"
     CDS             complement(3088..3426)
                     /gene="rplV"
                     /locus_tag="PLO35_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOH79632.1"
                     /translation="MEATAKLSFVRGSARKARLVLDTIRYKKVTEAQNLLKFSNRRSA
                     AQIHKLLESAIANARVKEPKVDIAQMYVTKATADEGPQMKRFMPRAQGRAFMIRKATC
                     HIALEIQTIE"
     gene            complement(3437..3712)
                     /gene="rpsS"
                     /locus_tag="PLO35_07905"
     CDS             complement(3437..3712)
                     /gene="rpsS"
                     /locus_tag="PLO35_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOH79633.1"
                     /translation="MARSIKKGPFVDGHLEKKVDVLNTDSKKNVIKTWSRRSVILPSF
                     IGHTFSVHNGHKFVPVYVTENMVGHKLGEFSPTRTYRGHKDKKKKGR"
     gene            complement(3724..4554)
                     /gene="rplB"
                     /locus_tag="PLO35_07910"
     CDS             complement(3724..4554)
                     /gene="rplB"
                     /locus_tag="PLO35_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOH79634.1"
                     /translation="MGVKKYKPTTPTLRFRTGYTFEEITCDSPEKSLLKPVRKSGGRN
                     NQGRITCRHRGGGHRRHYRVIDFKRDKIGIPARVATIEYDPNRTARIALLHYVDGEKR
                     YIIAPDGLTVGSKVMSGPEAEIAVGNAIPLERIPLGSTVHNIELKRGRGGQIARSAGT
                     YGQVVAKDGDYVHVKMPSNDVHLIRKECLATMGTVSNSDHNLIQLGKAGRKRWLGIRP
                     TVRGVAMNPVDHPMGGGEGKSSGGGHPVSPWGKPAKGGKTRKTRKYSDKYIVKAVKKR
                     "
     gene            complement(4567..4854)
                     /gene="rplW"
                     /locus_tag="PLO35_07915"
     CDS             complement(4567..4854)
                     /gene="rplW"
                     /locus_tag="PLO35_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOH79635.1"
                     /translation="MIHPRNIVIAPIITEKSGIQMHATNTYTFKVSMNANKIEIKKAI
                     EHIFSVKVLNVNTIRMMGKPKRLGRYNGKRPDWKKAIVTLREGDKIADFEA"
     gene            complement(4851..5486)
                     /gene="rplD"
                     /locus_tag="PLO35_07920"
     CDS             complement(4851..5486)
                     /gene="rplD"
                     /locus_tag="PLO35_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOH79636.1"
                     /translation="MVKAIKFNSQGVQIGEVELPVSVFDVDVNSPKVLLHEVVTMYLG
                     NQRQGTVQKKNRAMTAGSTRKLFKQKGTGNARQGSRRTPVRVHGGRAFAILPKDWYRP
                     IPRTKKRMALKVALTDRAREGRVFIVESFDFNTPNTKSAMELLAKIAPEKGRKLVVTD
                     GHHLPTVKSFTNIPDVMTDRADSLHAYEILKSSYVVLTQDALNKVEEVFSS"
     gene            complement(5501..6133)
                     /gene="rplC"
                     /locus_tag="PLO35_07925"
     CDS             complement(5501..6133)
                     /gene="rplC"
                     /locus_tag="PLO35_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOH79637.1"
                     /translation="MLGLIGKKIGMTQVFDSNGKVVPVTVIKAGPCRVVSKRSADAHG
                     YDALQLGFEEIKEKNCTRPLIGHFKKNGSPIYRYVREFRPSFGQETAEFEVGAELKAD
                     LFNESETVTVTSKSKGRGYAGVMKRHNFAGFEQSHGVHESFRGPGSIGQCAQPSRVFK
                     GVKMAGHMGNERVSVRHLVVVKVDVEQGLVMVKGAVPGHRNSLVLIQKEQ"
     gene            complement(6136..6462)
                     /gene="rpsJ"
                     /locus_tag="PLO35_07930"
     CDS             complement(6136..6462)
                     /gene="rpsJ"
                     /locus_tag="PLO35_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOH79638.1"
                     /translation="MSKLDNRIRIKLKAYDHRLLDQSVAEIVKSTRGTGAKVIGPIPL
                     PTDRSVYTILRSPHADKKSQDQFQMLVHKRLIDIMNPTQQTTNALKKLSLPAGVHVEI
                     KANTRS"
BASE COUNT         1430 a         1963 c         2052 g         2011 t
ORIGIN      
        1 tgtccggcac gatatgcatt gcggatcttg gtcaatgcat cagctatcgg atcggttacg
       61 ctcatttgtt tcctccacta tgggttacca gctggatctc gtcacaccgg gaatgtggcc
      121 cagggacgcg tatttgcgga agcagagacg gcacatgccg aagtctctca taaaggcacg
      181 tggccttccg cagataaggc agcggttgta ttttcttact ttgaacttag gttcacgttg
      241 ttgtttgagg atcagagatt tcttggccac ttagcctcct tattcgttgc gctggaacgg
      301 catgcccagc tctttgagca gctcgcggca ctcttcgtcc gtgcgggctg tggtcacgat
      361 tgagatatta aggccgcgga tggcgtccac tttatcatat tcgatctctg ggaacacggt
      421 ctgttccttg aggcccatgc taaagttgcc gcgtccgtca aaggcgtcgg ccgggatacc
      481 acgaaagtcg cggatgcggg gaatgacgat ggaggtgagg cgatcgtaga attcatacat
      541 cacttcatcg cgcagggtca ctttgcagcc gatcggcatg ccctggcgca gcttgaagtt
      601 tgatatgctc ttgcgggcct tggtcacgat cgctttgcgg ccagtgatct gttccatgtc
      661 tttcacggcg ttgtccagga tggccttgtt ctgcgtggcg ctgcccactc ccatgctgac
      721 gatgatcttc accagccggg gcacctgatg cgggttcttg taggcaaagc gtttttgcat
      781 agccggaatc acctggctct tatattgttc tttcaaacgc gtcatttcat tgctccttta
      841 cagctcgtcg ccagattttt tgcagacacg gacgcgaacg ccctcacgca tctgcaccac
      901 tggtttggac acggcgttca gcttctcgtt gaagagcatc acgttggagg catggactgg
      961 agcctctttg gtgataatcc caccctgggg gttgcgctgt gaaggcttgg cgtgtttttt
     1021 gatcaggttc actttttcca cgatgacgcg gtgggtcttg ggaaaagcct tgagcacatg
     1081 gcctttcttg cccttgtcgt ctccggagat cacgatcacc agatcgcctt tcttgagttt
     1141 caatctgtct gacatttcat ctccctacag cacttccggc gccagagaca cgatcttcat
     1201 gtagtgagct tcgcggagtt cgcgagctac cggaccaaag atgcgggtgc ccttgggctc
     1261 gtgcttgtcg tcgattatca cggcggcgtt atcggcaaaa cggatatagg aaccgtccgg
     1321 acggcgcact tctttggcgg tgcgtacgat cacggctcgt tccactgttc cttttttgac
     1381 tttgccgccc ggtgtggcgg atttgatcgc gacaactatg acgtcaccca gagaggcgta
     1441 tttacgtctg gtgccaccca ggaccttgat acacatggcc tttttggcac cggagttatc
     1501 ggcgatattc aatacggttt gtgcttgaat cattccaaaa ccccttactt gcttctatcc
     1561 acaatcttgt ggagagtcca gcgtttacgg gcactcatgg gacgcgattc catgatctga
     1621 acggtatcgc cgacatgagc ttcgttgagt tcgtcgtggg ccataaattt cttgtggcgg
     1681 cgcacggtct tcttatagag tggatggata tactgcctca ggacacgcac tacgatggtc
     1741 ttgtcgttgg cgtcgctgac gactatcccc tgtttgatca ttttacgatt gagagccacg
     1801 atttacctca ctgccttttg ctcattttct tttacgatgg tgttgatgcg ggcgatttcc
     1861 cttctcacga tgcgcagccg gtcagggcgg tccaggaggt ttttggcttt ctggaagcgc
     1921 aggttaaaga gctcaatccg cagttcttcg agccggtgtt gcagctctcc ctcatgcagt
     1981 tcgcgcagtt cttcgatctt cataattcaa ctccttcgcg ggctatcagc ctggtcttga
     2041 tggggagttt gtgagccgcg aggcgcatgg cttctttggc tatctttacg tccacgcctt
     2101 ccagctcaaa gagcacccgg ccgggcctca ccacggctac ccagtattcc ggagcgcctt
     2161 tgccttttcc catgcgggtt tcggctggtt tcttggtgat gggcttatcc gggaaaatgc
     2221 ggatccacac cttacccaag cgcttcatat ggcgggtgat agcgatacgg gcggcctcaa
     2281 tctggcgact ggtgatgaag gcatcctcga gagcgatcag gccataatcg ccaaaatcga
     2341 cgttggatcc ggtccaggag aggccggtgc gtctgccttt catcatcttg cgatgtctta
     2401 tcttttttgg tgctaacatc ttatctctcc tagctcagga tgtcgccttt gtagatccac
     2461 accttgatgc cgatcacgcc ataagtggta ttggcttcca cgatggcata gtcgatgtcg
     2521 gcacgcaggg tatgaagcgg ggtgcgaccc tgtttgtagc gctcggtcct cgcgatctcg
     2581 gctccgccaa gacgtccgga aacctgcact ttgacgccca gggcgccttc tttcatcacg
     2641 ttgcgcatgg ccattttcat ggcgcggcgg aaagagacgc gctcttccag ctgacgcgag
     2701 atctcgaggc ccgccagacg ggcatccagc cacagcttgt cgatctgctc gatgttgatc
     2761 atcacggcga tgggattagg ccggtttttg ttgatcagca cgttgagctc gcctctgagg
     2821 cgatcgatgt cctcaccctt tttaccgatc acaagaccgg gccgggcagt gtggatatcg
     2881 atggtgatgg agctggtctt gcgggaaatc tgcacgcggg agaccatctt ctgggccaaa
     2941 cgcttttgga tgtatgtgcg tatctttata tcttcctgca ggaaatccac ataggaagac
     3001 ccactggcaa accagatgga ttcggtgtct ttattgacgc cgaggcgata caggatgggg
     3061 tgtattttct gtcccaagtt cttcctccta ttctatggtc tggatttcca gggcgatgtg
     3121 acaggtcgcc ttgcggatca tgaaggccct gccctgggct ctgggcataa agcgcttcat
     3181 ctgagggcct tcgtctgccg tggctttggt gacatacatc tgagcgatgt ccaccttggg
     3241 ttctttgacc cgggcattgg cgatggccga ttccagcagt ttatggatct gagcggcaga
     3301 cctgcggttt gaaaacttca ggaggttttg ggcttcggtc accttcttgt agcggatggt
     3361 gtcaagcacc agacgggcct tacgggccga tccacgtacg aagctgagtt ttgctgttgc
     3421 ttccattgta acttccttat ctgccttttt tcttcttatc tttgtgaccg cggtaggtgc
     3481 gcgtgggtga aaactcgccc agcttatggc ccaccatgtt ctccgtgaca tacaccggca
     3541 caaatttgtg gccgttgtgc acagagaagg tgtgaccgat aaaggagggc aggatcacag
     3601 aacggcgcga ccaggtcttg atcacgttct tcttgctgtc ggtattgagc acgtccacct
     3661 tcttctcgag gtgtccgtcc acaaagggtc ctttcttaat cgagcgtgcc attatctact
     3721 cccttatctc tttttgaccg ctttcacgat gtacttatcg ctgtatttgc gggttttacg
     3781 ggtcttgcca cccttggctg gcttacccca gggagagacg ggatgtccgc caccggagct
     3841 tttaccttcg ccaccgccca tggggtgatc gacagggttc atcgccacgc cgcgaacggt
     3901 gggacggata cccagccaac gtttgcggcc ggctttgcca agctggatca ggttgtgatc
     3961 ggagttgctg acggtgccca tggtggccag gcattccttg cggatcagat gcacgtcgtt
     4021 ggagggcatt ttcacatgca catagtcacc gtccttggcc actacctggc cataggtgcc
     4081 ggcgctgcgg gcaatctggc ctccacggcc acgcttgagc tcgatattat gaacggtgct
     4141 gcccagagga atcctctcca gcggaatggc attgcctacc gctatctcag cttcgggacc
     4201 gctcatcact ttgctgccca cggtcaggcc gtcgggagcg atgatataac gcttttcgcc
     4261 gtccacatag tgcaggagcg cgatgcgggc agtgcggtta ggatcgtatt cgatcgtggc
     4321 gacgcgagcc ggaatgccga tcttgtcgcg tttgaaatcg atcacgcggt agtggcgacg
     4381 gtgtccgcct ccacgatgac gacaggtgat cctgccctgg ttgttgcggc cgccgctttt
     4441 gcggacgggc ttgagcaggg atttttctgg gctgtcgcag gtgatctctt caaaggtata
     4501 accggtgcga aagcgcaggg taggggtggt gggtttgtat tttttaactc ccatgttata
     4561 cctcttttat gcttcgaaat cggcgatctt gtcgccttcg cgaagggtta ctatggcttt
     4621 tttccagtcg gggcgcttgc cgttgtagcg gcccagacgt tttggcttgc ccatcatgcg
     4681 gatcgtgttt acgttcaaca ctttcacgct gaagatgtgc tcgatcgcct tcttgatctc
     4741 gatcttgttg gcgttcatgc tcaccttgaa ggtgtaggtg ttggtggcgt gcatctggat
     4801 cccgctcttt tcagtgatga tgggggcgat gacgatattg cgcggatgga tcatgagctg
     4861 aatacctcct cgactttgtt gagagcgtcc tgagtgagca ccacgtagct gctcttgagg
     4921 atctcatagg cgtgcagact gtcggcacgg tcggtcatca catccgggat attggtgaag
     4981 cttttcaccg tgggaagatg atggccgtcg gtgaccacaa gcttgcgtcc cttctcggga
     5041 gcaatcttgg ccagcagttc catagcgctt ttggtgttcg gagtattgaa gtcaaaagac
     5101 tccacgataa agacgcggcc ttcacgggca cggtctgtga gggcgacctt gagggccatg
     5161 cgtttcttgg tgcgcgggat gggccggtac caatccttgg gcaggatggc gaaggcgcgg
     5221 ccaccatgaa cgcggaccgg agtacgacgg gaaccctgac gggcgtttcc ggtacctttc
     5281 tgtttgaaca gcttgcgggt gctgccagcc gtcatggcac ggttcttttt ctggacggtg
     5341 ccctgacgct ggttgcccag atacatggtc accacttcat ggagcagtac tttgggggag
     5401 ttgacatcca cgtcaaagac gctcacagga agctcgacct cgccgatctg aacgccctgg
     5461 ctgttgaatt ttatagcttt taccatttgt ttctctcccc ttattgctct ttctggatca
     5521 ataccaggga attacggtgg ccgggtacgg cacctttgac cattacaagg ccttgctcca
     5581 catccacttt cactaccacg agatggcgca cggatactct ctcattgccc atgtggccag
     5641 ccatcttcac gcctttaaac acgcgtgagg gctgagcgca ctgaccgatg gaaccaggcc
     5701 cgcggaagga ttcatgcaca ccgtggctct gctcaaaacc ggcaaagtta tggcgcttca
     5761 tcacaccggc atagccgcgg cccttggatt tggaggtcac ggtcacggtc tcggattcgt
     5821 taaacagatc ggctttaagc tcagcgccca cttcaaactc ggcggtctcc tgtccgaaag
     5881 acggacggaa ctcgcgcacg taacgatata tagggctgcc gttcttttta aaatgtccga
     5941 tcaggggacg ggtgcaattc ttttctttga tctcttcaaa acccagctgc agagcgtcat
     6001 agccatgggc atcggcactg cgtttgctga ccacgcgaca gggtcccgct ttgataacgg
     6061 taacgggcac gaccttgccg ttggagtcaa acacctgggt cattccgatt ttctttccaa
     6121 tcaatcccaa catgtctagc tcctggtatt ggctttgatc tccacatgca cgccagcggg
     6181 cagactcagc tttttgaggg cattggtggt ctgctgggtg gggttcatga tgtcgatcag
     6241 tctcttgtgg accagcattt gaaactggtc ttgcgatttc ttgtcggcat gaggtgaacg
     6301 caggatcgtg tacaccgatc tgtctgtggg aagtggaatg gggccaatga ctttggcacc
     6361 cgtaccgcgg gtgcttttca ctatttccgc cacagattga tcaaggagac ggtgatcgta
     6421 ggctttcaac tttatccgga tgcgattgtc gagtttactc actggttcct ccacttttag
     6481 cgcagaaggg ccccatcggg ataagctcac cgcaaggtaa gctctccgcg ggtgggagcg
     6541 cgatctttgc ctggttgtag gttctgcggg acaaggggta taaaccccct gccaccacga
     6601 tactctgacg agtatcctgg ttaaaggatg acatcgttcc tcctaaagaa ctataagtcc
     6661 ctgcaaaatc cgggacttgt gctgcacggt aaaaccgttt tattcagtaa ggtgcattca
     6721 ttttttatct cgggaatttg tcaaatggtt tttttgttgt tttagaccaa aagcccacgc
     6781 ctggtcagaa accagcgacc tggtgagcgc ggctctcgct tgtgagaagt tgacgccgtt
     6841 gtgactgcag gcctcccttt gaatcaatgc tcctgccgat aagcacgata accataatta
     6901 ttgcaatgaa cagcaacaac ttaacaaaat tggccgactt ccgctcccta attccttccc
     6961 gattagcatt acggacttat tacggactca ttacggacta agtccgtaat gagtccgtaa
     7021 taggatgccg ggcggaggcg gcaaggagtg cctaagcgct ttgtgctgtg gatgttgtgc
     7081 tgagaatttt gctgtggggt ttgctaacta tgtaaacacc atcgaacaag cgctgtcgca
     7141 ggctttagag cttgcacgaa agtggatatg gagagctggg ttgtcgatct gaaacttcgc
     7201 ccctggcggc gctttcaacg cctctttaca tgagacaata tactattttc acacaggttc
     7261 tgaagccagt aatgctgcac ccgccgattt tgaaatagac cccacaatct ctttgctctt
     7321 gggctctttt ttgaaatgat aagctctgtt ttctctgcta tctctgagat ttgttgttga
     7381 tgcccttgtc ccgcacccgc tgaagtcagt gcaacggcca gctaaagagc ttgtgcaaaa
     7441 atgccgtctt ggagtt
//
LOCUS       DAOSFN010000125         7417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_8636_length_7417_cov_9.168568, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000125 DAOSFN010000000
VERSION     DAOSFN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8636_length_7417_cov_9.168568"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLO35_07935"
     CDS             complement(<1..702)
                     /locus_tag="PLO35_07935"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOH79639.1"
                     /translation="MILTQTKQIHAQLLNCCWKALKRFCEFPLLSAALSSWDSGQDLH
                     LLAIGKDAGRMCEAAIKVLKDKVSDGFVLTKYGHYRCPDDRILHLEAGHPIPDDNSLQ
                     GSRLIVEWMSGLGRDSELIVLLSGGGSALFEMLNDGMDQAGLTSLNRALLRSSLGIAA
                     VNLERAKYSRLKAGGALTFFKGKQLKVFALSDVEHDDPAVIASGPFTPVHKDERGEYR
                     IIGNNMSFRTILAEEL"
     gene            858..2381
                     /locus_tag="PLO35_07940"
     CDS             858..2381
                     /locus_tag="PLO35_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOH79640.1"
                     /translation="MSDKVRETNSKAAMKELKEEYLRMLEESFQNTAEIKKGDVVDAP
                     IVSINETFIIVNLGGKFDAYAEIGEYADDKGELNLKVGDMLKGYIVDQNDQGYVVGKS
                     LTKQHVDKQSILDAFERKIPVSGKVYSLTKGGFNVDVLGARAFCPVSQMALRGVSDNA
                     ALIGKTMDFLVIECSENCRRIVVSHRQLEEAEANEKRAAAMEKLSVGAMVKGTVMRMT
                     SFGAFVDLGGVEGLMHVSEISWQHVVKPQDLLKTGQEVEVKVLDIKGDKIALSMKALM
                     DNPFTMALQELKEGDQINCRILRLHNFGAFAELKPGVEGLIPVSEMSRNRNVAHPREV
                     LKEGDFVQVQILRVDPDTQKISLSLKALQEDPWNKIDELVQIEQPFEGTVESSTNFGV
                     FVTITDGITGLLPRSRVRKADEFKSGEAITLMVTAIDKDNHRITLDYTDRTPEEIMES
                     QKREEGGHREPREQRGPRDYDSSRPPRKGGRSRQDEEWRKYANQKVSISSDNPFKDL"
     gene            2383..3879
                     /gene="lysS"
                     /locus_tag="PLO35_07945"
     CDS             2383..3879
                     /gene="lysS"
                     /locus_tag="PLO35_07945"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOH79641.1"
                     /translation="MQKPNQFIQLRLQKLARLRELGVDPYPVISKRSHKIGQVLRDRE
                     NWIESQQEITLTGRLVAMRRQGKLGFGNLEDESGKLQIYVQRDLVGEDNYEIFKLCDP
                     GDFVQTRGSMFHTQAGEYSIKVLEITMLGKNIRPLPAVKEKLEDGKLVRYDEFSDIEL
                     RYRKRYLDLLLNPSHRQVFETRSRIVSSTRRFLDERGFIEVETPVLQPLYGGANARPF
                     ITHHNTLDVDLYLRIATELYLKRLIVGGFERVYELGKDFRNEGMDRTHNPEFTMLELY
                     QAYTDLQGMMAITEDLFHHLAKDILQKSSYKFRGYDIQLDQPFTQAPMTELVSLHAGI
                     DLSDMDYHKAVAFCQEHKLEIPPGAGVGKLIALLFEHFVEPKLIQPTFVTDFPKEISP
                     LAKAKPGNPLLADRFELFIAGYEFGNAFSELNDPVDQRARLEAQAALRAQGDEEANVV
                     DEDFLEALEYGMPPMGGLGIGIDRMVMLLTENDSIKEVILFPQMKPEE"
     gene            complement(4005..4808)
                     /locus_tag="PLO35_07950"
     CDS             complement(4005..4808)
                     /locus_tag="PLO35_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79642.1"
                     /translation="MNSISKEMFLRLPAHEQTSRETLEKASTHTLLEVLLVTTVSQEA
                     RQRVADYLLSSRLSYDANESIVNQEVTLENYAQSDLGGMSLASAIDRLRPDVLLAILL
                     QPEYGAQIKQQVFQSLCARFSIRNTQPASIWTRTEEGTATASIPQEDLSALRRFHWGA
                     FFLAPLWCLMSGKAGTGVLMIFINFLARIPGLGIIFLICNYLIAYAIGKSAYEHLWEK
                     RKNKYGTMEQLIASQRGWVIVGCILGSLIAVYNVMAVISVISALSSGNL"
     assembly_gap    5333..5432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5455..7146)
                     /locus_tag="PLO35_07955"
     CDS             complement(5455..7146)
                     /locus_tag="PLO35_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOH79643.1"
                     /translation="MHILNLLGGLALFLFGMNKMSDNLQAVAGNEMRRILKMLTGTPF
                     RGVLVGLGVTALIQSSSATTVMMIGLVNAGIMTLNQAVGVIMGANIGTTITAQLIAFK
                     IGEYAFLFVIVGAVLLFMKRSRVMERWSAIILGFGLLFVGLNVMSDAVTPLKDSLVAQ
                     RVMADLSANPILGILTGMIFTMLIQSSSASIGIVIVLANTGLIGFEGAMYLVFGDNIG
                     TTITAWLAGLGANSTARRVALVHTMFNLFGTIIFGLLTYYGLYTVFVNKITPGNVFAG
                     ENIARHIANAHTFFNVLNTIVFLPFAGLLARIAEKLIPKGAGENLGMGEPKHLDYHLV
                     NDSELAIGQALKEMREMLRLVRMSLSISYEAFKEKNYRKQSRVAKIENAIDNLQREIT
                     RYLVTVNQRTNADNIIQKIPALLHTVNDIEKLGDFCEEINRILNFQIQSQKTPLCPDF
                     VAMIEDLEAKIMFMLDLSIEYLEELKPEYTYKIIEMEGRINEKHHNLREEILVTIQTG
                     NCDAVGGLNTIDFIDAMEVIGDKMKNLVKAGSHNFLYNEALKVIEADEGEDADLV"
BASE COUNT         1802 a         1955 c         1908 g         1652 t
ORIGIN      
        1 cagctcttcc gccagtatcg tcctgaaaga catattgttg cctatgatcc tatactcccc
       61 tctctcatct ttatgcacag gtgtaaaagg tccggaagcg atgacagccg gatcgtcatg
      121 ttcgacatcc gacagggcaa agaccttgag ctgcttgcct ttaaagaagg tcagcgctcc
      181 tccggctttg agccttgaat acttggccct ttccaggttg acggcagcta tcccgaggct
      241 gctccgaagc aaagccctgt ttagggaggt gagtccggct tggtccatgc catcattgag
      301 catctcaaac agggcggaac caccaccact cagcaggacg atcagttcag aatcccggcc
      361 cagcccagac atccattcca cgatcaagcg gctgccttgc agagagttat catccggaat
      421 gggatgcccg gcttccagat gaaggatgcg gtcatctgga cagcggtaat gcccgtattt
      481 ggtgaggaca aagccatccg agaccttgtc tttcagcacc ttaatggcag cctcacacat
      541 gcggcctgcg tcttttccta tggccagcag atggaggtcc tgtccggaat cccaggagga
      601 cagggcagcg ctgagaagcg gaaactcgca gaagcgcttt aacgctttcc agcagcagtt
      661 tagcaactgc gcgtgaatct gttttgtttg cgtgaggatc atgaaaggat gttattcacc
      721 acggccctga gacgtcaagc ggaatcttaa agcggtctcc cttatggcga taatcactcc
      781 caacgcaata aagtacttta caaaaacagg ctcctcaaaa acgtgggtct ttctttaata
      841 gtcgttagga gttacttatg tcagacaagg tgcgtgaaac caacagcaaa gccgcgatga
      901 aggaattgaa agaagaatat cttcgcatgc tcgaagagtc ctttcaaaac accgcggaga
      961 tcaaaaaagg cgacgtcgtg gacgccccca tcgtgagcat caatgaaacc tttatcattg
     1021 tgaacctggg cggaaagttc gacgcgtatg ccgagatcgg cgaatatgct gatgataagg
     1081 gagagctcaa cctcaaggta ggagatatgc tgaaaggcta tatcgtagat cagaacgatc
     1141 aaggctacgt tgtgggcaag agcctcacca aacaacatgt agacaagcag tccattctgg
     1201 acgcgttcga aaggaagatc cccgtatcgg gtaaggtcta ttccctcacc aaaggcggtt
     1261 ttaacgtgga cgtcctgggt gccagagctt tttgccccgt cagccagatg gccctgcgcg
     1321 gagtatccga caacgccgct ctcatcggaa agaccatgga tttcctcgtg atcgaatgct
     1381 cggaaaactg ccgtcgcatt gtggtttccc accgtcaact ggaagaggct gaggccaatg
     1441 aaaaacgggc cgccgccatg gagaagctca gcgtgggcgc catggtcaaa ggcaccgtga
     1501 tgcgcatgac cagcttcggc gctttcgtcg atctgggtgg cgtggaaggc ctgatgcatg
     1561 tttccgagat ttcctggcag catgtggtca aacctcagga tctcctcaag accggtcagg
     1621 aagtggaagt gaaagtgctg gacatcaagg gagacaagat cgccctctcg atgaaagcct
     1681 tgatggacaa ccccttcact atggccctgc aggagctcaa ggaaggcgac cagatcaatt
     1741 gccgcatcct caggctgcac aactttggcg cctttgccga gttgaaacca ggcgtcgaag
     1801 gcctgatccc cgtcagtgaa atgagccgca accgcaatgt ggcccatccc cgcgaagtgc
     1861 tcaaggaagg ggactttgtc caggtgcaga ttctgcgcgt ggatcccgat acccaaaaga
     1921 tttccctctc cctcaaagcc ctgcaggaag atccctggaa caaaattgac gagctcgtcc
     1981 agatcgaaca gcccttcgaa ggcacggtgg aaagctccac caacttcggc gtgtttgtca
     2041 ccatcacaga cggaatcact ggtctgctgc cgcgttccag agtccgcaaa gccgatgaat
     2101 tcaagagcgg agaagccatc accctgatgg tcaccgccat agataaagat aatcaccgca
     2161 tcaccctgga ttacaccgac cgcactcccg aagagatcat ggaaagccag aaacgcgaag
     2221 aaggcgggca tcgtgagcct cgcgaacagc gtggcccccg tgactatgac agcagccgtc
     2281 ctccccgcaa gggtggacgc agccgccagg atgaagaatg gcgcaaatat gccaatcaaa
     2341 aggtgagcat ctcctcggac aatcctttca aagacctgta aaatgcagaa acccaatcag
     2401 tttatccagc ttcgcctgca aaagctggcc cgccttcgcg agctgggagt agatccctat
     2461 ccggtgatct caaagcgcag ccacaagatt ggccaggtgc tgcgggaccg ggaaaactgg
     2521 atcgaaagcc agcaggagat cactctcacc ggtcgcctgg tggccatgcg ccgtcaggga
     2581 aaactgggct ttggcaatct cgaagacgaa agcggtaagc tgcagatcta cgttcagcgc
     2641 gatctggtgg gcgaagacaa ttatgagatc ttcaagctct gcgatcccgg agactttgtc
     2701 cagacacgcg gcagcatgtt tcacacccag gcgggagaat acagcatcaa ggtccttgag
     2761 atcaccatgc tgggcaaaaa catccgtccc ctgcccgccg tgaaagagaa gctggaagac
     2821 ggcaagctgg tccgctatga cgagttttcc gatatcgaac tgcgctaccg caagcgctat
     2881 ctggatttac tgctcaaccc cagtcaccgt caggtgtttg agacgcggtc caggatcgtg
     2941 agctccacgc gcaggtttct ggatgaacgt ggctttatcg aagtggaaac ccctgtgctg
     3001 caaccgcttt acggaggcgc caatgcccgg cccttcatca cccatcacaa cactctggat
     3061 gtggacctct atctgcggat cgccaccgag ttgtatctca aacgcctcat cgtgggaggg
     3121 tttgagcggg tgtatgaact gggcaaggat ttccgcaatg aaggcatgga ccgcacccac
     3181 aatcccgaat ttaccatgct ggaactctat caggcctaca cagacttgca gggcatgatg
     3241 gctatcacgg aagatctctt ccaccacctc gccaaagata tcctgcaaaa atccagttat
     3301 aagttccgcg gttacgacat ccaactggac cagccgttca cccaagcccc gatgaccgaa
     3361 ctggtgagct tgcatgccgg catcgacctc agcgatatgg actatcacaa agccgtggct
     3421 ttctgccaag agcacaagct ggaaattcct ccgggcgcgg gagtaggcaa gctgattgcc
     3481 ctgctcttcg agcattttgt ggagcccaag ctgatccagc ctaccttcgt gaccgatttc
     3541 cccaaggaga tatctcccct ggccaaggct aaacccggta atcctttact ggcagaccgc
     3601 ttcgagctct tcatcgccgg ttatgaattt ggcaacgctt tcagcgagct caacgacccc
     3661 gtggaccagc gcgcccgtct ggaagcccag gcagcgctgc gggcccaggg cgacgaagaa
     3721 gccaacgtgg tggacgaaga cttcctggaa gccctggaat acggcatgcc acccatgggc
     3781 ggactgggca tcggcatcga ccgcatggtg atgctgctca ccgaaaacga ttctatcaaa
     3841 gaagtgatcc tctttcccca gatgaaacct gaggagtaga gcaaaacttc acctataatg
     3901 aagcgcggcc ggcctgggtc gcgctttttc gcgttcagga tatcctcaga agctctatcg
     3961 atgtgccccg cggcaattca ttgtcatgca tttcactagt ggaatcagag attgccagat
     4021 gagagcgcgg agatcacaga tatcacggcc atgacgttgt aaactgcaat cagcgaaccc
     4081 aggatgcatc ccacaatcac ccatcctctc tgtgaagcta tcagctgctc cattgtgcca
     4141 tacttatttt tgcgtttttc ccagagatgt tcgtaagcgc ttttacctat agcatacgca
     4201 atcagatagt tgcagatcag gaaaatgatg cccaggcccg ggattcgggc cagaaaattg
     4261 atgaagatca tcaaaacccc ggtgccggcc tttccgctca tcagacacca gagcggggcc
     4321 agaaaaaagg ctccccaatg aaaacgccgc aaagcgctga ggtcttcctg tgggattgac
     4381 gcggtagccg ttccttcttc tgttctggtc caaatgctgg ccggttgggt gtttctgata
     4441 ctgaatctgg cgcacaggct ttgaaatacc tgttgcttga tctgagcccc atattcaggt
     4501 tggagcagta tcgccagcag cacatcagga cggaggcggt cgatcgcgct ggctaaactc
     4561 atgccgccca aatctgattg cgcatagttt tcaagcgtga cctcctgatt gacaatactc
     4621 tcattggcgt cgtaggacaa tctgcttgaa agcaggtaat ctgctaccct ttggcgtgcc
     4681 tcctgactga ccgtcgtaac caaaaggacc tccagcagcg tgtgggtgga ggctttttcc
     4741 aaggtttcac gcgaggtctg ctcatgagcc ggcagcctca aaaacatctc tttagagata
     4801 ctgttcatac tacactccca tatcttgatt tatccttgcg ttgacatctt gagtcgaaca
     4861 tgaatctgtc aagctaattc tcggaaattt gcacatttct tcgtcgcacg gagatataag
     4921 ccataaacag acctatgtgt ctgtggggca tccaattatg cccatgctac cgacgctctt
     4981 atcgccgcta gttgccaggt ttgcgacacc cttggccccc ttctgctcca cctttgccaa
     5041 agtaaaggag aggcaaagga gaggcatagg tggtgtaaag gaggctggag ggagtgatgg
     5101 gtgaagggtt gaagagtgaa gggtgaatag agaacgctcg ctgggggagc cggaaacgtc
     5161 ctcgtttccg gaggcctgta gcgtcgcttt ccaaatcggc ggcgggtttt aaatactgag
     5221 ctacagaggt tttccttttt cactacagag gaacgaagga aacagagaga gatacagagg
     5281 atttataact agaaattgac cccctcttca ctattcaccc ttcacccttc acnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcaccct tcactcttca ccattcacac
     5461 caggtccgcg tcctctccct catccgcttc gatcactttc agggcttcgt tatagaggaa
     5521 gttgtgggat ccagccttca cgaggttttt catcttgtcg ccgatcactt ccatggcatc
     5581 gataaagtcg atggtattca gaccaccgac cgcgtcgcaa ttgccggttt ggatagtgac
     5641 caggatctcc tcgcggaggt tgtggtgctt ttcattgatg cggccttcca tctcaataat
     5701 cttgtaggtg tattccggtt tcagctcttc caggtattcg atggagaggt ccagcataaa
     5761 catgatcttg gcttccagat cttcgatcat ggccacaaaa tcgggacaca ggggagtttt
     5821 ttgagattgg atctggaagt tgaggatacg gttgatttct tcgcaaaagt cacccagctt
     5881 ttcgatatcg ttgacggtat gcagcagagc gggaatcttt tggatgatgt tatcggcatt
     5941 ggtgcgctga ttgacggtca ccagatagcg ggtgatctct ctctggagat tgtcaatggc
     6001 gttttcgatc ttggccacgc ggctctgctt gcgatagttt ttttctttaa aggcctcgta
     6061 ggagatactc aggctcattc tcacgaggcg cagcatttcc cgcatctctt tgagggcctg
     6121 tccgatagct agctcggaat cgttcacaag gtggtaatcg aggtgttttg gttcgcccat
     6181 tcccaaattc tcacccgccc ctttggggat gagcttttcc gcgatcctgg ccaggagccc
     6241 ggcaaaaggt aaaaagacga tggtgttgag cacgttgaag aaggtgtggg cgttggcaat
     6301 atgacgggcg atattttccc ccgcgaagac attgccgggc gtgattttat tcacaaacac
     6361 ggtatagagg ccatagtagg tgagtaaacc gaagataatg gtgccaaaga ggttgaacat
     6421 ggtgtgcacc aaagctaccc tgcgggctgt gctgttggca cccagacctg ccagccaggc
     6481 tgtgatagtg gtgccgatat tgtctccaaa caccagatac atcgcccctt cgaaaccgat
     6541 caggcctgta ttggccagca ctatcacgat cccaatggaa gcggaagagc tctggatcag
     6601 catcgtgaag atcatacccg tgagaatgcc gagaatggga tttgctgaga gatcagccat
     6661 gaccctctga gcaaccagcg agtctttgag aggcgtaacg gcatcagaca tcacattcag
     6721 gcccacaaac aacaggccaa atcccagaat gatagcactc cagcgttcca tgacgcggct
     6781 gcgcttcata aacaacagca cagctcccac gattacaaag agaaacgcgt attcgccgat
     6841 cttgaaggca atcaattggg ctgtgatggt ggtgccgata ttggcaccca tgattacgcc
     6901 tacagcctga ttgagcgtca ttatccccgc gtttaccagg ccgatcatca tcaccgtggt
     6961 ggcagaactg ctctggatca aagctgtgac gcccaatccc accagcacac ccctgaaggg
     7021 ggtaccagtc agcatcttga gaatgcggcg catctcgttg cctgccacag cctgaaggtt
     7081 gtcgctcatc ttgttcattc caaacagaaa gagcgctaaa ccgcccagaa gattcaatat
     7141 gtgcataaag ctcattgttt cgcaacctat ccagtgttaa gatgcctcaa tttctgatgg
     7201 ggggcatatc ggtcaaggga attgttagga tttgataaag ggtcccaaaa gatcatcgcg
     7261 ccagacccca ttgctggtct tgtgactttt ttgaatggcg gatggaggga tggcagttta
     7321 gagactggtg cacgtaaacg gatcactggc ttcagccggt cgtagcactg acttcagagc
     7381 ttttgcaaaa actggcattt tgagttccgt aggaacg
//
LOCUS       DAOSFN010000126         7296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_8852_length_7296_cov_9.381577, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000126 DAOSFN010000000
VERSION     DAOSFN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8852_length_7296_cov_9.381577"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PLO35_07960"
     CDS             <1..778
                     /locus_tag="PLO35_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOH79644.1"
                     /translation="RETMVESMIFFGVFLGVVLLNITFHLQSRLFHAGVIYIQIILDT
                     VFATIVVHITGGLNSYFVWVYLIAVITASLTIPKVGGIFAGLCGSLSLLTLTALYQNG
                     ILSPTQISEVDATNSTIYVLSYTGLFSGVAFIASYMNEFIIKHRLAESDLQQTRQNLD
                     EVQQQLDHCLDAKQKFTNLHPILQSVGQLKHDINTPLCVITLSLSRVKRAGQELNHEG
                     LQKSNNEITEAVNRITSILGRVDELNARIKHFEEEVESDT"
     gene            768..1613
                     /locus_tag="PLO35_07965"
     CDS             768..1613
                     /locus_tag="PLO35_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH79645.1"
                     /translation="MIRKKILVADDEPNIREIISELLREAGHRVVVAVDGLDAWEKMQ
                     MEPYDLYVLDVFMPRLDGLDLIEKIRELQPMAVIIVITGFSSIDVAIKAIRKGAYHYI
                     TKPINADELIKAVEAGLKYVDELQEDTLSEGTQSAEPATDLALMRGFTPEQQQDFKLM
                     GTIDHYAPGQHIPVDDETGSLIWVESGRINVMYNGSVVDSIQDGEIWGEETFISASAA
                     FTELIAQSEVQIRRFKRKRIIEFFTYQDEHLTNRYMINLFQSIYLKWRKSVHKLGLFS
                     GFYKG"
     gene            1618..2829
                     /locus_tag="PLO35_07970"
     CDS             1618..2829
                     /locus_tag="PLO35_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOH79646.1"
                     /translation="MPLSYSDQLRAQIPEVLYGPDLCENLNQWLGDKPEYVTEARELM
                     HEWLRDMRDRKASDLDFGAPGANCKIWTRIYGMKRPVETYESYPTLDTTAIILSILTP
                     SQKVILVKDFSLDFSYCLDVDGSQVRFRGTAYFDRGFLCVNFRRINENIYTMESLGIP
                     RPIAEKMNLKHEKTGLILITGITGSGKSTTLDAIIDMNNRDNPAHIVIIGHPIEHVHD
                     SKLSMVRHREVGEDVRSFEYGAIEALRQDPDIIVVGEMRDPRTIATVLEITDSGHKAF
                     TTLHTSSAIDSIHRIIAEFPPEEQERIRNRLADVLSITMSQKLIPTVDHKLVMTKEIL
                     AVDASVQAAIRNKNIGEIYQMMIEGRRYGMCTLEQDLQDLFKRGIITNITAMNYANNK
                     KRMAQLLTAGF"
     gene            2852..4990
                     /locus_tag="PLO35_07975"
     CDS             2852..4990
                     /locus_tag="PLO35_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79647.1"
                     /translation="MVKHPKQAFGISEDGTVLRLVRLIRDRDQVYLHDVDRIDLERSL
                     YNLQEELPVAAETKAWEDEGGSDQEIKLDEFENVYSPDIKTSPWETMFNSYYLRQGVL
                     ALNISEEHLIKAQEQIEGARKVKQYVKSQLSPQEFKAKEWQTCQVKINQRPCLWLHKG
                     PNQLFNILAEYARKHKHQFYYQLADSNDIALTDYFRVNCLEKDKRTLLVYLGVEYRRA
                     FMFDKGEWITTLPLQITQSAPTPEIVYSKLALALDSAQLSDPEQLVICGDLVNAELLE
                     YLSAQYPPNTVSILNFAHIVVNSQRSDLYENIYLAQFALPLALAYKALFPEDLRYTHS
                     NFLPHRTLESQKTFKVAWHGLIVLGIIFLLTLFGTVSMLKVSSRLKSAKTNKRVLDHT
                     LAQRRIEAAEIQEIRTKLEQQQKVFEVMRGILDGKNPWTEVLDILNRKVASLPITWLT
                     NLKKSGERLVITGITTNRQHILSIANALPNSGIIKVTEAEIKDRRVWDFEIQSDLPKK
                     DWIAEIENDLRKLMDMKQQYGEDPSQTGSPEAPKGKAAAATGPQKASTAQVKNLAPYR
                     GLSPLLDKYMPQVPDNIIKKGGDDLKEYYAFIASINRGNMWEYRDLGVKFLNRHSSGT
                     LADLVRWYMAYRLYIDKEFSLVHVYNEPLISSSTELYPWALLLAARVDYARNSAGYKN
                     RYDNLKNDYSRHIISGQIKEDLRVIEGAAK"
     gene            4987..5847
                     /locus_tag="PLO35_07980"
     CDS             4987..5847
                     /locus_tag="PLO35_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79648.1"
                     /translation="MSNALRNSVILTILLILMLSGFLLFHRRYNKQAGILEAQNKKTE
                     ATIADLDEQISKRDSLMAAYEIMLEMQSQQSKILVGSDSANQSYQYLLRVLKWMGKYI
                     PFNFALTDASKKQAAYNEYVISGRTHYSDLIKLTKHLEYERAVITIEDFSIGSDTVAA
                     SDTVSFSMVFRTHFQEGGPELESITNKDFNSPWLGYQLFNIRIYDSIPEREIDPRLVN
                     ISDATLIGLGDGKAFLRDKQGIIHILSPGSKVAYGYLREVDMPNGRAVFKLNLHGLEE
                     THFLQVRNLP"
     gene            5855..6967
                     /locus_tag="PLO35_07985"
     CDS             5855..6967
                     /locus_tag="PLO35_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HOH79649.1"
                     /translation="MKYIISLMILICALALMAAPPKPDDTIVTLGENIHINDAIRVLE
                     TFSMKFESKKIMNLSSHNSAIGFPINSLNWRRALELIALKNNLTVEELPGYIAIKDFV
                     SEGASVEARTVNDLLDTKLVKIEAKALLADKAYLKSLGIDWSTIFNGRVQIEAGFSGG
                     SQVPNNLMNLAASRTVSWDGKTIDINTLLNVIESNQKGTVIAQPTLLVSSGKRGFIQV
                     GQDISVKTVDEAGNTTDTFFATGVIMDVTPTIVKVDTIEAVHLVTSIERSSGTPGNVS
                     TIINRSKSDTDLVLYSGEEIVIGGLYDTDEIRTRSGIPILKDLPWWVFGIRYLTGYYK
                     YEKKERELVIIMKVEIVPNVVERQKMDKLEKQAAER"
BASE COUNT         1910 a         2017 c         1850 g         1519 t
ORIGIN      
        1 gagggaaacg atggtggagt cgatgatttt ctttggcgtg tttttgggtg tggtgttgct
       61 caatatcact tttcacctgc agtcgcgttt gtttcatgcc ggcgtcatat acatacagat
      121 tattctggac accgtttttg ccaccattgt ggtgcatatc accggtggac tcaacagcta
      181 ctttgtgtgg gtctatctga tcgccgtgat taccgcgtcg ctcactattc ccaaggtggg
      241 aggcatcttt gccggtctgt gcggcagcct ctcgttgctt acgcttacgg ccctttatca
      301 gaacgggatt ttatccccca cccagatatc tgaagtggat gccaccaatt ccaccatcta
      361 cgtgctctcc tacacgggac tgtttagtgg agtggccttc atcgcctcat atatgaacga
      421 gtttatcatc aaacaccgcc tggccgaatc ggacttgcag cagacgcggc aaaacctgga
      481 cgaggtccag cagcagctgg accattgcct ggatgcgaag cagaagttca ccaacctcca
      541 tcccatcctg cagagcgtcg gccagctcaa gcatgacatc aacaccccct tatgcgtgat
      601 caccctgtcg ctctcgagag tcaaacgggc cgggcaggaa ctcaatcacg agggcttgca
      661 gaagagcaac aacgagatca ccgaggccgt gaaccggatc acttccatcc tgggaagggt
      721 ggatgaactg aacgcacgga tcaagcactt cgaagaagag gtagaaagtg atacgtaaga
      781 aaattttagt agccgatgac gaacccaaca tccgggagat tatcagcgaa ctgctcaggg
      841 aagccggaca ccgagtagta gtagccgtcg atggcctgga tgcctgggaa aagatgcaga
      901 tggagcccta tgatctctat gtgctggatg tctttatgcc cagactggac ggattggacc
      961 tgatcgagaa gatccgggaa ctgcagccca tggccgtgat catcgtgatc acaggtttct
     1021 ccagcatcga cgtggccatc aaggccatcc gcaaaggggc ttaccactac atcaccaagc
     1081 ccatcaacgc ggatgaactg atcaaagccg tcgaggccgg cctcaagtat gtggatgagc
     1141 tgcaggagga tacgctatcc gaaggtactc agtctgccga gccggccacg gaccttgctc
     1201 tgatgagggg cttcactccg gaacagcagc aggatttcaa gctcatgggt accatcgatc
     1261 attacgcgcc ggggcagcac atccccgtcg atgacgaaac ggggtcgctg atttgggtgg
     1321 aaagcggtcg gatcaacgtg atgtacaacg gctcggtggt ggattccatt caggatggag
     1381 agatctgggg cgaagagact ttcatcagtg ccagcgctgc ctttactgag ctgattgccc
     1441 agtccgaggt ccagattcgc cgcttcaaac gcaagcggat catcgagttc tttacctatc
     1501 aggatgagca cctcaccaac cgttatatga tcaacctctt ccagagcatc tacctcaagt
     1561 ggcgcaagtc cgtccacaag ctgggccttt tcagcggctt ttataaaggc tgacgcgatg
     1621 cccttaagct attctgacca gctccgggcc cagatcccgg aggtcctcta cggaccggac
     1681 ttgtgtgaaa acctgaacca atggctggga gacaaacctg aatatgtgac cgaagcccgt
     1741 gaactgatgc acgaatggct gagggatatg cgggatcgta aagcttcaga cctggatttt
     1801 ggcgctccag gggccaactg caaaatatgg accagaatct acggtatgaa acgtccggtg
     1861 gaaacctatg aaagttatcc tactctggat actactgcca tcatcctctc gattctcact
     1921 ccctcccaga aagtcatcct ggtaaaggac ttcagtctgg atttctccta ttgcctggat
     1981 gtggatggtt cccaagtcag attccgtggc accgcctatt ttgacagggg ttttctatgc
     2041 gtcaatttcc gccgcatcaa cgaaaacatc tacaccatgg aaagcctcgg cattcccagg
     2101 cccatagccg agaagatgaa tctaaagcat gaaaagaccg gcctaatcct gattaccggc
     2161 atcaccggca gcggtaaaag caccactctg gatgccatca tcgatatgaa caatcgggac
     2221 aacccggccc acattgtgat catcggtcac ccgatcgagc atgtacacga ttccaagctc
     2281 agtatggtgc gccatcgcga agtgggagag gacgttcgct cctttgaata cggtgccatc
     2341 gaagccctgc gccaggatcc cgatatcatc gtggtgggtg aaatgcgcga tcccaggact
     2401 atcgccaccg tcctggagat caccgacagc ggtcacaaag ccttcaccac cctccacacc
     2461 agttccgcca tcgacagcat ccacaggatc atagctgaat ttcctcccga agagcaggaa
     2521 cgtattcgca accgtctggc cgacgtgctg agcatcacaa tgtctcaaaa actgatcccg
     2581 acggtggacc acaaactggt aatgaccaag gaaatcctgg ctgtggacgc atccgtccag
     2641 gccgccatcc gcaataaaaa catcggcgaa atctatcaga tgatgatcga aggccgccgt
     2701 tatggaatgt gcactttgga acaagatctg caggatctct ttaaacgcgg gattatcacc
     2761 aatataaccg ccatgaatta tgccaacaac aaaaaacgca tggcccagct gctcacagcg
     2821 ggattctgat cagtacaggt aaagagaaga tatggtaaaa cacccaaaac aagcctttgg
     2881 aatatctgaa gacggcaccg tcctacgtct ggtgcgtctg atcagggacc gggaccaggt
     2941 ttacctgcat gatgtggacc ggatcgatct ggagcgctcg ctctataatc tccaggaaga
     3001 acttcccgtg gcggcagaga ccaaagcctg ggaagatgag ggtggatctg atcaggaaat
     3061 caagctggat gaatttgaaa acgtctatag cccggatatc aaaaccagcc cctgggagac
     3121 gatgttcaat tcctattacc tcagacaggg tgtgctcgcc ctcaacatca gcgaagagca
     3181 tctcatcaag gcccaggagc agattgaggg ggccaggaag gtcaagcagt acgtcaaatc
     3241 ccagctgagc cctcaggagt ttaaggccaa agagtggcag acctgccagg tcaagatcaa
     3301 ccagaggccc tgtctctggc tgcacaaagg gcccaatcag ctttttaata tcctcgctga
     3361 atacgcccga aagcacaaac atcagttcta ttatcaactg gcggactcca acgacatcgc
     3421 cttgaccgat tatttcaggg tgaactgcct tgaaaaagac aaacgcactc tgttggtcta
     3481 tctgggagtg gagtatcggc gtgccttcat gtttgacaag ggagaatgga tcaccacgct
     3541 gcctctgcag atcactcaaa gcgctcccac cccagagatc gtgtattcca agctggccct
     3601 ggccctggac agcgcgcagc tcagcgatcc agagcaactg gtgatttgcg gagacctcgt
     3661 aaacgccgaa ctgctggagt atctcagcgc tcaatatcct cccaacacgg tcagtatcct
     3721 caattttgcg cacattgtgg tcaacagcca gaggtccgac ctctatgaaa acatctatct
     3781 ggcccagttc gcgctccctt tggccctggc ctataaagcc ctatttcctg aggacttgcg
     3841 ttatacccac agcaatttcc tgcctcacag gaccctggag agccagaaga ccttcaaggt
     3901 ggcctggcat gggttgatcg ttttgggcat cattttcctg cttactttgt ttggcaccgt
     3961 atccatgcta aaagtctcat cgcggctaaa aagcgccaaa accaacaagc gcgtcctgga
     4021 tcacaccctg gcccaaagaa ggatcgaggc agccgagatt caggagattc gcaccaaact
     4081 tgagcaacag cagaaagtct ttgaagtgat gcgcggcatt ctggacggca agaacccctg
     4141 gaccgaggtg ctggacatcc tcaaccgcaa ggtggcctcc ctgcccatca cctggctcac
     4201 caacctgaag aagagcggtg aacgcctggt cattacaggc atcaccacca accggcagca
     4261 catcctcagt attgccaacg cgcttcccaa cagcgggatc atcaaggtca ccgaagcgga
     4321 gatcaaagat cgccgcgtct gggattttga gatccaatcc gatctgccta aaaaagactg
     4381 gatcgccgag atcgaaaacg acctgcgcaa gctgatggac atgaagcagc aatacggcga
     4441 agatcccagc cagacaggct ctccagaagc cccaaaaggc aaagcggccg ctgcgaccgg
     4501 acctcagaaa gcaagcaccg cccaggtcaa aaacctggcc ccttaccggg gattgagtcc
     4561 ccttctggac aaatacatgc cccaggtgcc ggataatatc attaaaaagg gcggtgacga
     4621 cctcaaagag tattacgcct tcatcgcttc catcaaccgg ggcaacatgt gggaatacag
     4681 agacctggga gtcaaattcc tgaacaggca cagcagcggt accctcgcgg acctggtgcg
     4741 ctggtatatg gcctaccggc tctatatcga caaagaattc agcctggtgc acgtgtacaa
     4801 cgaacccttg atctccagct ccacggaatt gtatccctgg gccttgttgc tggcggccag
     4861 agtggattat gccagaaact ccgcgggata caagaaccgg tatgacaatc tcaaaaacga
     4921 ctactcgcgg cacattatca gcggtcagat caaagaagac ctcagagtga tcgaaggagc
     4981 ggccaaatga gcaacgcgct gcgcaactcc gtcatcctca ccatcctgct gattctgatg
     5041 ctgtccgggt ttctgctgtt ccacagacgc tacaataaac aggcgggtat cctggaagcc
     5101 cagaataaaa agacggaagc caccatcgcc gatctggacg aacagatcag caaacgcgat
     5161 tccctgatgg cggcctatga gatcatgctg gagatgcaga gccagcagag caagatcctg
     5221 gtgggatcgg attccgccaa tcaaagctat cagtatctgc tcagagtcct gaagtggatg
     5281 ggcaaatata tccccttcaa tttcgccctc accgatgcct ccaaaaaaca ggccgcctac
     5341 aacgaatacg tgatcagcgg ccgcactcac tactctgatc tgatcaagct caccaagcat
     5401 ctggagtatg aacgggcggt gatcaccatc gaagacttca gcatcggttc ggacaccgtc
     5461 gcggccagtg acaccgtgag cttttccatg gtgttcagga ctcatttcca ggagggtggc
     5521 ccggaactgg aatccatcac caataaagat ttcaacagcc cctggctggg atatcagctc
     5581 ttcaacatcc gaatctatga cagcatcccg gaacgcgaga tcgatcccag actggtgaat
     5641 atctcggatg ccaccctgat tggtctgggc gatggaaaag ccttcctgag agacaaacag
     5701 gggatcatcc acatcctgtc tcccggcagc aaagtggctt acggctatct gagagaggtg
     5761 gacatgccca acggcagggc tgtcttcaag ctcaatttac acgggctgga agagacccac
     5821 ttcctccaag taagaaatct tccatgaggt taacatgaaa tatatcatca gcttaatgat
     5881 cctgatctgc gccctggcgc tcatggcggc ccctcccaaa ccagacgaca ccatcgtcac
     5941 cctgggagag aatatccaca tcaacgatgc catcagagtg ctggaaacct tctccatgaa
     6001 gtttgaaagc aaaaaaatca tgaacctgtc cagccacaac agcgctatcg gcttccccat
     6061 caacagtttg aactggcgcc gcgccctgga attgatcgcc ctgaaaaaca accttacggt
     6121 ggaagaactt cccggctata tcgccattaa ggattttgtc tccgaaggcg ccagcgttga
     6181 ggcccgaacc gtcaacgacc tgctggatac caagctcgtc aagatcgagg ccaaagccct
     6241 gctggccgac aaagcctatc tgaagtctct gggcatcgac tggtccacca ttttcaacgg
     6301 acgggtccag atcgaagcgg gtttttcggg aggctctcag gttcccaaca atctcatgaa
     6361 tctggccgct tccaggaccg tctcctggga tggtaaaacc atagatatca acacactgct
     6421 caacgtgatc gaatccaacc aaaagggtac cgtgatcgct cagcccaccc tgctggtcag
     6481 ctccggtaag cgtggattca tccaggtggg ccaggatata tccgtcaaaa ccgtcgatga
     6541 ggccggcaac accaccgata ccttcttcgc cactggtgtg attatggatg tgacccccac
     6601 catcgtgaaa gtggacacga tcgaggccgt ccatctggtc accagcatcg aacgcagttc
     6661 tggaactccc ggaaacgtga gcaccatcat caacaggagc aaatctgaca cggacctggt
     6721 gctttacagc ggagaagaga tcgtgatcgg gggcctctat gacaccgatg agattcgcac
     6781 ccgcagcggt atccctatcc tcaaagacct gccctggtgg gtgtttggga tccgttatct
     6841 gaccggctac tataaatacg aaaagaaaga acgcgagctg gtgatcatca tgaaggtgga
     6901 gatcgtgccc aacgtggtgg agagacagaa gatggacaag ctggaaaagc aggctgctga
     6961 acggtaagcg agtgcctggc ctcagagcat tagagtatac tggcgaagcc aaagcctgac
     7021 atgcccccgt tggagttcaa gcggccacga taaaggtatg cgtggcaacg agtttagggc
     7081 agaattcacc tcacgttttg agccccgtca ggggcggcat ttcagctaga ctatgcgtat
     7141 ccaagcttgg attgagctcc gtcaggggcg cagagtgttg acaaagccct gttccaaaac
     7201 cgagaaaaca ttactggaat aaccaacaaa gtaatagaaa tgacagataa ccatgtgatt
     7261 aatgacttta ggacgccttg atgtcttttc tttttg
//
LOCUS       DAOSFN010000127         7257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_8924_length_7257_cov_10.276868, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000127 DAOSFN010000000
VERSION     DAOSFN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8924_length_7257_cov_10.276868"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..410)
                     /locus_tag="PLO35_07990"
     CDS             complement(72..410)
                     /locus_tag="PLO35_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOH79650.1"
                     /translation="MATQAATAELILSQIHSAGESKVLKMFGEYGLYCDGKIVALICD
                     DQLFIKPTEAGQTFLGEVETAPPYPGAKDYFLIPEDMWEEPGFLAELVRVTADALPAP
                     KPKKPRAKAR"
     gene            complement(559..1071)
                     /locus_tag="PLO35_07995"
     CDS             complement(559..1071)
                     /locus_tag="PLO35_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79651.1"
                     /translation="MRRALICLLLLLSLLMMFGCKGKNGEEGKTFLKVWLINCTRYWD
                     NNPGIPFGFNTTTFYLCNPGTYDYEYDTSDGWEWWGTYTLVAEEGEEGGLFQNGDDGE
                     DRRYTFTCQSSGPTLTYTVGKGKDAKLLTPVQVGEDEFEINYSDGKYRFEVKAFRRAS
                     TGGTRTKLSR"
     gene            complement(1290..1742)
                     /gene="purE"
                     /locus_tag="PLO35_08000"
     CDS             complement(1290..1742)
                     /gene="purE"
                     /locus_tag="PLO35_08000"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOH79652.1"
                     /translation="MPQVRVILGSKSDLESCQPIKDTLAEFEVDYDFHVSSAHRKPEQ
                     TALLAKNAASEGVKVIIACAGMAAHLPGVVASHTHLPVIGVPLAGGALNGVDALYSVA
                     QMPPGVPVACVAINGAKNAALLAIQILGTGDPVLRDKLIEYKRLLALG"
     gene            complement(1742..2416)
                     /locus_tag="PLO35_08005"
     CDS             complement(1742..2416)
                     /locus_tag="PLO35_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOH79653.1"
                     /translation="MAKKFLSRADAAKKLKVSEKVIQEMINSKVFESKLVGKNVKIDE
                     DSLNEWLENLNESEEKMLALKRVICHFEEYMRPENIFLDFAAENKYDAIRVLSEKAKD
                     LKLVRDARWLYEVVVAREELISTAIGNGVALLHPRHLHPSKIKVPSILFGRSLEGVDF
                     DAPDNKPVNIFFMLLLHNDKQHLFSLSYISKLIMNPEILASFSSAADKEAIHEALTVL
                     PETQNR"
     gene            2574..3011
                     /locus_tag="PLO35_08010"
     CDS             2574..3011
                     /locus_tag="PLO35_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOH79654.1"
                     /translation="MYSNAYPHLKKIRVAPFENRSAEFDIADQVFTGLNRQFRNDGRL
                     KPVTQAPDCQLEGVITSFEERVYSYDTGNNIQDYQLRLTLSVTFSDLVRNEIIWENKS
                     LTLSETYAVSEASTAKFKSKEEATDELIQSLFRTIVQNSLERW"
     gene            2953..3780
                     /locus_tag="PLO35_08015"
     CDS             2953..3780
                     /locus_tag="PLO35_08015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOH79655.1"
                     /translation="MSSSRVSSGRSSKTASNAGEGIRLNRFLAENGLGSRRKTEELIS
                     SGRVSINGQTVTDLGRRVNPLQDEVKVDGKALGITKELLYLVLNKPRGYVVSHADEQG
                     RDTIYKLLPVEAANLNYAGRLDKNSEGLLLLTNDGDMINLLTHPNFKVEKVYKVDVTP
                     RLSRAAITQLREGVAIEGGMTLSAGVYVKSEHEGGMTLKVVIREGRKRQIRQMVEAVG
                     GKVKGLKRLQFGPLMLKNLATGAWRPLNPIELKALRYICDKGKKLHEDRPHRPAQKR"
     gene            3743..4816
                     /locus_tag="PLO35_08020"
     CDS             3743..4816
                     /locus_tag="PLO35_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOH79656.1"
                     /translation="MKIALIGLPKSGKTTIFNALTGTSHSTDKYAPVDEPNVGVVQVI
                     DPRVTALSELYKPKKTIYANIEYHDYPGIFSSHAENADNALFSDIKSCEGFVLALRNF
                     PDDELDGLVGDYSPIRDLGNFEDEMILTDLIVAEKRLERIELGYKRGVRTPAIQLEER
                     VLKEVCEQLRDGKPIRGMEMAPEEAKTLRGFQFFSQKPLLVLLNCSEEGFGQQQGLLD
                     QIRSRGYLAIDIAGRFEEELSKLEDEEAAVFMEDMGITSSIRDRLTQLCYQLMGYLSF
                     FTVGEDEVRAWTITRGDNAVTAAGKIHSDLARGFIRAECFAYADLIDHGSEKALREKG
                     LFRLEGKEYIVKDGDIISIRFNV"
     gene            4827..7079
                     /locus_tag="PLO35_08025"
     CDS             4827..7079
                     /locus_tag="PLO35_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOH79657.1"
                     /translation="MAKTATAKKSTRKRGWKPWQRRLSAALIFIFCLLMVLGLLTGPQ
                     ALKDDMSVLKEAGGVLSWFSAAKTDASNPVGVFGILLGNLFVWLLGYLFSIVSFVIIG
                     IIALQYFLDPDAPKTRQKALLVLIVIFLLQILFSSHDRELGFAILPGSLWSVLAAIFS
                     SLGAAIILIAAMLLSLILIFEWRRVKEWALMLVESFERKGEERQLKKGKPTINQDEPL
                     PVRQSPGPEPVPVIQDHAQSTPPPPPEEAPAKKKAEPKPKKTSEEDDDEDREYVMPSI
                     GDFLESPVKLSERDRKEIESQILNTSGVLKAKLGEFGIEAEVRNVNIGPIITQYELEP
                     AKGVKVNRFTSLADDIALAIKAKSIRVQAPIPGRGLIGIEIPNLTRDMIYLRDLLLSE
                     EMRASKSKLMFGLGKDIAGKPVVTDMARMPHLLIAGATGSGKSVCINTIIMSLIMRTT
                     PDDLRLILIDPKRVELAGYNELPHLIGNVVTEPDNALENMYWAVREMERRYELLQEAR
                     VRDIAGYNEKVAIEKELELEPLPYIVIIVDEFADLIMTSGKDIELPITRLAQMARAVG
                     IHLILATQRPSIKVITGIIKANFPARIAFQVSSRVDSRVILDMIGAERLLGSGDMLFL
                     PPGKAIPERIHGAYVSDHEIARVCDFLAMQPKPKMDFSLPTKDDGEMGAIDYDDELFP
                     EAAAVVVRSNTASVSMLQRHFKIGYARAGRLIDLLERAHVIGPHLGSKSRDVLATRED
                     LVRMGLMRDE"
BASE COUNT         1622 a         2159 c         1965 g         1511 t
ORIGIN      
        1 tactctgagg cctctgtagt gaaaaaagac aactctgtat ctctgtagtg acgctgcagt
       61 gaggaaagcg cttatctggc cttggcgcgg ggttttttgg gcttgggagc gggcagcgcg
      121 tcggccgtca cgcgcaccag ttcagccagg aaaccaggct cttcccacat gtcttccggg
      181 atcaggaaat agtctttggc accgggatag ggtggcgcgg tctccacttc acccaggaaa
      241 gtttggccgg cttcggtggg tttgatgaaa agctggtcgt cgcagatcag ggccactatt
      301 ttgccgtcgc aatagaggcc gtattctcca aacattttga ggactttgct ctctccggcg
      361 ctgtggatct ggctgaggat gagctcagca gtggcggctt gtgtggccat ggcgtaaatt
      421 ccttcttatg gcagtttttg ggagtggagc atccgcgcag ggctgtaaac acctgcggat
      481 tatgctcata aagcacagaa ctatccccag cgataaccga agtgtatctg cttgtggacg
      541 gattatgcca gataaatctc agcgagacag ctttgtcctg gtcccccctg tagaggctct
      601 gcggaaggcc tttacctcga agcgatactt gccatcggag tagttgatct caaactcatc
      661 ttcacccact tggacgggtg tgagcagttt ggcatccttg cctttgccaa ccgtgtaggt
      721 gagggtgggc ccggagcttt ggcaggtgaa ggtgtaacgg cggtcctctc catcatcacc
      781 attctggaac agcccgcctt cctcgccttc ttcggccacg agagtgtagg ttccccacca
      841 ctcccagccg tcgctcgtgt catactcata gtcgtaagta ccgggattgc acaggtagaa
      901 cgttgtggta ttgaaaccga acggaatgcc ggggttgttg tcccaatatc tggtgcagtt
      961 gatgagccac actttcagaa aggtcttacc ttcctcgcca ttcttgccct tgcagccaaa
     1021 catcatcaac aggctcagaa gcaggagcag acaaataaga gctcttctca tgatcattac
     1081 ctccatgatc ctggattcac ttactcatgg agagcgcggg agttatgtaa gcgacctcca
     1141 caattcgcgg tcatttaaat gtaaatcata tgagtgtcaa gttaaatcgc agctggcctc
     1201 cgaaatgtca cacgctggcc ggcatatcct ggccaggggc caaactgggc ttgcgtgcac
     1261 gccatcgcgg tcctcggacg gtacaggact cagcccaggg ccagcagtcg cttgtattct
     1321 atcagtttat cccgtagaac aggatcgccg gtccccaata tctggatggc cagcagggcg
     1381 gcgtttttgg caccgttgat ggccacgcag gctactggca ctccgggagg catctgggcc
     1441 acgctgtaga gggcatccac gccgttcaaa gctcctcccg ccagggggac tccgatcacg
     1501 ggcagatggg tgtgggaagc caccactcct ggcaggtgag ccgccatgcc agcgcaggcg
     1561 atgatcactt tcacgccttc ggaggcggcg tttttggcca gcagagcggt ctgctccggt
     1621 ttgcgatggg cggaggagac gtggaaatca taatccacct caaactccgc cagggtgtcc
     1681 ttaatcggct ggcagctctc cagatcggac ttgctgccca ggatcactct cacctggggc
     1741 attatctgtt ttgggtttcg ggaagcaccg tgagggcttc gtggatggct tccttatcgg
     1801 cggcactgct gaagctggcc aggatctcgg gattcatgat cagcttggag atgtagctga
     1861 ggctgaaaag gtgctgtttg tcgttgtgca gcagcaacat aaagaagatg ttgacgggct
     1921 tgttgtccgg cgcgtcaaaa tccactccct caaggctgcg gccaaagagg atggaaggca
     1981 ccttgatctt ggagggatgc aggtgacggg gatgcagcag ggccacgcca ttgccgatag
     2041 ccgtggagat cagttcctcg cgggccacca ccacttcata gagccagcgg gcgtcgcgca
     2101 ccagcttgag gtccttggcc ttttcgctca gcacccggat ggcgtcgtat ttgttttcgg
     2161 cggcaaagtc caggaagatg ttttcggggc gcatgtattc ctcaaagtgg cagatcacgc
     2221 gcttcagggc cagcatcttc tcttcgcttt cgttgaggtt ttccagccat tcgttgaggg
     2281 agtcttcgtc gatcttgacg ttctttccca ccagcttgct ctcgaagacc ttgctgttga
     2341 tcatctcctg gatgaccttt tcggacactt tgagcttctt ggccgcgtcc gctcgggaca
     2401 ggaatttttt ggccatggtg atacctcctt tgagtatcgg ggacatcaat gtttttatct
     2461 gaattcaagg tttatcttga caaggatatg attttttttg ggcttgacat atggtcaaga
     2521 aaataattga agcagccctg ctctgcgttg tactttttgg atgctcatat tctgtgtatt
     2581 caaacgctta tccgcatcta aaaaaaattc gcgtggcccc ctttgagaat cgctccgcgg
     2641 agtttgatat cgccgatcag gtgttcaccg gactgaaccg gcagtttaga aacgatggca
     2701 ggctcaagcc tgtcactcag gccccggact gccagttgga aggcgtgatt accagtttcg
     2761 aagagcgcgt ttacagctac gacaccggca acaacatcca ggattatcag ctgcggctca
     2821 cgctcagcgt caccttctca gacctcgtgc gcaacgagat catctgggaa aacaaaagcc
     2881 tcacgctctc ggagacttat gccgtcagcg aggccagcac cgccaaattt aaaagcaaag
     2941 aggaagccac cgatgagctc atccagagtc tcttccggac gatcgtccaa aacagcctcg
     3001 aacgctggtg aaggcattcg cctcaaccgc tttctggctg aaaacggcct cggttcacgc
     3061 cgtaaaaccg aagagctgat cagctccggc cgggtcagca tcaacggcca gaccgtcacc
     3121 gatctgggac gccgcgtcaa ccccctccag gatgaggtca aggtggacgg caaagccctg
     3181 ggaatcacca aagagctgct ctatctggtg ctcaacaaac cccgcggcta cgtggtctcc
     3241 catgccgatg aacaaggccg ggacacgatt tacaagctgc tgcccgtcga ggcagccaac
     3301 ctcaattatg ccggccgtct ggacaaaaac tccgaaggcc tcctgctgct cactaacgac
     3361 ggcgatatga tcaacctgct cacccatccc aatttcaagg tggaaaaggt ctacaaggtg
     3421 gatgtgacgc cccgcctctc cagagcggcc atcactcagc tccgggaagg agtggccatc
     3481 gaaggtggaa tgaccctttc agccggagtt tacgtgaaaa gcgaacacga gggcggcatg
     3541 accctcaagg tcgtgatccg cgaaggccgc aagcgccaga tccgtcagat ggtggaagcc
     3601 gtgggcggca aggtcaaggg cctcaagcgc ctccagtttg gccccctgat gctcaaaaac
     3661 ctggccaccg gagcctggcg tcccctcaac cccatcgagc tcaaagccct ccgctacatc
     3721 tgcgacaaag gaaagaaact gcatgaagat cgccctcatc ggcctgccca aaagcggtaa
     3781 gaccaccatt ttcaacgccc tcaccggcac ttcccactcc acggacaaat acgctccggt
     3841 ggacgaaccc aacgtgggcg tggtgcaggt gatcgatccc cgcgtaactg ccctctccga
     3901 gctctacaag cccaagaaaa ccatctacgc caatatcgaa tatcacgact accccggcat
     3961 cttctccagc catgccgaga atgccgataa tgccctattc tcagacatca aaagctgcga
     4021 aggctttgtg ttggccctca gaaacttccc ggacgacgag ttggacggcc tggtgggaga
     4081 ttacagcccc atccgcgacc tgggcaactt tgaagacgag atgatcctca ccgatctgat
     4141 cgtggcggaa aaacggctgg agcgcatcga gctgggctac aagcgcggcg tcaggactcc
     4201 cgccatccaa ctggaggaga gggtgctcaa agaggtctgc gagcagctgc gagacggcaa
     4261 gcccatccgc ggcatggaga tggcccccga agaggccaag accctgcgcg gctttcagtt
     4321 cttcagccag aagcccctcc tggtgctgct caactgctcc gaagagggct ttggccagca
     4381 acagggcctc ctggaccaga ttcgctcccg gggctatctg gctatcgata tcgccggccg
     4441 ctttgaagag gaactcagca agcttgagga tgaggaagcc gctgtcttta tggaagatat
     4501 gggcatcacc agctccatcc gcgaccgcct cactcagctc tgttatcagc tcatgggcta
     4561 cctcagcttt ttcaccgtgg gagaggacga agtgcgcgcc tggaccatca ccagaggtga
     4621 caacgccgtc accgcggccg gaaagatcca cagcgatctg gcccggggct tcatccgcgc
     4681 cgaatgcttt gcctacgccg atctgatcga ccatggcagc gaaaaagccc tgcgcgagaa
     4741 aggcctcttc cgtctggaag gcaaggaata catcgtgaag gacggagaca tcatctccat
     4801 ccgtttcaac gtctaaggaa tattttatgg ccaaaaccgc cactgccaaa aagagcaccc
     4861 gcaagcgagg ctggaagccc tggcagcgtc gtctgtccgc ggccctgatc ttcatctttt
     4921 gcctgctgat ggtgctggga ctgctcaccg gtccccaggc cctcaaggat gacatgtccg
     4981 tattgaaaga agcgggtggg gtcttgagct ggttttccgc cgccaagacc gacgccagca
     5041 atcccgtggg cgtgtttggc attttgctgg gcaacctgtt tgtctggctg ctgggctatc
     5101 tcttttccat cgtgagcttt gtgatcatcg ggatcatcgc gctgcaatac tttctggatc
     5161 cggacgctcc caagacccgt cagaaggccc tgctcgtcct gatcgtgatc ttcctgctgc
     5221 aaatcctgtt ttcctctcat gaccgcgagc tgggctttgc catcctgcca ggctccctgt
     5281 ggagcgttct ggccgccatc ttcagttcct tgggcgccgc catcatcctg atcgccgcca
     5341 tgctgctcag cctcatcctc atctttgaat ggcgccgcgt caaagaatgg gccctgatgc
     5401 tggtggagag cttcgagcgc aaaggcgaag agcgccagct caaaaaaggc aagcccacca
     5461 tcaatcagga cgagcccctg cccgtgagac aatctccggg gccggaaccc gtccccgtga
     5521 tccaggacca cgctcagagc actcctccac cccctcctga agaagccccg gccaaaaaga
     5581 aagcggaacc caagcccaaa aagacttccg aggaggacga cgacgaggac cgcgaatacg
     5641 tgatgccctc catcggagac tttctggaga gccccgtcaa gctctcggag cgcgaccgca
     5701 aggagatcga gagccagatc ctcaacacca gcggcgtgct caaagccaag ctgggcgagt
     5761 ttggcatcga agcggaagtc cgcaacgtca atatcggccc catcatcacg caatacgagc
     5821 tcgaacccgc caaaggcgtg aaggtcaacc gcttcacctc cctggcagat gatatcgccc
     5881 tggccatcaa agccaagagc atcagggtcc aggcccccat accgggacgc ggtctgatcg
     5941 gcatcgaaat ccccaacctc acccgcgata tgatctatct gcgcgacctg ctgctctccg
     6001 aagaaatgcg cgccagcaaa tccaagctca tgtttggcct cggcaaagac atcgccggaa
     6061 aacctgtggt caccgacatg gccaggatgc cccacctcct catcgcgggc gccaccggaa
     6121 gcggtaaaag cgtgtgtatc aacacgatca tcatgagcct catcatgcgc accactccag
     6181 acgacctgcg cctcatcctc atcgatccca aacgcgtgga actggccggc tacaacgagc
     6241 ttccccacct gatcggcaac gtagtcaccg aaccggacaa cgccctggaa aacatgtatt
     6301 gggccgtgcg cgagatggag cgccgctacg aactcctgca ggaagcccgc gtgcgtgaca
     6361 tcgccggcta caacgaaaag gtggccatcg agaaggaact ggagttggaa cccctgccct
     6421 atatcgtgat catcgtggac gaatttgccg acctcatcat gaccagcggc aaggatatcg
     6481 agctgcccat cacgcgtctg gctcaaatgg cccgcgccgt gggcatccac ctcatcctgg
     6541 ccacccagag gccctccatc aaagtcatca ccggtatcat caaggccaac ttccccgccc
     6601 gcatcgcctt ccaggtttcc tcccgggtgg attccagagt gattctggac atgatcggag
     6661 cggaacgcct gctgggaagc ggagatatgc tctttctgcc ccccggcaag gccatcccgg
     6721 aacgcatcca tggagcttat gtcagcgatc acgaaatcgc ccgggtctgc gacttcctgg
     6781 ccatgcaacc caaacctaag atggacttta gtttacccac caaagacgac ggcgaaatgg
     6841 gcgctatcga ctatgacgac gagctctttc cagaagcggc cgcggtggtg gtaagatcaa
     6901 acaccgcttc cgtttccatg ctccagcgcc acttcaaaat cggctacgcc agagccggcc
     6961 gcctcattga cctccttgaa cgcgcccacg tgatcggccc ccacctcggc tccaaatcca
     7021 gagacgtgct cgccacccgt gaagacctgg tccgcatggg actgatgcgc gatgagtgag
     7081 attatccttt caacccagag aaaagcagag cttgagcaga gaaaagcaga ggatttatct
     7141 tcacaatgag tattacagat aaactatgag atatgagacc atgaaaaacg ctgtaacgct
     7201 ggctttagcc ggccgtagca ctgactttag tcggtgcgtg accacatccc gttggag
//
LOCUS       DAOSFN010000128         7206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_9010_length_7206_cov_9.670815, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000128 DAOSFN010000000
VERSION     DAOSFN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9010_length_7206_cov_9.670815"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..>1784)
                     /locus_tag="PLO35_08030"
     CDS             complement(3..>1784)
                     /locus_tag="PLO35_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79658.1"
                     /translation="LSPFVVSVSDSCPNGAVLSFSCEISYGGSSQSFPLSLTVRRPAV
                     EFGGYLINDADGDGNGILEPGDSAAFVVNFKNTGYIEARNFVATLACSNPLISLLDTH
                     IQIASVPPGTTAQASFRFTLSSSAALGSNVNVNLSYGCDLMTGLTSQIQARIGSSGLY
                     DDFESSFGSYVSIPSSSAWQWGTDQTAGAHSGSKVWGTALNQQYPNDVTWKLSSPSIY
                     IGPHNYLEYWQCYDTQSGCDGGQLEIYAGGTWTLLTPIGGYPTANVAALNGPGFSGSS
                     AWSPVRCDLSPYANGNVRFRWTFASDASVQGQGWYIDDVRTIGFTNLGCKLSGSIALD
                     GPLPDQSEVWIGKAAGINVNPLPDLSYTLYLPIGIHSVSCSAPGYSIDTAIDLDFSLD
                     TPSLQHDFVVHELKPVTDLTSSHTASVLTLHWEAPSEPYFPVTGYKVVRRINAGAFET
                     AVVTSEPVYTELMEVPGQYRYYIVALYEEGESLPSELLSIGFPFPDEPPLPLITRLFA
                     NYPNPFNPYTTISFDLAASQSAALRIYNLRGQLVNTLCDSELSAGSYSYIWDGRDAGR
                     SRVASGVYFYRLSTASHTETRKMLLLK"
     assembly_gap    1785..1884
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1885..5231)
                     /locus_tag="PLO35_08035"
     CDS             complement(<1885..5231)
                     /locus_tag="PLO35_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH79659.1"
                     /translation="MLLTLGGLGASLSDEAHPVRIAECGPERIVMEFICPQARLESVT
                     VNGERYDSVSLQGAELSAEEGRPDLPQFGSMVAIPPNSVYSISYELSEPQIFEDVKPL
                     PVRDASRSLMLSPDYYESAPYPSRQVIAGEAVYLRDFRVLPLQTCPFSWEPSTNTLTR
                     YGHIRINIDLSYPKGDSGSIPYTGYSYAFKNLYEARIQNFSAYRDLESGPQNARVLII
                     HGHNTDPVFISKLDEFVTWKRQKGFIVSVASTQVAGTTNSAIKSYIQAQYDDPGTRPD
                     FIILIGDVAGSCAVPTFFETESEFNAEGDYPYTYLSGDDMLGDAFIGRLSAEDVSQLL
                     TLYNKVYTYEKTVNINPPASDWMNRMLVIGDPTTSGISCAYTARFIKEIAQRANPDYS
                     FVENYTSGFVSTINSGLNQGVGFFAYRGYAGVSGWSPSTSVVNGFKLPHAVILTCQTG
                     SFAGTSLSEQIIRMGTESVPAGAATCIAMASPGTHTSFNNALISGIMSGVFCHGMRTM
                     GEALLNGRLYLHEIYGATQPAFVRSLAHWCNLMGDPTLETWVGVAGELNIEVQAPLPE
                     GLSILEVVVRDSLQLPVEGVSVTAWSPSQNAVVASAFTMANGIAVLELPASPGPELLI
                     TAAKHNFKPCQQTLSVESTGSLVYAAHQVIDDGSLGSSGNANGTVNASETIALNVQIK
                     NTTASELSSLSAVLSIDHPGIQITQGTATYPVLGPGMTGYGSQPFILSINSSISPLET
                     VRCLVNVTDDEANVYQSVFYLRASNSRLSISGWTVNEPGNGVLDPGEECSLNLELSNA
                     ADYPAPDLFGELHSLNSLLAVSDSIAYFGTVTGQSQTVSVDGFQVKASSDLVAGMQLP
                     MRARFYNSAGFEQTCLFNLSVGTVSQTTPLGPDEYGYLIYDETDTDYPDCPVYDWVEI
                     VPSLGGRGTLIPNLGDTGAGTSEGETTTATVLKELMLPFAFRFYGIDYDRITVCVNGF
                     IALGSTNNGEFRNARLPGGQGPCPMIAAFWDDLTLPLSAGLYQYYDEAGHCFIIQYNH
                     LENGYDDSSDATFQVIFYDPVYHHSGLGDGIIKLQYKTFNNIDAGGTAGYYDTPLHGN
                     FCTVGIKDHTNTRGLEYTFNNTYPQAAA"
     gene            5811..6254
                     /locus_tag="PLO35_08040"
     CDS             5811..6254
                     /locus_tag="PLO35_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MraZ"
                     /protein_id="HOH79660.1"
                     /translation="MSGQFIGYFENAVHKQRVIIPAAFKKKFSEEARRTVVVTLGPLN
                     TIAIFPLDCWNSFLDKLAGGDDDDQELRTQLIDFAVTEQELEGPGRIRIPEQLLAEAN
                     IGDSVIIKGELHYISLWDPAEFHAHRRAKKDAHRKKFTTRHYQKS"
     gene            6251..>7206
                     /gene="rsmH"
                     /locus_tag="PLO35_08045"
     CDS             6251..>7206
                     /gene="rsmH"
                     /locus_tag="PLO35_08045"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOH79661.1"
                     /translation="MSVYHTPVMATECVELLQLRPGGVYIDATLGAGGHALAILKAVS
                     GTRLFGFDRDDEAIAAARQNFAAYALAASDKREADFRDRIEYIHAPFSSLRAQLALRK
                     VSGIDGILFDLGVSSHQIDSASRGFSFDKDASLDMRMDARQALTAAKIVNGSTVKELA
                     TIFKLYGEEQQAGRIARAIDQARSSQPLARTSQLARVIESVAGTGTRDSLKTKMRVFQ
                     ALRIAVNSELRELETALKDAIMLLKPGARVVVLSYHSLEDRIVKTLFREAAKGCLCPP
                     RQIVCSCGLKPRLKVLTGRPLSASEAEISANPRARSAKLRAA"
BASE COUNT         1689 a         1867 c         1955 g         1595 t
ORIGIN      
        1 tactatttca gcagcagcat cttgcgggtt tcggtgtggg acgcggtgct gaggcggtaa
       61 aaatacactc ctgaagccac tctgcttctg ccagcatctc tgccatccca tatatagctg
      121 taagagccgg cgcttagttc gctgtcgcag agagtgttga ccagttggcc ccgcagattg
      181 taaatcctaa gagcagctga ttgggaagcg gcgaggtcaa aactgatcgt agtgtatgga
      241 ttgaaaggat ttgggtagtt cgcgaacagc ctggttatca gaggcaaggg tggctcatcc
      301 ggaaagggaa agcctatgct gagcagctcg gaaggcaggc tctccccctc ttcatagagg
      361 gcaactatat aataacgata ctggccagga acctccatca attcggtata tacgggctcg
      421 ctggtcacga ccgccgtttc gaaagccccg gcattgatcc tgcgaacgac tttatatccc
      481 gtaaccggga aatagggttc cgagggagct tcccaatgga gggtgaggac gctggcagtg
      541 tggctcgaag tcaggtctgt aacaggtttc agctcgtgga ccacaaaatc atgctgcagg
      601 gagggtgtat cgagactaaa atccagatca atggcagtat cgatgctgta accaggcgcg
      661 gagcaactca cactgtggat gccaatcggc agataaagcg tgtaactcaa atctggcaaa
      721 ggattaacgt tgattccggc cgctttgcca atccacactt cggattgatc tggcagcggc
      781 ccgtccaacg cgatcgagcc actaagttta caccctaaat tggtaaaccc gatggttcgg
      841 acgtcgtcga tataccatcc ctggccctga acagaagcgt ctgaagcaaa ggtccagcgg
      901 aagcggacat tgccgttggc gtatgggctt aagtcgcatc tgacgggact ccaggctgag
      961 cttcctgaaa atcccggacc gttgagggcg gcaacattgg cagtgggata tccgcctatg
     1021 ggagtcagca gagtccaggt tccccccgcg tagatttcca gttggcctcc gtcgcagccg
     1081 ctttgagtgt catagcattg ccagtattcc agatagttgt gggggcctat atatatggaa
     1141 ggggagctga gtttccaggt cacgtcgttg ggatattgct ggttgagcgc ggtaccccag
     1201 actttgctgc cggaatgggc cccggcagtc tgatcggtgc cccattgcca agcggaactg
     1261 ctcggaatgg acacatagct gccgaaactg ctctcaaaat cgtcatacaa tccgctgctg
     1321 ccaattctag cctggatttg ggaggtcaag cccgtcatca ggtcacaacc ataactgaga
     1381 tttacgttta cgtttgagcc caaggccgct gaggaggaaa gtgtgaagcg aaatgaggct
     1441 tgagcggtag tccctggcgg aacgctggcg atttgtatat gggtatccag caagctgatg
     1501 agcggattgg agcaagccag cgtcgcgacg aagtttctgg cctcaatata accggtgttt
     1561 ttgaagttga ccacgaatgc ggcgctgtct ccaggctcca ggatgccgtt cccatcgcca
     1621 tccgcgtcgt tgatcagata tccaccaaat tccacagccg gacgcctgac agtcaatgac
     1681 aatggaaatg attggctgga ccctccatag ctgatttcac aactgaagct caaaacagcg
     1741 ccattgggac aggagtctga aacgctcacc acaaaagggc tcagnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnngccgcg gcttgcggat aggtgttgtt gaaagtatat
     1921 tccaggcccc gggtgttggt atggtctttg atcccgacgg tgcaaaaatt gccgtgcagg
     1981 ggtgtgtcgt agtatccggc tgtgccgccc gcgtcaatgt tgttaaaggt cttgtactgg
     2041 agcttaatga tcccgtcgcc aaggccgctg tgatggtaaa cgggatcata aaaaattacc
     2101 tgaaaggtgg cgtcggagct gtcgtcataa ccgttttcga ggtggttgta ctggatgata
     2161 aaacaatgtc cggcctcatc gtagtattgg tagaggccag cagacaaagg cagagtaagg
     2221 tcatcccaaa aagccgcgat catggggcag ggaccttgcc cacccggaag gcgcgcattg
     2281 cgaaattctc cgttgttggt gctgccgaga gctatgaatc cgttcacgca gactgtgatg
     2341 cggtcataat cgataccata aaagcggaag gcgaaaggca acatcagttc tttcaggact
     2401 gtggcggtgg ttgtctcacc ctctgacgta ccggctcccg tgtctcccag gttcggtatc
     2461 aaggtccctc ttcctcccag actgggaacg atttccaccc agtcatagac agggcagtcg
     2521 ggatagtcgg tgtcggtttc gtcatagatc agatagccat attcgtccgg ccccagaggc
     2581 gtggtttgtg agacggtgcc gacgctgagg ttgaaaaggc aggtctgctc aaagccagcc
     2641 gagttgtaaa aacgggcccg cattggcagt tgcatccctg ccaccagatc actgcttgcc
     2701 ttcacctgaa aaccgtccac cgagaccgtt tgcgattgcc ccgtcacagt tccaaaatat
     2761 gctattgaat ccgataccgc gagcaggcta ttgagagaat gcagttcccc aaagaggtcc
     2821 ggagcgggat aatccgccgc gttgctaagc tccagattga gggaacactc ctctccggga
     2881 tcgaggaccc cattgcctgg ttcgttcacg gtccagccgc tgatactcag ccgcgagttg
     2941 ctggcccgca ggtagaatac cgattgatac acattcgcct cgtcgtccgt gacgttgacg
     3001 aggcagcgaa ccgtctccag gggcgaaatt gatgaattga tggaaaggat gaagggctga
     3061 gatccataac ctgtcattcc cggacccaga acaggatatg ttgccgtccc ctgagttatt
     3121 tggatgccgg gatggtctat gctgaggacg gcactcagac tgctcagctc agacgcggtt
     3181 gtattcttga tctgcacgtt gagggctatg gtctcagagg cattgacggt cccgttcgcg
     3241 tttccggaag agcccaggct gccatcgtcg atcacctggt gggcagcata gaccaaagat
     3301 ccggtgcttt cgacgctgag ggtttgctga cagggcttaa agttgtgttt cgcggctgtg
     3361 atcagaagtt cagggcctgg agacgctggc agttccaata cggcaatgcc gttggccatg
     3421 gtgaaagctg aagctaccac ggcattctgg gaagggctcc aggccgtcac gctgactccc
     3481 tccacgggaa gctgcagact gtctctgact actacttcca ggatgctcaa tccctctgga
     3541 agcggggcct ggacctcgat gttgagctct cctgccacgc ctacccaggt ctccagagtg
     3601 ggatctccca tcagattgca ccaatgggcc agtgatctga caaaagctgg ctgggtggcc
     3661 ccataaatct catgtagata aagccttcca ttgagcagcg cctctcccat cgtgcgcatg
     3721 ccatggcaaa aaacgccgct cataatcccg gagataaggg cattgttaaa ggatgtatgg
     3781 gtcccggggg aagccatagc gatacatgtt gccgccccgg cggggacaga ttccgttccc
     3841 atcctgataa tctgctccga taaagaagtt cccgcgaaac tgcccgtctg gcaagtaagg
     3901 ataaccgcgt ggggaagctt gaaaccgttg acgaccgagg ttgagggcga ccaaccgctt
     3961 accccggcgt agcctctgta ggcaaaaaat cccactccct gatttagacc gctgttgatg
     4021 gtgctgacaa aaccacttgt gtagttttcc acaaagctat aatcaggatt ggctcgttgg
     4081 gcgatctctt tgatgaaacg agcggtataa gcgcaggata ttcctgaagt agtgggatct
     4141 ccgatcacca gcatccgatt catccagtct gaggcgggag ggtttatatt gacggtcttt
     4201 tcataggtat ataccttatt atatagggtc agcaactggg agacgtcttc cgcggacaat
     4261 ctaccaataa aggcgtcacc cagcatatcg tcgccgctca gataagtgta gggataatcc
     4321 ccttcagcgt tgaattctga ttccgtttca aagaaagtgg gcaccgcgca gctgcctgcc
     4381 acatccccga tcaggataat gaaatcggga cgcgtccccg gatcgtcata ctgggcttgg
     4441 atatagctct tgatggccga attggtggtg ccggccacct gtgtgctggc tacgctcaca
     4501 ataaagccct tctgacgctt ccaggttacg aactcgtcca gcttggagat gaagaccgga
     4561 tcggtgttgt ggccgtgaat gatcagcacg cgagcgttct gaggtccgga ctccagatcg
     4621 cgataagcgc tgaagttctg gattctggcc tcatagagat tcttgaaggc atagctgtaa
     4681 ccggtgtagg gtatggagcc cgaatcccct tttggatagc ttaggtcgat gttgatcctg
     4741 atgtgaccgt aacgggtgag ggtgtttgta gagggctccc aggaaaatgg gcaggtttgc
     4801 agtggaagca ctctgaaatc tctcagatac accgcttcac ctgctataac ctgtctggag
     4861 ggataagggg cagattcata atagtctgga gatagcatga gagacctgga tgcatcccgc
     4921 accggaaggg gttttacatc ctcaaatatc tggggttcag atagttcata gctgatacta
     4981 taaactgagt ttggcgggat cgctaccatg ctcccaaatt gtggaagatc gggtcttccc
     5041 tcttccgcgg ataattcggc cccctgcaga ctcacgctgt catagcgctc gccgttcaca
     5101 gttacgctct ccagccgggc ctggggacaa atgaattcca tgactattct ttcgggaccg
     5161 cattccgcga tcctgacagg atgggcctcg tcagatagcg aggcacccaa gcctcctaat
     5221 gttaatagca tcaacatgct aacaataatc ctgtcacctt tcatctgctg tacctcttga
     5281 taaaatctca ggtccattct cctgatcaaa tccccgggtt ggggtcacat cttggttcac
     5341 ggtcttgatg agcatccgga gtttgattga gtcctagcct gacaaatact caccattcct
     5401 gtccagcgca caataaatga aaccaagacc ttgtgtcaag caaaatcttc agctctggcg
     5461 atactgacaa taaagtattc aaattaaaca ggatcattta cataactcct tgctgagagt
     5521 taattcaact agtccggcac tttttttcaa ctccgttcct gcagactcag aatcaagccg
     5581 catcaaggtt ttcggccaca tccagtaagc tgttgctcag attctccagg aaaaaatcaa
     5641 aaaaaggtca ttttgttctt gacaagtatc acctcccgtc ataaaagcac cacaggatgt
     5701 cacaaaaagt cagaaaatgt caaaccggga gtacaggaat gccagttaaa ttcatgctaa
     5761 ttaacaatat atacaggtcc agcactaaag cttaggaaaa ggtcgtcagc atgtccggac
     5821 aatttatcgg ttacttcgaa aacgctgttc acaagcagcg ggtgatcatt ccggccgcat
     5881 tcaagaagaa attctcagaa gaagcgcggc gtacggtggt cgtcaccctc ggccccctga
     5941 acacgattgc catctttccc ctggattgtt ggaacagctt tctggacaag ctggccggcg
     6001 gcgacgatga cgaccaggaa ctccgcaccc agctcatcga cttcgcggta acggagcagg
     6061 agctcgaagg tcctggccgc atccgcattc cagagcaact gctggcagag gccaatatcg
     6121 gggacagcgt catcataaaa ggcgagctgc actatatatc tctgtgggac cccgcggaat
     6181 ttcacgccca ccgtcgggcc aaaaaagacg cccaccgcaa aaaattcacc accaggcatt
     6241 atcagaaatc atgagcgtct atcacactcc cgtcatggcc acggaatgcg tggagttact
     6301 ccagctccgg ccgggcggag tgtatataga cgccactttg ggcgcgggag gacacgctct
     6361 ggccatcctg aaggcagtct ctggcaccag gctgtttggc tttgaccggg atgacgaggc
     6421 catcgcggcg gcgcggcaaa acttcgcggc ttacgcgctc gccgcctctg acaagcggga
     6481 ggcggacttc cgggatagga tagagtatat tcacgcgccg ttttccagct tgcgcgccca
     6541 attggccctc cgcaaagtga gtggtatcga tggcatcctc tttgatctgg gagtatcgtc
     6601 tcatcagatc gactccgcca gccgcggttt cagttttgac aaagacgcca gcctggacat
     6661 gcggatggac gcccgtcaag ctctcaccgc cgccaaaatc gtgaacggca gcaccgtgaa
     6721 ggaactggcc accatcttca agctctatgg agaggaacaa caggccggca gaatcgccag
     6781 ggcgattgac caggccaggt cctcccagcc gctggcccgc acttcccaac tggccagagt
     6841 gattgaaagc gtggcaggta ctggaaccag agattcactc aagaccaaga tgcgggtgtt
     6901 tcaagccctg cggattgcgg tgaacagcga actgagggag ctggaaaccg ctctcaaaga
     6961 cgcgatcatg ctgctgaagc ccggggcccg ggtggtggtg ctgagttatc actccctgga
     7021 agacagaatt gtaaagactt tatttagaga agccgccaag ggctgtttgt gccctccccg
     7081 gcagatagtg tgcagttgcg gtctcaagcc gcgcctgaag gtcctcacgg ggcgcccgct
     7141 gagcgcttcg gaggctgaga tttcagccaa tccgcgcgcg cgcagcgcta aactaagggc
     7201 cgcgga
//
LOCUS       DAOSFN010000129         7203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_9019_length_7203_cov_10.100028, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000129 DAOSFN010000000
VERSION     DAOSFN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9019_length_7203_cov_10.100028"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1266)
                     /locus_tag="PLO35_08050"
     CDS             complement(250..1266)
                     /locus_tag="PLO35_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HOH79662.1"
                     /translation="MKAYLALVICLLAFPILAQSNDIDALAREFFRLNTPEASLKADF
                     LDQIDMIAPYLGSMMKEEGLSAGSDFSMEGLKELFGFILHELDYDELERIGAGIISSQ
                     YSLDELIAINAFLKSSAGQAYIYNSRPGSNYLTQTMKGYFEEKAQDETWLMGLIMSLL
                     ANIGLEDGLETAPAPEEETWPTPAVADSSDYYGWDDYWDTPAPIDSSYWFEETPAPAD
                     TAAVSYDSWEDYWAGPPAITITMNGRFFERMNEIGYLYSELMPVMAQVYKWLDDYTHE
                     YGGYPNELDWTQIDTVNGYDLSYDHDNALLKAVSNSSYKLPGIELIYYLNENSVWISV
                     PSQR"
     gene            1777..2874
                     /locus_tag="PLO35_08055"
     CDS             1777..2874
                     /locus_tag="PLO35_08055"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOH79663.1"
                     /translation="MKKRLLIATGGGDCPGLNAVIRAIVKRSSHEHNWEILGSIDAFN
                     GLLLDPMHLEELTLQRVAGIHVKGGTIIGTTNKGGPFEWPVKNSDGTWSTVDRSKDFM
                     DRLRYQNIEAVINIGGDGSQRISQKLFELGCPIIGVPKTIDNDLSDTDFTFGFQTAVD
                     AATDAVDKLVSTAASHHRVLILEVMGRYAGWIALHAAIAGGAEVCLIPEIPYDLKKVI
                     EVIDQRVDNGKGFAIIVIAEGAKPIEGGMSYSENDTPGAMKIRLGGAGLRLSHDLKEA
                     GSCVEIRETILGHLQRGGRPNSFDRILASMFGVKAFELVLEEKWGHMVAYRHPNVVAV
                     PISDAIKEYNLVDVNSDLVKTARGLGISLGD"
     gene            complement(2991..3746)
                     /locus_tag="PLO35_08060"
     CDS             complement(2991..3746)
                     /locus_tag="PLO35_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOH79664.1"
                     /translation="MDDRVLTLKARLQALLESRDDVVAAWEGGSAATGYLDEHSDLDL
                     LVVTKQGSSAEPVFEAMNELFESQWGVLESFRMPEPAWHGLSQCFYRLDKLPPLFYCD
                     IAVTGEENPNKLTEPERHGNAVIWFDKSAIYSSKPMPPEELKQLRDRMYAMATGTWWL
                     MLIELKKALARENWLSALGNYNALLGRHVVSLMNLKYRPWKADFGSRYAERDYPSEAA
                     DELIWLTQVASLEDIRERLPRLEALYAQLVEEL"
     gene            complement(3759..4064)
                     /locus_tag="PLO35_08065"
     CDS             complement(3759..4064)
                     /locus_tag="PLO35_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOH79665.1"
                     /translation="MNSSTQAILDHIGAIPPGRVMGYARVAEAAGIAHGARLVVRILH
                     TQSDSQDLPWWRVVRSDGSIGLSEECGGALQRSMLEAEGVVFDASGKIDLERYGYNG"
     gene            complement(4072..4707)
                     /locus_tag="PLO35_08070"
     CDS             complement(4072..4707)
                     /locus_tag="PLO35_08070"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOH79666.1"
                     /translation="MDYTQLRAEARDLLRGRWNTCALLMFIVSVLSGSVSLIFGLGNT
                     QFLSSPLSILVGAPLNLGVTAIFLKLYEGHNFQIEEVFNGFRDFSRAVISGLLISVYV
                     ILWSFLLIVPGIIAALGYSMTFFILVENPQLSASQAMALSKEMMLGHKTELFMLSLSF
                     IGWFFLSLFTFGIGIFWLAPYVDMSYTIFYHRVKAHGGVVNPGETVTGIIR"
     gene            4865..6037
                     /locus_tag="PLO35_08075"
     CDS             4865..6037
                     /locus_tag="PLO35_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79667.1"
                     /translation="MKHYCLMVISFLCLFTALGAASPQYRVLLEWDEMEGELNGILSG
                     IVDSRYGFIQGAGPGQVLDGAISSRGENTMRGASQEFYINIADGYYSFWIRDKFADDD
                     INPDPNLIRAAKPRVSVYRGNALVGRVDITQGAGLTCKVFTLDASNGAFDQEIRFFPR
                     ARIFAVMIVDAINGNGLPNVQISITGGEDRYPSVTTDASGFAYIPVEIGSYRLEMYKE
                     GYINTSFLVSMGFDENPREFVFAIAPKAKDYRIVLTWGSRPNDLDAHLTGPKPEGGDF
                     HIWYRNRILIAGRDFLDRDDTDKYGPETVTIYKPASGEYSFCVHDYSNRSDRSSRMLS
                     FSNAVVQVYADDALKATFTVPEGQFGNLWRVFKIDKNQRVVSVNTVGWTDDEKNIR"
     gene            6057..6623
                     /locus_tag="PLO35_08080"
     CDS             6057..6623
                     /locus_tag="PLO35_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79668.1"
                     /translation="MKSKTMLFVILAVVLLVALTACSKDKKTPAPQAEGEVVNFPAWW
                     NTQNDDAYVCTYGIGTNVKMTTSMDTAKNNALFEAAQYVEVEVKGMLKTYEEEAGVFD
                     PQLLALTSKVIKTVADATFSGVINGQTETRKVTEFGGPRYTTYLQLKIPKTEVKKTLY
                     NNIRNEEALYNQFKASMAFEELERTVGQ"
BASE COUNT         1705 a         2017 c         1875 g         1606 t
ORIGIN      
        1 tgtaactggt gagctgctgg ctcattctcc aggcccggta accgagctgg gagctattga
       61 aatcaaatac tccgcgaccg tcgaagacgc cagccgaaag caacatgggc aatagcacca
      121 gcatcaaagg cacgtaaaac tttctcatca tacacctcgt gttttgtatt tatgctccca
      181 gcttgtgggc tggggattcc gtgtcaataa aaatcgcttc cggataacaa agatcaaggc
      241 cgggatggat caccgctgag aggggaccga gatccagacg ctgttttcgt tcaaataata
      301 gatcagttct atccccggca atttatagct ggaattgcta acggccttga gcagagcgtt
      361 gtcatggtca taggacagat catagccatt gaccgtgtcg atctgagtcc aatccagctc
      421 attcggatag ccgccatact catgcgtgta gtcgtccagc cacttataga cctgcgccat
      481 cactggcatg agctcgctgt acagataccc gatctcattc atgcgctcaa agaaacggcc
      541 gttcatagtg atggtaatgg ccggaggacc tgcccaatag tcctcccagc tatcataact
      601 cacggccgcg gtgtccgctg gggcgggtgt ttcctcgaac cagtaagatg aatcgatcgg
      661 tgccggcgta tcccagtaat catcccaacc gtaatagtcg gatgagtccg ccactgcagg
      721 agtgggccag gtttcttcct ctggagcggg ggcagtttcc aggccgtctt ccaggccgat
      781 gtttgccagc agggacatga tcagccccat cagccaggtt tcgtcttgcg ctttctcttc
      841 aaagtagcct ttcatggtct gggtcagata gtttgagccg ggccgcgagt tatatatata
      901 ggcctggccg gcggagcttt taaggaaagc gttgatcgcg atcaattcat ctaaactgta
      961 ctgcgagctg atgattcccg ctccgatccg ctccagttca tcataatcca gctcatgcaa
     1021 aatgaagcca aacagctctt tgagcccctc catgctgaag tccgaaccgg cacttagtcc
     1081 ctcttccttc atcatgctgc ccaggtaggg ggcgatcatg tcgatctggt ccaggaagtc
     1141 agctttgagg cttgcttccg gggtgttgag gcggaaaaat tctctggcca gggcgtcgat
     1201 atcgttcgat tgcgccagga tgggaaacgc cagcaggcag atcacaagtg caagatatgc
     1261 tttcatcttc tctactcctt tgatgggata tgacagatgt ggccagggta gacaagtgcg
     1321 gtgggctgtc aatctttttc cttccgcggt ggatgctcat ggccccttgt ggaaactttg
     1381 caacgaaaac agccgatttt ggcatgaatc acgcttccag acaaaaaact aactcttgac
     1441 aagtctgtgg ctttcaatat catgtgaatc aggtatcgta taagagtaca aaccgaggaa
     1501 cgcggtgttg atgtaccaca gccagttgga atcgatgttt agcgtgcatc cctcgatgaa
     1561 atcttagatt tccattggtg gccccaggca tcgcgcccgg ggctttcttt ttgcccggat
     1621 gtggctaagt gcgcgggact ttggaggact tccagcccgg ctttcggcct tcgcgaacct
     1681 tagtctgatc gcccggggcg gcataaatca cttgactctt ttgagcttcc ccagtctgat
     1741 gcatacttac agagttttag ccaccgaggt atcaagatga agaaacgctt gctgatcgcc
     1801 accggcggag gtgattgccc tggcctcaat gccgtgatcc gtgccatagt gaaacgctcc
     1861 tcccatgagc acaactggga aatattaggc agcatcgacg ccttcaacgg cctcctgcta
     1921 gaccccatgc acctggaaga acttaccctc caacgcgtgg ccggaatcca cgtgaaaggc
     1981 ggcaccatca tcggtaccac caacaaaggc ggacctttcg agtggccggt caaaaacagc
     2041 gacggcacct ggagcaccgt ggaccgctcc aaagacttca tggaccgcct ccgctatcaa
     2101 aatatcgaag cggtgatcaa catcggcggc gacggttccc aacgcatttc ccaaaagctg
     2161 tttgagctgg gctgccccat tatcggagtg cccaaaacca tcgataacga cctctcggat
     2221 acggacttta ccttcggctt ccagactgcc gtcgatgcag ccacggacgc ggtggacaag
     2281 ctcgtgagca ccgcggccag tcatcacaga gtgttgatcc tggaagtgat gggacgctac
     2341 gcgggctgga ttgctctgca tgccgccatc gctggaggcg cggaagtctg tctgatccct
     2401 gaaatccctt atgacctcaa aaaagtgatc gaggtgatag accagcgggt ggacaacggc
     2461 aaaggctttg ccattatcgt gatcgccgaa ggggcaaaac ccatcgaagg cggcatgagc
     2521 tacagcgaaa acgacactcc cggcgccatg aaaatccgcc tcggaggtgc cggactgagg
     2581 ctttcccacg atctcaagga agcaggctcc tgtgtggaaa tccgcgaaac catcctgggc
     2641 catttgcaga gaggtggacg tcccaacagc tttgaccgca tcctggcctc catgtttggc
     2701 gtcaaggcct tcgagctcgt attggaagag aaatggggtc acatggtggc ctatcgccat
     2761 cccaatgtcg tagccgtccc gatcagcgac gccatcaagg aatacaacct ggtggacgtg
     2821 aattccgacc tggtgaagac ggccagggga ctggggattt cgctgggaga ctaggtgaga
     2881 tgccttcggc aggtgagata ctgcgtatgt gagagcttcg ctgtgagagc ttcgcgctgg
     2941 tgagggagtg gcgcctcctt aaaaccctaa aactctaaaa ccttaaaacc ctacaactcc
     3001 tctaccagct gcgcgtacag cgcttccagc ctgggcaggc gttcacggat gtcttccaat
     3061 gaagccacct gggtgagcca gattagctca tcggcggctt cggatgggta gtcccgttcg
     3121 gcgtagcggc tgccaaaatc ggctttccag ggcctgtatt tgaggttcat gaggctcacc
     3181 acatggcgtc ccaacagggc gttgtaattg cccagagcgc tcagccagtt ttctctggcc
     3241 agggcttttt tgagctcgat cagcatcagc caccaggtgc cggtagccat ggcatacatc
     3301 ctgtcccgca gttgtttaag ctcttcggga ggcatcggtt tggaactata gatagcgctt
     3361 ttgtcaaacc agatcaccgc gttgccgtga cgctccggtt cggtgagttt gttgggattt
     3421 tcctcaccgg tgacggcaat gtcgcaatag aagaggggcg gcagtttatc cagccggtag
     3481 aagcattggg aaagtccatg ccaggcgggc tcaggcattc taaagctctc caggacgccc
     3541 cactggcttt caaacagctc gttcatggcc tcaaacacag gctccgcgga gcttccctgt
     3601 ttggtgacca ccagcaaatc cagatcggaa tgctcgtcca gatatcccgt ggcggcggaa
     3661 ccgccttccc aggctgccac tacgtcgtct ctgctctcca acagagcctg cagcctggcc
     3721 ttaagtgtca ggacgcgatc gtccatcggc taactccttt atccattata tccatagcgt
     3781 tccagatcta tctttccgga agcgtcgaag accactcctt cggcctccag catagagcgc
     3841 tgcaaagctc cgccgcattc ttctgacagg ccaatgctcc catccgatct cacaactctc
     3901 caccagggaa gatcttggga atcgctctga gtgtgcagga ttctcaccac caatctggcc
     3961 ccgtgcgcta ttccagccgc ttcagccacc ctggcgtaac ccatcaccct tccgggaggg
     4021 atggccccga tgtggtctaa aatggcctga gtggagctat tcaccaaggc ctcagcggat
     4081 gatccccgtc accgtttcac cgggattgac gacaccgcca tgggctttga cgcggtggta
     4141 gaagatggtg tagctcatat ccacataggg ggccagccag aaaatcccga tcccaaaagt
     4201 gaacaggctc aggaaaaacc agcctataaa ggagaggctc agcataaaaa gctcggtctt
     4261 gtggcccagc atcatctctt tgctgagggc catcgcctgg ctggcgctca gctggggatt
     4321 ttccaccaga ataaaaaagg tcatgctgta tcccagcgcg gcaatgatgc caggaacgat
     4381 cagcaagaag ctccagagga tcacatagac gctgatcagc aatccggaga tcaccgcccg
     4441 ggaaaaatcc ctgaacccgt tgaagacttc ctctatctgg aagttgtgcc cttcataaag
     4501 tttgagaaat atggcagtta cgcccagatt gagaggagcg cccaccagaa tactcagggg
     4561 tgagctcaaa aactgcgtgt tgcccagccc aaagatcagg ctgacgctac cgctgagcac
     4621 actcacgata aacatcagca gcgcgcaggt attccaccgg cctctcagaa ggtcgcgggc
     4681 ttcggccctt agttgtgtgt agtccaaatc tatacctctg tttcagcata ctttgcaaag
     4741 catcgtttcc tcataagctc ctgctgtcaa gctgataatc gatcttgagc cggaggcctt
     4801 gtggaaataa ataaatcgct tgacaggatg gggcaatcaa tagccttgtg tcaaaggtgg
     4861 aattatgaag cattactgtt tgatggtcat tagcttcctg tgtctgttca ccgctttggg
     4921 agcagcttcg ccacagtatc gcgtcctcct ggagtgggat gagatggagg gcgagctcaa
     4981 cggcatcctg agcgggatcg tcgacagccg ttacggattt atccagggtg ccggacccgg
     5041 tcaggtcctg gacggggcca tcagttccag aggcgaaaac actatgcggg gcgcttctca
     5101 ggaattctat atcaacatcg cggacggcta ttacagtttt tggatcaggg acaaatttgc
     5161 cgatgacgac atcaatcccg atcccaatct gatcagggcc gccaagccac gcgtcagtgt
     5221 ctatcgcggt aacgctctgg taggcagggt ggacatcacc cagggcgcgg gcctcacctg
     5281 caaggtcttt actctggatg cctccaatgg cgcttttgat caggaaatcc gcttctttcc
     5341 ccgcgcccgc atctttgccg tgatgatcgt cgatgccatc aacggcaatg gcctacccaa
     5401 tgtgcagatc agcatcaccg gaggggaaga ccgctatcca tccgtgacca ccgatgccag
     5461 cggctttgcc tacatccccg tcgagattgg cagctatcgt ctggaaatgt ataaagaggg
     5521 ctacatcaac accagttttc tggtcagtat gggctttgac gaaaaccccc gcgagttcgt
     5581 cttcgccatc gctcccaagg ccaaggacta tcgcatcgtg ctcacctggg gctcccgtcc
     5641 caacgacctc gatgcccatc tgaccggccc caaaccggaa gggggagact tccacatctg
     5701 gtatcgcaac cgtatcctga tcgccggaag ggactttctg gaccgcgacg acacggataa
     5761 atacggcccc gaaaccgtca ccatctacaa acccgcgtcc ggtgaataca gcttttgcgt
     5821 gcacgactat tccaaccgtt ccgaccgctc ctcccggatg ctttctttca gcaatgccgt
     5881 ggtgcaagtc tatgccgatg acgctctcaa agcaaccttt acagtcccgg agggccagtt
     5941 tggcaacctc tggcgagttt ttaagattga caaaaatcaa cgtgtcgttt ctgtgaatac
     6001 cgtcggctgg accgacgacg agaaaaacat cagataaccc ataaatggag gaaccgatga
     6061 agtccaaaac aatgttattc gttatactgg ccgtagttct gctggtagcc ctgaccgcct
     6121 gtagcaagga caagaagacc cccgcccccc aagccgaagg tgaagtggta aacttccccg
     6181 cctggtggaa cactcaaaac gacgacgcct atgtctgcac ctatggcatc ggcaccaacg
     6241 tcaaaatgac cacatccatg gacaccgcca aaaacaacgc cctgtttgag gccgcccaat
     6301 acgtggaagt ggaagtgaaa ggcatgctca agacctacga agaagaagcc ggcgtctttg
     6361 atccccagct tctggccctc acctccaaag tcatcaaaac cgtggccgac gccaccttct
     6421 ccggcgtcat caacggccag accgagaccc gcaaagtcac cgaatttggc ggtccccgct
     6481 ataccaccta tctgcagctc aagatcccca aaaccgaagt gaaaaagacc ctctacaaca
     6541 acatccgcaa cgaagaggcc ctctacaacc agttcaaagc ctccatggcc tttgaggaac
     6601 tcgagcgcac cgtgggccag taaactgtcc ctttttcaaa tccttgagcc cggttatata
     6661 ccgggctttt tttagggccg aatacaaata agcccatctc cctagcccgc ttgcatgtag
     6721 cagcaccgag atgaagagct gggagtcctt ttttccccct tccttcctga atagcattac
     6781 ggtatcatta cggactcatt acgggtgaag tccgtaatga gtccgtaatg ggatgcgggg
     6841 aggaataggg aagagggcag gattttttag ctcgtgggga aaccggcagg ataacagcag
     6901 aaaaacagca gggaccacaa caggcgaaat atttctgaga gcgttgaata atctgtatca
     6961 ctggtttcag agcgtgtgcg aaaacatgcc gtcttggagt tcaaagcccc taaatagatt
     7021 atccttgtat ctttatacct gacggggctt tggacttcaa gcagcgcatc gaagacattt
     7081 atctcgcggg tgtactggtt ggagttcaaa gcccctggac agaataggcg tttatctcct
     7141 tgcttgaggg ggctttggac ttacgtgcag cacatcgaag acatttatct cgcgggtgta
     7201 ctg
//
LOCUS       DAOSFN010000130         7099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_9210_length_7099_cov_10.078507, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000130 DAOSFN010000000
VERSION     DAOSFN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9210_length_7099_cov_10.078507"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..3708
                     /locus_tag="PLO35_08085"
     CDS             322..3708
                     /locus_tag="PLO35_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOH79669.1"
                     /translation="MKSFIIVVLVLIAVCPAFAEWTIVASYPISGKASGLASDGTYLY
                     YGIYGTDGGRVFRFDPVSGQETLLFNNTTINDSYGMSYNSQHLWITDHTTSPSAPAFA
                     LQLDLTGNIIGQFNLPDHYMSGIAYDGGNFWVMTYYPDPGTVYKVTPTGTILQQFTPP
                     NNQPWDICKQGDNLWIADYNANMISKVSQTGTLLESQTSEIQKPAGIAWDGQYLWYVA
                     GALSAPSTLYKVDLSGTGTPEIGVSFDAHDFGNVVMNQTSTVDLTISNTGTGDLGLSS
                     ITFSSVSYSTSATLPITLAPNGSTVVPITFSPDNWGEFPAEMSIHSNDPLSPVQLVNL
                     SGYGVFPSAHIEVSPSALDYGPARIDADTGRFFTLSNQGNTSLQINNISFSNPAFFVD
                     GSIQLPITLAAREEYPLRIWFSPGSVGEINAMASLGSNDTGNPQQSINLTGAGMTVDL
                     AMGAQLWDFQTLPGTFTNIRAIKAISDIWGNGKDDLMICSEDYYLRALNGNSSGTADV
                     IWEQYIYSGPVSYYRGLYVTGDLNNDGIQDVVIGTAGGDRSVRAYSGKTGTPLWMFST
                     TMYGEGGAVYQVDARRDFNNDGIPDVLAATGDDIYEAGPKRIFLLNGVTGAMIWERYA
                     FGPAFSVISVADFTADGIPDVIAGASNEAETQAWVRAINGATGAIAWSAQLAGTSVWA
                     LAQIDDLNGDGVPDLMAGSFQGSGHYYAYNAATGGLIWSGSTGASLILQLEVLGDVNN
                     DGYNDIAIGHVTPHTAVISGLTGQYIWSQSTADNAWYLANGGDLTGDGINDIFVGTLY
                     QNNAAYYVNGTDGTILSTLWTGTPVDAIGALPDVTGDNSREMIIGGRNGSVRCFSGGP
                     VTLPNPGFISGNVSISGGPGLVTNVVVTAGTVSASPNAEGDYTLTTAPGTYEVTASLP
                     GYYAAPVSNVTVTAGSTTPDIDFTLQLLPLLPPVDLTINAQNGVINWLPPSSTPQYLP
                     SSYKIFLDGTLVGNTEELHWTLSGLTPNTSYLAGVSCLYPTGESEAATLQFTFTGSGA
                     DDPTLPHMTRLWGNYPNPFNPETVIHFSLASRSPVIIDIFNVKGQRVKRLVQADLDRG
                     DYHRTWDGRDESHAQQASGLYFYRFTAGSHTETGKMLMMK"
     gene            complement(3781..4335)
                     /locus_tag="PLO35_08090"
     CDS             complement(3781..4335)
                     /locus_tag="PLO35_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH79670.1"
                     /translation="MMELITSRHSVRDFLPDPVPKEVLDEMLEAARLAPSAQNKQPWR
                     YLVFTDPERIKELARHCGLIGLSNFFIKQAPCLVIACAETKKNTRINAQDYYLVDVAI
                     SMQQMMMAALAHGVGSCWLAAFSEKKLREYLKLPKSWKVVAMSPFGYPKEDKSLYTGM
                     LSSFAGSAKRLPLEEIAWVDTPMN"
     gene            complement(4341..4850)
                     /locus_tag="PLO35_08095"
     CDS             complement(4341..4850)
                     /locus_tag="PLO35_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOH79671.1"
                     /translation="MPLSELLAIIPASGLGSRFGMPKADAEIDGTSFAEHITGLLKAG
                     GVANIVLARYPDSPDMLSSIKRAVLEHPGFRAWLIWPVDHIFVRPATLQALLDTWTAM
                     PDALIRPVLAGRNGHPVIVPAWLDLEADHHDQGLAGLIREQVCTVIDVPVEDMGILRN
                     VNRREDMEI"
     gene            complement(4837..6285)
                     /locus_tag="PLO35_08100"
     CDS             complement(4837..6285)
                     /locus_tag="PLO35_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOH79672.1"
                     /translation="MPKLITGRDEAVIKKNALRCRQRGIIMPTIRQQMFPETIPDSIK
                     QRLKPIGLWDIDPLNLFRITWKNDVNTGLFGAPNYFEVPRELTGVKARVIGLVGKYFP
                     TGAHKVGAAYGCLAPRLVTGSFDPEIHKAVWPSTGNYCRGGAFDCALLACPSVAILPE
                     EMSQERFSWLKEIGAEVYATPGCESNVKEIYDKCDELRPDPQYIILNQFDEFGNPFWH
                     YHVTGDAIHEVWNLIKTDKSRLSGFVSATGSAGTIAAGDYLKKHFPLMKTTASEALEC
                     PTLLNNGFGGHRIEGIGDKHVPWVHNVRHTDAVAAIRDEDCMRLLRLFNEPAGQEYLI
                     SEGISAEVVQNLPLLGISSIGNLLASIKQAKYFELNEDDVIFTIFTDSAGLYESRVQE
                     QRALKGEYNAMQAALDREAALNCQQYDNYLELSYQDRKRIHNLKYYTWIEQQGKTYEE
                     ILAQWEPEYWQETFETNLDELDASIEAFNALI"
     gene            complement(6544..6966)
                     /locus_tag="PLO35_08105"
     CDS             complement(6544..6966)
                     /locus_tag="PLO35_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOH79673.1"
                     /translation="MTESELNELSGNLIGACIEVHKYLGPGLMESAYQECLCHELSLR
                     GISYVKEYDISIEYKGVVIPTRLRADMLIESSIIMELKSVSELIPVHEAQLLTYLKLT
                     GCQLGLLINFNVPLLKDGLKRVKYYPKPSHVPYPNHGQ"
BASE COUNT         1608 a         1937 c         1876 g         1678 t
ORIGIN      
        1 tcattgttac gcgtggcgat aaagactttg cagtcttcac attcatgtcc gcagggggct
       61 aacatcggct tgctccttcc ttaaccgtgg cgttatttac tatctgcatg cctggcaaca
      121 tcatccagca tgagatacgc atgggatgcc aatcttcctg aagcggtttc ctgtcaatcc
      181 caaatcagcc tcccaggctg gaattatggg cctgccgcag aataacagaa aaaagacatt
      241 gacaggaatt atggactgtg atctattgct gtttgcttgg atagattgta acattataaa
      301 taatagccca tggaggttat catgaagagc ttcattattg tggttctggt attgattgct
      361 gtctgtccgg cctttgccga atggacgatc gtggcatcct atcccatcag cggcaaagct
      421 tccggtctgg catccgatgg cacttatctt tattatggca tctatggcac agacgggggc
      481 cgggtattcc gtttcgatcc agtcagcggc caggaaacgc tgcttttcaa taacaccacc
      541 ataaacgaca gctatggaat gagctataat agccagcatc tctggatcac agaccacacc
      601 acatctccat ccgcgccagc ttttgccctg cagctcgatc tcactgggaa catcattggc
      661 caattcaacc tgcccgatca ttatatgtcc ggcattgcct atgacggtgg caatttctgg
      721 gtaatgacct actatcccga tccgggaacg gtatataagg tgactcctac cgggaccatc
      781 ctgcagcaat tcactcctcc caacaaccag ccctgggata tctgcaaaca gggcgacaac
      841 ctctggatcg cggattacaa cgccaatatg atcagcaagg tttcccagac gggaacgctg
      901 ctggaaagcc agacttccga gatccaaaag cctgccggta ttgcctggga cggccaatac
      961 ctctggtatg tagccggagc cttgagtgct cccagcaccc tctataaggt ggacctgagc
     1021 gggaccggaa cccctgagat cggcgtctct tttgacgcgc acgactttgg aaatgtggtc
     1081 atgaaccaga cttccacggt ggacctcact atctccaata ccggaacggg cgatctgggc
     1141 ctgtccagta tcaccttttc ctcagtttcc tattctacaa gcgccaccct gccaataacc
     1201 ctggcaccaa atggcagcac ggtggtgcca atcacattct ctccggacaa ctggggcgag
     1261 tttccagctg aaatgagcat ccattccaac gacccgctct ctcctgttca gttagtcaat
     1321 ctctccggat atggagtgtt tcccagcgct catatagaag tgtctccctc cgcgctggat
     1381 tatggccccg ccaggataga tgccgatact ggcaggtttt tcacgctcag caaccaggga
     1441 aacaccagtc tgcaaatcaa caacatcagc ttctccaatc ccgccttctt tgtcgatggc
     1501 tcaatccaac tgcctattac tcttgccgca agggaggagt atccgctgcg gatctggttc
     1561 tctcctggct cggtcggaga gattaatgcc atggccagcc tgggaagcaa tgacaccggc
     1621 aatcctcagc aatctatcaa tctcacgggc gcggggatga cggtggacct ggcgatgggt
     1681 gctcagctct gggacttcca gaccctgccg ggcaccttta ccaacatccg ggccatcaaa
     1741 gccatttccg atatctgggg caacggaaag gacgacctga tgatctgcag cgaagattat
     1801 tatctgaggg ctttgaacgg caactccagc ggaaccgcag acgttatatg ggagcaatac
     1861 atctattccg gacccgtgag ctattaccgg ggcctgtatg ttaccggcga cctcaacaac
     1921 gacggcatcc aggacgtagt cattggtacg gcgggaggag acagatcggt gagggcctat
     1981 tccggtaaaa ccggaacacc gctctggatg ttcagcacca ccatgtatgg agagggcgga
     2041 gctgtctatc aggtggatgc caggcgggat ttcaacaatg acgggattcc ggacgtgttg
     2101 gcagccacag gtgatgatat ctatgaggcg gggcccaagc ggatattcct gctcaatggg
     2161 gtcaccggtg ccatgatctg ggagagatat gcctttgggc cggccttttc cgtgattagc
     2221 gtggcggatt ttacggctga cggcatcccg gatgtaattg caggagcttc caacgaagct
     2281 gagacccaag cctgggtgcg ggccatcaat ggagccacgg gagcgatagc atggtcagct
     2341 caactggcgg gcacttccgt ctgggctttg gcccagatcg acgacctcaa cggcgacggc
     2401 gtcccggacc ttatggctgg ttccttccag ggcagcggtc attactatgc ctacaacgcc
     2461 gccactggag gactcatctg gtccggctcg acaggtgctt ccctgatcct gcagctggaa
     2521 gtgctgggag atgtcaacaa tgacggctac aacgacatcg ccattggcca tgtcactccc
     2581 cacacagcag ttatcagtgg actcaccggc caatacatct ggagccagag cactgccgac
     2641 aatgcctggt acctggccaa cggtggagac ctgaccggcg acggcatcaa cgatatcttt
     2701 gtgggcaccc tctatcaaaa caacgccgcc tactatgtca acggcacgga cgggaccatc
     2761 ctctccaccc tatggaccgg caccccagtg gatgcgatcg gagctttacc tgatgtgact
     2821 ggagacaatt cccgggagat gatcatcggt ggcagaaacg gctcggtccg ttgcttctcc
     2881 ggaggtcccg taactctgcc caatccagga tttatctctg gcaatgtctc tatctcagga
     2941 ggtcccggtc tcgtcaccaa tgtggtcgtg acggcaggaa ccgtgagcgc cagtcccaat
     3001 gccgagggag attacactct gactactgcg cccggcacct atgaagtaac ggcatcactg
     3061 cctggatatt acgccgcgcc agtctcaaat gtgaccgtca cggcgggcag caccactccg
     3121 gacatagatt tcactttgca actgctgcct cttttgccac ccgtagatct taccatcaat
     3181 gcccagaacg gtgtaatcaa ctggttgcct cccagttcga caccgcagta tttaccaagc
     3241 agctacaaga ttttcctgga tgggactttg gtgggaaaca ctgaggagtt gcactggaca
     3301 ctcagtggcc tgacccccaa tacctcctac ctcgctggag tgagttgtct ctatcccacc
     3361 ggagaatcgg aagcggccac tctgcagttc actttcaccg gctcgggagc cgacgatccc
     3421 acgctgcccc atatgaccag gttgtggggc aactatccca acccctttaa tcctgaaacc
     3481 gttatccact tttctctggc atcgcgatcc cctgtgatta tagacatttt caacgtcaaa
     3541 ggccagaggg tgaagagatt agtccaggcc gatctggacc ggggtgatta tcaccgaacc
     3601 tgggacggca gagacgaatc tcacgcccag caggccagcg gactgtattt ctacagattt
     3661 acggccggca gccataccga aacaggcaag atgctgatga tgaaatagca aagtgggagt
     3721 ttcacgctta aactctgttt gaccacccct tgggtttcaa ccagcgtcca ggggtgaaga
     3781 tcaattcatg ggtgtgtcca cccaggcgat ctcctccaga ggcaagcgtt tggcagatcc
     3841 ggcaaagctg ctcagcatgc cggtatagag gcttttgtcc tctttgggat agccaaaggg
     3901 agacatcgcc accactttcc agcttttggg caacttcaga tattcccgca gtttcttttc
     3961 gctaaaagcg gccagccagc atgaacccac cccatgggcc agtgcagcca tcatcatctg
     4021 ctgcatagag atcgccacat ccaccagata atagtcctga gcgttgatgc gggtgttctt
     4081 tttggtctcg gcgcaggcga tcacgaggca gggggcttgt ttgataaaga agttggatag
     4141 cccgatcagg ccacagtgtc tggccagttc tttgatgcgc tccggatctg tgaaaaccag
     4201 atagcgccag ggctgcttgt tttgggcgga aggggccagg cgggccgctt ccagcatctc
     4261 gtccagcact tctttgggca ccggatcggg cagaaaatcc ctcacgctgt gccggctggt
     4321 gatcagttcc atcatatcca tcaaatctcc atgtcttccc tacggttcac gtttctgagg
     4381 attcccatat cttccactgg gacatcgatc acggtgcaga cctgttcgcg gatcaatccc
     4441 gccaggccct gatcatgatg atcggcctcg aggtccagcc aggcaggaac gatcaccgga
     4501 tggccgttgc ggccagcgag aacaggtctg atcagggcat ccggcatggc ggtccaggta
     4561 tccagaagcg cctgcagggt ggccggtctc acaaagatgt ggtccaccgg ccagatcagc
     4621 caggccctga agccaggatg ctccagcacc gctcttttga tgctggacag catatccggg
     4681 ctgtcgggat atcgggccag cacgatgttg gccactccac cggccttcag gaggccggtg
     4741 atatgctccg cgaagctggt cccatcgatc tccgcgtccg ccttgggcat accaaaacgg
     4801 cttcccaaac cggacgcggg aatgatggcc agaagctcag ataagggcat tgaaagcctc
     4861 gatggaggca tcgagttcgt cgaggttggt ttcgaaggtt tcctgccagt attccggttc
     4921 ccactgggcc aggatctctt cataggtctt gccctgctgc tctatccagg tgtaatactt
     4981 gaggttatgg atgcgcttgc ggtcctgata actgagctcc agatagttgt catactgctg
     5041 gcagttgagg gccgcttcac ggtccagagc ggcctgcatg gcattgtatt cacccttgag
     5101 ggcacgctgt tcctgcactc tggattcata aagcccggca gagtctgtaa agatcgtgaa
     5161 gatcacgtcg tcttcgttga gctcaaaata cttggcctgc ttgatggagg ccagcaggtt
     5221 gccgatcgat gagatgccaa gcaacggcag gttttgcacc acttcggctg agatcccctc
     5281 agagatcaga tactcctgcc cggcaggttc gttgaagagc ctcagaaggc gcatgcagtc
     5341 ttcgtcacga atggcggcaa ccgcatcggt gtggcgaacg ttgtgcaccc agggcacgtg
     5401 tttgtcaccg atgccttcga tgcggtgacc gccaaagccg ttgttgagca aggtggggca
     5461 ttccagggcc tcggaagcgg tggtcttcat aagggggaaa tgcttcttga gataatctcc
     5521 agcggcgatg gttccagccg aaccggtggc gctcacgaaa ccactcagac ggcttttatc
     5581 cgtcttgatc agattccaca cctcatggat agcgtcgccg gtcacatgat agtgccagaa
     5641 ggggttgccg aattcgtcga actggttgag gatgatatac tggggatcgg gacgcagctc
     5701 gtcgcacttg tcgtagattt ctttgacgtt ggattcgcag ccgggagtgg catagacctc
     5761 ggcgccgatc tctttgagcc agctaaagcg ttcctggctc atctcttccg gcaggatcgc
     5821 cacgctcgga caggccagca gggcgcagtc gaaagctcca ccgcggcaat agtttccggt
     5881 agagggccag acggctttgt ggatttcggg atcaaagctg cccgtcacca atctgggggc
     5941 cagacagcca taagcagcgc caactttgtg ggccccggtg gggaagtatt tgcccaccag
     6001 accgataacg cgggctttca caccggtgag ctctctgggc acctcaaagt agttgggcgc
     6061 gccaaagagg ccggtattga cgtcattctt ccaggtgatg cggaagaggt tgagggggtc
     6121 gatatcccag agcccgatgg gcttgagacg ctgtttgatg ctgtccggaa tcgtctccgg
     6181 aaacatctgc tgacgaatgg tgggcatgat gatgccccgc tgcctgcaac gcagagcgtt
     6241 ctttttgatg accgcttcat cgcggccggt gatcagtttt ggcatgtatt tactcctaag
     6301 attttactac tgaggtacag aggattcttt tttcactaca gagctgcaga ggaaacagag
     6361 agagttacag aggaatctaa tgggtagtaa tctgtttcat aatttccttc agttctttcc
     6421 tgggaaattt ctgtaaaccc atactgacaa agtgattttc tgctattgtg aacaatgaga
     6481 tgcagaggat tatttttcac tacagaggta ctgaggcaac agagagagtt acagagggta
     6541 gatttactga ccatgattgg ggtaaggaac gtggcttggc ttgggatagt atttgaccct
     6601 cttcagacca tcctttagta ggggaacatt aaagttgatc aatagaccaa gctggcaacc
     6661 cgttagtttc agatatgtga gaagctgggc ttcatggact ggaataagtt ccgatactga
     6721 tttcaactcc atgatgatcg agctttcaat cagcatgtca gcacgtaatc tagtgggaat
     6781 cacaactcct ttgtattcta tcgatatgtc atattctttg acgtagctaa ttcctcgcag
     6841 gctcaactcg tggcataggc actcctgata agcggattcc atcaatccag gaccaagata
     6901 cttatgtacc tcaatgcagg caccgatcag attgccgctc agttcgttaa gttctgactc
     6961 cgtcattctt cctcctaagg gattaatctc tattgttatt atcagtttct aaacgcttta
     7021 tctgtatata cctattctgt caaggccgca ataggccaat caagtatatc catcataatt
     7081 tacttctctg tatctctct
//
LOCUS       DAOSFN010000131         6959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_9454_length_6959_cov_8.923233, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000131 DAOSFN010000000
VERSION     DAOSFN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9454_length_6959_cov_8.923233"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    19..118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(357..4253)
                     /locus_tag="PLO35_08110"
     CDS             complement(357..4253)
                     /locus_tag="PLO35_08110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HOH79674.1"
                     /translation="MTKLSVTLLIICISLSVLNAGLTELTDTAGNPIEAGTIQFKLRP
                     EYKSALSSGAVLTGISELDARLQGLGVNSVKPRFRYDHAQKPAGAPDLSLILEARFSP
                     SVHPLAVINSLSLDPRVEYAEPVYIDAVLAVPNDPNYPSALYFVPLMAEAAWDVHKGE
                     DGAQPVILAVMDTGVRWSHADLGANLWNNLGEDANSNGYTTYYNGSAWIYDAGDLNGL
                     DDDGNGKADDLIGWDFMLNANGDQHYDPYESGGHGTSVSGIANARTNNSTGIASLAWN
                     VQLMPLSGSHPGAPSSVYRGYDGILYAAEMGADVINCSWGGTGFSQAAQDVVDYASAL
                     GTIVVAAAGNSGNTIPIYPAAYANVIAVASVNNAGVKYSGSNYGGYIDVAAPTESVGA
                     FAGSGYTLVSSATSYASPIASSLTALIRSYNPSWTRAEVIDRLLGSCDNIDALNPGKE
                     NALGQGRLNAYRALTDVNPAPDDELRFSLLRQLPPTETNGNLAVEPDETFSLNLMLRN
                     HSLAGSGAVNYVLSSSDPLVNILQPNHSGTAGPDSFVELNDAFEVYVSPSATSKYVSF
                     TLAISADKPIVQGASLSFTVLIQAGGHFVWEGVASGRDMSGAYIRNRLQAMSLPVVYG
                     TGFPASLYGFDSVWLSFGMARGNIVRFDSPSMYNALKDYLESGGRVYIEGGDVVGFDM
                     GNYLPDVEGSLDAHEVLWPLLGLDSASDGTLNPIDGLSGEEGWICRPLWFSASNQTVS
                     YDIDTYVPGPNAYPAFIESAYGIVGHEYVGSYGQRVFLSAYPIAELVDGTSPNTRAEL
                     VSRVAAFFTSPAPAMPYIAAIPSGDELLLPIFMQGESVSGINDNRLPMASRIRLTGLN
                     PGSTYRYTHQMVKTSDTPLASGAGTPIFVDEAGLFNRVESPSLSAPGSYGSFISDAGG
                     EYTGWFVIEATGDQRFDSGTPLHLRILLNDGAGGTTPQSFLTSSSTLQTLGFGTQSLS
                     SLGTAVAGTSNAGSHNFVFAYDDASGSGRPIAGNFIEDSELDFSAAHYPGFYQNHVSG
                     VEGAYGLIIPNHLPGKAFGGIKRLEARAGSDGSLIAVHSDDDGIWPGGANTVDPSGGE
                     ASPIFFTPQDATLPVTLSSFTAVQSGSQALISWTTQTETAMSGYLVWRAETLSLPGAV
                     SISPLILAQNSPNPVRYEFRDLEAGMGTWSYYWLEARSLDGNSLFHGPIALLITPPGA
                     EESPEIPLRSGFDPIWPNPFNPSANLSWRLRKSGDVSLEVYNLRGQLISRQSWPIQAA
                     GYHRLSLDGSGLPSGVYLFRLDTGDGVFVRKAVLAK"
     gene            4427..4753
                     /locus_tag="PLO35_08115"
     CDS             4427..4753
                     /locus_tag="PLO35_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HOH79675.1"
                     /translation="MIITTTPNIDGYKVDHYHGIVTGEAIMGANIVRDLMASITDIVG
                     GRSKAYESKLEDGRNEAFREMTAKAERLNANAILGVDIDYEVIGQSMLMVAVSGTAVT
                     VSLKES"
     gene            complement(4806..5972)
                     /locus_tag="PLO35_08120"
     CDS             complement(4806..5972)
                     /locus_tag="PLO35_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79676.1"
                     /translation="MRTILVLPLCFLLVLICLPWQLSGAESIARNLSPVVSNVSASQR
                     LDGSKIVDITYDLNDPEGDSCAVSLRLSSNGGTSFDIVASAVRMSGDVGADIAPGPGK
                     HIVWDAGGEDFELDGGYFYRVYADDGFTTPPPPGFILVEGGSFYNGYSTVTLGSYYLA
                     QYELTQLAYQQVMGSSPSWGYGVGDSYPVYQVSWFKALEYCNRRSLQEGLTPCYQFSV
                     YGSDPDNWPAGWDLDYGNHQYVSCDFNASGYRLPTDMEWEFAARGGNLSNDYIYSGSN
                     IPGEVGWYYDNSGSYSQGHPDYGNHPVGLKLPNELGFYDMTGNVWEWCWDIYGALPNT
                     PQTNPTGPLDGDHRIIRGGSWVNFAGIVEVDFRWYYFASHIDINIGFRVLRKVL"
     gene            complement(5969..6745)
                     /locus_tag="PLO35_08125"
     CDS             complement(5969..6745)
                     /locus_tag="PLO35_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79677.1"
                     /translation="MIKVYLCLLCGAFMLSLGAGTGHGTSALVINDTVSPELGIVAPN
                     GGENWFIGETYQIQWNASDSNLIPNSVYIWYSLNGGTGYIPIAEGTANDGTHSWTAPA
                     YQTEAARVRIRVADYFGNFTRESSQQSFSISYVPPAEPQNLRVDISNDEDAVITWDAV
                     SHTILPYNYPIVPDGYIVLYNETPSTDDPYFYFLAETDQLEHTHPRVVRFRDSMFYKV
                     IAYKDYDQRMATLLASLKPGDRISLAELRTCLRGWKGGLK"
BASE COUNT         1493 a         1895 c         1983 g         1488 t
ORIGIN      
        1 cgttatgcgt tacgcattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
      121 catgcacaca tcagttccca tcctctgtct ctcaagagtc tctgtccctc cgttcctctg
      181 ttttagaaaa acctctgctt ttctctgtgt tctaaatcgt tatgccttat gcgttacgca
      241 ttcgcatgca cacatcagtt cccatcctct gtctctcaag agtctctgtc cctccgtccc
      301 tctgttttag aaaaacctca gcttttctct gttaatcctc tgcttttctc agtgttttac
      361 ttcgccagca ccgctttgcg gacaaacact ccatccccgg tgtcgagcct gaatagatag
      421 actccgctgg gcaaaccaga gccgtccagg ctcaaccgat gatagccagc ggcctgaatt
      481 ggccagctct ggcggctgat cagctggcct cgtaggttat agacttccag gctcacgtca
      541 ccgctcttcc ttagtctcca gctcaggttg gcgctgggat tgaagggatt gggccaaatg
      601 gggtcaaagc cgctccgcag agggatttcc gggctctcct ctgctccggg aggagtgatc
      661 aggagcgcga tgggaccatg gaagaggctg tttccatcca gggagcgcgc ttccagccag
      721 tagtaactcc aggtccccat gccggcctca agatcacgga actcatagcg taccggattc
      781 gggctgttct gggccaggat caaagggctg atggatacag cgccaggcag gctcagcgtt
      841 tcggcccgcc agaccagata gccggacatg gccgtttccg tctgggtcgt ccagctgatc
      901 agggcctggg agccagactg aacggcggtg aaggaagaca gcgtcaccgg cagggtggca
      961 tcctggggag tgaaaaagat aggactggcc tcgcctccgg agggatcgac ggtattggcc
     1021 ccgcctggcc agatgccatc gtcatcacta tggacggcaa tcaggctgcc atcgctgccg
     1081 gctctggcct cgaggcgttt tattccgcca aaagccttgc cgggcagatg gttggggatg
     1141 atcaatccat aggctccctc caccccgctg acgtgattct gataaaagcc gggataatgg
     1201 gccgcactga agtccagctc gctgtcctct ataaagttac ccgcgatggg acgtccactg
     1261 ccggaagcgt cgtcgtaggc aaacacaaag ttgtgactgc cggcattgga ggttcccgcc
     1321 accgcggttc ccaggctgct aagtgactgg gtgccaaagc ccagagtctg gagcgtggaa
     1381 ctactagtca gaaagctctg gggagtagtt cctcccgctc cgtcgttgag cagaatcctc
     1441 aggtgaagcg gcgttccgga gtcgaagcgt tgatctccgg tagcttcgat gacaaaccag
     1501 ccggtatatt cgcctcccgc gtcgctgatg aagctgccat aagaaccagg ggctgacaga
     1561 gaggggctct ccacccggtt gaagaggccg gcttcatcca caaagatggg ggttccggca
     1621 ccagaggcaa gaggagtatc ggaggtctta accatctgat gcgtatagcg gtaagtgctg
     1681 ccggggttca agccggtcag tctgatccgg ctagccatgg ggagacgatt gtcattgatc
     1741 ccagagacgc tttcgccttg cataaagatg ggcagcagca gttcatctcc gcttgggata
     1801 gcagcgatgt aaggcatagc cggtgcggga cttgtgaaga atgccgccac ccggcttaca
     1861 agttcggcac gggtgtttgg cgaggtcccg tccaccagtt ccgcgatcgg ataggcgctc
     1921 aggaagacgc gctggccata gctgccaacg tattcgtggc ccacgatccc gtaagcgctt
     1981 tcgatgaaag ccggataggc attggggcca ggcacatagg tgtctatgtc atagctcacc
     2041 gtctggttgg aagcgctgaa ccagagagga cggcaaatcc agccctcttc accgctgagt
     2101 ccgtcgatgg ggttgagggt gccgtctgaa gcggaatcca gtcccagcag cggccacagc
     2161 acttcatggg catccaggct gccttccacg tccggaaggt aattgcccat gtcaaaaccc
     2221 accacgtcgc cgccttcgat atagaccctg ccgcctgatt ccagatagtc cttgagggca
     2281 ttgtacatgg aggggctatc gaagcggaca atgttgcctc tggccatgcc aaagctgagc
     2341 cagacgctgt caaagccata caacgaggcg ggaaaaccgg tgccatagac cacgggcaag
     2401 ctcatggcct gcagacggtt gcggatatag gcgccgctca tgtcgcgtcc cgaggccacc
     2461 ccttcccaca caaagtgtcc accggcttga atgagcaccg tgaaactgag gctggcgccc
     2521 tgaacgatgg gtttatcggc actgatggca agcgtgaagc tcacgtattt gctggtggcg
     2581 ctgggggata cgtatacttc aaaggcgtcg ttaagctcca caaagctgtc cggacctgcc
     2641 gtgccgctgt gattgggctg gaggatgttg accagcggat cggagctgga gaggacatag
     2701 tttactgccc ctgagcccgc cagcgagtgg ttgcgcagca tgaggttgag gctgaaggtt
     2761 tcatccggct ccacggccag gttgccattg gtctcggtgg gaggcagttg tctgagcagg
     2821 ctgaagcgca gctcgtcatc aggcgcggga ttgacgtctg tgagggcgcg gtaggcattc
     2881 agtcttccct gtccgagagc gttttccttg ccgggattga gggcatcgat gttgtcgcag
     2941 ctgcccagca gacggtcaat cacttccgcg cgggtccagg agggattgta ggaacggatc
     3001 aaagccgtga ggcttgaagc aatggggctg gcataagagg tggcgctgct caccagggta
     3061 tagcccgaac cggcgaaggc ccccacgctt tctgtgggcg ccgccacgtc aatatagcct
     3121 ccataattgg agcctgagta tttcactccc gcattgttaa ctgacgccac ggctatcaca
     3181 ttggcatagg cggccgggta gatcgggatg gtgtttccac tgttgccagc ggcagctacc
     3241 acgatggtcc ctaaagcgct ggcgtaatcc accacgtcct gagcggcctg tgaaaaacct
     3301 gttcctcccc aggagcagtt gatcacgtca gctcccattt ccgccgcgta gaggatgccg
     3361 tcatagcctc tgtacacgct ggatggagct cccgggtgcg atcccgagag gggcatcagt
     3421 tgcacgttcc aggccaggga cgcgatgccg gtggagttgt tggtgcgcgc attagcgatc
     3481 ccggagacgc tggtgccgtg tcctccgctc tcatagggat cgtagtgctg gtcgccattg
     3541 gcgttgagca taaaatccca gccgatcaga tcgtcggcct tgccgttgcc gtcgtcgtcg
     3601 aggccgttca gatctccggc atcgtaaatc caggcgctgc cgttatagta ggtggtatag
     3661 ccgttggagt tggcgtcctc tcccaggttg ttccagaggt tggcacccag gtcggcatgg
     3721 ctccatctca ccccggtgtc catcacggcc aggatcaccg gctgggctcc gtcttcgcct
     3781 ttatgaacgt cccaggccgc ttctgccatg aggggcacaa agtatagggc ggaaggatag
     3841 ttggggtcgt tgggaacggc cagcacggca tctatataga cgggctcggc atattcgacg
     3901 cgggggtcca gactcaagga gttgatcaca gccagaggat ggacggaggg ggaaaagcgg
     3961 gcttccagga tgaggctgag gtctggagct ccggctggtt tttgggcatg atcatagcgg
     4021 aagcggggct tgacagagtt gactcccaag ccttgcagcc gcgcgtccag ctccgatata
     4081 cctgtcagga ctgctcctga agagagagcg gatttgtatt ccggcctcag cttgaactgg
     4141 atcgtgccag cctcgatggg gtttccagcc gtatcggtca gttctgtcag tccggcgttt
     4201 aacacactca aagatatgca aataataagc agcgtcacac taagcttggt catcatcttg
     4261 ctcctgtttg ggggattgac acaggggtct cgcggtaaag acagtatagc atagtatcca
     4321 cctttctcaa aacataatcc ttatcagacc ggttaagcat agaaaatact tgacctctaa
     4381 cccgcatccg cgtttcttca ctccatcaag caaaaaggag ctgatcatga tcattaccac
     4441 cactcccaac attgatggtt acaaggtaga tcactaccat ggcatcgtaa ccggggaagc
     4501 catcatgggc gccaatatcg tgcgcgatct catggcctcg atcacagaca tcgtgggcgg
     4561 ccgcagcaaa gcctatgaat ccaagctgga agacggacgc aatgaggcct tccgggaaat
     4621 gaccgccaaa gcggaacgcc tgaacgccaa cgccatcctg ggcgtcgata tcgactacga
     4681 agtgatcggc cagagcatgc tgatggtggc cgtttcgggt accgccgtga cggtaagcct
     4741 caaggagagc tgaggagatt gattttgcta tcgcggagca ggacagagat actgctgatc
     4801 cagccctaca gcaccttcct cagcacccgg aacccgatgt tgatatctat gtggctggcg
     4861 aaataatacc agcggaaatc aacctccacg ataccggcaa agttgaccca actgccacca
     4921 cggatgatcc ggtgatcgcc atccaggggc ccggtgggat tggtctgggg agtgtttggc
     4981 aaagcgccat agatatccca gcaccattcc cagacgttgc cggtcatgtc gtagaagccc
     5041 agttcgttag gcagtttcag gcccacggga tggtttccgt agtccggatg gccctggctg
     5101 taggatccgg aattgtcata ataccagccc acctccccgg ggatattgct gccgctgtaa
     5161 atgtaatcgt tgcttaggtt tccgccccgg gcggcaaatt cccactccat atcggtgggc
     5221 agccggtaac cggatgcgtt aaagtcgcag ctaacatact gatgattgcc gtaatccagg
     5281 tcccaaccgg ccggccagtt atcgggatcg ctgccataga cgctgaattg ataacaggga
     5341 gtcaggcctt cctgcaagct gcgccggttg cagtattcca aggctttgaa ccaggaaacc
     5401 tggtaaaccg gatagctgtc tcccacccca tatccccagg aggggcttga gcccatcacc
     5461 tgctgataag ctagctgagt aagctcatat tgggcaaggt aatagctgcc gagggtgacg
     5521 gtggaatagc cgttgtaaaa gctgcctccc tccacgagga taaaacctgg aggcggagga
     5581 gtggtaaatc catcatcggc atagacgcgg taaaaatagc ccccatccag ttcaaaatct
     5641 tcccctccgg cgtcccagac gatgtgtttg cccggacccg gagcaatatc tgctcccaca
     5701 tccccgctca tcctgaccgc actggccacg atatcgaagc tggtaccccc gtttgagctc
     5761 aatcttagcg acaccgcgca ggaatcgcct tccgggtcat tcaggtcata ggtgatatcc
     5821 acgatcttgc tgccatcgag acgctgactg gcagagacgt tacttaccac cggagagagg
     5881 ttgcgggcga tggattccgc tcctgaaagc tgccagggca ggcagatcaa gaccaggagg
     5941 aagcagagag gcagcacaag gatagtcctc atttcaggcc tcccttccat cctctcaagc
     6001 aggttctcag ctctgccagg ctgattctat ctcccggctt caggctggcc agcagcgtgg
     6061 ccattctctg gtcatagtcc ttgtaggcga tcactttata aaacatgctg tcccgaaagc
     6121 ggaccacccg cgggtgggtg tgttccagct ggtcggtctc tgccagaaag tagaaatagg
     6181 ggtcgtcagt gctgggagtc tcgttgtaga ggacgatgta gccgtccggg acaatgggat
     6241 agttgtaggg aagaatggtg tgggagaccg catcccaggt gatcacggca tcctcatcat
     6301 tggagatgtc cactctcagg ttttgaggtt cggcaggcgg cacatagctg atgctgaagc
     6361 tctgctggct ggattcgcgg gtaaaattgc caaagtaatc cgccaccctg attctcactc
     6421 tggcggcctc agtctgatag gccggagcgg tccaggaatg ggtaccatcg ttggcagtgc
     6481 cttccgcgat ggggatatac ccggtcccgc cgttgaggct gtaccagatg taaacgctgt
     6541 tggggatgag gttggaatcc gaggcgttcc actggatctg atacgtctca ccaataaacc
     6601 agttttctcc gccattgggc gcgacaatac ccagctcagg actgactgtg tcgttgatca
     6661 caagcgctga tgtgccatga cctgtaccag cgccaagcga gagcataaac gcgccgcaca
     6721 aaaggcatag atagaccttg atcatgggtc ctcccctgga aaagaatgat gggagcatct
     6781 tatgatcgcg ctatcaagcg tcaagtgaaa tgtcctagat ttgcccaatt gggtttacaa
     6841 gccactaatc ccaaagtttt ttatcaggtg cctctagaag tactttgagc atcagtggga
     6901 ggattttaag attgacagat aatcataggt gaaaaaccat caaacaagtg ttgaattgc
//
LOCUS       DAOSFN010000132         6895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_9570_length_6895_cov_9.764327, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000132 DAOSFN010000000
VERSION     DAOSFN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9570_length_6895_cov_9.764327"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1750)
                     /locus_tag="PLO35_08130"
     CDS             complement(92..1750)
                     /locus_tag="PLO35_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79678.1"
                     /translation="MHRIIIIFLLAIIATSTMARVHEWEALQADLGRQAAAFRNSPEH
                     LEARNRDTRDYQVGDTNIFWRWNLSVMPPLWIQTAATCRAVGEHCYIFVADNEWNVHM
                     DQTDVDAVLARLEDSTVNDPSQGAIEMDVQLFGPIPDELDNDPRLIVFYSALGSFQGT
                     SFDGYFSPYNQVTEAQAQQMNPPGHSNLCEMIYMTCYPLDPVAPIRLSVLAHELQHLI
                     HWGQDANEDTWLNEGCSELAMVAYGVPDPISGFNSVPDNNLTAWNQTTADYVKVMLFF
                     TYLQEHYDSTGMIGDLVADPANGIASLNAQLAIHYPDTSFSDLFSNWGVANILDAGPP
                     LNPLWGYSLLDLPNFTMTNLPVEPSMSNLGIQGYATDYLRYTCQTSANRHIGINIGTP
                     IRIKEVKLASNGECLDVTDLGESNSFLVTPPPSSEAFHVIYVLSNPNPNPVVYSLMTA
                     VPNHDPSAPELESLSLSANHPNPFRAQTLIQFSIREAQALKLEVFNLRGQKVALLVDE
                     LKAAGDHQVSWNGQDFEGKKVPAGVYLYRLTAGKTQITRRMAMY"
     gene            complement(2198..2671)
                     /locus_tag="PLO35_08135"
     CDS             complement(2198..2671)
                     /locus_tag="PLO35_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79679.1"
                     /translation="MKLRHLILPLVLIMVLISGCERPVKAKEHKAISAQIDSFMVELF
                     TDAEKLQTKALENYLDEGPSARFFMGAKLYRKTTLLKEIKRAYEGFTSQKLVVMDPKA
                     IVVSRNAVVWTAQVSSLAIGKDGVEYPNSLSETWLWQKKGEYWTVTHFSEAWVNK"
     gene            complement(2859..4835)
                     /locus_tag="PLO35_08140"
     CDS             complement(2859..4835)
                     /locus_tag="PLO35_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79680.1"
                     /translation="MLRKLLLAGLFCLVLIPVLRSEDAYKVMEQVRFLKSWLDINPKL
                     EDVIDPQISPQQLEERKEEIRGGIRRLLASSSEPRKTTKIKVLGYSNQRGALSVDNLD
                     LNQHYYFAMPYRQAEGIIKNPKTWYLTAIRELNWELEWIYKDWKLQGAEINYAQQPEP
                     VYTIPPRLPIEYFTEPQPDINERLLSSEANAYHAYQRFQAGDYPASLNGFEQLIQASP
                     EPNPVYYYWLGRSFLALDNVNEGQTWLKLYLDSGDTSYTTSAKDLMEIINRQYHIFRS
                     VDKAALPRELVTNEGESRYTVHPVDGSLWFSSARPADFNGSNLWRAEKTDTGWANPML
                     VKELCSNADEAPGSFSPDGKTLWIAGKYDSGRRDFDILSSSATDSLWTTPLMLRDLNS
                     SYDDIDPLLIGDKVLIFSSSRPEGQGGFDLWYSELKDSLWQTPVNLGPAINTAGDEDS
                     PWLSWDGKTLYFSSNGYPGLGGHDIYKTVKLQSGWQSWSLPENLGPVINTPARERGFL
                     QLKDTNQAIRVSERAGYPYGEYLVLDYAPRSYWSVNEQGYAVWQQDQDKTSPLTGTVR
                     IEAIAEPKFYKLTISVTASTGQQLQPELKLRYLIDGISYLQTARPNQLGLYETELPLA
                     EVYTVECSLSGYKDYRLEHRPGAESSRQLDVVME"
     gene            complement(4849..6855)
                     /locus_tag="PLO35_08145"
     CDS             complement(4849..6855)
                     /locus_tag="PLO35_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79681.1"
                     /translation="MKRILIIALLFSTLGLGILGAQEISGFQASFVDKRYEISFSIPG
                     GSASGRYDLFIYTRLVGSEAWAKASTILGDFRNLPAFHNGKIFWDPFFDYRKEGNYEF
                     RLYAVPLQYVKSDYNYTKVTQVGMLRVFSDQSAATYRLNGKDILSTDRPALPVGDYEV
                     SLLQNGVVIETRTVSILPFQYTETDLSPRFGNLTLSCNLSGALYAIGDGSFSDQASHR
                     LKTGTYKVKVNIPQSNAAYPSLSAQADVVVEHGRENTYRFEVPYGTLSLSADLDRVSY
                     QLLGKDFQDIQGMMLSPGSVEVRAISDDNTARITQIMNRNLTITNGATTTHSFNFKDA
                     WGFLSVSTPSPASFVTVNGFKLTPGDAFKVPEGIYKVTAVMPDGKIFGPTDVRVQPGQ
                     ISVHNFGVDPDVNLDVVKVPKSSKKSKTEIFKWPLSDISLLGVLSDANRFPNPDAPSD
                     SLKPNKNQQDLALGISAVGVIETKMMRNMGNLHYFWSVGVLDQLYFMMDTRSNKTSFA
                     TDAVSLGGGAGVKAFGERMYFELSFKGSLSTQTPLYRTLTLFNPADTTNVKYRYAVSL
                     KVDRGDKTIHTANNLEWGGAFQAKAKLQYRFSKLANFYAFAAWRLQTENSGKWYRDDE
                     LTAWENDGSLPKPATVSDLRFPTRNVIFSGESLIFGLGFSLVAW"
BASE COUNT         1413 a         1855 c         1982 g         1645 t
ORIGIN      
        1 cacgataaaa gtatgcgaga cagcgagttt gcgggccgct tggacttcaa ccagcgcgcc
       61 cgcgagatga gtctgtctgc tcccgagggg cctaatacat cgccatcctg cgggtgatct
      121 gagttttgcc ggctgtcaga cgatagagat acactccggc cggtactttc ttgccctcga
      181 aatcctggcc gttccagctc acctgatggt ctccggcggc cttaagttca tccaccagaa
      241 gggccacctt ctggccgcgc aggttgaaga cttccagctt gagggcttgc gcttcccgga
      301 tggaaaactg gatcagcgtc tgggctcgga agggattggg gtggttggca ctcaggctga
      361 gagattccaa ttccggggcg cttggatcgt ggtttggcac ggctgtcatt aatgaataga
      421 ccacggggtt gggattcggg ttggaaagca cgtagatcac gtggaaggct tcgctgctgg
      481 ggggaggggt caccaaaaag gagttgcttt ctcccaggtc cgtcacatcc aggcattcac
      541 catttgaggc cagctttact tctttgatcc ggatgggcgt cccgatattg attccgatat
      601 ggcggttggc gctcgtctgg caggtgtatc tcagatagtc tgtggcatag ccctgaatcc
      661 ccagattgct catggaaggc tccaccggca ggttggtcat ggtgaaattc ggcaggtcca
      721 gcaagctata gccccacaag ggattcaggg gcggaccggc gtccagaatg ttggccacgc
      781 cccaattgct aaagaggtcg gagaagctgg tatcaggata gtggatcgcc agttgggcat
      841 ttagagaggc aatgccgttg gccggatcgg ctaccaggtc gcctatcatg cccgtggagt
      901 cgtaatgctc ctgcagatag gtgaaaaaca acatcacttt cacataatcc gccgtggtct
      961 ggttccaggc ggtgaggttg ttatccggaa cgctgttgaa cccgctgatg gggtccggaa
     1021 cgccgtatgc caccatcgcc agttccgagc aaccctcgtt gagccaggtg tcttcattgg
     1081 catcctggcc ccagtggatg aggtgctgca gctcgtgggc caatacggaa agcctgatcg
     1141 gggccacggg atccaaagga tagcaggtca tgtagatcat ctcgcacaga ttgctgtgac
     1201 cgggagggtt catctgctgg gcctgggctt cggtaacttg gttatagggg ctgaaatagc
     1261 catcgaaact tgtcccctga aacgaaccca gcgctgaata gaacacgatc aagcggggat
     1321 cgttgtccag ctcgtccggg atggggccaa aaagctggac gtccatttcg atggctccct
     1381 ggctgggatc gttcaccgtg ctgtcttcca gacgggccag caccgcgtcc acgtccgtct
     1441 ggtccatgtg gacgttccat tcgttatctg ccacaaagat atagcagtgc tctcccaccg
     1501 ctctgcaggt ggccgcagtc tgaatccaga ggggtggcat cacggaaaga ttccagcgcc
     1561 agaagatatt agtgtcgcct acctggtaat ctctggtatc cctgtttctg gcttccaggt
     1621 gctcgggcga gtttctgaaa gcggctgcct ggcgacccag atcggcttgc aaagcctccc
     1681 attcatggac cctggccatg gtgctggtgg caataatcgc cagcaggaaa atgatgataa
     1741 ttctatgcat gagacacatc cttctataca ttataaagac cgttgcgcat aaacgtaacg
     1801 ctggctttag ccggccgtag cactgacttt agtcggtgcg tggcgataac ctatttggct
     1861 gtatggctat taattagaga ttcctggctg tccgtgtgag gtgccggcta aagaccgtca
     1921 ctacgaccgg ctgaagagct tgtgaaaaaa taccaccttg gccgtcttgg agttcaaagc
     1981 ccctaaatag attatacgtt tatctctata cctgacgggg ctttggactt acgtgcagca
     2041 catcgaagac atttatctcg cgggtgtact ggttgaagtc caagcggccc gtaaactcgt
     2101 tgtcatgcac acatttatcg tggccgcttg aactccaacg ggactgaggt gccggcgaaa
     2161 gccagtgata caactctaaa acctcacaac tctacaacta cttattcacc cacgcttcgc
     2221 tgaagtgggt cacggtccag tattcacctt tcttttgcca gagccaggtc tcggagaggg
     2281 aattgggata ttccactccg tctttgccga tggccaggct ggaaacctgg gccgtccaga
     2341 ccaccgcgtt gcggctgacg acgatggcct tggggtccat caccaccagt ttctgggaag
     2401 tgaagccctc gtaggcgcgt ttgatctctt tgagcagggt ggtcttgcga tacagcttgg
     2461 cacccatgaa gaatctcgct gaggggcctt cgtccagata gttttccagg gctttggtct
     2521 gcagcttttc ggcatcggta aagagctcca ccataaagct gtcgatctga gcgctgatgg
     2581 ctttatgttc tttggccttg acgggccgct cgcagccact gatcaaaacc atgatcagga
     2641 ccagcggcag aatcagatgt ctgagtttca tgttggttcc ttaggaggtt gtatggtttt
     2701 agggttgaag ggttttagag ttttagagtt ttgaggtttt cgagttttga agttgatctc
     2761 acatgcgcag ccatctcacc tacgcagaat ctcacctggc gcagccatct cacctacgca
     2821 gtatctcacc tggcgcgcca tctcacctgc gaagcatctc actccatcac cacatccagc
     2881 tgcctggaac tctcggctcc cggtctgtgc tccagcctgt agtccttata accgctgagg
     2941 ctgcattcca ccgtataaac ttccgccaga ggcagttctg tctcgtagag gcccagttga
     3001 ttgggccggg cggtctggag ataggaaatc ccgtcgatga ggtagcgaag cttgagctcg
     3061 ggttgcaatt gctggcctgt ggacgcagtg acgctgatcg tgagtttgta gaatttgggc
     3121 tcggcgatcg cttcgatcct cacggtcccg gtcaggggcg aggtcttgtc ctgatcttgc
     3181 tgccagacgg catagccctg ctcattgacg ctccagtaag accggggcgc gtaatcaaga
     3241 accagatatt cgccatacgg atagccagcg cgctcgctga cccggatggc ctgattggta
     3301 tccttgagct gcaggaaacc acgttcccgg gcgggagtgt tgatcaccgg gcccaggttt
     3361 tcaggcagcg accagctttg ccaaccgctc tgcagcttta cagtcttata gatatcgtga
     3421 ccgcccaggc cagggtagcc gttggagctg aaatacagcg tcttgccatc ccagctcagc
     3481 cagggtgaat cttcatcccc ggccgtgttg atggccggcc ccaggttgac gggcgtctgc
     3541 caaagactgt ctttgagctc gctataccag aggtcaaagc cgccctgtcc ttccggacgg
     3601 gaagagctga agatcagcac cttatcgcca atcaagagag gatcgatgtc gtcatagctg
     3661 ctgttgagat ctctgagcat gagaggcgtg gtccagaggc tgtccgtggc gctgctgctc
     3721 aggatgtcaa agtcccggcg gccgctgtcg tattttcctg ctatccagag cgtttttccg
     3781 tccggtgaaa aggaaccggg agcttcatcg gcattggagc ataattcttt caccagcata
     3841 gggttggccc agccggtatc ggtcttttcc gctctccaga ggttggagcc gttgaaatct
     3901 gccgggcggg cggatgaaaa ccacagactg ccatccacgg gatggaccgt gtagcggctt
     3961 tcgccttcgt tggtgaccag ttctcgtggc agtgccgctt tatccacact gcggaagatg
     4021 tggtactggc ggttgatgat ctccatcagg tctttggcag aggttgtgta actggtatca
     4081 ccgctgtcca gatagagctt cagccaggtc tgtccctcgt tcacgttgtc cagtgccaga
     4141 aaactgcgac ccagccagta ataatacacc ggattgggct ccgggcttgc ctgaatgagc
     4201 tgttcaaagc cgttgaggga ggcggggtaa tcgccagcct ggaaacgctg ataagcatga
     4261 taggcattgg cctctgagct taacagacgc tcgttgatat ccggttgcgg ttccgtgaaa
     4321 tactcgatcg gcaagcgggg cgggatagtg taaaccggtt ccggttgctg ggcatagttg
     4381 atctccgctc cctgcagctt ccagtcctta tagatccatt ccagttccca gttgagctcc
     4441 cggatggcgg tcagatacca ggttttgggg tttttgatga tgccttcggc ctggcgatag
     4501 ggcatcgcga aatagtaatg ttgattcaga tcgaggttgt ccacggagag ggcgccgcgc
     4561 tggttggaat agcccaacac cttgattttg gtggttttgc gcggctcgct ggaactggcc
     4621 agcagccttc tgatcccacc ccggatctcc tctttgcgct cttccagctg ctgaggactg
     4681 atctggggat cgatgacatc ctccagcttg ggattgatat ccagccagga ttttagaaag
     4741 cggacctgct ccatcacctt gtaggcgtcc tccgatctca ggaccgggat cagcaccagg
     4801 cagaacagtc ccgccagcag tagcttgcgt agcatcggca gtcctttatt accaggctac
     4861 caggctgaag cccaggccaa agatgaggct ttcaccactg aagatcacgt tgcgggtggg
     4921 gaaacgaagg tccgaaacag tagcgggttt gggcaggctt ccatcgtttt cccaggcagt
     4981 gagttcgtcg tcacgatacc atttgccgct gttttcggtc tgcagtctcc aggccgcgaa
     5041 ggcatagaaa ttggccagct tggaaaagcg gtattgcagc ttggccttgg cctgaaaagc
     5101 tcctccccac tccagattgt tggcggtgtg gatggtcttg tcgccccggt ccaccttgag
     5161 gctcacggca tagcggtatt tcacattcgt tgtatcggcc gggttaaaca gtgtcagagt
     5221 gcggtagagc ggggtctggg tgctcagcga gcctttgaag ctgagctcaa agtacatcct
     5281 ttccccaaaa gctttcactc ccgctccgcc tcccaaagaa acggcatcgg tggcaaagct
     5341 ggtcttgttg gaacgggtgt ccatcataaa atagagctgg tccagcacgc caacgctcca
     5401 gaagtagtgc agattgccca tgtttctcat catcttggtc tcgatcacgc ccacggcact
     5461 gatgcccaac gcgaggtcct gctggttctt attgggtttg agcgaatcgc tgggagcgtc
     5521 cggattggga aaacggttgg cgtcggagag cacacccagg aggctgatat ccgaaagcgg
     5581 ccacttgaag atttccgtct tgctcttttt ggaggacttg ggcaccttca ccacgtccag
     5641 attgacgtcc gggtccactc caaagttgtg aacgctgatc tggccgggct gcactctcac
     5701 atcggtggga ccaaagatct tgccgtccgg catcacggcc gtcactttgt agatcccctc
     5761 cggcacctta aaagcatcgc cgggagtgag tttgaagccg ttgacggtca cgaagctggc
     5821 cggactggga gtgctgacgc tcaaaaagcc ccaggcgtct ttgaagttaa agctgtgggt
     5881 ggtggtggcc ccgttggtga tcgtgaggtt gcggttcatg atctgcgtga tccgggccgt
     5941 gttgtcatcg ctgatggccc tcacttccac gctcccgggg ctcagcatca tgccctggat
     6001 gtcctgaaag tctttgccca atagctgata gctcactctg tccagatcgg cgctgaggct
     6061 cagagtgcca tagggaactt caaagcgata ggtgttttcg cggccatgct ccaccaccac
     6121 gtccgcctga gcgctgaggg atggataggc ggcgttgctt tgggggatat tgaccttcac
     6181 cttgtaggtg ccggtcttga gacgatgact ggcctgatcg ctgaaggagc cgtctccgat
     6241 ggcatagagc gctccgctca ggttgcaact cagggtaaga ttgccaaagc ggggggaaag
     6301 gtccgtctcg gtatattgga aaggcaggat gctcacggtg cgggtttcga tcaccacgcc
     6361 gttttgcagc aggctcacct cataatctcc aaccggcagc gcgggacggt ccgtgctcag
     6421 aatgtctttg ccgttcagac ggtaggtggc cgcgctctga tcgctgaaca ctctcagcat
     6481 gcccacctgg gtcaccttag tgtagttgta gtcgctcttc acatactgca acggcaccgc
     6541 gtagagccgg aactcgtaat tgccttcctt gcgatagtcg aagaaggggt cccaaaagat
     6601 cttgccgttg tgaaaggccg gcagattgcg aaaatccccc aaaatggtgc tggctttggc
     6661 ccaggcctcc gagcccacca gacgcgtgta gataaacagg tcataccttc cggaagcgga
     6721 acccccgggg atcgaaaaac taatctcgta acgcttgtcc acaaagctgg cctgaaaacc
     6781 gctgatctcc tgagcaccca aaatgcccag tcccagcgtt gaaaacagca gagctatgat
     6841 caagattctc ttcatcggct tgactccttc ttgtctgtct ctcttgtgag gttgt
//
LOCUS       DAOSFN010000133         6845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_9666_length_6845_cov_6.879381, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000133 DAOSFN010000000
VERSION     DAOSFN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9666_length_6845_cov_6.879381"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1324)
                     /locus_tag="PLO35_08150"
     CDS             complement(59..1324)
                     /locus_tag="PLO35_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOH79682.1"
                     /translation="MNPKRVLVLLILISSLSVSLFAGGFALSGVGSRATAMGGAYRGL
                     ADDATAMYWNPAGLAFQDGNKVDLGGTFIMPSAKWENPGTNPLTFNSGETESEKALKA
                     FPSAFLTMAKHPKLKYGLGVFVPYGLGSTWDLYASPAGWTTFPENEMSSSIAVIDAHP
                     SVAYKIMPNLSVGAGLGFMYGTIDLAKIGASADTSYVLATTDISGKGMGFGANIGLMF
                     KPTVCMSLGLTAKLPASIKMEGDTKVMNYGLIAPSGPAFDTETTLKLPAEIGIGLSST
                     RIPNLTLNLDYAYTMWSRLDKVELELTGAPASELLFDWKDTSRVSLGAEYQLACNNLR
                     LGAYWDQSPIPEETQTPTLSDIGNKISANIGWGRKFGKLGVDANFQYVTFSEREVDTM
                     NVVGGAPTNILGTYNSQSLSGNLGLSWEF"
     gene            complement(1477..2949)
                     /gene="dacB"
                     /locus_tag="PLO35_08155"
     CDS             complement(1477..2949)
                     /gene="dacB"
                     /locus_tag="PLO35_08155"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOH79683.1"
                     /translation="MRYTYARRSFQRLAAILVLLSCVSWQLLAKEALTQKQLITELDH
                     HRGSSLVKGARLGLGIWDVESGKLIYSHQDSLLMAPASTQKLFTAVAAFEQLGPDFRW
                     QTTIGYRGQIVEGTLKGDLIVIGSGDPTWHEDFWPEGGEQLLALWADSLKTRGISTIE
                     GNLVANVSHFPTKAWNPAWDLSDKPYGYAPAVSSLSLNANRVTFNLKGGKTSGKKVAV
                     SSTDSYNYITLSNALSTSKKSKAANTWAVPTKDAHKFIIKGSVPAGGKAQEKIGVREP
                     EYYCMLALKDVMQKKGITLKGKILSDSKLPDPDSLQVLFNHLSPPLSQVCKVMLKTSS
                     NFIAESLLHSLDRDYKLAIGKVLTALDSLHVSTRGMKIQDGNGLSRTALCSPLQMGTM
                     LCEITHQDWFEHFFGSLSIAGIDGTLQKRFSKSPARGSVFAKTGSMSGISNLAGYLKT
                     KDGRLLAFVAYGSKLKSVGNARKWQEALCDIVYRYIDTDQ"
     gene            3225..>6845
                     /locus_tag="PLO35_08160"
     CDS             3225..>6845
                     /locus_tag="PLO35_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH79684.1"
                     /translation="MNLIRLFAKALLVSLAVCIILPAPLEAVPLSGNYSVGGVGANFA
                     TLSAALNAAQTEGLSGTTNLILNAGTYSGPFEINLPANNHTLMVRAAENATVILNNPD
                     ASSAQNYIFYINSTPKVYLQDLRFEASGSYARGLWVHGDSDDLSVVSCTFVNTDSANS
                     SEAIYVTADGSNDADDVLIALNSFQYGYAHIYTNTYHGATNFSGWELYLNHHNSAYYG
                     VYIKQASDLEINALITQNVNSAVVLDQCSGNLDMQRNNISAYQTGVYVYNSSFSLSSP
                     GVVNNVFRISGRGWYNPNYDVDSQGIYIAASENLKVYHNSVRNTSRASGSYSLGVSGT
                     GNLIRKNILASTGSGYAIYAWSLSGNTIEYNNFWSSHSSFARIQNDWITDLEAFNTLT
                     GTGNLAYYPFFTDDQLRTVAPKLDNYGPSYGITTDFNNAPRDATSPDIGAHEFTSDPA
                     MTPLSGTINVGVGQSYTTLQQFFTALSFRGVSGLTTAQLTDTLYVEQVQVFGIPGSNP
                     SYLVQVKGHATLGSTLRWDNQTAADNFVMRLKRMTHIDFHDIRFQTASLQYANLVWMP
                     GYNYKVRFLDCMFKAPVGAQGNGIMSEYQDNNLDLSVLSCGFYNNRLGLQHSGDLLSV
                     GSSWFMGQYQGISLTSSNRCEITGNYFENHSNAAVITNGGNRLLVSHNRIKGTGTGLA
                     LYNLSLFENQRNLIYNNRIHVTGGNQSSGVTLGANGINLLNNSILCQSTNSAGLYIYQ
                     NGSQTDVVNNIFQAASGLAMEIIYYTPSADFVIDHNDYFSLGTYIVRHGSSYYASLAA
                     LGSGDPANNLHSLNVDPHFTEDLHSTSPWLRQAGTMRAEFDDDIDFELRGTQWDIGAD
                     QQTGPLGFSPLAGTYTVGQGGNYPSIEAFLDALHLYGTSGNVTANLLAGIYPGYNVIG
                     NFPRVIPGSPLTINALPGASFNLIPSNTYNYENFIFRLDGADTIVFQNIGFGVESSSR
                     QSILILLQGKCDEISLLDCGFNMGSQSSTAIQATSSIASGLLISGCNFTGGSYGVYLS
                     GVSSSAGYNDIRVQHCTMQGTYNPLTISYASGLKLTSNTFADFSATPSLSYIWGNSDI
                     LRNKFRAHNFVGFFSSAQMLSLNSLTGTEEQPIEIIANLIYSRGNQVLSVTGISIGNS
                     SWLRLIHNSISVENFYSFEYGSALQMSSCANVWSANNIFSNPGTGLSLNLSQCSALSF
                     NNN"
BASE COUNT         1586 a         1907 c         1784 g         1568 t
ORIGIN      
        1 gatacaaaaa aacggcctca ttactgaggc cgccagatta catgatcagg tgtttagctt
       61 agaattccca gctcaggcca aggtttccgg agagggattg ggagttgtag gtgcccagaa
      121 tgttggtggg agctccacca acaacattca tggtgtcaac ttctctttcg ctgaaggtca
      181 catactggaa attggcgtcc acacccagtt tgccaaactt acgtccccag ccgatattag
      241 cgctgatttt gttgccaatg tcgctaaggg tgggagtttg ggtctcttcg ggaatgggag
      301 actgatccca ataagcgccc agacggaggt tgttgcaggc cagttgatat tccgcgccaa
      361 ggctcacgcg ggaagtatcc ttccagtcga agagcagttc agaggctggc gcgccagtca
      421 gttcgagctc gaccttatcc agtctgctcc acatcgtgta agcataatcc agattgaggg
      481 tgagattggg gatacgggtg ctggataagc caataccgat ctcagcggga agcttgaggg
      541 tggtttcggt atcaaatgca ggaccggaag gggcaatcaa tccgtagttc atcactttgg
      601 tgtcgccttc catcttgatg ctggctggca gtttggctgt caaacccagg gacatgcaga
      661 cggtgggttt aaacatcaaa ccaatgttag cgccaaaccc cattcccttg ccggaaatgt
      721 ccgtagtggc taatacatag ctagtatcag cgctagcacc gatcttggcc aggtcgatgg
      781 ttccgtacat aaagccaagt cctgctccga cagagagatt gggcatgatc ttgtaggcca
      841 cgctgggatg cgcgtcgatc acggcaatgc tgctgctcat ctcgttttcc gggaatgtgg
      901 tccaacccgc ggggctggca taaagatccc aggtactacc aagaccgtag gggacgaaca
      961 cgccaagacc atacttgagc ttgggatgtt tggccatggt caggaaagcg ctgggaaacg
     1021 ctttaagagc cttttcggat tctgtttcgc cggagttgaa tgtgaggggg ttggtaccgg
     1081 gattttccca tttggcggac ggcatgataa aggtgccgcc cagatcgact ttgttcccat
     1141 cctggaaggc cagaccggct ggattccagt acatagcggt agcgtcatcg gccaggcctc
     1201 tgtaagctcc gcccatagcg gtggcgcggg aaccgacacc ggaaagcgcg aagcctccgg
     1261 caaagagaga tacgctgagt gagctgatca gaatcagcag cacaagcaca cgttttggat
     1321 tcatgtgtgt tctccttttg ttgaaatggg ttcaaagatt atgagtccta aagatgaatg
     1381 atattccaaa gcggtgtatc tgtcaataag aaaatacagg acaaagtctt acaaccccca
     1441 aagctgccct ctccaaattc accagggagc cataaactac tgatcagtat ctatatagcg
     1501 atatacgata tcacatagtg cttcctgcca ttttctggca ttgcccacgc ttttgagttt
     1561 actgccgtaa gccacaaaag ccagcaaacg accatctttg gtcttcagat aaccggccag
     1621 attgcttatc ccgctcatag agccggtctt agcaaatacg gaaccccggg cgggggattt
     1681 tgagaagcgt ttctgcaggg tgccatcgat tccggcgatg gacagagagc cgaagaagtg
     1741 ttcaaaccag tcctgatggg tgatttcgca gagcatggtt cccatctgga gaggactgca
     1801 gagggcggtc ctgctgaggc cattgccgtc ttggatcttc atgccgcggg tggagacgtg
     1861 cagactgtcc aaagcagtca agaccttgcc aatcgccagt ttataatccc ggtccaagct
     1921 gtggagcaag ctttcggcga tgaaattgga gctggttttc agcatcactt tacacacctg
     1981 gctcaagggg ggagagagat ggttaaacag gacctgaagg ctgtccgggt ctggcagctt
     2041 gctgtcagac aggatcttgc ctttcagggt gatccccttt ttctgcatca cgtctttgag
     2101 ggccagcatg caatagtatt ccggttcccg cacaccaatc ttttcctgag ctttgccgcc
     2161 ggcggggacg ctgcctttga tgatgaactt gtgagcgtct ttggtgggca cggcccaggt
     2221 gttggcggct ttgctctttt tggaggtgct gagagcattg ctcagagtga tgtagttgta
     2281 gctgtccgtg ctggatacgg ccactttttt accgctggtt ttgccgcctt tgaggttgaa
     2341 agtgacgcgg ttggcgttca gcgacagaga actgacggcc ggcgcgtagc catagggctt
     2401 gtcgctgagg tcccaggcag ggttccaggc cttggtggga aagtggctca cgttggccac
     2461 cagattgccc tcgatggtgg aaatgcctct ggtcttgaga gaatcagccc agagggccag
     2521 cagctgttct cctccctccg gccagaaatc ctcatgccag gtgggatcgc cgctgccgat
     2581 cacgatcagg tctcctttca gggtcccttc cacgatctgg ccgcgatagc cgatcgtggt
     2641 ctgccatctg aaatcggggc ccaattgctc aaatgccgcc acggcggtga agagcttctg
     2701 agtggaagcg ggggccatga gcaggctgtc ctggtggctg tagatgagct ttccgctctc
     2761 cacgtcccaa atccccagtc ccaatctggc gcctttgacc aatgaggagc cgcggtgatg
     2821 atccagctcg gtgatcagtt gcttctgtgt aagcgcttct tttgccagca attgccagct
     2881 cacacagctg agcaggacca ggatggccgc caggcgctgg aaagaacgac gtgcatatgt
     2941 ataacgcatc aaaaacctct ctatagcaag ctttgacagg tctgctctcc tgtcaagcac
     3001 ttcatcgcgc tggtctgttt ctcagagcga ttccgggcgg gtgaaagctt gccggcgatg
     3061 cgataagacc ggagtctggt ttttggcttg acatcccagc cctctttcaa acttgtctca
     3121 cagtagttgt gtcggtatta aaaagattaa ggtttctaag atcagacacc cttcccttca
     3181 cagtttgtaa aaaacataag cagctataaa caatggagta gtatatgaat ctgattcggt
     3241 tattcgcgaa ggccttgctc gtcagcctgg cagtgtgtat catccttccc gccccgttgg
     3301 aagcggtgcc cctcagcgga aattatagcg tgggtggagt cggggccaat tttgccactc
     3361 tcagcgcggc cctcaatgcc gctcagacgg aagggctttc cggcacgaca aacctgatcc
     3421 tcaacgccgg cacctattca gggccttttg agatcaacct tcccgccaac aaccacaccc
     3481 tcatggtccg ggccgcggaa aacgcgacgg tgatcctcaa caatcccgat gcgagctcag
     3541 cgcaaaacta cattttctat atcaacagca cccccaaggt ctatctgcag gacctgcgtt
     3601 ttgaagcatc aggatcctat gcccgcggtc tttgggtgca tggagacagc gacgatctaa
     3661 gcgttgtgag ctgcaccttt gtgaatacag acagtgccaa cagctccgag gccatctatg
     3721 tgacggcaga cggatccaac gacgcggacg atgtgctcat cgcgctcaat tcattccaat
     3781 atggctacgc gcatatctac accaacacct atcacggcgc caccaatttc agcggctggg
     3841 agctgtatct aaatcaccac aactcagcct attacggcgt ctatatcaag caggcatccg
     3901 acctggaaat caacgctttg atcacccaaa acgtcaatag cgcggtggtt ctggaccagt
     3961 gcagcggcaa tctggacatg cagcgcaaca acatcagcgc ttatcaaacc ggcgtctatg
     4021 tctataacag cagcttttca ttaagctctc caggcgttgt caacaacgtc ttcagaatca
     4081 gcggccgggg atggtacaat cccaattacg acgtggacag ccagggtatc tacatagccg
     4141 cttcagaaaa ccttaaggta tatcacaaca gcgttcgcaa cacttcccgt gccagcggtt
     4201 cctacagcct gggtgtttcc gggaccggca atctgatccg taaaaacatc ctggccagca
     4261 ccggatcggg ctacgcgatc tacgcctgga gcctgagcgg caacacgata gaatacaaca
     4321 acttctggag cagccacagc tcctttgcca ggattcagaa tgactggatc acagatctgg
     4381 aagccttcaa taccctgacc ggtaccggca acctggccta ctatccattc tttacggatg
     4441 atcaattgcg caccgtggcc cccaagctgg ataactacgg cccttcctat gggatcacga
     4501 cggatttcaa caacgctccg cgcgatgcca caagcccgga tatcggagcg catgaattca
     4561 cttccgatcc tgccatgaca ccgctgtccg ggaccatcaa tgtgggagtg gggcagagct
     4621 ataccaccct tcagcagttc tttaccgctc tcagcttcag aggcgtgagc gggctcacta
     4681 ccgctcagct caccgatacc ctctatgtgg aacaggtgca ggtttttggc attccaggct
     4741 ccaatccatc ttacctggtt caggttaaag gacatgcgac gctgggctcc accctcagat
     4801 gggacaatca gactgccgcg gataactttg tgatgcggct caagcgtatg acccacatag
     4861 actttcacga catccgcttc cagaccgcgt ccctgcagta tgccaatctg gtctggatgc
     4921 cgggatacaa ctataaggta cgctttctgg attgcatgtt caaagctccc gtgggggctc
     4981 agggcaatgg catcatgtcc gagtatcagg acaacaatct ggacctgagc gtgctgagct
     5041 gcggttttta taacaaccgc ttaggcctgc agcacagcgg tgatctgcta tcggtgggca
     5101 gcagctggtt tatgggccag taccagggaa tcagtctcac gtcctcaaac cggtgcgaga
     5161 taaccggcaa ctactttgag aatcacagca atgccgctgt catcaccaat ggcggtaacc
     5221 gtttgctggt aagccacaac cgcatcaagg gcaccggaac cggcctcgcc ctctacaacc
     5281 tcagcctctt tgaaaaccag cgcaacctca tctacaacaa ccgcatccac gtcaccggag
     5341 gaaaccagag cagcggtgtc actttgggtg caaacggcat caacctgctc aacaacagca
     5401 tcttgtgcca atccaccaac agcgccggtc tatacatata tcagaacggc tcccagacgg
     5461 atgtggtcaa caacatcttc caggccgctt ccggtctggc gatggagata atctattaca
     5521 ccccttccgc ggattttgtg atcgatcaca acgactactt cagcctcggc acctatatcg
     5581 tgagacacgg ttcctcctac tatgccagcc tggcagctct ggggagcggt gaccctgcca
     5641 acaacctcca cagcctcaat gtggaccctc attttacgga ggatctgcac agcaccagtc
     5701 cctggttgcg tcaggctgga accatgagag cggaatttga tgacgacatc gatttcgagc
     5761 tccgtggcac tcaatgggat atcggagccg accagcaaac cggtccgctt ggattctctc
     5821 ctctggcagg aacttacacg gtgggccagg gcggtaatta tccgtctata gaggctttcc
     5881 tggatgccct gcatctgtat ggcaccagtg gaaacgtgac ggccaacctt ctggccggca
     5941 tctatcccgg ctacaacgtg atcggcaatt tcccgcgcgt gattcctggc tccccgctca
     6001 cgatcaatgc cttgccgggt gccagcttta acctcattcc ctcaaacact tataactatg
     6061 aaaacttcat cttccgtctg gacggggccg ataccatcgt gtttcaaaac atcggctttg
     6121 gagtggaatc ttccagccgc cagagcatcc tgattctgtt gcagggcaag tgtgacgaga
     6181 tcagcctcct ggactgtggc ttcaacatgg gcagccagtc cagcacggcc attcaggcca
     6241 ccagcagcat cgccagcgga ttgttgatca gcggctgcaa ctttacgggt ggaagctatg
     6301 gcgtgtatct cagtggagtg agcagcagtg ccggctacaa cgatatccgc gttcagcatt
     6361 gcactatgca gggaacctac aatcccctca caatcagcta tgccagtggg ctcaagctga
     6421 cctccaatac cttcgcggat ttcagcgcca cgccctcgct ctcatacatc tggggaaatt
     6481 cagacatcct gcgcaacaag ttccgcgccc acaactttgt agggtttttt tcatcggcgc
     6541 agatgctgag cctgaacagc ctgaccggca cagaggaaca acccatcgag atcatcgcca
     6601 acctcatcta cagccgcgga aaccaggtgc taagcgtgac cgggatctcg attggcaatt
     6661 cgtcctggct gcgtcttatc cacaacagca tctcagtcga aaacttctat tccttcgagt
     6721 atggcagcgc cttgcagatg agcagttgcg ccaacgtctg gagcgccaac aacatcttca
     6781 gcaatcccgg cacgggttta tccctcaatt tgagccagtg ttcggcgctt agcttcaaca
     6841 acaac
//
LOCUS       DAOSFN010000134         6709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_9929_length_6709_cov_10.182597, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000134 DAOSFN010000000
VERSION     DAOSFN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9929_length_6709_cov_10.182597"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1386
                     /locus_tag="PLO35_08165"
     CDS             76..1386
                     /locus_tag="PLO35_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79685.1"
                     /translation="MKNNSVLPYLVLFVLIFVAGCAKKDAGVSEFGTTDLQKLEIRAQ
                     VKSMRIVSFNAKDNFGKAIKDGFSGDVYYQFNDNGFISEETHYYEDGQMDTHLVYKRD
                     DQMRILEINENATQRYSTPRTVFKYDDGGKNIEKTAYEADNSIAWKIMYSYDAGGNLA
                     EEGMYDGDGSLISKNVYTYDSDNNLTDLKKYGGSFELLSATSYKYFEPKKASKSSDVG
                     MIREAVEYDSKEVLQSTKSYQYNELGLVIGETTVSPGNETTKLTYKYEYNTDKDWIKK
                     ITYYNTVPTVIEERQFNVDNNRPAAVTTDEAKAGNDSKLQTEVFNTVYAWNDAHNTTD
                     LAKFGTVYAEQVSLYGTTMTNDKAVALKKQLLTGKNEGYSQSVSDLRMKTLTDGLVQV
                     DFVKSTQFQNTNKTYNAYLELSKSLGAWKITKESDQTTDKNLRH"
     gene            complement(1720..2970)
                     /locus_tag="PLO35_08170"
     CDS             complement(1720..2970)
                     /locus_tag="PLO35_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOH79686.1"
                     /translation="MDLSLRNKKLLGINGLGRIGKLLLWNQIHLRHFDGIVVNCGREV
                     GKSLDDLLQVIETDSSYGSIHRFLFGLIGKQANIRVLDATKPLLEIEGIPVKILRSAR
                     NPKDINWLNEGVGLVVDCTGSFVDPSLSSDSGKPCLRGHLDAGALKVICSAPFKLKSG
                     SSYPTDSKTMIFGINHLEYDSREHHVISAASCTTTGLSHMIKPLLDHEATSRIMTASM
                     STIHAATNTQSILDSVPKADASDLRKTRSVFNNIILSTTGVTKALEQVMPQIRSIGFM
                     ADSVRIPTSTVSLINLNITFHTGLNSLGEPLVNRKLINEVYQKAAQGPQKDLLVYSER
                     QNVSSDLIGSMAAVTIEAHETHTRTGFITLPAEFLSEHGLSADHGVEFPVTHAKIFGW
                     YDNELGSYVNCLSRLANYIEENSF"
     gene            complement(3369..>6709)
                     /locus_tag="PLO35_08175"
     CDS             complement(3369..>6709)
                     /locus_tag="PLO35_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOH79687.1"
                     /translation="LPSFTDIANRLRESVCATCGVTERLHSIFQLLRLEGMEDLRDHL
                     LWDLNRELTGLYRQMPPQQIPGIIDSVYQALESFKDSHLSIVLDCLLSIGKTVISAGD
                     PAHVAHFIQQLNLLPFTGPGEVRIREDWQLDIDKNHIKHIRLCMELIAINPTLCKDLL
                     AYLVINVTRSGIFISDEDLFQKDVSALLNADIEPVFIQLKHLLRQFPVFYSEIGAEGE
                     IRDLSTELDELSQRRDRLIHFLRKQVHTESNNTHIGLCASILDYWTDLDAAKLAGLIP
                     SDVLLYLQEGDELLEHQRDAVNAFLKDSGYSVTELLSLSWQSLERAFSDQGDEFYLKR
                     LRLLCGIHLLLKDKYGLDPYDIVKFLTRYKFFDSKEQNRLRVSLVRRDYDSSIRQLLG
                     YIGKLNNIILNPIPSAGWENIFYKRHIAAGIPSMYGSYREPKLEAMGMVFRIENVVRR
                     LFENTLRQINLNYINGKNLRRIVKVLELYDFAMRQEMLTNDAYSSTVAMLDSAKNIAN
                     CSLEQYMDIFRLLKDSVNEILSEYYYRCYDPELRRKTTACSRGLDTEIEAEGFYRKLL
                     TMSFMVQGLDVFITNLLETLSQMKRLFKPEDVVRLMSYDPDKLFVHLHGGNSHVENQV
                     ALGSKAFFLKRLWHFEYPIPPGFVMTTELYRNREVINTHPDISAEFDELLRQNLGKLE
                     RSTRLELGDPQKPLLLSVRSGAPMSLPGAMDTFLNIGLNDQITLLLSQRPNYGWTAWD
                     CYRRLIQSWGMAYGIDRDEFDAVMIHYKQLHNIHQKTQFPADVMRAMCEDYKAVLAAH
                     SIELEQEPFRQLYRAVSHVLDSWNTDRAKVYRRKLQISDEWGTAVIVQKMVIGNISLE
                     SGSGVLFTHAVWHDGPGICLNGDYTICSQGEDVVAGLVHTLPISEEQAKAQARPGELS
                     LETSFPAVYNRLLRLSRQLIDDRNFPHQEIEFTFEGPSEDQLFILQTRNQVIRKVPDY
                     LVLCDGASDLTRIGSGIGIGKGAVSGLIVVCQEDITRLKPLGEPLILVRPDTVPDDME
                     LLFECQGLLTARGGVTSHAAVTATRLDLIGIVNCWDLSVDESNSSCQIGSLQLQAGDP
                     ITLDASSGSIYQGRQPLKSIHDVF"
BASE COUNT         1509 a         1633 c         2012 g         1555 t
ORIGIN      
        1 cgccgcgatt cttttaaatt gacaaaaagc gcagggaaat cagtttagaa acaaatgcac
       61 aaatcaggag attacatgaa aaataatagc gtgttaccct atctggtcct gtttgtattg
      121 atttttgtgg ctgggtgcgc aaagaaagat gccggcgttt ctgagtttgg aaccactgat
      181 ctgcaaaaac ttgaaatacg agcgcaagtg aaatctatgc ggattgtcag ttttaatgca
      241 aaggataatt ttggcaaggc gatcaaagac ggcttttccg gagatgttta ttatcagttt
      301 aacgacaacg gtttcatcag cgaggaaacc cattattatg aagatggcca gatggatact
      361 catctcgtgt acaagcggga cgatcagatg aggattctgg agatcaatga aaacgccacg
      421 cagcgatata gcactcccag aaccgtcttc aaatatgacg acggcggcaa aaacatcgaa
      481 aaaaccgcgt atgaagccga taactccata gcctggaaga tcatgtatag ctatgacgca
      541 ggtggtaacc tggcagaaga gggtatgtat gacggagacg gctctctgat cagtaaaaat
      601 gtctatacct acgacagtga caataacctg acggacctca agaaatatgg cggtagtttt
      661 gagctcttat cagccacatc ttacaagtat ttcgagccga agaaagccag caaaagcagt
      721 gatgttggga tgattagaga agctgtggag tatgactcaa aagaggtgct ccaatccacg
      781 aaaagctatc aatacaacga gttggggctg gtaatcgggg aaacaaccgt ctcgcccggg
      841 aatgagacca ccaaactcac ttataagtat gagtataata ctgataagga ttggatcaaa
      901 aaaatcacat attataacac tgttcccaca gtgattgagg aaagacagtt caatgtggat
      961 aacaacaggc ccgcggctgt tactacggat gaggccaaag ccggtaacga ttcaaagctc
     1021 caaacggaag tttttaacac cgtttatgcc tggaatgacg cgcacaatac cacagatctc
     1081 gccaaattcg gcacagttta cgcggaacag gtgtcgctct atggcacaac catgaccaat
     1141 gacaaggctg ttgccctgaa aaaacagctc ctgaccggta agaatgaggg ctactcgcaa
     1201 agtgtgtcag acttaaggat gaaaacactg acagacggtc tggtccaggt tgattttgtc
     1261 aagagcaccc aattccaaaa taccaacaag acgtacaatg cctatctgga gctcagcaaa
     1321 tctctgggtg catggaaaat aaccaaggaa agcgatcaga ccaccgataa aaatctgcgc
     1381 cactgatcag atcctcaagg ggtaagagca cttttttagc catacttgat tttcagccgt
     1441 tcatcttgag aggcttcctg ccgggaataa atgcgcggac caggggtcgg cacgtcccgt
     1501 ggaacggcaa acggggacgt ttaccaatcc gttgccgatc ttctggctgg gtcatcgcat
     1561 tgcgggtgcg atccgcaatg caccagtaat gggatgcggg aaaccctttc atcttctgac
     1621 attctcattg taaaaaaacg cctcccatgg tggcctgtag aatcccgctg acaagcaaag
     1681 cgccagagaa ggtctccggc gctttgttct gggatatcct tagaaactat tctcttctat
     1741 atagttagcc agcctggaga ggcagttgac gtaggagccc agctcgttgt cataccagcc
     1801 aaagattttg gcgtgggtga cagggaattc cactccgtgg tcggcgctca ggccatgttc
     1861 agagaggaat tcggccggca acgtgatgaa accggtgcgg gtgtgggtct cgtgggcttc
     1921 gatggtgacg gcggccatgc tgccaatcag gtcggagctc acgttttggc gctcgctata
     1981 gaccagcagg tctttttggg gtccctgggc ggctttctga tagacctcgt tgatcagttt
     2041 gcggttcacc agcggttcgc cgaggctgtt cagacctgtg tggaaggtga tgttgaggtt
     2101 gatcagcgag accgtggagg tggggatgcg aacgctgtcc gccatgaatc cgatgctgcg
     2161 gatctggggc atcacctgtt ccagggcctt ggtgacaccg gtggtggata agatgatgtt
     2221 gttaaagacg ctgcgggttt tgcgcaggtc cgaggcgtcg gctttgggga cgctgtccag
     2281 gatggactga gtgttggtgg cggcgtggat ggtgctcatc gaagcggtca tgatgcggga
     2341 ggtggcctcg tggtccagca ggggtttgat catgtgggaa agccctgtgg tggtgcagga
     2401 cgcggctgag atgacgtgat gctcgcggga gtcgtattcg aggtggttga tgccaaagat
     2461 catcgtcttg ctgtccgtgg ggtagctgct gccgcttttg agcttgaaag gagcgctgca
     2521 gatcactttc agggccccgg cgtcgaggtg acctctgaga cagggcttgc cgctgtccga
     2581 actgaggctg gggtccacaa agctgcccgt gcagtccacc accagtccca cgccctcgtt
     2641 cagccagttg atgtccttgg ggttgcgggc gctgcggagg atcttgaccg ggatgccctc
     2701 gatctccagg agcggcttgg tggcgtccag gactctgata ttggcctgtt ttccgatcaa
     2761 tccaaacagg aagcggtgga tagagccgta gctgctgtcc gtttcgatca cctggagcag
     2821 gtcgtccagg cttttaccca cttcgcgtcc gcagttgacc acgatcccgt caaagtgtct
     2881 gaggtggatt tgattccaaa gcaagagctt gcctattctg ccgaggccgt tgatgcccag
     2941 cagttttttg tttctcaggc tgaggtccat gtgtctgtct ccttggtgtg tatttttttt
     3001 atcgttaagc gttatgcgtt atgcgttatg cgatccccat gggtacactc gtagcaattt
     3061 ggccccgttg gagttcaagc ggccgaaaca aaagtatgca tgacagcaag tttatgggcc
     3121 gcttggactt caaccagcac gcccagaggc gttgatctcc aagacgggat aaaggtatat
     3181 catattggga gatagcctgg tggtcacgca ccgactgaag tcagtgctac gaccggctaa
     3241 agccagcgtt acgaagtgtc tcatattctc atgtctcatt gtaataagaa aacctctgtt
     3301 ttctctgctt tctccgcttt ctttgaagtt tgtttgcaga gaggttacgc tccagaggta
     3361 ggctgcggtc aaaacacatc gtggatgctc ttgaggggct ggcggccctg atagatggag
     3421 cctgaactgg cgtccagggt gatgggatcg ccggcctgga gctggaggct tccgatctgg
     3481 caggaggagt tggattcgtc cacgctgagg tcccagcagt tgacgatacc gatcaggtcc
     3541 agccgggtgg cggtcaccgc ggcgtgggaa gtgactcctc ctcttgcggt tagcagtccc
     3601 tggcactcga agagcagttc catatcgtcc ggcacggtat ccggtcttac cagaatgagg
     3661 ggctcgccca agggtttcag gcgggtgata tcttcctggc agacaacgat caagccgctc
     3721 acagctccct tgccgatccc gattccggaa ccgatgcggg tgaggtctga agcgccatcg
     3781 cagagcacca gatagtccgg caccttgcgg atcacctggt tgcgggtctg gaggatgaaa
     3841 agctggtctt cggaggggcc ttcaaaggtg aattcgatct cctggtgggg aaagttgcgg
     3901 tcgtcgatga gctgtctgga tagcctcagg aggcggttgt aaacggccgg aaagctggtt
     3961 tccagagaca gctcgccagg ccgggcctga gctttggcct gctcttccga gatcggcaaa
     4021 gtgtgcacca ggccagccac gacgtcctcg ccctgactgc agatggtgta gtctccgttg
     4081 aggcagatgc caggcccgtc atgccagacg gcgtgggtaa agagcactcc ggagccggat
     4141 tccagagaga tattgccgat caccatcttt tgaacgatga cggcggtgcc ccattcatcc
     4201 gagatctgca gcttgcgtct gtaaactttg gcgcgatcgg tgttccagct atccaggacg
     4261 tgggaaacgg cccggtagag ctggcggaag ggttcctgct ccagctcgat ggagtgagcg
     4321 gccaggacgg ctttatagtc ttcgcacatg gcgcgcatca catctgccgg gaactgggtc
     4381 ttctgatgga tgttgtggag ctgtttgtag tggatcatca cggcgtcaaa ttcatcgcgg
     4441 tcaatgccat aggccattcc ccagctctgg atgaggcggc gatagcagtc ccaggccgtc
     4501 cagccatagt tgggccgttg ggacagcagc agagtgatct ggtcgttgag gccgatattg
     4561 aggaaggtgt ccatggcccc gggcaggctc ataggggctc cggatcgcac ggacagcaac
     4621 agcggcttct gagggtcgcc cagttccaga cgggtggagc gctccagctt gccgaggttt
     4681 tgcctcagga gttcgtcaaa ctcggcggaa atatcgggat gggtgttgat gacctcccgg
     4741 ttgcggtaga gctcggtggt catcacaaag ccgggcggga tgggatattc aaagtgccag
     4801 agccgcttga ggaaaaaggc cttggagccc agcgccacct ggttttccac gtggctgttg
     4861 cctccatgca gatgcacgaa cagcttgtcc ggatcatagg acatcaggcg caccacgtcc
     4921 tcaggcttga agagccgctt catctggctg agcgtttcca gcaggttggt gataaagacg
     4981 tccagaccct gcaccataaa ggacatcgtg agcagcttgc gatagaatcc ctccgcttcg
     5041 atctcggtat caagtccacg gctgcaggca gtggtcttgc gcctcagttc cggatcgtag
     5101 cagcggtaat agtactcgct gaggatctcg ttgacgctgt ccttgagcag acgaaagatg
     5161 tccatgtact gctccagact gcagttggcg atgtttttgg cggagtccag catggccacg
     5221 gtggagctgt aggcgtcgtt ggtgagcatt tcctggcgca tggcaaagtc atagagctcc
     5281 agcactttca cgatgcggcg caggtttttg ccgttgatat agttgaggtt gatctgccgg
     5341 agagtgtttt caaagaggcg gcgcaccacg ttttcgatcc tgaacaccat gcccatcgcc
     5401 tccagcttgg gctcacggta gctgccatac atggatggaa tgccggccgc gatgtgacgc
     5461 ttgtaaaaga tgttttccca tccggcgctg gggatggggt tgaggataat gttgttaagc
     5521 ttgcctatgt agcctagcag ctgacggata gagctatcat agtctctcct caccaaagag
     5581 acgcgcaggc ggttttgctc tttggaatca aagaacttgt agcgggtgag gaatttgacg
     5641 atgtcatagg ggtccagccc gtatttgtcc ttgagcaaga gatggattcc gcagaggagg
     5701 cgcagacgtt tgagataaaa ctcgtcgcct tgatcgctga aagcgcgctc caggctctgc
     5761 catgagaggc tgagcagttc cgtcacgctg tatccggagt ccttcagaaa ggcattgacg
     5821 gcatcgcgct gatgttccag cagttcatcc ccctcctgca gatagagcag cacgtccgat
     5881 gggatgaggc ctgccagctt ggcggcgtcc agatcggtcc aatagtccag gatggaggcg
     5941 cagaggccga tgtgggtgtt gttgctctcg gtgtgcacct gtttgcggag gaagtggatg
     6001 aggcggtcgc gacgctggct cagttcatcc agttcggtgc tcagatcgcg gatctctcct
     6061 tcggcgccga tctcgctgta aaagacggga aactgcctca acaggtgctt gagctgaatg
     6121 aagacgggct cgatatcggc attgaggagg gccgaaacat ccttttgaaa gaggtcttcg
     6181 tccgagataa agataccgct cctggtcaca ttgatcacga ggtaggccag caagtctttg
     6241 cagagcgtgg ggttgatcgc gatcagctcc atgcagagcc ggatgtgttt gatgtggttc
     6301 ttgtcgatat cgagttgcca gtcttcccgg atgcggactt cgccgggtcc ggtgaagggc
     6361 agcaaattga gctgctggat aaagtgggcc acgtgagccg gatctccggc cgaaatcacc
     6421 gtcttgccga tgctgagcag gcagtccagc acgatggaga gatggctgtc cttgaagctt
     6481 tcgagggcct gatagacgga atcgataatc ccgggaatct gctgtggagg catctgtctg
     6541 tagagccctg tgagctcgcg gttgaggtcc cagagcaggt gatcgcgcag gtcttccata
     6601 ccttccagcc tcaggagctg gaagatgctg tgcaggcgct ccgtgactcc acaggtggcg
     6661 cagacgcttt ctctgaggcg gttggcgata tccgtgaagg aaggcaggg
//
LOCUS       DAOSFN010000135         6552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_10270_length_6552_cov_6.724488, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000135 DAOSFN010000000
VERSION     DAOSFN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10270_length_6552_cov_6.724488"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1989
                     /locus_tag="PLO35_08180"
     CDS             343..1989
                     /locus_tag="PLO35_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79688.1"
                     /translation="MAHTDEVQNADRPATREPSFIYVFIFLLLLGSFKLRDSALPESS
                     LPRMNILESKAQTAAKIFLDSVWGPDATRGWNARLMYRAFQKVEGDVTPAPELYPKQE
                     IDYLLRHAPAEYVRLAERYKLGALGLVRFSPVSRDRKEAVDVYLSGSYEVYGMILHNW
                     PVQEDSLDKTEFKALAMNHLKRLPGWDDQLYQTSEPAQDPDGMWHMDWRDPTPPKNLE
                     CGYKLVAKNSQLLRMMPYVKLDSQVQTAIAAEASGWKVAQRVAAIFIILFFVSGLAGM
                     IRTKNRSASSVIFMLVLLFNISFEGADTLTVKLRQIFGDAGVMGFVGGAVFVLGMLLI
                     YYSYPGLARKALPASKSRFTGYIPYLILIAIMAVQALGQDDLTSMQRYAYANGSRWIL
                     MNWLGAYLGFPVLCLGTALYYRHLSSRLASVRHGGWLSILLAVVLSASCLLIVNPQNY
                     SAPLVINSLIYLIVMTAGLYFIRYEADAKSHLIYSFSMLSLTLRIMPSAVGTTHEDIW
                     FRLLKIGLFIGMALLFDSFYRQSPWLWAKSRATGEKDRTP"
     gene            complement(2029..4095)
                     /locus_tag="PLO35_08185"
     CDS             complement(2029..4095)
                     /locus_tag="PLO35_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH79689.1"
                     /translation="MKILIVDDQFDNLYMLGSLLEGHGHSVIKAANGEEALTLLRRET
                     VDLVISDVLMPGMDGFTLCREIRKDPSLAGIPFITYTATYTGQKDEDFAREIGSDDFI
                     VKPCEPEELIARIDALMTRRQSGQPAAQPSSLEESEVLQLYNERLVRKLEKKMLETER
                     EVLARNEALEALRRSEEILNTTQKITRIGGWQYDLKSGQTYWTRELYHLHDLDPDIGG
                     MDPQQLASFSISCYAPHCRQLVTQAFELCKVTGQPYDMECAFTSAQGREMIVSLTGKA
                     IMEEGKVVKVYGAVQDITSLKEAEREQARLRTQLMQAQKLESIGRLAGGVAHDFNNIL
                     TVINGYSEEVLKSLVPGSQEYEDTEQILKAGHKATALVKQLLTFSRRQPGKTQPTNLN
                     RCLEDIISMLTRLLGETVNIRLDLEPKLDQVMADTGQLEQILINLAVNAKDAMPQGGT
                     LKFQTRNLDSTDPIYASYPDVPRNSYVILRVSDTGIGMNSFTMEHLFEPFFSTKDEKN
                     GTGLGLATVYGIVNQYQGYIRVESEPGEGVCFTILLPALQSEASSGHKDPASVAPSGD
                     QELIMVVEDDHSIRDMTSRLIRKLGFEVISAGSAMEAIRLYTEDNLKPAVLITDYIMA
                     GMNGLELAEQLRQINPELRIILMSGYIEKLSPTLDRNFQDIPFMQKPFTQAELAEKIK
                     QALGKP"
     gene            complement(4092..4484)
                     /locus_tag="PLO35_08190"
     CDS             complement(4092..4484)
                     /locus_tag="PLO35_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH79690.1"
                     /translation="MPNTILIIEDNPENFYLMKYLLENRGYRIFGAEAGKEGLKMAAE
                     LRPDCILLDIQLPGMDGYQVAVALRQQSCLDQVPVIAVTSYAMPGDREKAMASGASAY
                     IEKPIDPDSFVEEVECHIRAFQTQETAL"
     gene            complement(4484..6133)
                     /locus_tag="PLO35_08195"
                     /pseudo
     CDS             complement(4484..6133)
                     /locus_tag="PLO35_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1332 a         1734 c         1861 g         1625 t
ORIGIN      
        1 taggaggcca tgccacaggc gctgatcgcg ccttagcaaa cgatgacgtt tgctgctcca
       61 tgagtgggtg ctgttccgtt gttcccgcat cctatcacgg gtgcattacg ggtcggaccc
      121 gtaatgggat gcggggaaac agcagaatac cggtaatgca aaacagctcc atcggctata
      181 gttgcaaaca gaaataaata ttgacagcat cgccagtatc tcggcaatgg tatccacagc
      241 gtcgatgggt gagtggttcg ggatcaatgg ttccgggcca gcctggaaag ttttcaagac
      301 accccggcga aacaacaatt ccgcataggc aggtatgatt atatggccca caccgatgaa
      361 gttcagaacg ctgacaggcc cgcaacccgt gaaccgtcat ttatttatgt gtttatattc
      421 ctcctgctgc tgggcagctt caagctgaga gactccgcgc ttccggaaag cagcctgccc
      481 cgcatgaata tccttgaatc caaagcccag acagccgcca aaatctttct ggatagtgtt
      541 tggggacctg atgccacccg gggatggaat gcccgcctga tgtatcgcgc cttccaaaaa
      601 gtagagggag acgtgacccc cgctcccgag ctctatccca agcaggagat cgactatttg
      661 ctgcgtcacg cacccgctga gtatgtcagg ctggcagaac gttacaagct gggcgctctg
      721 gggctggtga ggttctctcc cgtttccaga gatcgcaaag aggctgtgga tgtatatctg
      781 agcggcagtt acgaagttta tgggatgatc ttgcacaact ggccagtgca ggaagacagc
      841 ctggataaaa cagagttcaa agctctggca atgaatcatt taaagcgttt accaggctgg
      901 gatgaccagc tctaccagac ctcggaacct gctcaggatc ctgatggcat gtggcacatg
      961 gactggcgcg atcccactcc ccccaaaaat ctggaatgcg gttataaact ggtagccaag
     1021 aactctcagc ttttgaggat gatgccatat gtgaagctgg actcccaggt tcaaactgcc
     1081 attgccgcgg aggcttcagg ctggaaagtg gcccaacggg tggcggcgat ctttatcatc
     1141 ctgttctttg tcagtgggtt ggcgggtatg atcagaacca aaaacagaag cgcatcatcg
     1201 gtcatcttca tgctggtctt gctgttcaac atatctttcg agggcgcgga taccctcaca
     1261 gtgaagctga ggcagatttt tggcgacgcg ggagttatgg gttttgtggg cggggccgtg
     1321 tttgtgctgg gaatgctgct gatctattac tcatatcccg gactggccag aaaagcgctt
     1381 ccagccagca aatcccgctt tactggctat ataccttatc tgatcttgat cgccatcatg
     1441 gccgtgcaag ccctgggcca agacgatctg acctccatgc agcgatacgc ctatgccaat
     1501 ggcagccgct ggattttgat gaactggctg ggagcgtatt tgggctttcc tgtattgtgt
     1561 ctgggaactg cactttacta tcgccacctg agctccaggc tggcctctgt cagacatggc
     1621 ggatggctct cgattctgct ggccgttgtt ttatcagcct cgtgtctgct gatcgtcaat
     1681 ccccaaaact attccgcccc tctggtgatc aattcgctga tctatctgat cgtcatgacg
     1741 gcgggcttgt atttcattcg ttacgaagcg gacgccaaaa gccacctgat ctatagcttc
     1801 tcgatgcttt cactgacgct gaggatcatg ccctcggcgg tgggtaccac ccatgaagat
     1861 atctggttcc ggctactgaa gataggcttg tttatcggta tggccctgct ctttgattcc
     1921 ttctatcgcc agtcgccttg gttgtgggcg aaatccagag cgaccgggga aaaggatagg
     1981 accccgtaag gactgcgggc taagcgctcc ggccagatac aagcctagtt atggcttgcc
     2041 cagcgcttgt ttgattttct cagccagctc ggcctgagtg aagggttttt gcataaaggg
     2101 gatgtcttgg aaattgcggt ccagggtggg agatagtttt tctatataac cggacatcag
     2161 gatgatccgg agctcggggt tgatttgtct gagttgttcg gccagttcca gaccgttcat
     2221 gccggccatg atgtagtccg tgatcaacac ggcgggtttt agattatcct cggtatatag
     2281 acggatggct tccatggcac tgcccgcgct aatcacctca aagcccagtt tcctgatcag
     2341 ccgggaggtc atatcgcgga tggagtggtc gtcttccacc accatgatga gctcttgatc
     2401 accgctgggg gcaactgagg ccgggtcttt atggcctgag gaagcttccg actgcagggc
     2461 tggcaggagg attgtgaaac acacgccctc gccgggttcg ctttccaccc ggatgtagcc
     2521 ctggtattga tttacgatgc cgtagacggt ggcgaggccc agtccggtgc cgtttttttc
     2581 atctttggtg gagaagaagg gctcgaagag atgctccata gtaaagctgt tcatgccgat
     2641 gcccgtatct gacactctca gaatcacata gctgtttctg ggaacatccg gataactggc
     2701 gtagatcgga tcggtggagt cgaggttgcg ggtttggaat ttgagggtgc cgccctgggg
     2761 catggcgtct ttggcattga cagccaggtt gatgaggatc tgctccagct gtcccgtatc
     2821 ggccatcacc tgatccagct ttggttccag gtccagacgg atgttcaccg tttcacccag
     2881 cagacgggtg agcatgctga tgatatcctc cagacagcgg ttgaggttgg tgggctgggt
     2941 cttgccaggc tgtctgcggc tgaaggtgag cagttgtttc acgagggcgg tggctttgtg
     3001 tccggctttg aggatctgct ccgtgtcctc atattcctgg ctgcccggca ccaggctttt
     3061 gaggacttcc tcgctgtaac cgttgatcac ggtgaggatg ttgttaaagt cgtgcgccac
     3121 tcctccggcc aggcgtccaa tggattccag tttctgggcc tgcatcagct gggtgcggag
     3181 ccgggcctgt tctctttcag cctctttgag cgaggtgata tcctgcaccg cgccatagac
     3241 cttgaccacc ttgccctcct ccatgatagc cttgccggtg aggctgacaa tcatctcgcg
     3301 gccttgggct gaagtgaagg cgcattccat atcgtagggc tggccggtga ctttgcagag
     3361 ctcaaacgct tgcgtcacca gttgtctgca atggggggca taacaggaaa tgctgaacga
     3421 ggcgagctgc tgggggtcca ttccaccgat gtcgggatcc agatcatgga ggtgataaag
     3481 ctcccgggtc caataggtct ggccggattt gaggtcatat tgccagccgc cgatccgggt
     3541 gatcttctga gtggtgttga ggatttcctc actgcggcgc agggcttcca gggcttcatt
     3601 cctggccagg acctcccgct cagtttccag cattttcttt tccagtttgc gcaccaggcg
     3661 ctcgttgtaa agctggagca cttcgctttc ttccaggctg ctgggttggg ctgcaggctg
     3721 gccgctttga cggcgtgtca tcagcgcgtc gattctggcg atcagctctt ccggttcgca
     3781 gggtttcacg atgaagtcgt ccgaaccgat ttcacgggca aaatcctcat ctttttggcc
     3841 ggtataggtg gcggtgtagg tgatgaaagg gatcccggcc agggagggat ctttcctgat
     3901 ctcgcggcag agcgtgaagc cgtccatgcc aggcatcaga acatcgctga tcacgaggtc
     3961 caccgtttcg cggcgcaaga gcgtgagagc ttcctcaccg tttgcggctt tgatcacgct
     4021 gtgcccatgg ccttccaaca ggctgccgag catatagagg ttgtcaaatt gatcatctac
     4081 gatcagtatc ttcataaggc ggtctcctgt gtctgaaaag ctctgatatg gcactccacc
     4141 tcttccacaa agctatccgg atcgatgggt ttttcgatat aggcactagc cccagaggcc
     4201 atggcttttt cacgatcgcc cggcatggca taagaggtga cggcgatgac ggggacctga
     4261 tcgaggcagc tttgttggcg caaggccacg gccacctgat agccatccat cccaggcagc
     4321 tggatatcca gcaaaataca gtccggccgc agttcggccg ccattttcaa gccttcttta
     4381 ccggcttccg ctccaaagat ccgatagccg cggttttcca gcaggtattt catcagataa
     4441 aagttttcgg ggttgtcttc aatgatcagg atagtgtttg gcattacttt ttctccaatc
     4501 gggccaggat ttccagggtg aaagtgctgc cctggccggg ttcgctgacc acccagatgg
     4561 tgcctcccag cagttgggca agttttctgc aaatagagag gcccagcccg gtgccttcat
     4621 aacgacgggt gagcccggaa tctatctgtc agaagggctg gaagagctgc agcaggtcct
     4681 ctttggcgat cccgattccg ctgtcccgga cggcaacgcg aagcttgtca ccctgcaggc
     4741 cgcaacggat ggatatttct ccccgctcgg tgaatttgac ggcattgctc accaggttga
     4801 tgatgatctg ttccagccgg cgctggtctg tgaatacgtc tatttggggt ggattgagtt
     4861 ccgtggtgag tttcaggttt ttggcctggg cggcgctgag catgatgtcg tagatcttgt
     4921 ggatgctgtc tgccagtgca aagccggagg gctcgaggtc cagctggccg gcctcaatct
     4981 ttgagaggtc cagcacttca ttgatcaggg acagcaggtg gcgggagctc ttttggatga
     5041 tggaaagctg cttgcgttgc tcgtcgttga tctttcccgg cagctcttgc aacaggatgc
     5101 ccgtgaagcc aatgatggaa ttgagcgggg tgcgcaattc gtgagacatg gtggccagaa
     5161 aggcggattt gagcaggtcc gattcctgag ctttgcgcat ggcgcgttcc agctcggcgg
     5221 tcctcagcat taccttggtt tccatctgat ggttgctttc ggaaagctcg gcggtgcgca
     5281 gcctcacctg acgcctcaga ataatgctgg cgatcagggt gaaagccaat cccagcatca
     5341 ataccagggc cagcactttg gtccaggcgg gaaacacata ctgggcccgg actggcaacc
     5401 agccacggat ggccctgtag taaggtgagt tctgatctcg tttgagctct gccaggcgtc
     5461 tgtcgatggc atccaggacg tcctgattgg tccccggacg ggtgccaaaa aagagattgg
     5521 acggttcaaa cacgatggaa gtgatctcca ttccatagct ggcggccttc agagtgccat
     5581 aaaacatgtt gctggcggcc aggtccgccc ggccttcctt cacggcccgg aaggcgttgt
     5641 cgaaactgct gaattttaga aagcttacgt ccagtccaaa gcctctgacg atgtctttta
     5701 gagcgctctc ctgaatggat ccctccaaca ccgcgattct aaggccatcc atgtccggga
     5761 tggatttcac gttgaggtcc ttgcgagcaa agatctggct ccaggaagcc agcatgggct
     5821 ctttgtgaaa ggcgaaaagc ctggcgcgtt ctggattgta ggccaggtcc ggcatcaggt
     5881 ccacctcttc gttttgcacc cgctccattc cctgttccca gcttccgggc acatatttca
     5941 gctgccatcc ttcccgccgc gcgatctcag cgaggatttc catgaggata cccgcgcccc
     6001 gggaatcttc acccagatag accttggggg gattgtcata gacggccacg ctcacggtcc
     6061 tgctccccag tccagtccac actgtggcaa ggagcggcag gcacaggacc agaagcggcc
     6121 atctaaagct catatgacat ccataactga gcgctttgag gcagagtccg gagttgaaat
     6181 ccacgctgga ttctccagat tgggatggca tgatgccaac ggcttttcat cttactcaaa
     6241 cctcgctttc ggagatgcgt cctctatagc ttaagcaggg acgccggatg aagcaaatac
     6301 attcttccgg cgggaaatat ctggttattt tcacagcaaa gcaagcttcc gatatcatca
     6361 actcatttgt ctggaatcct gccttgcctg gggaggatta tcccatccgg ttttagtgca
     6421 gatacttgac tcccacgcca ccggagtgtt tggtctcttt gagccagttc accttgccgt
     6481 cgccgtcctt gtctttaaag gtttcggcgt atttgccgat cacataccag cgtttgttga
     6541 tcctgaaggt ca
//
LOCUS       DAOSFN010000136         6450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_10494_length_6450_cov_9.529320, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000136 DAOSFN010000000
VERSION     DAOSFN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10494_length_6450_cov_9.529320"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PLO35_08200"
     CDS             complement(<1..764)
                     /locus_tag="PLO35_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79691.1"
                     /translation="MKRVAQFSLLALFICVSVLWGQSAVLVTDPATAVDFGVVVLEHH
                     FDRNISLMATGLAGTQLTIDNIQEFGDVTGSFEVISAPTLPFTMMAGTTETITVRYSP
                     QTADGHGGTLVISPVSSSPKNLNLQGTGRSLSLMSTPASYDFGCASTTGSANFWIGMD
                     PRYTMTSVVNVQTVTITGDPGFSIGGYWNSSWEPVTLPQSVMVGTDSLNILVNFDSGT
                     TSYLTAFLHVTDEYGDEIEVPLTAFRGLVPAPLNYD"
     gene            1016..3697
                     /locus_tag="PLO35_08205"
     CDS             1016..3697
                     /locus_tag="PLO35_08205"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOH79692.1"
                     /translation="MEINKAYEARQIEQKWYRWWEEKGYFKPAQSTDKPPFTILIPPP
                     NVTGILHIGHVLNNTLQDVAVRYQRMMGRPTLWLPGVDHAGIATQNMVEKNIAKDGLT
                     RHDLGREKLVEKIWEWKHEKGNIILDQLKLLGASCDWDRLRFTMDDMLSRAVKEVFVT
                     LYEQGLIYKGKYIINWCPRCVTALANDEVEHADEAGQLWHIRYPFTEGGGHVTVATTR
                     PETMLGDTAVAVNPKDERYKHLIGKTLDLPLTGRKIPVVADEYVDLEFGTGCVKVTPA
                     HDPNDFEIGRRHNLEQLLVMDEHGIMNSAAGADFEGMERYACRAKVLAMLSEQGLLAR
                     TEAHDHAVGHCYRCDTVIEPYLSDQWFVKMKPLAERAIAVVNSGEVRFQPERWTKVYM
                     HWMNNIRDWCISRQIWWGHRIPAFYCNHCNHMVVARELPAKCPNCASDSFRQDEDVLD
                     TWFSSWLWPFSTMGWPDDTADQQRFLPTSVLITAPEIIYLWVARMIMSTLHFKDKIPF
                     DTVLLHGTVRDELGRKMSKSLGNSPDPIDIIDKVGADALRFSMVFNTPKGADVIYSDN
                     ILETGRNFANKIWNAYRFIMMNMEEGQQLPSRAELKLELADRWIYSRLQQVIAQSADH
                     YENLRFNDAAQTIFQFLWDEFCSWYIELSKDRLMEPQTSPSGYNADNDPKSGSTARYI
                     LLDVMQTGMRLLHPVMPFITEEIWQGVKSIFPMPEPALITAAFPVSNPQLIDPAIADE
                     MAFMQESISAIRNLRKQVNLAPSQSIGIHVRVAEEPQAKPFTDYTAYLRKLAKVESAT
                     VGTAIQKPPASLAAVVRNIEIFLPLAGLVDLDAERARLAKQLEKISKEQAGVQAKLNN
                     PNFVNNAKAEVVEAERKRLDEVSVKLSLTKELLSDLG"
     gene            3757..3832
                     /locus_tag="PLO35_08210"
     tRNA            3757..3832
                     /locus_tag="PLO35_08210"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3790..3792,aa:Ala,seq:cgc)
     gene            complement(4038..5537)
                     /locus_tag="PLO35_08215"
     CDS             complement(4038..5537)
                     /locus_tag="PLO35_08215"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79693.1"
                     /translation="MKLKLMFIFLAASLVLGSLTAQCNGELTSVGDKYILQVWGSHYE
                     RGYAQGYLLAPRIMQVFSSYFFPYVAANSVGRYNNMLDYYVTYYETGAGFQSEAEGLV
                     AGMIASGQSLYHGPLQRDLNVGDMLLVNAIVDIPYATNLRDVDLACSSLSSYAEATLD
                     DPTLQGSLVITRLLDWTRNSNLINNPLLVVHHPSEPEEVKWMSFTYPGLIGALSAVNQ
                     YGHAAFLNMGNIHASSETYALDAVLLSVRKGIEIMNFNQDQLYNADDPWAAISGDQHI
                     SGTIIHNVWESSGEVMAQVIETNNSGTARRFYHENGNLNGRNLAATNHFRSLYPAVPC
                     YRYDNIQDALATDASMSVDRQWSLLGTAAGVSTNLMAIQYIPSSGVVKWSVATSTGPA
                     YSAPPYEFDADDLFAYEVATDDPTVPNLAFQLSVYPNPCPCEAELKFSSSNTLNRMGI
                     YNLRGQLLDSRPLSSAKGIISVAGLKAGIYFIRAEDTKGKAVVKKLLVF"
BASE COUNT         1535 a         1770 c         1714 g         1431 t
ORIGIN      
        1 aagtcatagt tcaggggagc aggtaccagg ccccggaaag cggtcagcgg tacctcgatt
       61 tcatcgccat actcgtctgt aacatgtaag aacgcggtta aatagctggt ggtgccacta
      121 tcgaaattga ccagaatatt cagggaatct gttccgacca tcacgctttg tggcagtgtg
      181 accggttccc agctggaatt ccagtatccg ccgatagaaa agcccggatc gccagtgata
      241 gtcacggtct ggacatttac caccgaggtc attgtatacc tggggtccat accgatccag
      301 aagtttgccg agccggtggt gctcgcgcag ccaaaatcat aactggccgg agtggacatc
      361 aggctcagag acctcccggt gccctgaaga ttgaggtttt tcggtgaact gctcacggga
      421 gagatcacca gagtcccgcc atgtccatct gctgtctgag gagagtatcg gacggtgatg
      481 gtctctgtgg tgccggccat catcgtgaaa ggcaaggtgg gagccgagat cacttcaaac
      541 gaaccggtga cgtctccaaa ctcctggatg ttgtcgatcg tgagctgggt gccggctaag
      601 cctgtggcca tcagagagat attgcggtca aaatggtgtt ccaggaccac tactccaaag
      661 tcaaccgccg tcgctggatc tgtgaccagg accgcgctct gcccccatag gaccgaaaca
      721 caaataaaca aagcgagcaa agaaaactga gcaactcttt tcatcttaac ctccctgttc
      781 ttctgaactt atggacgatg ataattgcct gagctggatt aatagattat tcactctata
      841 cgatggtcat atttgtccac tgacatggcc tgtcaaggta aatctttgct ggttcaagag
      901 agtggtcatg tctgcgccaa aacccgcttt tgcttgacac ccacggcccc ttcagaaaat
      961 ggcactttca caaaggattt tttactatag gcccaagcgg ccgcaaggaa gcagcatgga
     1021 gatcaacaaa gcatacgaag ccagacagat agaacagaag tggtatcgct ggtgggaaga
     1081 gaaaggctat ttcaagcctg cccaatccac cgacaagccc ccctttacca ttctgatccc
     1141 ccctcccaac gtgaccggca ttttgcatat cggccacgtg ctcaacaaca cccttcagga
     1201 tgtggcagtc cgctatcagc gcatgatggg acgccccacc ttgtggcttc cgggagtgga
     1261 ccatgccggt atcgccaccc agaacatggt ggaaaaaaac atcgccaaag acggcctcac
     1321 ccgtcatgac ctgggccggg aaaagctggt ggaaaagatc tgggaatgga agcatgaaaa
     1381 aggcaacatc atcctcgatc agctcaagct tttaggagct tcctgcgatt gggaccgcct
     1441 ccgtttcacg atggacgaca tgctgtcccg cgccgtcaaa gaggtgtttg tcaccctcta
     1501 tgagcaaggc ctcatctaca aaggcaaata catcatcaat tggtgtccgc gctgcgtgac
     1561 cgctctggcc aacgacgaag tggagcatgc cgacgaagct ggccagctct ggcatatccg
     1621 ctatcccttt acggagggag gtgggcatgt gaccgtggcg accacccgtc cggaaaccat
     1681 gctgggagac acggctgtgg ccgtcaatcc caaggacgag cgctataaac atctgatcgg
     1741 caagactctg gacctgcccc tcacgggcag aaaaatcccc gtggtggcgg atgaatatgt
     1801 ggacctcgag tttgggaccg gctgcgtgaa agtgactccc gcccatgatc ccaacgactt
     1861 tgagatcggc cgccgtcaca acctggaaca gctcctggtg atggacgaac atggcatcat
     1921 gaacagtgcc gcgggtgccg attttgaagg catggaacgc tatgcctgcc gcgccaaagt
     1981 gctcgccatg ctttccgaac aaggtctgct ggccaggacg gaagctcacg accacgccgt
     2041 gggccactgc taccgttgcg acaccgtgat cgagccctat ctttccgacc agtggtttgt
     2101 gaagatgaaa cccctcgccg agcgcgccat cgcagtggtc aacagcggtg aagtgcgctt
     2161 ccagccagag cgctggacca aggtctatat gcactggatg aacaacatcc gggattggtg
     2221 catcagccgt cagatctggt ggggccatcg catccccgct ttttactgca accactgcaa
     2281 ccatatggtg gtggccagag aactgcctgc caagtgtccc aactgcgctt ccgacagctt
     2341 ccgtcaggat gaggatgtgc tggacacctg gttcagcagc tggttgtggc ccttttccac
     2401 gatgggctgg ccagatgaca cggcagatca acagcgcttt ttgcccacca gcgttctgat
     2461 cacggccccg gagatcatct acctctgggt ggcccgcatg atcatgagta ccctgcattt
     2521 caaggacaaa attcccttcg acaccgtcct tctgcacggc acggtgagag acgaactggg
     2581 acgcaagatg agcaaatccc tgggcaactc acccgatccc atcgatatta tcgacaaggt
     2641 gggggccgat gccctgcgct tttccatggt cttcaatact cccaaaggcg cggacgtcat
     2701 ctacagcgac aacatcctcg agacgggacg caactttgcc aacaagatct ggaacgccta
     2761 cagatttatc atgatgaaca tggaggaggg acagcagctt ccgtccagag ctgagctcaa
     2821 actcgaactg gccgaccgct ggatctattc caggctccag caggtgatcg ctcagtccgc
     2881 cgatcactat gaaaatctgc gcttcaacga tgccgcccag accatcttcc agttcctctg
     2941 ggatgaattc tgcagctggt atatcgagct cagcaaagac cggctcatgg aaccgcagac
     3001 gtccccgtct gggtataatg ccgataatga ccccaagtca ggatccacgg cccgctatat
     3061 cctcctcgat gtgatgcaga ccggcatgag gctcctgcat cccgtcatgc catttatcac
     3121 cgaagagatc tggcaaggcg tcaagagcat cttcccgatg ccggaaccgg ctttgatcac
     3181 agcggctttc ccggtttcca atccccagct cattgatccc gccattgccg atgagatggc
     3241 ctttatgcag gaaagcatct ccgccattcg caatctgcgc aaacaagtca acctggctcc
     3301 ctcgcaaagc atcggcattc acgttcgcgt ggcggaagag cctcaggcaa agccgtttac
     3361 agactacacg gcctacctgc gcaagctggc caaagtggaa tccgccaccg tgggcacagc
     3421 catccaaaaa cctcccgcct ccctggcagc cgtggtgaga aacatcgaaa tcttcctccc
     3481 gctggcagga ctggtggacc tcgatgccga acgcgcgcgc ctcgccaaac agctggaaaa
     3541 aatttccaaa gaacaggctg gagtgcaggc taagctcaac aatcccaact ttgtcaacaa
     3601 cgccaaagcg gaagtggtgg aggccgaacg caaacgtctc gatgaggtga gcgtaaaact
     3661 ctccttgaca aaagaactgc tttcagattt ggggtaaaca cctgcacagc ggttgtttcc
     3721 gcttgcacaa gcgtatatta ttatctctca gatagtgggg ctatagctca gctgggagag
     3781 cggttggttc gcaatcaaca ggtcaggggt tcgagccccc ttagctccac caattatcaa
     3841 agcggctttc gagccgcttt tttgcgtgat cgccaaggta tcctggcata ataggccctg
     3901 tatactttaa acggttaccg cgcagtgctg catactgggc ataatattat gctacaatat
     3961 cttaagaatc gcatcacaac aaccctaaag accattgcgc ctggccgtgg atgaactcac
     4021 ctggcgcagt ctctcaacta aaacaccagc agcttcttca ccaccgcttt acctttagta
     4081 tcctccgctc taatgaaata gatcccggct ttcagaccgg ccactgagat gatgcccttt
     4141 gctgatgata gcggtcggct atccagtaac tgccctctca ggttgtatat tcccatcctg
     4201 ttgagagtgt tgctgctgga gaatttaagc tcagcttcgc aagggcaggg attgggatac
     4261 acgctcagtt ggaaagccag gtttgggacg gtaggatcgt cggtggccac ttcataggca
     4321 aacaggtcat cggcgtcaaa ttcataggga ggagcggaat aggcaggtcc ggtggaggtg
     4381 gccacagacc attttaccac tccactgctg gggatatatt ggatcgccat cagattggta
     4441 ctcacaccgg ccgcggttcc caaaaggctc cactggcggt ccacactcat agaggcatcg
     4501 gtggccaggg catcctggat attgtcatag cgatagcagg gtacggctgg atagagagag
     4561 cggaagtgat tggtggcggc aaggttgcgg ccgttcaggt tgccgttttc gtgataaaaa
     4621 cgccgcgccg taccgctgtt gttggtctcg atcacctggg ccatgacttc tccagagctt
     4681 tcccagacgt tgtggatgat cgtgccggag atatgctggt caccgctgat ggcagcccag
     4741 gggtcatcgg cgttgtagag ttgatcttga ttgaagttca tgatctcgat gcccttacgc
     4801 acggatagca gcaccgcatc caaagcatag gtttcggacg aggcatgaat gttgcccata
     4861 ttcaaaaaag ccgcgtgccc atattgattt acggcagaaa gagccccaat caatccggga
     4921 taggtgaagc tcatccactt cacttcttca ggctcggagg gatgatggac caccagcagg
     4981 ggattgttga tcaggttgct gttacgcgtc cagtccagca gacgagtgat caccaggctg
     5041 ccctgcagag tcggatcgtc caaagtggcc tccgcatagc tggacagcga ggaacaggcc
     5101 aggtccacat cccgcagatt ggtggcgtaa gggatatcca caatggcgtt taccagcagc
     5161 atgtctccca cgttcaggtc tcgctgcagg ggcccatgat acaagctttg tccgctggca
     5221 atcatacctg ccaccagtcc ttccgcttca gactgaaagc ccgccccggt ctcgtagtaa
     5281 gtgacgtaat aatccagcat attgttgtaa cggcccacgc tgttggcggc cacgtaggga
     5341 aagaaataac tgctgaacac ctgcatgatc ctgggggcca gcagatagcc ctgagcgtag
     5401 ccccgctcgt aatgagagcc ccacacctgc aggatgtatt tgtcacccac gctggtaagt
     5461 tcaccgttgc actgagcggt cagggagccc aggaccaggg atgccgcaag gaaaataaac
     5521 atcagtttta gcttcatatt cacctcgggg ctcagaattg gtaactgcgg atgcttgtca
     5581 agaactattg cgggcctggc agttgttgag ctttttgtct gtggacagag gcgtcagcgt
     5641 agttctccgg actgaaagga gtaaaagcgt tgatgtgtgc tccttattag cattacggtg
     5701 tcattacgga ctcattacgg actaagtccg taatgagtcc gtaataggat gcgggaagaa
     5761 ggcggcaagg atgggttatt atattgcaat gaatgtattt ggcgctctgc caatgagtgt
     5821 tggcactgtc cccaacccat tgtccctctt acaactctgg ctgagtccat tgtgcataac
     5881 cgtatcactg gtttcagact tgtgaaaaaa tgccaccgtg gagtttacat gataagcctt
     5941 gctgtcattc ccgtggagga gcttgtgcga aaatgcctta tagagtcccg tcaggggcga
     6001 tattttagat agacaacccg ctccctctct catgtatcta gccccttcag gggcggcatt
     6061 ttagatgcgt tgagcactgt tagacaacgg cttctgatgt gaggcaataa tctgaaatat
     6121 cgcccctgac ggggctcgga cctgtgtctg gtacgcgtaa tctagctaaa atagcgcccc
     6181 tgacggggct caatgcgcca ttttcactca ggctcggagt cgggaaacca gcccattgtg
     6241 taacaaaggg ttgtctgtaa gctggatttc cgtctccacg ggaatgacac tgaggtggct
     6301 ttggacttgc gtgcagtaca tcggaggcat ttatctcgcg ggtgtactgg ttgaagtcca
     6361 agcggcccgc aaactcgttg tcatgcatac ctttatcgtg gccgcttgaa ctccaacggg
     6421 accagtacct caagaatctc tgtccctccg
//
LOCUS       DAOSFN010000137         6386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_10672_length_6386_cov_9.916127, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000137 DAOSFN010000000
VERSION     DAOSFN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10672_length_6386_cov_9.916127"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1050..2021)
                     /locus_tag="PLO35_08220"
     CDS             complement(1050..2021)
                     /locus_tag="PLO35_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79694.1"
                     /translation="MIQDIFSTFGRFELGKLQHHGPLLIFPLLAKTPPLHKYISLRQA
                     LDNGELSISELSHAGSVPDLKVVNKGKHQVLILAGEELRGAKQNRVLNTTVLIAPDSE
                     LVVPVSCTEAGRWAYQDSHFSESGNMMAPSIKAKMTRSVNTSFMRGRSARSDQTEVWD
                     EIDKLQQNMQMPSPTAAMADVYNGSRHRLEDSLVRFPLLEGQCGIYVMLKGRFVGLDL
                     VSSPEVWRHLHAKIVKSYGMDALRFREPGYPADIHPPDLGELLSDLNFSVFPGIGLGE
                     ELRVEKPGITGSALVIDDEIIHLSLFPLEEEQMPIVERFHHPMRARG"
     gene            2412..3224
                     /locus_tag="PLO35_08225"
     CDS             2412..3224
                     /locus_tag="PLO35_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79695.1"
                     /translation="MYHGDGNDEAGYKTWLWGYGNRGLSNTTVLKRVWVYLQPGKAHK
                     YSRGYYNLICFASRNRMTQSELIRRLCRRADLDFWSSQISRSGCVFRRASHDLEAVRA
                     RLEGVPKAGRYGYFHRVLDHKLVRCNKEEVKYLHDPVRSEFQRRVQKEEEGGYRSLDE
                     LLHRGEIKREATQLYNWLLENWEPRSLNVLCYLYENMMSRPTIRLADESQDNVYQLKS
                     RLTRKLKDLIRERRVSEDVMRVLFEVFLQRLCQEIPASPTYRYRDEEDEPDL"
     gene            3208..4116
                     /locus_tag="PLO35_08230"
     CDS             3208..4116
                     /locus_tag="PLO35_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79696.1"
                     /translation="MNPIYEKQVFYALNELYLDSVRLDPDLDFDWDDLDCRRRLRRFR
                     ARDYSPESISDMEVPGSVRVMELGKSLICCLVLDSRSQGLWQALQLSMFTEFGATGDL
                     IVKARGGAYLALAGNSFLLTEEEISQSVVVDRVGLYDLMKAREFVEQPDRERYGRYLL
                     FGLPHEVRQSFVLSEFDLSCELRSRFLPEPGWLVLPAIIGEYYHARPMILFDMMSDED
                     IVLHQRSERIEPTMMKRPVSPIASIHGRDDVFEIVFDDHSVGQWASLYLKRLLIFRGK
                     LPASLKLKLPFSIDLMTLREKLKLSF"
     gene            4664..5620
                     /locus_tag="PLO35_08235"
     CDS             4664..5620
                     /locus_tag="PLO35_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79697.1"
                     /translation="MNSIESYIQILKQRGKTDSTLELASEVLGHYHEFVRDEVMSLES
                     VRSYCARHIAGKPDAADRIVVLFWYADHIKSKESGTYLVTLLGTLGVIESQRRRLIHL
                     YGEEAAVQVFSELTMPQLGSDLDAYPQAVTNYLQRMAEVLEPGSCQKVLAGNHHEVNP
                     EAFREDKELWQQSGDLDEFLKQKHQRLVARLQEHADTGKLWFEQYITQEVVDYVKEHQ
                     EIQTGIRQGDRIIVSKIPYNPDAWLKESDPLLKRFHACHCPFTRFSILTETPVPDLWC
                     YCSGGFTKLCFDYLYERDLEVELIESVLSGGESCRFAIKLPD"
     gene            complement(5699..>6386)
                     /locus_tag="PLO35_08240"
     CDS             complement(5699..>6386)
                     /locus_tag="PLO35_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79698.1"
                     /translation="YPGSTLSIANNKTISIWAGGIFYSFASASNPSLVTRNGASGNYV
                     FNLESGSMIAAEYTTFEYISIYGLNIKTGAMVDTYHAFTYCIFRNGPATSKLLTINNS
                     QTLTIDHANFPVLGSYNVSKTSDQGSLTFTNFTGDFSGATYEWDPYNRITWDMGIVPV
                     ITDLQIERQAPNNIRLWWTYPHPFTQFKIYACSTPGGTFELAGTTTNLYWVGSSSNAR
                     AFYKVVVVAP"
BASE COUNT         1578 a         1507 c         1809 g         1492 t
ORIGIN      
        1 tcacagcaga aactaaacat tccaaacctc agatgtcgtc ctgcacttcc aatggaaggt
       61 cgggaatggt gtatgcgctc aggagacgcc taccgggttc atttctgagt cgtattcgat
      121 ctgatcgttt tgatcccgga tagggagctt tgaactacaa ggggtgtcaa gctgccattt
      181 ccgcacaaac tctgaagcca gtgttacggc tatgtgcaaa ggtcttaatc gacaaggggt
      241 ggcacgaaag cacttgacgg cattgtggaa aagagaaagg ttgcgatatt gatgacccgg
      301 acgccgggct atcattacaa atgccccaat aatcggaaaa taagaagaag gcttgatgaa
      361 cataagactt acaatgacga ataccggatt tttgagcatt agaaggctgc actcacgcca
      421 ggggcatcag tttggtactg ggatatcagt gtgggagtat cctgaccctc cggaaagagc
      481 actttggctg tctcggtttg gtgagaattc ctggtttgga tcaaagtgcc tgaacaatgg
      541 atcaagatca tgaattggat agatttcatt acgggatttt gtcacttctg ttgcgccctt
      601 tttaagctcg cctggtttta gcctacccct cggatgtcca agtccagtcc aaaatcggcc
      661 tgaatttgaa gtaaaggtga tataaatacg cccaaatacg tccaaaacag tcgatgtcca
      721 agtccaaaca aagtccatca tatctactca ccacataaga ctttatccct gtttgtccaa
      781 gtgagggtca tttgaagttt ggggtgaggg ttaatactgg caccaggggt taccactcat
      841 ctcacaatct ccactcagga cctgactcca ggtctttaca gggtgttttt gtgcaccagc
      901 gatcggcaat acgcctatgg gaacctactg atcaacccat cagggagtcg ataagaagcc
      961 gggatcaggg tcattacgtg agatcggtga tagatgaaga gcggtgagct gtcccaccgc
     1021 tcttctcaag ccccttcaaa atctggggtt tatccgcggg ctctcatggg gtgatggaaa
     1081 cgctctacta tcggcatctg ctcttcctcc agaggaaaga ggctgaggtg gatgatctca
     1141 tcatcgatca cgagggccga gcctgtgatc ccgggctttt cgacacgcag ttcctctccc
     1201 agaccgatgc cgggaaaaac gctgaaattg aggtcgctta gcagctctcc aagatcgggc
     1261 gggtggatat ctgccggata tccgggctcc cgaaagcgca gcgcgtccat gccataggac
     1321 ttcacgatct tggcgtgcag atgcctccag acttcaggac tggagaccag gtccagcccc
     1381 acaaagcgtc ctttcaacat cacatatatc ccgcattgcc cttccaggag tggaaaccgg
     1441 accaggctgt cttccaggcg atgtctgctg ccgttataga catccgccat ggcggcggtg
     1501 ggagatggca tctgcatatt ctgctgcagt ttgtcgatct catcccacac ctctgtttgg
     1561 tcggaacggg ccgagcgacc acgcataaag gaggtgttga cgcttctggt cattttggct
     1621 ttgatgctgg gggccatcat gtttccggat tcgctgaaat gggagtcctg ataagcccag
     1681 cgtcccgctt ccgtacaaga aaccggcacc accagttcgc tgtccggagc gatgaggacg
     1741 gtggtgttga gtacgcggtt ttgttttgcg ccgcgcagtt cctccccagc cagaatcagg
     1801 acctggtgct tgcccttgtt caccaccttg aggtctggaa cgcttcccgc gtggctgagt
     1861 tctgagatgc tcagctctcc attgtcaagc gcttgacgca gagagatata cttatgaaga
     1921 ggcggcgtct tggccagtag gggaaagatc aacaggggac cgtgatgctg aagcttcccg
     1981 agctcaaagc ggccgaaagt gctgaaaatg tcttgaatca tgtgttcctc catggatact
     2041 gaagcatctc tacgctgctt ctactattct atatgcggga ggagtgcggg aacctgacaa
     2101 aaaacactta cgctgcaaca cagagaaaag cagaggtttt ctcaaaacag agggacggag
     2161 aattgacgat tgagaattga cgattgagaa ttgacaattg gggagagata tgagaatatg
     2221 agcattcctg tggagccgca aacgtcctcg tttgcgtggg ttcttagcgt cgaattccaa
     2281 ttcggcgcgt gcccataacg cataacccat aacgcataac gcgtaacgca taacgcatat
     2341 catctttggc cttgttaaac caggcttgcg gcttaaatta taaatgtaac gttttctatc
     2401 aaggagagat tatgtatcac ggggatggca acgatgaagc gggttacaag acctggctat
     2461 ggggatacgg caatcgtggc ctgagcaaca caacggtcct gaagcgggta tgggtgtatc
     2521 tgcagcccgg taaagcccac aaatacagcc gcgggtatta caacttgatc tgcttcgcga
     2581 gcaggaacag gatgacacag agtgaactga tccgcagact ttgccgcagg gctgatttgg
     2641 acttctggtc cagccagata tccagatcgg gttgtgtttt caggcgcgcc agccatgatc
     2701 tcgaggcggt ccgtgcccgt ctggaagggg ttccaaaagc gggcagatac gggtattttc
     2761 acagggtgct ggatcacaaa ttggtccgtt gcaacaaaga agaggtcaaa tatctgcacg
     2821 atccggtgag atcggaattt caaaggcgcg tccaaaaaga ggaggaaggc ggatatcgca
     2881 gcctggatga gctgttgcac cgaggcgaga tcaaacgcga ggcgactcag ctctataact
     2941 ggctcctgga gaactgggag ccccgcagcc tcaacgtcct ttgttatctc tatgaaaaca
     3001 tgatgagcag gcccaccatc agactggcgg acgagagcca ggacaacgtc tatcagctca
     3061 aatccaggct gacgcgcaag ttgaaagacc tgatcaggga aagacgtgta tcggaagatg
     3121 tgatgagggt gctgtttgag gtcttcctgc aaagattatg tcaggaaatt cccgcctccc
     3181 ccacatatag atatagggac gaagaagatg aacccgatct atgaaaagca ggttttttac
     3241 gcgcttaatg agctgtacct ggactcggtg cgcttagatc cggacctgga ttttgactgg
     3301 gatgacctgg actgcagacg tcggcttcgc aggttcagag ctcgggatta cagcccggag
     3361 agcatatccg acatggaagt tccgggcagc gtgagggtga tggaactggg gaaaagcctg
     3421 atctgctgcc tggtattgga ttccaggtct cagggactgt ggcaggcgct gcagctcagt
     3481 atgttcaccg agtttggggc cactggagat ctgatcgtca aagcgcgggg aggcgcctat
     3541 ctggctctgg cgggaaacag ctttttgctc actgaggaag agatcagcca aagcgtggtg
     3601 gtggacaggg tgggcttgta tgacctgatg aaggcccggg agtttgtgga gcagccggat
     3661 cgggagcggt atgggaggta tctgctgttt ggccttcccc atgaagtacg ccaatccttt
     3721 gtgctctcag agtttgatct cagctgcgag ctgcgttccc gcttcctgcc agagccaggc
     3781 tggctggtgt tgcctgccat cataggcgag tattatcatg ctcgaccgat gatcctgttt
     3841 gacatgatga gcgacgaaga catagtttta caccaaagat ccgagaggat tgagccgacg
     3901 atgatgaaga ggcccgtcag tcccattgca tccatccacg gtcgggatga tgtctttgag
     3961 atcgtttttg atgatcacag cgtgggccag tgggcatccc tgtatctgaa gagattgctg
     4021 attttcagag gaaagctgcc tgcttccctc aaactcaaat tgcccttctc catcgacctc
     4081 atgaccctga gagagaagtt gaaattgagt ttctaagccg gggctaagag tctccggtcc
     4141 ggacaccgcg attgcctttc atcaacggcg ctggtcagtt gccaggtatt cccgggacct
     4201 caagtggacg ggaaagatac tatctcataa ggagatatgt gcttatactc ggcctatgag
     4261 ttcaggttat cagaaaatag atcaagtctc caagcaaagc caggacggcc atgcccaaga
     4321 tggaccaacg catgcccttg ttggtatcac cagaggataa aaagaaaaaa accagtgcaa
     4381 ttgcggccat ggtaatgctc aaagcaggta tcatcagagt ggtaagcgag catattgccg
     4441 ctccaaaacc gacagcagtc ttccagtttt gcataagacc tcccttagtc atgatgaatt
     4501 acattatata ttaatcgcat atactgcctt gacaagagag gatgcggaaa catgaatcct
     4561 gagatcaggg aacagacctc tatccgcatg atttgaagaa agggcttgac cagatggggg
     4621 aggctcaaaa aattgaggac gattctgccc gagtggaggt ccgatgaaca gcatagaaag
     4681 ttatatccag atattaaagc agcgtggaaa gacagattcc accttggagt tggccagtga
     4741 ggtgctggga cattatcatg aatttgtccg ggatgaggtc atgagcctgg agtccgtgag
     4801 gtcatactgc gcgcggcata tagccggaaa accggatgcg gcagaccgga tcgtggtcct
     4861 cttctggtat gccgaccaca tcaagagcaa ggaatccggc acctatctcg tgactctgtt
     4921 gggaacgctg ggtgtaatcg agagccaaag acgcaggttg attcatctct atggcgaaga
     4981 ggccgccgtg caagtgttct ccgagctcac gatgccccag ttgggctctg atctggatgc
     5041 ttatccccag gccgtaacca actatctgca gagaatggca gaggttttgg agcccggctc
     5101 ctgccagaaa gtactggccg gaaaccatca cgaggtaaac ccggaagcct ttcgggagga
     5161 taaggagctc tggcagcaga gcggggacct ggacgagttt ctgaagcaaa aacaccagcg
     5221 cctggtggcc cggctgcaag aacacgcgga taccggtaag ctgtggtttg agcagtatat
     5281 cacccaggaa gtggtggact atgtgaagga acatcaggag atccagactg gcatccgtca
     5341 gggagacagg atcatcgtga gcaagatacc ctacaatccc gatgcctggc tcaaggaaag
     5401 cgacccgctt ctgaaacgtt ttcatgcctg tcattgtccc tttacccggt tttccatcct
     5461 caccgaaact cccgtgccgg acctttggtg ctattgcagc ggtggtttca ccaagctctg
     5521 cttcgattat ctgtatgagc gggacctgga ggtggaactg atcgaatcag tactctcggg
     5581 tggcgagagc tgccggttcg cgatcaaact gccagattag cttgtcttta taggagcata
     5641 tgaaatataa aaggcccctc cattggaggg gcctttagct tagtaccggg aaacctgtct
     5701 atggggctac cacgactact ttatagaaag cccgagcatt gctggagctt cccacccagt
     5761 agagatttgt ggtggtgcct gccaactcaa aggtgccgcc tggagtgcta caggcataga
     5821 tcttgaactg ggtaaaggga tgaggatagg tccaccagag ccggatgttg ttgggggcct
     5881 ggcgttcgat ttggaggtct gtgatcacgg gaacgatgcc catatcccag gtgatgcggt
     5941 tatagggatc ccattcatag gtggcgccgc tgaagtcacc ggtgaaattg gtgaaggtca
     6001 gggagccctg gtctgaggtt ttggacacat tgtagctgcc taagactggg aagttggcat
     6061 ggtcgatggt gagggtttgg gagttgttga tggtcagcag tttgctggta gccgggccgt
     6121 tgcggaaaat gcaatacgta aaggcgtgat aggtatccac catggcgccg gttttaatgt
     6181 taaggccata tatgctgatg tattcaaagg ttgtgtattc agcggcgatc attgagccgc
     6241 tttccaggtt gaagacatag tttccgctgg caccgttgcg ggttacaagc gacgggttgg
     6301 aagcgctggc aaatgagtag aagattcctc ccgcccaaat agaaatggtc ttgttgttgg
     6361 caatactcag agtggagccg gggtag
//
LOCUS       DAOSFN010000138         6303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_10877_length_6303_cov_8.844750, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000138 DAOSFN010000000
VERSION     DAOSFN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10877_length_6303_cov_8.844750"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLO35_08245"
     CDS             complement(<1..331)
                     /locus_tag="PLO35_08245"
                     /inference="COORDINATES: protein motif:HMM:NF026155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOH79699.1"
                     /translation="MRERILALYESRPDIYSAADRELFESFIEALNQGTVRSCEPTSK
                     GWIINTWVKKGILIGFRMGKLTPLPHTATKDFFDKDTLAERSFRIGDQVRIVPGGTSA
                     RNGCYIAP"
     gene            complement(331..1500)
                     /locus_tag="PLO35_08250"
     CDS             complement(331..1500)
                     /locus_tag="PLO35_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOH79700.1"
                     /translation="MAYDFDKIVDRRGSGCFKYDALKALYSRDDLLSLWVADMDFQVA
                     PCIQTAMKLRLEHGVFGYNLRMPDYYAAASYWVKKRYGWHVKRDWIVNTPGVVPAINL
                     AVLSLTRPDDKILIQTPVYRPFFNAVTDHDRCLLTNPLKLVNGKYQIDQEDFERKIRQ
                     ASLFILCNPHNPVGRVWDEYELQFMGDLCKRYDVPIVSDEIHADIIYEGKEFRGLGSL
                     ERYLDLTITCISPAKSFNIAGLSSAVAIIPNHRMRKSVNDLNEHLHLYMGNSFGIEAL
                     KAAYSEGEDWLEELLAYLESNRNYLMDYISTNLPMLKMIKPESTYLAWLDFGELGLDD
                     NALFDFLTNKARVALDPGRKYGVDGSGFSRLNFGCSKALLVEAMQRLTDAVKEGF"
     gene            complement(1504..2037)
                     /locus_tag="PLO35_08255"
     CDS             complement(1504..2037)
                     /locus_tag="PLO35_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79701.1"
                     /translation="MPQALSISSIKARMPVRYRFHPLIRWIMITFTALIAGYTIYFLV
                     RWVNADTPMFFKIVPLVILYIGLDALFRHLTALNEVIFTPECLWLRFIVKPAIPIPWE
                     NLRSMQLQKRITYYLYIVYTDLQGRQKLYKTPASFPKMTEIIFNIADLAPQVEMNQEL
                     AKMVAYLREISDKAETS"
     gene            complement(2022..2879)
                     /locus_tag="PLO35_08260"
     CDS             complement(2022..2879)
                     /locus_tag="PLO35_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOH79702.1"
                     /translation="MNTKYINPIKKINLHLHTSVSDGALAPRALIERSRAIGLDLISI
                     TDHDTTDAYRLIGEADLGLRLLPGIEISSQHEGNDVHILAYGYDIDAPGLNELTEMYL
                     IGRRDRAVKMLDKLSKLGMDIALDEVVAVAGSRELIVRPHIAQVMVAKGYCATKNEAF
                     EKFIGNFKPAYYPKPEVSVEDSLKVIHDAGAWAVIAHPGKLTDPSYLDLFIEMGIDGI
                     EVWHPDHSSSQVRDFNAKAMKNSLLMTAGSDFHGEQDRHNLMDIVPASAEVIDSVNFM
                     WSEYSCRKL"
     gene            2972..3760
                     /locus_tag="PLO35_08265"
     CDS             2972..3760
                     /locus_tag="PLO35_08265"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOH79703.1"
                     /translation="MRYEPLKDILASLLKCFPQARCLLFAALDLLLLRQSYVKAAIRN
                     YTPPETAPRMYDAGAGYCQYSWFMLRNWQHSKVFALDLKVDYIRAFSAWLDPVTRARF
                     SFTEGDLQFYQPKNSYDIITAIDILEHIPDDLAVLRNFYTCLKPGGKLIISTPSDTDE
                     AAKFTAEHVRPGYAMEDLRTKLESVGFEIIESRYSYGFWGSLAWKLSIRIPLNMIGRS
                     KVFMLVLPFYYLPILPLSLLFNKLDTQTFNPVGTGILMVAGKDQ"
     gene            complement(3958..4977)
                     /gene="tsaD"
                     /locus_tag="PLO35_08270"
     CDS             complement(3958..4977)
                     /gene="tsaD"
                     /locus_tag="PLO35_08270"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOH79704.1"
                     /translation="MTDPKLILAFESSCDDTSVAIVDTRYQVLCNLISSQPEHEAFGG
                     VLPELASRLHMRNIMRLTEAAFRKSGLSYSDIDAVAVSINPGLIGSLIVGLAFAKSFA
                     WALKIPLLTVNHMLAHVFSNFIQDPSIKAPFLALVVSGGHTELVHFEDQLSFKIVGKT
                     VDDAAGETFDKSAKIMGLGFPGGPIIDKLAKDGNPAFVTFPRGLDRKGNYNFSYSGTK
                     TAVMNHVRQLDPEELETHKADLAASIQQAIIEPLIKKSLAWARENAIADILLAGGVAA
                     NSALRDQLSAKADKYGIRVHYPGLKLCMDNAAMVAAAAIPKLLAGQYSGLDVNAFSLK
                     GTKLV"
     gene            complement(4974..5846)
                     /locus_tag="PLO35_08275"
     CDS             complement(4974..5846)
                     /locus_tag="PLO35_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79705.1"
                     /translation="MRKNLGLKALSLVLSLLVWLQIALSSEHHTIVRLPVVLDNVSPE
                     ITLSDLPKDVPFSVKGTGADLLKLRFSGVKVALNASELKPGSDKLPLENYSLRDFSVN
                     NSIEILGPALESDVSVTTDVLHHKSVKVVPAFENEAARTRYAEQDYLLTPDQIRINGP
                     RRVIRDLQSISTVPITLSMLGADRFDLPLSFSSSQISSTTPKVNVSRIPRRIEARVFE
                     ALRVEAGDNTVFPPIVTLKVEGTQEQVNALRPNSFVIKATQTPDADGWIELDVVLPEG
                     IDKYTLTPSRVRLR"
     gene            complement(5851..>6303)
                     /locus_tag="PLO35_08280"
     CDS             complement(5851..>6303)
                     /locus_tag="PLO35_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH79706.1"
                     /translation="PLRKRGDDILLLISYFFTNYASRFNISDKLDLNELRGILLSYDW
                     PGNVRELQNFCKSITIKEPEITNKVILQQLRNKISQQRNGGNTGMEKFLTIPSIREST
                     AAFEREYLIHYLDENAWQVSKTAAAIGIERTTLYKKMKQLGIVPIEES"
BASE COUNT         1505 a         1551 c         1659 g         1588 t
ORIGIN      
        1 ccggagcgat gtaacagccg ttgcgagcag aagttccgcc agggacaatc cgcacctggt
       61 cgccgattct gaagcttcgt tccgccaggg tgtctttgtc gaaaaagtct ttagtggcgg
      121 tgtggggaag gggagtgagc tttcccatcc ggaagccaat caggatgcct ttcttgaccc
      181 aggtgttgat tatccagccc ttggaagtgg gctcgcatga tctcaccgtg ccttgattaa
      241 gagcctcgat aaagctttcg aagagctctc tgtccgccgc ggagtagata tctggacggg
      301 attcatatag cgccaggatt cgctcgcgca tcagaagcct tccttaacag cgtcagttag
      361 ccgttgcata gcttccacca ggagcgcttt ggaacaaccg aaattaaggc gactaaagcc
      421 tgaaccgtca acaccgtact tacgtcctgg atccagtgct acccttgctt tgttggtgag
      481 aaagtcaaac agggcattgt catcgagtcc cagctcacca aagtccaacc aggccagata
      541 ggtgctttcc ggtttaatca ttttcagcat gggaagatta gtacttatat agtccatcag
      601 atagttacgg ttgctctcca ggtaagcgag caactcctcc agccagtcct cgccctcgct
      661 gtaagctgcc ttcaaagcct cgatgccaaa gctgttgccc atatagagat gcagatgttc
      721 attgaggtcg ttgacgcttt ttcgcattct gtgattgggg atgatcgcga ccgcggagct
      781 caaaccagct atattaaaag acttggcggg tgaaatacag gtgatggtga gatcgagata
      841 gcgttccagg cttcccaaac cgcggaattc cttgccctca taaatgatat cggcatgaat
      901 ctcatcggat acgatcggaa cgtcgtagcg tttgcacagg tcgcccataa actgcagttc
      961 atattcatcc cagacccgcc cgacgggatt gtgaggatta cagagaataa agaggctggc
     1021 ctggcgtatc ttacgctcaa aatcttcctg atcgatctga tacttgccat tcaccagctt
     1081 gagaggattg gtcagcaaac agcgatcgtg gtctgtaacg gcgttgaaga aagggcggta
     1141 aacgggcgtc tggatgagta tcttgtcgtc ggggcgagta agtgaaagca ccgcgaggtt
     1201 gatggcggga acgacgccag gagtgttgac gatccaatct cgtttcacat gccagccgta
     1261 acgctttttt acccaatatg atgcggcggc gtaatagtca ggcattctga ggttgtaacc
     1321 aaaaacgccg tgctccagtc tcagcttcat tgctgtctgg atgcagggag caacctgaaa
     1381 atccatatca gccacccaca gcgaaaggag atcatctctg ctgtaaagcg ctttaagggc
     1441 gtcgtacttg aagcatccgc tgcctcttct atccactatc ttatcaaaat catatgccat
     1501 agatcaactc gtttccgctt tgtcggaaat ctcccgcaga tatgctacca tcttcgcaag
     1561 ttcttgattc atttccactt gaggggccaa atcggcgatg ttaaagatga tttccgtcat
     1621 ttttggaaag gacgcggggg ttttgtagag tttctgccga ccttgaagat cggtataaac
     1681 gatataaaga taataggtta tgcgtttctg aagttgcatg gaacggagat tttcccaggg
     1741 gatggggatg gcaggtttga ctataaaacg cagccagaga cattccggtg tgaagatcac
     1801 ctcgttcagg gccgtcaagt gtctgaacag tgcatccagc ccgatataca ggatcaccaa
     1861 tggcacgatt ttaaagaaca taggcgtatc agcgtttacc catctgacca ggaaatagat
     1921 ggtatagcca gctatcagcg cggtaaaagt gatcatgatc cagcggatga gcggatggaa
     1981 gcgataccgc accggcattc tggctttgat ggacgagatg ctcagagctt gcggcaagag
     2041 tattcactcc acataaagtt aacactgtct atcacttccg cggaagcggg cacaatatcc
     2101 atcaggttat ggcggtcctg ctcgccgtga aaatcacttc cggcagtcat caaaagactg
     2161 tttttcatag ctttggcatt gaaatcacgg acctgagagc tgctgtggtc gggatgccat
     2221 acttcaatac catcgatgcc catttcaatg aacaagtcaa gataggaggg gtcggtaagc
     2281 ttgccggggt gtgcgatcac agcccaggcg ccagcgtcgt gaatgacctt gagagagtct
     2341 tccacactga cctccggctt gggataatag gcgggtttga aattgccgat aaacttttca
     2401 aaagcctcat ttttggtggc gcaatagccc ttggcaacca ttacttgcgc tatgtgaggc
     2461 ctcacgatca gttcgcggct gccagctacg gccactactt cgtccaaagc gatgtccatg
     2521 ccaagcttag ataacttatc cagcatcttt acggcccggt cccggcgtcc gatcaaatac
     2581 atctctgtca gctcgttcaa gccgggagcg tcgatatcgt aaccataagc cagtatgtgg
     2641 acgtcgttac cttcgtgctg agagctgatc tctatcccag gcagcagacg caagcccaaa
     2701 tcagcctctc cgataagacg atacgcgtct gtcgtgtcgt ggtctgtgat agaaatcaag
     2761 tccagaccga tggcgcgcga acgttcaatc agagcccggg gcgccagagc gccatccgaa
     2821 acgctggtgt gaagatgtag attgatcttt ttgatggggt ttatatactt cgtattcata
     2881 acattatttt cattgacgta aaactggcat aagcaaaatt cgtcaatgtt ttttttcacg
     2941 actgatccca ccagttccat aggagaatca aatgcgctac gaaccactca aagatatcct
     3001 tgcttccctg ttgaagtgct ttcctcaggc acgctgcctg ctctttgctg ctctggacct
     3061 gctgctgctg cgccaaagct atgttaaagc cgccatcagg aattacactc ctcctgaaac
     3121 tgctccccgc atgtacgatg ccggtgctgg ttattgccaa tatagctggt ttatgctgcg
     3181 taactggcaa cattccaaag tctttgccct tgatctcaag gtggattaca tccgtgcttt
     3241 ctctgcttgg ctagatcccg taacccgtgc gcgattctca tttactgaag gcgacctaca
     3301 attctatcaa cccaaaaaca gctacgatat aataaccgcc atcgacatcc tggaacatat
     3361 tcccgacgat ttggccgtgt tgaggaactt ctacacatgc cttaaacccg gagggaaact
     3421 gattatctcc acaccctcgg ataccgacga agcagcaaaa ttcactgccg agcacgtacg
     3481 tccaggctac gccatggagg atttgcgtac caaactggaa agcgtgggct ttgagatcat
     3541 cgagagcaga tacagctacg gattctgggg aagcctcgcc tggaagctca gtatccgcat
     3601 accgctcaat atgataggta ggtccaaggt tttcatgctg gtgctgcctt tttactacct
     3661 ccccattctc cccctttccc tattgtttaa caagcttgat actcaaacgt ttaaccccgt
     3721 gggaacggga atactgatgg tggcagggaa ggaccagtag agattctggg ttcttgagga
     3781 ttgaaggttc tgcgttgaaa agtcacttcc cattattctt gtggcgcatc aatcatagat
     3841 ttgagcatgc atcttgtggg tattgagaac tatgaaagga aaacgaaaaa gctatggctg
     3901 ataagtctgc aactccacaa tctctccact ccagctcagg gtgtaacgga aaggaactca
     3961 gaccagttta gtacctttaa ggctgaaggc gtttacgtct agcccggaat actggccagc
     4021 cagcaacttt ggaatggcag cggcggccac catggcagcg ttgtccatgc agagtttgag
     4081 accgggataa tgaacccgga tgccgtattt gtcagctttg gcgcttaact ggtccctgag
     4141 ggcggaatta gctgccactc cacccgccaa caggatgtcg gcgatcgcgt tttctctggc
     4201 ccaggcaagg gattttttga tgagaggttc tatgatggct tgctggatgg atgcggccag
     4261 atcggctttg tgggtttcca actcctcagg gtcgagttgg cggacgtggt tcataacagc
     4321 agtcttggtg ccgctgtagc tgaagttata gtttcctttg cggtccaggc ctctggggaa
     4381 ggtcacgaag gcaggattcc cgtctttggc cagtttatct atgatcggtc cgccggggaa
     4441 gcccagtccc ataatttttg cgctcttgtc gaaagtctcg ccagcggcat cgtccacggt
     4501 cttacccacg atcttgaaac tgagctgatc ctcaaaatgg accagttctg tatggccacc
     4561 agaaaccacc aaggccagaa agggcgcttt gatggagggg tcctgaatga aatttgaaaa
     4621 aacatgggcc agcatgtggt tgaccgtgag cagtgggatt ttgagagccc aggcgaagct
     4681 cttggcgaaa gcgagaccta cgattaatga gccaataaga cccggattga tggacaccgc
     4741 cacggcatca atatctgaat agctcagtcc actttttctg aaggcagcct cggtgagccg
     4801 cattatattg cgcatatgca gcctggaagc cagctcaggt aatacacctc cgaaggcttc
     4861 atgctcaggc tgggaggata tcagattaca cagcacctgg tagcgggtat ccacaatcgc
     4921 cacggaagtg tcgtcgcagg aagattcaaa ggccagaata agcttgggat ctgtcaacgc
     4981 aggcgaaccc tggaaggagt gagggtgtat ttgtctatac cctcaggcag cactacgtcc
     5041 agttcaatcc atccatcagc atcgggggtc tgggtggctt tgatcacaaa gctgttgggc
     5101 ctcagtgcat taacctgttc ttgagttcct tccaccttga gcgttacgat gggagggaag
     5161 acagtattat cgcccgcctc cactctcaag gcctcaaaca cgcgtgcctc tatgcgtctg
     5221 gggatcctgg aaacattaac cttgggagta gtggagctga tctggctgct gctgaatgac
     5281 agtggaaggt caaatctgtc cgctcccagc atggagagcg tgatcgggac ggtgctgatg
     5341 gactgaaggt cccggattac gcggcgggga ccgttaatcc tgatctgatc gggagtaagc
     5401 agataatcct gttctgcgta gcgggttcgg gcggcttcgt tttcgaaggc gggaactact
     5461 ttgacggatt tgtggtgcag tacgtcggtc gtaacgctga catcggattc cagggcgggt
     5521 cccaggattt caatggagtt gtttacactg aagtctcgga gactgtaatt ctccaggggc
     5581 agtttatctg agccgggctt tagttctgag gcattcagag ccactttcac tcccgaaaaa
     5641 cgcagtttca gcaagtctgc ccctgttcct ttcactgaaa aagggacgtc tttgggcaaa
     5701 tcggagagag tgatctctgg ggagacgttg tccaacacga cagggagcct cacgatggtg
     5761 tgatgttccg agctcagagc gatctggagc cacaccagca gagacaacac cagggataat
     5821 gctttgaggc ccaggttttt tctcacctga tcaggattcc tcgatgggga cgatgcccag
     5881 ctgtttcatc tttttgtaga gagtggtgcg ttcgatcccg atggcggcag cggttttgct
     5941 gacctgccag gcgttttcat ccagatagtg gatgagatat tctctttcga aggcggcggt
     6001 gctttccctg atgcttggaa tcgttagaaa tttttccatg ccggtgttgc caccgttgcg
     6061 ctgctgggat atcttattgc ggagctgctg gaggattact ttattggtga tctcaggctc
     6121 tttgatagtg atggatttgc aaaagttttg cagttccctc acgtttcctg gccagtcgta
     6181 tgaaagcaga atgccgcgca gctcgttgag gtccagcttg tcgctgatat tgaaccggga
     6241 ggcgtagttt gtgaagaagt aactgatcag cagcaagata tcgtctcccc gtttacgcag
     6301 agg
//
LOCUS       DAOSFN010000139         6164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_11210_length_6164_cov_9.168767, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000139 DAOSFN010000000
VERSION     DAOSFN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11210_length_6164_cov_9.168767"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /gene="panB"
                     /locus_tag="PLO35_08285"
     CDS             <1..638
                     /gene="panB"
                     /locus_tag="PLO35_08285"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOH79707.1"
                     /translation="DMLHHSAATRRGAPQSFIVTDMPFMSYHSIKAAKLNAADLIVQG
                     GANAVKLEGGSPSRVDAVKAIVDCEIPVCAHIGLTPQSVHRFGGYKVQGKTVKEHDEL
                     LRQAKALEAAGAFMLVLEGIPELLGKEITESIAIPTIGIGAGRHTDGQVLVWHDLLGF
                     SGMRPKFVKTYADLASSIQTALQDYATETREGVFPSQSQVYYPIKITEEHQ"
     gene            635..1264
                     /locus_tag="PLO35_08290"
     CDS             635..1264
                     /locus_tag="PLO35_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79708.1"
                     /translation="MKPKFEHTETMTPDEKFSAVANLSQALEDNFLTLGQLLSDIKRT
                     KLFKFKGYESFKDFIEAEYKMSGSLAGKLVQTFDLFIEEMDVDESTIKDIGFDRLQMI
                     RPLVQKADWGERDQWVEMAAEMPTKDLRDHIKEFKDKAKQEDTDLKKVYVDQYMEKML
                     AWFNCSRADLNFKLALYFQDADPESVKKVVKERQRAFETEVQDNKEDTP"
     gene            1270..2823
                     /locus_tag="PLO35_08295"
     CDS             1270..2823
                     /locus_tag="PLO35_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH79709.1"
                     /translation="MKILWVDDEIDLLKPFVLFLEERNYLVDTCNNGTDAIEQVLEHK
                     YDLVILDEMMPGLDGLTTLQEIKRINSSLPIVMVTKSEEEGLMDKAIASQISDYLIKP
                     INPNQIIMAIKKIFQADEIRANQIGEQYTRYMAQLNQRLFDAPDWKEWANIYREMAYW
                     ELQIDEVNDEGLRQTHFLEKRNCNTEFTNYVERNYRKWLMSDDRPNLSFDVVSQYIAP
                     NFEENVPIYFIIIDCMRLDQYLAIQPYIKELFDEELDLYYSILPTATPYSRNSIFSGL
                     LPIDIAKRFPEYWVTSGELDNSRNRNEHQLLDEHIKELGYSLEPASKYVKIFNMEEGN
                     FVLRKIETWNKENLVVLVYNFLDLLAHHRSRDQILQETIPHEEGLRAFTKHWFLHSSL
                     YEALRLIAKQDAICIISTDHGSIKVNRATQVVGDRDASITVRYKEGKNLTANDRHALF
                     AKKPSEYGLPSKSIVDNYIFAKDDYYFVYPNAYHQYQRQYNGTFQHGGVSLEEMILPI
                     ATCRPKRSK"
     assembly_gap    3015..3114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3115..3338
                     /locus_tag="PLO35_08300"
     CDS             <3115..3338
                     /locus_tag="PLO35_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79710.1"
                     /translation="WSSSGQDKAIYGSELAGRTRWSSSGQDKAIYGSELAGRLDFNQP
                     TEGTTRSDALLEVPRPSGRDLTVLSAQEL"
     gene            3438..3878
                     /gene="tsaE"
                     /locus_tag="PLO35_08305"
     CDS             3438..3878
                     /gene="tsaE"
                     /locus_tag="PLO35_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOH79711.1"
                     /translation="MNKIFKLNTPQDSAELATYLAPLLKCGDVLCLYGELGAGKTFFA
                     KEIGKALGIRDEIDSPSFVLLKEYHTGLLPLYHLDLYRLKTEDELMDLGIFDIIESGV
                     TLIEWPELAEKLLPYKTLSLRFVYDGQQRHVEISGEPRFTDHLD"
     gene            3903..4682
                     /locus_tag="PLO35_08310"
     CDS             3903..4682
                     /locus_tag="PLO35_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425332.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOH79712.1"
                     /translation="MFSNHLWLPIAVVLGLIALEIFEKDKGKHRGVIVASVAFVIGVT
                     VFGSFFLQSRQVDRGLKVTGYSSKLFESDLVKWTLSLQKGTSVDGLKDAYNSLSKDVA
                     DFKASLIAQGLTEKDINIQPPTSSPVNDTYGNLVSYSVNQTLYILSSDIPKVEKIALD
                     PSFFADRGLLMQQSNLEYLYTKLPELKKQLIAEATADALDRAKEITGSAKSKLGKLKS
                     ARAGVFQITEPYSTEVSDYGYYNTSTRSKSISVTVSTEFEL"
     gene            5146..5604
                     /locus_tag="PLO35_08315"
     CDS             5146..5604
                     /locus_tag="PLO35_08315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH79713.1"
                     /translation="MPKIINVREYGAGMETAVRYIHGIWGREENYAFYYDAIAHSSST
                     DMALPRFYLMLEAEEVIGCYALLTNDLVSRQDLWPWLGCLWIEPAQRGRELGKLLLQH
                     GVRVAGGLGYKTVYLTTDHDSYYERYGWIRMEDGYNLFGEKGRIYWHETR"
BASE COUNT         1590 a         1685 c         1446 g         1343 t
ORIGIN      
        1 cagatatgct tcaccatagt gctgccaccc gcagaggcgc tcctcaaagc tttattgtga
       61 cggatatgcc tttcatgagc tatcacagca taaaagccgc caagctcaac gccgcggacc
      121 tgatcgtcca aggtggcgcc aatgccgtca aactggaagg cggcagcccc tctcgcgtcg
      181 atgccgtcaa agctatagtg gattgcgaga tccccgtctg cgctcacatt ggcctcacgc
      241 ctcagagcgt gcatcgtttt ggtgggtata aggtgcaggg caagaccgtc aaagagcacg
      301 acgagctctt acgccaggcc aaagctctgg aagcagccgg cgcttttatg ctggtgctgg
      361 aaggcattcc ggaactgctg ggcaaagaga tcacggagtc cattgccatt cccacgattg
      421 gaatcggcgc gggccgtcac acagacggcc aggtactcgt ctggcacgac cttttgggct
      481 tcagcggaat gcgtcccaag tttgtaaaga cctatgccga tctggcatca tcaatccaga
      541 cagccctgca ggattatgcc acggaaacgc gtgagggcgt ctttccatct caatcacaag
      601 tatattatcc aataaagatc actgaggaac accaatgaaa cccaagtttg aacacaccga
      661 aaccatgacc cccgatgaaa agttcagcgc tgtggccaat ctgagccagg ccctggagga
      721 taacttcctc actctgggac agctgctctc agacatcaag cgtaccaaac tcttcaaatt
      781 taaaggctat gagagcttta aagacttcat cgaggccgag tataaaatga gcggcagcct
      841 ggccggcaag ctggttcaga cctttgatct ctttattgaa gagatggacg tggatgaatc
      901 caccatcaaa gacatcggct tcgatcggct gcagatgatc cgtcccctcg tccagaaggc
      961 cgattgggga gaaagggacc agtgggtgga aatggctgct gagatgccca ccaaagacct
     1021 cagagaccat atcaaggagt ttaaggataa ggccaaacaa gaagacacag acctcaaaaa
     1081 ggtgtatgta gatcagtata tggaaaagat gctggcgtgg ttcaattgtt cccgcgccga
     1141 tctcaatttc aagctggcgc tctactttca ggatgccgat cccgaatcgg taaagaaagt
     1201 cgtcaaagag cggcagcgtg ccttcgaaac cgaagttcaa gacaacaagg aggatacacc
     1261 ttgaagcaaa tgaaaatact ctgggtggac gacgaaatcg atctgctcaa accctttgta
     1321 ctctttctgg aagagcgcaa ctacctcgtg gatacatgca acaacggcac cgacgccatc
     1381 gagcaggtgc tggaacataa atacgacctc gtgatcctgg acgagatgat gccaggtctg
     1441 gatggcctca ccaccctgca ggaaatcaag cgcatcaaca gctccctgcc catcgtgatg
     1501 gtcaccaaga gtgaagagga aggcctgatg gacaaagcca tcgcctccca gatctcagac
     1561 tatctgatca agcccatcaa tcccaaccag atcatcatgg ccatcaaaaa aatcttccag
     1621 gcggacgaga tcagggcaaa ccagatcggc gagcaataca cccgctacat ggcccagctc
     1681 aatcagaggc tcttcgacgc tccggactgg aaagaatggg ccaacatcta tcgcgagatg
     1741 gcctattggg aactccagat cgacgaagtc aacgacgaag gcctgcgcca gacccacttt
     1801 ctggaaaagc gcaactgcaa caccgaattc accaactacg tggaacgcaa ctaccgcaaa
     1861 tggctgatga gcgacgaccg gcccaacctc tccttcgacg tcgtgtctca atacatcgcg
     1921 cccaatttcg aagagaacgt gcccatctat ttcatcatca tagactgcat gcgcctggac
     1981 cagtatctgg ccatccaacc ctatatcaaa gagctgtttg acgaggagct ggacctctat
     2041 tattctatcc tgcccaccgc cacaccctat tcccgaaact ccatctttag cggcctcctc
     2101 cccatcgaca tcgccaaacg ctttccggaa tactgggtca ccagcggaga actggacaac
     2161 agccgcaacc gcaacgagca ccagctgctg gacgagcaca tcaaagagct cggttacagc
     2221 ctcgagcccg cctccaagta tgtgaaaatc ttcaacatgg aagaaggcaa cttcgtgctg
     2281 cgcaagatcg agacctggaa caaggaaaac ctggtggtgc tggtctacaa cttcctggac
     2341 ctccttgccc atcaccgcag ccgcgatcag atccttcagg agaccattcc tcacgaagaa
     2401 ggtctgcgcg ccttcaccaa acactggttc ctgcattcct cgctctacga agccctcaga
     2461 ctcatcgcca aacaggatgc catctgcatc atctccaccg atcacggctc catcaaggtc
     2521 aaccgcgcca cccaagtggt gggagaccgc gacgcctcga tcaccgtccg ctacaaagaa
     2581 ggcaaaaacc tcacagccaa cgaccgtcac gccctctttg ccaaaaagcc ctccgagtat
     2641 ggcctcccct ccaagagcat cgtggacaac tatatctttg ccaaggacga ctactatttc
     2701 gtctatccca atgcctacca tcaatatcaa aggcaataca acggcacttt ccagcatggc
     2761 ggcgtctctc tggaagagat gatcctcccc atcgccacct gccgtcccaa acgttccaaa
     2821 taaacctggt ccgcgctttt ttgcttgacc acagccagaa agccggatag tatatactca
     2881 ggactgcaac cgaaacggtc ccaaaggcgg ctccgaatgc cggacctgct tttgggactt
     2941 tttattggtc accgattgtc ccgttggagt tcaagcggcc aggataaagc tatatatggc
     3001 agcgaacttg cgggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngttgga
     3121 gttcaagcgg ccaggataaa gctatatatg gcagcgaact tgcgggccgt acccgttgga
     3181 gttcaagcgg ccaggataaa gctatatatg gcagcgaact tgcgggccgc ttggacttca
     3241 accagcccac cgaaggaacc acgcgctccg atgcactgct cgaagtccca cgcccgtcag
     3301 gcagagattt aaccgtatta tctgcccagg agctttgaac tccaagttta catttactca
     3361 ctcccgtttt tcaagcttca tgtgagactg atatggcaat gccaaataaa tagattaaac
     3421 ataccaagga tatactcatg aacaagatat tcaagctcaa cacccctcag gacagcgcag
     3481 agctggccac ctacctggcc ccgctgctca aatgcggaga cgtgctctgc ctctatggcg
     3541 agctgggagc aggcaaaacc ttcttcgcca aagagatagg taaagccctc ggcatccgtg
     3601 acgaaatcga cagcccctcc tttgtgctgc tcaaagaata ccacaccggc ctcctgccac
     3661 tctatcatct ggacctctat cgcctcaaaa ccgaagacga gcttatggac ctcggaatct
     3721 tcgatatcat cgaaagtggc gtcaccctga tcgaatggcc ggaattggct gaaaaactgc
     3781 taccttataa aaccctctct ctccgttttg tctatgacgg ccagcagcgc catgtggaga
     3841 tctcgggaga gccccgcttt actgatcatc tggactaaac ctatttccga taaggagtta
     3901 atatgttctc aaaccacctc tggctgccca tcgctgtggt gctgggcctg atcgcgttgg
     3961 agatctttga aaaggacaag ggcaaacacc gcggcgttat cgttgcgtct gtagcctttg
     4021 tgatcggcgt caccgtcttt ggttcctttt tcctgcaaag ccgccaggtg gaccggggcc
     4081 tgaaggtgac gggttattcc agcaaactct tcgaatccga cctggtgaag tggaccctct
     4141 ctctccaaaa agggaccagc gttgacggtc tcaaagacgc ctacaacagc ctctccaaag
     4201 acgtggccga tttcaaggcc agcctcatcg cgcagggcct caccgaaaag gacatcaaca
     4261 tccagccccc caccagctcg ccggtgaacg atacttatgg caatctggta tcctacagcg
     4321 tcaatcagac cctctacatc ctctcttccg atatccccaa ggtggaaaag atcgccctcg
     4381 atccttcctt cttcgcggac cgcggactgc tgatgcagca aagcaacctg gaatacctct
     4441 acaccaagct gccggaactg aagaaacagc tgatcgcgga agcgaccgcg gacgctctcg
     4501 accgggccaa agagatcaca ggatcggcta aaagcaagct gggcaagctc aaatccgccc
     4561 gggccggggt cttccagatc acggagccct attccactga ggtttcggac tatggctact
     4621 acaacaccag cacccgcagc aagagcattt cggtaaccgt cagcacggag ttcgagctct
     4681 aaagctcccg ctctatcctg tctttcagac ctcgccgcaa gcggggtctg tttttatctg
     4741 ctgggtatcc caatctggaa taggtctgtt tattcgccaa acccgcttgc atcctcctgt
     4801 tggttccacc gctaaatcag gaaggtccaa ccaagaaagt ctaagctgga aagtccactg
     4861 gaaatccact ggaactacca tggaaataga taaaatcaca agataaaacc agtccctccc
     4921 ttggtccttc ccgcatccta ttacggactc attacggact tagtccgtaa tgagtccgta
     4981 atgataccgt aatgctatta aggaagagct tggataagct ggctccagac atgggccctg
     5041 ggcacttgta tattattgat atacaaacta ttagctatat tgattgacaa ggttgctaag
     5101 actggtataa ttgggtttgg aaaacaatcc tgccaggagt cagatatgcc aaagattatt
     5161 aatgtgcgtg agtatggggc cggcatggaa accgcggttc gttacatcca cggcatttgg
     5221 ggccgtgaag agaactatgc tttctattat gacgcgatcg cgcactcttc ttccacggat
     5281 atggccctgc cccgcttcta tctgatgctt gaggctgagg aggtgatcgg ttgctatgcg
     5341 ttgctcacca atgacctggt cagccgccag gatttgtggc cctggctggg ctgtctgtgg
     5401 atcgaaccag cccagcgtgg ccgcgaactg ggtaaactgc tattgcagca tggggtccgc
     5461 gtagcggggg gcttgggata caagactgtt tatctcacca ccgaccacga ctcttattat
     5521 gagcgctatg ggtggatccg gatggaggat ggctacaatc tctttggtga aaaaggacgg
     5581 atttactggc acgagaccag atagataaca tattcatcta taaagagata aacgcaccaa
     5641 aaaaaaagac cggaaaaaat gcttgacaac tattctgcct tcatttgaat gatacccatc
     5701 agaagtgatg cttctaacta atttttacag tgtttatgct tgcctgagat cgtggagcat
     5761 gttgtgagtg tttcatctca gacaggattg gccttatggc tgagcaggtt ttttttggag
     5821 aaagatgaaa caaatgtttt attatggtag ttttaattgt tgttttatca ctttatgtga
     5881 ggagcttggg tagcgtctgt gtttaatctg tcgtttcact caatcaatct aacccgttgt
     5941 gttaatggct caaagccagc actcttctta tccggccagt ttgtcgtaat catcgcacat
     6001 tatcacaaga ccgccggatc agacctggat ggcccagacc gtagttcatt tgtatcttca
     6061 aaatcaagaa aaagggccgg tcagctcttt ccgctgggag gtgcttagcc ggcccttttt
     6121 aacaacccga ttgaaacaaa ctacttagaa taatttctgg agaa
//
LOCUS       DAOSFN010000140         5994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_11664_length_5994_cov_10.290285, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000140 DAOSFN010000000
VERSION     DAOSFN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11664_length_5994_cov_10.290285"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1856
                     /locus_tag="PLO35_08320"
     CDS             156..1856
                     /locus_tag="PLO35_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79714.1"
                     /translation="MLINPKTPRAEIKMNLPKPKLVSAIAMCVFLILFILYFWIPVLN
                     PVFLDWIPAIAIAVLPLFFTTLLNKYIKYVYAFLALWVLIALLVSASIFRAKDYRNVI
                     GKVNQTEFSKLVSPVRLDQIPTVDSNYAAALAEKTLGEDFALGSRVTLGRPTRQMVDG
                     KLYWVIPLLHSGFFKWLANTAEGTPAYIKVSAINPRDVEFVRQVQGQPVRVKYQPNAY
                     FNQDLRRHIYLHGYMGYGIGGYTFELDDQGQPHWTITLFTHEVGVRAPNPVKLLTIHA
                     ETGKISEYPLIKTADGFSDEKVPAWVDRIQPAEFILTQLDWWGKYVRGFWNTLFSKRN
                     MLMVTAGYNIVYGTDDRSYVYSGMSSVGADEGTVGFVLSDTRTKRTHLYKMSGATEYA
                     AMRSAEGKVQNYKYQATFPILVNLNGLATYFMPLKDSAGLVKQFAFVSVKDYSVVGVG
                     ETIKAARDDYQMALSSSRVGNLAQKAAEEASLTGTVSRIGIDIKEGKTYYFLSFTEAP
                     GMVFIGSSDLSNYLPLTKAGDLLSVKYLKTEDKEISLISLRNDSLEPASQAAPAPGTE
                     "
     gene            complement(1990..3795)
                     /locus_tag="PLO35_08325"
     CDS             complement(1990..3795)
                     /locus_tag="PLO35_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79715.1"
                     /translation="MEAYKEQEFKGKFDLTLWISLIKQALPQRKRMAVLALVMIMNAL
                     VDTIFPLMTRYAIDKMIIPKNIAGISTFGFMYAGLVLFQFLMVYLLIDIAGRVETGMN
                     YDLRKNCYKKLQSMSFSYYDRTPTGWIVARLSSDISRLGDVVAWGIVDLVWGVAMLIF
                     TMGVVLSLSWQLALIMLALLPLITWIAIRFQISMFRSYRKVRKLNSMITSSFGEGIHG
                     AKTTKTLVRESANLDEFMTLNQDMYHHSVKAATASALFMPLILMVSSVGTGLILWRGG
                     ASVLAGAIGFGTLVAFINYTMQLFEPISNVARLFAELQNAQAAAERVFSLLELNPEIV
                     DSVPFTPDWQARRMQGHIRLEDMSFSYKENKPVFQSFDLEIAPGESIALVGETGTGKT
                     TLVNLICRFYEPTGGRILIDGMDYKELPLMWLHSQLGYVQQVPHLFQGSVMENIRYGR
                     LDATDAEVMEAARVVNADEFIQNLPDTYNYKVGESGNLLSTGQKQLIAFARVVLADPA
                     LLILDEATASIDTETEQLVQEAINKVLKGRTGIIVAHRLSTIRKVDRILLLHRGKILE
                     MGNHKELMKQGGRYHQLYLNQFMSEVTEHELRKEA"
     gene            complement(3808..5607)
                     /locus_tag="PLO35_08330"
     CDS             complement(3808..5607)
                     /locus_tag="PLO35_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79716.1"
                     /translation="MSSFRFVLQYMKGQRLPYLLAMLCVIGAAIMGIVNPLIIRYTVD
                     TVLGGKASAFPFGIGELMDQWNLAQITRSHLWLAALAVILVSLGAHSLTFLRGRLSAK
                     VSEQFARRLRDGLYDHIQRLPYAWHSKVQTGDIIQRCTSDVDTVRRFIGLQLVQLGRG
                     IFIVLLVLPVMISMSPRMTLISTAVVPVIFVYAYLFFKQVRKRFQEADESEGYLTTVL
                     EESLNGIRVVRAFAREDYEVKRFNGAADDYRHKCRKLITVLAWYWASSDALCLGQIAA
                     ITVFGIGWVIDGQISLGTLLAFSTYINMLLWPIREMGRILTDMGRAQVSVGRLREIME
                     TPVEEMDAPIRIGKGKRIKGEIEFKDLGFGYDPDHKILKGIDLHIRPGETIAILGATG
                     SGKSTLTNLIPRLFDGYEGQVLIDGKPLQSYHRQDLRRQIGIVLQEPFLYSRSLADNI
                     GIALPGVSRDRIEAVADEAALRESVLEFDQGFDTVIGERGITLSGGQRQRCAIARALI
                     HDPAILILDDALSAVDSETEQLIQRSLLERKGRSTTIIITHRLTGVSLADRIVVLEHG
                     RVVQTGPHHQLINEPGVYRRIWNIQHKLEREAS"
BASE COUNT         1291 a         1690 c         1668 g         1345 t
ORIGIN      
        1 cgcaaactcg ctgtcatgca tacctttatc gtggccgctt gaactccaac ggggcatacc
       61 gcagcggaca tccctttttg gccaaagcga ttctgcttgc cagaatttga ccgctcagga
      121 tcatgtcaca aaattcactc ccgaggagta taatgatgct aataaacccc aagaccccgc
      181 gcgccgagat caaaatgaac ctgccaaagc ccaaattggt cagcgccatc gccatgtgtg
      241 tgtttctcat cctctttatc ctctatttct ggataccggt gctcaatccc gtctttttgg
      301 attggattcc ggccatcgcg atcgcggtgc ttcctctctt ctttaccact ctgctaaaca
      361 agtatataaa gtatgtttac gcctttctgg ccttgtgggt gctaatcgcc ttgctagtct
      421 cggcatccat cttcagggcc aaggactatc gcaacgtgat tggaaaggtg aaccagacag
      481 agttttccaa gttggtttca cctgtccggc tggaccagat tcccacggtg gattccaatt
      541 acgccgcggc cttggcggaa aagactctgg gcgaagactt tgccctcggc agcagggtca
      601 ctctgggccg tccgacccgt cagatggtgg atggcaagct ctattgggtg attcctctcc
      661 tgcacagcgg tttctttaag tggctggcca acacggcaga gggaacccct gcctacatca
      721 aggtgtcagc catcaatccc cgcgacgtgg agtttgtgcg tcaggtgcaa ggccagccgg
      781 tgcgcgtcaa atatcagccc aacgcctatt tcaaccagga ccttcgccgt cacatctatc
      841 tgcatggtta catgggctat ggcatcggcg gctatacttt tgagctggac gaccagggcc
      901 agccccattg gacgatcacc ctcttcaccc acgaggtggg agtccgggcc cctaatcctg
      961 tcaagctgct caccattcat gccgaaaccg gcaagatatc tgaatatccg cttatcaaga
     1021 ccgcggacgg tttttcagac gagaaagttc cagcctgggt ggatcgcatc cagcccgcgg
     1081 agtttatcct cactcagttg gattggtggg gcaaatacgt gcgcggcttt tggaacaccc
     1141 tgttcagcaa gcgcaacatg ctgatggtga cggccggcta caacatcgtc tatggcaccg
     1201 acgaccgcag ctatgtctat tccggaatgt ccagtgtggg tgccgatgaa ggcacggtgg
     1261 gctttgtgct ttcggacacc cgcaccaaac gcacccatct ctataagatg agcggcgcca
     1321 cggaatatgc cgccatgcgt tccgccgaag gcaaggtgca aaactacaag tatcaggcca
     1381 ccttccctat cctcgtcaac ctcaatggcc tggccaccta ttttatgccg ctcaaagaca
     1441 gcgcgggact ggtgaaacaa ttcgccttcg tctccgtgaa ggactacagc gtggtgggcg
     1501 tgggtgaaac catcaaagcc gcccgggacg attatcagat ggccctgtcc agcagccggg
     1561 tgggaaacct ggcccaaaaa gccgctgaag aggccagcct caccggcacg gtcagcagga
     1621 tcggcattga tatcaaggaa gggaagacct actatttcct cagctttacc gaagctccgg
     1681 gaatggtctt catcggcagc tccgacctct caaactatct gccgctcacc aaagccgggg
     1741 atctgctcag cgtgaaatac ctcaaaaccg aagataaaga gatcagcctc atcagcctgc
     1801 gcaacgacag cctggagccc gcttcacagg ctgctccggc acccggcact gaatagcaga
     1861 ctcagatctc agccaaagca tatcgggacc acctcgcatt tatgggaggt ggtcccgatc
     1921 tggtatatgc cagggttcgc ctgcggaaca ggcaagctgc tagcgtgtca cagcgtaact
     1981 ctcacttcct cacgcttcct tcctgagttc atgctccgtc acctcgctca tgaactggtt
     2041 cagatagagt tggtgatagc ggccgccctg tttcatgagc tctttgtggt tgcccatttc
     2101 caggatcttg ccacgatgca gcagcaggat gcggtccacc ttgcggatgg tggaaaggcg
     2161 gtgggccacg atgatgccgg tgcggccttt gagcaccttg ttaatggctt cctgcacaag
     2221 ctgttccgtc tcggtatcga tggaggcggt ggcctcgtcc aggattagca gtgcaggatc
     2281 ggccagcacc actctggcga aggcgatgag ctgtttctgg ccggtggaga gcaggtttcc
     2341 gctctcgccc accttgtagt tgtaggtatc cggcaggttt tggataaact catcggcgtt
     2401 caccactctg gcggcttcca tcacttccgc gtccgtggcg tccagacgac cgtaacggat
     2461 gttttccatc acgctgccct gaaagagatg cggcacctgc tggacgtaac cgagctggga
     2521 atgcagccac atcaacggca gctctttata gtccatgccg tcgatcagga tgcggcctcc
     2581 ggtgggctcg tagaaacggc agatgaggtt gaccagggtg gttttgccgg ttcccgtctc
     2641 acccacgagt gcgatggact caccgggcgc gatctccaga tcgaagctct ggaacacggg
     2701 cttgttttcc ttataggaaa agctcatatc ttccaatctg atatgacctt gcatgcggcg
     2761 ggcctgccaa tccggagtga agggcacgct gtccacgatc tcaggattga gctccagcag
     2821 ggaaaagact cgttccgcgg cggcctgagc gttttggagc tcggcaaaga ggcgcgccac
     2881 gtttgagatg ggctcaaaga gctgcatcgt atagttgatg aaggccacca gggtgccgaa
     2941 accgatcgct ccggccagca cgctggcccc acctctccag aggatgagac cggtgcccac
     3001 gctgctcacc atcaggatca gcggcatgaa aagcgctgaa gccgtggcgg ctttgacgct
     3061 gtgatgatac atatcctggt tcagagtcat aaactcatcc agattggcac tttcgcgcac
     3121 cagagtcttg gtggtcttgg caccgtggat gccttcgcca aagctggagg tgatcatcga
     3181 attgagcttg cgcaccttgc ggtagctgcg gaacatgctg atctgaaagc ggatggcgat
     3241 ccaggtgatg agcggtagca aggccagcat gatgagggcc agctgccagg aaaggctcag
     3301 caccacgccc atcgtgaaga tcagcatcgc cactccccag acgagatcca caatgcccca
     3361 ggccaccaca tctcccagac gcgatatatc tgaagacagc ctggccacga tccatcccgt
     3421 gggggtgcgg tcgtaatagc tgaagctcat gctctgcagc tttttatagc agttcttgcg
     3481 gaggtcgtag ttcatcccgg tttccacccg gccggcaatg tcgatgagca gatagaccat
     3541 cagaaactgg aagagcacca gtccggcata catgaagccg aaagtgctga tcccggctat
     3601 gttttttggg atgatcatct tatcaatggc gtagcgggtc atgagcggaa agatggtgtc
     3661 caccagagcg ttcatgatca tcaccaaagc caatacggcc atgcgtttac gttgaggcag
     3721 ggcctgtttg atcaagctga tccacagggt caggtcaaac ttgcctttga attcctgttc
     3781 tttatatgct tccataataa tatcctgtta gcttgcttca cgttccagct tgtgctggat
     3841 gttccagatg cggcgataga cgccgggttc gttgataagt tgatggtgtg ggccggtctg
     3901 gactaccctg ccatgctcca gcaccacgat ccggtccgcc agcgaaacac ctgtgagccg
     3961 gtgcgtgatg ataatggtgg tgctgcgtcc tttgcgctcc aacaaactgc gctggatgag
     4021 ctgttccgtc tcggaatcca ccgcgctgag ggcatcgtcc aatatcagga tggcggggtc
     4081 gtggatcaaa gccctggcga tggcgcagcg ctgtctctga cctccggaga gggtgatgcc
     4141 gcgctcgccg atcacggtgt cgaagccctg gtcaaattcc aggaccgatt ctctgagagc
     4201 ggcctcatca gctaccgctt caattctatc tctgctcaca ccaggcaagg cgatgccgat
     4261 attgtcggcc agcgatctgc tgtagagaaa gggctcctgc aacacgatcc cgatctggcg
     4321 tctaaggtcc tggcggtgat agctttgcag aggcttgccg tcgatcagca cctgtccctc
     4381 atagccgtca aagagacgcg ggatcaggtt ggtgagggtg gattttccgc ttcccgtggc
     4441 acccaggatg gcgatcgtct ccccgggccg gatgtgcagg tctatgcctt tcagtatctt
     4501 gtgatcggga tcatagccaa agccgaggtc cttgaactcg atctcgcctt tgatcctctt
     4561 tcccttcccg atcctgatcg gcgcatccat ctcttccaca ggcgtttcca tgatctctct
     4621 gaggcgtccc accgaaacct gggcacggcc catatcggtg aggatgcgtc ccatctcgcg
     4681 gatcggccag agcagcatat tgatgtaagt gctgaaagcc aggagggttc cgaggctgat
     4741 ctgcccatcg atcacccagc cgatgccaaa cactgtgatg gcagctatct ggcccaaaca
     4801 gagcgcgtct gaagaggccc aataccaggc caggacggtg atcagcttgc ggcatttgtg
     4861 gcgatagtcg tccgccgcgc cgttgaagcg cttaacctca taatcctctc tggcaaaggc
     4921 cctcaccact ctgatgccgt tgagcgattc ctccagcacg gtagtgagat agccttcgga
     4981 ttcatccgct tcctgaaagc gcttgcgcac ctgcttgaaa aagaggtagg catacacaaa
     5041 gatcaccggc accacggctg tggaaatcag cgtcatgcga gggctcatgg agatcatcac
     5101 aggcagcacc agcaacacga taaagatacc gcggcccagc tgcaccagtt gaagaccgat
     5161 aaaccggcgc accgtgtcca cgtcagaggt gcagcgctgg ataatgtctc ccgtctgcac
     5221 cttactgtgc caggcgtatg gcagcctctg gatgtgatcg tagaggccgt cgcgcaatct
     5281 gcgggcaaac tgctcggaca ctttggcgct cagccttccc ctcagaaagg tgaggctgtg
     5341 ggcacccagg ctcacgagga tcactgccag agcggccagc cagagatggg aacgcgtgat
     5401 ctgggccagg ttccactggt ccataagttc tccgatccca aagggaaagg cgctggcttt
     5461 gccgcccagc acggtatcca ccgtgtagcg gattatgagc ggattgacga tgcccatgat
     5521 ggcggcaccg atcacacaga gcatggccag caaatatggc aaacgctggc ctttcatata
     5581 ttgtaaaacg aaacgaaatg atgacatggt gttcttactc catggtttga aagctggggt
     5641 ggataatact gaccggaaac gagtccggtt ttgtcaattg gaaagtggca acgggtccgg
     5701 tgggtttctc tgtgaaggtt gtttcagcct ctgtaaaggc tttcttaaga gcctgaacgc
     5761 tgttctgaag cgctttttgg cgcactatgc cggaacgggt accagctgtt ctatagggaa
     5821 gatcagttgc ggacgacgat gccgcgagct taccgggatg ggtttgtccc gatttttgtc
     5881 tgggccgggg gttcagttaa ggctgaacgt aggcaagcag gggcatcgga ttcacactat
     5941 gcgctggggt ggcgaaggct gtaatatgct gtttcatgtg tctttagctc ctgt
//
LOCUS       DAOSFN010000141         5949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_11772_length_5949_cov_6.668137, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000141 DAOSFN010000000
VERSION     DAOSFN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11772_length_5949_cov_6.668137"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            411..>5949
                     /locus_tag="PLO35_08335"
     CDS             411..>5949
                     /locus_tag="PLO35_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH79717.1"
                     /translation="MKSSCILWIALIVLSMIPLSLASQVGPANSAFSISRSASGSLDL
                     HFELPAWKLEEVSKDGESLQRVSITDTPYLYIGEDETLPIFSTTLAIPYRGGASLSVL
                     SAASDSRAVTSMDFDTLLNSEKAAGRLSDPLYPASSVLISEPMILRDFRIVTINVYPF
                     QYDSQTRELKISQSVDLRVSFDNGPSVNEMEAPLSYSSAFESIYRGMILNYDSFRDRD
                     TSYSSPVLLVMNGNFANAEFENRLTLYMDWKRQMGFKVYSYAVPSGSTSNTVKNYIQT
                     AYDTWEDRPDYVVLIGDVSGNIAIPTDANYNDYNYTQLAGNDVLGDIYIGRISVSETA
                     QFLAYTSKLFFYERDLVLSSATWMNKMLLVGDTASSGISTIYTNRYIRDMSEAVNPSY
                     TYTEMYQNNPSYLTMNSNLAAGVEIFNFRGWMGMSNWNSESNINSINNFNRPYHAVMI
                     TCATGSFGDGLSGTETVIRAGTDSQIKGGITAIGMATTSTHTPLNNCLDMGIFHGLYR
                     HNMRDMGTALLYSKLYLKSVYLNSGPTQYDQSIFFSKICNLMGDPTAPVYKGLPGQLT
                     VSMPASIPSGSQSVEILVKNAALQPVPNAVVTLTNSAGLFVSGITNEQGLIAPQFPGS
                     TLGPITVTVRKDGFKASVGQITLSSGSGFVYASHFADDGYSGISQGNGDGFVNGGEII
                     ELWLSLRNTSSTASDVSLVASSSSPYVNILDYHLDFNIVPAGQTAESTNPLQFSVSAD
                     CPDAYSLIISLADSGGQNLCQFPLNIQIRNGKPEISSHTLIGAPGNLVYPGDQYPLQF
                     DILNSGTGALTGLSATLRSYVPFCSVQDSLGTFGDVPAGNPGSNASDTFTINISPQAL
                     TGMLIPLELRFQNSQGFNYILSYSLTVGSVSVTDPLGQDAYGYFIYGMEDTSYMLAPT
                     YTWIGIAPAEGGSGTLLALTDPGVSGDEGDQTNASSITTVNLPFSFKFYGTDYTRASI
                     SSNGFIAFGQTQNSDWRNWRLPGPGGPTPMLAAFWDDLCLNSGGVYTWYNASQHYYIV
                     EWYDLTHGKDLSSRETFQAILYDPVYYPTLSGDGQIKLQYKVFNNIDDSVQGYPYPYG
                     NFCSIGIKDQTATIGLEYAFNNSFAAAAAPLGNETALFISTAPTPQIAPSLTLGTIYV
                     ADASGNNNGLLDTGENSGLNIRIINHGLNAATGVSATLSSSDPYLTITQNYSSYGSVP
                     GSGSAMPLTPYQVQVSASVPDGHQAVLNLNINSAQGSWNREFHLTLNAPLFEFGTMSI
                     LDPSGNNNGVLDPGETVTLRIVLANNGGAESSSGTASVSSPTPGISIINGSTSFTAVG
                     SSSTTLLSYTVAASSSLSEGTLATLSFHAGAGNYSADENRYVEVGMPLLVSIGNGTSV
                     QSYPIDRYYNYSVHESIYLASEIGGSGLLKSIGFYKGSGTDVSAIQSVTIYMKHTTAT
                     SLSSGNYSTTGYTQVYNGDFPNTATSGWMEVNLNPMFTYSADQNLSILIVKNFQQYIN
                     NYPQWRYTTTTGYRARQERSDSAMPTSLSASASLPNLQIRMFPVQEVLYPPQNLTAQA
                     THQRIDLGWSAPVSGTPVSYKLYRNSSLLTTVTALSYTDLAVTNGTSYSYYVTALYSN
                     PTGESEPSNSVDVTPTTIEFVIIGTGTSVSTNGQNSPINISNNSTHGQLVYTAAELNA
                     AGVTGPVLISGLGFYVVTSPDLALPNFMVRMKHTTAVDGSSWHDANGFVTVYSNASYM
                     PSAGGWDMLMFSAPFEWNGEDNLVVDTAFGVVAQASQTGTMQYTTIANGYRFAWSNTT
                     NQSNIFTGGLAVNRRYNVRLILQDIPSGPSIEVEPATIPFGNVPVNTSSIQQFSIENT
                     GDTV"
BASE COUNT         1407 a         1801 c         1393 g         1348 t
ORIGIN      
        1 ttggagcgga gatgtgagag ctacgctggt gagagccgtt ccggctggtt agagcagttc
       61 ctgctggtta gagctctgcg agctggttag agcagttcct gctggttaga gcctttggct
      121 ggttagagca gttcctgctg gttagagcct ttggctggtt agagcactgc gtgctggtta
      181 gagctctgcg agctggttag cctgtgagaa aaagaccata cagtctcaca agcacaacgt
      241 gctctcacca gccggaacgg ctctcacatg cgcagcatct cacctgacgc agtcatctca
      301 catgcgcagc atctcaccta cgcagtatct ctcctaaaaa tatctcactt ttgcaccgct
      361 atttgcaaaa catcacagca gtgtaacaat ccacccccaa aggaaccccc atgaaatcaa
      421 gctgtatcct gtggatagcc ctcattgtgt tgagtatgat cccgctgtcc ctggcctccc
      481 aggttggtcc tgccaactcc gctttttcga tatcccgctc cgcatccggg agccttgacc
      541 tccattttga gctgccggcc tggaagctgg aagaggtctc caaagacggc gagtccctcc
      601 agcgggtcag catcaccgac accccctatc tctatatcgg cgaggatgag accctgccga
      661 tcttcagcac caccctggcc attccctatc ggggcggggc cagcctcagt gttttgagcg
      721 ccgcaagcga ctccagggcc gtcacttcca tggattttga cactctgctg aacagcgaaa
      781 aagccgccgg acgcctctcc gatcctctct acccggccag ctcggttctg atctcggagc
      841 ccatgatcct gagagacttc cggatcgtga ccatcaacgt ctatcccttc caatacgaca
      901 gccaaacccg cgagctaaag atcagccagt ctgtggacct cagggtcagc tttgacaacg
      961 gccccagcgt caacgagatg gaagctccgc tcagctattc cagcgctttc gagagcatct
     1021 accgggggat gatcctcaat tatgacagct tcagagaccg cgatacatcc tattccagcc
     1081 ccgtattgct ggtaatgaat ggcaactttg caaacgcgga gtttgaaaac cgtttgactc
     1141 tctatatgga ctggaaaaga cagatgggct tcaaggtcta tagctatgcc gttccgtccg
     1201 gctccacttc aaacactgtg aaaaactaca tccagacagc ctacgacact tgggaagaca
     1261 ggcccgatta tgtagtattg atcggggacg tttccggcaa catcgcaatc cctaccgacg
     1321 caaactataa cgactataac tacacccagc tggcaggtaa tgacgtgctg ggcgatatct
     1381 acatcggcag gatatctgtg agtgaaaccg ctcagtttct tgcctataca tccaagctat
     1441 tcttctacga gcgggatctg gtgttgagct ccgccacctg gatgaataag atgcttttgg
     1501 tgggagatac cgcatcctct ggaatttcca ccatctacac caatcgttac atccgggaca
     1561 tgagcgaggc ggtcaacccc tcctacactt ataccgagat gtatcaaaac aatcccagtt
     1621 acctgaccat gaacagcaat ctggccgccg gagtggagat ctttaacttt cgcggctgga
     1681 tgggaatgag caactggaac agtgaatcca acatcaacag tataaacaat tttaaccgtc
     1741 cttaccacgc tgtaatgatc acttgcgcta cggggtcttt tggagatggt ctcagcggca
     1801 ctgaaacagt gattcgggcc ggaaccgatt ctcagatcaa gggtggcatc acagccatag
     1861 gcatggccac cacttccacc catacaccat taaacaactg cctcgacatg ggtatttttc
     1921 acgggctgta tcgccataac atgagagaca tgggtactgc cctcctctat tccaagctct
     1981 atctgaaatc ggtgtatttg aacagcggcc ccactcagta tgatcaatcg atctttttct
     2041 ccaagatctg caacctcatg ggcgacccca cagccccggt ttataaagga cttcccggtc
     2101 agttgaccgt cagtatgccg gccagcatcc catcagggag ccagtctgtg gagattttag
     2161 tcaaaaacgc cgccctccag ccggtcccca atgccgtagt cacgctcacc aactcggcgg
     2221 gcctgtttgt atccggcatc accaacgagc agggattgat cgcgcctcag tttcccggct
     2281 ccactctggg acccatcacc gtcaccgttc gcaaagacgg ttttaaagct tcggtgggac
     2341 agatcacttt gagctccggc agtggctttg tctatgcctc ccacttcgcc gatgatggat
     2401 atagcggaat ttctcagggt aatggagacg gttttgtgaa tggaggagag atcatcgagc
     2461 tctggctgag cctgagaaac acctcatcaa cggccagcga tgtatccctg gtagccagca
     2521 gcagcagtcc ctatgtcaac atcctggatt accacctgga tttcaatatc gtcccagccg
     2581 gacagaccgc tgagagcacc aatcccctcc agttttccgt gtcggcggat tgcccggacg
     2641 cttattcgct tatcatcagt ctggctgatt ccggtggcca gaatctctgc cagttcccgc
     2701 tcaatatcca gattcgaaac ggcaagccgg aaatcagctc ccacaccctg atcggagcgc
     2761 ctggcaacct tgtctatcca ggggatcagt atccgctcca gttcgatatc ctgaattccg
     2821 gaaccggagc tttgactggc cttagcgcga ccctgaggtc ctacgtcccg ttttgctcgg
     2881 tgcaggattc actgggaacc tttggagacg tgccggcagg caacccggga tcgaatgcca
     2941 gcgacacttt tactatcaat atatcacctc aggctttaac cgggatgcta atcccacttg
     3001 aactgagatt ccagaactcg cagggattta actatatcct atcctattcg cttacagtgg
     3061 gttcggtatc ggttacagat ccactgggcc aggatgctta tggctatttt atctatggca
     3121 tggaagatac ttcctacatg ctggccccca cctacacctg gatcggcatc gctcctgccg
     3181 aaggaggcag cggtacactg ctcgctctca cggaccctgg cgtaagcggt gatgaaggag
     3241 accagaccaa tgccagttcc atcaccacag tcaacctgcc cttcagtttt aaattctatg
     3301 gtacggacta cacccgggcc tcgatttcat caaacggctt tatcgctttt ggccagactc
     3361 aaaactcaga ttggcgaaac tggcgtctcc ctgggccagg tgggcccact ccaatgctgg
     3421 ccgccttttg ggatgatctc tgcctcaaca gcggtggagt ctatacctgg tataatgcca
     3481 gccagcacta ctatatcgtc gaatggtatg accttacgca tggcaaggac ctcagttcca
     3541 gagagacatt ccaggccatc ctctatgatc ctgtctatta ccccactctg agcggtgatg
     3601 gccagatcaa gctgcagtat aaagtgttca acaacatcga tgacagcgtg cagggttatc
     3661 cctatcccta tggcaatttc tgcagcatcg gcattaaaga ccagactgcc acgattggcc
     3721 tggagtatgc cttcaacaac agctttgctg ccgccgcagc tccactcggc aatgaaacag
     3781 ccctgttcat cagcaccgct cccactcccc agatcgcacc cagcctgacg cttggaacga
     3841 tatatgtagc cgacgcctca ggaaacaaca acggcctgct tgataccggt gagaactctg
     3901 gtctcaacat caggatcata aaccatggtc tcaatgccgc cactggagta agcgcaaccc
     3961 tcagcagcag cgatccctat cttaccatca ctcaaaacta ctccagctat ggctctgtgc
     4021 caggcagtgg cagtgctatg ccgctaaccc cttatcaggt gcaagtatcc gcctcggttc
     4081 ccgatggaca tcaggcagtt ctaaacttga atatcaactc cgctcagggc tcctggaacc
     4141 gggaatttca cctcactttg aacgcgcccc tgtttgaatt cggcactatg agcatccttg
     4201 accccagcgg caacaacaat ggagtgctgg acccgggtga aacggttacc ctcaggattg
     4261 tgctggccaa caatggcgga gctgaatcca gctcggggac cgcgtccgtc tccagcccca
     4321 ccccagggat cagcattatt aatggaagca caagtttcac ggctgtcggc agcagttcaa
     4381 ccactctgct gagctacacc gtggctgcca gttcgtccct cagcgagggt actcttgcca
     4441 cgctgagctt tcatgccggc gccggcaact actctgccga tgagaaccgc tatgttgaag
     4501 tcgggatgcc actcctggtc agcatcggta acggcacctc ggttcagagt tatccaatcg
     4561 acagatacta caactactcc gtccacgaat ccatctatct ggcttccgag atcggaggca
     4621 gtggtttgct caagtccatc ggcttttaca aaggctccgg gactgatgtt tccgccatcc
     4681 aatccgtgac catttacatg aaacatacca cagccacttc cctctcctcc ggaaactaca
     4741 gcaccaccgg atacactcag gtttataacg gtgacttccc aaatacagca acatctggct
     4801 ggatggaagt taacctgaat cccatgttca cctacagcgc ggatcagaac ctctcaatcc
     4861 tgatcgtgaa gaacttccag cagtacataa acaactatcc tcagtggaga tacaccacca
     4921 ctaccggcta ccgggccaga caggaaagaa gtgattcagc catgccgact tcacttagtg
     4981 catccgcgtc tcttcccaac ctccagatca ggatgttccc cgtccaggaa gtgctgtatc
     5041 caccccaaaa cctcacagcc caggccaccc accagaggat agacctcggc tggagcgctc
     5101 ccgtttccgg aactcccgtc tcctacaagc tctaccgcaa ttccagcctc ctgaccaccg
     5161 tcacagccct cagttataca gacctggctg tgaccaatgg aaccagctac agctactacg
     5221 tgaccgccct ttacagcaat cccaccgggg aatcggaacc ctccaacagc gtggatgtga
     5281 cgcccaccac cattgagttt gtcatcatcg ggaccggtac ctcggtaagc accaacggcc
     5341 aaaactctcc gataaatatc tccaacaaca gcactcatgg ccagctcgtc tacactgccg
     5401 cggaactcaa cgccgctgga gtcacaggac ctgtgttgat cagtgggctg ggattttatg
     5461 tggtcacctc tcccgatctg gccttgccca acttcatggt gaggatgaaa cacactaccg
     5521 cggttgatgg atcttcctgg catgacgcga atggctttgt taccgtctat tccaatgcca
     5581 gctatatgcc ctccgctggc ggttgggata tgctgatgtt cagcgctcct ttcgaatgga
     5641 acggagaaga caacctcgtg gtggataccg cttttggagt ggtggcccag gcttcccaaa
     5701 ccggtaccat gcaatatacc accattgcta acggctatcg ctttgcatgg agcaacacca
     5761 caaaccagag caacatcttc acgggcgggc ttgcagtaaa ccgtcgctac aatgttcgcc
     5821 tcatcctcca ggacatcccc tctggaccgt ccatcgaggt agaaccagcc accatcccct
     5881 ttggcaacgt acctgtcaac accagcagca tccagcagtt cagcatcgaa aacaccggcg
     5941 atacggtcc
//
LOCUS       DAOSFN010000142         5821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_12138_length_5821_cov_10.440687, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000142 DAOSFN010000000
VERSION     DAOSFN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12138_length_5821_cov_10.440687"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..750
                     /locus_tag="PLO35_08340"
     CDS             118..750
                     /locus_tag="PLO35_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH79718.1"
                     /translation="MAVKKDIRDLIAKAAEQEFLRHGFQGARTQAIADAAGTNKALLH
                     YYYQSKLKLYEHVLERQFASFFESLFSLVESDADFDTWLRGIIHNYLREVVTKPNFSR
                     FMLWEVSGHGLHLPGMIKKLMNNRGRDSQDVLDLIRTRLSGVGLDAYDPLQFLLNVLS
                     LCVFPSLARPIVSRVFGNQELLGAGFMQEREREVYLLITQGIFWRKGNES"
     gene            747..1622
                     /locus_tag="PLO35_08345"
     CDS             747..1622
                     /locus_tag="PLO35_08345"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH79719.1"
                     /translation="MNKIICILMTVALLTACAKKAKEPVWTSQVEARQFHVSTQTGGK
                     IISLKVKEGDAVQAGDTLAVLDARELEYGMEQLQAGLRELNAQEAIYRTQIASAEDDL
                     AYVEARQGRNEKLFEASVIPRQNLEDGQNLEAKARNLLKSTRQSLSVLDAKRASLAAQ
                     QKVLQKKLADCVVTSQASGTVETLFYSEGEVLPPLGQLAELSDLDDPEISIYVSEEWL
                     ARLKVGALFKLRAQGYRNPLDAELIRISNRAEFTPKTALTPDNRNVMVYALRLRVNNR
                     EGILKDGMPVDVSLK"
     gene            1619..2527
                     /locus_tag="PLO35_08350"
     CDS             1619..2527
                     /locus_tag="PLO35_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79720.1"
                     /translation="MNCVETAGLSIRYPDVTALDDLDLSIPAEMLYGIIGPDGAGKTS
                     LLRAICTLLPSSGGQISVMGMDVRKGQKRLRALIGYMPQRFSLYQDLSVEQNILFFGR
                     LFGVPKDKLRQRMEKLYKFSRLKPFANRLAGHLSGGMKQKLALSCALIHTPSLIVLDE
                     PTFGVDPVSRLEFWQMLHELKAEGISIVVSTPYMDEAQQCDRVSLLHRGRIMTEGPPD
                     EILARHPLRLYSLHGSHLQDVMRFMKALPGIDGLQMFGNEIHLSSKIPLDDRMFELWR
                     ASCPHLRSMKEIRPGMEDVFLTMMGS"
     gene            2524..3246
                     /locus_tag="PLO35_08355"
     CDS             2524..3246
                     /locus_tag="PLO35_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79721.1"
                     /translation="MIQTSALTKRFGDFVAVDHLDLRISAGEIFGFLGANGAGKTTSI
                     RMLCGLLRPSSGEARVNGFDVAHDSERVKRSLGYMSQRFSLYNDLKAIENLQFYAGIY
                     RVPFSVALDSTLPLIRLFGLEPSLKALTASLPVGFKQRLSLVCALVHDPPLVFLDEPT
                     SGVDPKARREFWEVINTLAGQGKTVIVSTHFMDEAEYCHRVVVMREGKAVALGRPSEL
                     KRQYEVDSMQELFIQIVGEVSA"
     gene            3239..4321
                     /locus_tag="PLO35_08360"
     CDS             3239..4321
                     /locus_tag="PLO35_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH79722.1"
                     /translation="MPEPRRVLAMAQKELYHILRDPRTLVILFLMPLLQLIIFGYAMN
                     MEIRDVPLAIEDQARNQASHKLIERFGSTSYFKLLPLPQWSDGPEALFRSRSALAYMT
                     IPKGFMESNPDLLFVIDASDPNSAQMIQSYATGIVNAMTTGTETKIDLRPMVMFNPDL
                     KSSFFFVPGLIAMILVMVCALLTSISISREKELGTMEQILVSPVSATEILLGKVMPYI
                     ALGLMDGLVILGVGMLLFRVPFRGEFTVFLGFTFLYVLTALCIGLLVSTLAKTQQAAM
                     MMALAITLLPTILLSGFMFPIASMPPILQKITYIVPARYYLQIIRGIMLKGNTIAQLY
                     QSGFILAGMSAGMLVLASRRFKLRLG"
     gene            4318..5451
                     /locus_tag="PLO35_08365"
     CDS             4318..5451
                     /locus_tag="PLO35_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH79723.1"
                     /translation="MSGLSVIGSFLKKEFRQILRSREMLIVLFALPAMQLLVMGFAVT
                     NEVKNLTLSFFDYDNSPLSRNLCASFSQGDRFRVVPVSQAGKPEELLFRWQSKVVIVI
                     PKGFARDVYSGRQVSLQVISDGIDGNTAALATTYAGGYLAEFAQKLEARKDLGWRALK
                     KAPDKRINLISRMSFNPDLKSSSNIIPGIIAVLVTVTSMLLSAISLVKEKELGTLEQL
                     MVTPVKKHQLLAGKLIPYLLITLLQMQVAMLLARIIFGVTVSGSYAVLLLFSLVYLFT
                     TLGLGILISTRVQSQQQAMFFSWFIMVVAILLSGFFVPVRNMPEGIRLIAWFNPLSHY
                     MTVLREIVVKGSGLIQLGREFAVLLGSGALIFGISVISFRKRV"
BASE COUNT         1304 a         1558 c         1670 g         1289 t
ORIGIN      
        1 gcacgcgccc gactggaatc cgacgctacg gacccggcaa aaatagattg acatcctatg
       61 gcgttcccca gtatttgacc atatggttaa acacactttt ataaaggact gctatttatg
      121 gcagtaaaga aagacatcag agacctgatc gccaaagcgg cggagcagga atttctgcgt
      181 cacggctttc agggagccag gactcaggcc atcgcggacg ccgccggcac caataaagca
      241 ctattgcatt actactatca gagcaagctg aagctctatg aacacgtgct ggaacggcag
      301 tttgcctcct tttttgagag cctgttttcc ctggtggaga gtgacgcgga tttcgacacc
      361 tggctcaggg gcatcatcca caactacctg agggaagtgg ttaccaagcc caacttcagc
      421 cgctttatgc tttgggaggt cagtggccat ggcctccacc tgcccggcat gatcaagaag
      481 cttatgaaca accggggacg cgacagccag gacgtcctgg acctgatcag gacgcgtctg
      541 agcggtgtgg gcctggacgc ttacgatccg ctccagtttc tgctcaacgt gttgtcgctc
      601 tgtgtgtttc ccagcctggc caggccgatc gtgtctaggg tgtttggaaa tcaggagctt
      661 ttgggagcgg gctttatgca agagcgggag cgggaagtct atctgctcat cacccaggga
      721 attttctggc ggaaaggaaa tgaatcatga acaagatcat ctgcatcctg atgacggtgg
      781 cgctgctgac agcctgcgcc aaaaaggcca aagagcccgt ctggaccagc caggtggagg
      841 ccaggcaatt ccacgtttcc acccaaaccg gcgggaaaat catctcgctc aaggtgaaag
      901 agggagacgc ggtccaggct ggagacacgc tggcggtttt ggacgctcgg gagctggaat
      961 atggcatgga acagctgcag gcagggctgc gggaattgaa tgcccaggag gccatctacc
     1021 gcactcagat agccagcgcg gaagatgacc tggcctatgt ggaagccagg cagggccgca
     1081 atgaaaagct gtttgaggcc tccgtgatcc cgaggcaaaa cctcgaagac ggccagaacc
     1141 ttgaggccaa agcccgcaac ctgctcaagt ccacccgtca aagcctgagc gtattggatg
     1201 ccaagcgcgc ctccctggcc gcccagcaaa aggtgctgca aaagaagctg gcggattgcg
     1261 tggtgaccag ccaggcctcc ggaacggtgg aaaccctgtt ttacagtgaa ggcgaggtgc
     1321 tgcctccctt aggccagctg gcggaactga gcgatctgga tgatcccgag atcagcatct
     1381 atgtatcgga agagtggctg gccaggctga aagtcggtgc cctgttcaag ttgcgcgccc
     1441 aaggctatag aaatcccctg gatgcagaac tgatcaggat cagcaaccgg gccgagttta
     1501 cccccaagac agctttgaca ccagacaacc gcaacgtgat ggtttacgcc ctgcgcctca
     1561 gagtaaacaa tcgggaaggc atcctcaaag acggcatgcc ggtggatgta agcctgaaat
     1621 gaactgcgtg gaaacggccg gcctgagcat ccgctatcca gacgtgacgg cgctggacga
     1681 tctggacctg agcatccctg cagagatgtt gtatgggatc ataggaccgg acggagctgg
     1741 aaagaccagc ttgctgaggg cgatttgcac tctgctgccc agctcgggag gccaaatcag
     1801 cgtgatgggg atggacgtcc gcaagggcca aaagcggctc cgagcgctga tcgggtatat
     1861 gccccagagg ttttcgctgt atcaggacct ttcggtggag cagaacatcc tgttctttgg
     1921 ccggctgttt ggcgtgccca aagacaagct ccgccagagg atggaaaagc tctacaagtt
     1981 ctcacggctc aagccttttg ccaatcgact tgccggccat ctgagcggtg gcatgaagca
     2041 aaagctggcc ctgtcctgcg ctctcatcca cactcccagt ctgatcgtgc tggacgagcc
     2101 cacctttgga gtggaccccg tttcccgcct cgagttttgg cagatgttgc atgagctgaa
     2161 agcggagggg atcagcatcg tggtctccac tccctatatg gatgaggccc agcagtgcga
     2221 ccgggtgagc ttgctgcacc ggggcaggat catgacagag ggcccgcccg atgagatcct
     2281 ggcgcgccat cccctgcgcc tctatagctt gcacggcagc cacttgcagg atgtaatgag
     2341 gtttatgaaa gctctgcccg ggatagatgg cctgcagatg tttggcaacg agattcacct
     2401 cagcagcaag atcccgctgg atgatcggat gtttgagctg tggcgggctt cctgtccaca
     2461 cctgagatct atgaaagaga tcaggccggg catggaagac gtgtttctga ccatgatggg
     2521 atcatgatcc agaccagcgc gctcaccaaa cgttttggcg attttgtggc ggtggaccac
     2581 ctggacctca ggatctccgc gggtgagata tttggctttt tgggtgccaa cggggctgga
     2641 aagaccacct ctatccgcat gctctgcggt ctgttgcgtc ccagcagcgg tgaggccagg
     2701 gtgaatggat tcgacgtggc ccatgacagc gaaagggtga agcgcagcct gggctatatg
     2761 agccagagat tctcgctcta caacgatctg aaggcgatcg aaaaccttca gttttatgcc
     2821 gggatctacc gggtcccttt ctcagtggct ttggatagca ccctgccttt gatcaggctg
     2881 tttggcctgg aaccgagtct gaaggcccta accgcttccc tgccagtggg attcaagcag
     2941 cgcctcagtc tggtctgcgc tttggtccac gatccgcctc tggtgtttct ggacgaacct
     3001 acttccgggg tcgatcccaa ggcccgcaga gagttttggg aagtgatcaa cacccttgcc
     3061 gggcagggaa agaccgtgat cgtgagcacc cattttatgg atgaggcgga gtattgccat
     3121 cgggtggtgg tgatgcggga gggcaaagcg gtcgcgctgg gcagaccgtc tgaactgaag
     3181 agacagtacg aggtggacag catgcaggag ctgtttatcc agatcgtggg agaagtaagt
     3241 gcctgagccc agacgcgtcc tcgccatggc ccaaaaggag ctgtatcata tcctgaggga
     3301 cccccgcacc ctggtgatcc tctttctgat gccgctcttg cagctgatca tctttggcta
     3361 tgccatgaat atggagatca gggacgtgcc tctggcgatc gaggaccagg cccgcaacca
     3421 ggcctctcac aagctgatcg agcgctttgg cagcaccagc tatttcaagc tgcttccctt
     3481 gcctcagtgg agtgatggtc cggaagcctt gttcaggagc aggtccgcac tggcctatat
     3541 gaccattccg aaagggttta tggaatccaa tcccgatctg ctgtttgtga tcgatgcctc
     3601 cgaccccaac agcgctcaaa tgatccaaag ctacgccacc gggatagtaa atgccatgac
     3661 gacgggcacg gaaacaaaga tagacctgcg tcccatggta atgttcaatc cggacctcaa
     3721 gagctccttc tttttcgtgc cgggcctaat cgccatgatc ttggtgatgg tctgcgcctt
     3781 gctcacgagc atatccatca gccgggaaaa ggaacttggc accatggagc agatcctggt
     3841 ctctcctgtt tcggccacgg agatcctgct gggcaaggtg atgccctata tagcattggg
     3901 gctgatggac gggctggtga tcctgggagt gggtatgctg ctgtttcgtg ttccgttccg
     3961 gggcgagttt accgtgtttc tgggcttcac cttcctatat gtgcttacgg ccctgtgcat
     4021 tggcttgctg gtgtccaccc tggccaagac tcagcaggcg gcgatgatga tggcgctggc
     4081 gatcacgctg ttgcccacca tcctgctatc cggcttcatg tttcccatcg ccagcatgcc
     4141 tcccatcctg cagaagatca cctacatcgt gccagcccgc tattatctgc agatcatccg
     4201 cgggatcatg ctgaagggca acacgatagc ccagctctac caatccggct tcatcctggc
     4261 tggcatgagc gcgggaatgc tggtcctggc ctccagacgc tttaaactga ggctgggatg
     4321 agcggactga gtgtaatcgg cagcttcctg aagaaggagt tcaggcaaat cctccgcagc
     4381 cgggaaatgc tgatcgtgct gtttgctctg ccagctatgc aactgctggt gatgggattt
     4441 gccgtcacca acgaggtcaa gaaccttacg ctcagcttct ttgattacga caacagcccc
     4501 ctcagccgta atctgtgcgc ttctttcagc cagggagacc gctttcgggt ggtgccggtc
     4561 tcacaggccg gcaaacctga ggaactgctc ttcaggtggc agagcaaggt ggtgatcgtg
     4621 atccccaaag gctttgccag agatgtctat tccggccgcc aggtcagcct gcaagtgatc
     4681 agtgacggga tcgacggcaa caccgcggcc ctcgccacaa cctatgcggg aggctacctt
     4741 gcggagtttg cccagaaact ggaagcaagg aaagaccttg gatggcgggc tctgaagaaa
     4801 gctccggata aacggataaa cctgatctcc cgaatgagct tcaatccgga cctcaagagc
     4861 agcagcaaca tcatccccgg gatcatagcg gttttggtga cagtgacttc catgctgctc
     4921 tcggccatca gcctcgtgaa agaaaaggaa ttgggcactc tggaacaact gatggtgact
     4981 ccggtgaaaa aacatcagct cctggctggc aaactgatcc cctacctgct gatcactctc
     5041 ttgcagatgc aggtggcaat gctgctggcg cgcataatct ttggcgtcac ggtctcggga
     5101 tcatacgctg tgctgctgct gttttcgctg gtgtacctct ttaccacgct gggcttgggg
     5161 atcctgatct ccacccgcgt gcagtcacag cagcaggcga tgttcttttc ctggtttatc
     5221 atggtggtgg ccatcctgct ttccggcttc tttgtcccgg tgcgaaacat gcctgaagga
     5281 atcagactga tagcctggtt caatcctctc agccattaca tgacggtcct gagagagatc
     5341 gtggtgaagg gcagcggctt gattcagctt ggcagagaat ttgccgtcct gcttggcagc
     5401 ggagcgctga tctttgggat cagcgtgatc agcttccgga agagagttta aaccacggaa
     5461 gccttcccgg gcggagaaga gccatacggt aaggatttat accctcactg aatacttcaa
     5521 atttcatcac caaaacttca aatgtccaag tccaaaagat gatgaaccag acgattaggg
     5581 cgtttgaaat atgctaattt ctttaagatc attgtattgt atatatggcg caagattcct
     5641 gataatccct gggaatgtcc aagtgcctca aaaacgatgg ggaatttgaa gttttggtga
     5701 taaaaagagg tcgctcatca tggtactgtc caagtccaac aaagcagatg tgtctaacca
     5761 tatcgtattt ttcaacaatg tgatagcatc aattgtccaa gtgagcgcaa tttgaagttt
     5821 t
//
LOCUS       DAOSFN010000143         5657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_12625_length_5657_cov_8.789539, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000143 DAOSFN010000000
VERSION     DAOSFN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12625_length_5657_cov_8.789539"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(780..1832)
                     /locus_tag="PLO35_08370"
     CDS             complement(780..1832)
                     /locus_tag="PLO35_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOH79724.1"
                     /translation="MKILEAIQDPNSELALLLDSLYDAVYIVDRERVILYWNHAAEQI
                     TGFSAAEVLGHSCKDGILNHIDENGKLLCYEGCPLLYAMENAGNTSAKVYPCHKQGHR
                     FPVETHVTSILDEEGTAVAAIEVFRDITHQEEFRIMQEKFNGLIRKYVSSVTFDEVRE
                     QAKGKAGSRASRIVDLTVLYLDVVNFTGFSERNDSSAVMEMLNELFGICDVITRECLG
                     DIDKFIGDAIMAIFNDANDAVRASVKILQEALPQMNQSRQQRGLQEISVRIGINSGLV
                     MQGEVGTLDRRDLTVIGDAVNIAARVEKSSLPNRLMISEASHARLKADLRPKFEFHHE
                     EQLRGKNASVRLYLLQ"
     gene            complement(1842..2870)
                     /locus_tag="PLO35_08375"
     CDS             complement(1842..2870)
                     /locus_tag="PLO35_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOH79725.1"
                     /translation="MNFTRLAFMLLGLITLLSSGACASVKTNVFPLGDSYLKEEQLYS
                     RLKEIAALNPGLAKLRVIGFSGTNEIPLYALEIGMASAPRKILIIGQHHGDEVLGVQL
                     AVALANELVTKWKKDSRWERILDEFQFWIVPTINPEGWRSVSRGEYQWKRKNNRDTDK
                     NKRFDLRTDGIDLNRNYPVFWADDPPVPAINPYYKGERPASEPEIQAIMKLADTHQYD
                     LAIFYHSSASGAYSEKIYLPAWVVTDEEQKSRIVKLMDFAKDYAALIKRDYQKGHYQV
                     GEGNSSRVGNARNYFFHTHGTDAMLVEVGGINSSGVSVIHPPEAMIKKIVDRHVQALR
                     KVLYKRSG"
     gene            complement(2888..3925)
                     /locus_tag="PLO35_08380"
     CDS             complement(2888..3925)
                     /locus_tag="PLO35_08380"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOH79726.1"
                     /translation="MKKTIFSFILATLVLVLGAQGNYYNGVLTLTGNSLLTGLRALIS
                     TNTYSSYDGAKVYLFQDLENTNGNVTCIYTGEVYYVGYNYTGSNNPNTEHTYAQSWFS
                     SPSTSIKKADLHHLFPSNSVVNSSRGNFPLYTVANHSSANVYYNDTPWQSYRGNSSTG
                     YTVFEPADETKGNIARALLYFYTRYNDPLTQQNINMLPVLVDWSNQDPPDTWEITRNQ
                     GVYSFQGNRNPFVDHPEFVNRIWGPVDEDDPLLPAVSELYLNGVWPNPFSQNARISLQ
                     SKSSRSANLSVYDIRGRKVKSTSFNLVPGVNELGWDGKDEAGQELGSGIYLLRISSGE
                     LNLSARLVLQR"
     gene            complement(3930..4451)
                     /locus_tag="PLO35_08385"
     CDS             complement(3930..4451)
                     /locus_tag="PLO35_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008801347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOH79727.1"
                     /translation="MANEDNFVEIQRVANAIARCFREGGKCIIFGNGGSMCDATHFAE
                     EFTGRFRNERPALPCIAISEPSHLSCVGNDYGFEEVFSRGVEAFARPDDFVIGISTSG
                     NSPNVIRALQKAQDLGCFTFALLGKDGGLLKDACDFELIVPAATSDRAQEIHTLILHI
                     IIECVEIELYPEL"
     gene            complement(4508..5587)
                     /gene="add"
                     /locus_tag="PLO35_08390"
     CDS             complement(4508..5587)
                     /gene="add"
                     /locus_tag="PLO35_08390"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOH79728.1"
                     /translation="MKIKLTRELIHKLPKTDLHVHLDGSVRIPTILDLARQHNIKLPT
                     MDAVELEKLIVCGEHTTSLDDYLRGFHIVNLVLQTKEGLRRAAYELAEDAAAENVRYM
                     EVRYSPILHTLGGLKLTEISQAVIDGLKHAERDFRIKTGVIICGIRNMDPTTSLKLAE
                     LAIAFKNKGVIGFDLAGGEYNHPAKDHKEAFDLALKNNLNITIHAGEAYGPESIHQAL
                     HYCGTHRIGHGTRLVEDGDLLNYVNDHRIPLEICIKSNFHTKAVPDIRSHPIDFYIDY
                     GLRVTINTDNRTISNTTVTDEYMLAINELKLDYSTIKYVILNGFKSAFAPYKERVRLI
                     NDTLKEFEEIEEAELKTKVKVKENL"
BASE COUNT         1189 a         1399 c         1638 g         1431 t
ORIGIN      
        1 ggagggggtg gccctcttca agcggaatgg gtggctcagt aggttcggaa tatcctgatt
       61 agcattacgg agtcattacg gactcattac gggtgaagtc cgtaatgagt ccgtaatagg
      121 atgcggggag gagtaagaga tcaagtaata atgctatgca ttatatgaca ttagaggttt
      181 ttcacaggga gcgaatcacg cttctggaac tcaaagcgac gcaccatgat ccccatgcat
      241 ttccatgttc attgacgctg tggaacttcc agcaactcac gcataaatag accgttgcgc
      301 atagctgtaa cactggcatt agagcttgtg aaaaaactac atggagagct gcgatcgcac
      361 gagtcccagc gggacggcag tttagataga ttagtgttgc aagatagatg gttgagtcct
      421 gtagggacgg tacaacagat gcgtatagtg ttgaatatca ggcgtataat gtattgtgac
      481 agcaatctaa actgtcgtcc cgatgggact ccaattattg ataggagcac gtgtatctat
      541 ctaaactatc gttcctacgg aacttaaaat gccattttat tcacaagctc taaagccagt
      601 gatacagatt attcaacggc gtcataaatg gtaaccctta atctctgtgc tccgtagtgc
      661 tcagttttgc cagtgtttac tgaggaaagt cccggcagcc cgcaatctta ctgccttcta
      721 tagctttagc gcggctgcgt gaactgcggc cggagcggaa gctttcttat gcaggtcgtt
      781 tactgcagca aatacagcct tacgctggca tttttgcccc ggagctgctc ttcgtgatga
      841 aactcgaact tgggcctcag atcggctttc agcctggcgt ggctggcctc ggagatcatc
      901 agccggtttg gcaggctgct tttttccacc cgggcggcga tgttgacggc gtctccgatc
      961 acggtgaggt cgcgccggtc cagggttcca acctcgcctt gcatcaccag accgctgttg
     1021 atgccaatgc gcacactgat ctcttgcagg cctcgctgct ggcgggattg gttcatctgg
     1081 ggcagggctt cctgtagaat cttaactgag gcccggacgg catcgttggc atcgttgaag
     1141 atcgccatga tggcgtcccc gataaacttg tctatatctc ccagacattc acgcgtgatg
     1201 acgtcgcaga ttccgaagag ctcgttgagc atttccatca ccgcgctgct gtcgttgcgc
     1261 tccgaaaagc cggtgaaatt gaccacatcc agatacaata cggtgaggtc cacgatcctg
     1321 gaagcccggg atccggcctt gcctttggcc tgttcgcgca cttcgtcgaa ggtgacggaa
     1381 gacacatatt tgcggatcag gccgttgaac ttctcctgca tgatgcggaa ttcttcctga
     1441 tgggtgatat cgcgaaagac ctcgatggcg gccacggcag tgccttcctc atccaggatg
     1501 ctggtcacat gggtctccac cggaaagcga tgcccctgtt tgtgacaggg atagaccttg
     1561 gcggaggtgt tgccggcgtt ctccatggca tacaaaagcg gacatccttc gtagcagagc
     1621 agtttgccgt tttcgtctat gtggttgagg atgccgtctt tacagctatg gcccaggacc
     1681 tcggccgcgc tgaagccggt gatttgttcg gcggcgtggt tccaatagag gataacgcgt
     1741 tcgcggtcaa cgatatagac agcgtcatag aggctgtcca gcaagagggc cagctcgctg
     1801 ttggggtctt ggatggcctc gaggatcttc atgggttctc cttatccgga gcgtttatac
     1861 agaacctttc tgagggcctg gacatgtctg tcaactattt tttttatcat ggcttcagga
     1921 ggatggatga cgctgacccc ggaggagttt ataccaccca cttccaccag catggcatcc
     1981 gtgccgtgag tgtgaaaaaa gtagttgcgg gcattgccca ctctggagct attaccttct
     2041 cccacttgat aatggccttt ctgatagtca cgcttgatca gggccgcgta gtctttggca
     2101 aagtccatta gtttgacgat cctggacttt tgttcctcgt cggtaactac ccaggccggc
     2161 agatagattt tttcgctgta ggctcccgag gccgagctgt gatagaagat ggccagatca
     2221 tactgatggg tgtcggccag cttcatgatg gcctggattt ccggttcgga ggcgggcctt
     2281 tcgcctttgt aataaggatt tatagcaggc acgggaggat cgtcggccca gaaaacggga
     2341 tagttgcggt tgagatcgat gccgtcggtg cgtaggtcaa aacgcttgtt tttatctgta
     2401 tcgcggttgt ttttgcgctt ccattgatat tcgccgcggc tcacgctgcg ccagccttcc
     2461 gggttgatgg tgggcacgat ccaaaactga aactcatcca ggatccgctc ccagcgggag
     2521 tcctttttcc acttcgtgac cagctcattg gcaagcgcca cagccagttg cacacccaac
     2581 acctcatcgc cgtggtgctg accgatgatc aggattttgc gcggcgcgct ggccattccg
     2641 atctcgagag catagagggg aatctcgtta gtgccggaaa agccgatcac tctcagcttg
     2701 gccagacctg gattgagggc cgcgatctct ttgagccggc tgtaaagctg ctcttctttc
     2761 agataggaat cgcccagcgg aaacacgtta gtctttacgg aggcgcaagc ccctgaactg
     2821 aggagcgtga tcagccccag cagcataaag gccagcctgg tgaagttcaa tctggctatc
     2881 cctccggtta gcgctgcagc accagcctgg cgctcagatt cagctcaccg gagctgatcc
     2941 tcagcagata aatcccgctg ccaagctctt gtcccgcttc gtcttttccg tcccagccca
     3001 gttcattgac gccggggact aaattgaagg atgtgctttt taccttgcgg cctctgatgt
     3061 catagacgct caggttggca gagcgggagc tcttgctctg gaggctgatc ctggcattct
     3121 gtgagaaggg attgggccac acgccgttca gataaagctc tgaaacggct ggcaacaagg
     3181 gatcgtcctc atccacagga ccccagatgc ggtttacgaa ttccggatgg tccacaaagg
     3241 gattgcggtt gccctgaaag ctgtaaacac cctggttgcg agtgatttcc caggtgtcgg
     3301 gggggtcctg gttgctccag tccaccagca ccggcagcat attgatattt tgctgggtga
     3361 ggggatcgtt gtagcgggtg taaaaataga gcaaagctct ggcgatgttg cccttggtct
     3421 catcggccgg ttcaaacacg gtgtagccgg tggagctgtt gcctctgtag ctctgccagg
     3481 gagtatcgtt gtaatagacg ttggcagagg agtgattggc cacggtgtag aggggaaaat
     3541 tgccccggga gctgttgacc acggagttgg agggaaagag gtgatgcagg tcggcctttt
     3601 tgatcgatgt ggaggggctg gaaaaccagc tctgggcgta ggtgtgctcg gtgttggggt
     3661 tgttggagcc ggtgtagttg tagcccacat aatacacttc tccagtgtag atgcaggtca
     3721 cgttgccgtt ggtgttttca aggtcctgaa agagatagac cttggccccg tcgtagctgc
     3781 tgtaggtgtt ggtgctgatc aaagctctca ggccggtcag cagagagttt ccggtgaggg
     3841 taagcacccc gttgtaatag tttccctggg ctcccagcac caggaccagg gtggcaagaa
     3901 tgaagctgaa tatggtcttt ttcatggttt tataactctg gatatagttc tatctccaca
     3961 cattcgatga tgatgtggag gatgagggtg tggatttcct gcgcccggtc tgaggttgcg
     4021 gccggcacga ttaattcaaa atcacaagca tctttgagca ggccgccgtc cttgcccagg
     4081 agggcaaagg taaagcagcc gaggtcctgg gctttttgca gggcccggat cacgttgggt
     4141 gagtttccac tggtggagat gccgatcaca aagtcgtctg gacgggcaaa ggcttccacc
     4201 ccgcgggaga aaacctcttc aaagccgtaa tcgttgccca cacagctgag atgggagggc
     4261 tcggaaatgg caatgcaagg cagggcggga cgctcgttcc ggaagcggcc ggtgaattcc
     4321 tcggcaaaat gagtggcatc gcacatggag ccaccgttgc caaagatgat acacttgccg
     4381 ccttctctga aacagcgcgc gatggcattg gccacccgct ggatctccac aaagttgtct
     4441 tcattggcca taaacccgct cagggtctga tgggccagct caaaggagcg cttgatggtg
     4501 ttgtacatca gaggttctct tttaccttca ccttggtctt tagctcggct tcttcgatct
     4561 cctcaaactc cttgagggtg tcgttgatca gcctcacccg ctctttatag ggggcgaagg
     4621 cactcttgaa accgttgagg atcacatatt tgatggtgga gtaatccagc ttgagctcgt
     4681 tgatggccag catgtattcg tcggtgacgg tggtgttgga aatggtgcgg ttatcggtgt
     4741 tgatggtcac ccgcagaccg tagtcgatat agaaatcaat gggatggctg cggatgtcgg
     4801 gtaccgcttt agtgtggaaa ttgctcttga tgcagatttc cagggggatg cggtggtcgt
     4861 tcacgtagtt gagcagatcg ccatcctcca ccagcctggt gccgtgaccg atgcgatggg
     4921 tgccgcaata gtgcaaggcc tgatggatgc tttcggggcc ataggcttcc ccggcgtgaa
     4981 tggtgatgtt gaggttgttt ttgagagcca ggtcaaaggc ttccttgtgg tccttggcag
     5041 ggtgattgta ttcgcctcca gccaggtcaa agccgataac tcccttgttt ttgaaggcga
     5101 tggccaactc ggccagtttc aaagaggtgg tggggtccat attgcggatc ccgcagatga
     5161 tcacacccgt cttgatccgg aaatcacgtt cggcgtgttt gagcccgtca atcacggcct
     5221 gggagatctc agtgagcttc aatcctccca aggtgtgcag gatggggctg taacgcactt
     5281 ccatgtagcg cacgttttcg gccgcggcgt cttctgccag ttcataagcg gcccgcctga
     5341 ggccttcctt ggtctgcaat accaggttta cgatatgaaa tccccgcaga taatcatcca
     5401 gactggtggt atgttctccg cagacgatca gtttttccag ctcgaccgcg tccatagtgg
     5461 gcaatttgat attgtgctgc ctggccagat cgaggatagt ggggatccgg acactgccgt
     5521 ccagatgcac gtgcagatcg gtcttgggca gcttgtgaat gagctcacgg gtcagtttga
     5581 tcttcatttt ctttcatcct tttaatttca aggcaagata agctgagggg gacttgttgt
     5641 caactagaaa gtgagag
//
LOCUS       DAOSFN010000144         5654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_12635_length_5654_cov_9.054295, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000144 DAOSFN010000000
VERSION     DAOSFN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12635_length_5654_cov_9.054295"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..76)
                     /locus_tag="PLO35_08395"
     tRNA            complement(1..76)
                     /locus_tag="PLO35_08395"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Ala,seq:ggc)
     gene            complement(325..1809)
                     /locus_tag="PLO35_08400"
     CDS             complement(325..1809)
                     /locus_tag="PLO35_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOH79729.1"
                     /translation="MDQILIGKGDSQVFLDPAFGNRHGLVAGATGTGKTVTLLVLAEG
                     FSRMGVPVFMADVKGDVAGLAMPGTMNDKLAARVAQIGVEGYTQEGSPLIFWDVFGSS
                     GHPVRTTISEIGPALLSRILDLNDVQSGTLEIVFKLADDEGLMLLDLDDLRSLLSFVA
                     DNKSEISKAYGLVSPASIAAIQRSLLGLEREGAEQLFGEPALELADLMRTDLSGRGVI
                     SVLAADKLVLSPKLYSSLLLWLLSELYENLPEVGDLDKPRMVFFFDEAHLLFDDAQPS
                     LIKKVEQVVRLIRSKGVGVYFCSQFPDDIPSEILGQLGNRFQHALRAYTPRDQKAVRT
                     AAETFVPNPRLKVADVISQLGVGEALVSTLMTKGVPMPVEKTVISPPRCRMGSITDQE
                     RAAIIARSPVGIKYDRAVNRESAHEILQRRANERVENQEQEASAVKTKAEPERSAVGE
                     LLWGTKKRQGLVETMAKQTARTVGSQVGRSIIRGIMGSIFGGKR"
     gene            1984..3273
                     /locus_tag="PLO35_08405"
     CDS             1984..3273
                     /locus_tag="PLO35_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOH79730.1"
                     /translation="MKKIGLTDQQVIALVDCNNFYVSCERVFDPSLEGKPVAVLSNND
                     GCMVSRSAEIKALGIPMGAPGFKYEGLVRQHGGTLLSSNYALYGDMSARVMETLSHFA
                     PEVEIYSIDEAFLVLTGLRLKDLEEYGRKIRKTVTQWTGIPVSVGISTSKTLAKVANH
                     WAKRVPGFKGSLALMDEDRIAKALQKTPVGEVWGIGRQYEKFLIQNGINTAWQLRNAN
                     EKFVDHYMTVVGHKTVLELQGFSAIEIDEVPSPKKSIVVSRSFGKQVESLAELEEAVS
                     TYITRAAEKLRVQDSVAGRMMVFLSTNRFKEGPQYSNSLQTVLSPPSAYTPDLIRIAL
                     ELLRELHLPGFEYKKAGVMFSEIIPSGDVPLSFIETNYLDDRRKVLMDTVDKINRRSG
                     RDTVFYASSGVKKEWQMKRARLSPAYTTRWSDLPRAK"
     gene            3270..3881
                     /locus_tag="PLO35_08410"
     CDS             3270..3881
                     /locus_tag="PLO35_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOH79731.1"
                     /translation="MSDWHTLEQACEAKITIHRSRFIAFIVPVSDPAQAASEIAVRSH
                     EFEDATHNCYAWVTGFKREQSYYSDAGEPAGTAGKPILNALLSAGLTNVAATVTRYYG
                     GVKLGVRGLIEAYSQSVEEALKTASLVPAIPMSTIWITSDYSDAESAQRLVQKLGGGV
                     VDIRYEARVETILSLPERSLAEFCEFLDGRGRQNGLVYRLKEQ"
     gene            3883..4764
                     /locus_tag="PLO35_08415"
     CDS             3883..4764
                     /locus_tag="PLO35_08415"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOH79732.1"
                     /translation="MRILIIAMLLLVIIGTLTAQDARIISTRNGKTVSIAQLAKELKK
                     YDVVFFGEFHDDATLHALQAELLPLMHKAGKRLIVSFEMFERDVQPDLDAYLAGSVTE
                     EQFLQSSRPWSNYGTDYRPLLEFARLKNLKAIAANIPRRLAGQVARNGTAALDDLSND
                     DKVLMALEISAPEGKYKERFMATMKANGMHASPNDANMLERLYYAQCMKDDTMAESLA
                     QYLERYPRHRIVHYNGDFHSREYLGTVERLRSRVPKARIAVLSPHYASLPLPETPSEL
                     ADYLIVIPEKPEQNQED"
     gene            4769..5590
                     /gene="amrB"
                     /locus_tag="PLO35_08420"
     CDS             4769..5590
                     /gene="amrB"
                     /locus_tag="PLO35_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOH79733.1"
                     /translation="MIRKATHAGKFYPRFGYAIISQLAGWTAESRKAPHTEHSLGLIV
                     PHAGYMYSGAIAARAYHHISEEGFDAFVVLHPSHHGGNFDYSVSAFQEYETPLGNIRL
                     DTECYAALTSGDEPSDLWLRYHEQEHSMEIQLPMIKYFFPDARICPVMLGRQNLNNSR
                     RLADRISYLLTQTSRRVAIIASTDLSHYHDAASAETKDKLLAKLVQEQAIDDLWDLIN
                     DGDCEACGIGAILTLLCLGSNVPGAKMQVLEYTHSGLVSGHNQQVVGYLAAKLWR"
BASE COUNT         1278 a         1644 c         1509 g         1223 t
ORIGIN      
        1 tggtggagca taacgggatc gaaccgttga cctcatgact gccagtcatg cgctctccca
       61 gctgagctaa tgccccaaat tgctggtgca agtttcgtgg ctcacttccg ccaaaggccg
      121 tttttttgtc aagcagataa ttcacgagat agcgtaagat gctgtaaact atagctgtct
      181 ttgtctgatt cactccatcc tgcgtcttct ctgcattgtc gagcccgagt ccagccggac
      241 catatcggac ccatccccaa caaatgcggc ttgggtgggg catggtatgc cgcggacagg
      301 gtgaaagggt taagggccaa tgccttagcg cttgcctcca aagatgcttc ccatgatgcc
      361 gcggataatg gaacggccca cctggctgcc caccgtcctg gccgtctgtt tggccatggt
      421 ttccaccagg ccctggcgtt ttttggtgcc ccagagcagt tctcccaccg cgctgcgttc
      481 aggttctgct ttggttttga ccgcgctggc ttcctgttcc tgattctcta cccgttcgtt
      541 agcccggcgc tgcaggattt cgtgggccga ttccctgttc accgctctgt catacttgat
      601 cccgacaggg ctgcgggcta taatggcggc acgttcctga tcggtgatgc tgcccatgcg
      661 gcaacggggt ggactgatca ccgttttctc caccggcata ggtactccct tagtcatcag
      721 ggtggagaca agcgcttctc ccactcccag ttgcgaaatc acgtcggcca ccttgagcct
      781 gggattgggt acaaaggtct cggccgctgt cctgacggcc ttctgatctc tgggagtgta
      841 agcgcggaga gcgtgttgga agcggtttcc caactggccg aggatttcgg aagggatgtc
      901 gtctgggaac tgggaacaga aatagactcc cacgcccttg gagcggatca ggcgcaccac
      961 ctgctccacc tttttgatca gcgagggctg cgcgtcgtca aagagcaaat gggcttcgtc
     1021 gaagaagaat accatgcggg gtttatcgag gtctcccact tccggaaggt tttcatagag
     1081 ctccgaaagc agccataaca gcaagctgga atagagcttg gggctcagca ccagtttgtc
     1141 cgcggccagc acactgatga cgccgcgacc gctcaggtcc gtcctcatca ggtctgccag
     1201 ttccagggcc ggctctccaa acagctgctc ggcaccttcc cgttccaggc ccagcaggga
     1261 acgctgaatc gcagctatgg aagcgggact caccaatccg taggctttgg aaatctcgct
     1321 tttgttgtcg gccacaaagg agagcaggct tctgaggtcg tcgaggtcca gcagcatcaa
     1381 tccttcatcg tcagccagct taaagacaat ttccagggtg ccggactgca cgtcgttgag
     1441 atcgagaatg cgggaaagga gagcaggtcc gatctcgctg atcgtggtgc ggaccggatg
     1501 cccggagctt ccaaacacat cccaaaagat cagaggactg ccttcctggg tataaccttc
     1561 cactccgatc tgggccactc tggccgccag cttgtcattc atggtacccg gcatggccag
     1621 tcccgccacg tcgcctttga cgtctgccat gaagacgggc actcccatgc gggaaaatcc
     1681 ttccgccaac accaacagcg tcaccgtctt accggttccc gtggcccctg caaccaagcc
     1741 atgcctgttt ccaaaggcgg ggtccagaaa gacctggctg tctcctttgc cgatcaatat
     1801 ctgatccatt aaaacctcct gggacgcgtc ctgaaaggga acgcttatct ttcagcccag
     1861 tctttatgct gaaaagaaaa cgtcaagaca tatttctgga gtctgttaac tcaggcacct
     1921 cataaccacg aaagcggggc ttgacaaaag aggccttcca atataccctg caaccgaggt
     1981 accatgaaaa aaatcggctt gactgaccag caggtgatcg cgctcgtgga ttgcaacaac
     2041 ttctacgtgt cctgcgaacg cgtgtttgac ccttctctgg agggcaaacc ggtggccgtg
     2101 ctttccaaca acgacggctg catggtatcc cgttccgccg aaatcaaagc tttgggtatc
     2161 ccgatggggg cacccggatt caaatatgag ggcctggtcc gtcagcacgg cggcaccttg
     2221 ctctcgtcca attacgccct ctatggtgat atgtccgccc gcgtgatgga gaccctgtct
     2281 cacttcgctc cggaagtgga gatttactcg atcgacgagg ccttcctggt gctgaccggc
     2341 ctgcggctca aagacctgga agaatacgga cgcaaaatcc gcaagaccgt cacccaatgg
     2401 actggcatcc ccgtttcggt gggaatctcc acttccaaga ccctggccaa ggtggcaaat
     2461 cactgggcca aacgggttcc cggtttcaaa ggttctctgg cgttgatgga tgaagacagg
     2521 atcgccaaag ccctgcaaaa aacaccagta ggcgaggtgt gggggatagg gcgtcagtat
     2581 gagaagttcc tcatccagaa tgggataaac accgcctggc agctccgcaa cgccaatgag
     2641 aaattcgtgg accactacat gacggtggtg ggacataaga ctgttttgga actgcaaggc
     2701 ttttccgcca tcgagattga cgaagtccca tctcccaaaa agagcatcgt ggtctcccgc
     2761 tcctttggaa aacaggtgga atccctggcc gaactggagg aagccgtctc cacttatatc
     2821 acccgggccg cggaaaaact gcgggtccag gattccgtgg cgggacggat gatggtgttt
     2881 ctgagcacca accgcttcaa agaagggccc caatacagca attccctcca gacggtgctt
     2941 tcacctccca gcgcttatac tccggacctc atcaggatcg ctctggagct gctcagagag
     3001 ctgcatctgc ccggttttga atacaagaaa gcgggggtga tgtttagcga gatcatccca
     3061 tccggagacg tcccgctcag tttcatcgag accaattatc tggacgaccg gcgcaaggtc
     3121 ctgatggaca cagtagacaa aataaaccgc agaagcggca gggatacagt gttttacgcc
     3181 agctccgggg taaaaaagga atggcagatg aaacgcgcca gactctcacc cgcctacacg
     3241 acccgctgga gcgaccttcc gagggccaaa tgagcgactg gcacactctg gaacaagctt
     3301 gtgaagcaaa gatcacgatc catcggtccc gcttcatcgc gttcatagtc ccggtgagcg
     3361 atcccgctca ggcggcaagt gagattgccg tccgcagcca tgaatttgag gacgctaccc
     3421 acaactgcta tgcctgggtc actggtttca agcgggagca gagctattac tccgatgccg
     3481 gtgaacctgc cggcacggct ggcaaaccca tcctcaacgc gctccttagt gccggactca
     3541 ctaacgtggc ggcgaccgta acgcgttatt acggaggcgt aaaattgggc gttcgcggtc
     3601 tcattgaagc ctacagccag agcgtggaag aggctttaaa aactgcctcc ctggtgcccg
     3661 ctatcccgat gtcaactatc tggatcacaa gcgattattc cgatgccgaa agcgcgcaaa
     3721 gactggtcca aaagctgggc ggcggcgttg tggatatccg ttacgaggcc cgggtggaga
     3781 cgattctgag cttgccggag agatcactcg cggagttttg tgaatttctg gatggcaggg
     3841 gccgccagaa cggattagtt tatagactaa aggaacaatg atatgcgtat attaataatt
     3901 gcgatgctgt tgcttgttat tattgggaca ttgactgctc aggacgcgcg gataatctcg
     3961 acccgaaacg gcaagaccgt cagtatcgct cagctggcca aagagctcaa aaagtatgac
     4021 gtggtcttct ttggagaatt ccacgacgac gccactctgc acgccctcca ggccgaattg
     4081 ctccccctca tgcacaaagc gggcaagcgc ctgatcgtct cctttgaaat gtttgaacgc
     4141 gacgtccagc ctgatctgga tgcctatctg gcaggttctg tcactgaaga gcagtttctg
     4201 cagagctcgc ggccctggtc aaactatggc acggactacc gtcccctcct ggagtttgcc
     4261 aggctcaaaa atctcaaagc catcgccgcc aatatcccac gccggttggc aggtcaggtg
     4321 gcccgcaatg gaacagccgc gctcgatgat ctcagcaacg acgataaagt cctgatggcc
     4381 ctggagatat cagctcccga gggcaagtat aaagagagat ttatggccac catgaaggcc
     4441 aacggcatgc acgccagccc caacgacgca aatatgctgg agcgtctgta ttatgcccag
     4501 tgcatgaaag acgacaccat ggccgagagc ctggcccaat atctggaacg ttatccccgc
     4561 caccggattg tccattacaa cggcgatttt cacagccggg aatatctggg aacggtggaa
     4621 cgcctcagaa gccgcgtccc caaggccagg atagctgttc tgagtcctca ttatgccagc
     4681 ctgcctcttc ctgaaacccc gtcagaactg gcagactatc tgattgtcat accagaaaag
     4741 ccagagcaga atcaggagga ttaatcctat gatccgcaaa gccactcatg ccggaaagtt
     4801 ctatccccgt tttggttacg ccatcatcag ccagttggct ggctggacgg ccgagagccg
     4861 caaagctcct cacaccgagc acagcctcgg attgatcgtt ccgcacgccg gctatatgta
     4921 ttccggcgct atcgccgcca gagcttatca tcacatctcg gaagaaggct ttgacgcctt
     4981 tgtggtcttg cacccctccc atcatggcgg caactttgat tacagcgttt cggctttcca
     5041 ggagtatgaa accccccttg gcaacatccg gctcgacacc gaatgctatg ccgctctcac
     5101 cagtggagac gaacccagcg atctctggct gcgctatcat gagcaggagc actccatgga
     5161 gatccagctt cccatgatca agtatttctt tcccgatgcc agaatctgcc ctgtgatgct
     5221 ggggcgccaa aacctcaaca actccagacg cctggcagac cggatctcct atctcctcac
     5281 tcagacttcc agacgcgtgg caattattgc ctccacagac ctttctcatt atcacgatgc
     5341 cgccagcgcc gagaccaaag acaagctgct ggccaagctg gtgcaggaac aggctatcga
     5401 tgatctctgg gacctgatta atgacggcga ctgcgaagcc tgcgggatcg gggccatcct
     5461 cactttgctg tgcttgggtt ccaacgtccc cggcgctaaa atgcaggtgc tggaatacac
     5521 ccactccggc ctggtttccg gccataatca acaggtggtg ggctatctgg ccgccaagct
     5581 gtggcgctaa cctggcgtcg gattcctgcg ttcactacag agatacagag gtttcttttt
     5641 tcactacaga ggcc
//
LOCUS       DAOSFN010000145         5619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_12752_length_5619_cov_9.735802, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000145 DAOSFN010000000
VERSION     DAOSFN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12752_length_5619_cov_9.735802"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..3506
                     /locus_tag="PLO35_08425"
     CDS             288..3506
                     /locus_tag="PLO35_08425"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH79734.1"
                     /translation="MRNTFLIVLLALLGLTTLSANQPMFPKDPSISPDGNDVCFEYDG
                     DLWIVPFWGGSARRLTSTSALEWGPQWSPDGKTIAFNSNREGVAYPYQIATAGGPAVP
                     IIKESYTVVEWFADAQALLAARYNPRFGTSFYRLPLDGSRPTLLAEIGDRFATLSPDN
                     KSIVFNRYGDAYRESYQGSLAGELWKIDLATKEYTRLTNTNHTERYPRFAASSGALFY
                     CESDGERYQLAKVENLDFTQVERLSSLTQWSARDISVARTSERVVFEHFNELWKYDPQ
                     RQHSAQVMKLDIRIPEDQWLDTRRVDSMRNDVWQFCVSDDEKLLGLQYKFDAFLKPRE
                     GGDARRVTYDQAGISGMEFLEDKRTLVLQKLDHGRQKLFTVNASEPTILKELDWFGRD
                     SLNVESFYRDLSGKWVIYYTGPTMSGQVAIADSGLVNFRPLNASGPVTSRVGINSDAS
                     WAAYTCTRADYIRELYVYEFATGSHTKLLNDDSWTSDIIWTNDNRSLILSRGGNISRL
                     DLMPRDEFELEKDYWAEVFAPISTEADSGDIEDEKQSDALSTENSDELAPVEEPELEE
                     TEPQSPVVNETIPEAEPKPPTKIEIWWPELEKRVYPIVTDSEAQLTPYRVKTDSTFYY
                     LSNGGYYGKPTILKSVNLYGKNIKDELNFGMSAWGFVEKNGNFYYVLDGMLRTQWPGG
                     NRKDIRIDLEYEWDLKTLNTRVFEEAWGVFGDNFYDPNMHGKDWQAMYKLYLPYAEKA
                     QNIDQIGTIVDEMIGDLNASHTGFYPRTEQVSVSRSVASLGLEFDQAAVLERGLRISL
                     VYPGTRLSQFFKLQTGDLLTSIDGVVINSHTSIDSLLLDKIGKRVFLELERAGEIIKA
                     DIKGLSFSELRRLEYNYRIDQRRALVKQLTDNQVGYIHIPAMGQRDYENFTRELYRDN
                     LDKKALIIDVRGNVGGRIHDQLLTLLMKKQYATSTSRRLRYQHSPEPFRVWDRPSIVL
                     VDERSFSDGEIFPIIYKELKLGKVVGIPSSGAVIGTWEYELIDGSSMRLPGSGWYKMD
                     GTNMEGTGAMPDIIVENTPNDVISDRDPQLLRAIEEIILELK"
     gene            3562..4440
                     /locus_tag="PLO35_08430"
     CDS             3562..4440
                     /locus_tag="PLO35_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH79735.1"
                     /translation="MTDQSLNNLILRAEALMKTNWLHAVHLLEEAISTHPEDPRALIS
                     LGEFYMRRQMYPQAIAKLQSALQLAANDDLIKYLIGNCFFADGNYRMAVVYYDQIASP
                     WNDILYNKALALAYMGKSQESVVIIRELVKYPDANPFIYFLLVEQLLRNQEYKEALRY
                     VQLAEGKMGKHKHLMLLKALIYSRLEVWIQAYDSFTKADADQGLTNSDYLHSYAVSAM
                     RIGLDSKAIELLHRALEDNPYSSLFYEELLRLLIQQKDMKAARKVLTQAKKHVTRFNP
                     MLKLLQARITNEEGSN"
     gene            4494..>5619
                     /gene="manA"
                     /locus_tag="PLO35_08435"
     CDS             4494..>5619
                     /gene="manA"
                     /locus_tag="PLO35_08435"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOH79736.1"
                     /translation="MQTLTSRPDLMGKPVAELWMGAHPKAPSRIVLGGGEQALDLYLD
                     SHPEALGPAAQVYDGLPFLMKVLAAAKPLSIQAHPNAAQAREGFDRENALEIPLDAPS
                     RNYKDPRHKPELICALTPFTALCGFRPYPQIIAGLRHCGLDQVLPEAQDFIQSASRTA
                     WQSCFQAILAMDASAAVRVVENARGTQEQRLGPGMQDALRWVGKLAELYPGDAGALAP
                     LYLNLVELQPQEALYLDAGIPHAYLNGVGIEVMANSDNVLRGGLTPKYINREELIRVL
                     DFGPLDLKVLKAVPDKTGVGWFHTPAREFSLGIADIMAGQALKTSISGPAILLCQSGN
                     ILISTSGEGYNALKGDAFFIDARATKITLTGEGKAFICVTA"
BASE COUNT         1366 a         1489 c         1517 g         1247 t
ORIGIN      
        1 ggttagagct ttgctggtta gagcttcgct ggttagagct gcgctggtta gagctgcgct
       61 ggtgagagct tcgctggtta gagcttcgct ggttagagct tcgctggtta gagctttgct
      121 ggttagagct acgctggtta gagcctcgtt ggttatatct gcgctgtgag agctgaagct
      181 gtggaactga ttggtcggct gttattcgcc aaaactttaa aactcaaaaa ctctaaaacc
      241 ttaaaaccct acaaccctac aacccaaaaa ctcacacagg agccataatg cgcaatacat
      301 tcttgatagt gttgctggcc ttgctcgggc ttacgacact gagcgccaat caaccgatgt
      361 ttcccaaaga tcccagcatt tcgccggacg gcaacgatgt ctgttttgaa tacgacggag
      421 atctctggat agtgccattt tggggtggat cagcccgacg gctgacatcg acaagcgcgc
      481 ttgaatgggg gcctcaatgg tctcccgacg gcaaaactat agccttcaat tccaaccgag
      541 aaggggtggc ctatccttat cagattgcta ctgccggtgg acctgctgtt cccatcatca
      601 aggaaagcta cacggtggtg gagtggtttg cggatgccca ggcccttctg gcggccagat
      661 acaatcctcg ctttgggacc tctttttatc ggctgcctct ggatggcagc cgtcccactc
      721 tcctggcaga gatcggagat cgctttgcca ccttgtctcc agacaataaa agcattgttt
      781 tcaaccgcta cggcgatgct taccgggagt cataccaggg cagcctggcc ggcgagctct
      841 ggaagattga tctggccact aaagaatata cccggcttac aaacacaaac cacacagagc
      901 gttatcctcg atttgcggct tccagcggag cactcttcta ctgcgaatcg gatggagaga
      961 ggtatcagct ggccaaagtg gagaatctgg attttaccca agtggagcgc ctcagcagcc
     1021 tcacgcagtg gtcagccaga gacatcagcg tggcaaggac cagtgagcgg gtggtgtttg
     1081 aacactttaa cgagctctgg aaatacgatc ctcagcgtca gcacagcgct caggtgatga
     1141 aactggacat ccgcatcccg gaagaccaat ggctggacac ccgccgcgtc gatagcatgc
     1201 gcaacgacgt atggcagttt tgtgtgtcgg acgatgagaa gctgctgggc ctgcaatata
     1261 agtttgacgc gtttctgaag ccccgcgagg gtggagatgc caggcgggtc acttatgacc
     1321 aggctgggat cagcggtatg gagtttttgg aggataaacg cactctggtg ctgcagaaac
     1381 tcgaccatgg ccggcaaaaa ctattcaccg tcaacgccag cgagcccacc atcctgaagg
     1441 agctcgattg gtttggccgc gacagcctca acgtcgagag cttttatcgt gacttaagcg
     1501 gcaaatgggt gatctattat acgggaccga ccatgagcgg acaggtggcc attgccgatt
     1561 ccggattggt gaatttccgt ccgctcaatg cctccgggcc ggtgacatcc agagtgggga
     1621 tcaactccga tgccagctgg gcagcctata cctgtacccg ggctgattat atccgggaac
     1681 tctatgtgta tgagtttgcc actggcagcc ataccaagct gctcaatgac gacagctgga
     1741 cctccgacat catctggaca aatgacaacc gctctcttat cctctcccgc ggtggcaata
     1801 tctccaggct ggatttgatg cccagagatg agtttgagct tgaaaaggac tattgggctg
     1861 aggtgtttgc tcccatcagt actgaggcag acagcggcga tattgaagac gagaaacaga
     1921 gcgacgcgct ttcaactgaa aacagcgacg aacttgctcc tgtggaagag cctgaactcg
     1981 aagagactga gccccagagc cctgttgtaa acgagactat tccagaagct gagcccaagc
     2041 ctcccaccaa gatagagatt tggtggccgg agctggaaaa acgcgtctat ccgatcgtta
     2101 cggattccga agcacagctc actccctatc gcgtcaagac ggactccacc ttctattatc
     2161 tctccaatgg cggttattat ggcaaaccca ccatcctcaa atccgtcaac ctctatggca
     2221 aaaacatcaa ggacgagctg aattttggca tgagtgcctg gggttttgtt gagaagaacg
     2281 gcaacttcta ttatgtgctg gacggcatgc tgaggacgca atggccaggt ggaaaccgta
     2341 aggacatccg gatcgatctg gaatatgaat gggacctcaa gacgcttaac acccgcgtct
     2401 ttgaagaggc ctggggtgtg tttggcgaca acttttacga tcccaacatg cacggaaaag
     2461 actggcaggc catgtataag ctctatctgc cctacgcgga aaaagcccaa aacatagatc
     2521 agattggcac catcgtcgat gaaatgatcg gtgatctcaa tgcctcgcac acgggttttt
     2581 atccccgcac ggaacaggtg tcggtcagcc ggtcggtggc cagtctgggc ctggaatttg
     2641 atcaggcagc ggtactggaa agaggcttga gaatctctct ggtctatccc ggcaccaggt
     2701 tgagccagtt cttcaagttg caaacgggag atttgctcac ctccatagat ggagtggtga
     2761 tcaattccca cacttccatc gattccctgt tgttggacaa gatcggcaag cgcgtctttc
     2821 tggaactgga aagagcgggt gagatcatca aggccgatat caagggcttg tcattttcag
     2881 agttacgccg tctggaatac aactatcgca tcgatcagcg acgcgcactg gtcaagcaat
     2941 tgacagacaa tcaggtgggc tatatccaca ttcccgccat gggacagcgg gactatgaaa
     3001 acttcacccg cgagctctat cgcgacaatc tggataaaaa agccctcatc atcgatgtgc
     3061 gtggcaatgt gggcggcagg attcacgatc aactgctcac cctcctgatg aaaaagcaat
     3121 atgccacttc cacttccaga cgcctcagat atcagcactc tcccgaaccc ttcagagttt
     3181 gggatcgtcc cagtatcgtg ttggtggatg aaaggtcctt ttccgatggt gaaatctttc
     3241 cgattatcta caaagaactc aagctgggca aggtggtggg catcccctcc agcggagcag
     3301 tgatcggcac ctgggaatat gagctaatcg atggctccag catgcgttta cccggttccg
     3361 gctggtataa aatggacggc accaacatgg aaggcaccgg agccatgcca gatattatcg
     3421 tggagaatac tcccaacgat gtaatctccg atcgggaccc tcaactgctg agggcaattg
     3481 aagagatcat cctcgaactg aagtaagagc aaaaatcctc cccagccctg gattctgggc
     3541 tggggaggag tggaaaacat aatgacagac caaagcttaa acaatctgat cctaagggcg
     3601 gaagccctga tgaagaccaa ctggctgcac gcggtccatc tgcttgagga agctatctcc
     3661 actcatccgg aggaccctcg cgccctcatc agcctgggtg aattctatat gcgccgccag
     3721 atgtatcctc aggctatcgc caaactgcag tccgccctcc agctcgcggc caatgatgat
     3781 ctgatcaaat acctgatcgg caactgcttt tttgcagatg gcaactaccg catggccgta
     3841 gtctactatg accagattgc cagcccctgg aacgatattc tctacaacaa agctctggcg
     3901 ctggcctaca tgggcaaatc ccaggaatcg gtggtgatca tccgcgaact ggtcaagtat
     3961 cctgatgcca atcctttcat atatttcctg ctggttgagc aactgctgag gaaccaggag
     4021 tataaggaag ctctgcgtta tgtgcagctt gcagaaggca aaatgggcaa acacaagcat
     4081 ttgatgctgc tgaaagctct gatctattcc cggttggaag tgtggatcca ggcctacgac
     4141 tccttcacta aggccgatgc cgaccaggga ctgacaaatt cagactacct gcacagctac
     4201 gccgtcagcg cgatgcggat tggcctggat tccaaagcga tagaactgct ccaccgggct
     4261 ttggaggata acccctactc cagcttgttc tacgaagagc tcctgaggct gctaatccag
     4321 cagaaggata tgaaagcggc ccgtaaggtt ctgactcagg ccaaaaagca cgttacccgc
     4381 ttcaatccca tgctcaaact gctgcaggcc aggatcacca atgaagaggg gtccaactga
     4441 tcaacagact gctcaacagc tgtcaggaat atgactgggg ttccggagag tatatccaga
     4501 cgctgaccag ccgtccggac ctgatgggca aacctgtggc cgagttgtgg atgggagcgc
     4561 atcccaaagc gccctccagg atagttctgg gtggtgggga gcaggccctg gacctttatc
     4621 tggacagcca tcccgaagct ttgggcccgg ctgctcaagt ctatgatggt ctgccatttc
     4681 tgatgaaagt actggccgcg gctaagcctt tgtccattca ggcgcatccc aacgcagccc
     4741 aggcccggga aggatttgac cgggaaaacg ctctggaaat tccgctcgac gccccttccc
     4801 gcaactacaa agacccgcgc cacaaaccgg aactgatctg cgccctcact ccttttactg
     4861 ccctttgcgg ttttcgtcct tacccgcaaa tcattgccgg gcttaggcat tgcgggctgg
     4921 atcaggtgct gccggaggcc caggacttta ttcaatctgc ttccaggacc gcctggcagt
     4981 cctgttttca ggcaatactg gccatggacg cttcggctgc tgttcgggta gttgaaaacg
     5041 cgcgcgggac gcaggagcaa agattgggac cggggatgca agacgcattg cgctgggtgg
     5101 gaaaactggc agagctgtat ccaggggacg cgggagcgct ggctcctctg tatctgaacc
     5161 tggtggaact gcaaccccag gaagccttgt atctggatgc cggaatccct cacgcctatc
     5221 tgaacggcgt cgggattgaa gtgatggcca attccgacaa cgtgctcaga ggagggctca
     5281 cccccaaata catcaacagg gaagagctga tccgggtgtt ggatttcgga cctctggacc
     5341 tgaaggtgct gaaagctgta ccagataaaa cgggtgtagg ctggtttcat acgccggccc
     5401 gggaattctc tcttgggata gctgatatca tggcaggcca ggccctgaaa accagtatct
     5461 caggccccgc gattcttcta tgtcagtctg gaaatatcct gatttcaacc agcggggaag
     5521 ggtataacgc tctgaaaggc gacgcgtttt tcatcgatgc ccgggcaacc aagataaccc
     5581 tgacaggaga gggcaaggct tttatctgtg tgacagcgc
//
LOCUS       DAOSFN010000146         5597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_12822_length_5597_cov_7.191808, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000146 DAOSFN010000000
VERSION     DAOSFN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12822_length_5597_cov_7.191808"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            772..5592
                     /locus_tag="PLO35_08440"
     CDS             772..5592
                     /locus_tag="PLO35_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79737.1"
                     /translation="MKRVLIFFACMSVLTLGAIRVSNHPVSHIQPNGVKVDLLLNGDE
                     FYHYLTDSKGYTVLIDPGSDAAVYAGQKDGKLVTSELVVGINDPAASGLEPGFQPSQE
                     EIRRQVMESGGENGSRDGNAPSTGTINNVVIFVRFLDQAEYTQPISTYDTAFNSSTTA
                     SLRGFFLQESASQLTVNSTFYPAAVGGIVRSFQDGHARGYFSPWTASNPIGYDGISEG
                     KHRLWLMMQYAVNSVAASIPGSIDLDADNDGSVDNVTFICQGTFDTHADVLWPHHSYL
                     FPYTLVQIGSLWVTDYNLQLSDFIHPGVLSHEYSHSMNFPDLYHYDNNLDPVGAWDPM
                     ASTSNPPQHHTSYLKHKYGGWFASIPLITPTSTATQYSLTAIDQNPFACYRIASTRPN
                     EYYVMEYRRDTGTYESGVPASGLIVYRVLSSYGGSALWGNPHGPPDEVYVYRPGINAN
                     NENGYIDAANFSLQSGRVDIYTGTDPSPWLYGDGSAELPGNLVITNIGSTGGTSISFW
                     IRDSSPNRWLGISGNLWTQPINWSHGTVPTVDDDVVVPETSGWDPLINIPGQACKSLE
                     LRSGAELTIAAGSLEVADNLTSYGTIDLTSSDATLYVYDDLNLMSGSLTRFYNAGAEI
                     VVEEDLRFASGATANWSQGYLEFSGSGTSSLICESPLSIANLRIEKTGGGACVIPATN
                     TAALTVNGAVTVQSGTLSHGYTGTTIVKGNLHAYSGSLNFYAGTVSLEGNSSSSIRID
                     NSGSYLHKLRIAKSGSAGVSLSTSNTVVGNDLSIVSGYLSTGANTLYVAGNWVNGVGP
                     DAFYEGTGRVVFNGLSNQNCSSETFNILELNKSGRLLVPTGSTISCAVYDWTSGGVSV
                     SGGSFSASDLADDGIFGSYTLSAGSIELTQDASHYVDLRSANINISGGVFSVYGGGGA
                     MWMAYQGTTTFTMSGGYFDVRDQDILISSAYLLNETITGGYIRTRGDLTVQRTDFNPA
                     GGYLEFWGGTNATVFVAAGSTLSTLVVNKTSTREDQDFRLSRDGTRTTLTRANTLSAS
                     GNLVISDVLIILNGVFDVNGQTITVNNDLTVNGTLKMSSGSLTVNDTVTWNGSAQVSG
                     GSISCAGEWTFGSSSTALLNGSQVLINNPYGGVLTNHSSLAGFGNLEINATEEDPVCH
                     YSSPAGAPLKVAGTLLLNGSVTLNLNSFGCQAQSLSINEDCALLVGDGGSLDIPSSLT
                     LAGRLDVGPGTANLHGFMNFPSTGLLQIAWGSFVCDAPWFDMRGTVYLRGGMDIDNGS
                     FEIRNNHVYLAAHPARLFSNAMLNFGRNFTAIEPGAYEPIWGSLIMNGTASASLNVSS
                     PNFLPATDIQKEGEAVVSLAADTTLQGLLSVYSGTLDLAGYTMNALADLQVQGVLLLS
                     PASQLHIEGGRTLSMFSGGQLHALGLDSSPVGISHYGSTGYYDIDAMDNSFVKAAYCV
                     FEHMGPNGIYLHEGALVDQTTAFEHCIFQSGAPGGWLLRMDNNQTLTVNGPLFPANTW
                     SGAGNVLKTVNSGSTTFIAYSGAFSGPTYETDPFNRINWQPGGIMPVQNLRISATEDN
                     YTLNWDYSYPYSCFKVYSADSPDGTFSFLGTSSALNWTAQITGARRFYRVTAVQ"
BASE COUNT         1249 a         1688 c         1496 g         1164 t
ORIGIN      
        1 acctctctgc agccattata agctgaagag cgttgggagg caagcggaaa aagcgggccg
       61 cgcggttgta atacagacgt tatgtcagcc gtgaactact ggccggtatg aagaaaaggg
      121 gatagattcc aggctgggac agctgttcag tccgtcctca ccaacaggcc tcgtttcagg
      181 atggtactca aagtcagctt ccattcttcc cagacggttt cgtcatgaaa gtcccgctcg
      241 ttgatcatag agatcagggg agcgaggtag atcgcgctgt atcctatggc caggaggcaa
      301 tcttcgccgg gctctcaact cctgcccgct gaagggaggc catatcagcg gaagctgttt
      361 gggcagagag agttaattat ccagggtgag atgtccgcca tcctagtgat tggcattaga
      421 taagagtcgg ttgcacgtaa tggatcacaa gctctactgc cggtcttcgc acctctctga
      481 agccatgatc cctattgtgc tgtttttaat agattaaccc atgcgatcaa ccagatgatt
      541 tttatcactg atcagccggg gagcgaatat gaaacgaaag caaagaccat gttcaaagca
      601 gctgaacgca caagtacacg ttgttggact ctccgctggc gatcatcagc tattcagccg
      661 cagatattgc ttgacaaaag caaagccaca atctattgat cccaaaaatg ccagagccgg
      721 aagcttgcca accggcacta tgttacggac tatgcttcca ggggagtcaa gatgaaacgt
      781 gtgctgatat tttttgcctg tatgagcgtg cttacccttg gcgcgatcag agtatccaat
      841 caccctgtca gccatatcca gcccaatggg gtcaaggtgg acctcctgct caatggagac
      901 gaattttatc actatctcac ggacagcaag ggctataccg tcctgatcga tcccggcagt
      961 gacgcggctg tctatgcagg gcagaaggat ggcaagctgg tcacttcgga gctggtggtg
     1021 gggattaatg accccgcagc ttcaggcttg gagccagggt tccaacccag ccaggaggag
     1081 atccgaagac aggtgatgga aagcggcggt gaaaatggca gccgcgatgg caatgctccc
     1141 agcaccggca ccatcaacaa cgtcgtgatc ttcgtgcgct ttttggacca ggcggaatac
     1201 acccagccca tttccaccta tgacaccgcg ttcaacagct ccaccacggc ttctctgcgg
     1261 ggctttttcc tgcaggaaag cgccagtcag ctcacggtga acagcacttt ctatccagcc
     1321 gcggtgggag gaattgtgcg cagctttcag gatggccacg cgcggggata tttctccccc
     1381 tggactgcct ccaaccccat cggctatgat ggtatttcag agggaaagca ccgtttgtgg
     1441 ctgatgatgc aatatgccgt aaacagcgtc gctgcaagca ttcccggctc catcgatctg
     1501 gacgcggaca acgacggcag cgtggataac gtcaccttta tctgtcaggg cacttttgac
     1561 acccatgccg atgtgttgtg gccccatcac agctacctgt ttccctacac gttggtgcaa
     1621 attggctctt tgtgggtaac cgattacaac ctgcagcttt cagattttat ccatccaggc
     1681 gtgctttctc acgagtattc ccactccatg aatttccctg atctctatca ttacgataat
     1741 aacctcgacc ccgtcggagc ctgggatccg atggcatcca cctccaatcc gccccagcac
     1801 cataccagct acttgaaaca caaatatggc ggttggtttg cctccatacc tctgatcact
     1861 cccacttcca cggccactca atacagcctc acggccatag accagaatcc ctttgcctgc
     1921 taccggatcg cctccacccg tcccaatgaa tactacgtga tggaatatag gagagacact
     1981 ggtacttatg aatccggggt tccggcttcc ggattgatcg tttaccgcgt cctcagcagc
     2041 tatggcggtt cggctctttg gggcaatccg catggtccgc ccgacgaggt ctatgtctat
     2101 cgtcccggca tcaatgccaa caatgaaaac ggctacatag acgcggccaa cttttcgctc
     2161 cagagcggcc gggtggatat ctacaccggc accgatccct ccccctggct gtatggcgac
     2221 ggttccgcgg aactgcctgg caacctggta attacgaata ttggatcgac tggcgggacc
     2281 agcatcagct tctggatcag agacagctct cccaaccgct ggctgggaat atccggaaac
     2341 ctctggaccc agcccatcaa ctggagccac ggcaccgttc cgaccgttga cgacgacgtc
     2401 gtggtgcctg aaacaagcgg ctgggaccct ctaatcaaca ttcccggtca ggcatgcaaa
     2461 agcctggaac ttaggtccgg ggccgagctc accattgccg ccggcagtct ggaagtggcg
     2521 gataacctga ccagctatgg aaccatcgat ctcacttcca gcgatgccac gctctatgtg
     2581 tatgatgatc tgaaccttat gagcggctcg ctgaccaggt tttacaatgc cggggccgag
     2641 atcgtggtgg aggaggacct ccgttttgcc agcggggcta cagccaactg gagccagggc
     2701 tatctggaat tcagcggatc gggaacctcc agcctcatct gcgagagtcc cctgagcatc
     2761 gccaatctca gaatcgaaaa gacgggtgga ggcgcttgcg tgatcccagc caccaatacc
     2821 gcggccctga ctgtcaacgg tgcggtcacg gtgcaatccg gcactctaag ccacggctac
     2881 acaggcacta ccatcgtgaa gggcaacctt catgcctata gcggcagcct caatttctat
     2941 gcgggtaccg tcagcctgga gggcaacagc agttccagta tccggatcga caactccggc
     3001 agctatctgc acaagctcag aatcgctaaa agcggtagtg ccggagtgag ccttagcact
     3061 tccaacaccg tggtgggcaa cgatctgagc atcgtgagcg gctacctcag cacgggagcc
     3121 aacactctct atgtagcggg aaactgggtc aacggcgtgg gcccggacgc cttttacgaa
     3181 ggcacgggcc gggtggtctt caacggcctc agcaaccaga actgctccag cgagaccttc
     3241 aacatcctgg agctcaacaa gagcggcaga ctgctcgtcc ccaccggttc cactatctcc
     3301 tgcgcggtct atgactggac atccggaggc gtgagcgtga gcggaggcag cttctcagcc
     3361 agtgaccttg ccgatgacgg catctttggc agctacaccc tcagcgcggg gtccatcgag
     3421 ctcacccagg atgcctccca ttatgtggac ctccggagcg ccaatatcaa catcagcggg
     3481 ggagttttca gcgtctatgg aggcggcggg gccatgtgga tggcctatca gggcactact
     3541 actttcacca tgagcggagg atactttgac gtccgcgatc aggatatcct gatcagcagc
     3601 gcctacctcc tcaacgaaac catcacggga ggctatatcc gcacccgggg agacctcacc
     3661 gttcagagga cggatttcaa ccccgcgggt ggctatctcg aattttgggg cggcaccaac
     3721 gccaccgtgt ttgtggccgc gggcagcacg ctcagcactt tggtggtcaa taagaccagc
     3781 acccgcgagg accaggattt caggctcagc cgcgatggca ccaggaccac tctcacccgc
     3841 gccaatactc tctcagcctc aggaaacctc gtcatcagcg acgtcctcat catcctcaac
     3901 ggcgtcttcg acgtcaacgg ccagactatc accgtcaaca atgatctcac ggtgaacggc
     3961 accctcaaga tgagctccgg cagcctcaca gttaatgaca ccgtgacctg gaacggcagc
     4021 gcccaagtga gcggtggcag catctcctgc gcaggagaat ggacctttgg cagttccagc
     4081 acggccctct tgaacggctc ccaggtgctg atcaacaacc cctacggcgg cgtgctgacc
     4141 aaccattcca gcctggcggg ctttggcaac ctggagatca acgccacgga ggaagatcca
     4201 gtctgccact attccagccc cgcgggagct cctctcaaag tggcaggaac cctgctcctg
     4261 aacggttccg tgaccctcaa cctcaattcc tttggctgcc aagctcagag cctcagcatc
     4321 aatgaagact gtgctctgct ggttggagac ggcggcagcc tggatatccc gagctcgctg
     4381 accctggcag gacgtctgga tgtggggccc ggaacggcca acctccacgg cttcatgaac
     4441 ttcccctcca ccggactgct ccagattgcc tggggcagct ttgtctgcga cgccccctgg
     4501 tttgacatgc gcggcaccgt ctatctgcgc ggcgggatgg atatcgacaa cggctccttt
     4561 gaaataagga acaatcacgt ctatctggcc gctcatcccg cccggctgtt cagcaacgcc
     4621 atgctgaact ttggccgcaa tttcaccgcc attgagcccg gtgcctatga gcccatctgg
     4681 ggttccctga tcatgaacgg cacggcttcc gcctctctaa acgtgagcag ccccaacttc
     4741 ctgcccgcca ccgatatcca gaaagaaggt gaggcggtag tatccctggc agcggacacc
     4801 actctgcagg gactgctgag cgtctattcc ggcactctgg acctggctgg ctacactatg
     4861 aacgcgctgg ctgacctcca ggtgcaggga gtgctgttgc tgagtcccgc ttcccaattg
     4921 cacatcgagg gaggccgcac tctcagtatg ttctccggcg gccagcttca cgcattgggg
     4981 ctggattcca gcccagtggg catcagccat tacggcagca ccgggtacta tgatatcgac
     5041 gccatggata acagcttcgt gaaggccgct tactgcgtgt ttgaacacat gggcccgaac
     5101 gggatctatc ttcacgaggg cgctttagtg gaccagacca ccgctttcga acactgcatc
     5161 ttccagtctg gcgctccggg aggatggctg ctcaggatgg acaacaacca gacgctcacg
     5221 gtgaacggac ccctctttcc agccaatacc tggtcaggag caggaaacgt gctgaagacc
     5281 gtcaacagcg gcagcaccac atttattgcc tacagcggtg ctttcagcgg cccgacttat
     5341 gagaccgatc ccttcaaccg cataaactgg cagccgggag gcatcatgcc ggtgcaaaac
     5401 ctcaggatca gcgctacgga agacaactac accctgaact gggattactc ctacccctac
     5461 agctgtttca aagtctatag cgctgacagt cctgatggca ccttcagctt ccttggcacc
     5521 agttccgccc tcaactggac ggctcagatc accggggcgc gacgctttta cagagtgacg
     5581 gcggtgcagt agcgcgt
//
LOCUS       DAOSFN010000147         5592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_12837_length_5592_cov_8.745349, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000147 DAOSFN010000000
VERSION     DAOSFN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12837_length_5592_cov_8.745349"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1255
                     /locus_tag="PLO35_08445"
     CDS             164..1255
                     /locus_tag="PLO35_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79738.1"
                     /translation="MTAYDIKTYTLAELRELYDDPRWWHRTNLPFSKARLGAYLENPN
                     AAETDVIWLIAWRGDAIVAYLGLVPDRINSDTGIRKIAWFSSWWADPTVRGRGLAEQL
                     TSMGLKLYPGIGIDSGTPWSMRKMRESGDFYLCSQRPRSLWMLNVNRRALKDFGMKNA
                     LTDFLFPLAHSCLGFAIRLRLSLWLKRRGERDLSLEYLTAIDQEAMELLLRFRETELC
                     VHDHDTIHWRASLLPKLAHLSGIDSKQSSYFGHSGFRVSSYLAKLWKGSKLIGLVNIC
                     LADGYLKIPFLYIEDNCERELSVLIARLCLEESIDVVYSQDRRVKTALERFKLPRWLL
                     KSYPMDVLLSNSLMDVPADKILQDGDGAF"
     gene            1252..2202
                     /locus_tag="PLO35_08450"
     CDS             1252..2202
                     /locus_tag="PLO35_08450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOH79739.1"
                     /translation="MSYRFKLAGLDLKLSNNRGHRILYFHQVTLENPPYYDGGISPER
                     FLELLFAVRKLGFRFIPLEEALSSQTALRGTVSLCSDDGLACNYHSVLPILEDLGIGL
                     CVFLIGKCIDNKDFAWNHLLMLLRSQVSDTGLQAVLPEIQSKFSLHDTGLISSTLFSV
                     AQERRDELIAYLWERFGLSDPYDILHKEQPFLSSAQLSEMSLKGVTLALHSHTHADLS
                     RLGFKDLKQELLLNIEALEQRSLPWQPWLAFPYGRQCSMEHMRKLRQELGIERFFGIR
                     FQSGDNRPEDLLWQRIGMETDGFTTWRDLLLKPFVRRLKG"
     gene            2536..3849
                     /locus_tag="PLO35_08455"
     CDS             2536..3849
                     /locus_tag="PLO35_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOH79740.1"
                     /translation="MQRRVIIMGAAGRDFHNFNVFFRDNAEYKVVAFTATQIPGIDDK
                     KYPAKLAGKLYPKGIDIHPEAELKDLIKKHKVDLVVLAYSDLPHEVVMNKASLVNAAG
                     ADFMLMGAKNTMVKTTKPLVTICAVRTGCGKSQTTRAVINALKDKGLKVVSIRHPMPY
                     GDLVAQKVQRFAKLSDLKKHKCTIEEMEEYEPHIQMNSVIYAGVDYEAIVREAEKEAD
                     VIIWDGGNNDIPFYCSDKQLNIVVVDPHRPGDEISYYPGETNVHMADVIVINKIDSAD
                     LDDINEVRANVRMINPKAQIIDAASPLSVDKPELITDKRVLVVEDGPTLTHGEMTYGA
                     GVIAAEKYGCAELVDPREWAVGEIAQTFAKYPDIGVLLPAMGYSDQQIKDLEQTINST
                     DCDAVVIATPIDLRRLIKIKKPACQVQYDLQEIGRPTIADVLKNF"
     gene            4018..4968
                     /gene="arcC"
                     /locus_tag="PLO35_08460"
     CDS             4018..4968
                     /gene="arcC"
                     /locus_tag="PLO35_08460"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOH79741.1"
                     /translation="MNKTAVLALGGNAIIKAGEKGTIGQQFANTRDSLGGVVELISQG
                     YKLAITHGNGPQVGNLLRQQEAGEKEGIAPLPLGVLNAATEGTMGYMIEQSLQNKLLL
                     SGIDKQVITIISQVVVDKNDPSMLNPTKYVGSTYYSEEQASQLKQELGWTLREDAGKG
                     WRRVVPSPYPTRIIPAATIKELVERGEIVIAVGGGGIPIYIEENGTYEGVDAVIDKDF
                     ASMLLALNIEAGLFVILTGVDKVAVDYGKPTQRDLDRVTVSEARRYYEEGQFPAGSMG
                     PKILAAIDFLSRGGSEVLITSIDRIVDAFAGKTGTRIVKD"
BASE COUNT         1440 a         1550 c         1416 g         1186 t
ORIGIN      
        1 gctcactagt ccctgaaact agcgtgtata ccaatttcac cacttcagca cgttgcgttt
       61 ggtactctct ttttgagggg cgattattgt caagagcttt ttttccgctt gacagcagcc
      121 atgcctctga ttcatgggcg taaaacacat tagggacgtg agcttgaccg catacgacat
      181 caaaacctac acgctggcag agcttagaga gctttacgat gatccgcgtt ggtggcatcg
      241 caccaatctg cccttcagca aagcgcgcct gggggcctat ctggaaaatc ccaacgctgc
      301 cgagactgat gtcatctggc tgatcgcctg gaggggagac gcgatcgtgg cttatcttgg
      361 cctggtgcca gaccggatca attcagatac cggcatccgc aagatcgcct ggttcagttc
      421 ctggtgggca gatcccacgg tgaggggcag aggtttagcg gaacagctca cgagcatggg
      481 tttgaagctg tatcctggca tcggaatcga ttccggaact ccctggtcca tgcgcaagat
      541 gcgtgaaagc ggagactttt atctctgttc tcaaaggccc cgcagcctct ggatgctcaa
      601 tgtcaaccgc cgcgcgctca aagactttgg catgaagaac gcgctcacgg attttctgtt
      661 tcccctcgcg cacagctgtc tgggtttcgc gatcaggctg aggctgagct tatggctaaa
      721 gcgaaggggg gagagagatc tgagcctgga ataccttacc gcaatcgatc aagaggccat
      781 ggaactgctg ttacgattca gagagacaga actctgcgtt cacgatcatg atacgatcca
      841 ctggcgcgcc tccctgctac ccaaactggc ccatctgagc ggaattgact caaaacaaag
      901 cagctacttt ggtcacagcg gctttcgggt gagcagctat ctggccaaac tctggaaagg
      961 gagcaagctg atcggccttg tcaatatctg cctcgcggac ggctatctca agattccatt
     1021 tctatatata gaagacaatt gtgagcgcga actcagcgtc ctgatcgcca ggctatgtct
     1081 ggaagagagc atagatgtcg tttactcaca ggacaggagg gtcaagactg cgctggagcg
     1141 cttcaagctt ccccgctggc tgttgaaaag ctatccgatg gatgtcttgc tctcaaacag
     1201 ccttatggac gttccggctg ataaaatcct ccaggatgga gacggagcat tttgagctat
     1261 cgcttcaaac ttgcgggatt agacctgaaa ctgtcgaaca accggggcca caggatcctc
     1321 tattttcatc aggttaccct tgaaaatcca ccctactacg atggtgggat cagccccgaa
     1381 cgctttctgg aacttctctt cgcggtgcga aagctgggat tcagattcat ccccctcgag
     1441 gaagctctat cctcccaaac tgctctacga ggaaccgtga gtctctgcag tgacgatggc
     1501 ttggcctgca attaccacag cgtgttgccg atcctggagg acctggggat tggactgtgt
     1561 gtctttctga taggtaaatg tatagacaac aaagatttcg catggaatca cctgctcatg
     1621 ctgctcaggt cgcaagtaag cgacacggga ctgcaagccg tcctgccaga gatacaatcc
     1681 aagttttcac tccatgatac gggcctgatc agctccaccc tgttcagcgt agcgcaggaa
     1741 agacgggatg aactgatcgc atatctctgg gagcggttcg gactctcaga cccttatgac
     1801 atactacaca aagaacaacc tttcctcagc agcgctcagt tgagcgagat gagcttaaaa
     1861 ggtgtgaccc tggccctgca cagccacact cacgcggacc tgtcacggct aggttttaaa
     1921 gacctcaaac aagagctgct tctcaacatc gaagctttgg aacagcgtag tctgccatgg
     1981 cagccctggc tggcctttcc atacggcaga cagtgttcca tggagcatat gcgaaagctg
     2041 cgccaggagt tgggtattga acgctttttt gggatccgat ttcagagtgg cgacaaccgt
     2101 ccggaagatc tcctctggca acgtatcggc atggaaacag atgggtttac gacctggcgg
     2161 gacctgctgc tgaagccgtt cgttagacgc ctgaaaggat agagcgcagg tgtttttccc
     2221 gcagattacg cagattggta cgctgatcac gcagattttg gcgaggttaa cagagtttat
     2281 caagttgaca aggttgagga ggtcgaggag gttgagaagg caggtggtca aatcactcta
     2341 aaccatctaa actatttaca ctatctaaac cgtaaatcag cgaaatctgc gtaccaatct
     2401 gcgtaatccg cgggaagcat tcctgccttc tcaaccttcc aaacctactc aaccgcattt
     2461 ttatccttga ctctattagc ccccctttaa aaatgcctca aaatcataaa tcataataga
     2521 taaaggagta atctaatgca acgcagagtg atcatcatgg gtgccgcagg gcgcgacttc
     2581 cacaacttca acgtcttttt cagagacaac gccgaataca aggtcgtggc cttcactgct
     2641 acccagatcc ccggaattga tgacaagaaa taccccgcca agttggccgg aaagctctac
     2701 cccaagggca tcgacatcca tcctgaagct gagctgaaag acctgatcaa aaagcacaaa
     2761 gttgatctgg tggtgctggc ttacagcgat ctgcctcacg aagtggtgat gaacaaggcc
     2821 tccctggtca acgccgcggg agccgatttc atgctcatgg gcgccaaaaa caccatggtc
     2881 aagaccacca aaccgttggt cactatctgt gccgttcgca ctggttgcgg aaaatcccag
     2941 accacccgtg cggtcatcaa cgccctcaaa gacaaggggc tcaaggtagt ctccatccgt
     3001 caccccatgc cttatggcga cctcgtggcc caaaaagtgc agcgctttgc caagctgagc
     3061 gacctcaaga aacacaaatg caccatcgaa gagatggaag aatacgaacc ccacatccag
     3121 atgaacagcg tgatctacgc cggcgtggat tatgaagcca tcgtccgcga agccgaaaaa
     3181 gaagccgacg tcatcatctg ggacggcggc aacaacgaca tccccttcta ctgctcagac
     3241 aagcagctca acatcgtggt ggtggacccc catcgtcccg gtgacgaaat ctcttattac
     3301 cccggcgaga ccaatgtcca tatggcagac gtgatcgtga tcaacaaaat cgacagcgcc
     3361 gacctcgacg acatcaacga agtgcgtgcc aacgtccgca tgatcaaccc caaggcccag
     3421 atcatcgacg ccgcctcgcc gctttcagtg gataagcctg aactgatcac cgacaagcgc
     3481 gtgctcgtcg tggaagacgg tcccaccctc acacacggag aaatgaccta cggtgccggt
     3541 gtcatcgccg ccgagaaata cggctgtgcc gagctggtcg atccgcgtga atgggccgtg
     3601 ggcgaaatcg cccagacctt cgcaaagtat cccgacatcg gtgtcttgct tcccgccatg
     3661 ggttacagcg atcaacagat caaagacctg gaacagacca tcaacagcac cgattgcgat
     3721 gccgtggtga tcgccacacc gatcgacctg cgccgcctca tcaagatcaa gaagcccgcc
     3781 tgccaggtcc agtatgacct ccaggagatt ggccgtccca ccatcgccga cgtcctcaaa
     3841 aacttctaaa cataattcca gttatcagcc aaggggcaga acctgacaag attctgcccc
     3901 ataacttttc acaccgtgtt ccagtagttg agatgcttcg catgggagag cctgcggccg
     3961 ttaggctatc tttacaacgc aaaaccctaa aactctaaaa ccttataaga ggaatatatg
     4021 aataagacag ccgttctcgc tttgggaggc aatgccatca tcaaagccgg tgaaaaagga
     4081 actatcggtc agcagtttgc caacaccaga gattctctgg gcggggtggt ggaactgatc
     4141 agccagggtt acaaactggc catcacccac ggcaacggac ctcaggtggg aaacctgctg
     4201 cgtcagcagg aagccggtga aaaggaaggc attgcgccac tgcctttggg agtcctcaat
     4261 gcggccacgg aaggcacgat gggctatatg atcgagcaaa gcctgcaaaa taaactcctg
     4321 ctcagcggca tcgacaaaca ggtgatcacc atcatttctc aggtggtggt ggataaaaac
     4381 gatcccagca tgctgaatcc caccaaatat gtgggcagca cctattacag cgaagaacaa
     4441 gccagccaac tcaaacagga actgggctgg actctcagag aggatgccgg aaaaggttgg
     4501 cgccgcgtgg tcccgtctcc atatcccacc cgcatcattc cagccgcaac catcaaagaa
     4561 ctggtggaga gaggcgagat cgtgatcgcc gtaggcggtg gcggaatccc tatctatata
     4621 gaagagaacg gcacttatga aggcgttgat gccgtgatcg acaaagactt tgcctccatg
     4681 ctcctggcgc tcaacatcga ggccgggctc ttcgtgattc tcaccggagt ggacaaggtg
     4741 gccgtggatt acggcaaacc cactcagcgc gacctcgatc gcgtcaccgt gagcgaagcc
     4801 cgccgatatt atgaagaagg ccagtttccc gccggcagca tggggcccaa gatattggcc
     4861 gccatcgatt tcctctcccg aggcggcagc gaagtcctca tcacttccat agacaggatc
     4921 gtggacgcct ttgccggcaa gaccggtacc cgcatcgtga aagactgata agcgccagcg
     4981 aacgctatac ttgaggaatc ggacagcggt tcctcttttt tttatttttc caacctcctc
     5041 ccttattcat ggtccctgtg tctgtctcta ccatctccta cccatcccga actttattgg
     5101 ggatgccttg ggggagataa ggacgggctc ccgatccatg aataagggcc aagagacaaa
     5161 gcaagacagg aatctcatca attgtccctg catccctgct ctttagccgc ccttgagatg
     5221 ccgtgcgctc tccgccgaaa atccccacag acggcacggc agctctacgg caagaaacgg
     5281 gagggaataa tgaaacagat ttcccgcaga ttgcgcagaa ttgaccgctg atcttgttga
     5341 tttataggga gatagagtaa ctacccgagc aagacgataa aacgttgatt cgtcaaaacc
     5401 ctaaacccct aaaaccctaa tactctataa cctcagcgcc gtcgtgatcc aatctcccgc
     5461 cagggcccaa accaccgcgg tgatggcgat aaaattgccg aaggcaaatt ccatatcgcg
     5521 attgcggata aagatcagaa accagatgat gccagccagg gccgcgaata tgaacaggaa
     5581 gggcatgttc ac
//
LOCUS       DAOSFN010000148         5514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_13074_length_5514_cov_7.874519, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000148 DAOSFN010000000
VERSION     DAOSFN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13074_length_5514_cov_7.874519"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLO35_08465"
     CDS             complement(<1..925)
                     /locus_tag="PLO35_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HOH79742.1"
                     /translation="MRLSDITPYICDFHVHVGERIGGHVLRDGFKDLEPMVQTGELRA
                     IGAFVTEEAGQSLTAKLRSMQKAAREQFSGHVYWHLTPVQASPDEVISLLSEGCDLKL
                     YTTYKSAGLYSSYEDISRWMSDLSGLKTRILIHCEDDATVEEYSDKHPFRHPFQHSLR
                     RPEAAEIIAVERVLDLAARHKHPVHIVHVSSPRSALLIQQARETGVPVTCETAPHYLL
                     LNDEAYYQENAHRLICTPPFRSESSRGMLMELLQDGLFDLVASDHCAFTLEDKDRHQD
                     EPELVPCGIPGIQTLFSSLHQALVQTGKLKLE"
     gene            complement(912..2066)
                     /gene="thrC"
                     /locus_tag="PLO35_08470"
     CDS             complement(912..2066)
                     /gene="thrC"
                     /locus_tag="PLO35_08470"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOH79743.1"
                     /translation="MNHFRHWRCTTCGAEYARDEVRYLCPICSRDYKPGIPLPGVLEA
                     LFDYESIAAEWRKEPDIMLFSALDTNWYPPLPVGKTPLFEVERLGRELGMKRLWIKND
                     ALNPSGSFKDRASQLMVAEARRLGIDEIVCASTGNAASSLASLCASCGIKAVIFTPAA
                     APVAKLTQIKVHGAELHRVDGTYDDAFAQALDYSATHECLNRNTAYHPLTIEGKKSSG
                     LEIFLQLGRVPDWIVIPVGDGVILAGIHKAFVDLHRAGLIDRLPRLLAVQAASSDAIT
                     SYWETGVYHDADQPATVADSISVRTPCNAHWAVKAIKDTYGKAIRVSDLDILNAQKEL
                     AQKTGVFAEPSCSATLAGLKAALGKAWIKASEEALLLITGHGLKDIDALK"
     gene            complement(2063..3256)
                     /locus_tag="PLO35_08475"
     CDS             complement(2063..3256)
                     /locus_tag="PLO35_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOH79744.1"
                     /translation="MFDQIMQKARDYESESVRYLMDMIRIPAFSTKEKEVVEYIKKEM
                     LEAGFDEAFIDPLGNVLGRVGNGPRVIAFDAHIDTVYPGDLKLWDFDPFDAHVKDGKV
                     WGRGSVDQEGGMASMLTAARIIKDLGLNHKFTIWFTGTVMEEDCDGLCWQYIIKEDKI
                     KPEMVVITEPTNMNIYRGHRGRMEMQVHVKGLSCHGSAPERGVNAIYKISRIALEIEK
                     LHERLRDDEFLGKGSVCVTEVYFTGPSQCAVPDSARIHLDRRLTWGETLESSVAEVKE
                     ACRLAGEPDAEIEVLTYEEAAFTGLVYPTQKYFPTWVTPVDSPWLKSAEEAYQKTLGR
                     APKVDKWTFSTNGVAISGMHGIPCIGLGPGNEIYAHAPNEATPIEHLTEAASFYAALV
                     WQLNQ"
     gene            complement(3279..4607)
                     /gene="ssnA"
                     /locus_tag="PLO35_08480"
     CDS             complement(3279..4607)
                     /gene="ssnA"
                     /locus_tag="PLO35_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOH79745.1"
                     /translation="MNSYIITGGTILTFDPAVPVIEDHAILVADGKIIRIAPEAEFSL
                     IQCERIDASGKIVMPGLINAHHHFYSTLVTGLGKAAPSKDFNEVLTNLWWRLDKKLSD
                     DDTYISALISALQAVKKGCTTVIDHHASPWAIPGSLSQIAKATSEAGIRASLCYEVSD
                     RDGEAKALQGIEENAAWISKCDAVKDPMLKGLFGMHAAFTLSDATLSRISSLVKDLGC
                     GTHIHAAEAESDELYNIQHHGKRVVERLHDFGLINANSILAHGVHLNARELMLVASSG
                     AAIVTNPQSNLNNAVGIADLVKMTELGVTVGLGTDAMTVNMLEELRVGLWAQHLRQNN
                     PSAAFMEVASTLLVNNPRIAQKYWGPGHGTITTGGPADLIFVDYDPHTPLDASTWIGH
                     VIYGISQASVNTTICAGKVLLWNGQLMLDLDEAELKARSRSLAKALWDRF"
     gene            4761..>5514
                     /locus_tag="PLO35_08485"
     CDS             4761..>5514
                     /locus_tag="PLO35_08485"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOH79746.1"
                     /translation="MVLQPGTRLRDYEIISLIGTGGMGEVYKAKELTLDREVAIKVLT
                     SRGLDDPLFLQRFVNEAKIHARLSHSNIVDIYTFFEEAKVHYIAMQYAPGITLTELIR
                     TTGLIPEPRALAIFRQICEALAYAHARGVVHRDVKPSNVMVDPQDGDRVKVMDFGIAR
                     LLGAQHITRTGTQMGTVCYMSPEQVLGEKEIDHRSDIYSAGVLLYEMLTGILPYNLDT
                     ESLFKIQNRILQEPLPDPREAYPHISDGTVALL"
BASE COUNT         1126 a         1500 c         1655 g         1233 t
ORIGIN      
        1 tttccagctt gagcttaccc gtctgaacca gggcttgatg cagcgaggaa aagagcgtct
       61 ggatgcctgg aatgccgcag ggcaccagct ctggctcgtc ctgatggcgg tccttgtcct
      121 ccagagtgaa ggcgcagtgg tcgctggcga cgaggtcgaa aagcccgtcc tgcaagagct
      181 ccatcagcat accgcgcgag ctttcgctcc tgaagggagg cgtacagatg aggcgatggg
      241 cgttttcctg gtaataggct tcgtcattga gcaatagata gtggggggcg gtttcgcagg
      301 tgaccggaac gcctgtttct ctggcctgct ggatcaaaag ggctgatctt ggcgatgaga
      361 cgtgcacgat atggacggga tgcttatgac gcgcggcgag gtccaggacc cgctccacgg
      421 cgatgatctc ggccgcttcg ggacggcgca gactatgctg gaagggatgg cggaaaggat
      481 gtttatcgga atactcttcc accgtggcat cgtcctcaca gtggatcagg atgcgggtct
      541 tgagcccgct caagtcactc atccatcggg agatatcctc ataggagctg tagagaccgg
      601 cactcttgta ggtggtgtag agcttgaggt cgcagccttc agacagcagg gatatcacct
      661 catcagggct ggcctgaacg ggagtgagat gccagtatac gtggccgcta aactgctctc
      721 tggctgcctt ttgcatagat cgcagtttgg ctgtgagaga ctggcctgct tcctcagtta
      781 cgaacgctcc gatggccctg agttctccgg tttggaccat gggctcgagg tccttgaagc
      841 cgtccctcag aacgtggcca ccgatgcgct ctcccacatg cacatggaag tcgcagatgt
      901 atggggtgat atcacttaag cgcatcgata tccttcaggc cgtgaccggt gataagcagc
      961 agcgcttcct cgctggcttt gatccaagct ttgccaagag cggctttaag ccctgccagg
     1021 gtagcggaac aggaaggttc ggcaaagacg ccggtctttt gggccagttc tttttgggcg
     1081 ttgaggatgt ccaagtcgct cacgcggatg gctttgccgt acgtgtcttt tattgctttc
     1141 actgcccaat gggcattgca gggagtgcgc acagatattg agtctgccac ggtagcaggc
     1201 tggtcagcat catgataaac gcctgtttcc caataggatg tgatggcgtc agagctggca
     1261 gcctggacgg ccaggagcct gggcagccga tctatcaatc cagcccggtg cagatccaca
     1321 aaagccttgt ggatacctgc cagaatgact ccgtcaccca cggggatgac gatccaatcc
     1381 ggcaccctgc caagctggag aaagatttcc agtccgctgc tcttcttgcc ttcgatcgtg
     1441 agcggatgat aagctgtgtt gcggttcaga cactcatgtg tggcagagta gtctaaagcc
     1501 tgggcaaagg cgtcgtcgta agtgccatcc actctatgca gttccgcgcc gtggactttg
     1561 atctgggtta gtttggccac tggggccgcg gcgggtgtga agatcaccgc tttgatcccg
     1621 caggaggcgc aaaggctggc cagagaggag gcagcgttgc ctgtggaggc gcagacgatc
     1681 tcgtcgatgc ccagccgacg ggcttccgcc accatgagct gggaagcgcg atccttgaaa
     1741 gatccgctgg ggttgagggc gtcgttcttg atccagagcc gcttcattcc cagttcccgg
     1801 cccagacgtt ccacttcaaa caggggggtc ttgcctaccg gcagaggtgg ataccaattg
     1861 gtatccaggg cactgaagag catgatgtca ggttccttac gccactctgc cgctatgctt
     1921 tcataatcaa acaaggcttc cagcactccg ggcaaaggga tgccgggctt atagtcccgt
     1981 gagcaaatag gacagaggta gcggacctcg tcgcgagcgt attcagcccc gcaagtggtg
     2041 cagcgccagt gtctgaagtg gttcattggt taagctgcca gaccagcgcg gcatagaaag
     2101 aggccgcttc ggtgaggtgc tctatcggag tcgcttcgtt gggggcgtgg gcatagattt
     2161 cgttgccagg ccccagaccg atgcagggga taccgtgcat gccggatata gccacgccgt
     2221 tggtgctgaa ggtccattta tccaccttgg gggcgcgtcc gagcgtcttc tgatatgcct
     2281 cttcggcgct cttgagccag ggtgaatcga ctggcgtgac ccaggtggga aagtattttt
     2341 gggtgggata gacgagccct gtgaaggcgg cttcctcgta ggtgagtacc tcgatttcgg
     2401 catccggttc gccggccagg cggcaggctt cttttacctc tgccacagag ctttccagcg
     2461 tttcacccca ggtgagacga cggtccagat ggatgcgagc ggaatccgga acggcacact
     2521 gggaggggcc tgtgaagtaa acctcggtga cgcagacact tcccttgccg aggaactcgt
     2581 catcacggag gcgctcatgc agcttttcga tctcgagagc gatgcggctg atcttgtaga
     2641 tggcgttcac tccgcgttcg ggagcggagc cgtggcagga aaggcctttc acgtgcacct
     2701 gcatttccat gcggccacga tggccccggt agatgttcat attggtgggc tcggtgatca
     2761 ccaccatttc cggcttgatc ttgtcttcct tgataatgta ttgccagcaa aggccgtcgc
     2821 agtcttcttc catcacggtg ccggtgaacc agatggtgaa cttgtgattg aggcccaggt
     2881 ctttgatgat ccgtgcggcc gttagcatgg aggccatgcc accttcctga tcgactgagc
     2941 cgcgtcccca gactttgccg tctttgacgt gggcatcaaa ggggtcgaaa tcccagagct
     3001 tgaggtctcc gggatagacg gtgtcgatat gggcatcgaa agctatgact ctggggccgt
     3061 taccgacgcg gcccaggacg tttccgagcg gatcgatgaa ggcttcgtca aagccggcct
     3121 cgagcatttc ctttttgatg tattccacca cctctttttc cttggtggaa aaggcgggaa
     3181 tgcggatcat gtccatcagg tagcggacgc tttcgctctc gtaatcacgg gctttttgca
     3241 tgatttggtc aaacatatct attctcctgt aatatatctt agaatctatc ccacagcgct
     3301 ttggccagcg accgggagcg ggctttgagc tccgcttcgt cgaggtccag catcaattgg
     3361 ccgttccaca gcagcacttt gcccgcgcag atggtggtat tgacacttgc ctggctgatc
     3421 ccatagatca cgtggccgat ccaggtgctg gcatccagag gggtgtgggg atcgtagtcc
     3481 acaaagatca ggtcggcagg gcctccggtg gtgatggtcc catgcccggg accccagtat
     3541 ttttgagcga tgcgggggtt gttgaccagc agggtggagg ccacttccat aaaggccgcg
     3601 gaggggttgt tctgtctgag gtgctgggcc cagaggccca cgcgcagctc ttccagcata
     3661 ttcaccgtca tggcgtccgt gcccaggccc accgtgacgc ccaattccgt cattttcacc
     3721 agatcggcga tcccgacggc gttgttcaga ttggactggg gattggtgac gatcgccgct
     3781 ccggatgagg ctaccagcat caattcccgg gcgttgagat ggacgccgtg ggccaggatg
     3841 ctgttggcat tgatcaaacc aaagtcatgc agcctttcca cgactcgttt gccgtgatgc
     3901 tggatgttgt aaagctcgtc agattcggct tccgcggcat ggatgtgagt gccgcagccc
     3961 aggtccttta ccagagaact gatcctggaa agggtggcgt ctgagagagt aaaggcggca
     4021 tgcatgccaa agagaccctt cagcatgggg tctttgaccg cgtcgcattt actgatccag
     4081 gcggcgtttt cttcgatacc ctggagggct ttagcctcgc cgtcccggtc tgagacctca
     4141 taacagagcg aagcccggat gcctgcctcg gaggtggctt tagcgatctg agacagcgat
     4201 cctgggatgg cccagggact ggcgtggtgg tcgatcacgg tggtgcagcc tttcttgacg
     4261 gcctgcaggg cagagatcag cgccgagatg taagtgtcat cgtccgagag ctttttatcc
     4321 aggcgccacc agagatttgt gagcacttcg ttgaaatcct tggagggtgc ggccttgccc
     4381 agacccgtca ccagagtgga atagaagtgg tgatgggcat tgatcaatcc tggcatcacg
     4441 atcttgccgg aagcatcaat acgctcgcac tggatgagag aaaactccgc ttcgggggcg
     4501 atcctgatga tcttgccatc cgccaccaga atggcgtgat cttcaatgac cggaacggcc
     4561 ggatcgaaag tgaggatggt cccgccggtg ataatgtatg agttcataat actttccttt
     4621 gcgttttaag taacttgcaa ttgaaataca gggggagaat ttgtcaacta caaatccgcc
     4681 ccagaggcaa acgcaatttc agcttgacca aatccaagcc tccgtttaca gggattttat
     4741 catgattcgg gagttggcca atggtattgc aaccgggaac ccgtctgcgg gattacgaga
     4801 ttatcagcct gatcggaaca ggcggcatgg gagaggtcta taaggccaaa gagctcaccc
     4861 tggaccgcga ggtggccatc aaagtgctca cctccagggg tttggacgat cccctctttc
     4921 tgcagcgttt tgtgaacgag gccaaaatcc acgcccggct aagccacagc aacatcgtgg
     4981 atatctacac ctttttcgag gaagcaaagg tccactacat agcgatgcaa tacgccccgg
     5041 ggatcacgct caccgagctg atcaggacca ccggattgat cccggaaccc agggccctgg
     5101 ccatctttcg ccagatctgc gaagccctgg cctatgccca tgcgcgaggt gtggtgcacc
     5161 gcgacgtcaa gccctccaac gtcatggtcg atccccagga tggcgaccgg gtgaaggtga
     5221 tggattttgg catagcccgc ctgctgggtg cccaacacat cacccgcacc ggcactcaga
     5281 tgggcaccgt gtgttatatg agcccggaac aggtgctggg cgaaaaggag atcgaccacc
     5341 gctccgacat ctattcggcc ggagtcctgc tctatgaaat gctcaccggg attctgccct
     5401 acaacctcga tacggaaagc ctcttcaaga tccaaaaccg catcctgcaa gagcccctgc
     5461 ccgatcccag agaagcctac ccccacatca gcgatggcac cgtggccctg ctga
//
LOCUS       DAOSFN010000149         5455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_13250_length_5455_cov_8.386852, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000149 DAOSFN010000000
VERSION     DAOSFN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13250_length_5455_cov_8.386852"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1898)
                     /locus_tag="PLO35_08490"
     CDS             complement(300..1898)
                     /locus_tag="PLO35_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79747.1"
                     /translation="MKRFLILWIVLLPVLLLGQQGFADEPIVPGDVPGFWEELIESDS
                     LTYGAGGVVGAVTVDGVTYSQIRLRPEIEFGKLGVGLDIDLLIDSDGKIRQEDWNDWK
                     DYLGKLFYIRYGRRKDLLYFKTGAISDYTLGHGMIFNNYSNMLRYPDDKNIGSYVGMN
                     TRLFGLGFELYTHDLFRNQILAGRVHAKPMQYLKIPYFENFKLGFNIGWDRDQLSRFT
                     DDDGDGVPDVYDKFPSDPQSSADTDDDGISDDIDWDINGDGVIDGPANPAVEATFPGI
                     FGYYPDYEFDNDFVTDSLQVYTDKQPLFIYSVDYELPVIDHEYFKLSTYGELAKIQDH
                     GSGLIFPGFGAKFLIFEAKLELRNFGRGFLPSYFDRIYEGKRSELQIVIDPDTGQRHY
                     SLITKEQILDQVKPTMGWFGYMQAELWEMLYFRAAYQDMYGKDMKAGKSIWAKLGATP
                     KMIPKLKQATLYYSQTNVDYIDFRRLRTSQAHVVGELIYAIADNANLIGRYTEHYTDI
                     NNDGRIRGKNEIQEILTFGVEFTF"
     gene            complement(2019..3170)
                     /locus_tag="PLO35_08495"
     CDS             complement(2019..3170)
                     /locus_tag="PLO35_08495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOH79748.1"
                     /translation="MQYTRESLAQMEECYSLRSILIRFSLVLFLFAQTLLVSQPVSDG
                     PENQSPASADSLPGHLTIMAVGDVMLGSNYPSTAYLPPDDGNGLLAPVADMIKAADLA
                     FANLEGVLLTGKGHPKKCGSPSKCYAFKSPDHYIHHFTDAGFDFVSLANNHSNDFGEP
                     GRKNTVRLLDEAGIHHAGLLERPSVIFENKGLKIGFCAFAPNRATVKLNDHKGMKKAV
                     SKLKAQSDLVIVSFHGGAEGKKHQHVTRKNEYFLGENRGNPHKFARDAIDAGADLVLG
                     HGPHVPRALDLYKDRFIAYSLGNFATYSRFNLSGANGLAPMLEIRIDRQGVFQSARII
                     SAIQRGEGGPVPDPDNAAARKISELTEKDFPRGPLEILADGSVTRKSPN"
     gene            complement(3353..4162)
                     /locus_tag="PLO35_08500"
     CDS             complement(3353..4162)
                     /locus_tag="PLO35_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015591752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOH79749.1"
                     /translation="MEENKDDLIKANLDKIKHRVIVMSGKGGVGKSFIAVNLAYGLAM
                     QGKKVGLLDVDIHGPSIAKLTNIEGMPIPVNQETGRPTPIKALSNLSVLSIASLIKTE
                     DSAIIWRGPMKMSLIKQFFSDFDWEELDYLIIDCPPGTGDEPLSIVQTLGKLDGAVIV
                     TTPQDIAILDVRKSVDFAHKLDLPILGVVENMKFFRCPKCGEVTPIFQGEQLEKMIFE
                     HSLDLLAALEIDPAIARSADEGKPYIYFYNKLPSAQALMTAVEKLMEKCES"
     gene            complement(4364..4726)
                     /locus_tag="PLO35_08505"
     CDS             complement(4364..4726)
                     /locus_tag="PLO35_08505"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOH79750.1"
                     /translation="MKIAIPVSNNELSPHFGHCHAFAVFTVENKQVVASEIVTPPTHE
                     HGSNPLFLQSLACDVVISGGMGKHAHEMLQSMNIQVFQGDASQSPAELVNLFLNGKLQ
                     AGSGSCGHHEGQDHAHTH"
     gene            complement(4776..5222)
                     /locus_tag="PLO35_08510"
     CDS             complement(4776..5222)
                     /locus_tag="PLO35_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOH79751.1"
                     /translation="MPRAKSLRIMQELPIVKGFRPLWMRANYRSAVVMALEEYEVIRL
                     VDYEKLTHEQASESMGVSRPTLTRIYELARSKLATAIIEGRSFLIEGGEVRISGSHYF
                     CEDCQHKFVHNEDESKPQHCPACGSGKIICLSECFMTGCRRCSRCR"
BASE COUNT         1297 a         1401 c         1423 g         1334 t
ORIGIN      
        1 gcaaccaaga taaccctgac aggagagggc aaggctttta tctgtgtgac agcgccagga
       61 aattgagaat tgacaattga caattgacaa ttgagaattg agacttgaga attgacaaat
      121 ggggcttatg gataattgag agtgtaatgg tggatgccga actgggatgg ctctcaacca
      181 gcacgcagtg ctctcaccta cgcagtatct cacctacgca gtatctcacc taccgcaggt
      241 atctcacagc gcagctctca cagcgcagct ctcacagcct cagctctcag ctctcagctc
      301 taaaacgtaa actccacccc aaaggtcaga atctcctgga tctcattctt gccacggata
      361 cggccatcgt tgttgatatc agtgtagtgc tcagtatagc ggccgatcag gttggcgtta
      421 tcggcgatgg cgtaaatcag ctcacccacc acatgggcct ggctggtcct gagacggcga
      481 aaatcgatgt aatcaacatt ggtctgtgag taatacaagg tggcctgttt caacttgggg
      541 atcattttgg gggtggcccc cagcttggcc cagatcgatt taccggcttt catgtcttta
      601 ccatacatgt cctgataggc agcccggaaa tacagcatct cccaaagttc ggcctgcata
      661 tagccaaacc agcccatggt aggcttcacc tgatccagga tctgttcttt ggtgatcagg
      721 gagtaatgcc gctgaccggt atccggatcg atgacgatct gcagttcgct gcgcttgccc
      781 tcgtagatgc ggtcaaaata ggagggcaga aagcctctgc caaaattgcg cagctccagc
      841 ttggcttcaa agatgaggaa tttagcgcca aaacctggaa agatcaggcc tgacccgtgg
      901 tcctggattt tggcaagctc gccgtaggtg ctcagtttaa agtattcatg gtctatcaca
      961 ggaagctcgt aatctacgct atagataaac aaaggctgct tgtctgtata gacctgaagc
     1021 gaatcagtca caaaatcgtt gtcaaactcg taatccggat agtatccaaa gattcccggg
     1081 aaggtggctt ccacggcagg attggcggga ccatcgatca ctccatcacc gttgatgtcc
     1141 caatcaatat cgtccgaaat cccgtcgtca tccgtatcag cgctggattg gggatccgag
     1201 ggaaacttat catagacgtc cggaacgccg tcgccatcgt catcagtgaa acggctcaat
     1261 tggtcgcggt cccagccgat attgaagccc agcttaaagt tttcaaaata tggtatcttc
     1321 agatattgca tgggcttggc gtgcacacga cctgccagga tctgattgcg gaagagatcg
     1381 tgggtgtaaa gttcaaagcc cagaccaaac agcctggtgt tcatgcccac atagcttccg
     1441 atgttcttgt catccgggta acgcagcata tttgagtagt tgttgaaaat catgccatgg
     1501 cccagcgtgt agtctgagat cgcgccggtt ttaaagtaga gaaggtcttt gcgcctgccg
     1561 taacggatat agaaaagctt gccgagatag tctttccagt cgttccagtc ttcctggcgg
     1621 atttttccat cgctgtcgat caataaatct atatccaggc cgacgccgag cttgccaaac
     1681 tcgatctcag gacgcaggcg gatctgggaa taagtcactc catcgacggt cacggctccc
     1741 accacgcctc ccgccccata ggtgagggaa tcggattcga tcaattcttc ccaaaagcct
     1801 ggcacgtctc ccgggacgat tggttcatcg gcaaagccct gctgtcccaa caacaggacc
     1861 ggcagcagga caatccacag gatcagaaac cttttcatgg tttacctcct cttcgcaggt
     1921 aaggatcctc taattcagac ttgtggtgct gtcaagagaa atcgctcaaa ggggaggtcc
     1981 aaggcgccta caatgagccg ttaagtggaa ttgtgccatc aatttgggct ttttctggtg
     2041 acgctgccat cggccaatat ctccaagggc cccctgggga aatccttctc cgtaagctca
     2101 gatatcttcc tggcagccgc attgtctgga tctggcacag gtccaccttc accgcgctgg
     2161 atcgctgaaa tgatccgggc agattgaaac acgccctgcc ggtctatcct gatctctagc
     2221 atgggggcca gaccattggc cccgctcagg ttgaagcggg aataggtggc aaaattgccc
     2281 agggaatagg ctataaacct gtctttatag agatccagcg ctctgggaac gtgaggtccg
     2341 tggcccaaaa caaggtctgc tccggcatcg atggcatccc tggcaaactt gtggggattt
     2401 ccacgatttt cgcccaaaaa atactcgttc ttgcgggtga cgtgctgatg ctttttgccc
     2461 tcggccccgc cgtggaagga aacgatcacg aggtcggact gggctttgag cttgctcacc
     2521 gcctttttca tccccttgtg gtcgttgagt ttgacggtcg cccggttggg agcgaaggca
     2581 caaaagccga tcttcaggcc tttgttctca aagatcacgc tgggcctttc caggagaccc
     2641 gcgtgatgta tgcctgcctc atccagcaag cggaccgtat tcttgcgtcc cggttcacca
     2701 aagtcattgg agtggttgtt ggcgaggctc acaaagtcaa acccggcgtc tgtgaaatga
     2761 tggatataat gatccggaga tttgaaagca tagcatttgg acggggaccc acactttttg
     2821 ggatgccctt taccggtgag caggacgccc tccagattgg caaaagccag atcggcagct
     2881 ttgatcatat cagccacggg ggccagtaaa ccattgccgt cgtcgggagg caggtaagcc
     2941 gttgaagggt agttggagcc cagcatgacg tccccaacag ccattatagt gagatggccc
     3001 ggaagtgaat ccgcgctggc ggggctctgg ttttcggggc cgtcgctgac aggctgggag
     3061 accagcaggg tctgggcaaa cagaaacaac accaggctga acctgatcag gatagacctc
     3121 agggagtagc attcttccat ctgggcaagc gattctcggg tatactgcat caggctctcc
     3181 aatcgtatta actagatagt ccgacatgcg gatacatgca aatcaggctc caagtgcatg
     3241 attatcggag gctggaaacg agtcaaggat ttgctgaagc aatctttgaa tacgtgaaaa
     3301 aaaagacagc ctgatgcttc aggctgtctt tatctgggtg gagtgtgatc ctttaggatt
     3361 cgcatttctc cattagtttt tccacggccg tcatcaaggc ctgggcggaa gggagtttgt
     3421 tgtagaaata gatatagggc ttgccctcat cggccgatcg ggcaatggcg ggatcgatct
     3481 ccagggctgc cagcaggtcc agcgaatgtt caaagatcat cttctccagt tgctcaccct
     3541 ggaagatggg agtcacttct ccgcattttg ggcagcggaa aaacttcatg ttttccacca
     3601 ctcccaaaat gggcagatcc agcttgtggg caaaatccac agatttccgg acatccagga
     3661 tggcaatatc ctgcggagtg gtaacaatga ccgctccatc cagctttccc agcgtctgaa
     3721 caatgctgag gggctcatca ccggtaccgg gagggcaatc gatgatcaga taatccagct
     3781 cctcccaatc aaaatcactg aagaactgct tgatcaggga cattttcatg ggtcccctcc
     3841 agatgatggc gctatcctca gttttgatca gggaggcgat ggaaagcacg ctgagattgg
     3901 ataaagcttt gatgggagtg ggcctgccgg tttcctggtt gacagggatg ggcattcctt
     3961 cgatattggt gagcttggcg atcgaaggac cgtggatatc cacatccagg aggccaacct
     4021 tcttgccctg catggcaagt ccataagcga gattgacggc tatgaagctt ttgcccacac
     4081 cgccttttcc actcataacg atgacgcggt gtttgatctt gtccagatta gctttgataa
     4141 ggtcgtcttt gttttcttcc attacttgct taccttctcg agttgttcgt ttatccattg
     4201 cagccgcgct tccaactcct gtttgcgtgc caggagctca gcacggttat agcaatagat
     4261 tgtgtctgtg tccggaactg gctcctggag aaggttttgc ccaatgaagc gtcctctggg
     4321 ggccggctga ctgtgacaac ggtgtcgata acggcggcca ggctcagtga gtgtgcgcat
     4381 gatcttgtcc ttcatgatgt ccgcaagatc cgctgccggc ctgcagcttc ccgttcagaa
     4441 agagattcac gagctctgcg gggctctggg acgcgtctcc ctgaaacacc tggatattca
     4501 tgctctgaag catctcgtgt gcatgcttgc ccattcctcc ggaaataacg acgtcgcagg
     4561 cgagtgactg tagaaaaagg ggattagaac catgttcatg agtgggtggt gttactatct
     4621 cgctggcaac cacttgcttg ttttcgacgg tgaaaacagc gaaggcatgg cagtgaccga
     4681 aatgggggct aagctcattg tttgagactg ggatggcgat tttcataagg gcttcctttt
     4741 tggatttatc atcaatatgc gcgattatat aatgatcatc gacagcgcga gcatcgtctg
     4801 cagcccgtca taaaacactc gctcaggcag atgatcttgc ctgaaccgca ggctgggcaa
     4861 tgctgtggct ttgattcgtc ttcgttgtga acaaacttat gctggcagtc ttcgcagaaa
     4921 taatggctgc ctgagatcct cacctctcct ccctcaatca ggaagctgcg tccctcaata
     4981 atagccgtgg caagtttgga gcgggccaat tcatagatcc tggtgagagt aggacgcgac
     5041 acgcccatgc tttcggaggc ctgctcatgg gtgagctttt cgtagtccac caggcgaata
     5101 acctcatact cctccagggc cataaccacg gcagaacggt agttggcacg catccagaga
     5161 ggacggaatc ctttcacgat cggcagctct tgcatgatcc tcagcgattt tgctcgtggc
     5221 atcgtaactc cttgccgtca agaatagtgc ttgcatctat aatgaacata tgttcaaatg
     5281 aaatgaaccc ggttgatctg tcaagtcttc ttttcgatgt gtggcttact ccatagagat
     5341 tttatccaaa ctctcccccc atcacccaca gtggagatac cgtggggccg ggggcgaaaa
     5401 atataaaccc tcactgaaaa cttcaaatcc aatcaccaaa acttcaaatg tccaa
//
LOCUS       DAOSFN010000150         5284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_13861_length_5284_cov_9.869956, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000150 DAOSFN010000000
VERSION     DAOSFN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13861_length_5284_cov_9.869956"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            647..1027
                     /locus_tag="PLO35_08515"
     CDS             647..1027
                     /locus_tag="PLO35_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOH79752.1"
                     /translation="MTELRQIYHCPLCGNVVEVLYTGAGELVCCGEPMNLLKENSVDA
                     AREKHVPVLSDLGGKVKVAVGSVAHPMEAKHYIALIEVITEKCVYRHELQPGEVPEAI
                     FPVSMDEVLYAREYCNLHGLWKNA"
     gene            1060..1650
                     /locus_tag="PLO35_08520"
     CDS             1060..1650
                     /locus_tag="PLO35_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOH79753.1"
                     /translation="MSFKETRTAENLMKSFAGESQARMRYVYSAKTAKKEGFEQICNI
                     FMETAENEKEHAKVFFKHLLKNGLEGQVINIMASYPVGWSPETTLKNLEYAANGEKEE
                     WTELYPMFADIAEEEGFKDIATAWRMIAKVEKEHEKRFRKLYENIKNMRVFEKDGSVF
                     WKCLNCGYIHEGATAPKTCPACQHPQSYFEIHQETY"
     gene            1662..1805
                     /locus_tag="PLO35_08525"
     CDS             1662..1805
                     /locus_tag="PLO35_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOH79754.1"
                     /translation="MQKYQCIACGWIYDPAQNDNIPFEELPEDFVCPECGVGKDMFEP
                     YEE"
     gene            1875..2327
                     /locus_tag="PLO35_08530"
     CDS             1875..2327
                     /locus_tag="PLO35_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOH79755.1"
                     /translation="MTIQEFHEILNFAVDREREAVKFYHDLQSVAKFQDQIAILKDLE
                     AMETGHITVIENIRAKGVKEEDIPKVPNLKISEYITVDADKLDLSYQNILIKAMKREE
                     ASFKLYSEMSVKFGDSEIATLFRKLASDEAKHKLIFERYYDEWISAGN"
     gene            2306..2989
                     /gene="tsaB"
                     /locus_tag="PLO35_08535"
     CDS             2306..2989
                     /gene="tsaB"
                     /locus_tag="PLO35_08535"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOH79756.1"
                     /translation="MDKRGQLILALDASQPSGSIALWSADSLIYSALFDISVTHSETL
                     MPQVDQALRFCGCLPSDLQAVLLANGPGSFTGLRIGLATAKGIAYALKIPLRVFSSLK
                     LAALPRAGCGKPILSVIDARMKEVYAALWDEHLNPIIAPCVCLPEEIAGWDCPECWVL
                     GNGSHLLTQSPKMAFAPDLVTGFVPAAGLRALDMLEPGSQEYDFERIADLEPFYLRDS
                     SAQVKRHPQ"
     gene            3049..3546
                     /locus_tag="PLO35_08540"
     CDS             3049..3546
                     /locus_tag="PLO35_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH79757.1"
                     /translation="MQASQFEAFLQNNQKRIYHYLISLTGNEPDAQDLVQIVFIAFYE
                     HIDRVEEATALAYLYKIAHNKCMTFLKQKSRYISADPKSFDHIPDRPQNSPEPDFSPL
                     RDAVQALPPRLASVIQLQYYEKLSYKEISTQLGISVKAVESLLVRAKKILRKKLVKEI
                     QGMGV"
     gene            3603..4043
                     /locus_tag="PLO35_08545"
     CDS             3603..4043
                     /locus_tag="PLO35_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOH79758.1"
                     /translation="MRCAKYRHYIDLLPDGNLDKAAAQRLDAHLKDCVSCRVYHDQGL
                     RLQQLIQSAPQAPFPDWLHQRILHDCRAHEVQRSGFRKRARWQLVPAAMAVMLSLFLG
                     SLIGKTAFDTQTQTAASSSQTSDELSFGEITLVDSDYYSGGANE"
     gene            4036..4509
                     /locus_tag="PLO35_08550"
     CDS             4036..4509
                     /locus_tag="PLO35_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOH79759.1"
                     /translation="MNKRWLLILLLISAAFNLAVLGSFVYLRGFHPRHHHSPQREWRD
                     KDRWPGRFHRGPAPEFAFGDSIRALRQGFEDTKRELMLELAKDPLDMPQIEAIITRSL
                     NAQVALERDMADRLIRFRNTLTPEEAREHFSRRAEDMQRRDNDNHKYTKSRRSRR"
     gene            4506..5177
                     /locus_tag="PLO35_08555"
     CDS             4506..5177
                     /locus_tag="PLO35_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79760.1"
                     /translation="MKKFSIITLTLMLLTGLLFAQGAPMKHKGMQVPGSPADNPKLKA
                     RLAPEDAPMPMIEMLKLSKEQQAKFQDLRLEHQKRMNLLEAEVENLKLDIHKMIKGEQ
                     FADAKKLNDSLYAKKKDMANARIDHMQAMLKELSAEQKEIAKEHFMMMGMMGRKGHMM
                     SRGQGMGHMGQMGMRNHDGNCGEGMGRMHGQKNKMQDCEDCEDCEDKAPDTKNKLPND
                     TPKQK"
BASE COUNT         1421 a         1421 c         1344 g         1098 t
ORIGIN      
        1 cttccgctgt gtcagggttt ccccttgatt tttgtttttt gttctttttg ccttatgcaa
       61 atttcagggc gaagggacaa agctatgaat ctgcgatccc gtgggggtgc agggggattt
      121 ttccccctgc ctaaagtatt tagacagcta tatcaaggtg aggacgttgt agctctgaag
      181 ccgttataac gtggatattt ttctattgct atactttata gtatggcaga acctggggaa
      241 caaagcgcca gatctatgtc attgctatca atagttttcg ggcgctttgg acttcaaaca
      301 gcagatcgga ggaggttttc tcgcgagtgt gctggttgaa gtccaagcgg cccgtaaact
      361 cgctgtcata tatacaatta ttgaggccgc ttgaactcca acggggtcat cagcattcaa
      421 tcaaatcaat tctctgtttc tctgtccctc cgtccctctg ttttaaaaaa agggaaaacc
      481 ccatcaccac agatattcca tatacataag ccccaaaact ccaacctatc caatctaccc
      541 ccccaaaaaa aacgagcctc tgttttaact ccacctgaga tttctgttga caaaagaagc
      601 ccacctttca cccattgaaa aaagcaaaaa atacctggag gtaaagatga cagaactgcg
      661 tcaaatctac cattgtcccc tgtgcgggaa cgtagtcgaa gtgttatata ccggagcggg
      721 cgagctggtc tgctgcgggg aaccgatgaa tcttttaaag gaaaactctg tggacgccgc
      781 gcgtgaaaag catgtgcccg tgctcagcga tttgggtggc aaggtcaagg tggccgtggg
      841 ttccgtggcc catcccatgg aagccaagca ctatatcgcc ctcatcgagg tgatcacaga
      901 gaagtgcgtg tatcgccatg agctccagcc cggagaggtt ccggaagcga ttttccccgt
      961 gagcatggac gaggtgcttt acgcccgcga atactgcaac ctgcatggcc tttggaaaaa
     1021 cgcctgaact gattattata cttgatagga gaacccaata tgtcttttaa agaaacccgc
     1081 accgccgaaa atctgatgaa atcctttgcc ggtgagagcc aggcccggat gcgttacgtg
     1141 tattccgcca agacggccaa aaaggaaggt ttcgagcaga tctgcaacat ctttatggaa
     1201 accgcggaaa acgagaagga acacgccaag gtctttttca aacacctgct caagaacggt
     1261 ctggaaggcc aggtgatcaa catcatggct tcctatccgg tgggctggag tccggaaacc
     1321 accctcaaaa acctggaata tgccgccaat ggcgaaaaag aggaatggac ggagctctat
     1381 cccatgtttg ccgacatcgc tgaagaggaa ggctttaagg atatcgccac cgcctggaga
     1441 atgatcgcca aggtggagaa agaacacgaa aagcgcttcc gcaagctgta tgaaaacatc
     1501 aaaaacatgc gcgtctttga aaaggacggc agcgtattct ggaaatgcct caactgcggc
     1561 tacatccacg aaggcgccac cgctcccaag acctgcccgg cctgtcagca tccccagtcc
     1621 tattttgaaa tccatcagga aacctactaa gggaggataa aatgcagaaa tatcaatgca
     1681 tagcctgcgg ttggatctac gatcccgccc aaaatgacaa tatcccgttc gaagaactgc
     1741 ccgaagactt tgtctgcccc gaatgcggtg tgggcaaaga catgttcgag ccctacgaag
     1801 aataggccat caaccggttc cggaagctcc ttacagggct tccggagctc atttatcacc
     1861 gtgtgtgagg aataatgacc atccaggaat ttcatgaaat actcaatttt gccgtggatc
     1921 gcgaacgcga agcggtgaag ttttatcacg acctgcaatc agtggccaaa tttcaggacc
     1981 agatcgccat cctcaaagat ctggaggcga tggaaaccgg ccatatcacc gtgatcgaaa
     2041 acatccgtgc caaaggcgtg aaagaagagg acatccccaa ggtgcccaac ctcaagatca
     2101 gcgaatatat caccgtggac gcggacaaac tggacctcag ctatcaaaac atcctgatca
     2161 aggccatgaa gcgggaagag gcttccttca agctctacag cgagatgagc gtcaagtttg
     2221 gagacagcga gatcgccacc ctgtttcgca aactggcctc cgatgaagcc aaacacaagc
     2281 tcatcttcga gcgctattat gatgaatgga taagcgcggg caactgatcc tggcgctgga
     2341 cgcgtcccag ccctctgggt ccatagctct ttggagcgct gatagtttga tctacagcgc
     2401 tctttttgat atcagcgtca cccacagcga gactttgatg ccgcaggtgg accaggccct
     2461 caggttttgc ggctgtctgc cctccgatct gcaagccgtg ctgctggcca acggtcccgg
     2521 gtcattcact ggcctgcgga ttggtctggc caccgccaag gggatcgcct acgcgctcaa
     2581 aatccccttg agggtgttca gttcgctcaa gctggcggct ttgccgaggg caggatgtgg
     2641 aaaacccatc ctcagtgtga tcgatgccag gatgaaggaa gtgtatgcgg cgctgtggga
     2701 tgaacacctg aatccgatca ttgcgccctg cgtgtgtttg cctgaagaga ttgccggttg
     2761 ggactgccct gagtgctggg tgctgggcaa cggcagccat ctgctgaccc aaagcccaaa
     2821 aatggctttt gccccggact tggtaacggg ctttgttccc gcggctggac tgcgagcgct
     2881 ggacatgctg gagcccggca gccaggaata tgactttgag cggattgccg atctggagcc
     2941 cttttatctg agagactcct cggcccaggt gaaacgtcac ccgcagtgat gccagagatc
     3001 aatgcagcgg aagaggaacc gtccccgttt gcgatgaaac aagtttagat gcaagccagc
     3061 cagtttgaag ctttcctgca aaacaaccaa aagcggatct accactatct gatctcgctg
     3121 acgggcaacg agcccgatgc ccaggacctc gtccagatcg tgtttatcgc cttctacgaa
     3181 cacatcgacc gggtggaaga agccaccgcg cttgcctatc tatataagat cgcccacaac
     3241 aaatgcatga cttttctcaa gcaaaaaagc cgctatatca gtgccgatcc caaatctttc
     3301 gatcacattc cagaccgtcc ccaaaactcc ccggaaccgg atttcagtcc tctccgggac
     3361 gcagtccagg cattgccacc ccgcctggcc tccgtgattc aactgcaata ctatgaaaag
     3421 ctaagctata aagagatttc cacccaattg gggatcagcg tcaaagcggt ggaatcgctg
     3481 ctggtgaggg ccaaaaaaat cttaaggaaa aaactcgtga aggaaatcca ggggatggga
     3541 gtataaacaa tgacatacaa tcagtcagca ccaccaaggt gccactggaa aaggaatcta
     3601 ccatgagatg tgccaaatat cgccactaca tagacctgct gccggacggg aacctggaca
     3661 aggctgccgc ccagaggctg gatgcgcatc tcaaggattg tgtcagttgc cgggtttatc
     3721 atgatcaagg ccttagactg cagcaactta tccaatcagc accgcaagct cctttcccag
     3781 actggctgca tcagagaatc ctgcacgact gccgtgccca cgaagtccaa aggagcggtt
     3841 tccgcaaacg ggccaggtgg cagcttgttc ctgccgccat ggctgtgatg ctgagcctct
     3901 ttttgggttc tctgatcggc aaaaccgcct tcgacaccca aacccagacc gccgcgagca
     3961 gttctcagac aagcgatgag ctcagctttg gagaaatcac gctggtggat agcgactatt
     4021 atagcggagg ggccaatgaa taagcgctgg ctgctcatcc tgctgctgat ctcagcggct
     4081 ttcaacctgg ccgtgctggg cagctttgtc tatctgcgcg gtttccatcc gcgtcatcat
     4141 cactccccac aacgcgagtg gcgggataaa gaccgctggc cgggaagatt ccatcgcggt
     4201 ccggctcccg aatttgcctt tggagacagc atccgcgctc tcagacaagg ctttgaggat
     4261 accaagcggg aactgatgct ggaactggcc aaagacccac tcgatatgcc ccaaatcgaa
     4321 gcgatcatca cgcgttccct gaacgctcag gtagcgctgg aacgggatat ggcagaccgt
     4381 ctgatccgtt ttcgcaatac cctgacgccg gaagaggcac gtgaacattt tagccgccgc
     4441 gcggaagata tgcaacggcg cgacaacgat aaccataagt acacaaaatc aaggagatca
     4501 agaagatgaa gaaattctca ataatcaccc tcaccctcat gttgctcaca ggtttgctgt
     4561 ttgcccaggg cgctcccatg aaacacaaag gcatgcaagt gccaggctca cctgccgata
     4621 accccaagct caaagccagg ctcgctcctg aagacgctcc catgcccatg atagagatgc
     4681 tgaagctcag caaggaacaa caggccaagt ttcaggacct gcgcctcgag catcaaaaac
     4741 gcatgaacct gttggaagcc gaagtggaaa acctcaaact ggacattcac aaaatgatca
     4801 aaggcgagca gtttgccgat gccaagaagc tcaacgatag cctctatgcc aagaagaaag
     4861 atatggccaa tgcccgcatc gatcacatgc aggcgatgct caaagaactg agcgccgaac
     4921 agaaagagat tgccaaagaa cactttatga tgatgggaat gatgggccgc aagggccaca
     4981 tgatgtcgcg cggccaaggc atgggccaca tgggccagat gggtatgcgc aaccacgatg
     5041 gcaattgcgg tgaaggaatg ggcaggatgc atggccagaa aaacaagatg caagactgcg
     5101 aagactgcga agattgcgaa gacaaagccc ccgataccaa aaacaaactg ccgaacgaca
     5161 ctcccaaaca aaagtaataa tctccccagt tagagacaca taaacgccgc ggtatcaaaa
     5221 ccgcggcgtg ctatttccgg gcaaaaacca ggcacggtct aagagacctc tcctgtcgct
     5281 ggcc
//
LOCUS       DAOSFN010000151         5284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_13862_length_5284_cov_5.431631, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000151 DAOSFN010000000
VERSION     DAOSFN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13862_length_5284_cov_5.431631"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLO35_08560"
     CDS             complement(<1..326)
                     /locus_tag="PLO35_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOH79761.1"
                     /translation="MGLPHQLSEYKGKVIMIVNTASKCGFTKQYADLQKLWETYGKDG
                     LVILGFPANNFMNQEPGSDTEIQNFCQLNYGVSFPMFAKISVKGKDIHPLFAYLTDKK
                     AGHDHGG"
     gene            complement(655..1995)
                     /locus_tag="PLO35_08565"
     CDS             complement(655..1995)
                     /locus_tag="PLO35_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79762.1"
                     /translation="MKHAVLLMLMLAGLAGLMADGNALFFDGSNDVVELANPGPTNII
                     TAECWFQPLTLGTGDLGRTILSSNPAFNKSMWLTSVGTELRLWMFGSSNNSLSFTTSG
                     ANIQVGNWYHVAITGQRGAANRTILYLNGVEMINQPSYDLSNFGISFYFGNLRSGASS
                     TYAFHGYIDEVRVWTTIRSQSDILNNMNTIVPLDSPGLVGYWPLDSSNGATVYDMVEP
                     AANGTVQNGTTPNPNPAFAPSDVTLPVELTSFMATGTSDFFIRLTWITHSEAGVAGYY
                     VYRNDVLDFGSAYLISDLIEAQNQTSTTQYSYIDDEVSPGTWYYWLQHLDYNGDDVVY
                     GPVTYTLSEPGEGSDTPGLDAITDFLPVYPNPFNPSVFFPYLLETRMPVKLQVVNLRG
                     QVVKSYDLGDQETGLHTVAWDGTDTNGRKLSTGIYLVRLQAGDKLFQRKAVMVK"
     gene            complement(2201..4147)
                     /locus_tag="PLO35_08570"
     CDS             complement(2201..4147)
                     /locus_tag="PLO35_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79763.1"
                     /translation="MDRIKCPFCKEEIFADAQKCRFCGEWLHKASVEDNATLAQVRQA
                     FASHYRVVAELGRGGMAIVYQADQLTLERSVALKVVPREFSHDADFVARFYREAKNSA
                     KLQHPNIVTIHEVGEQGGFPFMALEHLAGGTLSELIGRHGRFSEAGIAEIILPVLEGL
                     SYAHAQGIIHRDLKSSNIMFDLKGRPVIMDFGISRGLDSGKLTKTGTIIGSPDYMSPE
                     QAQGLDSDVRSDIYSIGVVMYEMACGKVPFAADSLVGLIYKVVNQAPELDLLRGTHSD
                     AFIGILEQCLAKQPQERFSTAQALISALRHGSSGGQQPLYNTLNPVMNAPRPAPGNTQ
                     TEDGSAVPIYGEAASPAAKSKLPMLIFIIGFILLAATVLLLSLKPKAEELPPEATAVV
                     DSTPAPAFVEEAATPAPRLIGIPMVKVAGGEYLMGDVFDIGNEDEKPIHQVRLSDFFL
                     GQFEVTQGEWKQVMGSNPSTFQGDDRPVENVTWLQAVQFCNALSATEGLDSCYLISAD
                     RVSLDPGANGYRLPTEAEWEYAARGGNNHSDFYFAGSDDLDSVSWYGSKAWTLSVGKL
                     QSNELGIHDLSGNIWEWCWDWYASSYPQTGSTNPRGPNSGSLKVLRGGGWGATPNQCR
                     NTSRHSSSPGHKHGSIGFRVCRNG"
     gene            complement(4219..5151)
                     /locus_tag="PLO35_08575"
     CDS             complement(4219..5151)
                     /locus_tag="PLO35_08575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79764.1"
                     /translation="MLADENQTTRVQTTPPRRPRIRIVEVLMIALGIIMILMAAYLIF
                     QKVKDKATPEPPAGQGDPNFVWVEGGQFIMGIEAGQETDSQPAHPVRVPSFYMSAREV
                     TQAEWMEVMGGNPSQFKHPQNPVEQVDWYDAIRYCNLRSLKEGLTPCYSISGNTSPSS
                     WSSGSIAWNPSADGYRLPTEAEWEFAAKGGMQSSSYLYSGSNEAALVCWYNAESSVPQ
                     PVGTKQPNELGIHDMSGNVWEWCWDWFAPYNPEASTHPMGPSSGAMKILRGGSYIYDA
                     GLCRVFFRSYRGPSYKFGNVGFRVCSNRQPQARN"
BASE COUNT         1125 a         1459 c         1501 g         1199 t
ORIGIN      
        1 ccgccatgat cgtgaccggc ctttttatcc gtcagatagg caaagagggg atggatgtcc
       61 ttgcctttga ccgagatctt ggcaaacatc gggaagctga ccccgtagtt gagctggcag
      121 aagttttgga tctcggtatc gctgccgggt tcctggttca taaagttgtt ggccgggaag
      181 cccaaaatca ccaggccatc ctttccatag gtttcccaga gcttctgcag atcggcatac
      241 tgcttggtga agccgcattt gctggccgtg ttgacgatca tgatcacctt gcccttgtac
      301 tcagaaagct ggtgtggcag gcccatgatg tccgttactt catagtcgta gatgtgcttg
      361 tgttctggca tggtcgtttc tccccaaagc aaggctgcca ggcccagcag aaggatcggg
      421 ccgagaatga gggtgagctt tttcatagga tttacctgct ttgtgtgttg tgatttgttt
      481 tgaggatatt gtagtcaata ttagacacca ggcaaggagt gaggcaatct ggcgatgccc
      541 tggcccaaaa gaacggtccc ggcatgccag attaacctgc cgggaccgtt gcgacctcct
      601 cagttgaggg gaggccaatg tttcacgctg agatactggg ctggcggaaa tcctctactt
      661 gaccatcacg gctttgcgct ggaaaagctt gtcgccggct tgcaggcgca ccagatatat
      721 gccggtggaa agcttgcggc cgttggtatc ggtcccatcc caggcgacgg tgtgaagccc
      781 tgtttcctgg tctcccaggt catagctctt taccacctgt ccgcgcaggt tcactacctg
      841 cagtttcacc ggcatgcggg tctcgagcag gtaggggaaa aagacactcg ggttgaaggg
      901 attgggataa acgggcagaa aatcggtaat ggcgtccaga ccaggggtat cggaaccttc
      961 accgggttcc gaaagtgtat atgtgaccgg gccatagacc acgtcgtcgc cattgtagtc
     1021 cagatgctgg agccaataat accaggtgcc gggtgacact tcatcgtcta tatagctgta
     1081 ctgggtggtg ctggtctggt tctgcgcctc gattagatcg ctgatcaggt acgcgctgcc
     1141 aaaatccagc acatcgttgc gatagacata atatccagcc actcctgcct cggaatgagt
     1201 gatccaggtg aggcggataa agaaatcgga ggtgccggta gccataaagg aagtgagttc
     1261 caccggcagg gtgacatcgc tgggcgcgaa agcgggattg ggattggggg tcgtgccgtt
     1321 ttggacggtt ccgttagcgg cgggctcaac catgtcatag acagtcgctc cgttggagct
     1381 gtccagcggc cagtatccta ccagaccggg tgaatccagc ggcacgatgg tgttcatgtt
     1441 gtttaggata tcactttgcg atctgatggt ggtccacacc cgcacttcat cgatatagcc
     1501 atggaaagcg taggtggagc tggccccgct gcggaggttg ccaaaataaa agctgatgcc
     1561 aaagttgctc aaatcataac tgggctgatt gatcatctca accccattga gatagaggat
     1621 agtgcggttg gcggctcccc gttgacctgt gattgccaca tgataccagt tgcccacctg
     1681 gatgttggcc ccgctggtag tgaagctcag actgttgttt gagctgccaa acatccagag
     1741 acgcagctcg gtacccactg acgtgagcca catggattta ttgaaggctg gattggagga
     1801 gagaatcgtc ctccccaaat cccccgttcc cagggtcaga ggctgaaacc agcactcggc
     1861 ggtgataatg ttggtgggcc cgggattggc cagctcgacc acatcattgg agccgtcaaa
     1921 aaaaagggcg ttgccgtcgg ccatcagtcc agccaggccg gccagcatca acataagtaa
     1981 aacagcgtgt ttcataagtt cctccagaac aaaaaaacca aatctgcctc gcaaccttgg
     2041 tgggaagcga gggcgtcaaa ccgacgcgta gtcactggat agcattcgca aaatttgtca
     2101 agtaatatct tgacaatcca gccacgacaa cggtgataac tgctcacaag agtggccagc
     2161 tctcgtccac cgcacggagt cccgccagga aacatggtgc tcagccattc ctgcacaccc
     2221 taaacccgat gctgccatgc ttgtgaccgg ggctgctgct gtgacggctg gtgttgcggc
     2281 actggttggg tgtggcaccc cagccccctc ccctgaggac tttgaggctg ccggaattgg
     2341 ggccccgggg attggtggac ccggtttgcg ggtaagagga tgcataccag tcccagcacc
     2401 attcccagat gtttccgctc aagtcatgga ttcccagttc gttggattgc agtttgccca
     2461 cgctgagggt ccaggccttg ctgccatacc aggagacgct gtccagatcg tcgctgccgg
     2521 caaaatagaa gtcactgtgg ttattgccac ccctggcagc gtattcccat tccgcttctg
     2581 tgggcagccg gtatccattt gcccctgggt ccaggctcac gcggtcagcg ctgatcaggt
     2641 aacagctgtc caggccttcc gtggcactga gggcgttgca aaactggact gcctgcagcc
     2701 aggtgacgtt ttccacgggg cggtcatccc cctgaaaagt ggagggattg gagcccatga
     2761 cctgcttcca ctcgccctgc gtgacctcaa actggcccaa gaaaaagtcg cttaagcgca
     2821 cctggtggat aggcttttcg tcctcgttgc cgatgtcaaa gacgtctccc atcaagtatt
     2881 cacctcctgc caccttcacc atggggatgc caatcagacg cggggctggc gttgccgctt
     2941 cctccacaaa tgccggagca ggggtggaat cgaccacggc cgtggcttct ggaggcaatt
     3001 cttcagcttt gggcttgagg ctcagcagca gcaccgtggc ggccaggagg ataaacccga
     3061 tgatgaaaat gagcataggg agcttgcttt tagcagcggg agaagcggct tcaccgtaga
     3121 tgggaacagc actgccatcc tctgtttgag tattccccgg cgccggtctg ggcgcattca
     3181 taacagggtt aagagtatta tacagaggct gttggccgcc agaactgcca tgccgcagag
     3241 cggagatcaa tgcctgggcc gtgctgaacc tctcctgagg ctgcttggcg aggcactgct
     3301 ccagaatccc gataaaggca tccgaatggg ttccacggag cagatccagt tccggggcct
     3361 ggttgaccac cttgtagatc agccccacca gcgaatccgc cgcgaagggt actttcccgc
     3421 aggccatctc atacatgacc accccgatgg aatagatatc gctccggacg tctgaatcca
     3481 ggccctgggc ctgttcaggg ctcatgtaat ccggagaccc gatgatggtg cccgtcttgg
     3541 tgagcttgcc actgtccagg cctctggaga tgccaaaatc catgatcacg ggacggccct
     3601 tgaggtcgaa catgatgttg ctgctcttga ggtcgcgatg gatgattcct tgggcgtgag
     3661 cgtaggaaag gccttccagg acgggcagga taatctcggc aatcccggct tccgaaaatc
     3721 tgccgtgacg gccgatcaac tcgcttaaag tgcccccggc aagatgctcc agcgccataa
     3781 agggaaaacc accctgctcg cccacttcat ggatggtgac gatattggga tgctgcagtt
     3841 tggccgaatt cttagcttcg cggtagaaac gggctacaaa gtccgcgtcg tgactgaact
     3901 cccggggcac cactttaagc gccacgctgc gttccagggt caattgatcg gcctgataca
     3961 cgatcgccat gccacccctg cccagctctg ccaccactct gtagtggctg gcaaaagcct
     4021 ggcggacctg ggccaaagta gcgttatcct ccacggaggc tttatgcagc cattcaccgc
     4081 aaaagcggca tttctgggca tccgcgaaga tctcttcttt acaaaagggg cattttatcc
     4141 tgtccatgaa tcctgattcc ttcctttggc tgttggtctc ctaaagtgcc atctcggttg
     4201 ttatccaagc tcccgcattc agttccgtgc ctggggctgg cgatttgagc agactctaaa
     4261 gcccacattg ccaaacttat agcttggtcc gcggtaggaa cgaaagaata cccggcacaa
     4321 tccggcatca tagatatacg agccacccct caggattttc atcgcgccgc tggaggggcc
     4381 catgggatgg gtgctggctt ccgggttgta gggcgcgaac cagtcccagc accactccca
     4441 gacgtttccg ctcatgtcat ggatgcccag ttcgttgggc tgtttggtgc ctacgggctg
     4501 aggaacggag ctttcggcat tgtaccagca taccagcgca gcttcgttgc tgccgctgta
     4561 gaggtatgag ctgctttgca tgcctccttt ggccgcgaat tcccattccg cctcggtggg
     4621 aaggcgataa ccatccgcgc tggggttcca tgcgatactg ccgctgctcc aactggaggg
     4681 actggtgttg ccgctgatgg aatagcaggg ggtgaggccc tctttgagac tgcgaaggtt
     4741 gcaatagcgg atcgcgtcat accagtcaac ctgttccacc gggttttggg gatgcttgaa
     4801 ctgggaagga tttccgccca tcacctccat ccactcagcc tgggtaactt cccgggcaga
     4861 catataaaaa gacggcaccc tcacgggatg ggccggctgc gaatcggttt cctgcccggc
     4921 ttcgatgccc atgataaact gacccccctc cacccacaca aagtttgggt ctccttgtcc
     4981 ggctgggggc tctggagtgg ctttgtcttt gactttttgg aagatcagat aagccgccat
     5041 caggatcatg ataatcccca aagcaatcat caggacttcc acgatccgga ttctgggtct
     5101 gcggggcggg gtggtctgaa cccgcgttgt ctggttttca tctgctagca taaaacctgc
     5161 ctaacttatt ataatggagc tacatcctgc aaataacact ggctcttccc cccggaaccc
     5221 tgaagggaag aagcggcatg ctcgcaataa agcttgccgc gccgcgcttg tcaagcaaat
     5281 tgtc
//
LOCUS       DAOSFN010000152         5260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_13948_length_5260_cov_6.278386, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000152 DAOSFN010000000
VERSION     DAOSFN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13948_length_5260_cov_6.278386"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(374..1033)
                     /gene="phoU"
                     /locus_tag="PLO35_08580"
     CDS             complement(374..1033)
                     /gene="phoU"
                     /locus_tag="PLO35_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOH79765.1"
                     /translation="MLSQKLTELKKLLMAEASLVEKMVAISMNAIMGGYECNLMEVNH
                     FEKRVNQLELEIESFCTQLIALYQPEAKDLRAILMSYKINNDLERLGDQAVNITESAN
                     AICGHPIVQELPELDSMRKSALNMLTKSISAFTDKDVELARKVCQDDSIVDDLNRAII
                     SKLIAMMKDNPQQIDVYLHLMRISRNLERVADLSTNVAENTVFLAQGKVIKHHVEEQE
                     G"
     gene            complement(1065..1823)
                     /gene="pstB"
                     /locus_tag="PLO35_08585"
     CDS             complement(1065..1823)
                     /gene="pstB"
                     /locus_tag="PLO35_08585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOH79766.1"
                     /translation="MVKTHICVQKLNLWFGDNHVLHDIDLCLYEHKVTAMIGPSGCGK
                     SSLLRCFNRMNDLIPDTRVKGKILLDDVDIYDPKARPTDIRARVGMVFQKPNPFPKSI
                     FANVAYGLSIQGKFSRSEISQRVEKSLRDAWLWDEVKDRLSSPAMSLSGGQQQRLCIA
                     RALANQPRILLLDEPTSALDPQSTAKIEELCLALKDQVTILIVTHNIAQAGRISDYTA
                     FMYLGELVEFDQTSQMFTVPKDSRTEAYLTGVFG"
     gene            complement(1735..2670)
                     /gene="pstA"
                     /locus_tag="PLO35_08590"
     CDS             complement(1735..2670)
                     /gene="pstA"
                     /locus_tag="PLO35_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOH79767.1"
                     /translation="MTRRELKNKAGQGLLALMLLITAALLLIFLFSIFKRGLGVISWE
                     FVTQPPRNGMTAGGIFPAIVGTFWLTLLSILIALPLGILCAVYLHSYGRPRWFMRAVK
                     VAIDTLAGIPSIIYGLFGMALFVNMLGMDVSMLAGSLTLAVLALPLIINASEEAMRGV
                     PKDFIEASIALGATQRQTILRVILPTALPNILTGAIISIGRVAGETAPIMFTAATFYS
                     RRLPRSVTDEVMALPYHIFALMTEGSKGAQQVPSPTEALWCCFCWCWEFHRRQSSSAI
                     GSGGPDNGKDSYLRTEAQPLVWRQPRITRYRSLSL"
     gene            complement(2667..3539)
                     /gene="pstC"
                     /locus_tag="PLO35_08595"
     CDS             complement(2667..3539)
                     /gene="pstC"
                     /locus_tag="PLO35_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOH79768.1"
                     /translation="MNDFKEKSFKLITYIAALASIMLLLGIIFSVFAEGIPLFKTIKI
                     AEFLFGRDWYPTHGEAPQFGIWNLLIGSLLATFGALVIAVPLGLGSAIFISEIAGSRL
                     REIAKPVIELLAAIPSVVYGLFGMAVLAPFVRTLFNVSTGLNLLSTSIILGLMVVPII
                     ASMSEDALSTVPKQLREASLALGATPWETISKVVVPAARSGIVGSVLLGFGRAIGETM
                     VVLMVAGGSAQIPRSIFDSLRPLTSTIAAEMGETVVGDLHYRSLFAIAILLFIITFVT
                     NMFTEIVILKRGKA"
     gene            complement(3630..4442)
                     /locus_tag="PLO35_08600"
     CDS             complement(3630..4442)
                     /locus_tag="PLO35_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOH79769.1"
                     /translation="MRKYIIILLTLVLSFAALSLAAKGKQITCSGSTTVLPIAQAAAE
                     AYMDTHPDLNISVRGGGSGVGVAALQNGTVQIANSSRPIKSKEISACKAKGVDPRAYV
                     IANDAIAIVVHKGNAVQNLSIKQIKDIYTGKITNWKQVGGPSLPIVVVSRDVASGTFE
                     VFNEKALGGAKVAASAQLLASNNAVVTAVSTTPGAIGYAGLGYITDGVKVVKVENVTP
                     SETTAKNGTYKLARKLYMYTNGKAKGDVGNFIGWLQSPAGQEIVKQQGFISL"
     gene            complement(4457..>5260)
                     /locus_tag="PLO35_08605"
     CDS             complement(4457..>5260)
                     /locus_tag="PLO35_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79770.1"
                     /translation="PIPDAKISAGLQKVYFGSIYDWDYTLIGKAPSDEYKVANSADYG
                     LTVNGYLPSGFGEYAVGIYNGEGYKNFGANLKDNTSFEYLANLRLTPIRGITLGGSFM
                     TNSAEREKALATDADVAGYQKHTLMDGLARFVYGPVDVMAEYLMKDVEYPNVAKGSKD
                     YTANGLSIIPILNLRHFIDTDIQIVGRYDRWDETDNASAKHLLTAVTGGVNYNFMHND
                     SYVPAFQLQFNVTQKTYDPDESAAAYANNLKDSLTVMAQLKWRFSNNLK"
BASE COUNT         1141 a         1383 c         1498 g         1238 t
ORIGIN      
        1 ccgcaaactc gttgccatgt atagctttat cgtggccgct tgaactccaa cggggatcgg
       61 cccgcaaact cgttgccatg tatagcttta tcgtggccgc ttgaactcca acgggacacg
      121 gaggagcgat tctccctgcg gtgtgctggt tgaagtccaa gcggcccgca aactcgctgc
      181 catatatact cttatcccgg ccgcttgaac tccaacggat caactccaac ggggctatgt
      241 cagcctccgg cttcgccagt ttttgcacaa gcgctgaagc cagtgatacc gattatccca
      301 tggccttctc atcgtcccat accctctcat cctctcaagt tctcattgta tggaagactc
      361 accgaggctc agttcagccc tcctgttcct ccacatggtg cttgatcacc ttgccctggg
      421 ccagaaagac ggtgttttcg gccacgttgg tggataggtc tgccactctt tccaggtttc
      481 gcgagatgcg catcaggtgg aggtaaacgt ctatctgctg ggggttatct ttcatcatgg
      541 caatcagttt ggagatgatg gcgcggttca ggtcgtccac gatgctgtcg tcctgacaga
      601 ccttgcgggc cagttccacg tccttgtccg tgaaggcgct tatgcttttg gtgagcatat
      661 tgagggcgct tttgcgcatg ctgtccagct cgggaagctc ttgaacgatg gggtgaccgc
      721 agatggcgtt ggcgctttcg gtgatattga ccgcctggtc gcccaggcgt tccagatcgt
      781 tgttgatctt atagctcatg aggatggcac gcaggtcttt ggcttccggc tggtagagag
      841 caataagctg ggtgcaaaag ctctcgatct ccagttccag ctggtttacg cgtttttcaa
      901 agtggtttac ttccatgagg ttgcactcat atccgcccat aatggcgttc atcgagatgg
      961 ccaccatctt ttccaccagg gaggcctcgg ccatcagcag tttcttgagt tcggtcagtt
     1021 tctggcttaa catgatatct cctgaggata aaataagctg ctgttcatcc gaaaacacct
     1081 gtcaaatagg cttcggtgcg actgtctttg ggaacagtga acatctggga ggtttggtca
     1141 aattccacca gttcgcccag atacataaat gccgtgtaat ctgatattct gccggcttgg
     1201 gcgatgttgt gggtcacgat caggatcgtc acctgatctt tgagggccag gcagagctct
     1261 tcgattttgg cggtggattg agggtccaga gcggaagtgg gctcatccaa cagcaggatg
     1321 cggggctgat tggccaaagc tctcgcgatg cagagcctct gttgctggcc tcccgaaagg
     1381 gacattgcgg ggctggacag ccggtctttc acctcgtccc aaagccaggc gtctctgagg
     1441 ctcttttcca cccgctggct gatctcgctc cgggagaatt tcccctggat ggacagtccg
     1501 taggccacgt tggcaaagat gctcttggga aagggattag gcttttgaaa caccatcccc
     1561 acccgggccc tgatgtccgt tggacgggct ttgggatcat atatatccac atcgtcaagg
     1621 aggattttcc ccttgacgcg ggtatccgga atgaggtcgt tcatgcggtt gaaacagcgt
     1681 agcaggctgg attttccgca tccggaggga ccgatcatgg cggtcacctt atgttcataa
     1741 agacagagat cgatatcgtg taatacgtgg ttgtcgccaa accagaggtt gagcttctgt
     1801 acgcaaatat gagtctttac cattgtctgg tcctcctgat ccgatagcgg atgatgattg
     1861 ccgccgatga aactcccagc accagcagaa gcagcaccac agcgcttccg taggcgatgg
     1921 gacttgctgg gcccccttgg aaccctcagt cataagcgcg aaaatatgat aaggcagggc
     1981 catcacttcg tctgtgaccg agcggggcag acgtcggctg taaaaagtgg cggcggtgaa
     2041 cattatcggg gccgtttctc cagccacgcg accgatgctg atgatagcgc cggtgagaat
     2101 attgggcaac gcggtgggca gtattaccct caggatcgtc tgtctttggg tggcccccag
     2161 agcgatggag gcctcgatga agtcttttgg caccccgcgc atggcttctt ccgaggcgtt
     2221 gatgatcagc ggcagcgcca gcaccgccag agtgagcgac cctgccagca tggacacatc
     2281 catgcctagc atgtttacaa acagcgccat gccaaagagg ccgtagatga tggaagggat
     2341 gccggccagg gtgtcgatag ccactttgac ggcacgcata aaccagcgcg gcctgccata
     2401 actgtgcagg tagaccgcgc aaagaatgcc cagcggtaaa gcgatcagga tggaaagcag
     2461 ggtgagccag aaagtgccaa cgatggcggg aaagatccct ccagccgtca tcccgttccg
     2521 gggtggctgg gtgacaaact cccaggagat cactcccaga ccgcgtttga aaatactgaa
     2581 gaggaagatc aacagcagcg ctgcggtgat caacagcatt aaggccagca gaccttgccc
     2641 ggccttgttt ttgagttccc ggcgcgtcat gctttgcccc tcttcaggat cacgatctcg
     2701 gtgaacatat tggtgacgaa cgtgatgata aacagcagga tagctatggc gaaaagggag
     2761 cggtagtgca ggtctcccac cacggtttct cccatttcgg cagcgatcgt ggaagtaagc
     2821 ggccgcaggg aatcaaagat gctcctggga atttgggccg atccgcctgc caccatcaac
     2881 accaccatcg tttctccgat ggcgcgtcca aagcccaaca gcaccgatcc cacgatccca
     2941 ctccgagcgg cgggcaccac cactttgctg atcgtctccc aaggagtggc ccccaaagcc
     3001 agagaagctt ctctaagctg tttgggcaca gtggaaaggg cgtcttcgct catggacgcg
     3061 atgatcggca ccaccatcaa acccaatatg atggaagtgc tgagcaggtt cagaccggtg
     3121 gacacattaa acagggttct gacaaaaggt gccagcaccg ccatgccaaa gagcccgtaa
     3181 accacactgg gaatggcagc caaaagctcg atcaccggtt tggcgatctc acgcagacgg
     3241 ctgccggcaa tttcggagat aaagatcgcg gagcccaagc caagtgggac cgctatgacc
     3301 agcgctccaa aagtggccag cagagagccg atcagtaggt tccagatgcc aaactggggc
     3361 gcttccccat gagtgggata ccagtccctg ccaaacagga attcagctat cttaatggtc
     3421 ttgaagagtg ggattccctc ggcaaagacg ctgaaaatga tccccagcag caacatgata
     3481 ctggccagcg ccgctatata ggtgatcagc ttgaagctct tttctttaaa atcgttcatc
     3541 tattatccct tacacagaga cgaacgggcc actgctgacc acgttcgtct gaggttttgg
     3601 tgttttcttt gggggcttga gagcatcgct tagagggaga taaatccctg ctgttttaca
     3661 atttcctgtc cggcggggga ttgcagccag ccgatgaagt ttcccacatc gcctttggcc
     3721 ttgccgttgg tgtacatata gagcttgcgg gccagcttgt aagtgccgtt tttggcagtg
     3781 gtttcggaag gagtcacgtt ttccactttg accaccttta ctccgtctgt gatgtaaccc
     3841 agtccggcat atccgatcgc gccgggcgtg gtgctaaccg ctgtcacaac cgcattattg
     3901 gaagccagaa gctgagccga ggccgccact ttggcaccgc ccagagcttt ttcgttgaag
     3961 acttcgaagg tgccggaagc cacgtcgcgg gagaccacca cgatcggcaa actgggtccg
     4021 cccacctgct tccagttggt gatcttaccg gtgtagatgt ccttgatctg cttgatgctg
     4081 aggttctgaa cggcatttcc tttgtgcacc acgatggcga tagcgtcgtt ggcaatcacg
     4141 taagcccggg gatcaacgcc tttggccttg caggcggaga tctctttgga tttgatgggg
     4201 cgtgaggaat tggcaatctg gacggtgccg ttttgcagag cggcgacgcc tactccactg
     4261 ccgccaccgc ggacggagat attgagatcg ggatgggtgt ccatataggc ttcggccgcg
     4321 gcctgagcga tgggaagcac ggtggtggag ccggagcagg tgatctgctt gcctttggca
     4381 gccaggctga gagcggcaaa actgaggacc agggtgagaa ggatgatgat gtatttacgc
     4441 atggtaataa ctccttttat ttcaggttgt ttgagaagcg ccacttgagc tgcgccatca
     4501 cggtgaggct gtctttcaaa ttgttggcat aggcagcggc ggattcatcg ggatcgtagg
     4561 ttttctgggt gacgttgaac tgcaactgga acgcgggaac gtagctgtcg ttgtgcatga
     4621 agttgtagtt tacaccaccg gtaacggctg tgaggagatg tttggcggaa gcgttgtcgg
     4681 tctcgtccca gcggtcatag cgtcccacga tctggatatc ggtatcgata aagtgacgca
     4741 ggttcaggat ggggatgatg ctgaggccgt tggcagtgta atctttgctg cctttggcca
     4801 cgttgggata ttccacgtct ttcatcaggt attcggccat cacgtccaca ggaccgtaaa
     4861 cgaaacgggc caggccgtcc atgagcgtat gtttctgata tccagccaca tccgcgtccg
     4921 tagccagagc tttttcacgt tcggcgctgt tggtcatgaa agatccaccc agagtgatac
     4981 cgcggatggg agtcaggcgc aggttggcca ggtattcaaa gctggtgttg tccttgaggt
     5041 tagcgccaaa attcttgtag ccttctccat tatatatacc cacggcgtac tcaccaaaac
     5101 ccgaaggcag gtagccattt accgtgagac cgtaatcggc actgttggca actttgtatt
     5161 catctgacgg ggctttaccg atcagagtat aatcccagtc gtagatgctg ccgaaataca
     5221 ctttctgcag accggcagag atcttggcat cgggaattgg
//
LOCUS       DAOSFN010000153         5242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_14005_length_5242_cov_10.747253, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000153 DAOSFN010000000
VERSION     DAOSFN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14005_length_5242_cov_10.747253"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLO35_08610"
     CDS             complement(<1..298)
                     /locus_tag="PLO35_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79771.1"
                     /translation="MNRYYDWKDVANAQFGKAVTLSLVLLTFVMLVTPNIEARKQKFT
                     ASQTELVDIPMEERERLEPPPTEVQIEVPIVISDELSSDNDPALTAKYEQALAEI"
     gene            complement(311..742)
                     /locus_tag="PLO35_08615"
     CDS             complement(311..742)
                     /locus_tag="PLO35_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOH79772.1"
                     /translation="MAKIKQTKKREAFIPTGSMSDIAFLLLLFFMVSTVFVQEKKFWV
                     ERDRWPVAQSIERIPRNNTTTIYVMKNETILLDDVPTKIESEEDVMIANALRSKKSEN
                     PEIVVCFRTDRETKYGAMSDVMRQLQNAETLVVAFEAQKRQ"
     gene            complement(753..1178)
                     /locus_tag="PLO35_08620"
     CDS             complement(753..1178)
                     /locus_tag="PLO35_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOH79773.1"
                     /translation="MKVGRKRKGKAEIPTSSMSDIAFLLIIFFMATTKFDVKEGIKLV
                     LPAAAPESAQQAETIQLGDDKMTRLQITADGSIAINKDGARKIDNAELDSIIQQKLRV
                     NDQMIFKVITDREAQYNEMIRVVDRLKAQKAEKISLSTN"
     gene            complement(1191..1943)
                     /locus_tag="PLO35_08625"
     CDS             complement(1191..1943)
                     /locus_tag="PLO35_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOH79774.1"
                     /translation="MNKKTTWIVLLIMAMTMLFAVLGAQEAAAEVTEEAASGSKNIAE
                     FVFGSGMVKWFKDGGWAMWPILLVTIYGMAYVIWKFIALIHGKINLNNFLGRIVPLIK
                     DKKYKEAVELAAKTRGPVAAMLHAGLVKVDRGVDAVEKSMENAAMIEMSYLEKGFLEL
                     STAITLAPMLGFLGTVAGMISAFDAIAAARAVDATIVASGIKIALITTEAGLIVAIPV
                     QFFNNIFTTMVDGLVIDMQRASEKVIEALIES"
     gene            complement(1972..4950)
                     /locus_tag="PLO35_08630"
     CDS             complement(1972..4950)
                     /locus_tag="PLO35_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH79775.1"
                     /translation="MRKYALILLILIGSMALLNAATTGRLAVRVRDNGGRPIEFVNVA
                     VLQGGRSITGGQTNNKGTAIIINIPPGFYTVKFTLIGFDTYTFNDVRIQVDQTTSLSP
                     VMNKTGIRLNAVVVTAEQDRVEKDRVGSAKQIEMTNMSDVAVSDVAGVVSLQAGVTNI
                     GGELHIRGGRANEVNYTVDGMSVSDPVDGGSALGVDLDAISDMKVMTGGFPAEYGNAQ
                     SGVINIVTKDGDPFYSGKIEYNTDHLIGSGRNSDVIKFAFGGPVWPIGNDDLKEKFTF
                     YFNGGGEWQDGRLKEYWVGNPNRDYVYNGVQLLEYEYEPYDPYEDRSSILGVDLGNRN
                     YNGYNINFKTKYVFNPTQRITFAMRGDRNYDHSFAYNWRYALHHYNISEVKQSQYIGT
                     YDHVFSSSMNLKLKASFYQKTSVAGPRGIDRNNYLYRNPIEADTYVDNVLMGDYGYSS
                     VDNNADGIYDEGFLPASFWTYRVSGVEDPRAITGFVAPGTISDSFVDDVTSSTNLRAD
                     FEYQVNETHLAKTGLEIIKHNIEKNQLQSFLTIYEDRRQASLNRVYDSINLASWNPGD
                     PIPSQLYDVFVTDDGVKIPIYKPEDYFRAAQEASGKRDGYQAEPWQMAWYLQDKMEWE
                     GMIVNAGLRFDFWYLGSQYKVLQDNGSFRTVDFDSNERFQAMLSPRLGVSHPITERDV
                     LRFAYNYQNQLPQMQFIFTSKTPADANVSDTAITVGNTSLEPQITVTYEVGLSHQLSD
                     DYVIDMTAYYKNLYNYVSTVKEKKPGEEQITWYKFISEDYGSARGIDIQLEKVLSNFT
                     NWTIAYSLAWAQGNNSSTVIQDEMTNLREFPLDWDVRHNLNTSFTFRIGKGEEFFVPF
                     TNFILPMDDFSANLTWSFASGSPYTPQAEIGTNFLDTNSLRKDWTNQANLRISKGITL
                     PKDMSMSVFLDVENLFKTKNVLTVYPRTGSPYETGDDISDSTTGYTYPEVAYVYDRAI
                     RNPGYVNNHRGVTLGLSFKF"
BASE COUNT         1104 a         1305 c         1474 g         1359 t
ORIGIN      
        1 cgatctcagc cagggcctgt tcatacttgg cagtgagggc gggatcattg tctgagctga
       61 gctcgtcgct gatcacgatg ggcacctcga tctgtacttc ggtgggtggg ggctcgaggc
      121 gttcccgctc ttccatgggg atgtccacca gctcagtctg agaggcggtg aacttctgtt
      181 tacgggcttc gatgttgggg gttaccaaca taacgaaggt aagcaacacc aaagacaggg
      241 ttactgcttt gccaaactgg gcgttggcga cgtctttcca atcgtagtat ctattcacca
      301 tatcgcgcct ctattgccgc ttttgggcct caaaggccac taccagagtt tcagcgttct
      361 gaagctgacg catcacgtcg gacatggcgc catacttggt ttcgcggtct gtcctgaagc
      421 agactacgat ctccggattc tcggatttct tgctgcgcag cgcgttggcg atcatgacgt
      481 cttcttcaga ttcgatcttg gtggggacat catccaggag gatagtctcg tttttcatca
      541 cgtagatggt ggtggtgttg ttgcggggga tgcgctcgat gctttgcgcg acgggccaac
      601 ggtcccgctc cacccaaaac ttcttttcct ggacaaacac ggtggaaacc atgaagaaca
      661 gcagcagcag gaaggcgata tcggacatgg agccggttgg aataaaggct tcacgttttt
      721 tggtttgttt gatctttgcc acctgagctc ctttagtttg tggatagaga gatcttctcg
      781 gctttttggg ccttgagtct gtccactacc cggatcattt cgttgtactg agcttcacgg
      841 tcggtgatca ctttaaagat catctgatcg tttaccctca gcttctgttg aatgatgcta
      901 tccaactcgg cattgtcgat cttgcgggca ccatccttgt tgatggcgat actgccgtcc
      961 gctgtgatct gaagacgagt catcttgtca tctcccaact ggatggtctc tgcctgctga
     1021 gcactttccg gagctgccgc gggcaggaca agcttgatac cttccttcac gtcgaacttg
     1081 gtggtcgcca tgaagaagat aatgagcaga aaggcgatgt cggacatcga cgacgtcggt
     1141 atctcggctt tgcctttcct cttgcggccg actttcatct ctacccccta tcaggactcg
     1201 atcagggctt caatgacttt ctcggaggcg cgttgcatgt caataaccag gccgtccacc
     1261 atggtcgtga agatattgtt gaagaattgg acagggatag caacgatcag accggcttct
     1321 gtggtgatca aagcgatctt gataccggag gcaacaatgg tggcatcaac cgcacgggca
     1381 gccgcgatag catcaaaagc tgagatcata ccggccacag tacccaggaa tcccaacatg
     1441 ggagccaaag tgatggcggt tgagagttcc aaaaagcctt tctcaagata gctcatctcg
     1501 atcatggcgg cgttttccat gctcttttca accgcgtcca cgcctctgtc cactttcaca
     1561 agacctgcat gcagcatggc ggcgacagga ccacgagtct tggcggccag ctccaccgct
     1621 tctttatact tcttgtcttt tatcagaggg acgatgcgtc ccaaaaagtt gttgagattg
     1681 atcttgccgt ggatgagggc gatgaacttc cagatgacgt aggccatgcc atagatggtc
     1741 accagcagga tcggccacat ggcccatccg ccgtctttaa accatttcac cattccgctg
     1801 ccaaacacaa actcggcgat gttcttgctt ccacttgcag cttcttctgt tacttccgct
     1861 gcggcttcct gggctcccag gaccgcgaac agcatggtca tcgccatgat cagcagtact
     1921 atccatgtgg ttttcttgtt cattatgtga ttcctccttc ggaattgaaa tttagaattt
     1981 gaatgacagg ccaagcgtga caccacggtg gttgttcaca tagccggggt ttctgatcgc
     2041 ccggtcgtaa acgtaggcga cctcgggata agtgtaaccg gtcgtggagt ccgagatatc
     2101 atctcctgtt tcataagggg aaccggtacg ggggtagacg gtgaggacgt ttttggtctt
     2161 gaagagattt tccacatcca ggaatacgct catgctcata tccttgggca gggtgatgcc
     2221 tttggatatc ctgaggttgg cctgattggt ccaatccttg cggagggaat ttgtatccag
     2281 gaagttggtg ccgatttctg cctgaggtgt atagggagaa ccggaggcaa agctccaggt
     2341 gaggttggcg ctgaaatcat ccataggcag gataaagttt gtgaagggca caaagaattc
     2401 ctcacccttg ccgatacgga aggtgaagct ggtattgagg ttgtggcgta cgtcccaatc
     2461 cagagggaac tcacgcaggt tggtcatttc atcctggatg acggtgctgg agttgttgcc
     2521 ctgggcccag gcgagggagt aggcaatggt ccagtttgtg aaattggaga gcaccttctc
     2581 cagctggata tcgataccac gggcggagcc gtagtcttcg gagatgaatt tgtaccaggt
     2641 gatctgttct tcaccgggtt tcttctcttt gacggtgctc acgtagttat agaggttctt
     2701 gtaataggca gtcatgtcga tcacatagtc atccgaaagc tggtgggaaa ggcccacttc
     2761 gtaggtgacg gtgatctgag gctccaggct ggtatttccc actgtgatag ctgtgtctga
     2821 gacattggcg tcggcgggag tcttgctggt gaagatgaac tgcatctgcg ggagctggtt
     2881 ttgatagttg taggcaaaac gcagcacgtc gcgctctgtg atgggatggg acacacccag
     2941 gcggggtgaa agcatagcct ggaagcgctc gttggagtca aaatccacgg tccggaagct
     3001 gccgttatcc tgtaagactt tatattggct gcccagatac cagaaatcaa agcgcaggcc
     3061 ggcgttcacg atcatacctt cccactccat tttgtcctgg agataccagg ccatctgcca
     3121 gggttcggcc tggtagccgt cccgctttcc agatgcttcc tgagcggcac ggaaatagtc
     3181 ttccggcttg tagatgggga tcttgacgcc gtcgtcggtc acgaacacgt catagagctg
     3241 gctggggatg gggtcgccgg gattccagga ggccagattg atgctgtcgt aaacgcggtt
     3301 aagactggcc tgacgcctgt cttcatagat cgtgaggaag ctttgaagct ggtttttttc
     3361 gatgttgtgc ttgataatct caagcccggt cttggccagg tgggtctcgt tgacctgata
     3421 ctcgaaatcg gcgcggaggt tggtggacga agtgacgtcg tctacaaagc tgtcgctgat
     3481 ggtgccaggg gctacaaagc cagtgatggc gcgcggatct tccactccgg agacgcggta
     3541 ggtccagaag ctggcgggca ggaatccctc atcgtagatg ccgtcggcgt tgttgtccac
     3601 gctggaatat ccataatctc ccatgaggac gttatccaca taggtgtcgg cttcgatcgg
     3661 attgcggtag agatagttgt tgcgatcgat accgcgggga ccggcaacac tggtcttctg
     3721 gtagaagctg gctttgagct tgaggttcat ggaggagctg aagacgtggt cgtaggtgcc
     3781 gatgtactgg ctctgcttta cttcgctgat gttgtagtga tgcagggcgt aacgccagtt
     3841 ataggcaaag ctgtggtcat agttgcggtc tcctcgcatg gcgaaggtga tcctctgggt
     3901 agggttgaag acgtatttgg tcttgaagtt tatattgtag ccattgtaat tgcggtttcc
     3961 cagatcgacg cccaggatgc tgcttctgtc ttcataggga tcatagggct cgtattcata
     4021 ttccaggagc tggacaccgt tgtaaacgta gtcccggttg ggatttccca cccaatattc
     4081 tttgagacgt ccgtcctgcc attcaccgcc accgttgaag tagaaagtga atttttcttt
     4141 gagatcatcg ttgccaatag gccagacggg accgccgaag gcaaacttga tgacgtcgga
     4201 attgcggccg gaaccgatca ggtggtcggt attgtattct atcttgccac tatagaaggg
     4261 atctccgtct ttggtgacga tgttgatcac gcctgattga gcgttgccat attcagccgg
     4321 gaagccaccc gtcatcactt tcatgtcgct gatcgcgtcc aggtccacgc ccagtgcgga
     4381 accaccgtcc acggggtcag aaacgctcat accgtccacg gtgtagttca cctcattggc
     4441 gcgaccgccc cggatgtgaa gctcgcctcc gatgtttgtc acaccggctt gcagggagac
     4501 cactccggcc acatcggaga ccgccacgtc ggacatgttg gtcatctcga tctgtttggc
     4561 ggatcccacc ctgtcttttt ccacccggtc ctgctcagcc gtaaccacga ccgcgttgag
     4621 cctgatcccg gttttattca taacgggaga gagggacgtg gtctggtcca cctgaatgcg
     4681 gacatcgttg aaggtgtagg tgtcaaaacc gatgagggtg aatttgacgg tgtaaaaccc
     4741 gggagggatg ttgatgatga tggccgtgcc tttgttgttg gtctggccac cggtgatgga
     4801 acggccgcct tggaggacgg caacgtttac aaattctata ggcctgccgc cgttgtcgcg
     4861 aacgcggaca gcgagccggc cggttgtggc ggcattgagc aaggccatgc tgccgatcag
     4921 aatgagcagg atcaatgcat atttacgcat cgataaacct cccttatctc taagcattat
     4981 ttctgtttgc aatagcttac ctgatcaagt ggtgattcta cgcggatact ctttcagggg
     5041 taggtcacgt taatctggta taagcaaaat agtcaagcag ttttttggat agtttttgcc
     5101 gaaggccgag gcaaagcgtc aacagctcca taatcacgtc ccaaaatcat tctgaatcgc
     5161 tccacaatct gcctgcctcc cttatccgcc ccgcatccta ttacggactc attacggact
     5221 tagtccgtaa tgagtccgta at
//
LOCUS       DAOSFN010000154         5227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_14066_length_5227_cov_8.674594, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000154 DAOSFN010000000
VERSION     DAOSFN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14066_length_5227_cov_8.674594"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2613
                     /locus_tag="PLO35_08635"
     CDS             <1..2613
                     /locus_tag="PLO35_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOH79776.1"
                     /translation="DHGYVEYSTDGGYNWYIFPASSYRGTGIYDTPLQNNPEGPCFDQ
                     ASYPLWSSYTGSGFLNGYLVTDTFDLSPWAGCEDFRVGYRAVWDGESGGMGWYIDDFS
                     ISIQAPGVPHTPLPVSGTTGFGTFETINWTGINSSSYDLTLHTDPGFPGFVTLSANSY
                     PCVDLAPNTTYFWKVRGHNSVGTSDWSPVWSFTTGSYRSTRHNASSLWISEVSFGSIN
                     NSSGWNGYTYYSGIGTNLTGGSSTPIQVSLAGGYGPEAVGVWIDLDRDHVFGSTPAEF
                     TQLAWNGSGFGGNIVIPPAQSPGPTRMRIQGIHTDNTDVLTPTGIFNFGETEDYDLQL
                     YTAPTLSISPSSGDFPPTMQHWQSSPIRFTFSNIGSGSLDITLTGITGTDADCFNLSE
                     GNAYPIHLTTNQASVDISYLPIRTGTHNASLLVRDNLSRTDHLIPLIGLVIEPNPLGA
                     ISFSGGNENIVIASAPPLQTNTAITIETWFRWDVSGLIQFICSKAFEELEIHTTPVQG
                     LRFIPTYNIWLDSQPNILVPGTWQHIACVYDPASLLARIYVDGRDVTAGENGPGTIGA
                     PFQNTGQPFRLGIRASGDYPLNGALDETRIWDRALSQDEIRNLIHLRQEPGAPNLIAN
                     WRMNELNSERIWDQVNGLHGQMVNMEPQDRITSEVLIGTGTASTLTLDTPGQSYPFGD
                     TGITFIPDQLQAPGAVTVTHLDVLSGPDAFGGGSWVIRQYDSLSRSADLLCEVNADVS
                     EGLLPAGNFILKGRTPYWSPAWNTLLHASWADPDLDLLRFDALPLTAQHLSISWQRLA
                     LNIPQNLVIGRDATHVTLSWNGVAGATGYRVLSSDAPDGPFNPDLSGSFNASTWTAPL
                     GQARRFYRVQALAP"
     gene            complement(2644..4860)
                     /locus_tag="PLO35_08640"
     CDS             complement(2644..4860)
                     /locus_tag="PLO35_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79777.1"
                     /translation="MRCKLCCLLALLWVSLNGTPVARTILNTSVTHASASQNRCVTAC
                     YSGSVSTQLARITLWDTTNPVYPRQGVTINSNYTWGLYHFRQPTVRDSLLFYMDSFVL
                     RIVDIGNIDLPVTLSQLHVIYTQCFAVYDHYVLMGTTDGTILAFDISNPSQPVYVSTS
                     QAVGVINQMWVQGDYLAVNCGFPGNNRASIFAWTPATQNFTELVSVDAGSNITYVGCM
                     DERIVFQKDDGSVHFYSYGQGSGPTPVLQFAGGYNLRKIRTWRDVYYTFGEDNSIRIW
                     QLAPDNTRVQIGHYDLSHFSLDRGDLFELRDERLIYSVAESICIILDVSDLSAPPEFV
                     DRYVTGTDIASLGIPQDSDDIYFMNEDRLDHLQLSPMGLLSESPAIYNTGYGKRLQRW
                     NQFLYLVQGIQPNYSLSVYDLSDPASPELIWEQPFSSYSVFCVKGDFLYVGPNAAVQK
                     YHLEEDGLPSLEGTFSYTIPDNGELVYFLDMDNYAGCDYGIGVWGNILIGYYPILVYW
                     LPGGSTGFLNPPYLLYTANVVDDLLYLSGNGINILNLESGVPRLESTIDFNTHTKGLE
                     SSFLYEDRYLIECYSASNQIRVYDLLDPHHPQVLHIIYQSHSSKAMGMVGNRLVCANG
                     SYGIEVYDLPLEVETQDDQIPAPPELLAWPNPFRENVNLSFRLDKAGPLKLECYNLRG
                     QKVYTSRIEDAKTGDNHLNWDGRDQHGSPCAPGIYLIRIEGRDISLRKKITLLGRG"
BASE COUNT         1188 a         1478 c         1365 g         1196 t
ORIGIN      
        1 gatcacggct atgtcgagta ttctaccgat ggtggttaca actggtatat ctttccggcc
       61 tcctcatacc gcggtactgg tatctatgat accccgcttc agaacaatcc cgaggggccc
      121 tgttttgacc aggcgtccta tcctctctgg agcagctaca cagggtcagg ctttctaaac
      181 ggttatctgg tcacggatac ttttgacctc agtccctggg ctggctgtga ggatttcagg
      241 gtgggctacc gggccgtctg ggatggtgag tcgggtggga tgggctggta tatagatgat
      301 ttcagcatca gcatccaggc tccgggtgtg ccccatacac ccttgcctgt ttcggggacc
      361 accggttttg gcacctttga aaccatcaac tggacgggta tcaactccag ttcctatgat
      421 ttaactctcc acaccgaccc aggcttccca ggctttgtca ccctcagtgc aaacagctat
      481 ccctgcgtgg acctggcccc caacaccacc tatttctgga aagtgcgtgg ccacaacagc
      541 gtcgggactt ccgattggtc tcccgtttgg agctttacaa ccgggtctta tcgtagcacc
      601 aggcacaacg cttccagcct gtggatctcg gaggtgagtt ttggcagcat caacaactcc
      661 agcggctgga acggctatac ctactattcc ggcatcggga ccaacctcac aggcggttcc
      721 tccacaccca tacaggtctc tctggcagga ggttacggcc ccgaagccgt gggtgtgtgg
      781 atagacctcg accgcgatca cgtgtttggc tccactccgg cggagtttac ccagcttgcc
      841 tggaacggca gcggttttgg cggtaatata gtcataccac ccgctcagag tcccggaccc
      901 acccgaatga ggattcaggg catccataca gacaacaccg acgtgttgac tcccacaggt
      961 atcttcaact ttggcgagac ggaggattac gacctccagt tatataccgc tcccacactg
     1021 agtataagtc cttccagcgg agactttcca cccacgatgc aacactggca gtcatcgcct
     1081 atccgtttca ccttcagcaa catcggctcc ggaagcctgg atatcaccct caccgggatc
     1141 actggcacgg acgcggattg cttcaacctc tcggaaggca atgcctaccc catccacctc
     1201 accaccaatc aagcctcggt ggatatcagc tatctgccca tcaggacagg gactcacaac
     1261 gccagtctgc tggtgcggga caatctcagc cgcactgacc acctgattcc gctcatcggc
     1321 ctggtgatcg agcccaatcc cttgggggcc atatccttca gtggcggtaa cgagaatatc
     1381 gtgatcgcat cagctcctcc actgcagacc aacacggcca ttaccatcga gacctggttc
     1441 cgctgggacg tctccgggtt gattcagttc atttgttcca aagcctttga ggagctggag
     1501 atacacacta ctcccgtcca ggggctgcgc ttcattccca cctataatat ctggcttgat
     1561 tctcagccca acatcctggt cccgggcacc tggcagcaca tcgcctgcgt ctatgatccg
     1621 gcctcattgc tggccaggat ctatgtggac ggacgtgacg tcactgccgg agaaaacggt
     1681 cccggaacta tcggagcgcc cttccagaac accggacagc ccttcaggct gggaattcgc
     1741 gcttccggag attatccact gaacggagct ttggacgaga cccgcatctg ggaccgcgcc
     1801 ctcagccagg atgagatcag aaacctcatc cacctcaggc aggaacccgg agctcccaat
     1861 ctgatcgcca actggcgcat gaacgagctg aacagcgaaa ggatctggga ccaggtgaac
     1921 ggtctgcacg gtcagatggt gaatatggaa cctcaggaca ggatcacttc cgaggttctg
     1981 atcggcactg gaacggccag caccctcacc ctcgacactc ctggccagag ctatcctttt
     2041 ggcgataccg gcatcacttt tataccagac cagttacagg ccccgggagc cgtcactgta
     2101 acccatctgg acgtgctcag cggccccgat gcctttgggg gcgggtcctg ggtcatccgt
     2161 caatacgaca gcctttcccg ctccgctgat ctgctctgcg aggtcaatgc tgatgtgagt
     2221 gaaggtctgt tgcccgccgg caatttcatc ctcaagggca ggacacctta ctggagcccg
     2281 gcctggaaca ccttgctgca tgccagctgg gctgatccgg atttggattt gctccgtttt
     2341 gacgctttac cgctgacggc ccagcattta tccatcagct ggcagcgttt agccctcaat
     2401 atcccgcaaa accttgtcat tggcagggat gccacccatg tgaccctcag ctggaacggt
     2461 gtggcaggcg cgactggcta tcgggtcctg agctcggatg ctccggacgg acctttcaat
     2521 ccagacctca gcggcagctt caacgccagc acctggaccg ccccgcttgg acaggcaaga
     2581 agattctatc gcgtgcaggc cctggcccca taagcttgtc tcaatatgac ttagctgggg
     2641 agatcaaccc ctccccagca atgtgatctt tttgcgcaag ctgatatccc tgccctcgat
     2701 cctgatcaga taaatgcccg gcgcgcaggg tgatccatgc tggtcgcgac catcccagtt
     2761 caagtgatta tctccggttt tggcatcttc aatccggctg gtatacacct tttggccacg
     2821 gaggttgtag cattccagct ttagcggacc ggccttatcc aggcggaaac tgagattgac
     2881 gttttccctg aagggatttg gccaggccag cagttctggt ggcgcgggga tctgatcatc
     2941 ctgagtttct acctcgaggg gcaggtcata gacctcgatg ccgtaactgc cattggcaca
     3001 taccagtcgg ttgccgacca tgcccatggc cttgctgctg tggctttgat agatgatgtg
     3061 gaggacctgg ggatggtggg gatccagaag gtcataaacg cggatctggt ttgaggcgga
     3121 ataacactcg atcagatagc ggtcttcata aagaaaactg ctctcgagac ctttggtgtg
     3181 ggtgttgaaa tcgatagtgc tctccagacg gggaactcca ctctccaggt tgaggatatt
     3241 gatgccgtta ccgctcagat ataacaaatc atcaactaca ttggctgtat agagcagata
     3301 gggagggttc agaaaacctg ttgagccgcc aggcagccag taaaccagga tgggatagta
     3361 gcctatcagg atgtttcccc acactccgat cccatagtca cagcccgcgt agttgtccat
     3421 atccaggaaa tagaccagct ctccattatc cgggatagta tagctgaaag tgccttccag
     3481 actgggcagg ccatcttctt ccagatggta tttttgcacg gcggcattgg gcccaacata
     3541 gagaaaatct cccttgacgc aaaacacgga gtaagagctg aagggctgct cccagatcag
     3601 ttccgggctg gctggatcgc tgaggtcata gacgctgagg ctgtagttgg gctggatgcc
     3661 ctggaccaga tagaggaact ggttccatct ttgcaagcgc ttgccatagc cggtgttata
     3721 gatggcgggg ctttcgctga gcagacccat cgggctcagc tgcagatggt ccagccggtc
     3781 ttcattcatg aaatagatgt catcagaatc ctgagggatg cccagactgg cgatgtccgt
     3841 cccggtcaca tatctatcca caaattccgg aggagcgctg agatccgaga cgtccaggat
     3901 gatgcagata ctctcagcca ctgaatagat cagccgttcg tctctgagct caaacaaatc
     3961 cccccggtcc agcgagaaat gcgaaagatc gtagtgcccg atctggacgc gggtgttatc
     4021 aggggctaat tgccagatcc tgatgctgtt atcctctccg aaagtataat aaacgtcccg
     4081 ccaggtgcgg atctttctca ggttgtatcc acccgcgaac tgcaagacgg gagtggggcc
     4141 ggaaccctgt ccgtagctgt aaaagtgaac gctgccgtca tccttttgaa acacgatccg
     4201 ctcgtccatg cagcccacat aagtgatgtt gctgcccgcg tccacactca caagctcggt
     4261 gaagttctga gtggcgggag tccaggcaaa aatgctggcg cggttgtttc caggaaagcc
     4321 acagttcacc gccagatagt ctccctgcac ccacatttgg ttgatcactc caacggcctg
     4381 gctggtactt acatagactg gctgacttgg attggagata tcaaaggcca ggatcgtgcc
     4441 atcggtagtg cccatcaaga catagtggtc atacacggca aagcattgag tatagatgac
     4501 atgcaactgt gacaaggtga ccggaaggtc aatattgccg atatccacaa tccgcagcac
     4561 aaaggaatcc atgtagaaga gcaggctgtc ccgcactgtc ggctgccgaa agtgatagag
     4621 gccccaggtg taattgctgt tgatagtaac gccttgacgc ggatagaccg gattggtggt
     4681 atcccagaga gtgatgcgtg cgagttgggt ggaaacgctg ccgctgtaac atgccgtgac
     4741 gcaacggttc tgcgacgcgg aagcgtgtgt gacgctggta tttagaatgg tcctggctac
     4801 cggagtgcca ttcaaagaca cccagagtag agccagcagg cagcaaagtt tacaacgcat
     4861 gatcacctct attaaatgac atacaattat tgtgccagat ataatcaaaa atgctgcgcg
     4921 agaggcaatt ctgaacagat tgctgcgcat attctgagca taaagcctgt aacaccagct
     4981 cctttatgct gttcagctaa atccttatta acctttttaa aacgcaaaaa caatgaatct
     5041 tacaggtttc cccccgcctt cttgccgcat cctattacgg actcattacg gacttagtcc
     5101 gtaatgagtc cgtaatgaca ccgtaatgct aataaggagc acatcaacgc ttgtactcct
     5161 ccactctctg gctgtaaagg gtcagttgaa tttgcaaatt ggctcactga atattgcata
     5221 tggcttg
//
LOCUS       DAOSFN010000155         5214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_14123_length_5214_cov_8.796666, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000155 DAOSFN010000000
VERSION     DAOSFN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14123_length_5214_cov_8.796666"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /gene="purH"
                     /locus_tag="PLO35_08645"
     CDS             <1..795
                     /gene="purH"
                     /locus_tag="PLO35_08645"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425583.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOH79778.1"
                     /translation="YIDSSLRSVRLFEEPTVVITKHCNPCGIGSGFNLADAYRKAFAT
                     DTVSPFGGIVAVNRPLDMETAVSINRIFTEIIIAPAYEPGVLDVLIKKKDRRLIQYDP
                     EFLLKPRNPYEMKSMWWGYLVQEWDMVDIDISKWRVVTQKQPTQEQFDAMVWGWKVAS
                     LLRSNAIALTTHDQVMGLGIGQTSRIDSTAIAIWKAKKFNHDLSKAVCASDGFFPYRD
                     SIDELYQHGIKAVVQPGGSKSDAEVIQACNELDVAMVFTGFRHFRH"
     gene            846..939
                     /locus_tag="PLO35_08650"
     tRNA            846..939
                     /locus_tag="PLO35_08650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:881..883,aa:Ser,seq:gga)
     gene            981..2696
                     /gene="sppA"
                     /locus_tag="PLO35_08655"
     CDS             981..2696
                     /gene="sppA"
                     /locus_tag="PLO35_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOH79779.1"
                     /translation="MKTGKPILIGCLVFPLILIVAFGIGFCSSAGKLGSGVSVPSNAW
                     LVLDPAGLINDYNEIQSSGIFGTGITSAEELTARIKAASTDKRIKGMLIKPGFLEVSY
                     PNLGEISIAIAQFKGSQKPVIAYGTMMSQKDYLLCAMADSIWMEPSASAGLLLEGVSA
                     NLMFYKEALQKLGIRMHVMQSGEFKGAGEPYTRTELSAGTRANLEKALKARYDKLVGD
                     IATYRNIDSTRVIEVLENRDDIFISASMAKDYGLIDRAGSLDALKLQYGITKDNSISI
                     DDYSGKQPSAKAQKVAVVNLSGSIAAGPDYGSESMISASKVRKIIEAIEKDKTIKAVV
                     LRVNSPGGSALESELIYQQISQLKKKLPVVVSMGGVAASGGYYISCAGQYIYADPYTI
                     TGSIGVIMTLPETKELGTKLGLRSQTLRYGKFAGPINLFETYDPALLESFRRSSEGVY
                     TEFKQRVSESRQIPMDLMPEVAEGRIWSSDDALALKLIDAVGNLQDAIGKAADLAKLS
                     DYSVTNYPTRVSIYQALKESGIFQMLDRELKLRHGDPAEQIKDLLLSEFGPNQWLYRC
                     PYSLD"
     gene            2731..4248
                     /locus_tag="PLO35_08660"
     CDS             2731..4248
                     /locus_tag="PLO35_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOH79780.1"
                     /translation="MRKDSFNTEIIVDVHPLEKRVAVLEDNKLVELFVERKDRQNIVG
                     NIYKGHVKDVLPGMGAAFVEIGLERTAFLHYSDIVLDFLDVFENDKPRQRLNPNDSSQ
                     IGNLLKSGQEIVVQVHKGPIGSKGARLTGQISIPGKYLVLFPNKNKIAISRKIYNQGE
                     RSRIRNILSAVKDPNYGLIVRTEAEGCDEEDFKNEYNALAKTWRLTEKQIKYGKEPIC
                     VFEENALENYLIRDLFGEHVDRLVVNDKYFYKRILGYLEEVSPDLIPNVEFYKEDSPI
                     FDAWGIEKKIETVFHSRIYLPSGGNIKIEQTEALVAIDINTGSFTGKTHYEETIKRTN
                     IEAAAECARQIRLRDLSGVIIIDFIDMISENNKQEVLDTLKKGLRRDRAKNKVYSFTD
                     LGLVEVTRKRMRATLIANFSDPCPYCNGSGRVISRDAMTMRIHRWLSRAEYFTRNKKL
                     RVTVNPDLLSHIRAHEEYFIAFKDQIEFAPEAEMRTDSFKVQKLPGLEDITNKFN"
     gene            5132..5208
                     /locus_tag="PLO35_08665"
     tRNA            5132..5208
                     /locus_tag="PLO35_08665"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5166..5168,aa:Val,seq:cac)
BASE COUNT         1315 a         1494 c         1340 g         1065 t
ORIGIN      
        1 tatatcgaca gctctctgag atccgtgcgt ctctttgaag agcccaccgt ggtcatcacc
       61 aaacactgca acccctgtgg catcggcagc ggcttcaatc tggcagatgc ctaccgcaag
      121 gcctttgcca ccgataccgt ctctcccttt ggcgggatcg tggccgtcaa ccgtcccctg
      181 gatatggaaa cggccgtcag catcaaccgc atcttcaccg agatcatcat cgctcccgct
      241 tacgagcccg gagtgctgga cgtgctgatc aagaaaaagg accgccgtct gatccaatat
      301 gatccggagt ttttgctcaa accacgcaac ccctacgaaa tgaagtctat gtggtggggc
      361 tacctggtcc aggaatggga tatggtggat atcgacatct ccaaatggcg ggtggtcact
      421 cagaagcaac ccactcagga gcagttcgac gccatggtat ggggctggaa agtggcatcc
      481 ctgctgcgtt ccaacgccat cgcgctcacc actcacgacc aggtgatggg cctgggcatt
      541 gggcagacca gccgcatcga ctccaccgcc atcgctatct ggaaagcaaa aaaattcaac
      601 cacgatctca gcaaggccgt ctgtgcttcg gatggatttt tcccttaccg cgattccatc
      661 gacgagctct atcagcatgg catcaaagcg gtggtccagc caggcggttc aaagagcgac
      721 gcggaagtca tccaggcctg caatgagctg gatgtggcga tggtctttac gggcttcaga
      781 cacttccggc actaattaat gtttgacaga atcagccccc ggaaattttt ggagacactt
      841 ggcgcggaga tgtgtccgag ctggctgaag gagcacgatt ggaaatcgtg tgtatgtgca
      901 aaacgcgtac cctgggttcg aatcccagca tctccgccat ctgattctca cccccagaga
      961 aacccattta ggagcttcac atgaagacag gaaaacccat tctgatcggc tgtctggtat
     1021 tccccctcat cctgatcgta gcctttggta tcggcttttg cagcagcgcc ggcaagcttg
     1081 gcagcggtgt gagcgtgccc tcgaatgcct ggctggtact cgatcccgct ggcttgatca
     1141 acgattacaa cgagatccag agcagcggta tctttggcac aggcatcacc agtgctgagg
     1201 agctcacagc caggatcaaa gccgcctcga cagacaagcg gatcaaggga atgctgatca
     1261 aaccaggttt cctggaagta agctatccca acctgggtga gatcagtatc gccatcgccc
     1321 aattcaaagg cagccagaaa cccgtgatcg cgtatggcac gatgatgtcc caaaaggatt
     1381 atctgctctg cgccatggcg gacagcatct ggatggagcc ttccgcgtcg gcgggactgc
     1441 tgctggaagg cgtctcggcc aacctgatgt tttacaagga agccctgcag aagctgggga
     1501 tcaggatgca cgtgatgcag tccggagagt ttaaaggagc gggtgaacct tacacgcgga
     1561 cagaattgag cgccggcacc agagccaacc tcgaaaaagc tctcaaggca cgttacgaca
     1621 agcttgtagg cgatattgcc acctatcgca acatcgacag cacccgcgtg atcgaagtgc
     1681 tggaaaatcg cgacgacatc tttatctctg cttccatggc taaagactat ggtctcatcg
     1741 accgggccgg atctctggac gcgctgaagc ttcaatatgg gatcaccaaa gacaacagca
     1801 tttccatcga tgactatagc ggcaaacagc cctccgccaa ggcccaaaaa gtagcggtgg
     1861 tcaacctcag cggcagcatc gctgccggtc ccgattatgg ctccgaatcc atgatttctg
     1921 cctcgaaagt ccgcaagatc atcgaagcca tcgaaaagga caaaaccatc aaagcggtgg
     1981 tcctgagagt caacagccct ggtggcagcg ctttggaatc cgagctcatc tatcagcaga
     2041 tcagccagct caaaaagaaa ctgcccgtgg tggtttccat gggcggagtg gcagcctcag
     2101 ggggttatta catctcctgc gccggccagt atatttatgc cgatccctac accattaccg
     2161 gctcgatcgg agtgatcatg accctgccgg agaccaagga actgggcacc aagctgggcc
     2221 tgcgcagtca aactctccgt tatggcaaat ttgccggtcc catcaacctt tttgaaacat
     2281 acgatcccgc tctgctggaa tctttccggc gcagttcaga aggggtctat acagaattta
     2341 aacagcgcgt ctcggaatca cgccagatcc cgatggacct gatgccggaa gtggctgaag
     2401 gccggatctg gtctagcgac gacgctcttg ccctcaagct gatcgacgcg gtgggaaacc
     2461 ttcaggatgc catcggtaaa gcggcagacc tggccaagct tagcgattac agcgtgacca
     2521 actaccccac ccgtgtctcg atctatcagg ccctcaagga aagcggcatt ttccagatgc
     2581 tggaccgtga gctcaagcta cgccacggag accccgctga gcagatcaaa gacttgctgc
     2641 tcagtgagtt cggccccaac cagtggctct accgctgccc ctatagcctg gattgaggag
     2701 cgctaaccaa ttttataccc ataggaaatt atgagaaaag acagttttaa caccgagatc
     2761 atcgtggacg tacacccgct cgaaaagcgc gtcgcggtgc tcgaagacaa caagctggtg
     2821 gagctctttg tggagcgcaa agaccgccaa aacatcgtgg gaaacatcta taaaggccac
     2881 gtcaaagacg tcctgcccgg aatgggggcc gcctttgtgg aaattggcct ggaacgcacc
     2941 gccttccttc actactccga tatcgtgctg gactttctgg atgtattcga aaacgataaa
     3001 ccccgccagc gtctcaatcc caacgactcc tcccagatcg gcaacctgct caagtccggt
     3061 caggagattg tggtgcaggt ccataaaggt cccatcggct ccaaaggggc caggctgaca
     3121 ggccagatat ccatacccgg caagtatttg gtgctcttcc ccaataaaaa caagatcgcc
     3181 atctcccgca agatctacaa ccagggagaa cgcagccgca tccgcaacat cctctcggcc
     3241 gtcaaggacc ccaactatgg cctgatcgtc cgcaccgaag cagagggctg cgacgaagaa
     3301 gacttcaaga acgaatacaa cgccctggcc aagacctggc gcctcaccga aaagcagatc
     3361 aaatacggca aggagccgat ctgcgtgttc gaagagaacg ccctggaaaa ctacctcatc
     3421 cgcgacctct tcggtgagca cgtggaccgc ctggtggtga acgacaagta tttctacaag
     3481 cgcattttgg gctatctgga agaagtatct ccggacctca ttcccaacgt ggaattttat
     3541 aaggaagaca gccccatttt cgacgcctgg ggtatcgaaa agaagatcga gacggtgttt
     3601 cacagccgca tctatttgcc cagcggcggc aatatcaaga tcgagcagac cgaggccctc
     3661 gtagccatcg atatcaacac cggcagcttt accggcaaga cccactacga ggagaccatc
     3721 aaacgcacca acatcgaggc ggccgctgaa tgcgcacgcc agatcaggct gcgcgacctc
     3781 agcggcgtga tcatcatcga ctttatcgat atgatcagtg aaaacaacaa acaggaagtg
     3841 ctggacaccc tcaaaaaagg tctcaggcgc gaccgcgcta aaaacaaggt ctatagcttc
     3901 accgatctgg ggctggtgga agtgacccgt aaacgcatgc gcgccaccct catcgccaat
     3961 ttctcagacc cctgccccta ctgcaacggt tccggcagag tgatatcccg ggacgccatg
     4021 acgatgcgca ttcatcgctg gctgagccgc gcggaatact tcacccgcaa caagaagctc
     4081 cgtgtgaccg tcaatccgga cctgctctct catatccgcg cccacgagga atactttatc
     4141 gccttcaaag accagatcga gtttgccccc gaagccgaaa tgcgcaccga cagcttcaaa
     4201 gtgcagaagc tgcccgggct ggaagatatc accaacaagt tcaactgagt ttaggacact
     4261 cccgattggt attacgggtg cattacgggt gcgacccgca atgcacccgc aatgcacccg
     4321 taatggaaag cggcaggaaa ctccgctttt ttaagcattg aataataggc atcactggct
     4381 tcagagctgg tgcgaaaact ggcgaagcca gaaactgata cagccccgtt ggagttcaag
     4441 cggccacgat aaaggtatgc atgacaacga gtttgcgggc cgcttggact tcaaccagca
     4501 cactcgcgag aaaaaccccc tccgatgtac tgcacgtaag tccaaagccc cgtcaggtat
     4561 agatataaac aaataataca ttttagggct ttaaactcca agatggcatt tccgcacaag
     4621 ctctaaagcc agtgatccgc ttatgtgcaa cggtctctaa gagacagcgg cttctgatgt
     4681 ggcctaataa tctgaaatac cactcctgac ggggatcagt ccaatgtgtg gtacgcgtaa
     4741 tctatctgaa atagcgtccc tgacgggact caatacgcca ttttcgcaca agctcttcag
     4801 ccggttatag taccggtctt cagccggcac ccacagcact ctctggaagg gcaaggacaa
     4861 cagcggccgg gctgtctcag ggggagttta cctctacagg atgacggccg gcgagtatag
     4921 cgcttcccgc aaaatggtcc tgatgaagtg acccggtccc gggctatctg tttgcctcat
     4981 agctcattac cggcaatgaa gccaggggct gatcgcttgt gggctgggat accccagcat
     5041 agggtgacag cctttacagg cctcgtctca agccttagtc cgcagatttc tattgacaaa
     5101 tagctacctt tttgcagaat gacttcaccg tgggtgatta gctcagttgg ttagagcgct
     5161 acgttcacat cgtagaggtc actggttcga atccagtatc acccaccatt tttt
//
LOCUS       DAOSFN010000156         5187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_14226_length_5187_cov_7.764030, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000156 DAOSFN010000000
VERSION     DAOSFN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14226_length_5187_cov_7.764030"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="PLO35_08670"
     CDS             complement(<1..1795)
                     /locus_tag="PLO35_08670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOH79781.1"
                     /translation="MARRIPMEDFFRNPEKCYVMVSPRGHYLSWMEPWERRLNIYVKN
                     LQSGEVVRVTSVTERDVQSYFWANDQRIIYAMDKDGDENVRLIAVDWDGGNHIDLTPL
                     ENVKCMLLDELINDDEHILFGMNQRNPELFDVYRLNVNSGEMEIVAENWGDITDWLCD
                     HDGKLRLAISSDGVNNGVFYRPTEADEWKEIAVYNFRESFAPLFFTFDNRALYASSNV
                     GRDKAAIVEYNLDNQTENKVIFEHPEVDVNTLIRSTKRKVITGVYFETDRGQYQLFDD
                     HRKRIQEFVDAKLPLYQNVVVNSDLDESIYIIYSSNDRTYGSYYWLDVRAWKLNKLFD
                     LAPWLHEEELAEMKSIQYSARDGLPIKGYLSLPPGVEPSKLPLVVNPHGGPWARDSWG
                     FNPEVQFLVNRGYAVLQMNFRGSTGYGRQFWEASFKQWGLSMQDDITDGVQWMIDQGI
                     ADPTRIAIYGGSYGGYATLMGIVKTPDLYAAAVDYVGVANLFTFMDSFPAYWKPIVDM
                     MHEMIGHPDQDAELYKAVSPVMHVDRIKTPLFIAQGANDPRVKQHESDQMVEALRQRG
                     IAVEYMVKENEGHGFANEENSFDFYNAMEAFL"
     gene            2142..2396
                     /locus_tag="PLO35_08675"
     CDS             2142..2396
                     /locus_tag="PLO35_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79782.1"
                     /translation="MKDRFTLYQIFSGLIFGGCTVLFCVAVALSGGFEPPIFVLFALA
                     GFAIAGVLAHLLIEQARDIRRMAEDLRAVFELLKHHKTEL"
     gene            complement(2406..4163)
                     /locus_tag="PLO35_08680"
     CDS             complement(2406..4163)
                     /locus_tag="PLO35_08680"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HOH79783.1"
                     /translation="MKERKGLCLIALCLLILLPLGADIFQGGSGTANDPWLVATAEQL
                     NLVRNYLNNFNHYFKQIADIDLGVDPYNLGQGWVPIGSYQNNSFRGSYDGDGFSISGL
                     YINSSTFPHQGLFGYAYGARLSNVHVVNANVTGSYAVGALLGLGKESTVISGCSSSGS
                     VTGAQYSVGGLVGTLEWWSSMSASHSSVSVTGMQSTVGGLLGNSYYVSLISGCSSTGP
                     VNCPDTNSSGSAGGMIGSSGYSTIRDSWSSSVITALSNSSVGGFIGSVTGSNLIEDCY
                     ASGNVTARNQCGGFVGYNYQGSTIHLCYAIGNVTAVEMYEGYCGGFVGTNQSGGEGLA
                     IIYRCYSTGIANAYKSTGGFVGRNVSGKVYKCFATGNAIGTSRIGSFCGESLSNGEIS
                     DCYASGSVSGNEQSGGFIGSNQEPSLLARCFNYGPVSFSGTYYVGGFCGIGAASQATD
                     CYWNTQTTGISTSACGSGRSTAEMTWPYDPNTYQNYDFTSIWGQDQGYVFNNGYPYMS
                     WRYLIPPGALTVSQTGSTIELSWTSVPGANSYKVYASSDPYADDDQWAQLGTTASLAY
                     TTSAESKMFFRVLASSSAP"
     gene            4397..5167
                     /locus_tag="PLO35_08685"
     CDS             4397..5167
                     /locus_tag="PLO35_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOH79784.1"
                     /translation="MDRPQVLLYVCASVDGRITMAPGATMFDMYKQPELYAMLCAPEE
                     WQSFSKAVFDLYKPDMFLEGSHMVVAEKAELTELPGFEGDPEDLYGDYLPEDILRRPG
                     RKTWTAVVDGRGRFRNGYTADCDDPATYMVHLTTQTAPPEYLAFLRQRHIPYLIQGQD
                     RVDLPRMLEKVKAKLGVRTIATSSGGRLSGALIRAGLLDEINILLSPIVIGGYEIPSL
                     FSSPGPEWPTLPPDKLQLVDVTTLPHGKLWLRYKVLGG"
BASE COUNT         1150 a         1457 c         1377 g         1203 t
ORIGIN      
        1 tcaggaaggc ctccatggcg ttgtagaaat cgaagctgtt ttcctcattg gcaaagccgt
       61 ggccctcgtt ttccttgacc atatactcca ccgcgatgcc tctctggcgc agggcttcca
      121 ccatctgatc ggattcgtgc tgcttgactc tgggatcgtt ggcaccctgg gctataaaga
      181 gcggcgtctt gatccggtcc acgtgcatga ccggcgagac ggctttatag agctcggcgt
      241 cctggtccgg gtgaccgatc atctcgtgca tcatgtccac gatcggtttc caataggcag
      301 ggaaggaatc cataaaggta aagaggttgg ccactcccac ataatccacc gccgcggcat
      361 agagatcggg cgtcttgacg atacccatca gggtggcgta acctccatag ctgccgccat
      421 atatggcaat gcgggtggga tcggcgatgc cctgatcgat catccactgc accccgtccg
      481 tgatatcgtc ctgcatgctc agcccccact gcttgaaaga agcctcccaa aactggcgtc
      541 catagcccgt cgagcctcgg aagttcatct gcaggacggc atagccacgg ttgacgagga
      601 actggacttc cggattgaat ccccaggaat cgcgtgccca gggacccccg tgaggattga
      661 ccaccagggg cagcttgctg ggttccactc cgggcggcaa actaaggtat cccttgatcg
      721 gcagaccgtc tctggcgctg tattggatgg atttcatctc ggccagttct tcttcatgca
      781 gccagggggc caggtcaaag agcttgttca gtttccaggc gcgcacgtcc agccagtagt
      841 agctgccata agttctgtca tttgaggaat atatgatata gatactctca tcgaggtcgg
      901 aattcaccac cacattctgg tagagcggca gcttggcatc cacaaattcc tgaatgcgct
      961 tgcggtgatc gtcgaagagc tgatactggc cccggtccgt ctcaaaatac actccggtga
     1021 tcactttgcg cttggtggac cggatcagcg tgttgacgtc cacctcggga tgttcgaaga
     1081 tcactttgtt ttcggtctgg ttgtccagat tgtattccac gatcgccgct ttatccctgc
     1141 ccacattgga tgaggcatac aaggcgcggt tgtcaaaggt gaaaaacaga ggggcgaaac
     1201 tctcccggaa gttatagacg gcaatctcct tccactcgtc agcttccgtg ggccggtaaa
     1261 aaacgccgtt gttgaccccg tctgaagaaa tggccagacg cagcttgccg tcgtgatcgc
     1321 agagccaatc cgtgatgtca ccccagtttt cagctacaat ctccatctca ccgctgttga
     1381 cgttgagacg gtaaacgtca aagagctctg gattgcgctg gttcatgccg aagaggatgt
     1441 gttcgtcatc gttgatcaat tcatccagca gcatacattt gacgttttcc agaggggtga
     1501 gatcgatgtg attgccgcca tcccaatcga ccgcgatcag cctcacgttt tcgtcaccgt
     1561 ctttatccat agcgtagatg atccgctggt cgttggccca gaaatagctc tggacgtccc
     1621 gttccgtgac gctggtgact ctgactacct caccgctttg caggttcttg acgtagatgt
     1681 ttagccttct ttcccaaggt tccatccagg atagataatg cccccggggc gagaccatga
     1741 cgtagcactt ttcaggattg cgaaagaaat cctccatggg aatccttctg gccattgttc
     1801 ctcctacttg tgtgaaaata tctttttacc gctgcggggg ttctgagttt ttgtcaatgt
     1861 atttctttga gggtgagata cctgcgatag ctgagatgct atgcatgtga gagcactgcg
     1921 agctggcgag attgcgtggc gagtttccta aaacccaaaa ccctacaccc cttccagaca
     1981 gccgggacga ggttgcatga caccatgcaa aaccgcccaa tcgcttctct catcggaggc
     2041 tcccagatat cgcttgacca caagagctct tccgggaccc ttgcgctcgc gtccaaacct
     2101 tttccgaggc tggatagcct ggaaatccca gggagaaatc catgaaagac cgtttcaccc
     2161 tctatcagat cttctcaggc ctcatctttg gaggctgcac ggtgctgttc tgcgtggccg
     2221 ttgccttgtc cggaggcttt gagcctccca tctttgtgct cttcgcttta gccggatttg
     2281 ccattgcggg agtcctggcc catctcctga tcgaacaggc cagggacatc cgcagaatgg
     2341 ctgaggacct gagggcggtg ttcgaactgc tcaagcatca caaaaccgag ctctgagcca
     2401 gctctttagg gcgcggacga tgaagccagc accctgaaaa acatcttgga ttcagcgctg
     2461 gtggtataag caagagacgc cgtcgtgcca agctgggccc attgatcatc atcagcgtag
     2521 ggatcggaac tggcatagac tttataggaa ttcgcgcccg gcactgaggt ccagctaagc
     2581 tcgatagttg accccgtttg gctgacagtc agagctccgg gagggatgag atagcgccag
     2641 ctcatgtagg gataaccatt attgaacaca tagccctgat cctgtcccca gatgcttgta
     2701 aagtcataat tctggtaggt attgggatca tagggccagg tcatctcagc ggtgctgcgt
     2761 ccactgccgc aggcactggt ggaaatgccg gtggtctggg tattccaata gcagtcggta
     2821 gcctgagaag cagctccgat gccgcaaaat cctcccacat aataggttcc gctgaaactg
     2881 acggggccat agttaaaaca tctggccagc aggctgggct cctggttgct gccaatgaag
     2941 ccgcccgatt gttcattacc agacacactt ccgctggcgt agcaatctga tatctcgccg
     3001 ttgctgaggc tttctccgca aaaactccct atccggctgg ttccgatagc attgcctgtg
     3061 gcaaaacatt tatagacttt cccgctcaca ttgcgtccca caaagcctcc cgtgctcttg
     3121 tacgcgttgg cgatgccggt gctgtaacat ctatagatga tggcgagtcc ctcacctccg
     3181 ctttgattgg tgcccacaaa gcctccgcaa tagccctcat acatctccac ggcggtaaca
     3241 tttccgatgg catagcacaa atggatggtg gagccctgat aattgtagcc tacaaagccg
     3301 ccgcactggt ttctggctgt cacattgccg ctggcatagc aatcctcgat cagattgctg
     3361 cccgttaccg agccgataaa tcctcccacc gagctgtttg agagggcggt gatcacggaa
     3421 ctgctccagg agtctctgat cgtcgagtag cccgagctgc caatcatgcc tcccgcgctg
     3481 ccggaggaat tggtgtcagg acaattgacc ggtccggtgg aagaacagcc tgagatcagg
     3541 ctcacgtaat agctgttgcc cagcagcccg cccaccgtcg attgcatccc ggtgacgctg
     3601 accgagctgt ggctggcaga catcgaagac caccattcca gcgtgccgac cagaccgccc
     3661 acgctgtatt gcgcgccggt tacagatccg gatgaagaac agccgcttat gacggtggat
     3721 tcctttccca ggcccaacag cgcacccacc gcgtaggaac ctgtcacatt cgcgttcacc
     3781 acgtgcacat tgctgagcct ggcgccatag gcatagccaa acaagccctg atgggggaag
     3841 gtggaactgt tgatatagag cccgctgatg ctgaaaccat ctccatcata gctgccccga
     3901 aagctgttgt tttggtagga tccgatcggc acccagccct ggccaagatt gtagggatct
     3961 actccgagat caatatcggc gatctgtttg aagtaatgat tgaagttgtt cagatagttt
     4021 ctcaccagat tcagttgctc cgccgtggct accagccagg ggtcgttggc cgtgccggac
     4081 ccgccttgaa aaatgtcagc gccaagcggc agcagaataa gcaaacacaa agctatcagg
     4141 cataagcctt ttctctcttt catcgtttcc tccagctttt atctctggtc tggataataa
     4201 aactattccc gctatttagt caagcatttt atcaatttcc ggatgcgggg atggccatcc
     4261 agatctatgc cattttacct gatgctgatg ccgcagaggc attcgccggt gcttaaagaa
     4321 gtgattgaca gcatcagccc ggccaggaaa agggtgtgca acccgcaaaa acagaccaga
     4381 tcaaggggag gagataatgg acagacccca agtgctgcta tatgtctgcg cctcagtgga
     4441 tggcagaatc accatggccc ccggagcgac catgtttgac atgtataaac agcctgagct
     4501 ctatgccatg ctgtgcgctc cggaagaatg gcagagcttt tccaaagcgg tatttgatct
     4561 ttataaaccc gatatgtttc tggaaggcag ccacatggtg gtggccgaga aagcggaact
     4621 gactgagctg ccaggctttg agggtgatcc tgaggatctg tatggggatt atctgccgga
     4681 agacatcctc aggcgtcccg gccgcaaaac ctggaccgcc gtggtcgatg gcagaggcag
     4741 attcagaaac ggttacacgg ccgattgcga cgatccggcc acctatatgg tgcatctgac
     4801 cacccaaaca gccccgcctg agtatctggc ctttttgcgc cagcgccaca ttccctatct
     4861 gatccaggga caagacagag tcgatctgcc ccggatgctg gaaaaagtga aggccaaact
     4921 gggcgtgcgc accatcgcca ccagttccgg aggacggcta agcggcgctc tgatcagagc
     4981 tgggctgctg gacgagatca atatcctgct cagccccatc gtgatcggcg gctacgaaat
     5041 ccccagcctg ttttcctctc ccggaccgga atggcccacc ctcccgccag ataagctcca
     5101 gcttgtggat gtgaccaccc tcccacacgg caagctgtgg ctgaggtata aggtgttagg
     5161 gggttaagcg ttacgcgtga agcgtta
//
LOCUS       DAOSFN010000157         5060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_14723_length_5060_cov_8.637962, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000157 DAOSFN010000000
VERSION     DAOSFN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14723_length_5060_cov_8.637962"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..956)
                     /locus_tag="PLO35_08690"
     CDS             complement(159..956)
                     /locus_tag="PLO35_08690"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOH79785.1"
                     /translation="MASDLSFTLTQNQYSDNWAGSELSNVTWVANSNSSAEKQMAKWL
                     NNKTTLKLAFGQTHLQKRDIAGDVTWDHPTKSTDKIDLESLSKFTLQAWVDPYLSLRA
                     ESQFIDESDATLTRIVNPILLTESAGVMKTLWKKERDELSLRLGAAFREKLDREVLQL
                     SGEREMVTTVDGGAEFVSEFKHIFLLMDANFKSKFWAYQAVFNSKSDELNDDWKTVDF
                     VWENVLTAKLWKIVSASLTFEMRYDKEQIDAVQWKQILGLGVSYSLF"
     gene            complement(1039..1815)
                     /locus_tag="PLO35_08695"
     CDS             complement(1039..1815)
                     /locus_tag="PLO35_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOH79786.1"
                     /translation="MDFNALMEKVPAFLATNGLKLIAAILIYLIGKWIASALTKVFRK
                     VLIKSKMDDTLVSFLGNIVYALLLIFVIIAAIGKLGVQTTSLVAIIGAAGLAVGLALQ
                     GSLSNFASGVMLILFKPFKVGDTVIAAGTNGTVHDIGIFHTVIVTSDNKKVIIPNTPI
                     AGGAITNFSAMPTRRIELTVAVPGTVDMNQVRDILKGLMDADARILKDPAAAIVITEA
                     TPGEIKYGLFPHVKNADMGAVQADLTEKIKLSLTANGIWA"
     assembly_gap    2075..2174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2242..>3598)
                     /locus_tag="PLO35_08700"
     CDS             complement(2242..>3598)
                     /locus_tag="PLO35_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOH79787.1"
                     /translation="SSLVSGTVSGSGGQAIAGASVVLNNGSHSTVTDNSGYYQFYGLV
                     PGTYIIDIEAFGYFPGNQSFELTVNQELSINVSLIPLPVVNVTGIVMGSDTGAPIQGA
                     TVSLSGYANFAGVTDVSGAFLISGVYAAMAYDCLIIAPGYNTWSGPIALSYSDFNLGT
                     ITLSEYAYPPSQVNAVLNDGGTAVLVSWGPPSLENRDLLGYKVWRLPNGQELNPAVWT
                     ELTSQPLQGFSFTDNGWISIPNGYYRWAVRSWYGAEVYSEPALSDTLQNLVVNGILAG
                     TVRQTNTGYIAGATVSLAGTSTTTNQSGVYQVIVPVGVHSVTASAPGFISQTVDNVSI
                     LEGQVSSLHFVLEVDTSPSDDPVLPVWTSLRGNYPNPFNPSTTIAFSLGIQGRVRLEI
                     YNLKGQLIRVLLDDIKPVGMHQIVFDGLDRHGSPLGNGVYVLKMLAGKEVFSSRMLLL
                     K"
     assembly_gap    3599..3698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3699..4720)
                     /locus_tag="PLO35_08705"
     CDS             complement(<3699..4720)
                     /locus_tag="PLO35_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79788.1"
                     /translation="MKHLVLTALLVALALSCQNVYADNYKSGNGLRDIVTLPYTQNFD
                     GVTAPAMPAGWQVMVSPAGSDAIVNTDSSDAHSAPNSVRFHGGYNGGATLYLIAPLLA
                     DSIAVQSVRMRLWLKAISAYQLQVGVMDDPANPASFTLVNTLTFPGSWYELTQSLAAY
                     SGTGRYIALRTVNLTIGGSLLLDDFRLERIWPVDLAAIGLDTDTTQSLQAGHPSSFEI
                     TVQNLGANLQDIYYVVLCNAINDMQLATANGYNLQPGQTWNVDLTWTPVEAGSFTLYA
                     KVVLTGDGDLSNNHSPNLTIEVSPAGQESVTIGSGNQLARIPMDFYWHNSLYENIYYA
                     SELQGS"
BASE COUNT         1016 a         1286 c         1399 g         1159 t
ORIGIN      
        1 tctcatgttc tcatgtctca ttgtaagata gaccccctgt tttctccgct ttctccgctt
       61 tctctgaggt ttgttgtcgt taagcttcac gcgctgcggg caaaacaaaa gcgcggcacc
      121 cagattgggc accgcgcctt attcatgaca ggatgagttt aaaacagtga gtagctcact
      181 cccaggccca ggatctgttt ccactggacc gcgtcgatct gctctttgtc gtagcgcatc
      241 tcgaaggtga ggctggcgct cacgatcttc cagagcttgg cggtgagcac gttttcccac
      301 acgaagtcga cggttttcca atcgtcgttg agctcgtcgc tcttggaatt gaagacggcc
      361 tgataggccc agaatttgga tttgaagttg gcatccatca gcaggaagat gtgcttgaat
      421 tcactcacga actccgcacc gccgtccacg gtggtcacca tttcacgctc tccggaaagc
      481 tgcagcactt cccggtccag cttttcacgg aaggcggcac cgagacggag gctgagctcg
      541 tcgcgttcct ttttccagag ggtcttcatc acaccggcgg attctgtgag caggatggga
      601 ttgacgatgc gggtgagggt ggcatcggat tcgtcgatga actgggattc cgctctcagg
      661 ctgagatagg ggtccaccca ggcctgcagg gtgaatttgg agagggattc gaggtcgatc
      721 ttatcggtgc ttttggtggg gtggtcccag gttacgtcac cagcaatgtc gcgtttttgc
      781 aggtgagtct gaccgaaggc gagcttgagg gtggtcttgt tgttcagcca tttagccatc
      841 tgcttttcgg cggatgaatt ggagtttgcc acccaggtga cgttgctgag ctcggagccg
      901 gcccagttgt cgctatattg gttttgggtg agggtaaagc tgaggtcgct ggccagtttc
      961 cagccttagg catggctgag gctgaggacg gccaggagga acaggatcag gctgaatttc
     1021 ttcatagctg ccagtccttt aagcccagat gccgttagcg gtgaggctga gcttgatctt
     1081 ttcggtgagg tcggcctgga cggcacccat atcggcgttt ttaacgtggg ggaagaggcc
     1141 atatttgatc tcgccagggg tggcttccgt gatcacgatc gcggcggcgg ggtctttgag
     1201 gatgcgggca tcggcatcca tgaggccttt gagaatgtct ctcacctggt tcatgtccac
     1261 cgtgccggga acggctaccg tgagctcgat cctgcgggtg ggcatggcac tgaagttcgt
     1321 gatcgctcca ccggcgatgg gagtgttggg aataatgacc ttcttgttgt ccgaggtcac
     1381 gatcacggtg tggaagatgc cgatgtcgtg gacggtgccg ttggtgccgg ccgcgatgac
     1441 ggtgtcacct accttgaacg gtttgaagag gataagcatg actccggagg caaagttgct
     1501 gagtgagccc tgcaaggcca ggccaaccgc cagtccggcg gctccgatga tcgctaccag
     1561 agaggtggtc tgcacaccca gtttgccgat ggcggcaatg atcacaaaga tcagcagcag
     1621 ggcatagacg atattgccta gaaaactgac aagggtgtcg tccatcttgc ttttgatgag
     1681 caccttacgg aagaccttag tgagggcgct ggcaatccat ttaccgatca ggtagatcag
     1741 gatcgcggcg atgagtttga ggccattggt ggccaaaaaa gcgggaactt tctccatcag
     1801 agcgttaaag tccattctta actccttttt atatccaaaa atatcccgtc ccggcctgag
     1861 ctcttccaca agctggaacg ctcacgctta acgggtgtga ctatatatgt aaaataatcg
     1921 gcattcctgt tttctccaaa tacttttgat atagcgattt ttttgatgcc gaaccatgcg
     1981 gctgacctca aagcgaggca tgccgcacat ctatccggag ccaggaaaag ttattgatcc
     2041 cggtgtctat ttgctcgctt ttatcaggga tttgnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnntttttt tatagaaacg cggtctgagc caggatgtca atcctttttt
     2221 tcccggttct gcctgggaag tctatttcag cagcagcatc ctggagctga agacctcttt
     2281 acctgccagc attttaagca catagacccc gttacccagg ggtgatccat gccggtccaa
     2341 tccatcaaag acgatttgat gcatccccac aggcttgata tcatccagca gcactcggat
     2401 caattggcct ttcaggttat agatttccag gcgtaccctg ccctgaatgc ccaaagaaaa
     2461 ggcaatcgtg gtgctgggat tgaagggatt gggatagttt ccccgcaggg atgtccagac
     2521 aggcagcacc ggatcatccg aaggggaggt gtctacctcg agcacaaagt ggagagagct
     2581 cacctgtccc tccagaatgc tcacgttgtc caccgtctga ctgatgaaac ccggcgcgga
     2641 agcggtgacg ctgtgaactc ccaccggcac aatgacctgg tagactcccg actggttggt
     2701 ggtggtgctg gtgccagcca ggctgacagt ggccccggca atatagccgg tgttggtctg
     2761 tctcacggtc ccggccagaa tcccgtttac caccaggttt tgcagagtat cagaaagggc
     2821 cggctccgaa taaacctcag cgccatacca ggacctcacg gcccagcggt agtatccatt
     2881 ggggatggaa atccagccgt tgtccgtgaa gctgaagccc tgcaagggct gagatgtgag
     2941 ttctgtccac acggctggat tgagctcctg gccgttgggc aagcgccaca ccttatagcc
     3001 cagcagatcg cggttttcca ggctgggagg gccccatgac accaggacgg cggtcccgcc
     3061 atcattcaaa acggcgttta cctggctggg cggataggca tactcgctga gggtgatggt
     3121 gcccaggttg aaatcactgt agctcagtgc tatgggtcca ctccaggtgt tgtaaccggg
     3181 agctatgatc agacagtcat aggccatcgc ggcatagacc ccggagatca ggaaagcacc
     3241 gctgacgtcg gtcactccag cgaaattggc atatccgctc agagagacgg tcgctccctg
     3301 gatcggagct ccggtgtcgc tgcccatcac gattccggtc acattgacca ccgggagggg
     3361 aatgaggctc acgttgatgc tcagctcctg gttgacggtc agctcaaagc tctgattgcc
     3421 gggaaagtag ccaaacgcct cgatatcaat gatataggtg ccgggaacca ggccatagaa
     3481 ctgataatag ccgctattat ccgtgacggt gctgtggctg ccgttgttga gcacaacgct
     3541 cgctccggca atggcttggc cgccactacc actcaccgtt ccactcacca gactgctgnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gctgccttgc agctctgagg
     3721 cataatagat gttttcatac agggagttgt gccagtagaa atccatcggt atcctggcca
     3781 gttgattgcc ggaaccgatc gtgacgcttt cctgtcccgc cgggcttacc tcgatcgtga
     3841 ggttgggact atggttgttg ctcagatcgc cgtctccggt cagcaccact ttggcataga
     3901 gagtgaaaga ccctgcttcc acgggggtcc aggtcagatc aacattccag gtttgccccg
     3961 gctggaggtt gtagccgtta gcggtggcca gttgcatatc attaatggcg ttgcagagca
     4021 ctacgtaata gatgtcctgg aggttggccc ccaggttttg gaccgttatc tcaaaactgg
     4081 atggatggcc cgcctggagg ctttgagtgg tgtccgtatc caggcctatc gctgccaggt
     4141 ccacgggcca gatccgctcc agccgaaagt catccagcaa gagcgagcct ccaatcgtca
     4201 ggttcacggt tcgcagggca atataacgtc cggttccgga gtaggcagcc aggctttggg
     4261 tgagttcata ccagcttccg ggaaaggtga gggtattgac cagcgtgaaa ctggccggat
     4321 tggcaggatc gtccatcacg ccgacctgta gttggtaagc gctgatcgcc ttgagccaca
     4381 acctcatcct tacgctttgg accgctatgc tgtccgccag caggggagcg atcagataca
     4441 gagtggcgcc gccattgtac ccgccatgaa aacggacgct gttgggagcg ctgtgagcgt
     4501 cactgctgtc cgtgttcacg atggcatcag accccgcggg gctaaccatc acctgccatc
     4561 cggctggcat ggccggggct gtgactccat caaagttttg ggtatatgga agggtgacga
     4621 tgtcacgtag gccgttgcca cttttatagt tgtccgcata gacgttctgg catgaaagtg
     4681 ccagggccac cagcagggct gtgaggacta agtgcttcat ctataactcc ttggctacaa
     4741 tgatgatccg gataatcgga ggccggcagg gatgtccgcg ctcctcatag gagcttgggg
     4801 cccggtgccg cacgtttatt tcagggccgc atttttgtaa agaaatattt gttccaaagc
     4861 cacagaaacc tgtgaataac accggtcgca ggataggctt caggcccgct gagggtagat
     4921 tccaccccac tgcccaaacc cgcattctct catcagtcag gctttcaagg gctttgcctg
     4981 tatgcttaaa agggttactc tcccatttcc acttccactg gcagtgacag attctccccc
     5041 cttagtattg cgggatcatt
//
LOCUS       DAOSFN010000158         4961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_15111_length_4961_cov_8.405218, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000158 DAOSFN010000000
VERSION     DAOSFN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15111_length_4961_cov_8.405218"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(545..2389)
                     /locus_tag="PLO35_08710"
     CDS             complement(545..2389)
                     /locus_tag="PLO35_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOH79789.1"
                     /translation="MKQALVIIILVLLSLPLLAGEWTQYYFRFELLDKAPLKELTKMI
                     SIDNIRGNWIYAYANDAEWEQFSALGYRTQILPAPASEFPAVMASSQAQMRDWDAYPT
                     YDAYVAMMNGFASSYPNLCQIVNVGTTANGRALLMARISDNVSTEEPEPEVLLTSTIH
                     GDELTGYMLLLRLIDTLLSQYGTDSRLTNIVNSMELWIGPNINPDGTYYGGNGTVASA
                     RRYNQNGIDLNRNYPNYDGSLNSGAIQVETQAMMDFANAHNFVFGANYHGGSEVVNYP
                     WDYTYTLHPDDDWFISSSLVYASNVQADGPAGYFTNVSPNGITNGADWYVIAGGRQDW
                     MNYTRNGREVTIECSLTKLPAASTMPSFWNYNYEAMLSYLEQSLYGIHGQVKDAWGNP
                     LAATITVVGHDNSYSIIETDASHGDFYRYLAPGAYNLLIETAGHPSQTVPVTVLANQK
                     TPLAVTFGELPNQQEISLNSGWNLISLNVIPASYQVEEVFEGVSELVQIKDTALSYAP
                     LAADHFNTLSLLSNSRGYWVNVSSPATLTITGAAIDPATVPIALQSGWNLVSYLPDSD
                     LAPAIALASISANLLELRSMEASVPITIMQPGKGYWIKVSAPCTLIYP"
     gene            complement(2386..>4961)
                     /locus_tag="PLO35_08715"
     CDS             complement(2386..>4961)
                     /locus_tag="PLO35_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOH79790.1"
                     /translation="DTDALYTSLPIDGEGSESFTITNTGSQPLNYSISMEELRAGLRN
                     GSLQLASSDRSISGSTLTLDASSYLPGTTVDWTFTVTNASTDTEWLTDVIISFPTGVT
                     VNSATNFVGGDGGDMTPDVTSGSGVSITWHGEGSSGWGVVYGNGDAATATVNVTLGTT
                     IGGNLNLPFTLTGDVYGAEPHTLSDTIVIPEDVPPVEWLSVQPLSGTVPAGGSAVITA
                     SFSAVNMLPGTHEAMLTIHSNDPINSSLEVSATMDVTGTVNHAPSLVLPDAMSFDKNG
                     SLVMDFSAYASDPDSDPLTLQAAGNSNVNVAINGLQVTFTAAQNWTGSELISFSVSDG
                     HLSASDAMTIFVDPVIGPNWPVVTYPNNSATVYGLVTINNVPCQLNDVVGVFVGSECR
                     GTAEVSTNAGNAYVTLLANLAANGETIAFKVWDYETQTTYPVVATYVANFGQVLGSPT
                     PIPINGVDFLGPDSDVSPLSLAFGEVAVGAQSTQQFTISNSGDQPLTGSITTPTAYSV
                     ALAGRANATPASQNVSKTDRITLAYNVAPGLSSTFNLSFAPTAVQAYNGTVSVTSNDP
                     DAPETLISVSGTGFTHPNLIVDLEDIEVSLAWGTSAERNLIISNDGSRVLDFTIVEAT
                     PVEWLSLDPTEGSVSGAAQTITLSFNAGTLAPGDYETTLNIGSNDPDQALVAILVRMT
                     VTNEAPVIALPASCSFPMDQTYVMDFTPYVSDLNGQALSLGCSGNVNVSVIIDGMSVS
                     FSSLNEWYGSEIVTFTVFDGYTYASTSMEVTIELVVPETPVLDPITEVSPDTGVGLSW
                     QPVQYATEYQIWRSLSSPVSGFELIATTTDPSFIDALTHDKAFYMIKAINNPITKGSS
                     K"
BASE COUNT         1042 a         1224 c         1583 g         1112 t
ORIGIN      
        1 cccgccaact cgctgccata tatgctctta tcgaggccgc ttgaactcca acgggtcgag
       61 tggccgcttg aactccaacg tacgtgagcc cgcaaactcg ctgtcatggg tatctttatc
      121 gtggccgctt gaactccaac gtacaatagc ccgcaatctc gttgccatat agacacttat
      181 cgtgactggg cactgcttct gccatcgttt ccagcagatt tttctcccct cccaccgctt
      241 agcattacgg tatcattacg gactcattac ggactaagtc cgtaatgagt ccgtaatagg
      301 atgcggggag gaaggagcaa gaacccggaa agttcgcggt ggcaagcgtt tggcgaagag
      361 gttccaatca ggcaatttga cggagcagtc gaaggcctcc aatccatgaa aaaccctcag
      421 atcggcaggc gcaaaaaagg agcggagtgc gaatccgctc ctcaaaggga taggtgtaac
      481 tgtgcttttc tacgttggac gggggtgccg gggcattaga aaagcccggc gtttgagctc
      541 gaaatcaggg atagatgagg gtgcagggcg cggatacttt gatccagtag ccctttcccg
      601 gctgcatgat agtgatggga actgaagctt ccatagagcg gagctccagc agattggcag
      661 aaattgaggc cagagcgata gctggggcta agtcgctgtc cggcagatag gatacgaggt
      721 tccagccaga ttgaagagcg atgggcacag tggctggatc gatagcggca ccagtgatgg
      781 tcaaagtggc tggggatgag acgtttaccc aatagccccg gctgtttgaa agcagggaga
      841 gggtgttgaa atgatcagca gccaggggag catagctcag agcggtgtct ttgatctgga
      901 ccagttcgct cacgccctca aagacttcct ccacctgata ggaggcgggg atcacattga
      961 ggctgatcag gttccagccg ctgttgaggc tgatctcttg ctggttgggc agctctccga
     1021 aggtgactgc cagaggggtc ttctggttgg ccaggacggt cacgggcaca gtctgggaag
     1081 gatgtccggc cgtttcgatc aggaggttgt aggcccctgg ggccagatag cgataaaagt
     1141 cgccatggct ggcatccgtc tcaatgatcg aatagctgtt gtcgtggccc actaccgtga
     1201 tcgtggcggc cagaggattg ccccaggcgt ctttcacctg gccatggatg ccatagaggg
     1261 actgctccag ataggagagc atggcctcat agttataatt ccagaagctg ggcatggtgc
     1321 tggcggctgg caacttggtg agggagcact cgatggtgac ctcgcgaccg ttgcgggtgt
     1381 agttcatcca gtcctgacgc cctcccgcga tcacatacca gtcggcgccg ttggtgatgc
     1441 cattgggact gacgtttgtg aagtatccgg ccgggccgtc cgcctgaaca ttcgaggcat
     1501 agaccaggct ggatgagatg aaccagtcat cgtcagggtg cagggtgtaa gtgtaatccc
     1561 agggatagtt taccacttcg gagccgccat ggtagttagc accgaagacg aagttgtggg
     1621 cgttggcgaa atccatcatg gcttgagttt ccacttgaat ggcaccgctg ttgaggctgc
     1681 cgtcgtagtt gggatagttg cggttgaggt cgataccgtt ttggttgtag cgccgggctg
     1741 aggccacggt gccgtttccg ccataataag tgccgtccgg attgatgttg gggccgatcc
     1801 agagttccat gctgttcacg atgttggtga gacgggaatc ggtcccatac tggctgagca
     1861 gggtgtctat cagtctcaga agcagcatat agccggtgag ttcgtcgcca tggatggtgc
     1921 tggtgagcag cacttccggc tcgggttctt ctgtggagac gttgtccgaa atcctggcca
     1981 tgaggagcgc gcgtccgttc gcagtggtgc cgacgttcac gatctgacag aggttgggat
     2041 agctggaagc gaagccgttc atcatcgcca cataggcgtc gtaggtggga taggcgtccc
     2101 aatcccgcat ctgagcttga ctggaggcca tcacggcagg gaattccgag gctggagctg
     2161 gcaggatttg cgtcctgtag cccaaagcgc tgaactgctc ccattccgcg tcgttggcat
     2221 aggcatagat ccaattgccg cggatgttgt cgatggagat cattttggtg agctctttga
     2281 ggggggcctt gtccagcagc tcaaaacgga agtaatactg ggtccactct cccgccagta
     2341 aaggcaggga gagcaggacc aggataatga ttacaagtgc ttgcttcatt tggagctgcc
     2401 cttggtgatg ggattgttga tggccttgat catatagaag gccttgtcgt gggtaagggc
     2461 gtctatgaag ctgggatcgg tggtggtggc gatcagttca aagccgctga cagggctgct
     2521 gaggctgcgc cagatctggt attccgtggc atattgtacg ggctgccaac tgaggccaac
     2581 tcccgtgtcc ggagacactt ccgtgatggg atcgaggacg ggggtctcgg ggactaccag
     2641 ttcaatcgtc acttccatac tggtggaggc gtaggtgtag ccgtcaaaca cggtgaaggt
     2701 gactatctcg ctgccatacc attcgtttag agagctgaag ctcacgctca tgccgtcgat
     2761 gatgacgctc acgttcacgt ttccggaaca gcctaaagag agggcctggc cattgaggtc
     2821 tgagacgtaa ggtgtgaagt ccatcacata ggtctggtcc atggggaagc tgcaagacgc
     2881 tggcaaagcg atgacgggag cttcattggt gacggtcatc cgaactagga tggcgacgag
     2941 agcttggtcc ggatcgttgc tgccgatgtt cagagtagtt tcataatccc cgggtgccag
     3001 agtgccggca ttgaaagaca aggtgatcgt ctgggcagcg ccagatacgc ttccttcggt
     3061 ggggtcaagg ctcagccatt ccactggagt ggcttccacg atagtgaaat ccaggacacg
     3121 gctgccgtcg ttggagatga tgaggttcct ctctgcagag gttccccagg cgaggctgac
     3181 ttctatgtct tcaaggtcca caatgaggtt gggatgagtg aatccggtac cggagacgct
     3241 gatgagagtc tcaggagcgt cggggtcgtt gctggtcacg ctgacggtgc cattataggc
     3301 ttgaacggca gtgggagcga agcttaggtt gaaggtggag ctcaggcccg gggccacatt
     3361 gtaggccagg gtgatgcggt ctgttttgct gacattttgg gatgccggtg tggcattggc
     3421 gcgtccagcc agggctacgc tgtaagctgt aggagtggtg atggaacccg tgaggggctg
     3481 gtctccgcta ttggaaatag tgaactgctg ggtggattga gcgcccacgg ccacttctcc
     3541 aaaggcaagg gagagagggc ttacatcgct gtccgggccc aggaagtcca ctccgttgat
     3601 gggaatggga gtggggctgc cgagcacctg accgaagttt gccacatagg tagcgacgac
     3661 gggatatgtg gtctgggtct cataatccca caccttgaag gcgatcgttt ctccatttgc
     3721 ggcaaggttt gccagcagag tcacataggc gtttccggca ttggtgctta cctcagccgt
     3781 gccgcgacac tcggatccca caaacacgcc caccacatcg ttcagctggc aggggacgtt
     3841 gttgatggtg acgaggccat agacggtggc ggagttgttg ggataagtca ctacaggcca
     3901 gttcggtcca atcacgggat caacaaagat cgtcatggca tcggaggcgc tgaggtggcc
     3961 atcgctgacg ctgaagctga tgagctcgct accggtccag ttttgcgcgg cggtgaaggt
     4021 gacctgtaag ccgttgatgg ccacgttcac attgctgttt ccagcggcct gcagggtgag
     4081 gggatcgctg tccggatcgg aggcgtaagc gctgaaatcc atcaccagac tgccgttctt
     4141 atcaaagctc atggcatccg gaagcaccag gctgggcgcg tgattcacgg ttccggtcac
     4201 atccatggtg gcgcttactt cgaggctgga attgatggga tcgttggaat ggatggtgag
     4261 catcgcctca tgggttccag gcaacatgtt gacagccgag aaagatgctg tgatcacagc
     4321 agatccacct gccgggacag tccctgacag gggttggacg ctgagccatt cgacaggcgg
     4381 gacgtcttcc gggatcacga tggtgtctga gagggtgtgg ggctcggcac catagacgtc
     4441 gccggtcaga gtgaaaggca gattcaggtt tccaccgata gtagttccga gcgttacatt
     4501 tactgtggcc gtggcggcat cgccattgcc ataaaccacg ccccagcctg aagatccctc
     4561 gccatgccag gtgatggaga ctccgctgcc ggaagtgaca tcaggagtca tatccccacc
     4621 atctccaccc acaaagttcg tggcgctatt gacggtcact cctgtgggga agctgatgat
     4681 cacatctgtc aaccattcag tatccgtgct ggcattggtg acggtaaagg tccaatccac
     4741 cgtagttcca ggcagataag agctggcatc cagtgtaagc gtgctgccgc taatgctgcg
     4801 gtctgatgag gcaagctgaa ggctgccatt tctgaggccg gcgcgcagct cttccatgct
     4861 gatgctgtag ttcagaggct ggctgccggt gtttgtgatg gtgaagctct cagagccctc
     4921 gccatcgatg ggaagcgatg tatagagcgc atcggtgtcc a
//
LOCUS       DAOSFN010000159         4922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_15288_length_4922_cov_4.681323, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000159 DAOSFN010000000
VERSION     DAOSFN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15288_length_4922_cov_4.681323"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..>4922)
                     /locus_tag="PLO35_08720"
     CDS             complement(11..>4922)
                     /locus_tag="PLO35_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH79791.1"
                     /translation="QSCQFHNDASLNTSNREAIYVTADGSSDADNLNVSLNAFYNGSA
                     HIYTNTSSSLTTFSNWSIYLNYHSGGYYGLYLKQVFNLSINAMISQNINTAVYLDACG
                     GDIDIQRNNIAANITGIYMNYCGNISSASPGIVNNIIRISGAGWYNPNADSDSQGIYL
                     YFSDNIKIYHNSIHNESLTTGSVCLGTFGSDNLIRKNNLASTGLGYTIFVWSLAANTV
                     EYNNLWTSNINLAKLQNDYVTDLLTFNDLAGSANLAYYPFFTDDQLRTVAPKLDDYGP
                     SFGITTDFNNVPRDASNPDIGAHEFSSDPALLPLSGTVYVGVGQTYGTLQEFMTALSF
                     RGVSGLLTAQLTDTLYVEQVEGFGIPGSNPSYLVQVKGHETLGSTLRWDDQTVGDNFV
                     MRLKRMTHIDFHDIRFMTASTQYANLVWMPGYNYKTRFLDCMFTAPVGAQGSGILSDY
                     QDLNLDFSVLSSGFYNNRYGLQHGGDLLTVGSSWFFGQYMGMNISSSDQCEIAGNIFE
                     GHSNSAITCNGGNRWKIYSNKVKAAGAGFLLYNLDLFEDQSNLIYNNTIHVTSGNQYN
                     GMALGGSGIKILNNSILCEATNAAALYMYQPGSQIEVINNIFQATLGLALEYVYYSPS
                     ADRVMDYNSFYSLGNNIVRLGGNYYGSLTALIAGDPTNNIHSWFINPHFTTDLHTQSP
                     WLREIGMPRTEFDNDMDYQLRGSLWDIGADQQTGPSGFTPLAGTYSVGPGGDYPGIPQ
                     FLNDLHLLGSGDDIVVNLLPGVYTGYHTLSDYPRLYPSSRLYFNAMPGASFELIPQNT
                     YGYQNYIFRLQGIDKLTLSGHSFNVAASTRQSVLILLEGKCDDIVINECGFNMGSQSS
                     VAIQASQSVSDGLSILDCDFLGGSKAAELSGVNASGNYYQNVRIQSCSTQGTYTPFTL
                     NAMNNLKLLYNSFSDFSGSPSLSYAYGNTDIVSNKLRAHNFAGSYSSLVMLSLSNLYG
                     TQDQPIEILNNLVYSAGNQVQSCTGINLYGGTHIRLYHNSLILENQYIFQYGSALALS
                     NVSNLRAVNNIFSNSHTGYAIYTQSCSDLEFYHNAYYSGHPKLGKIDSTECETGDLIY
                     TQLQDIDGISADPLPDAQGYSTCQYLADKGMESYVMLDIDQAIFGSNPSIGANVITPA
                     PAFNGTVTVGPSGDFSSLDTALDALMSRGIEGNTQVYLQSGFYQLNQRLGFIPNTLTH
                     TLTITGSPTAYIYGSASTASTNYTLALKNTRNLILDGLNIAPQQEAFSRAIAIERFCE
                     DVSIQNCHITMAANSLSTDASAGIYSDNAMIRDLRVQNCQIENFSRGVLLSASSSFPN
                     ESSLVELLDNEISGSYYGVNLYNLPAPVLNANQISSFRHTGINMSSNRGAAQIKRNSV
                     SGNGYYALYFNNHSAGAQRTVVATNYLSCVSSGWGYAMQLSGSPDVDVYFNTFRIQSP
                     SSDAYGFYHSGSSGLNFACNLAQGSNSYAAVFSTPASVQRKDHNLFYSTSGNAVKFGS
                     TDINNLGAWSAATSDLTCLFTDPLLAAESYSFPMNSPAINAATVIDGLEWDILANWRD
                     NPDIGCWEYAVMGIETPVNLQISIDPVSSEVLLSWDPVAGATSYKVWYAASPDSASWS
                     YISVSVPSARLAASSTQRFYKVTAVN"
BASE COUNT         1067 a         1162 c         1501 g         1192 t
ORIGIN      
        1 aatgatcaga tcagttgact gccgtcacct tatagaatct ctgagtggag gaagcagcca
       61 gacgggcaga gggcacgctc acgctgatgt agctccagct tgcggaatcc ggtgaggcgg
      121 cataccacac cttataggat gtggcgcctg ccacgggatc ccagctcagc aggacttccg
      181 agctgacggg atcgatcgag atctgcagat taaccggcgt ctcgattccc atcacggcat
      241 attcccagca accgatatcg gggttgtctc tccagtttgc cagaatatcc cactccaggc
      301 catcgatcac ggtggcggca ttgatggccg gagaattcat cgggaagctg tagctttcgg
      361 cagccagcaa gggatcggta aagaggcaag tgaggtctga agtggcagcg ctccaggcac
      421 cgaggttgtt gatatccgtg ctgccaaatt tgacggcgtt tccgcttgtg ctgtagaaga
      481 ggttgtggtc cttgcgctgc acagaagccg gtgtggaaaa tactgccgcg tatgagtttg
      541 agccttgggc aaggttgcag gcgaagttga gaccgctgct accgctgtga tagaagccat
      601 aggcatcgct gctgggagac tgtatccgga aagtgttgaa atacacatcc acatccggac
      661 taccgctcaa ttgcatggcg tatccccagc ccgagctcac gcagctcagg tagttggttg
      721 ccaccacagt cctttgagct ccggcgctgt ggttattgaa gtataaggcg taatagccgt
      781 taccgctcac gctgttgcgt ttgatctgag cggctccgcg attggaactc atattgatgc
      841 cagtatgcct gaagctgctg atctggttgg cattcaagac aggggcgggg agattgtaga
      901 ggtttactcc gtaataagat ccgctgattt cgttgtccag tagttccacc aggcttgact
      961 cgttgggaaa ggatgaactg gccgagagca gtaccccccg ggagaagttt tcgatctggc
     1021 aattctggac cctgaggtcc ctgatcatcg cgttatcgga ataaatcccg gcactggcat
     1081 cggtgctgag gctgttggca gccatggtga tatggcagtt ttggatgctg acatcctcgc
     1141 agaaacgctc gatggcgata gcccgtgaga atgcttcctg ctggggagcg atattgagac
     1201 cgtccagaat cagattgcgg gtgtttttga gggccagggt gtagttagtg ctggctgtgg
     1261 aagcactgcc atagatgtag gcagtgggcg atcctgtgat ggtgagcgtg tgggtgaggg
     1321 tgttgggaat aaagcccagg cgctggttga gctgatagaa accactctgt aaatagacct
     1381 gggtgttgcc ctctatccct ctggacatca gagcgtccag agcggtatcc agcgagctga
     1441 aatctccgct ggggcccaca gttacggtcc cgttgaaagc gggtgccgga gtgatcacgt
     1501 tggcaccgat gctggggttg ctgccaaaga tggcctggtc gatatcgagc atcacatagc
     1561 tctccatgcc tttgtctgcc aaatactgac aggtgctgta gccttgggca tcgggaagcg
     1621 gatcagcaga gatgccgtca atgtcctgaa gctgagtgta gatcaggtct ccggtttcac
     1681 attccgtgga gtcgatcttg cccagtttgg gatgaccgct gtagtaggca ttgtgataga
     1741 attccaggtc gctgcagctt tgagtgtaga tggcgtagcc ggtgtgggag ttgctgaaga
     1801 tgttgttgac ggccctgagg ttgctgacgt tgctcagtgc cagcgcgctt ccatattgga
     1861 aaatgtattg gttttccaag atcaggctgt tgtgatagag acggatgtga gtgccaccgt
     1921 aaaggtttat ccccgtgcag ctttgcacct gattgccggc tgaatagacg aggttgttca
     1981 gaatctcgat gggctggtcc tgggtaccat agaggttgct gaggctcagc atcaccaagc
     2041 tggaatagct gcccgcgaag ttgtgggccc gcagcttgtt gctcacgata tcagtgtttc
     2101 cgtaagcata gctgagggag ggtgagccac tgaaatccga gaaactgttg tagagcagct
     2161 tgaggttgtt catggcattc agggtaaagg gagtgtaggt cccctgggtg ctgcaggatt
     2221 ggatgcgcac gttttgataa tagttgccgc tggcattcac accactcagt tcagcggctt
     2281 tggaaccacc caggaagtcg caatccaaaa tgctgagtcc atcgctcaca ctctggctgg
     2341 cctggatggc cacactggac tggctgccca tattgaagcc gcattcgttg attacgatgt
     2401 cgtcgcactt gccttccagc aggatgagca cgctctggcg ggttgaggca gccacgttga
     2461 agctgtggcc gctgagggtg agcttgtcga tgccctgcag cctgaagata tagttttgat
     2521 agccgtaggt gttttgggga atgagctcga aactggcccc cggcatggcg ttgaagtata
     2581 atctggagga gggatagagc ctggggtagt cactcagggt gtgataaccc gtatagactc
     2641 ccggcaggag gttcacgacg atgtcgtcac cgctgcccaa taggtgcaga tcattcagaa
     2701 actgcgggat gcccggatag tctccacccg gccccacgct gtaagttcct gcgaggggag
     2761 tgaaacccga aggcccggtc tgctggtcgg ccccgatgtc ccaaaggctg ccacggagct
     2821 gataatccat atcattatca aactccgtcc tgggcatgcc aatctcccta agccagggac
     2881 tttgggtgtg cagatcggtg gtgaaatggg gattgataaa ccatgaatgg atgttgttgg
     2941 tgggatctcc agcgatcaga gcggtcagac tgccataata gttgccaccc agccggacga
     3001 tattgtttcc caggctgtag aagctgttgt agtccatcac ccggtccgcg gaagggctgt
     3061 aatacacata ttccagcgcc aatcccaggg tggcctggaa gatgttgttg atcacctcga
     3121 tctgagaacc gggctggtac atatagaggg ccgcggcgtt ggtagcctcg cataagatac
     3181 tgttgttgag tatcttgata ccgcttccgc ccagggccat accgttgtac tgattgccgg
     3241 atgtcacatg gatagtgttg ttgtagatga ggttggattg gtcctcaaag aggtcgaggt
     3301 tatagagcag aaaacccgct ccggcagcct ttaccttgtt gctgtaaatt ttccaccggt
     3361 tgccgccgtt gcaggtgatg gcggaattgc tgtggccctc aaagatgttg ccggcgatct
     3421 cgcattggtc tgaggaactg atattcatgc ccatatactg gccaaaaaac cagctgctgc
     3481 ccaccgtgag caaatctccc ccatgctgaa ggccgtagcg gttgttataa aaaccgctgc
     3541 tcagcacgct gaaatccaga ttgaggtcct gataatcaga caggatgccg ctgccctgag
     3601 cgccaacagg tgctgtgaac atgcagtcga gaaagcgggt tttgtagttg tatcccggca
     3661 tccagaccag attggcatat tgggtggagg ccgtcataaa gcgaatatca tgaaagtcta
     3721 tgtgggtcat gcgtttgagc cgcatcacaa agttatcacc gacggtctga tcatcccatc
     3781 tgagagtgga acccagggtc tcatgtcctt tcacctgaac cagatagctg ggattggagc
     3841 caggaatgcc aaagccttcc acctgttcca catagagggt gtcggtaagc tgcgcggtga
     3901 ggagtccgct cacgcctctg aagctcaggg cggtcataaa ttcctgaaga gtgccatagg
     3961 tctgccccac tcccacatac acggtcccgg agagaggtaa caaagccgga tcggaactga
     4021 actcgtgagc tccgatatcg ggatttgagg cgtctctggg gacgttgttg aaatcggtcg
     4081 tgatcccaaa ggagggaccg tagtcatcca gcttgggggc cacggtgcgc aactgatcat
     4141 cggtgaagaa gggatagtat gccaggttgg cagagccggc caggtcgttg aaagtcagca
     4201 ggtccgttac gtagtcgttt tgcagtttgg ccaggttgat gttgctggtc cagaggttgt
     4261 tatattccac tgtgttggcg gccagactcc acacgaatat ggtgtaaccc aatccggtgc
     4321 tggccaggtt gtttttgcgg atcaggttgt cggagccaaa ggtgcccagg caaacagaac
     4381 cggtggtgag agattcgttg tggatgctgt tgtggtagat cttgatgttg tcagaaaagt
     4441 agaggtaaat gccctggctg tctgaatccg cgttgggatt gtaccagcct gcaccgctga
     4501 ttctgatgat gttgttgacg atgccggggc tggcgctgga gatattgccg cagtagttca
     4561 tatagatgcc ggtgatattg gcggcgatgt tgttgcgctg gatgtctatg tctccgccac
     4621 aagcgtccag atacaccgcg gtgttgatgt tttgtgatat catggcgttg atgctgaggt
     4681 tgaatacctg tttcagataa agcccgtaat agccgccgct gtgatagttg aggtagatgc
     4741 tccagttcga gaaggtcgtg aggctgctgg aggtattggt atagatatgg gcagaaccgt
     4801 tgtaaaaggc attgagagac acattcaggt tatccgcgtc gctggagcca tccgccgtca
     4861 catagatggc ctcgcggttg ctggtattga ggctggcgtc gttgtgaaac tggcagctct
     4921 gg
//
LOCUS       DAOSFN010000160         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_15299_length_4920_cov_8.853649, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000160 DAOSFN010000000
VERSION     DAOSFN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15299_length_4920_cov_8.853649"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..>1720)
                     /locus_tag="PLO35_08725"
     CDS             complement(97..>1720)
                     /locus_tag="PLO35_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOH79792.1"
                     /translation="LSPARSALTLLTLLLASTLSANIVINELMYDPNGTDTGYEWLEL
                     YNNGLSDIDLEGARLLKAGSAYEEVFVFPRFILRAGRYLLLGETNVPNAQFTASLAFQ
                     NGGSETDAVRFESADGLYRDTVLYDEPNYYELPDDSGVSGSSFAPDAPQGYALARIMD
                     GWDSNLCGVDFIAEAYPSPGSANPVRIDYGLYWPSLRQDEDSWICGIWVKNLSPISAY
                     LEIPLEFRLDGVSAGTICACNISAGDSLFYENWFAIADEDNHLIEISLDLASDPDQTN
                     NQISLQLYGPELQGPRLNEVMYRPATGFQEWIEIWQGSPYRGSYRIEDRAGNGFDFEL
                     PDQAGYFVVCASPAQLLSRYPDCPADIVIDVSGWATLNNDGDEVWLLDADGETIDSLS
                     YEADATPPDRSLERYESQGMSLWRACLASTGATPGLVNSSPVEVPPGQSKPVAVYGSP
                     CQARAGEQISVSYQLQDSQNRVDCAVWSRAGQLVRVLADNTLLPNTGVLSWDGRDANG
                     KYVPRGLYYISWTSRGLSSGKEIYKRFSVAMR"
     assembly_gap    1721..1820
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1905..4406)
                     /locus_tag="PLO35_08730"
     CDS             complement(1905..4406)
                     /locus_tag="PLO35_08730"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOH79793.1"
                     /translation="MKEGFIPKNDFHSIFFTDATESIGRDIKSQFLSHLEYSLVKDTT
                     NVQAWDVYYALSLTLRDRLIERWLRTQYEYRKQDVKKVYYLSMEFLIGRLLGNTLINL
                     DAYDGVYDMLRQMGHSLEDIVEEEPDMGLGNGGLGRLAACFMDSLATQAYPAYGYGIR
                     YEFGIFKQNIVQGYQVEEPDHWLKKGCPWEIQRPEISYRVRFGGRVLSEEQSDGRVLH
                     RWVDTEDVMAVAWDIPIPGYKVDNVNNLRLWQATATDEFDFDYFNSGDYVKAVEKKNI
                     SENISKVLYPNDNMHLGRILRLKQEYFFVSATLQDIFAAWKRDHDSFRDLPSKIAIQL
                     NDTHPAIAIPEMLRILIDEERLSWEDAWQITRGVFSYTNHTILPEALERWSVKLFEEL
                     LPRHLMLIYQINNQVLGEITRLYPGNIVRMRNMSIIEEGPEKSLRMAQLCLHGSHTVN
                     GVAQLHSNIIKERIFPDFYEMRPEMFQNKTNGITPRLWLRASNPQLASLITEHIGDSW
                     VRDLDHIRGIEQYADDKDFLDGFLEVKGINKKNLARHIYRATGIRVQTNALFDVQIKR
                     LHEYKRQLLNVLGTLARYWRIKHEPGGTYVPRVVIFAGKAAPGYFMAKRLIKLINSIA
                     DVINKDPDVKDRLKVVFLPNYTVSLAEKIIPAADLSEQISTAGYEASGTGNMKFALNG
                     ALTIGTLDGATVEMAEEIGEENMLLFGLKAGEVSALKQSGYDPWKYYNEDPVLKEVID
                     SIRGDLFCPNEPGIFVSIAQSLLENGDPYLMLADFQSYLAASQKADELYAQPREWARK
                     AIINIARSGKFSADRAIREYAEDIWKIRPLLLDLH"
BASE COUNT          963 a         1308 c         1432 g         1117 t
ORIGIN      
        1 tgccactcca ctagctggcg cgctcgcgta acgggtaacg catcacccat aacgcatcac
       61 ccataacgct taacgcatac cgcgtaacga cccctcctac ctcatcgcca cgctaaagcg
      121 cttgtagatt tccttgccgc tgctcaggcc cctggacgtc caggagatgt agtagagccc
      181 tctgggcacg tacttgccgt tggcatcgcg gccatcccaa ctcaggacac cggtgttggg
      241 taaaagggtg ttatccgcca gcactctgac cagctggccc gctctggacc acaccgcgca
      301 gtccacgcga ttctggctat cctgaagctg gtaggagacg ctgatctgct ctccggccct
      361 ggcttggcag ggactgccat agacggccac gggtttggac tgcccgggtg ggacctctac
      421 gggagagctg ttgacaagtc ctggagtggc tccagtgctg gccagacaag ctctccagag
      481 gctcatgccc tggctttcgt aacgctccag tgaacggtcc ggtggagtgg catcggcttc
      541 atagctcagg gaatcgatcg tctcgccgtc cgcgtccagc agccagactt catcgccgtc
      601 gttattgagc gtagcccagc cacttacgtc gatcacaata tccgccgggc agtctggata
      661 gcggcttaga agctgagcgg gagaagcgca gaccacgaag tatccagcct ggtccgggag
      721 ctcaaaatca aagccgttcc cagccctgtc ctcgatcctg tagctgcctc ggtaagggct
      781 tccctgccag atctcgatcc attcctgaaa tcccgtggcg ggacgataca tgacttcgtt
      841 gagccgcggg ccctgcagtt ccggcccata aagctgcaga ctgatctggt tgttggtctg
      901 atcggggtcc gaggccaggt ccagactgat ctcgatcagg tggttgtcct catcggcgat
      961 ggcaaaccag ttttcataga agagggagtc tccagcgctg atattgcagg cgcagattgt
     1021 accagcactc acgccatcca gcctgaattc cagagggatt tccaggtagg cggagatggg
     1081 cgagagattt ttgacccaga tgccgcagat ccagctatcc tcatcctggc gcaggcttgg
     1141 ccagtagagg ccatagtcta tgcggacagg attggccgaa ccgggactgg gataggcctc
     1201 ggcgataaaa tccactccgc agaggttgga atcccagccg tccatgatgc gggccagagc
     1261 atatccctgg ggcgcatcgg gagcaaaact gctgcccgaa actccgctat cgtccggcaa
     1321 ttcatagtag ttgggctcgt catagaggac cgtgtcgcga tagagtccgt cagcgctctc
     1381 aaagcggacg gcatccgtct cgctgccacc gttttggaag gccaggctgg cagtgaactg
     1441 ggcgttgggc acattggtct cacccagcag cagatagcgt cccgctctga ggataaagcg
     1501 cgggaacaca aagacttcct cataggcgga gccggctttg aggaggcgcg cgccctccag
     1561 atcgatgtcg gagaggccgt tgttgtagag ctccagccat tcatagccgg tatccgtgcc
     1621 gttggggtca tacatgagct cgttgatcac aatattggcg gagagggtgc tggcgaggag
     1681 gagggtgagg agagtgagag cgcttcgcgc tggtgagagg nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn tctcatgttc tcatatctca ttgtttgcca atctcattct
     1861 ctcatattct cattgttcaa aactcactca tggatcacac agtatcagtg caggtccagc
     1921 agcagcggcc tgatcttcca gatatcttcc gcgtattcgc ggatagctct gtcggcagag
     1981 aacttaccgg atcgggcgat gttgatgatc gccttgcggg cccactctct gggttgcgca
     2041 tacagctcat cggccttttg cgaggcggcg aggtagctct gaaaatcggc cagcatcaga
     2101 tagggatcgc cgttttccaa cagcgactgc gcgatggaga caaagatgcc gggctcgttg
     2161 gggcagaaga ggtcaccccg gatgctgtcg atcacctctt tgagcaccgg gtcctcattg
     2221 tagtatttcc agggatcgta gccggattgt ttgagggcgc tcacttcccc ggctttgagg
     2281 ccaaagagga gcatgttttc ctcgccgatc tcctcggcca tctcaaccgt agcgccgtcg
     2341 agggtaccga tcgtgagggc cccgttgagg gcaaacttca tattgccggt accgctggct
     2401 tcatagccgg ccgtggaaat ctgttccgat agatcggccg cggggatgat cttttcggcc
     2461 agggaaactg tgtaattggg cagaaacacc accttgaggc gatctttgac gtcggggtcc
     2521 ttgttgatga cgtcggcgat ggagttgatc agcttgatga ggcgtttggc catgaaatat
     2581 ccgggggcgg ccttcccggc aaagatcacc actctgggca cgtaagttcc accgggctca
     2641 tgcttgatcc tccaatagcg ggccagggtg cccaacacgt tgaggagctg gcgcttgtat
     2701 tcatggaggc gcttgatctg gacgtcgaag agggcgttgg tctgcactct gattccggtg
     2761 gcgcgataga tgtggcgggc cagatttttc ttgttgatgc ccttcacttc caggaatcca
     2821 tccagaaaat ccttgtcgtc ggcatactgt tcgatgccgc ggatatggtc cagatcacgc
     2881 acccagctgt ctccaatgtg ttcggtgatg aggctggcca gttgggggtt ggaagccctc
     2941 agccacagcc ggggggtgat cccgttggtc ttgttttgaa acatttcggg gcgcatctca
     3001 tagaaatccg gaaagatacg ctctttgatg atgttggagt gcagttgcgc cacgccattg
     3061 acggtgtggg agccgtgcag acagagctgg gccatgcgca ggctcttttc aggcccttcc
     3121 tcgatgatgg acatgttgcg catgcggacg atgttgccag gatagagacg ggtgatctct
     3181 cccagcacct ggttgttgat ctggtagatc agcatcagat ggcgcggcag cagctcttca
     3241 aagagcttga cgctccaccg ctccagggct tccggcagga tggtgtggtt ggtataggaa
     3301 aacacgcccc gggtgatctg ccaggcgtct tcccaactga gccgttcttc gtcgatgaga
     3361 atgcgcagca tttccgggat ggcgatggcg ggatgggtgt cgttgagctg gatggcgatc
     3421 ttgctgggaa ggtctctaaa gctgtcgtga tcacgcttcc aggcggcaaa gatatcctgg
     3481 agggtggcgc tcacaaagaa gtattcctgt ttcagcctca ggatgcggcc caggtgcatg
     3541 ttgtcgttgg ggtacagcac tttggagatg ttttcgctga tgtttttctt ttccacggcc
     3601 ttcacataat cgccgctgtt gaagtaatcg aagtcaaatt catcggtggc ggtggcttgc
     3661 cagaggcgca agttgttgac gttatccact ttatagcctg gaatcgggat gtcccaggcc
     3721 acggccatca cgtcctcggt atccacccag cgatgcaaaa cccggccgtc cgactgctct
     3781 tcgctcagca cccggccgcc aaaacgcacc cgatagctga tctccggacg ctggatttcc
     3841 cagggacaac ccttcttgag ccagtgatca ggctcttcca cctggtagcc ctgcacgatg
     3901 ttttgcttga agatgccaaa ctcgtagcgg atgccataac cgtaagcggg ataagcctga
     3961 gtggccagtg aatccataaa acaggcggcc aggcggccca gacctccgtt gccgagtccc
     4021 atatccggtt cctcttccac gatgtcttcc aggctgtggc ccatctggcg taacatgtcg
     4081 taaactccgt cgtaggcgtc cagattgatc agcgtgttgc ccaaaaggcg gccgatcagg
     4141 aactccatcg agaggtagta gacctttttc acatcctgct tgcgatattc gtattgggtg
     4201 cgcagccagc gctcgatcaa cctgtccctc aaagtgagcg agagggcgta atagacgtcc
     4261 caggcctgga cgttggtggt atccttcacc agcgaatact cgaggtgact gagaaactgc
     4321 gactttatat ctctaccaat gctttccgtg gcatccgtga aaaagatgga gtggaagtca
     4381 ttctttggta tgaatccttc tttcatgggg tttcctttgt ctgtgttcta agacgactta
     4441 ccagattgag ggatggtgac ttggtgtcaa gtggtttttg ctttcaggtg agagaactgc
     4501 gtgctggtga gaggcttcgc ctggtgagaa gtcggcagct gctgactggt ggagtagcaa
     4561 acgtcctcgt ttgctcaggc gcgaacagcg cctgtggcat ggcctccaga gcaggctgcg
     4621 ttggtgtatg ttagggtgga gggctatcct ccctcggtaa cgagacgttt ccggtcccac
     4681 aaacatcctc cctcggtaac gagacgtttc cggctccctg ggtatggttt ctgctttgct
     4741 ctacgggtgc tctacgggtc cgacccgtag agcacccgta gagcacccgt aataggatgc
     4801 ggggggatca gttcattcac ttctccggtg ttgagatgtt tgagagggag gttcccgcag
     4861 ataacgcaga tttaagcgcg gatttcgcag ctggtaaaga gggcggtgct acagcgagcg
//
LOCUS       DAOSFN010000161         4913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_15326_length_4913_cov_9.415191, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000161 DAOSFN010000000
VERSION     DAOSFN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15326_length_4913_cov_9.415191"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..623)
                     /locus_tag="PLO35_08735"
     CDS             complement(6..623)
                     /locus_tag="PLO35_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOH79794.1"
                     /translation="MTLRIHRKTEKGRTALVYVDDHCVGTLPVKVLPPYVIEGRELKA
                     QPWQIDELLNLIKNQARDTLLDYLAKAEHSEWQSRELLKRHRFGDILIDELIAYCKEH
                     SFIDDARFAEIYIRSWLNRGTGKRLLISKLYEQRIAPAIWQPILAELYDRDDAQDSLA
                     SQMRAYISRQKDISSQKLKDKVFSHFIRKGFDLDQIAQAWTRRDE"
     gene            complement(595..1638)
                     /gene="recA"
                     /locus_tag="PLO35_08740"
     CDS             complement(595..1638)
                     /gene="recA"
                     /locus_tag="PLO35_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOH79795.1"
                     /translation="MDKNKESALKTAVSQLEKKFGLGTIMRLGDKPEVVVQVIPTGAF
                     NLDLALGIGGIPKGRITEIYGAEASGKTTIALHVAAEAQKQGGVVAFIDAEHALDIHY
                     AKRLGVQTDDLLLSQPDGGEQALEVVETLVRSSAVDLVIVDSVAALVPRQEIEGEMGD
                     SHVGLQARLMSQALRKLTAIVSKSNTAVIFINQTRMKIGVPAYVNPETTTGGVALKFY
                     SSVRLEVRFAGAIKIAAADSDVVGAKTRVKVVKNKFAPPFKTVVFPIIFGEGISHLDI
                     IMEMAIEMEIIKKSGSWFSYEDTKIGQGAEKAKLYLKENPPILAEVEARVKDKASPEL
                     IAKLDDAEDTPQD"
     gene            complement(1653..2204)
                     /gene="thpR"
                     /locus_tag="PLO35_08745"
     CDS             complement(1653..2204)
                     /gene="thpR"
                     /locus_tag="PLO35_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOH79796.1"
                     /translation="MTYRCFFALEVPEPLRANLVHTLRDISKIPGVNWVPPQNLHLTL
                     LFLGDVKQELVPALTQIQLDSSDIWKSISMSARGIELFPAKAPRLVWVNLQAEDRSIF
                     DLHKELGRQIRALGIELDGKALKLHITLGRIKAQLPPQVERSLIESPLDTQYLIYDQL
                     TLYRSVLRPEGPVYSIIEQSKLR"
     gene            complement(2205..2687)
                     /locus_tag="PLO35_08750"
     CDS             complement(2205..2687)
                     /locus_tag="PLO35_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOH79797.1"
                     /translation="MELKLEQLKFPEDTNIILGQSHFIKTVEDLYEAMVNSVPGVKFG
                     LAFCEASGPCLIRKDGTDNELIELAVENLLTLGAGHSFLIIMRGAYPINVLPAIKDCR
                     EVVNIFCATANPVQVILAQSEQGSGILGVIDGSSPLGSELDTDISHRKKFLLDIGYKR
                     "
     gene            complement(3149..4810)
                     /locus_tag="PLO35_08755"
     CDS             complement(3149..4810)
                     /locus_tag="PLO35_08755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH79798.1"
                     /translation="MLKFTDLALAQPLLRAAADLGFEKPTPIQADVIPWLLDNDGDLI
                     ALAQTGTGKTAAFGFPVLSATNIEATQPQTLILCPTRELCLQIYSDFQDYAKYMPRVK
                     SVAVYGGAALLPQKKALQAGTQIVIGTPGRVQDFIRQGVLKLERVEFLVLDEADEMLN
                     MGFKEDLFSIMEHTPKDKQTMLFSATMPSEVSKLASTFMKNPHQVSVAHSERGAQNIL
                     HFYYKVQARDKYLALKRIADMNPEIYGIVFCRTRNETQDIADKLQQDGYNADALHGDL
                     SQAQRELVMNRFRTRYVRLLVATDVAARGLDVDDLTHIINYHLPTDPDIYIHRSGRTG
                     RAGKHGIALSIIHSKEFNELKAIEKRLGRDIEFSKVPDGQAICEKQLFNFIDTVEKVE
                     VEAEQIEPFLRNVYKKLGWMTREELIQRFVSVEFNRFLSYYKDAGDLNYSEERVSKAR
                     KGNVSFLTFRLGIGYVNDLNKRDLMRYINGLRVTRGIEIGQINIFETHCFVELDAEYE
                     SQLLKAFARNNYRGVPVEAQVSEKRRPERQKPDRTWKKKGPRKDY"
BASE COUNT         1012 a         1268 c         1407 g         1226 t
ORIGIN      
        1 ccctcctact catcccgcct cgtccacgcc tgcgcgatct ggtccagatc aaagcccttg
       61 cggataaagt ggctaaagac cttgtccttg agcttttggc tgctaatgtc tttctggcgg
      121 gaaatatagg ctcgcatttg ggacgcgagg ctatcctgcg cgtcatcgcg gtcatacagt
      181 tctgccagaa tcggttgcca gatagcggga gcgatgcgct gctcgtagag tttgctgatc
      241 agcaggcgtt tcccggtccc gcggttgagc cagctgcgga tgtagatttc ggcaaagcgg
      301 gcgtcgtcta taaagctgtg ttccttgcag taggcgatca gttcatcgat cagaatgtcg
      361 ccaaagcgat ggcgtttcag cagttcgcgg ctctgccatt cgctgtgttc ggctttggcc
      421 agatagtcca ggagggtatc tctggcctgg tttttgatca ggttcagcag ctcgtcgatc
      481 tgccagggct gggctttgag ctctctgccc tcgatcacat aaggcgggag gaccttgact
      541 ggcaaggtcc ccacgcaatg atcgtccaca tagacaaggg cggtgcgccc tttttcagtc
      601 ttgcggtgta tcctcagcgt catcgagctt ggcgatcagt tccgggctgg ctttatcttt
      661 cacgcgggct tccacttccg ccaggatggg aggattttct ttcagataga gcttggcctt
      721 ttcggcgccc tggccgattt tggtgtcctc ataggagaac caggagccgc tcttcttgat
      781 aatctccatc tcaatggcca tctccatgat gatgtccaga tggctgattc cctcgccgaa
      841 aatgatcggg aataccactg tcttgaaagg cggagcaaac ttgtttttga ccactttgac
      901 tctggttttg gcacccacga cgtctgaatc cgcggcggcg atcttgatcg cgccggcaaa
      961 gcgaacttca aggcgcacag aggaatagaa cttgagggcc acgcccccgg tggtggtttc
     1021 gggattcacg taggccggca cgccgatctt catgcgggtc tggttgataa agatcacggc
     1081 ggtgttggat ttggatacga tggcggtgag tttgcgcagg gcctggctca tcagacgggc
     1141 ctgcagaccc acgtggctgt ctcccatttc tccttcaatc tcctgacggg gcaccagcgc
     1201 ggccacggaa tccacgatca ccagatcgac cgcggaactg cgcacgaggg tttccaccac
     1261 ttccaaagcc tgctctccac cgtcgggctg gctgagcagc aggtcatcgg tctggacgcc
     1321 cagacgcttg gcgtaatgga tgtccagagc gtgttcggcg tcgataaagg ccaccacacc
     1381 accctgtttt tgagcttcgg cggccacgtg caaagcgatc gtagtcttgc cgctggcttc
     1441 cgcaccgtaa atctctgtga tgcggccttt tgggatgccg ccaatgccga gggccagatc
     1501 gaggttgaag gccccggtgg ggatcacctg caccacgact tcaggtttgt cgcccagacg
     1561 catgatggtg ccgaggccaa acttcttttc cagttggctc acggcggtct tgagggcgct
     1621 ttctttgttc ttgtccagca ttttacctcc ggttatcgta atttgctttg ttcgattata
     1681 ctgtagacgg ggccctcggg acgcaggacg ctgcggtaga gggtaagttg atcataaata
     1741 aggtattggg tgtccaatgg gctctctatg agagagcgtt ccacctgagg cggcagctga
     1801 gctttgatcc tgcccagggt gatgtggagc ttcagggctt tgccgtccag ctcgataccc
     1861 agagcgcgga tctgtctgcc gagctctttg tggagatcga agatggacct gtcttcagcc
     1921 tgaagattca cccacaccag gcgtggagcc ttggcgggaa acagctctat acctctggcg
     1981 ctcatgctga tagacttcca gatgtcggag ctgtccagct ggatttgagt gagggcgggc
     2041 accagttcct gcttcacgtc tcctaaaaaa agcagcgtga gatgcaggtt ttgaggcggg
     2101 acccagttca cacccgggat cttggagata tccctgaggg tgtggaccag attggcacgc
     2161 agcggttcag gaacttccag cgcgaaaaag cagcgatagg tcattcagcg cttgtagcct
     2221 atatccagca ggaatttctt gcggtggctg atatcggtgt cgagttcact gcccaggggc
     2281 gaggaaccgt cgatcactcc caggatgccg cttccctgct cgctctgggc caaaatcacc
     2341 tgcaccggat tggcggtggc gcaaaagatg tttaccactt cgcggcagtc tttgatggca
     2401 ggcaacacgt tgatcgggta ggcgccccgc atgatgatca aaaagctatg cccggcaccc
     2461 agggtgagca aattctccac cgccagttcg attaactcgt tatccgtgcc gtccttacgg
     2521 atcagacaag ggcccgaggc ttcgcaaaac gccaggccga atttgacccc gggcacgctg
     2581 ttgaccatgg cctcatagag gtcctccacg gtcttgatga aatggctctg gcccaggatg
     2641 atattggtgt cttcgggaaa tttgagctgc tcaagcttga gttccatgct cctatcctca
     2701 ctagtttatg gcaaaattgt ttagccaatt aaaaaaggga gccatccgga gtcaagcaaa
     2761 actttgtgga gcttaggagt tttagggttt tagagtttta gggagctccc ggtccccaaa
     2821 tctctgctat cttctggggt tggggagctc ccggtcccca aatctctgct agctctgagg
     2881 ttctaaaaag aatagaacgc agaataaggg aaaaaaggga tgaaaagagg gatttaatag
     2941 aactcagatg actggatgac tggatttaca ggattaaggg gctgaagtaa atccagccct
     3001 tcaatcaatc tcatttatca catattctca ttgcaagaca atctcattta tctcaatgtc
     3061 tcacattaga aagaactctg cttttctctg cacttccact gcttttctct gtgtttcgtt
     3121 ggatcagaga gtaacagaat ccccgttttt agtagtcttt acgggggcct ttcttcttcc
     3181 aggtgcgatc cggtttctgg cgttcggggc ggcgtttttc gctcacctga gcttccaccg
     3241 gtacgccacg gtagttgttg cgggcaaagg ctttgagcag ctggctttca tactccgcgt
     3301 ccaattccac aaaacagtgg gtctcgaaga tgttgatctg gccgatctcg atgccgcggg
     3361 tcactctgag gccgttgatg tagcgcatca ggtcacgctt gttgaggtcg ttcacatagc
     3421 caatcccgag gcggaaggtg aggaagctga cgtttccctt gcgggctttg ctgacgcgtt
     3481 cttcgctgta gttgaggtca ccggcatcct tgtaatagct cagaaaacgg ttaaactcca
     3541 ccgagacaaa gcgctggatc agttcttcgc gggtcatcca gcccagtttt ttatacacat
     3601 tgcgcaggaa aggctcgatc tgttcggcct cgacttccac tttttccacc gtgtcgatga
     3661 agttgaagag ctgcttttca cagatggcct ggccatccgg cactttgctg aattcgatgt
     3721 cacggcccag gcgtttttcg atggccttga gctcgttaaa ttccttgctg tggatgatgc
     3781 tgagggcgat gccgtgtttg ccggcccggc cagttcgacc gctgcggtgg atgtaaatat
     3841 ccggatcggt gggcaggtgg taattgatga tatgagtgag gtcgtcgacg tccaaacctc
     3901 tggcagcgac gtcagtagcc accagcaacc tcacatagcg ggtgcgaaaa cggttcatca
     3961 caagctctct ctgggcctgc gagaggtctc cgtgcagggc gtccgcgttg tagccatcct
     4021 gctgcagttt atccgcgata tcctgagtct cgttgcgggt gcggcaaaac acgatcccgt
     4081 aaatttccgg gttcatatcg gcgatgcgct tgagggccag atacttatca cgggcctgga
     4141 ccttgtaata aaagtgcagg atattctgag cgccgcgctc gctgtgggcc acgctcacct
     4201 gatggggatt cttcatgaaa gtggaggcca gcttggagac ttccgagggc atggtggcgg
     4261 aaaagagcat ggtctgcttg tctttgggag tgtgctccat gatggaaaag aggtcttcct
     4321 taaaacccat gttgagcatc tcgtccgctt cgtccaacac cagaaattcc acccgctcca
     4381 gtttgagcac gccctggcgg ataaaatcct gcacgcgtcc gggagtcccg atcacgatct
     4441 gggtgccggc ctgcagggct tttttctggg gcagcagagc ggccccgcca tagacggcca
     4501 cgcttttcac gcggggcata tacttggcgt aatcttgaaa gtcggagtag atctgcagac
     4561 agagttcccg ggtgggacac aggataagtg tctgaggttg agtagcttct atattggtag
     4621 cgctgagcac gggaaagcca aaggcggcag ttttaccagt gccggtttgg gccagggcga
     4681 tcaggtctcc gtcgttatcc aataaccatg gaatcacgtc cgcctgaata ggagtaggct
     4741 tttcaaaacc caggtctgcg gcagcgcgga ggaggggttg agctaatgcg aggtcagtaa
     4801 atttgagcaa gtagggggtt ccttataggt agatttgatg tcatgaattt ctaagtgctt
     4861 tatttgtaaa gggaaaaagt gagaactacg ttgtgagatg ctgcgcaggt gag
//
LOCUS       DAOSFN010000162         4905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_15362_length_4905_cov_9.999794, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000162 DAOSFN010000000
VERSION     DAOSFN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15362_length_4905_cov_9.999794"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLO35_08760"
     CDS             complement(<1..932)
                     /locus_tag="PLO35_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOH79799.1"
                     /translation="MFPISFGSDNHSGVHPAVMDSILKANQGYCPAYGDDPITQEALA
                     RVRELFGGACEAWFVMSGTGANVIALQSLISSWHAVACPSSAHINVDECGAVQKFTQG
                     RLQAIDTPDGKLTPELIAPRLYGNRDQHHSQFRVISISQSTEYGSLYTPNEISALADF
                     AHQHDMYLHLDGARLANAAAALNCSLAAITRDAGADIVSFGGTKNGLLFGEAIISFRP
                     EINNAMRFYRKQASQLFSKMRFIGAQFIAYLENELWRANALHANRMCELLGERLAELD
                     QIEITMPPVVNALFCILPQNITQPLMDKYHFYMW"
     gene            complement(993..2195)
                     /locus_tag="PLO35_08765"
     CDS             complement(993..2195)
                     /locus_tag="PLO35_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOH79800.1"
                     /translation="MDMDRDREDYSLDIKTLTREALQAMVEGEWSVHSRDPVYLIKKS
                     WERFSDDGQPLYPNALPEVLLSWAGMDLSTPWKPEDFLVLDLETTGLGRGNTLAFLIG
                     LGYYEGDTYTVEQIFLPDPDAELNSFDRLMELLETHSVLVTFNGKTFDIPVLESRLLY
                     QRLWLNLREKEHLDLLHIARRLWKNQLPSCALESLEFYIMGQIRDKELDISGGLIPQT
                     YYQYLISGDPEPIRRILLHNQLDVLNTAALFGIIGNAIAEPVKTGQDPRVDYLAVARL
                     YQSQGQHETARRILVDLMAQGFCSGPSAYELGMVYKREGDLEGASECFEIASDLEHPA
                     SLLELCMILEKRGELETAVHRTQTLINWHQSRPVPDLKRLSEAARRLERLQRKLEARK
                     AKPRTPNP"
     gene            2439..4145
                     /locus_tag="PLO35_08770"
     CDS             2439..4145
                     /locus_tag="PLO35_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79801.1"
                     /translation="MQEAKDGRIFSSETEHREFVPWWRSGTGIVFLIILLFFVYAAAL
                     IIPRLSSTRTVLVNGLEVLRDIKPTRILISPKEARQMARLHLEKLIGAAPGAELREYA
                     RPWSSDPVSRYYALFENSIDAFYNLLDSYIIREGWRVDFLDTRDNSFPWSVFLDSSGK
                     LLCCQNNPTHPDKPGAITEAEARLTAQSYLTPTVYGSIPSFHESSAVPLPDQHQWLFS
                     FSDSLDHKLVSGRVQLQLVIGAGGVTSFQPGISMPPDHAASIAAAEQLTRRMKIGALV
                     VILLFMIRLFVFTLGRFRRGEWDRGTFWRSLLLLLPMILYNMYDRNHQLRLWVSEPGV
                     PVISGAVFYGAGALFALFISMFLAWSLKWMREREIDPHYMPLVAGRVPLINLSSFLSL
                     LGVILMVVNMGLAHMFSGPSEALPIYSNRISLSFIMFAMYLLILGFMIFIVWRWEQIR
                     RRTAPRIIAGLVAFLVITSIITLSTLPLFKGPFQARLLIMFFCFIPFTGITALLLLYQ
                     MRIRMWYLCSLTWLAAPVLCEFLSPSHKYGGENTFIIVALVALYGWILIVLPERKRKQ
                     TP"
     gene            4217..4579
                     /locus_tag="PLO35_08775"
     CDS             4217..4579
                     /locus_tag="PLO35_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79802.1"
                     /translation="MKKILIVIGILLVAFFIYREFIGIKQLTGENDEYNLKLAIEDHI
                     DPYWLEAGAGVEKIYVDKLEDVKWETIDKDQGLYDCSATIKGTYMVRGSKQAFSVRRK
                     FNVDRSNKIKGYRVKLIE"
     gene            4640..4897
                     /locus_tag="PLO35_08780"
     CDS             4640..4897
                     /locus_tag="PLO35_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79803.1"
                     /translation="MRTGKIIAIECGCGYLLFRYYKAGKGRLIKCLISRLSQDNVGLQ
                     GAATLSRPLCPSCGKELGIIMMIHGEPALKLNQGTIRSVRV"
BASE COUNT         1056 a         1305 c         1361 g         1183 t
ORIGIN      
        1 ttccacatat agaagtgata tttatccatc aggggctgag tgatattctg cgggaggatg
       61 caaaacagag cgttcacgac cggcggcatg gtgatttcta tctgatcaag ctctgccaga
      121 cgctctccca gaagctcgca catccggttg gcatgcaaag cgttggccct ccacagctca
      181 ttctccagat aggcgataaa ctgcgccccg ataaagcgca tcttgctgaa aagctgggaa
      241 gcctgtttgc ggtaaaagcg catggcattg ttgatctcag gccggaagct gatgatcgcc
      301 tcgccaaaga gcaggccgtt tttggtgccg ccaaagctca cgatatcggc tccggcatct
      361 ctggtaatag ctgccagaga gcagtttaga gcagccgcgg cattggccag ccgggctccg
      421 tcgaggtgca gatacatgtc atgttgatgg gcaaaatcgg ccagcgcgga gatctcattg
      481 ggagtataga gactgccgta ttcggtgctt tgggagatgg aaatcactct gaactggctg
      541 tgatgctggt cccggttgcc atataagcgt ggcgcgatca gttctggagt cagcttgccg
      601 tccggggtgt cgatggcctg cagacgtccc tgagtaaact tttgcacggc cccgcattca
      661 tccacgttga tatgagcgct actgggacag gccaccgcgt gccatgagga gataagagac
      721 tgcagggcta tcacattggc cccggtacca ctcattacga accaggcctc gcaagccccg
      781 ccaaagagct cgcgaacccg cgccagggct tcctgggtga tgggatcgtc gccataggcg
      841 ggacaataac cctgattggc cttgaggatg ctgtccatga cggcgggatg aactccgctg
      901 tggttgtcgc tgccaaaact gatgggaaac atagtatata tcctttgtcg ttatacgtta
      961 tgcgttaacc gttatgcgtt atgcgttacg cgttatgggt ttggagtacg tggttttgcc
     1021 tttcgggctt ccagtttacg ctgcaggcgc tccagcctcc tggcagcttc ggacaggcgt
     1081 ttcaggtccg gcaccggtct gctctgatgc cagttgatga gggtttgggt cctgtggacg
     1141 gccgtttcca gctctcccct cttttccagg atcatacaca gctccagcag ggaggctggg
     1201 tgttccagat cggaggcgat ctcaaagcac tcactggccc cttccaaatc gccttcccgc
     1261 ttataaacca tgcccagttc ataggctgaa ggcccgctgc aaaagccctg ggccatgagg
     1321 tccaccagaa ttctcctggc cgtctcgtgc tggccctggc tctgatagag cctggccaca
     1381 gccagataat ccactctggg atcctggcca gtcttcacag gctcagcgat ggcgttgccg
     1441 atgatgccaa acagggccgc ggtgttgagc acgtccaact ggttgtgcaa gagaatcctg
     1501 cggatgggtt ccggatcgcc gctgatcaga tactgataat aggtctgggg gatcaggcca
     1561 ccgctgatgt ccagttcctt gtctctgatc tggcccatga tatagaattc caggctttcc
     1621 agagcgcagg agggaagctg gtttttccag agccgccggg ctatatgcaa gaggtccaga
     1681 tgctcttttt cccgcagatt gagccacagt ctttgataga ggaggcggga ttccagcacc
     1741 gggatatcga aggtcttgcc attgaaggtg accagcacgc tgtgagtttc cagcagttcc
     1801 atcagacggt caaaggaatt gagctcggca tcaggatccg gcaggaagat ctgctccacc
     1861 gtgtaggtat cgccttcgta gtagcccaga ccgatcagaa aggcgagggt gttgcctctg
     1921 cccagaccgg tggtctcgag gtccaacacc agaaagtctt ccggtttcca gggggtggaa
     1981 aggtccatgc cggcccagct taacagcact tccggcaagg cgttgggata caggggctgc
     2041 ccatcgtcgc taaagcgctc ccagctcttt ttgatcagat agaccgggtc tctggaatgg
     2101 acggaccact ccccctccac cattgcctgc agggcttcac gggtgagagt cttgatatcc
     2161 aggctgtagt cttcccggtc gcggtccata tccatggtgc aaggctggca ggggtggatg
     2221 gcttgtcaat aaaaagctga gatggactgg tgagggctat caattcactg gcgcatagtt
     2281 tatccttctg tccggggtgg attgtggaaa gatatccata gcgtcgtgca taaatttcct
     2341 cattttctaa tgattatcgc ttgacaggaa gcggggaaag aattgaccat tgctctgtgt
     2401 gttgattctc attatagcaa taccctggga gtatagtaat gcaagaagcg aaagacggcc
     2461 ggattttcag ctccgaaaca gaacacaggg agtttgtgcc ctggtggcgt tctggcaccg
     2521 gcatcgtctt tttgatcatc ctgctcttct ttgtctatgc ggccgccctg atcattccaa
     2581 ggctcagcag cacccgaaca gtgcttgtga acggcctgga agtgttgagg gacatcaaac
     2641 cgacccgcat tctgatcagc cccaaagaag ccagacaaat ggcccggctt catctggaaa
     2701 agctgattgg cgcagccccg ggggcagagc ttcgggaata tgccaggccc tggagcagcg
     2761 atcctgtctc gcgttattac gccctcttcg aaaacagcat cgatgccttt tataatcttc
     2821 tggatagcta catcatccgc gagggctgga gagtggactt tttggacacc cgagacaaca
     2881 gctttccctg gagcgtgttc ctggattcat ccggaaagct gctctgctgc cagaacaatc
     2941 ccacccatcc tgacaaaccc ggggccatca cagaagccga ggcccgtctg accgcacaaa
     3001 gctatctgac tcccacggtt tatggctcca ttcccagctt tcatgaaagc tcggctgtcc
     3061 cgcttcccga tcagcatcag tggctcttca gcttttctga ttctctggat cacaagctgg
     3121 tttccggccg ggttcagctg cagttggtga ttggtgctgg aggagttacg tccttccagc
     3181 ccggaatcag catgccccca gaccacgccg cttctatagc cgccgctgag cagctcacca
     3241 ggcggatgaa gattggggcc ctggtggtca ttctgctctt catgatcagg ctgtttgtct
     3301 ttaccctggg aaggttcagg aggggtgaat gggaccgcgg caccttctgg aggtctctgc
     3361 tgctcttgct tcccatgatc ctctacaata tgtatgatcg caatcatcag ttgcgtctgt
     3421 gggtcagcga accgggggtt cctgtcataa gcggagcggt tttttatgga gcgggagcgc
     3481 tcttcgcgct cttcatctca atgtttctgg cctggagcct caagtggatg cgcgaacgtg
     3541 agattgatcc gcactacatg cccctcgtgg ctggccgggt cccgctcatc aacctcagtt
     3601 cctttctcag cctgctggga gtgatcctga tggtcgtcaa tatgggcctg gcccacatgt
     3661 tttccggacc ctcggaagcc ctgcccatct acagcaaccg tatctcactg tcttttatca
     3721 tgtttgccat gtatttgctg atcctgggat tcatgatctt tatcgtgtgg cgctgggaac
     3781 agatcaggcg tcggaccgcg ccccggatca tagcaggctt agtggcgttc ctggtcatca
     3841 caagtatcat taccctcagc actttaccgt tgtttaaggg acccttccag gccagactgc
     3901 tgatcatgtt cttctgtttc atccccttta ccgggatcac ggcgctcctg ctgctctatc
     3961 agatgaggat caggatgtgg tatctatgca gtctcacatg gttggcggca ccagttctct
     4021 gcgagttctt atctcccagc cataaatatg gaggagaaaa caccttcatc atcgtggccc
     4081 tcgtcgctct gtatggctgg attctgatcg tgctccccga gcggaaacgg aaacaaaccc
     4141 cttgaccaga tctgcccctt atctccaata gtatatcctc tcttgaggat caagatacaa
     4201 aaaacaggga gtttgcgtga aaaagattct gatcgtgatt gggatcctgt tggtggcttt
     4261 tttcatctac agggagttca ttggcatcaa gcaattaacc ggtgaaaatg acgagtataa
     4321 cctcaagctc gccattgagg accatattga tccctattgg ctggaggccg gtgctggagt
     4381 tgagaagatt tatgtggata agctggagga tgtaaagtgg gagaccatcg acaaagatca
     4441 ggggctatac gactgctcgg caactatcaa gggcacgtat atggtgaggg gatcaaaaca
     4501 agccttttcc gtgcgccgca agttcaacgt cgatcgcagc aacaagatta agggctatcg
     4561 ggtcaagctg atcgaatgag ggcgttcata actgattcag gacggggtga ttgaccgcgc
     4621 gagcggcttt agaccaggta tgagaaccgg caaaatcatc gccatcgagt gtggctgcgg
     4681 ctacctgctg ttccgctatt acaaagctgg aaagggacgc ctgatcaaat gcctgatcag
     4741 ccgcctcagc caggataatg tgggcctcca gggtgccgcc actctctcca gaccattgtg
     4801 tccatcctgt ggcaaagaac tgggcatcat catgatgatc cacggcgaac cggctctgaa
     4861 actgaaccaa gggacgatca ggagcgtgag agtgtagaca ggatg
//
LOCUS       DAOSFN010000163         4800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_15813_length_4800_cov_9.764594, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000163 DAOSFN010000000
VERSION     DAOSFN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15813_length_4800_cov_9.764594"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..795
                     /locus_tag="PLO35_08785"
     CDS             301..795
                     /locus_tag="PLO35_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79804.1"
                     /translation="MKTILALFGLFFLCTALPAATWNVILIDSYGNGWNGATLTVMVN
                     GTAVLNNITLASGSEQSYTLAIFHGDKITTTYVAGIYPGEPEYSFANEVGVMVLSNGA
                     GPTVVPTSITDPFMAAVLTPDLYQIGAGTLTGFLPIIPYFGYSYSQSIYRRSDLPRTW
                     KATA"
     gene            945..3059
                     /locus_tag="PLO35_08790"
     CDS             945..3059
                     /locus_tag="PLO35_08790"
                     /inference="COORDINATES: protein motif:HMM:NF025697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HOH79805.1"
                     /translation="MNLLASPGWVEIVLDRPFNYDGTSNLVVAVDENTDLYDAYDCRF
                     LEYGGATNRGLFFYSDITNPNPASPPAAMAQESIIPNIIFQMDPVLYPPCGESFDLPA
                     VPPAWAQYRSANIGMDLWSTSPTNYAGGNSPELQSIWVSVIGTSRLISPPVQTAGLSS
                     LELEFKHYFDDYGTGVTGKLQYSHDMNTWLDSGWSFDSGAGDISGTVTLPLSGLVGNV
                     TYLAWVLEGDLYYFDRWYLDDVSVNIPLIDVAPLRIDTPEVVDFDTFTPQATFINNGS
                     TTADFAVTFTLGDAAYVNTQTVTGLAPGATQTVDFANALGWVNFTMPMRATTHLVNDQ
                     VSANDQIGKDLTSLILDKQVYADIYDYNYPPTRKGPSTFYLKTPGTLTHLGAPSVTNV
                     FMAGGEWIGNAWFGTEYDTDSLAYDNYYRIDHHTGVQTLLGQTGVPIHGLAWDSNHNI
                     LYGTNLTSLYMLNQATGAATLLGPHVNTPLMIGLAYDSYTDTLYGVDLSTDALYSIDT
                     STGLATQVGLLGIDINLGQDLAFDREHGYLFLAGYNMGSDPGPHLYWIDQSTGLAHKI
                     GDFPEDTQVVGFAIPSEPLVLNLRIAANGVLSWDPVPGVTEYRIYGANYPYATTYTHL
                     ANTSSTSWLDPIFPQPMRFYYVSAAYTRSDAEPVVVRYLTEYKDPLTGELLDPQPVVE
                     PAAAQPLPAVNSGENSAGLARP"
     gene            complement(3191..3964)
                     /locus_tag="PLO35_08795"
     CDS             complement(3191..3964)
                     /locus_tag="PLO35_08795"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOH79806.1"
                     /translation="MKTIRNILAICLLLGSVYASATVLNVPQIYQTQTQWCWAATSQA
                     MLQYYGYYFTQNEIAQYGTDGANEWNWLWGSTTNPTRRGIDLIMQYFANLSTSPYGRS
                     LSLAESASFIDADRPFYVRWGWDSGGGHFLVAHGVESSTMYLMDPWYGPTINSYAWTL
                     SGGGHTWTHSLTMNTAPYLEPVTALQITSSLSTVQLSWNPVIRANSYLVYSAAQPDPA
                     DWGTPCAIVSGTGCTLDAGTSGKGFYRVVASSDLPPARD"
     gene            complement(3966..4616)
                     /locus_tag="PLO35_08800"
     CDS             complement(3966..4616)
                     /locus_tag="PLO35_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79807.1"
                     /translation="MKKTIVFVFLFLCGYTFVYTTPVFGEINPDFAFQEADKALPILG
                     LDQFLGMIDEQNFQLYGFSEIGDVRQVKPGLPLQLFTIKPQSILNYTSGATTEELLEP
                     TNQCYIPLLSQGKPQCMVLATIAADGALQVVSLGYAKLADQLYAAFSGADLNPELSRL
                     VVVYQAREFFLSDQSKPDRLYPIQNTYEAGLNADTNVEHNLSRIRAGVEAALQEGR"
BASE COUNT         1179 a         1335 c         1188 g         1098 t
ORIGIN      
        1 tgccgatttg aaaaaatggt gatacgattt gaaaaaatag ttgagatccc ataaccagca
       61 aatgaatctg aagcccataa accagagcca gttaaacaaa aagcttgaca gataattcac
      121 caatatatat cttgagaatt gatcgcatag gtctgctctc ccataccggt ggagtcccag
      181 ggaggtggaa atggacctca gcagcgccag tatttcggaa ttcttgttgg ctgtccttaa
      241 aacaaaagtg ccccacccag gtttgatcac aatcaacagc tcaaataaag gagttttacc
      301 atgaaaacaa ttcttgcctt atttggcctc tttttccttt gcacagccct cccggccgcc
      361 acttggaacg taatcttgat cgacagctat ggcaatggct ggaacggagc cacgctcacc
      421 gtgatggtca atggcaccgc agtcctgaac aatatcaccc tggcctctgg cagcgagcag
      481 agttataccc tggctatctt ccatggcgat aagatcacca ccacctatgt tgcaggaatc
      541 tatcccggcg agccggaata cagctttgcc aatgaggttg gagtgatggt cctcagcaac
      601 ggcgccggcc ccaccgtggt ccccaccagc atcacggatc ccttcatggc cgcagtcctc
      661 accccagatc tttaccagat cggggcagga acgttgaccg ggttcttacc gatcattccc
      721 tattttggct acagctattc ccaaagcatt taccggcgct cggatttgcc gcggacctgg
      781 aaagccaccg cataagcaag atcaaatacc aatggaacgg cctggccagc gctacccacg
      841 ccctcaattg gacagtatat atcggtcaca tcacgcggac gcaattcacc tcgactactg
      901 attgggttcc gctcgcggag ctcacccagg tctttcaggg cagcgtgaac ctgcttgcca
      961 gtcccggctg ggtggagatc gttttggacc ggccgttcaa ctatgacgga accagcaatc
     1021 tggtggtggc ggtggacgag aacacggatt tatacgatgc ctacgattgc agattcctgg
     1081 aatatggtgg ggctaccaat cgcggcctgt ttttttacag cgatattaca aatccaaatc
     1141 cggcttcacc gccggccgcc atggctcagg aatcgataat tcccaatatc atattccaga
     1201 tggatccggt gctgtatccg ccctgtggcg agagttttga cctgccagcc gtcccaccgg
     1261 cctgggcaca ataccgctca gcaaacattg gcatggacct gtggtccact tcccccacaa
     1321 attacgccgg ggggaacagc cccgaactgc aatccatctg ggtttccgta ataggaacca
     1381 gccggctgat ctcaccgccg gtgcagactg ctggcctttc ctctctggag cttgaattta
     1441 agcattattt cgacgattat ggcaccgggg tgaccggaaa actgcagtac agccatgaca
     1501 tgaacacctg gctggactcc ggctggtctt tcgacagcgg agcaggggat atcagcggaa
     1561 cggtgacctt gcctctatca ggcctggtgg gaaatgtcac ctatctggcc tgggtgctgg
     1621 aaggagacct ctattatttc gatcgctggt atctggacga tgtgtccgtc aacatacccc
     1681 tgatcgatgt agcaccgctg agaatcgata ccccggaagt ggtcgatttt gatactttca
     1741 cgccccaagc cactttcatc aacaacggta gcaccacagc agattttgcc gtgaccttca
     1801 ctctcgggga cgccgcttac gtcaacaccc aaactgtcac agggctcgcc ccgggcgcca
     1861 cgcagacagt tgatttcgcc aatgcactag gatgggtgaa cttcaccatg cctatgcgcg
     1921 ccaccaccca cctggttaat gaccaggtat ccgccaacga ccagatcgga aaagacctga
     1981 cctcgcttat actggataag caggtctacg cggatatcta tgattacaat tatcccccaa
     2041 ccaggaaagg gccttccacc ttctatctga agactcccgg cacactcacg cacctgggcg
     2101 cgccgtccgt cacaaatgtg ttcatggcag gaggggagtg gatcggcaat gcctggtttg
     2161 gcacggagta tgacaccgac agcctggcct atgacaatta ttaccggatc gaccaccata
     2221 cgggagtgca gaccctgctc ggtcaaaccg gagtgcccat ccatggactg gcgtgggatt
     2281 ccaaccacaa tatcctctac gggacaaatc tgaccagcct ctatatgctc aaccaggcca
     2341 ccggcgcagc taccctgctt ggcccgcatg taaatacccc tttgatgatc gggcttgcct
     2401 atgatagtta cacagacact ctttatggcg tggacctgag tacggatgcg ctctattcca
     2461 tcgatacttc caccggcctg gccacgcaag tggggcttct gggcatcgat ataaacctgg
     2521 ggcaggacct ggctttcgac cgggagcacg gctacctgtt tttggcaggc tacaacatgg
     2581 gttcagaccc aggtccccac ttatattgga tcgatcagag taccggcctc gctcacaaaa
     2641 tcggtgactt cccggaagac acacaagtgg tgggctttgc catacccagc gagcctttgg
     2701 tgctcaatct gcgcatagcc gccaatggag tcctcagctg ggatccggta cccggggtga
     2761 cagaatacag aatctatggt gccaattatc cctatgccac tacctatacc catctggcca
     2821 acacctcaag cacttcctgg ctggatccaa tctttccgca acccatgcgc ttctactatg
     2881 tgagcgccgc atacacccgc tctgacgcgg aacctgtcgt ggtgcgctat ctgaccgagt
     2941 ataaagatcc tctcaccgga gagctccttg atccgcaacc ggtggtggaa cctgcggctg
     3001 cccaaccgct tccggctgta aatagtggag aaaacagcgc aggactggca cggccttaga
     3061 aagtaaaata agacccggcc aacctgaggt agactcagac aggtttaggg gactactcaa
     3121 gcttgctgtg tgaacttcgc tcaagctccc cagcctgcta acaaggacat cgatcaggta
     3181 ttccgctggt tcaatccctt gctggcggca gatctgacga tgcaaccacc cgatagaatc
     3241 cctttcccga agtgcctgcg tccaaggtgc agccggtacc gctcacaata gcacagggag
     3301 tcccccaatc cgcaggatca ggctgagctg cagaatacac caggtacgag ttcgctctga
     3361 taaccgggtt ccaagacagc tgcacggtag acagtgaact agtgatttga agcgctgtta
     3421 ccggttccag gtagggtgct gtgttcatgg tcaggctgtg tgtccaggtg tgtccacctc
     3481 cgctgagggt ccaagcgtag gaattaattg tggggccata ccagggatcc atcaggtaca
     3541 tcgtactact ttccacccca tgagccacca aaaaatggcc accgccggaa tcccatcccc
     3601 agcgtacata gaaaggccgg tcggcatcga taaaggaagc tgattctgcc aaactgaggc
     3661 tgcggccata aggagaagtg ctcagattgg caaaatactg cataatcaga tctattcccc
     3721 tgcgggtcgg attggtggtt gatccccaca accagttcca ttcgttcgcg ccatctgtcc
     3781 catactgggc tatttcattt tgggtgaagt aatagccata atactgcaac atggcttgag
     3841 aagtggcagc ccagcaccat tgagtttggg tctgatagat ctgaggcacg ttcaagactg
     3901 ttgcagatgc gtacaccgag ccaagcagca ggcagatagc gaggatattc ctaatcgttt
     3961 tcatgttaac gtccttcttg cagggctgct tccacgccgg ccctgattct gctgaggttg
     4021 tgttccacgt ttgtatctgc gttaagacct gcctcgtagg tattctggat tgggtataag
     4081 cgatccggct tggattgatc ggaaagaaag aattcccggg cttggtaaac gactaccaag
     4141 cgggaaagtt ccggattaag atctgcacca ctgaatgcag catagagctg gtctgccaat
     4201 ttggcgtagc ccagggacac cacttggaga gcgccatccg ctgcgatcgt tgccagtacc
     4261 atacattgcg gcttaccctg gctcaacaga ggaatatagc attgattggt tggctcaagc
     4321 agttcctccg tggtggcacc gctggtgtag ttcaggatgc tctgcggctt tatcgtgaaa
     4381 agctgaaggg gaagacccgg tttgacctgg cgcacatctc caatctctga aaagccataa
     4441 agttgaaagt tttgctcatc gatcattccc aaaaactgat ccaagccaag aatcggcaga
     4501 gccttgtcag cctcctgaaa agcaaaatcc gggttgattt ccccgaaaac aggagtggta
     4561 tacacaaagg tgtagccaca taagaaaaga aatacaaaaa cgatggtctt tttcatgatc
     4621 tctcctactt atctgtgaaa ttccgagctt tttctacgat agctcattcc tacgttaatt
     4681 ctatacgacg ggctttccct gtcaagaaaa atatcgggat tttaaccgga atggcgaaaa
     4741 ataagtgcaa accctcactt aaaacttcaa attgctctca cttggacaat tgaaggtatc
//
LOCUS       DAOSFN010000164         4687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_16356_length_4687_cov_8.549439, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000164 DAOSFN010000000
VERSION     DAOSFN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16356_length_4687_cov_8.549439"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLO35_08805"
     CDS             complement(<1..403)
                     /locus_tag="PLO35_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79808.1"
                     /translation="MNTKLLLFCLLVLGVCSHATILSVALDGSQPYSSIQTAINASAH
                     TDTVLVYPGRYYENVRFNGRNITLASLELMTGNRDYVYSTIIDANHNGTGIYVNDYET
                     NVTIQGFSVVNGTGYYNSQYDWTSGGGIAIGG"
     gene            838..2271
                     /locus_tag="PLO35_08810"
     CDS             838..2271
                     /locus_tag="PLO35_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79809.1"
                     /translation="MDKRFIRPLLIVLLAVLLLVFIGPSIPVWENEILPIKPMDIISS
                     VLAAPDTTSVSQEAVKLAEQAPTVEELGPLDQFAAKLQALKSGKNQQLRIAYFGDSII
                     EGDLVSGRLRKNLQDFAGGNGVGLVGMTSIVNKFRKTIIHDFSTNWEAVSFMSKSSTP
                     LGIMGHTFIPRSWQNVETTIIPTAESSSTDTLASDSTSAKPVAVPKKVSRKVSVSGPA
                     WVQYQGTDIPGGAASFEHIRLFYSQALENSSVRVIYDGGEARTVSLSPGNDVQVLDLS
                     PAAPVKKLRLEFSSGHPIHVYGVSFDAPTGVYVDNISIRGYSGMYFNRIPGDLLRGFQ
                     RALNYDLVILQYGENVSNARVTDYDFYRKGMVRSIKHLQDAMPGVPFLIISAHDRSIR
                     KDMGYSTSPDIPILIKAQSEAAQETGSAFWNLFAEMGGMNSMPSWVNASPALAGKDYT
                     HFTIAGANKVGEMLSNLILSGSKAVSE"
     gene            2268..3191
                     /locus_tag="PLO35_08815"
     CDS             2268..3191
                     /locus_tag="PLO35_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOH79810.1"
                     /translation="MKGLRLLLSIAVLSSLGFHGLIAISEPLPVAVEDEIEIISEFPV
                     EVDSLEEFIQSYYELLDSETYLKEFKFLDQKANHLDNHKQLLAPLFGKLMALRNGSTE
                     RVSIYQIGDSHIKPGYFSTTVRGSLLNFFQPGVPIDAAAIQYHFTGINGASFSNLTPN
                     DAIFEKIKALQPDLLIVSLGTNDAQGNYVASRFRAEMQGFMTRLLAAQPEPKLIFTLP
                     GDSSKNNRHNSNVAKVCSEIKDYARDHNFAWWDLFEVMGGSKSITKWKAKDLASQDLI
                     HYSPKGYMLQGYLFYSALIRAYKDLAEGGSQ"
     gene            3188..4669
                     /locus_tag="PLO35_08820"
     CDS             3188..4669
                     /locus_tag="PLO35_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004371558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HOH79811.1"
                     /translation="MTAWQNLLSKLLYDPAVPLFFNSGFFLFFFLIVMIFYPLLVKRV
                     QVRTWYLMLISLYFYYKTSGLFVVLLVLTALINFLLGAGISKTEKRGKKRALMWLSVI
                     WNLGSLGYFKYTNFIIDSINQLSGGNLPLMDIFLPVGISFFTFQTMSYTMDIYFGKLT
                     PLKSFKDFVFFVAFFPQLVAGPIVRASWFIPQIRKQLELNKDQIAGALVLIFAGLIKK
                     GVIADYISINFVDRVFDSPELFSGVENLLAVYGYGLQIYCDFSGYSDIAIGLATLMGF
                     NLPVNFNVPYVATSITDFWRRWHISLSTWLRDYLYIPLGGNRKGVIRTYINLMITMLL
                     GGLWHGASWNFVVWGGLHGVALAIDKLRMGLFGTRDSGSGIGHYLAKIFGWLITFNFV
                     SFTWIFFRARGFEAAWTVISRIIYDFNAPIFWQWISGYQLVAILIAYGYLIHFIPHSV
                     DRGFEWLLQRTPLVFQSVLLACVIWVLFQARSADIQPFIYFQF"
BASE COUNT         1070 a         1322 c         1156 g         1139 t
ORIGIN      
        1 tccctccgat agcgattcct ccaccagaag tccaatcata ttgcgagttg tagtatcctg
       61 taccgttaac cacagagaac ccttgtattg taacgtttgt ttcataatca tttacataga
      121 tgcctgttcc attatggttt gcgtcgatta tggtggaata cacgtaatcc cggttccctg
      181 tcatcaactc caagctggcc agagtgatgt tcctgccatt gaaacgaacg ttctcgtaat
      241 atctgcctgg atacaccagc acagtgtctg tgtgagcgga agcgttgatc gcggtttgga
      301 tagaggagta gggctgcgaa ccgtccaggg ccacggacag aatggtggcg tgggagcaaa
      361 cacccaaaac cagcaaacag aataggagaa gcttagtgtt catagctcaa acaccgaagt
      421 aaaattcccg ccataaaaaa ctatgctttt tcccctgtca gaccttttgc ccaaactggg
      481 tgacaccaaa tacatcatgt ttatctcctc ttggccccgc gggccctggg atgctgtgct
      541 atcgacctca tggattataa aaaacgctgt catggactga attcatgatg gagttaatca
      601 ctttctgatc ccggagttgc tgtcaatgtt tttcttgaat gcgcagtaat caaacaagta
      661 atgcatccac ctcaccctaa gcggcaagaa tttccttgtc caaaaagccc tgttcagata
      721 tcaggctcca gattgataat aatagagata acaacattgt gaaacaacat cttatgacat
      781 acaatcccgt cacttcagat gccgtgccag ctcacgccat aaagagtcgc gcccaaaatg
      841 gataagcgtt ttatcaggcc cctgctgatc gttttgctgg cggtcttgct gctggtgttt
      901 atcggtccct ccatccccgt ctgggaaaac gagatcctcc ccatcaaacc catggatatc
      961 atcagttccg tactggccgc cccggacacc acgtctgtat cccaggaagc tgtaaagctg
     1021 gctgaacagg cccccacggt cgaagagctg ggtcccctgg accagtttgc cgccaaactg
     1081 caagccctca aatccggaaa aaatcagcag ctacgcattg cctattttgg cgattccatc
     1141 atcgaaggcg atctggtctc cgggcggctg agaaagaatc ttcaggattt tgccggggga
     1201 aacggagtcg gcctggtggg catgacctcc atcgtcaaca aattccgcaa gaccatcatc
     1261 cacgactttt ccaccaactg ggaagccgtt tcctttatga gcaaatccag cactcccttg
     1321 ggaatcatgg gccacacctt catcccacgc tcctggcaga atgtggagac caccataatc
     1381 cccaccgctg aaagcagttc cacggacact ttggcatcgg acagcacgtc cgcaaagccg
     1441 gtggccgttc ccaaaaaggt gagccgcaaa gtctctgtga gcggaccggc ctgggtgcag
     1501 tatcagggaa cagacatacc tggcggggca gcgagttttg aacacatccg ccttttctac
     1561 agccaggcgt tggaaaactc cagcgtccgc gtgatctatg acggcggtga agccaggacc
     1621 gtgagcttga gtcccggaaa cgatgtgcag gtgctggacc tcagccctgc cgctccagtc
     1681 aaaaagctac gtctggagtt ttccagtggc catcccatcc atgtctatgg agtcagtttt
     1741 gacgctccca caggcgttta cgtggacaac atttctatcc gtggatattc cggcatgtat
     1801 ttcaaccgca tccccggcga cctgttgagg ggctttcaac gcgccctgaa ctatgatctg
     1861 gtgatcctgc agtatggcga aaacgtctcc aacgccagag tcacggatta cgatttttac
     1921 cgcaagggta tggtccgcag catcaaacat ctgcaggacg ccatgccggg cgtgccgttc
     1981 ctgatcatca gcgctcatga ccgcagcatc agaaaggaca tgggctattc cacctcgcct
     2041 gacatcccga tcctgatcaa ggcccagagc gaagcggccc aggaaaccgg cagcgcgttt
     2101 tggaatctgt ttgccgagat gggtggcatg aattccatgc cctcctgggt aaacgccagt
     2161 cccgccctgg ctggcaagga ctatactcat ttcacgatcg caggcgccaa caaggtgggc
     2221 gaaatgctct ccaacctcat cctgagcggc tcgaaggcag tgtcagagtg aaaggcctca
     2281 gactcctgct cagcatcgcg gtgctctcca gcctgggatt tcacggcctc atcgccattt
     2341 cggaacccct accggtggcc gtggaggacg aaatcgagat tatcagcgag tttcccgtgg
     2401 aggtggacag cctggaagaa tttatccaga gttattacga gctgctggac agcgagacct
     2461 atctgaagga atttaagttt ctggaccaga aggccaacca tctggacaac cacaaacagc
     2521 tattagcccc cctgtttggc aaactgatgg cgctccgcaa cggttccact gagcgcgtaa
     2581 gcatttacca gataggcgat tcccatatca aaccgggata tttctccacc accgtgagag
     2641 gctcgctgct caacttcttc cagcccggtg ttccgattga cgcggccgcc atccagtatc
     2701 actttaccgg catcaatggt gcctctttta gcaatctcac ccccaacgac gccatcttcg
     2761 aaaagatcaa agcgctgcag ccggacctgt tgatcgtgtc tttgggcacc aatgacgctc
     2821 agggcaacta tgtggcctcg cgtttcagag ctgaaatgca aggttttatg accaggctgc
     2881 tggccgctca gccagagccc aaactgatct ttaccctccc gggtgattcc agtaaaaaca
     2941 acagacacaa ctccaatgtg gccaaagtct gctccgagat caaagattat gcccgcgacc
     3001 acaactttgc ctggtgggac ctctttgagg tgatgggcgg cagcaaatcg attactaaat
     3061 ggaaggccaa agaccttgcc tcccaagacc ttatccacta tagccccaaa ggttatatgc
     3121 tgcaaggata cctgttttac agtgccctga tccgggccta caaagatttg gctgagggcg
     3181 ggtctcaatg acggcgtggc aaaacctgct ttccaaactg ctctacgatc cggccgtacc
     3241 actctttttc aacagcggct ttttcctctt tttctttttg atcgtgatga tcttctaccc
     3301 cctgctggtg aagcgggtgc aggtgcgcac ctggtatctg atgctgattt cgctgtattt
     3361 ttattacaag accagcggac tttttgtggt cctgctggta ctcacggccc tgatcaactt
     3421 cctgctggga gcgggaatca gcaagacgga aaagcggggc aaaaaacgcg cgctgatgtg
     3481 gctgagcgtg atctggaacc tgggctcgct cggatacttt aaatacacca atttcatcat
     3541 cgattccatc aatcagctca gcggtggtaa cctgcccctg atggatattt tcctgcccgt
     3601 ggggatctcc ttcttcacct tccagacgat gagctacacg atggatatct acttcggcaa
     3661 gctcacgccg ttaaagagct ttaaagactt cgtgtttttc gtggcttttt tcccccaact
     3721 ggtagcgggg cccatcgtga gggcctcctg gtttatccct cagatccgga aacaactgga
     3781 gctcaacaaa gaccagatcg ccggagcgct tgtcctgatt ttcgccggcc tcatcaagaa
     3841 aggcgtgatc gcggattata tcagcatcaa ctttgtggac cgcgtcttcg acagtccgga
     3901 gctcttctcc ggagtggaaa acctcctggc cgtgtatggt tacggactgc agatctactg
     3961 cgacttctcc ggctattccg atatcgctat cggtctcgcc accctgatgg gcttcaacct
     4021 gcccgtcaac ttcaacgtgc cctatgtggc tacctccatc accgatttct ggcgccgctg
     4081 gcatatctcg ctttccacct ggctgcggga ttatctctat atccccctgg ggggcaatcg
     4141 caaaggcgtt atccgcacct acatcaacct gatgatcacg atgctgctgg gaggcctctg
     4201 gcatggggcc agctggaact ttgtggtctg gggcggcctg catggagtgg ccctggccat
     4261 cgacaaactg cggatgggcc tcttcggtac acgggattct ggctcaggaa ttggacatta
     4321 tctagctaaa atcttcggct ggctgatcac cttcaacttc gtgagcttca cctggatctt
     4381 cttccgcgcc agaggtttcg aagctgcctg gacggtgatc agcagaatca tttacgattt
     4441 taacgcgccg attttctggc agtggatttc aggctatcaa ctggtggcca tcctgatcgc
     4501 ctatggctat ctgatccact ttatccccca cagtgtggac cgcggctttg aatggctact
     4561 gcagcgcacc ccgctggtgt ttcaatctgt cttgctggcc tgtgtgatct gggtactgtt
     4621 ccaggcccgc agcgcggata ttcagccctt tatctatttt caattctaga ggttacgcgt
     4681 gatgcgt
//
LOCUS       DAOSFN010000165         4648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_16528_length_4648_cov_9.096669, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000165 DAOSFN010000000
VERSION     DAOSFN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16528_length_4648_cov_9.096669"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1205)
                     /locus_tag="PLO35_08825"
     CDS             complement(180..1205)
                     /locus_tag="PLO35_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOH79812.1"
                     /translation="MKSRQITDRLSTEAARLFGAVGTPVYIAGGHEWSDGTVYQASHP
                     LGALMLKLIPDASQENILGLIERLEFFDYLGARGIQVARPLHSVNGAVIETVAEDEAI
                     FIARAWTRIPGEHLPDQHPKDLSDYYHAWGTMLGKLHRLAREHPQWRHSACLDGSGAP
                     NLSWQREWELFFHWLKGDGVKKAWEGIRAELERLPVSRDNFGFIHNDPHPGNILVNES
                     GLALLDPEVANYHWFMTDLGICLNSEHSRIGHHSKHKEALADLPDLFLRPFMQGYHSQ
                     NRLPADEYHRIRLFLRYRRFLMYAVFYEQIKQHDPDYLKGFTRELIDGKNPAADLMET
                     EFIRLSR"
     gene            1341..1712
                     /locus_tag="PLO35_08830"
     CDS             1341..1712
                     /locus_tag="PLO35_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOH79813.1"
                     /translation="MQVGAFSISLSVKDIHASQNFYRKLGFEVLGGDIDQNWLILKNG
                     ECVIGLFEGMFEGNILTFNPGWDQNAQPLESFTDIRCLREDLERLEMNPGPLDPNTVQ
                     GPASFTLSDPDGNLILFDQHR"
     gene            complement(1827..2045)
                     /locus_tag="PLO35_08835"
     CDS             complement(1827..2045)
                     /locus_tag="PLO35_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH79814.1"
                     /translation="MLDHSAGITNTIRKCRFDQGEMTQEQLAQKVGVTRQTIIALEAG
                     KYLPSLLLAIRIARVFDKNVEEVFQIGT"
     gene            complement(2032..2451)
                     /locus_tag="PLO35_08840"
     CDS             complement(2032..2451)
                     /locus_tag="PLO35_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79815.1"
                     /translation="MVIQQKESGLTLAGSLAFIAIRLLIPRSGKTGEVELTLLLLVCF
                     IFMLWIVRHFTGARLKALDEMDLTIRSQAAIVGAHGFGAVVMIFAVLLYLMHRGSGFV
                     PLDQVINLAYYSWLSLYIFWSGAILILYKTGAWHVGS"
     gene            complement(2678..3820)
                     /locus_tag="PLO35_08845"
     CDS             complement(2678..3820)
                     /locus_tag="PLO35_08845"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOH79816.1"
                     /translation="MLIKMPQLKIGDLIAKLPIIQGGMGVGISLSRLAAAVANEGGIG
                     IISSVGLGILHPQPGKSYKDANAAALKQEIRAARQKTKGIIGVNIMLAISDFDEMINA
                     AFEEEADIVFLGAGLPLKVPSTMTLEYLQNTKTKIGVIVSSGRAAKIILSHWAEHFNR
                     IPDIVVLEGPKAGGHLGFKEEQIFDEDYSLEKVFPQVKEAVREIENKYSKSIPVIAAG
                     GIFTGEQIYRIMELGADGVQMGTRFVATQECDADPAFKQLFVDCREEEIVIIKSPVGL
                     PGRAINNKFLRDVSAGVKMPIICPWKCLKTCDYHVAPYCIALALNNARNGVMADGFAF
                     AGANAYLVKHITTVKELIRDLVQEYIDYRNKIMNIDQPVLVPVRND"
BASE COUNT         1103 a         1180 c         1163 g         1202 t
ORIGIN      
        1 ggtccagatc acctctcacc cacttcggag aaatggctct tggctcgggc ttggtagaga
       61 cgcgcgatac cttagcggtt tagagcgcta taagaactcg catggctttg atccccgcgg
      121 ctctccagaa catatcatac accgcctgta tgacatacgg ccggtcttcc ctgagctcat
      181 tagcgggaca agcggataaa ctcagtctcc atcaggtccg cggctgggtt ttttccatcg
      241 atcaactctc tggtgaagcc cttcagataa tccggatcat gttgtttgat ttgttcataa
      301 aaaacggcgt acatcagaaa gcggcgatat ctgagaaaga gtctgatcct gtgatactca
      361 tccgctggga gccggttctg actgtggtaa ccttgcatga atggccgcaa aaacaggtcc
      421 ggaagatcag ccagggcctc tttatgcttg ctgtgatgcc caatgcggga gtgttcgctg
      481 ttgagacaga tacccagatc ggtcataaac caatgataat tagccacttc aggatcgagg
      541 agcgccagcc cgctctcgtt gaccaggatg ttgccggggt ggggatcgtt gtggatgaag
      601 ccaaagttgt ctctggagac gggaagcctt tccagttcgg ctcgtatgcc ttcccaggct
      661 tttttgaccc cgtctccctt cagccagtga aaaaacagct cccattccct ctgccaggat
      721 aggttgggag cgccagagcc atccaagcag gcggaatgac gccattgagg atgctctctg
      781 gccaggcggt gtagtttccc gagcatggtc ccccaagcat gataataatc actgaggtct
      841 ttaggatgtt ggtctgggag atgctcaccg ggaatcctgg tccaggccct tgcgataaaa
      901 atggcctcat cttcagccac agtctcgatt accgcgccat tgacggaatg gaggggtctg
      961 gccacctgaa ttccacgggc tcccaggtag tcaaagaatt ccagcctctc aatcaggccg
     1021 agaatgtttt cctggctcgc gtccggaatc agcttcagca tgagcgctcc cagtggatgg
     1081 ctggcctggt aaaccgtccc atcagaccat tcatgccctc cggcaatgta aacaggggtc
     1141 ccgactgccc caaacagcct cgcggcttcc gtgctcaagc gatctgtgat ctgcctcgat
     1201 ttcattgttg ctccctaatc ctgatgggaa cagcttctct ggccaggctg gtaagtcaag
     1261 ggatactttg ccttgtttat aaaacttgac tgacgcggcc atcagaaatc atgggggaaa
     1321 aacgtgaatt aggagtcaaa atgcaagtgg gagctttttc tatcagtctg agcgtgaagg
     1381 atatccatgc ctcacaaaat ttctatcgta aactgggctt tgaggttctg ggcggtgata
     1441 ttgatcagaa ctggctgatc ctgaagaacg gcgagtgtgt gatcggcttg tttgagggca
     1501 tgtttgaggg taacatcctc accttcaatc ccggctggga ccaaaatgcc caaccccttg
     1561 agagctttac cgatatcagg tgtctgcgcg aagatcttga gcgtctggaa atgaaccccg
     1621 gtcccctcga tccaaacacc gtgcaaggac ctgccagctt cacactcagc gatccagacg
     1681 gcaatctcat tctctttgat cagcatcgtt aaacgggagg agtccgtagc ggccagaact
     1741 caggaggcga agctatccca atttctcgca atgcaaacca taaacacaag tttcacagcc
     1801 aattatgtga cgcaggacct gcccttttag gtaccgatct gaaacacttc ctccacattc
     1861 ttgtcaaaca ctctggcgat cctgatcgcc agcaacaggg acggaaggta ttttcccgcc
     1921 tcgagggcta taatggtttg acgcgtcacc cccactttct gagcgagctg ttcctgggtc
     1981 atttctccct gatcaaagcg gcatttcctg atcgtattgg tgattccggc actatgatcc
     2041 aacatgccag gccccagttt tgtagaggat tagtatggcc ccgctccaaa aaatgtacag
     2101 cgacagccag ctgtaatagg ctaggttaat cacctggtca agaggcacaa aaccgctgcc
     2161 ccggtgcatc agatagagca gtacggcaaa gatcatcacc accgctccaa aaccatgcgc
     2221 gcccacaatg gctgcctggg aacggatcgt gaggtccatc tcatccaagg ctttgagcct
     2281 ggcgccggta aaatgacgaa ctatccagag catgaagatg aagcacacca gtagcagcag
     2341 ggtgagctca acctctccgg tcttgccgct ccgcgggatt agcaacctga tcgcgatgaa
     2401 tgccaggctg cctgccagag tgagccctga ttccttttgt tggatcacca tcgtctttct
     2461 cctttactat gaccgtctga atgttatctt cagagtggtc tgtgcagatt tgagacaatg
     2521 gaatgtggag cttacattat gtcaagtatt ttttacaata tcgggcttga gttgaatacg
     2581 cgtgattgca atcatctctg gaaagctata ccgcggaaat ggttgagatg tcagcaccat
     2641 aacgcctttt gccatattct tggcttttag gacgtggtta atcatttctg accggcacga
     2701 ggacgggctg gtcgatattc atgatcttgt tacggtagtc aatgtattcc tggacgaggt
     2761 ctctgatcaa ttccttcacc gttgtgatat gcttcaccag ataggcattg gcacccgcga
     2821 aggcaaaacc atccgccata acaccgtttc tggcattgtt gagcgctagg gcaatacaat
     2881 agggggccac atgataatca caggttttaa ggcatttcca ggggcagata ataggcattt
     2941 tgacgccggc ggagacgtct ctgaggaatt tattgttgat agcgcggccg ggtaagccca
     3001 ccgggctttt gatgatcacg atctcttctt cccggcaatc cacaaagagc tgtttgaaag
     3061 ctggatcggc atcgcactcc tgggtggcca caaatctggt gcccatttgc acgccgtccg
     3121 ctccgagctc catgatccgg tagatctgct ctccggtaaa aatgccaccc gccgctatca
     3181 ctggaatgga cttggagtat ttgttttcga tctcccgaac ggcctctttg acttggggga
     3241 agaccttttc gaggctgtag tcctcgtcaa agatctgctc ttctttgaac cccagatggc
     3301 caccggcttt gggtccttcc aggacgacta tgtccggaat gcggttaaaa tgttccgccc
     3361 agtggctgag aatgattttg gctgctctgc cggaactcac gatgacgccg atcttggtct
     3421 tggtattttg cagatactca agggtcatgg tgctgggaac cttgagtggg agtcccgcgc
     3481 ccaaaaatac gatatcggct tcttcttcaa aagcagcgtt gatcatctca tcaaaatcgg
     3541 agatagccag catgatgttt actccgatga tccccttggt tttttggcgt gccgcgcgga
     3601 tttcctgttt gagagctgcg gcgttggcat ccttgtagga ttttccaggc tggggatgca
     3661 gaatccccaa acccactgat gatattatcc ctatcccgcc ttcgtttgcg accgccgcgg
     3721 ccaggcgact gagggatatt cccaccccca tgccgccctg gatgatagga agtttggcga
     3781 tcaggtcgcc gatcttcagt tggggcattt ttataagcat cttgtacctc gattccaggg
     3841 accgcgtcct cactgatgag ggctgcggat tcctggtaat tcgctgcaga aatcacgaaa
     3901 taatcgggtt tgctcgcctg gcaacgcgtt aaagcttcag atggcaaagt aaagaagact
     3961 attcagaaaa atctggtaac gtctgaagac aagcaaaatc acccgtgcgt ttgcgtcaaa
     4021 tcttttttac ccaatcgata atgagcctgg gcagcgtgaa gttatgcttg cctaaaccat
     4081 cccaagcagc ctgtgtcctg gcaggggtta aaatacttga gttttctgga tgggaggttt
     4141 tatgtgggca agttcttctc tctgtataag gtgtcactga aaacttcaaa ttggctcact
     4201 gacagtttct aatgctcaag cctgatggtt ttcaagcccc aagcaccaag tggtcctgac
     4261 atgctatctc cctcttttca gattattacg tgcataatcc tggtattagt tcaatacaaa
     4321 gaaataagtt ctttatgcgg caacgtatag gaatgagcaa aacaatattc catcacgaac
     4381 ctgccagatg ggagccatta tatgacaaat tgctgtcaat tcctatgaga tcgtgattcc
     4441 ggtgacttga caaaaggatg tcacaaactg agggcatttc cgctggtttg tcaagtgggt
     4501 gtcacgattt gacatttcag gtgtcacttc ttattagtta tctaattgtc ggagaataag
     4561 atgctaagtg cacgtttttt atcgtatctg cgcttgtcaa catccgtgaa aaaaggcttg
     4621 acacatagtg taaattcaat ttacagtc
//
LOCUS       DAOSFN010000166         4626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_16638_length_4626_cov_8.327499, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000166 DAOSFN010000000
VERSION     DAOSFN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16638_length_4626_cov_8.327499"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="PLO35_08850"
     CDS             complement(<1..1628)
                     /locus_tag="PLO35_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH79817.1"
                     /translation="MDYTFTIRFGRQVTPDYQTALGHAGKFRKFKPAKEGEAFNVVET
                     NNLEALEKFDSFTGLIQTIMDWNNARVLHEALELKPPEFQLPEKHEIAMCYRKHQADP
                     HPEEYCDAKSPDCWGCRLLEGIVLRHDKQNYDKNLRYWYQFGSFTDKHTWKINRELLT
                     RALEELVRIKSLEFCPVFQNVFYRRYIAALPLMIHMKDTQNWALIYKEDLSNSSKHWE
                     AVNVRHISISEAASLEAERAGMLARDGLAGKNADASDASNIRYVPATTFADIGGVDEI
                     IQSIRIMIELPIVKPRLFEHLGVKPYRGILLWGDPGNGKTLIAKAIAHEVSAHFIPVA
                     GPDILNKNFGQSEKNLRDIFEEAREHQPSIIFIDEIDSIAQTRLTGESAKWYATVVNQ
                     LMALMDGIRDYGNVTVLASTNRPDLLDAALLRPGRFDYKLEIKKPNLPGCKRVLEIAT
                     RGMPLAEDVDLFSFSESVIGYSAAEITFLAKEAAMVALRRAVEAQSISLDAVQDPDYS
                     FIRVTMSDFINALHLLKWQRRYVNLTYSLKDKKLT"
     gene            complement(1796..2434)
                     /locus_tag="PLO35_08855"
     CDS             complement(1796..2434)
                     /locus_tag="PLO35_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79818.1"
                     /translation="MKIEFIDLVSEAGLDGLNLSFGLDALTVLHDPYEVLSGPILSAL
                     AGQSEILSGSLLIDGVPLGDSIDGKALHRVFGYVFDEGIMLANLSLRENLLLPLRWLD
                     PRLSDADFEGQLKPWLELFDLRVDLKLRPAMVKPAVRKYLSYVRSLLLKPDYLLIDDP
                     YYLLNKYERGVMYQALSRLRSTHRMLIASSDDDFTGSFSSNCLELADLYPTD"
     gene            complement(2431..3195)
                     /locus_tag="PLO35_08860"
     CDS             complement(2431..3195)
                     /locus_tag="PLO35_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH79819.1"
                     /translation="MTPQAQKKSSADFFCFVLDTFTQLLRPLGKHRLSWTIITRQVLF
                     TGYEALPLISLIALGVGGLIILQGYNLLSTFGQGIWLHRILVTVVINELSGLITALVV
                     IARSGTAISTELGNMKVNREIALLKSFGISPNSYLVVSRVWGVVASMLVLAVYFNIIA
                     VVGGWLFTRLFSPLGFRSFIYDFIGVLSISNLLMALSKSLVFGLIISITACYQGLSVS
                     RASTEVPQRTIKAVVNSISAVILADMLITWLFWVLS"
     gene            complement(3204..3560)
                     /locus_tag="PLO35_08865"
     CDS             complement(3204..3560)
                     /locus_tag="PLO35_08865"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79820.1"
                     /translation="MKQTMFKIVAALFLALMIGGLAAQHNNVIGDVNIYPNPMDKQCE
                     ITIQVNQNAALGINIEDFQGNVIKSLYWGTTNKDVRLTWDRYSDNGEYVPSGTYFVVV
                     NYSGRYTSTKKTLILK"
     gene            complement(3609..4541)
                     /locus_tag="PLO35_08870"
     CDS             complement(3609..4541)
                     /locus_tag="PLO35_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH79821.1"
                     /translation="MNDRTPLYLGFDIGGSSVKWGYGNCQQGVLRFESTAITSKDLPC
                     LMSVFTQILFRVDSDIGLSSIRGIGIGTPGTIDRLSGKIHGTNPNLPFWTDLCPAMLI
                     PSETAIPVVYDNDANLMALGEATLREGISDLIGITVGSGIGSGFVWGGRLFHGAHGYG
                     MEFGHVTVVRDGEVCNCGRPGCLEAYASVDGIKRRASRLKEYPEAGSWDLRDLLKLAQ
                     QDERLRELIQEGEHYLALALTDLTVLLDPQLIVLGGGGIDGKLYSPQKIEDLIRARLP
                     RVNQPFIRVETAKAGNQAGVLGAIAYAASELSGT"
BASE COUNT         1006 a         1223 c         1333 g         1064 t
ORIGIN      
        1 gtcagcttct tatccttcag tgaataggtg aggttcacat agcggcgctg ccatttgagc
       61 aggtgcaggg cgttgatgaa atccgacatg gtgaccctga tgaacgagta gtccgggtcc
      121 tggaccgcgt ccagagagat gctttgggct tcgacggccc tgcggagggc taccatggcg
      181 gcttctttgg ccaggaacgt gatttccgcc gcggagtatc cgatcaccga ttcggagaag
      241 ctgaagaggt ccacatcttc ggccaggggc attccacgcg tggcgatttc cagcacccgt
      301 ttacagcccg gcaggttggg ctttttgatc tcgagcttat aatcgaagcg acccggtctc
      361 aagagcgccg cgtccaggag gtcgggacgg ttggtggaag ccagcacggt gacgttgccg
      421 tagtctctga tgccatccat cagggccatc aattggttga ccacggtggc ataccatttg
      481 gcagattctc cggtgaggcg ggtctgagcg atggaatcga tctcatcgat gaaaatgatg
      541 gagggctggt gctctctggc ctcctcaaag atgtcgcgca ggtttttttc ggactggcca
      601 aagtttttgt tgaggatatc gggacccgcc acagggatga aatgagcact cacctcgtgg
      661 gctatagcct tggcgatcag ggttttaccg tttccgggat cgccccagag caggatgccg
      721 cgatagggtt tcacgcctaa atgctcaaag agcctgggtt tgacgatcgg cagctcgatc
      781 atgatccgga tgctctgaat gatctcgtcc acgcctccga tatcggcaaa agtggtggcg
      841 gggacgtatc tgatgttgct ggcatccgag gcgtcggcat tcttgccggc cagaccatct
      901 ctggccagca ttccagcccg ctcggcttcc agactggcgg cttcggaaat gcttatgtgg
      961 cgtacgttta ccgcttccca gtgtttggag ctgttgctga ggtcttcctt atagatgagg
     1021 gcccagtttt gggtgtcttt catgtggatc atcagtggca gggcggcgat gtatctgcgg
     1081 tagaagacat tctggaagac ggggcaaaac tccagactct tgatcctgac gagctcttcc
     1141 agggcccggg tgagcaactc gcggttgatc ttccaggtgt gtttatctgt aaagctgcca
     1201 aactgatacc aatatctgag gtttttatcg tagttttgtt tgtcatgacg cagcacgatg
     1261 ccttccagga gacggcagcc ccagcaatca gggcttttgg cgtcgcaata ctcttccgga
     1321 tgcggatcgg cctggtgttt gcgataacac atggcgatct cgtgtttctc aggaagctgg
     1381 aattcgggcg gcttcagctc cagggcctcg tgcaggacgc gcgcgttgtt ccaatccatg
     1441 atagtttgaa tcagccccgt gaagctgtca aacttctcca gggcctccag attgttggtc
     1501 tccaccacgt tgaaggcctc gccttctttg gcgggcttga attttcgaaa tttacccgca
     1561 tggcccagag cggtctgata gtctggcgtg acctgtctgc caaagcggat cgtgaaagtg
     1621 taatccattg tttaccttcc ctggtttgat gggttccttt tggcgcgcgg agggattgtg
     1681 tcaatgaata tctgcgcccc gtcccgctct tctcactatc gcacgcttat cccgtgctta
     1741 tcgcacgcct aacgcgtgct ttggcatggg ataggagtga gataagccag cgttatcagt
     1801 cggtgggata caagtccgca agctccaggc agttagagct gaaagagccg gtaaaatcgt
     1861 cgtccgaact ggcgatcagc atcctgtgag tggagcgcaa gcggctgagg gcctgataca
     1921 tcaccccgcg ctcgtatttg ttgagcaggt aatagggatc gtcgatgagc agatagtccg
     1981 gcttgaggag cagcgagcgg acatagctaa ggtacttgcg gacagcgggt ttgaccatgg
     2041 ccggccggag cttgagatcg accctgagat caaagagctc cagccagggt ttcagttgcc
     2101 cctcgaaatc cgcatcgctg agcctgggat cgagccagcg gagcggcaag aggagatttt
     2161 ctctgaggct gagattggcc agcatgatcc cctcgtcaaa gacgtatccg aaaacccggt
     2221 gcagggcttt tccatctatg ctgtcgccaa gtggaacgcc atcgatcagc aggctgccgc
     2281 tcaggatttc actctgaccg gccagagcgc tcagtatcgg tccgctcaat acttcatagg
     2341 gatcgtgcag cacggtgagg gcatccagcc cgaagctgag attcaagccg tccagaccgg
     2401 cctcgctgac cagatcgata aactctatct tcatgacagc acccaaaaca gccaggtgat
     2461 cagcatgtcc gccaaaatca ccgccgaaat gctgttcacc accgctttaa tagtgcgctg
     2521 gggaacttcg gtgctggcgc ggctcacgct aagcccctga taacatgcag ttatggagat
     2581 aatgagacca aataccaagg acttgctgag ggccatcagg aggttggaaa tgctcagcac
     2641 gccgataaaa tcatagataa aagagcgaaa ccccaggggg gaaaagagcc tggtaaacag
     2701 ccagccgccc acgacggcaa tgatgttgaa atagacagcc agcaccagca tcgatgccac
     2761 caccccccaa accctcgaga ccaccagata ggaattgggt gatatcccaa acgacttgag
     2821 gagagctatc tccctgttta ccttcatgtt gcccagttcg gtggagatgg cggtgccgct
     2881 gcgggcgatc actaccagag cggtgatcag tccactcagt tcgttgatca ccaccgtcac
     2941 gaggatgcgg tgcagccaga tgccctgtcc aaaggtgctg aggaggttgt agccctgcag
     3001 gatgatcagg ccgcccactc ccagagcgat cagactgatg agcggcaggg cctcgtaacc
     3061 ggtaaaaagc acctggcggg tgatgattgt ccagctgagg cggtgttttc ccagcggcct
     3121 cagaagctgg gtgaaagtat ccagaacaaa gcaaaaaaag tccgcggagg actttttttg
     3181 ggcttgaggg gtcatgctaa agtttacttc agaatcaggg tcttcttggt ggatgtatag
     3241 cggccggagt agtttaccac cacgaaatag gtgccgctgg gcacgtattc gccattgtcg
     3301 gaatagcggt cccaagtgag gcggacgtct ttattggtag tgccccaata gaggcttttg
     3361 atcacatttc cctgaaaatc ttcgatgttg atcccgaggg cggcgttctg gttgacctgg
     3421 atggtgattt cacattgctt gtccatgggg ttgggataga tgtttacatc accgatcacg
     3481 ttattatgct gggcagccag gcccccaatc atcagcgcca aaaagagcgc ggccactatc
     3541 ttaaacatag tctgtttcat gataaactcc tgaaatttgt tagagaactg aggtagcaag
     3601 tttcgttcct atgtgcctga tagttcgctg gcggcatagg caatcgcgcc caaaactcca
     3661 gcctggttgc cggctttggc ggtttccact ctgataaagg gttgattcac cctcggcagg
     3721 cgcgctctga tcaagtcctc tatcttttgg ggggagtaga gtttgccatc gatgccgcca
     3781 cctcccaata caatcaattg ggggtccagc aacaccgtta aatccgtcag ggccagggcc
     3841 agataatgct ctccctcttg tatcagttcc cgcaggcgct catcctgttg cgcgagcttc
     3901 agcaagtctc tgaggtccca gctgccagcc tcgggatact ccttcagacg actggcgcgg
     3961 cgcttgatcc cgtccacgga ggcgtaggcc tccaggcagc cagggcgtcc gcagttgcag
     4021 acctcgccgt cccggaccac agtaacgtgg ccaaactcca tgccataacc atgggccccg
     4081 tgaaagagtc ttcctcccca gacgaatccg ctgccgatcc cgctgccgac agtgatgccg
     4141 atcaagtcgc taatcccttc tctgagagtg gcttcaccca gggccatgag attggcatcg
     4201 ttgtcgtaaa ccactggaat ggctgtttct gaggggatga gcatggcggg gcagaggtct
     4261 gtccagaaag gcagattggg attggtgccg tggattttgc cactgaggcg gtcgatggtg
     4321 ccgggggtgc cgattccgat cccgcggatg gaactcaggc caatatcgct gtcaacccta
     4381 aagaggattt gcgtgaagac ggacatcagg caagggaggt ctttgctagt aatagcggtg
     4441 ctttcaaagc gcagtacacc ttgttgacag ttgccatagc cccatttgac agaggagccg
     4501 ccaatgtcaa aacctagata gagaggggtt ctatcgttca ttttcataat caggtggggt
     4561 gggtgatggg actcgaaccc acaacgcccg gaaccacaat ccggtgctct gccattgaac
     4621 tacacc
//
LOCUS       DAOSFN010000167         4613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_16701_length_4613_cov_10.641509, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000167 DAOSFN010000000
VERSION     DAOSFN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16701_length_4613_cov_10.641509"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLO35_08875"
     CDS             <1..492
                     /locus_tag="PLO35_08875"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH79822.1"
                     /translation="ASRQRSNPAIQSLELAIREETIPIMDDIQIQVVKQADSAQVLEL
                     YRLAGWWQTDDNPSYLDMVQGIVEKTHCFVTATLGDRIIGMGRAISDGVSDAYIQDVT
                     VHPELRGQGIGKAIIRKLVERLRADGLQWIGLISEPGYQGFYSSLGFAEMSGYTPFLL
                     IQD"
     gene            500..1387
                     /locus_tag="PLO35_08880"
     CDS             500..1387
                     /locus_tag="PLO35_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOH79823.1"
                     /translation="MDIKLHELTLLHVPMLIDWLKKYPREACDYSVCNLMTWGKIYHN
                     QFCEYAGRLVFYNPQYAYIFFPVGEWMAPQELETLVNDFRKLNPEASLILIPEKYTLA
                     YPTLCHHFELHDDRAWADYVYATERMVKLSGKKLAKKKNLISQFTRAYPDYKVLQITP
                     SRREVIIQFTQKWKREREVEGIYLNTEFKAIQNTMEMWDSLPVDGIIICLHNRIVAYS
                     IFSPQTADMATIHFEKFDPDKKGSAQVINWETAKYLQDDYAWINREQDIGLEGLRQAK
                     SSYMPDRMVKFISGKLKQP"
     gene            1612..4518
                     /locus_tag="PLO35_08885"
     CDS             1612..4518
                     /locus_tag="PLO35_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79824.1"
                     /translation="MKPIIFAVLLLTLFASFVGAEQVWPQPLELYSAQNIEFGGKGLK
                     TSSGNYLVSWKDTREPQLKQQFLLTNPDGDSLWQRSIPLRISGEDAMLRWMIETGGDY
                     LLLFSRGNGFLHGFRLSASGEHVWPETGIQLLHSNLINPEIFSKLISDGNGGYIHLWA
                     WTFSGINNPAAYYFQHMDAQGETAIINGPVSNEHILVSDPVLSGPPDLFMLADGGLIC
                     AYRENDNARVRRMGPNHLFIYTHDIPIGAPVWPHLVDTGDGCFNLIWNTEDAIMALRY
                     NYDFSLQWPMALDLCQATSGLTASNCISVRGPDSKTLTVWKSGNQLKMQRFTTAGVLE
                     CGSYGTTIMDGVSESASFELKPDQSGGCFIIVTDQTNHIASAQYITPQNQVLPQPVLL
                     ALSLPGVHVGPGFDSAALDDGLHFYHQISAPGFGAIRQTRVDSSGAIIQPGDLNDLVS
                     GHSGKALSPLAVAAGSRIFTAWMDEPGYPVSYAGSRQIHYQWVNRDGSVVLNEPATIF
                     EANDLRPVLEIKAIKTDTENVLICWWEGYSPPRLKAQLIDSSGNQLWEPGGRLIADEG
                     LQSLGAYGYIGLTQAHGAVFILWMKQHLGPAGLTIHLQKVVANQIAWEYDGRWFTNIE
                     HQNNQLIGVEQNYLFTGPAIPGQDIYQWADVWSFDAEGGFLNSHYIGSNIAIRSFKLG
                     QDRLYVTYGLRVTIPESYYYLSYGQAFSLTLEPLWGPDGIDNFDGNCLLTDNNDLYLA
                     RGYQQATLHGFDPSGQEFCGFNVPSDPPGAQYLQVLQLQTSWEDRFVGLARSSASYGG
                     TRLYYFSFDATGWNALDSDFLLAEGAPLRTAELCRLYDDAYALWGTSATFSDTDLRNL
                     RIQRINSHLVDNDDPIAPELPKPTLSHAYPNPFAGSVSFTVTLPQRTRAEITIYNLRG
                     QLTRHLHSGSLEKGKSSFEWDGRDERGASVANGIYLCKARLGSQSRTIRLVKLR"
BASE COUNT         1050 a         1424 c         1189 g          950 t
ORIGIN      
        1 gcctcccgcc aaagaagtaa ccctgccatc cagagcctgg aattggcaat cagagaggag
       61 actatcccta tcatggacga catccaaatc caagtggtca aacaagcgga cagcgcgcaa
      121 gtgctggaac tctaccggct ggcaggctgg tggcagacgg acgacaatcc cagctatctg
      181 gatatggtgc aaggcattgt ggaaaagacc cactgctttg tgaccgccac tctgggagac
      241 cggatcattg gaatgggccg ggctatcagc gacggcgtca gcgatgccta tattcaggat
      301 gtgacggtgc atccggagct gcgcggccag ggcatcggca aggccatcat ccgcaagctg
      361 gtggagcggc tccgggcgga tggcctgcag tggatcggcc tgatctcaga gcccggctat
      421 cagggctttt acagctccct cggctttgcc gaaatgagcg gctacacccc ctttctgctg
      481 attcaggact gaggcccgct tggatatcaa acttcacgag ctcaccctcc tgcacgtgcc
      541 catgctgatt gactggctca agaaatatcc ccgcgaagcc tgtgactaca gcgtctgcaa
      601 cctcatgacc tggggcaaga tctaccacaa ccagttctgc gaatacgccg gccgcctcgt
      661 gttttataat ccccaatacg cctatatctt ctttcccgtg ggagaatgga tggcccctca
      721 ggagctggaa acgctggtga atgactttcg aaagctcaat ccggaggcct cgctgatcct
      781 gatcccggaa aaatacaccc tggcctatcc cactctctgc catcactttg agctgcacga
      841 cgaccgcgcc tgggccgact acgtctatgc cacggaacgg atggtgaagc tctccggcaa
      901 aaaactcgcc aagaaaaaga acctcatcag ccagttcacc cgcgcctatc cggattacaa
      961 agtactgcaa atcacccctt cccgccgtga ggtcatcatc cagttcaccc agaaatggaa
     1021 acgcgaacgc gaggtggaag gcatctacct caacaccgag ttcaaagcca tccaaaacac
     1081 catggaaatg tgggattcct tgcccgtcga tggcattatc atctgcctcc acaaccgcat
     1141 cgtggcctat tccatcttca gtccccagac tgccgacatg gccaccattc actttgagaa
     1201 atttgacccc gacaagaaag gcagcgctca ggtaatcaac tgggaaacag ccaaatacct
     1261 ccaggatgac tatgcctgga tcaaccgcga acaggatatc ggcctggaag ggctgcgtca
     1321 ggccaaaagc agctatatgc cagaccggat ggtcaaattc atcagcggca agctcaagca
     1381 accctgagcc ggaaacgcca gggctttgct ggcacccggt cccaataaac cccgtgcatc
     1441 caggctccca gcccggcagc gcttaaggac ttccgcggcg cgggccgcac cttctcacct
     1501 gcacccgcaa tagatttccc tgcctcatca ccataagatc tgaaaaaatt gcttgactcc
     1561 ccgcgggatt caaatatcct gctctctact cacttccagc agggaggaat tgtgaaacca
     1621 atcatttttg cggtattgct gctaaccttg tttgccagct ttgtgggggc cgaacaggtc
     1681 tggccccagc ctctggagct ctactccgcc cagaatatcg agtttggcgg caaaggcctg
     1741 aagaccagct ccggcaacta tctggtctcc tggaaagaca cccgggaacc gcagcttaag
     1801 cagcaattcc tcctcaccaa tccggacgga gacagcctct ggcagcgttc catccctctg
     1861 aggatcagtg gcgaggacgc catgctgcgc tggatgatcg aaaccggggg ggactatctc
     1921 ctgctgtttt cacgaggaaa cggctttctg cacggcttcc ggctcagtgc ttccggtgag
     1981 catgtctggc ctgaaacggg gatccagctc cttcactcca acctcatcaa tcccgaaatc
     2041 ttctccaaac tgatctcaga cggcaatggc ggctatatac atctctgggc ctggactttc
     2101 agcgggatca acaaccccgc cgcctactat tttcaacaca tggatgccca gggcgaaact
     2161 gccatcatca acggccccgt cagtaatgag catatcctgg tgagcgatcc cgtcctgagc
     2221 ggcccacccg atctctttat gctcgcggac ggtggattga tctgcgccta ccgtgaaaac
     2281 gacaacgcca gggtgcgcag aatgggcccc aaccatctct tcatctacac tcacgacatc
     2341 cccatcggag cgccggtctg gcctcatctg gtggacaccg gtgacggctg tttcaacctc
     2401 atctggaaca ccgaggacgc gattatggcc ctgcgctaca actacgactt cagtctgcag
     2461 tggcctatgg cactggacct ttgtcaggcc accagcggat tgacggcctc caactgcatc
     2521 agcgtgcgtg gcccggactc taaaaccctc accgtctgga agagtggcaa ccagcttaaa
     2581 atgcaacgct ttacaacagc aggcgtcctg gagtgcggca gctatgggac caccatcatg
     2641 gacggagtgt ctgagtcagc ctcgtttgag ctcaagccag atcagtctgg cggatgtttt
     2701 attatcgtga ctgatcaaac caaccatatc gcctcggccc agtacatcac tccccaaaac
     2761 caggtgctgc cacagccggt gctgctggcc ttatcccttc cgggagtgca tgttggccct
     2821 ggttttgaca gcgccgccct ggatgacggc ctgcactttt accaccagat tagcgctccc
     2881 ggttttggcg ctatccgtca aacgcgggtt gactcgtccg gggccatcat ccagcccggc
     2941 gatctcaatg acctcgtatc aggccactct ggcaaggcct tgtccccttt ggcagtagca
     3001 gccggcagca ggattttcac cgcctggatg gatgagccgg gctatccagt ttcctacgcg
     3061 ggctcgcgtc agatccacta tcaatgggtg aaccgggacg gcagcgtggt gctgaatgag
     3121 cctgctacca tctttgaggc caacgattta aggccggtgc tggagatcaa ggccatcaag
     3181 acggatacgg aaaacgtgct gatctgttgg tgggagggat attccccgcc cagattaaaa
     3241 gcccagctga tcgactcctc cggaaaccag ctctgggaac cagggggcag gctgatcgcc
     3301 gatgaaggct tgcaaagcct tggagcgtat ggctatatcg gactcaccca agctcatggg
     3361 gcggtgttta tcctctggat gaaacaacat cttggcccgg ctggcctcac catccacctt
     3421 cagaaagtcg ttgccaacca gatcgcctgg gaatatgatg gcaggtggtt tacgaacata
     3481 gaacaccaaa acaatcagct gatcggcgtg gagcagaact acctctttac cggccccgcc
     3541 atccctggcc aggacatcta ccaatgggcc gatgtgtgga gctttgacgc ggaaggcgga
     3601 tttctgaaca gtcactatat cggctcgaac atcgctatca ggagcttcaa gctgggccag
     3661 gacaggctct atgtgaccta cggactgcgc gtcacaatcc cggaaagtta ctattactta
     3721 agctatggcc aggccttttc cctcacgctc gaaccgcttt ggggacctga cgggatcgac
     3781 aattttgacg gcaattgcct cctgaccgat aacaacgacc tctatctggc ccggggctat
     3841 cagcaggcca ccctgcacgg atttgacccc tccggccagg aattctgcgg cttcaacgtt
     3901 cccagcgatc ctcccggagc ccagtatctg caagttctcc agctccagac ctcctgggaa
     3961 gaccgcttcg tgggcctggc ccgcagctca gcctcctacg gaggcaccag gctctattac
     4021 ttcagctttg acgccaccgg ctggaacgct ctggacagcg atttcctgct ggcggaagga
     4081 gctccgctca ggacagcgga gctctgtcgg ctctatgacg acgcgtacgc gctctggggc
     4141 acttcggcaa cctttagtga tactgatctt cgcaatctca gaatccagcg aatcaactcc
     4201 catctggtgg ataacgacga tcccatagcc ccggaactgc ccaagcccac tctcagtcac
     4261 gcttatccca atcccttcgc gggttccgtg agctttaccg tcaccctgcc ccaaagaacg
     4321 cgcgcggaaa tcactatcta caacctccgt ggtcagttaa cccgccacct ccattccgga
     4381 agcctggaaa agggtaaaag cagctttgaa tgggatggac gggatgagcg cggtgccagc
     4441 gtggccaacg gcatctatct ctgcaaagcc aggctgggca gccaatccag gaccatcaga
     4501 ctggtcaaac tgcgctgaac ttactcgtaa cactgacttc agagcttgtg cgaaaatggc
     4561 gcattgagtc ccatcgggac ggcagtttag ctagatctgc gagccttctg cca
//
LOCUS       DAOSFN010000168         4588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_16854_length_4588_cov_10.866755, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000168 DAOSFN010000000
VERSION     DAOSFN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16854_length_4588_cov_10.866755"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2331
                     /locus_tag="PLO35_08890"
     CDS             <1..2331
                     /locus_tag="PLO35_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH79825.1"
                     /translation="DANSKANAAVVQAFRQKYPHIELRAFSGIKIENMDLDAGPLMAI
                     AGGVAPDILYVNFRQSDTYIQNNFLYPLDEFISRERPEAMQLRVEKPVWQVIKRKLKG
                     SSEEKVWMLPYETLVRVLMYRKDVFRRVGLDPNQPPRDWEQFKAYAARLSDPATNTYG
                     TVLAAGPQAAYDWLPLLWAAGGDAVLWDPVQEVWRASFASEAGVRAIEYYLELLNQPW
                     KDKNGRPQRGFAMREGDWGYLWTEGKIAMRNDYLSQQNMGGKYDPNLYGFAPSPAGPS
                     GQGGSEINCRMMGIFSGAGISNDAGLGDRDPKLVREAAWEYIRFYDSEEARQIRLKVM
                     VESGYGRTQNPVFLKRYGYDDYLRYAPEGWLETFETAMREGKPEPFGDNCQKVYEFMT
                     YPLDELIKLDRSGRLGNDPQTRRAAILRVLQKAQERTNAEMIGTLPKAEKIRRERLAA
                     VIAALILFAFLLTLWRVWTILAPQTKNSASPSSKRWFWIALLLAPAVISIFMWKYVPL
                     ISGSLMAAKDYKLVGHSPWVGFGNLAEVLFDPSWWASVGKTLYYMALSLGFGFLPPII
                     LAIMLQEVSHAKLLYRVIFYLPAVISGVIVIYLWKLLYAPGDAGALNQLLLALGLPKS
                     DWIRNESLAMLCVVLPGIWAGMGPGSLIYLAALKNIPNELYEAADIDGAGFLAKLRHI
                     VFPSLRALIIIQLIAAFIIAAQSSDFILVMTFGGPNEATRVADLLIFEKAYLYLKFGL
                     ATTMAWMLSLMLMGFTVMQIKHISRMEFRVANQEER"
     gene            2378..4246
                     /locus_tag="PLO35_08895"
     CDS             2378..4246
                     /locus_tag="PLO35_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH79826.1"
                     /translation="MSIIGKVARRDPKTRALNLAMHLILILGSLTMLYPFALMLSSSI
                     KSAVDGTRMELIPPYLYQDAALYQKYLESRYNEESSRLMDNYPGSWISFAEVKLPDNP
                     DPAVWQDFKAFLKQSDYSVYHYYVAEHYGRGVYPLAQRQYRKILRSENSNSLVEFNKR
                     YGTGAVSWEEIVVEEKEITRRLFTSSSSGYLGRFRKFKEQTPLWQRLFVDPDGCFVNS
                     ELIPSAGGDLAKFNGLNGTSYQAWTDIRLPSECPPEGHHLREPWLRYARAVLNIHQLG
                     LTDSALPAFRAMLKDKYGSIGLLNLTWKLDLSSFEQVTIPQQLPDSGALVEDLSFFIQ
                     NQAQPEQIRVHSLAHNWRDWLRRKYGSLASLNLARRDSLPSWDAIAFPVREMDYFSFL
                     ERKAAIRWEFISRNYKMALDQMLSDARSLRNTGIYVLLSILLAVTVNPLAAYALSRFK
                     PRFSYQLIMLFMLTMAFPAMVMGIPNFLMLKRLNLLNTFWALVLPAAADGYFIFLLKG
                     FFDSLPREIYESASLDGASEFRLFWQFTLWLSKPILAVIALGAFNAAYRNFLFAFIVC
                     QDQSMWTLMVHIYNLMQRASVSVGYAALVIAAIPTLAVFIFFQNIIIKGIVVPMEK"
     gene            4274..4552
                     /locus_tag="PLO35_08900"
     CDS             4274..4552
                     /locus_tag="PLO35_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79827.1"
                     /translation="MFKLILVRFDGDAGRFEDEEFNKFCAEQNIVRIEKEFINTGGDI
                     YYSFFIEYHSRKAGYEKPDIAKLSERELEQYNRLRDWRNELAVFIMTA"
BASE COUNT          987 a         1356 c         1235 g         1010 t
ORIGIN      
        1 gatgccaatt ccaaggccaa cgccgcggtc gtgcaggctt ttcgccagaa gtatcctcat
       61 atcgagttga gggccttttc cggcatcaag atcgaaaaca tggacctcga tgccggtcca
      121 ctgatggcca tagccggggg agtggcaccc gacatcctct atgtaaactt ccgccagagc
      181 gacacttata tccaaaacaa ctttctctat cccctggatg aattcatctc ccgcgagcgc
      241 cctgaggcca tgcaattaag ggtggaaaag cccgtctggc aggtgatcaa gcgcaaactg
      301 aaaggctcat cggaagaaaa agtctggatg ttgccctacg agaccctggt gcgggtgctg
      361 atgtatcgca aggacgtctt tcgcagggtg ggcctggacc ccaaccaacc tccccgggac
      421 tgggagcagt tcaaagccta cgccgccagg ctaagtgacc ccgccaccaa cacatacggc
      481 accgtcctgg ctgccggacc gcaggccgcg tatgactggc tgccgcttct atgggccgcg
      541 ggtggagacg ctgtgctctg ggaccccgtc caggaagtct ggcgcgccag ctttgcctcc
      601 gaagcgggtg tgcgggccat cgagtattat ctggagctgc tcaatcagcc ctggaaggat
      661 aagaacggcc gtcctcaacg cggttttgcc atgcgtgagg gcgattgggg ctatctctgg
      721 acggaaggca agatcgccat gcgcaacgac tatctttccc agcaaaacat gggcggcaaa
      781 tacgatccca acctctacgg cttcgctccc agtccggcag gcccatccgg ccaaggcggc
      841 agcgagatca attgccgcat gatgggtatc ttcagcgggg ccgggatcag caacgacgcc
      901 ggcctgggag accgcgatcc caagcttgtc agagaggcag cctgggaata tatccgcttt
      961 tacgacagcg aggaagcgcg tcagatccgc ctcaaggtga tggtggaaag cggttatggc
     1021 cgcacccaga atcccgtgtt tctaaagcgc tatggttatg acgattacct gcgctatgcc
     1081 cctgagggct ggctggaaac cttcgagacc gctatgcggg aaggaaaacc cgagcccttt
     1141 ggcgataact gccagaaagt atatgagttt atgacttatc cgctggatga gctgatcaag
     1201 ctggaccgct ccggtcgcct gggaaatgat ccccaaacgc gcagggcggc cattctcagg
     1261 gtgctccaaa aagctcagga acgcaccaat gccgaaatga tcggcactct ccccaaggcc
     1321 gaaaagatca ggcgggaaag gctggcagcc gtgatcgcgg ccctcatcct cttcgctttc
     1381 ctgctcactc tctggagggt gtggaccatc ctggctccgc agactaaaaa cagcgcttcc
     1441 cccagctcca aacgctggtt ttggatagcc ttattgctgg ccccggcggt gatcagcatc
     1501 ttcatgtgga aatacgtgcc gctgatctcg ggatcgctga tggcggccaa ggactacaag
     1561 ctggtgggcc acagtccctg ggtgggcttt ggcaatctgg cggaagtgct cttcgatccc
     1621 agctggtggg cctcggttgg caagactttg tattacatgg ccttatcact aggctttggt
     1681 tttttgccac ccatcatcct ggccatcatg ctccaggaag tttcccacgc caagttgctc
     1741 taccgggtga tcttttatct gcccgccgtg atcagtggag tgatcgtaat ctatttgtgg
     1801 aagctgcttt acgccccagg cgatgccgga gcgctgaatc aattgctgct cgcgctgggc
     1861 ttgcctaaaa gcgattggat cagaaacgag agcctcgcca tgctctgtgt ggtgctgccg
     1921 ggtatctggg ccggcatggg acccgggtcg ctgatctatc tggcagcgct aaagaacatc
     1981 cccaatgagc tttacgaggc cgcggacatc gacggggcgg gttttttggc caagctcagg
     2041 catatcgtct tccccagcct cagagcgctg atcatcatcc aactgatcgc cgcctttatc
     2101 atcgccgccc aatccagcga tttcatcctc gtgatgacct tcggaggccc caatgaagcc
     2161 acccgcgtgg ccgacctcct gatctttgaa aaggcctacc tctatctcaa attcggcctc
     2221 gccaccacca tggcctggat gctgagcctg atgctgatgg gcttcaccgt gatgcagatc
     2281 aaacacatct cccgcatgga gttccgcgtg gccaaccagg aggaaagatg agccagatgg
     2341 cttcgcggat gtcccattct gcctttacga ggccctcatg agcatcatcg gcaaagtcgc
     2401 ccgccgcgat cccaaaaccc gggccctcaa cctcgccatg caccttatcc tcatcctggg
     2461 ctcgctcacc atgctctatc cctttgccct gatgctttct tcctcgatca aaagtgctgt
     2521 agacggcacc cgcatggagc tcatcccccc ctatctctac caggacgcgg cgctctatca
     2581 gaaatatctg gaaagccgct acaacgagga aagcagccgc ctcatggaca actatcccgg
     2641 ctcctggatt tccttcgcgg aagtgaagct tcctgataat cccgatccgg cagtatggca
     2701 ggactttaag gccttcctga agcagtcgga ctacagcgtt tatcactatt acgtggccga
     2761 gcattatggc cgcggcgtct atcctctggc ccagcgccag tatcgcaaaa tccttagaag
     2821 cgaaaactct aactcgctgg tggagttcaa caagcgctat ggcaccgggg ccgtgagctg
     2881 ggaggagatc gtggtggagg aaaaggaaat cacgcggcgc ctcttcacca gctccagctc
     2941 gggatatctg ggacgcttcc gcaagttcaa agagcagact cccctctggc agaggctgtt
     3001 cgtggacccg gacggctgtt ttgtgaacag tgagctcatc ccctcagcgg gaggggacct
     3061 ggccaaattc aacgggctca acggcacatc ctatcaggct tggacggata ttcgcctgcc
     3121 gtcggaatgt cctccggagg gccatcatct gcgggaaccc tggctgcgct atgccagagc
     3181 ggtgctcaac atccatcagc tggggctcac agacagtgcc ctgccagcct tccgggccat
     3241 gctcaaagac aaatacggca gcatcggtct gctcaacctt acctggaagc tcgatctctc
     3301 ctctttcgag caagttacta ttccccaaca gcttccggat agcggagcgc tggtggaaga
     3361 cctcagtttc tttatccaga atcaggccca gccggagcag atcagggtcc acagcctggc
     3421 gcataactgg cgggactggc tgcgccgcaa atacggcagc ctcgcgtcgc tcaatctggc
     3481 cagacgggac tctttgccaa gctgggacgc tatcgctttc ccagtcaggg agatggacta
     3541 cttcagcttt ctggaaagga aggcggccat ccgctgggaa ttcatcagcc gaaactacaa
     3601 gatggccctg gaccagatgc tcagcgatgc caggtcgctt cgaaacaccg gcatctacgt
     3661 attgctctcc atcctcctgg ccgtgaccgt caaccccctg gcggcctatg ctctcagccg
     3721 tttcaaaccc cgcttcagct atcagctcat catgctcttt atgctcacga tggccttccc
     3781 cgccatggtg atgggcatcc ccaatttcct catgctcaag cgcttaaacc tgctcaacac
     3841 cttctgggcc ctggtgttac ccgctgccgc ggatggctat ttcatctttt tgctcaaagg
     3901 cttcttcgac agcctgccgc gcgaaatcta tgaaagcgcc tctctggacg gggccagcga
     3961 gtttcgcctc ttctggcagt tcaccctctg gctctccaaa ccgattttgg cagtgatcgc
     4021 gctgggagct ttcaacgcgg cataccgcaa ctttctcttc gccttcatcg tctgtcagga
     4081 ccagagcatg tggaccctca tggtgcacat ctacaacctc atgcaaagag caagcgtgag
     4141 tgtgggttac gccgctttgg tgatcgccgc cattcccact ctggccgtat ttatcttctt
     4201 tcaaaacatc atcatcaagg gaattgtggt gccgatggag aagtgagccc gccggttttt
     4261 ttaagggaag ataatgttca aactaatctt agtccgtttc gacggggatg ccggacgctt
     4321 tgaagatgag gagttcaata agttttgtgc agagcaaaac attgtgcgga tcgagaagga
     4381 attcatcaac acgggtggcg atatctacta ttctttcttt attgaatacc actctcggaa
     4441 agctggatat gaaaagccag acattgccaa actgagtgaa agggaactgg agcaatacaa
     4501 ccgactgcgg gactggcgca atgagttggc tgtctttatc atgaccgctt gaactccaac
     4561 ggggtaatgt cagtctctgg gttttaga
//
LOCUS       DAOSFN010000169         4537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_17131_length_4537_cov_9.357876, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000169 DAOSFN010000000
VERSION     DAOSFN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17131_length_4537_cov_9.357876"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..995
                     /locus_tag="PLO35_08905"
     CDS             144..995
                     /locus_tag="PLO35_08905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOH79828.1"
                     /translation="MPRNKLPNPMTKLIKKPAPSRIKNFRAHIPICLDKKNFIVKTAD
                     STEELRAALKLRHDVFLDELLKRKKKSGLDIDRFDKHCDHLLIIDKRNGMIIGTYRLQ
                     SSLHTKRWYTATEFHMKHILKRLPGNKLELGRACVHPDYRNGVTIALLWEGIGTYIAA
                     SQTSYLFGCSSIKTMDKEEIRGIYQYLKQNGHLTDDHRVRPRNKFKVPGMARHARRKH
                     QLSSLDELSAHKDKIPALLNSYLKAGAKVCGVPALDRSFKCIDFLTLLRVQDMGAGYG
                     RKYNKDH"
     gene            1000..1779
                     /locus_tag="PLO35_08910"
     CDS             1000..1779
                     /locus_tag="PLO35_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH79829.1"
                     /translation="MRLTKLPHLIVNTAVLATYFGVGFVLQWITRDPIKRRRRAIRFT
                     STMCRVFLKTLNIEIKVEGREKLQALHKSSYLMVSNHSSYTDILVLSSLENLVFITSV
                     EMGNNPVLGDITRLGGCLYTDRKKPVSLPKEIQRFSATISEGFKVVLFPEGTSTDARS
                     IKDFRASLFQVAIGARATILPVCISYQSVDGQPFGDNNRDTICWYGDMDFVPHFIGLL
                     NHQIGVRVTILDPIPFDPNLSRAELSDLTREALLKTYHIDR"
     gene            1792..1986
                     /locus_tag="PLO35_08915"
     CDS             1792..1986
                     /locus_tag="PLO35_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79830.1"
                     /translation="MAPKKDRLGLGKINFLLLALAAILLIAGYFIMSLNEITISPLIL
                     AGVYVLLIPFALLYRPKPKE"
     gene            1993..2871
                     /locus_tag="PLO35_08920"
     CDS             1993..2871
                     /locus_tag="PLO35_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOH79831.1"
                     /translation="MPILSDETAAKLKSCQLNAKAIVEGFLVGLHRSPYHGFSVEFSD
                     HREYNEGEPLKDVDWKIVARTDRYYVKRYEEETNLRCYILIDHSKSMFFQSGALTKIA
                     YATQLAAALAWLMIGQKDAAGLITFNDHVTGQYAPKAYRGYLPILFRALVELEPGGET
                     DVSSALHSIAETIRKRSLIVLISDLLDEPESIAQGLKHFRARHHEVLVFHIADLMEEN
                     FSFRRETEFVDSETGEKLTVTPWQIRQRYLESYGQHLEALKAQFRQFQIEYNPVSTAE
                     PLNDLLLKYLLKRRKG"
     gene            2933..3787
                     /locus_tag="PLO35_08925"
     CDS             2933..3787
                     /locus_tag="PLO35_08925"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOH79832.1"
                     /translation="MSVTRRIDAYDLFMAVKNGLNQIYCQPDPAVISKIAALAKKEKS
                     PLAKDMLTAITVNAEIAAREGIPCCQDTGTVVFFIELGERAVLVDGTIENTIKAVLRL
                     GWYRLNLRRSLVEEPLFERQIIPDTVPPVIHWEINPGNQIKVTMGLKGGGAENMSTLK
                     MMPATSTVEQIKQAVVETVVNAGGKACPPVLVGVGIGGNYESCAVMAKRALFEELGRA
                     HPDPRYATLEKEILAEINRQGRGVMGMGEGPSALAVHIKTAPCHIASLPLAVNLDCHA
                     HRHYSFTL"
     gene            3815..4318
                     /locus_tag="PLO35_08930"
     CDS             3815..4318
                     /locus_tag="PLO35_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOH79833.1"
                     /translation="MNIHKLRLPLATHSSSTLKAGDKVLLSGTLYTARDMAHKRLAAL
                     IAAGNPLPWDLSEGALFYCGPSPVPKGKICGAIGPTTSARMDPFTPLLLEHGLRVMLG
                     KGERSPEVQKAIRHHGAIYLAAVGGISALLAQHVIKFELFLWPELGAEAVYRMEVEDF
                     PCYVMTV"
BASE COUNT         1125 a         1330 c         1181 g          901 t
ORIGIN      
        1 aaaaaatagt atctcggttt acggaggggt ccagagcctg atacaaatta agaaatcttc
       61 acgccgcaac aaagcgcttg acaagccagc cccctccatc cgtgatggct tttagcaaaa
      121 aataccaagc aaggatagcc cagatgccgc ggaacaagct gcccaatccc atgaccaagc
      181 taatcaaaaa gccggctccc agccggatca agaacttccg cgcccacatc ccgatctgcc
      241 tggacaagaa gaacttcatc gtgaagacag cggacagcac ggaagagctg cgggccgccc
      301 taaagctgag gcacgacgtc tttctggatg aactgctcaa acgcaaaaag aaaagcggcc
      361 tcgatatcga ccgctttgac aaacactgcg accatctgct catcatcgac aaacgtaacg
      421 gcatgatcat cggcacctat cgcctccagt cctccctgca caccaaacgc tggtacacgg
      481 ccaccgagtt tcacatgaaa cacatcctca agcgtctgcc cggcaacaaa ctggagctgg
      541 gaagggcctg cgtgcatccg gactatcgca acggtgtcac gatcgccctg ctctgggaag
      601 ggatcggcac ctatatcgcc gccagccaga cctcctatct cttcggctgc tccagcatca
      661 agaccatgga caaagaagag atccgcggca tctatcaata cctgaaacag aacggccatc
      721 tcacggatga ccatcgggtg cggccccgca acaaattcaa ggtgccaggc atggccagac
      781 acgcgcgccg caaacatcag ctctccagcc tcgacgagct cagcgcccac aaggacaaga
      841 tcccggccct gctcaattcc tatctcaaag cgggggccaa agtctgcgga gtcccggcct
      901 tggaccgcag ctttaaatgt atcgactttc tcaccctgct gagggtgcag gatatgggcg
      961 ccggctacgg tcgcaaatac aacaaagatc attgaattca tgcgcctgac caaactaccc
     1021 cacctgatcg tcaacaccgc cgtcctggct acctatttcg gggtgggatt tgtgctgcag
     1081 tggatcaccc gcgatcccat caaacgtcgc cgcagggcta tccgcttcac ctccaccatg
     1141 tgcagggtct tcctcaaaac cctcaatatc gagatcaaag tggaaggccg ggaaaagctg
     1201 caagccctgc ataaatccag ctatctgatg gtgtccaacc actcttccta caccgatatc
     1261 ctcgtgcttt catcgctgga aaacctcgtc ttcatcacct cggtggagat gggcaacaac
     1321 cccgttttgg gagatatcac ccgcctggga ggctgcctct ataccgaccg caaaaaaccg
     1381 gtctccctgc ccaaagagat ccagcgcttt tccgccacaa tctctgaggg cttcaaggtg
     1441 gtgctcttcc cggaagggac cagtaccgat gcccgcagca tcaaagactt ccgcgccagc
     1501 ctctttcagg tggcgatcgg ggcccgggcc accatcctgc ccgtctgcat cagctatcaa
     1561 agcgtggacg gccagccctt tggagacaac aaccgcgaca ccatctgctg gtatggcgac
     1621 atggactttg tgccccactt catcggtttg ctcaatcacc agatcggggt gagggtcact
     1681 atcctggatc ccatcccctt cgatcccaat ctcagccgcg cggagctctc tgatctgacc
     1741 cgcgaggcac tgttaaagac ttatcacatc gatagataaa ggagtttatg aatggccccc
     1801 aaaaaagacc gactcggact tggcaagatc aacttcctgc tcctggccct ggcagccatc
     1861 ctgctgatcg cgggatactt tatcatgtcc ctcaacgaga tcaccatatc gccgcttatt
     1921 ttggcgggcg tgtatgtgct gttgatccct ttcgccctgc tctacaggcc caaacccaaa
     1981 gaataagacg cgatgcccat cctctccgat gagacggctg ccaaactaaa aagctgccag
     2041 ctcaacgcca aagccatcgt ggaaggcttc ctcgtggggc tccatcgcag cccctatcac
     2101 ggcttcagtg tggagttctc ggaccatcgg gaatacaacg agggcgaacc cctcaaagac
     2161 gtggactgga agatcgtggc caggacagac cgctactatg tgaaacgcta tgaggaagag
     2221 accaacctcc gctgctatat cctcatcgat cacagcaaga gcatgttctt ccaatccggg
     2281 gccctcacca agatcgcata cgccacccaa ctggcagccg cgctggcctg gctgatgatt
     2341 ggccagaagg acgccgccgg attgatcact ttcaacgatc acgtgaccgg ccaatacgcc
     2401 cccaaagcgt atcgcggcta cctgcccatc ctgttcaggg ccctggtgga actggagccg
     2461 ggaggggaga ccgatgtaag ctccgccctg cacagcatcg ctgaaaccat ccgcaaacgc
     2521 agccttatcg tgctgatctc cgacctcctg gacgaaccgg aaagcatcgc ccagggcctc
     2581 aaacactttc gggcccgcca ccacgaagtg ctggtgtttc acatcgcgga cctcatggag
     2641 gaaaacttca gcttcagacg ggagaccgag tttgttgaca gtgaaacggg agagaagctg
     2701 actgtcactc cctggcagat cagacagcga tatttagaga gctatggcca gcatctggaa
     2761 gcgttgaagg cccagttccg ccagttccag atcgaataca accccgtaag caccgccgag
     2821 cctctcaatg acctgctgct aaaatacctc ttaaagaggc gaaaaggatg aagtcaatat
     2881 ccgatgtgcc tgcaagggca aaaagctttt atacgcgatt tccggggcat ctatgagcgt
     2941 gacgcgcaga atcgacgctt atgatctctt tatggcagtc aagaacggac tcaatcagat
     3001 ctactgccaa ccggaccctg cggtgatctc taaaatcgcg gccctggcca aaaaggaaaa
     3061 gagccccctg gccaaagaca tgctgactgc cattaccgtg aacgcggaaa tcgctgcccg
     3121 ggaaggcata ccatgctgtc aggataccgg cacggtggtc ttttttatcg agctgggaga
     3181 gcgggccgtg ctcgtggatg gaacgataga aaacactatc aaagcagtgc ttagactggg
     3241 ctggtatcgc ttgaacctgc gtcgctcgct ggtggaagaa cccctctttg aacgccagat
     3301 cattccagat acagtgccgc ccgtgatcca ctgggagata aatcccggga atcagatcaa
     3361 ggtgaccatg ggactgaagg gtgggggagc ggagaacatg agcactctaa agatgatgcc
     3421 ggccacatcg acggtcgaac agatcaagca ggcggttgtg gagacggtag tgaatgcagg
     3481 gggaaaagca tgcccgcctg tgctcgtggg tgtagggatc gggggaaatt acgaaagctg
     3541 cgcggtgatg gccaaacgcg cgctctttga agaactgggc agagctcatc cggacccaag
     3601 atacgctact ctggaaaagg agattctggc cgagattaac cgtcagggga gaggtgtgat
     3661 gggaatgggg gaggggccca gcgctctggc agtgcatatc aagacggccc cttgccacat
     3721 cgcttcatta ccgctggcgg tcaatctgga ttgtcacgcc caccggcatt acagttttac
     3781 cctctaggtt tcacgtgaaa catcggcttt gcttatgaac atccacaagc tcaggcttcc
     3841 cctcgctact catagcagtt ccactctcaa agcaggtgac aaggtcctgc tctcaggtac
     3901 tctttatact gccagagata tggcccataa acgtctggcc gctctcattg cggcaggaaa
     3961 tcccctgccc tgggacctct cagagggagc cctcttttat tgcggtcctt cacccgttcc
     4021 caaaggcaaa atctgcgggg cgatcggacc caccacctcg gcacgcatgg accccttcac
     4081 gccattgctc ctggagcacg ggctcagggt catgctggga aaaggagaac gctcaccgga
     4141 agttcaaaag gccattcgcc accatggagc tatctatctg gcggccgtgg gagggatctc
     4201 agccctgctg gcccaacatg tgatcaagtt tgagctcttc ctgtggcccg agctgggagc
     4261 cgaagcggtg tacagaatgg aggtggagga ttttccctgc tatgtgatga ctgtgtgagt
     4321 ctttactacc gagctgcaga gggttgtctt tctcactatt gagatacaga ggtttttctc
     4381 tacagagatt cagaggcagc agagagaggc aaagaggggg cttgataagt gagaattgat
     4441 cgttaacaat tgattgaaac acgaggcatt ggagccggaa acttcctcgt ttccggagcg
     4501 tggaaatacc agtggcaaag caatcaatcc tgtgaag
//
LOCUS       DAOSFN010000170         4536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_17138_length_4536_cov_8.807632, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000170 DAOSFN010000000
VERSION     DAOSFN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17138_length_4536_cov_8.807632"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1283
                     /locus_tag="PLO35_08935"
     CDS             198..1283
                     /locus_tag="PLO35_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOH79834.1"
                     /translation="MSYKIKRAKEIVLVFVALPLLLALVVIVLIAIRQKLFEKKYLFH
                     TSLQNAIGLSDQTPVLYKGFEIGRVSSFYLENDGRIKVEFKVLKRYRDQLVANSVIFR
                     STNPIINTTTLEYLRDPVSKRLLPDGSTIPSTDFADGKAIVEALGLSGSDPVGGILES
                     VTRISSELAGSTGQKSALLRTIDNIALASDKAEDMLIMVESSLAELNSFAANLNRDNN
                     SNAGAVFRAINNLADLSGQVNDQMGVMKELLTTGKDLLATYSEPDSLVARMIDPSGEN
                     LFKPIRATLEGLSANLEASLRLLNSFNASSPEIASLLSGLNDSLADARRTLEALNNNP
                     ILKMGMKPSGFEPAYPVDRIGEMPLAP"
     gene            1273..2127
                     /locus_tag="PLO35_08940"
     CDS             1273..2127
                     /locus_tag="PLO35_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79835.1"
                     /translation="MRHSLILILSLLSVACSWSHKPLQPLEREAADRALRTAGTQLHN
                     DPASASGTYLKALKQYRAMADIEGELWSLSGIAVSSLALKDSLAYLDSRERMDDIVKE
                     ISPERDYIPLLLELQLAAASSDWILVSELYRDNEDWPSVARLRILAYAIQAESLLGQD
                     RSAHVKLALKLLRGQKRKLHRQGDLHALQIARTRYALAYHYLVETDLKKAEKQLNQAL
                     ELDRLYGDFDALGYDYWLLARIAIARGDPTTARAQLLKAQRLFEGSAHAAMQTRVATE
                     LLSLEKGE"
     gene            2128..2892
                     /locus_tag="PLO35_08945"
     CDS             2128..2892
                     /locus_tag="PLO35_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOH79836.1"
                     /translation="MDLRGKRALILGIANQHSIAYGIAKALHEQGVELIFSYAGPMLA
                     KRVFPIAEELGAKLCVECDVSKDYSIQKLMDQVRAHWDGIDMIVHSVAYAPTDELNKA
                     FHLTSRQGFCTAMDISVYSLVAVIREAGELLAPGASVITLSYYGAQKVVPNYGVMGVA
                     KAALEASVRYLAFSLGEQGVRVNAISAGPLRTLSAAAISGLSQIQRRIEKSAPLGRNI
                     DPEDVAKTALYLLSDLSSGVTGEVIFVDAGMNIMAY"
     gene            2905..4170
                     /locus_tag="PLO35_08950"
     CDS             2905..4170
                     /locus_tag="PLO35_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH79837.1"
                     /translation="MKLFTNASLPTSSNALKRVNILFDETIKKVSVNPIETEEPCEEI
                     DLGGRIVLPGAIDAHCHIVDSVEPSEAISRLSRMALLGGWTTVSELSYFSPLPIFDLR
                     DFKHLQSFIDAASYVFMPLWANIEIENYPYHAEAALELWTRGALGLTIFSPSPSEALT
                     ELSFTEIMDLFIDIYESDTAFVFQGWDQEAYQSPSFEAQTDAIKKILRRMQENPIHVP
                     RVGSWATIEFINSISKRSDISFALNVMDLMDTFGVEGLPGREHDLEENKDLLSELIRT
                     NKLYMVSNHASLSDSQGDCVFRVETPGTMPYSYLWMLSEFWKKRKVPLATVIKMCSEN
                     AAKRLGIYPMKGSLDPGSDADFVIYNPDGITAMPLPEGGSIDLTGSIESIWLRGTLAV
                     QNGEVKERHGGFLARTNTPKRRHNNSTWI"
     gene            4161..>4536
                     /locus_tag="PLO35_08955"
     CDS             4161..>4536
                     /locus_tag="PLO35_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOH79838.1"
                     /translation="MDIEVVKLYSFKELEVWRRGIDLVEDVFTITSGFPTNELFTLVP
                     QMRRAAISIPSNIAEGQGRKNPREYIQFLYIAKGSLAEIETQLIICERLTLIDDLNDM
                     TEKIKIIRMMLIGLINKLNQPQN"
BASE COUNT         1103 a         1267 c         1182 g          984 t
ORIGIN      
        1 aatcgtgtaa atcctgtcat cctgtcatct gcgttctata taaaattatc tgtgtaaatc
       61 ccttttttcc ctatttctgc gttctattaa ttctcaattc tcaatcctca attctcaatt
      121 aaagaggtgc ggcatataaa ccttgacaga tttgcaaggg cagaaaatca taaccgttat
      181 ctggagaaaa ctgcctaatg agctataaga taaaacgcgc caaagagata gtgctggtgt
      241 tcgtggcttt gccactgttg ctggcactgg tggtgatcgt gctgatcgcg atccggcaga
      301 agctgtttga gaagaaatat ctctttcaca ccagcctcca gaacgccatc ggcctatccg
      361 atcagacccc tgtgctctac aagggctttg agatcggcag ggtgagcagc ttttatctgg
      421 aaaacgatgg ccgcataaag gtggaattta aggtgctaaa gcgttaccgg gaccaactgg
      481 tggccaattc cgtgatcttc cgctccacca atcccattat caataccacc actttggaat
      541 atctgcgtga tcccgtctca aaacgtttac tgccagacgg ttccaccatc ccgtccaccg
      601 atttcgcaga cggcaaggct atcgttgaag ccttgggact cagcggcagc gatcccgtgg
      661 gcggcatcct ggaaagcgtc accaggatca gctcggaact ggctggttcc actggccaga
      721 aaagcgcttt actgaggacg atagacaaca ttgccctggc cagcgacaaa gccgaagaca
      781 tgctgatcat ggtggaaagc tctctggcag agctaaatag cttcgctgcc aacctcaacc
      841 gggacaacaa ctccaatgcc ggagcggtgt ttcgggccat caataacctc gccgatctga
      901 gcgggcaggt gaatgatcag atgggggtga tgaaagagct gctgaccacc ggaaaagacc
      961 tgctggccac atattcggag ccggacagtc tggtagcccg gatgatcgat cccagcggag
     1021 aaaacctctt caagcccata agggccactc tggaaggcct gagcgctaat ctggaggcca
     1081 gcctcaggtt gctcaacagc ttcaacgcca gttctccaga gatcgccagc ctgctgagcg
     1141 gcctcaacga cagcctggca gacgcgcgcc gcactctgga agccctgaac aacaacccca
     1201 tcctgaaaat gggaatgaag ccttccggtt tcgagcccgc ttatccggtg gaccggatag
     1261 gagaaatgcc gcttgcgcca tagcctgatc ctcatcctca gcctcctgtc agtggcctgt
     1321 tcgtggtctc ataaaccgct gcaacccttg gaacgtgaag ccgcggaccg ggccctccgg
     1381 actgctggaa cccagctgca caacgatccg gccagcgctt ccgggactta tttgaaggcc
     1441 ctcaagcaat atcgcgccat ggctgatata gaaggcgagc tgtggtccct gagcggaatc
     1501 gccgtttcca gcctggcttt gaaagacagc ctggcatatc tggacagccg tgagcgcatg
     1561 gatgatatcg tgaaagagat ctcccctgag agggattata tccctctgct gctggaactt
     1621 cagctcgcgg ccgccagttc ggactggata ctagtctcag aactttatcg ggacaacgaa
     1681 gactggccat ctgttgccag actgagaatc ctggcctacg cgatccaggc ggaaagcttg
     1741 ctgggacaag acaggtccgc ccatgtcaag ctggccctca agctgctgcg gggccagaag
     1801 cgcaagctgc accgtcaggg agatttgcac gccctccaga ttgctagaac gcgttacgcg
     1861 ctggcctatc actatctggt cgaaacagac ctaaaaaagg ccgaaaaaca gctgaatcaa
     1921 gccctcgagc tcgaccgcct ctatggcgat ttcgacgctc tgggctatga ctattggctg
     1981 ctggccagaa tcgctattgc ccgtggcgac ccgactacgg ccagggctca actgctgaaa
     2041 gcacaacgtc tttttgaagg ttccgcccat gccgcgatgc agaccagagt ggcaacggaa
     2101 ctcctgtctc tggaaaaagg agaataaatg gatcttagag gaaaacgcgc cctcatattg
     2161 ggcattgcca atcaacactc catcgcctac gggatagcca aagcccttca cgaacaggga
     2221 gttgagctga tcttcagtta tgccggaccg atgcttgcca agcgagtctt cccgatcgcg
     2281 gaagagctgg gcgccaagct ctgtgtggag tgcgacgtct ccaaggacta ctccatccaa
     2341 aagctgatgg atcaggtccg cgctcactgg gacgggatcg acatgatcgt ccacagtgtg
     2401 gcctacgctc ccacagacga gctcaacaaa gcctttcatc tcaccagccg gcaaggtttt
     2461 tgtacggcga tggacatcag cgtctattca ctggtagcgg tgatccggga agcgggagaa
     2521 cttttggctc ccggggccag tgtgatcacc ctcagctatt acggggccca gaaagtggtg
     2581 cccaattatg gagtgatggg agtggccaaa gccgctctgg aagccagcgt gcgctatctg
     2641 gccttcagcc tgggagagca gggcgtgagg gtaaacgcca tctcggccgg tccgctcaga
     2701 actctctcgg cggcagcaat ttccggcctt tctcagattc aacgccgcat cgagaaatcc
     2761 gctcccctgg ggcgcaacat cgatccggaa gatgtagcta aaaccgctct ctatctgctt
     2821 tccgacctct catcgggtgt gaccggagaa gtgatctttg tggatgccgg catgaacatc
     2881 atggcctact gaagaagcgc ctccatgaag ctgtttacaa acgcctcgct gcctaccagt
     2941 tccaacgccc tcaagagagt caacatcctc tttgacgaaa cgatcaaaaa ggtctccgtc
     3001 aaccccatcg aaaccgaaga accctgtgaa gagatcgatc tgggtggcag gattgtgctg
     3061 cccggtgcca tcgatgcgca ttgtcacatc gtggacagcg tcgagccctc ggaagccatc
     3121 tcccgcctga gcaggatggc cctgttggga ggctggacca ccgtgagcga actgagctat
     3181 ttcagtccgc taccgatctt tgacttgagg gatttcaaac atctgcaatc ctttatagac
     3241 gcggcttcct atgtgtttat gccgctctgg gccaatatag agatcgaaaa ctatccctac
     3301 cacgccgagg ccgctttgga gctgtggacc agaggcgcgc tgggactcac catcttttct
     3361 ccctctccct ccgaagcgct tacagagctt agctttacgg agatcatgga cctctttatc
     3421 gatatctacg agagcgacac cgccttcgta tttcagggct gggaccagga ggcttaccag
     3481 agccccagct tcgaggccca gaccgatgct atcaaaaaga tcctcaggcg catgcaggag
     3541 aaccccatcc acgtacccag ggtgggctcc tgggccacca tcgagtttat caacagcatc
     3601 tccaagcgct ctgatatctc attcgcgctc aacgtgatgg acctcatgga tacctttggg
     3661 gtggaaggcc tgcccggccg cgaacacgac ctggaagaaa acaaagacct gctgagtgag
     3721 ttgatccgca ccaacaagct ctacatggtc tccaatcatg ccagcctctc tgactcgcag
     3781 ggtgactgcg ttttcagagt tgaaactccc ggcaccatgc catactccta tctgtggatg
     3841 ctttcggagt tttggaaaaa acgtaaagtg ccgctggcca cagtgatcaa gatgtgcagc
     3901 gaaaatgccg ccaagcgctt gggtatttac cccatgaagg gttccctgga ccccggttcg
     3961 gatgccgatt ttgtgatcta taaccccgat ggaattaccg ccatgcctct tccagagggt
     4021 ggcagcatcg atttgaccgg cagcatcgaa tccatctggc tgcgcggaac gctggcggtt
     4081 caaaacggcg aggtcaaaga gcgccacggt ggatttctgg ccaggaccaa cacccccaaa
     4141 cgcagacaca acaactcaac atggatatag aggttgtaaa gttgtatagt tttaaggagt
     4201 tagaggtttg gcgccgcggc atagacctgg tggaagatgt gtttactatc acgtccggat
     4261 ttcctactaa cgagctgttt acgctggtcc ctcagatgag gagggcagcg atttcaatac
     4321 catcaaatat tgccgaaggc caggggcgca agaatcccag ggaatatatt caattcttat
     4381 acattgccaa aggctctctg gccgaaattg agacccaatt gatcatttgc gagagactca
     4441 cattgattga tgacctgaat gatatgaccg aaaagatcaa gatcatcaga atgatgttga
     4501 ttggtctgat caacaagctc aaccagcctc aaaacc
//
LOCUS       DAOSFN010000171         4467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_17571_length_4467_cov_7.434497, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000171 DAOSFN010000000
VERSION     DAOSFN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17571_length_4467_cov_7.434497"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLO35_08960"
     CDS             complement(<1..661)
                     /locus_tag="PLO35_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79839.1"
                     /translation="MVKHIFSIILMIALLLSACAKDKEKPVQPARDGSFADTQQRSGQ
                     LFNELDGKEGAAPQSSSPGEQPIPSSSATEVAPNVVVSTTAASPAPKPGEAIVVEIDS
                     FKVFSDTVSPNEAKIQTRNMMRELALEKALPSDVSITGMSTSMYVERNNKFDESTAKS
                     IFMMSSSAGRFLEEKLLKAEPSFDTSTSSLTYRMRYRAKILPLEKTYNPSMSLQVKIS
                     NT"
     gene            complement(655..>2670)
                     /locus_tag="PLO35_08965"
     CDS             complement(655..>2670)
                     /locus_tag="PLO35_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH79840.1"
                     /translation="GEFETNAMFEQRKKDMEASKTAIQKEYAQKIADARAAHDAQLAK
                     LRQRLNQLLVSSRETVTLAGSLGKYDPENQRFPVSIPEKTFQITVPLSKGPEVKDNIG
                     KYRFEVTRQLNQNLAWDYLEARLVGPGGTFASTDKAPAIGQSSTTVALIPPDLSATVT
                     FSEPSGNKMLDSEETAQVVIAVKNTGKGSGNMVEASFELLNAQGVSFSRSLYFGEIKP
                     GETVNKTLQLIAGMDTKDAQAELKIGFKEQNGFPPNDMVLRFNTKALQAPDIYIADIG
                     IEDFNKNNKIEPGEQVEVKVRIHNRGQGLARNLSADLDLGKDVYLFANSSGSFNLGDM
                     ASGAYKDLVFSIVTAKTATNLDIKLNLKESRAQFSKSAQPLNLAFNRVERTADQLLVT
                     GTQSSAQVAAAPALSIDVEQDIPVRGKSKKHRWGVIFGIETYRNVPPVRFAKRDAETM
                     RDYFTKVLGIPSENLYLKTNEEAGLSEFKTVFDPQGWLSKNAGAKDSEIYIYFSGHGV
                     PTPDGKQAYLLPFDGNPNYASNSAYDLEQLYKNLGAIKAKSITLFLDSCFSGANRDNE
                     IILADARPVFLSAAPKLTEAHLAVFSAASGAQISSAWADKQHGLFSYFLMKGLKGEAD
                     ANLDNKVTQSELNEYIGSQVSSQARRMGREQEPGLQSGDATRVMVQW"
     assembly_gap    2671..2770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3013..3546)
                     /locus_tag="PLO35_08970"
     CDS             complement(3013..3546)
                     /locus_tag="PLO35_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOH79841.1"
                     /translation="MDEYIRYTDTLRLAEKQKPEDHELKATNNKKAFAFIDDIFHKSA
                     GGIDFTKPEAIATMMSENLQALHGKLCFISAYTIMPNHLHILLRPFEREGKPISTAEI
                     MKSFKGLSARQVNLILNRSGSLWYREYYDHWVRSGREFVNVVEYIRQNPVRAGLVKEP
                     EEWKWTWLNLHEGEVMW"
     gene            complement(4115..>4467)
                     /locus_tag="PLO35_08975"
     CDS             complement(4115..>4467)
                     /locus_tag="PLO35_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH79842.1"
                     /translation="NKSKGYKYSGSNDVGSVAWYDSNSGSKTQAVGTKQANEIGIHDM
                     SGNVWEWCWDWYDDYASASQNNPRGATNGSSKVLRGGSWNLNVNFCRVSLRFDFDPGF
                     SFGSIGFRVSRAIW"
BASE COUNT          894 a         1185 c         1124 g         1164 t
ORIGIN      
        1 gcgtattgct gatcttcacc tgcaaagaca tggagggatt gtaggtcttt tccagcggta
       61 agattttggc gcggtagcgc atgcggtatg tgaggctgct ggtgctggtg tcaaagctgg
      121 gctcggcttt caggagcttt tcttccagaa agcgccccgc cgaggatgac atcataaaga
      181 tgctcttggc agtgctttcg tcaaacttgt tgttgcgctc cacatacatg ctggtggaca
      241 tccccgtgat cgagacatcc gagggcaggg ctttctcgag cgccagctcc ctcatcatat
      301 tgcgggtctg gatcttggcc tcgttggggc tcaccgtgtc gctgaacacc ttaaagctgt
      361 cgatctccac cacgatcgcc tctcccggtt tgggcgcggg gcttgcggct gtggtgctaa
      421 ccaccacgtt gggagccact tccgtggcgc tggagctggg gattggctgc tcccctgggc
      481 tgctggattg gggagcggca ccttctttgc cgtccaactc gttgaacagc tgacccgagc
      541 gctgctgcgt atcagcaaaa ctgccgtctc tggctggctg gacgggcttc tctttgtctt
      601 tggcacacgc gctcaggagc agcgcgatca tcagaatgat tgaaaagatg tgttttacca
      661 ttgtaccatc acccttgtgg cgtcgccact ttgcaagccg ggttcctgtt ccctgcccat
      721 gcgtctggcc tgggagctca cctggctgcc tatatattcg tttaattcgc tttgcgttac
      781 cttattatcg aggttggcat ccgcctcgcc tttcaagcct ttcatcagaa agtagctgaa
      841 gaggccatgc tgtttatctg cccaggcgct ggaaatctga gctccgctgg cggctgaaaa
      901 cacggccagg tgggcttccg tgagcttggg agcggcgctc agaaacacgg gacgcgcgtc
      961 tgccaaaatg atctcgttgt cccgattggc cccgctgaaa cagctatcca ggaagagggt
     1021 aatgctcttg gccttgatcg ctccgaggtt tttgtaaagc tgttccagat cgtaggcgct
     1081 gttggaggca tagttgggat tgccgtcaaa gggcagcaga taagcctgtt tgccgtccgg
     1141 agtgggaact ccgtggccgc tgaagtagat atagatctcg ctgtctttgg ccccggcgtt
     1201 cttgctgagc cagccctggg gatcgaagac ggtcttaaat tcactgagtc cggcctcttc
     1261 gttagttttt aggtagaggt tttcggacgg gatgcccagc acttttgtaa agtagtcccg
     1321 catcgtctcc gcgtcccgtt tggcaaagcg cacggggggc acattgcggt aggtctcgat
     1381 gccaaagatc acgccccagc ggtgcttttt gctctttccc cgcaccggga tgtcctgctc
     1441 cacatcgata ctgagagcgg gagcggctgc cacctgagcg gaactctggg tgccggtcac
     1501 caaaagctgg tccgcggtgc gctccacccg gttgaaagcc aggttgagag gttgggcgct
     1561 tttactgaat tgggcgcggc tctccttcaa attcagttta atatcgaggt ttgtggctgt
     1621 cttagcagtc acgatcgaaa acacgaggtc tttgtaggca ccggaggcca tatcgcccag
     1681 gttgaagctg ccggagctgt tggcaaacag atagacgtct ttgcccagat ccaggtcagc
     1741 gctcaggttt ctggccaggc cctgaccgcg gttgtggatt ctcactttga cctccacctg
     1801 ctcgccgggc tcgatcttgt tgtttttatt gaagtcctca atgccgatat cggcgatgta
     1861 gatgtcagga gcctgcagtg cctttgtgtt gaagcgcagc accatatcgt tgggcgggaa
     1921 gccgttttgc tctttgaaac cgatcttcag ctccgcctga gcatctttgg tgtccattcc
     1981 agctatcaat tgcaaagtct tgttcaccgt ttcacccggc ttgatctcgc caaaatagag
     2041 actgcggctg aagctgactc cctgggcgtt gagcagttcg aaactggctt ccaccatgtt
     2101 gccgctgccc ttgccagtat ttttaacggc aatcaccacc tgggcggtct cttcggaatc
     2161 cagcatcttg ttgccgctcg gttcactaaa cgtgaccgtg gcgctcaggt ctggagggat
     2221 taaagctaca gtcgtgctgc tttgaccaat cgcgggagcc ttgtctgtgg aggcaaaagt
     2281 gccacccgga cccacgagtc tggcctccag ataatcccaa gccaggtttt ggttgagctg
     2341 gcgggtgacc tcgaatctgt atttgccgat gttgtccttc acctcagggc ctttactgag
     2401 gggaaccgtg atctggaagg tcttttcggg tatggacacg ggaaatctct ggttttcagg
     2461 atcatacttg cccaggctgc cagccagggt gaccgtctct ctgctgctga ccagcagttg
     2521 attcagtctc tgcctcagct tggcaagttg ggcgtcatga gcggctctgg cgtcggctat
     2581 cttctgagca tattctttct ggatggccgt tttgctggct tccatgtcct tcttgcgctg
     2641 ttcaaacatg gcattggtct caaactcacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn tcgccgagaa tggcgcgtcc aaccagcttt tgagggctcg ggcctcgtcc
     2821 atcatagcag aaaacaacat tcctaccact actaccatcc aaatcaccag gcacacctgc
     2881 ttcatgatat cctcccgtat attacatcat aacaaatact tctaactttc acttcgttgc
     2941 cgtttacacc cattgtatca ctggcttcag ccggtcgtag cactgacttt agtcggtgcg
     3001 tgcaagccca tctcaccaca tcacctcccc ctcatgcaaa ttcaaccacg tccacttcca
     3061 ctcctccggc tccttcacca atccagctcg caccgggttc tgcctgatat actccaccac
     3121 gttcacaaac tccctaccgc ttctcaccca atgatcatag tattctctat accaaaggct
     3181 accgctcctg ttcagaatca aattcacctg cctcgccgac aggcccttga aactcttcat
     3241 gatctcagcc gtgctgatcg gctttccttc acgctcaaaa ggcctcagca ggatgtgcag
     3301 atgatttggc atgatcgtgt atgctgagat aaagcatagc ttcccatgca gggcctggag
     3361 gttttcggac atcattgtag ctatagcttc cggctttgtg aagtctatcc ctccagcaga
     3421 tttatggaag atatcatcta tgaacgcaaa ggctttcttg ttatttgtgg ctttcaattc
     3481 gtggtcctca ggcttctgct tctcggcaag tctgagcgta tcggtatagc ggatatactc
     3541 atccatgaat ttcggtggta tcggatcaga gctgcgcacc gtgataaaca gcggtcttcc
     3601 tttgggttgg atgtgaggta ggaagcgttt gtggtagaga gcgtagtcaa gcatgaagct
     3661 gagtcccctt cacgcaccga ctgaagtcag tgctacgacc ggctgaagcc agtgatacaa
     3721 agtttattct tgatttgatc caaccgccca gttgtttgcc gatgttgtcc atttgttcta
     3781 tcatggcatg atggaccttg ttatcgaaga ggtgcatatc atgtgatatt cgaataagca
     3841 gtctcagatg ttccagctta aaacttgcct ctgtgagaaa tccttgcttg tttgtggcaa
     3901 gatagcactt ttgcaataac tcgagcaatt catatatcgc gttttcaatt ctaagaccaa
     3961 gggagtatct atagtttctg ggatatcttg ccacaatggg caatagccac ttaacataat
     4021 tataacagtc attgacaatc ttggcctcat tcataaaaaa ttcgaccgcc tccggcggta
     4081 ccaaaggcca aagagccaaa gggccaaagt ccaattacca gatagcacga gaaacccgaa
     4141 agccgatgct gccgaagctg aagccgggat cgaagtcgaa gcgaagagac acgcggcaga
     4201 agttgacatt gaggttccag ctaccgccac gcagcacttt actggatcca tttgttgctc
     4261 cacggggatt attttgtgaa gcacttgcat agtcgtcata ccagtcccag caccattccc
     4321 aaacgttgcc gctcatatca tggatgccga tttcgttggc ttgcttcgtc ccgacagcct
     4381 gagttttgct tcctgagtta ctatcatacc atgccacaga gccaacatcg ttggagccgc
     4441 tgtacttata gcccttgctc ttgttgc
//
LOCUS       DAOSFN010000172         4425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_17806_length_4425_cov_9.634096, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000172 DAOSFN010000000
VERSION     DAOSFN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17806_length_4425_cov_9.634096"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2552)
                     /gene="clpB"
                     /locus_tag="PLO35_08980"
     CDS             complement(<1..2552)
                     /gene="clpB"
                     /locus_tag="PLO35_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOH79843.1"
                     /translation="MNTQRFTIKSQEALQAMQNLATEHGHQEIGVLHLLAAMLQQPDT
                     LIVPVLQKLEIDPGNINSQVEDALRKLPRVSGAQVYLAQPVLDVLHQAEREADQLGDD
                     YISLEHILLALVSKGGEAGKILTGKGLDHATLMRALKELRGNQRVTDQNPEAKYQALD
                     KYARNLTRLARQEKLDPVIGRDEEIRRGIQILSRRRKNNPILIGEPGVGKTAIVEGLA
                     RRIVARDVPENLKDKELVELDMAALLAGAKFRGEFEERLKAVLAEVEKSDGRIILFID
                     EIHTVVGAGAAEGAVDASNMLKPALARGSLHCIGATTIDEYRKHIEKDPALARRFQPV
                     MVQEPSVEESISILRGIREKYEVHHGVKISDKALVAAAVLSDRYISDRFLPDKAIDLM
                     DEACAQLRMEIDSLPVELDEAERKLRQLEIEKLSLAKESDAMSASRLEKISSEIARYQ
                     ESRNTLRLKWENEKKIFEQERALRAEIDALKGQADLAERNGDLETTAKLRYGLIKDKE
                     RELETLAAAKGGPEGYEPLIGEQVDEELVAQVVAKWTNIPVSKLLQSEMAKLLTLEDT
                     LAQRVVGQKEGITALANAIRRSRSGLGDGNRPIGSFIFLGPTGVGKTELAKTLASYLF
                     DSEKAMLRLDMSEFMEKHSVSRLIGAPPGYVGYDEGGYLTEGVRRRPYSVILFDEIEK
                     AHPDVFNVLLQILDDGRLTDGKGRTVDFRHSVIIMTSNIGSQMIYDTAHEDLDELRPE
                     LMNELKRYFRPEFLNRLDDIIIFHRLGRADIRAIVEIQLNVLAKRLQTKGIELSWTDA
                     LAQHIAAAGFDPQFGARPLKRLIQKDIEDALARALLGGEISAGNPVRLDYDGS"
     gene            2732..4408
                     /gene="ettA"
                     /locus_tag="PLO35_08985"
     CDS             2732..4408
                     /gene="ettA"
                     /locus_tag="PLO35_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOH79844.1"
                     /translation="MEDNKVIYSMSGIGKVVDGKRWILKDIWLGYYYGAKIGVVGLNG
                     SGKSTLLKIMAGLDTDFIGQVAMSKGYSVGYLEQDPQLDPSRTVLEIVQEGVAETVAL
                     LKRFDEINNRFAEPMSDDEMSALMEEQGKVQDALDAANAWDLDSRLELAMDALRCPPS
                     DLPVSVISGGERRRVALCRLLLQEPDILLLDEPTNHLDAETVLWLERHLKQYKGTVIS
                     VTHDRYFLDNVAGWILELDRGEGIPYEGNYSSWLEQKQIRLAQEAKGESRRQKALADE
                     LEWIRSSPKARQAKSKARIQNYEKLASQEYRRENTTDQIVIFPGPKLGNKVIIASDVS
                     KAYGDKILYEDLSFNLPPGGIIGVIGPNGAGKTTLFEMIAGKIQPDSGTFTLGETVKL
                     SYVDQARVMDTDKTLLELIADGRDVLKIGDREINAREYISKFNFGSKEHIKPMNILSG
                     GEKNRAYLALTLQTGGNVLLIDEPTNDLDVYTIHALEEALLDFAGCAVVISHDRYFLD
                     RVCTHILAFEGESSVKWYEGNFSDYEEDKLRRLGEKAALPKRITYRRLTR"
BASE COUNT          895 a         1302 c         1243 g          985 t
ORIGIN      
        1 gagccgtcgt aatccagccg taccggattg ccagcactga tctctccgcc cagcagagct
       61 ctggccagag catcctctat atctttctgg atgaggcgtt tgagcggcct ggcgccaaac
      121 tggggatcaa atccggcagc ggcaatatgc tgcgccaggg catccgtcca gcttagttct
      181 atccctttgg tttgcagacg tttggccagc acgttgagct ggatttccac gatggcgcgg
      241 atgtctgctc tgcccagccg gtggaagatg atgatatcgt ccaggcggtt cagaaactcc
      301 gggcggaaat agcgcttgag ctcgttcatg agctccggac gcagctcgtc gaggtcttca
      361 tgcgcggtgt cgtagatcat ctgggagccg atattggagg tcatgatgat cacgctgtga
      421 cgaaagtcca ccgtgcggcc cttgccgtcc gtgagtctgc cgtcgtcgag aatctgcagc
      481 agcacgttaa acacgtccgg atgggctttc tcgatctcgt caaagaggat gacgctgtag
      541 ggacggcgcc gcactccttc cgtgaggtag ccgccttcgt cgtaacccac atatccggga
      601 ggagctccga tcagcctgga aaccgagtgc ttttccataa actcgctcat atccaacctc
      661 aacatggctt tttcgctgtc gaagagatag ctcgccaggg tcttggccag ttccgtcttt
      721 cctactcccg tagggcccag gaagatgaag gagccgatgg gacggttgcc gtctcccaaa
      781 ccgctgcggg agcgtcggat ggcattggcc agcgcggtga tgccttcctt ctgccccacc
      841 actctttggg caagggtatc ctcgagggtg agcagcttgg ccatctcgct ctgcagcagt
      901 ttggagaccg gaatgttggt ccatttggcc accacctggg ccaccagttc ctcatccacc
      961 tgctcgccga tcagaggttc atagccttcc ggaccgcctt tcgcggctgc cagcgtctca
     1021 agctcccttt ccttgtcttt gatcaggccg taacgcagct tggcggtggt ttcaaggtcg
     1081 ccgtttcgct ccgccaggtc ggcctggcct ttgagcgcgt cgatctctgc tctcagggca
     1141 cgttcctgct caaagatctt cttctcgttt tcccatttca gcctcaaagt gttgcggctt
     1201 tcctgatagc gcgcgatctc agagctgatc ttttccaggc gggaagcaga catggcgtcc
     1261 gattccttgg ccagcgagag cttttcgatc tcgagttgac gcagcttgcg ctccgcctca
     1321 tccagttcca cgggcaggga atcgatctcc atgcgcagtt gcgcgcaagc ttcatccatc
     1381 aggtctatcg ccttgtccgg caagaacctg tctgagatat agcggtcgct gagcaccgcg
     1441 gcagccacca gggctttgtc tgagatcttg acgccgtggt gtacctcata cttttcgcgg
     1501 atgcccctca ggatggagat gctttcctcc acgctgggtt cctggaccat cactggctgg
     1561 aagcgtctgg ccagagcggg gtccttttct atgtgcttgc ggtattcgtc gatggtcgtg
     1621 gccccgatgc agtgcagcga acctctggcc agagcgggtt ttagcatgtt ggaagcatcc
     1681 accgctcctt cagccgcgcc ggcacctacc acggtgtgga tctcgtcgat aaacaggata
     1741 atcctgccgt cgctcttttc cacttccgcc aatacggctt taaggcgctc ctcaaattcg
     1801 cctctgaact tggcacccgc cagcagggct gccatatcca gctccaccag ttccttgtcc
     1861 ttgaggtttt ccggaacgtc gcgggccacg atcctgcggg ccagaccttc cacgatggcg
     1921 gttttgccca cccccggttc gccgatcagg atgggattgt ttttgcgccg tctggagagg
     1981 atctggatgc cacggcggat ctcctcgtcg cgtccgatca cggggtccag tttttcttgc
     2041 cgggccaggc gggtgagatt gcgggcatat ttatccaaag cctgatattt ggcctcggga
     2101 ttctggtccg tgacccgctg gttgcctctc agttctttga gggcgcgcat cagggtggcg
     2161 tggtccaggc ctttacccgt cagtatcttt ccggcttctc cacccttgct cacgagggcc
     2221 aggaggatgt gctcgaggct gatgtaatca tcaccgagct ggtccgcttc ccgctcggcc
     2281 tgatgcaaaa cgtccagcac cggctgagcc agatagacct gcgctccact cactctgggc
     2341 agcttgcgca gggcgtcttc cacctgggaa ttgatgttcc cgggatcgat ctccagcttt
     2401 tgcagcaccg gaacgatcag ggtatccggc tgctgcaaca tggccgccag cagatgcagg
     2461 actccgatct cctgatggcc gtgttcggtg gccagattct gcatcgcttg cagggcttcc
     2521 tgacttttaa tggtaaatct ctgggtgttc atagtctcct ccttggggat gaactggttg
     2581 tttgagaaca aattatgcgg ggcccctgag gctgtcaaga gaaaattagc agactaatgt
     2641 acagactgct aatttccctc gccaattcct gcttgacact ccataccctt tttcaagcct
     2701 gtcctgtatc atctaaacac ttgaggaaca aatggaagac aataaagtta tctactcgat
     2761 gtccggcatc ggcaaggtgg tggacggcaa gcgctggatc ctgaaagaca tctggctggg
     2821 ctattattac ggtgccaaga ttggcgtggt gggactcaac ggttccggca aaagcaccct
     2881 gctcaagatc atggcgggcc tggataccga tttcatcggc caggtggcga tgtccaaggg
     2941 ttacagcgtg ggctatctgg agcaagatcc ccagctcgac ccctccagga ccgtcctgga
     3001 aatcgtccag gaaggcgtgg cggagacagt tgcccttctg aaacgctttg acgagatcaa
     3061 caaccgcttc gcggaaccca tgagcgacga cgagatgagc gctctcatgg aagaacaggg
     3121 taaggtgcag gacgctctcg acgcggccaa cgcctgggac ctcgacagcc gtctggagct
     3181 ggccatggat gccttgcgct gtcctccctc agacctgccg gtgagcgtga tctcaggtgg
     3241 agaaagacgc cgtgtggcct tgtgcagact gcttttacag gagccggaca tcctgctgct
     3301 ggacgagccc accaaccatc tggacgccga aaccgtgctc tggctggagc gccatctcaa
     3361 gcaatataag ggcaccgtga tctccgtgac tcacgaccgc tatttcctcg ataacgtggc
     3421 cggctggatc cttgaactcg accgtgggga aggcatcccc tatgaaggaa actactccag
     3481 ttggctggaa caaaagcaga tacgcctcgc tcaggaggcc aagggtgaaa gccgcaggca
     3541 gaaagccctg gccgacgaac tggaatggat ccgcagcagt cccaaagctc gccaggccaa
     3601 gagcaaagcc cgcatccaga actatgaaaa gctggcttct caggaatacc gcagggagaa
     3661 tactaccgac cagatagtca tcttccccgg ccccaagctg ggcaacaagg tcatcattgc
     3721 gtcagacgtc tccaaagctt acggcgacaa gatattgtat gaagacctca gcttcaatct
     3781 ccctcccggt ggcattatcg gtgtgatcgg tcccaacgga gcgggcaaga ccaccctctt
     3841 tgaaatgatc gccggcaaga tccaacccga tagcgggaca ttcacactcg gcgagacggt
     3901 caagctcagt tatgtggacc aggcgcgcgt gatggatacc gacaaaacct tgctggagct
     3961 gatcgcggac ggacgtgacg tcctcaaaat cggcgaccgc gagatcaacg cccgcgaata
     4021 catcagcaag ttcaactttg gcagcaaaga gcatatcaaa ccaatgaata tcctctctgg
     4081 gggcgaaaag aaccgcgcct acctggccct cacactccag acgggtggca acgtcctcct
     4141 catcgacgag cccaccaacg atctggacgt ctataccatc cacgccctgg aagaagctct
     4201 cctggatttt gccggctgcg cggtggtgat cagccacgat cgctatttcc tggatcgggt
     4261 ctgcacccac atcctcgctt tcgagggtga aagcagcgtc aaatggtatg aaggcaactt
     4321 cagcgattac gaggaagaca agctcaggcg attgggtgaa aaggcggccc tgcccaagcg
     4381 gatcacttat cggaggttga cgaggtgagc tgcgctggtt agagc
//
LOCUS       DAOSFN010000173         4425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_17807_length_4425_cov_7.572082, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000173 DAOSFN010000000
VERSION     DAOSFN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17807_length_4425_cov_7.572082"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..2516)
                     /locus_tag="PLO35_08990"
     CDS             complement(132..2516)
                     /locus_tag="PLO35_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79845.1"
                     /translation="MKKTSLILFLIALTSFLPGIVAIHEPPRTYVSGSSVDLSIEIPA
                     EVETIAKINLCYGPRGETGYSEKTMDSPLPGTQAFTVRLQPQELQDRELQYWFDFELT
                     DGSTFSYPDEALGGEPYTLKTEIQSGKEDVGFVLLSTEEDFAQKGEYVLVVSYFAIAD
                     QIDPATIEVWVNGRNVTSLAVITENLLSYRDPKPKTKNYEAVVTALDRQGKLIKSQVF
                     TTLVDPKGGRIKIYTWGGVNFASNFHRYRPQEEYEPNQPEDPKNSYAGWLDFNIRYKQ
                     LKLNLNALHSSLENPNRQPIDRYCAGLQLGFLTIQAGDQIPQLSPLAMSNKTIRGLYG
                     SLKGQYIGLELTGGEMVRKTIAGGDPDTYTAFRQQALGGKLSLGTERGLLMSLTLARN
                     RDVINSLNPEDYISYDPETADTLFVVAPQDNLVASFDTRINIPALNTNLGVEVAGSLY
                     NRNTWPGPISAEEISQYLNGFDYIDPADLAEVFVINRNMEPILPGLPNCAARAWLRTN
                     FLNNIISVDATATGPVYHALSTWGQKPDTKTVTFSDMFNIGRSFYLSGVYIWQQDNLF
                     GTSASTNTNRIWHLQGTLRLLKYFSLRGSYFNSDATNDENTAVGEGLVFMPCLRNSHY
                     YTVGAGFNNPRVDYLPYFCELNYRNGTDFSNTIQFGEYRNSTNSINFSLLSKFGRIPL
                     RLQVNASLATLANDLSEYIPVLSFNYISYHARISYSLFKDKLIPWLQYRRSDYNIANK
                     GDKCNDFLSLGLEAFPWKNMSVNTSLNRMTNVDKSNSNLKHQTITWSLTLGQRF"
     gene            complement(2525..3199)
                     /locus_tag="PLO35_08995"
     CDS             complement(2525..3199)
                     /locus_tag="PLO35_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79846.1"
                     /translation="MNQKHIFIALALLSTSLLSAAGIAVVSGNQGKCILRRANKSIKY
                     TNGTILESNDVLETEKKSGLALKFVDNLTSMHLFSSTQISIDSKAIISGLSKQVELNK
                     GSLYVKQLPGSGTLNVAFDQSKVSSDAASFLIRTEGGGKAWISVFSGTVTFTNKNGAI
                     TTLEAGRSAEMQPRGQVKANPAKDSELTAEELAVVKPTPEHDKRRITVPMANNQGQIK
                     YVEFAW"
     gene            complement(3202..>4425)
                     /locus_tag="PLO35_09000"
     CDS             complement(3202..>4425)
                     /locus_tag="PLO35_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79847.1"
                     /translation="PDGSQGRGDQLCGPGGQDCSPSFKLNEQRGIMRKHFFLSMVLLT
                     VLVVLGAQNPDVNPWFTPNSINVNSFKTASLDPTAPWNQPILTNLVISNHSDAAFRFY
                     LKLDIYWSGISDPLASIIYISKETIPALGQFYPLTNQDLVTNQPSTLFDRLGNNAFSL
                     DDITNRSSVLRQAALAGYFPDGVLSLIVKVQPESSGSQDWNGASEATFTIWIVNAEAI
                     SLISPGTPASSSPPVVDQTPVNFVWNSIDTNINVYKLTIREYPNNLPPDINSLADTGS
                     LFYDQHVYGNQFTEFLPFNQGSYYAWRISTKRWTELDSYVSPGPWREDDPGLISSGWN
                     IFRYSLFESGADPIHRICAVLNSLGEDKLEALFNEGFRPTGEIIWNGRSYSGQSAIDL
                     IEQLLGQRAVITVWE"
BASE COUNT         1009 a         1069 c         1178 g         1169 t
ORIGIN      
        1 gatgtttccc tgtgttgcct cccgccaaat gcccgcataa aatgcgggaa tcatacgggt
       61 ggcatgctag atgggaacta gcatgggtta aggtaggaca ggcactttgt gaaaatcatt
      121 gcctgatttg atcagaatct ctgtccaagc gtgaggctcc aggtgatggt ctggtgttta
      181 agattactgt tggatttatc cacattcgtc attcggttca aactggtgtt cacgctcatg
      241 tttttccagg gaaatgcctc caggcccagg ctcagaaagt cattgcactt gtctcctttg
      301 tttgcgatat tgtaatccga acggcgatac tgcagccagg gaatcagctt gtccttaaag
      361 agtgaatagc tgatcctggc gtgatagcta atgtagttga agcttagcac cgggatgtat
      421 tccgatagat cgtttgccag tgtagccagc gaagcgttga cctgcagacg cagcgggatc
      481 ctgccaaact tgctcaagag gctgaagttg atgctgttag tgctgttccg gtattcaccg
      541 aattggatgg tattgctgaa gtccgtccca ttgcgataat tgagttcgca gaaataggga
      601 aggtagtcga cccgcggatt gttgaaaccc gctcccactg tatagtaatg ggagttgcga
      661 agacatggca tgaaaaccaa gccttccccg acggctgtgt tttcgtcatt ggtggcgtcg
      721 ctgttgaaat aagagccgcg caggctgaag tatttcagca acctcagcgt tccctgcaga
      781 tgccagattc ggtttgtatt ggtgctggcc gaagtgccga agaggttgtc ctgctgccag
      841 atatataccc cggaaaggta gaatgagcgg ccaatgttga acatatcgga aaacgtcacc
      901 gtcttagtgt caggtttctg tccccaggtg ctaagggcat ggtaaactgg gccggtggca
      961 gttgcatcca cggagatgat gttattgagg aaattggttc gcagccaagc cctagccgcg
     1021 caattcggca gtccgggcag gatgggttcc atgttgcggt tgatcacgaa aacctcggcc
     1081 agatcagcgg gatcgatata atcgaaacca ttcaaatact ggctgatctc ctcagcggag
     1141 atggggcctg gccaagtgtt gcggttgtag aggctgccgg ctacttccac tcccaggttt
     1201 gtgttcaagg ccgggatgtt gatgcgggtg tcgaaactgg ccaccagatt gtcctgaggt
     1261 gccaccacaa agagagtgtc ggcggtttcg ggatcgtagc taatgtaatc ctcgggattg
     1321 agagagttga tcacatcgcg gttgcgggcc agagtaaggg acatcagaag tccccgctcg
     1381 gtaccgaggc tgagtttccc tcccaaagcc tgctgccgga aagccgtata ggtgtctgga
     1441 tcgcctccag caatggtttt gcgcaccatt tctccacctg tgagttccag tccgatatat
     1501 tgtcccttca aactaccata gagaccgcgg atggttttat tgctcatagc cagaggacta
     1561 agctgcggaa tttggtcgcc agcctgaatg gttaaaaagc ccagttgcag ccccgcgcag
     1621 tagcggtcta tgggctggcg gttagggttc tccaagctgg agtggagggc gttcaagttc
     1681 agcttaagct gcttgtaacg gatgttaaaa tccagccagc cggcgtagct gttttttgga
     1741 tcttcaggct ggttgggctc atactcttct tgaggccggt agcgatggaa gttggatgcg
     1801 aaattaacac cgccccaggt atagatcttg attctgccac ccttgggatc gaccagggta
     1861 gtaaaaacct gtgatttaat cagctttccc tggcggtcga gcgcggtgac tacagcttcg
     1921 taattcttag tcttgggttt gggatcgcgg taactgagca gattctcagt tatcacagcc
     1981 aaagaagtca cgttgcggcc attcacccat acctctatgg tggctggatc gatttggtca
     2041 gcgatggcaa aataggatac caccagcacg tattcaccct tctgggcaaa atcctcctct
     2101 gtgctcagta gcacgaaacc aacatcttcc ttaccactct ggatctctgt ttttagcgta
     2161 taaggttcgc cgcccaacgc ttcatccgga taagagaaag tcgagccgtc tgtgagttcg
     2221 aaatcgaacc agtattgcag ctcacggtct tgtagttctt ggggctgcag ccgtactgta
     2281 aatgcttggg ttccgggcag cggcgagtcc atggtcttct ctgaatagcc cgtctctccc
     2341 cttggaccgt agcagagatt gattttggcg atagtttcta cctctgctgg tatttctatg
     2401 cttaaatcaa cgctggagcc ggatacatag gtccggggcg gctcatgaat tgctacgata
     2461 cctggcagaa aggatgtcaa ggcgatcaga aagagaatta atgatgtctt tttcatattg
     2521 gggctcacca cgcaaattct acatatttga tctgaccctg gttgttggcc atgggtaccg
     2581 tgatcctgcg tttatcgtgt tccggagtgg gtttcaccac ggccagctct tctgctgtca
     2641 gttcagaatc ttttgcagga ttcgctttaa cctggcccct tggttgcatt tccgcgctgc
     2701 gtccagcttc cagagttgtg atcgcaccat tcttattggt gaaagtcacc gttccggaga
     2761 acactgaaat ccaggctttc cctccacctt cggttctaat caggaacgaa gcagcgtctg
     2821 aactcacttt gctctggtca aaagcaacgt tcagggtgcc gcttccgggc agttgcttaa
     2881 cataaaggct tcctttgttc agctccacct gtttgctcaa accagatatt atggctttgg
     2941 agtcgatgct gatttgggtg gaggaaaaca ggtgcatact ggtcaggtta tcaacgaact
     3001 tcaacgccag cccggatttc ttttcggtct caagcacatc gttgctttcc agaattgttc
     3061 cattggtata tttgatgctc ttgttcgccc ggcgcaggat gcatttgccc tggttgccag
     3121 agaccacggc tattccagcg gcggatagca aacttgtgct caaaagggct aatgcgatga
     3181 agatatgttt ctgattcatc ttcactccca taccgtgatc acggctcttt ggccaagcag
     3241 ttgctcgatc aagtcgatgg cactttggcc gctgtagctg cggccgttcc agatgatctc
     3301 gcccgtgggc cggaaccctt cattgaagag ggcttccagc ttgtcttccc caagcgagtt
     3361 cagcacagcg cagatcctgt gtataggatc tgccccactc tcgaacaagc tgtatcggaa
     3421 gatgttccag ccgctgctga tcaagccggg gtcatcttcg cgccagggac ctggagaaac
     3481 ataggagtcc aactccgtcc agcgtttggt actgatccgc caagcataat agcttccctg
     3541 attgaagggc agaaactcgg tgaattgatt gccatacaca tgctgatcat aaaacagcga
     3601 tcctgtatcc gcaaggctgt taatgtccgg aggcagatta ttcggatact cccggatcgt
     3661 gagtttgtat acgttgatgt ttgtatcgat gctgttccag acaaaattca ctggagtctg
     3721 gtccaccact ggtgggcttg aagaagccgg cgttccagga ctgatcagtg aaatagcttc
     3781 tgcgttgacg atccagattg tgaatgtagc ctcgctcgct ccattccagt cctgggaacc
     3841 gctgctttcc ggctgcactt tcactatcag gctaagcact ccgtccggga aataacctgc
     3901 caatgcggct tggcgcagca ctgagctgcg gttggttata tcatccaatg aaaaagcgtt
     3961 attacccaac cggtcgaaca gggtggaggg ctgatttgtg accaaatctt ggttcgtgag
     4021 aggatagaat tggcctagcg cagggattgt ttctttagaa atgtatatga tcgatgccaa
     4081 cggatcgctg atcccgctcc agtaaatgtc cagtttcaaa tagaaacgaa aagcggcatc
     4141 agaatgattg ctgataacca agttggtcag tataggttgg ttccaaggag cggtgggatc
     4201 taaactagcc gttttgaatg aattgacgtt gatcgagttg ggcgtgaacc agggattgac
     4261 atccggattt tgggccccaa gcacgaccaa cacagtcaaa agtaccattg atagaaaaaa
     4321 atgttttctc atgatgcctc tttgttcgtt taatttaaag ctaggcgaac aatcttgtcc
     4381 accaggtcca caaagctgat ccccacggcc ttggctgcca tcggg
//
LOCUS       DAOSFN010000174         4421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_17829_length_4421_cov_8.786532, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000174 DAOSFN010000000
VERSION     DAOSFN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17829_length_4421_cov_8.786532"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLO35_09005"
     CDS             complement(<1..644)
                     /locus_tag="PLO35_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH79848.1"
                     /translation="MKYCINCGKELSDKAKFCAHCGERQSLPETPDPIAQTKPKKPEH
                     SKAQLKGGSASFPAVNLNMLDPGTDFNGYHIIRVMNKDEEGIKYIASKDERQYVLKLF
                     FHSRLTNLDKLFGLQMRLSRLNLLDDEHIARVEEVNQTSEPAYMAARFVDGISLDRLK
                     KQHPEQVTEDLVRDVAVQLMQTAISIRQQGLTITKLNLSGLMIDKAGKLVILSSG"
     gene            complement(675..3818)
                     /gene="ileS"
                     /locus_tag="PLO35_09010"
     CDS             complement(675..3818)
                     /gene="ileS"
                     /locus_tag="PLO35_09010"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOH79849.1"
                     /translation="MYKTIDLKESARALEARVRDYWHQHSLAKKSEDFREGSPRFVFF
                     EGPPTANGKPGIHHVMARTLKDAVCRYKTMTGYQVKRKAGWDTHGLPVEIEVEKQLGL
                     EDKRAVEEYGLEKFCAHCRDSVFSYEKLWREMTELMGYWIDLDHPYVTLTNDYIESVW
                     WILNNLFERDLIYKAHRIVPYCPSCGTPLSSHEVAQGYKDVEDPSVFVRFKARDEDNT
                     WYLAWTTTPWTLISNVALAVHPDETYVKVLFQEQQLILAKARLEVLEGDYQVLEEFAG
                     SALERRPYEPLFTFIGVDKPAWYVGLADYVSMSDGTGIVHTAPAFGQDDYALGRKYDL
                     PFIQPVDGAGKFIAAIAPWAGLFVKDADKDIIRHLKESGALFRRAQVRHSYPHCWRCD
                     NLLIYYARDSWYIRTTQFKEQLLENNRAIKWYPSFVGEKRFGEWLENNVDWALSRDRF
                     WGTPLNIWVCEDCGHKHSIGSIEALKQQGRLMDGSAVPENIELHKPYIDDVLLACDKC
                     QGVMRRTPEVIDCWFDSGSMPFAQWHWPFENADRFDTELFPADFISEGIDQTRGWFYS
                     MLTISTLIKGVSSYKSCLVNDLILDKNGQKMSKSKGNSVDPIQIMHDQGADAVRWYLL
                     EVSPPWVPTRFDVEGVREINSKFIGTLKNVYSFYATYANIDGYSAVIENLSGSPENWH
                     REAEIDRWIISRFHSLIGKVRAWMEVYEFTKTVRAIQDFVIDELSNWYVRRSRRRFWA
                     MELTADKVEAYRTLHQMLLGVAKLIAPFAPYLAEEIYLNLGAGESVHLSDYPEADPKF
                     IDEALEAEMRLVIDIVQLGRAARNACQIKIRQPLGTMHVPARAKATVERMFSLIQEEV
                     NVREINFVEEDDDFVHYELKPDFKVMGPKYGKLMKSIGSALQKVKGHDVLASFAREES
                     YPLKVEDQRVELVSEDVVVHISPREGYVFESVRDLFVALDITMTPELRLEGYSRELIN
                     KIQYTRKEQGFEIMDRVTVTLSGDAEIQKAMEAHGEYIRSETLCDRLEWQPDLILGQE
                     LDLNGKSVRMLVSKVTQ"
     gene            complement(3890..4249)
                     /locus_tag="PLO35_09015"
     CDS             complement(3890..4249)
                     /locus_tag="PLO35_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79850.1"
                     /translation="MFKLDDTHLEAAQAIAVRNRVKKSCDNCYDRGWIGVSEQNLLVL
                     CTRCVDMEKAIEEWKTYVNDHEDLKEHFAELFEEKEVEEEVGHALPGKPEHKKDHPHA
                     QQNFLPGQKRMGRAKKI"
BASE COUNT          902 a         1205 c         1203 g         1111 t
ORIGIN      
        1 ccgctggata ggattaccaa cttgccagct ttgtcgatca tcaggccgga gagattgagc
       61 ttggtgatgg tgaggccctg ctggcggatc gagatggccg tctgcatcaa ctgcaccgcc
      121 acgtcgcgca ccaaatcctc ggtcacctgt tccgggtgct gctttttgag cctgtccagc
      181 gaaatcccgt ccacaaagcg ggccgccatg taggcaggct cactggtctg gtttacttcc
      241 tcaaccctgg cgatatgctc atcgtccagc agattgagcc ggctgagccg catctggagg
      301 ccaaagagct tgtccagatt ggtgagccgg gaatggaaaa agagcttgag cacatactgc
      361 cgctcatctt tggaggcgat gtatttgatg ccttcttcgt ctttattcat cacccggatg
      421 atgtgatagc cgttgaaatc ggtgcccgga tcaagcatat tcagattgac ggcgggaaac
      481 gaggcagaac cacctttgag ctgtgctttg gagtgctccg gctttttggg cttggtctga
      541 gcaatcgggt ccggggtttc tggaaggctt tgccgctcac cgcagtgggc acaaaacttg
      601 gccttgtcgc tgagctcttt gccgcagttg atgcaatact tcataccttc aggcctccgt
      661 ttcgggcagg gccatcactg tgtcaccttg cttaccagca tgcgcacgga ttttccgttg
      721 aggtccagtt cctgtcccag gataaggtca ggttgccact ccagccggtc gcagagagtt
      781 tcggagcgga tatactctcc atgggcctcc atagctttct ggatttccgc gtctccgctt
      841 agggtcaccg tcacccggtc catgatctca aacccctgtt ccttgcgggt gtactggatt
      901 ttgttgatca gttcgcgcga gtatccttcc aaccgcagtt caggcgtcat ggtgatatcc
      961 agggccacga aaagatcgcg aacgctttca aacacgtatc cctcccgggg agagatatgc
     1021 accaccacgt cctcggaaac cagttccacg cgttgatctt ccaccttgag gggatagctt
     1081 tcctctcggg caaaggaggc caacacatca tggcccttca ccttctggag cgcggacccg
     1141 atggatttca tcagtttacc gtatttggga cccatgacct tgaaatcagg cttgagctca
     1201 taatggacaa agtcgtcgtc ttcttccaca aagttgatct cgcgcacgtt gacctcttcc
     1261 tggatgagag agaacatccg ctctaccgta gctttggcgc gggcgggaac atgcatggtg
     1321 cccagaggct gacggatttt gatctgacaa gcgttgcggg ccgcccggcc aagctggacg
     1381 atgtcgatca cgaggcgcat ttctgcttcc agggcttcgt ctatgaactt gggatcggcc
     1441 tcaggataat cgctcagatg cacactttcg ccagctccca gattgaggta gatctcctct
     1501 gccagatagg gagcgaaggg agcgatcagc ttggccacac cgagcagcat ctgatggagg
     1561 gtgcggtagg cttccacctt gtccgcggtg agttccatag cccaaaaacg acgtcggctg
     1621 cgtcgcacat accagtttga aagctcgtcg atcacaaaat cctggatggc gcgcacagtt
     1681 ttggtaaact cataaacttc catccacgct cgcaccttgc caatcaagct gtgaaagcgt
     1741 gagatgatcc agcggtcgat ctcggcttcc cgatgccaat tctccgggga tccgctaagg
     1801 ttctcaatta ccgcgctata gccatcgata ttggcatagg tggcataaaa ggaatacacg
     1861 ttcttgagcg tcccgataaa cttggaattg atttcccgca ctccctccac gtcaaaccgg
     1921 gtgggcaccc agggaggact gacttccagg agataccagc gcaccgcgtc ggctccctga
     1981 tcgtgcatga tctggatggg atcgacgctg ttgcctttgg atttgctcat cttctggccg
     2041 ttcttatcca aaatcaaatc gttgaccaga cagcttttat agcttgaaac acccttgatg
     2101 agagtggaga tggtgagcat ggagtaaaac cagccgcgag tctgatcaat tccttccgag
     2161 atgaaatcgg cggggaagag ctcggtgtca aagcgatcgg cattttcaaa aggccagtgc
     2221 cactgcgcga agggcatgga gccgctgtca aaccagcagt ctatcacttc cggagtgcgg
     2281 cgcatcacac cctgacattt gtcgcaagca agcagcacgt catcgatgta gggtttgtgg
     2341 agctcaatgt tttccggcac ggcagagcca tccataagac gtccctgctg ttttaaagct
     2401 tcgatggatc caatggagtg cttgtgaccg caatcctcac agacccagat gtttagaggt
     2461 gttccccaaa aacggtcccg ggaaagggcc cagtccacgt tgttctcgag ccattcgcca
     2521 aagcgcttct ctcccacgaa ggaggggtac cacttgatgg cgcggttgtt ttccaacagc
     2581 tgctctttaa attgggtggt gcggatatac cagctatccc gcgcgtaata gatgagcagg
     2641 ttgtcacagc gccagcagtg gggatagctg tgacgcacct gagcgcgtct gaacagggct
     2701 ccgctttctt tgaggtgtct gatgatgtct ttatcggcgt ctttgacaaa gagtccggcc
     2761 cagggagcga tggcggcgat gaattttccg gccccgtcca caggctggat gaagggcagg
     2821 tcgtacttgc gccccagggc atagtcatcc tgtccaaaag ctggagctgt gtgcacgatc
     2881 ccggtaccgt cgctcatgct gacataatct gccaggccga cataccaggc gggcttatcg
     2941 accccgataa aagtgaagag cggctcatag ggccttcttt ccagcgcgga accggcaaac
     3001 tcctccagga cctgataatc gccttccagc acttccagac gggctttggc caggatgagt
     3061 tgctgttcct gaaagagtac cttcacataa gtttcatccg gatgcacggc cagggcgacg
     3121 tttgagatca gggtccaggg cgtggtagtc caggccagat accaggtgtt gtcctcatcg
     3181 cgggctttga aacgcacaaa tacggatggg tcttccacgt ccttatatcc ctgcgccact
     3241 tcatgcgagg agagcggcgt gccacagctg gggcagtagg gcacgatgcg atgggctttg
     3301 tagatgaggt cgcgttcaaa gagattgttg aggatccacc agacgctctc gatgtagtcg
     3361 ttggtgagcg tgacgtaagg atggtccaga tcgatccagt agcccatcag ttcggtcatt
     3421 tcccgccaca gcttttcata gctgaacacg gaatcgcggc agtgggcgca gaatttctcc
     3481 aggccatact cttccacggc gcgtttatct tccagaccga gctgcttttc cacctcgatc
     3541 tccacgggca ggccatgcgt gtcccaaccg gctttacgct tcacctgata gccagtcatg
     3601 gtcttgtatc tgcagaccgc gtccttgagg gtgcgggcca tcacgtgatg gatgccgggt
     3661 ttgccattgg cggtgggggg tccttcaaaa aatacgaatc tgggactgcc ttcccggaag
     3721 tcctcactct ttttggccag cgagtgttgg tgccagtaat cgcgcaccct ggcttccaga
     3781 gcgcgggcgc tttccttgag gtcgattgtt ttgtacatat atcctatggt ttccgtctga
     3841 tctgtttcag gatccttttt ggcgggatca ggcctggctg ggtatcagcc tagatttttt
     3901 tggcccggcc catgcgtttc tggcccggca ggaaattctg ttgggcgtgc ggatggtcct
     3961 ttttgtgttc tggtttgccc ggcagagcgt gccccacttc ctcttccact tccttttctt
     4021 caaagagctc cgcgaaatgc tctttcaggt cttcgtggtc gttcacatag gttttccact
     4081 cttcgatggc tttttccata tcgacgcagc gggtgcagag taccagcaga ttctgctcgg
     4141 atacgccgat ccagccacgg tcatagcagt tatcgcagct cttctttaca cgattacgga
     4201 ccgcgattgc ctgagcggcc tccaggtgcg tatcatcgag tttaaacatt gtgtgctcct
     4261 tgtttatggt ttgtatcagg ttttgggttt cgctaaggga atctttcact aacgtaccat
     4321 tatttccaga gggcttttct ttacaagctt ttttttagcc ccttgcttcc cgaagagcat
     4381 tacggactca ttacggactt agtccgtaat gagtccgtaa t
//
LOCUS       DAOSFN010000175         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_18247_length_4344_cov_7.685008, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000175 DAOSFN010000000
VERSION     DAOSFN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18247_length_4344_cov_7.685008"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /gene="dnaX"
                     /locus_tag="PLO35_09020"
     CDS             complement(<1..1332)
                     /gene="dnaX"
                     /locus_tag="PLO35_09020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOH79851.1"
                     /translation="MSYIVLARKYRPQTFSEVYAQDHVTEILQNAIESKRIAHAFLFT
                     GPRGVGKTSMARILAKSLNCEEGPSAHPCNKCHNCLEITAGTSSDVIEIDGASNTGVD
                     DIRELQKELMYAPSQSQYKIYIIDEVHMLSKNAFNALLKTLEEPPENVLFIFATTEAQ
                     KVLATIVSRCQRYDFKRIPVEAIVRRLREITASENIQIDDESLYMIARKADGGMRDAL
                     SLMDQVLSYCLNDVSGEKVREIFGMIPNQVFSNIFSMVKRRDSANLIASLHQVLEDGM
                     DPQELLINMTEFIRVTLLRKTGVTPQDIVADEYPLFDAVADEFTPTELLYLISTLMQT
                     RTDLKGSSNPQLVLEVLMLKLSRMQEMNEISELIKLVRSGNLLPAARPTSGAADPAPA
                     TSPAPRDAKPAPQYSPGSTPVTHHEAPMEKISFDLDSVQAHREKIDVRIKRI"
     gene            1530..2753
                     /locus_tag="PLO35_09025"
     CDS             1530..2753
                     /locus_tag="PLO35_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOH79852.1"
                     /translation="MKLEGKVVIAQGGGPTAVINQSLVGAALEARKFSQVTRVYGALY
                     GVQGIVNEDFVDLTQETTHNLEQVANTPSSALLSTRDKPDEAYCKDIFKVLKAHDVRY
                     FFYIGGNDSSDTVRIVNEQARQADYEFRAIHIPKTIDNDLVLNDHTPGYGSAARFVAS
                     AFGGVNLDNRALPGVYIGVVMGRHAGFLTAASALAQKYPDDGPHLIYLPERPFHRDKF
                     LKDVHRVYNQFGRCVVAVSEGIVDEKGVPMMTKLTNSSETDAHGNVQLSGTGMLGDLL
                     SDLVKTKLHIKRVRSDTFGYLQRSFLGCVSDVDQREAREVGERAAHYAIWYNLDGSIS
                     IQRTGFYSVNYQLEDLANIARKTRHMPDEYINPEGNGVTDEFRYYLRPLLGSGFPMAH
                     RLRAPRVGKLLDGNA"
     gene            2815..4152
                     /gene="gdhA"
                     /locus_tag="PLO35_09030"
     CDS             2815..4152
                     /gene="gdhA"
                     /locus_tag="PLO35_09030"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOH79853.1"
                     /translation="MTFQEFMAKVAARNPGEPEFLQAVKEVVETIWDAYESNPRFVKA
                     NILERIVEPERVIMFRVPWMDDKGEVHVNRGFRVQFNSAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTTLPMGGGKGGSDFDAKGKSDAEIMRFCQSFMAELFRHVGPDTDV
                     PAGDIGVGGREIGYLFGYYKKLANEFTGVLTGKGRTWGGSLIRPEATGFGVVYYAEEM
                     LKTQGKTFDGMKVALSGFGNVAWGAAQKINELGGKVVTLSGPDGYIYDEDGVSGDKID
                     YMLELRASNNDRVIDYADVYPNAKFIPGKRPWEVPVDVAIPCATQNELDAEDARTLVK
                     NSCFCVVEGANMPSTPEAVEIFQSAKILFAPGKAANAGGVATSGLEMTQNSMRLNWNR
                     EEVDERLHSIMKNIHEACREHGTQADGYVNYVKGANIAGFLKVANAMCDQGLI"
BASE COUNT          974 a         1225 c         1168 g          977 t
ORIGIN      
        1 gatgcgtttg atcctgacat cgatcttctc acggtgggcc tgcacactat ccagatcaaa
       61 actgatcttt tccatcggcg cttcgtgatg cgtcacaggg gtggagcctg ggctatattg
      121 cggggcgggt ttggcgtccc gcggtgccgg cgatgtggct ggagcgggat ctgccgctcc
      181 cgaagtaggt ctggcggccg gcaggaggtt tccactccga accagcttga tcagctcgga
      241 aatctcgttc atctcctgca tgcggctcag cttgagcatc agaacctcca gcacgagctg
      301 gggattggaa ctgcctttta gatccgtcct ggtctgcatc agggtgctga tcagatagag
      361 cagctccgta ggcgtaaatt catccgctac ggcatcaaag aggggatatt catcggcgac
      421 gatgtcctgg ggcgtcacac cagttttgcg cagcagcgtt actctgataa actcagtcat
      481 gttgatcagc agttcctgcg ggtccatgcc gtcttccagc acctgatgca gagacgcgat
      541 cagattggca gagtcgcgcc gtttgaccat actgaagatg ttggaaaaca cctgattggg
      601 gatcattcca aaaatctcgc gcactttctc cccgctgacg tcgttgaggc aataggagag
      661 cacctggtcc atcagcgaaa gcgcgtcccg catcccgcca tcggcttttc gggcgatcat
      721 gtagagggat tcgtcgtcga tctgaatgtt ttcgctggcc gtgatctctc tgaggcgcct
      781 tacgatggcc tccaccggga tgcgcttgaa atcataacgc tggcagcgtg agacgatggt
      841 ggccagcacc ttttgcgctt cggtggtggc aaagataaaa agcacgtttt ccggaggctc
      901 ttcaagggtc ttgagcagcg cgttgaaggc attcttggag agcatgtgca cttcatctat
      961 gatgtatatc ttgtattgcg actggctggg agcgtacatc agctccttct ggagttccct
     1021 gatatcgtcc acgcccgtgt tggaagcgcc gtcgatctct atcacgtcag aagaggtgcc
     1081 ggccgtgatc tccagacagt tgtgacattt gttgcaggga tgagcgctgg gcccctcctc
     1141 gcagttaagg ctctttgcca gaatcctggc catcgaggtc ttccccacac cgcgcggtcc
     1201 tgtaaacagg aaggcgtgcg cgatccgctt gctctcgatc gcgttttgaa gaatctccgt
     1261 cacgtgatcc tgggcgtaga cctcagagaa ggtttgaggc cggtattttc tggctaatac
     1321 gatgtaactc atcttacctc caaccccaat cttttgggcc ttgttttttt agcaagcatt
     1381 ttctgctgga caagaatggc gcgtcttata attgagaatt ggtgagcgcc cgcggctgat
     1441 gaacacgaaa tacccgcttg atcaggttgc ggactactca tgacgcttta ctgctaacca
     1501 atgattatat agattcacgg gaggaatcaa tgaaactgga aggaaaagtg gtgatcgccc
     1561 agggtggcgg tcctaccgcg gtgatcaatc aatccctggt gggagcggcc ctggaagccc
     1621 gcaagttcag ccaggttacc agagtttacg gcgccctcta cggcgtccag ggcattgtca
     1681 atgaagactt tgtggatctc actcaggaga ccacccacaa cctcgagcaa gtggccaaca
     1741 ccccctcttc ggcactgctc tccactcgcg acaaaccgga tgaagcctat tgcaaagata
     1801 tcttcaaggt gctcaaggcc cacgacgtcc gctatttctt ttatattggc ggcaacgact
     1861 cttccgatac cgtccgcatc gtcaatgaac aagcccgcca ggcggattac gaatttcgcg
     1921 ccattcacat ccccaagacc atcgacaacg atctggtgct caacgaccac actcccggct
     1981 acggatcggc cgcccgtttt gtggcctccg cctttggagg tgtaaatctg gacaaccgtg
     2041 ctctgccggg tgtgtatatc ggcgtcgtga tgggccgtca cgccggattt ctcaccgccg
     2101 ccagcgctct ggcccaaaag tatcctgacg acgggcccca tctcatctac ctgccggagc
     2161 ggccattcca tcgggataag tttctgaagg atgtccaccg ggtttacaat caattcggac
     2221 gctgcgtggt tgccgtttcc gaggggattg tggatgaaaa aggcgtcccc atgatgacca
     2281 agctcaccaa ctccagcgag accgacgccc atggcaatgt ccagctttcc ggcaccggta
     2341 tgctcggcga tctgctcagc gatctggtga agaccaagct ccatatcaaa cgggtgcgca
     2401 gcgacacctt cggctatctg cagcgatcat ttttaggctg cgtttcggat gtggaccaac
     2461 gggaagcccg cgaggtaggc gaacgcgccg cccattatgc catctggtac aatctggacg
     2521 gctcgatctc tatccagcgc actggtttct actccgtaaa ctaccagctg gaagacctgg
     2581 ccaacatcgc ccgcaagacc cgtcacatgc ctgatgaata catcaatccg gaagggaacg
     2641 gcgtcacgga tgaattcaga tactatctgc gccccctcct gggctccggt tttccgatgg
     2701 cccaccgcct cagagctccc agggtcggca aacttctgga tggaaatgct tgacattttc
     2761 tctgctttat aaaacgtggc taataaataa caaaaataca catggaggat taccatgacc
     2821 tttcaagaat tcatggccaa agtagccgcc agaaacccag gtgaaccgga gtttctgcaa
     2881 gccgtcaagg aagttgtcga gaccatttgg gatgcctatg agagcaatcc ccgctttgta
     2941 aaagccaata ttctcgagcg tatcgtggaa ccagagcgcg tcatcatgtt tcgcgtcccc
     3001 tggatggacg acaaaggcga agtgcatgtg aaccgcggat tccgcgttca gttcaacagc
     3061 gctatcggcc cctacaaggg cggcctgcgt ttccacccca gcgtcaacct ttccattttg
     3121 aagttcctcg gctttgagca gatcttcaaa aacagcctca ccaccctccc tatgggcggc
     3181 ggtaaaggcg gtagcgattt tgacgccaag ggcaaatccg acgctgagat catgcgtttt
     3241 tgccagagct ttatggctga actcttccgc catgtgggtc ctgacacgga cgttcccgcc
     3301 ggcgatatcg gtgtgggcgg acgcgagatc ggctatctct tcggctacta caaaaaactg
     3361 gccaacgaat tcaccggcgt tctcaccggt aaaggccgca cctggggcgg cagtctcatc
     3421 cgtccggaag ccaccggctt tggcgtggtt tattatgccg aggaaatgct caagacccag
     3481 ggcaagacct ttgatggcat gaaagtagcc ctctccggct tcggcaacgt agcctggggc
     3541 gccgctcaaa agatcaacga actgggcggc aaggtcgtca ccctctccgg tcccgatgga
     3601 tacatctatg atgaggatgg cgtctccgga gacaagatcg actatatgct cgagcttcgc
     3661 gcttccaaca acgaccgcgt tatcgactat gcggacgtct atcccaacgc caaattcatc
     3721 cccggaaaac gcccctggga agttcccgtt gacgttgcca tcccctgcgc cactcaaaac
     3781 gagctggacg ccgaagatgc caggactctc gtgaaaaaca gctgcttctg cgtggttgaa
     3841 ggcgccaaca tgcccagcac tccggaagcc gttgagattt tccagagcgc caagatcctc
     3901 ttcgctcccg gaaaagccgc caatgccggc ggagtcgcta ccagcggtct ggaaatgacc
     3961 caaaacagca tgcgcctcaa ctggaaccgc gaagaagtgg acgaacgcct tcacagcatc
     4021 atgaaaaaca tccacgaagc ctgccgcgaa cacggcaccc aagccgacgg atatgtcaac
     4081 tacgtcaagg gcgccaacat tgccggcttc ctgaaagtag ccaacgccat gtgcgatcag
     4141 ggtctcatct aagagcatac cctgcaccat atatcagacc ccggagcgat ccggggtttt
     4201 tttttggggg agtgtaacaa gggggccagg tgattgacag agggcggaat tgatgaattg
     4261 atggcaactg atgagaggtt ttaaggtttt agggttttac tgcagagata cagaggtttc
     4321 ttttttcact acagagctac ggag
//
LOCUS       DAOSFN010000176         4309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_18452_length_4309_cov_8.565115, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000176 DAOSFN010000000
VERSION     DAOSFN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18452_length_4309_cov_8.565115"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLO35_09035"
     CDS             complement(<1..460)
                     /locus_tag="PLO35_09035"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOH79854.1"
                     /translation="MFSYTLQARSGKARAGIIETDHGKIITPVFMPVGTLGTVKAMSP
                     HELEESHAQIILGNTYHLYLRPGHELIREAGGLHKFISWSKPMLTDSGGFQVMSLAAL
                     RKITRDGVQFQSHIDGSRHLFTPESVIAIQEALGADIIMSFDECPPYPATR"
     gene            complement(467..1753)
                     /gene="rseP"
                     /locus_tag="PLO35_09040"
     CDS             complement(467..1753)
                     /gene="rseP"
                     /locus_tag="PLO35_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOH79855.1"
                     /translation="MTLLFTLLALALMIFIHELGHYWTARRFGVGIEKFSIGFGAPLL
                     KFHRKGVLWQIGWIPLGGYVKMQGENPDDTDADPALSFQQKPWWQRALIAFSGPFANL
                     LLGLLLFTVSFLLPIKVEDHHPVVFEADGKWARVFERGDSLTAVNGKPVKGWMEALSE
                     LSASKENSIALIRADSLTSLKVPAVEVDSLLRSLSPVLPTEIGEVYAGMPAWRAGIRS
                     GDVVVAVDSVAVYDWYAMREKIVGSPRSSVRLTLIRDGKLLHRDVVLEANAALGQERL
                     IGITNSQPVKSTRRFNLWQSISYGVPSGVEFVGRNYSSIFKLFAKPEQLARNLGGPVM
                     IATMSSQVGDKGLSYLILFFGSISLIFMTMNLLPIPILDGGHILFCVIEGITGKPVPL
                     RVQGILQRIGLTLLLMLMILATYSDLSKVLSRALSG"
     gene            complement(1750..>2469)
                     /locus_tag="PLO35_09045"
     CDS             complement(1750..>2469)
                     /locus_tag="PLO35_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOH79856.1"
                     /translation="DTSGDGKLQLSFSLPVSADAHGKEAARLLLRQIGLDDIEITGMR
                     NLNEGFTHFIAYAVTSAGVDPDSIKVKVVETPVMDMEATDAYIKERIGRELTVVGACI
                     ESDAHTVGIDAIMNMKGYNHRYGLERYQGFNAVNMGAQVLSEDLLAKARELKADAILV
                     SQVVTQKNIHIKNLTRLIELAEAERLRDKMLFICGGPRISHELAIELGYDAGFGTGSY
                     STDVASYIAITMAERLARKAK"
     assembly_gap    2470..2569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2571..4127)
                     /locus_tag="PLO35_09050"
     CDS             complement(2571..4127)
                     /locus_tag="PLO35_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOH79857.1"
                     /translation="MLKLELNDDLVSRARKAARQITGSFGWLFEDYSSLTIERTVLRM
                     MGLDGALKDGKPLPNVVTDQLKAKGALGKGAAIRLANAMLNKDLSATQIAEAMGQHHL
                     DITALPMAPADEIYSLLQELTDTMLGHISSQKARREEMFRRFGPRRVPAIYVIVATGN
                     IHEDVVQAKAAAREGADIIAVIRSTAQSLLDYVPYGATTVGFGGTYATQENFRIMRAA
                     LDEVSEAEGRYISLTNYASGLCMPEIAVMGAIERLDLMLNDAMYGILFRDINPRRTLL
                     DQSFSRMINAYAGIEIQTGEDNYLTTSDAIEKAYTVTASQLLNEQFALLSGVKPEKMG
                     LGHAHEIDPELENSFSYELAHAMLTRELFPQAPVKYMPPTKFASGNIFKTHLMDAMFN
                     FIGQLTGQGVQLLGMMTEAIHTPHLADRSLAIENAQYLFRAVKDLSSSLMLAPDSFIH
                     QRANQVLEEATLFLEKVAAEGLLEAMARGEFADIKRPETGGKGLDGVFRKDEDYYNPF
                     RERMMKELGL"
BASE COUNT          883 a         1171 c         1216 g          939 t
ORIGIN      
        1 cgcgggtagc aggatagggt gggcattcat cgaagctcat gatgatgtcc gcccccaaag
       61 cttcctggat ggcgataacg ctctcggggg taaagagatg cctggatccg tcgatgtggg
      121 attggaactg cacgccgtcc cgggtgatct tgcgcagggc tgccaggctc atcacctgaa
      181 agcctccact gtccgtaagc atgggctttg accagctgat aaacttatgc aggccacccg
      241 cctcgcggat aagctcgtga cctggtctca aatagaggtg gtaggtgttg cccagaatga
      301 tctgcgcgtg gctttcctcc agctcgtgag ggctcatggc cttaacggtg cccaaagtgc
      361 ccaccggcat gaaaaccgga gtgatgatct tgccgtgatc ggtctcgatg attccggccc
      421 tggctttacc actcctggcc tgaagggtgt agctaaacat ccagcctcat cccgacaggg
      481 cgcggctcag caccttggaa agatcagagt aggtggcaag gatcatcagc atcagcagca
      541 gcgtgagccc gatccgttgc aaaatcccct gcactctaag gggtacaggc tttccagtga
      601 tgccttcaat gacgcagaag aggatgtgcc caccatccag gatgggaata ggcaagaggt
      661 tcatagtcat aaagataagg ctgatgctgc cgaaaaagag gatcagatag ctgagacctt
      721 tgtctcccac ctgagagctc atagtggcga tcatgacggg gccgccaagg ttgcgggcca
      781 gctgttccgg cttggcaaac agcttgaaga tgctgctgta gttgcgcccc acaaattcta
      841 ctccggaggg cacaccgtag ctgatagact gccagagatt gaagcgacgg gtgcttttaa
      901 ccggttggga attggtgatg ccgatgaggc gttcctgtcc cagcgcggca ttggcttcca
      961 acaccacgtc ccggtggagc agtttgccat cgcggatcag ggtcagcctc acactggagc
     1021 gtggcgatcc cacgatcttc tcccgcatgg cataccagtc atagacggcc acgctgtcca
     1081 ccgcaaccac cacatcgccg cttctgatcc cagcccgcca ggctggcatg ccggcataga
     1141 cctctccgat ctcggtgggc aggacaggac tcagacttct gagcaggcta tccacttcca
     1201 cagcggggac ttttaaactg gtgaggctat ccgctctgat cagcgcaatg ctgttctctt
     1261 tgctggcgga aagctcgctc agggcttcca tccatccctt gacgggtttg ccgttcacag
     1321 cggtgagact gtctccgcgc tcaaacaccc gggcccattt gccatccgcc tcaaagacca
     1381 ccggatggtg gtcttccacc ttgatgggca agaggaaaga taccgtgaag agcaagaggc
     1441 ccagcagcag gttggcaaaa ggaccgctga aggcgatcag ggcgcgctgc caccagggct
     1501 tttgctgaaa ggacaaggcg gggtccgcgt cggtgtcgtc gggattttca ccctgcatct
     1561 tgacataacc gcccaaaggg atccagccga tctgccagag cactcctttg cggtgaaact
     1621 tgaggagcgg agcgccaaag ccgatggaaa acttttctat ccccactcca aagcgccggg
     1681 ccgtccaata atggcccagc tcgtgaatga agatcatcag cgccagggcc agaagtgtaa
     1741 acagtagggt cacttagcct tcctggccaa ccgctctgcc attgtgatcg ctatatagct
     1801 ggccacatcg gtggagtatg aacccgtgcc aaaaccggca tcatagccca gctcgatggc
     1861 cagttcatga gaaatcctgg gaccgccgca gatgaagagc atcttgtcgc gcaggcgttc
     1921 cgcttcggcc agttcgatca atcgggtgag gtttttgatg tggatattct tctgagtgac
     1981 cacctgtgac accagaatgg cgtccgcttt cagctcgcgc gccttggcca acaggtcttc
     2041 gctgagcacc tgagcgccca tattgacggc attgaagccc tgatagcgtt ccaaaccgta
     2101 gcggtggttg tagcccttca tgttcatgat ggcatcgatg cccaccgtgt gagcgtcgct
     2161 ttcgatgcag gcccccacca cggtgagttc gcgtccgatc cgctctttga tataggcatc
     2221 ggtggcttcc atatccatca cgggggtttc caccaccttc acttttatgg agtccggatc
     2281 gactcccgca ctggtgacgg cataggcgat aaaatgggta aaaccttcat tgaggtttct
     2341 cataccggtg atctcgatat catcgagacc gatctgcctg agcagcagac gggcggcttc
     2401 cttgccatga gcatcggcag atacgggcag gctgaaggaa agctgcagct tgccgtctcc
     2461 ggatgtgtcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tcataatccc
     2581 agctccttca tcatccgctc ccggaaggga ttgtaataat cctcatcctt gcggaacaca
     2641 ccgtccaggc cctttccacc cgtttcagga cgctttatat cggcaaattc gcctcgggcc
     2701 atggcttcca gcagcccttc agcagccact ttctccaaaa agagggtggc ttcttccagc
     2761 acctggttgg cacgttggtg gataaagctg tccggggcca gcatgaggct ggaggagagg
     2821 tctttgacgg cccggaagag gtattgggcg ttttcgatgg ccagcgagcg atccgccaga
     2881 tggggagtgt ggatggcttc cgtcatcatg ccaagcagtt gcacgccctg gcctgtgagc
     2941 tgaccgatga agttgaacat ggcgtccata aggtgggtct tgaagatatt gccgctggca
     3001 aacttggtgg gcggcatata cttaaccggc gcctggggaa agagctcgcg agtgagcatg
     3061 gcgtgggcca gctcatagct gaagctgttt tccagctccg gatcgatctc atgggcgtgg
     3121 cccaggccca tcttttctgg cttcacaccg ctcaggaggg caaactgctc gttgaggagc
     3181 tgggaagccg tcacggtgta ggctttctcg atggcgtccg aggtggtgag atagttgtct
     3241 tccccggtct gaatctcgat gccggcatag gcattgatca tacggctgaa ggattggtcc
     3301 aaaagcgtgc ggcggggatt gatatcacga aagaggatgc catacatggc gtcgttgagc
     3361 atcaggtcca gccgttcaat ggcacccata acagcgatct ctggcatgca aagtccggaa
     3421 gcatagttgg tgaggctgat atagcggcct tcggcttcgc tcacttcatc gagggcggcg
     3481 cgcatgatgc ggaagttttc ctgcgtggca taagtgcctc cgaatccgac ggttgtggca
     3541 ccatagggca catagtccag caggctctga gcggtggaac ggatcacggc aatgatatcc
     3601 gcgccttctc tggccgcggc tttggcctgc accacgtcct catggatatt gccggtggcc
     3661 acgatcacat agatagcagg cacgcggcgt gggccaaaac gcctgaacat ctcttcccgc
     3721 ctggctttct ggctgctgat atggcccagc atggtgtctg tgagctcttg caggaggcta
     3781 tatatctcat ccgcaggggc catgggcagg gctgtgatat ccagatgatg ctggcccatc
     3841 gcttccgcta tctgggtagc gctgagatct ttgtttaaca tggcattggc cagacggatg
     3901 gcggcacctt ttcccagcgc gcctttggcc ttgagctggt cggtgaccac attgggcaga
     3961 ggtttgccgt ctttgagagc tccatccagg cccatcattc tgagcacggt gcgctcgatg
     4021 gtgagcgagc tgtaatcctc gaagagccag ccgaatgaac cggtgatctg tcgcgccgct
     4081 tttctggccc ggctcaccag gtcgtcattc aattccagct tgagcatcct tcctccataa
     4141 gtatcttatt tgcagccgaa attttggaag gctgcctttg cgtcaagcaa tatcgtgaaa
     4201 acagccctga agtgacgctc cctggcaggc agtttgcagc gttccgggag tttagctacc
     4261 ctgttttaaa cagagaatga ttttggaagg accaataagg ccgaattga
//
LOCUS       DAOSFN010000177         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_18702_length_4268_cov_8.243295, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000177 DAOSFN010000000
VERSION     DAOSFN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18702_length_4268_cov_8.243295"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLO35_09055"
     CDS             <1..613
                     /locus_tag="PLO35_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH79858.1"
                     /translation="APVGCSVFAYKFFNFDMAQAAHGRAPAVATGMKRARPDMHVFTY
                     QGDGDLAAIGTAEIIHAANRGEHISVFFVNNAIYGMTGGQMAPTTLPEMKTTTSPYGR
                     KTDDIGFPIRVCELLSSLVAPYYIERVSLLSPADIIKAKKAVNKAIQYNKEGRGFTFV
                     EFISTCPTNWGLDPIKSREWAKENMLPFFKPGCFRDKGEGVES"
     gene            610..1152
                     /locus_tag="PLO35_09060"
     CDS             610..1152
                     /locus_tag="PLO35_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH79859.1"
                     /translation="MKTEIICAGFGGQGVLTIGKFIAQAGMKEGKHVSWLPSYGPEMR
                     GGTANVAAVVADEPIASPIVSYPDVLVALNQPSIDKFAPSIRPGGLLIINTNMCPHGC
                     KRTDINIIAAPMNDISIEIGSMRVLNMLAIGIVIGKTGIIKYETMEADLTAFLKAKDP
                     GLLEQNLSAIKRGMELGQNA"
     assembly_gap    1233..1332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1340..2233)
                     /locus_tag="PLO35_09065"
     CDS             complement(1340..2233)
                     /locus_tag="PLO35_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79860.1"
                     /translation="MKNIPLLTTLLLILLSVSGCRLFKQPTLEKIHDVKILKLGVDKI
                     EMELSLSIHNPNCYKLKLDKLNVDLLSKDRVKIGSAALQKTVVIPKRKSNALYFKVSL
                     DTRPTARMISLSNQSVYFYISGKGHGKVLGFGKRFEFEEPYELDLKKYLEELIPRFEA
                     GGENIFKLKRSYVKEMGLTQAELAVEFILMNPFGLGFNLKGFPAEVWLGDTKVGNGNL
                     ASQLRFDENIHSKDGAMIFKISNFKALWSAVKGVVKGEIPYTVKGTVIIDAFGMEIRK
                     PYQYSGTVPVNLSEFILNKLQ"
     gene            complement(2243..4216)
                     /gene="asnB"
                     /locus_tag="PLO35_09070"
     CDS             complement(2243..4216)
                     /gene="asnB"
                     /locus_tag="PLO35_09070"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOH79861.1"
                     /translation="MCGIAGIISLDKQRGFSAGLLRQMCSLIRHRGPDDEGYLCASSL
                     DDAPQGFGGFDSTEAVQARYPLLPEGSQLRMGLGFRRLAIVDLGETGHQPMSDPASGL
                     HIVFNGEIYNYKELRTELEDLGYHFDSHSDTEVILKSYAAWGKGCQSRFNGIWAFAIW
                     DQHARELFCSRDRFGVKPFYFLSQNGLFAFGSEIKQLLPLLKTRTLNLSLLRRLMKIN
                     AMLNYRDETIWQEIRCLEPGQYLTLKDGAVHKDWHYQLDPQGFETSRLSFTEATEAYR
                     ELFLDSLKLQTRADVEVGSCLSGGLDSSAIVCGMAPLKDLPTQTFSAYFGSDPALDER
                     RWIDLVVKQSGSVAHYISPTPEEAWQALYEGTWNNDLPLGAGCPAQDAVTKLASQTGI
                     RVLLDGQGSDELTGGYRHAQYRFMADLLRRGKLSGLSSSLKAHSASKSALETASILVK
                     TGMSALVPESLLYRLELKYLRFDPFNRRFGEHTGKIELSQIQDIKGSRLNSFLYNMIY
                     STSLQTLLHYEDRMAMRHSVESRVPFLDHRLVELAFSLPSSYKIDPPGGKRIHRAAIG
                     TLVPSEIYERRDKSIFGTPFHRDWMRGQLRGEIDALLASKAFRSRGLWDLPQIMKQWH
                     GYLDGKTTQAEMIFNVVALELWFRHFLPDEAIF"
BASE COUNT          910 a         1127 c         1186 g          945 t
ORIGIN      
        1 agcccctgtg ggatgttccg tctttgccta taaattcttc aattttgaca tggcccaggc
       61 tgctcatggc cgtgctcccg ctgtcgccac cggcatgaaa cgcgcccgtc cggatatgca
      121 tgtctttacc tatcagggtg atggagatct cgctgccatc ggtaccgccg agattatcca
      181 tgccgcaaac cggggcgaac acatctccgt gttctttgtc aacaacgcca tctatggcat
      241 gacgggaggt cagatggccc ccaccactct gcctgaaatg aaaaccacca ccagtcccta
      301 tggccgcaag acggatgata tcggtttccc gatccgggtc tgcgaactgc tctcttccct
      361 ggtggcgccc tactatatcg agagagtgtc gctgctctca ccggccgaca tcatcaaagc
      421 caaaaaagcc gtgaacaagg ccatccaata taacaaagaa ggccgcggct tcacctttgt
      481 ggagttcatc agcacctgcc ccaccaactg gggcctcgat cccatcaaat cccgcgagtg
      541 ggccaaagaa aacatgctgc ctttcttcaa gcccggctgc ttcagagaca aaggcgaggg
      601 agtggaatca tgaagactga gattatctgt gccggatttg gcggtcaggg tgtcctgacc
      661 atcggtaagt tcatcgctca ggcagggatg aaagaaggga aacacgtctc ctggctaccg
      721 tcctacggac ccgaaatgcg gggtgggacc gccaacgtgg ccgccgtggt tgcggatgag
      781 ccaattgcct ctcccatcgt gagttatccc gatgtgttgg tggccctcaa tcagccctct
      841 atcgataagt tcgccccctc tatccgtccc ggcggactct tgattatcaa caccaatatg
      901 tgccctcatg gctgcaaacg caccgatatc aacatcatag cggcccccat gaacgatatc
      961 tccatcgaga tcggttccat gcgggtgctg aatatgctgg ccatcggcat cgtgatcggc
     1021 aagaccggca tcatcaaata tgaaaccatg gaagcggacc ttaccgcctt tctcaaagcc
     1081 aaagatccag gcctgctgga gcaaaacctc agcgccatca aacgtgggat ggaactgggc
     1141 cagaacgcct gaactgcttg aaacgcagat agatgataag cgggacctcg gtatggggtc
     1201 ccctttttgt atgtagcgta gatttccaaa acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nncacgcgcc tactgcagct tattgaggat aaactcgctc aggttcaccg
     1381 gcacggttcc gctgtattga tagggtttgc ggatttccat gccgaaggcg tctatgatca
     1441 cggtgccttt gacggtgtag gggatttcgc ccttgaccac gcctttgacg gcagaccata
     1501 aagccttgaa attggatatc ttaaagatca tagccccgtc tttggagtgg atgttttcat
     1561 cgaagcgcag ctgcgaagcc aggttgccat tgcccacttt ggtgtctccc agccagactt
     1621 cggcgggaaa acctttgagg ttgaagccca gtccgaaggg attcatcagg ataaattcca
     1681 ctgccagttc cgcttgcgtg agccccatct ctttgacata gctgcgttta agcttgaaga
     1741 tattctctcc gcccgcctcg aagcgaggga tcagctcttc cagatatttt ttcaggtcca
     1801 gctcgtaagg ctcttcaaat tcaaagcgtt tgccaaagcc cagcactttg ccgtggccct
     1861 tgccggagat atagaaatag acgctctgat tggacaggct gatcatcctg gcggtggggc
     1921 gcgtatccag cgaaaccttg aaatagagcg cgttggactt gcgtttgggg atcaccaccg
     1981 tcttctgcag ggcggcggag cctatcttga cgcggtcttt gctgagcagg tccacattca
     2041 gcttatccag tttgagcttg tagcagttgg gattgtggat gctgagtgaa agctccatct
     2101 cgatcttgtc tacccccagc ttgaggattt tgacgtcgtg gatcttttcc agggtgggtt
     2161 gcttgaacag cctgcatccg ctcacagaga gcaggatgag cagcagggtc gtaaggaggg
     2221 gaatattttt cattttgtta cctcaaaata tcgcttcatc gggtagaaag tggcggaacc
     2281 acagctccag cgccacgacg ttgaagatca tctcagcctg agtggttttg ccgtccagat
     2341 agccatgcca ctgtttcatg atctggggaa ggtcccataa acctctggag cggaatgctt
     2401 tggatgccag cagcgcgtcg atttcacctc tcagctggcc tcgcatccaa tcacggtgaa
     2461 aaggcgtgcc gaagatggac ttatcccggc gttcatagat ctcagagggg accagcgttc
     2521 cgatcgccgc tctgtgaatg cgtttgccgc cgggaggatc gatcttgtag ctggaaggca
     2581 ggctaaaagc aagctccaca aggcgatgat ccaaaaaagg cacccggctc tcgacggagt
     2641 gacgcatcgc catccggtct tcataatgca gaagcgtctg cagtgaagtg ctatagatca
     2701 tattatagag aaaagagttt aacctgctgc ctttaatatc ttgtatctga gagagttcaa
     2761 tctttccggt atgctctcca aagcggcggt tgaaggggtc aaagcgcaga tacttgagct
     2821 ccaaacgata gagcagggat tcgggcacaa gagcgctcat gccggtttta acgaggatcg
     2881 aggcggtctc gagggcggat ttggaggcgg agtgggcttt gagtgaactg ctgagaccgc
     2941 tcagctttcc tcgccgcagc agatctgcca taaaacggta ctgggcatga cggtaaccgc
     3001 ctgtcagttc gtcagatccc tgtccgtcca gcagcactct gatgcccgtc tgggatgcga
     3061 gtttcgtcac ggcgtcctga gcgggacagc ctgctcccag gggcagatcg ttgttccagg
     3121 tgccctcata caaagcctgc caggcttcct caggggtggg ggagatgtaa tgggccacgc
     3181 tgccgctctg cttcaccacc aggtcgatcc agcggcgttc atcgagggcg ggatcgctgc
     3241 caaagtaggc ggaaaaggtc tgggtgggca ggtctttgag gggtgccatg ccgcagacga
     3301 tggcggagga atcgaggccg ccgctgaggc aggagcccac ttccacatcc gctctggtct
     3361 gcagtttgag gctatccaga aagagctcgc gataggcctc ggtggcctct gtgaagctga
     3421 gcctggaggt ctcaaagcct tggggatcaa gctggtagtg ccagtctttg tggacggctc
     3481 cgtctttcag ggtgagatac tgccccggct ccaggcagcg gatttcctgc cagatcgtct
     3541 cgtctcggta gttgagcatg gcgttgattt tcatcaggcg tcttagcagg ctgaggttga
     3601 gagtcctggt tttcagtagg ggcagcagtt gtttgatctc gctgccaaag gcgaagaggc
     3661 cgttctgaga gaggaagtaa aagggtttga cgccaaagcg gtcgcggctg cagaagagct
     3721 cccgggcgtg ctggtcccag atggcgaagg cccagatgcc gttaaatctg gattgacagc
     3781 cctttcccca ggcggcgtag cttttgagga tcacttccgt atcgctatgg ctgtcaaagt
     3841 gatagccgag gtcctcaagc tccgtccgca gctctttgta gttgtagatt tcgccgttga
     3901 agacgatgtg gagaccgctg gcgggatcgc tcatgggctg atgtcccgtt tcgcccagat
     3961 cgacgatcgc cagacgccgg aaacccaggc ccatcctgag ctgagagcct tccggcagga
     4021 ggggatatct ggcctggacc gcctcagtgg aatcgaagcc gccaaagcct tgcggggcgt
     4081 catccaggct cgaagcgcag agatagccct cgtcgtccgg accgcgatgc cggatcaggc
     4141 tgcacatctg ccgcaacagc ccggcagaga agccacgttg cttgtccaat gagatgattc
     4201 cagcgattcc acacataatg ggctcatgct ttcaggaagg gggctggaag tcaagagaat
     4261 tatccgcg
//
LOCUS       DAOSFN010000178         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_18729_length_4262_cov_8.906347, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000178 DAOSFN010000000
VERSION     DAOSFN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18729_length_4262_cov_8.906347"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2745)
                     /gene="nifJ"
                     /locus_tag="PLO35_09075"
     CDS             complement(<1..2745)
                     /gene="nifJ"
                     /locus_tag="PLO35_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOH79862.1"
                     /translation="MAKFKKATMDGNTAAAHVAYAFAEVAAIYPITPSSGMGELSDAW
                     AADGRKNINGTTVDVIEMQSEAGAAGAVHGSLSAGALTTTFTASQGLMLMLPNMHKIA
                     GEMLPTVFYVTARSLAAQSLSIFGDHSDVMSARNTGFALIACNSVQEVMDLGLVAQLA
                     TMKTTIPFLVFFDGFRTSHELQKIEVIDYDTMAALNNPALVEAFRDRALSPDRPRVKV
                     GQQAPDVYFQGRETTNRQYAEATDVIETTMAEVGAVIGRAYKPFDYVGHPEAEHVIVA
                     MGSACETIEEAITYLNEKRGMKLGLVKVRVYRPFSAEHFIRVLPATVKNIAVLDRTKE
                     PGSLGEPLYLDVVSALKDRKDTFITGGRYGLSSKEFTPTMVLATYKHLMAKGFHGFTV
                     GIEDDVTHLSLPLDEELHTVPEGTISLKFWGLGSDGTVGANKNSIKIIGDNTDMYVQG
                     YFQYDSKKSGGITRSHLRFGKSPIHSQYLVAKEDFVACHNQAFIGRFDLLGGIKENGV
                     FLLNSFWDRDEAFYHLTCEMQQAIIDRKIKFYNIDGLKIAEEVGLGGRVNTVMQTAFF
                     LISGVLDRDEAIRLIKESIKKTYSRKGDEVVKMNLDAVDKVNEALVQVDVPATLPDKC
                     TPRKQLVPSDAKGFVLDVIEPIMREEGDRIKVSQMPIDGFVESGTAKLEKRRVAPFAP
                     HWIAENCIQCNQCSFVCPHAAIRAKLIKHEDLKDAPASFNTLKATGADGYQYKVQIYI
                     DDCQSCRVCVNECPKAALVMSPIEDERAKGEQDNYEYFEKLPTDVMATFKETNVKGSQ
                     FKQPLLEFSGACAGCGETPYIKLLTQLYGDRMIIANATGCSSIWGGTFPTIPYAKNKD
                     GKGPAWANSLFEDNAEYGFGMRLALDSHRKLVKLAIETLMDKAIDPALKEAFGYALEH
                     W"
     gene            2938..3891
                     /locus_tag="PLO35_09080"
     CDS             2938..3891
                     /locus_tag="PLO35_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOH79863.1"
                     /translation="MILSTLLLLCGFATVILGAEGLVRGASSLGVLLKVPQIVIGLTV
                     VAFGTSTPELVVNLFSSVEGHGGIVFGNVIGSNLMNLLFILGLAAAIKPVMIRKHSLR
                     IEIPLSLLATLLLAFLVNDRMGGPDETNLLSRTDGLVLLTLFGVFLGYTHWISSRGEA
                     AESGEVKTYSIWLSLLMIAGGLAGLVLGGRLVVDKAVLLAEALGVSQKVIGLTIVAVG
                     TSLPELATSVVAALKGHSDIAVGNVVGSNIFNLLLILGISGLIRPAAYDLSFNTDLVL
                     LSLATLLLIGLAWIKPRRKFNRLKGWVFVSLYIVYLVWLVS"
     gene            complement(3948..>4262)
                     /locus_tag="PLO35_09085"
     CDS             complement(3948..>4262)
                     /locus_tag="PLO35_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOH79864.1"
                     /translation="ENIYPEELEARLNEDDGVLESLVYESEGKIVGRVFLNMEVLDAQ
                     NNLSKMGAPHTERFLSRYLDTLRDNVNKNVASFARIHRLIEQKEPFEKTPTQKIKRFL
                     YQ"
BASE COUNT          819 a         1084 c         1306 g         1053 t
ORIGIN      
        1 ccagtgctcc agagcatagc cgaaggcttc cttcagagca ggatcgatag ccttgtccat
       61 cagggtttcg atggcaagct tcaccagctt gcggtggcta tccagagcca atctcatacc
      121 aaagccgtat tcggcattgt cttcaaagag gctgtttgcc caggcagggc ctttgccgtc
      181 tttgttcttg gcataaggga tggtcgggaa ggtgccgccc cagatggagg agcagcctgt
      241 ggcgttggca atgatcatgc ggtcgccata gagctgggtg agcagcttga tataaggggt
      301 ttcgccacag ccagcgcagg cacctgagaa ctccagcagg ggttgtttga actggctgcc
      361 cttgacgttg gtctccttga aggtggccat cacgtcggtg gggagctttt caaagtattc
      421 atagttatcc tgctcgcctt tggcacgctc gtcttcgatg gggctcatca cgagggcggc
      481 tttggggcat tcgttcacgc agacgcggca gctctggcaa tcatcgatgt aaatctgtac
      541 cttatactga tagccgtcgg cacccgtggc tttgagggta ttgaagctgg cgggagcgtc
      601 cttgaggtct tcatgcttga tcagcttggc gcggatggcg gcatggggac acacgaagga
      661 acactggttg cactggatgc agttttccgc gatccagtgt ggggcaaagg gagccacacg
      721 acgcttttcc agcttggcgg tgccgctttc aacgaagccg tcgatcggca tctgggatac
      781 cttgatccgg tctccctctt cgcgcatgat gggctcgatc acgtccagca cgaagccctt
      841 cgcgtcactt ggcaccaatt gcttgcgggg agtgcattta tccggcaggg tggcaggaac
      901 gtccacctgg acgagggctt cgttgacctt gtccacggcg tccagattca ttttcaccac
      961 ttcgtcgcct ttgcgggaat aggtcttctt gatggattct ttgatgaggc ggatggcttc
     1021 atcgcggtcc agcactccgg aaatcaggaa gaaggcggtc tgcatcacgg tattgaccct
     1081 tccgcccaat cctacttcct cggcgatctt gaggccgtcg atgttataga atttgatctt
     1141 gcggtcgatg atggcctgct gcatttcgca ggtgaggtga tagaaggcct cgtcgcggtc
     1201 ccagaagctg ttgagcagga acacgccgtt ttccttgatg ccgcccagga ggtcaaagcg
     1261 accgatgaaa gcctggttgt gacaggccac aaagtcttct ttggcaacca gatactggct
     1321 gtggatgggg gatttgccaa aacgcagatg gctacgggtg atgccaccgg attttttgct
     1381 gtcgtattgg aaatagccct gcacatacat gtcggtgttg tcaccgatga tcttgatgct
     1441 gtttttgttg gcacccacgg ttccatcgga gcccaggccc cagaatttga ggctgatggt
     1501 gccttccggg acggtgtgaa gctcttcgtc caaaggcagg ctgagatggg tgacatcgtc
     1561 ttcgatgccc acggtgaagc catggaagcc tttggccatg aggtgtttat aggtggccag
     1621 caccatggtg ggggtgaatt ccttgctgga aaggccatag cggccgccgg tgatgaaagt
     1681 gtccttgcgg tccttgagag cgctcactac gtcgaggtag agaggttcgc ccagggaacc
     1741 gggttccttg gtgcggtcca gcacggcgat gtttttcacg gtggctggca ggactctgat
     1801 aaagtgttcg gcgctgaagg gacggtaaac tctcaccttc acgaggccca gcttcatgcc
     1861 gcgcttctca ttgagatagg tgatggcttc ttcaatggtc tcgcaggcgc tgcccatggc
     1921 cacgatcacg tgttcagcct cgggatggcc tacatagtca aagggtttgt aagcgcggcc
     1981 gatcacggct cccacttccg ccatggtggt ctcgatgacg tctgtggctt cggcgtattg
     2041 acggttggtg gtctcgcgtc cctggaaata gacgtccggg gcttgctgac cgaccttgac
     2101 tctgggacga tccgggctca gagcgcgatc gcgaaaagct tccacgaggg cgggattgtt
     2161 gagggcggcc atggtgtcat agtcgatcac ttcgatcttc tgcagttcgt gggaggtgcg
     2221 gaagccgtca aagaagacca ggaaggggat ggtggtcttc atggtggcca gttgggccac
     2281 gaggcccagg tccatcactt cctgcacgct gttgcaggcg atgagggcaa aaccggtgtt
     2341 gcgggcactc atgacgtccg aatggtctcc aaagatagag agggactggg cggccagcga
     2401 acgggccgtc acatagaaaa cggtgggcag catttcgccg gcgatcttgt gcatgttggg
     2461 aagcatgagc atcaagccct gcgaggctgt gaaagtggtg gtgagagcac ccgcggaaag
     2521 agaaccgtgc acggctcccg cggccccggc ttcggattgc atttcgatca catcgacggt
     2581 ggtgccgttg atgtttttgc gtccatcggc ggcccaggca tcggaaagtt ctcccatccc
     2641 ggaggaggga gtgatcggat agatggcggc cacttcggca aacgcgtatg cgacatgcgc
     2701 ggccgcggtg tttccatcca tggtggcctt tttaaactta gccattgagc ataactcctt
     2761 gtagtctatt tactgtatga aaatcatcaa tttatgtcac aggaattttg gggcgggttt
     2821 atgtcaagca aaagcgtacc actcaccagg gccgcggttt ccgaaacagc ctggattaat
     2881 tcttgacgtc tggggctgat ctgcaaacct ggcgagatca actcaaggat tttgccgatg
     2941 atattatcaa cgctgttgtt actttgcgga ttcgcgaccg tgatcctggg cgcggagggc
     3001 ctggtgagag gcgcttcttc tctgggcgtg ctgctcaaag tgcctcagat cgtgatcggg
     3061 ctcacggtgg tggcctttgg cacctccact cccgaattgg tggtaaatct cttcagctcc
     3121 gtggagggcc atggcgggat cgtgtttggc aacgtgatcg gctccaacct catgaacctg
     3181 ctcttcattc tgggattggc ggccgctatc aaacccgtaa tgatccgcaa acacagcctc
     3241 aggatcgaga ttccgctttc gctgctggct actctcctgc tggctttttt ggtcaacgac
     3301 cggatgggcg gaccggatga gacaaatctg ctgagccgca ccgatggact ggtgctgctc
     3361 actctctttg gagtttttct gggttacact cactggatca gcagtcgtgg cgaagcggct
     3421 gaaagcggtg aggtaaagac ctacagcatc tggctgagtc ttctgatgat tgcgggagga
     3481 ctggcaggat tggtgctggg cggcagattg gtggtggata aagcggtgct gttggccgaa
     3541 gctttgggag tatcgcaaaa ggtgatcggg cttaccattg tggcagtggg gacctccctt
     3601 ccggaactgg caacctctgt ggtggccgct ctcaagggcc attccgacat cgccgtcggc
     3661 aatgtggtgg gctctaatat cttcaacctc ctgctcatcc tggggatttc aggattgatc
     3721 cgtcccgcgg catacgacct cagcttcaac accgatcttg tcctgctgag tctggctacc
     3781 ctcctgctga tcggtctggc ctggatcaag cccagacgca agtttaaccg cttgaaaggc
     3841 tgggtctttg tgagcttgta tattgtctat ctggtctggc tggtgagttg aagagagaag
     3901 atgctgccct ctgagacgct taacgcgtaa cgcgtaacgc gtaacgccta ttgatacagg
     3961 aagcgtttga tcttttgggt gggagtcttc tcaaagggtt ctttctgctc aatgaggcga
     4021 tggatacggg cgaaggaagc cacgtttttg ttgacgttgt ctctcagagt gtccaggtag
     4081 cggctgagga agcgttccgt gtgaggggcg cccattttgc tgagattgtt ttgcgcgtcc
     4141 aggacttcca tattcagaaa gactctgccc acgatcttgc cttccgattc atagacgagg
     4201 gattccagca cgccgtcgtc ctcgttgagc ctggcttcca gctcttcggg atagatgttt
     4261 tc
//
LOCUS       DAOSFN010000179         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_18946_length_4227_cov_9.228907, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000179 DAOSFN010000000
VERSION     DAOSFN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18946_length_4227_cov_9.228907"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..1627)
                     /locus_tag="PLO35_09090"
     CDS             complement(317..1627)
                     /locus_tag="PLO35_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOH79865.1"
                     /translation="MSRKQGNIFEDEELKNREVPLAEKLRPSIIDEVLGQHKLIARDS
                     ILRQMIQSGIYHSFILWGPPGTGKTTIARIIEKSTSQRFVHFSAVLSSINDVKAVMKD
                     ADFAHRREGQRTLIFIDEIHRFNKSQQDAFLPYVESGAVILIGATTENPSFEVIPALL
                     SRCHVFVLEMLGEEELQAIVAKGCQELGLEADEIVCGWLARQSGGDARRALNYLELLQ
                     SFLSSDPHPGVSKLAAVLEKKTMFYDKGREEHFNLISALHKSLRGSDPQAALYWLARM
                     LEGGEDPRYIIRRLIRFASEDVGLADPQALTLAIAAKDALLFIGMPEASCALSQLVIY
                     LATAPKSNAADVAYFAAAADARKTSHYGVPLHIRNAPTQLMKELDYGKAYQYDHDFEH
                     HYSYQKYFPDKMAEKTYYTPGEYGFEREIAKRLEWWRKLRDKKE"
     gene            complement(1624..2478)
                     /locus_tag="PLO35_09095"
     CDS             complement(1624..2478)
                     /locus_tag="PLO35_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79866.1"
                     /translation="MAVKDFRIIHRGCYTPGEVSILASPDVKRFSASTQRLIKAAWES
                     AKLDPDLNLFNSRVLSYAGYSKRREGLAVKVQITDYKSFYGTNVCNHATLPKDQLANA
                     LAVCGVVMSKDGAILMGKRSTQVAEDRQHWHVIGGTMELSRNLFGYRVDRQRFERQCL
                     LELDPTLHIMRELNEELGIDPFDVCDIVCMGLGENLLIGKPELLMLVNVALTARQLRE
                     CAPHAIWQGEHSSLLTLPLEDIAMFVHDYPVAPIGKAALFACLGLHLEQENWPRVPLD
                     ELSARIGF"
     gene            complement(2479..2943)
                     /locus_tag="PLO35_09100"
     CDS             complement(2479..2943)
                     /locus_tag="PLO35_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOH79867.1"
                     /translation="MNRKETYLLALEAEVRSQKLYHALAVSFRRPETSVVFQQLVKME
                     KNHEDKVRAAYLKEFPGADHVVNPQPGEYRHLNLKDPAAVLEYAMGREDVARDHYLIL
                     AEDTDEPGLKEMLLLFAREEEQHKEVLLTQLQDMQGALQWFDPSELAGLMED"
     gene            complement(2967..4226)
                     /locus_tag="PLO35_09105"
     CDS             complement(2967..4226)
                     /locus_tag="PLO35_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH79868.1"
                     /translation="MMSKDNHGVSFLAGRYLKGRSRGLISRNHYLTLAGIMLGVMALL
                     CVSSVMNGFREDIGTRIVGTMSEIRISSRGQDGIKDYDKLVQTLEQKGFKASATIRSE
                     LLIKRGTIVLPAVSFGIDPVAHAKVSKALNPVSDNGMGVRQGIIAGRVDPVLLENDGI
                     ILAAGLASTIGAYLGDEVQIISPRMSIPTAFGMIPRVKSFRVIAIFESAMPEYQQSYA
                     YMSLSSARFFSGAQGVDQMELRTPDFTKAESYARQLQSELPDYKVEPWSRFDASLYSA
                     IRFEKMLMFIIMLLMYVIASFNLTGNLLKSIAQKKRELGLLKSIGFTDRDLRKLFLRQ
                     SFILSSIGIGVGLLVATLLLILQTQTGMVKIGSGDGGLVILPVKVMLTDYLIVVLASY
                     LLTFVSVMLPLRRLRQIDPIALIRQTA"
BASE COUNT          941 a         1156 c         1126 g         1004 t
ORIGIN      
        1 atgcgggcat ctgagctggc tgatatagat cagtaaacaa actcatattc tcaattctca
       61 tattctcatt ggttaaagat acactgtttt ctctgctttc actgaagtat ttatatgtct
      121 tggcattgct cgcaccgact gaagtcagtg ctacggccgg ctaaagccag cgttacaaaa
      181 ggtttacagc ttcacatctc cgcaaaatca tctcatttct cattgatgaa aagccctccc
      241 tcaatatctc tcctctgtat cctccgtagc tctgtagtga aaagaaaaac tctgtaactc
      301 agtagtcaga ttcccatcac tcctttttgt cgcgcagttt cctccaccac tccagccgtt
      361 tggcgatctc gcgttcaaaa ccatattccc cgggtgtata gtaggtcttt tcagccattt
      421 tgtccgggaa gtatttctgg tagctgtagt ggtgttcaaa atcatgatca tactggtatg
      481 ctttgccgta atccagttct ttcatcagct gggtgggagc gttgcggatg tggagcggga
      541 cgccgtagtg gctggtcttt ctggcatcag cggcggcggc aaagtaggcc acgtcggcgg
      601 cgttgctttt gggagcggtg gccagataga tcaccaattg agacagcgcg catgaggctt
      661 ccggcatgcc gataaagagc agcgcgtcct tggcagcgat ggccagagtg agggcctggg
      721 ggtctgccaa acccacatcc tcactggcga aacggatgag gcgacggatg atataacgag
      781 ggtcttctcc accctccagc atcctggcca gccaatacaa ggccgcctgg ggatcggaac
      841 cccgcaagga tttgtgcagc gcggagatga gattgaaatg ctcttcgcgg cctttgtcgt
      901 agaacatggt cttcttttcc agcacggcgg ccagcttgga aaccccggga tggggatcgg
      961 agctcagaaa gctctgcagc agttccagat agttcaaagc ccggcgcgcg tcgcctccac
     1021 tctgacgggc cagccatcca cagacaattt catctgcttc gaggcccagt tcctggcagc
     1081 ctttggccac gatggcctgc agctcttcct cgccaagcat ttccagcaca aagacatggc
     1141 atctggaaag cagggccgga atcacttcaa aagaggggtt ttccgtggtg gcgccgatca
     1201 ggatcaccgc tccggactcc acataaggca gaaaggcgtc ctgctgggat ttgttgaatc
     1261 gatggatttc atcgatgaag atcagggtac gctggccctc gcgacggtgg gcaaaatcgg
     1321 catccttcat cacggccttg acgtcgttga tgcttgacag caccgcgctg aaatgcacaa
     1381 agcgctggga agtagacttt tcgatgatgc gggcgatagt ggtcttgccg gtgccgggag
     1441 gaccccaaag gatgaaagaa tgatagattc cgctctggat catttgcctg aggatggaat
     1501 cccttgcgat gagcttatgc tgtcccagca cctcatcaat gatggaggga cgcagctttt
     1561 cggccagagg gacctcgcgg tttttcagct cttcatcttc aaagatgtta ccctgtttac
     1621 ggctcaaaat cctatcctcg cgctgagctc gtcaaggggc accctgggcc agttttcctg
     1681 ctcaaggtgc aatcccaggc aggcaaagag ggccgccttg ccgatcggag caactggata
     1741 gtcgtggaca aacatcgcga tatcttccaa cggcagagtg agtaaggagc tgtgctctcc
     1801 ctgccagatg gcatgcggcg cgcattctct gagctggcgc gccgtgaggg ccacattcac
     1861 cagcatgagc aattccggct tgccgatcag gaggttttca cccaggccca tgcaaactat
     1921 gtcacacaca tcaaaaggat caatacccaa ttcttcattt agttctctca ttatatgaag
     1981 agtgggatcg agctccagaa ggcactgccg ctcaaatcgc tgacggtcaa cacggtagcc
     2041 gaaaagattc ctgctgagtt ccatcgtccc accgatcaca tgccagtgct gtctatcctc
     2101 ggcaacctga gtagagcgtt tccccatcag aattgcgcca tctttggaca tcaccactcc
     2161 gcagaccgcg agcgcgttgg caagctgatc cttgggcagc gtggcgtggt tacatacatt
     2221 ggtgccataa aaagacttgt aatcagtgat ctgtaccttt acagccagtc cctcgcgtct
     2281 ttttgagtag cctgcatagc tcagcacccg gctgttgaac agatttagat ccgggtccag
     2341 tttcgcgctt tcccaggccg ctttgatcag acgctgggtg gatgcgctaa atctcttcac
     2401 atccggagag gccagaatag acacctcacc gggtgtgtaa cagccccggt ggatgatcct
     2461 gaagtcttta actgccaatc aatcttccat caagccagcc agctcagagg ggtcaaacca
     2521 ctgcagagcc ccctgcatat cctgaagctg ggtgagcagc acttctttat gttgttcttc
     2581 ctctctggca aagagcagca gcatctcttt aaggccgggc tcgtcagtgt cctctgccag
     2641 gatcagatag tgatcgcggg ccacatcttc acggcccatg gcatattcca gcaccgcggc
     2701 cgggtcttta agattgaggt gacgatactc tccgggctgg ggattcacga cgtgatctgc
     2761 ccctggaaac tctttcagat aggcggcgcg gactttgtcc tcgtggttct tttccatttt
     2821 gacgagctgc tggaacacca cactggtctc cggtcttctg aaactgacgg ccagagcgtg
     2881 atagagcttc tgggagcgta cttcggcctc gagggccagg agataggtct ctttgcggtt
     2941 cataacatcc tctgtatgtg tttaatttat gctgtttgac ggatcaatgc aatgggatcg
     3001 atctgacgga gtcttctgag cggaagcatc acgctcacga aggtcagcag atatgaggcc
     3061 agcacaacga taagataatc ggtcagcatc actttcacag gcaggatcac caagccgccg
     3121 tccccggagc cgattttcac catgcccgtc tgagtctgca ggatcagcag gagcgtggcc
     3181 accagcagtc cgactccgat cccgatactg ctgaggatga aagactggcg cagaaacagc
     3241 ttgcggaggt ccctatcggt aaagccgatg gatttcagga ggcccagttc gcgtttcttt
     3301 tgggcaatgc tcttgagcag attaccggtg aggttgaagg acgcgatcac atacatcagc
     3361 agcatgatga taaacatcag catcttttca aagcggatgg cgctgtagag gctggcgtcg
     3421 aaacggctcc agggctccac tttataatcc ggaagctcgc tctggagctg gcgggcatag
     3481 ctttccgcct tggtaaaatc aggggtacga agctccattt ggtccactcc ctgggcgccg
     3541 gaaaagaaac gcgcgctgct aaggctcata taggcatagc tttgctggta ttccggcatg
     3601 gcgctttcaa agatggcgat cactctgaag ctcttcacgc gcgggatcat accaaacgcg
     3661 gtgggtatgc tcatccgggg tgagatgatc tgcacttcgt ctcccagata agctccgatc
     3721 gtggaagcca gtcccgcggc caggatgatg ccgtcgttct ccaataaaac agggtccact
     3781 cggccggcaa tgataccctg ccgcactccc ataccgttat cggagacggg attgagagct
     3841 ttgctgactt tggcgtgggc tacgggatcg atcccaaagc tgacggctgg cagcacgatt
     3901 gtgccacgct tgatcaaaag ctcgctgcgg atcgtggcgg aagctttaaa gcctttttgc
     3961 tccagagtct ggaccagttt atcatagtcc ttgatgccat cctggccccg agagctgatc
     4021 ctgatctcag acatcgtgcc cacgatccgg gtgccgatat cttctctgaa gccgttcatc
     4081 acgctgctca cacagagcaa ggccatcaca cccagcatga tgcctgccag agtgagatag
     4141 tgattgcggc tgatcagacc tctggacctg cccttcagat atctgccggc cagaaagctg
     4201 acaccgtggt tgtctttact catcatg
//
LOCUS       DAOSFN010000180         4173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_19278_length_4173_cov_8.526469, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000180 DAOSFN010000000
VERSION     DAOSFN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19278_length_4173_cov_8.526469"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2429)
                     /locus_tag="PLO35_09110"
     CDS             complement(<1..2429)
                     /locus_tag="PLO35_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79869.1"
                     /translation="MKRYILSLLMTALATIAVANMWQTHTNSSHVFDILAEDGDVWFS
                     SWGGVVRIGAPVLKQDVSLSDMRQKEMWNTGNGLISNDIRRIERIGFSSSMWFASDNS
                     GISIVSPLGIQALDSSLGLPSNRVKKIVENQNRILVATYSGLAEYYYLEGVNFPLLLH
                     QYNVQNTNGALLSNELDAMVLAPNRYLWLGSTSGINYVHLDSLAVDSAWHVLSVPFGT
                     GHTLRFAVNDEVLAIATAQSVYLRSSDPAQNGWTSYSAGNQLPNETISDIALNDDNTL
                     WISYGNWNEDTLIYSRNTANLLTSVSESGTVTNYTENANGLKRKSISRIVCSDGYVYL
                     GSWGEGIYRLENGSWAQYLPNSIGFPKVTHINTDLNYEVWISNGSFNHLPTRKSALGA
                     SRWKDGVWQTMNIANSPIHSDNILTIAVDSKNRKWFGTYDVITDESPVGWLFGLTVWD
                     EETDVWMHFTRFGVRYWNHETNTWGPFLPGSPTLMSNTIGYIARDMHDNMLVAGYDRG
                     FSVFNADDELIGEFEIPNSVYQRGIFIYHNGRQYFIGTQSDRGLMIWNDDSIPDKDGP
                     HWITPSPAELSNCEIYGVVSIESPYEGPQHWIAASNGLFMWNEQYWYKYDTAIKRYRY
                     NLSTGLWDNDLLYYADEERLYGSVRTYPTAIYLDPFNRIWIGSLANGISMYDPSTERF
                     TNYYQANSPLLSNHITMFGYEPTEGLLLIGTPDGLNTLRIGRTVKPQTTLQQLKAYPN
                     PFRPAVHSSVLIVNQPTDVMPQGLNSCRVYDSSGALVAKLEETNFSRFEWDGKTSSGK
                     LCASGVYFFVVT"
     gene            complement(2426..3472)
                     /locus_tag="PLO35_09115"
     CDS             complement(2426..3472)
                     /locus_tag="PLO35_09115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOH79870.1"
                     /translation="MENSAILILLAAIFTHLITHLLVPVNIRFSNRFNIVARPSERRI
                     HEKVIPEGGGLSFALPILIVQMLMGLLVFEGPTGLMLIQLAGVEILVLIMGLIDDKYE
                     SKALFKFFSQLGIALLMYYIGFRVEFLTNPLGKDFMLGWISLPATIFWYLAVLNAINL
                     IDGLDGLATGIAVIVCAVLLVVGLREHNLQVLLLSSLLLAGTLAFLVYNFHPARIFLG
                     DTGALFIGLNIAAISTAGAEQFKGITSITLMIPLAVLAVPLIDVVLAVFRRIGMGNIF
                     KADKHHIHHIMLAFGLSQKAISIIVYIVTLLFGLIAIGFSFSSKKVLFSVLLAISALV
                     VIAAYIFMRQERNK"
     gene            complement(3473..>4173)
                     /locus_tag="PLO35_09120"
     CDS             complement(3473..>4173)
                     /locus_tag="PLO35_09120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH79871.1"
                     /translation="VISKSDSARDPLFVKTEGQRDRDFGGTEVLNKDGRSVASNSDST
                     RESIPHANKDVSGSIMVGSTLRFSTQKNILEVILAACEACALEPRLRFVFIGEGEHFQ
                     LCKAIVSSKGLNERILLPGWDSDVSIWLSQMDVFMLYSRWEALPFSIIEANFSGLPVI
                     GSSIPSIKELVNEEVGWLVPLDDRSALVRILTSLPHQAGAISQKGHLARQRIGQLCSY
                     ESMVDAYLKLYRSK"
BASE COUNT         1016 a         1074 c         1130 g          953 t
ORIGIN      
        1 gtcaccacaa agaaatatac tccgctggca cagagcttgc cgctggaagt cttgccatcc
       61 cattcaaagc gggagaaatt ggtctcctca agcttggcca ccagagctcc gctggagtcg
      121 tagacccggc aggaattcag gccctggggc atcacatctg tgggttggtt gacgatcagg
      181 acgctggagt gaactgctgg tctgaaggga ttggggtagg cttttaactg ctggagagtg
      241 gtttgcggtt tcacggtcct gccgatcctg agagtgttga ggccgtcggg agtgccgatc
      301 aggagcagcc cttcggtggg ttcgtaaccg aacatagtga tatggttgga gagcagggga
      361 gaattggcct gataatagtt tgtaaagcgt tccgtgctgg gatcgtacat gctgatcccg
      421 ttggcaaggc tgccgatcca gatgcggttg aaaggatcca gataaatagc ggtgggatat
      481 gtccttacag agccgtagag cctttcttcg tcggcgtagt agagcaggtc gttgtcccaa
      541 agtccggttg agaggttata gcggtaacgt ttgatggcgg tatcgtattt gtaccaatac
      601 tgctcattcc acatgaagag accattgctg gcagcgatcc agtgctgagg tccctcatag
      661 gggctttcga tcgagaccac gccatagatt tcgcaattgc taagctcagc gggactggga
      721 gttatccaat gcgggccatc tttgtcaggg atggaatcgt cgttccagat catcagtccc
      781 cggtcgctct gagtgccgat aaaatactgc ctgccattat gatagatgaa tataccgcgc
      841 tgatagacgc tgttggggat ttcaaactct ccgatcaact catcgtctgc gttaaaaaca
      901 ctgaaaccac ggtcataacc agcgactaac atattgtcgt gcatatctct ggcgatgtaa
      961 ccgatggtgt tgctcatcag ggttggcgag cccggcagaa aaggtcccca ggtgttggtc
     1021 tcatggttcc aatagcggac cccgaagcgg gtaaagtgca tccaaacatc cgtctcttca
     1081 tcccagacgg tcagtccgaa cagccagccc acgggacttt catcggttat aacgtcatag
     1141 gtgccaaacc acttgcggtt tttgctgtcc accgcgatcg tgaggatatt gtctgagtgg
     1201 atagggctgt tggcgatgtt catcgtttgc cagacgccat ctttccagcg gctggcccct
     1261 aacgcgcttt tgcgggtggg cagatgattg aaactgccgt tggatatcca cacctcgtag
     1321 ttgagatctg tatttatatg ggtgaccttg ggaaagccga tcgagtttgg caaatactga
     1381 gcccagctgc cgttttccag acggtagata ccttcccccc aggagcccag atagacgtag
     1441 ccatcggagc aaactatcct ggatatactc ttgcgtttca atccgttggc attttccgta
     1501 tagttggtca cggttccact ctcacttacc gacgtgagca gattggcagt gtttctgctg
     1561 tagatcagcg tgtcttcgtt ccagtttccg tagctgatcc agagggtgtt gtcgtcatta
     1621 agagcgatgt cagaaatcgt ctcgttgggc agttgattcc ccgcgctgta agaagtccag
     1681 ccgttctgag cgggatcgct ggagcgcaga tagacgctct gagctgtcgc gatggccagg
     1741 acctcgtcat ttacggcaaa gcggagcgtg tgaccagtgc caaagggaac gctcaaaaca
     1801 tgccaggcgc tgtccacggc cagagaatcc agatgcacgt agttgatccc agaggtcgag
     1861 cccagccaga gataacggtt gggggccagg accatagcgt ccagttcgtt ggagagcagg
     1921 gccccgtttg tgttttggac attgtattga tgcagcagga ggggaaaatt gaccccttcc
     1981 agataatagt attcagccaa gccgctgtag gtggccacca aaatgcggtt ctggttttcc
     2041 acgatctttt tcacgcggtt ggagggcaga cccagagagc tgtccaaagc ctggatccca
     2101 agcggtgaaa cgatgctgat gccgctgttg tcgctggcaa accacatgct ggaagaaaag
     2161 ccgatccgct cgatacgacg gatgtcgttg ctgatcaatc cgttgccggt gttccacatc
     2221 tccttttggc gcatatccga taatgaaaca tcctgtttta aaaccggagc tccgatcctc
     2281 accaccccac cccaactgga aaaccagacg tcgccgtctt cggcaaggat atcaaacaca
     2341 tgcgagctat tggtatgtgt ctgccacata ttcgcgacag ctatagtagc cagggctgtc
     2401 atcagcaggc taagtatata tcttttcatt tattacgctc ctgacgcatg aagatgtagg
     2461 cggcgatcac caccaaagct gagatcgcca gcaacactga aaacagcacc tttttggacg
     2521 agaaggagaa tccgatggcg atcagcccaa agagcagcgt cacaatatac acgataatcg
     2581 agatcgcttt ctgggaaagc ccaaaggcca gcatgatgtg atggatgtga tgtttgtccg
     2641 ctttgaagat gttgcccatc ccgatcctgc gaaagacggc cagtaccaca tcgatgagcg
     2701 gaacggccag cacggccagc gggatcatca aagtgatcga ggtgatgcct ttaaactgct
     2761 ctgctccggc ggttgagata gcggcgatat tcaagccgat gaaaagcgca cccgtatccc
     2821 ccaaaaagat tctggcggga tgaaagttgt aaaccagaaa ggctaatgtg cctgccagta
     2881 aaagcgatga cagcaacagc acctgcaggt tgtgttcccg caggcccacc accaaaagca
     2941 ccgcgcagac gatcacagcg atcccggtag ccagaccgtc cagtccatcg atgaggttga
     3001 tggcgttcag aaccgccaga taccagaaaa tcgtggcagg cagagaaatc cagcccagca
     3061 tgaagtcctt tcccagagga ttggtgagaa actccacccg gaagccaatg taatacatca
     3121 gaagcgctat tcccaactgg gaaaagaact tgaagagcgc tttactctcg tatttgtcat
     3181 cgatcagacc catgatgagc accagtatct ccactccggc cagctgaatc agcatcagcc
     3241 ccgtgggtcc ttcgaaaacc aataatccca tcagcatctg cacgatcagg atcggcagcg
     3301 cgaaagacag cccccctcct tccgggatca ctttttcatg aatccggcgc tcggaaggcc
     3361 tggctacaat gttgaaacga ttggagaagc gaatattgac ggggacaagc agatgggtga
     3421 tcaggtgagt gaagatcgcc gccaggagga tcaggatcgc gctgttttcc aactacttac
     3481 tccgataaag cttgaggtag gcatccacca ttgactcata agagcacagt tgcccaatcc
     3541 tctgccgggc caaatgccct ttttggctga tggctccggc ttggtgtggc agactggtga
     3601 gtatcctgac cagggcggat ctgtcatcca gggggaccag ccagccaact tcttcattta
     3661 caagctcttt gatcgagggg atggatgaac cgatcacagg cagaccactg aaattggcct
     3721 cgatgatgct gaagggcaag gcctcccacc tggaatatag cataaagacg tccatctggc
     3781 tcagccagat actcacgtca ctatcccagc ctggcagcag gatacgttcg ttgaggcctt
     3841 tggaactcac gatagcctta catagctgaa agtgttcgcc ttcgccgata aagacgaagc
     3901 gcagacgcgg ctccagcgcg caggcttcac aggccgccag gatgacctcg aggatgtttt
     3961 tctgggtgga aaagcgcaaa gtggagccta ccattatgga gccggaaacg tccttgttgg
     4021 catgagggat agattcacgc gtcgaatcgg agttcgacgc tacagaacgt ccgtccttgt
     4081 ttaacacctc tgtccctcca aaatctctgt ctctctgtcc ctctgtttta acaaaaagag
     4141 gatcacgcgc cgaatcggat ttagagatta cag
//
LOCUS       DAOSFN010000181         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_19394_length_4156_cov_7.458912, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000181 DAOSFN010000000
VERSION     DAOSFN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19394_length_4156_cov_7.458912"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /gene="rsmI"
                     /locus_tag="PLO35_09125"
     CDS             <1..629
                     /gene="rsmI"
                     /locus_tag="PLO35_09125"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOH79872.1"
                     /translation="LMAGQDLAVISDAGTPGLSDPAMALVASAISKGFEVIALPGASA
                     LLPALTASGLNRGPFLFLGFLPTQRKTRQQALSKIRDSIYTVVLYEAPHRIFELLNEL
                     WEQCGNRRISLGRELTKLHEEHIRGDLETLREPGSFNPKGEFVLIIEGAEPAQQCSEA
                     QIHELAQLLVDEGIAINPAAKILSRAYGLKRNQAYQLLLDLQPQRQTK"
     gene            626..1255
                     /locus_tag="PLO35_09130"
     CDS             626..1255
                     /locus_tag="PLO35_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOH79873.1"
                     /translation="MNRPYLLFDFDGTIADSIRPLYELMNRLAPRFGREPISWEEFDQ
                     LRDMNAAQIVKHLKVPFSKMASAVPMVLSEYHKIVHELQPCAGIVELLNNLSENKISY
                     ALLSSNKSENVSAFLGRFNLGGFEWVEGTNGILAKHRRLAHQIRKHQLHKNELYYIGD
                     ESRDIEAAKRCKIKVIAVTWGFHTEAHLRAAQPDYLVSEPAEILRIVRP"
     gene            1659..2775
                     /gene="prfB"
                     /locus_tag="PLO35_09135"
     CDS             join(1659..1721,1723..2775)
                     /gene="prfB"
                     /locus_tag="PLO35_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959056.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOH79874.1"
                     /translation="MDYIDYKKQVGEMISQISEIRGYFDLERLETEQSELELVINSPG
                     FWNDQNQARKISKQISIIREEISHIAKLDELRDELQTYLDLLDDDFNESLFREALDAL
                     EAQQTFIEKAEIECYLNEKYDHNDALFTIHAGAGGTEAQDWAEMLFRMYTRWAEKQGF
                     KYNVIDSLPGEEAGLKSVSIEIQGNYAYGMLKAEIGVHRLVRISPFNSQGKRQTSFAS
                     VFVYPEFDDDIEVEIDVKDLKIDTYRASGAGGQHVNTTDSAVRITHLPTNIVVSCQNE
                     RSQVQNKDKAMAILKSRLYQYYEDQREKEKRTLENSKTEIGWGNQIRSYVFQPYQMVK
                     DLRTDHETGQVDKVMDGDLDGFIYAWLKWRAALRMGS"
     gene            2776..3516
                     /locus_tag="PLO35_09140"
     CDS             2776..3516
                     /locus_tag="PLO35_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOH79875.1"
                     /translation="MDYKKLLPKLDKSKLPRHIGIIMDGNGRWAKKRMLPRLQGHSAG
                     AKSIREVTELGVELGLEYLTFYAFSTENWNRPADEVKGLLKMLKERLIKEIPELNEQN
                     IHVQFIGSEAGLDPDYLKEIKSTAAMTDGNTGMKVNIAFNYGGRLEIVEGIKKLYQQA
                     VTQPDLIDRLSPDSFGEWLYTASQPDPDLIIRTSGELRLSNFLLWQSAYAEIYVTETL
                     WPDFDKAEMLKALLHFQKRDRRYGGVKK"
     gene            3513..>4156
                     /locus_tag="PLO35_09145"
     CDS             3513..>4156
                     /locus_tag="PLO35_09145"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOH79876.1"
                     /translation="MSELRQRVMVSVIFIPLLLAALWLGGGYLIAMFSLVSILGGLEY
                     FTMMRGKGHRVAWIWLGLGTALFVALLIWRELDLVFAWVALLALILDAIMDWDENTSV
                     PRVFSISFGLLYTAVLPAMITRIGLDYIGQPILLSLVMMIWIVDSVAYFVGMSFGKHR
                     NVTAVSPRKSLEGFLAGVFVPFLIVIILYFTKVDFLSGWLMALIAIAAGVFGQLG"
BASE COUNT         1044 a         1085 c         1110 g          917 t
ORIGIN      
        1 atcttatggc tggacaggac ctggcagtga tctccgatgc cggcacgcct ggcctttcag
       61 atcccgccat ggcattggtg gcctcagcga tatctaaagg ctttgaggtc atagctttgc
      121 ctggagcgtc ggctctcctg ccagccctca cagccagtgg cctcaaccgt ggtcccttcc
      181 tgtttttggg atttctgccc acgcagcgca aaacgcgcca acaggccctc agcaagatca
      241 gagacagcat ttacactgtg gtgctctatg aagcgcctca tcggatcttt gagctcctca
      301 acgaactttg ggagcagtgt ggaaaccgca ggatcagcct cggcagggaa ctcaccaaac
      361 tgcacgaaga gcacatcagg ggtgatctgg agacgcttcg cgagccaggc agcttcaatc
      421 ccaagggtga gtttgtgctc atcatcgaag gtgcggaacc cgctcagcag tgctctgagg
      481 cacaaatcca tgaactggcc caactgctgg tggacgaagg gattgccatc aatccagccg
      541 cgaaaatcct ctcgcgtgct tatggactga agcgcaatca agcctaccag ctcctcctcg
      601 atctgcaacc gcaaaggcaa accaagtgaa cagaccttat ctgctctttg atttcgacgg
      661 cacgatcgcg gactcgatca ggcctctcta tgagttgatg aaccgcctcg cgccccggtt
      721 tggcagagag ccgatctcct gggaagaatt tgaccaactg agggatatga atgccgccca
      781 gatcgtaaag cacctcaaag tcccattctc aaaaatggcc tcagccgtcc cgatggtcct
      841 cagcgagtat cataagatcg tgcacgaact gcagccctgc gcagggattg tggaattgct
      901 taacaacctc tcagaaaaca agatatccta tgccctcctg agcagcaata agagtgaaaa
      961 cgtcagcgct tttctgggcc gtttcaatct gggagggttc gaatgggtgg aaggtaccaa
     1021 cggcatcctg gccaagcacc gtcgccttgc tcatcagatt cgcaaacacc agctccacaa
     1081 aaacgagctc tattacattg gagacgaatc ccgcgatatc gaagctgcca aacgctgcaa
     1141 gatcaaggtg attgcagtta cctggggctt tcacacggag gcccatctgc gtgccgctca
     1201 gccggattat ctggtttccg aacccgctga aatcctccgg atcgtgagac cctaacatat
     1261 ttgcaagcac ttatcaggca aggaattgga tcagcttttg gaccttcgtc cttgcttgtt
     1321 caccactacc aaagggatac caagccggag ccatcctccc ataatgggct ttgggtccga
     1381 cttgctgcca ggcgggtttc agtcagccgc aagggggctt taagcggccg cgtatcaagc
     1441 tctcggccca cttttgctta tcactcgctc ctcctattcc gaggcagggg atatgtaaag
     1501 gagaggcaca ggagatgcaa aggaggctaa agagaatgat gcatgaagtg gactttcagc
     1561 cactgaacct gaaggcacaa gtcaaattta cgcttgacaa taatcgcctc ctgagcatca
     1621 agaaccaact aagctattta gttaaaggac ttagatatat ggactacatc gattacaaaa
     1681 agcaggtggg agagatgatc tcccaaatct ccgaaatccg gaggttactt tgatctggaa
     1741 cgcctggaaa cagaacagtc agaactggaa ctggtgatca acagccccgg tttttggaac
     1801 gatcaaaacc aggcccgtaa aatctcaaag cagataagca tcatccggga agagatatct
     1861 cacatcgcga agctggatga gctcagagat gagttgcaaa cctatctgga cttactggat
     1921 gacgacttca atgaatcgct cttcagagaa gcactcgacg ctctggaggc ccagcagacc
     1981 tttattgaaa aggccgagat cgaatgctat ctcaatgaaa aatatgatca taacgacgct
     2041 ctgtttacca ttcacgccgg tgccggggga acggaagctc aggattgggc ggaaatgctt
     2101 tttcggatgt atacccgctg ggctgaaaag cagggtttca agtataatgt gatcgattcc
     2161 ctacctggcg aggaagctgg cttgaagagc gtaagcatcg agatacaagg caattacgcc
     2221 tatgggatgc taaaagccga gatcggagtg caccgtctgg tgcgcatctc tcccttcaac
     2281 tctcagggca agcggcagac ctcctttgcg tccgtgtttg tctatcccga gttcgacgat
     2341 gacatcgaag tcgaaatcga cgtcaaagat ttaaaaatag acacttacag ggccagcgga
     2401 gcaggcggtc agcatgtaaa taccaccgat tcagcggtcc gcatcactca cctgcccacc
     2461 aacatcgtgg tgagctgtca aaacgagcgc agccaggtcc agaacaagga caaagccatg
     2521 gccatcctca aaagccgcct ctatcagtat tatgaagacc agcgcgaaaa ggaaaagagg
     2581 accctcgaaa acagcaagac cgagatcggc tggggcaatc agatccgcag ctacgtcttc
     2641 cagccctatc agatggtgaa agatctgcgc accgatcacg agaccggtca ggtggacaag
     2701 gtgatggatg gcgatctgga cggttttatc tatgcctggc tcaaatggcg ggccgctttg
     2761 cggatgggaa gctaaatgga ctataagaaa ctgctcccta agctggataa aagcaagctg
     2821 cccaggcata taggcatcat catggacggg aacggccgct gggccaaaaa acgcatgctg
     2881 ccacgcctgc agggccacag cgcgggagcc aaatcgatcc gcgaggtcac ggagctggga
     2941 gtggaactcg ggctcgagta tctcaccttt tatgcttttt ccaccgagaa ctggaatcgt
     3001 cccgccgacg aggtgaaggg tcttttaaag atgctcaaag aacggctcat caaagagata
     3061 ccggaactga acgaacaaaa cattcacgtt cagtttatcg gttcggaggc gggactcgat
     3121 ccggattatc tcaaagaaat caagtccaca gccgctatga cggatggaaa taccggcatg
     3181 aaagtcaata tcgctttcaa ctatggcggc cgtctggaga tcgtggaagg gataaaaaag
     3241 ctttatcagc aagcagttac acaacccgac ctgattgacc gcctgagccc tgacagcttt
     3301 ggagaatggc tctacacagc ttcccagccc gatcccgatc tcatcatccg caccagtggc
     3361 gagctgcgtc tgtcaaactt tctgctctgg cagagcgcct acgcggaaat ctatgtgaca
     3421 gaaacgcttt ggcctgattt tgacaaggcg gaaatgctga aagcgctact ccattttcaa
     3481 aagcgcgacc gccgctatgg aggggtgaaa aagtgagcga actgcggcag cgcgtgatgg
     3541 tctcggtgat ctttatcccc ttgttgctgg cagctttatg gctgggaggc ggatacctga
     3601 tcgccatgtt ttcgctggtc agcatcctcg gcggcctcga atatttcacc atgatgcgcg
     3661 gcaagggaca tcgcgtggcg tggatctggc tgggcctggg taccgcgctt ttcgtggccc
     3721 tgctgatctg gagagaactt gacctggtgt tcgcatgggt ggcgctgctg gccttgatcc
     3781 tggatgccat catggactgg gatgaaaaca cgtccgtgcc gcgggtgttt tccatcagct
     3841 tcggcctgtt gtacacggct gtcttgccag ccatgattac aagaatagga cttgactaca
     3901 ttgggcagcc catattattg tcgcttgtca tgatgatttg gatcgtggac agcgtggcct
     3961 acttcgtggg aatgagcttt gggaagcatc gcaacgtcac agcggtgagc cctcgcaagt
     4021 ctctggaagg atttttggcc ggcgtgtttg ttccgttcct gatagtgatt atattatatt
     4081 tcaccaaggt cgattttctg tcgggttggt tgatggcgct gatcgcgata gcggcaggcg
     4141 tctttggcca gcttgg
//
LOCUS       DAOSFN010000182         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_19421_length_4152_cov_8.472785, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000182 DAOSFN010000000
VERSION     DAOSFN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19421_length_4152_cov_8.472785"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..1073
                     /locus_tag="PLO35_09150"
     CDS             393..1073
                     /locus_tag="PLO35_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79877.1"
                     /translation="MEIIRTDQLIKDYDLGKVKVRALDGVDMSIEAGEFVAIMGPSGS
                     GKSTLMHIIGCLDSPSSGDYFLDSLNVGQMERRKLAAIRNAKIGFVFQSFNLLPHLNV
                     LKNVELPLMYAGVGSKDRTMKARHILDSVGLSDRLKHKPAELSGGQRQRVAIARAIVN
                     SPSILLADEPTGNLDSSSGGDILEIFNELHRQGNTVIMVTHDSAVAQRADRVIHIRDG
                     KIHNGDIA"
     gene            1057..2289
                     /locus_tag="PLO35_09155"
     CDS             1057..2289
                     /locus_tag="PLO35_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH79878.1"
                     /translation="MAISLRESLHIGLADISTRKVRTIVTVAGIVLGVMCIMVVLAIV
                     NGMNASTIDWMQQRGGLNKIEVQRNWGYDFSRGGDASFTVREVDYLRSQLPPVEAFNP
                     QVSLQDAQIKSGELYFGGNVRGVYPDMQKVEDWPLEQGRFINLDDIEQHRNVIVLGST
                     VAKELYSSRDPLGRYVELNGHQLMVIGVLRSKYWKNQGSETFGGNALEYMNRRAFVPL
                     STMLSKIDPTQTVSSIEIKTLDSEGVKELRKQVEAIVLNLKAGKRLFSVSSAQEEMQQ
                     MQANMKIFSAIFMLIAVISLLVGGIVIMNIMLASVRERTREIGVRMAIGARGWDILLQ
                     FLVQTVLITSLGGVLGILFGWSILGLVGDYLQMTVKASGSMIWVALMVSIGVGLLFGI
                     APAYRASRLDPVTALREE"
     gene            2291..3364
                     /locus_tag="PLO35_09160"
     CDS             2291..3364
                     /locus_tag="PLO35_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HOH79879.1"
                     /translation="MKASQRMKKRGLPAILASYATTGWEDVMSFLRTLANPKKRALII
                     RNSLHFLKVLYQRVTTEGVLKESASLTYITLLGFVPFVTFIFMIAPDLPFLNIKDKFR
                     GVVANNMFPGSAQAIMQYFDEFIARRTAFNIVGFVILIIASYSLFRSIRNTFDRILSM
                     DLAHTQDLISHLIKFFGTLIFGMLIMVMLFSSSSLPVISVVLNLPILKQLNYIVPFFL
                     QFFAMIFLYMLLPSIRISRRSLFRGAFWTTVVWVMVKSLFDVYIYNLTSIQAVYGVMS
                     ALPIFLTWIYFNWVVIIGGIVLVSVLENKDHKGETIRQPRQTVRLTIEMFSNNHLNRR
                     LETLVDKDSLLDVLAEDKEGTKQ"
     gene            3361..>4152
                     /gene="purH"
                     /locus_tag="PLO35_09165"
     CDS             3361..>4152
                     /gene="purH"
                     /locus_tag="PLO35_09165"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOH79880.1"
                     /translation="MKKYALITVSDKTGVEALAMGLEKLGFTILSTSKTAKHLRQFCS
                     EVVEIADLTGFQEILDGRVKTLHPIIHAGILADRDEPSHIKTLQDLSIDHIDVVAVNL
                     YPFAQTRATEGVSHSQIIENIDVGGPTLIRAAAKNYRHVIVLTDPRDYAPTLDLLGSE
                     AGTQEGWRLYLAHKAFELVTHYDSDIADYLSELHVDLLPVTEMPGFMDLNCSLQSKLR
                     YGENPHQKAGFYVTRRDGWKSLHGKELSLNNILDIDSSLRSVRLFE"
BASE COUNT          939 a         1129 c         1137 g          947 t
ORIGIN      
        1 gtaggtgaga tactgcttct tccctgatcg ttgcggctgg gcattggttg atcaggtcgg
       61 acccacctcg gacaaatgcg aagtgggtcc ggcttggtgt ggacctgata gggtgcttgg
      121 gattggggcg gatatggcta tgtcgttgtc ggatagatgg ttcagggtag aatggggcga
      181 cggtcttgcg ggatggttgt ccctgattga ggaataacat gatcggagcc cttctccgca
      241 ttgtgaaatg atatagaaat cttatcatct ctcctctgtc cctccgttcc tctgttttag
      301 aaaaagaaga acctccgctc ccacctcccc gcccgatttt tgttgacaat atgacagagg
      361 cgtttgaact ggcatattat ggtgaaaagt ttatggaaat cataaggaca gatcagctga
      421 tcaaggatta cgacctcggc aaggtcaagg tccgcgctct cgatggagtg gacatgagca
      481 tcgaggcggg ggagtttgtg gccataatgg gcccttccgg aagcgggaaa agcacgctga
      541 tgcacatcat cggttgcctg gacagcccca gctccggaga ttactttttg gattcgctca
      601 atgtgggcca gatggaacgc cgcaagctgg ccgccatccg caacgccaag atcggctttg
      661 tgtttcagag cttcaacctg ctgccccacc tcaacgtgct caagaatgtg gagttgcccc
      721 tcatgtatgc cggggtgggc agcaaagata ggaccatgaa agccaggcac attctggaca
      781 gcgtgggcct cagcgatcgc ctcaaacaca agcctgccga gctatccgga ggtcaaagac
      841 agagggtagc catagcccgc gccattgtaa attcaccttc catcctgctg gcagatgagc
      901 ctaccggcaa cctggacagc tcttccggag gagacatcct ggagatcttc aatgagctcc
      961 acagacaggg caacactgta atcatggtaa cccacgactc cgctgtggcc caacgcgccg
     1021 accgggtcat ccacatccgg gacgggaaga ttcacaatgg cgatatcgct tagggaatcg
     1081 ctgcatatcg gtctggccga tatatccacc cgcaaggtga ggaccattgt cacggtggct
     1141 ggaatcgtgc tgggggtgat gtgcatcatg gtggtgctgg ccatcgtgaa cggcatgaat
     1201 gcctccacca tagactggat gcagcaaaga ggcggactca acaagatcga ggtgcagcgc
     1261 aactggggct atgatttcag ccggggcggg gatgccagct tcacggtccg cgaagtggac
     1321 tatctgaggt cacagctccc tcccgtggaa gcttttaacc ctcaggtgag cctgcaggat
     1381 gcccaaataa aaagcggcga gctctatttc ggaggcaacg tcaggggcgt ctatcccgat
     1441 atgcaaaagg tggaagactg gccattggaa caagggcggt ttatcaacct cgacgatatt
     1501 gaacagcacc gcaacgtgat cgtgctgggc tccacggtag ccaaagaatt gtatagcagc
     1561 cgcgacccct tgggccgcta tgtggagctg aatggccatc agctcatggt gatcggagtg
     1621 ctgagatcca agtattggaa aaatcagggc agcgagacct ttggcggcaa cgctctggag
     1681 tatatgaacc gccgcgcctt cgttccgctc tccaccatgc tcagcaagat cgatcccacc
     1741 cagacggtaa gctccataga gatcaagact ttagacagtg agggcgtcaa ggaactgcgc
     1801 aagcaggtgg aagccatcgt gctcaacctc aaggccggca agcgactctt cagcgtctca
     1861 tccgcccagg aagagatgca gcagatgcag gccaatatga agatcttttc ggccatcttt
     1921 atgctgatcg ccgtgatctc tctgctggtg gggggaatag tgattatgaa catcatgctg
     1981 gcctcggtga gagagcgcac ccgagagatc ggagtgcgaa tggcgatcgg agcccgggga
     2041 tgggacatcc tgctgcagtt tctggtgcag acggtgctga tcacctcgct gggcggcgtg
     2101 ctgggtatcc tctttggctg gagtattttg ggcctggtgg gagactatct gcagatgacg
     2161 gtgaaagcct caggcagcat gatctgggta gcactgatgg tgtcgatcgg agtgggcctc
     2221 ctgtttggca ttgctccagc ataccgcgcc agccgtctgg accctgtgac ggcattgaga
     2281 gaggaatgag atgaaagcct cccaacgtat gaaaaagcgc ggcttgcctg ccattctggc
     2341 atcctatgcc acaactggct gggaggacgt gatgtccttt ctgcgcactc tggccaatcc
     2401 caaaaagcgg gctttgatca tccggaacag tctccacttt ctgaaagtgc tctatcagcg
     2461 cgtcaccacg gagggagtgc tcaaggaatc cgcctcgctt acctatatca ctcttctggg
     2521 ctttgtgccc tttgtgacct ttatctttat gatcgcgccg gacctgccct tcctgaatat
     2581 caaggacaag ttccgtggcg tggtggccaa caatatgttt cccggttccg ctcaggcgat
     2641 catgcaatac ttcgatgaat tcatcgcccg ccgcaccgct ttcaacattg tgggcttcgt
     2701 gatcctgatc atcgcctcct attccctctt ccggtcaatc cgcaacacct tcgaccgcat
     2761 cctcagcatg gacctggccc acactcagga cctgatctct catctgatca agttctttgg
     2821 caccctgatc tttgggatgc tgatcatggt gatgcttttt tcatcaagtt cgctgccggt
     2881 catctcggtg gtgctcaatc tgcccatcct caagcagctc aactatatag tgccgttttt
     2941 cctgcagttc tttgcgatga tctttttata tatgctgctg ccctccatcc ggatcagcag
     3001 acgctcgctg tttcggggtg ctttttggac caccgtggtg tgggtgatgg tgaagagctt
     3061 gttcgacgtc tatatttaca atcttaccag catccaggcc gtgtatggag tgatgtccgc
     3121 attgccgatc ttccttacct ggatatactt taactgggta gtgatcatcg gtggcatcgt
     3181 gctggtaagc gtgctggaaa acaaagatca caagggcgaa acgattcgtc aaccccgtca
     3241 gacggttcgc ctcaccatcg aaatgtttag caacaaccat ctcaaccgca ggctggagac
     3301 ccttgtggac aaagacagcc tgctggatgt gctggcagaa gataaagaag ggaccaagca
     3361 atgaaaaagt acgctttgat cacagtcagc gacaagactg gagtggaagc cctggccatg
     3421 ggcctggaaa aactgggttt caccattctt tccacctcca aaacagccaa acacctgcgc
     3481 cagttttgct ctgaagtggt ggaaatagct gatcttaccg gctttcagga gatcctggac
     3541 ggccgggtca agaccctgca ccccatcatc catgccggga ttctggcaga ccgcgacgaa
     3601 ccctctcaca tcaagactct gcaagacctc agcatcgatc atatcgatgt ggtggcggtc
     3661 aacctctatc cctttgctca gaccagggcc accgaaggcg tatctcacag ccagattatc
     3721 gagaacatcg acgtcggcgg tccaaccttg atcagggccg cggccaaaaa ctaccgccac
     3781 gtgatcgtgc tcacggaccc cagagattac gctcccacgc tcgacctgct gggctctgaa
     3841 gccggcactc aggaaggctg gaggctatac ctggcccaca aggcctttga actcgtcacc
     3901 cactacgact ctgacatcgc tgattacctc tcggagctcc atgtcgatct gctgcccgtc
     3961 acggaaatgc ccggttttat ggacctcaac tgctcgctgc agtccaagct gcgttatgga
     4021 gaaaatcctc atcagaaagc cggattttac gtgacccggc gggacggctg gaagagcctg
     4081 cacggcaagg aactatcgct aaacaacatc ctggatatcg acagctctct gagatccgtg
     4141 cgtctctttg aa
//
LOCUS       DAOSFN010000183         4032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_20264_length_4032_cov_8.132763, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000183 DAOSFN010000000
VERSION     DAOSFN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20264_length_4032_cov_8.132763"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLO35_09170"
     CDS             <1..508
                     /locus_tag="PLO35_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79881.1"
                     /translation="VHFRKGRSYEGYPDFEPKSDAAAWLEYRRPGAAFLLQEYISGLD
                     CDYRVIAIGTRLYVMRRLTREGDFRASGTKLFDFSAQVPEGLLEFAADIAKRFDTPFL
                     SMDIGYQQGRFYLFEYQASHFGTAAIVRSQRFYTRKQGEWQFQEVKSELEKTLAAGLL
                     EYLEIAAH"
     gene            508..1566
                     /locus_tag="PLO35_09175"
     CDS             508..1566
                     /locus_tag="PLO35_09175"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOH79882.1"
                     /translation="MVGSELLYRPIYTAAWKHAGRTNRQTPLAFYCPEEIDLLGFEQV
                     AKHLPPITYISSNHKVSGKLQQAGLSCKGLFSFPKVLIMSRHSLHRFPSDEILAIGMR
                     HGPYHFKRMTKAENYNRFSLYLFSSQADLEAARAIGVKVGQAVGFPRLDPAFDGSITQ
                     NHLKVLSRRLALDPSKPTMLFTATWDASGMSAIDLWFRHLADLSKRWNILVTLHPWIS
                     SKYRKALRHIEGARLVAPADLLKAIMLANVCVGDQSSILAECCALDKGIVTFDTHPAA
                     RSLPEIDELLKNISVRIYRYSELEQACQLFLDEPSLLQEQRQSANLLMFDALDGQAGK
                     RAADAILKHLSDRGISCS"
     gene            1557..2291
                     /locus_tag="PLO35_09180"
     CDS             1557..2291
                     /locus_tag="PLO35_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOH79883.1"
                     /translation="MLIDAHCHLANLNDLMPLRPLLEEAARNRITSFLSSALRKQEVA
                     FYKELSDPRVRFSAGIHPNFDECDLELSDIIELCDHKDIWAVGEIGLDRNGPDLEFQK
                     DMFQAQLELSAQYQLPVVLHIVGHQQEAWELLKQYPLRYLVHGYAGSVEGYRLLASED
                     SRFTISARILRPDKLELLNAVLADGRYLFETDITQYYVHEGEANPLLRLLEVLEQTAA
                     ISGVAKEVLIQTQEINYQYLIEGRHV"
     gene            2284..3015
                     /locus_tag="PLO35_09185"
     CDS             2284..3015
                     /locus_tag="PLO35_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOH79884.1"
                     /translation="MSEPWQSRTALLIGEDGIDKLKRTRVCVIGLGGVGSYAVEALVR
                     AGIGRFVLIDFDTVGTTNLNRQIIALHESIGQPKTEVMKARVLSINTNAEVITHQVFL
                     DQENRAELLRDCDYYIDAIDSLGPKIGLLEYAVRVELKIISVMGAGNRLDPSQIHLDR
                     LDKSHNCPLARRVRKFLRRRGIQGTFPCVYSSELPTMPEEDEDESLKELVIQRGRQRK
                     MIGSISYMPATMGMMAASWVIRQVL"
     gene            complement(3433..3993)
                     /locus_tag="PLO35_09190"
     CDS             complement(3433..3993)
                     /locus_tag="PLO35_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79885.1"
                     /translation="MPDQLHKKAYQMIRSKAEDNTTAQALSGIFGWPITIAADIAVIP
                     LIYVPMWDRIRDIYGRSNINRDVATKIIGGIVSEIFVDIAFDKFMGNIPLLGIYFNAI
                     CAKTMTWRLGTLFAMLSARGEEVESLRIKESMTLIRKMFPQGDMFKFKTPDKAVFVKL
                     LEGLGDVQPEDYNRKVVALLEHMEEM"
BASE COUNT          966 a         1113 c         1014 g          939 t
ORIGIN      
        1 ggtccacttt cgcaagggca gaagctatga gggctatccg gatttcgagc ctaagagcga
       61 tgccgctgcc tggctggaat acaggcgtcc cggtgccgcc tttctgctgc aagaatatat
      121 ctccgggttg gattgcgact accgcgttat tgccatcgga acaagacttt acgtgatgcg
      181 cagactgacc agagagggag atttccgggc cagcgggacc aagcttttcg acttctctgc
      241 ccaggtcccg gaaggcctgc tggaatttgc cgctgatatc gccaaacgct ttgatactcc
      301 ctttctttcc atggacatag gttaccaaca gggccgtttt tatcttttcg agtatcaggc
      361 ctcccacttt ggcacggccg ccatcgttcg ctcccaaaga ttctataccc gcaagcaagg
      421 agaatggcag tttcaggaag taaaatcaga actggaaaaa accctggcag ctggactgct
      481 ggaatatctg gagatcgcag cgcactgatg gtaggttccg agctgcttta cagacccatc
      541 tacactgccg cctggaaaca tgccggccgg actaaccgcc agacccctct cgctttctac
      601 tgcccggaag agattgatct gcttggtttt gaacaggtgg caaagcatct cccgccaatc
      661 acatacatca gctctaacca caaggtctcc ggcaagctcc agcaagccgg cctgtcctgt
      721 aaaggcctct tcagcttccc aaaagtgctg atcatgagcc gccactctct gcacagattc
      781 ccctctgatg aaatccttgc catcggcatg cgccacggtc cctaccattt caagcggatg
      841 accaaagcgg aaaactacaa ccgcttcagc ctctatctct ttagcagtca ggcagatctg
      901 gaagcggcca gagctatcgg cgttaaagtg ggccaggcag tgggatttcc caggctggac
      961 cccgctttcg acggcagcat cactcaaaac catttgaagg ttctctccag aaggctggcc
     1021 ctcgatccct ccaaaccgac tatgctgttc accgccacct gggatgccag cggcatgagc
     1081 gctatagatc tgtggttcag gcatttggcc gatctctcca aacgctggaa tatcctcgtc
     1141 actctgcatc cctggatttc gtctaaatac cgtaaagcgc tgcgacacat cgagggggcc
     1201 agactggtag ccccagccga tctcctgaag gccatcatgc tggcgaacgt ctgcgtgggc
     1261 gatcaaagtt ccatcctggc agagtgttgc gcgcttgata agggcatcgt gaccttcgat
     1321 acccatcccg ccgccaggtc tttgcctgag atagatgaac tgcttaaaaa catatccgta
     1381 aggatctacc gctacagcga gttggagcaa gcctgccagc tctttctgga tgagccctcg
     1441 ctgctgcaag aacagaggca gtcagctaat ctattgatgt ttgacgcctt ggacggacag
     1501 gctggaaaac gggccgcaga tgccatcctc aaacatctgt cagacagagg aatatcatgc
     1561 tcatagacgc tcactgccac ctcgccaacc tcaacgacct aatgcctctg cggcctctgc
     1621 tcgaagaagc tgcccgtaac agaatcacca gctttctctc atccgctctg agaaagcagg
     1681 aagtggcttt ctataaagaa cttagtgatc ccagagtgcg cttcagcgcc ggcatccatc
     1741 ccaacttcga tgaatgcgat ctggagcttt ccgacatcat tgagttatgc gatcacaaag
     1801 acatctgggc ggtgggggag atcgggctgg acagaaacgg tcccgacctg gagtttcaga
     1861 aagatatgtt tcaggctcag cttgagcttt cggctcaata ccagttaccg gtggtgctac
     1921 atatcgtggg tcatcaacaa gaggcctggg aactgctgaa gcaatatccg ctccgctatc
     1981 tggtgcatgg ctatgccggc agtgtggaag gctatcggct tctggccagt gaagatagcc
     2041 gcttcacgat cagcgcgcgt atcctcaggc ccgataagct ggaactgctg aacgccgtgc
     2101 tggccgacgg acgctatctc ttcgagacgg acatcaccca atattatgtg catgagggag
     2161 aggcaaatcc gctgttgaga ctgctggagg tgctggagca gaccgccgcc atttcaggag
     2221 tcgccaagga agtcctcatt caaacacaag agatcaatta ccagtatctt atcgagggaa
     2281 ggcatgtctg aaccctggca gagccgcaca gccctcctga tcggtgaaga cggcatagac
     2341 aaactgaaga gaaccagggt ctgcgtgatc ggcctgggcg gagtgggttc ctacgcggtg
     2401 gaagcgcttg tcagagcggg aattggacgc tttgtgctga tcgatttcga caccgtgggg
     2461 accaccaacc tcaaccgtca gatcatcgcc ctgcatgaaa gcatcggcca gcccaagacc
     2521 gaagtgatga aagcccgcgt cctcagcatc aacaccaatg cggaagtgat tacccatcag
     2581 gtgtttctgg atcaggaaaa cagggctgaa ctcctaaggg attgcgatta ctatatcgac
     2641 gccatcgaca gcttgggccc caagattggg ttgctggaat atgctgtccg cgtagagctt
     2701 aagatcatct ccgtgatggg ggcaggcaac cgtctggacc cctcccaaat ccatctggac
     2761 cgcctcgata aaagtcacaa ttgcccattg gcccgcagag tacgcaagtt cctgcgccgc
     2821 agaggaatcc agggcacttt tccctgcgtg tattcctccg aactgcccac aatgccggaa
     2881 gaagacgaag acgaaagcct caaagagctc gtcatccaga gaggtcgcca gcgcaagatg
     2941 atcggctcga tcagttatat gcccgccacg atgggcatga tggccgcgtc ctgggtgatc
     3001 agacaagtcc tgtgaatggt tttattaaca cagagaaaag cagaggtttg accagagaaa
     3061 agcagaggtt tcttttacag aaaaaagagg aacagaggga aagagttatt tgagggacag
     3121 agatttataa tgagaggtgg tatgtctgaa tgcgcttttt gtcaagttta cccccaattc
     3181 tcaattctca attctcaatt gtcaattctc aattgtcaat tctcaattct ctgtccctct
     3241 atccctctgt tttaaaaaag gaaacctctg cttttctctt ttcaaacctc tgcttttctc
     3301 tgtgttttaa cgttatgcgt tacgggttag cccgctcccc agaccatctc aaccattcat
     3361 gatcagcgag atctgcgtgc caatctgcga aatccgcggg aaatataacc tctaaaccgt
     3421 ctctaccgac ccctacatct cctccatgtg ctccagcagc gccaccactt tgcggttgta
     3481 atcctctggc tgcacatctc ccagtccctc cagcagcttg acaaagacgg ctttatcggg
     3541 agttttgaat ttgaacatat ccccctgggg aaacatcttg cggatcaatg tcatcgattc
     3601 tttaattctc aggctctcta cttcctcgcc ccgggccgag agcatggcga aaagagtacc
     3661 gagcctccag gtcatcgtct tggcgcagat agcgttaaag tagattccca gcagcgggat
     3721 gttgcccatg aatttatcga aggcgatatc cacaaagatt tcgctcacga tcccgccgat
     3781 aattttggtg gccacgtccc ggttgatgtt gctgcgtccg taaatatctc taattctatc
     3841 ccacataggc acatagataa gtgggatcac ggcgatgtcc gccgcgatgg tgataggcca
     3901 accaaagatg ccgctgaggg cctgggcggt ggtgttgtcc tcggctttgg acctgatcat
     3961 ttgatacgct ttcttgtgga gctggtctgg catttcgata cctcaaactt ttttctgata
     4021 atctaagcag at
//
LOCUS       DAOSFN010000184         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_20785_length_3966_cov_8.116339, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000184 DAOSFN010000000
VERSION     DAOSFN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20785_length_3966_cov_8.116339"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLO35_09195"
     CDS             complement(<1..230)
                     /locus_tag="PLO35_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOH79886.1"
                     /translation="MLDQILAIFKPTQPIYLATMDDDQPHLRPMTLIYCKERWYVATG
                     STDAKVRQLASHPKIEFCLPLSDPSGNGYIRG"
     gene            complement(322..1086)
                     /locus_tag="PLO35_09200"
     CDS             complement(322..1086)
                     /locus_tag="PLO35_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH79887.1"
                     /translation="MDISWYGLILAALLLLIPYAIFRHLRLPLTRQMLISFTRMILQL
                     ALIGLVLQFIFKQVNLWLTLGWMLIMISNAVWTIRGRLKLDKAILIRILPMALGTTTL
                     TVMPWIVFLVLRPDPLFNPTFLIPIYGMVLGNSMNSCAQALERFESNLADNWKAYFTR
                     ITLGANLWEATLPVFKKALHASLLPQLLTVASIGVVSLPGMMTGQILGGASPLVAIKY
                     QMMIMISIFSGVSLTDYIAIRLYLHRRFDKHYLPRG"
     gene            complement(1076..1825)
                     /locus_tag="PLO35_09205"
     CDS             complement(1076..1825)
                     /locus_tag="PLO35_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH79888.1"
                     /translation="MSEIQISIRLNSLAHQDKLLLRQVSLDVQSGQKILIVGPTGSGK
                     TSLLHTLNLTNPGYDGHILFHGKDLREHKPEELRGRIMEVMQEPHLEEGSVESALMLP
                     WSYKVHSRLGKPDLSALLEETVRLLQNFGLDASYLQKECGKLSGGEKQRVALVRAMQF
                     QPEVLLLDEISSALDQSTSGIISDCLFNHYPGTIVAISHDPLWQSRWNRIWRLENGGV
                     KEEHRELELGRDNLGPINPELQKGKGDADGH"
     gene            complement(1999..>3966)
                     /locus_tag="PLO35_09210"
     CDS             complement(1999..>3966)
                     /locus_tag="PLO35_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOH79889.1"
                     /translation="ILALFVFAAAMLGGQTNEWRWANTSVGLDEEWGKSIARDINGNI
                     YVTGNFSGTAYFGTHSVTCQTTWPNYNIFVAKMDPAGEWVWAVSAGSASHSNAYSIGV
                     DDFGGVYITGFFYDDFYAGPLSVSSVGECDAYFAKLDSEGFWIWLYRGGGVLGNDIGN
                     DIAIDPIGLVYFVGEYYGTATFYDQTLISNGENDAFIVCIDTDGTWMGASTVGGTSWD
                     EGFGIAVGPYGEIAITGRFMGEMTLGGDTIQTHGSNDVFVAMRNSSGWQWLRRGGGNS
                     YDFGRDVVMDGDGNCYVTGGFSTTADFGPHTLYAGGAIDVFVARLDLYGNWEWCIGAY
                     GGGEDYVFGIGLDGDGNIYIGGLMRYTLRFDYNTLHSTGDDDGFLARIDPSGLWQWAF
                     NIGYLYMDSVRGITVDEAGNVYSAGYYQEFLNLGDFFITDDLIGIWVGKYGPGDVTLP
                     VELSSFSVSPLDNLSALLQWTTQSETGLLGFNVLRHVDDTLDQATVINSSLIMDGVSL
                     GSQTTYSYQDAELGPETSYYYWLESVDMCGNSQFFGPQILRLDDPSQDPVPPTIPAAT
                     ALLDAYPNPFNPSTTLRYSLKTPGTVKISIHNLKGQLVKTFRMVHALPGYYQIPWDGR
                     DLKNQPVSSGVYIYEMRAGKIIDSKKMIMAK"
BASE COUNT          882 a         1050 c         1148 g          886 t
ORIGIN      
        1 atgccgcgga tgtaaccgtt tccggaggga tcacttagcg gcaggcaaaa ctcgatcttg
       61 ggatgggagg ccaactggcg gaccttggca tcggtgctgc cggtggcgac ataccagcgt
      121 tctttgcagt agatgagggt catgggacgc aggtgcggct ggtcgtcgtc catggtggcc
      181 aaatagatgg gctgggtggg cttgaagatg gcgaggattt ggtctaacat ggtgtctcct
      241 tgggtgagat gcttcgcgtg tgagatgctt cggcatgtga gatctgcgct gtgagttcgc
      301 gtaacgcata acgcttaacg cttaacccct tggcagataa tgtttatcaa agcgccggtg
      361 cagatagagc cggatggcga tatagtccgt gaggctgacg ccgctgaaga tggaaatcat
      421 gatcatcatc tggtatttga tcgccaccag aggagacgcg ccgcccaaaa tctggccggt
      481 catcatgcct ggcaggctca ccactccgat cgaggccaca gtgagcaatt gaggcagcag
      541 cgaggcgtgc agggcttttt tgaagacggg cagggtggct tcccagagat tagcgcccag
      601 cgtaatgcgc gtgaagtagg ccttccagtt gtccgccagg ttgctttcaa agcgttccag
      661 ggcctgggcg cagctgttca tgctgttgcc cagcaccatg ccgtagatgg ggatcaggaa
      721 ggtgggattg aagagggggt cgggcctcag caccagaaag acgatccagg gcatcacggt
      781 caatgtggta gtgccgaggg ccatgggcaa gatcctgatc aggatggctt tgtccagctt
      841 gaggcgtccg cggatggtcc acacggcgtt tgagatcatg atcagcatcc agcccagcgt
      901 gagccagaga ttcacctgtt taaagatgaa ctgcagcacc aggccgatga gggccagttg
      961 taagatcatg cgggtaaaag agatcagcat ctggcgggtg agcggcagac gcaaatgccg
     1021 gaagatggca tagggaatca gcagcaagag cgctgccagg atgaggccat accagctaat
     1081 gtccatcagc gtcccccttc cccttctgaa gctctggatt gatcgggccc aggttgtctc
     1141 tgcccagttc cagttcccgg tgttcctctt ttacgccacc gttttccagg cgccagatgc
     1201 ggttccagcg gctctgccag agggggtcgt gcgagatggc cacaatggtg ccggggtagt
     1261 ggttgaagag acaatctgag atgatgccgc tggtgctttg gtccagagcg gagctaatct
     1321 catccaggag caggacttcg ggctggaact gcatggcgcg gaccagggcc acgcgttgtt
     1381 tttcaccgcc ggagagttta ccgcattcct tttggagata ggaggcgtcc aggccaaagt
     1441 tttgcaagag gcggacggtc tcttccagca acgcgctgag gtcgggtttt cccagccggg
     1501 agtggacttt atagctccat ggcagcatca gagcgctttc cacgctgcct tcctcaaggt
     1561 ggggctcctg catcacttcc atgatgcggc ccctcagttc ctccggttta tgttcccgca
     1621 ggtctttgcc gtgaaagagg atatgaccgt catagccggg attggtgaga ttgagggtgt
     1681 gcagcagcga ggtcttgcca ctgccggtgg gacctacgat caggattttc tggccggatt
     1741 ggacgtcaag gctcacctgg cgcagcagaa gcttgtcctg atgggccagc gagtttagac
     1801 ggatgctgat ttggatttcg ctcatgtatt atgtgttccc aataccaatc taggcggaat
     1861 tggggacgcg ggcaacatgt ttgtgcggct ttcagagaac aggagacttc cctgttcacc
     1921 ctgatcattt gacctatctt tttccagcgc gctgatccat gtcagggctt ccggaacgat
     1981 tgggtgggtc tggctgatct attttgccat gatcatcttc ttgctgtcga tgatcttacc
     2041 cgcgcgcatc tcatagatgt acacgccact ggagacgggt tggtttttga gatcgcgtcc
     2101 atcccagggg atttggtaat aaccgggcag ggcgtgaacc atcctgaagg ttttgacgag
     2161 ctggcctttg aggttgtgga tgctgatctt caccgtccct ggtgttttga ggctatagcg
     2221 cagagtggtg ctgggattga agggattggg ataggcgtcc agcaaagctg tggctgcggg
     2281 gatggtggga ggaacgggat cctgggaggg atcatcgaga cggagtatct gaggaccaaa
     2341 gaactgggag tttccacaca tatccacgct ttccagccag taataatatg aagtttccgg
     2401 ccccagctcg gcatcttgat agctgtatgt ggtttgtgaa cccagcgaaa cgccgtccat
     2461 gatcaggctt gaattgatta cggttgcctg gtccagggta tcatcaacat ggcgcaggac
     2521 gttgaaacct agcagaccgg tctcggattg ggtggtccat tgcagcaagg ccgagaggtt
     2581 gtctaagggc gaaacggaga acgaagaaag ctcaaccgga agagtaacgt cgccgggccc
     2641 gtattttccc acccagatac cgatcaaatc gtcggtgatg aagaaatccc ccaggttgag
     2701 aaactcctga taatatccag cggaatacac attcccggct tcatcaaccg tgatccctct
     2761 gacggaatcc atgtacaggt aaccgatgtt gaaagcccac tgccatagcc cgctgggatc
     2821 gatccgggcc aggaaaccgt catcgtctcc ggtggagtga agggtattgt aatcaaatct
     2881 cagtgtgtag cgcatcaatc caccgatata gatgttgccg tcaccgtcca gaccgatgcc
     2941 aaacacataa tcctcaccgc ctccgtaggc cccgatgcac cattcccaat tgccatagag
     3001 gtccagcctg gccacaaaga catcgatggc gccaccggca tacaaggtat ggggcccaaa
     3061 atccgcggtg gtggaaaatc cccccgtcac atagcagttt ccgtccccgt ccatcaccac
     3121 atccctgcca aaatcgtatg aattgcctcc tccgcggcgc agccattgcc aacccgagga
     3181 attcctcata gccacgaaga cgtcgttgga gccatgggtc tggatcgtgt cgccccccag
     3241 ggtcatttcg cccataaacc ttccggtgat ggcgatttca ccgtatggcc ccaccgcaat
     3301 gccaaagccc tcgtcccaac tggtccctcc cacggtggaa gctcccatcc aggtgccatc
     3361 tgtgtcgata caaacgatga aggcatcgtt ttctccgttg cttatcaggg tttggtcgta
     3421 gaaagtggcg gtaccgtagt attcgcccac aaaatacacc aggccaatgg gatcgatcgc
     3481 aatgtcgttg ccaatatcgt ttcccaaaac acctccaccc cgatataacc agatccagaa
     3541 gccctccgaa tccagtttgg caaaataggc gtcgcactct cctaccgatg atacggaaag
     3601 tggcccggcg tagaaatcat catagaagaa tccggtgata tacacgcccc caaaatcatc
     3661 caccccgatg gagtaagcgt tgctgtggga agcgcttccg gcagaaacgg cccacaccca
     3721 ttctccggct gggtccatct tggccacgaa gatgttgtag tttggccagg tggtttggca
     3781 agtgacggaa tgggtgccga aataagcagt cccggaaaag ttaccggtca catagatgtt
     3841 gccgttgata tcgcgggcga tactctttcc ccattcctca tcgaggccca cactggtatt
     3901 ggcccaccgc cattcgttgg tctgccctcc aagcattgca gccgcaaaaa caaacaaagc
     3961 caaaat
//
LOCUS       DAOSFN010000185         3952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_20895_length_3952_cov_8.682320, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000185 DAOSFN010000000
VERSION     DAOSFN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20895_length_3952_cov_8.682320"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PLO35_09215"
     CDS             <1..354
                     /locus_tag="PLO35_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH79890.1"
                     /translation="SIASLIKLYSEQIENIDPGPVEAIRATGASTLQVWRYAVAPQIL
                     APYLAFTIYRWDINIRMATIVGFVGGGGIGLALYQQQQMLAWRNVGLIMWLIALVVWV
                     MDMFSAYIRERLISN"
     gene            366..1673
                     /locus_tag="PLO35_09220"
     CDS             366..1673
                     /locus_tag="PLO35_09220"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOH79891.1"
                     /translation="MPGFNPVEIILKKRDGKSLSPEELSAFITGFLADQIPEYQMSAL
                     LMACYFQGLNPAEIAALTQSYIASGDSLSFDASLAVADKHSTGGVGDKISLMLAPIAA
                     ALGLTVPMISGRGLGHTGGTLDKLEAIPGFRVQYTMAEFKALVEKHGYALVGQSERLV
                     PADKRIYALRDVTATVESPGLITASIMSKKIAEGARHLVIDLKIGSGAFMPNLRRASE
                     LAESLIRTGESFGQKVKVLFTNMNSPLGRAVGNGLETAEAIEYLKGNPLPDTRLITEA
                     LVSRMLLSASLYSSEQEALKAIAEVVSNGKALAKLEEIIRAQNGDPRVIQDYSLLGTA
                     AHRVPVLAPLGGYVHSIDSRAIGYALVKVKAGRMKVSDTLDYGAGALLDPKIGDQVSA
                     GQAIGEVLANDLSAAEEAARAIAAAYAIAEFPKTPEELILGEM"
     gene            complement(2063..3163)
                     /gene="rsgA"
                     /locus_tag="PLO35_09225"
     CDS             complement(2063..3163)
                     /gene="rsgA"
                     /locus_tag="PLO35_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOH79892.1"
                     /translation="MYRLHELGWNDAWESAFGPYRSKGFIPGRISREVKNSYKVIIET
                     GEVAARLSDLLWKAAKNRGMLPAVGDWVALHQKQAADPFIIMEVLLRQSCFSRKAKNT
                     FGRNFSKPGSSDEQVISANVDIVFLVVALDADYKLRKIERYLSLISESRAKAVIILNK
                     ADLCGDYEDKVDEVKAICPQVPVHAISAIDAWGTDIIQSYLNPGLTISLIGSSGVGKS
                     TLINVLLGEDLLDVGEIRQADCRGRHTTARREMVILPQGGVLIDNPGLRDIKIAGHEE
                     SLEQVFQDIVKLEKQCRFSDCRHDTEPGCAIKAAIEAGTLDPERLESYHALRSELASL
                     NIRREQRRKQEDVRIFKENRKAGKVSQKYRQF"
BASE COUNT          857 a         1073 c         1076 g          946 t
ORIGIN      
        1 tctatcgcct cgctgatcaa gctctacagc gaacagattg aaaacatcga tcccggcccc
       61 gtggaagcca tccgcgccac gggagcgtca actctccagg tgtggagata tgccgtggcg
      121 ccgcagatcc tggcccccta tctggccttt actatctacc gctgggacat caacatccgc
      181 atggccacca tcgtgggttt cgtgggtggc ggtggtatcg gtctggccct ctatcaacag
      241 cagcagatgc tggcctggag gaatgtgggc ctgatcatgt ggttgatcgc gctggtggtg
      301 tgggtgatgg acatgttcag cgcctatatc cgtgaaagac tgataagcaa ctgaaggaga
      361 aatacatgcc cggtttcaac cccgtggaga tcatactgaa gaaacgtgac ggaaagagcc
      421 tctctcccga ggagctatcc gcttttatca caggcttttt ggccgatcaa atccccgaat
      481 accagatgag cgcgctgctg atggcctgct attttcaggg cttgaatccc gctgagatcg
      541 ccgccctcac ccaaagctat atcgccagcg gagacagcct cagtttcgac gcctctctgg
      601 ccgtggccga taaacactcc accgggggcg tgggcgacaa gatatccctc atgctggccc
      661 ccatcgcggc ggccttaggt ctcaccgtgc caatgatttc cggtcgcggt ctgggacaca
      721 cggggggcac cctggacaag ctggaagcga ttcccggctt ccgggtccaa tacacgatgg
      781 cggagttcaa agccctggtg gaaaagcacg gctatgccct ggtgggacag tcggaacgcc
      841 tcgtgccggc cgacaagaga atctacgccc tgagggatgt gaccgccacg gtggaaagtc
      901 ccgggctcat caccgccagc atcatgagca agaaaatagc ggaaggagcc cgccacctcg
      961 tgatcgatct caagatcggt agcggagctt tcatgcccaa tctgcgtcgc gcttccgagc
     1021 tggcagaaag cctcatccgc accggcgaga gctttggcca gaaggtgaaa gtgctcttta
     1081 cgaacatgaa ttctcctctg ggccgggccg tgggcaacgg actggaaacc gctgaggcga
     1141 ttgagtatct gaaaggcaac ccgctgccgg acacgcgtct gatcacagag gccctggtga
     1201 gccggatgct actgagcgcc agtctgtatt cctctgaaca agaagcgctt aaggccatcg
     1261 ccgaggtggt ctcaaacggc aaagccctgg ccaaactgga ggagatcatc cgcgcccaaa
     1321 acggcgatcc acgtgtgatc caagactatt ccctgctggg aacagccgct cacagagtgc
     1381 ctgttttggc tcctttggga ggctatgtcc actccatcga ttcccgcgcc attggctatg
     1441 ctttggtgaa ggtcaaagcc ggacgcatga aggtctccga cactctggac tatggcgctg
     1501 gagcgcttct ggaccccaag atcggcgatc aggtttcagc cggacaggcc atcggcgaag
     1561 tgctggccaa cgatcttagc gccgcggaag aggcagccag agcaattgcc gcggcttatg
     1621 ccatcgcgga gtttcccaaa accccggaag agctcatcct gggagagatg taggcaacag
     1681 ctgaaacggc aaacgcctca tttgtctccc tcatatgaga ccgttgaatg atctggatca
     1741 ctggctttag agcttgtgta ttaatattac cttggccgtc ttggagttta aagctccagt
     1801 cctatgtcat tgttatagat tggtttcggg cgctttttaa cgtcaagcaa ggctcggtgc
     1861 gggattttat ctgctgtgtg caacgtctca ggggggggct tcgatgtgct ggttgaagtc
     1921 cggcccctaa actcggggtc gcggatggct ttatcgtgac cgcttgagct ccaacggcac
     1981 cagatttcac caagcccgaa aagatcatag gctcccctcc cgaagagcct atgatgctta
     2041 tatgagatcc aggccggatg gatcagaact gtctgtattt ctggcttact tttccggctt
     2101 tgcggttttc cttgaagatg cggacatcct cctgtttgcg acgctgttct ctgcggatgt
     2161 tcagagaagc cagctcggat cgcagggcgt gatatgactc cagtctttcc ggatcgaggg
     2221 ttccggcctc gatagccgct ttgatggcgc atcccggttc cgtgtcgtgc cggcaatcgc
     2281 tgaagcggca ctgcttttcc agtttgacga tatcctggaa gacctgttcc aggctctctt
     2341 catggccggc gatcttgata tccctcaggc cgggattgtc tatcaggacg ccgccctggg
     2401 gcaggatcac catttctcgg cgggcagtgg tgtgacggcc tctgcaatcg gcctggcgga
     2461 tttctcccac atccagaagg tcttcaccca gcaagacatt gatcagggtg gatttgccca
     2521 ctcctgagga tccgatcagc gagatcgtaa gccctggatt cagatagctt tggatgatat
     2581 ctgtgcccca ggcgtcgatg gcgctgatgg catgcacggg cacctgaggg cagattgctt
     2641 tcacctcatc caccttatcc tcataatcac cgcaaagatc agctttatta aggatgatca
     2701 ccgctttggc gcggctttca ctgatcaggg acagatagcg ctcaattttg cgcagtttgt
     2761 aatccgcgtc cagggccacc accaaaaaca cgatatccac attggcggag atcacctgct
     2821 catcagagga accgggtttg ctgaaatttc tcccaaaggt attcttggcc ttgcgggaaa
     2881 agcagctttg acgcagcagc acttccatga tgatgaaagg atccgccgcc tgcttttgat
     2941 gcagcgccac ccaatctccc acggcaggga gcatgccgcg gtttttggcc gctttccaca
     3001 gtaggtcgga tagcctggcg gccacttccc cggtttcaat tatgaccttg tagctgtttt
     3061 tcacctcccg gctgattctg ccagggatga accctttgct tctatatggc ccaaacgcgc
     3121 tttcccaagc atcgttccag cctaattcat gtaatcggta cacgattcct ccattgtggt
     3181 gagtgattaa ttctgggtgc tgaggaatca agcagcttga ttcccagcta atctttactc
     3241 tgcctctgcg gcacaatttc ctggctcatg ttcactcctt caggctgaaa aaaatagcgc
     3301 tctgaggaac caattcccca gagcgctttc tggaaacaat cgagggaacg gctcattcac
     3361 cgatcccggg atctatatcg tgataccgcg ttcagccttg attcacagta tcttatatga
     3421 tgtcatggta tgagataagg atcggcttta gtctgtgacg ggtccttggg cagtatttct
     3481 aacaggcatg ttaatctcct tatcgcaata ttttgatgcc attgatccat aggggataat
     3541 cgtgtcaagc ttattgtgcc gggcccggga ggataggctg gcgacacttt attccagggc
     3601 agataatcgt ggtattgatg actttaggac gccttgatgt cttttctttt tttagaacag
     3661 agggacagag gaacagagga atggatttct cgggtattgc tatggcatac aagctattac
     3721 aatccattaa atcctgatga tagtgtcatc tgtgttctat cataaatgat gctttgtcaa
     3781 cagcctgagc ccggagggcg gcacaagatt aggctgcagt actggccccc ggaccggaaa
     3841 ctgagcctgg ctttgaataa ttcaggcctg ggtttctcca aaaagaggat tagtcttagc
     3901 ggcccgattt gacttaaaga caggcgggcc tgcgctcaag ttctgtcaga cc
//
LOCUS       DAOSFN010000186         3868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_21551_length_3868_cov_9.566483, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000186 DAOSFN010000000
VERSION     DAOSFN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21551_length_3868_cov_9.566483"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..3751)
                     /locus_tag="PLO35_09230"
     CDS             complement(272..3751)
                     /locus_tag="PLO35_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOH79893.1"
                     /translation="MQKAKLYFFLLLFLLIAYSAWATVSEYSFTSTLGTFTEITGGTV
                     LGTATNDNEVFNALPLGFTFNYNGVDYTEVSVACNGFIAMGPAAVTSNQPISNANSSN
                     NVVAAIGRDIKSRDTGELMYLSSGTAPNRMFTVQWKHWRRSVTATANDDVTFQIQLQE
                     NGNKVVFVYGPFTAVTSTTNQAIQVGLRGDSNADFNNRATTTDWSATTAGTANNNFCV
                     LSASVFPASGLSFTFTAPVAGEPPLPASNPVPANNAINVSIYSNLSWVAGGGTVEGYK
                     VYLGTDNPPTSLVNGTTQTDSSYNPEDFTYNTVYYWKIVPYNSFGDALNCPVWSFTTL
                     TDPTITAFPYNQNFDALTPPTLPLGWNAINVNGDAYTWETYAGNAQSNPNCARIRFNN
                     TAPMNDWLLMPPLQLTQDVYYKVRFYYRGGDVTAAEKLALFWGMAPDPTALTNQIWVN
                     ENINNVSYALGEGIIQAPSTGVFHFGFKAYSPEDLFYIFLDTVNVSIWVEALNPPRNL
                     TATVDNHDVHLAWIAPLESRALLGYKVYRNNTLLATIDNPDSLAYHETGIASGLYSYG
                     VTAFYTSGESVPAGPVLADVDPVILPPANLTATVLERDVTLNWTNPEGNWITWCNMTT
                     GNSVGTNAAAVFDVAHRWPQEDLAPFAGRSLSRIEFVPFFANCTYTIKIWTGGSATDA
                     GTLVHSQVVPSVVLNEWNRVFLTAIIPIPATGDLYYGYEVNTTGGYPAGADDGPPIEG
                     KGNMMYFGGQWTTLSVIAPTLTYNWNIRAFAQFGTPPAGEQMLPPDLTEYMLDVNSLS
                     SQRHQSCPAPRNVTGYKVYRDSILLSTINSEEINTYTDAGLANATYLYSVTSVSPTGE
                     GLPATVEVVVNFQLAEMFFEDGFEDYPDFATTFAPWTLRDVDLSATYGITDVTFPGSG
                     GPMSFIIFNPATTTPPITDLVPHGGAKMAGSFAATTPPNNDFIVTPRVQLGTNSSIKF
                     FARSHTAQYGLERFRVGVSVLPTVIPGGFTYVTGPDYIEAPVTWTEYVYDLSAWDNQR
                     VYVGIRCVSNDAFVFWLDDVSLHSDGGTDADDPVVPALVNALQGNYPNPFNPETTIRF
                     STAQSGRVKIDVYNIKGQLVKQLLNENLPSGKHSVIFNGKDAHGRPVASGVYFYRMQA
                     GNFKASGRMIMMK"
BASE COUNT          882 a          951 c         1125 g          910 t
ORIGIN      
        1 ttatatcagc ctccggcttc gccagttttt gcacatgctc tgaagccagt gataccaatt
       61 attcaacggt cttatctttg tttgaaggag ctttggactt caaacagaac atcgcacccc
      121 aggtatattt gtctcggagc tgctgaccaa gcttgtccct ggtgtccata aacgtctcaa
      181 gcgcgtttat catctccaaa gatcaggcta taagaaaaag cccggggcgg aaatcgcttt
      241 cgccccgggc tttataagga attgcagatg tttatttcat catgatcatg cggccgctgg
      301 ccttgaaatt gccagcctgc atccggtaga aatacacgcc actggccact ggcctgccat
      361 gggcgtcttt tccattgaag atcacgctgt gcttgccgga aggcagattc tcgttcagca
      421 gttgcttcac aagctggcct ttgatgttat agacgtcaat cttgacccta ccgctttgag
      481 cggtgctgaa gcggatggtg gtctccggat tgaagggatt gggatagttg ccctgcaggg
      541 cgttgaccaa ggccggaacg acaggatcat ccgcgtccgt gcctccgtca gagtgcaggg
      601 atacgtcatc caaccagaac acgaaggcat cgttggaaac acagcggatg ccgacataga
      661 ctctctgatt gtcccaggcg gaaagatcat agacatactc ggtccaagtt acaggggctt
      721 cgatatagtc cggcccggtc acataggtaa atccacccgg aatgacggtc ggcaacacgc
      781 tcacgcctac cctgaagcgt tccagaccat actgggcagt atggctgcgg gcaaagaact
      841 tgatcgagct gttggtgcca agctgcaccc tgggggtcac gataaagtcg ttgttgggag
      901 gggtggtagc ggcaaaactg cctgccatct ttgctccccc atgggggacc agatcggtga
      961 tgggtggagt ggtggtggct ggattgaaga tgatgaaaga catcggtcca ccggaacccg
     1021 ggaacgtgac atctgtgatc ccgtaggtgg ctgacaggtc cacatccctg agggtccagg
     1081 gagcgaaggt agtggcaaaa tcaggataat cctcaaatcc atcctcaaag aacatctcag
     1141 ccagctggaa attcaccacg acctccacgg tggcaggcag gccttcacct gtgggtgaaa
     1201 ctgatgtcac ggaatagaga taggtggcat tggcaagtcc ggcgtcggtg taggtgttga
     1261 tttcttcgct gttgatcgtg ctcagcagga tgctgtcacg atagacttta taaccggtta
     1321 cgtttctggg tgccgggcag gactgatgcc gttgggaaga cagggaattt acatccagca
     1381 tatactccgt cagatcagga ggcagcatct gttctccggc cggaggagtc ccaaactggg
     1441 caaaagccct gatattccag ttgtaagtga gggtgggagc tatgacgctg agggtggtcc
     1501 actgcccacc gaagtacatc atattgccct ttccttcgat cggaggtcca tcatcagcgc
     1561 cggcaggata accaccggtg gtgttgacct cgtatccata gtaaagatca ccggtggcag
     1621 gaatgggaat gatggcggtg aggaataccc ggttccattc gttgaggacc acgctgggaa
     1681 ccacctgaga gtggacaagg gtcccggcat cggtggccga gccaccggtc cagattttga
     1741 tcgtgtaggt gcaattggca aagaatggca caaattcgat cctggaaaga gagcggccag
     1801 caaagggagc aaggtcctcc tgaggccatc tgtgggctac gtcaaacacg gccgcggcat
     1861 tggtccccac agagtttccg gttgtcatgt tgcaccaggt gatccagtta ccttctggat
     1921 tagtccagtt cagggttaca tcccgctcca gcacggttgc ggttagattg gcagggggca
     1981 ggattaccgg atcgacatcg gcgagcactg gtccggccgg aactgactca cccgaggtgt
     2041 aaaaggccgt cacgccatag ctgtacaatc cggaagcgat cccggtttcg tgataggcca
     2101 gggagtccgg attgtctata gtggcgagca aggtattgtt gcgatagact ttgtatccca
     2161 gcaaagccct tgattccagg ggtgctatcc aggccaggtg gacgtcatgg ttatcgaccg
     2221 tggcggtgag gtttctgggc ggatttagtg cttccaccca aatggataca ttcaccgtgt
     2281 ccagaaagat gtagaacaag tcctcagggc tgtaggcttt gaaaccaaag tggaaaacac
     2341 cggtggaagg agcttgaatg atcccctctc caagggcata ggagacgttg ttgatgtttt
     2401 catttaccca gatctgattg gtcagagccg tggggtctgg ggccataccc caaaacaggg
     2461 ccagtttctc cgcggcggtg acgtcaccgc cccgataata gaatctcacc ttgtaatata
     2521 catcctgagt gagctgcaga ggaggcatca acagccagtc gttcatcggc gcggtattgt
     2581 tgaaacgtat cctggcgcaa ttgggattag actgagcgtt gccggcatag gtttcccagg
     2641 tataggcatc cccgttgaca ttgatggcgt tccaccccaa aggtagagtc gggggagtga
     2701 gagcatcgaa gttttgattg taggggaaag cagtaatggt gggatcggtc agagtcgtaa
     2761 agctccaaac cgggcagttc agagcatccc caaaactgtt gtacggcact atcttccaat
     2821 agtatacggt gttataagtg aagtcctcgg gattgtagct gctatctgtc tgggtggtcc
     2881 cgttcactaa gctggtggga ggattatcgg ttcccagata gactttgtat ccctccacgg
     2941 ttcccccccc ggctacccag ctgaggttgg aatagatgga cacattgatg gcattgtttg
     3001 cggggacggg attgctggct ggcagaggtg gctcacccgc cacgggagcc gtgaacgtaa
     3061 agctgagtcc actggccggg aagacgctgg cactcaacac gcagaagttg ttgttggcag
     3121 ttcctgctgt ggtggcagac cagtcagttg tcgtggcccg gttgttgaag tcggcgttgg
     3181 aatcaccgcg caggcctacc tgaatcgctt ggttggtggt gctggtgacg gcagtgaaag
     3241 gaccatagac aaacacgact ttgttgccgt tttcctgcag ctggatctga aaagtgacgt
     3301 cgtcgttggc agtggcggtg acagagcgtc gccagtgctt ccactgcact gtgaacatcc
     3361 tgttgggcgc ggtgcctgag ctcaggtaca tcagttcacc tgtgtcccgg ctcttgatgt
     3421 ctctaccgat ggcagccaca acattgtttg agctgttcgc gttgctgatc ggctgattgc
     3481 tggtcaccgc tgcggggccc atggcgatga atccgttgca ggccacgctc acttcagtat
     3541 aatcgacccc gttatagttg aaagtgaagc ccaggggcag agcattgaaa acttcgttgt
     3601 cgttggtagc ggttcccagg accgtgcccc ctgtgatctc tgtgaaggtg cctaatgtgg
     3661 atgtgaacga atattcgctc acagttgccc aagctgaata cgcgatcagc aaaaagagca
     3721 acaggaaaaa atacagcttt gccttttgca tggtaactcc ttcttctttc gaatatctta
     3781 acctctgtga tacttgtaaa tactcttggt gccaaggcac atcaggataa cagcgtggag
     3841 gtctggcaat ttgtgtcaag gaaaaagt
//
LOCUS       DAOSFN010000187         3605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_23880_length_3605_cov_10.169014, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000187 DAOSFN010000000
VERSION     DAOSFN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23880_length_3605_cov_10.169014"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..874
                     /locus_tag="PLO35_09235"
     CDS             68..874
                     /locus_tag="PLO35_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOH79894.1"
                     /translation="MKRLSILLLLLPMLLGLSCGKKKPQSQSPPVAQVSEDILRQDAF
                     RSTFTDEEWNAMDSAQRKKYVEDWVNLTLLAQEADALGISKEPEVRQKLDYASKKVKA
                     NALIARRLADIKISEDELFNYYRIHQADFTGKMLEYNVQRIYLKDQPSATALLERLKT
                     GLDFNEAVRTSSRENLREQLGNMGFVSPADTLFWQQARSLKDGEFGMFAADGGWYLIR
                     HTGTREGEQDAGFEEFRSEIRERILSERQRQVYDELLRELKAKNNEIYYY"
     gene            884..2143
                     /locus_tag="PLO35_09240"
     CDS             884..2143
                     /locus_tag="PLO35_09240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH79895.1"
                     /translation="MRKTLLLLLLLSLAIALPATELLDRIVAKVGSEIILMSDIQKQM
                     LQMQASGLPKESINPSDVIQELIEQRLILQKAKELDIKLNEDAIRKYADGYIKQIKDR
                     YPSEEEFKKELAASQITENELLEFYVEMLRDNALTEQLVERFVTSKAVVTETEMLSFY
                     EAHKDSLAVKPVTWETGMILRNVVASKEAIQARLDEIRAIQQRVMNGEDFATLAAETS
                     DCPSKARGGDLGFFSKGMMVKPFEDAAFKLSVGEYSDVVRSEFGFHLIKVEEKRGEEI
                     RARHILKIIQPTLQDSLDASATMESVRERLLNGAPFGELAAVYSEDLESVDEGGIIGE
                     FDENGMPELFKNVIMATPVGQPTQVLENEGMLYIFMRLKELPQRLYSYDEVKDEVGLI
                     LSRQKQMQAYEEWIATLAKESWVQVLQ"
     gene            2140..2631
                     /locus_tag="PLO35_09245"
     CDS             2140..2631
                     /locus_tag="PLO35_09245"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOH79896.1"
                     /translation="MKPDRLSDPVNMIASLFGTGFLPKAPGTWGTVYALALYLVLPSA
                     LFAGPARWWFTGALLLFCLFSVWISSLAEKRLGHDAPQIVIDELCGFFVSVLWLPKGF
                     LLGLWAFILFRAFDIAKPLVVGWAQKLPKGWGVVADDLAAGLIANLILQGLTRLAPLF
                     FSL"
     gene            2643..2981
                     /gene="yajC"
                     /locus_tag="PLO35_09250"
     CDS             2643..2981
                     /gene="yajC"
                     /locus_tag="PLO35_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOH79897.1"
                     /translation="MLYTLLQAAAPAQTGGGSSSMIFLMIGMFAIMYFLMIRPQQKKQ
                     KELQKMLDALQVNDKVITTSGIYGRVAAIKPDKGIVVIEIDDTNHIKVDFQRSAVAAV
                     ITAGAEPPQA"
     gene            2992..3579
                     /gene="def"
                     /locus_tag="PLO35_09255"
     CDS             2992..3579
                     /gene="def"
                     /locus_tag="PLO35_09255"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOH79898.1"
                     /translation="MKDKPELLPIRIIGDAVLRQKAESVGQITDEIRDFAADLVHTMY
                     LRDGVGLAAPQVGRSLRIFAVDPWWGREGRQKEPLVLIDPVIESSAGECVNEEGCISI
                     PGIYADVARPSSITISFTGLDGQRRKLELDGFAAIVMQHEYDHLDGVLFTDRVSSLTK
                     LKLKRRIKELEKQTVDGVNIRHELPDADPEQHSSL"
BASE COUNT          849 a         1024 c         1005 g          727 t
ORIGIN      
        1 acgcggatgc agaattgtat tcagatttcg ggcgctttgg acttcaagca gggctgggtg
       61 tgaagatatg aaacgcctgt ccatcctcct gctgctcctt cccatgctgc tgggcctcag
      121 ttgcggcaaa aagaagcctc aatcccagtc tcctcccgtg gcccaggtga gtgaggacat
      181 cctccgtcag gatgccttcc gcagcacctt cacggatgaa gagtggaacg ccatggactc
      241 cgcgcagcgc aagaaatacg tggaggattg ggtaaacctc acgcttttgg cccaggaagc
      301 cgacgctttg gggatcagca aagagccgga agtgcggcaa aagctggatt acgccagcaa
      361 aaaagtgaaa gccaatgcct tgattgccag acgtttggca gatatcaaga tcagcgagga
      421 cgagcttttc aattactatc gcatccacca ggccgatttt acgggcaaga tgttggaata
      481 taacgtgcaa aggatttatc tcaaggatca gccttccgcc acagcgctgt tggaaaggct
      541 gaaaaccggg ctcgatttca acgaggcggt gcgcaccagc tcccgtgaaa acctgcgcga
      601 acagcttggc aacatgggct ttgtgagtcc agccgacacg ctgttctggc agcaggcccg
      661 ctccctcaaa gatggggagt ttggcatgtt tgccgctgac ggaggctggt atctgatccg
      721 tcacaccggc acccgggaag gcgaacagga tgccggcttt gaggaattcc gctccgaaat
      781 ccgggagcgt atcctctccg agcgccaacg ccaggtctat gacgagctcc tgcgcgagct
      841 caaggccaaa aacaacgaaa tatactacta ttaggaaact tacatgagaa agaccctctt
      901 actgctcctg ctgctgagcc tggccattgc cctgccggcc acggaactcc tggaccgcat
      961 cgttgccaag gtgggcagcg agatcatcct gatgagcgac atccaaaagc aaatgctcca
     1021 gatgcaggct tccgggctcc ccaaggaaag catcaacccc agcgacgtca ttcaggagct
     1081 catcgagcag cgcctcatcc tccagaaagc caaagaactg gatatcaagc tcaatgaaga
     1141 cgccatccgc aaatatgccg acggctatat caagcagatc aaagaccgct accccagcga
     1201 agaagaattt aagaaagaac tggccgccag ccagatcact gaaaacgaac tgctggaatt
     1261 ctatgtggaa atgctgaggg acaacgccct cacggaacag ctcgtggagc gctttgtgac
     1321 ctccaaggcg gtggtgaccg agaccgaaat gctctccttt tacgaggcgc acaaggacag
     1381 cctggccgtc aaacccgtca cctgggagac cgggatgatc ctgcgcaacg tggtggccag
     1441 caaggaagcc attcaagccc gtctggacga gattcgcgcc atccagcagc gggtcatgaa
     1501 cggggaggat tttgccactc tggcggctga aaccagcgac tgtccctcca aagccagagg
     1561 gggagatctg ggcttcttta gcaaaggcat gatggtcaaa ccctttgagg acgcggcttt
     1621 caagctcagc gtgggtgaat acagcgacgt ggtgcgcagc gaatttggct ttcacctcat
     1681 caaggtggag gaaaaacgcg gcgaggaaat ccgcgcccgt catatcctca agatcattca
     1741 gcccaccctg caggacagcc tggacgcctc ggccacgatg gaaagcgtgc gggaacgcct
     1801 cctgaatggc gctccctttg gagagctggc cgcggtctat agtgaggacc tggagtcggt
     1861 cgatgagggt gggatcatcg gtgaatttga tgaaaacggt atgccggagc tctttaagaa
     1921 cgtaattatg gccaccccgg tgggacaacc cacccaggtg ctggaaaacg agggtatgct
     1981 ctatatcttt atgcggctca aggaactgcc tcagcgcctc tacagctatg acgaggtcaa
     2041 ggacgaggtg gggctgatcc tttcgcgcca gaaacagatg caggcctacg aagaatggat
     2101 cgccaccctg gccaaagaat cctgggtgca ggtattgcaa tgaagccaga ccggctctca
     2161 gacccggtca atatgatcgc cagcctcttt ggcaccggct ttcttcccaa agctccaggc
     2221 acctggggca cggtttacgc tctggcactc tatctggtgt tgccttccgc cttgtttgcg
     2281 ggaccggcca gatggtggtt cacgggagct ttgctgctgt tctgcctctt cagtgtgtgg
     2341 atcagcagcc tggccgagaa acgtctgggc catgacgctc cccagattgt gattgacgaa
     2401 ttatgcggct tttttgtgag tgtcctctgg ttgccgaaag gcttcctgct gggcctctgg
     2461 gcctttatcc tcttcagggc ctttgacata gccaagccac tcgtggtggg ctgggcccag
     2521 aaactaccca aaggttgggg agtggtggca gacgatctgg ccgccgggct catagccaat
     2581 ctcatcctgc aaggcctcac ccgcctggca ccattattct ttagcttata ggagaaccaa
     2641 ttatgctata caccttactg caagccgctg ccccagccca gaccggaggc ggcagctctt
     2701 ccatgatctt cttgatgatc ggcatgtttg ccatcatgta tttcctcatg atccgccccc
     2761 agcaaaagaa gcagaaagag ctgcaaaaga tgctggacgc ccttcaggta aacgacaagg
     2821 tcatcaccac ctccgggatc tatggacgcg tcgctgccat caaaccggac aaaggtatcg
     2881 tagtcatcga gatcgacgac accaaccaca tcaaggtgga tttccagcgc tcagccgtgg
     2941 ccgcggtgat cactgccgga gctgaaccgc ctcaggcata accttttaaa tatgaaagac
     3001 aagcccgaat tgctgcccat ccggatcatc ggtgacgctg tcctcaggca aaaagctgag
     3061 tcggtggggc agatcacaga cgagatcagg gattttgccg ccgacctcgt gcataccatg
     3121 tatctgcggg acggagtagg cctggccgct cctcaggtgg gccgctccct ccggatcttt
     3181 gccgtggacc cttggtgggg acgcgaaggc cgccaaaaag aacccctcgt actcatcgat
     3241 cccgtgatcg aaagctccgc cggcgaatgc gtcaacgaag aaggctgcat cagcattcca
     3301 ggcatctatg ccgatgtggc gcggccttca tccatcacga tctccttcac cggactggat
     3361 ggccagcgcc gcaagctgga actcgatggc tttgccgcca tcgtgatgca gcacgaatat
     3421 gaccatctgg acggggtgct cttcacggac cgcgtttcct ccctcaccaa gctcaagctc
     3481 aagcgccgta ttaaagagct ggaaaaacag accgtggacg gtgtgaatat ccgccatgag
     3541 cttccggacg ccgatccgga gcagcattcg tcgttatgag acgtgaagcg ttatgcgtta
     3601 cgcgt
//
LOCUS       DAOSFN010000188         3556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_24355_length_3556_cov_9.491574, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000188 DAOSFN010000000
VERSION     DAOSFN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24355_length_3556_cov_9.491574"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..798)
                     /locus_tag="PLO35_09260"
     CDS             complement(208..798)
                     /locus_tag="PLO35_09260"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH79899.1"
                     /translation="MNALQLIKSLADESRLQILALLRKQAYCVEDLAELTSLAESTVS
                     AHLKKLQAAGLVYSVKQQYYSVYHLKTGLLKTSLEDLLPHLESQPKDARAILRDKVLK
                     AYFKDGICVRLPVQNKKRWIVYQEVIRRFEPQRDYTEPEINELIKSIHADYCLIRREL
                     VDEGVLAREHGIYRLVDRYWEKPGFYQKIWLMDGSD"
     gene            complement(1027..3189)
                     /locus_tag="PLO35_09265"
     CDS             complement(1027..3189)
                     /locus_tag="PLO35_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79900.1"
                     /translation="MRNLLITMLLFSLTQLTGVIHQVGSFSGGTSNFNQVFVQNELVY
                     LTDNGWTAPEGLYILDASNPGAPVLRGTAPDLGRAYDVEVFGQRAYMAVGMNGFMVFD
                     VSDPSAPTLITRIDDTGPCERLTIGGNRLYVIYDYGFRIYDISNPLPQLLSYCADYDV
                     YDLKIEHDLAYVTGSNPCLRILNVANPNALQTMGTFNGFYSSKSLSKQGDLLYLTDEF
                     NNHCLLNVANPSAPFQTYEFDDMWLKCNGIRNGHLFVVLDQPGTGSKLLKVFNLANPV
                     QPALVSSCVLPTTVHSLYISGNYLYVTVSIYGMLIYDISDVSNPALISTSHIPSDAIQ
                     MELRNNYAYIADADSRLIQVNISDPSNPGLVSQYSLSHPFMDMQMVADTLYVSTSVAG
                     LSVFRIAEDPQPLQNNFQLLGSLPLQSYPRDLLVEDDYVYIANDQYLTIVDASDPAEM
                     QIITQFSDPAMYGYLILAKYQNYLYVGSFGEPLGIIDVSDVHNPLLMGTLGSSAVTAS
                     LEVKGHYLFQTGYDLPILIYDIINPLQPLYVGYIPSVSDQNLLFIHDNWLFASYLVAN
                     SVKCFDISNPLNPVQEWNYVWGLPTWDLEHRDGLLYTCNREFGFSIINTRLLTPVDDP
                     EASPPAERLFSSFPNPFSTSSTIRFELDAASSIRLDVYNLRGQLLRKLYRGSRGPGEH
                     RIDFDGRDDSGRALPSGVYLIKATRENQHEFLRVVLIK"
     gene            3374..>3556
                     /locus_tag="PLO35_09270"
     CDS             3374..>3556
                     /locus_tag="PLO35_09270"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOH79901.1"
                     /translation="MMDILLLVLYLVLLVLNLTDGITTWIFVRPDHYEREANPVARWM
                     FKKLGITFGIILAELLW"
BASE COUNT          852 a          841 c         1005 g          858 t
ORIGIN      
        1 ttttcttgcg gccagagtct caggctctgg atttatgtca agcttagagg ttgagttatt
       61 gaaaaccgcc agcaggttat aacgttcctg tagcttgctg atttacccgc cgtgatgatg
      121 ccgcgcgctc tccggcaaaa aacggcggag gcggcacggc atcatcacgg cgggtgaata
      181 ggcaaggaag cagaaaacgc ggatggatca atcagatccg tccatcagcc agattttctg
      241 gtagaagccg ggtttttccc aatagcggtc caccaatctg tagatgccgt gctctctggc
      301 cagcactcct tcgtccacca gctctctgcg gatcagacaa taatccgcgt gtatcgattt
      361 tatcaattca ttgatttcgg gttctgtgta atcccgctgg ggttcgaagc ggcggatcac
      421 ttcctgatag acgatccagc gctttttgtt ctggactggc aggcgaacgc agatcccgtc
      481 tttgaaatag gccttcagca ccttgtctct gaggatggcg cgggcgtctt ttggctggga
      541 ttcaagatgg gggagcagat cttccaggct ggttttaagc agcccggtct tgagatgata
      601 aacgctataa tactgttgct ttacgctata cacaagaccg gccgcttgaa gctttttgag
      661 atgggcggac acggtggact cggccaggct tgtgagctca gccaggtctt caacgcagta
      721 ggcctgcttt ctcagcaagg ccaggatctg cagccgcgat tcatccgcga ggctcttaat
      781 caattgtagg gcattcattt cagatatcct tttcgtagtt tggcgaaaca aaacatagcg
      841 gcgagtatct gtcaatgtat ttttatggat agcgaaacaa tttgggtgga tggaggccat
      901 aaggctgatt tgacaaggct catatgaggc gagtaaatca atagcaggca aagtatttac
      961 agactggccg accgcttgcc tgtgacagag gtcctcaatg gacctgtgaa aacctcaata
     1021 tccgcctcac ttgatcagga ccaccctcag gaactcgtgc tggttttcgc gggtggcttt
     1081 gattagatag acaccgctgg gcagagctct gccgctgtca tcccttccgt caaaatctat
     1141 cctgtgctcg ccgggacctc ggctgcctct gtagagcttc ctaagcaact ggcccctaag
     1201 gttatagaca tccagtctga tggatgaagc cgcatcgagc tcaaacctga tcgtggagct
     1261 ggtgctgaag ggatttggga aagaggagaa aagacgctcc gcgggtgggc tggcctcggg
     1321 atcatcaaca ggggtcagca atcgggtgtt gatgatgctg aagccaaatt cccggttgca
     1381 ggtatagagc agcccgtccc ggtgttccag gtcccaggtt ggcaggcccc agacgtagtt
     1441 ccattcttga acgggattga ggggattgct gatgtcaaag cacttgacgc tgttagctac
     1501 cagataggag gcgaacagcc agttatcatg aatgaaaagc aggttctgat ctgaaacgct
     1561 gggaatgtag cccacatata aaggctggag gggattgatg atatcataga tcaggatggg
     1621 caaatcataa ccggtctgga agaggtagtg acccttgacc tccaatgacg ctgtgaccgc
     1681 gctggagccc aaagtgccca tcagcagtgg attgtggacg tcgctgacgt caatgatgcc
     1741 aaggggttcg ccaaaggaac ccacgtagag ataattctgg tatttggcca ggatcagata
     1801 gccatacata gcgggatcgc tgaattgggt gatgatctgc atctcagcgg gatcgctggc
     1861 atcgacgatc gtaagatatt gatcattggc gatatagacg tagtcatctt ccaccagcag
     1921 atcgcgggga taggattgca ggggcaggga acccaggagc tggaaattgt tctgcagggg
     1981 ctgcgggtcc tctgcaatcc tgaacacaga aagtcccgcg acggaggtgc tcacataaag
     2041 agtgtctgcc accatctgca tatccataaa gggatgagac agagagtatt gggatacaag
     2101 gcccgggttg gaaggatctg agatgtttac ctggatcaat ctggaatccg cgtcggcaat
     2161 ataggcatag ttgttacgga gctccatctg gatggcatcg gaggggatgt gggaggtgga
     2221 aatcagggcc ggattggaga catcgctgat atcatagatc agcatcccgt agatgctcac
     2281 cgtcacgtag agataattgc cggagatata caacgaatgg accgtggtcg gcagcacgca
     2341 ggagcttacc agagctggct ggactggatt ggccaggtta aagaccttca agagtttgct
     2401 gccggtgcct ggttgatcga gcaccacaaa gagatggccg ttgcggatgc cgttgcattt
     2461 cagccacata tcgtcgaatt cataggtctg aaaaggggcc gaaggatttg ccacattcag
     2521 caggcaatgg ttgttgaact catcggtgag atagaggagg tccccttgtt tgctcagcga
     2581 tttggatgaa tagaatccgt tgaaggttcc cattgtctgg agcgcgtttg ggtttgccac
     2641 attcaggatt ctcagacacg ggttggagcc ggtcacatag gcaaggtcat gctctatctt
     2701 gaggtcatag acatcgtagt ctgcgcaata gcttaacagc tgcgggagcg gattgctgat
     2761 atcgtagatc ctgaagccat aatcgtagat cacatagagc ctgtttccac caatcgtgag
     2821 ccgctcgcaa gggcctgtat cgtcgattct ggtgatcagc gtgggtgcgg atggatctga
     2881 gacgtcaaac accataaatc cgttcatccc aaccgccata tatgctcttt ggccaaagac
     2941 ttccacgtcg taggccctgc ccaggtctgg agcggtacct ctcaggacag gagcgccggg
     3001 attggaagca tccaggatgt agaggccctc gggagcggtc catccgttat cggtcaaata
     3061 cacgagctca ttctgcacaa acacctggtt gaaattggag gttcctccac tgaaagagcc
     3121 cacctgatgg atcacgcctg taagctgggt caagctgaac aacagcatag taatcagtaa
     3181 attacgcatc tcatctccag gagtttcacg atgctgggaa gcagttgaga ccttcccacc
     3241 gcgtttcaga catttttatc tgacctgggt aattgtcaag cttattgtcg caccgcttat
     3301 tcagtttgac tgatataagc aggaaggctc gctttgagct tgacaaacaa gggctcatca
     3361 ttcagatagg gtcatgatgg atatcctgct gttggtatta tacttagtgc tgctggtgct
     3421 caatctgacg gacggcatca ccacctggat ctttgtgcgt cccgatcatt atgagcggga
     3481 ggccaatccc gtggcccgct ggatgttcaa aaagctgggg atcacctttg gcatcatcct
     3541 ggcggaactg ctctgg
//
LOCUS       DAOSFN010000189         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_24370_length_3554_cov_9.239783, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000189 DAOSFN010000000
VERSION     DAOSFN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24370_length_3554_cov_9.239783"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="PLO35_09275"
     CDS             complement(<1..1458)
                     /locus_tag="PLO35_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH79902.1"
                     /translation="MKRILWLIVLLLSLVELTALRVDLNAGDSFVKVQQTADNEIELR
                     IRIAAVDIDSDGKIKLPDEFRSHSRGSAIPIWDFHLALPGNGEIETTIEEHRTRLNIP
                     SIMVSKPGTKCVTIGKPYWFRDIRGVDVMISPLRMSDGEVQISDRISVKIKLLKEGAD
                     PGEARSSQRINPYFLDIYKHHFLNFSYRYEDLAEYGSMAVICPVTSYDTQVQRFRGLI
                     QPWVDWKNQMGIPSTVYNVTQIGTTYEDIRAFIQDLYDNDPHLTFVQLVGDFAQVPCN
                     VTTIMGNTGGMDAFYSLLHGDDGYPDVFVGRFSAENAAELYTQIKRSLEYATATTSGN
                     WLSRAAGVCSSNPPIPGDDDEHNWEHLDSLRVQLLDFGYSQVERIYGNEGADTQDLVD
                     CLNSGISLLNYCGEGYPDHWVEPEFGIMEAEALSNTDMLPFIHAVSCWTGQFYDGTCL
                     AEALMRSRDPGSEEARGAIAIYTAAPEQGVAPPMEA"
     gene            1920..2168
                     /locus_tag="PLO35_09280"
     CDS             1920..2168
                     /locus_tag="PLO35_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH79903.1"
                     /translation="MNKKHLLITLALILGLVLIAATQGKPFVDRDKCVTCQDCVSVCP
                     TGAIAIVDGRAQIDPEKCIDCKICVKTCAYRAIRGPQP"
     gene            2165..2416
                     /locus_tag="PLO35_09285"
     CDS             2165..2416
                     /locus_tag="PLO35_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH79904.1"
                     /translation="MKRKALISLLLLCIFILCLASCKRLTITEYSIDSASCNGCGECL
                     RGCPADAIYLGTDGKAHIDQSKCTRCGDCVVLCPQSAIY"
     gene            2392..>3554
                     /locus_tag="PLO35_09290"
     CDS             2392..>3554
                     /locus_tag="PLO35_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79905.1"
                     /translation="MSSKRYLLILLLLGSMVGLLAQPRLSMDAGLYLDDELELKARNR
                     ISATGSFGQGWNWELQSDLHRSRGQSAAQLEELTHAGKAELGYQSGLGTYRLGYTHSV
                     FGESSLLGLYPAWMPALNYKRLMAHQASFSSHNAYDDWSLDAYGIYRHLRVEPYSFDF
                     GDFQFHPQDPEGYSDAFGGAKLSWDASNAVKVYTAMEIKAGFHAEEDPYDYSTVMLGC
                     EGLYKPLPQGRLEGSVQISHRDGDAIDAPKANLVRARLRYQHTFMPGLSGFITWENNT
                     CFADSLSALYLISNYLRPQLVYTLDYDPSGMSYLRLGGKYSPENEASAVFGDGEARLW
                     KGLYASLGAGLIPERLEQYSSALLYRFSSSELGIRYLHREFIIYPGKTDYWGV"
BASE COUNT          805 a         1022 c          926 g          801 t
ORIGIN      
        1 agcttccatg ggtggagcaa cgccctgttc gggagccgcg gtatagatgg caattgctcc
       61 tctggcctct tctgaaccgg gatcccggct tcgcatcaag gcttcggcca gacaggtgcc
      121 gtcataaaac tggcccgtcc aacaggaaac cgcgtggata aagggcagca tgtctgtatt
      181 gctgagggct tccgcttcca taatcccaaa ctccggctcc acccagtgat cgggatagcc
      241 ctctccgcaa tagtttaaga gcgaaatccc ggagttgagg caatccacga ggtcctgggt
      301 atcggcccct tcattgccat aaatccgttc cacttggctg tagccaaagt ccagcaactg
      361 gacccgaagg ctgtccagat gctcccaatt atgctcatca tcgtctccgg gaatgggagg
      421 attggaagaa cagactcccg ccgctctgct tagccaattg cctgatgtag ttgcggttgc
      481 gtattccagg ctgcgtttga tctgggtata gagctccgcg gcgttttcag cggaaaacct
      541 gcccacaaag acatccgggt agccatcgtc gccatgcagc aaggagtaga aagcgtccat
      601 gccgcccgtg ttgcccatga tggtagtcac attgcagggt acctgagcaa aatctcccac
      661 tagttgcacg aaggtgagat ggggatcgtt gtcatagagg tcctggataa aagcgcggat
      721 atcctcataa gtagttccta tctgggtcac attatagacg gtgctgggga tacccatctg
      781 gtttttccaa tccacccagg gctggatcaa acccctaaag cgttgcacct gtgtatcata
      841 gctggttacg gggcagatca ccgccatgga accatactcg gccaggtctt cataacgata
      901 gctgaaattg aggaaatgat gcttgtagat atccaggaaa tagggattga tccgctgtga
      961 tgatcttgct tctccaggat ccgcgccttc cttgagtagc ttgattttta cgctgatccg
     1021 gtctgagatc tgcacttctc catccgacat ccggagcggt gaaatcatca catccactcc
     1081 acgtatatcc ctaaaccagt atggtttacc tatggtcaca catttcgtgc caggcttgga
     1141 gaccatgatg gacggaatgt tcagacgagt ccgatgctcc tctattgtgg tctcaatctc
     1201 tccgttgccg ggaagcgcta aatgaaagtc ccagatggga atagccgatc cacgggagtg
     1261 ggacctaaac tcatccggga gcttgatctt gccatcactg tcgatatcca ccgccgcgat
     1321 cctgatccgc agctcgatct cgttatcagc agtctgctgc acctttacaa aactatcacc
     1381 cgcgttcaaa tccacccgca gggctgtgag ctccaccaat gagagcagca aaacaatgag
     1441 ccacaaaatc cttttcaccg ttctgctccg ttgaggctgg cgtaacccat ttggatctca
     1501 agcggccacg ataaagccat gcctggcagc gagtttgcgg gccgcttgga cttcaaccag
     1561 cccacccgca cccagaggtc agatccaaac cagctttgaa cacgcctcct tcgaataaaa
     1621 tccattgcct tgctcctttg cctgtggcgc ttagaatcaa gatgttctct actattctga
     1681 aggcattccc ctgtcaagct caaattccgg ttcttaatct cctgcgcgtc acctgtgccc
     1741 cacgcgtgct ccacccatgc caaagcacgg gataagcgtg cgataagcac gggataagca
     1801 tgggtggcta agcggaaaga attgctggac agaattctgg gggcgcggaa aagtgcgatc
     1861 tgagattggg tggagcttcc caaagagaac ccgcctgtca tatgagtgtt gggagaacga
     1921 tgaacaagaa acacttactg atcacgctgg ccttgatcct aggcctggtc ctgatcgccg
     1981 ctactcaggg caaaccattt gtggaccgcg ataaatgcgt gacctgtcag gactgcgtga
     2041 gtgtctgccc caccggcgcg attgccatcg tggacggtcg ggcccagatc gatccggaga
     2101 agtgcataga ctgcaagata tgcgtgaaga cctgcgccta ccgtgccatc cgaggaccgc
     2161 agccatgaaa cgcaaagcat tgatatcatt gctgttactt tgtatattca tcctttgcct
     2221 ggcctcctgc aaacggctca ccatcacgga atattccatc gattccgcct cctgcaacgg
     2281 ctgtggagaa tgcctgcgtg gctgtcctgc cgatgccatc tatttgggta cggacggcaa
     2341 agcccacatc gatcaaagca aatgcacccg ttgcggtgac tgcgtggtat tatgtcctca
     2401 aagcgctatc tattgatcct gctgctgctt ggcagcatgg tcgggctgct tgcccagcct
     2461 cgtttgagca tggacgcggg gctctatctg gatgatgaac tggaacttaa ggcccgcaac
     2521 cggatctccg ccaccggctc ctttggccag ggctggaatt gggaactgca atccgatctc
     2581 caccgctcca gaggccaaag cgctgcccaa ctggaggagc ttactcacgc cgggaaagcc
     2641 gaactgggct accaatcagg cctggggact tatcggctgg ggtacaccca cagcgtcttt
     2701 ggtgaatcct ccctgctggg cctctaccca gcctggatgc cagccctgaa ctataaacgc
     2761 ctcatggccc atcaggccag cttttccagc cacaacgcct atgacgactg gagtttggat
     2821 gcctatggaa tctatcgcca cctcagggtg gagccctaca gcttcgactt tggcgatttc
     2881 cagtttcatc cccaggaccc tgaaggctac agcgatgcct ttggcggagc taaacttagc
     2941 tgggatgctt cgaacgccgt caaggtctat accgccatgg agatcaaggc cggcttccac
     3001 gccgaagagg atccctacga ttacagcacc gtgatgcttg gctgcgaggg actttacaaa
     3061 cctctgccgc agggacgtct ggaaggaagc gtgcaaatca gccaccggga tggcgacgcc
     3121 atcgacgctc ccaaagctaa tctcgtaagg gcccggctgc gttatcaaca taccttcatg
     3181 cctggcctca gcggttttat cacctgggaa aacaacacct gctttgccga cagcctcagc
     3241 gccctctacc tgatctcaaa ctatctgagg ccacaactgg tttacaccct ggattacgac
     3301 cccagcggga tgtcctatct gaggctgggt ggcaaataca gccccgaaaa cgaagccagc
     3361 gctgtgtttg gagatggcga agccaggctc tggaaaggcc tctatgccag cctgggcgcg
     3421 ggcttgatcc cggaacgcct ggagcagtac agctccgcgc tgctctacag gttctcctcc
     3481 agcgagttgg gtatccgcta tctgcaccgg gaattcatca tttatcccgg caaaactgat
     3541 tattggggag tgga
//
LOCUS       DAOSFN010000190         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_25853_length_3410_cov_7.481669, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000190 DAOSFN010000000
VERSION     DAOSFN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25853_length_3410_cov_7.481669"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..634
                     /locus_tag="PLO35_09295"
     CDS             122..634
                     /locus_tag="PLO35_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOH79906.1"
                     /translation="MIRVGLLLILAGLMMACGRDFRTVKKVDLQSFMGDWYVIAILPN
                     IIEKNAYNGIESYQLNPDGTIGVTYTFFKGGPQGKKKVMHPLGKVFDRQSNAHWKMRL
                     IKPFWSDYLIVDLAPDYRYTVIGVPNRKFVWIMSRTPDLNDEDYSGIIKRLATSGYRT
                     DKIRKMPQIW"
     gene            640..>1598
                     /locus_tag="PLO35_09300"
     CDS             640..>1598
                     /locus_tag="PLO35_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOH79907.1"
                     /translation="MKISYLDGIRFYRGLLAGGKAVIQNRAELDRINVFPVADADTGI
                     NLTLTMQSILEQARLGASLRDTFRSVADAALEGSRGNSGIIFAQYLYGLSRELPEKGV
                     LNPRSFSEGAQRAVKHLYDSLLNPVEGTMLTVIREWSDFLVEQSAKTQDFMHLLTASL
                     EAARRSLMDTPRRLKVLADAGVVDAGASGFVYFLQGVVDFIHKGSLREIGKAAAHVRL
                     EALPESHGTQPGQNRYCTEAILSGCERGANFVKGVLASHGDSLILAGDREKLHLHIHT
                     ARPDKFFEELYRLGQVSGAKIDDMLRQYQISHERRFEIGLITDT"
     assembly_gap    1599..1698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1699..2203
                     /locus_tag="PLO35_09305"
     CDS             <1699..2203
                     /locus_tag="PLO35_09305"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH79908.1"
                     /translation="RQLSVSEGLVTLRVAKAVEAGLSYDEIMASAPEWAKNTRIYTDV
                     HTLKYMVRGGRVPPLAGFVANLLNVKPIVGLNDQGKAKIWGKSFSRAANARKITALIE
                     NDLKSFRLWDYAIVHAEAEARAQMYARMLEKLTGRPPAYIMPLSPVVGVHNGIGAVAV
                     GVSYDLL"
     gene            2190..2966
                     /locus_tag="PLO35_09310"
     CDS             2190..2966
                     /locus_tag="PLO35_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOH79909.1"
                     /translation="MTFFEILPCAFLAVFVYMNLLFVTALLRKRNDIVDIAWGPGFLI
                     VGAVSLISASSLHWRLLLSYALVGLWALRLALHIWLRNKAKTEDFRYAQWRKDWGKTW
                     VLRSWLQIFMLQGFFMLTVSYPLILQAGSHKDQLLATDLLGLLIWAMGFFFETVGDEQ
                     LRRFKQNPANKGKIMDRGLWSLTRHPNYFGEASMWWGVFVISLGASHGIWAIFSPIII
                     TWLLVKVSGVPMLEKRYQTDPVYREYVKRVSGFVPWKKKA"
BASE COUNT          742 a          917 c          933 g          718 t
ORIGIN      
        1 ccatcagctg gaacttcacc aagtttctga tctccaaaga cggtcgcgtg atcgatcgct
       61 tcgcgcccac cgtgaatccc tcctcggaaa aggtggttca ggccatcgaa gcagccttaa
      121 aatgatccgg gtcggcttac tgttgatcct ggcgggattg atgatggcct gcgggcggga
      181 ctttcgcacc gtcaaaaagg tggacctgca gagcttcatg ggggattggt acgtgatcgc
      241 gatcctgccc aatatcattg agaaaaacgc ctacaacggc atcgaaagct atcagctcaa
      301 tccagacggc accattggcg tcacctatac ctttttcaag ggcgggcccc aaggtaaaaa
      361 gaaagtgatg catcctctgg gcaaggtgtt tgaccgccag agcaacgcgc attggaagat
      421 gaggcttatc aagcctttct ggagtgatta tctgatcgtg gacctggccc cggactatcg
      481 ttataccgtg atcggggtgc caaaccgcaa gtttgtgtgg atcatgagca ggactccgga
      541 tctcaatgac gaggattatt ccggcatcat caagcgcctg gccacttcag gctatcggac
      601 ggacaagatc aggaaaatgc cccaaatctg gtaattcata tgaagataag ctatcttgac
      661 gggatcagat tctatcgtgg cctgctggct ggagggaaag ccgtgatcca aaaccgcgcc
      721 gaactggaca ggataaacgt ctttccagtg gcggatgccg atacgggtat caatctcacc
      781 ctgacgatgc aatccatcct ggagcaagcc aggctgggag cctccctgcg ggacaccttt
      841 cgcagcgtgg ccgatgccgc tctggaaggg tccagaggca attccgggat catctttgcc
      901 caatacctgt atggcctcag ccgggaattg ccagaaaaag gcgtcctcaa tccccgcagt
      961 ttctcagagg gggcccagcg cgccgtcaaa cacctctacg actccttgct caaccccgtc
     1021 gagggtacca tgctgacggt aatcagggaa tggagcgact ttctggtgga gcaaagcgcc
     1081 aagacccaag actttatgca tcttctcacc gcctcgttgg aagcggccag acgctccctt
     1141 atggatacac cccgccggct caaagtcttg gccgatgccg gagtcgtgga tgccggcgcc
     1201 agcggatttg tctatttcct gcagggagtg gtggatttca tccacaaagg ctcgctccgg
     1261 gaaattggga aagcggccgc gcatgtcagg ctggaagcgc ttcccgagag tcatggcacc
     1321 cagccaggcc aaaaccgcta ttgcacggaa gccatcctca gcggttgtga acggggcgcc
     1381 aactttgtga agggagtgct ggccagccat ggagacagcc tcatcctggc cggcgaccgt
     1441 gagaagctgc acctgcacat ccacactgcc aggccggaca agttttttga ggagctctac
     1501 cgtctgggcc aggtgagcgg ggccaagata gacgacatgc tgcgccagta tcagatcagt
     1561 cacgagcgcc gctttgagat cggcttgatc accgacacnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnncc gccagctttc cgtctcggaa ggccttgtga cccttagagt
     1741 tgccaaagcc gtggaggcgg gcctctccta tgacgagatc atggcctcgg ctcctgaatg
     1801 ggccaaaaat acccgcatct acacggacgt ccacaccctc aaatacatgg tccggggtgg
     1861 cagagtgccg cctctggctg gttttgtggc caatctgctc aatgtgaagc ccatcgtggg
     1921 cctcaacgat caaggcaagg ccaaaatctg gggcaagagc ttcagtcggg ctgccaacgc
     1981 ccgcaagatc actgccttga tcgagaacga cttaaagagt ttccgcctct gggattacgc
     2041 catcgtccat gccgaagcgg aagcgcgcgc ccaaatgtat gcccggatgc tggaaaagct
     2101 cacgggaagg cctccggcct atattatgcc gctttctccg gtggtgggag tccacaatgg
     2161 catcggggcc gtagcggtgg gagttagtta tgaccttctt tgagatcctg ccctgcgcct
     2221 ttctggcagt gttcgtctac atgaatctgc tctttgtgac ggccctgctg cgcaagcgca
     2281 acgacatcgt cgatatcgcc tggggacccg gcttcctgat cgtgggagcg gtaagcctca
     2341 tatccgccag ctctttgcac tggcgtctcc tgctcagcta tgctttggtg gggctctggg
     2401 ccttgcgcct ggccctgcat atctggctgc gcaacaaagc caaaaccgag gatttccgct
     2461 atgcccagtg gcgcaaagac tggggcaaaa cctgggtgtt gaggagttgg ctgcagatct
     2521 ttatgctgca gggattcttt atgctcaccg tctcctatcc ccttatcctg caagctggca
     2581 gccataaaga ccagctattg gcaacggacc tgctgggcct gctgatctgg gccatgggat
     2641 tcttttttga aaccgtggga gacgagcagt tgcgccgctt caaacaaaac cccgccaaca
     2701 agggcaagat catggaccga ggcctctggt ccctcacccg ccatcccaac tactttggcg
     2761 aggccagcat gtggtgggga gtgtttgtga tcagcctggg cgccagccat ggcatctggg
     2821 ccatcttcag tcccatcatc atcacctggc tgctggtgaa agtctccgga gtgccgatgc
     2881 tggaaaaacg ctatcagacc gatcccgtct atcgggaata tgtgaagcgg gtgagcggct
     2941 ttgtgccttg gaagaaaaag gcttaggtga gagccgcgcc atgatcgcac aagccatgaa
     3001 gagcttgtga aaaaaaacta ccttggccgt cttggagttc aaagccccta aatagattat
     3061 gcgtctatct ctatacccga cggggctttg gacttacgtg caggacatcg gagcgagtga
     3121 ttcccccatc ggtgtactgg ttgaagtcca agcggcccgc aaactcgctg tcatgtatgc
     3181 ctttatcgtg gccgcttgaa ctccaacggg gctttgttag tctctggttt catcagtttt
     3241 cgcacaagct ctgaagccaa tgttacggac ggctaaagcc agtgatacga acggctgaag
     3301 agcttgtgca aaaagtggca ttttgagttc cgtaggaacg caggctgttg acaaagtatc
     3361 aaatcagata gaacgcagat gacgggatga cacgatcatc aagattccgg
//
LOCUS       DAOSFN010000191         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_26430_length_3359_cov_8.570521, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000191 DAOSFN010000000
VERSION     DAOSFN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26430_length_3359_cov_8.570521"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /gene="rmuC"
                     /locus_tag="PLO35_09315"
     CDS             complement(<1..1661)
                     /gene="rmuC"
                     /locus_tag="PLO35_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOH79910.1"
                     /translation="MIALLALNLALGIILLLVLLRRSGPEDTLSQRFESMSAGLESLG
                     KQVREENLGLRSELGRLNTEQRMELNQNIGSLSEGLKNENRGHREELTSAQNNLSESL
                     SRRMKELSEAQHNGLETLRTSIETRLEQIRANNETRLEAMRRTVDEKLHETLEKRLTD
                     SFKLVSERLEEMQKSSALETRSNREELNTTLKALREQINKDSLTNREELSKALNNLAE
                     SLTRRVQDLSSTQQTSFDTLKTTLDARMEQIRSNNETKLEEMRRTVDEKLHETLEKRL
                     GEHFNQVSDRLEKVHRTMGEVQSLTKSVGKLEQIMGNVKNRGVMGEVQLATLLQDIML
                     PGQYETNFKPRARSNETVEFAIRLPGPDEDQKSVYLPMDSKFPVEDYQHLLDAYEAND
                     PIQIVSSRKAMADRIKACARDIRDKYINPPVTTDFGLLFLPFEGLYAEALRITGLFET
                     LIREQRVVICGPTTTAALVNSLQMGFRTLAIQKKTAEAWKVLTAVKHDFGKFATLLDQ
                     TQKKLHEASKHIETATDRTRQIEKKLSKVQELPDSKTEPVLELDSP"
     gene            complement(1735..2463)
                     /locus_tag="PLO35_09320"
     CDS             complement(1735..2463)
                     /locus_tag="PLO35_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOH79911.1"
                     /translation="MTGERLKAVRKHLNINQQELGEALKINASAISQMEHNHIKPSLD
                     TLVTLSNRYNVDLHWLITGKGDMFMASEDKRSQDATKNLSKLKAFINDELMNMVRVKE
                     SNLQAEAYDLPVMGEIAAGPAVETSDTELDVVSVRRSMIHGAIGDYVCLRVNGHSMEP
                     DIHHNDVVIIRKGNDWDRLKGAICAVRIDGSITLKKLSLDDRRKLILLLPINEDYAPI
                     VIDPRDHEDVSLIGSMHYLYRKIG"
BASE COUNT          680 a          807 c          994 g          878 t
ORIGIN      
        1 ggagagtcga gctcgaggac cggctcggtc ttggagtctg gcagttcctg cactttggag
       61 agctttttct cgatctggcg ggtgcggtct gtggccgttt cgatatgttt ggaggcttcg
      121 tgcagctttt tctgggtctg gtccagcagc gtggcgaatt tgccgaagtc gtgtttgacg
      181 gcggtgagca ctttccaggc ctcggcggtc tttttctgga tggcgagggt gcggaagccc
      241 atctggaggc tgttgaccag cgcggcggtg gtggtggggc cacagattac cactcgttgc
      301 tcgcggatca gcgtttcaaa gagccctgtg atcctcaaag cttcggcata gaggccttca
      361 aagggcaaaa agaggaggcc aaaatcggtg gtgacggggg gattgatata cttgtcgcga
      421 atatctctgg cgcaggcttt gatcctgtcc gccatagctt tacggctgct cacgatctgg
      481 atgggatcat tggcttcata ggcatccagg aggtgctgat agtcctccac cggaaacttg
      541 gaatccatgg gcagatacac ggacttttgg tcttcgtccg gaccgggaag ccggatggca
      601 aattccaccg tttcgttgga gcgggcgcgg ggcttgaagt tggtctcata ctggccggga
      661 agcatgatat cctgcagcag ggtagccagt tgcacctcgc ccatgacgcc acggtttttg
      721 acgtttccca tgatctgttc cagcttgccg acgctcttgg tgaggctttg cacctcgccc
      781 atggtgcgat gcaccttttc cagccggtcg ctgacctggt taaagtgttc tcccagacgc
      841 ttttccagcg tttcgtgcag cttttcgtcc acggtgcggc gcatctcttc cagcttggtc
      901 tcgttgttgg agcggatctg ctccatgcgg gcgtcgagcg tggtcttgag ggtgtcgaag
      961 ctggtctgct gggtggaaga gaggtcctgc acgcggcgcg tgagcgattc ggcaaggttg
     1021 ttgagcgctt tggaaagctc ttcgcggttt gtgaggctgt ctttgttgat ctgctcgcgc
     1081 agggccttga gagtggtgtt cagctcttca cggttgcttc tggtttcgag ggcggagctt
     1141 ttttgcatct cttcaaggcg ctcgctcacc agcttgaagg aatccgtcag ccgcttttcc
     1201 agagtttcgt gcagcttttc atccacggtg cgtcgcatcg cttccagtct ggtttcattg
     1261 ttagcgcgga tctgttccag gcgggtctcg atgctggtcc tcagagtttc cagtccgttg
     1321 tgctgggctt cgctgagctc tttcatgcgg cgggacagtg attccgagag gttgttttgg
     1381 gcgctggtga gctcttcgcg atggccgcgg ttctcgtttt taaggccttc ggagaggctg
     1441 ccgatgtttt ggttcaattc catccgctgt tcggtattga ggcgtcccag ttccgatctg
     1501 aggcccagat tttcttcccg gacctgtttg ccgaggcttt cgagaccggc ggacatgctc
     1561 tcaaaccgct ggctgagagt gtcttctggc ccggaccttc tcagcagcac taacagcagg
     1621 ataatcccca gggccagatt gagagccagc agggctatca tcaacacttc catgcgctta
     1681 tgacctcgct tgtgtagggg tggctattgc tggagggagt tggcgatgca gtcttcagcc
     1741 tatcttgcga tagagataat gcatcgagcc aatcaggctg acgtcttcgt gatcacgcgg
     1801 gtcgatcacg atgggagcgt aatcttcatt gatgggcagc agcaggatga gcttgcggcg
     1861 atcgtccagg ctaagctttt tgagagtgat ggagccatcg atgcggacag cgcagatggc
     1921 acccttgagg cggtcccaat cgttgccttt gcggatgatg acgacgtcgt tgtgatggat
     1981 gtccggctcc atgctgtggc cgttgactct gaggcaaacg tagtctccga tagcgccatg
     2041 gatcatgctg cggcgcacgc tcacgacgtc cagctcggta tcgctggtct cgacggccgg
     2101 tccagccgcg atctcaccca tgacgggaag gtcataggct tcggcctgga ggttgctttc
     2161 cttgacgcgc accatgttca taagctcgtc gttgatgaag gctttgagtt tgctgaggtt
     2221 tttggtggcg tcctggctgc gtttatcctc ggaggccata aacatgtctc ccttaccggt
     2281 gatcaaccag tgcaggtcca cgttgtaacg gttggacagg gtgacaaggg tgtccagtga
     2341 tggtttgatg tgattgtgtt ccatctgaga gatggcggaa gcgttgatct tgagggcttc
     2401 acccagctct tgctgattga tgttgaggtg cttgcgcacg gctttgagtc gttctccggt
     2461 catgattcct ccctgagtgg tatgtattta gcgttgcttt taatgtgtct tagcgttata
     2521 aagtcaaact aatttggaag agcgggcgtg tcaagtcttt tttttatcat tacttcacaa
     2581 aggaatcact ttgcttagtc ctgatcgctt aaaggtgatg aacttacatg catgataaat
     2641 atccgccccg ggtttttcag cttttgcttg cctgttagct gccgtggaga tgccgcgggg
     2701 cctccggcga aaatcgccgg cgggggcacg gcatcgtgac ggcaactaaa gagcgggaaa
     2761 gcagaacctt ctgtttaaaa aacagagaaa cagagggata gaggtttttg aggtacagag
     2821 ggttaggaat tgagaatgga caattgataa ttgagaatgc tctcgctggt ggagccggaa
     2881 acgtcctcgt ttccgggggc ctggatagtc gaattccaat tcggctgcgt gcttcgctta
     2941 acgcataacg tgtaacgata acaagcttca gagaaagcag agaaagcaga gtaactgagg
     3001 aggggattta caatcagaac atgagaggtc tgtcataatc accgtaatgt atccaggcgt
     3061 cagtataacg cataacaggt aacgcgcaac ccaaaaacaa ccagacatga aaatatgaga
     3121 aatccatcat ctgcatcagg tgtaaccatg ggcgccagca taacgcataa cgggtaacgc
     3181 gtaacgcaga aaacaatgag acatgagaaa atgagattct ttaaaacaat gagacatgag
     3241 aaaatgagat tacagaattc atagtagagc cctcacctac cgaagttatc tcacctaccg
     3301 aagttatctc acctaccgaa ggtatctcac ctaccgaagg tatctcacct accgaaggt
//
LOCUS       DAOSFN010000192         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_26812_length_3325_cov_10.030275, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000192 DAOSFN010000000
VERSION     DAOSFN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26812_length_3325_cov_10.030275"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="PLO35_09325"
     CDS             complement(<1..1759)
                     /locus_tag="PLO35_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH79912.1"
                     /translation="MNEIDAPRGSWKNLVQIYRIMFRYWGNMLSGILTMLLFALFSGI
                     SITLVIPLFDYVFAPGKVDIRYHDPSAFVHEVFRLISDFLSSRGGILGISGLKDLSPL
                     WESLQEVMLHTDSLSLLYVLCGFVLITFLLKNIFFFLHKVFFVRLRGNTIRDVRNLMF
                     RKYMGQSLAFFNRNRVGDAIVRMVNDVDIVSEKFIKALLDAVRDLITVFVYMRLAYLL
                     SPTLFVYSLLVLPAFTLSIGYLGKKIKKYSRRIQSRLSTMFSAVEEALNSMKIVKAFR
                     REDSEYRSFSAINRNHLRMWKRAQTYAALNVPISEINSALTGIVVIIIGGKMILAPGS
                     TFSLGDFTAFLFALFSMMHPLKTITQLYGDIKKATVSLDRISLVLNQSSEVKEAPDAI
                     AKQSFNDRITLDKVSFWYRQDKPVLQEISLEIPKGGKIALVGASGGGKTTLANLLNRM
                     YDAREGSIKIDGVDIRQIRLNDLRRLFGVVTQDSVLFTRSIRDNIAFGSLEEVSQARI
                     EQAAQVAYADEFIASFPNAYDEVLDTKGANLSGGQRQRLCIARAIIGDPPILIFDEAT
                     SALDTESERKVQDAIDEATK"
     gene            complement(1809..1885)
                     /locus_tag="PLO35_09330"
     tRNA            complement(1809..1885)
                     /locus_tag="PLO35_09330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1849..1851),aa:Arg,seq:acg)
     gene            1996..2667
                     /locus_tag="PLO35_09335"
     CDS             1996..2667
                     /locus_tag="PLO35_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOH79913.1"
                     /translation="MKITYYGHAAFGFETAAGQHILIDPYLSGNPACPVDPESLTADW
                     IILTHAHGDHIGDALKIADKSLTTFICVTELAKFISDKGFKTHGMQIGGAFRFPFGRL
                     KLTLALHGSLTPDGRYAGLAAGVVLSADGTTIYHCGDTGIFYDMKLIGELDKIDFMLV
                     PIGGNYTMDIPDALKAVELARPSIAIPMHYNTFPIIQADPEIFARGVRTLGLECRILK
                     PGDSV"
     gene            complement(2696..3196)
                     /gene="tpx"
                     /locus_tag="PLO35_09340"
     CDS             complement(2696..3196)
                     /gene="tpx"
                     /locus_tag="PLO35_09340"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOH79914.1"
                     /translation="MAKTKLQGHPINTNGALPRLGCKAKDFVLTAGDLSDKTLADFKG
                     KKILMNIFPSIDTGVCATSVRTFNKEAAKLKNTVVLGISRDLPFAQARFCGAEGIDKV
                     YTLSELRGREFGRAYGLEIVDGPMAGLLARAVIVVDTDGKVVYRELVDDITHEPDYKA
                     ALKALK"
BASE COUNT          769 a          895 c          918 g          743 t
ORIGIN      
        1 ttttggtagc ttcgtcgatg gcgtcctgca ccttacgctc gctttcggta tccagagcgc
       61 tggtggcttc gtcgaagatc aggatgggcg gatcgccgat aatggcgcgg gcgatgcaga
      121 gccgctgtct ctgtccaccc gaaagattgg cgcctttggt atccagcact tcatcgtaag
      181 cattggggaa agaggcgata aactcatcgg cataagccac ttgagcggcc tgttcgatgc
      241 gggcctggct cacctcttcc aaggagccga aagcgatgtt gtctctgatg ctgcgggtga
      301 aaagtacgct gtcctgagtg acgaccccaa agagccggcg cagatcgttg agcctgatct
      361 gccggatatc caccccatct attttgatgc tgccctcgcg cgcgtcatac atacggttca
      421 ggagattggc cagagtggtc ttgccgcctc cgctggcccc cacgagggcg atctttccgc
      481 ccttgggaat ttccaggcta atctcttgca gcacgggctt atcctggcga taccagaagc
      541 tcaccttgtc gagcgttatc ctatcgttaa agctttgctt ggcaatggca tccggcgctt
      601 ccttcacctc agagctctga ttgagcacca gtgagatgcg gtccagggac acggtggcct
      661 ttttgatgtc cccatagagc tgggtgatgg tcttgagggg atgcatcata gaaaagagcg
      721 cgaagagaaa ggcggtgaaa tctcccagac tgaaagtgga gccgggagcc aggatcatct
      781 tgccgccgat gatgatcacc acgatgccgg tgagggcgga attgatctct gagataggaa
      841 cgttgagcgc ggcgtaggtc tgggcccgtt tccacatcct gaggtgattg cggttgatgg
      901 cactgaaaga ccggtattca ctgtcttccc gccggaaggc cttcacgatc ttcatgctgt
      961 tgagcgcttc ctccaccgcg gaaaacatcg tggaaagacg ggactggatc ctgcgggagt
     1021 atttcttgat cttcttgccc agatatccga tcgaaagcgt gaaggccggc agaactaaaa
     1081 gactgtaaac aaagagggta ggactgagca gataggccaa gcgcatatag acaaacacgg
     1141 tgatgagatc acgcacggca tccagcagcg ctttgataaa cttctcgctc acgatgtcca
     1201 cgtcgttcac catcctcacg atggcgtctc ccacgcggtt gcggttgaaa aaggccagtg
     1261 attgccccat atatttgcgg aacatgaggt tgcggacgtc ccggatggtg tttccccgca
     1321 ggcggacaaa gaacacctta tgcaaaaaga aaaagatgtt tttgagcagg aaggtgatca
     1381 acacaaagcc gcagagcaca tagagcaggc tgagggaatc ggtgtgcagc atcacttcct
     1441 gcaggctttc ccagaggggg ctcaggtctt tgaggccgga aatccccaga attcctcccc
     1501 gggagctcag aaaatcagaa atcagcctga agacctcatg cacaaaagcg ctgggatcgt
     1561 gatagcggat atccaccttg ccgggagcga aaacatagtc gaacagaggg ataacaaggg
     1621 tgatgctgat accgctaaaa agggcaaaaa gcagcatggt cagaatgccg gagagcatgt
     1681 ttccccaata tcggaacatg atccggtaga tctggaccag gtttttccag gaaccgcgag
     1741 gggcgtctat ttcgttcatt tgtgtaccgt taaacaaaaa aagccagctt ctgccggcaa
     1801 gttcaatctg gcacgcccta agggagtcga acccctaacc ttctgatccg tagtcagatg
     1861 ctctatccag ttgagctaag ggcgcgcaga gaggttcatc ttttcccagc cccttttttt
     1921 gtcaagccca aagattgagc ttgacccaat cggagtacct gctcacatgg aaacacaaaa
     1981 tacaggagct gtcttatgaa gatcacctac tacggccatg cggcctttgg atttgagacc
     2041 gccgccggcc agcacatcct catcgatccc tacctgagcg gcaatcccgc ctgtccggta
     2101 gatcctgaaa gcctcacggc ggattggatc atcctcaccc acgcccacgg agatcacatt
     2161 ggcgatgccc tcaaaatcgc ggataaatcc ctcaccactt ttatctgtgt gacggaactg
     2221 gccaagttca tatcagacaa gggcttcaag acccacggta tgcagatcgg cggagctttc
     2281 cgctttccct ttggcaggct caaactcacg ctggccctgc atggcagcct gactcccgat
     2341 ggacgttatg ccggactggc cgctggtgtg gtgttaagcg ccgacggcac aactatctac
     2401 cactgcggag acaccggtat tttctatgat atgaagctga tcggcgagct ggacaagata
     2461 gattttatgc tggtgcccat cggcggaaac tacacgatgg atatcccgga tgccctgaaa
     2521 gcggtggaac tggcgcggcc cagcatcgcc atccctatgc actacaacac cttccccatc
     2581 atccaggccg atccggaaat ctttgccaga ggtgtgagaa ccctgggcct ggagtgccgg
     2641 atcctaaaac cgggagacag cgtctgatca caccgcctcc cggaggtggg agaacttatt
     2701 tgagcgcttt gagcgcggct ttgtaatccg gctcgtgggt gatgtcgtcc accagttccc
     2761 gatagacgac cttcccgtcc gtatcgacga ctatgacggc ccgcgccagc aatccggcca
     2821 tcggtccgtc cactatttcc agtccgtagg ccctgccaaa ttcacggccc cggagctcgg
     2881 agagggtata aactttatcg atgccctcgg ccccgcaaaa gcgcgcttgc gcgaagggca
     2941 gatcgcgcga gatgcccagc accacggtgt ttttgagctt ggcagcttct ttattgaagg
     3001 tgcgcacgct ggtggcgcag acacccgtgt cgatgctggg aaagatgttc atcaatatct
     3061 ttttaccctt gaaatcggcc agggtcttat ctgaaagatc ccccgccgtc agcacaaagt
     3121 ctttggcttt gcagcccagg cgcggcaatg ctccattggt gttgatcgga tgcccctgaa
     3181 gtttggtctt ggccatgtta tctcctatga tattggtatt atatcacaca atctaaagct
     3241 gaatccgatt tgagcaagtg aaaaaccgct ggggagagta gaggggcacg gagggaggtt
     3301 ttagggtttt agggttttag ggttt
//
LOCUS       DAOSFN010000193         3274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_27422_length_3274_cov_10.419385, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000193 DAOSFN010000000
VERSION     DAOSFN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27422_length_3274_cov_10.419385"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLO35_09345"
     CDS             complement(<1..305)
                     /locus_tag="PLO35_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79915.1"
                     /translation="MLLPGGRAVKRNQREMQGFRGLGVWGLPKWNVTPVGLEIDTPID
                     LLIASFIKRKLSAVAPARGLAKVLVCLGPELVALQEIERRKREKGDDVDHPVGVQAAT
                     "
     gene            398..1297
                     /locus_tag="PLO35_09350"
     CDS             398..1297
                     /locus_tag="PLO35_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH79916.1"
                     /translation="MKHGFTYLKATLAMLFWAVTFVWFKVAFQLYKPHEIVVMRLLLA
                     SLLLFGVMLISRHWQKISRRDLGRMLLVAFCEPFLYFVGEANGMQYVSSTLGSLVIST
                     IPLVTAFGAWMFLREKITPWLFVGLIVSFAGVAVMSMDSTDLSGTLKGIAFLLLAVFA
                     GMFYGILVKRLTHAYSALTIVAWQSFFGLLFFLPLFLIFDLKHFIGIQHQFLPLAAVI
                     GMSLFASVGAFLLYTGVIRDLGVIRSNVFTNLIPVFTVILAFLLLGDRLGIRSGLGLG
                     LTLLGLFVSQYPDLRQLHKRMMG"
     gene            1375..3261
                     /locus_tag="PLO35_09355"
     CDS             1375..3261
                     /locus_tag="PLO35_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOH79917.1"
                     /translation="MRINTRYLLLAILLLASVMSWADIASPNVPVPVKESVSFAITPK
                     TLKPGERGSISVTLNIPSGKHQSHDPSDPAYFYLEASHPSLKFGKVIYPKAHKVVSAT
                     QWDYHGKVTLRLPFTVKADAKPGKIEIKALLAYNLCFDDGMCEPPEEAEKTLRLEILA
                     LDPNQAPVEAEESQLEASPADSTQAAPVTDPATPPQGGAGGAEKADAGKMSDILKYMI
                     FALLGGLILNITPCVLPILPIRVMAIMNQAHQDRAKVLLHTLVYALGVMISFGILAGV
                     FIGLRSAGEQQGWGFQNQNPMFTVVLMSVVFSFALSLLGVFVLTAPGMNTASKATAKR
                     GYSGSFFGGIFAFLMAISCTGPFLGAALPFAFKLSSPLLMLFFLIIGLGFALPFILIG
                     MIPSALKLIPKPGEWMNIFKEVMGFVLLYIVYTMLKTTLALTSGAYLLNVIFFLILLG
                     FALWLYGRFVRNENSSATQWIFSFLAIAVIVASAWYFLPLKPEYLEAETVQVANADGL
                     TPAPHAPEGWYVFSPELHKKLLDEGKPVFLDIGADWCKNCLTNEKTVLFTAAVMQDFK
                     AKGVTLLRGDFTRKDEVLLKWIQDHDRAGVPFNALYIPGKEPILFPELISAKAVREAL
                     AQIP"
BASE COUNT          635 a          942 c          888 g          809 t
ORIGIN      
        1 gtggccgctt gaactccaac ggggtgatct acatcatccc ctttttccct ttttctgcgt
       61 tctatttctt gtaaggctac aagctccggg ccaaggcaaa cgaggacctt tgccaatcct
      121 ctggctggcg cgacagcaga gagttttctc tttataaaag aggcaatgag caaatcgatc
      181 ggagtgtcga tttccaaacc gacaggtgtt acgttccatt ttggtaaccc ccaaacccct
      241 aaacccctaa aaccctgcat ctcccgctga tttcgcttga cagctctgcc accgggtaaa
      301 agcatgcccg ttcaacgcca ttcttatcaa gatggcctta actgtatcca ggagaaagac
      361 aaacgatgtt atccacccca gccggtcctg tccgtccatg aaacacggct tcacctatct
      421 gaaagccact ctggccatgc tcttttgggc ggtcaccttt gtgtggttca aggtggcgtt
      481 tcagctatac aagccccacg agattgtggt gatgaggctg ctattggcct cgctgctgtt
      541 gtttggcgtg atgctgatca gccggcactg gcaaaagatc tcgcggcggg acctgggcag
      601 gatgttgctg gtggcctttt gcgagccctt cctctacttt gtgggcgagg ccaacgggat
      661 gcaatatgtg tcttccactc tgggttcgct ggtgatctcc accatccctt tggtgacggc
      721 tttcggggcc tggatgttcc tgcgggaaaa gatcacgccc tggctgtttg tgggcctgat
      781 cgtctccttt gccggagtgg cggtgatgag catggattcc accgacctca gcggcaccct
      841 gaaggggatc gcgtttttgc tgctggccgt gtttgccggg atgttttatg gcatcctggt
      901 caagcggctc acgcatgctt attcggcctt gacgatcgtg gcctggcaga gcttttttgg
      961 gctgctgttt ttcctgccgc tgtttttgat ctttgatctc aaacacttta tcggcattca
     1021 acatcagttt ttgcccctgg cggccgtgat cgggatgagc ctcttcgcct cggtgggagc
     1081 tttcctgctc tacactggcg tgatccgcga tctgggagtg atccgctcca acgtgttcac
     1141 aaacctgatt cccgtcttta cggtgatttt ggccttcctg ctgctgggag accggctggg
     1201 catcaggagc ggactgggac tggggctcac gctgctggga ctgtttgtct cgcagtatcc
     1261 cgatctgcgg cagcttcata agcggatgat gggctgaaag atgcttgaca tgtggaacat
     1321 atcgtgcatt atatcaatat atcataaagt atttagcgtg gaggtccaag ctatatgcgc
     1381 ataaacacca gatatctgct gctggctatc ttgttgctgg ccagtgtgat gagctgggcg
     1441 gacatcgcct cccccaatgt tccggtgccg gtgaaggaaa gcgtgagctt tgccatcact
     1501 cccaagaccc tcaagcctgg agaacggggc agcatcagcg tcaccctcaa tatcccttcc
     1561 ggcaagcatc agagccacga tccctcagac ccggcctact tctatctgga ggccagccat
     1621 cccagcctca aatttggcaa agtgatctat cccaaggccc acaaggtggt gtctgccacc
     1681 caatgggact accatggcaa ggtcacgctg cggctgccct tcaccgtgaa ggcggacgcc
     1741 aaacccggca agatcgagat caaggccctg ctggcctata acctctgttt tgacgacggc
     1801 atgtgcgagc ctcccgaaga ggcggaaaag accctcaggc tggaaatcct ggccctggac
     1861 cccaatcagg ccccggtgga agctgaagaa tctcaactgg aagccagccc agcggacagc
     1921 actcaggccg cccctgttac cgatcccgcc actcctcctc agggaggagc gggtggggcg
     1981 gaaaaagctg acgccggcaa gatgtctgac attctcaagt atatgatctt cgctctcctg
     2041 ggcggtttga tcctcaatat cactccctgc gtgctgccga tcctgcccat ccgggtgatg
     2101 gcgattatga accaggcgca tcaggaccgg gctaaagtgc tgctgcacac gctggtttac
     2161 gctctgggag tgatgatctc ctttggcatt ctggcaggag tgtttatcgg tctgcgttcg
     2221 gctggagaac agcagggctg gggcttccaa aaccagaacc ccatgttcac ggtagtgctg
     2281 atgagcgtgg tgttttcctt cgcgctgtcg ttgctgggcg tgtttgtgct caccgctccg
     2341 gggatgaata cagcctcaaa ggccactgcc aagcggggct acagcggttc ctttttcggc
     2401 ggcatctttg cctttttgat ggcgatctcc tgcacgggtc cttttctggg agcggcgctg
     2461 ccctttgcct tcaagctttc ctctccgctt ctgatgctgt ttttcctcat catcggtctc
     2521 ggctttgccc tgccctttat cctgatcggc atgattcctt ccgccctcaa gctcatcccc
     2581 aaacccggcg aatggatgaa tatcttcaag gaagtgatgg gctttgtgct gctctacatc
     2641 gtgtatacca tgctcaagac caccctcgct ctcaccagcg gggcctatct gctcaacgtg
     2701 atcttcttcc tcatcctgct gggcttcgcg ctctggctct atggccgctt tgtgagaaac
     2761 gaaaacagca gcgccacgca gtggatcttc agcttcctgg ccattgccgt gatcgtggcc
     2821 tcggcctggt atttcctgcc tctcaagccg gaatatctgg aggccgagac ggtccaggtg
     2881 gccaatgccg atggcctcac tcccgctccc cacgctcccg aaggctggta tgtcttcagt
     2941 cccgaactgc acaaaaagct gctggatgaa ggcaagccgg tgtttctgga tatcggggcg
     3001 gactggtgta aaaactgcct gaccaatgaa aagaccgtcc tcttcaccgc ggcggttatg
     3061 caggatttca aggccaaagg agtaacactt ctgaggggag atttcacccg caaggatgag
     3121 gtgctgctca aatggatcca ggaccacgac cgggccggag tgccattcaa cgcgctctac
     3181 atccctggta aagagccgat cctgtttccc gagctgatct cggccaaagc ggtgcgggaa
     3241 gccctggcac agataccttg agacgatgag agct
//
LOCUS       DAOSFN010000194         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_27464_length_3271_cov_7.759328, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000194 DAOSFN010000000
VERSION     DAOSFN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27464_length_3271_cov_7.759328"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..811
                     /locus_tag="PLO35_09360"
     CDS             251..811
                     /locus_tag="PLO35_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79918.1"
                     /translation="MRKYAVLLIALLALCACELFEVRDSEPPTKPPSWNNFTTSWELC
                     LQNLSYCYEDARNVVKYGGLFTEGFRFSFAPQDINDYGISGIWGRAQEQDMLLSLHGQ
                     CDSLQLDLQPISGQNDDISASEAIIYRSYELIAFKKDRATPEPYSGNLELRFRKDGGY
                     WYIDRWYDYRGATPPTWGKLKYDNSQ"
     gene            795..1382
                     /locus_tag="PLO35_09365"
     CDS             795..1382
                     /locus_tag="PLO35_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79919.1"
                     /translation="MITRNRTSILVLLLLSLLLQSCWNPFRPRMIDNSNDLIHNRTPL
                     ELLQNLERAYRERNINIYKSLLAPDFRFELISSEVNLIGIDVNEDGIRDSWWGFDEDV
                     EFTDNLFNHGSTDGVYPSPDQITLRLMVPPEENWETDPELGHEDWIVIACNFDLTLTY
                     LSSNSSLAAGGVARFYLRPSGNRWYIAIWRDESNL"
     gene            1379..1792
                     /gene="ruvX"
                     /locus_tag="PLO35_09370"
     CDS             1379..1792
                     /gene="ruvX"
                     /locus_tag="PLO35_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOH79920.1"
                     /translation="MSRILAIDHGTVRIGLAISDPLRIFAKPLKVIPNQGFRAVSTQI
                     LDLIKDQEVGMVLLGLPLAIDGSKTPKTLEVEKFGEKLMQLLEVELIFWDERYSSSEA
                     EAELRKLGYSWQESRSLRDAMAAAMILKNYLEHNA"
     gene            1789..2781
                     /gene="mltG"
                     /locus_tag="PLO35_09375"
     CDS             1789..2781
                     /gene="mltG"
                     /locus_tag="PLO35_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOH79921.1"
                     /translation="MKALKYILILGLALLLALGGYLAWQILMPLDSQQIVLRVESGDN
                     ARTIAQRLAQNKIIRSRELFLLLAKWRGTDRKLQAGAYSFGGHYNLNQTLRLLEDGNT
                     EAIRITIPPGFSMFQVLRRIERSGLASYDSLYARATDPAFVNQLTGFALPSLEGWLFP
                     DTYSYDIATPVDTLLALPVRNFLRKAKVAGLDPQATPDFQSKLTLASIVEKESAHPDE
                     RVIIAGVYLNRLRKGMRLESCPTVDYILERRGISRTVLLNSDLAIENPYNTYRNDGLP
                     PGPICNPSLESLQAVINPQPNNFLFFVSNRKGRNDFSATYQEHLSKKRKYERGG"
     gene            complement(3076..3150)
                     /locus_tag="PLO35_09380"
     tRNA            complement(3076..3150)
                     /locus_tag="PLO35_09380"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3116..3118),aa:Gln,seq:ctg)
BASE COUNT          812 a          924 c          818 g          717 t
ORIGIN      
        1 cagtatgcca agatgactcc agaagaaata tccaggattc acgagaattt tccagacatc
       61 aaatggaaaa cgcatcctgc taaacgagct aaatcagtac gggctgctaa actcagacac
      121 taaagtaaaa ccactaatag ggccagagat attgctaagc ttagccagcc tggtgatatc
      181 tgtattgttc aggtaatctt gccgtcaact ttgtagttga cacctttaca gactccgcca
      241 ttatggcaat atgcgaaaat atgccgtgct cctgatagcc ttgctggcgc tgtgtgcctg
      301 cgagctcttt gaggtgaggg actccgagcc ccccaccaaa ccgccctcgt ggaataattt
      361 caccacgagc tgggagctgt gtctgcaaaa tctgagctat tgctatgagg acgcccgcaa
      421 tgtggtcaag tatggcggtc tcttcacgga aggtttccgc ttcagctttg ctccgcagga
      481 catcaacgac tacggcatca gcggcatttg gggaagggct caggaacagg atatgctgct
      541 ctccctgcac ggccagtgcg acagcctgca actggatttg cagcccatca gtggtcaaaa
      601 tgacgacatc agcgcctctg aagcgatcat ctaccgctcc tacgaactta tcgcttttaa
      661 aaaggacaga gcgaccccgg aaccctattc cggcaatctg gaattgcgct ttcgcaaaga
      721 cggaggctat tggtatatag acagatggta tgactaccgc ggagctacac cgcccacctg
      781 gggtaaactg aagtatgata actcgcaata gaacctctat ccttgtgctt ctgctcctca
      841 gcctgctgct gcaaagctgc tggaacccct tccgccccag gatgatagac aactccaacg
      901 acctcatcca caaccgcact cctctggaac tgctgcaaaa cctggaacgc gcctatcgcg
      961 aacgtaatat aaatatctat aagagcttac tggcaccgga ctttagattt gaactgatct
     1021 cctcagaggt caacctgatc ggcatcgacg tcaatgaaga cggtatccgc gattcctggt
     1081 ggggttttga cgaagacgtg gagtttacag acaacctctt caatcacggc tccacggatg
     1141 gagtgtatcc ctcgccggac cagatcactc tgcgtctgat ggtcccgcct gaagagaact
     1201 gggaaaccga tcccgagctc ggacatgaag actggatcgt gatcgcctgc aattttgacc
     1261 tcacgctcac ttatctgagt tccaacagca gcctcgccgc gggtggagtg gccagattct
     1321 atctccggcc ttccggaaac cgctggtata tagccatctg gagggatgaa tccaatctgt
     1381 gagcaggatc ctggccatcg atcacggcac tgtgaggatc gggctggcaa tctcagaccc
     1441 cctgcgcatt tttgccaaac cgctcaaagt gatccccaat cagggcttca gggctgtcag
     1501 cacccagatt ctggacctga tcaaggacca ggaagtggga atggtgcttc tgggcttacc
     1561 gctcgccatt gacgggtcca agactcccaa gaccctggaa gtggagaagt ttggtgagaa
     1621 actgatgcag ctgctggagg tggaattgat cttttgggat gagcgctact ccagctcaga
     1681 agctgaggct gaactacgca aactcggata ctcctggcag gaaagcagaa gcctgcggga
     1741 cgccatggcc gctgccatga tccttaaaaa ctatctggag cacaacgcat gaaagcactt
     1801 aaatacatac tgatattagg gttggcacta ctgctcgcct tgggtggata ccttgcctgg
     1861 cagattctga tgccgctcga ttcccaacag atcgtgctca gagtcgaaag cggagacaac
     1921 gccaggacga ttgcccaacg gctggcccaa aacaagatta tccgcagccg cgagctcttt
     1981 ctgctgctgg caaaatggcg gggcaccgat cgcaaactac aagccggagc ttatagtttt
     2041 ggcggacatt acaacctcaa tcagaccctc agactgctgg aagatggcaa taccgaagcc
     2101 atcaggatca ccatcccacc cggtttttcc atgtttcagg tgctcaggag gatcgaacgc
     2161 agcggtctgg cctcctacga cagcctctat gccagggcca ccgatccggc ctttgtgaat
     2221 caactcacag gttttgcctt gcccagcctg gagggctggc ttttccctga cacctattcc
     2281 tatgatattg ccactccggt tgatacgcta ctggctctgc cggtccggaa cttcctcaga
     2341 aaggccaaag ttgcgggtct tgatccccaa gcgactccag atttccaatc caaactcacc
     2401 ctggcctcga tcgtggaaaa agaaagcgct catcccgatg agcgagtcat cattgccggt
     2461 gtctatctga accgcctgcg taaaggcatg cggctggaaa gctgtcccac cgtggattat
     2521 attctggaac ggcgcgggat cagcaggacc gtcctgctca acagcgatct ggccatcgag
     2581 aatccctaca acacctaccg caacgatggc ctccccccag gccccatctg caatccctct
     2641 ctggaatcgc tgcaggcagt gatcaatccc cagcccaaca acttcctctt ttttgtatcc
     2701 aaccgcaagg gccgcaacga tttctctgcc acttaccagg agcacctgag caagaaacgc
     2761 aagtacgagc gcgggggatg atcctgcctc tcccatggta ccgcaaacgt ccacgtctgc
     2821 gtttccgtat cggccaattc caattcggcg cgtgtgttaa ctactgagat acagagtttt
     2881 ccttctttca ctacagagct actgagggaa ccgagagata tacagagaaa ggggttggat
     2941 atcaaaacag atgatcccct ggggcaaaca catatttatg gagcagcaga cgtccacgtc
     3001 tgcgtctttt agcatctacc caatgaattc agcccataaa tcaaaaagcc tccgttaagg
     3061 aggcttttat ataactggct gggtagggag gattcgaacc cccggatggc agggccagaa
     3121 cctgctgcct taccgcttgg cgactaccca gcaaaaatct atctgaacgc ttggtggaaa
     3181 ggagcggtga gcatcctttg ccccagccgg ttaagctggc tgggcaggga ggattcgaac
     3241 ccccggatgg cgggaccaaa acccgctgcc t
//
LOCUS       DAOSFN010000195         3261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_27573_length_3261_cov_8.999376, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000195 DAOSFN010000000
VERSION     DAOSFN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27573_length_3261_cov_8.999376"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..1513)
                     /gene="glmM"
                     /locus_tag="PLO35_09385"
     CDS             complement(158..1513)
                     /gene="glmM"
                     /locus_tag="PLO35_09385"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOH79922.1"
                     /translation="MSKLMTGVSGVRGVFADTLDPFVATRFAARFGMLVKGDLDSATV
                     VVGRDSRTTGSVMLNAVLAGLTGVGCNVIEIGIVATPTVLLNVQRFGAQGGIAITASH
                     NPPEWNAMKFVDCDGMFLSPDRAAEFLGSVEEPIAWQDWQQIGRVEHRDDAVKHHIDK
                     VLAIPYLDIEAIRSRRIKVVLDSVNGAGGLISPLLLNELGCELVSINEAPTGVFAHPA
                     EPLNHNLTQLEEAVKLHKADIGFATDPDVDRLSIVNEQGECIGEELSVVLAQLFVLPK
                     NKGDIVVNLSTSMLSDDVARRFGVKVHRAKVGEINVGKKMRELRSPIGGEGNGGIICP
                     DVNYTRDAIAGMALILGLLAESGTSVSELRATLPHYYFAKGKLEVEASRLDSIMATAP
                     TLFSGMEIDTQDGIKATASDHWIHIRKSGTEPIIRVYVESGDPQRSEQICYETMNKLK
                     G"
     gene            complement(1520..3250)
                     /locus_tag="PLO35_09390"
     CDS             complement(1520..3250)
                     /locus_tag="PLO35_09390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH79923.1"
                     /translation="MLTGAGSTIFGLREGFFSRKNYVGKINGDKITPQEFQAKLEEVM
                     QRYREQGQEIDENSRRYLASSAWDELVAEKLWQQQVKKHRIKVSDDEILTEIQNNPPQ
                     ELMQNEQLQTNGAFDKKKYLDALKNDVQFYAVMENYMRNYLPRKKLQDKIKKEAGVTM
                     DSLKAEYSKETNLINGKALWFDFNKADSVVVTDAEVRSHYDKNKEKDYKKGPASKIKY
                     LLFEIKPSETDYSLVKSDIDLVLQRLNKGENFATLAETYSEDPGSAKNGGSLGEFGKG
                     QMVPEFEKVAFALKVGEISKPVRTDYGWHIIRCDSIAAVGAEPKIKASHILMSVKPSD
                     TTKNDIMAKAERSRELIAKKGIDAAAKELKVEVMNGQWQPHDQENINGIGNLTALRQF
                     MIKKKEKSVSPVFTDQNGRYIVAYLTDNKKVYYEDFESVKLQIKFQLEKDKKIASVKK
                     KAEAFLARVQPANYFSAGPAEGWKLVELKGHKHDSMISEVNAKSEEFTKAALALKAGE
                     YSKLVMTKEGPFVIWAETRQMPDLAAFAKDKAKQKEIRDRLEDAAFNRWFQQLKKDAK
                     IVDNRAQFGY"
BASE COUNT          608 a          829 c          985 g          839 t
ORIGIN      
        1 ttgtaactct gtagtgaaaa aagaaatcct ctgtagctca gtagtttaaa cacacgcagg
       61 actggaatcc gagggtatgg gacgcgctga tatgttcaac gctcacaaaa aaagcgggag
      121 ttctgctccc gcctgaatag tctgatatag tatggattta gcctttgagc ttgttcatag
      181 tctcataaca aatctgctcg gagcgttggg gatcgcctga ttccacataa acgcggatga
      241 tgggctcggt gccgctcttg cggatgtgaa tccagtggtc ggaggcggtg gccttgatgc
      301 cgtcctgggt gtctatctcc atgccggaga agagggtagg ggcggtggcc atgatggaat
      361 ccagccggga ggcttccacc tcgagcttgc ctttggcaaa gtagtagtga ggcagagtgg
      421 cgcgcagttc ggaaacgctg gttccgctct cggctaaaag ccccaggatg agggccatgc
      481 cggcaatggc atcacgggtg taattgacgt ccggacagat gattccgccg ttgccttcac
      541 cgccgatggg ggagcgcagt tcacgcattt ttttgcccac gttgatctcg ccgactttgg
      601 cgcggtggac cttgacgccg aagcgtctgg ccacgtcgtc ggaaagcatg gaggtgctga
      661 ggttgaccac gatgtcgccc ttgtttttgg gcagcacaaa gagctgggcc agcaccacgc
      721 tgagttcctc gccgatgcac tcgccttgtt cgttgacgat ggaaagcctg tccacatcgg
      781 gatcggtggc aaaaccgatg tccgccttgt ggagtttgac ggcttcctcg agctgggtga
      841 gattgtggtt gagcggctcg gcgggatggg caaagacgcc cgtgggcgct tcattgatgg
      901 agacaagctc acagccaagt tcattcaaaa gcagggggga gatcaggcct ccggcgccgt
      961 tgacgctgtc cagcactacc ttgatccgac gggagcggat ggcttcaata tccagatagg
     1021 ggatggccaa caccttgtct atatgatgct tcacggcgtc gtcgcgatgt tccacccggc
     1081 caatctgctg ccaatcctgc caggcgatgg gttcttccac ggagccgaga aattcagcag
     1141 cccggtcggg actgagaaac atgccgtcgc agtccacaaa cttcatggcg ttccactcgg
     1201 gagggttgtg ggaggcggtg atggcgatgc cgccctgagc gccaaagcgt tggacgttga
     1261 gcagcacggt gggggtagcc acgatgccga tctcgatcac gttgcagccc actccggtga
     1321 ggccagccag cacggcattg agcatcacgc ttccggtggt gcggctgtca cggcctacga
     1381 cgacggtggc ggaatccaga tcgcccttga cgagcatgcc aaagcgggcg gcaaaacgag
     1441 tggccacaaa aggatcaagg gtgtccgcga agacacccct gacaccggaa actccggtca
     1501 ttaacttgct catagtctat cagtagccaa actgggcgcg gttgtccacg attttggcgt
     1561 cttttttcag ctgttgaaac cagcggttga aggccgcgtc ttccaggcgg tcccggattt
     1621 ctttctgttt ggccttgtct ttggcgaagg ctgccagatc gggcatttga cgggtttcag
     1681 cccagatcac aaagggacct tccttggtca tgacgagttt gctgtattca ccggctttga
     1741 gggcgagggc ggctttggtg aattcttcgc tcttggcgtt gacttcagag atcatgctgt
     1801 cgtgcttgtg gcctttgagc tccaccagtt tccagccttc ggcgggaccg gcgctgaagt
     1861 agttggcggg ctgcacgcgg gccaggaagg cttccgcttt ctttttgacg gaggcgatct
     1921 tcttgtcttt ttcgagctgg aacttgatct ggagcttgac gctttcaaaa tcttcgtaat
     1981 agactttctt gttgtccgta aggtaggcca cgatatagcg gccgttctgg tctgtaaaga
     2041 cgggggagac ggacttctct ttctttttga tcataaactg gcggagggcg gtcaggttgc
     2101 caatcccgtt gatgttttcc tgatcgtgag gctgccactg gccgttcatg acttcgacct
     2161 tgagctcctt ggcggcggca tcgatgcctt ttttggcgat gagctcgcga gagcgttcgg
     2221 ccttggccat gatgtcgttt ttggtggtgt ctgagggttt gacgctcatc aggatgtggg
     2281 aggccttgat cttgggctcg gccccgacag cggcgatgga atcgcagcgg atgatgtgcc
     2341 agccataatc ggtgcggacg ggtttggaga tttctcccac cttgagggcg aaagccactt
     2401 tctcaaattc cggcaccatc tggcctttgc caaattctcc cagggaacca ccgttcttgg
     2461 cggaaccggg atcttctgaa taggtctcgg ccagggtagc gaagttttca cccttgttga
     2521 ggcgctgcag gacgaggtcg atatcggatt tcacgaggct gtagtcggtc tcgctgggtt
     2581 taatctcaaa caagagatac ttgattttgg aggcgggacc tttcttgtag tccttttcct
     2641 tgtttttgtc atagtgagag cgcacttcgg catcggtcac gaccacggaa tcggctttgt
     2701 tgaagtcaaa ccacagggcc ttaccgttga tcaggttggt ttccttgctg tattcagcct
     2761 tgaggctgtc catggtgacg ccggcctctt tcttgatctt gtcctgcaat ttcttgcggg
     2821 gcagatagtt gcgcatatag ttttccatca cggcatagaa ctggacgtcg tttttgaggg
     2881 cgtcgagata cttctttttg tcgaaggcgc cgttggtctg cagttgttcg ttctgcatca
     2941 gttcctgagg aggattgttc tggatctcgg tgaggatttc gtcgtcgctc actttgatgc
     3001 ggtgtttttt gacctgctgc tgccagagct tttcagccac cagctcgtcc caggcggaac
     3061 ttgccaggta acggcgggag ttttcgtcga tctcctgtcc ctgttcgcgg tagcgctgca
     3121 tgacctcttc aagcttggct tgaaattcct gaggggtgat cttgtcgcca ttgatcttgc
     3181 cgacgtagtt tttacggctg aagaatcctt ctctgaggcc aaagatcgtg cttccggccc
     3241 ctgtcaggat aaaggccgct g
//
LOCUS       DAOSFN010000196         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_28621_length_3179_cov_7.755762, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000196 DAOSFN010000000
VERSION     DAOSFN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28621_length_3179_cov_7.755762"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1688)
                     /gene="gcvPB"
                     /locus_tag="PLO35_09395"
     CDS             complement(234..1688)
                     /gene="gcvPB"
                     /locus_tag="PLO35_09395"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOH79924.1"
                     /translation="MSRTIFEHSSPGRKGCTLPPREVDLPLDSIIPAEYRREAIPRLP
                     ELSELDVMRHYIKLSHKNHFIEKGLYPLGSCTMKYNPKVHEALVRYSCFNNVHPYQPE
                     RTLQGSLEILYELQKDLSEISGMAQVTLQPVAGAQGEFTGMKVIAAYHKAKGNTHKTK
                     IIVPDSAHGTNPATCSLVGFEVVELKSNAQGRCDLDHLRSIVDDSTAGFMLTNPNTLG
                     LFETQIEEIAEIIHSVDGLIYMDGANLNALLGIVQPGKIGFDVMHFNLHKTFTTPHGG
                     GGPGCGPVGVVEKLVPYLPVPLVNKNDDGYFFDYGHEATSIGKVHTFYGNFAIMLRAY
                     IYIKMLGAEGLRKVSENAILNANYIMARVKDHYHIQHQEYCMHEFVADSSWQKKANGV
                     NTLDIAKRLLDKGFHAPTIYFPLIVPEAMMVEPTETEPLESLDAFADAMIEIAQECSQ
                     NPELLHEAPLTTPVRRVDDVRAVKMLDPIFKIEK"
     gene            complement(1681..3027)
                     /gene="gcvPA"
                     /locus_tag="PLO35_09400"
     CDS             complement(1681..3027)
                     /gene="gcvPA"
                     /locus_tag="PLO35_09400"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOH79925.1"
                     /translation="MPYISNTDAERQAMLKKIGVSSFEELIAAIPPKMRMMSPLKLDE
                     PLSELEITRKIKSQTCGDLCTSTANAFLGAGVYDHFIPAAVDTIVSRPEFFTAYTPYQ
                     AEVSQGTLQFIYEYQTMICELTGMEIANASMYDGASAIAEAILMAVRKNRLTKAILPA
                     TLHPEYVKVIRIYTEGLGIELLTAPMKDGLTDLEALKTMMDETIGSVVLQNPNFFGNI
                     EAGEAIGQIAHSNPKTLFIVAADPISLAILKAPAAYGADIAVGEGQALGNSMYMGGPL
                     FGYFSTKIDLARQMPGRIVGGTLDIEGKRAYALTLQAREQHIRREKATSNICSNQSLC
                     TLAATVYMALMGREGLVEVATQSAQKAHYLASEIRKLTGFELAYPNAPFFREFVVNTP
                     VPAAQIVEKLLPRAIYPGVDMARFGNEKQLMIAVTEKKNKGDLDALVNALREVTHV"
BASE COUNT          681 a          810 c          909 g          779 t
ORIGIN      
        1 agcagcctgt cggtgtccac ataacccagt tttaccgcct gggcgatgcc aatcgtcact
       61 atggccagca ttgctaatgc cagtatgaat cttttcatta tatctccttg tttggttgtg
      121 cgttacgcgt taagcgttac gcgaggtagc gcccaatcgg gattaggtgc catagtttta
      181 tgcatgagta caacgcgtaa cgcatatcgc ataacgagtg atactgaatc agactatttc
      241 tcaatcttga atatggggtc cagcatcttc accgcgcgca cgtcatctac tctgcgcacc
      301 ggtgtggtaa gcggggcctc atgtaataat tccgggtttt gtgagcattc ctgcgcgatt
      361 tctatcatgg catcggcaaa ggcatccaga gattccagcg gttccgtctc ggtgggctcc
      421 accatcatcg cttcgggaac gatcagcggg aaatagatgg tgggggcatg gaagccttta
      481 tccaaaagcc gcttggcgat gtcaagagta ttcactccat tggctttctt ctgccagctg
      541 gaatccgcca caaactcgtg catacagtat tcctgatgct ggatgtggta gtggtctttc
      601 actcttgcca tgatgtagtt ggcgttgagg atggcgtttt cgctcacttt gcgcagtccc
      661 tcggcaccca gcatcttgat atagatgtag gccctcagca tgatggcgaa gttgccgtag
      721 aaggtgtgca ccttgccaat cgacgtagcc tcgtgaccgt agtcaaagaa gtaaccgtcg
      781 tcgttcttat tgacaagtgg cactggcaga tagggcacca gtttctccac cacgcccacg
      841 ggtccgcaac cgggaccgcc tccaccgtga ggggtggtga aggttttgtg gaggttgaag
      901 tgcatcacgt caaagccgat cttgccgggc tggacaatac ccaggagcgc gttgagattg
      961 gcgccgtcca tataaatgag gccatcgacg ctgtggatga tctcggcgat ctcctcgatc
     1021 tgagtctcaa acaggcctaa agtattggga ttggtgagca taaagcccgc ggtgctgtca
     1081 tccacgatgc ttctcagatg gtccagatcg cagcggccct gagcattgga cttcagttcc
     1141 accacttcaa agcccaccag agaacaggtg gcgggattgg tgccgtgagc gctgtccgga
     1201 acgatgatct tggtcttgtg ggtgttgccc ttggctttgt gataggcggc gatgaccttc
     1261 atacctgtaa actcaccctg agcacctgcc acgggctgca atgtcacctg ggccattccg
     1321 gaaatctcgc tgaggtcttt ctgcagctca tagaggatct ccagagagcc ctgcaaagtc
     1381 cgttccggtt ggtaaggatg cacgttgtta aaacaggaat agcgcaccaa agcctcatgg
     1441 accttggggt tatatttcat cgtgcaggac cctagcgggt agagaccttt ttcgatgaag
     1501 tggttcttgt gagaaagctt gatatagtga cgcatcacgt ccagttcgct gagctcgggc
     1561 agacgtggaa tcgcctctct gcgatactca gccgggatga tgctatccag cggaaggtca
     1621 acttcccggg gaggaagggt gcagcctttg cggccggggg acgagtgttc aaatatggtc
     1681 ctagacatgg gtcacctccc tcagtgcgtt caccagcgcg tcgagatcgc ccttgttctt
     1741 cttctctgtg acagcgatca tgagctgttt ctcattgcca aatctcgcca tgtctacccc
     1801 gggatagatg gcacggggca gcagcttttc cactatctga gccgctggaa cgggtgtatt
     1861 gaccacaaac tcacggaaga agggcgcgtt tggataggca agctcaaagc cggtaagttt
     1921 tctgatctca gaggccagat aatgagcttt ctgagcgctc tgggtggcca cttccaccaa
     1981 accttcacgg cccatgaggg ccatatagac ggtggcggcg agggtgcaga ggctttgatt
     2041 tgagcagata ttggaggtgg ctttttcgcg gcggatatgc tgttcgcggg cctgcagagt
     2101 gagcgcgtag gctcttttgc cttctatatc cagagttccg cccacaatgc ggcctggcat
     2161 ctgacgggca agatctatct tggtgctgaa atagccaaac agcggaccgc ccatatacat
     2221 gctgtttccc agggcctgac cttcgcccac agcgatgtcg gctccataag cagcgggagc
     2281 tttcaggatg gccagcgaga tgggatcggc ggctacgata aacagggtct tggggttgga
     2341 atgagctatc tggccgatcg cctctccagc ctcgatattg ccaaagaaat tgggattttg
     2401 cagcaccacg gagccgatcg tctcgtccat catggttttg agggcttcca gatcggtgag
     2461 gccgtctttc atcggagcgg taagcaattc tatgcccaaa ccttccgtgt agatgcggat
     2521 cactttcaca tattcgggat gcagagtggc gggcaggatg gccttggtga gccggttttt
     2581 gcgcacggcc atcaggatcg cttcggcgat ggcggaagct ccgtcataca tggaagcgtt
     2641 ggcaatctcc atgccggtga gctcgcagat catcgtctga tactcataga tgaactgcag
     2701 agtgccctgg ctgacttctg cctggtaggg agtgtaggct gtgaaaaact ccggacgcga
     2761 aacgatcgta tccacggcgg cgggaataaa gtggtcatag accccggccc ccaaaaaagc
     2821 attggcggtg gaggtgcaaa ggtcaccgca ggtctgggat ttgatcttgc gggtgatctc
     2881 cagttcggaa agcggctcgt ccagcttgag cggactcatc atacgcatct tgggtgggat
     2941 ggcggcgata agctcttcaa aagacgacac accgatcttt ttgagcatgg cctgcctttc
     3001 cgcatccgtg tttgaaatat acggcattag ggctccttaa aaggtgctgc gcacacgcat
     3061 aatattattt tcccaattct aaaatagggg ctgtttgtgt caattgataa attggccccg
     3121 ccagcgaggg gcttttgtga cggcacaagc agcctgcagt ggtgtctgaa gcgctgctg
//
LOCUS       DAOSFN010000197         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_28634_length_3178_cov_9.803714, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000197 DAOSFN010000000
VERSION     DAOSFN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28634_length_3178_cov_9.803714"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1965)
                     /locus_tag="PLO35_09405"
     CDS             complement(244..1965)
                     /locus_tag="PLO35_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79926.1"
                     /translation="MKRALSSGLKVLALMTLLTGLASLAAIPVPYTQNFDLYLPPNLP
                     DGWIAVETGESNSLAQTQAENSHSAPNHIILFEGPSDDYVLLALPPVDETVPVRHLSV
                     WWWARSYQDESELQVGILQDPADPSSFFSLTTDIPLTSYWKRYRLSLACYPSTMGRRV
                     AFKSADPNYGGILIDDVELSYNYNTDLEVTHLEVWDPYYVGQNQDYTARVVNQGIQDV
                     YSYSCQLLDGSGNILAQGNQGALWAGFLGGTGFDYNITTPGNHKLSAKVVCAGDLDPS
                     NDESPSRIVKVLAEGAESFGIQNYMQYTGKYPIDLYWKTSMCETLYLASELPQQDKLI
                     HGVKYLSEINTPDIGIKPIQIWMGITTLENLSAGWIPASEMQLVFDGETDFTPGTSPG
                     MFCFPSPFIYTAGSNLAILVLRPLDSQYYSSTDDFYHDNTSIIRTRNVKSDTAIYDPY
                     DPPVASEALTARPATKLYVTDPITAVDDPVAPALTSLIAWPNPFKQSVHLSWNLKRTA
                     PPELKVYNLKGQMVYRTQIKAEPGQNQSCTWDALDSSGNKLPSGIYLLKLSAGQDSET
                     RKVMLVR"
     gene            complement(2219..3112)
                     /locus_tag="PLO35_09410"
     CDS             complement(2219..3112)
                     /locus_tag="PLO35_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOH79927.1"
                     /translation="MDMKTFEYLYYKARDYSREDILEKARLSEEEYDFLESTLSDLLP
                     QIVADREKALSIGSEFIRLTRYIYGNDSDQENGIPRPDAVKPRSGEIFTLPAVGTISM
                     PEITLAKAIEQRRSLRKYSEAPLNLQELSFLLWSAAWARDFRTSERMEITLRNVPSAG
                     ARHPFECYLLVRNVEGIKPGLFYYHPVKHCLVRLETSEDIAGQIFDACLQQDMVKNAP
                     VTFIFSAVPYRTAWRYGQRGYRYLYLDAGHVGQNIHLAAEAIGAGACMIGAFQDEAMN
                     DAIAVDGVEEFVIYICSVGKK"
BASE COUNT          675 a          777 c          973 g          753 t
ORIGIN      
        1 attcagaggc agcagagaga ggcaaagagg gggcttgata atttatcatt aacaattgat
       61 tgaaatacgg ggtattggag ccggaaaggt cctcgtttcc ggagcgtgga aaaaccagtg
      121 gcaaagcaat taatcctgtg aagactcgag gccatgccgc aggcgctgat cgcgccttgg
      181 caaacgagga cgtttgccac tccacagggc atcgcaccgc gcctaactcg tgacgcttac
      241 ccttcacctt acaagcatca ctttccgggt ctcgctatcc tggcccgcgc tcagcttcag
      301 cagatagatc ccgcttggaa gcttgttgcc ggatgaatcc agcgcgtccc aggtgcagct
      361 ttgattctgg ccgggttccg cttttatttg ggtcctgtaa accatctgac ctttgaggtt
      421 gtaaactttc agttcagggg gcgccgtacg ctttagattc cagctcagat gcacgctctg
      481 tttgaaggga ttgggccagg ctatcagact tgtcaacgca ggcgcaactg gatcgtccac
      541 cgcggtgatg gggtcggtca catagagctt ggtggcaggt ctggcggtaa gcgcttcact
      601 ggctaccgga ggatcgtagg gatcatagat ggccgtgtcg ctcttgacgt tgcgggtgcg
      661 gatgatggag gtgttatcat ggtaaaagtc gtcggtggag gaatagtatt ggctgtctaa
      721 tggtcttagc accaggattg ccagattgga cccggcggtg tagataaaag gcgaggggaa
      781 gcaaaacatg ccgggactcg tccctggggt aaaatcggtc tctccgtcaa agacgagctg
      841 catttctgaa gcgggtatcc atcctgccga gaggttttcc agggtagtaa tccccatcca
      901 gatctggatc ggtttgatgc ctatgtcggg cgtattaatc tcagaaagat acttgacacc
      961 gtggatgagc ttatcctgtt ggggcagttc cgaggccaga tagagggtct cgcacatcga
     1021 ggtcttccaa tagaggtcga tgggatactt ccctgtatat tgcatatagt tctggatgcc
     1081 aaagctttca gcgccctcag ccagcacctt gacgattctc gaggggctct cgtcgttgga
     1141 agggtcaaga tctccggcgc agaccacttt ggcgctcagc ttgtggtttc cgggagtagt
     1201 gatattgtaa tcaaaacctg tacctcccaa aaacccggcc cagagagcgc cctggttgcc
     1261 ttgcgcgagg atgttcccac tgccgtcaag cagttgacag ctatagctat acacgtcttg
     1321 aatgccctga ttcaccaccc gggcggtgta atcctggttc tggcccacgt aatatgggtc
     1381 ccacacctcc agatgggtca cttcgaggtc ggtgttgtag ttgtagctca gttccacatc
     1441 gtcgatgagg atgccgccat agttgggatc ggcggatttg aaggccactc tgcgtcccat
     1501 ggtactggga tagcaggcca ggctgaggcg gtagcgtttc cagtagctgg tgagagggat
     1561 atcggtggtg aggctgaaaa agctggaggg atcggcaggg tcttgcagga tgcccacctg
     1621 gagctccgat tcatcctgat agctcctggc ccaccaccac acgctgaggt gccgaactgg
     1681 aaccgtttca tcaacaggcg ggagtgccag cagcacatag tcgtcgctgg gaccttcaaa
     1741 gaggatgatg tggttggggg cgctgtggct gttttcagcc tgggtctgag caagggagtt
     1801 ggattcacct gtctccaccg cgatccagcc atccggaagg ttgggtggca ggtataggtc
     1861 aaagttttgg gtgtagggca ccgggatggc cgccagggag gccagacctg tcaatagtgt
     1921 cataagtgcc aataccttga ggcccgaact gagcgctcgc ttcatagtga tctcctttat
     1981 cataacgatt gttgcactta taatacaaac agcttgcatc ccagccaggt tttcataacc
     2041 cggccgtctc tcaggcccct cccatgccta agcacgggat aagcgtgcga taagcgtgcg
     2101 ataagcacgg gatactgaag ggaagggata ctggttcagg gggatgcaaa gctcgcgggg
     2161 ataagcatca ttgtagccga atatgaagca ataattgctc cagattaagg ggaaatatct
     2221 atttcttgcc cacgctgcag atatagatca caaactcctc cactccgtcc accgcgatgg
     2281 cgtcgttcat ggcttcgtcc tgaaaagcgc cgatcatgca ggccccggcc ccgatggctt
     2341 cggctgcgag gtggatattc tggcccacgt gtccggcatc gagatagagg tagcgataac
     2401 cgcgctggcc gtaacgccag gcggtgcgat aggggacggc gctgaagatg aaggtgacgg
     2461 gggcgttttt gaccatatcc tgctgcaggc aagcgtcaaa tatctggccc gcgatgtctt
     2521 cagaggtttc gagtctcacc aggcagtgtt tgacggggtg gtagtaaaag agcccgggtt
     2581 tgatgccttc cacgttgcgg accaggagat agcactcgaa gggatgtctg gcgcccgcgg
     2641 aagggacgtt gcggagggtg atttccatgc gttcgctggt gcgaaagtcg cgggcccagg
     2701 cagcgctcca gagcaggaag gagagttcct gcaggttgag cggtgcttcg gaatactttc
     2761 tgaggctgcg gcgctgttcg atggctttgg cgagagtgat ttccggcatg gagatcgtgc
     2821 ccacggcggg cagggtgaag atttcaccgc ttctgggttt gacggcatcg ggcctgggaa
     2881 tgccgttttc ctgatcgctg tcgttgccat agatatagcg ggtgaggcgg ataaactcac
     2941 tgccgatgct cagggctttt tcacgatcgg ccacgatctg gggcagcaga tcgctcaggg
     3001 tgctttccag aaagtcatac tcctcttcac tgaggcgggc tttttccagg atgtcttctc
     3061 tggaatagtc tctggctttg tagtataggt attcaaaggt tttcatgtcc atggggcctc
     3121 cagggggggg ttatatttga taatataagc ggagtgagat ggtttcgcca tgtgagag
//
LOCUS       DAOSFN010000198         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_28900_length_3156_cov_9.389552, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000198 DAOSFN010000000
VERSION     DAOSFN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28900_length_3156_cov_9.389552"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1265)
                     /gene="dxr"
                     /locus_tag="PLO35_09415"
     CDS             complement(117..1265)
                     /gene="dxr"
                     /locus_tag="PLO35_09415"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOH79928.1"
                     /translation="MVERRRLALLGATGSIGSSTLEVLREQADRLQVVLASGGKNYQR
                     LLTLAAEFDIPLLFLTGIQDHAGQVRVRSLAGGRKVFFEEADLLNALREEDYDICLNA
                     IGASAGLSASYAVLTRGKPLALANKESLVMAGHILRPLWQKHGTPVIPVDSEHSAIFQ
                     AIGSHPSNELRRIHITASGGAFRTLPLEQFKDITPQQALKHPNWDMGAKVTLDSATMV
                     NKALEVMEAHWLFGLPYEAIDAVIHPSSIVHSLVEFVDGSILAQLSSPDMKLPILYAL
                     SWPQRWMSSLVMTDLLKVGPLEFHEIEAQRYPLYYLGLEAARAGGTMPTVFNAANEGA
                     LQLFLDGKIRFTQIFDRVEEALNRHQNIQDPDLETVLEVNRLVRIATG"
     gene            complement(1259..2212)
                     /locus_tag="PLO35_09420"
     CDS             complement(1259..2212)
                     /locus_tag="PLO35_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79929.1"
                     /translation="MKKPYILLLAWLALLPLMATSYAGEIFRLAPGVQNQAMGSTGLS
                     YHGSLASGWWNPALLATGSDSGAELMRAEHFEGLLQQNQLSLSLGGKTRTAINITHLA
                     IDKVKLTRLENSADTLSNSNRPIVWKTVTNQDFIITASFARSLSGSTSLGISPKLAYR
                     DLAGNSGYGFGADLGFLWNSGKGFLAAANLRDFFSTQVLWENGTHEVAIPSLDLETAY
                     QMKLFRSDMPLRIALRGEFYAEDRGEASNLQMGPVTADLHVGVMLQPIASLIVMGGYD
                     YDAITAGLGIRWNALGLDYAFKGSAPDGLGSTQRVSASYRW"
     gene            complement(2319..2394)
                     /locus_tag="PLO35_09425"
     tRNA            complement(2319..2394)
                     /locus_tag="PLO35_09425"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2359..2361),aa:Ile,seq:cat)
BASE COUNT          719 a          880 c          843 g          714 t
ORIGIN      
        1 cctaacacct aacacctaac cctaacaccc cctctccgta tctctctctc tgttacctct
       61 gtagctctgt agtgaaaaaa gaatctctgt aactcattag tatagcccgc cgttgctcac
      121 ccagttgcga tccgcaccag cctgttcact tccaataccg tctccagatc ggggtcctga
      181 atgttctgat gccggttcag ggcctcttct accctgtcaa agatctgggt aaagcgaatc
      241 ttcccatcca gaaagagctg cagagctccc tcattggcag cgttgaacac ggtgggcatg
      301 gtccctcccg ctctggcagc ctcaagtccc agataataaa gcggatatcg ctgagcttcg
      361 atctcatgaa actccagcgg gcccaccttt agtagatcag tcatcaccag gctggacatc
      421 cagcgctgag gccaggacag ggcatagagg atgggcagtt tcatatcagg ggaggagagc
      481 tgggccagga tgctgccatc gacaaattcc accagggaat gtacgatgga actgggatgg
      541 ataaccgcgt cgatggcctc atagggcagg ccaaagagcc agtgagcttc catcacctcg
      601 agggccttgt ttaccatcgt ggcgctgtcc aaagtcacct tggcgcccat gtcccagttg
      661 ggatgcttca gggcctgctg aggagtgata tctttgaact gctccagcgg cagagtcctg
      721 aaagctcctc ccgaggcggt gatgtggatg cgtctgagtt cgttggaagg atgagagccg
      781 atggcctgaa agatagcgct gtgttcacta tcgaccggga tcacgggagt accgtgcttc
      841 tgccagagcg gtctgaggat atggcctgcc atcaccagcg attctttgtt ggccagggcc
      901 agaggttttc ccctggtcaa aactgcatag ctggcactca gtcccgccga agctccaatc
      961 gcgttcagac agatgtcata gtcttcttct ctcagggcat tcagcagatc ggcttcctca
     1021 aaaaagactt ttcttccccc ggccagagag cggaccctca cttgcccggc atgatcctga
     1081 atccccgtca gaaacagcag cgggatatca aactccgcgg ccagagtgag cagcctctga
     1141 tagtttttgc cacccgaggc caggacgacc tgcagcctgt cagcctgctc cctgagcacc
     1201 tcaagggtgc tggaaccgat ggatcccgtg gcccccagga gagctaatct gcggcgctct
     1261 accatctgta gctggctgag accctctgag tggagcccaa tccatccgga gcgctgccct
     1321 taaaagcgta atccaggccc agagcgttcc agcgtatccc cagaccggct gtgatggcat
     1381 catagtcata accacccatc acgatcaggg atgcaatcgg ctgcagcatc acacccacgt
     1441 gcaggtcagc cgtcaccgga cccatctgga gattggatgc ttcgcctctg tcttcagcgt
     1501 aaaattctcc ccgcagcgcg atcctcagcg gcatatcgct cctgaagagc ttcatctgat
     1561 aagccgtctc caggtccaga ctggggatgg ccacctcgtg ggtgccgttt tcccagagca
     1621 cctgagtgct aaagaaatcc cgcagattgg ctgccgccaa aaaacctttc ccgctgttcc
     1681 acagaaatcc caaatccgcc ccgaaaccat aaccggaatt tcccgccaga tcacgataag
     1741 ccagcttggg agagatgccc agcgaagtgc tgccgctcag agacctggca aaggaagccg
     1801 tgatgataaa gtcctgattg gtcactgttt tccacacgat gggccggttc gaattggaca
     1861 gcgtgtcggc ggagttttcc aggcgggtga gcttgacctt gtcgatggcc agatgagtga
     1921 tattgatggc agttctggtc ttgccgccca ggctcaggga gagctgattc tgctgcagca
     1981 ggccttcaaa gtgctccgcg cgcatcaatt ccgcgccgct gtcagatccc gtggcaagaa
     2041 gggcgggatt ccaccatccg gaggccagag atccgtggta gctgagcccc gtggaaccca
     2101 tggcttgatt ttgcacacct ggggccaggc ggaagatctc tcccgcgtag gatgtggcca
     2161 tcagcggcaa caaagccagc caggccagca atagtatata tggctttttc atcgataatt
     2221 tcctataggg acaatatatc ccagctttca ctggtgtaaa gaaaaaactc cgcgtcaatc
     2281 acggaaaagg tgggatgaaa ccgacaaaga aagaaaaatg gtgggcccag agggagtcga
     2341 acccccgacc atccggttat gagccggaag ctctaccagc tgagctatag gcccttatgc
     2401 aaacataggt tttgtcctta aaattcaggg ggctgtttct gtcaagcgaa aagggggggt
     2461 aaagctggat agttttaggg ttatgaggct gtagagcaga atgtctttgc ggctggacat
     2521 cttaatgaaa ccgctgaata atctggatca ctggccatag agcttgtgcg aacaagccgt
     2581 cttggagtgc aaagcccctg gatataatag gcacttatct ccttgcgtga aaaggctttt
     2641 gatttaagtg ctgcgcatcg aagaccttta tctcgcgggt gtactggttg aagtccaggc
     2701 ggcccggaaa ctcgctgcca tatattctct tatcgtggcc gcttgaactc caccgggact
     2761 gggatgccac gccacaggcg ctgttcgcgc cttagcaaac gaggaccttt gctgttcctc
     2821 aagtatggat gtgagcctgg gtggatgcac tttggaatca agaacagtat ccccaccagc
     2881 cagaggaatg gcaaacgtct tgtttgccgt gacccggagg ggcactcaac atggcctcaa
     2941 agatgactaa tcaagtagtt tttttctggt ctgggaggcc atgcccaagt gctgctcgcg
     3001 cttttgctac cctgttggct gccctgtaga ttctgtactc cgttgccctt ttcttcctgc
     3061 atcctattac ggactcatta cggacttagt ccgtaatgag tccgtaatga ctccgtaatg
     3121 ctaagcggca agagtgcctg gatggtgtgc tgcata
//
LOCUS       DAOSFN010000199         3091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_29791_length_3091_cov_9.668972, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000199 DAOSFN010000000
VERSION     DAOSFN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29791_length_3091_cov_9.668972"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..2244)
                     /locus_tag="PLO35_09430"
     CDS             complement(7..2244)
                     /locus_tag="PLO35_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOH79930.1"
                     /translation="MNNAFRNDSLAKVTGRALYTDDLTMPGMLHAVPVYSPEPRAILK
                     GIDSSAVLKMPGVKAVFTYKDIPGSVRFGQIIKDYPTLADERINSTGDVLALIVAETR
                     DQAMAAIPLLKIDLEPLPAILDPEEAMKPEAAILHPDHGSNIKNYHCVRRGDLLAGEQ
                     DADLVIEQEFHTQRIEHSYMEPENSLCHLRPDGVMEVYGSMQHPFSTRRFVASTLGLE
                     LKDVEVKTIPMGGGFGGKDDTAALTCARAALCAWHLKTPVKMTLSREQSMRESYKRHP
                     YKMQYRMGLKQDGKITFVKVRMVADGGAYCSVTPWVTWRSTVQCCGCYEVPNVECDVY
                     GVYTNNVFCGAMRGFGSPQVNFAIEQLVEIAAGEMGMDEIEFRRLNMVRQGSTTVTGQ
                     VLDTHVVSLQEVMDKVLDKIDYHNKRARCSFGDPAQDKWYGIGLAISYRGMSLGAEGV
                     DLNSAVISVQPDGSILLETGIHENGQGSESAMILIASEELGIPVERIRYRLPSTANIP
                     DGGTTVASRGTIMGGGATVNAARQLKRIVTEAVKTHLNREVSSFRDQVLLDASGELIC
                     SWNDAIRLCYSNQIYPYAFGVFQAPKVDWDEHTGQGNAYFTWVYGCQAVELEVDSKTG
                     AVKLLNMVAAHDVGKAVNPALVEGQFYGGMAMATGYALFEDCAVENGLVKPTNFHNYR
                     LVRATDMPEMTAIIVENPDPNSPSKAKGIGEPTNELMAPAIANAVYHATGKRFTSLPI
                     RVVKQ"
     gene            complement(2282..>3091)
                     /locus_tag="PLO35_09435"
     CDS             complement(2282..>3091)
                     /locus_tag="PLO35_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79931.1"
                     /translation="LLLSWGCAHIDPTVLESAEALPAGTLRVSAGGGLSQNYITGLDI
                     PEDALWDNEDALLPDGNIGFIARPKLKVGLGKNWEAALSGGFSFAPDSTMGFMDTDTN
                     TAWQLKASAKKVWDLNPCHSLAAIPTLAYSDGAWESKQAADGHQLWMGAQTLSFELPL
                     VSSWHGRYKGYKYNLNFTLRPAYTALWRSRNYADWSFSLPAGGEYWSTQEELEREDVF
                     RVGFLAGLEDKGEKGNTIFELGLDLISHSDKVYLMPLIGVSHQFGDYGKRD"
BASE COUNT          612 a          866 c          909 g          704 t
ORIGIN      
        1 tatctctcat tgttttacca ctcgtatcgg taagctcgta aaccgcttcc ccgtagcatg
       61 atagacggcg ttggcgatcg cgggggccat gagttcgttg gtgggttcgc cgatgccttt
      121 ggctttggag ggggaattgg gatcggggtt ttccacaatg atggctgtca tttccggcat
      181 gtcggtggcg cgtaccagcc tgtagttgtg gaagttagtg ggtttgacga ggccattttc
      241 caccgcgcag tcttcaaaga gcgcgtaacc tgtggccatc gccatgccgc catagaactg
      301 gccttccacc agggcgggat tgacggcttt gcccacgtcg tgagcggcca ccatgttcag
      361 cagtttcacg gcacccgttt tggagtccac ttccagctcc acggcctggc agccgtaaac
      421 ccaggtgaaa taagcgtttc cctggccggt atgctcgtcc cagtccacct tgggggcctg
      481 aaaaacaccg aaggcgtagg ggtagatctg attggagtag caaagccgga tggcatcgtt
      541 ccaagagcat atcagttcac cggatgcatc cagcaggact tgatcgcgga agctgctgac
      601 ctccctgttc agatgcgtct taactgcttc agtgacaatt cttttgagct gacgggccgc
      661 gttgaccgta gctccgccgc ccatgatagt accgcgtgag gccaccgtgg tgccgccgtc
      721 cggaatgttg gcagtggagg gcaggcgata gcggatccgt tccacgggaa tgccaagctc
      781 ttccgaagcg atcaggatca tggcggattc tgagccctgg ccgttttcgt ggatgccggt
      841 ttccagcagg atggagccgt ccggctgcac actgatcacg gcgctgttga ggtctactcc
      901 ttcggccccg aggctcatgc cgcgatagct gatcgccagg ccgatgccat accacttgtc
      961 ctgagcggga tcgccaaagc tgcatcgggc gcgcttgttg tgatagtcga ttttatccag
     1021 caccttgtcc ataacctctt gcaggctgac gacatgggtg tccagcacct ggccggtgac
     1081 ggtcgtggaa ccctggcgca ccatgttgag gcggcgaaac tcgatctcgt ccatgcccat
     1141 ttcgccagcg gcgatttcta ccagctgctc gatggcaaag ttgacctgag ggctgccaaa
     1201 gccacgcatg gcgccgcaaa agacgttgtt ggtatagacg ccatagacgt cgcactccac
     1261 gttgggcacc tcataacagc cacagcactg aacggtggaa cgccatgtca cccagggggt
     1321 tacactgcag taggctcctc cgtccgccac catgcggact ttcacaaaag tgatcttgcc
     1381 gtcctgcttt aagcccatgc ggtattgcat cttgtaggga tggcgtttgt agctttcgcg
     1441 catgctctgc tcacggctca gggtcatctt gaccggggtc ttgagatgcc aggcgcaaag
     1501 cgctgcccgg gcacaggtta aagccgcggt gtcgtctttg ccgccaaaac ctccgcccat
     1561 ggggatcgtt ttcacctcca cgtccttgag ttccagaccc agagtggagg ccacaaagcg
     1621 tcgggtcgag aagggatgtt gcatgctgcc atacacttcc atcactccat cgggcctcag
     1681 atggcagagc gaattctccg gttccatata gctgtgttcg atgcgctggg tgtgaaactc
     1741 ctgctcgatc accagatcgg catcctgttc tccggcaagc agatcacccc ggcgcacgca
     1801 gtgataattc ttgatgttgc tgccgtgatc gggatgcagg atagcagcct cgggtttcat
     1861 ggcctcttca gggtccagga tggcgggcag cggctccaaa tcaatcttta acagggggat
     1921 agcggccatg gcctggtctc tggtttcggc cacgatcaag gccaggacgt ctccggtgga
     1981 gttgatccgc tcgtccgcca gggtgggata gtctttgatg atctggccaa aacgaacgct
     2041 gccggggata tctttgtaag taaatacggc ctttacgccc ggcattttga ggaccgcgct
     2101 gctatcaatg cctttgagga tggctcgcgg ttcgggacta tagaccggca cggcatgcag
     2161 catgccaggc atggtcagat catcggtgta gagagcccgg ccggtgactt tggcgaggga
     2221 gtcgttgcga aatgcattgt tcataggggt gtcctgtgtg taaagtgatg atatgggggc
     2281 tttaatcccg cttgccatag tctccaaact gatggctgac accgatgagg ggcatcagat
     2341 agactttatc ggagtggctg atgaggtcca ggcccagctc aaagatagtg ttaccttttt
     2401 cgcccttgtc ttccaaaccg gccagaaagc ctacacggaa gacgtcctcc cgctccagtt
     2461 cttcctgcgt ggaccaatac tcacctccag ccggtaaact gaagctccag tccgcgtagt
     2521 tgcggcttct ccacagagca gtgtaagcgg gacgcaaggt gaagttgaga ttgtacttat
     2581 atcctttata gcgaccgtgc cagctgctta cgagcggaag ctcgaaactg agcgtctgag
     2641 ctcccatcca gagttgatga ccatcggctg cctgcttgga ttcccaggcc ccgtccgagt
     2701 aagccaaagt gggaatggcg gccaggctgt gacagggatt gaggtcccac accttcttgg
     2761 ctgaggcttt gagctgccag gcggtattgg tgtcggtgtc cataaagccc atggtggaat
     2821 ccggcgcgaa ggaaaatccc ccggaaagcg ctgcctccca gttctttccc agccccactt
     2881 tgagcttggg acgagcgatg aaaccgatgt ttccatccgg caggagagcg tcctcgttat
     2941 cccacagggc atcctcagga atatctaacc cggtgatgta gttctggctg agaccgcctc
     3001 ccgctgagac tctaagtgta ccagccggaa gggcttcggc gctttccagc acggtgggat
     3061 cgatgtgggc acagccccag gacaatagca g
//
LOCUS       DAOSFN010000200         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_30016_length_3074_cov_9.799603, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000200 DAOSFN010000000
VERSION     DAOSFN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30016_length_3074_cov_9.799603"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..715)
                     /locus_tag="PLO35_09440"
     CDS             complement(299..715)
                     /locus_tag="PLO35_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79932.1"
                     /translation="MKKATKAILFSLGGTALGLIAAAIGKAMSHYKFYKQAGVMDLIK
                     EMYPDENWDKRNLFLHLKGFDLKLYATDEFLAANPDAEAKIMSALLEKAPSLEKLRCV
                     IKVLPVSELKTRCGKGVMFECRKDKEPELPETEEES"
     gene            complement(888..1424)
                     /gene="rsmD"
                     /locus_tag="PLO35_09445"
     CDS             complement(888..1424)
                     /gene="rsmD"
                     /locus_tag="PLO35_09445"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOH79933.1"
                     /translation="MRVITGRFKGANLLSVPGRSTRPTTSFHREVMFSMYQDFEGKRV
                     LDLYAGTGGLGLEALSRGAVWVDFVEFASVAIAVLLQNIQKLRCASECHIHRRKVEAF
                     LKVFDTPYDLIFMDPPYDKDLVNPTLRQIFERGVLNPGGVVIVEHSGREAIAPEFGPY
                     LLRSKPGKITSLSLLQRH"
     gene            complement(1435..1608)
                     /locus_tag="PLO35_09450"
     CDS             complement(1435..1608)
                     /locus_tag="PLO35_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79934.1"
                     /translation="MADKSDKKYSKVCQLCQRDCKQDEAITVVSCPRFIKAPVQLMVP
                     LKFHPGRPKKAKA"
     gene            complement(1608..2180)
                     /locus_tag="PLO35_09455"
     CDS             complement(1608..2180)
                     /locus_tag="PLO35_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79935.1"
                     /translation="MKTNVRAWTPTLTLARLFEEQNQFFDALAAYELISQTDSSPAVR
                     ERIEALHIRILNDPSNRYDPRIEKLFSPEELAYLKILDHNGFENMAAAAQKLGEGALS
                     EDIFIDEDEDFRLEADGDPDALDELLREIEEQSQLKMLDDSSTVKDHTLRDLMVALLS
                     KYERDTPLADIRLSDLISVLLDLQNTLPKS"
     gene            2340..2738
                     /locus_tag="PLO35_09460"
     CDS             2340..2738
                     /locus_tag="PLO35_09460"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HOH79936.1"
                     /translation="MSDDTRWVQRFSNYRKALTLLEAAALIQMPSHIETEGMIKRFEY
                     TFELAWKTLQDYLSEMGFKDFAGPRDTLKLAFANGVITDGQAWMDMIQARNLSSHTYD
                     IKTAKKLEADIRNLFLARFIELRSYLANKL"
     gene            2735..3031
                     /locus_tag="PLO35_09465"
     CDS             2735..3031
                     /locus_tag="PLO35_09465"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOH79937.1"
                     /translation="MKAYGIYQETWDQILSVLRSESKVREIILFGSRAKGVFKEGSDI
                     DICIKGKDLGYDDLIRLNGLLDELDLPWEIGLLIHDMIDEPMLIEHIARVGIKL"
BASE COUNT          715 a          778 c          846 g          735 t
ORIGIN      
        1 tcattacgga ctcattacgg gtgaagtccg taatgagtcc gtaataggat gcgggaggaa
       61 ccaggtaaga ggctgcgcat caatcgtatt gtcaatggtt ttgtcaatgg tcttattcag
      121 taaaaatccc ccaaaaaatc cagcaaaacg ctgctggaat gctagcctat gattatgcta
      181 aacaactgcc ctgatgggct caaaccaccc tcatcccgat ggctggagat tgttttctgg
      241 ccagatctat agccagcgct gcccaattcc tctcagaata aaaacccgct attgccgctc
      301 agctttcctc ttccgtttca ggaagctccg gctctttatc tttcctgcat tcaaacatga
      361 cgcccttgcc gcatctggtc ttcaactcgc tgactgggag tactttgatt acacagcgca
      421 gcttttccag actgggtgct ttctccagca gggcgctcat gattttggct tccgcgtccg
      481 gattggccgc gaggaattca tccgtcgcgt agagcttgag atcaaaaccc ttaagatgca
      541 gaaagagatt gcgtttatcc cagttttcgt ccggatacat ttctttgatc aggtccatga
      601 ccccggcttg tttgtagaat ttgtaatgag acatggcctt gccgatggcg gccgcgatca
      661 atccgagcgc ggtgcctccc aaagaaaaca ggatggcttt ggttgccttt ttcatgggtt
      721 cctcctggta ttattcctat ggaataagat aagctaaaat attggaattt ccaatggata
      781 ttctcaaccc cgccattttg aagcgacgat ccgcccagga atcctccccc tgcgcgtcct
      841 gtggtttatg ccgtaaagaa aaaccggagt agaatgagaa tacgatccta gtggcgctgc
      901 aacaggctca gagacgtgat cttgccaggt ttggagcgca gaagatatgg tccaaactca
      961 ggggctatgg cttcccggcc gctgtgctcc acgatgacca ctcctcccgg gtttaggaca
     1021 ccccgctcaa atatctgtct cagagtggga ttgacgaggt ccttgtcata gggcgggtcc
     1081 ataaaaatga ggtcataggg cgtgtcgaag accttgagaa aggcctccac cttgcggcga
     1141 tggatgtggc attcgctggc gcagcgcagc ttctggatgt tttggagcag gacggcgatg
     1201 gcaaccgagg caaattccac aaagtccacc cacacagccc ctctggaaag ggcttccagc
     1261 cccaatcctc ccgttccggc atagaggtcc agcacgcgtt tgccctcaaa atcctgatac
     1321 atgctgaaca tcacttcgcg gtgaaaagag gtggtgggac gggtactccg gcctggaaca
     1381 gacagcagat tggcgccctt gaagcgaccc gtgatgacgc gcatgctctg cccctcaggc
     1441 cttggctttt ttgggccggc cgggatgaaa cttgagcggc accatcagct ggaccggagc
     1501 tttgataaag cgcggacagc tcaccaccgt gatggcttcg tcctgtttgc agtcgcgctg
     1561 gcagagctgg cagaccttgc tgtatttttt gtcggattta tcggccatca ggatttgggg
     1621 agagtgtttt gcaggtccag cagcacggaa atcagatcgc tgagacgaat atcggccagg
     1681 ggggtgtcgc gttcgtactt gctgagcagg gccaccatga ggtcccgcag ggtgtggtct
     1741 ttcacggtgg agctatcgtc cagcattttg agctgggatt gttcttcgat ctcgcgcagc
     1801 agctcatcca gagcgtccgg atcgccgtcg gcttcgaggc gaaaatcctc atcttcatcg
     1861 atgaagatat cttcgctgag agcgccctcg cccagcttct gagccgcggc ggccatgttt
     1921 tcaaagccgt tgtggtccag gatcttgagg taggccagct cttcgggaga aaagagcttt
     1981 tcgatgcggg ggtcatagcg gttggaggga tcgttcagaa tacggatatg cagggcctcg
     2041 atcctttccc tgacggcagg tgaggaatcg gtctggctga tcagctcgta ggcggccagc
     2101 gcgtcgaaga actggttttg ctcttcgaag aggcgggcca gggtaagagt aggcgtccag
     2161 gcgcgtacgt tggtcttcat cgatctctcc cgagtgaatt tatcttaatt ttgcaccaat
     2221 gtataaaagg aggctttgtg tcaatagaaa agatgccctg ggagcctcca ggcagaaatc
     2281 acttgactgg atatggcggg cgggaaaaac tcggacattc tgattatctg ggagtgaata
     2341 tgtcagacga tacccgctgg gtccagcgat tcagcaacta ccgaaaagcg ttgaccctcc
     2401 tcgaggccgc tgctctgatc cagatgccct cccacatcga aaccgagggc atgataaaac
     2461 ggtttgaata cactttcgag ctggcgtgga agaccctgca ggactatctg agcgagatgg
     2521 ggttcaagga ctttgccggt ccccgggata cgctgaaact ggcctttgcc aacggtgtca
     2581 taaccgatgg ccaggcgtgg atggatatga tccaggcccg caatctcagt tcccacacct
     2641 atgatatcaa gacggccaaa aaactggagg cggacatacg taacttattc ctggccaggt
     2701 tcattgagct taggtcctat ctggcgaaca agctgtgaaa gcgtatggaa tttatcagga
     2761 gacctgggac caaatcctga gcgtgctcag aagtgaatct aaagtaaggg aaatcatcct
     2821 ctttggttct cgtgccaaag gggttttcaa agaaggctcg gacatagata tctgcatcaa
     2881 aggcaaagac ttgggctacg atgacctgat ccgcctgaac gggctactgg acgagcttga
     2941 tcttccctgg gagattgggc tactgatcca tgacatgatc gacgaaccaa tgttgattga
     3001 acacattgcc agagtgggaa ttaagctatg atcgcggaat ggggctggct cttgaaaaaa
     3061 cagacctaga ggcc
//
LOCUS       DAOSFN010000201         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_31029_length_3002_cov_9.769936, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000201 DAOSFN010000000
VERSION     DAOSFN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31029_length_3002_cov_9.769936"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..427)
                     /locus_tag="PLO35_09470"
     tRNA            complement(353..427)
                     /locus_tag="PLO35_09470"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(393..395),aa:Asn,seq:gtt)
     gene            763..>3002
                     /locus_tag="PLO35_09475"
     CDS             763..>3002
                     /locus_tag="PLO35_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH79938.1"
                     /translation="MKSASILVFLIICSSLLAVDLINETFPTTGVIPSGWTRTGTNAA
                     AWSVFGTNFAGGTASELRLYWTPGAMGQTRFISPPVDTRKVHDMTLSFRHCLDDFSTT
                     QTYTIGVQISHDLSNWTTLWSVTTSSDIAAEQVNLDIDYTLGMSQTTYIAFWFNGDNN
                     NIDGWYIDNVLLSYQDTLGSGTWAAGTYLIDGNLIVPPGYTFTIPGGSTLLFDTGKSL
                     SVQGSLMANGDYGYPVTFTSLGSGATWAGISMMNLGSTQDSTIVKYSMIERCTERALT
                     IYSTNRVRFKGCVIANCDSNTDYGIVLLYETNAIVEECTFLENENDIGPIFYCYLASA
                     IIRNNTFFNNNIGPSLIKLQSYNLSNYTGNSIYQNHTWSGYGVALHIKDCSGTLYRQM
                     IANNMTHGIWVQGTGNTAYITNCLLVNNYGWGLINEGRGAVRSSILWGNNSGAIQNTH
                     TGLTIRYSCVAGGYYGINGTLPLGENYTSSTSSNPLFVSPSPTSDDTSNPTFYNWTLQ
                     TTSPCIDTGDPAHPVDEDGSRVDMGMYSRKLKPIINRAADVPNDQGHQLDLKWLRNDL
                     DVSYQPGAFYSVWREDGVRNGAVLLGSLNELPAALNSKQQDICWWDGDRTWYYLSQVP
                     AFNFGDYGLVVSTLQDSSSTGTHASNYKVIYSNAQGYWQSIPQSGYTVDNIPPLPAQR
                     LTLQASAPNQYLLAWDEVTEGIWEGNSYPEQNQVFYKVYASTDPSVPILPENLIGITA
                     SPQMLI"
BASE COUNT          745 a          816 c          762 g          679 t
ORIGIN      
        1 tttcgcgata cttcacgggg ggctataaca tttagacgcc cttccagggc ttcaatttgg
       61 ccattttcgc acaagctcgg agacgggagt ccagcttgca tacgtcgctg atctgcctgg
      121 cgcagagaga tttgtgctac aagtattatt atgcgggcat cagatgaagg cggaaactga
      181 tgtatttcca ataatcccga gacaactgtg gcatagtcca cctctgcgac ggagagaaag
      241 tgacggcaga caagggcacg ggagcggttt tccgcttcca gcctggcatt gccagccctg
      301 gaatcccctt gatctgagat cagacagcca aaaaaaaccc ggataaaaaa aatggctccg
      361 gaagagggat tcgaaccccc gacctagtgg ttaacagcca cccgctctac cgctgagcta
      421 ttccggaacg cattcgtgag gagcaaagtc tctgggggcg gtaaaattgt caatagaatt
      481 gtttggacgg atatgcccct gacagggtag acgcccagga tgagcgggcc ttccgggctt
      541 gatgaatact cccaagtgat caggtgttcc aagagtgact atggattgac ttacaatggg
      601 ctgtgagcat aaaaaaaccc gtgaacgccg atagatcagg ctactggaaa tctctcatgg
      661 ccatcaggat acatcatctt caccggcgtg attttatcct tgacagtttt tgccatgtat
      721 ccggaatgaa ggcataatag attcatgctg aaggaggata tcatgaaatc agcatctatc
      781 cttgtttttt tgattatatg ttcgtcattg ctggcggtgg atctcatcaa cgagaccttt
      841 cccactacag gtgttatccc aagcggctgg accaggactg gcaccaatgc cgcggcctgg
      901 agtgtgtttg gcacaaactt cgcgggaggt acagccagcg aactgcgctt gtattggact
      961 cctggtgcga tgggccaaac ccgctttatt tctcctcctg tcgatacccg caaagtgcat
     1021 gatatgaccc tgagtttcag acactgcctg gacgacttta gtaccaccca aacctacacc
     1081 atcggggtgc agatatccca tgacctgagc aactggacca ctctgtggtc cgtcaccact
     1141 tcctccgata tcgcggcgga acaggtaaat ctggatattg actacacgct gggcatgtcc
     1201 caaaccacct acatcgcctt ctggttcaat ggggacaaca acaatatcga cggatggtat
     1261 atagacaatg tgctgctttc ctatcaggac actctgggca gcggcacctg ggcggccggg
     1321 acctacctga tagacggcaa tttgatcgtc ccgcccgggt atacattcac cattccagga
     1381 ggatcaaccc tgctttttga tacgggcaaa tccttatcgg tgcagggcag cctgatggcc
     1441 aatggcgatt atggctatcc cgttactttc acgtctctgg gcagcggggc cacctgggct
     1501 gggatatcca tgatgaacct ggggtccacg caggattcca ccatagtcaa atactccatg
     1561 atcgagcgtt gcaccgaaag ggctttgact atttattcca ccaatagagt gcgcttcaaa
     1621 ggctgtgtga tcgccaactg cgattcgaat accgattatg gcattgtgct cttgtatgaa
     1681 accaatgcca tcgtggaaga gtgtactttc ctggaaaacg agaatgatat cggccccata
     1741 ttctattgct atctggccag tgccatcatc aggaacaata cattcttcaa caataatatc
     1801 gggccttcat tgatcaagct ccagagttac aacctgagca attacactgg caacagcata
     1861 taccagaatc acacctggtc tggatatggc gtggcgttgc acatcaagga ctgcagcggg
     1921 accctgtacc ggcaaatgat cgctaacaat atgacgcatg gaatctgggt acagggtact
     1981 ggcaatacgg catatatcac caattgtctg ctggtgaaca attacggatg gggcttgatc
     2041 aacgaaggcc ggggagcggt ccgcagcagc atcctttggg gtaacaacag tggtgccatc
     2101 caaaacaccc ataccggcct cactatccgc tattcctgtg tagctggcgg ttattacggc
     2161 atcaacggga cgcttccttt aggagaaaac tacaccagca gcaccagttc caaccccctg
     2221 tttgtgtccc cttctcccac ctcagacgat acttccaacc ccacgttcta caactggacc
     2281 ctgcaaacca cctcaccctg tatagacaca ggcgatccag cccatcctgt ggatgaagac
     2341 ggcagcagag tggatatggg aatgtattcc cgcaagctga agccgataat caacagggcc
     2401 gcggatgtgc caaacgatca gggccaccag ctcgatctga aatggctgcg caacgacctg
     2461 gacgtcagct accagcctgg agcgttctac tccgtctgga gagaggacgg agtgaggaat
     2521 ggcgctgtgc tgctgggtag cctgaatgaa ctgcccgccg cgctgaacag caagcagcag
     2581 gatatctgtt ggtgggatgg ggaccgcacc tggtactatc tttcccaggt gccggccttc
     2641 aactttgggg attatggcct ggtggtttcc accctgcagg attcctcctc cacgggaacc
     2701 cacgcgtcca actacaaagt gatctattcc aatgcccagg gttattggca atccatcccc
     2761 cagagcggct acacggtgga taacatccct ccccttccag cgcagcggct cactctacag
     2821 gccagcgctc ccaaccaata tctgcttgcc tgggatgaag ttacggaagg gatctgggaa
     2881 gggaacagct atccggaaca gaatcaggtc ttctacaagg tctatgcctc cacagaccca
     2941 agcgtgccca tcctgccgga gaacctgatc gggatcacgg cctcaccgca gatgctgatc
     3001 aa
//
LOCUS       DAOSFN010000202         2910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_32490_length_2910_cov_9.131699, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000202 DAOSFN010000000
VERSION     DAOSFN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32490_length_2910_cov_9.131699"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLO35_09480"
     CDS             complement(<1..829)
                     /locus_tag="PLO35_09480"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH79939.1"
                     /translation="MKTSALALLIICLCGSLFGGISIKMPTGESVQLYSEYVAIVIGN
                     SDYQYYRKLPGAIKDVEAIAETFEGLGIRVIKKFNLKSSDMITLFNEMVDEYGKDRER
                     ALLFYFAGHGETERMAGGRELGYILPVDTPMLQGNPSGFRSKAISMTRVNEFAELIQS
                     KHVLMFFDSCFSGTVFTTRRAAPQIISQKTKEDVRQFITAGAAEETVPDQSIFRATLV
                     DGLSKGYADTNKDNYITGEELGFYLENEVVNYSGGGQHPKYGKIANIALDKGDFVFAL
                     "
     gene            complement(1314..2033)
                     /locus_tag="PLO35_09485"
     CDS             complement(1314..2033)
                     /locus_tag="PLO35_09485"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOH79940.1"
                     /translation="MKHLDTQSRVLVLTGAGISAESGIRTFRDAGGLWEDHRVEDVAS
                     PEGFQNDPQLVWSFYRQRFRQALETQPNPGHLALKRMEEALGDRFWLVTQNVDGLHED
                     AGNKNLIEMHGSLHRCFCTGCGKIHQTAEIDLDNPLPYCSACEMLLRPDIVWFGEIPY
                     QLGRIEQLLKKSDLFLIVGTSGTVYPAAGFVMTARYFGAKTVAVNLDPPHNSSFIDEF
                     HQGKAGDILPGLVDKWLRILT"
     gene            complement(2159..>2910)
                     /locus_tag="PLO35_09490"
     CDS             complement(2159..>2910)
                     /locus_tag="PLO35_09490"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79941.1"
                     /translation="TPGGSYSMKFGGSGSGQYGNSALGALDSPEFMLGMNSMLKFSHW
                     MDAEAHSTPTYAWDGGLVQMSIDGGEWIQISPVGGYTHRIYQNNASPFAANTNCWSGS
                     FAWTEATFDLSSYSGNARFRFLFGTDGAVTGEGWYVDDIRVESEFVDNSDPAVSPLRF
                     DLNANYPNPFNPSTTISFSVPASGEVELAIFNLKGQRVKTLVSGELAKGQHSVVWNGL
                     DNAGRSVASGVYLYKLRSGTELKTRKMMLMK"
BASE COUNT          698 a          760 c          744 g          708 t
ORIGIN      
        1 tgagtgcgaa cacaaaatcg cctttgtcca aagcaatgtt ggcaatcttg ccatatttgg
       61 gatgctggcc acccccggaa tagttgacca cctcgttttc cagatagaat cctaattcct
      121 caccggtgat atagttgtct ttgttggtat cggcgtagcc tttgctgagc ccgtccacca
      181 aagtggcgcg aaaaatactc tggtccggaa cggtttcttc cgcagcgccg gcagtgatga
      241 actggcgcac atcttccttg gttttctgag agataatctg aggagcggcc ctgcgagtgg
      301 tgaacaccgt gccggaaaaa cagctgtcaa aaaacatcag cacatgcttg gactggatca
      361 attctgcgaa ttcgtttacc cgggtcatgg atatggcttt agacctgaag ccgctgggat
      421 tcccttgcag catgggagtg tcgacaggca ggatatatcc cagttctctg cctcccgcca
      481 tcctttccgt ctctccatga ccggcaaaat aaaagagcag agcgcgctcg cggtctttgc
      541 catattcatc gaccatctcg ttgaagaggg tgatcatatc agagctcttg aggttgaatt
      601 tcttgattac cctgatccct aagccctcaa atgtctcggc gatggcttcc acgtccttga
      661 tcgctcccgg gagcttgcga tagtattggt aatcgctgtt acctataaca atagccacat
      721 attcagagta caattgaacg ctctctccag tgggcatctt gatcgaaatg ccgccaaaca
      781 gagagccgca caagcaaata attagtaatg ccagcgctga tgtcttcacc tttcctccca
      841 tcagtatgtg ctcttaagct tttttaaaac agccgtttaa cctgatcgac ggcgccaatt
      901 accagtcaag cgactgcctt ccaatcacgc tataaatcta atgcgcgcac tgtgaactgc
      961 ctgcgcgatt ctgtcaacat atttctttgc ggaattgagg cgataaactt ggagcagaaa
     1021 agattatata attatcactg gctgaatggc atgcttatca tgcaaaaact gaagcggatg
     1081 tgatgaatga tcttccacag gaataaaatg aatggtttgt ggaagcagcg cgcgcaattg
     1141 gatatagctt cccaagcagt atctctcctg ttcgctccag gctcccccag ggcatcccca
     1201 aggtgcttgg ggatgccttg gaccagaccg ggcggggcaa gagtgccatg agtaagggcg
     1261 cttccagagt ggaaacaaaa aaagcccctc ggttgagggg cttctacaga tgcttatgtc
     1321 aatatacgca gccatttatc cacgaggcca ggcaggatgt cacctgcctt gccctgatgg
     1381 aattcatcga taaaggagct gttatgaggc ggatcgagat tgaccgccac ggttttggca
     1441 ccgaaatatc tggcagtcat cacgaatccg gcggcgggat agaccgttcc actggttccc
     1501 acgatcagaa agagatcgct ctttttaagg agctgctcta tccttccaag ctggtaggga
     1561 atttcaccaa accacacaat atcaggcctc agcagcattt cacaagcgct gcagtagggg
     1621 agagggttat cgagatctat ttcggcggtt tgatggatct tgccgcagcc agtgcagaaa
     1681 cagcggtgca ggctgccgtg catctcgata aggtttttgt tgccagcgtc ttcatgcagg
     1741 ccgtccacgt tctgcgtgac cagccagaag cggtccccaa gggcctcctc catccttttc
     1801 aaagccaggt ggcctggatt gggctgagtt tcaagcgctt gacgaaagcg ttgccggtaa
     1861 aaactccaca ccagttgagg gtcgttttga aacccttccg gtgaggcgac atcctcaacc
     1921 cgatggtctt cccaaaggcc tccagcatcg cgaaaggtgc ggattccgct ttccgcgctg
     1981 attccggctc cggtgagcac caggacccgg gattgagtat caagatgctt catggtttaa
     2041 agactgcggc tcaggagttt cggattctca gcagaaagaa tcaggagggt cggatcgcaa
     2101 tctgaccctc cggtatactt ccacgtaaat gtatacgctt gctgctccac aaatgggatc
     2161 acttcatcaa catcatctta cgggttttga gctcggttcc gcttctcagc ttgtagagat
     2221 acaccccgct ggcgacactg cgtcccgcgt tgtccaaacc gttccataca acactatgct
     2281 gccctttggc aagctcgccg ctgaccaggg tcttcactct ctggcctttg aggttgaaga
     2341 tcgcgagttc cacctcgcca gaggcgggaa cgctgaaact gatggtggtg ctggggttga
     2401 aggggttggg atagttggcg ttcaaatcga aacggagagg tgagactgcg gggtctgagt
     2461 tgtccacaaa ttcagattcc actctgatgt cgtccacata ccagccttcg cctgtaacgg
     2521 ccccatctgt tccaaacagg aagcggaatc gagcgttgcc ggaatagctg gagagatcga
     2581 aagtggcttc tgtccaggcg aaagaaccag accagcaatt ggtgtttgcg gcaaaaggcg
     2641 aggcattgtt ttgataaatg cgatgagtgt atccgcccac tggagagatt tgaatccatt
     2701 ctcctccatc gatactcatc tgcaccagtc caccgtccca agcataagtt ggagtgctgt
     2761 gagcctcggc atccatccag tgggagaatt tcagcatgct gttcattccc agcataaatt
     2821 ccggagaatc aagagctccc aaagcgctgt tgccatactg accggaaccg gaaccaccga
     2881 atttcatgga gtaactgcca ccgggggtgt
//
LOCUS       DAOSFN010000203         2774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_34829_length_2774_cov_8.203016, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000203 DAOSFN010000000
VERSION     DAOSFN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34829_length_2774_cov_8.203016"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..2553)
                     /locus_tag="PLO35_09495"
     CDS             complement(295..2553)
                     /locus_tag="PLO35_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH79942.1"
                     /translation="MQKSLIITILCLLISYLSATPRGTTQAEIPCGTTVYDKLTDLGL
                     NQFRLRHPNYAPAIPGRMDREQWVSCDSLSLNSILPAGEHMGIQYFAAGDDFSYYRSF
                     GNLLPAARNAVAKAPVWIKPRLEYTLSCLNQDRQLLFAGLINSASDPYVDEIAFGVAV
                     SSPQYLNSSIAWPQLFTLNAQRIYSAAADLPYVEVIDTGSSTTGADYWSTTSYTKKDA
                     DGTIRSVAVPKDIYYWYIVLPKLTDEIPAFVDPSIVENNSSHTNNATGPDTGKFWRAM
                     LYNEPDDGYPAMADTLVQIQTLRDLSGRGPDAIRALQWWINASMSFTSNSERPHQPAR
                     IYRKHFGRCGEYADLTAAAARTALIPCTSILSMSTDHTWNEFWDEEWTMWEPVNGYID
                     VPMVYEQGWGKVFGSVFEIRGDGYLASVTGTYSSGLAQINLMVKDQEDRPVDGARVIL
                     AIQEGGMKTDMVGFTDNQGLVSFPVGENRSYYARAETSFGIYPPIAGTYSELVQNVAD
                     GAVHYFEMEIPGSLPVITINPVEPPSVPDPLRRFQTQYQVPGYYISGRVTWDDIFSLG
                     DYPDFLQPVAQPGELNLLVTDADNYIFWDVMQTCDAYLAQTASAGSTATFNLPAVGDH
                     YAILDNSHRHGNAQQVNGFLLGQLWGTTPNADPQQTPSPIRLSVYPNPFEDSVLFQYT
                     LPKASPVGLEVYNLRGQKLITLIPEQDMTSSGVISWDGRDSQGRLCAAGIYLLRLNSG
                     GESSTIKLLRLK"
     gene            complement(2593..>2774)
                     /locus_tag="PLO35_09500"
     CDS             complement(2593..>2774)
                     /locus_tag="PLO35_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOH79943.1"
                     /translation="TRTIQKFINPTGPETGTQRVVRGGSVMNGEGRNLNILYREKGNP
                     NRGVQYVGFRLVRTK"
BASE COUNT          615 a          707 c          819 g          633 t
ORIGIN      
        1 tcccgccggg actcgtaagg aataatgcca cgataatcag tttttgcaca agctctgtag
       61 ccagtgttac ggctatgtgc aacggtcttt ccatatatat ctttactctg gccggttgaa
      121 ctccaacggg gtcgaaatat caccactggc gggactcaat cgcgaatcta tggataggat
      181 gattccatag ctctatcgcc tcttgaccct ttcgcctcag cttagccaga ccaaatcggg
      241 accaaagccc ataaatgggc tttggtcccg ataagatctg cctgaaagcg ggctttactt
      301 taaacggagc agtttgatgg tacttgactc gccaccggag ttgaggcgca gtagatatat
      361 cccggcggcg caaagccttc cctgagaatc tcttccatcc cagctgatta ctccggaact
      421 ggtcatgtcc tgctcaggga tcagggtgat cagcttttgg ccccgcaggt tatagacttc
      481 caggcccacg ggtgaagcct ttggcagggt atactgaaac aaaacagagt cctcaaaggg
      541 attggggtat accgataagc ggatcggaga gggagtctgc tggggatcgg cattgggggt
      601 cgtgccccac agttggccca gcaggaagcc attgacctgc tgcgcgttac catggcggtg
      661 ggagttgtcc aggatggcgt agtgatctcc cacggcgggt aggttgaaag tggccgtcga
      721 acccgctgaa gctgtctgag ccagatacgc gtcgcaggtc tgcatcacat cccagaagat
      781 atagttatcg gcatccgtga ccaggagatt gagctctccg ggctgggcta ccggctgcaa
      841 gaaatcagga taatctccca ggctgaagat gtcgtcccag gtgacgcggc cagagatgta
      901 gtagcccggc acctgatact gagtttgaaa ccgtctgagg ggatcaggca cactgggagg
      961 ctcgacggga ttgatggtga taactggcag ggaaccgggg atttccatct caaaatagtg
     1021 caccgccccg tccgccacat tctgaaccag ctccgaatag gtacctgcaa tgggaggata
     1081 gatgccaaag gaggtttccg ctctggcata atagctgcgg ttttctccca ccgggaagct
     1141 caccaagcct tggttgtctg taaaacccac catatcggtc ttcatgccgc cttcctggat
     1201 ggcgaggatc actctggcac catccacggg acgatcttcc tgatctttga ccatcaggtt
     1261 gatctgtgcc aggccgctgg agtaagtgcc tgtcacggaa gccagatagc catcgccgcg
     1321 aatctcgaac acgctgccga aaaccttgcc ccagccctgc tcatagacca tgggaacgtc
     1381 gatgtagcca ttcaccggtt cccacatggt ccactcttca tcccaaaact cgttccaggt
     1441 gtgatcggtg gacatggaga ggatgctggt gcatggaatc aaagcagttc tggcagcggc
     1501 agcggtaaga tcggcatatt ctccgcaacg gccaaagtgc ttgcgataga tgcgcgccgg
     1561 ttgatgagga cgctcgctgt tggaagtgaa actcatgctg gcgtttatcc accactgcaa
     1621 agcccggatg gcgtcaggac ccctgccgct gagatctctg agggtctgaa tctggaccag
     1681 ggtatctgcc atagcgggat agccgtcatc cggttcgttg taaagcatgg cgcgccagaa
     1741 ctttccagtg tccggacccg tagcgttgtt ggtgtggctg ctgttgtttt ccacgatgct
     1801 gggatcgacg aaagcgggaa tctcatcggt gagcttgggt aacactatat accagtagta
     1861 gatatctttg gggacagcga cgctgcgaat ggtgccgtcc gcgtctttct tggtatagct
     1921 ggttgtggac caataatccg ctcccgttgt ggaggacccg gtatcgatca cctccacgta
     1981 gggcaggtct gcggccgcac tgtaaatccg ctgggcattg agagtgaaaa gctgaggcca
     2041 ggcgattgag gaattgaggt attgagggga ggaaacggcc acaccaaacg cgatctcgtc
     2101 cacataagga tcgctggcgg agttgataag accggcaaac agtagctgac gatcctgatt
     2161 gaggcagctg agcgtatatt ccagcctggg cttgatccag acaggggctt tggccacggc
     2221 gttgcgagcg gcgggaagca gatttccaaa gctgcggtaa tagctaaagt catctcccgc
     2281 ggcaaaatac tggatgccca tgtgttcgcc ggccggcagg atgctgttca gggaaaggga
     2341 atcgcagctc acccattgct ctctgtccat cctgcctggg atggcaggag cataattggg
     2401 atggcgaagg cggaactgat tgaggcccag atcggtaagc ttatcataga cggttgttcc
     2461 acagggaatc tcagcctggg tagtgcctct gggggtggcg ctcaaatagc tgattagcag
     2521 gcacagaatc gtgatgatca ggctcttttg catgatcact ccttatgatg taaagaatag
     2581 tgactgtgga gcttacttgg tgcgaacgag gcgaaatccc acgtattgga caccgcggtt
     2641 gggatttccc ttttcccggt agaggatatt gaggttgcgg ccttcgccgt tcatgacact
     2701 gccgccccgg accactcttt gggtgccggt ttcgggaccg gtgggattga tgaatttctg
     2761 gatggtgcgg gtgt
//
LOCUS       DAOSFN010000204         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_35211_length_2753_cov_10.187917, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000204 DAOSFN010000000
VERSION     DAOSFN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35211_length_2753_cov_10.187917"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1101)
                     /gene="gap"
                     /locus_tag="PLO35_09505"
     CDS             complement(103..1101)
                     /gene="gap"
                     /locus_tag="PLO35_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOH79944.1"
                     /translation="MINVAINGFGRIGRLVLRAIIKYHPDINVVAINDLTDAKTLAYL
                     FKYDSVHKIFAGEVSHTDNSIIVNGREIRIFAEKDPEVLPWKDLGVKVVVESTGIFTS
                     KEKASKHLRAGADKVILTAPAKDQVDATVVMGVNHLELKPEHQIVSNASCTTNCLAPV
                     AKVLHDNFGIVGGLMTTIHSYTNDQRILDLPHSDLRRARAAAMSMIPTSTGAAKAIGL
                     VIPELMGKLDGVAIRVPTPDGSLVDLAVNVERETTKEAVNAAMKAAATGPMKGFLMYS
                     DEPIVSIDIVGNHHSSIFDSLTTYVKGNMVKIFSWYDNEWGYSCRVVDLLDYMSKL"
     gene            complement(1113..2087)
                     /locus_tag="PLO35_09510"
     CDS             complement(1113..2087)
                     /locus_tag="PLO35_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOH79945.1"
                     /translation="MFLTGKQMGEVFLKAKRMRFGIIASNIVFDTQIRAIVQGYNAVG
                     SDGLMQMSSGAVKYAAGASQDMKAGARMISTMIRTFADQFPKSGIGLHVDHATPNYFD
                     FLVYCIENQLVTSVMIDASKEALAENIRVTKEVVEVAHKHGIIVEGEIGHIKGTEDEI
                     VSETELYTHPEEALEFVKKTNVDLFAASVGTNHGVSKGENIVLRLDLIKEIDALLIAN
                     GVERGLVLHGASGLTDEQQITAIANGVVKINKDTHYQMDMAQAIQSYWEQEKYAIVCP
                     PDVDPAAYVPNKSKFDPRKWLAKGEEKMQKTVEGFCLVTGSANNSILM"
BASE COUNT          564 a          689 c          810 g          690 t
ORIGIN      
        1 aatctcacct gcgaagcatc tcacatacgc agtatctcac ctgcgaagca tctcacatac
       61 gcagtatctc acatacgcag tatctcacat ggcggagcca tctcacagct tgctcatata
      121 atccagcagg tcaaccacgc ggcagctgta gccccattcg ttgtcatacc agctgaatat
      181 cttcaccata ttgcctttca cgtaggtggt gaggctgtca aagatggagg aatggtggtt
      241 gcccacgatg tcgatgctca cgatgggctc gtctgaatac atcaggaagc ccttcatggg
      301 acccgtcgcg gcggctttca tggcggcgtt gacagcttcc ttggtggtct cgcgttccac
      361 gttcacggcc agatcgacca atgaaccgtc cggagtggga actctgatcg ccacgccgtc
      421 cagtttgccc atcagttcag ggatgacgag accgatggcc ttggcggcac cggtggaggt
      481 ggggatcatg ctcatggcgg ctgctctggc cctgcggaga tcgctgtggg ggaggtccag
      541 aatgcgctga tcgttggtgt aggagtggat ggtggtcatg aggccaccca cgatgccaaa
      601 gttgtcatgc agcaccttgg ccacgggggc caggcagttg gtggtgcagg aagcgttgga
      661 aacgatctgg tgttccggtt tgagctcaag gtggttgaca cccatcacga cggtggcgtc
      721 cacctgatct ttggccggag cggtgaggat caccttgtcg gcgccagctc tgaggtgttt
      781 ggaggccttt tccttgctgg taaagatgcc ggtggattcc accacgactt tcacgccgag
      841 gtctttccag ggcaggactt cagggtcttt ttcggcaaag atgcggattt cgcggccgtt
      901 gacgatgatg ctgttgtcgg tgtgggaaac ttcgcccgcg aagatcttgt ggacgctgtc
      961 atacttaaag aggtaggcca gggtcttggc gtccgtgagg tcgttgatgg ccaccacgtt
     1021 gatatccgga tggtatttga tgatggcgcg caggacgagg cgtccgatgc gtccaaagcc
     1081 gttaatcgct acattgatca ttatttatct ccttacatca gtatgctgtt gttggcgctg
     1141 ccagtgacca ggcaaaagcc ctcgacggtc ttctgcatct tctcttcgcc cttggccagc
     1201 catttgcgcg gatcgaactt ggatttgttg ggcacataag cagcgggatc gacgtccgga
     1261 ggacagacga tggcgtattt ttcctgttcc caatagcttt gaatggcctg ggccatatcc
     1321 atctggtagt gggtgtcttt gttgatcttg accacgccgt tggcgatggc ggttatctgc
     1381 tgttcgtccg tgaggccgga agcgccatgc aacacgagtc cgcgctccac gccgttggcg
     1441 atcaggagcg cgtcgatctc tttgatgagg tcgagccgca gcacgatgtt ttcacctttg
     1501 gagacaccgt ggttggtgcc cacggaagcg gcaaagagat ccacattggt ctttttgaca
     1561 aactccagtg cctcttcggg atgggtgtaa agctcggttt cagagacgat ctcatcctcg
     1621 gtgcctttga tatgaccgat ctcgccttcc acgatgatgc catgtttgtg ggccacttcc
     1681 accacttctt tggtgacgcg gatgttctca gccagggctt ctttggacgc gtcgatcatc
     1741 accgaggtca caagctggtt ttcgatgcag tagaccagga aatcaaagta gttgggtgtg
     1801 gcgtgatcta cgtgtagccc gatcccgctt ttgggaaact gatcggcaaa ggtgcggatc
     1861 atggtggaga tcattctggc cccggctttc atatcctgag aagcgcccgc cgcgtatttg
     1921 acggccccgg agctcatttg catcaggccg tctgaaccca cggcattata gccctggacg
     1981 atggcacgga tttgagtgtc gaagacaata ttggaagcga tgatgccaaa gcgcatccgc
     2041 ttggccttga ggaatacttc ccccatctgt tttccggtta gaaacacgtt atacctcctc
     2101 tggtaatagg atttctatac ttctgacacg ataagccaga gaggggttga cgtcaaggga
     2161 aaaagtgaga gctgcgctgt gagagctttg ctggtgagag ctctgcgagc tggtgagagc
     2221 gttccgctgg cgagagactg cgtatgggag tactgcatcc ttccttactc atgtatcctg
     2281 tggccctcca tagctccccc agagcatacc gaacaaagtt gggggtgatc tggggatgat
     2341 tcacgcggac gcaggaataa gggttaaggg cgaagtagcg aggagtggct aagtagcttt
     2401 ggattaacct gttatatgga gtgtgtgttt gtgctgctga ctacggttaa cggcgctttg
     2461 agtcccatcg ggacgacagt ttagttagat acgcgcaatc cagcgccaat ttgagtccca
     2521 tcgggacggc agtttagatt ggcatagcac tgtgagacag tgggaactgg cgcgactttg
     2581 ccatctgaga taccgttcct gatgaggtct tgtgatacaa tcagtacatg agagtcagta
     2641 atcttgccag tgtgctggtt gaagtccaag aggcccgcaa actcgttgtc atgtaaacac
     2701 ttgtcccggc ctcttgaact ccaacggggt cagtacatga gagtcagtaa tct
//
LOCUS       DAOSFN010000205         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_35495_length_2738_cov_6.972046, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000205 DAOSFN010000000
VERSION     DAOSFN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35495_length_2738_cov_6.972046"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(358..1356)
                     /locus_tag="PLO35_09515"
     CDS             complement(358..1356)
                     /locus_tag="PLO35_09515"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004339150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOH79946.1"
                     /translation="MMSKIALTGIKPTGIPHLGNYLGAIKPALQLSETCEARYFIADY
                     HALNSTKDANSIRQMTLEVAAAWLAAGLDPNKVLIYKQSLVPQTFELLTMLLAFTPKG
                     LMNRAHAYKAMLQSNAENGKDPDDGVNMGLFTYPVLMAADILLFDTDYVPVGKDQFQH
                     VEMAVDIAQSFNYVYGQECFKLPEPMTTPTSHTLPGLDGRKMSKSYDNTIPLFCPEKQ
                     LRKLVMRVITNSQSVEEPKDPETCTIFNIYRNFATDQQIEALRQRYLAGGMGWGHAKQ
                     ELFEVMNAALSPMRTRYDELMANPHTITSILEEGSAKARILAQKKIGYLRSVIGMD"
     gene            complement(1358..2653)
                     /gene="rimO"
                     /locus_tag="PLO35_09520"
     CDS             complement(1358..2653)
                     /gene="rimO"
                     /locus_tag="PLO35_09520"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOH79947.1"
                     /translation="MKTYYLESLGCAKNTVDSEVFARILEDAGLAPSETPEGADLVLV
                     NTCAFLRESLAEVDNVLSELAEYKKDNSIKQLWVTGCLMNRAAEEFQELFPEVDAWIG
                     LKDFDGMAALLKTRYSGSGSRLDLQAGCHAYLRISDGCSNHCSYCTIPSIRGEHHSVP
                     IEALISEAQALASEPSRIVKELVVIAQDTCSYGLDIYGRKALPELLERLHEIDGVEWI
                     RVMYMHPDHFETSWLPLWNKLPKLLPYFEIPIQHCCDDILKAMGRHKGEAELRQLFAD
                     IRAALPEAVLRTTLISGFPGETKAHHDRLKSFVKDVRFMYMGNFVFSPEEGTPAELMD
                     CQVSDSVAEKRRVQLLNLQNKINLQLMKSLLETKVDVLVENEAIDDEFSASGRTWFQA
                     PEVDGMIWIEGSNPTPGQIIKVNIDSAIGYELFGAQEDK"
BASE COUNT          598 a          679 c          774 g          687 t
ORIGIN      
        1 cgaagcactg cttgaagtcc aaagccccgt caggtataga gataaacgta taatccattt
       61 aggggctttg aactccaaga cggccaaggt ggtatttttc acaagctctg aagccagtga
      121 tccggttacg tgcaacagtc tcacattgtc aatcccgcta aggagactgt gtgaaaatgg
      181 ggtgtattga aagaattgta gttgctagat aaggggttat ccataagagc ggcatataga
      241 taaacactga cttttacatg aaaggcctaa cagtcattct cccggagggg gaatccagac
      301 agcatagact cactgcaagg ctcggcctcg catgcagacc cctgcgccag atctcactta
      361 atccattcca atcacgcttc tcagatagcc tatcttcttc tgggccagta ttctggcttt
      421 ggcagacccc tcttccagga tgctggtgat tgtatgggga ttggccatca gctcgtcata
      481 tcgggtccgc atggggctca aggcagcgtt catcacttca aagagctcct gtttggcatg
      541 gccccatccc atcccaccgg ccagatatct ctggcgcagg gcttcgatct gttgatcggt
      601 agcgaaattg cggtagatgt tgaagatcgt gcaggtttcc gggtctttgg gttcttccac
      661 actctgggag ttggtgatga cgcgcatcac cagcttgcgg agctgttttt caggacagaa
      721 gagtggaata gtgttgtcgt agctcttgct catcttgcgg ccgtcaagac caggcaaggt
      781 atgagaagtg ggggtggtca tcggttctgg cagtttaaaa cattcctgcc catagacata
      841 gttaaagctt tgggcaatgt cgacagccat ttccacatgc tgaaactggt ctttgcccac
      901 tggtacataa tcggtatcaa agagcaggat atctgccgcc atgagaacgg gataggtaaa
      961 gaggcccatg ttgaccccgt cgtccggatc tttaccgttt tcggcattgg actgcagcat
     1021 ggccttgtag gcgtgagctc tgttcatcag gcctttggga gtaaaggcca gcagcatcgt
     1081 gagcagctca aaggtttgag gtacaaggga ttgcttgtag atcagcacct tgttgggatc
     1141 cagtccggca gccagccagg cggctgccac ttcgagggtc atctgacgga tggagttcgc
     1201 gtccttggtg gaattgaggg cgtggtaatc cgctatgaaa taacgcgctt cgcaagtctc
     1261 ggaaagctgc agggcgggtt tgatggcgcc cagataattg cccagatggg gtattcccgt
     1321 gggtttgatg ccggtgagtg ctatcttgct catgatgcta tttatcctct tgggcgccaa
     1381 agagttcgta gccgatggcg ctatcaatgt ttactttgat tatctgtcca ggagtgggat
     1441 ttgagccctc gatccagatc atgccgtcca cctcaggtgc ctgaaaccag gttcttccgg
     1501 aggcagagaa ctcatcgtcg attgcttcgt tttccaccag cacgtcaacc tttgtttcaa
     1561 gcaggctttt catgagctgg aggttgatct tgttctgaag attgagaagc tggacgcggc
     1621 gcttttcagc cacggaatcg cttacctggc agtccattaa ctcggctgga gtaccctctt
     1681 cgggtgagaa aacaaagttg cccatataca tgaagcggac gtctttgaca aagcttttca
     1741 gcctgtcatg atgggctttg gtctctccag ggaagccaga aatgagggtg gtgcgcagca
     1801 cggcttcagg cagggcagct cttatgtctg caaaaagctg cctgagttct gcttcgcctt
     1861 tgtggcgtcc catggctttg aggatgtcat cgcagcagtg ctggatgggt atctcgaagt
     1921 agggcagcag cttgggaagc ttattccaga gcggcagcca ggaggtctca aagtgatcgg
     1981 gatgcatata catcactctg atccattcga cgccgtcaat ctcgtgcaga cgttccagca
     2041 gctccggtaa ggctttgcgg ccatagatgt ccaggccgta ggaacaggtg tcctgggcga
     2101 tcacaaccag ctctttgaca atcctggatg gctctgatgc cagggcctga gcttcggata
     2161 tcagggcctc gataggaacg ctgtgatgct ctccccggat ggatgggatg gtgcagtagc
     2221 tgcagtgatt ggagcagccg tcgctgatcc taaggtaggc atggcagcct gcctggaggt
     2281 ccagtctgct gcctgaaccg gagtatctgg tcttgaggag ggccgccatg ccgtcaaagt
     2341 ctttaagacc tatccaggca tccacttccg gaaacagctc ctgaaactcc tccgcggcgc
     2401 ggttcatcag gcaaccggtc acccagagct gcttgatgct gttatctttc ttatattcag
     2461 ccagttcgct gagcacgttg tctacctcgg ccagagattc tctcagaaag gcgcaggtat
     2521 tcaccagcac cagatcggcc ccttcgggtg tttccgaagg ggccaatccg gcatcttcaa
     2581 ggattctggc aaagacctcg ctgtccacag tatttttggc gcagccgaga ctttcgaggt
     2641 agtaggtttt cacttaaggg gatgaatgaa cagccccgag cggagtttgg gctcgaacca
     2701 ggtggatttg gggggcatga tctcaccggc gtcggcaa
//
LOCUS       DAOSFN010000206         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_35497_length_2738_cov_5.760716, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000206 DAOSFN010000000
VERSION     DAOSFN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35497_length_2738_cov_5.760716"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1332
                     /locus_tag="PLO35_09525"
     CDS             154..1332
                     /locus_tag="PLO35_09525"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH79948.1"
                     /translation="MIFKLAFRNVIGYGWRSVINVFIIALVLVGLVWMISLWASWINL
                     AKTQQQDWEYGSGTMRARAYDPLDMFSWEKAYAPVPSEARPHIDSGLVVPVLFSPGAL
                     YSHGRMTNVMIKGIPAAQKVLKIPSATLENHPDHSIPALLGKAMAKSARLQEGDIFTI
                     RIKDAAGAFNTLDLTVAQVMDCPVPSLDVNTVWMDLEALRQAKDLPDRATVLVLADSR
                     LSSIPDKDFRYIDRSEFFADIDQMVKTKAGGQSFFFMMLIGLAMLAVFDTQALAVFKR
                     RKEIGTLSALGFTKGQIIRIFTLEGSIYVLLAMLAGAVLGLPLFWYFGTHGLTLPGGS
                     EGFGVQGFDEPLKFVYPPHLILLTLVFVFGLTVFVSWLPARRISRLNPTDAIRGRDHA
                     "
     gene            1325..2596
                     /locus_tag="PLO35_09530"
     CDS             1325..2596
                     /locus_tag="PLO35_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH79949.1"
                     /translation="MLKFLIKGLFRDRHRYLFPLIMVGVGVWMMVFMLSFMNGYTESF
                     IRQNAGFETGHLKVVTRAYAEMPDLRPYDLGLLDYQDGLKAWKRDYPQFDWLERIHFG
                     ALMDVPDANGDTREQGNIVGFGVDLFSSRLERRYMNLDKALVQGRIPARPMEALLSDK
                     AFRKLSLRLGDTITLIASDINGAMAFRNFRVSGTIDFGMEALDRGAVIADLSDVRQML
                     DMENGAGEILLLFKDGKYHPREVARVRDTFNARYSDPQDEYSPVMRAMSDQNNFGYLM
                     NMLQSILGMVSAVFTFILAVVLWNSGLMNGIRRYSEFGVRLAVGESKWHIYKSLLAEA
                     LVLGLTGSLIGILLGLVFSAYFSTHGLDMRVFSRNSSLMAEDMIYTALDLRTALAGFI
                     PGVLSNLLGAALAGIAVFKRQTARLFNELEV"
BASE COUNT          549 a          793 c          780 g          616 t
ORIGIN      
        1 accggctaaa gccagtgcta cgaccggcta aagccagtga tacgaccggc taaagccagt
       61 gttacgaccg gctaaagcca gtgttacgta ccggctaaag ccagtgttac aacaatttaa
      121 gacagatatt tgtatgcaaa aggcaggcct gcaatgatct tcaagctggc gtttcgaaac
      181 gtgatcggct atggatggcg cagcgtgatc aatgtattta tcatcgcgct ggtcctggtg
      241 ggactggtgt ggatgatctc gttgtgggcc agttggatca atctggccaa gacccagcag
      301 caggattggg aatacggctc cgggaccatg agggcgcgcg cttatgatcc gctggacatg
      361 ttcagctggg aaaaagccta cgctcccgtc ccctctgaag cccgccccca tatcgattcc
      421 gggctcgtgg tccccgtgct cttttcaccg ggagcgctgt attcccatgg ccgcatgaca
      481 aacgtgatga tcaaaggcat tccggccgcc cagaaagtgc tcaaaatccc ctccgccacc
      541 ctggaaaatc atcccgacca ttccatcccg gccctgctgg gcaaggccat ggccaaaagc
      601 gcccggctcc aggaaggcga tatattcacg atcaggatca aagacgccgc cggggccttc
      661 aacacactcg atttgacggt ggcccaggtg atggactgcc ccgtcccctc tctggacgtc
      721 aacaccgtct ggatggacct cgaggccctg cgccaggcca aagatttgcc ggatcgggcc
      781 accgtcctcg tcttggcgga ttcccgcctg agttccattc cggacaaaga cttccgctat
      841 atcgaccgga gcgagttctt tgccgatata gatcagatgg tcaagaccaa ggccggcggg
      901 cagagctttt tctttatgat gctgatcggc ctggcgatgc tggccgtgtt cgacacccag
      961 gccctggccg tgttcaagcg ccgcaaggag atcgggacgc tctccgctct gggcttcacc
     1021 aagggccaga tcatccggat tttcaccctg gaaggctcca tatatgtgct cctggccatg
     1081 ttggcgggag cggtgctggg cttgccgctt ttctggtatt tcggcaccca tggcctcacc
     1141 ctgcctggag gctcggaagg ctttggagtg caaggctttg acgaacctct caagtttgtc
     1201 tatcctcccc atctgatcct gctcaccctg gtctttgtgt ttgggctcac ggtgtttgtg
     1261 agctggctgc cggcccgcag gatatccagg ctcaatccca ccgacgccat cagagggaga
     1321 gaccatgctt aagtttctga taaaaggtct tttccgcgat cgtcaccgct atctctttcc
     1381 cctcatcatg gtgggcgtgg gggtctggat gatggtgttt atgctcagct tcatgaatgg
     1441 ctacaccgag agttttatcc gtcagaacgc gggttttgag acgggccacc tcaaggtcgt
     1501 gacccgcgct tatgctgaaa tgccagacct caggccctat gatctgggcc tgctggacta
     1561 tcaggacggg ctaaaggcct ggaagcggga ttatcctcag ttcgactggc tggaacgcat
     1621 ccatttcggc gctctgatgg acgttccgga tgccaatggc gacacgcgtg agcagggcaa
     1681 tatcgtgggc tttggagtgg acctcttttc ttcccgcctc gagcgtcgct atatgaatct
     1741 ggataaagcc ctcgtccagg gcaggattcc agcccgtccg atggaagcct tgctgagcga
     1801 caaggctttt cgcaagctga gcctcaggct gggagacacc atcacgctga tcgcctcaga
     1861 catcaacggc gccatggcgt ttcgcaattt ccgggtcagc ggcaccatcg attttggcat
     1921 ggaagcgctg gaccggggag ccgtgatcgc cgatctttct gacgtacgtc agatgctgga
     1981 catggaaaac ggcgcgggag aaatcctgct cctcttcaaa gacggcaaat atcaccccag
     2041 ggaagtggcc agagtccggg acaccttcaa cgcgcgctac tccgaccccc aggatgaata
     2101 ctcacccgtg atgcgcgcca tgagcgacca aaacaacttt ggctatctga tgaacatgct
     2161 gcaaagcatc ctgggcatgg tgagcgccgt ctttaccttc atcctggcag tggtgctgtg
     2221 gaactccggc ctcatgaacg gcatccggcg ctacagcgag tttggagttc gcctggcggt
     2281 gggtgaatcc aagtggcaca tctacaagag cttgctggcc gaagctctgg tcctgggcct
     2341 cacgggttcc ctgatcggaa tcctcctggg cctggtcttc agcgcttatt tcagcaccca
     2401 cggcctcgat atgagagtct tttcccgcaa ttcatccctg atggccgaag acatgatcta
     2461 caccgcttta gacctccgca ccgccctggc gggattcatt ccgggagtgc tttccaacct
     2521 cctgggagcc gcgctggcag gaatcgccgt gtttaaacgc cagaccgcga ggctgtttaa
     2581 cgagctggag gtgtgagggg cgttacgcgt gatgcgtgat gcgttatgcg ttatccgtta
     2641 cgcgttatgc gggcgcccat ggatacactc gttgcatttg ccaggccgct cccgttggag
     2701 ttcaagcggc cacgataaga gtatatatgg cagcgagt
//
LOCUS       DAOSFN010000207         2716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_35918_length_2716_cov_8.355881, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000207 DAOSFN010000000
VERSION     DAOSFN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35918_length_2716_cov_8.355881"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            576..1874
                     /locus_tag="PLO35_09535"
     CDS             576..1874
                     /locus_tag="PLO35_09535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH79950.1"
                     /translation="MRFRNFSPVERHTATLLLWGALFNGVVQSLGQTQDIIARKALHA
                     QDWQLMLMTMIWPISNFFSIWWGRAFENSCHKSRLIFIAGIIGRLSLVAAVWIVTMNQ
                     YLLLLALLFSSNSLLIPAQNSIYQRNITKHWRARLYGYTISLGMLVSVIFTYGAGRLL
                     DVREESFRWILVVTGICGFITSALWASIRIQEPLSQKSCEKPRFKDLALDPIRRTLTL
                     LKENKPFAAFERSFSIYGMGFIMMQPIIPIYMVDKLNLSYTNNFLAKGVLSQIGMLLL
                     SPMIGKLHDRLHPFRFISASFALLMVFPLLFISSSLWVGSPIAVVIVFIAYLIFGIAM
                     TGVNIAWNMSSIFFAGKDDASMYQSVHVTMTGIRGLIAPVLGFTLLKVFNISVVFFVA
                     AGFLAWASLISLRDHRRLAKQLPEPRPEDLEELSDLPKPL"
     gene            2107..2430
                     /locus_tag="PLO35_09540"
     CDS             2107..2430
                     /locus_tag="PLO35_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOH79951.1"
                     /translation="MKYTVQTKEKTMTKADLVKIISENTGIIRKDVAVVVDALLQSVK
                     DSMSAGQHIEVRGFGTFKLKTRKPRVGRNPKTDEKVPVPARTVPTFKFSREFKNAVID
                     IKVAE"
BASE COUNT          629 a          787 c          654 g          646 t
ORIGIN      
        1 gttccgctac gctgcacagg gggctatgac attgactcac ccctgcgggg ttgatggtcg
       61 cacaggctct tcagccggtc gttgtgccgg ctttagccgg taccacgcga tccagactct
      121 gagtgataca ccattgatgt gaaagcagat agtattttat cacgcaccga ctaaagtcag
      181 tgctacgacc ggctgaagcc agtgttacga tcatgtgcat cggtctccgg caatatccat
      241 tccagcacca tgaggtggtg aatccgtgaa aatcagttca atctgcgtca tctgtgttct
      301 attctccctc caggcccaaa acccctaacc agaataagcg taaacattgg acttcatcgg
      361 agcgtattat atggcgggtg tgctggttga agtccaagcg gcccgcaaac tcgttgtcat
      421 gcatagcttt atcgtggccg catgaactcc aacaggaccg gaaggcttcg cataacgcat
      481 aacgcgtaac gacctaaaac cctacaactt cacatgcccc ttgacagaaa aaccgccctc
      541 aaagcttttg ctctcaaaca attctgagga gaaggatgag attcaggaat ttctctccgg
      601 tcgaaaggca caccgccacg ctgctcttgt ggggcgcgct tttcaacgga gtggtgcaaa
      661 gccttggcca gacgcaggac atcattgccc gcaaggccct gcacgctcag gattggcaac
      721 tgatgctgat gacgatgatc tggccgatct caaacttctt ttccatctgg tggggcaggg
      781 cttttgagaa cagctgccac aaatcccggc tgatcttcat cgcggggatc attggccgcc
      841 tcagtctcgt ggccgccgtc tggatcgtga ccatgaacca gtatctgctg ctgctggcct
      901 tgctcttttc ctccaattcc ctgctcatcc cagcccagaa cagcatctac cagcgcaata
      961 tcacaaagca ctggcgggcc cgcctttatg gctacacgat cagcctcgga atgctggtga
     1021 gcgtgatctt tacctatggg gcggggaggc tgctggacgt ccgcgaggag agctttcgct
     1081 ggattttggt ggtcacaggc atctgtggat ttatcaccag cgcgctctgg gcctcgatcc
     1141 gcatccagga acccctcagc cagaagagct gcgaaaaacc ccgcttcaaa gacctcgctc
     1201 tggaccccat ccgccgcact ctcactctcc tgaaggaaaa caagcccttc gccgctttcg
     1261 agcgcagctt ttccatctac ggcatgggtt ttatcatgat gcagcctatc atccccatct
     1321 atatggtgga taagctaaac ctctcctaca caaacaactt cctggccaag ggagtgctct
     1381 cccagatcgg catgctgttg ctctctccca tgatcggcaa actgcacgac cgcctgcatc
     1441 ccttccgttt catatccgcg tccttcgcgc tcttaatggt tttcccgctg ctgtttatct
     1501 catcctcgct gtgggtgggc agccccattg ccgtcgtgat tgtctttatc gcctatctca
     1561 tcttcgggat cgccatgaca ggcgtaaaca tcgcctggaa catgagttcc atcttctttg
     1621 ccggcaagga cgacgcttcc atgtatcaga gcgtgcacgt caccatgacc ggcatccgtg
     1681 gtttgatcgc ccccgtgctg ggtttcacgc tgctcaaggt cttcaatatc agcgtggtct
     1741 tctttgtggc ggcgggcttt ctggcctggg cctcactgat cagcctgaga gaccatcgcc
     1801 gtctggccaa acagctgccg gaaccccgtc ctgaggacct cgaagagctc agtgatctcc
     1861 ccaagccgct ttgacctgat aaaagcgcgg acgatgccat tctcccacaa gctcctcccg
     1921 cagacccgtt taagcccctg cccgggtttc aaggtgaagt ctctgattgg cgtttttccc
     1981 tcatggctaa aggatttcag gcatacgagg ccgacggcat tcaaacgcct cccagttttt
     2041 tttcttgaca taaaagctaa gatttttgtt ttgagtactt tgttaccaaa cacgaaatca
     2101 acctgattga aatacacagt acaaaccaag gagaaaacaa tgactaaagc agatttggtg
     2161 aagatcattt cggaaaacac cggtatcatc cgcaaagacg tagctgttgt agtggacgct
     2221 cttctgcagt ccgtcaaaga cagcatgagc gctggccagc acatcgaagt gcgcggattt
     2281 ggcaccttca agctcaaaac ccgcaagccc cgcgtgggcc gcaaccccaa aaccgacgag
     2341 aaagtgccag taccggcccg taccgttccc accttcaaat tctcacgtga attcaagaac
     2401 gcggtgatcg atattaaagt tgcggagtaa ggtagcgacc aatgccctgc ggtaagaaac
     2461 gtaaaagaca caaaatcgcc acccacaagc ggaagaaacg cctccgcaag aatcgtcaca
     2521 aaaagaaata agcttgtctg agggccgcct gatggcggcc cttagctttt gtagggttac
     2581 gcgtgatgcg ttaagcggta tgcggtatgc gatctccacg tgtgcctcag ttcccattct
     2641 gtgttgctct cgtatctctt tcctctgttt taaaaaagaa tagaacacag atgacactga
     2701 ttaaactgat ctacgc
//
LOCUS       DAOSFN010000208         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_36220_length_2700_cov_8.977316, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000208 DAOSFN010000000
VERSION     DAOSFN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36220_length_2700_cov_8.977316"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..1717)
                     /locus_tag="PLO35_09545"
     CDS             complement(275..1717)
                     /locus_tag="PLO35_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79952.1"
                     /translation="MKKTFILLFCFTSLMISSQTVTFRNENMMMEVGPAWMFNYNLTE
                     AFNEDFHDSAAHPQWVYETLSYNWLSYPYTVNSTPLWTYKSSSKLNAYASFPPLAGVA
                     VPDTTIKFFFGERGSEDYLKNCAFSHINTLNADWDWDTMGQAGDTRLYTGATIYVKSN
                     VWGAAQTLLRLNNCSMTTTSAYPGPIGSNEPIIGYGWGTLDPVNGDPDWIDEWTNDTG
                     QLQFTFSSSSAQVQDNFGYYSSDITLSIPTFKRDLVSAPDVLTPYTLDAIDTHWASLT
                     VESGSYWGDDLSTGIIHTSSICKYYSPSIGNYPAHIVNHYDGAYWEIGNTFNHSLSTL
                     LFDFSDVPGVSAPENLRVLRRQNGYGVSWVDVNAQLISTNPLRMQISGIDIMGQYCLA
                     STGGNNLAIDSPDNVILIRDASLTPDLRLSWNEVNGASQYKVYSSSSPDSPDDEWTLE
                     ATLAHPVTEWSSSSADPRRFYHVKALKADW"
     gene            2266..>2700
                     /locus_tag="PLO35_09550"
     CDS             2266..>2700
                     /locus_tag="PLO35_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79953.1"
                     /translation="MKVTLKHRLRSYQGECDDLVYYYNRRTGTTLARRYVKPRYSSAN
                     HKLGQGARHLRALNPSPLYLQDLKLYIELHNSLRDPEDGLLNNRWALYTKVMYAQAKA
                     LGLSVTQLTPELIEAQQLPCRCVKTAVEAGFLEVVEGYERLTN"
BASE COUNT          651 a          718 c          688 g          643 t
ORIGIN      
        1 gtttgggaca ccggatctat ctaaactgtc gtcccgctgg gactcgtaaa gaataccacg
       61 ataatcagtt tttttaacaa gctcttcagc cggtttttgc actgacttca gtcggtgcgt
      121 gcaacaagcc tgcctgcttt catgacaatg aataccgcgt tatggccaaa ggttttggtg
      181 ccggttaaaa cctgcacaac gtccggctaa agccagtgat accatataac gccaagctgt
      241 tcacccttca cctctcacca cccagctccc gatcttacca atccgccttc agcgctttga
      301 catgataaaa cctgcgggga tcggctgaac tgctgctcca ctctgtaacc ggatgcgcca
      361 gcgtggcttc cagagtccat tcatcatccg gagagtccgg gcttgaggat gaatagacct
      421 tatactggct ggcaccgttc acctcattcc agctcagtcg cagatctggc gtgaggctgg
      481 catccctgat cagaatcaca ttgtcgggcg agtcaatcgc caggttgttg ccacccgtgg
      541 aggccagaca gtactggccc atgatgtcga tcccggaaat ctgcatgcgc agcgggttgg
      601 tgctgatcaa ctgggcattc acatccaccc agctgactcc atagccgttt tggcgtctga
      661 ggactctcag attttcggga gcgctcactc ccgggacgtc cgaaaagtca aagaggagag
      721 tggaaaggct gtgattgaag gtgtttccaa tctcccaata agctccatca taatgattta
      781 cgatatgagc agggtagttt ccgatgctag gggagtaata cttgcagatg ctgctggtgt
      841 ggatgatgcc ggtgctcaaa tcgtcgcccc aatagctgcc gctctccacc gtcagtgaag
      901 cccagtgggt atcgatcgcg tccagagtat atggagtcaa aacgtccggg gcggacacca
      961 ggtcccgttt gaaggtaggg atggaaagtg tgatatccga gctgtagtag ccaaaattat
     1021 cctgcacctg ggccgaggaa ctggaaaacg tgaactgcaa ctggccggta tcgttggtcc
     1081 attcatcaat ccagtcggga tcgccgttta cagggtccaa agttccccag ccgtaaccga
     1141 ttatcggttc attcgatcca attggcccgg gataagctga agtggtggtc atggagcagt
     1201 tattgaggcg cagcaaggtc tgagctgccc cccacacgtt ggattttacg tatatggtgg
     1261 cccctgtata gagtctggta tcaccggcct ggcccatcgt atcccagtcc caatctgcat
     1321 tgagcgtgtt tatgtgagag aacgcgcagt ttttgaggta atcctcagaa ccgcgctcgc
     1381 caaaaaagaa cttgatggta gtatccggca cagccacacc tgccagggga gggaatgatg
     1441 cgtaggcatt caatttgctg ctggacttat aggtccaaag cggggtcgaa ttgacggtgt
     1501 aagggtagct gagccagttg tatgagaggg tttcatacac ccattgcgga tgtgcagcgg
     1561 aatcatgaaa atcctcattg aaggcttcag taaggttgta gttaaacatc caggctgggc
     1621 caacttccat catcatgttc tcattgcgaa aggtcactgt ttgggaactg atcatcagac
     1681 tggtgaaaca aaacagcagt ataaacgtct ttttcatcat caacctcggc agattgtttt
     1741 gcgtttcatc ccaagtaatg agcaataact gtcaagatat ttcgtgaatc tgatgccaaa
     1801 ccgcgtcaga aacgataact tgcgcggagt ccaagctcca tcataacagc gatcttcgtc
     1861 cattgctcag ttgaacgggc tcaccaggaa gctggcaatt ggagggattg ggcctcgtcc
     1921 tttgcctcgg gtttctgttc ccgatgtaaa tatttccaaa atcgcgggaa attatcgctt
     1981 tggtcaagtt gctactttct gatttccact caagtatcag ttgttattaa tctgtcttta
     2041 ttgtggttac agccttttgt gtcactctgt atcaggcccc tctctggggt catcacagct
     2101 gtgtcgccct tatatatata gaggcatgtt ttgcgagccg cctcctttac accacctatg
     2161 ccccaccttt acatcaccaa tgcctcggca aaggaggagc ggcagtgagg tgaaagaaag
     2221 gcaaacggag caagcactta acaacctacc aaaaaggaga aaaccatgaa agttaccctc
     2281 aaacaccgcc tgagaagcta ccagggcgaa tgcgacgacc tggtctatta ctacaaccgc
     2341 cgcaccggga ccacgctggc ccgccgctac gtaaaacccc gctattccag cgccaaccac
     2401 aagctgggcc agggtgccag acacctccgc gccctcaatc cctccccgct ctatctgcag
     2461 gacctcaagc tctatatcga gctccacaac tccctccgcg atcccgaaga cggcctcctg
     2521 aacaaccgct gggccctcta caccaaggtg atgtatgccc aggccaaagc gctgggactt
     2581 agcgttacgc agctcactcc ggaactgatc gaggctcagc aactgccgtg tcgatgcgtg
     2641 aaaactgcgg tggaagcggg atttttggag gtggtggagg ggtatgaacg gctgacgaat
//
LOCUS       DAOSFN010000209         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_37907_length_2619_cov_7.145866, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000209 DAOSFN010000000
VERSION     DAOSFN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37907_length_2619_cov_7.145866"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /gene="secD"
                     /locus_tag="PLO35_09555"
     CDS             <1..644
                     /gene="secD"
                     /locus_tag="PLO35_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOH79954.1"
                     /translation="PGGRAQITGSFTTTEANELAILLSKGNLKAPITPISTSIIGATL
                     GADSIKSGTKAGMIGLIAVVLFMLIYYKAAGFISDFVLVFNIGFILAVLTMLGATLTL
                     PGIAGIILTIGMAVDANVLIFERIREELDAGKTPRSAVDAGYKRAVVTVWDANLTTLI
                     AAAVLYKFGSGPIRGFAITLAIGIVGSMFCAIVFVRSILDNFFLTGNKKTMSI"
     gene            655..1635
                     /gene="secF"
                     /locus_tag="PLO35_09560"
     CDS             655..1635
                     /gene="secF"
                     /locus_tag="PLO35_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOH79955.1"
                     /translation="MRLFRNTNIPFVKIRYVAYAISALLLIVSLIAIFTNSLNWSIDF
                     TSGVSAKVNLKAADPKVPNLQVSKLRAVLKRSGFPEAEIQNIGEPENSSFLIKLKSQK
                     DTDEVESKTKDRIIEIIQTNFPEYVKNRDLSREVIEEIYVVGPKVGGELRDQVTLALV
                     IALILMIIYIWFRFELTFGLMAILALFHDVTIIVGAFAITGKEMTFQIIAALLTIVGY
                     SINDTIVIFDRIREDLKIYRKEPVPVIFNRSMNQTLSRTTITSGTTLLSALALFFFGG
                     SVLHDFAFAMVLGVFFGTYSSIFVASNLVIDAYHITHKEKKGIQHLTKKK"
     gene            complement(1904..2374)
                     /locus_tag="PLO35_09565"
     CDS             complement(1904..2374)
                     /locus_tag="PLO35_09565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOH79956.1"
                     /translation="MKITELSTAYAKVHLPAKVVLAVTQKPDGGYNLITLEWFMRTSI
                     QPPMFAISIGHSRFSHDCLEENRFFNLVFPSQQMKAVCSLAGSKSGRDIDKFTETGVK
                     HIPGKLRKLPVLSDAVAVFECEVVTQVRSGDHTIYVGEVKHSWMNEEGELLVYR"
BASE COUNT          594 a          756 c          654 g          615 t
ORIGIN      
        1 ttcctggcgg ccgggctcaa atcaccggca gctttaccac caccgaagcc aacgaactgg
       61 ccatcctgct cagcaaaggc aatctgaaag ctcccatcac ccccatcagc acctccatca
      121 tcggcgccac ccttggcgcg gacagcatca agagcggcac caaagcaggt atgatcggcc
      181 tgatcgccgt agtcctcttt atgctgatct attataaggc cgcggggttc atttcggatt
      241 ttgtgctggt attcaacatt ggctttatcc tggccgtgct caccatgctt ggcgcgaccc
      301 tcactttgcc tggtatcgct ggaattatcc tcacaatcgg catggcagtg gatgccaacg
      361 tgctgatctt tgagcgcatc cgtgaagagc tcgacgccgg caagactccc cgttccgctg
      421 tggatgccgg ttacaaacgc gccgtggtca ccgtgtggga tgccaacctc accactttaa
      481 tcgccgccgc ggtgctctac aagtttggtt ctggtcctat ccgcggcttt gccatcaccc
      541 tggccatcgg cattgtgggc tccatgttct gcgcgatcgt gtttgtccgc tcgatcctgg
      601 acaacttctt cctgaccggc aacaaaaaga ccatgagtat ctgaggagct tgaaatgaga
      661 ctgtttagaa acaccaacat ccccttcgtc aagatccgct atgttgccta cgcgatatct
      721 gctctgctat tgatcgtgag cctgatcgcg atcttcacaa atagcctgaa ttggagcatt
      781 gacttcacca gcggcgtttc cgccaaagtc aatctgaaag cggccgatcc caaggtgcca
      841 aacctgcagg tatccaagct gcgtgccgtc ctgaaacgca gcggcttccc ggaagccgag
      901 atccagaaca tcggcgagcc tgaaaactca tccttcctga tcaagctcaa aagccagaaa
      961 gacaccgatg aagtggaaag caagaccaaa gaccgcatca tcgagatcat ccagaccaat
     1021 ttcccggaat atgtgaaaaa tagagacctc tcccgcgagg tgatagaaga gatctacgtg
     1081 gtggggccca aggtgggagg cgaactgcga gaccaggtta ctctggccct cgtgatcgcc
     1141 ctcatcctga tgatcatcta catctggttc cgctttgagc tcacctttgg gctcatggcc
     1201 attctggccc tctttcacga cgtgacgatc atcgtgggag cttttgccat caccggcaag
     1261 gagatgacct tccagatcat cgcggccctg ctgaccattg tgggttactc gatcaacgac
     1321 accatcgtga tcttcgatcg catccgcgaa gacctcaaga tctatcgcaa agaacccgtc
     1381 ccggtgatct tcaaccgctc gatgaaccag accctgagcc gcaccaccat cacctcgggt
     1441 accaccctgc tcagcgctct ggccctgttc ttctttggcg gcagcgtgct gcacgatttt
     1501 gcctttgcca tggtgctggg tgtgttcttt gggacctact cctccatctt cgtggccagc
     1561 aacctcgtga tcgatgccta ccatatcacc cacaaagaga agaaaggcat ccaacacctc
     1621 accaagaaaa aataagcctg gatttagccc agaacattaa agccgcccta cctcggggcg
     1681 gtttttttgg ggaaaccgga aaggtcatcg tttccggggc cggtatcgtc gatatccaaa
     1741 tcggtgtgtg attcacgatg cgtctatgaa cgcagtcata aagaaccccc aatccctctg
     1801 tttatatacc tctgtttttc tctgtcccaa actccgcttt tctctgggtt ttatctgatt
     1861 acgataacgg cactcgccgg attggaatcc gacgatacga gagtcagcgg taaaccaaca
     1921 gctccccttc ctcgttcatc cagctgtgtt taacctcacc cacgtagatg gtgtgatctc
     1981 cgctgcgcac ctgggtcacg acttcgcatt caaacacggc gaccgcgtcc gagagcacag
     2041 gcagtttgcg cagcttgccg ggaatgtgtt ttaccccggt ttcggtaaat ttatctatat
     2101 cgcggccaga cttggagccc gcgagggagc agacagcttt catctgctgg gagggaaaca
     2161 ccaggttgaa aaagcggttc tcctccaggc aatcatggct gaagcgcgag tggccaatcg
     2221 aaatggcaaa catgggcggc tggatggagg tgcgcataaa ccactcgaga gtgatcaggt
     2281 tatagcctcc atctggcttt tgagtgaccg ccaggactac tttggcgggc aaatgcactt
     2341 tggcgtaggc agtgctgagt tcggtgattt tcatttatca atatctccaa taagcgatat
     2401 ggataaatta gtcaaatatc ctttgggggc aagcgattct atcctggacc gctgacgcgc
     2461 ccttacccgt tgtccgcttg gccctcggta gctcgccgta cccatcccca ataaagttcg
     2521 ggatgggtag gagatggtat aggggggcac gggagccatg agtaagggcc agctcagcaa
     2581 ctgacttcag agcatatgct aaagggcctt ttacagctt
//
LOCUS       DAOSFN010000210         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_37982_length_2616_cov_8.702850, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000210 DAOSFN010000000
VERSION     DAOSFN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37982_length_2616_cov_8.702850"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(453..1196)
                     /locus_tag="PLO35_09570"
     CDS             complement(453..1196)
                     /locus_tag="PLO35_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79957.1"
                     /translation="MIIEQLKTLVEMQKLDDQIGRYRILQLELPKQLSEIVDRVEQAT
                     ATLLAVETEKAELNKKQRAMEGDIKQHGDQARKYATQLSEIKTNKEYKALNSEIAYLK
                     EKISELESQEIELMDLETEIKVRVDEAKKELAAAEKAKRDQEGELRAQIDSLETKIEE
                     TRAQRNKLAATLPNQIIKQYGNMIKNKNNVAVAWNKDGSCGACGFVIRPQIRIELQLF
                     KRLIPCENCGRILIDSSLENQGNEEEASN"
     gene            complement(1199..2020)
                     /gene="nadC"
                     /locus_tag="PLO35_09575"
     CDS             complement(1199..2020)
                     /gene="nadC"
                     /locus_tag="PLO35_09575"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOH79958.1"
                     /translation="MQINDIVREALSEDLGSGDITAAYLDLDPVSSRAFMVAKAPGVL
                     AGVEVAKQVFKTVDPDLKITVYRKDGDQVQPKDEILSIEGKAASILMGERVALNFLQR
                     LSGIATQTRQMVNALEGTGVRLLDTRKTTPMLRLLEKYAVRVGGGFNHRFGLYDMVML
                     KENHIRACGGITEAMRRVKSHNTSYKVEVEVTNPEELAEAVAAGADRVMLDNMDIPAI
                     KAAAKKYRKQVELEISGGVTPENIAKYARAGVHYISSGSITHSYKSLDISLLFEE"
BASE COUNT          577 a          641 c          708 g          690 t
ORIGIN      
        1 gttcaaagcc cctggacaga ataggcgttt atcttcttgc ttgacggagt tttggacttg
       61 cgtgtagcac atcgaagaca tttatctcgc gggcgtcctg gttgaagtcc aagcggccag
      121 caaactcgtt gtcatgcata tatttatcgt ggccgcttga actccaacgg ggcttagtta
      181 gtctctggtt tcggtatttt ttcacaagct cataagccag cgttacgtat gtgcgcagga
      241 tattgagtga gatttcgggg gtgatctaag gagcgaattc tgagccgggg agcgcggtac
      301 ttcagcaaaa gatccgtgag taacaattgt gagcccccac ctggtagtga ggaggatgca
      361 tgacagactc atggccccac gcgacgctgc tcagccctcc gctgagccca gatcagatat
      421 ctgccgtctg aattgtgagc tgattggtag ctttagttgg aggcttcttc ctcgttgccc
      481 tggttttcca ggctggagtc gatcaggatg cgtccgcaat tctcacaggg gatgaggcgc
      541 ttgaaaagct ggagttcgat gcggatctga ggacggatca cgaagccgca ggccccgcag
      601 gaaccgtctt tgttccatgc cactgccacg ttgtttttgt tcttgatcat gttgccatat
      661 tgcttgatga tctggtttgg cagggtagcg gccagtttgt tgcgctgggc gcgggtctct
      721 tcgatcttgg tttccaggct gtcgatctga gcgcgcagtt cgccttcctg atcgcgtttg
      781 gctttttcag cggcggccag ctcttttttg gcctcatcga cgcggacctt gatttcggtt
      841 tcgaggtcca tcagctcgat ttcctgggat tccagctccg agatcttttc tttgagatag
      901 gctatttcgc tgttgagggc cttatattcc ttgttggtct tgatctccga gagctgggtg
      961 gcgtattttc gggcctggtc gccatgctgt ttgatgtcac cttccatagc ccgctgtttt
     1021 ttgttgagct cggccttttc ggtttcgacg gccaggagag tagccgtggc ctgttccacg
     1081 cggtccacga tctccgagag ctgtttcggc agttcaagct gcaggatgcg atagcggccg
     1141 atctgatcgt ccagcttttg catctccacg agggtcttga gttgttcgat aatcatactt
     1201 actcctcgaa taagaggctg atatccagcg atttgtagga atgggtgata gacccgctgg
     1261 agatgtagtg cactcccgcg cgggcgtatt ttgctatgtt ttccggagtt actcctccgg
     1321 agatttccag ctccacctgt ttacggtatt tcttggccgc tgccttgatg gcggggatgt
     1381 ccatattgtc cagcatcacg cgatcggctc cagccgctac ggcctcggca agttcttccg
     1441 gattggtgac ttccacttcc accttatagc tggtgttgtg ggatttgacc ctgcgcatgg
     1501 cttccgtgat ccctccgcag gcgcggatgt ggttttcctt gagcatcacc atgtcataga
     1561 ggccaaaacg gtggttgaac ccgcctccaa ccctcacggc atacttttcc agcagtctca
     1621 gcatgggagt ggtcttacgg gtatcgagca accggactcc cgtgccttcc agggcattca
     1681 ccatctggcg ggtttgcgtg gcaatgccgc tcagcctctg taaaaagttg agcgctaccc
     1741 gttcacccat cagaatcgag gcggctttgc cctcgatgct gagaatctcg tccttgggct
     1801 gcacctgatc tccatccttg cgatagacgg tgattttgag atcggggtcc acagtcttga
     1861 acacctgttt ggccacctcg acgccagcca ggacaccagg agctttggca accataaacg
     1921 ccctgctgct aacgggatcg aggtccagat aggcggcagt gatatcaccg ctgccgaggt
     1981 cttcgcttaa ggcttccctt actatgtcat tgatttgcat gctgcctccc caagacaaaa
     2041 aaaaggcatc cgcaaatatg caaatgccta acgccgttaa ggctggtgtt cttgtctata
     2101 ttcttacttc gtatacacat atgtaataaa acctcgatgt atgagccact aagaaaaaaa
     2161 agccaaatct gtcaagcaca atgcgtgact gtggttttta aaacagaggg actgagagac
     2221 agagactttt gagggacaga gaattgagga ttgaaagcgc tcattcctgg agccgcaaac
     2281 gtcgtcgatt gcgtgtgggc agacgtttca aacaaacctc agagaaagcg gagaaagcag
     2341 agaaaacaga gggtttattt acaatgagaa atgagatggg gaaacaatga gacatgagaa
     2401 tatgaggggc cttgacaatg agaatatgag attggaaaac aatgagatat gagaatatga
     2461 gggaccttga caatgagaaa tgagaatttg agccaggaaa acaatgagac atgagaatat
     2521 gagggctgga taaaatccaa cccttaattg cagtaagtca atatcgcggg aactcaccag
     2581 ctcgcagagc tctcaccagc aggaactgct ctcacc
//
LOCUS       DAOSFN010000211         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_38944_length_2572_cov_10.692094, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000211 DAOSFN010000000
VERSION     DAOSFN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38944_length_2572_cov_10.692094"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1886)
                     /locus_tag="PLO35_09580"
     CDS             complement(210..1886)
                     /locus_tag="PLO35_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH79959.1"
                     /translation="MKQSLLISLLLITALSLSASPWNALGDTLTVIQMPILNVPAIHI
                     PGETLQITCLAPQSTTGWQAALIHGSKNIPLQIGSSQYLSSPDRWELSATLPNIPVFE
                     LYDLRVTASGGLDDTTWNAVKLVPSRKSSYYFVHVTDSHMPTRVYYPDPGFDTDSLAV
                     NDFRAVMDDINLINPEFVLFTGDLINEGELENFAGQHWYGWIQRILNQFDVPVYVTNG
                     NHDIGGWNSTPAPQGSSRRSWWRYFGWPWLDSTNAAWGRFTQDYSFVYGDVHYIGLEA
                     YDNYDNWRAQIYGGQSFILSQMQWLNAQMQLYPDKTRVLFYHYDFSDQLNLSTLGADM
                     GLWGHIHSNNGSTSTYPYNLATRSTCDGNRAYRVVRVNGTQLQPLSTIYAGYSGSNIS
                     ATFQPSNLAVADSVRAIFVNTQSQSFEHSLLRFNMPPGPYDYVVYNGSLTQVDRSPQR
                     NVVYVNVNLSANATQYVSVASRPSSSTDDPTAPTPPLIHNIYPNPFRSALTIELSGAG
                     KSPSSLYIYDLKGRRVDCIEVPDRAEVINWDSGDLPSGIYFLGGKKIVKL"
     gene            complement(1984..2433)
                     /locus_tag="PLO35_09585"
     CDS             complement(1984..2433)
                     /locus_tag="PLO35_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOH79960.1"
                     /translation="MENDERMKQLTHNIIGACIEVHRILGPGLREKMYRDCLCIELEL
                     RGLAFEKEKTVFYDYKGHKMEADMRADIIVENSVVLELKSVVQMHPVFEAQLLSYLKV
                     TNIPVGLLINFNVKLLKDGVIRRTLDGLGKPSPNHSFNTQAHAHEHI"
BASE COUNT          566 a          612 c          746 g          648 t
ORIGIN      
        1 gcgttatgcg ctcacgcgct gaattggaat tcgatgctac atacccacgg aaacgagacg
       61 tttccggctc caccagtgtg cgctccagtc aggttttttc ccactattca attctcaatt
      121 gtcaattctc aattctatgt ccctccgtcc ctctgtttta acaaacctct ctgcttttct
      181 ctgtgtttaa gtagtgtttt atcagtgttt cacagcttaa ctatcttctt gccgcccagg
      241 aagtagatcc cgctgggcag gtctcccgaa tcccagttga tcacctcagc gcggtccggc
      301 acctcgatac aatccacccg gcggcctttg aggtcataga tatacaggct ggagggggat
      361 ttcccggccc cggagagttc gatggtgagg gcagagcgga aaggattggg atagatgttg
      421 tgaatcaggg gaggagttgg agcggtggga tcgtcggtgg aggaagaagg acgcgaagcc
      481 acgctcacgt attgggtggc attggcgctg agattcacat ttacatacac cacgttgcgc
      541 tggggtgagc ggtccacctg agtgaggctg ccgttgtaga ccacatagtc atagggcccg
      601 gggggcatat tgaagcgcag caggctgtgt tcaaagctct gggactgggt attcacaaag
      661 atcgctctca cgctgtccgc gacggccagg ttggaaggct ggaaggtggc gctgatgttg
      721 ctgccgctgt agccggcata gatggtgctg agaggctgaa gctgagtgcc gttgacgcgg
      781 accactctgt aggcccggtt gccgtcacag gtgctgcggg tggccaggtt gtagggataa
      841 gtgctggtgg agccattgtt ggagtggatg tggccccaga ggcccatgtc cgctcccagg
      901 gtgctgaggt tgagctgatc ggagaaatcg tagtgataaa agagcactct ggtcttatcc
      961 ggatagagct gcatctgggc attcagccac tgcatctgag agaggataaa gctttggccg
     1021 ccatagatct gagcgcgcca gttgtcataa ttgtcatagg cttccagacc gatgtaatgg
     1081 acgtcgccat acacgaagct gtagtcctgg gtaaatctgc cccaggcggc gttggtgctg
     1141 tccagccagg gccagccaaa atagcgccac caactgcgtc tggagcttcc ctgcggggcg
     1201 ggagtgctgt tccagccgcc gatgtcgtga ttgccgttgg taacgtaaac cggcacatca
     1261 aactggttca gaatcctctg aatccagcca taccaatgct gtccggcaaa gttttccagc
     1321 tcgccctcgt tgatcaggtc tccggtgaag agcacgaact ccggattgat gaggttgatg
     1381 tcgtccatca cggcacggaa gtcgttgact gccagagagt ccgtgtcgaa accgggatcg
     1441 ggataataga cgcgggtggg catgtgggaa tcggtgacgt ggacgaaata gtagctggat
     1501 ttacgggagg ggaccagctt gacggcgttc caggtggtgt cgtccaggcc tccggaagcg
     1561 gtgacgcgga ggtcgtaaag ctcgaagacc gggatgtttg gcagggtggc gctgagctcc
     1621 catctgtccg gagagctgag atactgggag gagccgatct gcagagggat atttttgctg
     1681 ccgtgaatca gagcggcctg ccagcccgtg gtgctctgag gagccaggca ggtgatctgc
     1741 agggtctcac ccggaatgtg gatggccggg acgttgagaa tgggcatctg gatcacggtg
     1801 agggtgtcgc cgagggcgtt ccagggactg gctgaaaggg acagagctgt gatgaggagc
     1861 agagatatca agagagattg tttcatgtgg ctacctttct tttttaaaac agagggacag
     1921 agggacagag atttgagaga aacagaggat tttttgtaat gaaggtggga gtaatatggt
     1981 gtgttagata tgttcgtgcg catgagcctg tgtattaaat gaatgattgg gggatggttt
     2041 tcccaggccg tccagggttc tacggataac gccatctttc aagagcttga cattgaagtt
     2101 gataagcaga cctactggaa tgttagtgac tttcaggtag gaaagcagtt gtgcttcaaa
     2161 aacgggatgc atttgaacca ccgacttcag ctccagaacc acagagtttt ctacaatgat
     2221 atcagccctc atgtcagcct ccattttgtg gcctttataa tcgtaaaaca cggtcttttc
     2281 tttttcaaac gccagtcctc tcaactccaa ttctatacac aaacaatctc gatacatctt
     2341 ttccctcagg cctggcccca ggattcgatg tacttctatg caagcaccga tgatgttgtg
     2401 cgtcagttgt ttcattcttt catcattttc catagttaat cccctgcttt atacgatact
     2461 cgttcgcggc atgtcttgaa ccataatttg tccccgcatt ctaatccatt tatctcacaa
     2521 ttcattatat tcaattctct gtctctcaag aatctctgtc cctctgtccc tc
//
LOCUS       DAOSFN010000212         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_42585_length_2421_cov_7.751057, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000212 DAOSFN010000000
VERSION     DAOSFN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42585_length_2421_cov_7.751057"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1101)
                     /locus_tag="PLO35_09590"
     CDS             complement(10..1101)
                     /locus_tag="PLO35_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79961.1"
                     /translation="MNPRVPTLLTILIFCLTLFGQTSFTATYTFGSDGNVSSFAYNGS
                     SYTGLSFSTIEKVGVTSSSSSGNFRATGWPTGATSGSDSFTGSVDLGKYIGFSLSASE
                     GYTLSVSSITFGIGRSATGTRQAQWRGSADTYGTVLSSYNNINSGLTNSSGVLTNPDS
                     DSNWTGNILSPGSSYSNVSNAGFRLYLFNAEAGTGTAGLQGPITISGTVSSAILPAPS
                     SQASGISFANVSQNGMDISWTSGNGAQRLVLARAGAAVSSNPSDGNDYSASSVFGSGD
                     QIGSGNYVVYEGSGTSFSLSGLTASTTYNIRVYEFNNSGANTRYNINSATSNPASQAT
                     PAPLPVPGPPAATAASQVEQRFGGHSIPF"
     gene            complement(1321..2100)
                     /locus_tag="PLO35_09595"
     CDS             complement(1321..2100)
                     /locus_tag="PLO35_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79962.1"
                     /translation="MKAIWRLALIAMLILVLGLVACGKDDDDDDNNDLTGPGDITPAN
                     TDWEITIIHNPGAQKLETYYVMVDWLGDISALAETDTYSMKIDNVEYPLSSYWMFGIV
                     MITGEVELNPGHTYNLEIYRNGNKICDANIKMPYPASATFPANYSPSSAASFSWTLSD
                     NNQYQFAGVSSYGGTDQNEDAIVQVASSARSHTVAANTISGFGPGTEYELLVSQLNFA
                     RDGRTAISAVQGDYKSYGGAKGDHVQNLLRQTRRFIRSQAL"
BASE COUNT          566 a          582 c          667 g          606 t
ORIGIN      
        1 taaagcagat caaaacggta tgctgtggcc cccgaaacgc tgttccacct ggctggcagc
       61 cgttgctgca ggtggacccg gaaccggaag aggggctgga gtagcctggc tggctgggtt
      121 tgaggtggct gaattaatgt tatatctagt gttggcaccg ctgttgttga actcgtaaac
      181 cctgatgtta taggtggtgg aagcagtcaa accgctgagg ctgaagctgg tgccgctgcc
      241 ttcatagacc acatagttac cgctgccgat ttggtcacca gagccaaaaa ctgatgacgc
      301 gctgtaatca tttccatcac ttggattgct gctgaccgcg gctccggctc tggctaaaac
      361 cagcctttga gctccatttc cgctggtcca ggatatgtcc attccgttct ggctcacgtt
      421 ggcaaagctg atcccgctgg cttgtgagga tggtgctggc agaatggccg aactcactgt
      481 cccggatata gtaatggggc cctgcagtcc ggcagtgcca gtgccagctt cagcattgaa
      541 aaggtataga cggaatcccg cgttgctgac gttgctatag ctgctgccgg ggctgaggat
      601 attgccggtc cagtttgagt cggaatctgg attggtcaag accccggagc tgtttgtcaa
      661 tcctgagttg atgttattgt aggagctcaa cacggtccca taagtatcag cgcttccacg
      721 ccattgggct tgtcgggtcc cggtggcact tctaccaatt ccaaaggtga tagaacttac
      781 agacagagtg taaccctctg acgctgagag agaaaagcca atatacttcc caagatcgac
      841 actgcccgtg aagctatcag atccactcgt cgctccagtc ggccaacccg tagccctgaa
      901 attccctgag ctggaggaac tcgtcacccc cactttctca atggttgaaa agctcaggcc
      961 tgtatagctg ctgccgttgt aggcaaagct ggagacgttg ccgtctgaac caaaagtata
     1021 ggtggcggtg aaactggtct ggccaaagag ggtcaggcaa aaaatcagta tcgttagaag
     1081 cgtcgggacg cgtggattca tgttatcctc gggattgtcg tattggtatg gaaagcggtc
     1141 tatacagacg caaaagccgg tctgctatat aaatccgctt ccagaatgcc ccatgtttca
     1201 gacaggccgg atttgtccat agttaatcca gggcctccat caggggcgtg gaatcttgtc
     1261 cacaaaaacc aggccgcggc tgatagaccg cggccagaaa tggggctgtg atttagaacg
     1321 ttacagggct tgcgagcgga taaaacgacg cgtctgcctc aggaggtttt gaacgtgatc
     1381 accttttgcg ccaccatagc tcttgtaatc accctgaacg gcggaaatgg cagtgcggcc
     1441 atcgcgggca aaattgagct gggagaccag gagttcatac tcggtgccag gaccaaaacc
     1501 gctgattgtg ttggccgcaa ccgtgtggga acgtgccgac gaggccacct ggacgatggc
     1561 gtcttcattc tgatcggtac cgccgtagga agaaacgccg gcaaattgat actggttgtt
     1621 gtcagataag gtccaactga agctggcagc gctgcttgga ctgtaattgg ccgggaaagt
     1681 agctgaagcg ggataaggca ttttgatatt ggcatcgcag atcttattgc cgtttctgta
     1741 gatttccagg ttgtaggtgt ggccaggatt gagctccact tctccggtga tcatcacaat
     1801 gccaaacatc cagtagctgc tgagaggata ttccacattg tcgatcttca tagagtaggt
     1861 gtccgtctcc gccaaggcag agatgtcgcc aagccagtcc accatcacat aatatgtttc
     1921 cagcttctgg gctcctgggt tgtggataat ggtgatctcc caatcggtgt ttgccggagt
     1981 gatatcaccg gggccggtga gatcgttgtt gtcgtcatcg tcgtcatctt tgccgcaggc
     2041 aacgaggccg aggaccagga tcaacatggc aatcaaggct aatctccaga ttgctttcat
     2101 gataaactcc ttatgttttt attctgccgt caagtatgag gaggctgtga aaactgtcaa
     2161 ctgaaaactg ctaccggagt cttttattgg gaaaaattga gttgacggat tgggggccat
     2221 ccggaaaatg tattacgctg ggtttttacc tccgtgtgaa acttggcaag ccctttatct
     2281 acgtttatat tgtatttagg caggcctatc tggccgccat tttacgctct ggtttcaata
     2341 tctatatcgg tgtgccggct tacaggcacg ccaaatcgta attttttaga cttaagcaag
     2401 gagatgaaca tgcttagaaa a
//
LOCUS       DAOSFN010000213         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_42784_length_2414_cov_10.315388, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000213 DAOSFN010000000
VERSION     DAOSFN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42784_length_2414_cov_10.315388"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..337)
                     /locus_tag="PLO35_09600"
     CDS             complement(170..337)
                     /locus_tag="PLO35_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79963.1"
                     /translation="MNNEVMEMLAKELATRVNKAVNLPLINEENEQAFFELIILMVLD
                     LVFARMEKEGW"
     gene            complement(351..806)
                     /locus_tag="PLO35_09605"
     CDS             complement(351..806)
                     /locus_tag="PLO35_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79964.1"
                     /translation="MKVKFKYGIRTYSGTIDEMTYGNYKKGQVCISRKWVIPKLTENN
                     STMGAIAKNLSTLWQQADAGYKADFKTYAGRYGAQVSAPDKLPPTGYSLWVKAMYAWA
                     KDEDPDLDLSSLTFEDVETLGSKVASVSACVNNNYLPNVNPVDDLDATI"
     gene            1265..2350
                     /locus_tag="PLO35_09610"
     CDS             1265..2350
                     /locus_tag="PLO35_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH79965.1"
                     /translation="MRFLHRDLFDSHILKEFSRALKTAAFMPSRLPFIVRALRDQDRA
                     AKLRAQLAKGGLEVPPLLIISVTKRCNFNCSGCYSKILHQDDGDEMGPERFDELLREA
                     TELGISIVMLAGGEPLTRRELLEIAARFQHITFPIFSNGSLIDEEYAAFFGRHPNLIP
                     VISLEGGQHETDLRRGYGSFDAFRDAAWVLNRNHVYWGVSITLNTDNYPLVLSKHFCR
                     DINSLGSRLFFYVEYVPIAADSQALVLKEEQKRELAERIDQLRRDVSAIFIAFPGDED
                     QYGGCLAAGRGFLHINPSGRVEPCPFAPYSDSDLRCSSLREVLSSPLLAKIRAQHQLL
                     VEGLGGCALWANRELVDQMLSEDSKAR"
BASE COUNT          521 a          669 c          633 g          591 t
ORIGIN      
        1 cctctgcttt tctctgtgtt gaaaaagtta agcgttacgc aaaaaaaggg gaacgcaagc
       61 tcatcgcctc cgttcccctt tcggcattcc tcagatcatc tgcccagcag gtgtctgatc
      121 gcctgtttcg ctttttgggc cgctttccgg accagcttca gatagtggct caccagccct
      181 ccttttccat gcgcgcgaag accaggtcca gcaccatcag gataatcagt tcaaagaagg
      241 cctgctcgtt ctcttcgttg atcagcggca ggtttaccgc cttgttcact cgggtggcca
      301 gctctttggc cagcatttcc atcacttcgt tattcattct gtccccccgt tcagatcgtg
      361 gcgtccaggt catcgaccgg attcacgttg ggcaggtagt tgttattgac gcaggcgctt
      421 acggaagcca ccttgctgcc cagggtctcc acatcctcaa aagtgaggct gctgaggtcc
      481 agatcggggt cctcatcctt ggcccaggca tacatcgctt tcacccagag gctgtaaccc
      541 gtgggcggca gcttgtcggg agcggagact tgagcgccat agcgtcccgc gtaggtcttg
      601 aaatcggctt tgtagcccgc atcggcctgt tgccacaggg tggagagatt cttggcgatc
      661 gcacccatgg tgctgttgtt ctcggtcagc ttggggatca cccacttgcg gctgatgcac
      721 acctggccct tcttgtagtt gccataggtc atctcatcga tcgttccgga ataggtccgg
      781 atgccatact tgaatttgac tttcatgata tcctcctcag gattggtttt attgaggcat
      841 cagcatcatc ggctggttca atatcagcgt caccaaaccc agaacgcggc cgtcgcacca
      901 gtgcttcagt taagctgatt atcagataag ccaatttgta gatgtgcctc tataatacta
      961 agggcgacat cgtcgatgcg gagattttac tcgtctggat tccagcctat cttcatggct
     1021 cctattacga tacaaaaaac atataaatat accgggatca ctgggaaata gtttttattg
     1081 cgcttttatg tgccggggtc gtggcgtctg tcattaccgt ggagatgaaa accaaccctg
     1141 agtgctgcat ttcacgcgtt aagagcagcg cgtaacgcta agcgcaaaaa aaacttaaaa
     1201 cttctttctt gacacccgga cgctgctcaa atatctggtc gccaaagcga ggtatgaaaa
     1261 caatatgcga ttcttgcaca gagacttatt tgattctcat attctaaagg agttttcccg
     1321 ggccttgaaa accgcggcct ttatgccctc ccggctgccc tttattgtca gagcgttgcg
     1381 ggatcaggac agggctgcca aactgagggc ccagctcgcc aaagggggtt tggaagtgcc
     1441 tccgctgctt atcatcagcg tcaccaaacg ctgcaatttc aactgctcgg gctgttattc
     1501 caaaatcctg catcaggacg atggcgatga gatgggtccg gagcgttttg acgagctgct
     1561 gcgcgaggct accgagctgg gaatctccat cgtgatgctg gctggaggcg aaccgctcac
     1621 ccgcagggaa ttgctggaaa ttgccgctcg ctttcagcat atcacctttc ctatcttcag
     1681 caacggctct ttgatcgatg aggagtatgc cgctttcttt ggtcgccatc ccaacctcat
     1741 ccccgtgatc agtctggaag gtggccagca cgagaccgat ctgcgccgcg gttatggcag
     1801 ctttgacgct ttccgggacg cggcctgggt gctgaaccgc aatcacgttt actggggtgt
     1861 ctccatcact ctcaacaccg ataactaccc gctggtattg tccaaacact tctgccgcga
     1921 catcaacagc ctcggcagcc ggctgttttt ctatgtggaa tacgtgccca tcgcggcaga
     1981 cagccaggcc ctggtgctca aggaagagca gaaacgggag cttgctgagc ggatcgacca
     2041 gcttagacgg gatgtctcgg ctattttcat cgctttcccc ggagacgaag atcaatatgg
     2101 aggctgcctg gccgccggaa ggggattttt gcacatcaac cccagcggca gagtggagcc
     2161 atgtcccttc gcgccttatt ccgactcgga ccttcgctgc tccagcctca gagaggtgct
     2221 gtcctcacct ctgctggcta aaatccgcgc ccagcaccag ctcctggtcg aaggactggg
     2281 gggctgcgcg ctctgggcta accgcgaact ggtggaccag atgctcagcg aggacagcaa
     2341 ggccagataa cacttcacta cagagggact gagaattgag agtgaataga acgcagaaaa
     2401 aaggaaaaag ggat
//
LOCUS       DAOSFN010000214         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_44236_length_2361_cov_9.070252, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000214 DAOSFN010000000
VERSION     DAOSFN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44236_length_2361_cov_9.070252"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="PLO35_09615"
     CDS             complement(<1..1974)
                     /locus_tag="PLO35_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH79966.1"
                     /translation="MRLKALSYCLLLAMIQGLPLGAQAPASGISGPFMYFTDAAQAHP
                     APRGQRFPNSGYFTIGAGGEFSNFTQAINHINSLEIIGIGGFHFEVLAGSVFNENPPA
                     ITKVASEASPVIFQKVGTGPNPIIRATGTTESGEAIIHLNGVSYYSFDGIDLANYGTT
                     TAMEYGYYLSTSQGRACGYNTISNCQITLSSANANSKGIYAASGTGSRNYYNNYQNIT
                     INGANYGIHLLGSSTQYHLGELISGCVITNAGRCGILSPYGEGTVIENNQISVRSGIT
                     QSFEGISFGGSSATALVNGNQIIGTSVTRAVTGIYQSSGTATITNNTIRNFSSNDNNF
                     TGILIAAGDVTIGTNTIRDNSSNQNICGIQVQSEVSSATVTGNRVHTMSNNPSSSGSY
                     YCAGIEAGGANTLLANNMIHSLSAAATNAPALRGISVLAGSSLRIIFNTVSLSASAPH
                     SGFSSAALYIAAANNSIELINNIFANQSTPGAGTGGRSVAMWKDVAGFAELSLSCNRN
                     LYWAGTPDAKRLIVYAAGAGYQNLIGYQLVSGGRDANSWQEAPPFVAWNDPHIQSGIQ
                     TNVESNALPIIGIELDFDGDTRSATHPDIGADEGFFGETGWTCAPEQLDLGFVCLGGE
                     PGQASLTLSNLGSGILSFNSSGFVISGIDAASFA"
BASE COUNT          462 a          599 c          750 g          550 t
ORIGIN      
        1 ggcaaagctg gcggcatcga tcccggaaat cacaaagccg ctggagttga agctcaggat
       61 gccgcttccc aggttggaga gcgtgaggct ggcctggcct ggctcgccac ccaggcagac
      121 gaagccgagg tccagctgct ccggagcgca ggtccagccc gtctcgccaa agaatccttc
      181 atcggcacca atatcgggat gggtagcgga gcgcgtgtcg ccgtcgaaat ccagctctat
      241 ccctatgatg ggcaaggcgt tggactccac gttggtctgg atgccgctct gaatgtgggg
      301 atcgttccag gccacaaaag gcggggcttc ctgccaggaa ttggcatccc ggcctccgct
      361 caccagttga taaccgatca ggttctggta gcctgctcct gcggcataga cgatcagccg
      421 cttggcatca ggtgttccgg cccagtagag gttgcggttg cagctgaggc tgagctcggc
      481 aaagcccgcc acgtctttcc acatcgccac actcctgcct ccggtcccgg cgccgggagt
      541 gctctgattg gcgaagatat tgttgatcaa ttcgatagag ttgttggccg cggcgatgta
      601 taaggccgcg gaactgaaac cgctgtgggg agcggaagcg ctgagcgaca cggtgttgaa
      661 gatgatcctg aggctggagc cggccaaaac actgatcccc cgcagagctg gagcgttggt
      721 ggcagcggcg ctgaggctat ggatcatgtt attggccagc agcgtgttgg cccctcccgc
      781 ttcgattccg gcgcaataat atgagccgga ggaggagggg ttgtttgaca ttgtatggac
      841 gcggttgccg gtcacggtgg cagagctgac ctcgctctgg acctggatgc cgcagatgtt
      901 ttggttggag ctgttgtccc ggatggtgtt ggtgccgatg gtcacgtctc cggcggcgat
      961 caatatgcct gtgaagttgt tgtcgttgct gctgaaattg cggatcgtgt tgtttgtaat
     1021 tgtcgcggtg ccagagcttt ggtagatccc ggtgacggcg cgggttacgg aggtgccgat
     1081 gatctggttg ccgttcacca gcgcggtggc gctactccct ccaaagctga tcccctcaaa
     1141 ggattgcgtg attccggagc ggacgctgat ctggttgttt tcgatcacgg tgccctcacc
     1201 gtagggactc aagatgccac agcggccggc attggtgatc acgcagccgc tgatcaactc
     1261 tccgaggtgg tattgagtgc tgctgcccag caggtgaatg ccgtaattgg ccccgttgat
     1321 ggtgatattt tgatagttgt tgtaatagtt tctggagccg gtaccgctgg cggcatagat
     1381 gcctttgctg ttggcattgg cggagctgag cgtgatctgg cagttgctga tcgtgttgta
     1441 tccgcaagcc cggccctggg atgtgctgag gtaatagccg tattccattg cggttgtggt
     1501 gccgtaattg gccagatcga tgccgtcgaa gctgtaataa ctgacgccgt tgagatggat
     1561 gatggcctct ccgctttcag tggtcccggt ggcgcggatg atggggttgg gtcccgtccc
     1621 aaccttctga aagatcacgg gcgaggcttc cgaggccact ttggtgatgg cggggggatt
     1681 ttcattgaag accgagccgg ccagcacctc aaagtgaaat cctcctatcc ctatgatctc
     1741 caggctgttg atgtgattga tagcctgggt gaaattgctg aactcaccac cggccccgat
     1801 cgtgaaatag ccgctgttcg ggaaacgctg tccccggggg gccggatggg cctgagccgc
     1861 gtccgtgaaa tacataaaag ggccggatat tccagacgct ggggcttgcg ctcccaaggg
     1921 cagaccctgg atcatggcca gcaacagaca gtatgacaac gctttaagcc tcataggtat
     1981 ctcctttgtg gagggagtat tggtatgttg aagcgacact ttgtaaacat tttcaggatc
     2041 gctgagggga gaaaatcagt caaggaaaat atgcggcact cgcgggcact ggactggata
     2101 tgcatgtggg ataacgcgtt acatgttgtg taacactggc tttagccggt cgtagtgccg
     2161 gtcttcagcc ggcaccatcc tggccccgtt ggagtatata gaaaaaaagc ggcatatcat
     2221 attgggagac atcccggtgg tttcgcaccg gctgaagagc ttgtgcgaaa atggccgtct
     2281 tggagttcaa agcccctaaa tagattatgc gtttatctct atacctgacg gggctttgga
     2341 cttacgtgca gcacgtcgaa g
//
LOCUS       DAOSFN010000215         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_45133_length_2331_cov_10.178822, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000215 DAOSFN010000000
VERSION     DAOSFN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45133_length_2331_cov_10.178822"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..633)
                     /locus_tag="PLO35_09620"
     CDS             complement(100..633)
                     /locus_tag="PLO35_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH79967.1"
                     /translation="MKKLITLTMLAFASLALFAVIDLDTPPTHYSGSIIIKNDRPWLK
                     VTEPSLELRLLLAPQAELDSLKLSFAEAEEVTIEGIREGDLLLVSKVQKGEILWIIRD
                     FSIGAVYPEGATYKVDSKKCIACKLCLKPCPTGAISMVKGKAVIDLSKCTECGICIEG
                     HYPFRGCPTKAISPQTP"
     gene            complement(630..1685)
                     /locus_tag="PLO35_09625"
     CDS             complement(630..1685)
                     /locus_tag="PLO35_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOH79968.1"
                     /translation="MLAVIMAGGAGTRFWPASRAALPKQFLPILGSKSMIRLTMERLL
                     PLVPVDKIHVVTAASQVELVKQHLPEMPVENIIIEPFGMNTAPCLALSLAVLLDRHSL
                     EETMLVLPADHFIRDTQSFLASLTKADEVASKGRLVTFGIIPDHPATGYGYIEAGAEL
                     SPGVREVARFKEKPDALTAAQFIAQGNFYWNSGMFCWSLGTLARAFETHLPLALNVAR
                     ELVAGLIRGIDEATAASVYARMPKTPIDIGIMEKATNRAVIPVNYGWSDVGGWKALAD
                     LSPTDANGNRFSAPSLPLDSTDNYISTNKFCALIGVHGLCLIETPDAILLCPKDRSEE
                     VKQVVDTLKAQAKDNLL"
     gene            complement(1695..2312)
                     /locus_tag="PLO35_09630"
     CDS             complement(1695..2312)
                     /locus_tag="PLO35_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HOH79969.1"
                     /translation="MKKLIPLLILTLLSLSLSAISSADLYKKLQSAYKGLSSYQANVS
                     QSNYYPQLKKTITYTGKIYFTPGKMLMHFEKPNLQRLHIEAGQVTLYDALSNTIFTSA
                     IQPQYGRMNPLEILQLYWDRSSVKLGKQKGDLVDVTLTPAKDAMLSSLSATINSKNGQ
                     VQKLSYKDAGGNTVSYSFSGIKLNGGISASVWKFSYPKDAQRIDQ"
BASE COUNT          440 a          551 c          774 g          566 t
ORIGIN      
        1 cctctccgta tctctctctg tgtcctctgt agctctgtag tgaaaaagga aagctctgta
       61 actcagtagt taaagtaatc tctggattgg aatccgacgc tacggagtct gaggcgagat
      121 ggccttggtg gggcagcccc ggaagggata gtggccttcg atgcagatgc cgcattcggt
      181 gcatttggag aggtcgatca cggctttgcc tttcaccatg ctgattgctc cggtggggca
      241 gggtttgagg cagagtttac aggcgatgca ctttttgcta tctaccttgt aggtagcgcc
      301 ttcgggatag acggccccga tgctgaaatc gcggatgatc cagaggattt cgcctttctg
      361 caccttgctc accagcagca gatcgccttc ccggatgcct tcgatggtca cttcctcggc
      421 ctcggcaaag ctgagtttga ggctgtccag ttctgcctgg ggggccagca agagacgcag
      481 ttccaggctg ggttcggtca ctttcagcca ggggcggtcg tttttgatga tgatgctgcc
      541 gctgtagtgg gtgggcgggg tgtccagatc gatgacggcg aagagggcca ggctggcaaa
      601 ggccagcata gtgagggtaa ttagcttttt cataagagat tatccttggc ttgcgctttg
      661 agtgtgtcca ccacctgttt cacttcctcg ctgcgatctt tggggcagag gaggatggcg
      721 tccggggttt cgatcaggca gagtccatgc actccgatca gagcgcagaa tttattggtg
      781 ctgatgtagt tatctgtgga atctaagggc agggaggggg cggagaatct gttgccatta
      841 gcatcggtgg gagagagatc ggccagggct ttccagccgc ccacgtcgct ccagccatag
      901 tttacaggga tgacggcgcg gttggtggcc ttttccatga tgccgatgtc gatgggggtt
      961 ttgggcatgc gggcatagac tgaggccgcg gtggcttcgt cgatgcctct tatgaggccc
     1021 gctaccagtt ctctggctac gttaagtgcc aatggcagat gcgtttcaaa ggcgcgggca
     1081 agcgtgccga gactccagca gaacatgccg gaattccagt agaaattgcc ctgggcgatg
     1141 aattgagccg cggtgagggc gtcgggcttt tccttgaagc gcgccacctc tctcacacca
     1201 gggctaagtt ccgctcccgc ttcgatatag ccatagccgg tggcagggtg gtccgggatg
     1261 atgccaaagg tgacgaggcg tcctttgctg gccacttcat ctgctttagt gaggctggcc
     1321 agaaagctct gggtgtccct gataaagtgg tcggcgggga ggacgagcat agtctcttcc
     1381 agagagtgac ggtccagcag gacggccagg ctgagagcca gacagggggc cgtgttcatg
     1441 ccaaagggct cgataatgat gttctccacc ggcatttctg gcaaatgctg tttgaccagt
     1501 tccacctggg aggcggctgt gaccacgtgg attttatcca ccgggaccag aggcaggagg
     1561 cgttccatcg tgaggcggat catggatttg gagcccagaa tgggcaaaaa ctgcttgggc
     1621 agggcggctc tgctggcagg ccagaaacgg gtgccggccc ctccggccat tatcactgca
     1681 agcatgctaa actcctattg atcgatgcgt tgggcgtctt tgggatagct gaatttccag
     1741 accgaggcgg aaatcccgcc attgagcttg atgccggaaa agctgtagct gacggtattg
     1801 ccgccggcgt ctttgtagct gagcttctgc acctggccgt ttttggagtt gatcgtggcg
     1861 ctcagggaac tcagcatggc gtctttggca ggggtgaggg tcacatccac cagatcgcct
     1921 ttctgcttgc cgagtttgac ggaagagcgg tcccaataga gctggaggat ttccagggga
     1981 ttcatgcggc cgtattgagg ctggatggca gacgtgaaga tggtgtttga aagggcgtcg
     2041 tagagggtga cctggccggc ctcgatatgg aggcgctgga ggttgggctt ctcgaagtgc
     2101 atcagcatct taccgggagt gaaatagatc ttgccggtgt aggtgatggt ctttttgagc
     2161 tggggatagt agttgctctg ggacacgttg gcctggtagc tgctgaggcc tttgtaggcg
     2221 ctctggagct tcttgtagag gtcggcgctg ctgatggcgc tgagggaaag gctcaggagc
     2281 gtcaggatga ggagggggat gagttttttc atttgcgtta tccgttatgc g
//
LOCUS       DAOSFN010000216         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_51190_length_2139_cov_9.442418, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000216 DAOSFN010000000
VERSION     DAOSFN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51190_length_2139_cov_9.442418"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /locus_tag="PLO35_09635"
     CDS             complement(<1..2036)
                     /locus_tag="PLO35_09635"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOH79970.1"
                     /translation="MIPSYEELQRLSDSAKPECEAMLAALKQIVIPTALPMREFFDQA
                     RAGMLQAAKINSARLADPLFKNTPIQTLQQLYADYYAILSPEGGYQNCAGDPDFANRS
                     FGAQFAGLLSAFYIETRSILRYLFTGDYYSALATLKLYNKWLQRAKDGQLEYSDWLGD
                     YRDFSLQDIRIEVARRMNSRFNPQADHYRALVESADLENLSYLYLYGVYIADHDLAMA
                     RFMASYDQKELKKLAAFIVKSWTDGFERAKRDFRKKRHPLLMFPCGMERLARYIISEL
                     RALGMEPLLMLPASDGINRQVGYDHRFDEALILDRALVDLNLEVQEEAMASLQELISL
                     QAGPVYVDLFGEPPFVPVAKDTAVKFSPEQQQLGRELSAKSGQIMYKYYRRDETSFTI
                     IAFPSPRIGDQFEDIFRDTLRINLLDSDRYARIQQHIIDVLDTAEYVHVKGKDGNDTD
                     IKVSMHKLSDPSAQTLFENCVADVNIPVGEVFTSPKLEGTNGMLHVEDIYLGSLRYFN
                     LRISFEDGWVKDYSCSNFPDPEQAKKYVHENLIFPHDSLPIGEFAIGTNTLAYQIAHK
                     YDIQALLPILIIEKMGPHFAIGDTCFSHEEDSPHPSFVNGKEMIAVENEKSSTRKTDP
                     VNAYTNKHMDITLPYDMLASITAVAADGSKTDIIRDGRFVVPGTQELNLPLDEML"
BASE COUNT          467 a          491 c          651 g          530 t
ORIGIN      
        1 agcatctcat ccagcggcaa attgagctct tgggtgccgg gaaccacaaa acggccgtcg
       61 cggatgatat ctgttttact gccatcagcg gccacggcag tgatggaagc cagcatatca
      121 tagggcagag tgatgtccat gtgcttgttg gtgtaggcat ttacgggatc tgtcttgcgg
      181 gtggaggatt tctcgttttc caccgcgatc atctctttgc cgttcacaaa gctggggtgg
      241 ggggaatcct cttcgtggct gaaacaggtg tcgccaatgg cgaagtgagg tcccatcttt
      301 tcgatgatga ggatgggcag cagtgcttgt atatcatatt tatgggcgat ttgataggcc
      361 agggtgttgg taccgatggc aaattcgccg ataggaaggc tgtcgtgagg gaagatcagg
      421 ttttcgtgca cgtatttctt tgcctgttcg ggatccggga agtttgagca ggaataatct
      481 ttcacccagc cgtcctcaaa gctgatcctg aggttgaaat atcgtagcga gcccaggtag
      541 atgtcttcca cgtgcagcat cccgttggta ccttccagtt tgggagaggt aaacacttca
      601 cccacaggga tattgacatc cgccacgcag ttttcaaaca gggtctgggc agaggggtct
      661 gagagcttgt gcatgctgac tttgatatcg gtatcgttgc cgtctttgcc tttgacgtgg
      721 acgtattcgg cggtatccag aacgtcgata atgtgttgct ggatgcgggc atagcgatcg
      781 ctgtccagca ggttgatgcg caaggtatcc cggaagatat cttcaaactg gtcaccgatg
      841 cggggtgagg ggaaagcgat gatggtgaag ctggtttcgt cgcgccggta gtatttatac
      901 atgatctggc cacttttggc gctgagttcg cgtcccagtt gttgctgttc ggggctgaat
      961 ttgacggccg tgtctttggc cacaggcacg aagggtggct cgccaaagag gtccacatat
     1021 accggtccgg cttgtaagga gataagctcc tgcagtgaag ccatggcttc ttcctggact
     1081 tccagattga ggtccaccag agcgcggtcg aggatcaaag cttcatcgaa acggtggtcg
     1141 taacccactt gacggttgat gccatcgctg gctggtagca tgagcagcgg ctccatcccc
     1201 aaagctctga gctcgctaat gatgtatctg gccaggcgtt ccataccgca agggaacatc
     1261 agcagcggat ggcgtttctt gcggaaatcg cgtttggctc gctcaaatcc gtccgtccag
     1321 ctttttacga taaaggcggc taactttttg agctccttct ggtcgtagga agccatgaag
     1381 cgcgccatgg cgaggtcgtg atcggcgata tagactccat agagatagag gtagctgagg
     1441 ttttccagat cggcagattc caccagggcg cggtaatgat cggcctgggg gttgaagcgg
     1501 gaattcattc gtctggcaac ttcgatcctg atatcctgga ggctgaagtc acgatagtcc
     1561 cccagccagt ctgagtattc caactggccg tctttggccc tttgcagcca tttgttatag
     1621 agcttcaagg tggccagggc actgtaataa tccccggtaa agagatatct caggatggag
     1681 cgggtctcga tatagaaagc actgagcagg cctgcgaact gcgcgccaaa gctgcgattg
     1741 gcaaaatcgg gatcgcccgc gcagttttga taaccgccct ccggactcag gatagcatag
     1801 taatcggcgt aaagctgctg cagcgtctgg atgggagtgt ttttgaagag gggatcggca
     1861 aggcgcgcgg agttgatctt tgcggcctgg agcatgccgg cacgggcctg gtcaaagaat
     1921 tcccgcatgg gtaaagcggt ggggatgacg atttgtttca aagcggcgag catagcctcg
     1981 cattcaggtt tggcggagtc tgaaagcctc tgcagttcct cataggaggg gatcataaac
     2041 acctcgtaaa gttttttgtg agacaaaatt acaggagcgc cgggatgtca aggattatgg
     2101 tgagagctga agctgtgaga tacttcgtag gtgagatac
//
LOCUS       DAOSFN010000217         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_52408_length_2105_cov_8.472683, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000217 DAOSFN010000000
VERSION     DAOSFN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52408_length_2105_cov_8.472683"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1410
                     /gene="mtnA"
                     /locus_tag="PLO35_09640"
     CDS             376..1410
                     /gene="mtnA"
                     /locus_tag="PLO35_09640"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOH79971.1"
                     /translation="MKINGIDHRSVWWEDGHLLMIDQNKLPFEFAIAKYGNYMEVATA
                     IREMTVRGAPAIGAAGAYGMALAAQSAPDARFRAYMRAARDELISTRPTAIDLLNGVS
                     FVYESTIKFIPDVKHARQVAIFSAEELANTSASECYEIGKVGADLVPRGGRILTHCNA
                     GALATVDWGTALAVVRAAHHKKLDIFVYVDETRPRLQGARLTAWELEQEGIPHAIISD
                     GAAGFYFSKGEIDLVITGADRICLNGDIANKIGTYEKAVLAHEHHVPFYVAAPFSTFD
                     LSCAKGTDIPIEIRDEDEIKRFNGFMLANPGSPALNPAFDITPAKYITGIITPRGVFP
                     AKEAARKIQE"
     gene            1414..>2105
                     /locus_tag="PLO35_09645"
     CDS             1414..>2105
                     /locus_tag="PLO35_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH79972.1"
                     /translation="MRQPAIADLIVKGGTIITMDPAAPELENHSLAVKDGKIAAIYPS
                     SEDLWSAPEILDASDCLLIPGLINTHSHLPMTYFRGLADDLPLDIWLRHYIWPMEAKI
                     LNAQVIRDATLHGACEMLKNGISMSHDMYFNMPAIADACSQAGMRVLIGEAVIDAQLA
                     GDQLPGARISELRQLYQDNPLVDFDLAPHSIYACSRSTLEKCVQASEDLNVPLHIHLS
                     ETEAEVQNCLKE"
BASE COUNT          462 a          608 c          581 g          454 t
ORIGIN      
        1 atgcatagct ttatcgtggc cgcttgaact ccaacggggt aatgtcagtc tctggtttcg
       61 cgagtttttg cacaagctct taagtcagtg ttacgggttg ttacgcgaaa tgaacccacg
      121 caaacgagga cgtttgcggc tccccaagtc atcgttccct gttcaccgtt cacccttccc
      181 agttccccct tcaccgttca cagacgttgt tgtcaattct caatcctctg ttcctcaaaa
      241 aactctgtcc ctctgtccct ctgttttaaa gaacgtaccg cgtcacgcgg aaccccacag
      301 aaagctcttg acgaaatgcc tcattcccag accagggttt agatatgcat aagcacatca
      361 gttaaaggaa gataaatgaa gataaacggg atagaccacc ggagtgtgtg gtgggaagat
      421 ggtcatctgc tgatgatcga ccaaaacaag ctgcctttcg agtttgccat cgccaaatat
      481 ggcaactata tggaggtggc cactgccatc cgggaaatga cggttcgtgg agctccggcg
      541 atcggagcgg cgggggctta tgggatggcg ttagccgctc agagtgctcc cgatgccagg
      601 tttcgggcct atatgagggc cgcgcgggat gagctgatct ccacgcgtcc cacggccatc
      661 gatctgctca atggcgtcag ctttgtgtac gagagcacca tcaagttcat cccggatgta
      721 aagcatgccc gccaggtggc gatcttttca gccgaggaat tggccaacac cagtgccagc
      781 gagtgttatg agatcggcaa ggtgggcgcg gacctggtcc ccaggggggg ccgcatcctc
      841 actcattgca acgcgggagc tctggccacg gtggattggg gcaccgcgct ggccgtggtg
      901 agggccgccc atcacaagaa actggacatc tttgtgtatg tggacgagac ccgtccccgc
      961 ctgcagggag cccggctcac ggcctgggaa ctggagcagg aaggcatccc tcatgccatc
     1021 ataagcgacg gcgcggcggg attttacttt tccaaggggg agatcgatct ggtgatcacc
     1081 ggagcggaca gaatctgcct caacggcgat attgccaaca agatcggcac ctatgagaag
     1141 gccgtcctgg cccacgagca tcacgtgccc ttctatgtgg cggcgccttt ttccactttt
     1201 gacctcagtt gcgccaaagg gacggacatt cccatcgaga ttcgggacga ggatgagatc
     1261 aaacgcttca atggtttcat gttggccaat cctggttctc cggctttgaa tccggccttc
     1321 gatattactc cggccaaata catcaccggg atcatcactc cccggggcgt gtttccagcc
     1381 aaagaagccg cccgcaagat acaggagtag tctatgcgcc agccagcaat cgcggacttg
     1441 atcgtgaaag gcgggaccat tatcaccatg gaccccgcag ctccagagct ggaaaaccac
     1501 agcctggccg tcaaagacgg taagatcgcg gcaatttatc ccagcagtga agacctctgg
     1561 agcgctccgg agatcctgga cgcttcggac tgcctcttga tcccaggcct catcaacacc
     1621 cattcccatc tgcccatgac ctattttcgc ggcctggcgg acgacctgcc gctggatatc
     1681 tggctgcgtc attacatctg gcccatggag gccaaaatcc tgaacgccca ggtgatccgc
     1741 gacgccacgc tgcacggcgc ctgcgagatg ctgaaaaacg ggatctcgat gagtcacgac
     1801 atgtatttca acatgcccgc catcgcggat gcctgttctc aggcggggat gagagtgttg
     1861 atcggagagg cggtgatcga tgcccaactg gctggcgatc agcttcccgg ggcccggatc
     1921 tctgagcttc gacagctcta tcaggacaat cctctggtgg attttgacct ggcgccgcat
     1981 tccatctatg cctgttcccg ttctacttta gaaaagtgcg tacaggcctc ggaggacctg
     2041 aacgtgccgc tgcacatcca tctttccgag acggaagccg aggtgcaaaa ctgcctcaaa
     2101 gagca
//
LOCUS       DAOSFN010000218         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_53520_length_2076_cov_7.458684, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000218 DAOSFN010000000
VERSION     DAOSFN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53520_length_2076_cov_7.458684"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1492)
                     /locus_tag="PLO35_09650"
     CDS             complement(122..1492)
                     /locus_tag="PLO35_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOH79973.1"
                     /translation="MKQVSILIIIVGMLSGTLWGTKAMDPSTYKIQKVEVKGSRISSF
                     AFTSNVAYIGSDSGIKASDLNPPLVLEYYNRQNSGLPSDGVTALTVRNGKELWIGTDA
                     GLAMMTIGLIDATIPPNSPFQNAVISSLAADKDGLLWISAIREMQDEGGWIEGMGISS
                     YDGKSWKSYTPANSTLPESFIGSIHVDGQNRKWLATYYNTSERDHGSGLCSFDGKQFS
                     IHGNGKSYSSVECLAVDNAGKAWMGLAEYTEEGLLGGGLACFDGKTWTHYNTTNSDIP
                     GDLVSDITIGKDGTIWMAVLDTDPMYPYSFGLTGFNGQEWRLIPQEKLADQGRQISCL
                     ATDPDGKLWVGTDNGLAVVSFPEPIANQEHEFTPYDQPPVIVGELRPEYPDTAKRARV
                     QGTVVLEVEVYDDGRIGEIRVKRSVQGLDESAIEAVRKVKFKPGKAGGKPVNTMVIIP
                     IEFRLN"
BASE COUNT          419 a          574 c          554 g          529 t
ORIGIN      
        1 tctcattttc tcatatctca ttgttttcca gtctcattct ctcatatctc attgtaaaat
       61 aaacctctgc tttccccgct ttctccgagg tttgttggcg caaagtgaca cgcgtcacct
      121 ctcagtttaa gcggaactcg attgggatga tgaccatggt gttgacgggt ttcccaccag
      181 ctttaccggg cttgaatttc accttgcgca ccgcttcgat cgcggattcg tccagtccct
      241 gcacagagcg ttttacgcgg atttctccga ttcggccgtc gtcatagact tccacttcca
      301 ggaccacggt accctggacc cgagcccgtt tggccgtatc gggatattcg ggtcttagct
      361 ctccaacgat gacagggggc tggtcatagg gtgtaaactc atgttcttga ttggcaatgg
      421 gttccggaaa actgaccacg gccagcccgt tgtcggtgcc cacccagagc ttgccatccg
      481 gatcggtggc gaggcaggag atttgcctgc cttggtcggc cagcttttcc tgcggaatca
      541 accgccattc ttggccgtta aagcctgtca atccaaacga atagggatac atggggtccg
      601 tgtccagcac ggccatccag atggtgccgt ccttgccgat ggtgatgtca cttaccaggt
      661 caccggggat gtctgagttg gtggtgttgt agtgggtcca ggtcttgccg tcaaaacagg
      721 ccagaccgcc gcccagcaag ccttcctcag tgtattccgc caggcccatc caggctttgc
      781 cggcattatc cacagccagg cattccacgc tgctgtagct cttgccattg ccgtggatgc
      841 tgaactgctt gccgtcaaag ctgcagagcc cgctgccgtg gtcgcgctcg ctggtgttgt
      901 aataggtggc cagccatttg cggttttggc cgtccacgtg gatgctgcca atgaagcttt
      961 ccggcagagt ggagttggcg ggagtgtagc tcttccagga cttgccgtcg taactgctaa
     1021 tgcccatgcc ctcgatccag ccaccttcat cctgcatctc tctgatggcg ctgatccaca
     1081 gcaacccgtc cttatccgct gccagactgg agattacggc attctggaaa ggtgagtttg
     1141 gggggatagt ggcatcgatc aggccgatgg tcatcattgc cagaccggcg tccgtgccaa
     1201 tccacagctc tttgccgttc ctgacggtca gcgcggtcac tccatcgctg ggcaggccgc
     1261 tgttctgacg gttgtaatat tccagtacga gaggaggatt gagatcagag gctttgatac
     1321 cactgtccga gccgatatag gccacattgg acgtgaacgc gaaggagctg atccggctgc
     1381 ccttcacctc caccttctgg atcttgtaag tggaagggtc cattgccttt gtgccccaga
     1441 gcgtgcctga aagcatgccg acgataatga tgaggattga cacttgtttc atgtttggct
     1501 cccttttatc gtggagtgtt tgtcatggcg gatcgcggtt ggtttttctc ttccgttgat
     1561 tcggaaagag cgcgtaagcg gaagatatat caccgctttc caagacttta cactcgttta
     1621 tctctgttat cgtttcacgc catatcagca gacggcttct gtcaagctaa aacgccgggg
     1681 cgagtaattt ttggggcata cctgcataga tataaaacat cacaccgata caaggatgac
     1741 gtgtaaggcc gccacccctt cccgatcact atcccgtgct tatcccacgc ttatcgcacg
     1801 cttatcccgt gctttggcat gggataggca tgggatacga gtgcgagacg ggaacctggc
     1861 tggaaagcac cacctggagt tcaacgccct ggacaagcta tgcaagaccg taaataatct
     1921 gtaacactgg ctttagccgg tcgtagtgcc ggttttaacc ggtaccccgc acgccaggcc
     1981 agaagcgtta ttcaatgtaa tgaaatcgga tagaactttc gcacgcaccg actgaagagc
     2041 ttgtgcgaaa atggcgcatt gagtcccata gggacg
//
LOCUS       DAOSFN010000219         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_53891_length_2066_cov_10.590751, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000219 DAOSFN010000000
VERSION     DAOSFN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53891_length_2066_cov_10.590751"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLO35_09655"
     CDS             <1..213
                     /locus_tag="PLO35_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79974.1"
                     /translation="REINVFDVLLEVQSPVKQGGKSLFCPGALNSKAAKVVLFFTSSE
                     ASDTRLIADALSLSHLSLLKKALCFL"
     gene            complement(310..1968)
                     /locus_tag="PLO35_09660"
     CDS             complement(310..1968)
                     /locus_tag="PLO35_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79975.1"
                     /translation="MISIYIDFKLQRYQREIKYAFSFVLSTLGYSHCFISDTGQLKDN
                     DILMIYGYTDPTMEELGSIARHYITIFIQSDPDLFDPQGYTPEKLRKNLKEIKLLSPT
                     PVISARKFDYPAINYSESDIHAGKINFDLIGNVWFHLASMEQVIDSVHLENGYYPDSS
                     SAFYNYRETPWVDNLLWLLDSMIKEHTRAKGRYIVQKHYWPEAQQGTVCLSHSVDTLQ
                     KWSMSSLLLSVVDDLLMLFSLKWGRLFRELGGKFRYLFTNDELYWNFEEFRKLERDSK
                     CRSTWFIAAESNEYIDYTLDDPDLQEEIQHLNRENCEIGLLTTADKLNRDDFMTRKQV
                     MLHLLHKDQIGIRQLGFKTNAQLRDLHNKLSPSYSQSEAYHETPGYKNGFCIPWHPWI
                     GSAKASFPEIPLVFRDRYWVVNKYKNLQLDDAKHQLKKFFQHCMRVHGIFALDTRVAS
                     WTDLSYMDKLYPYVLALIKSTNAWITTAAEISTWWEKRSKVTIDEGEYEISVYFPDDL
                     DHFTLQIFGEAKIKEIDGVTARIEDNTIKFSAVKANSIAVVRLH"
BASE COUNT          485 a          473 c          560 g          548 t
ORIGIN      
        1 cgcgagataa atgtcttcga tgtgctgctt gaagtccaaa gccccgtcaa gcaaggaggt
       61 aaaagcctat tctgtccagg ggctttgaac tccaaggcgg ccaaggtggt attgtttttc
      121 acaagctctg aagccagtga tacaaggctc attgcagatg ctctctcatt atctcatctc
      181 tcattgttaa aaaaagccct ctgctttctc tgatttctct gaggcatgtt gataaaacac
      241 catgtgtaca actcattgtc acgcaccgac taaagtcagt gctacgaccg gctgaagcca
      301 gtgataccgt caatggagac gcaccaccgc gatggaattg gccttcacgg cgctgaactt
      361 gatggtgttg tcttcaatcc tggccgtcac gccgtcgatt tctttaatct ttgcttcccc
      421 aaagatttgg agggtaaaat ggtccaaatc atcgggaaaa tagacactga tctcatactc
      481 gccctcatcg atagtcacct tggagcgttt ttcccaccaa gtggatatct cggccgcggt
      541 ggtgatccag gcattggtgg atttaatcag tgccagcaca tagggataga gcttgtccat
      601 atagctgagg tcggtccagg aggccactct ggtgtccagt gcaaagatgc cgtggacgcg
      661 catgcagtgt tggaaaaact tcttgagctg atgtttggcg tcgtccaact ggaggttttt
      721 gtatttattt actacccaat accggtctct gaacaccagc gggatttctg gaaaactggc
      781 tttggcggat ccgatccagg gatgccaggg aatgcaaaag ccgtttttgt aaccgggagt
      841 ttcgtggtat gcttcgctct ggctgtaaga aggtgagagt ttgttgtgca ggtctctaag
      901 ctgggcgttg gtcttgaatc ccagctggcg gatgccgatc tggtctttgt gcaagaggtg
      961 cagcatcacc tgcttgcggg tcataaagtc gtcccggttg agtttatcgg cagtggtgag
     1021 cagtccgatt tcgcaattct cgcgattgag gtgttggatc tcttcctgga ggtcgggatc
     1081 atccagcgtg tagtctatat attcattgct ttcggcggcg ataaaccagg tgctgcggca
     1141 tttggaatcg cgttccagct tgcgaaactc ttcaaaattc caatagagct catcgttggt
     1201 gaagaggtag cggaatttgc cgcccagttc gcgaaagaga cggccccact tgagactgaa
     1261 cagcatcaac agatcgtcca ctacagagag cagcaaggaa gacatgctcc atttttgcag
     1321 ggtatcgaca ctatggctga ggcacacggt cccctgctga gcttcaggcc agtagtgctt
     1381 ttgcacgatg tagcgtccct tggcgcgggt gtgttctttg atcatggaat ccagcagcca
     1441 gaggaggtta tccacccagg gagtctcgcg gtagttgtaa aaagcgctgg aactgtccgg
     1501 atagtagccg ttttcgaggt ggacgctgtc gatcacctgc tccatcgagg ccagatgaaa
     1561 ccagacgttg ccaatcaggt caaaattgat cttgccggcg tggatgtcgc tctcggagta
     1621 gttgatggcg ggatagtcaa acttgcgagc cgaaattacc ggagtggggg agagcagctt
     1681 gatctcttta aggttctttc tcagcttttc gggtgtataa ccttggggat caaagagatc
     1741 aggatcgctc tgaataaaga tggtgatgta gtgtcgcgcg atgctaccca actcttccat
     1801 ggtgggatcg gtgtagccgt agatcatcag gatgtcatta tccttgagct ggcccgtatc
     1861 cgagataaag cagtggctgt agcccagagt ggagagcaca aaactgaagg cgtacttgat
     1921 ttcgcgctga taacgctgca gtttgaagtc tatatatatg gatatcatct tagctttgaa
     1981 ccaccaaatt cagtttattg ccttaaaaac ttgcttaagc ggcttgtcaa gcgaaatcgc
     2041 tgaagtttga tagaacgcag atgaca
//
LOCUS       DAOSFN010000220         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_59173_length_1943_cov_8.966631, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000220 DAOSFN010000000
VERSION     DAOSFN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59173_length_1943_cov_8.966631"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..1470
                     /locus_tag="PLO35_09665"
     CDS             340..1470
                     /locus_tag="PLO35_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH79976.1"
                     /translation="MENPFQYGKVVSDPSFTDRRQELREMLAEIQSLHSLVLYSPRRY
                     GKTSLLCKVAEQARDLGYKTIFIDFFRVNSRAAFVEIYAREILGQYRKSWRTILKKMA
                     SLASGIRPKVSLDTFGNLSFSIDIEAQGDQRLDLEAVLDLPESLDPQQKWLIIFDEFQ
                     ELAKLNGNSFEDLLRARLQHHQRSSYIFCGSQYHLLLKMFNLPSRPFYRFGKLMRLEK
                     IAPAVMADFVWERFGATNTPISREMAQHIVRLADNIPNYVQYIASEFWQLLRLSGRQP
                     SEDLLQEAMQKIIQSQKDYFLQIWDGLSPQQRKLLLALSEENSNIFSEEYHKRHRLGA
                     ISSSQTAARKLVADQLIVKEGFAYLFADPLFRQYLKTQMTIS"
     gene            1499..1858
                     /locus_tag="PLO35_09670"
     CDS             1499..1858
                     /locus_tag="PLO35_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79977.1"
                     /translation="MKDWKKALGLALVLTAISTTLSVAITLIIKAKKAHSLKFKELLR
                     ELYPQHEWEHTGLYIHLWDVVYKAWYAEEFDYREGETEAEVIDAINVRAPGLKPKVEF
                     RPIAEFEEMIKGKCCFK"
BASE COUNT          521 a          477 c          510 g          435 t
ORIGIN      
        1 tgcatgtatt tataactcat tatcatcgta ttagtctaat ctaaactgcc gtccccatgg
       61 gactcaatgc gccattttcg cacaagttct tcagccggtc gtagcactga ctttagtcgg
      121 tgcgtgataa aatactatct gctttcacat caatggtgta tcactcagag tctggatcgc
      181 ggggtaccgg ctaaagccgg cacaacgacc ggctgaagcc agtgatacca attattcaac
      241 ggtctcatcc attatgtaag attgcattat gtagaacaac ataatttggc ttgacatgat
      301 cgcgcctttc cactttatgg aaaccgggag gtgaaacaca tggaaaaccc ctttcaatat
      361 ggcaaagtgg tgagtgatcc cagcttcaca gacaggcggc aggagctgag ggagatgctc
      421 gcggagatcc agagtctgca cagtctggtg ctctattctc cccgcagata cggaaagacg
      481 tcgctgctct gcaaggtggc tgaacaggcc agagatctgg gctacaagac gatcttcata
      541 gattttttca gggtcaatag ccgggccgcg tttgtagaaa tctatgccag agagatactt
      601 ggccaatacc gcaaaagctg gcgaacaatc ttgaagaaaa tggccagcct ggcatcaggg
      661 atcaggccca aagtgagcct ggacaccttt ggcaacctca gcttctccat cgatatcgaa
      721 gcccagggag atcagcggct tgatctggaa gcggtcctgg acctcccgga atcgctggac
      781 ccccagcaga aatggctgat catcttcgat gagtttcagg agctggcaaa gctgaacggc
      841 aacagctttg aagacctgct gcgggcccgt ctgcagcacc atcaacgcag ctcatacatc
      901 ttctgcggca gccagtatca tctgctattg aagatgttca acctgcccag ccggcccttc
      961 tacagatttg gcaaacttat gcgcctggaa aagatcgctc ccgctgtgat ggcggatttt
     1021 gtctgggaac gctttggcgc taccaatacc ccaatcagcc gggaaatggc ccaacatatt
     1081 gtccggctgg cggataacat ccccaattac gtgcagtata tcgcctccga attctggcaa
     1141 ctgctcaggc tcagcggccg tcaaccctct gaggacctct tgcaggaagc catgcaaaag
     1201 atcattcaga gccagaagga ctattttttg cagatttggg atgggctgag tccccagcaa
     1261 agaaaactgc tgctggcgct gagcgaggaa aacagcaata tcttttccga ggaatatcac
     1321 aaacgccatc ggctgggggc catatcttcc tcgcagaccg cggcgcgtaa gctggtggca
     1381 gaccagttga ttgttaagga aggatttgcc tacctgtttg ccgatcccct gttcagacag
     1441 tatctgaaga cacagatgac catctcttag agctggattt tcacgcatag gagaaacaat
     1501 gaaagactgg aaaaaagccc tgggactggc gctggtcctc acggccatca gcactacgct
     1561 gagcgtggcg atcacgttga tcatcaaggc caaaaaggcg cacagcctga aattcaagga
     1621 actgctcagg gagctgtatc cgcaacatga atgggaacat acgggattgt atatccacct
     1681 ctgggacgtg gtttacaaag catggtatgc ggaggagttt gactatcgcg aaggcgaaac
     1741 ggaagccgag gtgatagacg cgatcaacgt cagagcgcca ggtttgaagc ccaaggtgga
     1801 gttcagaccg atagcggaat ttgaggagat gataaagggg aaatgctgtt ttaagtagag
     1861 cggcaaacat cccatttagc cagatcaata gaacgcggat gacacgatgt tatcaattga
     1921 gaattgagga ttgagaattg agg
//
LOCUS       DAOSFN010000221         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_60281_length_1920_cov_9.807507, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000221 DAOSFN010000000
VERSION     DAOSFN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60281_length_1920_cov_9.807507"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..650)
                     /locus_tag="PLO35_09675"
     CDS             complement(75..650)
                     /locus_tag="PLO35_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOH79978.1"
                     /translation="MAVKNIKPVPELLGMWKAVNLVSFLILLIFPLLMFVFGAPLELS
                     LGGLFLLLVLYLCLVLYIPAFFGTLEYSISQEAVLLRKGVFWKRRTTVPYAKITNIDI
                     TQGPVERYYGLSKLHIQTAGASAANNTTAELMMYGIADPEALKDEIMARVLKPASEPV
                     TPIAAIDSDPNLLSDILAELRAIRQNIESRG"
     gene            complement(909..>1920)
                     /locus_tag="PLO35_09680"
     CDS             complement(909..>1920)
                     /locus_tag="PLO35_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH79979.1"
                     /translation="CSSIWGGTFPTIPYAVNKDGKGPAWANSLFEDNAEYGFGMRLAI
                     NSHRKLLKFTMEQLMDKAIDPALKEALGYALEHWKDVDAAAKDNAAKLKALLPAAIEK
                     ACEGCAKLLRCVSELQDYLVDKSVWALGGDGWAYDIGYGGLDHVMASNQNINIFVLDT
                     EVYSNTGGQASKSTPLGSIARFAEAGKNTNKKDLGMMMMSYGYVYVAAIAMGANKVQA
                     MNAIMEAEAYPGPSIIIAYAPCINHGIDMSQSQKRQKLAVDTGYWLLYRYNPLNRLQG
                     KNPLTLDSKEPTLPVTEFLKGERRYAGLESIFPERFEQFEGCSELFFKERYQQYKKLL
                     QD"
BASE COUNT          423 a          492 c          563 g          442 t
ORIGIN      
        1 ctacgggtgc tctacgggtc ggacccgtag agcacccgta atgggatgcg gcaagcaatt
       61 atcgcgaaga accctcagcc ccgggattcg atattttgcc ggatggcacg tagttccgcg
      121 agaatgtctg agagcagatt ggggtctgaa tcaattgccg cgatgggagt tacaggttcg
      181 gacgccggct tgaggacccg ggccatgatc tcgtctttga gggcttcggg atcggcaatg
      241 ccatacatca tcagttcggc ggtggtgttg ttggcagcgc tggccccggc cgtctgaata
      301 tgcagcttgc tgaggccgta atatctctcg accggtccct gggtgatgtc gatattggtg
      361 attttggcat aggggacagt ggtgcggcgc ttccaaaaca cccctttgcg cagcaaaacc
      421 gcttcctggg agatactgta ttccagggtg ccgaaaaagg ccggtatata aagcaccagg
      481 catagataca gcaccagcag cagaaacagc cctcccaagc tcagctccag aggagcccca
      541 aacacaaaca taaggagggg aaagatcagc aagatcaaaa atgacacgag gttgactgcc
      601 ttccacatac ccagaagttc gggaacaggt ttgatattct tgacggccat aacaagctcc
      661 tgtaaaggat tattttgcat aggtatatcc ggacgaggca tgaaggatca cgcccttgag
      721 acaggataag cataatccca gcccgggcaa gtgccatcag taagccggac cagaggcaat
      781 tcgctctatc catccctggg ggctcactct gtggtcagcc aataacgctg cagttcttac
      841 gcttcttcaa aaagataccg ccccaggatt ttcagcccgg ggcggtaggt tatttgttcg
      901 agttattttt agtcttgcaa gagcttctta tactgctggt aacgctcctt gaagaagagc
      961 tcgctgcagc cttcgaactg ctcaaagcgt tccgggaaga tagattccag gccggcatag
     1021 cggcgttcgc ctttgaggaa ttcggtgacg ggcagggtgg gctctttact gtccagagtg
     1081 agcggattct tgccctggag gcggttgagc ggattgtagc gatatagcag ccagtaaccg
     1141 gtatcgacgg ccagcttctg acgtttctgg ctctgggaca tatcgatgcc gtggttgatg
     1201 cagggcgcgt aggcaatgat gatggagggg cccggatagg cttccgcttc cataatggcg
     1261 ttcatggcct gcaccttgtt ggctcccatg gcgatagcgg ctacatacac atagccatag
     1321 ctcatcatca tcatgccgag gtctttcttg ttggtgttct ttccggcttc ggcaaaacgg
     1381 gcaatggaac caaggggagt gctcttggag gcctggccac cggtgttgga atagacttcg
     1441 gtatccagga cgaagatgtt gatgttctga tttgaggcca tcacgtgatc cagaccgcca
     1501 tagccaatgt cataagccca accgtctcca cccagggccc agactgactt gtccaccaga
     1561 taatcctgca gttcgctcac gcagcgcagc agtttggcac agccttcgca ggccttttcg
     1621 atggcagcgg gaagcagcgc tttgagtttg gcggcattgt ccttggcggc ggcatccacg
     1681 tccttccagt gttccagggc atagccgagg gcttccttca gggcgggatc gatggctttg
     1741 tccatcagct gctccatagt gaatttcagc agcttgcgat gggaattgat ggccagacgc
     1801 atgccaaatc cgtattcggc gttgtcttca aagaggctgt tggcccaggc aggacccttg
     1861 ccgtctttgt tgacggcgta agggatggtg gggaaggtgc cgccccagat ggaggagcag
//
LOCUS       DAOSFN010000222         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_65280_length_1823_cov_7.535633, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000222 DAOSFN010000000
VERSION     DAOSFN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65280_length_1823_cov_7.535633"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..>1823)
                     /locus_tag="PLO35_09685"
     CDS             complement(162..>1823)
                     /locus_tag="PLO35_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOH79980.1"
                     /translation="GLSWTEIATNLPAGNGSYDWTVPDTPGGTCHLRVSSSSNPAVDD
                     WTDNPFLISRLQLLSPNGGEIWGRQTVKTISWSSEQVTTVTLEYSLDDGVTWIQIAPS
                     LAASAHSYNWTLPAVSSTHCRVRITDTEFAHVYDASDQPFNIRPWIILTAPNGGEYLT
                     VNGIYSIQWSSLENVSQVQLDYSINGGSSWLAVQSTPVAASTGSYDWIVPDNPSANCR
                     VRVSHPTYTSVYDVSDAAFTIVSSIQPPEVQFSADLISGPAPLSVQFTDQSDPGTGSI
                     INWAWDFGDGNTSTLPNPQHIYAGAGLYSVTLSVTNAAGANSSLMRGDYINVLPGHPV
                     IVTVPDGALDFGNVYIGSQSEPFPLWIKNTGTAILNVSDITFGTAGSPFWMIPVNLPV
                     NVAGGDSIAVLIVFSPQVAGPAIDQLTIYNNSNNQPLFTLELIGSGEYVPPKPPQGVA
                     ATQDGENVVISWEAVTETELGTPIVPDYYLVFYNGSPDPNGLYYFHGATPSLNYTHYL
                     VGTHAQYMFYRVLAYKHCGRSPFDLSSLQTGMPERDVLQLLDDLR"
BASE COUNT          433 a          475 c          503 g          412 t
ORIGIN      
        1 tgtttgggac accggatcta tctaaactgt cgtcccgccg ggactcgtaa ggaatgatgt
       61 tacgataatc agttttcgca ccagctcgga gtcgggaatg acagcaaggc ttttcatgta
      121 aactccaacg gggccaattc tcagatttca taccagttct attacctcag atcatccagc
      181 agttgcagca catcccgttc cggcatgccg gtttgcaggc tgctgagatc aaaggggctt
      241 cggccacaat gcttgtacgc cagcacccgg taaaacatat actgggcatg agttcccacc
      301 agatagtgag tgtagttgag gctgggagta gctccgtgga aataataaag tccgttggga
      361 tcgggggaac cgttgtaaaa taccagatag taatccggga caatgggagt acccagttcc
      421 gtctccgtta cagcctccca actgatcacc acattctccc catcctgggt tgcagccact
      481 ccctgaggtg gtttgggcgg aacgtattct ccgctaccaa tcagctccaa ggtaaataag
      541 ggctggttat tggaattatt gtaaatggta agctggtcga tcgcaggccc tgccacctga
      601 ggcgagaaca cgatcaggac agctatactg tctccgccag ccacattcac aggcaggttg
      661 accggaatca tccagaaggg agaaccggcc gtgccaaagg ttatatcaga cacgttcagg
      721 atagctgtgc cggtattctt gatccagaga gggaaaggct ctgattggga gccgatatac
      781 acattgccaa agtcaagcgc gccatccggc acagtgacaa tcacaggatg ccctggcaac
      841 acgttgatat aatcgccgcg catgaggcta ctgtttgcgc cagcggcgtt ggtgacacta
      901 agcgttacgc tgtataagcc cgcaccggca tagatatgct ggggattggg gagcgtggat
      961 gtgttgccat cgccaaaatc ccaggcccag ttgatgatgc tccctgtgcc gggatcgctt
     1021 tgatcggtga attgcacgct caatggcgct ggtccactaa taagatcagc actgaattgc
     1081 acctccgggg gctggatgga acttacaatt gtgaatgccg cgtccgatac gtcataaaca
     1141 ctggtatagg ttggatggct tacccgcacc cggcaattgg cagaaggatt atccggcact
     1201 atccagtcat aggagccggt ggatgcggcc acgggggtac tctgaacggc aagccagctc
     1261 gatccgccat ttatgctata atccagctgc acctgcgaga cattctccag gctggaccat
     1321 tgaatactgt atatgccgtt cacagtgagg tattcacccc cgttgggtgc cgtcaggatg
     1381 atccagggcc ggatattaaa gggctgatcg ctggcgtcat agacgtgagc gaattccgtg
     1441 tctgtgatcc tgaccctgca atgggtggaa ctcaccgccg gcaaagtcca gttatagcta
     1501 tgcgcggagg ctgccaggct gggcgcaatc tggatccagg taactccgtc gtctaatgaa
     1561 tactccagtg tcacagtggt cacctgttca gaagaccagg atatggtctt cacggtttgc
     1621 cttccccaaa tctcaccccc gttcgggctc agcaactgca gtctgctgat caggaaagga
     1681 ttgtcggtcc agtcatccac agcgggattg gaagaagagg acacacgcag atggcaggtt
     1741 ccgccaggtg tgtcaggcac tgtccaatca taggagccat tgccagcggg aagattggtg
     1801 gcgatttctg tccagcttag ccc
//
LOCUS       DAOSFN010000223         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_67617_length_1780_cov_10.053913, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000223 DAOSFN010000000
VERSION     DAOSFN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67617_length_1780_cov_10.053913"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /gene="hydF"
                     /locus_tag="PLO35_09690"
     CDS             complement(<1..1193)
                     /gene="hydF"
                     /locus_tag="PLO35_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOH79981.1"
                     /translation="MSVSIRGERLVITLLGNRNAGKSSLINAIVGQEIAIVSDTPGTT
                     TDPVDKRYELIPIGPVTFYDTAGLDDEGELGQKRVKATLKVLFRTDMAVLVNDGSPFN
                     PKERETIARVKEMEIPLLVVFNKGDLGVPATTNIEFCSNHSLRHITVSSSNQEHIEEL
                     KELLIELAPLHLKQEKVLVGDLIKAGDKIILVTPIDKSAPKGRMILPQVQVLRDLLDY
                     NAVVTFAKETELALALEALKEPPALVVTDSQAIELVAKIVPLDVPLTTFSILFARYKG
                     ELEILAQGVRQIDHLQPGDKVLIAEACSHHVQDDDIGKYKLPRWIEKYLGFELVFESA
                     QGHDFPDNLEDYKLVIHCGACMLNQAEMGRRIMEAHRRHVPISNYGLAITKVQGVFER
                     CVQVYG"
     gene            complement(1262..>1780)
                     /locus_tag="PLO35_09695"
     CDS             complement(1262..>1780)
                     /locus_tag="PLO35_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH79982.1"
                     /translation="ADVARQFGEISYLLPASGLTALAGYAFHDPKTADTGFLALKALL
                     IAGATTRSLQLLTQRQRPFQEQGHEFWNGRGIKWDRDSFPSGHSTVAWTVAPILAYQY
                     SHHAWVAPVVYSLATLTSLSRIYEDKHWSSDVFAGAVVGMLAARLTLISTPRLSVVPC
                     VVQPGISLIYTF"
BASE COUNT          365 a          463 c          537 g          415 t
ORIGIN      
        1 ccatatacct gcacgcagcg ttcaaacaca ccctgcactt tggtgatggc cagtccatag
       61 ttgctgatgg gcacgtggcg gcgatgagct tccatgatgc ggcgtcccat ctcagcctgg
      121 ttgagcatgc aagccccgca atggatcacc agcttgtagt cttccaggtt gtctggaaag
      181 tcatggccct gggcggattc aaagaccagt tcaaagccca ggtatttctc tatccagcgg
      241 ggtagcttgt atttgccgat atcgtcgtcc tgcacgtgat gcgaacaggc ttcggcgatc
      301 agcaccttgt cgccgggctg gagatggtcg atctggcgca cgccctgggc caggatctcc
      361 agttcgcctt tgtagcgggc aaagaggatg gaaaaagtgg tgagcggcac atccagcggc
      421 acgatcttcg ccaccagttc gatcgcctgg gaatcagtta ctactagggc cggtggctct
      481 ttcagagcct ccagggccag ggccagttcc gtctctttgg cgaaggttac cacggcattg
      541 tagtccagca ggtctctcag cacctgcacc tgaggcagga tcatacgtcc cttgggggcg
      601 gatttgtcga tcggggtcac cagaatgatc ttgtcgccag ccttgatcag atcgcccacc
      661 agcacttttt cctgtttgag atgcaggggg gccagctcga tcagcagttc cttgagttct
      721 tcgatgtgtt cctgattgct ggatgacacg gtgatatgac gcaggctgtg attgctgcag
      781 aattcgatgt tggtggtggc ggggactccc agatcgcctt tgttgaagac taccaggagg
      841 ggaatttcca tttcctttac cctggcgatg gtttcccttt ctttgggatt gaagggactg
      901 ccgtcgttga ccagaacggc catgtcggtg cgaaaaagca ccttgagggt ggccttgacg
      961 cgcttttggc ccagctcgcc ttcatcatcc aatcccgctg tgtcatagaa ggttacgggg
     1021 ccgatgggaa tgagctcata gcgtttgtcc acaggatcgg tagtggtgcc gggagtatcg
     1081 gaaacgatgg cgatctcctg accgacaatg gcatttatga ggctggactt tccggcattg
     1141 cggttgccca gcagagtgat gacgaggcgt tcgccacgga tgctgacgct catggttcct
     1201 ccatgattat gattgtgtga gccttgattc ctgagggtaa gagctttgtc aagtcttccc
     1261 ctcaaaaggt gtaaatcaat gatatcccag gctggaccac gcatggcacg acactcagtc
     1321 tgggagtgga gatcaatgtg agccgggcgg ccagcattcc tactacagca ccggcaaaga
     1381 catcgctgct ccagtgtttg tcttcataga ttctggatag cgaggtgaga gtggccaggc
     1441 tgtaaaccac tggagccacc caggcatggt gtgaatactg ataagccaga atgggagcga
     1501 cggtccaggc cacggtggag tgtccggagg gaaaggaatc gcggtcccac ttgatcccgc
     1561 ggccgttcca gaactcatgc ccctgctcct gaaagggtct ctgacgctgg gtgagcagtt
     1621 gcaggctgcg ggttgtggcc cctgcaatga ggagggcttt cagggccaga aaacctgtgt
     1681 cggcggtttt gggatcatgg aaagcatagc cagctagagc agttaaacct gaggcgggaa
     1741 gcagatagct gatctcgcca aactgcctgg ccacatccgc
//
LOCUS       DAOSFN010000224         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_67679_length_1779_cov_10.193735, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000224 DAOSFN010000000
VERSION     DAOSFN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67679_length_1779_cov_10.193735"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..814
                     /locus_tag="PLO35_09700"
     CDS             170..814
                     /locus_tag="PLO35_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOH79983.1"
                     /translation="MALLLKTTRFVESQIDTFLDVVSDSALLLQLGMEDYLHARTAQF
                     EERLGQIRENEKKADDLRVAIERYLYERTLIPENRGDVLAILENTDEVVDNIKDSLMQ
                     FSIELPAIPEELDDLWMQTTRASVAAVEQLVMAVRSFFRDIAAVNNYIHKVYFFEREA
                     DHLGEKLRRQIFSLPIELAHKSQLRYFAIHIEKISDSAQAVCDRLAIYTIKRQL"
     gene            815..>1779
                     /locus_tag="PLO35_09705"
     CDS             815..>1779
                     /locus_tag="PLO35_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOH79984.1"
                     /translation="MIFIYLISGLFLGWSLGANDTGNIFGAAVETRMLKFKKAALIAS
                     VFIFLGAVLEGSGPSGTLGRLGSVDALGGAFTVSLAAAAAIFVIVRLGIPVSTSQTIV
                     GAIIGWNFFSGRITDFRSLLTIASSWVTAFVLSAAIAALLFYLFKAWISRSSMHLLEL
                     DLFVRYSLIVVGAFGAYSLGANNIANVVGVFVPVTPFKDLNLAGLITLDGVQQLYILG
                     AISIVVGIYTYSHKVMRTVGKDLFQLSPVTALVALMAEAIVLFLFASRGLYKLLLSMG
                     LPTIPLVPVSSTQVIVGAVMGIALAKGGKNLRYNILGRISLAWVTA"
BASE COUNT          362 a          496 c          483 g          438 t
ORIGIN      
        1 gtatctcacc tacgcagtat ctcacctacc gcaggtatct cacctacgca gtatctcacc
       61 tagcgcaggt atctcaccgc gaagctccat ttcccgcttg acaaaaaccc gccctgaaaa
      121 atatcttgaa aggttatgcc ccgcaaataa aagcacaggg agatagacca tggcattgct
      181 gctcaagacc acgcgctttg tggaatcgca gatcgacacc tttttggatg tggtgagcga
      241 ttccgccctg ctcttacaac tgggtatgga agactatctg catgcccgca ccgctcagtt
      301 tgaggaacgc ctcggccaga ttcgtgaaaa cgaaaagaag gctgacgacc tcagagtggc
      361 gatcgagcgt tatctctatg agcgcactct catccctgaa aaccggggcg acgtgctggc
      421 tattctggaa aataccgacg aagtggtgga caacatcaag gacagtctga tgcagttttc
      481 cattgagctg cctgccattc cggaagagct ggacgacctg tggatgcaga ccacccgcgc
      541 ttccgtggcg gccgtggaac agctagtgat ggcggtccgc agtttcttta gagatattgc
      601 cgcggtgaac aactatatcc acaaagtcta tttctttgag cgggaagcgg accatctggg
      661 tgaaaagctg cgccgccaga tatttagcct gccgatcgaa ctggcccaca aaagccagct
      721 gcgctatttt gccatccaca tcgagaaaat ctcagattcc gctcaggccg tctgcgaccg
      781 tctggctatc tacaccatca aacgccagct ctaaatgatc tttatctatc tgatcagcgg
      841 actttttctg ggttggagcc tgggggccaa cgacaccggc aatatcttcg gggccgcggt
      901 cgaaacccgt atgctcaagt tcaaaaaagc cgccctgatc gcctcagtat ttatctttct
      961 gggagcggta ctggagggca gtggcccttc cggcactttg ggacgtctgg gcagcgtgga
     1021 tgctctggga ggagctttca ccgtctcttt ggctgccgcg gcggctatct ttgtgatcgt
     1081 gcggctggga atccccgtct cgacttcaca gaccatcgtg ggtgccatca tcggctggaa
     1141 cttcttttcc ggcaggatta cagactttcg cagcctgctc acaattgcgt cttcctgggt
     1201 gacggctttt gtgctgtcag cggctatcgc ggccctgctg ttctatctct ttaaagcctg
     1261 gatcagccgc agcagcatgc acctattgga actggaccta tttgtgcgct acagcctcat
     1321 cgtggtcggt gcttttggag cttattcgct gggggccaac aacatcgcca acgtggtggg
     1381 tgtctttgtg ccggtcacgc ctttcaaaga cctcaacctg gcgggactga tcaccctgga
     1441 cggagtgcag cagctctata tcctgggcgc aatctccatc gtggttggca tttacactta
     1501 ctcccacaag gtgatgcgca ccgtggggaa ggatctcttt cagttgtctc cggtcacggc
     1561 cctggtggct ttgatggcgg aggcgatcgt gcttttcctg tttgcctcgc ggggcttgta
     1621 taagctgctg ttgagcatgg gtttacctac cattccactg gtgccggtat cttccacgca
     1681 ggtgattgtg ggcgcggtga tgggcatcgc gcttgccaag ggtggtaaaa acttgcgcta
     1741 caacatcctg ggccgcattt ccctggcctg ggtcacggc
//
LOCUS       DAOSFN010000225         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_67684_length_1779_cov_8.026682, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000225 DAOSFN010000000
VERSION     DAOSFN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67684_length_1779_cov_8.026682"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..965)
                     /locus_tag="PLO35_09710"
     CDS             complement(54..965)
                     /locus_tag="PLO35_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH79985.1"
                     /translation="MKQLSEFRSHLYCLLTIAIWSTLEVVGKLLGTSVSPYAITAWRF
                     LIGGLVILPFALRNRGDIKLGLKGIAHLSALGILNVCLAMLILQLSIHYGKASVSAVI
                     ISVNPLFVAMFAMLLLKEKMGIAQILGLILGVTGIVILVLGEVEMRNVRYLNLPLGIG
                     LAIVAALAFGTYTVLTRAAVARYGNLLSNSTSFLAGSISLFAYNIIAGEQFTIQPTLP
                     NLLLAGYLGLILTGLAYLLYFEGMKRIGAGKASMYFFTKPLISTFLAWMLLGETLRAS
                     QLAAVVLIVSAMNLPRLQNLFSKGTSH"
     gene            complement(1033..>1779)
                     /locus_tag="PLO35_09715"
     CDS             complement(1033..>1779)
                     /locus_tag="PLO35_09715"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOH79986.1"
                     /translation="DDTAGVITTNYNVGDAIVGLTGTIARYNNMLQLTPVANPGPPAS
                     TGNGVFVPVVTIQQVNGNIEMYQSRLVRINNAHFVGASGAFANGTNYNLEDASGTIVF
                     RSTFYDVDYIGTDIPTGNFSLVSIVNQFNQTPQLTARNLADWGAVDNDDYVANPNQTE
                     LLGNYPNPFNPKTTIEFNAAKSEPAQITIYNLKGQAVKHFTIDSPAKGINKVTWDGKN
                     DNGIDVASGTYLFRLRSGSYSSTKKMIMMK"
BASE COUNT          435 a          439 c          494 g          411 t
ORIGIN      
        1 cggctggtta gagcctttgg ctggttagag cactgcgtgc tggttagagg cagttagtga
       61 ctggtgccct tgctgaataa gttctgcagc cggggaagat tcatggccga gacgatcagg
      121 accaccgcgg caagctgtga agcccgcaat gtctcaccca gcagcatcca ggccagaaat
      181 gtggagatta aaggtttggt aaagaaatac atagacgctt tgcctgctcc gatccgcttc
      241 atcccttcaa aataaaggag ataggccaag ccggtgagga tcaatcccaa atatcctgcc
      301 agcaagagat tgggtagcgt gggctgaatc gtgaattgct ctcccgctat tatgttatac
      361 gcaaacagac tgatagaacc agccagaaag gaagtggagt tgctcagcaa gttgccataa
      421 cgtgccaccg cggcacgagt caaaactgta taagttccaa aagccagagc cgccacgatt
      481 gccagtccga tccccagagg caggttcaga tagcgcacat tgcgcatttc cacctctccc
      541 agcaccagaa tcacgatgcc cgtcacgccc aggatcaatc ccaatatctg agcgatgccc
      601 atcttttctt tcagcaacag catggcaaac atagccacga acagaggatt gacgctgatg
      661 atcaccgcgc tgacggatgc tttgccatag tggatggaga gttgcaggat cagcatagcc
      721 aggcagacgt tgaggattcc cagagcacta agatgagcta tccctttgag gcccagcttg
      781 atatctcctc tgttgcggag cgcgaagggc aggatcacca gcccaccgat caaaaaacgc
      841 caggccgtga tcgcgtaggg cgatacagag gtccccaaca gcttgccgac cacctcaaga
      901 gtggaccaga tcgcgatcgt caggagacag tagaggtggc tgcgaaattc cgataactgc
      961 ttcatactta tgcaaaaacg ggagaggcca tggctaggtc tcccccgtta attatcatta
     1021 gttcccgacg cgttacttca tcatgatcat ttttttggtg ctggaatagg aaccggagcg
     1081 cagacggaac agataggtcc cgctggcaac gtcgataccg ttatcgttct tgccgtccca
     1141 ggtaacttta ttgattccct tggcaggact gtcgatcgtg aagtgtttga cagcctgacc
     1201 cttaaggttg tagatagtga tctgggcggg ttcggatttt gcggcgttga actcaatggt
     1261 ggtcttgggg ttgaaggggt tgggataatt gcccaggagc tcggtctgat tgggattggc
     1321 cacataatcg tcattgtcaa cggctcccca gtctgccaga ttgcgggctg tgagctgtgg
     1381 agtctggttg aactggttga caatagaaac caggctgaag ttgccggtgg ggatgtcggt
     1441 gccgatgtaa tccacatcat aaaaggtgct gcggaatacg attgtaccgc tggcatcttc
     1501 cagattgtag ttggtaccat tggcaaaggc tccacttgca cccacgaagt gagcgttgtt
     1561 gattctcacc agacggctct gatacatttc gatgtttcca ttcacctgct ggatggttac
     1621 cacgggcaca aacacgccgt ttccggtgga agcaggagga cctggattgg caacgggagt
     1681 gagctgtaac atgttgttat aacgggcaat agtgcctgtg aggccgacaa tcgcgtcacc
     1741 tacgttgtaa ttggtggtga tcacaccggc tgtatcgtc
//
LOCUS       DAOSFN010000226         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonadota bacterium isolate
            AMR_MDS_1778 NODE_82634_length_1561_cov_9.766268, whole genome
            shotgun sequence.
ACCESSION   DAOSFN010000226 DAOSFN010000000
VERSION     DAOSFN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863307
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonadota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonadota bacterium
            Bacteria; Candidatus Cloacimonadota.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 00:39:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,945
            CDSs (total)                      :: 1,920
            Genes (coding)                    :: 1,917
            CDSs (with protein)               :: 1,917
            Genes (RNA)                       :: 25
            tRNAs                             :: 22
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Candidatus Cloacimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82634_length_1561_cov_9.766268"
                     /isolate="AMR_MDS_1778"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030808"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLO35_09720"
     CDS             complement(<1..1241)
                     /locus_tag="PLO35_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH79987.1"
                     /translation="MRKTLLLFLILLPLWLAAQDMPFSVKAKTRFASSGMVGYETIDS
                     LTYYRARLIQEFKIWKIGIGLDLDFLFDQDYRLRVEDWDHWENVLDKFYYVKYANRRD
                     PVYVHVGGFPDLTMGNGLIMRRYSNMALYPQLRNLGFMIGANPKIPTSPSFELFSSNL
                     KKNEILSFSTRFRPIPDSTLAVIDELILGFSIVTDFNQYGNLRYVTPDSLMQFVPGLK
                     RKAATVMGAAVTLPVYRTKDYTLGVYSEYAYIMDMGSGAILPGIYADFKFAKVNLEYR
                     TYGRDFVPGFFDEDYEEERGWVDSLGVFRTKEDYVETLKPANGINGIVEGNYKDRIKA
                     SVSWQNIIGHDYKNGKSLWLKLWVDTQWKRLENFSLSYSRTNQQRLSIARINEPGTKM
                     NLSMTFRINKRWYVIGKYAET"
     gene            complement(1391..1537)
                     /locus_tag="PLO35_09725"
     CDS             complement(1391..1537)
                     /locus_tag="PLO35_09725"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH79988.1"
                     /translation="MRELSSSGSGSIRWDGKDTSGREVPAGVYLLKAVQDDRSGVTKV
                     LRLK"
BASE COUNT          362 a          397 c          432 g          370 t
ORIGIN      
        1 aaggtttcgg cgtatttgcc gatcacatac cagcgtttgt tgatcctgaa ggtcatggag
       61 aggttcatct tggtgccggg ttcattgatc ctggcgatgg aaagacgctg ctgattggtg
      121 cggctgtagc tgagtgagaa gttttccaga cgtttccact gggtgtccac ccagagtttc
      181 aaccagaggg atttgccgtt tttgtaatcg tgcccgatga tgttttgcca gctgacggaa
      241 gccttgatcc tgtccttgta gttgccctcc acgatgccgt tgatgccgtt ggcgggtttg
      301 agggtttcca catagtcttc cttggtgcgg aacactccca ggctatccac ccagccgcgt
      361 tcttcttcat agtcctcgtc aaagaagccg ggcacgaaat cgcggccgta ggtgcggtat
      421 tccaggttga ctttggcaaa cttgaaatca gcgtaaatcc caggcagaat ggccccggaa
      481 cccatgtcca tgatgtaagc gtattcggaa tacactccca gggtgtaatc tttggtacga
      541 tacaccggca gggtgacggc cgctcccatc acggtggcag ccttgcgttt caagccaggc
      601 acaaactgca tcagggaatc gggagtcaca tatctgaggt tgccatactg gttgaaatcg
      661 gtcacgatag aaaagcccag gatcagctca tcgatcacgg cgagggtgga atccgggatg
      721 gggcgaaagc gggtggagaa agacaggatc tcgtttttct tgaggttgga agagaagagc
      781 tcaaagctgg gagaggtggg tattttggga ttagcgccga tcatgaatcc caggttgcgc
      841 agctggggat agagcgccat gttagagtat ctgcgcatga tcaacccgtt gcccatggtg
      901 aggtctggaa agccgcccac atgcacatag acgggatctc tgcgattggc gtatttgaca
      961 tagtaaaact tatccaggac gttttcccag tggtcccagt cctccactct caagcggtag
     1021 tcttgatcga agagaaagtc caggtccagc ccgatgccga tcttccagat cttgaattcc
     1081 tgaatgaggc gggcccggta ataggtgagg gaatcgatgg tctcatagcc caccatcccg
     1141 gatgaggcaa agcgggtctt ggccttgacg ctgaagggca tgtcctgggc tgccagccac
     1201 aagggcaaca gaataaggaa taaaagtaaa gtctttctca tatagtctcc caagcatctg
     1261 gaaaagagca tgttatctgc aaatcacaag ctggagcgat acagccatcc tgtcaataga
     1321 ttattggcga aagcacccca aaaaagatgg agcgcctctc ccgacgctcc atccattgat
     1381 caaacaagtt ctatttcagc ctcagcacct tggtcacacc actgcggtca tcctgcactg
     1441 ccttcagcag atagactccg gccgggactt ctctgccgct ggtgtccttg ccgtcccaac
     1501 ggatgctgcc agagccgctg gaacttaact ccctgatctt ctggccacgc aggttgtgga
     1561 t
//
